<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="https://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Y6</accession>
    <name>T1G9Y6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bee-milk protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major royal jelly protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Y6"/>
    <dbReference type="STRING" id="36166.T1G9Y6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000028-RA">
        <property type="protein sequence ID" value="MESCA000028-PA"/>
        <property type="gene ID" value="MESCA000028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062791_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="MRJP/yellow-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009:SF7">
        <property type="entry name" value="GH10609P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PROTEIN YELLOW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29705" checksum="A8AB4B694C83AD71" modified="2013-10-16" version="1">MEFQFPSVRDEREAAAKGIFSIYGPRNQPPYDIDIQYFKNDKLIRRYEFPEEQISSVSGKTALGRVEVATKNGVCNETMAYVSDFFQPALLVYSFSDDILGEALSAENEYIVSTDILNNKNNWNDQNESRKMALDGYFQSLGSRETRCSTGVMDSSFNYYCIAEKYSAISYWNLKHPETGTNLLLTDKTYLSDPGCLKIIVAPDGNEELFIVKSSIIKVLTGNYNSQKFNFFLSSCRITDLLAKRNCTTFLCPHGKCENQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAQ8</accession>
    <name>T1GAQ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000318-RA">
        <property type="protein sequence ID" value="MESCA000318-PA"/>
        <property type="gene ID" value="MESCA000318"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778757_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000318-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7874" checksum="220B37FE66BD6A90" modified="2013-10-16" version="1">MDQDILGTCEKITSIINPTKTTIDLKEPLCEELNRFLSNFPRILLLTSFPDLFSILAWNLDSEGEQMTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB60</accession>
    <name>T1GB60_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024375">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB60"/>
    <dbReference type="STRING" id="36166.T1GB60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000481-RA">
        <property type="protein sequence ID" value="MESCA000481-PA"/>
        <property type="gene ID" value="MESCA000481"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_558506_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051968">
        <property type="entry name" value="ZnFinger_Homeobox_TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="272"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="274"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="186"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000481-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="489" mass="55473" checksum="68AEAD830482236E" modified="2013-10-16" version="1">VVKHWFRNTLFKERQRNKDSPYNFANPPSTTLNLEEYERTGQTSLQNAEKSSSTLDDQRGSSNSLIQPEYSISTSAEQEPENSSSSLDNMQIKVESIENELPANSSETENRSESESSERDVISRPQTPGLVELIGQDNLHQMGPPKKFQGSNSSLNSPAGGKRANRTRFTDYQIKVLQEFFENNSYPKDSDLEYLNASPRERTPSPQPQQNVIPSQNLIQQSNTPNSSISSSQDFYESPLSSPTTIETTISVVESGQGHQNQQQPLQPQLHNNHKRLRTTILPDQLNLLYECYQTESNPSRKMLEEISKKVNLKKRVVQVWFQNARAKDKKSRNNRLIDECNVNQNSNLTSSTPPTNMALVRELMTKDKCMVNGVFNHFFLNVPLTSTANSSEEDLEEENVKIEDTSKATNSSSLYPYYNNYGLNPDTLEPPFTLDMNPGNIFTCLICENFHTNSLDDLNNHILSDRSRPQTPTSDIMLVVNNNYICRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBD8</accession>
    <name>T1GBD8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SprT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBD8"/>
    <dbReference type="STRING" id="36166.T1GBD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000570-RA">
        <property type="protein sequence ID" value="MESCA000570-PA"/>
        <property type="gene ID" value="MESCA000570"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156104_0_0_1"/>
    <dbReference type="OMA" id="HCVELEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044245">
        <property type="entry name" value="Spartan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220:SF0">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SprT-like" evidence="1">
        <location>
            <begin position="68"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000570-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15751" checksum="767D6BB23F69C194" modified="2013-10-16" version="1">MIKTRGESADFKSNNYEAPLDKLVKSYQAPRSKAKRTITDVENEEDLNATKFLVHPMWESLDPTPDVHSLFGAFNSKFFQSRLHCVELEWSKRMYSCAGICYSRRNRMGHQVTIRLSEPLLKLRSRKDLVETLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBJ3</accession>
    <name>T1GBJ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000627-RA">
        <property type="protein sequence ID" value="MESCA000627-PA"/>
        <property type="gene ID" value="MESCA000627"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796899_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000627-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7518" checksum="CE24444CB4BAF902" modified="2013-10-16" version="1">MTGCRGLSKMSINILKIGREAMSKVIHLLTISSSILESGKEAMSISNEMMVTFEIMCPEIQPATTDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBM7</accession>
    <name>T1GBM7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="3">2.3.2.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02030017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBM7"/>
    <dbReference type="STRING" id="36166.T1GBM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000663-RA">
        <property type="protein sequence ID" value="MESCA000663-PA"/>
        <property type="gene ID" value="MESCA000663"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280607_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF6">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000663-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="12294" checksum="5A3EB3A9307B0FF9" modified="2013-10-16" version="1">MILLDLIGAANTKFHSFYPNTHRLHESLYKIERFLNQNHLLEGNNSFQFLKSYANGIVDDDHRPFLEKSMYKFYIFLINILKIYIKLLEFFIVINLEKDFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBX7</accession>
    <name>T1GBX7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBX7"/>
    <dbReference type="STRING" id="36166.T1GBX7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000768-RA">
        <property type="protein sequence ID" value="MESCA000768-PA"/>
        <property type="gene ID" value="MESCA000768"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023062_1_4_1"/>
    <dbReference type="OMA" id="PKPNWAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF399">
        <property type="entry name" value="BLOWN FUSE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="6">
        <location>
            <begin position="7"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000768-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11416" checksum="946104C9E09895AF" modified="2013-10-16" version="1">MNIQFIFTKLEHFLKIPGNSKCCDCRGSDPRWASINLGITLCIECSGVHRSLGVHYSKVRSLTLDAWESENIKVMMELGNEVVNKIYEANVPENCSMRPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC01</accession>
    <name>T1GC01_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear receptor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="Znf_NHR/GATA"/>
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        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZnF_C4"/>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="74"/>
            <end position="127"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000794-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20960" checksum="01B3158BE938A68F" modified="2013-10-16" version="1">MKLTCINSVNRPGAYPEQFQLKWFPLSPHLKQAPSIPHCSWDHKKGMKSTTYSSGMQKSASSSLRCPGQLDKGGCKSFFKRSVRRNLTYSCRGSRNCPIDQHHRNQCQYCRLRKCLKMGMRREEEYIFPNKTSQSNKKIEFRKLTFTSKLSIMLSGTLVTTHNTLDILIFYVPGTSTPIATTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCX0</accession>
    <name>T1GCX0_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="MESCA001143-PA"/>
        <property type="gene ID" value="MESCA001143"/>
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        <property type="entry name" value="PROTEIN CBG08539"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="308" mass="35208" checksum="45A0C05DE2AF9B91" modified="2013-10-16" version="1">MFLCPPSHGHGSRGWSCGIRTATTNPPIAVTPIQQFPDAPQENIHASIPQQTTEKSSTKQQQTIDLLNAVKAQLTAFSIIDRKVRRIVSNFDNINGPAIELKIEDLHFQWNRIAENHEKILTMMSGSEINVNYCIDTFCQIEDSVEDCIGNLMQKKRFQERNHLDPFTSSDRENAIGMVHLPKVNLPKFDGNHMKWHSFKTLFTDMIDAAKISDSQKLFYLKSSLIGSAAPMAEVMDDNMDNYKNAFKRLKDKYDNDRILTTVLAEKFFNQPNVKINCSQSLTEFVNDRKNVFAVLRRLKLMFLVVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD02</accession>
    <name>T1GD02_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001178-RA">
        <property type="protein sequence ID" value="MESCA001178-PA"/>
        <property type="gene ID" value="MESCA001178"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925260_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7104" checksum="FA877469776E1E9B" modified="2013-10-16" version="1">MELNRIRDNNQIRLCQAHNHECIYGNETEEILSRKCSSVVLILRICQSLNVCANPEERMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD36</accession>
    <name>T1GD36_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD36"/>
    <dbReference type="STRING" id="36166.T1GD36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001215-RA">
        <property type="protein sequence ID" value="MESCA001215-PA"/>
        <property type="gene ID" value="MESCA001215"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003791_0_0_1"/>
    <dbReference type="OMA" id="IYAQPVT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000038">
        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF142">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576829" description="Phenoloxidase-activating factor 2" evidence="7">
        <location>
            <begin position="20"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="922"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="504"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="847"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001215-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1177" mass="128749" checksum="B8289180274DA4D4" modified="2013-10-16" version="1">MKFKFVAILIAALVGTTLAGDSWAWGNKDAVDDAKPVVKESKLERRSADVEANKDEEVQGRNFVEEIIDQILRGRREGRTLGEYDQVYADPNIQNALNNGDDSQARNLIKDKLCNLGLMSCDYGEEKRPFYSQIYAQGPPPPNRMHGPPYGPPRPMPMPITSNFGGPPPPPNRYGAQPPRKVGYGFDGPPKQGPVYSGSPSVQGLGPIFSSPPASANGADIIYTKPPPGAIIYGSDKPGYEHEGGDAPYNFEHIVKDKIQVSENQQNSFYAPHPDISKTGSVLTDATLQQHVHHHYHHVDGAGAAPGVPVGASNSITADFASLAQSTGGFAPSVGALSSNSVNNFNSFESNKKETLYQSGFNSASGSLTNNFYAKPNSGLNTGIVGSQKPSGYGSFGSQSQGGFGSSGFGSSGAGSSFHSSNPDYFKKELNYNNGNNGNQFNSFGSQNKYESQFQSGYNKYDTSRNQHVDCTCVPIQQCPASDRIGRKDDLLLPIDPRNLAGKDIEALPEIDGNSTLTNTTTESPKEESKKTKRDVSAADKQSDDDEQLGDVEGRQLDYGIPAITAIGLSKRKVLPTFGISFGLPYPSGAYPHNAFGQVPAQNQHFGAISPNGLDLGLINVNPKTEFGEKVVKPLVNLHVTPNENIIHKVGSLFHKKPLVHEHYHHHDHYHPEIHYAPDFHDYHEHDHEHDHIAPVQVPPSGFGNPFPPLHGIDGGFDNHYNNFQQPESYIPQFTSPGNFHQPEPYTPQLTSPGGFGGSGYGGYGPTGGNALFSPSGSGSDFGYGYERSANVSSNVKFEDDGRDTPTAYAENYRNYRRTGKQLNDYHSIPTQAPGSWEGSSFVKFPNSRRRRSVEETESSDESVMAREKRQYYGRPQPQPQQCRVNEVCCRRPFRPPQQQQFGKCGTRNAHGITGRIKNPVYVDGDAEFGEYPWHAGILKKDPKESVYVCGGTLIDAQHIITAAHCIKSYQGFDLRVRLGEWDVNHDVEFFPYIERDVISVHVHPEYYAGTLDNDLAILKIDQPVDFQKNPHISAACLPDKFTDFTGARCWTTGWGKDAFGEYGKYQNILKEVDVPIIGHGQCQQQLRQTRLGYSYNLNPGFLCAGGEEGKDACKGDGGGPLVCERNGAWNVVGVVSWGIGCGQVNVPGVYVRVAHYLTGSTRSLTAIKDENSRNHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD80</accession>
    <name>T1GD80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD80"/>
    <dbReference type="STRING" id="36166.T1GD80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001261-RA">
        <property type="protein sequence ID" value="MESCA001261-PA"/>
        <property type="gene ID" value="MESCA001261"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117953_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40240">
        <property type="entry name" value="PLEXUS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40240:SF1">
        <property type="entry name" value="PLEXUS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="23477" checksum="C870D999B74D161B" modified="2013-10-16" version="1">MFGKCRKISHLEKKLYRQSVYHPKCKKSTTKLGGGALVCTFCYHSLLNQWRKYESQPNPPSPSERTYNWHDYICHVCSVQTYRKRVRALLVNEYPNLKNHKRCEDALLLENAEYATVCLDCYESLRMQTSQYDRLGIPLARRQFNWIQQKPPPEDSPDVSVARMPCGERSDKVQISSAMRQLPNKKNNSSPKQFSNEKAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDQ3</accession>
    <name>T1GDQ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02022057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDQ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001445-RA">
        <property type="protein sequence ID" value="MESCA001445-PA"/>
        <property type="gene ID" value="MESCA001445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801302_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16761" checksum="E65C6DD10C1094EA" modified="2013-10-16" version="1">FYNSHGIDIETEKQLKRLENNTNIHNNEKSQENVFPENYIKSRNTPFKKYIRIKINKLTQNQIDKLNAELKNNKSISTTENSKDCEIYEKLPEISNFTNGTDMGNMKNCDRSEQLQEIPTFSNEDISKEVLAKNKKVKGKTTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEX7</accession>
    <name>T1GEX7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEX7"/>
    <dbReference type="STRING" id="36166.T1GEX7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001900-RA">
        <property type="protein sequence ID" value="MESCA001900-PA"/>
        <property type="gene ID" value="MESCA001900"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1039331_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001900-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="31551" checksum="4033C288208CC33B" modified="2013-10-16" version="1">MYNNHGIEILHPFKESFSLPRITKEPRRMVRRKVTRLFRDSPDQYYLPNQCSSTNHQVNNDISKGNEENLKRLRGLFVDFQNKRAQDGANVNLVHDEFKSFKCEDCGETFHNDMAMKTHRIIKHIVQKVYECDICYKQFAFKESLNVHKEKKNCVRDLPILVPESYPKPKEIKVNFVLDFSNIKVRSPPEINSNSPQRSPSSPQHARIVVDLLPRKRCLSGIRDMSANDSRNWIVSRIYLSFKIVIYLYFFYTDKKKEKSDDDSDSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFL9</accession>
    <name>T1GFL9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002163-RA">
        <property type="protein sequence ID" value="MESCA002163-PA"/>
        <property type="gene ID" value="MESCA002163"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796898_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7568" checksum="79D8C077E1889817" modified="2013-10-16" version="1">MTKESTTYSTSQRQMQAFYYALQHPGTPSSGPATTSTKLVTSQLSDSQYRSLLETVYGSEKNNSHRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG57</accession>
    <name>T1GG57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal eL28/Mak16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02100989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG57"/>
    <dbReference type="STRING" id="36166.T1GG57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002366-RA">
        <property type="protein sequence ID" value="MESCA002366-PA"/>
        <property type="gene ID" value="MESCA002366"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050888_2_1_1"/>
    <dbReference type="OMA" id="WHCIRHG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="5">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002366-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="24193" checksum="53E179B511EB9175" modified="2013-10-16" version="1">TDTRTFCRHEYNLTGLCTRRTCPLANSQYATIREEKGIIYLYMKTAERAHFPRNLWEKVKLSRNFEKAITQINENLVFWPKYMIAKCKQRFVKITQYLIRMRKLKLRRQKLIVPLSTKIERRERRREDKALVAAKIDNHIEKELLERLKKGTYQDIYNFPQTAFNKALEAEEVEEDEEEVEEEMEVEKEMDEDVRRELLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG71</accession>
    <name>T1GG71_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Laminin IV type A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG71"/>
    <dbReference type="STRING" id="36166.T1GG71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002381-RA">
        <property type="protein sequence ID" value="MESCA002381-PA"/>
        <property type="gene ID" value="MESCA002381"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_463320_0_0_1"/>
    <dbReference type="OMA" id="CRSSTWY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000062">
        <property type="entry name" value="Terribly reduced optic lobes, isoform AI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF444">
        <property type="entry name" value="BASEMENT MEMBRANE-SPECIFIC HEPARAN SULFATE PROTEOGLYCAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00052">
        <property type="entry name" value="Laminin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00281">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51115">
        <property type="entry name" value="LAMININ_IVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin IV type A" evidence="7">
        <location>
            <begin position="399"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002381-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="630" mass="69621" checksum="B1715D21460FA46E" modified="2013-10-16" version="1">GASDVNGRLEIPNIRIEDSGDYVCQAIGFSNHIPGQSVHVSLIVEQHKFIPETHVFCNAYNESTCMDGITCIDRNFICDGRPQCPDSSDEFSCSINKKCQPNQFMCRNGKCVAKFWRCDGENDCGDNSDEESCGQTPVSGPCKNSADVILVGKRYSLFYYNNELPSSISVLLYPGKLLKANNMPATTEEVIYTLSELTNILVKISYSRQTNVILSEMSINSADTNVFKELCSCPPGYSGSSCEKCEPGYSKNRNGKCIAVQECPAGTYGDLTRDIPCTECACPGNGRNYADGCRLDDYGQVVCNCRRGYTGRRCEICDSGYIGNPLNGIECEIPRSQCNMDGTLRINPDEGAKCDRCKPSSFFLNSNSESGCINCFCSGVSSECRSSSLYRDTIYSRGSNLNDIKLSTFLADGITTHYNSVQSVLRGAELTHSRDTNDLLYWKLPDLFLNNKVKSYGGFLNYTLRYAPSQGGAMSKNAAADVIIKSANEIEIQHFRRTPLSAGQSSSYSIPLFEQLWQRKDRVVINREYFLMALSNIEAIYIKATYTTTTSEVVLNEVSMDIAIENFTGREQAYEVEECVCPEEYVGLSCEYCAPGYTRNYESGLYLGSCEPCSCNGNSNECDSETGVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGN5</accession>
    <name>T1GGN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02124450">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGN5"/>
    <dbReference type="STRING" id="36166.T1GGN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002558-RA">
        <property type="protein sequence ID" value="MESCA002558-PA"/>
        <property type="gene ID" value="MESCA002558"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_708424_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF395">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="WW" evidence="8">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="9">
        <location>
            <begin position="315"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="49"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="103"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002558-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="390" mass="42804" checksum="E9C914BF707A1683" modified="2013-10-16" version="1">MNKLENSRRNGAHKIRIVIQRLELGQLNPSDDEVIRGQIIISLVSKDGPNSGNPVRIVDPSGDFRAPATPEESSEESLPEGWEERRTPEGRLYYVNHTTKTTQWNRPTANQNGNVDENSRINGPSRSNTCTNLLNDSQSNNVDTLQLSPPQSARRHSAEILTDIDLDCPTVIANGQSPQPQSDVNVVGGESSKKTTNQQQSTSINENIENQINNLNKTPRSHSANVPQVPKTSLSPTLDNLILESPQRPTTSQSVLNGNSPATPSNENGNAINTPTTTQSSNSNHRENSSSGRIRRSSRNLDESSSNSRRRSSRGTARVPLQGFRALQGSTGAVGPRLFTIHLTSDVPTQNLPKAHTCFNRIDLPPYDNYQLMYDKLTQAVEETCGFAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGN9</accession>
    <name>T1GGN9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002562-RA">
        <property type="protein sequence ID" value="MESCA002562-PA"/>
        <property type="gene ID" value="MESCA002562"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500479_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002562-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9766" checksum="C712F5742F47D77D" modified="2013-10-16" version="1">MDGQTRFFKSHAVVIGAIGGVYVCQIVPKKYFYLPTCARPAFYTCLYVKLVVHNLTFVFVEVLGNRLKDNVLVELRSLFPYSDVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGP0</accession>
    <name>T1GGP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGP0"/>
    <dbReference type="STRING" id="36166.T1GGP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002563-RA">
        <property type="protein sequence ID" value="MESCA002563-PA"/>
        <property type="gene ID" value="MESCA002563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1596386_0_0_1"/>
    <dbReference type="OMA" id="CHRSHEV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="19145" checksum="BBFE3EBD26602432" modified="2013-10-16" version="1">MVLVEVLRKYKEENEDANIMIFTNTKKYCQLLSMTITSIGFENVCLHGFMRQKERVAALGRFKSKHTKILIATDVAARGLDIPNVQLVINHRLPHSPKEYIHRVGRTARAGRKGLAISILRFPRDLDFLYKIEEEIGTKLTEHQVDHYGSYAEPNPLTKNQLQPNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH38</accession>
    <name>T1GH38_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH38"/>
    <dbReference type="STRING" id="36166.T1GH38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002722-RA">
        <property type="protein sequence ID" value="MESCA002722-PA"/>
        <property type="gene ID" value="MESCA002722"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392225_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002722-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10681" checksum="D0CA34279C1A6436" modified="2013-10-16" version="1">MTVPPPKDDFIHPDKTIVSYLKHHPKYKKVYVLAGRVFKNALNESGVIVLDEPIFQHELTLESFSESIVPLEPIDAVIIDVSLDLTYVQYCKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH72</accession>
    <name>T1GH72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BBSome complex member BBS5 PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BBS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH72"/>
    <dbReference type="STRING" id="36166.T1GH72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002761-RA">
        <property type="protein sequence ID" value="MESCA002761-PA"/>
        <property type="gene ID" value="MESCA002761"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112613_0_0_1"/>
    <dbReference type="OMA" id="DISHSNM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006606">
        <property type="entry name" value="BBL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014003">
        <property type="entry name" value="BBS5_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351">
        <property type="entry name" value="BARDET-BIEDL SYNDROME PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07289">
        <property type="entry name" value="BBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00683">
        <property type="entry name" value="DM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BBSome complex member BBS5 PH" evidence="8">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002761-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="24041" checksum="7BBC68D6A7E88C2B" modified="2013-10-16" version="1">MKLRGAILQNSQLIILPDEQVYTQMHGVWNLSSDQGNLGSFIITNVRLVWFADLNETFNISLPFIQIGSIKIRDSKYGAALVIQTTETAGGYVLGFRIDPGEKLHEIYKELLSLHAVFCENPNFGVKYNVSEREAEEEMQSNQFSVGDYEEIDDQQDSELNSKLHSYIVGGAFGTGSSNQQREPVYCKELGFSIEKIRDGYTLQDLWNVTGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHR9</accession>
    <name>T1GHR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone acetyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="8"/>
    <dbReference type="EMBL" id="CAQQ02070034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHR9"/>
    <dbReference type="STRING" id="36166.T1GHR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002977-RA">
        <property type="protein sequence ID" value="MESCA002977-PA"/>
        <property type="gene ID" value="MESCA002977"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1604591_0_0_1"/>
    <dbReference type="OMA" id="HASKCRW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036409">
        <property type="term" value="C:histone H3-K14 acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000003">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="9">
        <location>
            <begin position="29"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002977-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="166" mass="19459" checksum="B5951562E00A7D91" modified="2013-10-16" version="1">MVRVVAAIAHVTSACASVLILTVSFPSNLLQRCDQYVIMGKYQMKVWYQSPYPDDTTRIPKMYICEFCLRYQKSEIGIKRHASKCRWRHPPGDEIYRKGKLQVWQVDGKKHKQYCQHLCLLAKFFLDHKTLYYDVEPFLFYIMTLADTDGCHTVGYFSKNFVGLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHT7</accession>
    <name>T1GHT7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHT7"/>
    <dbReference type="STRING" id="36166.T1GHT7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002998-RA">
        <property type="protein sequence ID" value="MESCA002998-PA"/>
        <property type="gene ID" value="MESCA002998"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801305_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002998-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="15600" checksum="878AC6288C4D78EE" modified="2013-10-16" version="1">MGGGRNVAECSGNKSASSNAVAKPSPGKKSKIPVPKGRKSSLPVGGAGESVEQSATSPTHSGNVLKNENEIEKENNNKEKDNALVKEQQKQEEQQQPQNPEKEEKEVDGKKETPLPKLKSCRTIIADSYYEILMTNPDILESLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJC2</accession>
    <name>T1GJC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJC2"/>
    <dbReference type="STRING" id="36166.T1GJC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003567-RA">
        <property type="protein sequence ID" value="MESCA003567-PA"/>
        <property type="gene ID" value="MESCA003567"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_607338_0_0_1"/>
    <dbReference type="OMA" id="NQEPMST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06768">
        <property type="entry name" value="PDZ_NHERF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000007">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000035">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000009">
        <property type="entry name" value="Adenosylhomocysteinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="323"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003567-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="451" mass="50296" checksum="0681B5419A44CFD9" modified="2013-10-16" version="1">MLKKYPAIFKQIKGIVEESVTGVHRLYQLSKSGKLTVPAMNVNDSVTKTKFDNLYSCKESIIDSLKRSTDIMFGGKQVVICGYGEVGKGCAQALKGLGCIVYVTEIDPICALQAGMDGYRVVKLGEIIRSIDIVVTATGNKNVVTRDLMDKMKNGCIVCNMGHSNTEIDVNSLRTPDLTWEKVRSQVDHIVWPDNKRIILLAEGRLVNLSCSSIPSFVVSVTSATQALALIELFNAPIGRYKSDVYLLPKKMDEYVASLHLPTFDAHLTELTDEQAKYMGLNKAGPFKPNYYSSLSFFDQIGRFISVAEKLKKYDLMSTSERLCHLVKRSDFDGFGFNLFSEKGKAGQFIGKIDENSPAEFAGLITGDRIIEVNGENISNESHKQVVERIKAISDEVRLLVARNQEPMSTKIQFNMTAAELRAQLQNKKKYDAKSDSAIDLRKKFEIIQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJK8</accession>
    <name>T1GJK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ephrin RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02162315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJK8"/>
    <dbReference type="STRING" id="36166.T1GJK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003657-RA">
        <property type="protein sequence ID" value="MESCA003657-PA"/>
        <property type="gene ID" value="MESCA003657"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1596387_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF29">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ephrin RBD" evidence="8">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003657-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="19043" checksum="BB0E11F0EFF6FB8F" modified="2013-10-16" version="1">MFFTITFRPFTPQPGGLEFLPGNDYYFISTSSKDDLYRRIGGRCSTNNMKVVFKVCCAQEESNHLNYTTTITSPTKLEHDESLNKANRIETDKHQHSSQSINTNISNVLRSTVSWSTPVISSTIPVRPNHYHPPFLSSPNPSYEQKEKTSVYPNKPTKKISEKNQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP1</accession>
    <name>T1GJP1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003691-RA">
        <property type="protein sequence ID" value="MESCA003691-PA"/>
        <property type="gene ID" value="MESCA003691"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415775_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003691-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10079" checksum="15D6B0ED913FAB20" modified="2013-10-16" version="1">MNYHFYCLWGSMGGDNLLQINFARPTPMQTLFPSPKGRNAHGFSLCNSSVENLSGSNRSGSENISGSLWMASTGISTMVSFSMIIFEFGTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP4</accession>
    <name>T1GJP4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02028415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003694-RA">
        <property type="protein sequence ID" value="MESCA003694-PA"/>
        <property type="gene ID" value="MESCA003694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742968_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7917" checksum="A50A0372ED074701" modified="2013-10-16" version="1">MVLIREICLMTSKSQESVSFECPSLQVLKPQKIQIPLTKFKPNEPTLTNNDAGKTAYGGIRSCKGCRGRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK37</accession>
    <name>T1GK37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003852-RA">
        <property type="protein sequence ID" value="MESCA003852-PA"/>
        <property type="gene ID" value="MESCA003852"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1152862_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003852-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="28058" checksum="3A77C73312DA4332" modified="2013-10-16" version="1">MLLETLNEYIICEILKYLDYEDLSNLREISEDFYDIGKDVFVVVKPNGNFKISLDTGKKTDSNTMSIFKTVFQNDVFKENVERLSIMGLNKGSEILHLLEKFPNFTDLEICFKGNYKEDDDQTDYSVFPKIRNLSIHTYVPYNLALSFTAIEKLEFCNSYSEFLIRQNSETLEEIIINHLKVTGPVYHQDIVDMDFQIKSKLKVFTAIGVRCLLTEEFLKNQPDCEVNYVMCFSVNGSDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK59</accession>
    <name>T1GK59_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK59"/>
    <dbReference type="STRING" id="36166.T1GK59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003875-RA">
        <property type="protein sequence ID" value="MESCA003875-PA"/>
        <property type="gene ID" value="MESCA003875"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_12_2_1"/>
    <dbReference type="OMA" id="IIDWAGG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003875-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="10864" checksum="01391B60B2A0B25E" modified="2013-10-16" version="1">MVGVGVEILSCADGKCSAKFNVSDEQVNMGGGLHGGFTATLVDNITTYALMSKGTHPGVSVDLHVSYLKGAKEGDSVLVDATTVRAGKNLAYIECEPDMKRMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLA7</accession>
    <name>T1GLA7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02026331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02026332">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004306-RA">
        <property type="protein sequence ID" value="MESCA004306-PA"/>
        <property type="gene ID" value="MESCA004306"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031684_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004306-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="30906" checksum="67084C9F616F2936" modified="2013-10-16" version="1">MLRSTLLEEKQFLGKILVCDFHVSEISFKINGHETKTNKWLHTFEWEQIYEKLDDILKKTKELNLIDLDMSSFRIDNYIEVFLKKASVHGKCFPQLLTEPSPPSSQPLEYDPTINGNAGNTLAYTPSNISNSEEAYTPSKIKAEQSVNVSPSVYVPKPISPSKRDYIVSPEFSASTLQYKASKISPTIRSESPKKKKIALKKEETEPRHDRPKRAASTKKEHSKRPSRSDDEGEDTKDIKKKRENVNIKPRIPSVKTDTKSEIERSWQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLG1</accession>
    <name>T1GLG1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hemolymph juvenile hormone binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004363-RA">
        <property type="protein sequence ID" value="MESCA004363-PA"/>
        <property type="gene ID" value="MESCA004363"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069908_2_1_1"/>
    <dbReference type="OMA" id="ILVAHIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF18">
        <property type="entry name" value="BCDNA.GH05536-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004363-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27351" checksum="578E0750DBB2D5CE" modified="2013-10-16" version="1">EIDDLSEEKYPEQPLLKSVNRGACEIGSPSFDSCILGKFREILPKLTNGVPEYNMPVADPYFYRKGIFRYANDGIQGGVLVRNMNIRGIANSRINSIRTNFQDTNNFEVNVDGFLPRLIADGGFKADVKFGGLRLVPKGIFNVTVLNVKAQIRTTGSLVQTPQGQRVQLKTLDADIDVGNAKVSANGIFSDRNLSKYYDLEFDHENMPEITRTGIPATKSQWAPIVIQHINGFFANVPIEQLLTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM29</accession>
    <name>T1GM29_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004597-RA">
        <property type="protein sequence ID" value="MESCA004597-PA"/>
        <property type="gene ID" value="MESCA004597"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_614352_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="446" mass="50192" checksum="395D84CA3EC5AEB7" modified="2013-10-16" version="1">MVGELGIGGVMHKDESSTNTPSPSRPNRNNHNMMNMNDLLGDTNPKISPAPPAGWGMMQPRRYDNYNNNGNNNFNARYVPPSAQGPLPHIVKHNSPPHHPQNNQQNMFHHSSPNNFEQMYKQQQQSAGPPQHLPPPQIPNQGPNFFQELMRQQMWEQRSPTSSGGNNLLKIPNQQQTAAAMGIKNGNESPIFRSMQEKRRIENGNSPYKAYNNNNNGNIANNNFKDDIFLQSPTQWSLGGEQNSFMADLMRQNSRDSFGHVILQRTLIFPLRRILLVNLARFREMLEPRMASIYHHLLETFHQEISNLAEIGCTLPTTSSPHTVDSLQPIVDSKFQYNSPGNESGILSPNKYDLGIGNLSLIDSKDGHINEKLTSLWNNNSPATPTNKLLNSSKDLHHQQLKSANLQVPHSHDDEMGIANGIKEFEKRLESEFGSEYLEDSGISNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMI6</accession>
    <name>T1GMI6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMI6"/>
    <dbReference type="STRING" id="36166.T1GMI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004760-RA">
        <property type="protein sequence ID" value="MESCA004760-PA"/>
        <property type="gene ID" value="MESCA004760"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172744_0_0_1"/>
    <dbReference type="OMA" id="PTVWAGR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF253">
        <property type="entry name" value="DPR-INTERACTING PROTEIN ETA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="27"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004760-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7478" checksum="074F898C305E450F" modified="2013-10-16" version="1">MGAYLCIASNGVPPSVSKRVTLIVHFPPMIAVQNQLIGAVEGRSVTLECQSEAYPKSINYWTRERGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNE5</accession>
    <name>T1GNE5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNE5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005093-RA">
        <property type="protein sequence ID" value="MESCA005093-PA"/>
        <property type="gene ID" value="MESCA005093"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457344_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005093-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10321" checksum="F5070C8FD7171F6B" modified="2013-10-16" version="1">MWFKTGQGGSTMLTKQNRFNGSVWDFETNKSGEDSCKQSDTLQFKLLGIAEFLEIRLNLFVCNFGQFWWVPFGLIVFVDEQCSDAFFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNN3</accession>
    <name>T1GNN3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID 150 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02178454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNN3"/>
    <dbReference type="STRING" id="36166.T1GNN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005191-RA">
        <property type="protein sequence ID" value="MESCA005191-PA"/>
        <property type="gene ID" value="MESCA005191"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002317_0_0_1"/>
    <dbReference type="OMA" id="EQPDYQW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000018">
        <property type="entry name" value="transcription initiation factor TFIID subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000003">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="9">
        <location>
            <begin position="512"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="10">
        <location>
            <begin position="619"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="10">
        <location>
            <begin position="743"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="967"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="972"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005191-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1028" mass="117318" checksum="EFAD2DE6A24EBCA1" modified="2013-10-16" version="1">MLIAHQSVIITGISLENKTIIGLVELTVVPTRENLQFLKLNAKQCTIYKVILNDVCEAEFYYYDHSWTYVEMMLKSRKKSLETFYKSHLKAAEAVDPDLNSGELVIQIPAEGQHLIQEGRGLRISVEFSIENPKGGIHFVLPPDPKNEAGINQSSAHMFTYSYENSTRLWFPCIDSFSDPCTWKLEITVDKHLTAVSCGELVEIVMTSDLRKKTFHYSLTTPVCAPNIGLAVGPFEIYVTPNMHEVTHFCLPQLMPLLKNTVRYLHEAFEFFEETLTTRYPFTCYKQVFVDETDCDIAAYSTMSIASTHLLHSIAIIDQTYISRTAMSKAIAEQFFGCFITTEHCSDSWLAKGIAEYLCGLYSRKCFGNNEYRTWIQQELAEVVKYEQQYGGIILDCSEPPSPLPVTTQGASSNINKQNEPMHYFPIKNLHTMSPKYLEVMRKKAHLVIRMLEHRIGQELLLNVFNKQLKLAENAANTKIGCGLWGQLLISTNIFIKSIFTVTGKDMSVFERNTIELEIRQDAFTQNQKGVRKYNGPLLVQLQELDGTFKHTLQIENTVVKADITCHSKSRRNKKKKIPLVTGEEVDMDLTPMDDSPVLWIRLDPEMILLRDLNIEQPDFQWQFQLKHERDVTAQFEAIKALEKYPTTSTRQSLTTIIESESVFYKVRCQAAYCLSRVANAMVTSWSGPPAMLSIFRKFFGSFSAPHIIKQNNFSNFQLYFLQKAIPSAMSALRTAHVFVHQKSMLVDSLGDTITPVISVVQQGAPITSENLSADTKLILEEVTRLLNLEKHLPSYKYLVSVSCLKVIRKLQKYGHLPSRPNIYRCYAAYGQYIDLRIAAMECLVDFVKVDGKWDDLDHLITILETDPDPFARHALARLLIDNPPFTRATDNVKTNAELDQPQLAKRIWNMMNFDLSHETKIRCDMVDLYYALYGSKSSVDNVSSKTTKFPKHEYKQLFVPATDNKISSSSLKREHDDIRKKDSDENETSLEPSAKKLKPKYDGLVMAKNDISSVIQMIQKLSLTRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNP8</accession>
    <name>T1GNP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNP8"/>
    <dbReference type="STRING" id="36166.T1GNP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005206-RA">
        <property type="protein sequence ID" value="MESCA005206-PA"/>
        <property type="gene ID" value="MESCA005206"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751801_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052231">
        <property type="entry name" value="Rho_GEF_signaling-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845:SF3">
        <property type="entry name" value="PURATROPHIN-1-LIKE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="90"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005206-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17260" checksum="92057611F9405391" modified="2013-10-16" version="1">MADSGLGGCDRCEGVQEKLSRVCSCQSFDETGNLCDKSDEMDEDYETQAKQFMDLHSPIEANAHLQYHASTLELPKMYELSNLDPKIQKQLLLIMREMITTERDYVRSLYYVIENYVQELSEDLFTLSADLFQLIKDILRLNEDSFPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP28</accession>
    <name>T1GP28_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005344-RA">
        <property type="protein sequence ID" value="MESCA005344-PA"/>
        <property type="gene ID" value="MESCA005344"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834054_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005344-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7799" checksum="204394571057A5E9" modified="2013-10-16" version="1">MFLHKPECISKGRMEDGKKRWSDVISLDSRAIGILNWHTTAKNREVWKWNLIEGSSSLDINIMYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP51</accession>
    <name>T1GP51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02026868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP51"/>
    <dbReference type="STRING" id="36166.T1GP51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005368-RA">
        <property type="protein sequence ID" value="MESCA005368-PA"/>
        <property type="gene ID" value="MESCA005368"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278944_0_0_1"/>
    <dbReference type="OMA" id="MRTESAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF60">
        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005368-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="24992" checksum="E5A7C2E5AF6EC96C" modified="2013-10-16" version="1">MSFYERLQNTFYSLYEDLHREYVYFPIQDELVKKYFSHLPITIPSISHMEKNLSTMLLNSHAPLTSTRPTVPGMIFVGGMHIKKIPDPLPADIRDFIENAKDGVIYFSLGSNVKSSEMPAENRQIFLDVFAQMKQRIIWKFEDENIPKLPPNVMVKKWLPQPDILAHPNIKVFITHGGLFGTQEGVYNAVPMLGMPFYCDQHLNRTKLSNQDTLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPR8</accession>
    <name>T1GPR8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPR8"/>
    <dbReference type="STRING" id="36166.T1GPR8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005609-RA">
        <property type="protein sequence ID" value="MESCA005609-PA"/>
        <property type="gene ID" value="MESCA005609"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_538170_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043449">
        <property type="entry name" value="PHF20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15856:SF51">
        <property type="entry name" value="MBD-R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15856">
        <property type="entry name" value="PHD FINGER PROTEIN 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005609-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="507" mass="57888" checksum="67BD978C593C6207" modified="2013-10-16" version="1">GEAANISSSTSHITEQLEATPLKESPPHVSSILPVELMTSCLNVSERDEEALSPLQEGYVYVGSLKVQLVDNLFKCPDENCSKTFRKENHFQIHVKHYHPSLVDKLGACPNIMDLAYKRTFVTPQSIEQPKNQIPNQQFFAKMHLQDQQARLQRRSLANTPSSSTNVTPMQSPIHCPVIHTPEELNSQSLILDTSPTTKATLSREDDLNVNSNAADISASSKKSCERKSSRQRTTRKFVSSSSSKLFDDSFRAGVDSEETRHSFNFTPDSQKSPMTKKPKLQQYLHLLLHLQLSVPIEEPKYIQENGELIKIVRMRQEEIINCVCTYGEEDGLMIQCELCLCWQHGTCHGIEKECQVPEKYICYICRNPNRGRQSKKFIHDQEWMFDGKLPTASYHANNPNSEQKAEFLKKSHTLTGNLIDLKRVLHSLKAKVNIVENKDHPKLYLWSKDWDSPEMKDQKIKEEISQMPEPEAAIDPAECQKTLLKHLKIQKMLLQQKINDIAEQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ38</accession>
    <name>T1GQ38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 10">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 10"/>
    <dbReference type="EMBL" id="CAQQ02390413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ38"/>
    <dbReference type="STRING" id="36166.T1GQ38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005737-RA">
        <property type="protein sequence ID" value="MESCA005737-PA"/>
        <property type="gene ID" value="MESCA005737"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1333275_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052273">
        <property type="entry name" value="PPIase_FKBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP7/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222:SF3">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005737-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="206" mass="22947" checksum="BAEE6763825C538F" modified="2013-10-16" version="1">MCVGEKRKLTIPPELGYGDRGAGNVIPPKATLHFDVELIHIGDAAPPTNVFKEIDDNKDNQLSREEVSEYLKKQMTGAEGQDNEELKKMLNDHDKLVEEIFQHEDKDKNGFISHEEFSGPKHDELLINFLIAKASNKKVIMSNLFQMFKFAVVASCLCLAALASCDELKVDVVSVPEVCEQKSKNGDTLTMHYTGKLTDGTTFDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ54</accession>
    <name>T1GQ54_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Galactokinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GQ54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005755-RA">
        <property type="protein sequence ID" value="MESCA005755-PA"/>
        <property type="gene ID" value="MESCA005755"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123894_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="3">
        <location>
            <begin position="21"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005755-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12423" checksum="EC540E1CF5416D67" modified="2013-10-16" version="1">MASQITKLSSFEDILEQAKTTYKEKFGSFPDIACCAPGRVNLIGEHVDYNDGFVLPMALPMVTVIVGSANRTNSKANIVTCCPEAXXXXXXXXXXXXXXXXXXXXXXXXXMNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD5</accession>
    <name>T1GQD5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Syntaxin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RGS7BP/RGS9BP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02013496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQD5"/>
    <dbReference type="STRING" id="36166.T1GQD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005839-RA">
        <property type="protein sequence ID" value="MESCA005839-PA"/>
        <property type="gene ID" value="MESCA005839"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_203419_0_0_1"/>
    <dbReference type="OMA" id="MAWLIVE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026512">
        <property type="entry name" value="RGS7BP/RGS9BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21029">
        <property type="entry name" value="R-SEVEN BINDING PROTEIN (R7BP) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14523">
        <property type="entry name" value="Syntaxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="Syntaxin N-terminal" evidence="3">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005839-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7692" checksum="F25C0A347F7E1046" modified="2013-10-16" version="1">MAWLIVEINSQVALFRDMLIHVGQSKDCPELREKIRKLRRSCIEACKHTGHLILPQVKRSNFFVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQF7</accession>
    <name>T1GQF7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQF7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005862-RA">
        <property type="protein sequence ID" value="MESCA005862-PA"/>
        <property type="gene ID" value="MESCA005862"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3299935_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005862-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="40" mass="4488" checksum="F9236559FABDC482" modified="2013-10-16" version="1">MNFSILKTLIFCVAIVTASAFNMRRDKRSPFLPDLSAVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQH0</accession>
    <name>T1GQH0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQH0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005877-RA">
        <property type="protein sequence ID" value="MESCA005877-PA"/>
        <property type="gene ID" value="MESCA005877"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944343_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005877-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="7147" checksum="4E25DD950B841C78" modified="2013-10-16" version="1">MSEEEDCVLLDDDKNYKSITTVVYVNILAKIDFEVHISLQMKSIFYWINFYQKLVTILLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQK7</accession>
    <name>T1GQK7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQK7"/>
    <dbReference type="STRING" id="36166.T1GQK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005916-RA">
        <property type="protein sequence ID" value="MESCA005916-PA"/>
        <property type="gene ID" value="MESCA005916"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514291_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005916-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20409" checksum="82F9729BC60052C2" modified="2013-10-16" version="1">DHQLFILNCQGSPYELAYEYEGLEYGICVTSFPAQHCPGSVMFLLQSNQKNILYTGDFRISLSRFEKYSRLKEIKLHEIYLDSTFLTEEYTYFPTQVEKTHPSKTVSDSLANEYIFLNQFLLKYSSELLSSSDNKCAWSSSSQSNMTSVISESRIGLSYSKIILSTVPYSSLWIDSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRA1</accession>
    <name>T1GRA1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02016487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006179-RA">
        <property type="protein sequence ID" value="MESCA006179-PA"/>
        <property type="gene ID" value="MESCA006179"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_509325_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="535" mass="59668" checksum="A6CE72746FD8BC41" modified="2013-10-16" version="1">MVSFLDTDKILKEHIDSMSKEKGPMQPPLDLRNIALKSMDFSSSSSNNHSTENTQKPVRRRFKNPPPLPIPAWADALSKKYQSNDQTGKSQKTPEKFPDLSQDRPETPNSTPSTPKAPSNQKKKKEKEDKEKDVSKEKSLCEPSKEKKKKEPKQKTVPQQTPPAKKIEPETVIRTPTPKSQSQSPVPSKTLPDLLNHSKPGILNPPKKTGKTPGRERKIKEHNHTNPFPQPVFETSSSSVPPSPDDVAEYKSNAFQFSSPLEMDDVHDVISNKEDPWNLDLSGYKENKDLGPIVSPITSQPVSLQSDQEVNKAETVPLDDYKLMFEVVKYQHELIQNLEWNRTQQNLAEIRNQNIPNSWNSFQTPVSNISMPLTNHIPSMNSIPLSASPGPLNRMPIPTISTAPNNINHLQAMPATVPTPLNHLQTISGTAQIPMNHVPVANMTGQPLNSLNMQQMNNMVPMMNGNMNNLNINRVAPPPGLERLNRTLAQNSNQGLFNDGTGNNNNGSSGNALNNDRYNLSAIWSADWKPNNNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRK4</accession>
    <name>T1GRK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRK4"/>
    <dbReference type="STRING" id="36166.T1GRK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006289-RA">
        <property type="protein sequence ID" value="MESCA006289-PA"/>
        <property type="gene ID" value="MESCA006289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307599_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052821">
        <property type="entry name" value="F-box_only_SRC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46432">
        <property type="entry name" value="F-BOX ONLY PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46432:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="211" mass="24354" checksum="80F8C6B7FEB2CA6B" modified="2013-10-16" version="1">LEQVNNQQRPGPANPQLGNNVNGNNINNNNNRADGNAQGLDEDEAANRLQRNLVLRSRDNEPILPRRFDELQEDRFRMAAFSDNRLSRNVNRNEERIRRMEAKMNALRTLRREMNEQKMLKESKRVKTNVFSVYVCDISRALCQTDPHLKWIEYKNYGIIPEAPERLILSTLVSGNGQLILFGGVHKETLTEVTHQVSNSVHFLQAPFSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRR1</accession>
    <name>T1GRR1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRR1"/>
    <dbReference type="STRING" id="36166.T1GRR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006351-RA">
        <property type="protein sequence ID" value="MESCA006351-PA"/>
        <property type="gene ID" value="MESCA006351"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022576_1_0_1"/>
    <dbReference type="OMA" id="ACGNGEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10974">
        <property type="entry name" value="CE4_CDA_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.370:FF:000003">
        <property type="entry name" value="CLUMA_CG003232, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000128">
        <property type="entry name" value="Vermiform, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052740">
        <property type="entry name" value="CE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985:SF1">
        <property type="entry name" value="VERMIFORM, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="60"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006351-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="536" mass="60912" checksum="2452054176EAA869" modified="2013-10-16" version="1">MVSASERVKRQDDDVPDINIEELCKDRPGDEYFRLTTEGDCRDVVRCDDAGENGVTRLASVRCPTGLAFDITRQTLTGKLKCTKSGLKQITCPSGLAFDIEKQTCDWKAKVPNCDQLEKPRKALPNLKTIEPICPDGKLSCGDGECINKELFCNDTPDCKDESDENACSVDDDPNRAPECDSSQCELPDCFCSADDDHLSFNGAINVDNVDLYEEIFNGVRQNPNGCSIKGTFFVSHKYTNYSAVQDLHRRGHEIAAFSITHKDDPNYWTKGKYDDWLAEMAGVRLIVERFANITDNTILGVRAPYLRVGGNKQFEMMADQYFVYDASITASLGRVPIWPYSLYFRMPHRCNGNAHNCPSRSTPIWEMVMNELDRRDDPTFDESLPGCHMVDSCSNIQTGEQFGRLLRHNFNRHYNSNRAPLGLNFHASWLKSKKEFRDELIKFIEEMLTRNDVFFVTNLQVIQWIQKPTEVNSLRDFADWKDKCDVKGQPYCSLPNPCPSTTRELPGETLRLFTCMECPNNYPWILDPTGDGFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSA7</accession>
    <name>T1GSA7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006559-RA">
        <property type="protein sequence ID" value="MESCA006559-PA"/>
        <property type="gene ID" value="MESCA006559"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973006_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="15534" checksum="1A1696643FC47131" modified="2013-10-16" version="1">MTRNFWRQLWLTLSMKLYQIYETVHREERPAFDQLAAQSTSLSCRNWDLETRKLSKIFHRMMVCLEFAKELLNHGRCMKTRIVFWLRMWPAIFARIIHYEGIKHLKVLRKAFTEIYRQVKLGTNKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSK1</accession>
    <name>T1GSK1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSK1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006661-RA">
        <property type="protein sequence ID" value="MESCA006661-PA"/>
        <property type="gene ID" value="MESCA006661"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2910792_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7295" checksum="6E65608B91CA729E" modified="2013-10-16" version="1">MELFQYTRKAEFEYTIETFDDEMKDLFNKRGTGLKSSEMLQYLKDAEFGMSQRGKVTLLIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSP5</accession>
    <name>T1GSP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSP5"/>
    <dbReference type="STRING" id="36166.T1GSP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006705-RA">
        <property type="protein sequence ID" value="MESCA006705-PA"/>
        <property type="gene ID" value="MESCA006705"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1857566_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF271">
        <property type="entry name" value="DPR-INTERACTING PROTEIN GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="14"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006705-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="14716" checksum="F786B8CD3E65382C" modified="2013-10-16" version="1">MVRAPSQLLGTPLGSDVHLKCQIEASPMPVSYWLKASQTKQSSSHSSTSSSSSSSSSTGYGGFGGISQSNGGEVFNHQQTEMLLDGPKYGISETHQGYKGTMILTVRNFSQTDVGTYYCVSTNSLGRAEGTLRLYGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTC3</accession>
    <name>T1GTC3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006951-RA">
        <property type="protein sequence ID" value="MESCA006951-PA"/>
        <property type="gene ID" value="MESCA006951"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707606_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006951-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8549" checksum="5FAE868741D39348" modified="2013-10-16" version="1">MTTKFWSEAVNAACYIYNRTPSRGVKGFRIPEEVYTKKKPNLKNFRLTYSRDEEVHIKSFCDDDFGNAFSESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTH2</accession>
    <name>T1GTH2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTH2"/>
    <dbReference type="STRING" id="36166.T1GTH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007004-RA">
        <property type="protein sequence ID" value="MESCA007004-PA"/>
        <property type="gene ID" value="MESCA007004"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512520_0_0_1"/>
    <dbReference type="OMA" id="MSACKAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1590:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.270.10">
        <property type="entry name" value="Myosin, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007004-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="532" mass="61758" checksum="EEB41E9169F06335" modified="2013-10-16" version="1">MKTDFRGVADFAIVHYAGRVDYSAAKWLMKNMDPLNENVVSLLQASQDPFVVNIWKDAEIVGMFRTVSHLYKEQLAKLMDTLRNTNPNFVRCIIPNHEKRAGKIDAPLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYELLTPNVIPKGFMDGKKACERMIQALELDSNLYRVGQSKIFFRAGVLAHLEEERDYKITDLIVNFQAFCRGFLARRNYQKRLQQLNAIRIIQRNCAAYLKLKNWQWWRLYTKVKPLLEVTKQEEKLVQKEDELKQVREKLEVLAKSTQEYEKKYQVAMDEKTALAEQLQAEIELCAEAEESRSRLMARKQELEDMMQELEARIEEEEEHALKHSKRLQAQVKEALREAEDAKAAKEELSALSKEAERKVKTLEAEVLQLTEDLASSERARRAAETERDELAEEISSNANKGSLMIDEKRRLEARITTLEEELEEEQSNSEILLDRTRKSQLAIEQLTTELATEKSNSQKSENARLLLERQNKELKAKLAEIETAQRTKIKATISALESKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTK2</accession>
    <name>T1GTK2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007036-RA">
        <property type="protein sequence ID" value="MESCA007036-PA"/>
        <property type="gene ID" value="MESCA007036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796885_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7797" checksum="582FA270A997CF3E" modified="2013-10-16" version="1">MAKFLALHMLTIQSKLPIFTKITNTASNQTTSYGEFQSSLPFLILFALQTESYMEASSCQRWEVYILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTR9</accession>
    <name>T1GTR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTR9"/>
    <dbReference type="STRING" id="36166.T1GTR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007110-RA">
        <property type="protein sequence ID" value="MESCA007110-PA"/>
        <property type="gene ID" value="MESCA007110"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_9_1"/>
    <dbReference type="OMA" id="LYTKRKX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007110-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="24885" checksum="7040575114472B09" modified="2013-10-16" version="1">LPKFRGINFKVNGGEKIGIVGRTGAGKSSLTLALFRIIEAAGGKITIDGIDISILGLHALRSRLTIIPQDPVLFSGSLRRNLDPFETKKDEEIWKALELAHLKTFAKTLTAGLNHEISEGGENLSVGQRQLVCLARALLRKTKVLILDEATAAIDLETDELIQNTIRTEFKDCTVLTIAHRLNTIMDSDRVIVLDKGEISEYASPSDLLNNEDSIFYSMAKDANIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTT3</accession>
    <name>T1GTT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTT3"/>
    <dbReference type="STRING" id="36166.T1GTT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007125-RA">
        <property type="protein sequence ID" value="MESCA007125-PA"/>
        <property type="gene ID" value="MESCA007125"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1528928_0_0_1"/>
    <dbReference type="OMA" id="SRYSAMW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF448">
        <property type="entry name" value="FI20146P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="11"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007125-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="19662" checksum="045F3ABA66712060" modified="2013-10-16" version="1">KVTNQGSGGGDKTIVGERGVSLSGGQRARISLARAVYREADIYLFDDPLSAVDTHVGKHLFEQCIKGHLKGKVVILVTHQLQFLQDVDKIFIVEKGKVTNQGSYEYLRESGLDFAKMLSESGEKKDGTQTVKEHKRQNSESSLNSVESVKEDNYQQVQENQEKGSIGMALYKKYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTX4</accession>
    <name>T1GTX4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GTX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007172-RA">
        <property type="protein sequence ID" value="MESCA007172-PA"/>
        <property type="gene ID" value="MESCA007172"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798647_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15999" checksum="3098F154E7F1A2AB" modified="2013-10-16" version="1">MSDEFQTIRGCLQGDALSWSFFNILLEMVMKSAKIERGNIIYNKSGQILAYADDIDAIERTPSEVIDKILAIEKAENSVGLNVDGSKTKLMISXXVGLDVNDSKTKLMISSRNQQRHNNLGSNITIGHHNIEIVKLGIGFLSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUJ9</accession>
    <name>T1GUJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM4 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02005771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUJ9"/>
    <dbReference type="STRING" id="36166.T1GUJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007413-RA">
        <property type="protein sequence ID" value="MESCA007413-PA"/>
        <property type="gene ID" value="MESCA007413"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044783_2_1_1"/>
    <dbReference type="OMA" id="PTPSFQF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000392">
        <property type="entry name" value="Protein-lysine N-methyltransferase Dere_GG24471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03188">
        <property type="entry name" value="Methyltr_EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026635">
        <property type="entry name" value="Efm4/METTL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843:SF5">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="6">
        <location>
            <begin position="59"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007413-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24264" checksum="AC97760393829957" modified="2013-10-16" version="1">MSIEELSGSELGTKEFWERSYDREIRNYKNNGDVGEIWFDQDSQDRVISWILKQDDIDASHSVIDLGCGNGMLLLELGAEGFTKLLGVDYSPKAVELANNIAKDNKVNIKYSVLDLLDTEAAQEFGEFDIVHDKGTYDAISLNPDCPKEKREAYIKSVNHLLKKEGVFIITSCNWTEEELVAGFEGTFKKTGHIPTPTFKFGGKVGSAVTSLIFKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUP6</accession>
    <name>T1GUP6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02392188">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUP6"/>
    <dbReference type="STRING" id="36166.T1GUP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007464-RA">
        <property type="protein sequence ID" value="MESCA007464-PA"/>
        <property type="gene ID" value="MESCA007464"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_4_0_1"/>
    <dbReference type="OMA" id="FHAHTIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000078">
        <property type="entry name" value="Seryl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="120"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007464-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="321" mass="36775" checksum="4950F567A732555F" modified="2013-10-16" version="1">MDNLEKYANLRKSVNNIPEIRKLLTSLQESTDSETSKLLSDQLKQELSRLPNETHPDVMDLHEPVILKTYNKFPEFPSPPHDFDDIAKKLNLIRSSHLGNFCGHKSYYFLGALAELEHALVRYALDNIRPKGFKYITVPDVLPPEIIQGCGMQTDGERNQVYKLESGLCLSGTSEMALAGFFHNKILEEDELPIKLTAASRCFRAETSGTHDEKGIYRVHQFTKVEMFSVCTETQSDALLEEFKNTEIEMFEKIGIHFKLLDMPPNELGAPAYRKYDIESWMPGRNFWGEISSCSNCTDYQAQRLNIKYRQRVGKLNLSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUR9</accession>
    <name>T1GUR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SLC26A/SulP transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUR9"/>
    <dbReference type="STRING" id="36166.T1GUR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007489-RA">
        <property type="protein sequence ID" value="MESCA007489-PA"/>
        <property type="gene ID" value="MESCA007489"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123891_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="6">
        <location>
            <begin position="28"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007489-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12534" checksum="830F1BE927368224" modified="2013-10-16" version="1">MSFCVMPKWSIARNRVMSRICFPVTFRNLKFKHGLYSSFLGCLVYIFLGSSKDVPIGPTAMSGLLVFQAAQGNWQKAVLLTFLTGLIEIGMGFLQLGFLLDFVSGPVSAGFTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUZ3</accession>
    <name>T1GUZ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUZ3"/>
    <dbReference type="STRING" id="36166.T1GUZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007566-RA">
        <property type="protein sequence ID" value="MESCA007566-PA"/>
        <property type="gene ID" value="MESCA007566"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1604594_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007566-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="166" mass="18808" checksum="86F369AF7EE89D9D" modified="2013-10-16" version="1">MKLDRVMLRRNRGFGHLFFCEKECISRAGPGRFDTVLCYGRYTLLDESLRDYIGFFKEQELAVICAAVHGMGLLSRGMKGILPTHPAPQEIKDCVKRAVDICEEADVELGKIAMYYAAQYKEPSIFLVGMQKLPLLEMNLKAVTEGLTPKEGQVLETLKETKPKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW20</accession>
    <name>T1GW20_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">28S ribosomal protein S18a, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW20"/>
    <dbReference type="STRING" id="36166.T1GW20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007988-RA">
        <property type="protein sequence ID" value="MESCA007988-PA"/>
        <property type="gene ID" value="MESCA007988"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107628_1_0_1"/>
    <dbReference type="OMA" id="ACQTKFC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF66">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007988-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17149" checksum="7CFA5E4D3567377D" modified="2013-10-16" version="1">MSLLKVVAALNRNIVSQQRTVTTSAVLGIKQIEIQKQDESVVIQGVTSESPRQSLMLKEACQPKFCPECTLGLEIKHTDVLILSQYVDGCMLPRRITGLCRRQQKKMGTLVTMAQKAGLMPNLAPANSKKDPKQRFGWRKYNKYFYESSIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW41</accession>
    <name>T1GW41_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008012-RA">
        <property type="protein sequence ID" value="MESCA008012-PA"/>
        <property type="gene ID" value="MESCA008012"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266798_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11769" checksum="EB615D9DE2823F4C" modified="2025-10-08" version="2">MATPPLLPHLQTPPLLSVSNGYHQHHNHHHNHQFDYKRQASSRDKHYPNHMVSSSFCNNIINENESLLTGNNSDDSPVSVLANDRSYVEIIEIPVNKKKSSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW87</accession>
    <name>T1GW87_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008059-RA">
        <property type="protein sequence ID" value="MESCA008059-PA"/>
        <property type="gene ID" value="MESCA008059"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161247_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008059-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12439" checksum="1138184964C841B7" modified="2013-10-16" version="1">MNSLQIKFNFQCNYNNHHRCYWFLVASFLLVNLTAIGQGSEFPERECCDLTTTTSTISPLSIPPIQATEKTITTSSASVQPISVGRSVVIIKTFLGLVLTPRLWKAFNKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWI6</accession>
    <name>T1GWI6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWI6"/>
    <dbReference type="STRING" id="36166.T1GWI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008163-RA">
        <property type="protein sequence ID" value="MESCA008163-PA"/>
        <property type="gene ID" value="MESCA008163"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2229846_0_0_1"/>
    <dbReference type="OMA" id="CELNNME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="36"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12209" checksum="FB3165C81E9F8F80" modified="2013-10-16" version="1">MSGNYHHNKYNQRCSGGDGHEVEEQHRNGYGMEMPPRPMDQVAIPAMCFFCFDVLYCELNKLDMPIDPSFTNDAYPLFVTWKIGRDKRLRGCIGTFSAMHLREGIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWQ7</accession>
    <name>T1GWQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWQ7"/>
    <dbReference type="STRING" id="36166.T1GWQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008236-RA">
        <property type="protein sequence ID" value="MESCA008236-PA"/>
        <property type="gene ID" value="MESCA008236"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_422957_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000191">
        <property type="entry name" value="Multiple epidermal growth factor-like domains 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000202">
        <property type="entry name" value="Multiple epidermal growth factor-like domains 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF433">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="309"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="355"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="407"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008236-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="650" mass="72678" checksum="B634BE9D8540521B" modified="2013-10-16" version="1">MSRVQKLSSLHDRSLCGWCSLTSLCLPRTANESIFCVNDQNWNYLTIQAEQCSNCSDYINCKDCLESSECEWWVDDAKCERVGRAALAVKSIDKCPVPCKERQGCSECLDQGGRCVWCESSQKCFSFSAYTSEFQFGMCREWYDQVVPRINSGPGQNTTHLASEKKPQCKSCSYFNDCASCLKALNCGWCYDSENPIKGKCVAGDFSNPVQTCNSVLNTTHLTAEWAYAQCPDVDECNLNIHHCHKEAKCTNTHGSYTCQCRKGYVGDGRISCIRTCYETCIKGKCSNSPNYECICDLGWTGKDCSIDCGCNNHSHCEKVGVCESCQDWTEGEHCERCRPGSYGNATTSEGCSPCECNGHGNQNLGVCDSRTGHCFCQDNTDGLKCEKCQKNYYGDPKNGGTCYYQCESRGYFTGLEKKSFGSFHSFKNPWSDHEVHECLWAVSPANVSGSVLITLEIDWSSMNVNCEENAAYVYDGQPDLLGQYPQSQLVGVICEPYTVQRPIETRSGHMTVYFKQTGKTKDGFNAHYSIKSCEKSFKEKCICPGTKIGPKCKESVCPEECNYKQQHGYCDMDYGRCICYNGWGGNGCETRIQSHQLISQTLFNTANLYDQLEHLRITIPRFGHSLSADKRGSLWMFGGYSLPNGPLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU7</accession>
    <name>T1GWU7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02070873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWU7"/>
    <dbReference type="STRING" id="36166.T1GWU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008278-RA">
        <property type="protein sequence ID" value="MESCA008278-PA"/>
        <property type="gene ID" value="MESCA008278"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631247_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008278-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="17707" checksum="EAFB6202A6F6DDAA" modified="2013-10-16" version="1">QDHPPLVLEGLDAGVYYEIFVAALNAHGKGGPSPRLVFRTKYQENVDPLPIQSYNMTKCCHASGLLPQCMPLCTLDIKMSDIHELGATCQAQMGTGNIPGPVEDVHVTSVTNNSISLAWVPPDVSGNNSSSANIADNYTDFVIQYGKVNNMTMYETVVKLEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU8</accession>
    <name>T1GWU8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWU8"/>
    <dbReference type="STRING" id="36166.T1GWU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008280-RA">
        <property type="protein sequence ID" value="MESCA008280-PA"/>
        <property type="gene ID" value="MESCA008280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984120_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14505" checksum="B510D0C5C987E642" modified="2013-10-16" version="1">MISYLDEVIPNPKQRSQKWKSTSVRIEKWGIEGEYCSGEYFANNFCGYVSFEETCKTLPDKCLSLEIGPTGQLFKIVEQILPNGVHFALGQRENPKAVELFLNTLKEARQHGLVFDQQRLDTILEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXU8</accession>
    <name>T1GXU8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXU8"/>
    <dbReference type="STRING" id="36166.T1GXU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008657-RA">
        <property type="protein sequence ID" value="MESCA008657-PA"/>
        <property type="gene ID" value="MESCA008657"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392221_0_0_1"/>
    <dbReference type="OMA" id="YDCGAFS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF42">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008657-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10652" checksum="F3640443B35F9532" modified="2013-10-16" version="1">MDYYNFQNYDCGAFSSDSHSPNSQSPKSAKPGRLKVNARERDRTQSVNTAFTTLRTLIPTEPCDRKLSKIEILRLAKSYIEHLDAVVVTETVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYK8</accession>
    <name>T1GYK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYK8"/>
    <dbReference type="STRING" id="36166.T1GYK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008940-RA">
        <property type="protein sequence ID" value="MESCA008940-PA"/>
        <property type="gene ID" value="MESCA008940"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1210992_0_0_1"/>
    <dbReference type="OMA" id="YSSTRMF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008332">
        <property type="term" value="F:low voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070509">
        <property type="term" value="P:calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000008">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF230">
        <property type="entry name" value="CA[2+]-CHANNEL PROTEIN ALPHA[[1]] SUBUNIT T, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="62"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008940-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="229" mass="26482" checksum="CF11B3CC910B929E" modified="2013-10-16" version="1">MFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRKMHEPPYYTNYSSTRMFVHNVCTSKYFDLAIAAVIGLNVVTMALEYYMMPVALEYALKIFNYFFTAVFILEAVMKLIALGFHLYMKDKWNQLDVVIVILSIVGIVLEELETKIIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSPKIPVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ41</accession>
    <name>T1GZ41_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149236">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009133-RA">
        <property type="protein sequence ID" value="MESCA009133-PA"/>
        <property type="gene ID" value="MESCA009133"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3034724_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009133-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="55" mass="6022" checksum="74E2AEB0459C40A1" modified="2013-10-16" version="1">MMKSTPPKKRLRRLRKSLKKLKKRRKFLVFSTFSDGGGGGGGGGGGGRNDFLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ77</accession>
    <name>T1GZ77_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02118594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ77"/>
    <dbReference type="STRING" id="36166.T1GZ77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009175-RA">
        <property type="protein sequence ID" value="MESCA009175-PA"/>
        <property type="gene ID" value="MESCA009175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867440_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF216">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15267" checksum="DDF6D3D65E684F2C" modified="2013-10-16" version="1">MGTTIRIPKPLYGEDGPAITISRSGVYEVGEYDIVDVLKVKTMLLIIQMLEDDNAVVSGCHEIIDMSKATMGHFLQMTPALMKKMSSFADEAIPIRQKGAHFINTPVGFEQIFNLFRSFLSPPPPPPSIVIFKHLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZC1</accession>
    <name>T1GZC1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZC1"/>
    <dbReference type="STRING" id="36166.T1GZC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009220-RA">
        <property type="protein sequence ID" value="MESCA009220-PA"/>
        <property type="gene ID" value="MESCA009220"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349053_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF45">
        <property type="entry name" value="CYTOCHROME P450 6G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009220-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="11457" checksum="335F225CA6392C49" modified="2013-10-16" version="1">MVQRWYNHQSANNQPFVGIHVFFRHGILVRDPELIKQVMVKDFNKFSNRSSGADIHNDTLASANIFFAKNPEWRTIRQKLTPVFTSKVRSEEVERFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZD3</accession>
    <name>T1GZD3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZD3"/>
    <dbReference type="STRING" id="36166.T1GZD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009232-RA">
        <property type="protein sequence ID" value="MESCA009232-PA"/>
        <property type="gene ID" value="MESCA009232"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309177_0_0_1"/>
    <dbReference type="OMA" id="WVERNLI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009232-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11598" checksum="675DA848BDD22A5C" modified="2013-10-16" version="1">MVDRYIYERGCLRAGEDWLDNNMFSVTFFFLLLTISKVEATDISKIIYEKGCVTAGEEWVERNLIIISTICILILFAQLLAICFAQNLRVDILAQKNKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZZ4</accession>
    <name>T1GZZ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Epoxide hydrolase</fullName>
            <ecNumber evidence="6">3.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes juvenile hormone hydrolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol</text>
            <dbReference type="Rhea" id="RHEA:55764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:139161"/>
            <dbReference type="ChEBI" id="CHEBI:139164"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cis-stilbene oxide + H2O = (1R,2R)-hydrobenzoin</text>
            <dbReference type="Rhea" id="RHEA:23900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:50004"/>
            <dbReference type="ChEBI" id="CHEBI:50014"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.9" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02187075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZZ4"/>
    <dbReference type="STRING" id="36166.T1GZZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009456-RA">
        <property type="protein sequence ID" value="MESCA009456-PA"/>
        <property type="gene ID" value="MESCA009456"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_3_0_1"/>
    <dbReference type="OMA" id="TRIYYEF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009456-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="348" mass="39797" checksum="9FB210AE6463A7B0" modified="2013-10-16" version="1">ILDLKDHLSRPLKLAAPLEGVGFEYGINTNYLRTLVKYWRDDYLPRWTDRQNYLNSFPQYKTKIQGLDIHFIHIKPQQKSKDILPLILLHGWPGSVREYYGLISELISGNHEKYDFELIVPSLPGFGWSQASSKKGLSPSAMAVIFKNLMKRLGHEKFLIQGGDFGAIIGSHLATLYPDSVIGVHSNMCGVNGPSSVFKWMFASVFPTWFVTSEQISFHFPISSYLSKVIEETGHLHIHSTKPDTIGTVFSRNPVGLLAYFVEKFATWTNPEFKKFEDGNLEKKFKLDDLFDNIMIYYLTNSITTSQRIYAESFAKTEMALQLDNIPTRFLMDVLGSNMRCFIVWIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H012</accession>
    <name>T1H012_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H012"/>
    <dbReference type="STRING" id="36166.T1H012"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009476-RA">
        <property type="protein sequence ID" value="MESCA009476-PA"/>
        <property type="gene ID" value="MESCA009476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280585_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="3"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11940" checksum="CDB8D66CDD19BB96" modified="2013-10-16" version="1">MDGVTHGDEIQYLFDYKEAGYFEQCSDDGKMMDFITGLFTRFAATGNPNEPGKKEIWKPANKKQFSMAFINTPDENGNIKTGTVDPYMDRYRIWDDLFHFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H052</accession>
    <name>T1H052_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H052"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009520-RA">
        <property type="protein sequence ID" value="MESCA009520-PA"/>
        <property type="gene ID" value="MESCA009520"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188794_0_0_1"/>
    <dbReference type="OMA" id="HEMNRRV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009520-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="6966" checksum="F3911E182AFC4752" modified="2013-10-16" version="1">MQRIGGNVDTKIAGLYDLGETLGSGHFAVVKLAKHVFTGEKVAVKVVDKTKLDEISRAHLFQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2021-06-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0E8</accession>
    <name>T1H0E8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA009624-RA">
        <property type="protein sequence ID" value="MESCA009624-PA"/>
        <property type="gene ID" value="MESCA009624"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3435225_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009624-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="14" mass="1565" checksum="DD3C63C35CC55E29" modified="2013-10-16" version="1">MIKVASFLPTSRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0N3</accession>
    <name>T1H0N3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0N3"/>
    <dbReference type="STRING" id="36166.T1H0N3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009714-RA">
        <property type="protein sequence ID" value="MESCA009714-PA"/>
        <property type="gene ID" value="MESCA009714"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_16_0_1"/>
    <dbReference type="OMA" id="FEETGQX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="23"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009714-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8038" checksum="A9FC1F6BFF6C51E9" modified="2013-10-16" version="1">MGGLLSYFRGFIGNREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVEQVTYKNLRFQVWDLGGQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H107</accession>
    <name>T1H107_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H107"/>
    <dbReference type="STRING" id="36166.T1H107"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009858-RA">
        <property type="protein sequence ID" value="MESCA009858-PA"/>
        <property type="gene ID" value="MESCA009858"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433179_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009858-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10334" checksum="0B1734B288FE4285" modified="2013-10-16" version="1">MGLANRVVASGTALGQAMNLAQSIAKFPQGALNHDRNSLYTAMYEAQTFNQSIQNEIMYTSSEIMEELKEGVKKFNDEWVDQNWYTFGLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H125</accession>
    <name>T1H125_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02060049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02060050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H125"/>
    <dbReference type="STRING" id="36166.T1H125"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009876-RA">
        <property type="protein sequence ID" value="MESCA009876-PA"/>
        <property type="gene ID" value="MESCA009876"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170777_0_0_1"/>
    <dbReference type="OMA" id="MAECITG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="3">
        <location>
            <begin position="17"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009876-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="13412" checksum="CB507633BC22893D" modified="2013-10-16" version="1">MLTRHIHLARRRLFLLHTKRTGWVMRNVNDCESISGHMYRMSMMTFLIDDSLNLDRLKCMQLALVHDLAESIVGDITPFCGISKEQKRNMEMKAMEDICKLIEPRGEKIMELFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1E3</accession>
    <name>T1H1E3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1E3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010002-RA">
        <property type="protein sequence ID" value="MESCA010002-PA"/>
        <property type="gene ID" value="MESCA010002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10620" checksum="13FB56DD8752C6A7" modified="2013-10-16" version="1">MSYLRYIFIAEREHTDYFQLENHVNCNVYVKRDCVFDKDERCSLGGDNLLQVLRQRHCRKTRNRLWGLYTCVRTAVVEMKGRRGHKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1U1</accession>
    <name>T1H1U1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APH-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1U1"/>
    <dbReference type="STRING" id="36166.T1H1U1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010161-RA">
        <property type="protein sequence ID" value="MESCA010161-PA"/>
        <property type="gene ID" value="MESCA010161"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086389_1_1_1"/>
    <dbReference type="OMA" id="PLSCCTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009294">
        <property type="entry name" value="Aph-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12889">
        <property type="entry name" value="GAMMA-SECRETASE SUBUNIT APH-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06105">
        <property type="entry name" value="Aph-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010161-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="18107" checksum="C0A2AED3F59E3176" modified="2013-10-16" version="1">MTVAEFFGCTFLVFGPVLSMFILTLANNNDPIRIIIFIASSFFWLLSLLFSSLIWFLLNPFFDALIVGVIISIASQEGARYFVYELLRRTETGLLEFTDNNSIVQNKHILSYVSGLGFGTSSGIFILINVLADMVIDFFIYKLHISSLEGKESIPYFLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H252</accession>
    <name>T1H252_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H252"/>
    <dbReference type="STRING" id="36166.T1H252"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010284-RA">
        <property type="protein sequence ID" value="MESCA010284-PA"/>
        <property type="gene ID" value="MESCA010284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662792_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18811" checksum="16BB091C770CBD0E" modified="2013-10-16" version="1">MMHNWKKFMTLLHERTLNGFEYDVYVGVSGYTKVPLELKHKMDFRPKRLIYYPQRIPKYIWAYLNTTSSSKEAERLIVIGVNSIFVTKGSFEDYSLCKDVCDKFKWLEPLSITRHMAAFGYMYCCHLTNNIRKLLEGFSNLHCPETMFIDPECGMYDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H266</accession>
    <name>T1H266_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H266"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010299-RA">
        <property type="protein sequence ID" value="MESCA010299-PA"/>
        <property type="gene ID" value="MESCA010299"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1995219_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588688" evidence="1">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010299-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14633" checksum="2B00F8D77A3EDEF8" modified="2013-10-16" version="1">MKVLIFIVFILQYICICLAYDKDITFQDFKDCQQEYVSEGNGSQSWADFKKIIAIDTLRNNYKHSDEILRTRFYYQGPRDLSVMLSDINRDYKVGDSYFHTILGANCNSSSAVIYIDRDWHACTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2F4</accession>
    <name>T1H2F4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02013931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2F4"/>
    <dbReference type="STRING" id="36166.T1H2F4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010399-RA">
        <property type="protein sequence ID" value="MESCA010399-PA"/>
        <property type="gene ID" value="MESCA010399"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1471781_0_0_1"/>
    <dbReference type="OMA" id="MISLACC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF33">
        <property type="entry name" value="AT22075P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010399-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="184" mass="19950" checksum="DBC9437483B72A5B" modified="2013-10-16" version="1">KPNNLKGIVIGLGVVCTNQCSGAFSILNYSTAILEKSEGFQLDISTSTIVLGLLQILGTYSATLTVDRFGRKIVLLISASGTAVGLLTAGVFDYLTYKFEFLKEYSWVLILSLASVIYLANMGVCTVCYVVLVEIIPIKVRAVATSLCLLISSSLSFTQLKVFPILLESIGFYGLMWTFGTLMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3C8</accession>
    <name>T1H3C8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3C8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010752-RA">
        <property type="protein sequence ID" value="MESCA010752-PA"/>
        <property type="gene ID" value="MESCA010752"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984119_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010752-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14675" checksum="9D05FD363CBF4EFF" modified="2013-10-16" version="1">MVCKNILKSVGEVMKNHQKTDRLRLHLISLEVHQQLAAMTQTVPKAQSLLPRLVHQKSQKKRRKTKTGIENGVSARRRKNEIRRSPNLHKSLVPVDKVSRPRQQFQCLQRLLHQIILRLLKATTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3D4</accession>
    <name>T1H3D4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3D4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010758-RA">
        <property type="protein sequence ID" value="MESCA010758-PA"/>
        <property type="gene ID" value="MESCA010758"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870144_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010758-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="6941" checksum="B45B0B6353E14892" modified="2013-10-16" version="1">MIFGAICIKDHQHSLHPGKSSAELLKAVGCLQSAANQNLECRNDAKRMESFAQSKKGAKENGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H429</accession>
    <name>T1H429_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H429"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011028-RA">
        <property type="protein sequence ID" value="MESCA011028-PA"/>
        <property type="gene ID" value="MESCA011028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500508_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9671" checksum="7F10BE9BDE105E16" modified="2013-10-16" version="1">MRRKSIDGIPAELLKAAGATFNYVFYCLQTSGLAKRPQRNRNTFNSGKEKEEVKNQSTKKSIFPYRQAVGALMYLICGTRPDLAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H432</accession>
    <name>T1H432_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H432"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011032-RA">
        <property type="protein sequence ID" value="MESCA011032-PA"/>
        <property type="gene ID" value="MESCA011032"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798653_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011032-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="15796" checksum="20BBCF87B99E6A0A" modified="2013-10-16" version="1">MREALAMRLELKESRVALLVVKELCIAFENSGVPLVDYVEKVELEVAGCLKSTSGIAAKVNICKPNTDSIGVTDKLISIYVDCQTAQKTLVASIRISSEIVKTCKKSLKELSSANSLRLCWIVGNVEIEGNDTVDTCQRYARKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H450</accession>
    <name>T1H450_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the muscleblind family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H450"/>
    <dbReference type="STRING" id="36166.T1H450"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011051-RA">
        <property type="protein sequence ID" value="MESCA011051-PA"/>
        <property type="gene ID" value="MESCA011051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574629_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19073" checksum="9CCAA575772DF2F1" modified="2013-10-16" version="1">DKVEICDQRVAVCRDHANGQCRRKQCKYYHIPVILPPADIMATIINNNNSTLTTTTPPSTSSTISTTATIESVAISPQTHKQSSPTSATSTTTSTTFWPYDYFLKNLTGPYRELNCYSFLSLDTASLSPPSIISNSPIIYNSSVEEVFQNQHHPRKTMQLQLHHKRKEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4F0</accession>
    <name>T1H4F0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377851">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4F0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011159-RA">
        <property type="protein sequence ID" value="MESCA011159-PA"/>
        <property type="gene ID" value="MESCA011159"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186926_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011159-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12501" checksum="5665501C30889718" modified="2013-10-16" version="1">MERERINLIPLNGTDGLRHDGINLHLASTPINFLVYWIRDTNELDTAAFRRYVIATCDFAILEPLDSSIKDMENKKNLGLGVFNTSLKDINVLLTNWIIEDTNYNVHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4F1</accession>
    <name>T1H4F1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4F1"/>
    <dbReference type="STRING" id="36166.T1H4F1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011160-RA">
        <property type="protein sequence ID" value="MESCA011160-PA"/>
        <property type="gene ID" value="MESCA011160"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906657_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011160-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7108" checksum="FE2BC37FFE59C295" modified="2013-10-16" version="1">MLVLNEPICPDQVANVSRDCMTAYIECEIMKKMKTTSSDQKLSATWKDIYLVRENIVISARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H538</accession>
    <name>T1H538_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02088729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H538"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011412-RA">
        <property type="protein sequence ID" value="MESCA011412-PA"/>
        <property type="gene ID" value="MESCA011412"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349059_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005228">
        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000058">
        <property type="entry name" value="Potassium sodium-activated channel subfamily T member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="12">
        <location>
            <begin position="30"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011412-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="10981" checksum="D68F5BFEDEB83B08" modified="2013-10-16" version="1">MFNDLHRAMQKSQSALSQQLTILSATLVCLVFTSVCGIQHFQRAGHRHLNLFQSTYYVVVTFSTVGYGDFVPDIWPSQLYMVIMICVALIVLPTQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5B9</accession>
    <name>T1H5B9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5B9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011496-RA">
        <property type="protein sequence ID" value="MESCA011496-PA"/>
        <property type="gene ID" value="MESCA011496"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3299932_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011496-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="40" mass="4680" checksum="67032309322BA867" modified="2013-10-16" version="1">MLLFFPPPFVHLLVDNLPVATRTRDPNTQELKFEHGYRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5S5</accession>
    <name>T1H5S5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5S5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012058-RA">
        <property type="protein sequence ID" value="MESCA012058-PA"/>
        <property type="gene ID" value="MESCA012058"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009579_6_5_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF233">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="167"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012058-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="306" mass="34630" checksum="770840D3BDB8428A" modified="2013-10-16" version="1">EIPEIPSYIRHISLITCITIMLLGFIGNTLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAEYKYVEYIDGSDVPVCLTQASSTWSIGYFLMTISIFFFIPLFILILLYGIIAKNLVSKNGAMMKIRPNKPELSLKARRQVVLMLGAVVLSFFICLLPFRAFTLWIIFSSQESILSLGIEKYYGILYFSRIMVYLNSAINPILYNLMSSKFRKGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5V3</accession>
    <name>T1H5V3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5V3"/>
    <dbReference type="STRING" id="36166.T1H5V3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012086-RA">
        <property type="protein sequence ID" value="MESCA012086-PA"/>
        <property type="gene ID" value="MESCA012086"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190931_0_0_1"/>
    <dbReference type="OMA" id="ELICEWR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF15">
        <property type="entry name" value="BEATEN PATH IIIA, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012086-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="8060" checksum="B2ADED2D182EA3BE" modified="2013-10-16" version="1">QISGLRITEMRIPNLIMRNKSATLGCKFEMEGESLYSVKWYKDGFEFYRYVPRDMPPAQVFPLPGIDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H762</accession>
    <name>T1H762_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in mitochondrial morphogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM11 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H762"/>
    <dbReference type="STRING" id="36166.T1H762"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012600-RA">
        <property type="protein sequence ID" value="MESCA012600-PA"/>
        <property type="gene ID" value="MESCA012600"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1997758_0_0_1"/>
    <dbReference type="OMA" id="CHYQVDY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026120">
        <property type="entry name" value="TMEM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15099">
        <property type="entry name" value="PROTEIN PM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15099:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 11, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14972">
        <property type="entry name" value="Mito_morph_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12587" checksum="03CD4164C64B3194" modified="2013-10-16" version="1">TTKEPTYSVIREVYDNGVAFEKFEQLLDKALEAKVDYIIIEPTRLGDETGRWIYVGNCLHKTAVISGIASIISSLIWQNRLLVSSPICVISIFCTGLYTVSWNYDPCCQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7I6</accession>
    <name>T1H7I6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S27</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7I6"/>
    <dbReference type="STRING" id="36166.T1H7I6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012723-RA">
        <property type="protein sequence ID" value="MESCA012723-PA"/>
        <property type="gene ID" value="MESCA012723"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130128_3_0_1"/>
    <dbReference type="OMA" id="YQDEWET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.100:FF:000001">
        <property type="entry name" value="40S ribosomal protein S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012723-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9155" checksum="6FFC9B3186CA6F59" modified="2013-10-16" version="1">LARDLLHPSPIEEKRKHKLKRLVQHPNSYFMDVKCPGCYKITTVFSHAQGSVVCAGCSTILCQSTGGRAKLTEGCSFRRKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Z6</accession>
    <name>T1G9Z6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Virilizer N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vir family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1G9Z6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000038-RA">
        <property type="protein sequence ID" value="MESCA000038-PA"/>
        <property type="gene ID" value="MESCA000038"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_245782_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031801">
        <property type="entry name" value="VIR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026736">
        <property type="entry name" value="Virilizer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23185">
        <property type="entry name" value="PROTEIN VIRILIZER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23185:SF0">
        <property type="entry name" value="PROTEIN VIRILIZER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15912">
        <property type="entry name" value="VIR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Virilizer N-terminal" evidence="14">
        <location>
            <begin position="691"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="617"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="975"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1430"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="987"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1048"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1439"/>
            <end position="1451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000038-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1564" mass="177569" checksum="BB1016E12EE4F3F5" modified="2013-10-16" version="1">MSLQGLHCCQIAIPTLEILREMMIYVIQCRDSDFKDITAIEQLLKTYYLMYYFPEHSMSYEPQENYEEETLTKSLWTQMIREVFKNILSGGLWKYMPGLQLLAELLPLPLPIPSLTALGEAEQQHLLVERKLWSFHLHPESNLIAQLIRTICPTSFPQLLNFLTRVCLQLADLAPNMTLLVCKTVIDLIIAEPLTNAVHLARLLNFLDQLTCYASIKISALSIFSGKLYEAFCNILCFTQEPVEAMLRCQLSVHRILEHFFDSEISLMSISEPENSDESVKELNLACALPPKELIPGIIEAILKNLLETDIKSHVSLAASRNLIILSEHDITFYYLKKTLITRKIEYQSWITKVLDLHESGEHKGTLKSLVTLSMSIAELASIVGIENTEEQKDRPIKRIRDILQTSEDLTNKEALMQDIEDLMGKIETHLENPPTDLDHDILSDPPYLPQAESIVTQYSKRPLTVSLETIDDDRTMRISYWTEPLSLNDMSEDMIEQMEKVSCDITEVISSCLPSETNLASDCKRILHLSASPQSQKERLTTAPCFRKRRVDLDPIPQSRPDKKMFISNLSRRGFQRPPPSRGDLFRSRPPNTSRPPSLHVDDFLALETHGVTPTCPTGYNKIPPIIRGGGRGRGRGSRISNSGFRTNSPSYAPPTHFRTATSEPVTHFSSDSHYSGSHFGVRSKDETEEELLFFDTFSHEIHEDINLDLVQFPQPVYITEVRIIPLGARVQADFPGGVRLGATNPSKFGIEFFVNDLGKPGVSTFEKLGKLEYNQNDCINLECDRIPTDGLVLRGWYSTITLAVYGILNTTLNDPIASPPPSEIVEEPEVVPEIVPTIKEIVPFQEPPKENYFERNMRLKKALSPSLGIYKPEITTVLSISHRITGTILSGYTSFLGIGSLVCPHDMSHCIPSMLDSFNMSSTTVTILKFLFAFPFAYHFCAGARHLIWDTGMFLNDKMPPLKVEPEEIPEEKRIHVHRKSRSPLNEREYKWSRSPEYHRSHLQPPFSRPQSPDESDPYHSPAKSIRLDEDEGATDPPLEPISDGSDLEFDDEEPYEGEEEQEDDTLELDSAIKVFDPYKHPIGKYEVNFETVLQKDIEEFHLISKKFEKQTKISSSVEFMASLSVEKENFVILTEQLLTHFCHVGQNLKLSNAFFEIIFLNNIENQNLILNMLNVSLDYECAKSQPQPLHKIRHIKIGIRIAEILGGSAFVLETKSLNVFNRLFALYSEDYMALPIKLLILKAISSILDSEKGLRCLHPMKFPVAARPKFAMKSILKKINFFDTLESLKAYVNETFILKNGEDRSEEIEKCIKEVSSILTTDSVSLQQFKRHLPVVKFSMSNDPAAKKGFLSMVQNYFKINSFGECVLLILTNLSIGMKSTLIVAVHDLIEIMMKTHVGIDYLTDDCFETTQLMVNCLLGTDAVTENIPGESKDEPMEEDDEKMDPEEPISPRSHNLGLQLAFKVQTRYHIDAITFEKNNVDELVDHLHSLYSQTCSHPGRDFTVEVLGLNGSLTPFLDLIEKERKIQVQRQQDSPGVKYKSPVLSYSVDILDCCFDIAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA30</accession>
    <name>T1GA30_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02017173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA30"/>
    <dbReference type="STRING" id="36166.T1GA30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000075-RA">
        <property type="protein sequence ID" value="MESCA000075-PA"/>
        <property type="gene ID" value="MESCA000075"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_666625_0_0_1"/>
    <dbReference type="OMA" id="QTMFAGR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023252">
        <property type="entry name" value="Aurora_borealis_protein"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02038">
        <property type="entry name" value="AURORABORA"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000075-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="413" mass="46277" checksum="C3556771E88A6B08" modified="2013-10-16" version="1">MSSTAAVAAESVGAEKQSIRCQAQRVILAHISGNTPVSKPGRRIRDSSAQTILSFPPDLPPEIEAVLKPYFSYHENQQQDIDKEEVYVDYEARDALLRRKLFDINDYQEIEEPAIYEYDHFSPPPKSPELDTKPFQDSLDKSSFGSLSPISKLERTVSPSKDSPIQTNKRTVSPSKDSPIQTNSAINEDCSIGKRKSSFLNSSFTDERETPTQSRHIHLRNASLSSNSYRNSPNDGCSSEGTPGTVINGSLYRSTPGLLSTRRSSSPCFESNVSIDRSVNRKISQLVMNSAKRPKSEITDIFKEEDETEDEELQLSQFSTSIESPGKFKNADHADTPRGSQQKRRVRAKNLSQSFTLHSEDEDFEKVDKENLNSKVNYVYSSSSTSTFYRTDSGFNELTQSSTASQEIIEYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAV8</accession>
    <name>T1GAV8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000373-RA">
        <property type="protein sequence ID" value="MESCA000373-PA"/>
        <property type="gene ID" value="MESCA000373"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624844_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000373-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8644" checksum="99F6AA29385E134E" modified="2013-10-16" version="1">MCSVIREDPQHFRRMSSRHQQRPPPCKSNTAMKPSETVAIQTSIVQNKSSAISSANIASSTIPSWDMESKNIGRTTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAZ6</accession>
    <name>T1GAZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02070177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAZ6"/>
    <dbReference type="STRING" id="36166.T1GAZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000415-RA">
        <property type="protein sequence ID" value="MESCA000415-PA"/>
        <property type="gene ID" value="MESCA000415"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_12_1_1"/>
    <dbReference type="OMA" id="GWHNKKR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="12"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="HIT" evidence="10">
        <location>
            <begin position="282"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="8">
        <location>
            <begin position="375"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="5">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="370"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="7">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000415-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="428" mass="49017" checksum="CA9DDC540D7B2E5F" modified="2013-10-16" version="1">MFAPPEMMSTLKRVAIAQMRSTSNKLENNKSVEKLFQLAKEKDANFLFLPECCDFVGENRKQTLELSEPLENGETVKFYRNLAKEHKMWLSAGGVHEAILNEKGEKTDKIMNAHVVINDLGDIVAVYRKLHMFDVNTPAFRFRESEVVEAGKKIVPPIETPIGQLGLQICYDMRFAEVSTLLRKAGAEILTARAIENQCYVFAPAQIGFHNEKRQSFGRAMIVEPWGSIVAECNETDLDVVTADIKSEAIEKVRTNMPCFEHRRKDIYGLASAAYDCNQFPCDRSFAEHNISKDTIFYETQHCYAFTNLRCVVEGHVLVSTKRKVKRLQELNCDETSDMFQTVCKIQKVLENYYKTTSTTVTVQDGPEAGQTVKHVHFHVMPRKEGDFKHNDQIYLELQKNDLDETKNRNLEDMVKEAQLYRSLMAKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBN5</accession>
    <name>T1GBN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galactosyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBN5"/>
    <dbReference type="STRING" id="36166.T1GBN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000671-RA">
        <property type="protein sequence ID" value="MESCA000671-PA"/>
        <property type="gene ID" value="MESCA000671"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044391_8_1_1"/>
    <dbReference type="OMA" id="VRTQRTW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033842">
        <property type="term" value="F:N-acetyl-beta-glucosaminyl-derivative 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF57">
        <property type="entry name" value="BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000671-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12765" checksum="F485E05D8AD11FA6" modified="2013-10-16" version="1">MRREQFSKVNGFSNSFWGWGGEDDDMSNRIRHANYFISRYPINISRYRMLNHKKEKANPKRYDRLNSGVTKYTTDGLNSIKYEIYKYNKFPLFTWFLVELKMKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBQ9</accession>
    <name>T1GBQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBQ9"/>
    <dbReference type="STRING" id="36166.T1GBQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000696-RA">
        <property type="protein sequence ID" value="MESCA000696-PA"/>
        <property type="gene ID" value="MESCA000696"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062640_3_0_1"/>
    <dbReference type="OMA" id="LSINAEX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000696-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="21926" checksum="D9F90ABA2C83C75B" modified="2013-10-16" version="1">MLIFKEDIVLNGYTCIVPSLGVGNVSQLSTDLIISTLKLKQVATGFHKALIPIFGPSAFQHEPDSFTNALNVYIDSSKKLLVFQIRSPLIGKHTKDFLNNLVEFLKKSQVSKINSGEEPVFGDGWGQKLLKICSASELPAVMFYKYSAEGDNTLDAAMLVMEVNKYVPLFEDSKQPNLIRPTSWEMLFGNEPIGTMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBS3</accession>
    <name>T1GBS3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000711-RA">
        <property type="protein sequence ID" value="MESCA000711-PA"/>
        <property type="gene ID" value="MESCA000711"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017866_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588172" evidence="1">
        <location>
            <begin position="25"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13426" checksum="013070BBCF801563" modified="2013-10-16" version="1">MYLKLKLFAFLALYFTAIVGEVQKCHFGDLKCIETTASSLVKLGDGSPEMNLPDLHNFNFNGLRFLRNGDSSPIEADLRIIESNITGISGMKASNAKGFGKDLKGPHSITFKSKSTTLLGLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC25</accession>
    <name>T1GC25_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02067245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC25"/>
    <dbReference type="STRING" id="36166.T1GC25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000820-RA">
        <property type="protein sequence ID" value="MESCA000820-PA"/>
        <property type="gene ID" value="MESCA000820"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1580298_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="1">
        <location>
            <begin position="27"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000820-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="19326" checksum="701C2561C04E15C9" modified="2013-10-16" version="1">MMTNIYFKIREKTIPFAAKGPRHSRRNFQGEESKIILLSLVRNNEENKIGFLSLKNRICVALSRAKEGLFVVGAMDFFGKNNSIWKEIIEKLSEKAFIGECLPLVCQRHNTSIEAKTSQDFKKLKFGGCFEPCDFEFPCGHVCSLVCHWDEFDHCSNCNCRKTGSGDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC84</accession>
    <name>T1GC84_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126533">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126534">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000888-RA">
        <property type="protein sequence ID" value="MESCA000888-PA"/>
        <property type="gene ID" value="MESCA000888"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944346_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000888-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="7252" checksum="974F74F6A6410CFB" modified="2013-10-16" version="1">MNVWYITNVIQKDQCTELVHIDSCFLKIHWIFRTYLNDLQYQKTFINRDPSNAFLISSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCL5</accession>
    <name>T1GCL5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185590">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCL5"/>
    <dbReference type="STRING" id="36166.T1GCL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001031-RA">
        <property type="protein sequence ID" value="MESCA001031-PA"/>
        <property type="gene ID" value="MESCA001031"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="OMA" id="CIEIRDI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000042">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602402-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001031-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43394" checksum="E9AC1A9547127AA7" modified="2013-10-16" version="1">DLYQKYKNVSSVAGLYFFFQPVAFVTDLDIAQKILVTDFHNFADRGIFNNEQGDPLSAHLFALDGPRWRYIRNKLSPTFTSGKMKNMFPIVVEVANNFQNVYKKLLKNESIIEIKDLNARFTTDVIGTCAFGLDCNSLLDPNADFRRNCGNFFTKRRHHPLIEGFIMSFPKLAEKLNMKFIPQQVSDFFIKSVEETVAYREAHNIQRNDFMSMLINLKNHSTIDENGKEIEGITMGQLAAQAFVFFLAGNSEIQDTLRSEIKDFLNADELTYEIVNKMPYLEQIVMETLRKYSVVPDLSRVALNDYKVPNTNFVIEKGTRVMIPADAIHHDPSIYPEPETFNPERFNLNEIKKRHPLTWLPFGGGPRNCIGMRFGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCM7</accession>
    <name>T1GCM7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001045-RA">
        <property type="protein sequence ID" value="MESCA001045-PA"/>
        <property type="gene ID" value="MESCA001045"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796892_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001045-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7887" checksum="BAACD6AFA009EEE0" modified="2013-10-16" version="1">MDFKYAELTRKKGLSTTKELIKLLNYNPGYCEHNLSNGITINGYGLVCAKTYWTWFQNVNHVLNQTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCU6</accession>
    <name>T1GCU6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 11">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 11"/>
    <dbReference type="EMBL" id="CAQQ02085297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCU6"/>
    <dbReference type="STRING" id="36166.T1GCU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001119-RA">
        <property type="protein sequence ID" value="MESCA001119-PA"/>
        <property type="gene ID" value="MESCA001119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009629_2_1_1"/>
    <dbReference type="OMA" id="WFDQWFG"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="87"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="410" mass="46179" checksum="97163FD99B121ACE" modified="2013-10-16" version="1">MPVVLGGGIGGLSAAYYLSRKHISPIKLFEKSNRYGGWIKSENHRDKGYIFECGPRTLRTNGPAAANSLQLIQDLELNDAVVPVSRNTHKLDDESIFDFTERRFGKEVAQYLISPMICGICAGDAKKISVKFLMKEFFEAEQKHGGVFKGLVLNGLFGKKVPKESFLDAEVEIQKRAKEDQWNMILMKNGLEMIPKRLVTYLNNNNVSMDLNSECRKLTFYSDKSLAVHMTGKEKELTTDHIISALPSYKLASCVEEQHPSLAFQLRSIEYVDVAVVNLQYATMDLLDIDAFGFLVPPSEQRPILGVIFDSCCSDMSGNTVLTVMMGGEWFQDYFGSKPDPAYLLNVAKSQVKDILNISMEPKNVKLNILKKCIPQYTLGHYERVEAIRKYIDNYNLPLTLVGALMMELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCU7</accession>
    <name>T1GCU7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyadenylate-binding protein</fullName>
            <shortName evidence="7">PABP</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02044907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCU7"/>
    <dbReference type="STRING" id="36166.T1GCU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001120-RA">
        <property type="protein sequence ID" value="MESCA001120-PA"/>
        <property type="gene ID" value="MESCA001120"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_22_2_1"/>
    <dbReference type="OMA" id="QQPGFMP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000004">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000049">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001395">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="28"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="121"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="225"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="9">
        <location>
            <begin position="492"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001120-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="575" mass="63234" checksum="E7DEA77A82183126" modified="2013-10-16" version="1">MNFDLIKNRPIRIMWSQRDPSLRRSGVGNVFIKNLNKSIDNKAIFDTFSAFGNILSCKVAQDEKGNSKGYGFVHFETEEAANMSIEKVNGMLLQDKKVYVGKFIPRKEREKELGEKAKMFNNVYVKNFGEDMDDEKLREMFEPFGKITSHKVMTKDDGKSKGFGFVAYESTDAAEAACSALNGKELSDGKTMYVGRAQKKAERQKELKRKFDELKKKRQDSFQGVNLYVKNLDDTVDDEVLRKHFSRFGTITSAKVMMDEEGRSKGFGFVCFNLAEGATRAVTELNGTILGSKPLYVALAQRKEDRKAHLAAQYMRHRVQPMTQIFPPNTTGGFFVPTLPQPQRFYGPQVTQIRTTPRWAPQAQVRQASAQGGAAVPAGSNFPNAAIAGTQYRPGAGAAGGVRGPQVQVSHVAGGASGQPMRSAVRAITGQQAGAGGAGAAGVPMAGTSVSAAGGNQRQTNYKYTMSVRNQPIPQVQTPQQPVQQIQKSGNQEPLLATLLANAPPQDQKQILGERLFPLIERMYAQLAGKITGMLLEIENSELLHMIEDQTALKAKVEEAISVLQVHHKQPSKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE97</accession>
    <name>T1GE97_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02027701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE97"/>
    <dbReference type="STRING" id="36166.T1GE97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001655-RA">
        <property type="protein sequence ID" value="MESCA001655-PA"/>
        <property type="gene ID" value="MESCA001655"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019238_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47412">
        <property type="entry name" value="FI01434P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47412:SF1">
        <property type="entry name" value="FI01434P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001655-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="21014" checksum="7A3B7876DB828F21" modified="2013-10-16" version="1">MFLDMVSRNEGYMQRKAPRVFPLHIFEVGGNIPLPRSKTELQGMLRNNKAIPFHKKDCVNCNNVPKAKEWVASIETGNLGVFLITKRKGSYVHWEPIYIGTNTDPHYDERLGDTKVQNYALCLLDYEFHLLNNAFLIHKSDGSLRKYDFKAKMLMEKENLLIKKTITAEVDLLFGSREGCYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEX1</accession>
    <name>T1GEX1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02114771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001894-RA">
        <property type="protein sequence ID" value="MESCA001894-PA"/>
        <property type="gene ID" value="MESCA001894"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906661_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001894-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7537" checksum="EB6975E4741D8A62" modified="2013-10-16" version="1">MKVNFEHWNSTSKTNALHTKLQTTPNNFINHLVDFPISLQKLQFTAFFRPQSFENYRIFRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFH6</accession>
    <name>T1GFH6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002118-RA">
        <property type="protein sequence ID" value="MESCA002118-PA"/>
        <property type="gene ID" value="MESCA002118"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239693_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002118-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11656" checksum="051DD80FF49DC4F2" modified="2013-10-16" version="1">MTLNDTWSVKASGTTTENFRTIGGFQKDDALSCSFSKHRQLNLQQNSNFRIRQRSSSQKYPNILEENSKGTSKGSLLEIAGLATRRVPGLRMGGTHDGAIRTRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFK0</accession>
    <name>T1GFK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02395051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002144-RA">
        <property type="protein sequence ID" value="MESCA002144-PA"/>
        <property type="gene ID" value="MESCA002144"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1697534_0_0_1"/>
    <dbReference type="OMA" id="SETRCHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="18357" checksum="47F865CF3ADA1AB9" modified="2013-10-16" version="1">MSSSDEAEEFVSLTVQANQRRIETVKIPKEEFDKALKLLNEPDTAKPKLKYCHSYIHNTRNRHKDVRAGKLLQIMSDLRRHLMDRNWVEIMRLLKVARPGKKEENYLFPILVRYCMMCLVHHRGFNLLDSFLNGVTLCASDEDKEDLFFEITRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFM7</accession>
    <name>T1GFM7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002171-RA">
        <property type="protein sequence ID" value="MESCA002171-PA"/>
        <property type="gene ID" value="MESCA002171"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725075_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002171-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8515" checksum="BC6E4242CE23098F" modified="2013-10-16" version="1">MIYKEDTEEGFLSNTIQSNSPLREKPFSLSFRNGTEPPRSYRKFLQEFLVQFWGGIGHIHYFQFVGKTVIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFY8</accession>
    <name>T1GFY8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-polymerase II-associated protein 3-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFY8"/>
    <dbReference type="STRING" id="36166.T1GFY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002291-RA">
        <property type="protein sequence ID" value="MESCA002291-PA"/>
        <property type="gene ID" value="MESCA002291"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415786_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025986">
        <property type="entry name" value="RPAP3-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13877">
        <property type="entry name" value="RPAP3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA-polymerase II-associated protein 3-like C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002291-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10581" checksum="B773965ABD37E997" modified="2013-10-16" version="1">MPPKSTAEFHANWKDLSLENKFKYLKSIEIQKLTNILGTGFESVHLTEILSILSTFYVEEKLEPQKVLREICKHENIGILALFMDGKDKDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGA2</accession>
    <name>T1GGA2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02070756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002415-RA">
        <property type="protein sequence ID" value="MESCA002415-PA"/>
        <property type="gene ID" value="MESCA002415"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623131_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002415-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="18048" checksum="494B7345FB0DEE5B" modified="2013-10-16" version="1">NNVLNSSSTTTVPHPEQTSVNKPQIPNSNLIDSTTAVLSNLNLFDSNNSFLVQSFHPMLKKLEAERGQQLPDLINGIDGYQNHLNNGLNGSASSSSLNGGLGHLNGINNLNNLVNLQNQLNYNQPEDWEQVLKLAMLRNNKQMEERNNLLNMMGNGLGNSFSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGC0</accession>
    <name>T1GGC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="MESCA002434-PA"/>
        <property type="gene ID" value="MESCA002434"/>
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        <property type="entry name" value="CEPT76/DRC7_peptidase-like_dom"/>
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        <property type="entry name" value="COILED-COIL AND C2 DOMAIN-CONTAINING PROTEIN 2A"/>
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            <end position="136"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="541" mass="62202" checksum="52C7C172C5653F31" modified="2013-10-16" version="1">MNIVRASGIPNRLLQTNVYDRSGSNASSYFLAQNVKTTNVKPFVSLSYKKNLSRSTTAEGSNPIWNETLDLEVVESPVDLKDDIRIEIFDEYVENLINEDYSRSMEVYQRIYANWLGEYRLPISTLLKHKMIHGVFEINIPKLLVGYTIHNDHVEIPIENSLDTSASCLLWFTISISPTIEIPETNVFCLDCTESEDLRKHIKNGHQKRFVDPLICTEEGKRICITRLLESVPLPIDVAENLVTISKRFVSLISLVKPTFNPMSGFDGIWLNNRRILEGNICSPKDLGILLSCYFINIGVNCWVVLGNGYPQYEVAYILYKSQANSIDFSLIDPCTGKTYSTNDVICPLLQIKAVFNQSNFYSNIQRETRIAFTNFDFKDSSCWMSLFSRRGYAPTGGFQEEDYIYRDPLKAAEIQKNIERKIMKKINSWRSMRKTVWNRGFHSSMFNILRNLEADTTYDLPPENYLERIQGDFPGYKIFGFTLNFPYSNLSAISDRIRSTGIHLNMHKEVEFATAVYIHSFQIISCRCGFSSYQLFLIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGU7</accession>
    <name>T1GGU7_MEGSC</name>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
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            <taxon>Platypezoidea</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="TolB, C-terminal domain"/>
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        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
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            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="36771" checksum="844EC122AFDC44CF" modified="2013-10-16" version="1">PFSTFVFSWELSSIGIHNFTLLHSKTFVLRVFLCILPSKSLNNTVRIPTLVESTWPENYFSLLPKIFPYKQLHSISENQKGDCSLMHQALQTELDDLYRLWVLDEGNDKCPPKIFIFDLLRDNRKVATINCENLFQKKLISMELDPYSACHSRYEMYITVKDEKNEVPGLTTRINPTYITFLGVRKIGQFYTSDRNGNLYYGNNKMYNQTENIESIVIRKLGVLLGEPKSLVLDLMGMLYYIVDKFGIVVRVTPYKGVLKAEEHEILYFSQSEAPLTQVIFGSMGSVWAVTSNFVAHKDHCNRILYHFMLQSNTSEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGX2</accession>
    <name>T1GGX2_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="MESCA002653-PA"/>
        <property type="gene ID" value="MESCA002653"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673911_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002653-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8711" checksum="B1FBA8DB452A8D5C" modified="2013-10-16" version="1">MYLLKAICLQSFPFTAKLFPSTSRFFFIFFLLDFITPYQLQILFGSLDSPSRSSFQSDSVHYAVLMIGEPSFVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGX7</accession>
    <name>T1GGX7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NUCB1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="MESCA002658-PA"/>
        <property type="gene ID" value="MESCA002658"/>
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    <dbReference type="HOGENOM" id="CLU_031153_3_0_1"/>
    <dbReference type="OMA" id="QETDTNH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
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        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057576">
        <property type="entry name" value="NUCB1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040250">
        <property type="entry name" value="Nucleobindin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237">
        <property type="entry name" value="NUCLEOBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237:SF20">
        <property type="entry name" value="NUCLEOBINDIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25434">
        <property type="entry name" value="NUCB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
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    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588291" description="NUCB1-like N-terminal domain-containing protein" evidence="13">
        <location>
            <begin position="19"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="NUCB1-like N-terminal" evidence="14">
        <location>
            <begin position="24"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="323"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="504" mass="59455" checksum="4441ABE9A4C85AF1" modified="2013-10-16" version="1">MRLIGYLGIIACIGLAVSLPVTPKKEEKKEEHPSEDLSSVLEYERYLKEVVEALESDPDFRKKLENAPEADIRSGKIAQELEYVGHNVRSKLDEIKRNEIDRLRKLVKKESFEGCSNGPSFGSHQQHTFEIDDLHKLIKKTSSDLAEADKKRRDEFKQYELQKEFEKNALEKEMDEESKKRYEEELKQKKEKHNKHDKLHHPGNKAQLEEVWEKQDHMDPEDFDPKSFFMMHDIDSNGHWDESEVKALFVKELDKVYQSGIPEDDMHGLISFQEFLDQTKREEFQKDPEWETVDNQPQFTHEEYLEFERKRQEEIQKLIAQGAMPPHPQMPYGYHANGQPNHPNLPPPHYQQHPGVPVHLNQNQVYEHVPQGQQYQQVPQQQFVPQQQHQQGQIHQQVPQYQQQQQQYQQQTQQVPQQQYQQQQQQPQYQQQAQPQQQQAQAPQQQVPQQQVPQQQAQAPQQQVQQQQASQQQVPQQQAQAAKQQVPQQQQNQGQQNINNNQIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH40</accession>
    <name>T1GH40_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002724-RA">
        <property type="protein sequence ID" value="MESCA002724-PA"/>
        <property type="gene ID" value="MESCA002724"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280602_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002724-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11770" checksum="2FD3E16D86F915D0" modified="2013-10-16" version="1">MSSLLAPINADFDLPPLHRFSTGIGRKPEEGLLFYYTKEKNAEFLPTNKLNVRDAVFLLHKGKECRISYTCKQIVRNAVFLLYPGEEFRISSHIQTLKLLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH48</accession>
    <name>T1GH48_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GOST seven transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02021829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH48"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002733-RA">
        <property type="protein sequence ID" value="MESCA002733-PA"/>
        <property type="gene ID" value="MESCA002733"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020277_2_0_1"/>
    <dbReference type="OMA" id="ASXIGET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF2">
        <property type="entry name" value="RE59932P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="7">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002733-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="20648" checksum="FCE4305A482EC34B" modified="2013-10-16" version="1">MVVIPLQVLANIAEIIIAESEEGDVEHGTWREIFILVDLLCCGAILFPVVWSIRHLHEASATDGKAAINLKKLKLFRQFYIMIVCYIYFTRIIVYLLRITVAFQYAWLDEMFHEMATYVFFVLTGYKFRPLPQHPYFQVDSDDDDEVDILTKSGLTANLHNTKASNRTQNPTTTVIENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI31</accession>
    <name>T1GI31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02200194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02200195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI31"/>
    <dbReference type="STRING" id="36166.T1GI31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003098-RA">
        <property type="protein sequence ID" value="MESCA003098-PA"/>
        <property type="gene ID" value="MESCA003098"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1697546_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043537">
        <property type="entry name" value="Tiam1/Tiam2/Sif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46001:SF3">
        <property type="entry name" value="PROTEIN STILL LIFE, ISOFORM SIF TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46001">
        <property type="entry name" value="TIAM (MAMMALIAN TUMOR INVASION AND METASTASIS FACTOR) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003098-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17557" checksum="E829E067FFCDD1B7" modified="2013-10-16" version="1">MWGFSVEAELIENSDRQDELCCYVSRVEDKSVAMQNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQQLSMMMRSSRTEAPDQLGIMRVTDDIESLVCPPPPTDKPIMSEEMISELIVPAPGWRMSVVYIMSIETFHRIGMPNKKLQNVLVEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIN2</accession>
    <name>T1GIN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sec23/Sec24 helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02121377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIN2"/>
    <dbReference type="STRING" id="36166.T1GIN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003315-RA">
        <property type="protein sequence ID" value="MESCA003315-PA"/>
        <property type="gene ID" value="MESCA003315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_455829_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF39">
        <property type="entry name" value="SECRETORY 24AB, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="10">
        <location>
            <begin position="280"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="8">
        <location>
            <begin position="510"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="67904" checksum="7C8115EF3A9FBE87" modified="2013-10-16" version="1">MSLGAQPTQQFQQSLQNGQQKFNQNNQQFQHKQQQQPQIQNFSNATNNLPPQNFQSNLGIHAMNKNTPSIPGLPHMPQPISSYQQPTQQPNMHHSQQPINQQSFVNNNAFQPNKISPIQYPQSQLQNPPQLQHPPQQIQNPIYSNAAPTGIKQMQPPPPTFNGKTNLTPMQYQSQVMPTLNQPQLQQQQMPQIGVTQQGFNRLWGQDTVDLMQSRHILCPTSLEKPKIVLNHAFHESVNSGISKHSGGCIYHFPLYQKTKVEMVESFKRCFRRYLTRKIGFEAVMRIRCTRGLQVHTFHGNFFVRSTDLLSLPNVNPDAGYGMQISYDETLTDVKTICFQAALLYTNSEGERRIRVHTLCLPVSTSLSDILHSADSTAIVGLLSKMAVDRSLSSNMSDARDAFINATIDILSAFRTAQNLPSSQSGQLMAPMNLAMLPLYILALLKHTAFRVGTSTRLDDRVHSMDLMKTLPLDQLIKYIYPEFYNIDSLFIKQASTKENDNEGDESYIMPNRLQLSAEFLDSRSMFLLDCGNLIIIYVGLNVPSHILKNVLGISATAELPDYKYELAELKTTENENLWKFISDLNEDKPYDSTIQIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIQ7</accession>
    <name>T1GIQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleoside phosphorylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02009289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIQ7"/>
    <dbReference type="STRING" id="36166.T1GIQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003342-RA">
        <property type="protein sequence ID" value="MESCA003342-PA"/>
        <property type="gene ID" value="MESCA003342"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1512331_0_0_1"/>
    <dbReference type="OMA" id="ICHIPME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="5">
        <location>
            <begin position="6"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003342-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="19583" checksum="0274AEB3F9CD2746" modified="2013-10-16" version="1">MVFEVKVGIIGGSGLDDPDILENRIESAIETPYGKPSDVIIQGEIKGVKCALLARHGRKHTIMPTNVNYRANIWALKSIGCTHLIVSTATGSLQEQFQPGQLVVPSDFIDRTTKRAQSFYDGGEQSPVGVCHLPMYPAFHEKTRQVLIDTARELNIDVHTKATIVTIEGPRFSSKLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ11</accession>
    <name>T1GJ11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003450-RA">
        <property type="protein sequence ID" value="MESCA003450-PA"/>
        <property type="gene ID" value="MESCA003450"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1911905_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003450-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="14866" checksum="23504BB27ECCD61B" modified="2013-10-16" version="1">AERLATGRNENEQSSELHSSSESAYDLHFSSEPDCDMPLSQQHKQREKSQKRANIHEQSSELHSSFESGSHVGPKIKRNMTKPKTWPTQVSPRYPNIHEQSSELHSSSESGSLAHVGPKIKSISEYILGTVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ83</accession>
    <name>T1GJ83_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ83"/>
    <dbReference type="STRING" id="36166.T1GJ83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003527-RA">
        <property type="protein sequence ID" value="MESCA003527-PA"/>
        <property type="gene ID" value="MESCA003527"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1708360_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153:SF38">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153">
        <property type="entry name" value="UBX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUB" evidence="2">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003527-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14243" checksum="F5AF3911BF4F3F94" modified="2013-10-16" version="1">MSHEAKAAAAAALARVQKKDSKEFNTSLAAIKAQAKRELEAEQKLKEDLEKTKLEDKPESSNKKDYSCNGVFFRCPLVSEEVLPSKEWKDKVEGCVETLTKYIENIINHPDEEKYLKIRMSNRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJI7</accession>
    <name>T1GJI7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitotic-spindle organizing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MOZART1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02163042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJI7"/>
    <dbReference type="STRING" id="36166.T1GJI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003636-RA">
        <property type="protein sequence ID" value="MESCA003636-PA"/>
        <property type="gene ID" value="MESCA003636"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673933_0_0_1"/>
    <dbReference type="OMA" id="IQIRNEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031021">
        <property type="term" value="C:interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033566">
        <property type="term" value="P:gamma-tubulin complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051415">
        <property type="term" value="P:microtubule nucleation by interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022214">
        <property type="entry name" value="MZT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520:SF2">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12554">
        <property type="entry name" value="MOZART1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003636-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8406" checksum="7620CB303AB2E436" modified="2013-10-16" version="1">MTQGNEKDDAYLRIQQSQVIRTSIQGISQLLNTGLDSYSLELCMKLLEAGIHPQALADVVNEINREIGKNEVINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJJ4</accession>
    <name>T1GJJ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390446">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390448">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJJ4"/>
    <dbReference type="STRING" id="36166.T1GJJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003643-RA">
        <property type="protein sequence ID" value="MESCA003643-PA"/>
        <property type="gene ID" value="MESCA003643"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_336599_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056881">
        <property type="entry name" value="Mug62_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF32">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24919">
        <property type="entry name" value="Mug62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Meiotically up-regulated gene 62 protein-like alpha-beta" evidence="2">
        <location>
            <begin position="768"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003643-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="847" mass="91889" checksum="1370C11CB4286B55" modified="2025-10-08" version="2">MFDFTLGAVSKTLTSSELHKRAEKIAALLQEKGKIEPGNHVALIFPPGLDLICAFYGCLYLGAVPITIRPPHPQNLITTLPTVKMIVDVSKSVIVLSIQSIIKLLKSREAATSIDPKGWPQILDIDDHPKRKLAVEASPTLDSTAYLDFSVSTCGRLSGVTITNSLKLACELYPSRHVALCLDPYCGLGFAMWTLIGVYSGHHSILIAPYEVEANPSLWLSTLSQYRVRDTFCSYGRNIDLRCVRTCVVVAEERPRVQLTQQFCKLFQALGLNTRCVSTSFGCRVNPAICVQGASSAESAQVYVDMRALRNNRVALVERGAPNSLCLIESGKLLPGVKVIIANPDTKGHCGDSHLGEIWNQFVAGPNVLNMTQPLTNDMCTQLAAISAEIVSKSNFRGCTPYIPESYANNNPTKSTHSSGNSGISSLEKVSKCCESNSQVQMRVKKTSSSSSSSNIETLSNLPPSAILAAVKNLHSQIKSEIGNDSKLSQQLNSNQQNSNQLQSNRHTEDLVAMKDVLLVVKTDIDFSNFAIFVFESKGLRRDAICPCASSSEVFTVSIRRPGRSGAGGGFTQSASGRGVLSMAALSHGVVRVDSEDSLTSLTLQDCGQVMPAAQMVVVRSDGPPILCKTDQVGEICVTSGATGSSYYGLDGMTNSTFKVQPLIEDPTEKDPNNTKLLSDELYVRSGLLGFLGPGGLVFICGSRDGLMTVTGRKHNADDIIATVLAVEPMRFIFRGRIAVFSIKVLRDERICVIAEQRPDCSEEESFQWISRVLQAVDSIHQVGIYCLALVPPNHLPKTPLGGIHLCEARRRFLEGTLHPANVLMCPHTCVTNLPKPREIHQGKIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJK4</accession>
    <name>T1GJK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003653-RA">
        <property type="protein sequence ID" value="MESCA003653-PA"/>
        <property type="gene ID" value="MESCA003653"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3360242_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003653-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="36" mass="3893" checksum="C196675C2253AB84" modified="2013-10-16" version="1">MNLVKLASAGDIPLKIKSTISFATFAMLPVFSTLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKG8</accession>
    <name>T1GKG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polysaccharide biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKG8"/>
    <dbReference type="STRING" id="36166.T1GKG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003991-RA">
        <property type="protein sequence ID" value="MESCA003991-PA"/>
        <property type="gene ID" value="MESCA003991"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103791_3_0_1"/>
    <dbReference type="OMA" id="IQFYAFE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3560.10">
        <property type="entry name" value="yst0336 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023139">
        <property type="entry name" value="PBDC1-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008476">
        <property type="entry name" value="PBDC1_metazoa/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021148">
        <property type="entry name" value="Polysacc_synth_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410:SF9">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04669">
        <property type="entry name" value="PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis" evidence="1">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003991-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13416" checksum="6DDCC3CA4D4F9040" modified="2013-10-16" version="1">MLISTLDPKTIQLTPFDDVIYNTFRQDFPNLNVAVLSDEDLKDPNEKVRWRNFIEKFNKVDDFSFGTLLRADASKENSPENTLLVVRIQFLAIEIARNREGLNDSIRKNFKPVPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKK7</accession>
    <name>T1GKK7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02071910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKK7"/>
    <dbReference type="STRING" id="36166.T1GKK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004033-RA">
        <property type="protein sequence ID" value="MESCA004033-PA"/>
        <property type="gene ID" value="MESCA004033"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076985_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000169">
        <property type="entry name" value="DNA-binding protein SATB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039673">
        <property type="entry name" value="SATB1/SATB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15116:SF16">
        <property type="entry name" value="DEFECTIVE PROVENTRICULUS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15116">
        <property type="entry name" value="DNA-BINDING PROTEIN SATB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="120"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="122"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004033-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="25290" checksum="8338FCB116DEA773" modified="2013-10-16" version="1">MDEHNNNNNSSNNNNNVDEKSNETSPKNSSKSEEDLDMDEDNNSQIMDDGYRSPSPDPRRDLPFPMVPNSMLQHSFMYMSHYMPGAIAAAQAPHHPHNPGMTPVGVPAGLNLTNMSSEERRKRNRTFIDPVTEVPKLEQWFAMNTHPSHNLILKYTEDLNRMPYRQKFPRLESKNVQFWFKNRRAKCKRLKMSLYDGPQCSQLGGLNPYAHHKYEERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKT8</accession>
    <name>T1GKT8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKT8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004118-RA">
        <property type="protein sequence ID" value="MESCA004118-PA"/>
        <property type="gene ID" value="MESCA004118"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243243_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004118-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12062" checksum="CAC6205DE4855A0D" modified="2013-10-16" version="1">NYHQAQNTAFTPPPLQVQTHHLDIIKNTVSRSYGEEILVSEQIRNLALNNDKTVFIKEPEKRWRPAKKWTTEGISCSTQNSESISSINLFTNQIDPPESKRVSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL77</accession>
    <name>T1GL77_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134759">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL77"/>
    <dbReference type="STRING" id="36166.T1GL77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004271-RA">
        <property type="protein sequence ID" value="MESCA004271-PA"/>
        <property type="gene ID" value="MESCA004271"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984335_0_0_1"/>
    <dbReference type="OMA" id="QCSAYLP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004271-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="13055" checksum="AC3ECCB5EE2CB4F5" modified="2013-10-16" version="1">SASSPRRVAVPVLVKDGKPCSSSSNSASQQQVGGTSNGSSGSGGSVSSVNTLITGDAPHTHSPDTSSALLSYNGAVTGHNSQMLQQPCNNSLMSNSLAMAYRNQNNFMGNSHQQQCGGYLPLQGRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLC5</accession>
    <name>T1GLC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rap guanine nucleotide exchange factor 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLC5"/>
    <dbReference type="STRING" id="36166.T1GLC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004325-RA">
        <property type="protein sequence ID" value="MESCA004325-PA"/>
        <property type="gene ID" value="MESCA004325"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002782_2_2_1"/>
    <dbReference type="OMA" id="VESEMFI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF249">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="5">
        <location>
            <begin position="155"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="8">
        <location>
            <begin position="274"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="414"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="7">
        <location>
            <begin position="597"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="669"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="551"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="885"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="952"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="551"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="967"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="987"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004325-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1217" mass="136538" checksum="109002F16613B351" modified="2013-10-16" version="1">MKYNKKRRAINHTKTMNENLDNKSKMYSNATNPQSDLFFSEKKPKNRSSLAVSDDTMSFSSFSSFSSISTTNRADGERELEGEGGDGEEVDLSGLVESAVDDEDTDDEDLAESMESLTTTTRTPRTIRDCLEKDPTDRTDADLTTLLELTHTLKAFQNTTHSIRRALCSVMVFAVVDLAGTTVLSDGEELDSWSVLINGVVEIEEDENGEKKTLEVGDSFGILPTMDKFYHKGTMRTKTNDCQFNPESRRENIRRHEDTDGRIVLVTEARGRKNRQYVIRGVVEELIEQLVVEGDQDGEDSCGGDGDGGCYIDDFLLTHRTFVESRRVFRQLIDWFKYSETTTIPRTYPSPQEIRDRVTRVILLWVNNHFADFEVDGELLSGLETFDDLLYDQKMLSQRRLLYIASTAKAKPRTLILARNSREENLNFTLVSGGGSGTPSGIFVSDVVKGSRAEEIGLKRGDQILAVNGGKFERNITMTVTRAYELLKQSTHVKIEVRSCLLVLKRILSGEKIDEVDKNGGPQRFFFEEGNDLEGPKKRLQKALAKISILHQQTKPTPLPHSRSNPDLTATPVQTELPPIPALPDHVIRVYRPDHQRISENSTDYSLYELVSDDCVVKQRKLPDQLSDLAERATLVSRYFLKKNSSTEQLSVPADDLTSSALAEILQLDAAELAVQLAIRDFGYFRIVEGVEFVGEVLSTEPGNIKNSSGNLERFSKLVNEEMFWVVSEIVRGESAMKRAKIIKQFIKIARFSKEKRNFNCFFAVLSGLGHGAVGRLRASWEKVPGKYLRVFREMEELMDPSRNMGRYRQLISAELLTHHPIVPFYPIVKKDLTFIHLGNDSRVDGLVNFEKLRMIAKEIRILSRMASADTGGCRGDIRRGTFGGDIGTMKRRKKSTASSSSTAAPANYKKMFEEAQMVKRVKFYLENVKIVEDEDVLHNLSLECEPPNITNNSSNNHFGGSGSISGGSTSAPSTTSSTRKTKNSHRSSPTLSTTSSTSSTSEGRKMFPHAGVPAYPKFGTASPQAVKKMLSLSETSKTRPHTQQPPMPQPPNILRHYSVPTGNSAMLSTWNPSQQLSNFQPQTQQQQNTQHSQMNSAGGNFFSPTISSAPNCYQLPTLSSPQQTGLPTDIIYQQTTTTTPPPPPPFRQIPREYYQMQGSTGNYYPSLILLDLQSFQMKLFFIPSQKRNYQQLPHNLLLYDKFQGNFIKCNTISDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLK3</accession>
    <name>T1GLK3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02027747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLK3"/>
    <dbReference type="STRING" id="36166.T1GLK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004410-RA">
        <property type="protein sequence ID" value="MESCA004410-PA"/>
        <property type="gene ID" value="MESCA004410"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016581_2_2_1"/>
    <dbReference type="OMA" id="QVRMGRN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052220">
        <property type="entry name" value="METTL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496">
        <property type="entry name" value="CGI-41 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496:SF9">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="111"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004410-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="394" mass="44991" checksum="B4E80D343F538FA6" modified="2013-10-16" version="1">MSSLHKQIDAITEFLRPFSAFINCHMVNYLTDNHWEHFISSEVREEIDNFEEAIECFKKLQNFIKSCRNLYLENSKNITSSIEDVILNISEGNLLKKSNDDLSIKEFMSAKKCHEVEVASKFIHEILSLAPVDCVIDAGDGKGYLSSRLALQYKLKVLGIDASTSNTENALDRKKKLEKAWNGLTERANLLSQGITPPRRGKKAKATEIQETRPENYKTCNEFITKDVNFQKLAGEAFNGEFQSFCMTGLHTCGNLASTCLKVFQNQDDFKVLCNVGCCYHLINEEFSQDEFFGNKVLRNLNKDFGFPLSGYLKGQKIQLGRNARMLAAQSVHRTLDKKELPDKSLFYRALLEVLIVGMNENLKDTVQVGKIKNESGTFWNMSRNVTKRRVWDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLT2</accession>
    <name>T1GLT2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="6">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02026470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02026471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLT2"/>
    <dbReference type="STRING" id="36166.T1GLT2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004496-RA">
        <property type="protein sequence ID" value="MESCA004496-PA"/>
        <property type="gene ID" value="MESCA004496"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_6_1_1"/>
    <dbReference type="OMA" id="WMTIRRS"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042541">
        <property type="term" value="P:hemoglobin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015118">
        <property type="entry name" value="5aminolev_synth_preseq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09029">
        <property type="entry name" value="Preseq_ALAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="5-aminolevulinate synthase presequence" evidence="8">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="150"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU910713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004496-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="415" mass="45260" checksum="4FF235A7CF94FC97" modified="2013-10-16" version="1">MPCPFLSRLSANYVRNYAEPLLQTYGSQCPVIARSIFSTPAADTPQVTGATNTTPQAPAAPKVAIAATATTQVRKLASMQQQQPKLVDEIDSEAKHSASGAFPYEEFFHEQILRKKQDHSYRVFKKVNRLAEAGKFPHALDYTSNQEKPITVWCANDYLGMSCHPEVKSAVRHALEKYGAGAGGTRNISGNSLNHENLEKRLANLHQKEAALLFTSCFVANDSTLFTLARLLPGCHIFSDAGNHASMIMGIRNSGVPKHIFRHNDVDHLRSLLKKVDKNVPKIVAFETVHSMTGAICPLEELCDLTHEYGGISFVDEVHAVGLYGDHGAGVGERDNQLHKMDIISGTMGKAFGNIGGYIAGSAKLVDMIRSYAAGFIFTTSLPPTVLCGADKAIEILSSDEGRELRQRHQAMSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLY3</accession>
    <name>T1GLY3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004549-RA">
        <property type="protein sequence ID" value="MESCA004549-PA"/>
        <property type="gene ID" value="MESCA004549"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294835_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577016" description="Kazal-like domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004549-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12095" checksum="B6EF68184357A27A" modified="2013-10-16" version="1">MVISTKMVRLTIALAICFFCVLGAMAHREIDHHTLRPGELLECDDKYQPVCVFDKEFNCYLTVQNMCHVVRLWSEYGPLSLRGETACKEDLLACDPSRYVLKPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM40</accession>
    <name>T1GM40_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM40"/>
    <dbReference type="STRING" id="36166.T1GM40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004608-RA">
        <property type="protein sequence ID" value="MESCA004608-PA"/>
        <property type="gene ID" value="MESCA004608"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1497936_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000027">
        <property type="entry name" value="Eto/mtg8/nervy, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013289">
        <property type="entry name" value="CBFA2T1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379">
        <property type="entry name" value="MTG8 ETO EIGHT TWENTY ONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379:SF14">
        <property type="entry name" value="NERVY, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01875">
        <property type="entry name" value="ETOFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="79"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004608-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="180" mass="20164" checksum="846401157950BA81" modified="2013-10-16" version="1">MIPSSKKTMTSTVLVSVKRQAVIEIQRAVATAENRAADVMAQERIRMEKFFADMTRHSNDRDLDNKSPPISQSAGQNACWNCGRKASETCSGCNLAKYCGAFCQHKDWEHHHQVCGTSRSDVSKHPLPSVPVRGQYQDVRRLLKHNRPNQVDRKQRLPRHLRSPTGATQAAEVAANDAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM94</accession>
    <name>T1GM94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02390004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM94"/>
    <dbReference type="STRING" id="36166.T1GM94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004663-RA">
        <property type="protein sequence ID" value="MESCA004663-PA"/>
        <property type="gene ID" value="MESCA004663"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_8_9_1"/>
    <dbReference type="OMA" id="CIKANTK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="8">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004663-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9630" checksum="C0FF36D58CCCB86B" modified="2013-10-16" version="1">MLDERREEMTSDGPVGMDPEGVIESTWDEVYDNFDDMNLKQELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSVYRKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN99</accession>
    <name>T1GN99_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005043-RA">
        <property type="protein sequence ID" value="MESCA005043-PA"/>
        <property type="gene ID" value="MESCA005043"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640960_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005043-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="9167" checksum="4F435B5621184E17" modified="2013-10-16" version="1">MHCNYDKNYDNGTELFIILSSILCERLKPQILEKTHEFQVDTDNLFINFRQAYDTPQMSEMFKAMNLFGIPFKLNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPI5</accession>
    <name>T1GPI5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPI5"/>
    <dbReference type="STRING" id="36166.T1GPI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005516-RA">
        <property type="protein sequence ID" value="MESCA005516-PA"/>
        <property type="gene ID" value="MESCA005516"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815375_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005516-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="8102" checksum="4C107D4997F7FABB" modified="2013-10-16" version="1">MATEAIKTKAQQEEIIAEFQNMRNEQRMLATKLNTLEMDLKEHNCDRIIENSRRKQEMFPFDWWSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPJ8</accession>
    <name>T1GPJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor IIIC subunit 5 HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPJ8"/>
    <dbReference type="STRING" id="36166.T1GPJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005531-RA">
        <property type="protein sequence ID" value="MESCA005531-PA"/>
        <property type="gene ID" value="MESCA005531"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026463_0_0_1"/>
    <dbReference type="OMA" id="PPEYFVR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001002">
        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001003">
        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.160">
        <property type="entry name" value="TFIIIC, subcomplex tauA, subunit Sfc1, barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019136">
        <property type="entry name" value="TF_IIIC_su-5_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040454">
        <property type="entry name" value="TF_IIIC_Tfc1/Sfc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041499">
        <property type="entry name" value="Tfc1/Sfc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042536">
        <property type="entry name" value="TFIIIC_tauA_Sfc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230:SF5">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIIC, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09734">
        <property type="entry name" value="Tau95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17682">
        <property type="entry name" value="Tau95_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Transcription factor IIIC subunit Tfc1/Sfc1 triple barrel" evidence="6">
        <location>
            <begin position="28"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC subunit 5 HTH" evidence="5">
        <location>
            <begin position="163"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005531-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="56075" checksum="2CEF4B1F8B22113A" modified="2013-10-16" version="1">MSTENLAGTSSDTITVLDKTNLSKELILVEYPGIVKNVDRMIATLGGNRKISEVLSSDNKRLELKFHPENPYNKPANGDPQKANGFLFKVRVKKRQIKTENLGSKAVAHVEIIGHVSKSLRFSIPSNSQKPTHKRNELYFKGSCTPAPKECGNFFNIPNDFYFCPPYSFSRTDSVFNRFFRDNQNAGKTDILRSTNPKSYDSKSIIAFSMSDPVPVTSNAETVKNMAVKYVSDDQLDTVKKMFEECPIWTRIALLYESKISHEKMKAILPHIAYYYVTGPWRTMYVRYGYDPRKEFSSRFHQTFDYRVRFNDGITEYVKVRRQQILHGNPNQGLVLPLDYPYLEMNFLPRSRQCILRYSDIRLPQVQEMLQKVPTPLTGAVCTEKYGWLPQGFDHQVRQIVASYIKVTMTDFFKKQKAEGVEVDDEPMIEDDEAPYTDEEDEEELAEDIKTLTYIVNHCSIEICYHDINSEIRGYIFEAQQENPHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPM4</accession>
    <name>T1GPM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPM4"/>
    <dbReference type="STRING" id="36166.T1GPM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005558-RA">
        <property type="protein sequence ID" value="MESCA005558-PA"/>
        <property type="gene ID" value="MESCA005558"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745145_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005558-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="16540" checksum="BDD20247045B09A7" modified="2013-10-16" version="1">KIQKLKILLGNSSVKRGTPSESYHTDSNPSTGGGGSNLSDVGTNVDSNNQQQTIHSQQQAEQCVPSQQDQQQQCDEVQIVSHHQHVTQIHQPTTQMTHITFVDDESSCDSRWADGDISDVLIKTLADAHANTNTKLDLIHEMFRKPPVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPN9</accession>
    <name>T1GPN9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPN9"/>
    <dbReference type="STRING" id="36166.T1GPN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005575-RA">
        <property type="protein sequence ID" value="MESCA005575-PA"/>
        <property type="gene ID" value="MESCA005575"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_14_5_1"/>
    <dbReference type="OMA" id="CLNIVEX"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF451">
        <property type="entry name" value="KELCH-LIKE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="42"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005575-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="385" mass="43359" checksum="65327558788F6A20" modified="2013-10-16" version="1">MGADLLLGDRPSSPARLSHTSDKHPKITLGELNVLRRHRELCDVVLNVGGRKIFAHRVILSACSPYFRAMFTGELEESRQTEVTIRDIDENAMELLIDFCYTSHIVVEESNVQTLLPAACLLQLVEIQDICFGQLVDIICSDELNVRSEEQVFNAVIAWLKYNASDRRQHLHQVLQHVRLPLLSPKFLVGTVGSDLLIRSDEACRDLVDEAKNYLLLPQERPLMQGPRTRPRKPTRRGEVLFAVGGWCSGDAIASVERFDPQTNDWKMVAPMSKRRCGVGVAVLNDLLYAVGGHDGQSYLNSIERYDPQTNQWSCDVAPTTSCRTSVGVAVLDGFLYAVGGQDGVQCLNHVERYDPKENKWTKVIFFEKNAKRKKEYIPFSLESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPR6</accession>
    <name>T1GPR6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPR6"/>
    <dbReference type="STRING" id="36166.T1GPR6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005607-RA">
        <property type="protein sequence ID" value="MESCA005607-PA"/>
        <property type="gene ID" value="MESCA005607"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156304_0_0_1"/>
    <dbReference type="OMA" id="INQYNIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF118">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="45"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005607-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15847" checksum="8CD350536F86F005" modified="2013-10-16" version="1">MKRLYQINQYNIMASDRIPLNRTLKDMRNSLCKAKTYPKSLPKASVIIVFHNEAWSVLLRTVWSVLNTSPRKILNEIILIDDASEREFLKEPLDDYLAKNLKDIVKIFHLPKRSVRARPPKSAKRNYYLFSKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQQ9</accession>
    <name>T1GQQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial transcription rescue factor 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQQ9"/>
    <dbReference type="STRING" id="36166.T1GQQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005972-RA">
        <property type="protein sequence ID" value="MESCA005972-PA"/>
        <property type="gene ID" value="MESCA005972"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076117_0_0_1"/>
    <dbReference type="OMA" id="LATHHDR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903108">
        <property type="term" value="P:regulation of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057896">
        <property type="entry name" value="MTRES1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633:SF3">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25818">
        <property type="entry name" value="MTRES1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial transcription rescue factor 1 C-terminal" evidence="2">
        <location>
            <begin position="89"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005972-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="24984" checksum="F7845B3753FBE391" modified="2013-10-16" version="1">MLKLFSNYRILNKTLYNCRCISYVNWLSSGTDNLPKLHQSRKEELKSVNFQQVRFKYNKKSSKQDEESDDENDFSDGEFKDDRDSKVIKSRVNSLRADLLLKAGLGMARNKVELVFYENRIRVNGTKLVKKSVQLSVGDEIDVIRGFSTQNPSLLTVSRVEILAAVEKEENIAVQLRRYKSLLIENYKGNNAFKSSQTHTNKIEDLYIDYFLFCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS6</accession>
    <name>T1GQS6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">TRIP-1 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS6"/>
    <dbReference type="STRING" id="36166.T1GQS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005991-RA">
        <property type="protein sequence ID" value="MESCA005991-PA"/>
        <property type="gene ID" value="MESCA005991"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1020447_0_0_1"/>
    <dbReference type="OMA" id="HERAITC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005991-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="273" mass="30611" checksum="11BE022981545740" modified="2013-10-16" version="1">MLQGHERSITQIKYNREGDLLFSCAKDKKPNVWWSLNGERLGTYNDHQGAVWSLDVDWCTSKLITGSGDMTTKIWDVEYGKVLASIPTKSSVRTTNFSYSGNQAAYTTDKAMGQNCELFIIDVRTADSSLSTQDPVLRVPILQHKITSMLWGALDETIITGHENGHISLYDVRNGKQINSVNDHTNSINDMQLSKDGTIELHVYGSVVPVNARDPTKFQHQGFGMLLMEEAERIARDEHGSTKLAVISGVGTRNYYRKMGYQLEGPYMTKLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQU4</accession>
    <name>T1GQU4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQU4"/>
    <dbReference type="STRING" id="36166.T1GQU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006009-RA">
        <property type="protein sequence ID" value="MESCA006009-PA"/>
        <property type="gene ID" value="MESCA006009"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1082939_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="12">
        <location>
            <begin position="47"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006009-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29072" checksum="5FF220CA90FA9090" modified="2013-10-16" version="1">MTITAERERVIDFSKPFMSLGISIMIKKPIKQTPGVFSFMNPLSQEIWAQAALMYDAVFVLVEALNKILRKKPDQFKLAIGPNRKNNQTPANGIGGMNGTSSPSQGNAGNSNGGNGGNNFRSLDCNTSKGWVNPWEHGDRISRYLRKVEIDGLTGDIKFNEDGRRQNYTLHIVEMTVNSAMVKVAEWTDETRLTPLSAKYVRLRPNMDYEKNRTFIVTTLLEEPYIMIRNGPYGEHLKVTIDLKGIAKTWQNFWLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR57</accession>
    <name>T1GR57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin fusion degradation protein 1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02004896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR57"/>
    <dbReference type="STRING" id="36166.T1GR57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006131-RA">
        <property type="protein sequence ID" value="MESCA006131-PA"/>
        <property type="gene ID" value="MESCA006131"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037790_2_1_1"/>
    <dbReference type="OMA" id="YKEHSEQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.50:FF:000001">
        <property type="entry name" value="Ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000002">
        <property type="entry name" value="ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="5">
        <location>
            <begin position="18"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="6">
        <location>
            <begin position="118"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006131-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="307" mass="34971" checksum="EE70BB2E47A50601" modified="2013-10-16" version="1">MFHFENFNMMMFPDGRSFKQKYKAYSVSMLSGNDRSDVEKGGKIIMPPSALDTLTRLNVEYPMLFKLTNGKKNRTTHAGVLEFVADEGKVYLPYWMMRNMLLEEGDFIQIESVSLPVAVYSKFQPQNTDFLDITNPTAVLENALRNFACLTTGDLVAIKYNKKIYELCVLETRPGNAVSIIECDMNVEFEAPVGYQEPTRMKSQEQPAEEPVEYVQEEVPFAGEGNRLDGKKKKDSQLTPQNVVKKESPEEFQTMIIRMDSLNLTETSRPSAIVLLLMKQMSRTMWTASKELGAPLRKPGSNFSLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRE9</accession>
    <name>T1GRE9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Histone-lysine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRE9"/>
    <dbReference type="STRING" id="36166.T1GRE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006230-RA">
        <property type="protein sequence ID" value="MESCA006230-PA"/>
        <property type="gene ID" value="MESCA006230"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_292274_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140938">
        <property type="term" value="F:histone H3 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15565">
        <property type="entry name" value="PHD2_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15566">
        <property type="entry name" value="PHD3_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15567">
        <property type="entry name" value="PHD4_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20144">
        <property type="entry name" value="PWWP_NSD_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05838">
        <property type="entry name" value="PWWP_NSD_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055198">
        <property type="entry name" value="NSD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055197">
        <property type="entry name" value="PHDvar_NSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22908">
        <property type="entry name" value="PHD_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23004">
        <property type="entry name" value="PHDvar_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PWWP" evidence="15">
        <location>
            <begin position="288"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="AWS" evidence="17">
        <location>
            <begin position="424"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="14">
        <location>
            <begin position="476"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="16">
        <location>
            <begin position="600"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="15">
        <location>
            <begin position="875"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="826"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="826"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="845"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006230-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1043" mass="120508" checksum="447941C301B7278F" modified="2013-10-16" version="1">MAKLKKEFKKLEFMRNRNPSIFPNVNKQRKNYTPDLTNNIFCKFCKSTTSSETSHIIKCEKSCGNCFHASCLKGDVKQKRKKSERQSKSKTNVENSNNLTSICDECQADENHLNNCFVCKLPVKFIDDNVADEKSKCLTLNCGKYYHKSCLKMWPQSTWHTGSKQINFICPSHTCHTCVSDNPRGRSVQNSKTKFLKCVLCPASYHRSSACIPAGTQLINNTSIVCPRHDQNDKNHVNVNWCFFCAEGGELVCCETCPASVHVGCLSEPVPSEKYYCDDCESGRLPLYGELVWVRFGTHRWWPSLILPPTETPLNIRRMKHYPSEFVVRFFGTNDHAWISRSRVYLCQDDDWNVQQKNFSSKEGRFIKGIQEAKRIMEIIKTRKLKLEMTDSSSDDVHIPLPYVKVKINKVVSPVKWSDLYDEDKVINCNCSKDIEHPCGPDTGCLNRSLLIECDPKTCPAGDRCENQLFEKRLHPKMKIKYLSGKGFGLIAMENIAAGTFICEYAGEIIDNKEYQRRLQQQAYEKNDDYYFLTLQHNYYIDAGPKGNIGRFMNHSCDPNCESQKWSVRGITRIGLFAIRDISEHEELTFNYMFQSDSHNKKICFCKTSKCSGFIGVKYSEKPVELFPKKLKKCHQKPHFTENISISPFPQICCYCVTELFTKKWSHDLKLLQTDFNPKFMEIKSPSENEKKSRYGRVQKELNIQIKSPKTKDHTLIGFFRLSPKSKDNQVKTKIETLADNLVKKNRNIMNNSQINNENTEEDTVKDEFPKFVVEDTNASHDLNAIQEIVSSDGLAKEDELNDSLESPDFELHEPSGKEIFKSTEDIPSFDHESPNKENLNNNLENEKLKAERSPDSKDSANGSSIDTEIISVKSGELYWAQIGSYPYWPCMISPDPDKKTVSYKDNLLLKVTYHVRFFADNGRRSWVKEDRLLPYHGREPFVQRFSKITPKKKRNSIDFQHKLYRKTVNNKTWDLAIEEADSLVPYSLQERIKLFEAILDKSRNEVKAFVDYQKAVYTILRIVCYQPKFKLINGLDRCLQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS24</accession>
    <name>T1GS24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02395074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS24"/>
    <dbReference type="STRING" id="36166.T1GS24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006472-RA">
        <property type="protein sequence ID" value="MESCA006472-PA"/>
        <property type="gene ID" value="MESCA006472"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_57_6_1"/>
    <dbReference type="OMA" id="EACLNVR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001077">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF26">
        <property type="entry name" value="FI22810P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006472-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="387" mass="43332" checksum="D824BEE07CAB1FB3" modified="2013-10-16" version="1">MGPSGAGKSTLLDALSGFKTTGVDGSILLNGRRRDLPTFRRMSCYITQDDRLNPLLTVEENMHIAAKLKLGPNVPYEEKASRIEDILILLGLYSHSDTLAKRLSGGQKKRLSIALELLNNPTVMFLDEPTTGLDSHSCNKVIELLKKLATQGRTIICTIHQPTAKLFEEFDQVYVLSAGNCMYQGATTKLVPFLQSVDLSCPMYHNPADYIIELACGEYGFDKCDTLKNATSNGSSWTWFNDTAKLYKAEKLMKLHPIQKKCNKKSLNDTSFTNQMSCLMERGIIKIKRDTTLTHLRVIVNVLVAIMLGSLYVGAGNEGSRVLDNYNLLFAILIHHSMTTMMLTVLTFPTEMNLLLKEHFNRWYSLKAYFTSLTILDIPISVGIFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSF6</accession>
    <name>T1GSF6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006611-RA">
        <property type="protein sequence ID" value="MESCA006611-PA"/>
        <property type="gene ID" value="MESCA006611"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534308_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006611-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20502" checksum="6D66C3261E313ECC" modified="2013-10-16" version="1">MAINSSSEIYEAFNVVRFFVLSSGLFLCSRSSSGTISNSKLSIIILFFFYILYLISFGITFKDTLEFMETFFNSEMAMKTILFYPLANIFYVTAIFLTTFVNRGKMVRLIALIVNLDKCGLRIHYKKVVVFSSYFVVFITLYFVLYFLYDLFVTKWPYCRNWPIEFRSTTLGVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSH7</accession>
    <name>T1GSH7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSH7"/>
    <dbReference type="STRING" id="36166.T1GSH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006637-RA">
        <property type="protein sequence ID" value="MESCA006637-PA"/>
        <property type="gene ID" value="MESCA006637"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123886_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000004">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF374">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="4">
        <location>
            <begin position="7"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006637-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="13118" checksum="C485C2A83C2F38F1" modified="2013-10-16" version="1">MLLDEFCKLQAEKGPCRAIFHRYFYNVNSNKCELFTYGGCDGNENNFRSQKECISACIVKFSCAILGVEISFRPNNGNNLLLITRTTMDVLETPLEMGKWKLVLERRKFPPLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTE7</accession>
    <name>T1GTE7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006977-RA">
        <property type="protein sequence ID" value="MESCA006977-PA"/>
        <property type="gene ID" value="MESCA006977"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136311_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006977-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12947" checksum="B9B1C3640FBD1940" modified="2013-10-16" version="1">MLEFMEDRNQEKKVLRERKRKGFSTTASCCKNKNGDLIKNKGEVLMQCKYFFKELLNGNESRMPLKEIINVIRISVSEFQPVAPQNQSWTIPLGSTAAQVSGRFAPSKVKEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ7</accession>
    <name>T1GTQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ7"/>
    <dbReference type="STRING" id="36166.T1GTQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007098-RA">
        <property type="protein sequence ID" value="MESCA007098-PA"/>
        <property type="gene ID" value="MESCA007098"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220904_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007098-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="25687" checksum="105CBC569FD28FA0" modified="2013-10-16" version="1">MGNHQNQIDLTELGVSLDNLKFEYDKCAIESNITSSLPELHISRADDSHSYQSIDKMNIILSPTGSDMTQSTQESNQNTISETVGFRDDQPTTPALKEVRFYREQDTMSRSKSYTDNSCARRRRRRKSRARCSSENQINHSNLQNSQSQNNNIQKPNRQHNNDSDGESVCSTCSSSSSSTDDYVYKIPTRKHYGGVRVAYVPNDAIAYEKKRKQISGDAENKNCVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTV2</accession>
    <name>T1GTV2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007146-RA">
        <property type="protein sequence ID" value="MESCA007146-PA"/>
        <property type="gene ID" value="MESCA007146"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984113_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007146-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14702" checksum="74345938E461814E" modified="2013-10-16" version="1">MESRTISMRNINAEDAAAFERFHIHTQLTDDGSLNIEDLDHRVKEMYEGARLFQGTRISATFLQFRPPATYPGRFIRIQTIEYAFVQYCTERSLQTYSIYERNNFAAPKICGLYNIWGNNFCISVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTZ7</accession>
    <name>T1GTZ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02108950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTZ7"/>
    <dbReference type="STRING" id="36166.T1GTZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007196-RA">
        <property type="protein sequence ID" value="MESCA007196-PA"/>
        <property type="gene ID" value="MESCA007196"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_761386_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007196-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="364" mass="40918" checksum="E740D3985F0AAFDF" modified="2013-10-16" version="1">MMTIAANMWNSVAERINSFEKQKQQTQNQQQDNGYTFIDPSKTHRVPNTTLKAFQKNAVQSYFERQQQASNSNNILRPHSLQSKGTPKEVPQSYMIRSDFQQQQQHRPTTKPQINDDSFAFPESNGSNITCNSNESSSSSNLHSPPPPPPRSRSLMPLRRSSSASDYADFRDNYIREKQMYLINDCGVPPPPPPRGRPSMPITLVRRPSFQRQNDAMIMGPVIMLDNWVPERPPKNPNLRIPSPDLPPPPPTLTLPLHCSDDPLPPPPPEILKAPSPTQLSPSRRNSFAGSPNHKSFYRTSSIIENLPPPPVIPKKPTTFEIAQSRPASSLATQSNRAHKVVLNGKLEVHRLTLRSGNVHIMTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU67</accession>
    <name>T1GU67_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02037527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU67"/>
    <dbReference type="STRING" id="36166.T1GU67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007275-RA">
        <property type="protein sequence ID" value="MESCA007275-PA"/>
        <property type="gene ID" value="MESCA007275"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_3_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009066">
        <property type="term" value="P:aspartate family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000001">
        <property type="entry name" value="L-asparaginase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="4">
        <location>
            <begin position="57"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007275-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="302" mass="32926" checksum="82B9BF0B7C213326" modified="2013-10-16" version="1">MRGNRTSKISANSLDAFDASNVPPLARIGINIDVDYRLIFRPCTISRFSISSELDDNVGLLRLFPSITSATCRAFLQPPMKGVVIQSFGSGNIPSNRQDILQQFKEAAERGVIIINCTQCPNGSVVGIYETGKILQDLGVISGFDMTPEAALTKLSYVLGKSEWTLEQKKQMMQTNLRGELTSAKQADIEEFDLVDAVARSLHISTPQELSQLGNTLFPAMLNAAVAAGDIAKINNLKSYGADLAGVNVDNRTALHIACREGNVEVVNMLLLNGVPVHTRDKYDKTPLIEAISTDRHETLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUB8</accession>
    <name>T1GUB8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUB8"/>
    <dbReference type="STRING" id="36166.T1GUB8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007328-RA">
        <property type="protein sequence ID" value="MESCA007328-PA"/>
        <property type="gene ID" value="MESCA007328"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307603_0_0_1"/>
    <dbReference type="OMA" id="HESTHLN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000039">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="4"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="47"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007328-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="211" mass="23198" checksum="9D7D42883CB44753" modified="2013-10-16" version="1">EKPFKCEHEGCDRRFANSSDRKKHSHVHTSEKPYFCRINGCDKSYTHPSSLRKHLKIHGNQDEKPSPNVFDSDGEESSSSSIVTGGAQTPPSATNTLSCRGIKTSPPHIKSESCASLPANSNPKSPSSSILLPTSPFMHPPLIPSNSPHSNISQPTSSTNTSSMLAVGNHQHHNHFLYHPSQHSSEWYHTPSSTVSANDSLNHFSHHPHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV79</accession>
    <name>T1GV79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV79"/>
    <dbReference type="STRING" id="36166.T1GV79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007665-RA">
        <property type="protein sequence ID" value="MESCA007665-PA"/>
        <property type="gene ID" value="MESCA007665"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010739_1_0_1"/>
    <dbReference type="OMA" id="INAEVSC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003109">
        <property type="entry name" value="GoLoco_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF32">
        <property type="entry name" value="RAP GTPASE ACTIVATING PROTEIN 1, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02188">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00390">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50877">
        <property type="entry name" value="GOLOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="3">
        <location>
            <begin position="268"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="511"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="518"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007665-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="638" mass="72321" checksum="5793F9EC023474AB" modified="2013-10-16" version="1">MDTTKAFSNSEKDLIDSAGAQQDFFELLERAQSSRLDDQRCVLPAYFEQDKENTNPSTPSKTTVHKTVTARITAAPSTKNFNRRNVNRKSVRSKPSRKSKALLAKNPKTTKLVNENNEVLTKSKKVQQMLEACLNKNAPFPMIYSHPASEYWIDEANYSDHQSLPKKTWKAKLEFDETARIYRKYFMGKEHLNFVAKDDKLGPILMSIKTEEQTIRVLLRVSTGSLHKEIPVSSTAKSPLALAQMMTDNQITTDNFVPVFCPNASQQIVKFDEHSVVLNFKFGVLLQKFGQTTEEELFCNNSQSPAFDEFLECLGERIVLQDHRGFRGGLDITNSQTGDPQQLQRKRHIGNDIVAIIFQEENTPFCPEMIASNFLHSFIVVQPIDPNTQNTKYKVSIAARNGVPFFGPPLPYPSIFKKGPEFKEFILTKLINAENACYKSEKFARLQLRTRTALLTTLTEELTSETNKFLGITPMISPTASPKSPEAPPHQQQQTTGKIFNTVRRALTFRSKRDEDSSSMSSTTSNNSLNSSDVSHETISEDKCQHGSETDTGVDSMPEEDDNISAACKCQMELKFTETCLREELNRLKCDKLSLLKQNVTCQRDIKRLKKKELCLHTELEVAGKEILRLRSLLKEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVH1</accession>
    <name>T1GVH1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007775-RA">
        <property type="protein sequence ID" value="MESCA007775-PA"/>
        <property type="gene ID" value="MESCA007775"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973029_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007775-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14996" checksum="C944AB6563971BC1" modified="2013-10-16" version="1">MRMEPISEMLGSIERYNSDHLPILQDRCFGKVTGIFDSIRNYVSHVVGITYQTIDKDLLKRSWEELMTKLSTPGLRRTTGRFQEKLFLLLHKTLRSKSRILQRRLNSTTCKPLWLNVFKINRERGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVS5</accession>
    <name>T1GVS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02141040">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02141041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVS5"/>
    <dbReference type="STRING" id="36166.T1GVS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007891-RA">
        <property type="protein sequence ID" value="MESCA007891-PA"/>
        <property type="gene ID" value="MESCA007891"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1040413_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF7">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="63"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007891-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="29843" checksum="5483EF12D3FE9195" modified="2013-10-16" version="1">SNPLQTSDGSPAVVPEHPTATTNQWTEINQSPQKEYQQIVVPPNALGYLRHDNNMLYITENCVVIGRNSSTSSVHFHVAQNSFVSRKHFQVLFDTNTGDFYLSCLSKNGVFVDDVFQRKNSEPTKLPKMCTFRFPSTDIRIIFESFADSDGTIQAKQSLEPTSIDEKAIYSSPLQISIPKSEQKSPYPSPTGTISAANSCPTSPRHNFQDYSVPGNLFQPPSTSNYSNNEHEKPPYSYAQLIVQSIAAAPDKQLTLAVLAYYSMYLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVW9</accession>
    <name>T1GVW9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVW9"/>
    <dbReference type="STRING" id="36166.T1GVW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007937-RA">
        <property type="protein sequence ID" value="MESCA007937-PA"/>
        <property type="gene ID" value="MESCA007937"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_6_0_1"/>
    <dbReference type="OMA" id="RNRTIMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="225"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007937-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="299" mass="35026" checksum="4F860C04428FF464" modified="2013-10-16" version="1">MCIGETGLGKSTLMDTLFNTSFESAPSPHTLPSVKLKAHTYELQESSVRLKLTICDTVGYGDQINKDDSFKAVVDYIDAQFEAYLQEELKIKRSLSSCHDSRIHVCLYFICPTGHGLKSLDLHEFVRVGSKSMRARQYPWGTVQVENESHCDFVKLREMLIRTNMEDMREKTHTKHYELYRQKRLEQMGFTDVDSENKPVSFQQTFEAKRTNHLAELQSKEEEVRQMFVQRVKEKEAELKDSEKDLHAKFEKLKKDHSEEKRKLEDARKKLEEEFVEFNRRKAQMATHHTLTLGKNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVX5</accession>
    <name>T1GVX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fumarate lyase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02149797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVX5"/>
    <dbReference type="STRING" id="36166.T1GVX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007943-RA">
        <property type="protein sequence ID" value="MESCA007943-PA"/>
        <property type="gene ID" value="MESCA007943"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000015">
        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="3">
        <location>
            <begin position="182"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007943-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26187" checksum="CAD853026FC951DA" modified="2013-10-16" version="1">FLDLKIRSNILPLGSGAIAGNPLNVDREFLAKKLGFDGVTSNSMHAVGDRDFVVDFIYCASMASTHLSRLAEDLILYATKEFNFVKISDKFSTGSSLMPQKRNPDSLELVRGISGIIFGNLSGIMMTVKGTPSTYNKDLQYDKKFLFDSFDQLKKVLDITAGVLSTLSVNGETMETALSSDMLATDWAYYLVRKGVPFRTAHHTIGQVVALGEKKKTTEIGTRGYIDTYWAMNLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW74</accession>
    <name>T1GW74_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02086952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02086953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW74"/>
    <dbReference type="STRING" id="36166.T1GW74"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008046-RA">
        <property type="protein sequence ID" value="MESCA008046-PA"/>
        <property type="gene ID" value="MESCA008046"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054272_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18019">
        <property type="entry name" value="DEXHc_Brr2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="158"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008046-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="419" mass="47486" checksum="13AB89F18DC35CAE" modified="2013-10-16" version="1">MTEVCSNTLEKLGLPLHEFPKARLLIYQHVVLLWERNVESRWADGISSIGLDFLVKQETSDHTQGVAGQVDGVRNILDFGELTFTQGSHFMANKRCQLPDGSFRKQKKGYEEVHVPALKPTAFEDKEELQPVDKLPKYVQSVFSGFKTLNRIQTRIWKAALESDENLLLCAPTGAGKTNVALLTMMREIGKHINEDGSINADEFKIIYIAPMKSLVQEMVGNFGRRLSSYGLTVSELTGDHQLTREQIAGTQIIVCTPEKWDIITRKGGEKTFTSFVRLIIIDEIHLLHDERGPVLEALVARTIRNIETTQEDVRLIGLSATLPNYEDVANFLRVKPDKGLFYFDNSFRPVALEQQYIGVTEKKALKRFQVMNEIVYEKTMEHAGKNQVLVFVHSEKKLEKQLEPLGICAWKKTLLAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWJ8</accession>
    <name>T1GWJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02143136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWJ8"/>
    <dbReference type="STRING" id="36166.T1GWJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008175-RA">
        <property type="protein sequence ID" value="MESCA008175-PA"/>
        <property type="gene ID" value="MESCA008175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707096_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="1">
        <location>
            <begin position="160"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="391" mass="44762" checksum="F179B244DAC23733" modified="2013-10-16" version="1">MMQRLITQQAVDQKGARYITDALWMLMEHNIEEVKVLQTVTLLLTTNTVVHGDTLAKSLVLCFRLHYTKNITIVNTAGATIRQLVSLVFERVNLERELNKNHVEIATENSETPPPPKEELQTFAQDAYMLFQDLVQLVNADQPFWLIGMTEMTRTFGLELLEAVLTTFSPIFFENEKFTLLLKDRSCEEAIARLNQATGNPQTPVFYTVNQILYPLYQGCETKDLKIIKFCLGMMQRLITQQAVDQKGARYITDALWMLMEHNIEEVKVLQTVTLLLTTNTVVHGDTLAKSLVLCFRLHYTKNITIVNTAGATIRQLVSLVFERVNLERELNKNHVEIATENSETPPPPKEELQTFAQDAYMLFQDLVQLVNADQPFWLIGMTEMTRTFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX50</accession>
    <name>T1GX50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02149821">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX50"/>
    <dbReference type="STRING" id="36166.T1GX50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008390-RA">
        <property type="protein sequence ID" value="MESCA008390-PA"/>
        <property type="gene ID" value="MESCA008390"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_981034_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045891">
        <property type="entry name" value="ZIP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39 ZINC TRANSPORTER , MEMBER 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133:SF0">
        <property type="entry name" value="ZINC_IRON REGULATED TRANSPORTER-RELATED PROTEIN 102B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577222" evidence="8">
        <location>
            <begin position="18"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008390-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="284" mass="30693" checksum="37C672DFAF56FCCF" modified="2013-10-16" version="1">MIVFLAIMLHKAPAAFGLVSFLLHEKIERQQIRKHLGIFSLSAPLCGLITYLGIGQEHKDTLNSINATGIAMLFSAGTFLYVATVHVLPELTASHGHGHGAADTEHLLEENRLPSNNNKTDGVALGAAATTDHQDVEMIVFLAIMLHKAPAAFGLVSFLLHEKIERQQIRKHLGIFSLSAPLCGLITYLGIGQEHKETLNSINATGIAMLFSAGTFLYVATVHVLPELTASHGHGHGAADTEHLLEENRLPSNNNKSGVLKNSELIFIVFGALLPVILTFGHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX98</accession>
    <name>T1GX98_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX98"/>
    <dbReference type="STRING" id="36166.T1GX98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008440-RA">
        <property type="protein sequence ID" value="MESCA008440-PA"/>
        <property type="gene ID" value="MESCA008440"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362093_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008440-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10781" checksum="FA0563A38690D5B8" modified="2013-10-16" version="1">MNFVVAMDNLQTISPRTLLVFMFWLDILNSDSKNYMALLLMGASYQENDKEKAAVYLRRAVKESPENPTVALQGLSSCAEVEELPNIFGKLLNLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY82</accession>
    <name>T1GY82_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small monomeric GTPase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02091054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY82"/>
    <dbReference type="STRING" id="36166.T1GY82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008804-RA">
        <property type="protein sequence ID" value="MESCA008804-PA"/>
        <property type="gene ID" value="MESCA008804"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148706_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001686">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008804-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12969" checksum="2A3CAEAC32B64A08" modified="2013-10-16" version="1">MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIGEFIVFFPDFFFTIFILFFVIFLFPPSFFLRFCDGEKGDSKLEVFLGWEERGAINRQNVHKSFPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYC7</accession>
    <name>T1GYC7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02129096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GYC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008852-RA">
        <property type="protein sequence ID" value="MESCA008852-PA"/>
        <property type="gene ID" value="MESCA008852"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042128_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000156">
        <property type="entry name" value="Voltage-gated sodium channel Nav2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="79"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008852-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="267" mass="30736" checksum="D699883A9FE28943" modified="2013-10-16" version="1">MGCTNTSTAKYWTRVRSAVLSVVDTPAFEWFVLVLIFASSITLCFEDIYLDISGLFWILXXXXXXXXXXXXXXXXXXXTILDFIIVFVSVFSLLIEENENLKVLRSLRTLRALRPLRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCLIRDKWDCFDQNYTWINSKITFDHVGMGYLALLQVATFEGWMEVMADAVDARGVDLQPAREANLYAYIYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ80</accession>
    <name>T1GZ80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009178-RA">
        <property type="protein sequence ID" value="MESCA009178-PA"/>
        <property type="gene ID" value="MESCA009178"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815347_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7566" checksum="2B4853083473A864" modified="2013-10-16" version="1">MEARWVDLVARDAKELGIPNWRLSFLYEIANKYLTLFEGLSSTPPSIKNLKTEIRCLLVAVNTTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZB4</accession>
    <name>T1GZB4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02079901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZB4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009212-RA">
        <property type="protein sequence ID" value="MESCA009212-PA"/>
        <property type="gene ID" value="MESCA009212"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729572_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009212-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8090" checksum="011BDB5FD9DF8BC5" modified="2013-10-16" version="1">MSVLRNLSRSLIVRRIPNVTKQTSICLIEPKDTVSPTPCILQNRSFVTKTLENDGIFKKVLKKVGFAENSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZU5</accession>
    <name>T1GZU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homeobox protein SIX1 N-terminal SD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146955">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZU5"/>
    <dbReference type="STRING" id="36166.T1GZU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009402-RA">
        <property type="protein sequence ID" value="MESCA009402-PA"/>
        <property type="gene ID" value="MESCA009402"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2216481_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031701">
        <property type="entry name" value="SIX1_SD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390:SF61">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16878">
        <property type="entry name" value="SIX1_SD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox protein SIX1 N-terminal SD" evidence="4">
        <location>
            <begin position="2"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009402-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12342" checksum="89B25A8B2F51576C" modified="2013-10-16" version="1">MFTKDQIECVCEVLFQSGDTDRLSRFLWSLPLTNDIQSSESVLKAKAALAFYEQNFFEFYYFQNVGGGSGGEVTIILENEKLTDATFYRIIRGYDHRVLALNMEDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZU9</accession>
    <name>T1GZU9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009406-RA">
        <property type="protein sequence ID" value="MESCA009406-PA"/>
        <property type="gene ID" value="MESCA009406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461102_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="9758" checksum="D7DF776BE4B1B92B" modified="2013-10-16" version="1">VKLHNDLNTLTTERPGSVSQRLGGIICTSTQSLQTITTTASKDFDDLSSNAHPTKVAKCICPSLALVDPCQMMGHRRGENKSIEKRTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0L6</accession>
    <name>T1H0L6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02155525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0L6"/>
    <dbReference type="STRING" id="36166.T1H0L6"/>
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        <property type="protein sequence ID" value="MESCA009697-PA"/>
        <property type="gene ID" value="MESCA009697"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111391_0_0_1"/>
    <dbReference type="OMA" id="GQNFNEE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009697-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="20762" checksum="6FBF17A1657EF71B" modified="2013-10-16" version="1">VIYIIFQLSKILKLFVGWDKQSYGYHGDDGNSFCSSGNGQPYGPTFTTGDVIGCGVNLVNNTCFYTKNGIDLGIAFKDLPSKLYPTVGLQTPGEEVDANFGQEPFQFDQIEDMIKQMRAETKAAIYNFPMPINQGDPTALLHKMVSSYLVHNGYSSTVEAFAKTTGQNFNEEISSIKNRQSKSKYCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0T0</accession>
    <name>T1H0T0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0T0"/>
    <dbReference type="STRING" id="36166.T1H0T0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009769-RA">
        <property type="protein sequence ID" value="MESCA009769-PA"/>
        <property type="gene ID" value="MESCA009769"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2043917_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009769-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="14127" checksum="F3BB21009755950E" modified="2013-10-16" version="1">MFCASNERFQNITEKTKLLAQKCTQQISSHHKPTSQKSKTTDDIEKKNNSTNSFKYLCACTGGTLRSFRRKKHDNGGVGTVRWYVKQPTWRLWGEEREKDAIFTVYLKKVRYHRPTPITAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0W6</accession>
    <name>T1H0W6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0W6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009812-RA">
        <property type="protein sequence ID" value="MESCA009812-PA"/>
        <property type="gene ID" value="MESCA009812"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504276_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577792" evidence="2">
        <location>
            <begin position="20"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009812-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9552" checksum="B0DFEA0CE10846C9" modified="2013-10-16" version="1">MGKVLIILTICLLVGVAMASPPRRGLRRREQTPVKDSGDEVSTEASETMDKVESVEHSKADLLPKRKFKFTKNRKAAEVVKDPSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H105</accession>
    <name>T1H105_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H105"/>
    <dbReference type="STRING" id="36166.T1H105"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009855-RA">
        <property type="protein sequence ID" value="MESCA009855-PA"/>
        <property type="gene ID" value="MESCA009855"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3093465_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009855-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="5973" checksum="6E02B6B729A52330" modified="2013-10-16" version="1">MEKLKLSDMSTEDIVKNAGKIIYQVHDELKDKDFRLELSIVGKETDGVHQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1D4</accession>
    <name>T1H1D4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02154928">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1D4"/>
    <dbReference type="STRING" id="36166.T1H1D4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009993-RA">
        <property type="protein sequence ID" value="MESCA009993-PA"/>
        <property type="gene ID" value="MESCA009993"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_1"/>
    <dbReference type="OMA" id="TRSHGHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009993-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17320" checksum="8899A1404F703189" modified="2013-10-16" version="1">ALARREQRLNELKDSLPAELRENVYPIKCDVSDEADVIRVFRWVDEHLGGVSVLVNNAGIVRQTLLTKEDNTSDIKAVIDTNVLAVVYCVREAYQQMMKNKVDGHVVIVNSIAGHSVPHIPGFNFNIYPASKHAVTAMVETYRQEFSNAGTNIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H210</accession>
    <name>T1H210_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H210"/>
    <dbReference type="STRING" id="36166.T1H210"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010239-RA">
        <property type="protein sequence ID" value="MESCA010239-PA"/>
        <property type="gene ID" value="MESCA010239"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929967_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010239-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14311" checksum="45E6B97D1F35B8F4" modified="2013-10-16" version="1">MSNLTLRTIILITISFNAHPSGLPNEFWPTNFPLHPHFNPALLPQGIFPHYKLPNFQTLLSQYMGLSNLNGFLGYPQNLSVHTPSQSSQISPHSSISPRESTPPTSAAATNNNNNNNNISTTKTETPGSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2M0</accession>
    <name>T1H2M0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor L-glutamate and glycine-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02381732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2M0"/>
    <dbReference type="STRING" id="36166.T1H2M0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010470-RA">
        <property type="protein sequence ID" value="MESCA010470-PA"/>
        <property type="gene ID" value="MESCA010470"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860228_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000178">
        <property type="entry name" value="Glutamate receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="11">
        <location>
            <begin position="38"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010470-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15151" checksum="F7F3AC5A0ED5967E" modified="2013-10-16" version="1">MSISKKNKFGLCAFTPIPTIIDKSGEITPPCGVNLLTALLTVTEPPEGTTYEGNERYEGYSMDLIDAIAKQLGFKYVFKMARDGKYGGWDAEKKSWNGLIKELLDGEADLGICDLTMTSARRKAVDFTPPFMTLGISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X6</accession>
    <name>T1H2X6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="T1H2X6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010582-RA">
        <property type="protein sequence ID" value="MESCA010582-PA"/>
        <property type="gene ID" value="MESCA010582"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3392740_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010582-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="32" mass="3869" checksum="F817C5E89EC0964A" modified="2013-10-16" version="1">MILLRAYCVKSCHIKSTYPPKKRIWTKKFLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H331</accession>
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        <recommendedName>
            <fullName evidence="1">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
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            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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                <person name="Hughes D."/>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="ELMO" evidence="1">
        <location>
            <begin position="58"/>
            <end position="159"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18390" checksum="3E0F518ACCDB698F" modified="2013-10-16" version="1">MLKGFEIVKEKEIGHEMAHQLHVLQTLKLGLMESRLRTEWNPQDTESQNKILDLKQRAFREAINNAANNAANSLYPIPNIKEEQYYKKLGFRNTTCPGMDFKVVPPGLLALDCMIYFAINFNIDFTKVIHASAYRTDEHECPFARSSIELIRVLCDVFR</sequence>
</entry>
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    <accession>T1H3E4</accession>
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            <fullName evidence="1">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
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            <authorList>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AAA_12"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010769-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17987" checksum="0F763BFF6958C049" modified="2013-10-16" version="1">KKDHGVFFFDVSDGQTSVGQNNSCFNTYEAIALVEITEQLLENGFKAENIAIITPYREQAHEISRSFNIFDHPYIGTINEVQGLEFDIVLFSAVRTDQFGFLDSPKLNVALSRAKYLLVIFGNEQFLTNDLDWRNLIAYTVQENAYQSYVSSNFKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3J3</accession>
    <name>T1H3J3_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="1" key="2">
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            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3J3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010821-RA">
        <property type="protein sequence ID" value="MESCA010821-PA"/>
        <property type="gene ID" value="MESCA010821"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3399816_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010821-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="31" mass="3688" checksum="DBFC889276DE01D7" modified="2013-10-16" version="1">MYLVFNFNLHFYFAPSYPVQLDLLPNKILGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4M8</accession>
    <name>T1H4M8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
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            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA011241"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:positive regulation of signal transduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Sex lethal, isoform D"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
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        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011241-PA"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="111" mass="12506" checksum="280C5080A4BE9C65" modified="2013-10-16" version="1">MANSTNSLNSGTNNSGTNLIVNYLPQDMQDRELYSLFRTIGPINTCRIMRDYKTGYSYGYGFVDFGSEADALRAINNLNGITVRNKRIKNIQTPGSYYSLFPHVLEPSRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4R6</accession>
    <name>T1H4R6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4R6"/>
    <dbReference type="STRING" id="36166.T1H4R6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011283-RA">
        <property type="protein sequence ID" value="MESCA011283-PA"/>
        <property type="gene ID" value="MESCA011283"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1236295_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011283-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="26023" checksum="BAC0D1D59836EC47" modified="2013-10-16" version="1">MACLIVLWSIKSFPQTSILFPLMLVVMIGIRKLLDLVFTRRELKILDDIMPEITKRQADDDLNRLEDGEDHNNEHMFGKQQLNEAFHSSNMNITIPLSNENSDRNTALNLTEEVNRTSIWKNVNLNDQRESRRIICDQIIIPVPLKPRKINGNPNSLSCNEDVTTLDKPLNLSSAQYHKQLLSMPEEKEIEEIIKRVIKRNIGADIVLRSPNYPHQKHQFEFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5A9</accession>
    <name>T1H5A9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">26S proteasome regulatory subunit Rpn6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5A9"/>
    <dbReference type="STRING" id="36166.T1H5A9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011486-RA">
        <property type="protein sequence ID" value="MESCA011486-PA"/>
        <property type="gene ID" value="MESCA011486"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029573_0_0_1"/>
    <dbReference type="OMA" id="KIMVNHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="26S proteasome regulatory subunit Rpn6 N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011486-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="24440" checksum="D8631598A0CB4958" modified="2013-10-16" version="1">MAGATLFERAQQISSTNREEGISLLNKIVREQEVAANDDELIRIKEQGILQLGELYKQEGKAKELADLIKVTRPFLSLISKAKAAKLVRSLVDLFLDLEAGTGIEVQLCKECIEWAKQEKRTFLRQSLEARLIALYFDTGLYSEALTLGGQLLKELKKLDDKNLLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKMQSALDLQSGILHAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5D4</accession>
    <name>T1H5D4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5D4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011511-RA">
        <property type="protein sequence ID" value="MESCA011511-PA"/>
        <property type="gene ID" value="MESCA011511"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1143684_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011511-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27534" checksum="F51930EDAFBA7E2B" modified="2013-10-16" version="1">MCLRIAAPFQLFCMSDSPPKFENSSLLVSVREDHEPSAPPYYYQPAPTAPPLQHSISVQPSTSSNPTLNRNISERIVALQKQVVVPKPVVVQNQAVIQKQFVGSKPKIDIESKPLPPIPVASNNNNRQVYEDDENIYEELEPRPEYDDEYFSDSDFDSTDYEDDIDSASESTKNATKEEFKRQYESNSFQDDPLRNLQKVLLTGKQASSSQNNVIKREVPQMSPVQQNISKRGEPERTSDIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H671</accession>
    <name>T1H671_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EnsemblMetazoa" id="MESCA012204-RA">
        <property type="protein sequence ID" value="MESCA012204-PA"/>
        <property type="gene ID" value="MESCA012204"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661611_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012204-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8710" checksum="CC6EE233BD907FCC" modified="2013-10-16" version="1">NKTPEIMDTKSDIDSEVEQQNYSLQNNMEPKNMEELTVFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6R6</accession>
    <name>T1H6R6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6R6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012399-RA">
        <property type="protein sequence ID" value="MESCA012399-PA"/>
        <property type="gene ID" value="MESCA012399"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1063839_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012399-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="262" mass="29420" checksum="E9BF623AE80D4F04" modified="2013-10-16" version="1">LKGAWQYLDNICNLSQDVKDQYLRNTKKTDGYNEPDEECLSEVSSQAFNITTLKINSFHEDEDPVPGFDKHINELAKDFKMLSNFLLQSLAVSLGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6U2</accession>
    <name>T1H6U2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6U2"/>
    <dbReference type="STRING" id="36166.T1H6U2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012424-RA">
        <property type="protein sequence ID" value="MESCA012424-PA"/>
        <property type="gene ID" value="MESCA012424"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565008_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="8931" checksum="4060C1516A27C7FB" modified="2013-10-16" version="1">QMSPPSQFHLSNLLLLTLAEKSVNDKILLDFLTTNSKFHTNMASNVLSQSGLYSSALLLGLTRGDAIDCLSAMVKVIGHHFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H720</accession>
    <name>T1H720_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H720"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012502-RA">
        <property type="protein sequence ID" value="MESCA012502-PA"/>
        <property type="gene ID" value="MESCA012502"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_1_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000019">
        <property type="entry name" value="transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000007">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="13">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012502-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="308" mass="33835" checksum="41176E4172E1DE11" modified="2013-10-16" version="1">DSNKVVCFAHPESPLVEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLSGGDLSTVIGPGTGSASFDSFGTPKYQNRRTMSSSDRALISAFKEISMMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKEIGRCFKLTLKALETSVDLITTADFMSRFCANLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASEHKKSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPEDFKFVTPIDQLPQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7F4</accession>
    <name>T1H7F4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil-helix-coiled-coil-helix domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CHCHD7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7F4"/>
    <dbReference type="STRING" id="36166.T1H7F4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012691-RA">
        <property type="protein sequence ID" value="MESCA012691-PA"/>
        <property type="gene ID" value="MESCA012691"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175044_0_0_1"/>
    <dbReference type="OMA" id="QELSYKC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051040">
        <property type="entry name" value="COX23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012691-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="8293" checksum="3B9CE951F373F119" modified="2013-10-16" version="1">EQELSYKCLDKNNYDREKCEVYFANKNCRSFWDKVKTDRRKKGILPVMPDLEDRQKIKEEYFKTRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7L1</accession>
    <name>T1H7L1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FKRP stem domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7L1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012748-RA">
        <property type="protein sequence ID" value="MESCA012748-PA"/>
        <property type="gene ID" value="MESCA012748"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_738856_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055105">
        <property type="entry name" value="FKRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052613">
        <property type="entry name" value="LicD_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13627">
        <property type="entry name" value="FUKUTIN RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13627:SF31">
        <property type="entry name" value="RIBITOL 5-PHOSPHATE TRANSFERASE FKRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22921">
        <property type="entry name" value="FKRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FKRP stem" evidence="1">
        <location>
            <begin position="3"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012748-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="375" mass="43726" checksum="0FFC89C4A80153AE" modified="2013-10-16" version="1">TNKHIKKSITVVFRSFYNFENDLKSSINNILEVIPNIPIIILQDGVSYPPINYKVIVFKQHAVKFFSLGFDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALQDGRKMFTSYHTKQRKLELRRKQFKEMYKKLQIKKLIKHSYRKHNLKTDTSYSHNIILQFSSSNMSLPLTTEIDFIGCERNSKSCIGPVYNLKPFYYYLQKHTPPCCIEKLKNVFFHVLDEFENVGIRYWLDNYALKSAIETNRLSPDAFDIDISFNSFDLERSNSLKKSQMKPFADNLGYYWIKATDGHYFRVQFSKMNQIGVNLLPFEISGNEVRPNGFFGWKAHEFSSEFLHPMSSVIILGKNVMCPNNVREFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA37</accession>
    <name>T1GA37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000082-RA">
        <property type="protein sequence ID" value="MESCA000082-PA"/>
        <property type="gene ID" value="MESCA000082"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1688746_0_0_1"/>
    <dbReference type="OMA" id="NQEWLGT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="18015" checksum="C84578536270B972" modified="2013-10-16" version="1">MNPTITDIDALRMENMKVSYNISNYATEKLNGSSNFMTWSFTIKTQLELDDLWCCIERDENVIDVAKNRKYKIILSVKIRGEVTAKNVCTKLNNMFKDTGLKRRITLLQKTTSTSFQLGEIGFAISDEWLASLMSKGLPLEYRPMIMTLSCIYRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA38</accession>
    <name>T1GA38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetylglucosamine kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA38"/>
    <dbReference type="STRING" id="36166.T1GA38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000083-RA">
        <property type="protein sequence ID" value="MESCA000083-PA"/>
        <property type="gene ID" value="MESCA000083"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045127">
        <property type="term" value="F:N-acetylglucosamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039758">
        <property type="entry name" value="NAGK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862">
        <property type="entry name" value="BADF TYPE ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862:SF0">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="46457" checksum="956B05F6F571B5B1" modified="2013-10-16" version="1">MKYFGGIEGNSKKSHLLICDKDGRMVAETFGLGVDYRVLGITEAAKNVADLVDRAKEEAGIEQCTKLYTLGICLSRIDRSRLWEVRDVSIPEKVARCKRMEALGLHFDSCKYYKEPDDKASNDELIEELESWFYDVADHYIISRDLNGGIASISDNGGMALKVGIGSTSFLLNPDGTCFSCGGWGSFLGEEGNLLRFLEGFNLGKYSTDLVWKIIQDHFQIEEKIDLLQHFYKNFDREFFAGLCPKLALAAQKGDKLCEFLFSEAGSNIAKHVLALLPNVHRELISNQSLNIVCMGSVWQSWDLFKYSFENELSSKEIRYDLKFFQLLNGYAIGALYLGVNSVGIFKHRHFKANAHEFFRHFKDRGESFGVVEHNAAFLPIDLKSQSRINVFSASDSKLDDTASEDRIFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAA6</accession>
    <name>T1GAA6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02089695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAA6"/>
    <dbReference type="STRING" id="36166.T1GAA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000156-RA">
        <property type="protein sequence ID" value="MESCA000156-PA"/>
        <property type="gene ID" value="MESCA000156"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443359_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF70">
        <property type="entry name" value="NK7.1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="41"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000156-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10370" checksum="DE104ED27EE98D87" modified="2013-10-16" version="1">MECQIGISKPKKYELDNDNSSHTSFKEDSTDDSDSASGDMQRKKKARTTFTGRQIFELEKQFEIKKYLSSVERTDMAKLLNVTETQVKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAT4</accession>
    <name>T1GAT4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02058469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAT4"/>
    <dbReference type="STRING" id="36166.T1GAT4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000345-RA">
        <property type="protein sequence ID" value="MESCA000345-PA"/>
        <property type="gene ID" value="MESCA000345"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1671324_0_0_1"/>
    <dbReference type="OMA" id="THERPYP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000345-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="18076" checksum="2B29CCB9BC97FB4E" modified="2013-10-16" version="1">MHMFTHTGEKPFKCNYCERKFGDHTSRKRHERLHRNERPFACQVCGKTFTYASALKAHKVVHTGEKNFHCIPCGKSFPQKHNLTSHLNTITHRNIVKTLEADSTTTIIEEDDEDKEIVNDGNVEYTIEAGDIMNVNVDGSTIILSDNRIPMGYQFHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAW9</accession>
    <name>T1GAW9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02118156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02118157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02118158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02118159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAW9"/>
    <dbReference type="STRING" id="36166.T1GAW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000385-RA">
        <property type="protein sequence ID" value="MESCA000385-PA"/>
        <property type="gene ID" value="MESCA000385"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1410305_0_0_1"/>
    <dbReference type="OMA" id="INYEYRA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000385-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="22251" checksum="8A693D1A70B4E7A4" modified="2013-10-16" version="1">MEEELNFYIQNAKIKDIKNYMKSKGIQKLGIPHYISNGIFEKDGKSYKFIILPRFGKSIGHFINYEYRAFLPQTIYRLTLQILDALEYIHKRNYIHGDLKADNILLGYGSKSKSHINIVDFGTSLPVSNNFLESPDSTSFGTWTYNARDTGKVKRSPAFDSDKENINPRSKKRKHETNLLNESKLPTKILKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBD7</accession>
    <name>T1GBD7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBD7"/>
    <dbReference type="STRING" id="36166.T1GBD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000569-RA">
        <property type="protein sequence ID" value="MESCA000569-PA"/>
        <property type="gene ID" value="MESCA000569"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145026_2_0_1"/>
    <dbReference type="OMA" id="DIACKFL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000189">
        <property type="entry name" value="Neogenin isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF100">
        <property type="entry name" value="FASCICLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000569-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12692" checksum="E1AB045CFF3438EA" modified="2013-10-16" version="1">LRDDFRAQPQDTRVAAGETALLVCSPPKGIPDPTVIWIKDGELVKTNDKKSHRVLLENGALFFYRTVHSKKEQDGGVYRCVAKNQVGIAESRNATLQIACTYPSLFYIYLINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC43</accession>
    <name>T1GC43_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GC43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000841-RA">
        <property type="protein sequence ID" value="MESCA000841-PA"/>
        <property type="gene ID" value="MESCA000841"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_872344_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000841-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="36094" checksum="5E335008B7D968C0" modified="2025-10-08" version="2">MLNERNEDDEHSTKGKQSELLRQLKKEEGVKEASHHNTICSPDELRNLLKIQESDRSITRKRPPNNGDDSSCKKGEEKNRKYSLEGNPMLAKLLSEKPKNPPILPPTQVNIKVIPDIKPVNNRSNDHSXXXXXXXXXPDIKPVNNRSNDHSLCNKFNMQPDVSETQKNQMLPNQQQQLTMQSAMNSRGFQINMDENQPFSNCPITSTILSASGGTNQHQQHKNNTENDPELAKIINEFIDFQEIDDARTQIQSGYSEKDEKINEIEKSLMMCETTNFNTNCNLDSNAAFSLMSPQNPSKTHPPLLDFQPSMPLSSDIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD30</accession>
    <name>T1GD30_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001209-RA">
        <property type="protein sequence ID" value="MESCA001209-PA"/>
        <property type="gene ID" value="MESCA001209"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017866_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588199" evidence="1">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001209-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="13904" checksum="57D92CA87B7CDF55" modified="2013-10-16" version="1">MFSRINTFFIIAVGYFVIASGEFHKCKIGDVKCLKEVGTELLVEAKGFSDINLISLDPMNFGDMRIQKDEKSPVNIDLKMKNAQAFGLAGTKIIKASGFGKDLKGPHSLTFHNDATSLVGDYDINGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD60</accession>
    <name>T1GD60_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ADP-ribosylhydrolase ARH3</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ADP-ribose glycohydrolase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ADP-ribosylhydrolase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">O-acetyl-ADP-ribose deacetylase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Poly(ADP-ribose) glycohydrolase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">[Protein ADP-ribosylarginine] hydrolase-like protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">[Protein ADP-ribosylserine] hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>alpha-NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:77017"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02184750">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD60"/>
    <dbReference type="STRING" id="36166.T1GD60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001241-RA">
        <property type="protein sequence ID" value="MESCA001241-PA"/>
        <property type="gene ID" value="MESCA001241"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237703_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF24">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001241-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="24962" checksum="D73277A2721AFE51" modified="2013-10-16" version="1">EYFTNPNRGYGRGTIAVLTKLRQTKASDPLKPAQESFGGIGSQGNGAAMRIAPVALFCYNKSDKLQDMVRQCSLVTHSNDLSVNGAILQALAIHYCVNENPEDFKNEELLEYLCTEFEKIEGTLDEFGLATEKVYGKKLEDVKKLLLKEDPSIDELMNKLGNNATALGSVPTALYCFLKAHKQPIKGIDMKLIKKLQKYFIKDQTSWDNITLTSEVTFNNNVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD88</accession>
    <name>T1GD88_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GD88"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001269-RA">
        <property type="protein sequence ID" value="MESCA001269-PA"/>
        <property type="gene ID" value="MESCA001269"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978529_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF22">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="62"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001269-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="285" mass="31988" checksum="33A45FFA17DD968F" modified="2013-10-16" version="1">QILFTNFSLFIESQYEXXXXXXXXXXXXXXXXXXXXXMLFNFRTNATHAENMDALYDDSIAYGIAMAIASVGMFIFGIISVDAFNLAALKQTTKMRMKFFESTIRQDIGWHDKTTDQNFALKMADNIERIRDGISEKISHFSYLIGVCVCCICIGFGFGWKLTLVLCFYIPVIIAWGGVGLAGRLVEEILGSIRTVVSFGAESTECKRYDKLMEPACKAVKVKGGFSGFGDGILKGFLFINCGAAFWYGTYLVVEDRGTDNPTYTTNVLMMLLNVVMLLRYYNWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDN4</accession>
    <name>T1GDN4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rapamycin-insensitive companion of mTOR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196554">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDN4"/>
    <dbReference type="STRING" id="36166.T1GDN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001423-RA">
        <property type="protein sequence ID" value="MESCA001423-PA"/>
        <property type="gene ID" value="MESCA001423"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850176_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001423-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15738" checksum="634AFBE6DB46859E" modified="2013-10-16" version="1">MDKNKDARELRYTSSRLALLSVLKSWTGILEFCDPSRPSGLKAVVDILYLNQLDVRKAILDLFYELIGLPQIIWTDEYSVALSLVDPSDFQDAWLLNNGFVAMEGRCILPSLANRVPNICEQNLAIILYSFLETGLLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDR1</accession>
    <name>T1GDR1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GDR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001455-RA">
        <property type="protein sequence ID" value="MESCA001455-PA"/>
        <property type="gene ID" value="MESCA001455"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020602_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="510" mass="57274" checksum="9037D983C591E034" modified="2013-10-16" version="1">FSAFTFKGDVLQEYNDYVLQRPSLKPEPPTAAAAKPGTRKSKRAATTTNLVYLESGSDSEDEFDDIPLAKRMAMSSSKLKNKKLDTNIKEEKDDNKKKSPKSKKEPQSGTTAPDSPVPSSXXXXXXXXXXXXXXXXXXXXDSPVPSSSEKPLKSKKEKEKEPAVVFKPSEISSLQGKTFPSLVVLAKPWLKVKDMSSTRSKLDSKVKLVLMLTPNKFTEWLIQEGLLKAEQKCSLHKASDHKLGVYSDAAKFPYSGGYVWISDCCPTRFVSVFHGSLFEAAPHPPSCILKLIYHWACQTNIQNVVQWVKVDNVYVKGVYTWLRAVCTLAIQQKCKLLGGAGKYVEVGVISLGTTSQDGQQRQVKVEVLGVFDYTERTIRLKAIEPVSDADRNYKKRFQTILEPLNRWVHKDSIIAIDLTVDKMTLYQLGFKNIVQAAANDTTAKHTNTAVMDYLRRIVPRMFQNTLSLLSRQMIQQFLDELVATNPFKDWSVIPNSEMSTTTTPKRLKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDV7</accession>
    <name>T1GDV7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIH1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles. Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription. Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1. Positively regulates the assembly and activity of the mTORC1 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDV7"/>
    <dbReference type="STRING" id="36166.T1GDV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001508-RA">
        <property type="protein sequence ID" value="MESCA001508-PA"/>
        <property type="gene ID" value="MESCA001508"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062696_0_0_1"/>
    <dbReference type="OMA" id="CIHEAIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF0">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001508-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="21412" checksum="DD14664F8C6D4001" modified="2013-10-16" version="1">MCIKCYKCNTDDKFFINLCQTDDIPPPEDISEKELTDILNSNEPSTFRIPMSISQPRLTLDKSDNKVDVIDVAISAIFLRKCERSLLFRDFLIACIHEAINNKYEITLNSENFIVLKNRKSIGNLISHRVENRDVKKGYDMLETSDPEEKIKIMNSTGSSKKPLIQEITSSEKQQLWTKIKRSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE47</accession>
    <name>T1GE47_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001605-RA">
        <property type="protein sequence ID" value="MESCA001605-PA"/>
        <property type="gene ID" value="MESCA001605"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779555_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001605-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="16904" checksum="9BBA8173D6806C84" modified="2013-10-16" version="1">MIGLKILAAMDQRLRQALPNNQDLEFGGLIIYLFGLTFPLNVNKIETFEKNNEHIAVIVWYQKWEKENKEKLNRGLGQNSQITTHIFCTTCLTNFTNKTNYDMHKCICVKSEYPNSGKSIKFDAIKKMHPSPIVVYADFECELVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GES0</accession>
    <name>T1GES0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transforming acidic coiled-coil-containing protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACC family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GES0"/>
    <dbReference type="STRING" id="36166.T1GES0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001841-RA">
        <property type="protein sequence ID" value="MESCA001841-PA"/>
        <property type="gene ID" value="MESCA001841"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_354628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1700:FF:000001">
        <property type="entry name" value="Transforming acidic coiled-coil-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039915">
        <property type="entry name" value="TACC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007707">
        <property type="entry name" value="TACC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL CONTAINING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924:SF10">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL PROTEIN, ISOFORM K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05010">
        <property type="entry name" value="TACC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transforming acidic coiled-coil-containing protein C-terminal" evidence="9">
        <location>
            <begin position="642"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="558"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001841-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="792" mass="89490" checksum="85E3ACD349E9EB8E" modified="2013-10-16" version="1">MSVEVIQEIPMENNDIKSFENLSSINEKQIKMDDDQPENKSKSEVETLQTIFNSDCKFNKFQNNSIGPGTSSKIQSPAKSETASSAKKASHHHTPKSKVQENFRNALLIDDDKTLSEELPDIVKVNPLPQQVLSTTTSDSPTTTALKALEKLKLDSTQLSVNPTSSTEHSDEEFTECQSYSALHATSTKNWEQSQNGVGDELPPDSTTKSANTTQEIVDSSNKNLSLTLNKSFPVIKSPPQTTFPVSPTPDAEIKEYTKENGRQSITTLNGTFAASSEALNSTINLNQDNGLEAEDRQNVANCTASFMSANATIPMHKMDSGFASTNLEENHNVTASFESANQTMNIELNTTNPVNSTLPMNSMNVTTSEDQSNQRTFVPEDNDLNVTTSGINEVPEKPSSLDNSEINTTTDVETLQKSLKDVSVTLNNEEKEVNTTTCLEDNVEQIENVANCKGNESYSENDANRNTNEVLSEDLPVVPDSGKEQEVNAKLSETVDMQESMSMDVDMEDLEENNKQPEPTEINKDCNDCNENVLSPMEVEENEIVKPESLRDFDNQIFLSDKEKSNLNNEKEEKEEKTFQLEDFNAEFKIRNIRMKNLKVNQKVYLETMTLKRNLWNQNKGKKLFLSASQKKIKLFPNDWSEKEQLSQNFEKELNEIKADRDSNYYHLTSLETTFSDLNIKYEKNKELLSQVKASEEKLLTERKVEAENLKMQEQRYEKMKSHAMMQLEIANQKIEDFRKQHTLEVTRLKALLKKEEISRAAVSDQLQQKTKENEQLMKICDELINSQQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF48</accession>
    <name>T1GF48_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02065618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GF48"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001977-RA">
        <property type="protein sequence ID" value="MESCA001977-PA"/>
        <property type="gene ID" value="MESCA001977"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_599329_0_0_1"/>
    <dbReference type="OMA" id="SIASMNN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026079">
        <property type="entry name" value="CDR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19232">
        <property type="entry name" value="CENTROCORTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19232:SF7">
        <property type="entry name" value="CENTROCORTIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="306"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="463"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001977-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="498" mass="56034" checksum="50F01C57C5B3BFDA" modified="2013-10-16" version="1">MFQNHKYLSLELSLKQESRKSPPNTLHIEGDDIHEDSPNPYKDLVDKYEALLEVQRQSNPRRRSSVVVAANSLAEEMKAAKANDQIEKQDKEERVCIRDALSEAETSSSGYSDETSTKGTQTDGTPGYFLCSIGNSDVDGEDCKFTNXTDGTPGYFLCSIGNSDVDGEDCKFSIYDDSSQSIDSRFRNRPEYRELFKEIFAVLKKAADNKAEGEKLPLLDDEEQETVTKGVLPVPPVTPAAENMPEPSEIDCDSQSIISSAISEQSFAMSECITKLERKTAKKQHAAVVAAQVLVPLKREPLDYLSTGLGHKKRNRNRKNRSWNPEARSESPSGNPSSPRHGSGKKRKDYRPFDFQSTPSPLATPPNRQQPPSATQEWDGSSLIIYNKRNYNQAAGSTPRTSQSGEVDLTAVQYRPSTLFQDFHKLKKLDMSYAEVLRKASADDSHHHYYHYQCQMQNAAAGTGFGHSQGHKKSHRSNNFYHQQRHHKSHHQQQQQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF52</accession>
    <name>T1GF52_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02035147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF52"/>
    <dbReference type="STRING" id="36166.T1GF52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001981-RA">
        <property type="protein sequence ID" value="MESCA001981-PA"/>
        <property type="gene ID" value="MESCA001981"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154836_0_0_1"/>
    <dbReference type="OMA" id="PCEYFDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF72">
        <property type="entry name" value="HLH54F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="33"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001981-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12636" checksum="45658326308A3722" modified="2013-10-16" version="1">MPRRKRSDSPCEYFDSAFDEDSSSCNSRSETGVPRNAANARERARMRVLSSAFCRLKTKLPNIPLDTKLSKLDTLRLATNYIKELKSLIEGDNVNAEEIPIKFNHSLSSWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFN7</accession>
    <name>T1GFN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFN7"/>
    <dbReference type="STRING" id="36166.T1GFN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002182-RA">
        <property type="protein sequence ID" value="MESCA002182-PA"/>
        <property type="gene ID" value="MESCA002182"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344634_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF12">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 14, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="18"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002182-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="22913" checksum="1819442E4538F10B" modified="2013-10-16" version="1">MNPQSHSFCSMSSRDWCPVTTFRPSLSTCFTTGANQPLNCHRMNADNAECAIWNQLQTQSVKNTEMVIEMDTIGIGISFGCGWTGGLKVSWVKKNVDANGAFELLTVGLHTYSGDNRYTVEFQYPHNWRLKISNARKSDEGTYECQISTHPPRVMQISGHEVLHEVVNEAAMAFYHKQFALAAFLDIEGAFSKLHHCVHQGCTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGB2</accession>
    <name>T1GGB2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGB2"/>
    <dbReference type="STRING" id="36166.T1GGB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002426-RA">
        <property type="protein sequence ID" value="MESCA002426-PA"/>
        <property type="gene ID" value="MESCA002426"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067164_0_0_1"/>
    <dbReference type="OMA" id="PAHEECT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002426-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13699" checksum="1120CA6E863F647F" modified="2013-10-16" version="1">MVKKLVYIHLYLKLLENCRGKVVECSAKDDHNIKEIFKTLLSVSKYSAPASNDNSQTGLKRRSSAYVSASSSRNKNRTSSPAPIQENGEGSKLKPRSRSLIRRASRKNKQALSDTTADDCLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH56</accession>
    <name>T1GH56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH56"/>
    <dbReference type="STRING" id="36166.T1GH56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002741-RA">
        <property type="protein sequence ID" value="MESCA002741-PA"/>
        <property type="gene ID" value="MESCA002741"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1572426_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002741-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19701" checksum="21DE6EC67681B629" modified="2013-10-16" version="1">MELGDFNAYEKMGKNFEYKLRRTGQPTSYNRFVPGYGRLQREDNKNRLTSSKLTQRTKSENDHIDRYGCSDSIWNGLLGIPELYYRDLAARNVLVADNNIVKIADFGLARLIKEDEYEARVGARFPIKWTAPEAANYSKFSIKSDVWSFGILLTELVTHGRIPYPGWYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHJ6</accession>
    <name>T1GHJ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02174655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHJ6"/>
    <dbReference type="STRING" id="36166.T1GHJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002900-RA">
        <property type="protein sequence ID" value="MESCA002900-PA"/>
        <property type="gene ID" value="MESCA002900"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2405692_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002900-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10201" checksum="FA78C1B4AE257B02" modified="2013-10-16" version="1">MDDIRLEGKHLPLPPAMNGTQWGQATVAPNLERGCPSNKPCSNVICPEPFECVDLWNEYQCTCGEGRVMSPDRKGCMDRNECLDLPCANGATC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHS8</accession>
    <name>T1GHS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytosol aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.13.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinylglycine-S-conjugate dipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Leucine aminopeptidase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Prolyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-cysteinylglycine + H2O = L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:28783"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:28784"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-benzyl-L-cysteinylglycine + H2O = S-benzyl-L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:62568"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:145802"/>
            <dbReference type="ChEBI" id="CHEBI:145803"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:62569"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an S-substituted L-cysteinylglycine + H2O = an S-substituted L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:60444"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.13.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:60445"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.23" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02140572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHS8"/>
    <dbReference type="STRING" id="36166.T1GHS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002988-RA">
        <property type="protein sequence ID" value="MESCA002988-PA"/>
        <property type="gene ID" value="MESCA002988"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013734_1_3_1"/>
    <dbReference type="OMA" id="SQMINQM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF25">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M17 leucyl aminopeptidase N-terminal" evidence="16">
        <location>
            <begin position="95"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="15">
        <location>
            <begin position="210"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002988-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="404" mass="44463" checksum="71436583831E3117" modified="2013-10-16" version="1">MSIFNLSRSICSGRPYSKTLIRWQSSSNPAVNQLLTLQQMEICADPPSRAAIFGVYADENDKNDGGILTPSGWNYNIKKTGGRLLEALRLSGPMPRKGEARPIAIVGLGKECLGFNSYEVIDEQKETIRRSTAAACRQLCKLQTDRIEVESLGHAESAAEGAALGVWKYQEFKMLKDKQDIPTIDLYSIKDEPCDFDGWRIGLQKAAAQNLTRTLQEMPSNILTPTAFAQTVVEVLCKTGINVEVKVEGWAEGHGMNPFLAVGRASCEAPIFLELSYYGAPSDERPIVLIGQGITYDSGGLSLKKYKYLRHLRGDMTGAAVVVSTMRAIASLRLPINIRALIPLCENVLGCNAFKPGDCYKSMAGKTVEVESTDKEDTLNLIDALLYAQNFCPKFIILEQRLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI77</accession>
    <name>T1GI77_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003146-RA">
        <property type="protein sequence ID" value="MESCA003146-PA"/>
        <property type="gene ID" value="MESCA003146"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3071066_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588309" evidence="1">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003146-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="53" mass="6203" checksum="9A9FBF1733C68C9E" modified="2013-10-16" version="1">MYGSLICLIFFECTDTSIVTTKYVSNNNNNTIMMSNRHHSDVFRDTIMIHLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIM6</accession>
    <name>T1GIM6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02077301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIM6"/>
    <dbReference type="STRING" id="36166.T1GIM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003309-RA">
        <property type="protein sequence ID" value="MESCA003309-PA"/>
        <property type="gene ID" value="MESCA003309"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_4_1"/>
    <dbReference type="OMA" id="CTERHGG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588334" description="Peptidase S1 domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003309-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="27546" checksum="A43256181AD05F06" modified="2013-10-16" version="1">MIKTLLCLGFLGTALSFPFDGRVVNGTTAKEGEYPYVVSLRSTSGSHSCGASIIAPDWILTAAHCVTGRIPNTTTIQYGVVNISVAGPNIAKVKKIIYHENYNPSSIINDIALLQLETPLKYNYINVAPIQLPPQGYEVPANSQLIGWGLEKTGGKVQTQLQEVNLKCYSDKECQQRHNYRTNKDNICAGVDEGGKGQCSGDSGGPLTFGKYQVGLVSWSVKPCTIAPYPGVYTKVSHYINWINANMKAIFAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIP3</accession>
    <name>T1GIP3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CLEC16A/TT9 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIP3"/>
    <dbReference type="STRING" id="36166.T1GIP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003326-RA">
        <property type="protein sequence ID" value="MESCA003326-PA"/>
        <property type="gene ID" value="MESCA003326"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1039335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901096">
        <property type="term" value="P:regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045820">
        <property type="entry name" value="CLEC16A/TT9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF0">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19439">
        <property type="entry name" value="CLEC16A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CLEC16A/TT9 C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003326-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="30111" checksum="09A37FD1FC71B9CD" modified="2013-10-16" version="1">MDNVLDLNNSDLIACTVCYKDNTKQRRFLVIDSQQLILVEPDSKRLGWGVAKFVGFLQDIEVAGDKDDSRFLHMTVHRGGASHNRAPLLSARFQFDDHIRCMAAKQRLTKGRSKARQKKMHQIAHLLEMPGQTVLDSPAFQAPSKWSASGSSSRSKSDQRPTMFVNNRVPGFAAALRRENVPSAGISRVQIAQNRSVEGIRNEGQSRSRRRKSSNSSTTHPEAIRKLPPETDLLLDLGIIVQECPGRDQKKFPWKTSNILVVTVQRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ14</accession>
    <name>T1GJ14_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ14"/>
    <dbReference type="STRING" id="36166.T1GJ14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003453-RA">
        <property type="protein sequence ID" value="MESCA003453-PA"/>
        <property type="gene ID" value="MESCA003453"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256782_0_0_1"/>
    <dbReference type="OMA" id="SHRWPSI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11779" checksum="ECF241D30C44495F" modified="2013-10-16" version="1">MACPFLRRDESLSKQQSTDQVGSWQLEDELLDTADSDALSLTHLQMAIQLLTEPANEDLNLAVTSMVGKYSHRWPSIENLKIDLKTLKNKPDYDYLADIQATLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ60</accession>
    <name>T1GJ60_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleoplasmin core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoplasmin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02031778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ60"/>
    <dbReference type="STRING" id="36166.T1GJ60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003503-RA">
        <property type="protein sequence ID" value="MESCA003503-PA"/>
        <property type="gene ID" value="MESCA003503"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097031_1_0_1"/>
    <dbReference type="OMA" id="FNDGEIY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004301">
        <property type="entry name" value="Nucleoplasmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024057">
        <property type="entry name" value="Nucleoplasmin_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036824">
        <property type="entry name" value="Nucleoplasmin_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747:SF18">
        <property type="entry name" value="GEO09167P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747">
        <property type="entry name" value="NUCLEOPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03066">
        <property type="entry name" value="Nucleoplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69203">
        <property type="entry name" value="Nucleoplasmin-like core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin core" evidence="5">
        <location>
            <begin position="6"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15917" checksum="DE7F17148C1F6374" modified="2013-10-16" version="1">MEMETFYGVTLSEKDPLAQFDVAESEFENNQKLIIKQIVLGYEAANGEFNVVQAETKSEDKDIKIPIAVLKAGETRTLTPNLEFPHSSVTFKLITGKGPVHIHGQHIISETDMEEVYYEEEEEGDDDEVQTNGKNIKRKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ73</accession>
    <name>T1GJ73_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003517-RA">
        <property type="protein sequence ID" value="MESCA003517-PA"/>
        <property type="gene ID" value="MESCA003517"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944363_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003517-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6969" checksum="70EEF7AE6A20BB31" modified="2013-10-16" version="1">MSGDGEEFTVVKLEANEIQRNGMENVVYLTRHLRDLLYMKGREQNLTAITYSNFGRMSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJI5</accession>
    <name>T1GJI5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose-methanol-choline oxidoreductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003634-RA">
        <property type="protein sequence ID" value="MESCA003634-PA"/>
        <property type="gene ID" value="MESCA003634"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906678_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003634-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6929" checksum="914A005DBB28D0CB" modified="2013-10-16" version="1">MGSSEDCQAVVDSRLKVHGINGMRVMDCLIMPNVIFLKGNWGGCQEETYSERLLLMKRHGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJJ8</accession>
    <name>T1GJJ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJJ8"/>
    <dbReference type="STRING" id="36166.T1GJJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003647-RA">
        <property type="protein sequence ID" value="MESCA003647-PA"/>
        <property type="gene ID" value="MESCA003647"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="OMA" id="YESDVGP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588336" evidence="3">
        <location>
            <begin position="17"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003647-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="9563" checksum="4C1F2F1DB41F9D42" modified="2013-10-16" version="1">MKFAIVFAALFALAVAAPAKPASGSDAQILSDGTAINAQGQLKRVDPENAAIVQSGSFRFVADDGQTYEINYIADENGFQPQGAHLPVAPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP7</accession>
    <name>T1GJP7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MHD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GJP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003697-RA">
        <property type="protein sequence ID" value="MESCA003697-PA"/>
        <property type="gene ID" value="MESCA003697"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074740_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098831">
        <property type="term" value="C:presynaptic active zone cytoplasmic component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019992">
        <property type="term" value="F:diacylglycerol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061789">
        <property type="term" value="P:dense core granule priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099525">
        <property type="term" value="P:presynaptic dense core vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016081">
        <property type="term" value="P:synaptic vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027080">
        <property type="entry name" value="Unc-13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480:SF12">
        <property type="entry name" value="UNC-13, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01145">
        <property type="entry name" value="DUF1041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MHD1" evidence="3">
        <location>
            <begin position="92"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003697-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="323" mass="37220" checksum="E63188E32C83DA91" modified="2013-10-16" version="1">VDPNEAKXXXXXXXXXXXXXXXXKVDPNEAKRESDDHGPKIDNVDFWHKLIALIVSVIEEDKNSYGTVLNQFPQELNIGQLSAVTMWGLFAVDMKYALEEHEQHRLCKSSEYMNLHFRVKWLYTNYVKDVPPLKGSVPEYPAWFEPFVMQWLNENDDVSLEYLISAIARDKKDEFQKSSEHALFSNSVVDVFTQLTQCFGVVSKLECPDPEIWKRYMKRFAKTIVKVLLKYADIVKTEFPEHMSDERVACTLMNNIQQIRVQLEKMFESMGGDNLETDAANILKELQQNLNSSLDDLACLFAVSLEPRITASVKELGXYVLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK17</accession>
    <name>T1GK17_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="6">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 8">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02035598">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK17"/>
    <dbReference type="STRING" id="36166.T1GK17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003828-RA">
        <property type="protein sequence ID" value="MESCA003828-PA"/>
        <property type="gene ID" value="MESCA003828"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_4_2_1"/>
    <dbReference type="OMA" id="QRPHHDI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6 8" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="9">
        <location>
            <begin position="26"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="28"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="31192" checksum="16835F84E21F0782" modified="2013-10-16" version="1">MITREKTDILELFQDAKSSNSFLRVAAPMVRYSKLEFRSLMRRHNVQLCFTPMITADCFANSPSARFSEFSTNSEDTPLIAQFAANKTSDLLNATEIIAPYVDGVDINCGCPQSWAIAKGYGCGLLRNPELMSDMVKTLRRNFSNKLSISVKIRLLHSENFSSTVELSRQMEACGVSFLTLHGRTPYSKTADPVIHQGIKVVKDSLRVPLIANGNIKSIEDALEMHSITNCDGVMAASGLLTNPSLFDEDNDGITSFKCIQDWANIAASAGEILHFQTFHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK29</accession>
    <name>T1GK29_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003843-RA">
        <property type="protein sequence ID" value="MESCA003843-PA"/>
        <property type="gene ID" value="MESCA003843"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3417469_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003843-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="26" mass="3134" checksum="924E698785630301" modified="2013-10-16" version="1">MFLVFHQGEVTLKRTLWCVVRCILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKE9</accession>
    <name>T1GKE9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKE9"/>
    <dbReference type="STRING" id="36166.T1GKE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003972-RA">
        <property type="protein sequence ID" value="MESCA003972-PA"/>
        <property type="gene ID" value="MESCA003972"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028286_6_1_1"/>
    <dbReference type="OMA" id="MFLVYNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="157"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003972-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="314" mass="36303" checksum="0B32CA45FE8C4C8B" modified="2013-10-16" version="1">MDFTKQKIVLGVGMGISLSTFLAFYLYKKFKKGSKIPKEWRKVGKLVEISYFPLRSCSGIELDEGFCGEFGLGEGNFHDRIFQLVDIKSGEIGFAGKRPKLYEIKTQKIDDNILKVSAHGMEDLIIDVHEIAKGKEYYHDVYGFVVPLLDCGEKFDIWFSRFLLGKDTGLKLSMSRTPVVKTNWFRRTPKVNFYMATTTSSLKDLNSRLENPVSNRQFRGNFMIETVDEAYSEDDWRWLKIGNEAIFKWNAPCYRCILPNVRPITAQRSKDGEPFKTLKRYRMRKGEKEPIYGTYFDAVQNGVVRKGDEVFVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKG0</accession>
    <name>T1GKG0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02112263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKG0"/>
    <dbReference type="STRING" id="36166.T1GKG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003983-RA">
        <property type="protein sequence ID" value="MESCA003983-PA"/>
        <property type="gene ID" value="MESCA003983"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066675_0_0_1"/>
    <dbReference type="OMA" id="TGSANCI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF456">
        <property type="entry name" value="CD63 MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003983-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="261" mass="29537" checksum="B098A0582DEA1B8D" modified="2013-10-16" version="1">MSFLTGSANCIKYTLFAFNLVFVITGIILIAVGVGVAGTFNEYDSFLQAKFYSITTFLIVIGVFIFIIAFFGCCGAIKENYWMILIFSVLLGIIFILELAAGISGYVLRNDAETLIGKQLEETMAKYKEDPTIGSLWDYTQREFSCCGTNSYKDWKLGSIDLNNYTVSKVPMTCCDMPQQGGGRRPSFNGVDLCKEQTERQTDSVLYIWILQMYPFFKECQQITQPLIRPQVLPKNDLQANRVFLEESIAKEVEIKHQIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKM3</accession>
    <name>T1GKM3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Synaptotagmin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKM3"/>
    <dbReference type="STRING" id="36166.T1GKM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004050-RA">
        <property type="protein sequence ID" value="MESCA004050-PA"/>
        <property type="gene ID" value="MESCA004050"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1919442_0_0_1"/>
    <dbReference type="OMA" id="CAFVCIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004050-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14714" checksum="5E33F96EAF70F149" modified="2013-10-16" version="1">MSLAPKEEGTTAQTALEKAEILTKGAVEFAEEKAQEMGIPTWGLVTIVILVLAIVCGLVFFCVRRFLKKRRTKDGKGKKGVDMKSVQLLGSAYKEKVQPDMEELTENAEEDEESKHSEQKLGKLQYKAQARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKP5</accession>
    <name>T1GKP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKP5"/>
    <dbReference type="STRING" id="36166.T1GKP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004072-RA">
        <property type="protein sequence ID" value="MESCA004072-PA"/>
        <property type="gene ID" value="MESCA004072"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124247_0_0_1"/>
    <dbReference type="OMA" id="IENISWE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20958">
        <property type="entry name" value="IgI_5_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000308">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF105">
        <property type="entry name" value="LACHESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="59"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004072-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17622" checksum="43E16519DFE5A1B2" modified="2013-10-16" version="1">IPDNSRFLVGQYVTIHDDVISHVNISNVKEEDGGEYTCTAQNIIGKVSHSAKVNIYGLPYIREMPRITGISGTDLTVKCPVAGYPIDKIHWERGDICTFNGTDGQTLPINRRQRSYNNGTLIIEQLQRLEDAGTYTCMAQNKQKQTARRNVEIQVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKS2</accession>
    <name>T1GKS2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKS2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004099-RA">
        <property type="protein sequence ID" value="MESCA004099-PA"/>
        <property type="gene ID" value="MESCA004099"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3415387_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004099-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="27" mass="3349" checksum="9DC645B0FCD28899" modified="2013-10-16" version="1">MKFQTNDFTEKFDLKMFHLLIQQDQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLZ2</accession>
    <name>T1GLZ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004558-RA">
        <property type="protein sequence ID" value="MESCA004558-PA"/>
        <property type="gene ID" value="MESCA004558"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_783664_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032062">
        <property type="entry name" value="DUF4803"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16061">
        <property type="entry name" value="DUF4803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588387" description="Fibrinogen C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004558-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="40828" checksum="2EB799FF32454EC3" modified="2013-10-16" version="1">MILLAILLLLTGSGGFAEGKCNLSEVKAATEVAENIIPIFKYMNPIVGIFDNDCRIDEILEVVHEISQKLDNLEILIRESTNQYKLKIEEDFKDMKTYSGDLEKFENITLRTYARNDLQETLREYHQQYFVNEGITEGTLFSQIAAFLKDKGNFEDKCDGLGVSVQQELIDYYQSIVSVEVKAIIVVHFKYYLLSNYFEIGSFTEEKNMFNSKNDHRKVEMESNFKKSLSTMTNLHFWHCYPGHIDDHTFLARVSQGVHFFEYDKISCNGELYDKEEFRMAIKNDPSHPSGFGLMYFSNAAICYSNENSERKYEWLGTNGGFFGQKTNCPTKVKNTEARNVESFFALCDVSKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMU6</accession>
    <name>T1GMU6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMU6"/>
    <dbReference type="STRING" id="36166.T1GMU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004879-RA">
        <property type="protein sequence ID" value="MESCA004879-PA"/>
        <property type="gene ID" value="MESCA004879"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015780_4_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.330:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="7">
        <location>
            <begin position="767"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="670"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="749"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="678"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004879-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="930" mass="102490" checksum="098249334FCC8BE9" modified="2013-10-16" version="1">MFSCCRCGNPKTKDGKPSKKSKQKNQKVTSDGEEEENNTTSPVPTQPQHHHLPMEAQDSSGADVVVNGQVSGTGHKPRLASPLHDEEVVVVDNNKNDSSEEQEVPQEHSEECQEEINQSKDITNGIEKGSDGREEINIAEDSPGNNSYSSSNNNSPQEDDEDDDLPSPPLPPIPCNTNSIVDIERLELNYQSTVESSPSSNATFLSSVIENRGRHFSESSGNSFPSPPPLPFPPLVPECQLSSGSSSEVLPRSCLSRRSSSLKKSVYINHSAKVIEETDRSSWEDDEVFSDSIILVKEGKDTCVLHSWPRGVPFKDPLVCQNGLMKSGLLICRSFLNRETDGSYEPPVTPVGKDELELKRHRLYSDLVNAAQAAVEHRVRFDPLGPDISGEDCDPDIRPTITSDLEHLLNRLENLVERLERSLTAKELALANQTLDSVLETRRRSFDIESAELPPIPEPNRFSLRLERIENSLGRLNDSYKRESLSENCQTHTDAEGSLENIPTVLEDFQVHEPEPIDTMSVLGYQDIVNGPLAQFLGLSHKIGGDVAQQSEFVRKAFDAQLQQINLATQISTPPPNKQQEIIKPTADQISAIQEFREKHRASPFFNHLSAISESIPALGWVCVAPTPGPYDWKEKDIIHVEWARSWIQTLTDLQQYIKQYHTTGLVWSGKGSAPSGGCPPPPPSAAGCPPPPPPPVLDMSAMSLNDASNDDRSALFAEINKGSDITKGLKKVTADMQTHKNAALRTGPAPFKAPNHHTNNINSVGPAKPIKKDPVFNKDGKKWMIEYHENNPNLVVDNTEMNNVIYVFKCVNSTLTVKGKVNNVVFDSCKKCSLLFDSVVSSIEFVNCQSVQMQVLGTVPTISIDKTDGCQMYLSSDSLGVEIVSSKSSEMNVLIPKANGEYTEQPIPEQFKTFIKGNNLQTICVESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMX9</accession>
    <name>T1GMX9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMX9"/>
    <dbReference type="STRING" id="36166.T1GMX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004914-RA">
        <property type="protein sequence ID" value="MESCA004914-PA"/>
        <property type="gene ID" value="MESCA004914"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796883_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004914-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="8007" checksum="DB1C6642A7399971" modified="2013-10-16" version="1">MAEPLEEGDLNLNEIWRTCRKIQTAVFQSNINIWKEYQNFDPNKNGLISESIFIGILGKYKNLIDYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNW4</accession>
    <name>T1GNW4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNW4"/>
    <dbReference type="STRING" id="36166.T1GNW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005275-RA">
        <property type="protein sequence ID" value="MESCA005275-PA"/>
        <property type="gene ID" value="MESCA005275"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130077_0_0_1"/>
    <dbReference type="OMA" id="CREGCER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043458">
        <property type="entry name" value="GPR158/179"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 158-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546:SF29">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005275-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13225" checksum="B577FF4B02893EF3" modified="2013-10-16" version="1">LRRGSYKCVCRKGFYYPNTSSSQKYFNGSVLEEEYEKLMLGRNSSYNVNQEYECLPCAEGCESCEDDSPCIAALNWPMRTTILVLACTVIGLLPPATWFTFRYQQVKASTMFTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNW6</accession>
    <name>T1GNW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005277-RA">
        <property type="protein sequence ID" value="MESCA005277-PA"/>
        <property type="gene ID" value="MESCA005277"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3424497_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005277-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="23" mass="2512" checksum="D8BA6C13B473CC69" modified="2013-10-16" version="1">MGKLLIALTLTLFVAVVIGQPWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP83</accession>
    <name>T1GP83_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GP83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005404-RA">
        <property type="protein sequence ID" value="MESCA005404-PA"/>
        <property type="gene ID" value="MESCA005404"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117339_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF57">
        <property type="entry name" value="RE46159P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005404-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21772" checksum="C1B00E5972877CCC" modified="2013-10-16" version="1">MASKSENYENNIVTSDELPMIFKVKYIGSEPSKGLWGIKYIRRPVDKLVAHAKQIPANKSLPNCDLTVSTSGVHIDFLDNSPFSSNTYLTDCISYGMTIXXXXXXXXLIYTRVFAMIVVKDDNSNPFEVHAFVCDTRQIARKLTYALAAAFQEYSEKIKHSKNVDNLMVNQKFAIDLRSPSELQEGMQDETEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPI0</accession>
    <name>T1GPI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Core component of the TRAPP complexes which has a function of guanine nucleotide exchange factor activity for Rab1 GTPase. Plays a role in vesicular transport from endoplasmic reticulum to Golgi and autophagy. May play a role in dendrite postsynaptic membrane trafficking.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Interacts with SDC2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
            <location evidence="9">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02094062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPI0"/>
    <dbReference type="STRING" id="36166.T1GPI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005510-RA">
        <property type="protein sequence ID" value="MESCA005510-PA"/>
        <property type="gene ID" value="MESCA005510"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053380_1_0_1"/>
    <dbReference type="OMA" id="GQRDGIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005510-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="25595" checksum="8DEC483DBAE09AE6" modified="2013-10-16" version="1">YFFSSKNYDSYGIFIVSKSGTLIFDLDYNIPRIENEKTFSYPLDLVLDYNDPKHVSVVFGQKDGISVGHVLLSVNGLSTTGQSLEDGRNIKEVLETKENYPLTLKFSRPKITTNEKISLSSVFYSLYSIASQLSPELKSSGIEVLEADTFTLHCFQTKTGVKFIVISEVGLHGVDILLRKIYELYADYALKNPFYSLEMPIRCDQFDTRLQALLEQVEKTGINNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPM1</accession>
    <name>T1GPM1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPM1"/>
    <dbReference type="STRING" id="36166.T1GPM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005555-RA">
        <property type="protein sequence ID" value="MESCA005555-PA"/>
        <property type="gene ID" value="MESCA005555"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_296405_0_0_1"/>
    <dbReference type="OMA" id="SAVFCCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000119">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000430">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000962">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="32"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="187"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="321"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="417"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="556"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="773"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005555-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1019" mass="115473" checksum="2FF364BFA4206394" modified="2013-10-16" version="1">MACSKTLNKCWSNNLFLFDGSKPEDQETGQAPKFTTDFVPIPEIAEGDIAHFDANLIPTGDQTMVIEWFYNGEVLKASHRIRTIYAFGMVALEVIGTKIEDSGTYTCRATNKYGSVEQSVTLGCVDRISGQKPRFTSHIQNLSGLKDGQSAHFEETQHEGALHRLSHLEDSSKYKRTSIIEEVVTQPPVFTMPVRDVRVAENQAVHFEARLIPVGDPKLSVEWLRNGVPIEASNRISTMHDFGYVALNMKYVNPEDSGTYTCRAMNELGQAVTSATLIVQTKASLQMETQNEMALQKIHQLEDHTKYQRTEEDEYEVTQPPRFVARLNGPASRHEGQSAHYECRIEPYPDPNMQVEWFHNGKPLQTGHRFRTTYDFGFAALNILTVYAEDSGEYTCKVTNRLGTASSSIKLQVQSKPQFGRPLRNVDVPEGRPVHLEATLTPVNDPTMQVEWFCNGKPILTGHRFKTTYDFGFVALDILYAHAEDSGTYMCKAKNAVGEAVTTSAVNVTANARIYHETLDAQRLEKIQSMQQIQQLEDYSRYQRQESSEEIFNQKPNFIRPLRDLGSFEEGKNVHFEAQLTPVNDPSMRVEWYKDGRPITASSRITAIFNFGYVSLNIMHLRAEDAGSYTVRAVNKMGEAISTSNIQVVSKSQVTGDLGIPEQQRYIEKVEQLEHYQKAQHQKYTQEIPESRQAPEFKTQIQNQIDLREGGYAHFEARLEPIGDSTLRVEWLKDANARIYHETLDAQRLEKIRQLESYEAPKKIEVEDKGQKPVFLTPLTSLEHLKEGEHAHLECRVEPINDPKLKIEWFFNGKAIMAGHRFRTTHDFGYVALDVLYVYGEDSGTYMCKATNLLGEAVNTCSIRVLNRRSMILDSQHPDALEKIQKLESRVVSTRSEVEDLPISPPSFSVQLRGTTEIYEGESAHFEAQVQPVHDPNLRIEFYRNGKPLPSASRYHITFDFGYIALDIQKAVAEDSGEYSVKAFNALGQCVSSTNLRVLTRGTIIADTQHPEGLEKIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPU4</accession>
    <name>T1GPU4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Timeless N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPU4"/>
    <dbReference type="STRING" id="36166.T1GPU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005638-RA">
        <property type="protein sequence ID" value="MESCA005638-PA"/>
        <property type="gene ID" value="MESCA005638"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177366_0_0_1"/>
    <dbReference type="OMA" id="VSYLHAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009649">
        <property type="term" value="P:entrainment of circadian clock"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005638-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12998" checksum="B9E1C5BE4E62D7DC" modified="2013-10-16" version="1">HLIWILRRDLDNHEYRRHIGRAKVLQTDLVPMLPEYIENEEFSDVLIRLLVVLTNPTLLLYRDGAPKDNHGRKVFLELIDILQGYKEAFTKDRIWAALAMRLHKVLNVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPV7</accession>
    <name>T1GPV7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPV7"/>
    <dbReference type="STRING" id="36166.T1GPV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005652-RA">
        <property type="protein sequence ID" value="MESCA005652-PA"/>
        <property type="gene ID" value="MESCA005652"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_954059_0_0_1"/>
    <dbReference type="OMA" id="CAYLFAE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF96">
        <property type="entry name" value="LP14756P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005652-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="292" mass="32105" checksum="90DD71EC64D20D11" modified="2013-10-16" version="1">MDLGWRACYYIAGICTLIVAVITGVTLKEPERKAIGEDSDKSNTEGMSLTKVMLHPRMILLMLAASLRHCGGMTFAYNADLYYNTYFPEVDLGWWLFALTIGIGSIGVVVGGVVSDKIVAKMGIRSRGYVLALSQLISTVPAFGSVYFNPTWAMITLALSYFFAEMWFGILFAIVVEIVPLKMRSSVIGIFLFVMNNIGGNLPIAVDPVAKRLGYREALMIFYAGMYGISSILFLVTCIFLESGKKEKTETGGNIAENAYQNSGFTNDEGPKVIYTPRILGAQDLDKESIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR36</accession>
    <name>T1GR36_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006108-RA">
        <property type="protein sequence ID" value="MESCA006108-PA"/>
        <property type="gene ID" value="MESCA006108"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1857564_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006108-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="16024" checksum="27EAB16A716967D3" modified="2013-10-16" version="1">MYAVREFNIEPFLNVENTLDSKIHKRRNISYNDNKPDKPKSYPKTTRSILTITYVKVPVLVENSKSHSYQNLENITKVCENLLQVCSEVCISRKIFKELGARNATKKRLRIHLDKKKVSIFSAFQRPPHKKCGLPHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR97</accession>
    <name>T1GR97_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANT/ATPSC lysine N-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02145914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02145915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR97"/>
    <dbReference type="STRING" id="36166.T1GR97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006175-RA">
        <property type="protein sequence ID" value="MESCA006175-PA"/>
        <property type="gene ID" value="MESCA006175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068443_4_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905706">
        <property type="term" value="P:regulation of mitochondrial ATP synthesis coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026170">
        <property type="entry name" value="FAM173A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610:SF9">
        <property type="entry name" value="FI06469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20183" checksum="E0E566A895443183" modified="2013-10-16" version="1">MDFIETSNTQSVSPASQKKLSKTATTLIALTGGIGIGLTVVCASFVAPAFRKYCLPYVPATDQQIKNVLSVIPKNIQGKLLDIGSGDGRIFDIKSYDYIVIFGVEQMMNDLEHKLIAEAKGNSKVIACRFPLPNSEPIKVIGDGVDTVWLYDLENSNKSKILELVKVLILQGSFRKTFKIQKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRG3</accession>
    <name>T1GRG3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006247-RA">
        <property type="protein sequence ID" value="MESCA006247-PA"/>
        <property type="gene ID" value="MESCA006247"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530040_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006247-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9745" checksum="CB5C19E84C9C2A4C" modified="2013-10-16" version="1">MLAHQFKHDINFLHQFQPKAGLKKDYRRFIDWTTPNSEKDSEIAPIPGLVRDMHTLWALFLRGGGLPPHKLDSAQSQHKIIMID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRU8</accession>
    <name>T1GRU8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRU8"/>
    <dbReference type="STRING" id="36166.T1GRU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006391-RA">
        <property type="protein sequence ID" value="MESCA006391-PA"/>
        <property type="gene ID" value="MESCA006391"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309211_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588496" evidence="1">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006391-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11601" checksum="20C9EA5E0D4AE387" modified="2013-10-16" version="1">MFPNTFLVGFFLLYFGIFSGSNGRFNDKSEIFSFPEYAYKQTNKNELTYREFEVACENSKSCLDLEGVDKVKCQGNVFHKSVTRTFMLSMSLKKEKSMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS07</accession>
    <name>T1GS07_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02086192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS07"/>
    <dbReference type="STRING" id="36166.T1GS07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006454-RA">
        <property type="protein sequence ID" value="MESCA006454-PA"/>
        <property type="gene ID" value="MESCA006454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_797626_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="348" mass="38983" checksum="8219CAE2003A81AA" modified="2013-10-16" version="1">MQKLVWSDELSHVAELNAQTCTMTHDCPASPSFLQASQNLASRKFTSPPGGREILIKLIAPWFYEYFRADLSYIDNSIEKLTRNYKKSDFLKMKNLKCPGINTDGSYTTGHYGTFVQDTLTHIGCAFYKCGPIINKNGKEAETFYLVCNYDRTVTSSSKLYETSDIGGSKCSKKSSKYCGLCLDKEEEDTGPCHEVTYTLPDWFGQNDDEDDEDTAGGDSIDSIPTEAFQPPESFCSKVENICGDTRHLHCGGSLVPKEIIGNNPNNIELTPILKLYILKKLNELRNIIACGEPKVTNVAGDTFPMAAKMPKLSWDNELEWLADLNAKTCLFNMIVLFLKNTHQLVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSE2</accession>
    <name>T1GSE2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149281">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006597-RA">
        <property type="protein sequence ID" value="MESCA006597-PA"/>
        <property type="gene ID" value="MESCA006597"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624829_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9087" checksum="9542F89C19C70BDB" modified="2013-10-16" version="1">MRTLTKYSNLDPSQKCPIKPVNETYSSKLSTGYGQTRNLPLIVGKYKLASKAYIEDKHKMIEIFNLNIYVDFFENNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSK0</accession>
    <name>T1GSK0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BCAS3 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131236">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSK0"/>
    <dbReference type="STRING" id="36166.T1GSK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006660-RA">
        <property type="protein sequence ID" value="MESCA006660-PA"/>
        <property type="gene ID" value="MESCA006660"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1464811_0_0_1"/>
    <dbReference type="OMA" id="DKHENMP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045142">
        <property type="entry name" value="BCAS3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048382">
        <property type="entry name" value="BCAS3_WD40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13268:SF0">
        <property type="entry name" value="BCAS3 MICROTUBULE ASSOCIATED CELL MIGRATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13268">
        <property type="entry name" value="BREAST CARCINOMA AMPLIFIED SEQUENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21034">
        <property type="entry name" value="BCAS3_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCAS3 WD40" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006660-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="20138" checksum="11E4B32E42991AA3" modified="2013-10-16" version="1">GDTSAKVQNISFSLDTRWVAVSTLRGTTHVFPITPYGGQPTARTHSNLHVVNKLSSFHHSAGLTADGRSSSPISHTDTTNFSQQLMPYFNQTMPPFPRPTFVLPLAQLRQPFTLSNNAPASAAAPKTERGNRQRLSSLSDDNGKPLSVCAVFAKSRSWLLDPPNSTRDGPSHKLQRKAVDSLFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT33</accession>
    <name>T1GT33_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Angiotensin-converting enzyme</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT33"/>
    <dbReference type="STRING" id="36166.T1GT33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006853-RA">
        <property type="protein sequence ID" value="MESCA006853-PA"/>
        <property type="gene ID" value="MESCA006853"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150223_0_0_1"/>
    <dbReference type="OMA" id="MCTASRQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008241">
        <property type="term" value="F:peptidyl-dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001548">
        <property type="entry name" value="Peptidase_M2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514:SF44">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6 7">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006853-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16630" checksum="69C1A74E58E86F08" modified="2013-10-16" version="1">MRSILGGVEPAVQRSDAGFDPTAKYHIGANIEYLRYFAARVFQCQFHKALCIKARQFDESDPKSFLDNCDIYGSTEAGEVFKEFLSVGSSKNWRVVLEKFTGDRKMSAKALLDYFKPLRKWLQGEIKRLDITTGWQISDNAVGSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTA3</accession>
    <name>T1GTA3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006928-RA">
        <property type="protein sequence ID" value="MESCA006928-PA"/>
        <property type="gene ID" value="MESCA006928"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075165_1_0_1"/>
    <dbReference type="OMA" id="CKEPTHK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588489" description="Cuticle protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006928-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="18742" checksum="B286D35CFC8EC0E0" modified="2013-10-16" version="1">MKYIFLLQIIVALAFVAFASAGIIPAAQVYHSAPVLAAAPVVHAAPLVKAVAQPILAKQVEEYDPHPQYKFGYDVQDALTGDSKSQFEAPAVVAAPAPVVKTIAPVAYTAAHPVAYSAPIVKTVAAHPVAYTAPVVKTVAHHPVAYTSAHPVAYTAAHPVAYSSPIVKTVASAPVLAYHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUG3</accession>
    <name>T1GUG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02127956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02127957">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUG3"/>
    <dbReference type="STRING" id="36166.T1GUG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007376-RA">
        <property type="protein sequence ID" value="MESCA007376-PA"/>
        <property type="gene ID" value="MESCA007376"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011711_5_3_1"/>
    <dbReference type="OMA" id="CIVNSHF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15663" checksum="21E1EE4934C20D84" modified="2013-10-16" version="1">MQGLLLTIFIKKEHLLHLRNIEAEYTRTGLGNLWGNKGAVSVRMQLYGVATVFVVAHLAAHDHELDERIEDYKQIYKSHHYHVKKYKNIFDHDYVFWFGDLNFRLAGNDSPEEVRALVEENKLQELLERDQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUQ8</accession>
    <name>T1GUQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUQ8"/>
    <dbReference type="STRING" id="36166.T1GUQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007476-RA">
        <property type="protein sequence ID" value="MESCA007476-PA"/>
        <property type="gene ID" value="MESCA007476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840127_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001507">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="29"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="15644" checksum="464FAF5B1B220E59" modified="2013-10-16" version="1">MDCDYYHSNAYHVNGSLKITIIFLSAPDPPSNLSVQVRSGKTATISWSPPSQGNFTSFKIKILGLSDGYETNRTITVEDGALSYPLKELAPGSTYQVQAYTIYDGKESVAYTSRNFTTMKRAPFNVRARHKDLLDIKILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUV1</accession>
    <name>T1GUV1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02156591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUV1"/>
    <dbReference type="STRING" id="36166.T1GUV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007522-RA">
        <property type="protein sequence ID" value="MESCA007522-PA"/>
        <property type="gene ID" value="MESCA007522"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1201029_0_0_1"/>
    <dbReference type="OMA" id="EHTDPRP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019152">
        <property type="entry name" value="DUF2046"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15276:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15276">
        <property type="entry name" value="H4 D10S170 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09755">
        <property type="entry name" value="DUF2046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="187"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007522-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26186" checksum="FF03CD55B1CDF2E0" modified="2013-10-16" version="1">MRKIEKLEHETLTKQSNLEQLRREKVELENTLEQEQEALVNKLWKRMDKLETEKRSLQKKLDQPVSDPTTPKDISNANGDTATNISTHIQTLRSEVIRLRANLSAAQKSNNEKEQQFAQEEKSIREENIRLQRKLQLEVERREALCRHLSESESSLEMDEERFYNENRFAGGSSGLSAAANRQRTISSPSPHSPSNSRPLSPGSAATLGVGSQSRCCTCQLPILGVSTYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV53</accession>
    <name>T1GV53_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tripeptidyl peptidase II Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV53"/>
    <dbReference type="STRING" id="36166.T1GV53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007635-RA">
        <property type="protein sequence ID" value="MESCA007635-PA"/>
        <property type="gene ID" value="MESCA007635"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036331_0_0_1"/>
    <dbReference type="OMA" id="XINILLI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022232">
        <property type="entry name" value="TPPII_C_art"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046939">
        <property type="entry name" value="TPPII_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022229">
        <property type="entry name" value="TPPII_Ig-like-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12580">
        <property type="entry name" value="TPPII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12583">
        <property type="entry name" value="TPPII_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tripeptidyl peptidase II second Ig-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Tripeptidyl peptidase II C-terminal" evidence="3">
        <location>
            <begin position="276"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007635-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="546" mass="60323" checksum="4935DD7726152E15" modified="2013-10-16" version="1">MMHLNGVHVLDIISLGAVDVHPIISLKQAVVPLRPTEAKITPLSPFRDVLPDGRQIYQNCLVYNLHLTKSAEVSIGASVLSHVLYESQFESQLWMLFDSNKMLVACGDAYSLGSSTIKLEKGDYTIRLQVRHERKELLEKVNDTALLIQHKLSSNLALDAVIGGKKSTTVHLNGATKPKCFYVAALANEKINKANLPAGCTYLNGAIQLAKEELGRVASSRRFKYVIGEQLEQTNKQSGSAAATASDAGSNAANASAGGKSDNKTTSDGSPNKGGKNNKSKGNSYDDYLEGIRDYKCSMISKLGDLSKAEEIYNEVVSEYPKHLSVHMNLIQNLESTVPIKTVYPFSFDNLVRSQSSATASEAVKKVDLEKLSTVLKRTIELTDIVIKETDKDALLAFYGMKTDTRSDASKIKSTMDKQKNTLLEAYAKNGIAKCKLAILEQGHSDEVKTTLSSNLIEQIDSNLVEIQKFVDINDSKVLQFSIWHAFSNRQFGRLAKFLLKLYDEKLSKDVLEELSLIAKEKEWKHLSEQIERQIVSANPGGFRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVD6</accession>
    <name>T1GVD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVD6"/>
    <dbReference type="STRING" id="36166.T1GVD6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007732-RA">
        <property type="protein sequence ID" value="MESCA007732-PA"/>
        <property type="gene ID" value="MESCA007732"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_991399_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007732-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="281" mass="33109" checksum="A562B86BC638694C" modified="2013-10-16" version="1">MRKILKFCSISATKKEIPEDDLKEITVNTQAKKSMFENGDIFRKSKIERTDSCDIEVIENGLGKKSRNIFKEMEVENHTNNVSNFTKPKVSIPKKEVEESKEEQKPADIVRSSRSPSPELDISSKNVSSAYEFFSTLSTKKEIPEEKKRKEIGIGKSQTTTKMLSRFRELEQKKKRETGRRYRSSDSDSEEEDSESEEDRMQREEDRDLIEARQLARAKHLREKFEKWDNAEQSFERERENENIESTPLESTKSLREKFEHFGEQKSLEEKRIKHKITRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWG0</accession>
    <name>T1GWG0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008137-RA">
        <property type="protein sequence ID" value="MESCA008137-PA"/>
        <property type="gene ID" value="MESCA008137"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1379554_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008137-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22509" checksum="2A5B905D2D426F2E" modified="2013-10-16" version="1">MMYEYVARPIRSALKRRIDTVQPYYYEKQTDIDFSTEHETTTPDETGITFPEDNEGDGLFSGSVQTQSTDESANAPSEDSDSESNGNRANDGYQEAIPGPVTRLVVLANRGLANLVQDLILVSLFCLFFVVDEFNGEKDFNWGFESKYKIVWSLKLFESEPEKICERFMEISKIEIFRIAATSERLVNFKAKVITALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWI9</accession>
    <name>T1GWI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein MAK10 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWI9"/>
    <dbReference type="STRING" id="36166.T1GWI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008166-RA">
        <property type="protein sequence ID" value="MESCA008166-PA"/>
        <property type="gene ID" value="MESCA008166"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054634_0_0_1"/>
    <dbReference type="OMA" id="FAECEYA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="2">
        <location>
            <begin position="4"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008166-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="304" mass="36033" checksum="26FFDFC1A03D2403" modified="2013-10-16" version="1">MNMHTFVYFLQICGFNRARQRDKFARLIEDGFVNVQEEAARLDAHFNAIAVKGDSYNPHMAYFGTWNLYHCLKAMSLYLLSGFELELYSVHEYLYIFWYLYEYLFGFLITALKRAESIVIEQEQLDMHHKNNLNAQSKQRKPKIKKHRKNGIPFRQEIFLNTAYQSICGGYYKAIGAFTKEEKIRQPLQIFDSEYIRFNHRFAPFATLTSPPPIPYHEFDMMRKHLLRSNANDLYISAATHFHEARLNLEYIQNPDQEILQMIQVAKVNYVVMSLLAKGHKRDSKSQPVFDYSQHRYFPVIKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWN9</accession>
    <name>T1GWN9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02025721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWN9"/>
    <dbReference type="STRING" id="36166.T1GWN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008217-RA">
        <property type="protein sequence ID" value="MESCA008217-PA"/>
        <property type="gene ID" value="MESCA008217"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457339_0_0_1"/>
    <dbReference type="OMA" id="FIKNCIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF2">
        <property type="entry name" value="RE48874P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008217-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="9657" checksum="31FE9F800E379CD9" modified="2013-10-16" version="1">MCIICIGINAFMIVWMLTALGVVIHCPDIVMGLTFLAAGSATPEAVSSAISVRKGDSGIGVSNSLGANSLAILLSLGLPWFIKNCITFNSEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX15</accession>
    <name>T1GX15_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC-2 type transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX15"/>
    <dbReference type="STRING" id="36166.T1GX15"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008353-RA">
        <property type="protein sequence ID" value="MESCA008353-PA"/>
        <property type="gene ID" value="MESCA008353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152079_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF105">
        <property type="entry name" value="FI02074P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="7">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12839" checksum="11FDDF9D99C71DBC" modified="2013-10-16" version="1">MFDHLKFCVGCILVIVYTQVMTPILSYSMEVKLVKKEYFNRWYSLTAYYMALTVSRIPLQIFFNIVFLSLVYYLAGLPPQLWRFCLFSLAGLMVSFAAEGFGLAIGATFSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX36</accession>
    <name>T1GX36_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX36"/>
    <dbReference type="STRING" id="36166.T1GX36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008375-RA">
        <property type="protein sequence ID" value="MESCA008375-PA"/>
        <property type="gene ID" value="MESCA008375"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_1_7_1"/>
    <dbReference type="OMA" id="CVICIIA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577727" description="Peptidase S1 domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="32"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008375-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="19256" checksum="C286D643D19720C8" modified="2013-10-16" version="1">MLKIVGIFCVICIIASVSSQPLDVIDGRRPPIVGGYEIDIERTPYQISLRRRGRHICGGSIYSDHIVITAGHCIYGTANSRDLSVVAGATLINSNVGQEFRVARFILHPKYKDLNKDYDVAIFVLNGRFQFNKVVQPIPLAKEMAPEGSNVFISGWGYLDVNIPITPNHLMATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXA8</accession>
    <name>T1GXA8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008452-RA">
        <property type="protein sequence ID" value="MESCA008452-PA"/>
        <property type="gene ID" value="MESCA008452"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485900_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9968" checksum="8A559D80C81023C0" modified="2013-10-16" version="1">MDYGDSLRDLDFWGGGKYVFAEINRLLVEAINDFNVAADLSVWLALKIYELVEKRSRGGTLTNTRTGQMLIIMEFTLGLDKDWYRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXB0</accession>
    <name>T1GXB0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02383465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXB0"/>
    <dbReference type="STRING" id="36166.T1GXB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008454-RA">
        <property type="protein sequence ID" value="MESCA008454-PA"/>
        <property type="gene ID" value="MESCA008454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256777_0_0_1"/>
    <dbReference type="OMA" id="NYPEFEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.540">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027378">
        <property type="entry name" value="Nucleotide_channel_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF280">
        <property type="entry name" value="FI21844P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11838" checksum="E305B216A9719418" modified="2013-10-16" version="1">PLKVKSVIGYRHVQVLLLFGATIIEYYGRINISMALVAMTDAKAANPNYPEFEWTTKEKSLILSSFMWSYILTQFPGGYLSHRYGSKLVLTVAVFERPIVYVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXC3</accession>
    <name>T1GXC3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008469-RA">
        <property type="protein sequence ID" value="MESCA008469-PA"/>
        <property type="gene ID" value="MESCA008469"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3280065_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008469-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="4753" checksum="D6D8AF390310E689" modified="2013-10-16" version="1">MVEKFQKVESAVAVVILKMFLMVASFAKESRRLLRRRLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXU9</accession>
    <name>T1GXU9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2 S</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E2 ubiquitin-conjugating enzyme S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin carrier protein S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin-protein ligase S</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02148752">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXU9"/>
    <dbReference type="STRING" id="36166.T1GXU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008658-RA">
        <property type="protein sequence ID" value="MESCA008658-PA"/>
        <property type="gene ID" value="MESCA008658"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_5_3_1"/>
    <dbReference type="OMA" id="QPAKCGA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000034">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="17">
        <location>
            <begin position="13"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="159"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="14">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="211" mass="23367" checksum="00E9C839537B6E8E" modified="2013-10-16" version="1">MNSISNVENLSPQTIRHVLREIQGLVESPLEGIKIQLNDADVTDIHAIIDGPAGTPYQSGSFKVKLSLGKDFPQTPPKAYFITKIFHPNVAASGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKLLLERYDDYSQRAKMMTEIHAQNVKSDSKDGDGEEEGDGADGGPLSKKSAIDKKVQDKKKEKILKEKKRTLKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYA2</accession>
    <name>T1GYA2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-synthesizing GTPase</fullName>
            <ecNumber evidence="1">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02176639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYA2"/>
    <dbReference type="STRING" id="36166.T1GYA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008825-RA">
        <property type="protein sequence ID" value="MESCA008825-PA"/>
        <property type="gene ID" value="MESCA008825"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027154_1_1_1"/>
    <dbReference type="OMA" id="EHYDLQY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008825-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="31357" checksum="A1218CCF48288F01" modified="2013-10-16" version="1">MSESYANAKIFKCDNPKCTRPACFTSGGSSKDDSFPCLRPACSGRFQLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHIIILQNKIDLVKESQAKEQYEQIVKFVQGTVAEGAPIIPISAQLKYNIEVLCEYICKKIPIPQRVFTTPPRLIVIRSFDVNKPGCEVNDLKGGVAGGSVLSGVLKVGQEIEVRPGVVTKDSTGKVTCRPIFSRIVSLYTEQNELQFAVPGGLIGVGTKIDPTLCRADRLVGQVLGAVGELPEIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYH7</accession>
    <name>T1GYH7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02127122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYH7"/>
    <dbReference type="STRING" id="36166.T1GYH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008905-RA">
        <property type="protein sequence ID" value="MESCA008905-PA"/>
        <property type="gene ID" value="MESCA008905"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_3_1"/>
    <dbReference type="OMA" id="YLEFPAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000054">
        <property type="entry name" value="Complement C1r subcomponent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008905-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8467" checksum="099D75E52111AB0C" modified="2013-10-16" version="1">MFCAGHRRGRIDTCAGDSGGPLLCRDSTKPNHPWTIFGITSFGDGCAKRNKFGIYAKVPNYVDWIWSVINCNGNSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYY1</accession>
    <name>T1GYY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYY1"/>
    <dbReference type="STRING" id="36166.T1GYY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009069-RA">
        <property type="protein sequence ID" value="MESCA009069-PA"/>
        <property type="gene ID" value="MESCA009069"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_0_1_1"/>
    <dbReference type="OMA" id="LCVFINR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="7">
        <location>
            <begin position="3"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009069-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="274" mass="31765" checksum="2FC055B3F111B35D" modified="2013-10-16" version="1">YILADLGYDVWLGNARGNRYSKGHNTLSVESEAFWDFSWHEIGYYDLPAMINYVLNTTNNDKLHYIGHSQGCTSFFVMCATRPEFNNKIISMQALAPAVYMSETKEHPYLRIVELYLKNPVITRNKGLLNGEFKQICKMSSDTLKTCMEAIFSVIGRNKKEFNLKMFPVVLGHYPAGSSAKQIRHFLQIINTGRFAEYDYGTSENLFKYRQFLPPIYNMTRVTAPIYIHYSANDLLVNQIDVEHLYEDLVNPMGKYLVPMKEFNHMDFLWAINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ25</accession>
    <name>T1GZ25_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009116-RA">
        <property type="protein sequence ID" value="MESCA009116-PA"/>
        <property type="gene ID" value="MESCA009116"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349047_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009116-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11064" checksum="022D51323BA4E4FA" modified="2013-10-16" version="1">MNDIFLWFCSRNAINIAEWFTNNPNIESTSSQYMGSHDRLVNKKRKSSHLPPLPPPTCFDIWLLKSPIVVKLLAPRNLFAGFLVESCCIELTLRAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZJ3</accession>
    <name>T1GZJ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009296-RA">
        <property type="protein sequence ID" value="MESCA009296-PA"/>
        <property type="gene ID" value="MESCA009296"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3002473_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009296-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="57" mass="6199" checksum="D47759EECD5F3AD1" modified="2013-10-16" version="1">MAMVTIAMEVPGMITMAVIVVEIMVTEAMTTPDHPVDMAMVNTIVILISKPPLLIHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H027</accession>
    <name>T1H027_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02144878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H027"/>
    <dbReference type="STRING" id="36166.T1H027"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009491-RA">
        <property type="protein sequence ID" value="MESCA009491-PA"/>
        <property type="gene ID" value="MESCA009491"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870277_0_0_1"/>
    <dbReference type="OMA" id="HEWFLAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000001">
        <property type="entry name" value="Maltase A3, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF234">
        <property type="entry name" value="MALTASE A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="7">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009491-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="16324" checksum="13C394B1F7D78280" modified="2013-10-16" version="1">MADFGYDIADYYQIDPIFGTMADFDSLIAKSKEVGVRIILDFVPNHSSDEHEWFKKSAAKDPEYKDFYVWHPGKMIDGKRHPPSNWISVFRHSAWTWHEGRQEYYLHQFLSKQPDLNFRNPKVREALKDILKFWLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0D8</accession>
    <name>T1H0D8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0D8"/>
    <dbReference type="STRING" id="36166.T1H0D8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009613-RA">
        <property type="protein sequence ID" value="MESCA009613-PA"/>
        <property type="gene ID" value="MESCA009613"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1435959_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="22400" checksum="66FF41AC9F4E9D19" modified="2013-10-16" version="1">MTLNLDFIKTIEEDAEVENLSDDSDAEVEYQPTKLRHKKLTEFEKGFEFVSSAKEYNKDTWDDLMKYVKRKARTKTDDKIAAKIKDRGDEDQEPNDESDDEIDLSEDELKHDNLRIREKKLKKKKKKGDQDEEDLKVEDEEADKEFFEEAENSEQITSFYQMNLSRPLMRAIGVMGSYIQHQFKQPQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Q6</accession>
    <name>T1H0Q6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation elongation factor EFG/EF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Q6"/>
    <dbReference type="STRING" id="36166.T1H0Q6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009741-RA">
        <property type="protein sequence ID" value="MESCA009741-PA"/>
        <property type="gene ID" value="MESCA009741"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237707_0_0_1"/>
    <dbReference type="OMA" id="ICMNNDE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EFG/EF2" evidence="3">
        <location>
            <begin position="64"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009741-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="24960" checksum="883F38C507BC6B01" modified="2013-10-16" version="1">MHDLRKMYSEIDIKVADPVVAFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENETVSINWNKKKIGEFFQVNYDWDLLAARSIWAFGPDTTGPNILVDDTLPSEVDKSLLTSVKDSIVQGFQWGTREGPLCEEPIRNVKFKILDAVIANEALHRGGGQVIPTARRVAYSAFLMATPRLMEPYNFVEVQAPADCVSAVYTVLARRRGHVTQDAPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0S9</accession>
    <name>T1H0S9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-ribosylation factor-like protein 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0S9"/>
    <dbReference type="STRING" id="36166.T1H0S9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009768-RA">
        <property type="protein sequence ID" value="MESCA009768-PA"/>
        <property type="gene ID" value="MESCA009768"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_14_0_1"/>
    <dbReference type="OMA" id="ETTFSMP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46688">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46688:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009768-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="20104" checksum="328EBACB230809CB" modified="2013-10-16" version="1">MSCLCLGPKRSGKTHLLKALQEEDSIDHTSYSKPTVGTNIFDIKFITKREQNNEKFSKPVPTQTKVQLARRKNLPKTIKILEIGGDMAPLWRQFFGNVNKIVYVVDTSNLCQISAAGVLLYSILAEPRLQRTKFLLVLTKMDYAYRQMRNEALLMLNMVKLQNEIRQNITIVEASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0U4</accession>
    <name>T1H0U4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02103458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103459">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0U4"/>
    <dbReference type="STRING" id="36166.T1H0U4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009786-RA">
        <property type="protein sequence ID" value="MESCA009786-PA"/>
        <property type="gene ID" value="MESCA009786"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987157_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009786-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14604" checksum="3C50FCB795CBB42D" modified="2013-10-16" version="1">ERYDFVINADQQVGAYWIQLRGLGECGIRRAQQLAILRYARGPYQPSSPAPTYDVGIPQGVVMNPLDAQCNRQRDDAICVSQLKSAKDIDRGILQEKPDVKIFLPFRFYLYRPEELFMPNTYNRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1G4</accession>
    <name>T1H1G4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1G4"/>
    <dbReference type="STRING" id="36166.T1H1G4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010025-RA">
        <property type="protein sequence ID" value="MESCA010025-PA"/>
        <property type="gene ID" value="MESCA010025"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_526081_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097638">
        <property type="term" value="P:L-arginine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF105">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="3">
        <location>
            <begin position="32"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010025-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="518" mass="58331" checksum="16741775D573742D" modified="2013-10-16" version="1">MGPLLVSIFTATCLLILLSITVQPREKGNNLFRVPWVPLVPAISIFVNVYLMLQLDYYTWIRFGFWMIVGLPMYFVCVCMYDRPSNSKKSMKTSVGCSNGTLTTLVYPEELNRDINGLTQPEMIEIKKDEIEEEPYFSKLSNGISIINVSDEIEKEPAISRLSNGVSVINVEDEIFESATDDEKSVIAMLDDVIQHEEELSSPPELDRSMSADSSLEPSLIQSSTVAIVHIEDQPEEQENRLQNLPITQTINETLVEEKEITEDEARQAAEEVIEQILTSPEILKVLEQNNENINDSRKIRLISTPHIPAPASKAVEEKKESTNNDSESSMNPLRPSKSAEDVRKLLFAAINKTKPDDDEVEEKGGIPKPPKFDPVLYKTINSPYNLESKTAVKKRPSIKEIIVSKESSPVKESSPEEETETPAPVFRAKLEEILKRGPSHKSASRQSYMEANGIRNPVIRRKSSKKDDNYKEILRTIRANKVLSAEKTRSDRNNNSVTNGQTSLLEDLRKSLKPVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1U2</accession>
    <name>T1H1U2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1U2"/>
    <dbReference type="STRING" id="36166.T1H1U2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010162-RA">
        <property type="protein sequence ID" value="MESCA010162-PA"/>
        <property type="gene ID" value="MESCA010162"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661602_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF77">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Receptor ligand binding region" evidence="9">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010162-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="9131" checksum="E1530DFF65F6226E" modified="2013-10-16" version="1">MEEGIEIVTRQSFLSDPSDAIKNLRRQDARIIVGLFYVVAARRVLCEMFKQQLYGRAHVWFFIGWYEDNWYEVNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2B3</accession>
    <name>T1H2B3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor subunit alpha homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Docking protein alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02169665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2B3"/>
    <dbReference type="STRING" id="36166.T1H2B3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010352-RA">
        <property type="protein sequence ID" value="MESCA010352-PA"/>
        <property type="gene ID" value="MESCA010352"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476792_0_0_1"/>
    <dbReference type="OMA" id="KNHSAPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000566">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="8"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="11">
        <location>
            <begin position="9"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20041" checksum="FB53D3428773C89E" modified="2013-10-16" version="1">MEAKRSSRPYVMAFCGVNGVGKSTNLAKICFWLIENNFSVLIAACDTFRAGAVEQLRTHTRHLNALHPPQRHGGRTMVNLYEKGYGKDAAGIALEAIKHASDTGIDVVLIDTAGRMQDNEPLMRALAKLIKVNDPDLVLFVGEALVGNEAVDQLTKFNQALADYSSNENPHDTKNHSAPQIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2G5</accession>
    <name>T1H2G5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2G5"/>
    <dbReference type="STRING" id="36166.T1H2G5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010410-RA">
        <property type="protein sequence ID" value="MESCA010410-PA"/>
        <property type="gene ID" value="MESCA010410"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1405726_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933">
        <property type="entry name" value="F-BOX ONLY PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933:SF3">
        <property type="entry name" value="F-BOX ONLY PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010410-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21858" checksum="A3E78CC24E387AAE" modified="2013-10-16" version="1">GFKTNSVGLAPPGGTFSDQDYVDVEKAFVDPLKLFLSRKRPSVKVLDLGAIEALTYHGTDFLKLLNRPQDLQEITLASIKFDPSHYPILAVEHVFFEKCTSLQVLSLDYDTLNEDILKTIQILPLKTFMICVHGLDLSHSGISETAWTEFSNHFKTIKLVLTLIYAYEAVEILQMNLLRRNMPITHLGSFSVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2N7</accession>
    <name>T1H2N7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2N7"/>
    <dbReference type="STRING" id="36166.T1H2N7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010490-RA">
        <property type="protein sequence ID" value="MESCA010490-PA"/>
        <property type="gene ID" value="MESCA010490"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_633549_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016011">
        <property type="term" value="C:dystroglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021675">
        <property type="term" value="P:nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008465">
        <property type="entry name" value="DAG1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030398">
        <property type="entry name" value="SEA_DG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21559:SF21">
        <property type="entry name" value="DYSTROGLYCAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21559">
        <property type="entry name" value="DYSTROGLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05454">
        <property type="entry name" value="DAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51699">
        <property type="entry name" value="SEA_DG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S72" evidence="3">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S72" evidence="3">
        <location>
            <begin position="192"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010490-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="433" mass="49265" checksum="720EC4D5F68AD6A7" modified="2013-10-16" version="1">MSISFFVAISDTVSEQTFSTIVVRGINYDKENPKRVYFKFTNETLTKEECPQKKLDQLIAFLFTSLPNKLNPSRMQIQAIDGQLIGTCKSTIEIPGTSITPISMKNYPPVPRNQVDRLNATVGHLMVFKVPIDTFYDPEDSTNLKLFLQTEDNKNISEKNCDKGSEKYNLVAEDKEGQQATDAMVVEIENAPRHDYPVYIKILLGIKSDDFKLIAQRKFIEKIAAIFGDSTTNNIQIRSINPTPEFNETTVTFYNTTLHKVQHQCPEERISYVRNVLLHHDNIIKNHVRKAFEKDFRLKTVSIIGACHPNQNMFNTGKMPITNRYPSSNNPSSTSTTSSNLSDDYLYSFIIPAIIIISMILLASIVACILHRRKLTGKMEIGDEEEKKSFRSKGIPVIFQDELEEKPDIGNKSPIILKYEKPPLLPHNNKEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2S9</accession>
    <name>T1H2S9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Yellow-c</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major royal jelly protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02158317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2S9"/>
    <dbReference type="STRING" id="36166.T1H2S9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010533-RA">
        <property type="protein sequence ID" value="MESCA010533-PA"/>
        <property type="gene ID" value="MESCA010533"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031076_2_2_1"/>
    <dbReference type="OMA" id="CSNAFAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="MRJP/yellow-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PROTEIN YELLOW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009:SF11">
        <property type="entry name" value="RH54244P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010533-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25007" checksum="114F84D5352C94AD" modified="2013-10-16" version="1">YEIPKEQTKEDTFLANVIVDTDKNDCSNAFAYVPDLGGYGLIVYSLKENRSWRVKHNFFHFDPLMGDFNVAGVRFQWTDGIFALAVGNKKPDQSKDVYFHALASTKEFVVSNRVLQNETYANSPEAYNDYMLVGDRGHSGQSTAEVFDEKNNVIFYTQINKDAIGCWNTQKPLNPDTQGLVDSDSTALVFPNDMKLDRERNLWVLSDKMPLYVYKSLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2W4</accession>
    <name>T1H2W4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131357">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2W4"/>
    <dbReference type="STRING" id="36166.T1H2W4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010570-RA">
        <property type="protein sequence ID" value="MESCA010570-PA"/>
        <property type="gene ID" value="MESCA010570"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148710_0_0_1"/>
    <dbReference type="OMA" id="ECKVAFN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010570-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12564" checksum="436DB95FD75B4C6E" modified="2013-10-16" version="1">MTTQGVDLKLKPHLLPNTQPIAELECKVAFNLLTKQEKLYAHYLSKASQVGALISSVQSSPEAPLLISLLRRIFTLQHPDDLRKHLLAEGVSDENITAFYVYACDKVEIDRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H312</accession>
    <name>T1H312_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H312"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010621-RA">
        <property type="protein sequence ID" value="MESCA010621-PA"/>
        <property type="gene ID" value="MESCA010621"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199951_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010621-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="11502" checksum="B699EAEC63EE8858" modified="2013-10-16" version="1">MSEVKNSDSSGVKGSSLVEGCAGVDEFHGSLSENVIDTAVAHRKNLVNAFGGSDDFSCADGGYKYMNAGTYNIHKFLNVIKGTGNIRLKKGFYSWKFGSCSTSVSSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H388</accession>
    <name>T1H388_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H388"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010707-RA">
        <property type="protein sequence ID" value="MESCA010707-PA"/>
        <property type="gene ID" value="MESCA010707"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624803_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010707-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9188" checksum="F5792B8D864A14B2" modified="2013-10-16" version="1">MAFTRWSHHKPNFPCLIRKKTLLKDNIETTTICKSTEKVQYRDFDYQLMEVIMKCFPAGLRRSAEMASEPALFLFLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3X6</accession>
    <name>T1H3X6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3X6"/>
    <dbReference type="STRING" id="36166.T1H3X6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010973-RA">
        <property type="protein sequence ID" value="MESCA010973-PA"/>
        <property type="gene ID" value="MESCA010973"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1082935_0_0_1"/>
    <dbReference type="OMA" id="HSVEMAV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001134">
        <property type="entry name" value="Zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010973-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="257" mass="29125" checksum="97DAFF0C3735F872" modified="2013-10-16" version="1">MGLDKFTIHLFSHCDVENGSQIQAPLPPAVVPPILESGNDTFGAVHSPNQETAIAETGDRASARFQCKLCSKSFNIRGSLKVHMKVVHSVEMAVADKVNNVGGESNKCPSDDPIKMWECDICHKMFTTKYFLKKHKRLHTGETPYNCEQCGKTFTFQQSYHKHLLYHSDDKPYICQNCGRAFKELSTLHNHERIHSGEKPFECETCGERCAVRCPTFLQNVIDQKLHLTSDSPTNFRKIGTHFIKICGNSICFERFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H426</accession>
    <name>T1H426_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H426"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011025-RA">
        <property type="protein sequence ID" value="MESCA011025-PA"/>
        <property type="gene ID" value="MESCA011025"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471616_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011025-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9934" checksum="EAE8614804D9B888" modified="2013-10-16" version="1">MNNNYRQLRNRKVEIVYEGRTPSSDPYSEDEEYSNPSLPRTSSPLNSETQEGGSWNSPKSAPALIGVQGVTQYLAFPLMAKAYFKLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4W8</accession>
    <name>T1H4W8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PI3K/PI4K catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4W8"/>
    <dbReference type="STRING" id="36166.T1H4W8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011339-RA">
        <property type="protein sequence ID" value="MESCA011339-PA"/>
        <property type="gene ID" value="MESCA011339"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943431_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF68">
        <property type="entry name" value="DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011339-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14688" checksum="A1A41335CA5C34E3" modified="2013-10-16" version="1">MICGSATLDLPVPELVPFRLTPQLTAILEPIGTSGLLEKSMVHVLRVLRNSKHILLACINVFVQDPIVDWFHLIKCSSGIEKKDIQAKLELRINSVQHKLEGYNPKEICISDLENSKINTNEEYLHAYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5H5</accession>
    <name>T1H5H5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5H5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011552-RA">
        <property type="protein sequence ID" value="MESCA011552-PA"/>
        <property type="gene ID" value="MESCA011552"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847395_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011552-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15446" checksum="BD2C8F073EDC2BE3" modified="2013-10-16" version="1">MPHRAWASFQVASQISSEEDLRNIPDNGGLTHLLVDTETPFLQSILDQFHDTLTHLVVGNSSFFSVDLTYASLKSLSILNCNITDDGVIEKLVENAPCLEELYLGNYKSATDKHVLIITTEAGVNRIHMKFIGKLSKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5M8</accession>
    <name>T1H5M8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5M8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012011-RA">
        <property type="protein sequence ID" value="MESCA012011-PA"/>
        <property type="gene ID" value="MESCA012011"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2678097_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="2">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012011-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8436" checksum="DC52EA58310A292A" modified="2013-10-16" version="1">MARTKQTARKSTGVYIVDPQIAFPTFVREIAQDFKTDLRFQSSAVMALQEAXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6E5</accession>
    <name>T1H6E5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6E5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012278-RA">
        <property type="protein sequence ID" value="MESCA012278-PA"/>
        <property type="gene ID" value="MESCA012278"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340512_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012278-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23888" checksum="7B4298C900D7FA26" modified="2013-10-16" version="1">DHCMALKIIKNVEKYREAAKLEINALEKISAKDPNCEVHMLDYFSFRGHMCLTFELLNINLYEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIYRNHTLFSKTKTKYFYHGKLDWEEKSSAGRYVRDHCKPLFRYLMSDSDEHYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6J2</accession>
    <name>T1H6J2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">IMP dehydrogenase</fullName>
            <ecNumber evidence="11">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="T1H6J2"/>
    <dbReference type="STRING" id="36166.T1H6J2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012325-RA">
        <property type="protein sequence ID" value="MESCA012325-PA"/>
        <property type="gene ID" value="MESCA012325"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_1"/>
    <dbReference type="OMA" id="TYDDVFM"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000424">
        <property type="entry name" value="Inosine-5'-monophosphate dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="81"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="143"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012325-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="313" mass="34068" checksum="7A7CEDEA79833CBE" modified="2013-10-16" version="1">DFLVLPGYIDFTADSVELSTPLTKKVQLKTPFVSSPMDTVTEADMAIAMALCGGIGIIHHNCTPEYQGNEVHKVKNYKHGFIRDPYCLSKDHTVGDVWELKRKNGFSGYPITENGKLGSKLLGIVTTRDVDVSLPATKLESVMTPFKDMVTAPNGVTLQEANEILERSKKGKLPLINEKNELVALIARTDIKKARNYPRASKDGNKQLLVGAAIGTRKEDRDRLKILVENGVDVIVLDSSQGNSSFQIEMVKFIKNTYPELQVIAGNVVTRGQAKNLIDAGADGLRVGMGSGSICITQEVMACGCPQATAVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6U8</accession>
    <name>T1H6U8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6U8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012430-RA">
        <property type="protein sequence ID" value="MESCA012430-PA"/>
        <property type="gene ID" value="MESCA012430"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2694944_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012430-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8056" checksum="EB78503D132F7531" modified="2013-10-16" version="1">ESISFTVDSAVPATTNALGDGYDFITVTGLSFESNIPMGLPMKGSHKCTPESQEAMYLKDSRKASETDFLWAVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H757</accession>
    <name>T1H757_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H757"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012539-RA">
        <property type="protein sequence ID" value="MESCA012539-PA"/>
        <property type="gene ID" value="MESCA012539"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929427_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012539-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6933" checksum="76567539A32ED651" modified="2025-10-08" version="2">MXXXXYNLLPKGALSLRVLQELPIIVVLMYQIYKATIQNEVAEFVPLIMTTITLQPSIAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7B9</accession>
    <name>T1H7B9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H7B9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012657-RA">
        <property type="protein sequence ID" value="MESCA012657-PA"/>
        <property type="gene ID" value="MESCA012657"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005746_6_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF637">
        <property type="entry name" value="EAG-LIKE K[+] CHANNEL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="386"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012657-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="683" mass="77739" checksum="9F48ED8252D05428" modified="2013-10-16" version="1">YGPDTKEDHKLQIEKSLQAKTELKLEVIFYKKDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNLLHSQETPFPEYKTQSLKKSRFILYHYGVFKGFWDWIILVATFYVAIIVPFNAAFAKLDRQTLVYDMIVEALFFIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAFHIHLVKLTRLLRLARLLQKMDRYSQYTAMILTLLMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWMHALAERLKVPVANITNAEAYSTALYFTFTSLTSVGFGNVSANTTAEKVFSILMMLIGALMHAVVFGNVTAIIQRMYSRRSLYESKWRDLKDFIALHQIPNELKQRIEDFFQTSWSLNHGIDIYEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALTYCDLKCVYIGGLVEVLRLYPEYQQQFANDIQHDLTFNLREGYECQDSDIGPSLPLPSISEDDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Y3</accession>
    <name>T1G9Y3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159248">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Y3"/>
    <dbReference type="STRING" id="36166.T1G9Y3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000025-RA">
        <property type="protein sequence ID" value="MESCA000025-PA"/>
        <property type="gene ID" value="MESCA000025"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_547010_0_0_1"/>
    <dbReference type="OMA" id="CNCANNS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048407">
        <property type="entry name" value="Dumpy_DPY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22963:SF39">
        <property type="entry name" value="DUMPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22963">
        <property type="entry name" value="ENDOGLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21164">
        <property type="entry name" value="Dumpy_DPY"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90148">
        <property type="entry name" value="DPY module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="61"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000025-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="437" mass="47581" checksum="5892BC76C46F35A9" modified="2013-10-16" version="1">ETTLCNPNPCGVNSECFVKGNSYSCKCIRDYIGNPFEECRPECVENSQCLANKACINSKCLDPCATSPCGIDSECYVNNHIPVCVCIKGYTGDAFKQCTGITVRENDCDGNICGPNAQCKIVNGRGICECLSGFFGDPYNIGCKPECVISSDCPKNKLCLKSKCRNTPGCVSNDDCTDNEACINNKCRDPCNCANNSQCQVKNHRAICSCEPGFEECYMEQNRPLCRCKSGYRGNPFVECKIIECLTNSDCPTNKQCSNEKCINPCIYNNKCGAKAECFVQNHKDICRCPQGYYGNPFVKCMQNTEECVIDSDCPESLACINARCVNPCHQLEPCSRPSTCAVISSKPMRTMICTCPDGYVSSGSGTCKPTKSIVEIGCISDVDCDSTKTCVAGICKNPCDSNACGSNAECRIKAINLFALVPKDMKEIQKLDVSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA22</accession>
    <name>T1GA22_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000065-RA">
        <property type="protein sequence ID" value="MESCA000065-PA"/>
        <property type="gene ID" value="MESCA000065"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725117_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000065-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8281" checksum="080428766DFD609D" modified="2013-10-16" version="1">MDFVFQMKNVFAKKDIEFQIKVISAILCAPTIAIMEFAYHRILACSIIVLQKKDLFVIFVKGNSHDPDILHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA23</accession>
    <name>T1GA23_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000066-RA">
        <property type="protein sequence ID" value="MESCA000066-PA"/>
        <property type="gene ID" value="MESCA000066"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029358_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000066-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="14220" checksum="08B0900E535ECA7C" modified="2013-10-16" version="1">MNHTCHESSINDTHERCKYLFRSLPSNSMFTLMNLRKTINLELERNMFKPLKKWIPANSSKNLGECLFFYENDDPSSNSSTKWRDVLVVERLQGPIGLKSGMIWEISRPQTWRSLTVGHGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA58</accession>
    <name>T1GA58_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GA58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000104-RA">
        <property type="protein sequence ID" value="MESCA000104-PA"/>
        <property type="gene ID" value="MESCA000104"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1099563_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46901">
        <property type="entry name" value="GH04942P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46901:SF2">
        <property type="entry name" value="GH04942P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOMON" evidence="2">
        <location>
            <begin position="116"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000104-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="253" mass="28639" checksum="216703AE459A1133" modified="2013-10-16" version="1">MPSELALLMYSLECNSNLFYCFGGGIEDKQFCLRGHSALIAEEFLPSTKNSFFDPFISEFITVASSSEPEPKSEPEPKSEPEPKSSAEPKAEPKPKAAAPKPKLNLEPYTPRHDFNPMDCTDVVIGTARGKTSRVGDYYSRDRSTPKLDEVWGGKSDLTFSSGFEEDGVTTIIFRKKLEASEPTDHSFDDQLMXXXXXXTIIFRKKLEASEPTDHSFDDQLMQIRQFYQPDEIKYHGHQMQRGVAQINFFGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAB1</accession>
    <name>T1GAB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02134296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAB1"/>
    <dbReference type="STRING" id="36166.T1GAB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000164-RA">
        <property type="protein sequence ID" value="MESCA000164-PA"/>
        <property type="gene ID" value="MESCA000164"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026811_4_0_1"/>
    <dbReference type="OMA" id="NCTEHGP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042805">
        <property type="term" value="F:actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060537">
        <property type="term" value="P:muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09404">
        <property type="entry name" value="LIM1_MLP84B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000001">
        <property type="entry name" value="Cysteine and glycine-rich protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF35">
        <property type="entry name" value="MUSCLE LIM PROTEIN MLP84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000164-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="11840" checksum="910450FD065C1402" modified="2013-10-16" version="1">MSLMRDSSESSFEWGERGSYTETPKCPCCEKSVYAAEERVAGGYKYHKTCFKCGMCNKPLDSTNCAPHEKELFCKNCHARKYGPKGYGFGGGAGCLSMDDGSHLRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAF8</accession>
    <name>T1GAF8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000214-RA">
        <property type="protein sequence ID" value="MESCA000214-PA"/>
        <property type="gene ID" value="MESCA000214"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2335994_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000214-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="10708" checksum="A840AC2AAC3F26BE" modified="2013-10-16" version="1">MPLPSTSKQHKTVMTSSSIDSNSNENTQIEDPNATAADDKFQLSGGHMNILKMCKTNTSAYSAMVDSVYEIELHNLEAGSEESDLTRMFVSDDDGDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAN6</accession>
    <name>T1GAN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAN6"/>
    <dbReference type="STRING" id="36166.T1GAN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000295-RA">
        <property type="protein sequence ID" value="MESCA000295-PA"/>
        <property type="gene ID" value="MESCA000295"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1096560_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005790">
        <property type="term" value="C:smooth endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005219">
        <property type="term" value="F:ryanodine-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014808">
        <property type="term" value="P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006941">
        <property type="term" value="P:striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399">
        <property type="entry name" value="B30.2/SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399:SF8">
        <property type="entry name" value="B30.2_SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000295-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28496" checksum="8D8F6EF72061B5C2" modified="2013-10-16" version="1">EEIEFLLACCERNHEGKIDYAAFVDIFHEPSTEIGFNLAVLLTNLSEHMPNEPRLARFLETAGSVLNYFEPFLGRIEIMGSSKRIERESKKAFFYSIVTEGDKEKLEAFSIPELIIGFLKLIFYFFYYTGYANFAVVRYFAGILLSLMRGPPPEEVEEAPPEEDRFGRRALPALPQDEAPSTMSAFGLDISKEENGQFKVAAHESPGVSPTSSVEETEGSTPEEVQIGPNGEPIIVEPHQEPVSIVDLLERQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB29</accession>
    <name>T1GB29_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">26S proteasome regulatory subunit RPN12</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD8, a base containing 6 ATPases and few additional components. Interacts with DDI2. Interacts with TASOR.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB29"/>
    <dbReference type="STRING" id="36166.T1GB29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000450-RA">
        <property type="protein sequence ID" value="MESCA000450-PA"/>
        <property type="gene ID" value="MESCA000450"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046003_2_0_1"/>
    <dbReference type="OMA" id="LESAYMH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="77"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00078986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000450-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="22198" checksum="18B32435B4EB1E7D" modified="2013-10-16" version="1">MVASVENLYKELKAEWAKKPLNLKKCGEILDQLKIALTTKVCFLPTGCVSTNKAELILARDILEVGVEYSVHTKDIPAFERYMCQLKCYYYDYRLDIKESTNMYKLLGLNLLFLLSVNRVAEFHTELELLPANIIQSNQFIRPILALEQYIMEGRYNKIFQAKSSAPSEIYNYFMDILTDTVRDEIGACLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB51</accession>
    <name>T1GB51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dedicator of cytokinesis TPR repeats region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129188">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB51"/>
    <dbReference type="STRING" id="36166.T1GB51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000472-RA">
        <property type="protein sequence ID" value="MESCA000472-PA"/>
        <property type="gene ID" value="MESCA000472"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751807_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF12">
        <property type="entry name" value="SPONGE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dedicator of cytokinesis TPR repeats region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000472-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17651" checksum="C349373A2A46614F" modified="2013-10-16" version="1">MASNNVIRKSLEEFSNPLVYKFLQHDRFDMELWCEYFKVVVCFVTQSTLQIEKYPELKKRQVLQNYGDMRVTMGFQVLSMWSQLGDKKCNFIPFMIGPILEITLVPETSLRKSTIEVFYDMIQCEQHESERDGKVDLVEKQNHKISQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB99</accession>
    <name>T1GB99_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDXDC1-like third domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB99"/>
    <dbReference type="STRING" id="36166.T1GB99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000526-RA">
        <property type="protein sequence ID" value="MESCA000526-PA"/>
        <property type="gene ID" value="MESCA000526"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393320_0_0_1"/>
    <dbReference type="OMA" id="MNEWNKK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055102">
        <property type="entry name" value="PDXDC1-like_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF1">
        <property type="entry name" value="PYRIDOXAL-DEPENDENT DECARBOXYLASE DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22937">
        <property type="entry name" value="PDXDC1-like_cen2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDXDC1-like third" evidence="4">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000526-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="21840" checksum="15AD5CDD3B433607" modified="2013-10-16" version="1">MATITHKATFNDLVEKSDVLRIVNLPDWAGMGGVQFVPEGWESILTDQAKTELNKLNIDLVEALKTTDSAFSMGEGSDGLICVRFGMVTHETDVEELLDLVVSVGKSVQENSKVLDTMSEIVKKGIEAATADLQRETEEKLWQEGLLRHVPVFGRVMNWWSPPEKESGIRGRSLNLTQGVVESTENIYKYHMQMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBF3</accession>
    <name>T1GBF3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02038221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBF3"/>
    <dbReference type="STRING" id="36166.T1GBF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000585-RA">
        <property type="protein sequence ID" value="MESCA000585-PA"/>
        <property type="gene ID" value="MESCA000585"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848076_0_0_1"/>
    <dbReference type="OMA" id="VEYNHIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000585-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="328" mass="38216" checksum="557B64824907F7EC" modified="2013-10-16" version="1">MNYVFATQEQTPGSGSILLLNIIFDKNNLIYLDLSRNLFYEIPAIVLKNKKSLRTLILRENNIKSVDTLLASEFPCLEKLDLGENKIMELPLEMPPTLKYLDVEYNHIRSISHLTLEKLNLTYLNMKSYYKDLVKNNIWSMKHLEELFQTIDSNTITLQSCENLKVLYVFFFGISQFNNFESFVRKCQSLRTLELTFYGSLRNSNEIIILNNIVLKELRMFSIFCDSMYKLNTSFLDNHKQLTDVSLSGEITDLDKDTFKSQQRVLYIGLTSTKLEELPETIFSYNSHLIKLNLQSNKLTNLSKNIFNNLKNLIILNLSKNQLTNLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBG8</accession>
    <name>T1GBG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Electron transfer flavoprotein alpha/beta-subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02106781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBG8"/>
    <dbReference type="STRING" id="36166.T1GBG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000600-RA">
        <property type="protein sequence ID" value="MESCA000600-PA"/>
        <property type="gene ID" value="MESCA000600"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1327712_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="5">
        <location>
            <begin position="24"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="22404" checksum="AA489AF1D4CDDF3A" modified="2013-10-16" version="1">MARVLVGVKRVIDYAVKIRVKPDKTGVVTEGVKHSMNPFDEIAVEEAVKMKEKKLATEVVAVSVGPAQSQETLRTALAMGVDRGIHVEISGKEFELLQPIHISKILAKLAQEEKADLGFEVTFPTLAGIGAIVGSGLFKGRESISFISLTDLLLLRWVRFISGRGLASGDAALTEINQLDYIAAALMKLLNKYLTSWSLSEIGDVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBK2</accession>
    <name>T1GBK2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Oligomer composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02062140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBK2"/>
    <dbReference type="STRING" id="36166.T1GBK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000636-RA">
        <property type="protein sequence ID" value="MESCA000636-PA"/>
        <property type="gene ID" value="MESCA000636"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_2_1_1"/>
    <dbReference type="OMA" id="IFIEMSP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098957">
        <property type="term" value="P:anterograde axonal transport of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007292">
        <property type="term" value="P:female gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000067">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF68">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="204"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="294"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000636-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="383" mass="43487" checksum="004B22F1A747418A" modified="2013-10-16" version="1">MEMNLEFHIKVSYYEIYMDKIRDLLDVTKVNLSVHEDKNRVPYVKGATERFVSSPEEVFEVIEEGKSNHMNEHSSRSHSVFLINVKQENLENQKKLSGKLYLVDLAGSEKVSKTGAEGTVLDEAKNINKSLSALGNVISALADGNKSHIPYRDSKLTRILQESLGGNARTTIVICCSPASFNEAETKSTLEFGRRAKTVKNVVCVNEELTAEEWKRRYEREKEKNARLKGKVEKLEAELARWRAGETVQAEEQVNLQDVMEASTPNLEVEETVKAATPSANLTAMVGSITSDERTRLEAERERLYQQLDEKDEEINQQSQYVESLKEQMMDQEELIANARAEYEALQSEMSRIQQENSNAKEEVKEVLQALEELAVNYDAKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBQ5</accession>
    <name>T1GBQ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBQ5"/>
    <dbReference type="STRING" id="36166.T1GBQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000692-RA">
        <property type="protein sequence ID" value="MESCA000692-PA"/>
        <property type="gene ID" value="MESCA000692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="OMA" id="PARRWNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000261">
        <property type="entry name" value="cyclin-dependent kinase-like 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="328" mass="37126" checksum="D70BAD9413DE0C62" modified="2013-10-16" version="1">SLFILCVHQIYTYNNIQHDSNLQQQNLKHPNIVSLLEVFRRKRRLHLVFEFCELTVLHELERHPLGCPENLTKQITYQTLHGVAYCHKQGCLHRDIKPENILLTAQGQVKLCDFGFARMLSAGENYTDYVATRWYRAPELLVGDTSYSTPVDVWAIGCVFAELVRGEALWPGRSDVDQLFLIRKTLGDLLPRHIQIFGQNEYFKGITLPVPPTLEPLEDKMPAKSLQNPLTIDFLKKCLDKDPMKRWSCDKLIRHSYFDDYIAKQKENNDNVSNTLLMDSSKTVGIGLNGGGGILGLGNGAGGGGGSRDKSKVSQFLSFFQTKVKQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD85</accession>
    <name>T1GD85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001266-RA">
        <property type="protein sequence ID" value="MESCA001266-PA"/>
        <property type="gene ID" value="MESCA001266"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624843_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001266-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8702" checksum="2CE8F9F303127685" modified="2013-10-16" version="1">MAWTSPPIKTSDAKEKGRLRTNCNGVRLHHGTCVLCVCRISFVSLMRSVHEPSSFMRSDGIVLGLVMIFLSLFVISTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD94</accession>
    <name>T1GD94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02038362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GD94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001276-RA">
        <property type="protein sequence ID" value="MESCA001276-PA"/>
        <property type="gene ID" value="MESCA001276"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197447_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048362">
        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF15">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG helical" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="5">
        <location>
            <begin position="99"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001276-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="232" mass="26200" checksum="56DC93BD2EBE3443" modified="2013-10-16" version="1">MPELIQAPIPLLKESKQHSISLSQEQISCLLANAFLCTFPETLEHYPDINFTRLFGSPGYEVVVEKLRCILHYFRSVCDNSPLGVLTFERRYIPTNELPNWDQCDLLLGDTLLRMTSEGTIEDQGTGLLQVDFADKYLGGIVLGVPCAQEEVRFLINPELIVGKLFTERLLPNPXXXXXXXXXXXXXXXXXXXXPPPPPPFRLFGSPGYEVVVEKLRCILHYFRSVCDNPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDV8</accession>
    <name>T1GDV8_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001509-RA">
        <property type="protein sequence ID" value="MESCA001509-PA"/>
        <property type="gene ID" value="MESCA001509"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796871_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001509-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7743" checksum="D77FF0277F2D1180" modified="2013-10-16" version="1">MKRGYLFEKFFDYSFNPDKVIARSTLGRSNSTISPLSITITLSESIIVFNLWAIVNIVHSLNSVLMVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDX0</accession>
    <name>T1GDX0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDX0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001521-RA">
        <property type="protein sequence ID" value="MESCA001521-PA"/>
        <property type="gene ID" value="MESCA001521"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1086995_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001521-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="256" mass="29472" checksum="FBF1DE1C2FE4C661" modified="2013-10-16" version="1">MEDEMLKKKSLNTTKVYNAAWEKLPIFKGWLTNGNKGALYGRCIACDRYLRAQRNDIYKHSQSMLHKKNMTRAGMKAAKGPYPFKKVGSPRVHKPSTQSKIAEAEANPENEDFSDSGTEYLVESLNENELYNTETLEENEEQEAFDAFEEVEVVDPNQSKSSTKIPQIFSEEECDIFGAFITNEMKALDPEKRKIFKKKTQTNLPDENFKIQLPETIDISLMAVSSDEEDSDDEFEMKLDRNKIEDDEEPFFGFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDZ6</accession>
    <name>T1GDZ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDZ6"/>
    <dbReference type="STRING" id="36166.T1GDZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001551-RA">
        <property type="protein sequence ID" value="MESCA001551-PA"/>
        <property type="gene ID" value="MESCA001551"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707611_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001551-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8088" checksum="EAD972813A44EF1F" modified="2013-10-16" version="1">MFVTGLSFLPVKNSNQGPTISSNSELAVLSISIDNKICIHSVQNRHTIPVWLSILLIIFVLFLTFVILSYIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE07</accession>
    <name>T1GE07_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02106968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE07"/>
    <dbReference type="STRING" id="36166.T1GE07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001563-RA">
        <property type="protein sequence ID" value="MESCA001563-PA"/>
        <property type="gene ID" value="MESCA001563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022406_1_0_1"/>
    <dbReference type="OMA" id="NALWINS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000584">
        <property type="entry name" value="Dynein intermediate chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="225"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="413" mass="47674" checksum="D104592B898EBED6" modified="2013-10-16" version="1">MNTIEYRYKKDRKCFGQQVQFDDKEQVLFSEGSNHELFKEYIYQNPVDRETSHTRQMAFSIANTERATYKHCGVTHNEGGWPKDINMHDNEQTVRYKRKIEKDESYITQVMNLIKPVEHCIFQTTQIKVPITHLSWSPDGGLKLAASHCDMKFQGNKDGQGPCSYIWEIENPNEPFLSLEPKIPCVCLEYNQKDPTSLVSGMYNGQVAAWDTRHGKFPVMMSERESGHREPVNSVLWNNSKSGTEFFSGGSDGQVLWWDTRKLSEPFDKLLMDPVRTDDQELSRSYGVSILEYETTIPTRFMSGTEMGMLFSCNRKGKTPIEKIQIKMNCHLGPIHAITRNPAFVKNFLTIGDWTARIWSEDCRESSIIWTKSNEAMLTGGAWSFTRVSLFFTTRMDGVMDCWDLLQQQNEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE22</accession>
    <name>T1GE22_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE22"/>
    <dbReference type="STRING" id="36166.T1GE22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001579-RA">
        <property type="protein sequence ID" value="MESCA001579-PA"/>
        <property type="gene ID" value="MESCA001579"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149652_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="97"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="171"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001579-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="26754" checksum="01836EBD79389B48" modified="2013-10-16" version="1">IESKKELKSVAKISQSKSSHVFDEVPSVVQVPMASFEQDLDDDFTYHPTPSSNPTTTEREWTCKKCTLVNSSTSSVCIVCGGSKLKSISSVEDMTLRKGEFWTCSQCTLKNTLAQNTCSACKHVRETPIISGQLSNFRPYTEQNNIVAPTNSSHKTSQNNNRQQQLNLTNTTSTILSPSSPGLQPPSNRTSRSPSPRHERYSGAIPKSPLMNLENFIRFMDHLHSSLCSVPIYQGSNPGNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE29</accession>
    <name>T1GE29_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Codanin-1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE29"/>
    <dbReference type="STRING" id="36166.T1GE29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001587-RA">
        <property type="protein sequence ID" value="MESCA001587-PA"/>
        <property type="gene ID" value="MESCA001587"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007271_0_0_1"/>
    <dbReference type="OMA" id="CVVKDAQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040031">
        <property type="entry name" value="Codanin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028171">
        <property type="entry name" value="Codanin-1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28678">
        <property type="entry name" value="CODANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28678:SF1">
        <property type="entry name" value="CODANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15296">
        <property type="entry name" value="Codanin-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Codanin-1 C-terminal" evidence="2">
        <location>
            <begin position="716"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001587-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1034" mass="118509" checksum="279DE31DC6A2B341" modified="2013-10-16" version="1">MTPEEILQKLCRESNGNFQQFFLNQFSSKCSVEEFGVFFLNFLRQQTEIFLKGGGGSTATPLFTPLKPTTDENQAKDLSFRDCSSSSLTLDSKPISTKKKIRTSLFEQSDFPEQNKSMEQHSLNSTQTSANNSRSIREFSLEDFMNTSTPSNRVKSKKTVQKKRVVPITLSKSVNDSDSDAFTSPAFRNDNNILKINDTSEIMDRKKMKNEMTEISKQIEEIQAPAVFSLRTSVAAVAAAESPVKSKPVEICLDSIDDGTKVVLKRLAEIYCILMDLHLTANILTEISFILNILDADGIMTSPLNPDPETDEIDRKLINMGFSEVFLVFKDFTNCVFFALEVLNRRRGTLAVLDLYSLKVIIENNRISTAHPQLTAYLQTVYSKKKQRDAFYAILKIWEQKHLNPLWNFKEGLGTKIQNTIFHVSEHPINMAHLAQLFLSQLLVSANEPLPQELSDVDQIKLSKLTQRLVTPGSFSTNYQFPGNQMFFKEFLQTTGSHIFMEQIKLVLIANLLELNDSTYDILNLSSNPQAGNEGSTEYVVRTEVISNLRILSKFLGLVVSIPYTYEGNKNSIMDQKQLELKNNVTSHIDFVKILKKALVEKKLLITIPWMIQYLVMLDETTLKHNHYKQLFSLLFEIYWKIDILKLRQTSNFIILSCLGWLFEQLNLVGDYYEHRQTKSPKTDLSLLVDFQEVPIFLPSNSNLISEIEDNVLVGSLLESILNASCPFLADFRVAIMPKGVAKTLSRTGRYRHITTKIMTEQQSSPSVQQQSVQDNKTKLVEAFLYSQSLSVRRTIEFVIERTSSAVIKDFQVKFLLPSKTQALENIQYICDTDFPSIYKNIKEIFTKSFNTILEQWNVETPKMIEDRASQALDALLPIETQSVVKSTCLLLVKEKCILKTSEWRKNNLKDLSFYSTDLQADAQKFQKQQQTKNNQHTNLIIRQENCNLSESLDELQYWLHNTSYSYKFVNTNDLLVFLKKYKSTFHENTLPYFFFRVTGLMTINLIQNLISYFPNVLTMEVIGEFLESGNMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF11</accession>
    <name>T1GF11_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02055125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055127">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GF11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001939-RA">
        <property type="protein sequence ID" value="MESCA001939-PA"/>
        <property type="gene ID" value="MESCA001939"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1429533_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001939-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21613" checksum="D07285CAF27FE82D" modified="2013-10-16" version="1">MEMAFTEIHKDILPNFDIFQVNGDFVDCTLVAEGKSLDAHRVILSACSIYFKEILLHNAKLKQHPIIILPNDXFINIENVEVLKPPEPEPSKNIVRYTILKKNLDKSTNVPKNSTVDISHSKNTSSTSSPSPSVSNTGNKFICDCGKSYKYNFNLNRHKNFECKIQKKAEVKLKNPKNSQKFSTVNYTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFA3</accession>
    <name>T1GFA3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02038543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFA3"/>
    <dbReference type="STRING" id="36166.T1GFA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002039-RA">
        <property type="protein sequence ID" value="MESCA002039-PA"/>
        <property type="gene ID" value="MESCA002039"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197448_0_0_1"/>
    <dbReference type="OMA" id="NERIFPC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002039-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="232" mass="26429" checksum="C44B35F586FA8315" modified="2013-10-16" version="1">ILLNLRKPSSASEKRCPHCKDSFPNILCLRKHVDEMHSNEKPSCSFCAQTFTLAQDLSRHVRDRICQKETTTSTSIELFDSSSATAAAATVSVASTSELTITQDIWCCDVCAFTTLLEAEYLFHKVLHTVSTITNQTKLTCPICNKVFRKHSLRYHLRQHTNEKIYVCELCHMSFSRKQALKQHIRNMHGSNSGSMDVGMVTLQEEKTKETKVIRKEKCYKCEECGKEFKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFC9</accession>
    <name>T1GFC9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFC9"/>
    <dbReference type="STRING" id="36166.T1GFC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002068-RA">
        <property type="protein sequence ID" value="MESCA002068-PA"/>
        <property type="gene ID" value="MESCA002068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203570_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12242" checksum="6E0915FB8A74342F" modified="2013-10-16" version="1">MRNTELKTCDLSSNVIKKIPPKFALKFNFITDLNLSHNQMSKLPSELADIASLENLDISFNSFIALPQVCFNIPKLRILKANNNAIIEIDTEEMVASDKLELVDLRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFJ3</accession>
    <name>T1GFJ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFJ3"/>
    <dbReference type="STRING" id="36166.T1GFJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002135-RA">
        <property type="protein sequence ID" value="MESCA002135-PA"/>
        <property type="gene ID" value="MESCA002135"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_1"/>
    <dbReference type="OMA" id="DWIESST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF147">
        <property type="entry name" value="EG:BACR7A4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002135-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="17778" checksum="088DDDAB1A4B7241" modified="2013-10-16" version="1">KVIIHPKYDPNLAYHDIAILKLETPTFFHPACIWTSPTSPTSNLVALGYGHKEFVGQNSNILQKVNITLFENIECQFFYKGQLNTPEGIIDSQICAGDYFATKDTCQGDSGGPLLAIIEKGRMKIPQILGITSFGSVCAGGTPGVYTRVSEYLDWIESSTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFX6</accession>
    <name>T1GFX6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02002367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002278-RA">
        <property type="protein sequence ID" value="MESCA002278-PA"/>
        <property type="gene ID" value="MESCA002278"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1322250_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002278-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="22885" checksum="14F54D86F2501B0F" modified="2013-10-16" version="1">MALIPLLFKFGVISAMVTALLFLMLKAIFLLKALLVLNLGGLLAKLITLKSPHQHQWQGWTAPTAPVESWSRTSEPPSTPSKEIHVHIHNSGQSVDTSSWQNEPYGAYSSYSTSQAAAPSAPVPIPVWRQQYSSYPVTSKHAEIRLVSKPIGAKLLTLSTLETGTDLQKSELPRFSSPLICKSFQHSLQLLLYVSRTAPQMEHLNTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFZ6</accession>
    <name>T1GFZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02065798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFZ6"/>
    <dbReference type="STRING" id="36166.T1GFZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002299-RA">
        <property type="protein sequence ID" value="MESCA002299-PA"/>
        <property type="gene ID" value="MESCA002299"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827887_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002299-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39021" checksum="3733167D11CEFECF" modified="2013-10-16" version="1">KHPRSKTSRLIRATETTNSCILPTDQGLQLWRKFETPIYKKLRRAQNFMESVAIDLISEKISYYREDRQQQFSAGYMKRSSLIDEYLKNPNLDLSDIVGVGNDLLLAGVDTTSYTTSFVLYYLGRYPEVQKKVFEEAKKFMKGKHDQIEGDALRTDIPYTRAFFSEPGQALRMCGELLSRPFMDVTIMANTYPALIDTSSLQTKISSNVSSMVAALTLQQVQDGNIQVQFQIGSKFFPTLCKVQSLEEGIDIQLGMDVLYQQHFRLRINGTTIYGHNRWTTQHPDDVQFAYNLPLGKHLRQKLIKLGYELQDPYSRRNLQDDWKQRRTYFSRFRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG97</accession>
    <name>T1GG97_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG97"/>
    <dbReference type="STRING" id="36166.T1GG97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002410-RA">
        <property type="protein sequence ID" value="MESCA002410-PA"/>
        <property type="gene ID" value="MESCA002410"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1549391_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002410-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="18992" checksum="F94F639980835C90" modified="2013-10-16" version="1">MSIKNNAKIDNVNASLVVNFKNTTQKTSPVTSPASPVDKNYHQNREIFNKLSPVTSTAPNNFEFLKTYNHQKTPSDVEQINRNLLVDDNNQVCVVAKMQKSVTITVTPQRSNSLDYLNFEEKRQLIASSLSLSDLIHCGPAAAAAAAKEVAAASTYCGGRKVKGKLEKLGFMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGA6</accession>
    <name>T1GGA6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGA6"/>
    <dbReference type="STRING" id="36166.T1GGA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002420-RA">
        <property type="protein sequence ID" value="MESCA002420-PA"/>
        <property type="gene ID" value="MESCA002420"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1241371_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002420-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="24700" checksum="3E1EF7ECF0175325" modified="2013-10-16" version="1">MFVGPILQFLEKAQSIVSVEQSPQPQQKVNYALRQSPWASPQQISVIVQESQRLIKSKLSGLQRSMQLYLSNRDTEFIIFKPIRNNIIGAFSKLEQILTSNGYTNDDMIMTSCPSAEQVSILLSSASILATENARKISTSSIESVPAYTRKISQTEKKVSFDSPAITEEQLVVEEGDSKVLEESSSQDAILEVKEESSQVTNEADEPKVLKDEVDNNVGTPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGD1</accession>
    <name>T1GGD1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGD1"/>
    <dbReference type="STRING" id="36166.T1GGD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002447-RA">
        <property type="protein sequence ID" value="MESCA002447-PA"/>
        <property type="gene ID" value="MESCA002447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_0_2_1"/>
    <dbReference type="OMA" id="KEWVESC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="26220" checksum="81BD60AA31B335BF" modified="2013-10-16" version="1">LGLIPLKADPRLFDYVVENNSDEGNENTTLEFELKAKCTRRKDVKDSSNFDHIFKNHKIHSGQIKWKPKGKQSTLYKEEDVGVIHSDILISQMRPGHELDLKLFAVKGIGKDHAKFSPVATAFYRLLPEITLNKEFYGKDAFLLQKCFSPGVIGIDDNDCAYVKDARYDTCSRNVYRYPHLAEGVTLSRIRDHFIFNIESVGALSPQDIFIESVKVLKKKCQVLLEDLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGL1</accession>
    <name>T1GGL1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002533-RA">
        <property type="protein sequence ID" value="MESCA002533-PA"/>
        <property type="gene ID" value="MESCA002533"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545199_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002533-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9465" checksum="306DA8B19611B224" modified="2013-10-16" version="1">MDGSLYTFGSPPNTTVVNGIDISWIIIALVMIKAIISSVKTSYFLAIFSYDHSFNKIDDFTRNYQETFREGQHSMFYALKIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHF3</accession>
    <name>T1GHF3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATPase family AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHF3"/>
    <dbReference type="STRING" id="36166.T1GHF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002850-RA">
        <property type="protein sequence ID" value="MESCA002850-PA"/>
        <property type="gene ID" value="MESCA002850"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309191_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021911">
        <property type="entry name" value="ATAD3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12037">
        <property type="entry name" value="ATAD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase family AAA" evidence="2">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002850-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11958" checksum="1A7232EF30EBEE35" modified="2013-10-16" version="1">MQSINNKNLYADSSALERAAEAAKALERSRIPIQAKEYEAHIEQAKVEQKRIDHEERRKTLIEETKHQQQRAQYQDQLARKRYEEQIVQQQRAQEENLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI67</accession>
    <name>T1GI67_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI67"/>
    <dbReference type="STRING" id="36166.T1GI67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003135-RA">
        <property type="protein sequence ID" value="MESCA003135-PA"/>
        <property type="gene ID" value="MESCA003135"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_498394_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003135-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="547" mass="62147" checksum="1BF7FC6FECDD07CC" modified="2013-10-16" version="1">MVTTPESDLNSNTTTSKFSIGGIVGVVTNSINSSQDYSHDNSDYQWFLDFGYRENLAHQSVLSSLCDSYAGIKELPYYDDIANHLDANLAEVDIESFRAEDMNSLLSKIPNICKSGNFNTELDNSIYKTELLFSPIKESHISIDSLDLESYPDNGDIILTCKANKSNYTIAFEGSALYSDESFYVDQNENSNFKHSNKLNSQNKIWKTSDSNVIQIDNSFTTWSKIKDTSINHISYPSKNNHLSTCLLRKCFSLPNINTNMDTTESNVHERKNESDLDKIPIKRSIFKSNNNIKFENESESTPDVSQKPAFNLVKLFIQQKSCSTDTCMDVSSGCWPSNDGSSNLYVDKESIERKNMIDSGKSSNLSRNADVDNNFPAEESFQLDSLDIYDKNKASKELLDNNSLKYLSKANIRNNHFCNVINNNFCNNVNFPSNNNQNIIMNTQTKSMQTSNYSFPSLNFVPPSFLSKLNMFGEEQKAPVFVVYPNYTLPDLNFIKQAPSNSEIFLTPFKLDQIQNHYKLQKQISNEDVFIKNLRPSQIKDWKSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI81</accession>
    <name>T1GI81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-Q</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cyclin-related protein FAM58A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin-like FAM58 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02075158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI81"/>
    <dbReference type="STRING" id="36166.T1GI81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003150-RA">
        <property type="protein sequence ID" value="MESCA003150-PA"/>
        <property type="gene ID" value="MESCA003150"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022000_2_1_1"/>
    <dbReference type="OMA" id="LKHITIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20534">
        <property type="entry name" value="CYCLIN_CCNM_CCNQ_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20535">
        <property type="entry name" value="CYCLIN_CCNM_CCNQ_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000122">
        <property type="entry name" value="Cyclin-related protein FAM58A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048055">
        <property type="entry name" value="Cyclin-Q_first_cyclin_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048053">
        <property type="entry name" value="Cyclin-Q_second_cyclin_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="66"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003150-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="256" mass="29390" checksum="C0140C6911D65F65" modified="2013-10-16" version="1">MSIITHDRKKAFRPPIFRAGGPDKNGYNLGKKANFEQVFDCQNIPATSSRLRTNYRKQSGEGLVSRYLFECSIKLSFKPLTSATAAILFHKFFKEVKATDYDEYLIAASCLYLAGKIKDENVRIRDVINVCHNTLHRDSMPLDLGDEYWSIRDAIVQAELAITRMLKYDLNFELPHKYLLYYLKSLKDWLGNEVWESIPIAKTSAGFLQDFHHSPDILRFNPNEVAVCALSLALQTYGVQIPLTEEGDDAQLWYHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIP8</accession>
    <name>T1GIP8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003332-RA">
        <property type="protein sequence ID" value="MESCA003332-PA"/>
        <property type="gene ID" value="MESCA003332"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778754_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003332-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7846" checksum="4E96124160383F8C" modified="2013-10-16" version="1">MDTVFASISSYETSRTFLSTVLIKLGKNIVFMSNQSRRFSTAIAVESQDCHYTVLRLDLVSFTLLIHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIS8</accession>
    <name>T1GIS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PDCD4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIS8"/>
    <dbReference type="STRING" id="36166.T1GIS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003363-RA">
        <property type="protein sequence ID" value="MESCA003363-PA"/>
        <property type="gene ID" value="MESCA003363"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025354_0_0_1"/>
    <dbReference type="OMA" id="ACCALDF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000009">
        <property type="entry name" value="programmed cell death protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039778">
        <property type="entry name" value="PDCD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12626">
        <property type="entry name" value="PROGRAMMED CELL DEATH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12626:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MI" evidence="6">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="6">
        <location>
            <begin position="161"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003363-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="237" mass="26740" checksum="B703895E904CD9D4" modified="2013-10-16" version="1">MAEPIILEYYEHGDTHEVAVLLDEILTGQLRPYVTAVAIEISMDHKDSHREMTSVLVSDLYGRVVTSKDIVKGFDILLENLPDLQLDTPEAPTILGNYLARAVADDCIPPKYVTKPDNLETLNEYALAAIKRADTLLHLKQGWAHLDNVWGMGGPLRPVKFITKQMTLLLQEYLSSRDIQEAHRCLRALEVPHYHHELVYEAIVMTLESLSQTTEEAMCELLKSLENTCMISPAMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ03</accession>
    <name>T1GJ03_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF1907 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02065881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ03"/>
    <dbReference type="STRING" id="36166.T1GJ03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003441-RA">
        <property type="protein sequence ID" value="MESCA003441-PA"/>
        <property type="gene ID" value="MESCA003441"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055541_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015021">
        <property type="entry name" value="C11orf54_DUF1907"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13204:SF1">
        <property type="entry name" value="ESTER HYDROLASE C11ORF54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13204">
        <property type="entry name" value="PTD012 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08925">
        <property type="entry name" value="DUF1907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01168">
        <property type="entry name" value="DUF1907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DUF1907" evidence="7">
        <location>
            <begin position="28"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003441-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="21944" checksum="6E66E8286A3A64E4" modified="2013-10-16" version="1">MRSPVKFENIIFEEKSLHVPELDEIVQVLQSALSRNFQEVTVEIVDCPNLTAEPYSLVCEGLNGSLTLMELGGFATLLPLEDKGKVYDIVETSRKIMKGKDLAIIGTGAGPVSLEKSNCEIILNMNIPLKGNLENKSIAIRINEDDEISLDPINDQQIFSYLVNIFLCEGKSGKVKFEMSAPLNAVGTLFSIPNIENKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ36</accession>
    <name>T1GJ36_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ36"/>
    <dbReference type="STRING" id="36166.T1GJ36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003477-RA">
        <property type="protein sequence ID" value="MESCA003477-PA"/>
        <property type="gene ID" value="MESCA003477"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084320_0_0_1"/>
    <dbReference type="OMA" id="NISIAMV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000067">
        <property type="entry name" value="sialin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF455">
        <property type="entry name" value="GH23975P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="9"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003477-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="264" mass="29282" checksum="F790F5E67CFA7765" modified="2013-10-16" version="1">MFWKKRRYVVVFMAFLGFFNVYSLRVNLSVAIVAMTEKREDAEGNIVQDFEWDNQQKGLVLSSFFYGYILTQFIGGYIGGKIGGNIVFGAGIGTTAILTLLTPLAAKHSLGMLLAVRIIEGVFEGVTFPCIHAVWSRWAPPLERSRMASIAFAGNYAGTVIAMPSSGLLAQAYGWESVFYVFGAIGVVWFTLWLLIVKEGPEKDKYISKDELNYIQSQLGTTGRTEVKHPWKAIFTSTAFYAIAASHFSENGDSILCLLNSQHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK07</accession>
    <name>T1GK07_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK07"/>
    <dbReference type="STRING" id="36166.T1GK07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003817-RA">
        <property type="protein sequence ID" value="MESCA003817-PA"/>
        <property type="gene ID" value="MESCA003817"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1951244_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003817-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="13391" checksum="AD485E58AEDF9804" modified="2013-10-16" version="1">MVNVLAILYSNHLVMNNYQAAAAAAAQGFGPPASPHATNAARQAGFPSANSPGPTIDMYSSSGAADSVGYVQAASPQPSGFPAIAVSRAPLNYSPHKSCQTFPTISAKTHKHQRIVVDVVSFREIDPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK40</accession>
    <name>T1GK40_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK40"/>
    <dbReference type="STRING" id="36166.T1GK40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003855-RA">
        <property type="protein sequence ID" value="MESCA003855-PA"/>
        <property type="gene ID" value="MESCA003855"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888144_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003855-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="14888" checksum="CE2729ECE36C2263" modified="2013-10-16" version="1">MGNIRTPLSLGGRQRNVIAFLVTSSGRIGPSGGLGTSKTVTLAREDGGQYRLQLKNPSGFDTATINVKVLDRPKPVRILELTNLLEILLHYIGHHQKTMEAHLLLTMSSKRRNHVLILGLRLAATAQFHLFVLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKA4</accession>
    <name>T1GKA4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Endoplasmic reticulum membrane</location>
            <topology evidence="2 11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the OST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02150319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKA4"/>
    <dbReference type="STRING" id="36166.T1GKA4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003923-RA">
        <property type="protein sequence ID" value="MESCA003923-PA"/>
        <property type="gene ID" value="MESCA003923"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098495_0_0_1"/>
    <dbReference type="OMA" id="LECHYEY"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003923-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="26267" checksum="60D6DDC8B83F9370" modified="2013-10-16" version="1">MIHSGAELKGSFSRYDFQKDTRSGQASVKNYKTILPASATGVYYRDTNGNISTSNMKILKDAVELDLRPRFPLFGGWKTHYTIGYNVPSFEYLFNNENNYLLKMRVIDHIFDDMVVADATIKIILPEGSTNIKLISPYPINRHADSLHYTYLDTFGRPVIEFSKKNLVENHIHDFNLKYNFAKVTMLQEPLLVIGFFFILFVVVIICIRLDFSIYKDKESGHHQHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKU1</accession>
    <name>T1GKU1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKU1"/>
    <dbReference type="STRING" id="36166.T1GKU1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004121-RA">
        <property type="protein sequence ID" value="MESCA004121-PA"/>
        <property type="gene ID" value="MESCA004121"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444389_0_0_1"/>
    <dbReference type="OMA" id="EEMSETC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22829">
        <property type="entry name" value="Gal_Rha_Lectin_EVA1_EVA1C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUEL-type lectin" evidence="1">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004121-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="21415" checksum="5C610B42FC36A728" modified="2013-10-16" version="1">MMRIEFRSRVACENDKLKLECNPYSRIAIYGAIFGRSEEENVAICSKNPDGNRNEPKHPSCLVSYATETVMQICHGRRRCELIADVGTFGKPCSDNISMYLKVVYTCIPRKVLKDRFETIPEPDEPQQSELDFDDDDFYDENQVYNIPEGIPQVPKGPPVLPIGFEFGDIIHHTSTLMPPLSQNDNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKU8</accession>
    <name>T1GKU8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit C N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKU8"/>
    <dbReference type="STRING" id="36166.T1GKU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004129-RA">
        <property type="protein sequence ID" value="MESCA004129-PA"/>
        <property type="gene ID" value="MESCA004129"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004304_0_0_1"/>
    <dbReference type="OMA" id="DHVHTPA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit C N-terminal" evidence="5">
        <location>
            <begin position="33"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004129-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="585" mass="67972" checksum="857A48A2599DFE7B" modified="2013-10-16" version="1">MSRFFAGGSDSESDSSEEEVQQPTFGKIATTYAFSDEEEDVKRVVRSAKEKRYEELSNIIKNIRNSKKIKDMSSILTSFEDLTRAYTKALRVITKEENGITPRFYIRCLAELEDFINEVWEDRDGRKNLSKNNLKSLGTLRQKVRKYIKDFEADMKKFREAPDAESEEEVQKVESESESEDEDVKKPAPAVKAVSFKKEEKAPKPAKADDDSDSSVDWGSSDDSETESSEDENVYTSIREKFLKKQPTDKEDKYEKDDKKKEKKVKEKTHKKKEDDDEEGWETDAEINSELVLSKLLEIMSARGKNRTDRKISIELLYELKHIAEQNKMNIAIIAKILFSIITAIFDYNPKISEPMKIEFWDKLLKAQQDLLQILLSQKETITLAYGITEDQEQLETAPYFIRGCALAIVEKLDDEFIKLLKECDPHSNDYVERLKDEVNVTKIIDSVTEYIERIGNGNEICRAYLRKIEHLYYKFDPTVLNKKNGKLPASTKTSVDEMQKLCNYIYTNDETDRIRTRAILVHIYHHALHDNWFQARDLVLMSHLQDNIDNADPPTKILYNRMMANLGLCAFRQGNIKDAHRWVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLF5</accession>
    <name>T1GLF5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLF5"/>
    <dbReference type="STRING" id="36166.T1GLF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004356-RA">
        <property type="protein sequence ID" value="MESCA004356-PA"/>
        <property type="gene ID" value="MESCA004356"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1620933_0_0_1"/>
    <dbReference type="OMA" id="ASQWHLE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034722">
        <property type="term" value="F:gamma-glutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046900">
        <property type="term" value="P:tetrahydrofolylpolyglutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF0">
        <property type="entry name" value="FOLATE GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR615527-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004356-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="19100" checksum="F3E3D2F171D45A7E" modified="2013-10-16" version="1">MTILVPGGRVYFEDKYNNDSIFPNKCVPSFQKIYKIALDMNKNRKQYFPIFGICLGFQLLLCSSNDNIQRIEGCNNEENSVPFHILESGKLFENLDMIGNPNVRYNQIYRILEETLKNSDWNILSTAEDRDGNKFVAAVEHKDYPFFGVQFHPEKSMEIQEDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLL0</accession>
    <name>T1GLL0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fascin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fascin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02192025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192026">
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        <property type="molecule type" value="Genomic_DNA"/>
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        </source>
    </evidence>
    <sequence length="1707" mass="185953" checksum="3F72AA73BBF1B7FC" modified="2013-10-16" version="1">MIHLIQRWAMSGESVEPAAQKNQVPRKHGMQSLGKVPSTRRPPANLPSLKAEWFNVNNNNNNTSSTLTSSKQDNLINSSSSAQGTLLNHHHHQNHSSSSSSAAAASSSIQQTLTNSSSSSITWSSVTTGHDVMGAGNNNAVPPHYQSPQFQHEFPSLDGSVSGNKGSSNQQPNREGNQQQQQHHHHQHQQQHHSSSSSHSHHSSNHQQNFRDGNTGNGNEVSLRPQTDAASWHQRNQHMQKEQQMHEQQISQQTAGGNTKFTSNDSSINNQSGEGSGRMGGGQSAGQGHQQHHHNNAAAVVPPPLRALMPSFMMRGTGDSGQGSGSRSLTPPTGQQQTNGSNYQQRGNYQQNRSASPNFLRGQGGGGGGKRPSPLEEELERMNALAKSDGWAMPDDIDYNKQLALSDDESPENTPTVERDRPPQFPKISNKKDEKQNGHRPSTNVDQAALERAKLRKEEEERREQERLVAARKKLLELEMKMGASSTQSQTLSGPNSATAQQTTTNIENDDEGSDSASNRRQFGSAAGNRYDRERDFRPAQANKANFSSHFQSNLPPRFQRQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNYKQQQQQQQQQRRNAGFGRNRSDNSENDEDFTRYNRRSGGDSDHQGRGITRSISDSSSNRKISCSSNDEPQRSDSRDHLPSGSWADESDMHVGRRHRDESFSSSHSKTDEMEQGHVKILQRPPQSSQAGGQSQNKSLSESSTKNEKILEEEESDSKSFAPTQILRRTESPKPMADNAHPDESNKTDIKDLEKENIKDEKLKDQGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDSRGNRHYNDWSESENSEMGDDDYAGRRNDRSPKNPKKDHGSSRNVGSGHKEGFAPRGEPSRRGRGGGGNLSVSSSSFRRGGSNSGSNSMSTSRRNDGYNSKSGFPSSSNDDIKDKSVDDAKKRELEKKDDKKGTTPPKSSSSSSVNSSYQHTRKESTSSSKNEDLGKRDDKKDNIPQRSASSGQIKTSPGSTSTSAPTAVSGSSGNSAKKDDRKQSITKPPPGYNTNKSTVSPNLNPLHKKVISNSSANPAKSPGNSGQQQQQQQLPPQQWVSSSETSVTSVTSTSSSQILDGSSAPVNTIIFENTNYKTSVTTLEGAKQDPATVIDTTEALQASLTQISLNAADVAAAAEYDKEMKMGAFNCFDAEFPTRMTSSGSAVNDDKSTSLPRSSSNSSSVVSSASDLNLKIASVKKVWDTTTLSPLMEQHNANEAAEQLQQQAQQMHHHQQIPQQQLQHLASHIHAYTFEDASNAGVVVAQDSAVDVVKQAVDVNKVKSSVQQQQQQQLHHPNLGLSPPPTIAPSQSGMYPYIEPRSQFSQFPAHYGTTGTGAPYNAYMQTPQMQTAPQQAQNADFYANIRSAYPQYRMSGTVQSFNQNQQINNPNAVLISSSSNSLISASSKPPSNQQLGTIGTKNNVDAAIASVNYGQQYMNLYQQQGPPPHGGHHSQIPSSNYYSNSAGAYFSAAANSGAGTQSYGMFGGHAGSTGGSHSTNGPPPGPPQQIQNFSSQFQGNAYNTLLAPAALNQFRGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM50</accession>
    <name>T1GM50_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Laminin EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02136504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GM50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004618-RA">
        <property type="protein sequence ID" value="MESCA004618-PA"/>
        <property type="gene ID" value="MESCA004618"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1047969_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000193">
        <property type="entry name" value="Laminin subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF435">
        <property type="entry name" value="LAMININ SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00052">
        <property type="entry name" value="Laminin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00281">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51115">
        <property type="entry name" value="LAMININ_IVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin EGF-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="7">
        <location>
            <begin position="26"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="8">
        <location>
            <begin position="134"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004618-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30185" checksum="AE2567A6A94C9C70" modified="2013-10-16" version="1">PGVTGDKCDRCAANHYQFGPHGCQPCNCEVKGSLDNVPSCDPESGICLCKDNVEGRRCRECRPGFFNLDLDNRFGCTPCFCYGHTSECQSALGFSQVSMNSNFNRNQERWQALDYEDEIYRXFSQVSMNSNFNRNQERWQALDLNGIPADLKYNQFSQSIATVANGKDNIYFIAPDRFLGDQRASYNKLLKFKLQLVGQTAPNPNAQDVILESGSKKIALPIFAQRNSIPDHNVKEYVFRLHEHHDYNWQPSQSAKSFMSILANLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMG4</accession>
    <name>T1GMG4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMG4"/>
    <dbReference type="STRING" id="36166.T1GMG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004738-RA">
        <property type="protein sequence ID" value="MESCA004738-PA"/>
        <property type="gene ID" value="MESCA004738"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034788_7_1_1"/>
    <dbReference type="OMA" id="VCQSIFG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF28">
        <property type="entry name" value="P3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004738-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="30202" checksum="DD55906434361EDD" modified="2013-10-16" version="1">MRGILSRPIGPAIGFIGQFLVMPLVSFGLGFAVFSASPELHLGLFFTGVSPGGGASNMWSVILGGNIDLAIIMTTVSNLASFAMMPFWIFTLGKVIFDRANLGVPYSRIASFAVALLVPLGIGILIQYYLPRVKRILVRLLKPISMLLILFIIIFAIVTNLYLFELFSWQIIVAGLGLPWIGYIISWIAAKLLKQSPSDALTIAIETGIQNTGISIALLKFCLEQPAADLTTVIPVSVAIFTPIPLLVLYIFQKCKRDGTNGERRKILATEAKENGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMP1</accession>
    <name>T1GMP1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007264">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004819-RA">
        <property type="protein sequence ID" value="MESCA004819-PA"/>
        <property type="gene ID" value="MESCA004819"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3377601_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004819-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="34" mass="4061" checksum="9046B45A609C48B4" modified="2013-10-16" version="1">MRSISRSVSWRKEDELSSRKRRSGKSPIILRLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMR3</accession>
    <name>T1GMR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fido domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMR3"/>
    <dbReference type="STRING" id="36166.T1GMR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004842-RA">
        <property type="protein sequence ID" value="MESCA004842-PA"/>
        <property type="gene ID" value="MESCA004842"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040460_3_2_1"/>
    <dbReference type="OMA" id="AYSHHEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="entry name" value="Fido-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="FIDO DOMAIN-CONTAINING PROTEIN DDB_G0283145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504:SF34">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE FICD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="Fic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fido" evidence="12">
        <location>
            <begin position="48"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="130"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="162"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="11">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004842-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="234" mass="26779" checksum="AC8D61463240465E" modified="2013-10-16" version="1">MTLAQTRSILETRMAVDGKSIDEHNEILGMDLAMKYINATLVNDNDYISMKDILEIHRRVLGHVDPIEGGEFRRTQVYVGGHIPPGPSDLTILMQRFEQWLNSESVVTMHPVKYAALAHYKLVHIHPFIDGNGRTSRLLMNLILMRAGYPPAIIPKQQRHNYYNYLQLANEGDIRPFVRFIADCTERTLDIYLWATSDMQKQIPLLELEQSDFSLMNQENDMESGSGTNLNSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMS5</accession>
    <name>T1GMS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GMS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004855-RA">
        <property type="protein sequence ID" value="MESCA004855-PA"/>
        <property type="gene ID" value="MESCA004855"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005350_1_4_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000015">
        <property type="entry name" value="Small G protein signaling modulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000012">
        <property type="entry name" value="Small G protein signaling modulator 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF13">
        <property type="entry name" value="RUN AND TBC1 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="71"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004855-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="374" mass="42726" checksum="2EFBF057E76D3AB4" modified="2013-10-16" version="1">MGYLDRNGSETPREESLNMLGIPFMEEPSQRQEWIAHLEFSHNKEACELTWDNVDVMLPRTEKLRNMVRNGIPHSLRPQMWMRLSGALNKKIKSEVSYEEIIKASENDALMTSKQIEKDLLRIMPTNACFSNAKGTGIPRLRRILRGLAWLFPDIGYCQGTGVIVXXXXXXXXXXXXXXXXXXXXXXACLLLFMEEENAFWMMATIVEDLLPASYYASNLIGVQADQKVLQTLISNYLTSVNDSLKTHDIELSLITLHWYLTLFANVIHMKILVRVWDWFFYDGSIVLFQLTLGMLKMKEEQLKDLENSAQIFNCLSDIPGEIDDVETLFELALEVGGSLSQTKFLEKTPAFRQPQNGDFSTYPSDNSESEDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN97</accession>
    <name>T1GN97_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/potassium-transporting ATPase subunit beta-1-interacting protein</fullName>
            <shortName evidence="7">Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NKAIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02191578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005041-RA">
        <property type="protein sequence ID" value="MESCA005041-PA"/>
        <property type="gene ID" value="MESCA005041"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_898872_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008516">
        <property type="entry name" value="Na/K-Atpase_Interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084:SF6">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084">
        <property type="entry name" value="T-CELL LYMPHOMA BREAKPOINT-ASSOCIATED TARGET 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05640">
        <property type="entry name" value="NKAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005041-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="310" mass="35505" checksum="67B12E3E308C74C5" modified="2013-10-16" version="1">RSLKCLKFKQLQKEFDWYIIWCLIWIGWNSFLICFYLNIGVLNNESSDILNFGTGSYSWFVAYGPGCQPADSNNNTNGDPSSLYRPEVEHCILDYEIVEIIHSVKHVNNKSKHRQSLYSIEFGPSIDSLRSCRSDTILDYDDNILDDRQLSPKPMTPRRVKRRSVMTRGTNNRQSTTSRRSQSNTNTNRNSTRSSRRKYYNQNPVTKLMEQQIQIQQKSQKKLASFRNTNNTTTLTTSNLLSFNSDMANLPPPPPLLEKKSSTYNSFPQNIAPDPIYYNMGHMTIPSASGGHYNPTYQHSTSNLANNGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNL4</accession>
    <name>T1GNL4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005171-RA">
        <property type="protein sequence ID" value="MESCA005171-PA"/>
        <property type="gene ID" value="MESCA005171"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592493_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005171-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9266" checksum="EF822EFED728998E" modified="2013-10-16" version="1">MIAAIENQLQFENLRFNFKSICQMKVSAAQVRNNSSYLKNRSRNQVSSPQSGRTIELLHIYSTLLRENLLRWHASSKVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNV3</accession>
    <name>T1GNV3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095346">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNV3"/>
    <dbReference type="STRING" id="36166.T1GNV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005264-RA">
        <property type="protein sequence ID" value="MESCA005264-PA"/>
        <property type="gene ID" value="MESCA005264"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136304_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12493" checksum="7B9B3CD191B8F5C1" modified="2013-10-16" version="1">MRDKLSQFFCLQRNARKFNSDSEGHNNVPIKNTINGSTYTNGHVGATEGGPETTETDPSGTGRMRKSMERNGSTQNDSIHLKRRVGLFSGVALIVGTMIGEYTVEKGFMVYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP49</accession>
    <name>T1GP49_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP49"/>
    <dbReference type="STRING" id="36166.T1GP49"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005366-RA">
        <property type="protein sequence ID" value="MESCA005366-PA"/>
        <property type="gene ID" value="MESCA005366"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1257408_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41142">
        <property type="entry name" value="SI:DKEY-16J16.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41142:SF1">
        <property type="entry name" value="SI:DKEY-16J16.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005366-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="206" mass="22674" checksum="07D965B13022FE2E" modified="2013-10-16" version="1">MSLGAMNSENCKEKSTSSPPTPAQLFDSSMLLCQTNLLCDHHSSEEELEVINGPCSGNEVNDGQQNLHSLPSEDELFGDDDGSGDNHHNHHHQDQEGKVLSSSMSPSRPRSNMLEIRKRSLAHSLTKNSSSAGAGFAGGSGGSATLDLEVRSVSPPAKLFHCAISPKRRPMRTHHAPQRLQRPHRPCLDFDKMQQSYFKLLNNFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP66</accession>
    <name>T1GP66_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Myosin-VIIa</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02008201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GP66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005383-RA">
        <property type="protein sequence ID" value="MESCA005383-PA"/>
        <property type="gene ID" value="MESCA005383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_14_1_1"/>
    <dbReference type="OMA" id="TGFQGRC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016461">
        <property type="term" value="C:unconventional myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17092">
        <property type="entry name" value="FERM1_F1_Myosin-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd13198">
        <property type="entry name" value="FERM_C1_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13199">
        <property type="entry name" value="FERM_C2_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01381">
        <property type="entry name" value="MYSc_Myo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000012">
        <property type="entry name" value="Myosin VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000013">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000079">
        <property type="entry name" value="unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000036">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057130">
        <property type="entry name" value="Myosin_VII_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041793">
        <property type="entry name" value="MyoVII_FERM_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041794">
        <property type="entry name" value="MyoVII_FERM_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036106">
        <property type="entry name" value="MYSc_Myo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF33">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21998">
        <property type="entry name" value="FERM_C1_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24123">
        <property type="entry name" value="Myosin_VII_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 12" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="68"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="599"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="15">
        <location>
            <begin position="883"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="14">
        <location>
            <begin position="1109"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="1392"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="15">
        <location>
            <begin position="1539"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="14">
        <location>
            <begin position="1689"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="12">
        <location>
            <begin position="571"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="161"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1983" mass="230382" checksum="19392F00B09F8D2E" modified="2013-10-16" version="1">MLLYRATNHDRGDYIWIEPISGVEFDVAIGARVTSTEGRRIQVRDDDDNEIWLTPERRIKAMHASSVQGVEDMISLGDLHEAGILRNLLIRYLDNLIYTYTGSILVAVNPYQILPIYTADQIKLYKERKIGELPPHIFAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAISGKHSWIEQQILEANPILEAFGNAKTIRNDNSSRFGKYIDIHFSNNGVIEGAKIEQYLLEKSRIVSQNPSERNYHIFYCILAGLSHDEKKRLWLGHASDYKYLTGGDCITCEGLDNLDATEIPERINVERVAELLGLPYQHFVDALIRKTLFAHGETVVSTLSREQSIDVRDAFVKGIYGRLFIHIVKKINSTIYKPKQSSRNAIGVLDIFGFENFNHNSFEQFCINYANENLQQFFVQHIFKLEQEEYNHESINWQHIEFVDNQDALDLIAIKQLNIMALIDEEAKFPKGTDHTMLAKLHKTHGSHKNYLKPKSDINTSFGLNHFAGIVFYDTSGFLEKNRDSFSADLLQIVSQSKNKFLRHIFATDIEMGAETRKRTPTLSTQFKKSLDSLMKTLSMCQPFFIRCIKPNEYKKPLIRRAGYPIRHGYRDFVERYRFLISGVPPAHRTDCQHATARICAMILGKSDYQLGNTKVFLKDAHDLFLEQERDRVLTRKILILQRSIRGWVYRRRFLRMRAAAIMVQKHWKGYAQRNRYKKMKVGYMRLQALIRSRVLSHRFRHLRGHIVGLQAHIRGFMVRREYGHKMWAIVKIQSHVRKMIAMRKYKKLKEGYKQFNEVLQLRRIEEHQLKQKGNKRAREIAEQHYRDRLHELERIDMEKEIEERRADIIGQIHISEDEEDLSEFKFQKFAATYFQGNVTHQYSRKPLKHPLLPLHTQGDQLAAQALWVTILRFTGDLQEPRYHQTERDRSLDTTSVMSKVTATLGRNFIRSKEFQEAQLMGLDPDTFLKQKPRSIRHKLVSLTLKRKNKLGEDVRRRLQDEEYTADSYQSWLESRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLSNNPTKSSHARGWILLSLCRRSSWICSIFEDRLKRTFNNGTRNQPPSWLELQATKSKKPIMLPITFMDGNTKTLLADSATTARELCNQLSDKIALKDQFGFSLYIALFDKVSSLGSGGDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEIFAPWHDPTVDQVATNLIYQQVVRGVKFGEYRCDKEDDLAMIAAQQYFIEYGTDMSMERLFTLLPNFIPDFCLTGIEKAVERWATLILQAYKKSYFIKENVAPLQIKEDIVNYAKYKWPLLFSRFYEAYRNSGPNLPKNDVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQKTNKVFTQTFSLSTVRGEESRYTIALQDYRSPGESTTFLSFLKGDLILLEEDSTGDCVLNNGWCIGRCERTQERGDFPAETVYVLPTLTKPPIDILALFNVDEAHHGRRLNMITNGINGHGTEPRERPHTLMEYAIDHFRLPPKRTMSKTLTLGSKRGHDDIWRYSREPIKIPLLRKLQSKEELAEEACFAFAAILKYMGDLPSKRPRMGNEITDHIFDAPLKHEILRDEIYCQIMKQLTDNRNRVSEERGWELMWLATGLFACSQGLLKELSLFLRTRRHPISQDSLHRLQKTIRNGQRKYPPHQVEVEAIQHKTTQIFHKATFPMTPMKLSKLIVPPEQKTFVITYHNVPEGDFFFDFVRHLTDWIKKARPVRDGSNPQFSYQVFFMKKLWTNTVPGKDRNADLIFHYHQELPKLLRGYHKCSREEASKLAALVYRVRYGENKSELQSIPQILKELIPTDLMKMMSSSEWKRHIVASYNQDAGISSDDAKIAFLKIVYRWPTFGSAFFEVKQTTEPSYPELLLIAINKHGVSLIHPLNKDILVTHPFTRISNWSSGNTYFHMTIGNLVRGSKLLCETSLGYKMDDLLTSYISLMLTNMNRNRMLRPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP78</accession>
    <name>T1GP78_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154813">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005398-RA">
        <property type="protein sequence ID" value="MESCA005398-PA"/>
        <property type="gene ID" value="MESCA005398"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742956_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005398-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7953" checksum="C1B0D0479183AE72" modified="2013-10-16" version="1">MSTINSTDYGNQHYASDKKTEKISSIPGNVMEKYRLRLEDGKDLDKRRTAAHISVMSSTTSIPGTIRLCHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPP4</accession>
    <name>T1GPP4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GPP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005580-RA">
        <property type="protein sequence ID" value="MESCA005580-PA"/>
        <property type="gene ID" value="MESCA005580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_346349_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
        <property type="term" value="P:non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048935">
        <property type="term" value="P:peripheral nervous system neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002225">
        <property type="term" value="P:positive regulation of antimicrobial peptide production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035206">
        <property type="term" value="P:regulation of hemocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008063">
        <property type="term" value="P:Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF25">
        <property type="entry name" value="DORSAL-RELATED IMMUNITY FACTOR DIF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHD" evidence="2">
        <location>
            <begin position="141"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="RHD" evidence="2">
        <location>
            <begin position="657"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="816" mass="91536" checksum="413EEB3C95D4947C" modified="2013-10-16" version="1">SNNSSPCKSPSKLNKSGGRPVGRSVSSVSGKRPNLQPDIIHIRLKGEDDYNNEAYSTASTLTLNHLDRKTVSALQLADIPITEGNMGLVAIADAQSLKNLCEGQYGVQLDPSVDLTEAEHFALYTAIPPQATTNPDSLKRKRQKINDTVHEHIFRQYQNEPQDTGYSHKLQPSSIDLNAVRLCFQVFIKSSSGKLNLPLKPVVSDIIYDKKAMSDLVISALCSCSSTVDGGKDIILLCEKVVKEDIEIRFFEEKTGWEAKGDFQHTDVHKQTAIKFRTPRYRTLDIDKPVQVSIQLRRPTDGVTSEPLQFDYLPLDTGKRTFWMMRRHLKRKPEVDIFQRILSEDSEANKLTLQEKDSEDSTAGTPFSVEDYQICVPPLKHSRSEEDDSYEEEIRNKEREVDKIVQEKMLEFEKAFNETDFNTNEVDTQTASSLLRKQSLIENKLRPLEQNDKISVCDPTESSSMSGRDNALNDLLDQVAELDEIYTDHILKRNTDANEITQIEDLNRKVNKIDSVDFDDAATYTSLQFAFKNPLPMSDIMPPTPPGSNSAPDDLTYDPIDISLTLAQTPVIEVSSVDKSEPLPPLPPKRVKKPQQQSFPSPTVTEINSNIIRDDIEMNPPNLPQAPNGFVDRQVGVSQPSSISQSSQVPPDSARIQQTAYVRIVEQPASKALRFRYECEGRSAGSIPGVNSTTENKTFPTIEIVGYKGRAVVVVSCVTKDAENGKYKPHPHNLVGKEGCKKGVCTLEVNSDTMRVVFSNLGIQCVKKKDIEAALKAREEIRLTHLSIIXDPFINNCHNLFANMFFYMENQKHRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQC6</accession>
    <name>T1GQC6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005830-RA">
        <property type="protein sequence ID" value="MESCA005830-PA"/>
        <property type="gene ID" value="MESCA005830"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029154_1_0_1"/>
    <dbReference type="OMA" id="CDILRCD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21385:SF0">
        <property type="entry name" value="RE51073P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21385">
        <property type="entry name" value="ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588459" description="C2H2-type domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005830-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="518" mass="59669" checksum="EE4D9CC6D993E256" modified="2013-10-16" version="1">MSSKRYGLEITLLYLLLLVPWPQNKNVVKMFCSKDNSRLVRKIIKAKWTPILEKYQVKFPLECPLHPLRDIFGPRQDAKKRHRPTQWTCKLCGKSFYQEKFVDNHFDVRHKHLINKAEDSVCLSEFCDIMRCDVFQSEESKLSGSDSAISTDIEVWGSSAFGKLSHSSAMVSKTNQNLSPIRDSTVLTGGSKKHQNRHVCPEEINSQTKTNSKNAKESVERKKEDHIPQIENKSDSTQEEQKVIMNSSKNDSPTADATIENYINMSKIKASCQSEELAKLKTKCEFLVRSCIGSLLLKMSDEKFKEMEAELNKAICWYLSCERYWEDGPLEPRAFPWGLMVIVIFVLSLGTCFCYYIIWILFELKDYIKNSSYQQSRRHKHHLLYQHHNLPPQIKQYPDYHPATMGGMGPGAALGHHIDPLYDYRHSPRYIPQHHIQSNTMEMMETSSTNSMMGIQAAPSSSAPLMPPPLIIQDSDLSNKDSSILRSTDLNEISHNEHYIYVTYPPDLKNRLYDKYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQM9</accession>
    <name>T1GQM9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005940-RA">
        <property type="protein sequence ID" value="MESCA005940-PA"/>
        <property type="gene ID" value="MESCA005940"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1951236_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005940-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14837" checksum="6CE14E42E5C6F5C4" modified="2013-10-16" version="1">MNPHPFFSKAPTFASAPPSPSTLHGQLKYGNGNVNINDQHRHQFSQYNNEQIKQHFNADIEGRSQSVPLHYKNNSNSPIFINTNYNEYSSACNSIAHTPVPHEFNDLEMFSEDAQQQNLWLLDRCHRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQW3</accession>
    <name>T1GQW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQW3"/>
    <dbReference type="STRING" id="36166.T1GQW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006028-RA">
        <property type="protein sequence ID" value="MESCA006028-PA"/>
        <property type="gene ID" value="MESCA006028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445032_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="614" mass="69926" checksum="0CF9A6A0CD61F4D3" modified="2013-10-16" version="1">MQIKQPQLTLQYPNLSKKLNNFQDTYRTYIQELPLWTENETIINTPAKAIEVTEDVIVNVSPTDPISQDKLVQAKKRHSGAFINSKKDILKRAKEVKLLKKPPPIIVNPVNTPLPKIANMVGQIATESVPPITVCNFAKETLPTLADFHTVNDCLNLGSVIKDKDLLKLILKALKWPYDKHTLNAQIQRLKCTRFRDIMVDPLLLQDTDLIQILTQYFGPNFKFQQPPQKPMESSSRKSPPPLQPILPQSLTYKLPAETSIQVINVPKSTSSQKRRKKHLKTTDVIVLSDDENEGSKKAESMEEKRPRKRKQSLTSLQSKNNIDDLVIMGQIPPNCSKAVPHTVLSKTPQITYKKRPKVKQSEVVLNMNEYDDENPLVIDDSSVDIWENVKIDETAPINQVDHLIGEATKIVEIEIPENEKQFSEIDSTTLVTNLTTSTDISKKLNLPPADVLIKDSLKFDESEGEDVDVLGFELQQTEIDKKLENKIVIEEIKKVQISEESTENLIENGKQNKDKDESEEWQVLPKRRRLLKPEKNKEKELKDPPQKVAEDQLKSRTKDQKKLPEPAPITNSRPARKSKTFSKYYKDPKSVENSTQPTVRTSSRSTKGTSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRH8</accession>
    <name>T1GRH8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proline dehydrogenase</fullName>
            <ecNumber evidence="5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the proline oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02000425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRH8"/>
    <dbReference type="STRING" id="36166.T1GRH8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006263-RA">
        <property type="protein sequence ID" value="MESCA006263-PA"/>
        <property type="gene ID" value="MESCA006263"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018202_3_1_1"/>
    <dbReference type="OMA" id="GPLKKYH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="104"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006263-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="509" mass="58704" checksum="F12EC30229B8D09E" modified="2013-10-16" version="1">MSFRKLWGTISNKISTKKSSPRIDLTFNDPEASFRSKTTPEVLRAYIVYKLCSYEKLVDHNMKLMKISEKILGKFLFSKLMKATFFGHFVGGEDLKQLMPFVMRLQSFGVKSILDYSVEEDLSEEQAEKIVKESSVSEMGDEIADGSLKQYHVMENSHMDRLGTGITAIKLTALGRPQLLLQISEVIMKAREYIDSMAGGNHKNVLLHHKTIEEMERYYKDKVGEQQDVKEFLKQVTSDKEGIINLFPWSGIVDENFQLSETFRIPDPKTGRMRKFISQITPKEESMFRNMIHRVNTIAQKAKEVGGCVMVDAEQTYFQPAISRITVEMMRKHNKEKVVVMNTYQNYLQSAFDEVKADLEQAKRQNFYFGAKIVRGAYMEQERARAKELGYPDPINPTYEATSEMYHKTLIECLTRIRDLKKQKMDPKRITIMVASHNEDTIRLAINKMDEIGVSPEDNVILFGQLLGMCDFITFPLGAADMMSTSIYHMVLLQKCFLICREEPLRIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRV9</accession>
    <name>T1GRV9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006403-RA">
        <property type="protein sequence ID" value="MESCA006403-PA"/>
        <property type="gene ID" value="MESCA006403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690648_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8492" checksum="875D42B07FDD94B1" modified="2013-10-16" version="1">MMMVTFEFFIEHTITFLTPLVSSLSKTSLTLHCWKAIVCESNVLQVSLLIRKILTKLSLNLFNSLEIKFSSKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRZ6</accession>
    <name>T1GRZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">omega-amidase</fullName>
            <ecNumber evidence="2">3.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Nitrilase homolog 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutaramate + H2O = 2-oxoglutarate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:32963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16769"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:32964"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2-oxosuccinamate + H2O = oxaloacetate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:59412"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57735"/>
            <dbReference type="EC" id="3.5.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:59413"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="3.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02034579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRZ6"/>
    <dbReference type="STRING" id="36166.T1GRZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006443-RA">
        <property type="protein sequence ID" value="MESCA006443-PA"/>
        <property type="gene ID" value="MESCA006443"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_0_1"/>
    <dbReference type="OMA" id="GTYSKIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050152">
        <property type="term" value="F:omega-amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF30">
        <property type="entry name" value="OMEGA-AMIDASE NIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00036637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006443-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="17530" checksum="B6B230BF7FA49AC7" modified="2013-10-16" version="1">MSLRLALLQLKFTKDKIANVKNAVEKISKVVKESQPRIVALPECFNSPYGTKYFPEFAEIIPGGYTSEILSKCAKDNGIYIIGGSIPEKDNDKIYNTCTVWDPSGALVAKHRKVHLFDIDVKGGIRFKESETLSPGQDLTIVEIDGHKIGVGICYDIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS47</accession>
    <name>T1GS47_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS47"/>
    <dbReference type="STRING" id="36166.T1GS47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006495-RA">
        <property type="protein sequence ID" value="MESCA006495-PA"/>
        <property type="gene ID" value="MESCA006495"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="OMA" id="TANEIEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006495-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="336" mass="38305" checksum="846C0124A4F41046" modified="2013-10-16" version="1">MISIGEITYDESKILGEGAFGKVYDGRFRLEKAAVKKVKFNQVSLKKVLEEARILRTLCCSHPNIIRMFDVQADKNHIYICMEYCDGGSFEDFLSAQKLTEEMAKYFIPQICKGLEALHLNKIVHRDLKPDNILLKFQNVTGNLEIILKISDFGVSRFILDGMNANTFAGTEVYMAPEVLSYVLYNEMCDMWSVGIILFKGLTGQFPFTSLTYLVNAFRQKKELFIENKLKSYTISQALKDLIQKLLIVNPHKRLTVNEMANHNYLEECYRSNVQTGFVMDWYSQRNSRTKLQTSQIFGMSPGLAFGLGIAALGLLGFGIYNINKPSDSRKQAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSS4</accession>
    <name>T1GSS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006735-RA">
        <property type="protein sequence ID" value="MESCA006735-PA"/>
        <property type="gene ID" value="MESCA006735"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925244_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006735-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7293" checksum="5664997C2E78BC06" modified="2013-10-16" version="1">MDFSCHPTNHEFGVDFAMDAYQEKVMHARYKSQIFLHYYNLRSCSHGWITMKSWISSTTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT07</accession>
    <name>T1GT07_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02026202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT07"/>
    <dbReference type="STRING" id="36166.T1GT07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006825-RA">
        <property type="protein sequence ID" value="MESCA006825-PA"/>
        <property type="gene ID" value="MESCA006825"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125101_1_1_1"/>
    <dbReference type="OMA" id="RHSKHNI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097401">
        <property type="term" value="P:synaptic vesicle lumen acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="9"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006825-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="12372" checksum="237FAA7B1D344C0A" modified="2013-10-16" version="1">MRCFRKRTVEKIEEARKRKARRLKQAKDDAIEEIEKYRQERENQAKHIESREEVAAKMEADTRDQLADIDRSLASHKEPVIQELLQYMYNIVPKMHKNYKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT49</accession>
    <name>T1GT49_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Vta1/callose synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT49"/>
    <dbReference type="STRING" id="36166.T1GT49"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006870-RA">
        <property type="protein sequence ID" value="MESCA006870-PA"/>
        <property type="gene ID" value="MESCA006870"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030378_1_0_1"/>
    <dbReference type="OMA" id="AYWCEYH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041212">
        <property type="entry name" value="Vta1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18097">
        <property type="entry name" value="Vta1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="10">
        <location>
            <begin position="52"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Vta1 C-terminal" evidence="11">
        <location>
            <begin position="288"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="195"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006870-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="37267" checksum="7BF2C88C54320EBE" modified="2013-10-16" version="1">MFLRSHQSTDDFTAVVAKKYGRVMSYLHWKGVNKVCKNECRFPQCPNSLKPIQHFMKIAQEHDERDPVVSYWARLYALQQGLKLKTNQPDETAFLMAIMDWLETLKKSTDNEAISNEVAAQAHIDNYALKLFLYADKQDREMNYGKNVVKAFYSCGVLYDILQTFGELTEEAANNRKYAKWKAAYIHNCLKNGETPVPGPMVDEEGEGDNEAAGENPEADHESPEQPTTENISPPSAEDVLNDPDKLPSPPQEDESPSDFKPFIPEVKPELKPFTTPVPLENLKLTDEQMTKAQKYCKYAGNSLNYDDVKAAVEYLQKALNLLTTGTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTJ7</accession>
    <name>T1GTJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M1 membrane alanine aminopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02046328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTJ7"/>
    <dbReference type="STRING" id="36166.T1GTJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007031-RA">
        <property type="protein sequence ID" value="MESCA007031-PA"/>
        <property type="gene ID" value="MESCA007031"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064126_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF294">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE-DEGRADING ECTOENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007031-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13710" checksum="0BFBFF42D186ACFE" modified="2013-10-16" version="1">MENWGLITFRDSSLLVPDEEDKMSSAQHTEYVASIISHEIAHQWFGNLVTPKNWEDLWLKEGFASYLSTLAIHNIYTNWRRDPAFHLCAVIKLEAINGYTFTESCRYLAFSSFACNPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTR8</accession>
    <name>T1GTR8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTR8"/>
    <dbReference type="STRING" id="36166.T1GTR8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007109-RA">
        <property type="protein sequence ID" value="MESCA007109-PA"/>
        <property type="gene ID" value="MESCA007109"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760708_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007109-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7975" checksum="DFA477301E359DE7" modified="2013-10-16" version="1">MELQGHLFYPKLITAEEPHHSRVSCKTSLTMGDDKDIDAEIEDDLLEGNEYQLVLPNGNVIGHRSLNRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUW1</accession>
    <name>T1GUW1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007533-RA">
        <property type="protein sequence ID" value="MESCA007533-PA"/>
        <property type="gene ID" value="MESCA007533"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_895134_0_0_1"/>
    <dbReference type="OMA" id="LAYNSHY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007614">
        <property type="entry name" value="Retinin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931:SF4">
        <property type="entry name" value="GEO13385P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04527">
        <property type="entry name" value="Retinin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007533-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="311" mass="32838" checksum="BC0AFF538AC7839A" modified="2013-10-16" version="1">MQISNISNLISLLAVVEDCLSYQFSFCSYLIQPAQYVQIITFFAILAIAAAKPGFLSQPLVSTYEAAPIVSTYVKSVPTSVSHSSSSVVHSSAHIAEPIIAPVVKTHLTTAPVIKSYATPIVKSYAAPVVYSEPLSYHHSAPVAYSAPLTYASGYSSCRESGPSGFCMFHLGGSISEDEFCGAGNSSFTLFAIVAAVAAKPGYIGEPIVSTYAAAPVVTSYVKSVPTSISHSSSSVVHSSAHIAQPVVAPVVKTHLTTAPVIKSYVAPIAHTYSAPLSYSSYHHDSPLAYNSHYGYDHGYGHGYGHGYSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVN6</accession>
    <name>T1GVN6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111491">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVN6"/>
    <dbReference type="STRING" id="36166.T1GVN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007848-RA">
        <property type="protein sequence ID" value="MESCA007848-PA"/>
        <property type="gene ID" value="MESCA007848"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3371108_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007848-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="35" mass="3893" checksum="02D09A3534AE6D16" modified="2013-10-16" version="1">MDSLVQDKIYKRISVVDFQGFCSDSNNPFTAAAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWF4</accession>
    <name>T1GWF4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008129-RA">
        <property type="protein sequence ID" value="MESCA008129-PA"/>
        <property type="gene ID" value="MESCA008129"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834093_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008129-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7146" checksum="3F21CA8C23E73A86" modified="2013-10-16" version="1">MEAKPAILPTIILPTISPITRIPTTLPTKPAPCNCDRFCDPSISQQISARDLGRIATFAIQTNVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX24</accession>
    <name>T1GX24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008362-RA">
        <property type="protein sequence ID" value="MESCA008362-PA"/>
        <property type="gene ID" value="MESCA008362"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433182_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577220" evidence="1">
        <location>
            <begin position="18"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008362-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10688" checksum="8890FB09640FA362" modified="2013-10-16" version="1">MSFVKTIFLLILSSALCILLCFLANPNEVHDICQAEAQIIRKKWYIEYVKECVQDDRRFDLEENLIHIQNCQIDQSLTSYDQCLLDNGVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXB8</accession>
    <name>T1GXB8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Ubiquitin-like protein ATG12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ubiquitin-like protein involved in autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a conjugate with ATG5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ATG12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXB8"/>
    <dbReference type="STRING" id="36166.T1GXB8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008463-RA">
        <property type="protein sequence ID" value="MESCA008463-PA"/>
        <property type="gene ID" value="MESCA008463"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106795_3_0_1"/>
    <dbReference type="OMA" id="YAKTHAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01612">
        <property type="entry name" value="Ubl_ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000117">
        <property type="entry name" value="Ubiquitin-like protein ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007242">
        <property type="entry name" value="Atg12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385">
        <property type="entry name" value="AUTOPHAGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04110">
        <property type="entry name" value="APG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008463-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="15109" checksum="84C71BBD2AD5EF23" modified="2013-10-16" version="1">MSDPTEEPEQPQSVQTEEAASPTTEEPQKPPSEPEPQHYHKTIKVIDILLNATGNVPIMKKRKWAVDANKPIMWIMQFIHKYLKLDAEEQIFLYVNQTFAPAPDQLVRNLYECHGTNGKLVLYYCKNQAWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXQ9</accession>
    <name>T1GXQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02167437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXQ9"/>
    <dbReference type="STRING" id="36166.T1GXQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008613-RA">
        <property type="protein sequence ID" value="MESCA008613-PA"/>
        <property type="gene ID" value="MESCA008613"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_8_1_1"/>
    <dbReference type="OMA" id="ANIRCPL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF14">
        <property type="entry name" value="SERINE HYDROLASE-LIKE PROTEIN DDB_G0286239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="23842" checksum="DE857BC93577A6C2" modified="2013-10-16" version="1">MLEKDENLAIYCIDLPGHGKSSHYPKGMQIVKKHGWEKVTLMGHSLGGALTFMYAASFPNEVEKLINIDIAGPTVRSQLKHAEATGFAIDKYLQYENLPESKLPCYSYDEMVELVLDAYDGSVDLPSVKILMERGMSSAPEYLHKNGFHFSRDLRLKVSLLGMFTEEQVLIYAERIKCKVLNIRAEPGMKFERYDVYEQVIEKLRNNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXV8</accession>
    <name>T1GXV8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXV8"/>
    <dbReference type="STRING" id="36166.T1GXV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008669-RA">
        <property type="protein sequence ID" value="MESCA008669-PA"/>
        <property type="gene ID" value="MESCA008669"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121178_0_0_1"/>
    <dbReference type="OMA" id="NECKGTE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CR-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="37"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008669-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="15891" checksum="8DE5DF4943A2DAA4" modified="2013-10-16" version="1">MFFGGGGSGFGGRGRRERRGKDLVHQMAVTLEELYNGAVRKLALQKNVICQGCEGRGGKKGSVKKCQQCRGNGIEIKMHQIGPGIVQQIEQVCRKCMGQGEIIPEEDRCKTCNGKKTTRERKILEVNVEKGMRDGQKIVFHDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYH3</accession>
    <name>T1GYH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ENT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02115931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYH3"/>
    <dbReference type="STRING" id="36166.T1GYH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008900-RA">
        <property type="protein sequence ID" value="MESCA008900-PA"/>
        <property type="gene ID" value="MESCA008900"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976052_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.40">
        <property type="entry name" value="ENT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033482">
        <property type="entry name" value="EMSY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005491">
        <property type="entry name" value="ENT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036142">
        <property type="entry name" value="ENT_dom-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16500">
        <property type="entry name" value="BRCA2-INTERACTING TRANSCRIPTIONAL REPRESSOR EMSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16500:SF3">
        <property type="entry name" value="BRCA2-INTERACTING TRANSCRIPTIONAL REPRESSOR EMSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03735">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01191">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158639">
        <property type="entry name" value="ENT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51138">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENT" evidence="3">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008900-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14529" checksum="D1157603E029BE5C" modified="2013-10-16" version="1">MWPRKLDLNQDECNSMLRQIELDCYSSVITAFRAQGGLDDLKTRILADLRKIFHIDEDRHKAEARRVANDEELTTIADLVFGESASSEWRNEGRRTNGLLQRAPAQTAFCKMSNNIKEDATNMNSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYR9</accession>
    <name>T1GYR9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-50 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02379113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYR9"/>
    <dbReference type="STRING" id="36166.T1GYR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009006-RA">
        <property type="protein sequence ID" value="MESCA009006-PA"/>
        <property type="gene ID" value="MESCA009006"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962095_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007881">
        <property type="entry name" value="UNC-50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841:SF6">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05216">
        <property type="entry name" value="UNC-50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009006-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="15503" checksum="0BD1A7EE731F3EF5" modified="2013-10-16" version="1">MKDNFVLIRRLKRKISHKKYEHFFVFTCSFDELWIPTTITAASSATSNNICLSATTKTYKFFRKLFKFQQMDFEFAMWQCINLFIAPQKVYRNFNYRKHTKSQFARDDPAFLVLLLACMFDLLYYIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ51</accession>
    <name>T1GZ51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02101715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ51"/>
    <dbReference type="STRING" id="36166.T1GZ51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009145-RA">
        <property type="protein sequence ID" value="MESCA009145-PA"/>
        <property type="gene ID" value="MESCA009145"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392232_0_0_1"/>
    <dbReference type="OMA" id="ANCVIRR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009145-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12576" checksum="BF89C297B3BE2356" modified="2013-10-16" version="1">SYDFKCGGSLISPRYVLTAAHCLSNTLYLLKYNFKCFFSLNRISVRLGEHTLDNDEDCRVSPSGRRRCAPPVKDVSIEMQIKHPQYDKNKKINDIALLRLSESIPLNDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZH7</accession>
    <name>T1GZH7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02379112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZH7"/>
    <dbReference type="STRING" id="36166.T1GZH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009278-RA">
        <property type="protein sequence ID" value="MESCA009278-PA"/>
        <property type="gene ID" value="MESCA009278"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1808403_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046620">
        <property type="term" value="P:regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF418">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE WARTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009278-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="16924" checksum="0CA0DAFAC80E693B" modified="2013-10-16" version="1">MMKINLAPQAQVEMRKMLNQKESNYIRLKSAKMDKSMFVQIKAIGLGGIWGADVVKRNQVAHVKVERDILAEADYEWVVKLYYSFQDRDNVYFVMDYIPGGDLMSLLIEKEYYQQIGDHARQNSMEPWEDFGERSQQFWKDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZS4</accession>
    <name>T1GZS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035529">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009379-RA">
        <property type="protein sequence ID" value="MESCA009379-PA"/>
        <property type="gene ID" value="MESCA009379"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515245_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009379-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9704" checksum="BF72AB9C4FE22E53" modified="2013-10-16" version="1">MEYGELDPDFDMKLIEASITFKLALKSSAGMSTEPVYDDIEFHVSCPNDRLNDCVQMTLVQMSIIQMMSSRFLTSCTNHKNTIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZW0</accession>
    <name>T1GZW0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074808">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZW0"/>
    <dbReference type="STRING" id="36166.T1GZW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009418-RA">
        <property type="protein sequence ID" value="MESCA009418-PA"/>
        <property type="gene ID" value="MESCA009418"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079802_0_0_1"/>
    <dbReference type="OMA" id="LNRINCH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009418-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="25390" checksum="DB86C0400EBDEE31" modified="2013-10-16" version="1">MFYYFYPGLNDPLNRINCHLASVIRSKFIKEYKNARCLASLVKELFSLFVDGVNFEINGKITNVKFVLGLIIGDNLALNGILDFIIGFQLRNRENYERDVLLNDSSKTGIENVSMFNILPYFHCTLNLSLDLMHDFFEGIFQYDICQAVLYFIRKKYFTLTELNERINNFAYGKEDENNLKMTSREAWQFLYLLPIYIGDKVDPHDEVWKLIKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H035</accession>
    <name>T1H035_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SERTA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H035"/>
    <dbReference type="STRING" id="36166.T1H035"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009501-RA">
        <property type="protein sequence ID" value="MESCA009501-PA"/>
        <property type="gene ID" value="MESCA009501"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029111_0_0_1"/>
    <dbReference type="OMA" id="QCRYLAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009263">
        <property type="entry name" value="SERTA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06031">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51053">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERTA" evidence="2">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009501-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13278" checksum="CA9532C06D81F89D" modified="2013-10-16" version="1">MTLEGNNNTCPSSNNSSSTTTQTTTTTSVDLDLDMFGPPRSTLPMGYYQRSRVPMISPKLRAREERKRILQLCATKLEGIKDSDTNLRRSVCINNTYSRLIDELRSEKQCRYLANLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0A4</accession>
    <name>T1H0A4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5'-nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02040087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0A4"/>
    <dbReference type="STRING" id="36166.T1H0A4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009577-RA">
        <property type="protein sequence ID" value="MESCA009577-PA"/>
        <property type="gene ID" value="MESCA009577"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006745_0_0_1"/>
    <dbReference type="OMA" id="RECNSAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF24">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="5">
        <location>
            <begin position="148"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009577-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="277" mass="30881" checksum="CE9D2D608E5D61CC" modified="2013-10-16" version="1">HNLDKKGIKIIIALTHCGYKLDKKIAKECPLVDLVVGGHSHTFLKSGKVDPIHPEHLNIRGPYPTIIVQKSGKQVPVVQAYCMSKYIGKLKLRFSKGDLIESNGDVIILNSIIPKDPEMLKMIEKYKSKVPKDEVLVRSRVKLSGWNECRVGECSIGNLLADAMAYARAKMLTKTNFPYATDASIAFLNSDGIRASIDKKSDGLIRQKDIRLVLPFKTKVFVVEMKGAGGILQMAGVKVTYNIKKPPGKRLGDDVQVLCANCEVPTYEPHIFHNYFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2F1</accession>
    <name>T1H2F1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2F1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010396-RA">
        <property type="protein sequence ID" value="MESCA010396-PA"/>
        <property type="gene ID" value="MESCA010396"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2765145_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010396-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8248" checksum="0C9A3DF6473CBB19" modified="2013-10-16" version="1">MLVRMDVMDVGVLDWQSCPHDGSWMLSIETTRYAGFPNRIYSHFSAYNIDYLGHMLVVVNWENMGYRDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2K6</accession>
    <name>T1H2K6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2K6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010455-RA">
        <property type="protein sequence ID" value="MESCA010455-PA"/>
        <property type="gene ID" value="MESCA010455"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="9894" checksum="7D8ED5734965033F" modified="2013-10-16" version="1">MASFCKDKIGKLITAKRKFLEGGNHSLPELFRIAARLGLFAIKIPITPFSSIFRKEDLRWLAGSSLSYCTSNLAPDYNSPVLETASLKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2P9</accession>
    <name>T1H2P9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U6 snRNA-associated Sm-like protein LSm6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA. Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome. Component of the heptameric LSM1-LSM7 complex, which consists of LSM1, LSM2, LSM3, LSM4, LSM5, LSM6 and LSM7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2P9"/>
    <dbReference type="STRING" id="36166.T1H2P9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010502-RA">
        <property type="protein sequence ID" value="MESCA010502-PA"/>
        <property type="gene ID" value="MESCA010502"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_7_4_1"/>
    <dbReference type="OMA" id="EQTVEYV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120115">
        <property type="term" value="C:Lsm2-8 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000010">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF1">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 16" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9 16" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="17">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010502-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8981" checksum="9B0DE67A24AB91C7" modified="2013-10-16" version="1">MSRKEALSQFINQIHGRPVVVKLNSGVDYRGVLACLDGYMNIALDQTEEYVNGQLKCKYGDAFIRGNNVLYISTQKRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3C7</accession>
    <name>T1H3C7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3C7"/>
    <dbReference type="STRING" id="36166.T1H3C7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010750-RA">
        <property type="protein sequence ID" value="MESCA010750-PA"/>
        <property type="gene ID" value="MESCA010750"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_991397_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010750-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="281" mass="31350" checksum="74653BAAB1FB8C64" modified="2013-10-16" version="1">MEEFSDYSSEVIGFNSTFQAEKSQGTSSACVGTDPLPPNVESSSQTTEKVSVDTQTKTEQLIFAKTDEVKLANWLKRILPQVEEELLQGLTPNPESSNIGDTGDNDILNVTVGQQIAFLGDNCSQGHALWLSLYSSDAPVLVLTASGHHENWCRHLNQWMKIFVPRRESGKDSLIFTEVKQLSLNACIQEISRNPFNKDMFAGVTVSGDLYIWLFKNDVKNVDVLEIANQSIQETPNSLDWLDETHLVGCLDHGQIIIWKVGQKMISQESTFTVQSKQKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4E8</accession>
    <name>T1H4E8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4E8"/>
    <dbReference type="STRING" id="36166.T1H4E8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011157-RA">
        <property type="protein sequence ID" value="MESCA011157-PA"/>
        <property type="gene ID" value="MESCA011157"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_944248_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF1">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="3">
        <location>
            <begin position="200"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011157-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="295" mass="33429" checksum="26DB9EE760B703C6" modified="2013-10-16" version="1">MNQWLGDDTANTYNSQNDDSRKFARTSTKTSLRIQSDLPIETPKSIRSKKQSKEVELSGAKSKKSLPKRSSNNNESDSEDTLSDEEHENSNLSGKKSLGSLKDNSDTNSRRSSLNKTVDISDSESIATSSEEEQQEKEDNFIDKEAPVEQVQTTYDTAYSTMTKSKVTVAEKVTRKHKTNSADNPKTKRKQKNPIKKSKSTKILAEIVKLQKDTKTMIPKLCFSRLVREVMQEIHHELYRVTPDCLHALQESAELYLTHFLSDAYLCTVHRGRLTLVPKDMQLALRLRGYNDPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4X5</accession>
    <name>T1H4X5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4X5"/>
    <dbReference type="STRING" id="36166.T1H4X5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011346-RA">
        <property type="protein sequence ID" value="MESCA011346-PA"/>
        <property type="gene ID" value="MESCA011346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_0_3_1"/>
    <dbReference type="OMA" id="ITASHAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000806">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24680" checksum="2D124965F9D1E95B" modified="2013-10-16" version="1">MLKNDHDSALDAGTIAGLNVLRIINEPTAAAIAYGLDKNITENRNVLIFDLGGGTFDVSILTIKGSVFEVKATAGDTHLGGEDFDTRLLKHLVEEFKRKNQKDLSSNPRALRRLRTAAERAKRSLSFSSEANIEIDALYEGIDFQSKISRAKFEEINADLFRETLDPVEKALKDAKMEKSQIDDVVLVGGSTRIPKVKNLIQNFFNGKTLNLSINPDEAIAYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5J4</accession>
    <name>T1H5J4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5J4"/>
    <dbReference type="STRING" id="36166.T1H5J4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011572-RA">
        <property type="protein sequence ID" value="MESCA011572-PA"/>
        <property type="gene ID" value="MESCA011572"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1528925_0_0_1"/>
    <dbReference type="OMA" id="NTSVIAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15545">
        <property type="entry name" value="PHD_BAZ2A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000199">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF2">
        <property type="entry name" value="TOUTATIS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="80"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011572-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="20251" checksum="F017BF66B9E2BDE8" modified="2013-10-16" version="1">MNPNSLKVTFKLRSPSSTRPKKTNSLQNCQFCTSGENEDKLLLCDGCDKGYHTYCFKPKMENIPDGDWYCYECVNKATSERKCIVCGGHRQPPVGKMVMCELCPRAYHSDCYIPPLLKVPRGKWYCHTCISKAPPPKKKTQKKSSSKDKDKDKDKEKDKDKDKDKEKDKDKESKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5V7</accession>
    <name>T1H5V7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5V7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012090-RA">
        <property type="protein sequence ID" value="MESCA012090-PA"/>
        <property type="gene ID" value="MESCA012090"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1063840_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047876">
        <property type="entry name" value="SHKBP1/KCTD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859:SF1">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859">
        <property type="entry name" value="SETA BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012090-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="262" mass="29227" checksum="31F91A5591779D7B" modified="2013-10-16" version="1">FTDMQKFPLRMKDNDLLVTELYKDPKDPITAISVYLTPKTSSLCGNWIEIAYGTKSGSVRVIVQHPETVGHGPQLFQTFTVHTSPVTKVIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H633</accession>
    <name>T1H633_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUS1 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="MESCA012166-RA">
        <property type="protein sequence ID" value="MESCA012166-PA"/>
        <property type="gene ID" value="MESCA012166"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015325_0_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF31">
        <property type="entry name" value="RUS FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="6">
        <location>
            <begin position="76"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="7">
        <location>
            <begin position="260"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012166-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="389" mass="44275" checksum="774058D0D43747B9" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLTTHAILKAIGVGSDYISPYSATITWIFKEGSGHIGKILFSWWKGSQLDVESKKWRLRADILNDIAMGIEIYLLPKYSELSTXXXXXXXXXXXXXXXXXGATRSALTQHHAIRGNLADVSSKDNAQETCINLFASFVGIYLLSVVENPSHLYANVKAVKSVVLRTFNESRYLIALEEYFRSGKVLTPYEVNKLERVTIGKTVSVSLNIQLGISIKYFVDEKNNFAVENYIATHDPHEKFIICECNKKFIGVFLHFEARQQDVLKAFFFAVSYLQDRSRYRFFEMSNKWLEFVNLAQQEGWLTTNHLLLVDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H740</accession>
    <name>T1H740_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SKI-interacting protein SKIP SNW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H740"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012522-RA">
        <property type="protein sequence ID" value="MESCA012522-PA"/>
        <property type="gene ID" value="MESCA012522"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006601_2_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012522-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="56397" checksum="864CBF67746E3D38" modified="2013-10-16" version="1">PLPINSKWDREDEEGSIADHQPIPGALVSANYSAPPYGKRKGWIPRTEADFGDGGAYPEIQVAQYPLGLGAPENVGKKSNALAIRLDESGKIKYDAIARQGHGKNKIVYSNINQLLPAEVLAEDAEELQRPDEETVNETTEATRKALEKLTNQKISAALPVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGDIFGLDQFLNMAKKAPKRNESSNDRSDKKRRRDRENNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H777</accession>
    <name>T1H777_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transmembrane protein 267</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H777"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012615-RA">
        <property type="protein sequence ID" value="MESCA012615-PA"/>
        <property type="gene ID" value="MESCA012615"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2055588_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026572">
        <property type="entry name" value="TMEM267"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13628">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13628:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012615-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13949" checksum="5DE532C45091BC87" modified="2013-10-16" version="1">VDNATHAVIGAITGIAFVIQFYDRISNCLGWLLILLCFLTSSFIDLDHFIQARSWNLEDATHLLRRPFLHCSTIPMVLFSIFVWTSCLNYFKTSLWIGVLFCAFFTHHTRDAIRRGYWLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7A4</accession>
    <name>T1H7A4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L27a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7A4"/>
    <dbReference type="STRING" id="36166.T1H7A4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012642-RA">
        <property type="protein sequence ID" value="MESCA012642-PA"/>
        <property type="gene ID" value="MESCA012642"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109163_2_0_1"/>
    <dbReference type="OMA" id="HSHGRIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="12"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012642-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9869" checksum="58F204772F4323BA" modified="2013-10-16" version="1">HLRRHHKFCPSINLHKLWSLGGNEKFEEISKDKSVKAPVIDLVQFGYYKLLGRGHLPKVHVIVYITYCQKAEDKIKKAGGVCLLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7K6</accession>
    <name>T1H7K6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BBSome-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7K6"/>
    <dbReference type="STRING" id="36166.T1H7K6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012743-RA">
        <property type="protein sequence ID" value="MESCA012743-PA"/>
        <property type="gene ID" value="MESCA012743"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3093470_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028233">
        <property type="entry name" value="BBIP10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14777">
        <property type="entry name" value="BBIP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012743-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="52" mass="6184" checksum="70ACD608CEB0FE4F" modified="2013-10-16" version="1">KMDLILPTTGKLFFETKTDLKFCQPQLMQLKSFTLEKLEIMQKEAHKQLQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA76</accession>
    <name>T1GA76_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GA76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000126-RA">
        <property type="protein sequence ID" value="MESCA000126-PA"/>
        <property type="gene ID" value="MESCA000126"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1457934_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000126-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="20538" checksum="E072B8FEFACB321B" modified="2013-10-16" version="1">AQSQIKGTDDVKKALDLLKESSFLKIENLLPYFSDFEXDDDRDNSDATPSEGHIESHQHHQSSSNSQSQMQGYYSSREPMMHHRGQGGGPPMYPPHQNQSGPGGPPNMGRPYMDNNHHQRLPQYQGGPPPPGGYPPYYRQPFMPQRPQGGYAMGPMGPGGPPQRQPGPQHNLAAVYQNVQQKLGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAJ0</accession>
    <name>T1GAJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAJ0"/>
    <dbReference type="STRING" id="36166.T1GAJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000247-RA">
        <property type="protein sequence ID" value="MESCA000247-PA"/>
        <property type="gene ID" value="MESCA000247"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019281_6_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000003">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF16">
        <property type="entry name" value="PAIRED BOX POX-MESO PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="8">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000247-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="250" mass="27572" checksum="EB5EF0A49B63854A" modified="2013-10-16" version="1">MRIVDLARLGIRPCDISRQLRVSHGCVSKILARYHETGSILPGAIGGSKPRVTTPKVVTYIRDLKHKDPGIFAWEIRDRLLAEGVCDKNNVPSTSSTGSGTNCSTGNHHSHIYNAIYNPYTYSMKAESCINPNPTHHIRTNGSQCWPSSHSVTDILANVHNQALRVTCNNNSCNGAPLPVVGQTHSGNLSISPTHQMIDEVANCSTPPHLNILNHQQQHHHHSHQQSYLYYNYFQNNSIHHGGITNATGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAN4</accession>
    <name>T1GAN4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4794 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAN4"/>
    <dbReference type="STRING" id="36166.T1GAN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000293-RA">
        <property type="protein sequence ID" value="MESCA000293-PA"/>
        <property type="gene ID" value="MESCA000293"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_597567_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588146" description="DUF4794 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000293-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="42647" checksum="F0FA0F7B51B8D0E9" modified="2013-10-16" version="1">MKCVLSVVAVLALVAVGAWAIPSGYMYYKPGTPGIMYVQYQKHGVGSQSDGVSAFVAGESGDTMSIVDDVKKGEDILSEEIAPEARTEDDSVTVDQGYSSQSVAIADDTPNNEYLPPVTDEKSALDEVLDEENVSLTNIEGDIQEAVADEDIPEETDDIIPVAIADSEITKEGSAESEEEEQNDEIKSEDEEKTGFEVYGVPKVEESEREPKNLEEDSLAEEVKTVDEEVKTVEEEVKTVVEENVEDSVEEPEAPVADDDSTVVVEEPTPVLKEIPEPEPVPVVPVVPAPTKRTRVFPARRPNTIPQRKKDEEEYVPVVRQAPRPKKEQSPLPLGGTFFPIDFGGANGGAIAIANSFSTGKGGHSQSHAVAYGNPRESQRSRKLAKKAGEDCDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB57</accession>
    <name>T1GB57_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000478-RA">
        <property type="protein sequence ID" value="MESCA000478-PA"/>
        <property type="gene ID" value="MESCA000478"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2747382_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000478-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8191" checksum="D630CA2FC35B842C" modified="2013-10-16" version="1">MDDNVATKKIYNASIEGVRRSGRSNLGLISNKIVFAVFFETRNLYQYLDPYLKKDYLNVATIIFLLVKINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBC5</accession>
    <name>T1GBC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GBC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000556-RA">
        <property type="protein sequence ID" value="MESCA000556-PA"/>
        <property type="gene ID" value="MESCA000556"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1356031_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000556-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22580" checksum="C514756DE662EF1A" modified="2013-10-16" version="1">MNYLTFVRNLLNFQNAPPQLLGRCLWTLSSYASSNLYNAQMLEEILGITLNSITTRENDIILKISAVRAIYGFLTTVSKGQEEKRVLILQSLPGFVDGILDLFQGCKPGVLGLLLEALTVIISFDMNFAASVCQKLIPLTIAVFLKYPEDPYILELVQEMLKALSQNPHCLKPLQEKIIPTLVSILNLQGEXPGKELVDFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBL7</accession>
    <name>T1GBL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA000651-RA">
        <property type="protein sequence ID" value="MESCA000651-PA"/>
        <property type="gene ID" value="MESCA000651"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654145_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000651-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="18215" checksum="CAA9FA9B0B8B8101" modified="2013-10-16" version="1">MFHEKLGDAYQNVKLRIHNITHDVIDFLVEIEVKIDNTVYCVESLSGEIHAIVANGERNLTTCIGIAYDDFKNVTIEIFNELDMVDRDYLDVKSLVEQCWGDKDNALLLMDCIIMGAAKIQTDIDTLIQNSKALGSMPXNSILTILMFVANMFYYQKCEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC38</accession>
    <name>T1GC38_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000834-RA">
        <property type="protein sequence ID" value="MESCA000834-PA"/>
        <property type="gene ID" value="MESCA000834"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1410299_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000834-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="21235" checksum="10AC0953E30D7CA6" modified="2013-10-16" version="1">MSMNLDDNGWATIPNMFQIIRLYGNQEPVNLISTSASKGNVSANYGANMSFVSEDTGTNIPSVQITRVQALPSYEFPQTSHNPRDPNQYGRQSAINEMTNQPIASPLILNHPPEHGNQQLFVGSPRFQDPRNLSSFGLSPSNFVEQLSIAAENCTPIGSACKDRNGALITNKKEVFMQFGIPSKFIKLSQMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCP0</accession>
    <name>T1GCP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02083791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCP0"/>
    <dbReference type="STRING" id="36166.T1GCP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001060-RA">
        <property type="protein sequence ID" value="MESCA001060-PA"/>
        <property type="gene ID" value="MESCA001060"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_819620_0_0_1"/>
    <dbReference type="OMA" id="ERTHCHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048699">
        <property type="term" value="P:generation of neurons"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002381">
        <property type="entry name" value="Putative spalt protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF87">
        <property type="entry name" value="HOMEOTIC PROTEIN SPALT-MAJOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="170"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001060-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="339" mass="37388" checksum="3B1F1DDD3435EF93" modified="2013-10-16" version="1">MALLPRPHSNDNSWENFIEVSNSSETMKLKELMKNKKLTDPNQCVVCDRVLSCKSALQMHYRTHTGERPFKCRICGRAFTTKGNLKTHMAVHKIRPPMRNFHQCPVCHKKYSNALVLQQHIRLHTGEPTDLTPEQISAAEIRDPPPSMMPGGFMNPFAFHFGGMGGHPGMPLNGNMGSESSHGDMDDNMDCSDDFDDDISDHMSNGGDSDRPKSGDDIRGMLFEQKLRIDNQRPRSRNSSCSGGAVSPRSSLHKASPGPSDDGALDLTPKAAPPPPQEHNVTTRAVTPPPPQPQPQPLVIPPPPPSSTSSASSLPSPSHQLVQCHHMVCHYCIIHHRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD38</accession>
    <name>T1GD38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02395094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02395095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02395096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD38"/>
    <dbReference type="STRING" id="36166.T1GD38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001217-RA">
        <property type="protein sequence ID" value="MESCA001217-PA"/>
        <property type="gene ID" value="MESCA001217"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1078857_0_0_1"/>
    <dbReference type="OMA" id="PEERTHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="50"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001217-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="258" mass="29333" checksum="1775F1C7585A2EF9" modified="2013-10-16" version="1">MIEVSFKEAAAKDFWCERPSHLKDVPVDVWRKISNTSDNCDVAKIDWSQISLADALTITINSSDKSVAREYHIPEERTHCTKWEFDRNDNIGNNWISEWQLVCESGYLTSVAEMFFLVGVATGGILSGYLSDKFGRKRLLFISTMMQTICALALYFITKFELYLALRTCLGFASVSVTYAALILAIEYVDGDWKTIAGMYNLFPLPLSYAMISGIAYLTQDFQRLQLCISIPGIFLLLLWFVVPESPRWLLVKGKIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDJ7</accession>
    <name>T1GDJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gem-associated protein 5 second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02027395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDJ7"/>
    <dbReference type="STRING" id="36166.T1GDJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001385-RA">
        <property type="protein sequence ID" value="MESCA001385-PA"/>
        <property type="gene ID" value="MESCA001385"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_779117_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056424">
        <property type="entry name" value="Beta-prop_GEMI5_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052640">
        <property type="entry name" value="Gemin-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362:SF1">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23775">
        <property type="entry name" value="Beta-prop_RIG_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gem-associated protein 5 second beta-propeller" evidence="1">
        <location>
            <begin position="215"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001385-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="356" mass="40175" checksum="2FD0837570440A1B" modified="2013-10-16" version="1">MQSWSDVLVLCVAFDSPDDIVMSPLSNDEVTLQDCQNSQTVNKNTSLSCGSSHSMELISSVEESANEEHVLVHNAEVHIPKVEENKDKNIILATASKEGKICVWNTNNGRSLVNYRLKNNSLDQKQYLNFKFKIWELLLFTLVSKDKDQFALRDERKKFHSKNGVVALCLLKNNPEIVWTLSYNRELICENTKSGKLLAKFSCAATNISCLKERPDDMNKIAFPCNDKRVSILNISTMTNTNITIDNHTNKGFYCIRALAWSPVDQLLAFGTSEGRVGIIDTEKVHPISGKNKESYQGPFIMNPFFGKPVYSMAWSGNYIYICCNDFVIAYCGRRKSKASPDLYGYDQGLKMVPDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDR3</accession>
    <name>T1GDR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Armadillo repeat-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02028778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDR3"/>
    <dbReference type="STRING" id="36166.T1GDR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001458-RA">
        <property type="protein sequence ID" value="MESCA001458-PA"/>
        <property type="gene ID" value="MESCA001458"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039447_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22895">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22895:SF0">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001458-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="43716" checksum="49ED2F68379F9D9A" modified="2013-10-16" version="1">VFAAKLGANNILVTIVETQIVGDSPEKNPEIISASLKSFNALLNKQPDLFDAESLSLVIKLIQEFKENTQILSLTLQWLAKSCIMHENNRQNIIGADIMTYLKPLVGIKNDVIIRDLCACFRVEFGKAHEHARIIASSVLVELTQLLPEYEDPLLLSDMILTIASLAVRQELCVAVEDANGLEYVFDVMKKHPDHPKLNKEALKLLRALAGNDTTKVHIVQKGAAPIVQNILVIHKENEQVAGTALACISTLTLRVKDNSTAFFETGVAENIADVMTLHPKSKVVQRNAAWAIRNMVSRSRDQCDSFLSHGVEDLLNQAMKDHPSVIQDVKAALRDLGCKVHLKEEWTGTAEKAKIISNPYRGSYQKSYARKQIDDESDVRFLHSEQYSNAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEB8</accession>
    <name>T1GEB8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEB8"/>
    <dbReference type="STRING" id="36166.T1GEB8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001677-RA">
        <property type="protein sequence ID" value="MESCA001677-PA"/>
        <property type="gene ID" value="MESCA001677"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1356030_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001380">
        <property type="entry name" value="Peripheral-type benzodiazepine receptor-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040325">
        <property type="entry name" value="RIMBP1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234">
        <property type="entry name" value="RIM BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234:SF19">
        <property type="entry name" value="RIM-BINDING PROTEIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001677-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23141" checksum="3D645E82D222DB05" modified="2013-10-16" version="1">MYIISIPAPKQLTLERQLNKSVLISWTSGEPVGYNTIESYHVYVDGVLKVTVKANEHTRALIEGVDSTRPHRISVRSVLHNRQTSRDAACTMIIGRDTSHLGPSAVRASHITCSQPLFRGYQPIQITNMLFVSTMWKCEQLNQELIAIQLRVYHHLLNTVSRSGQNTYALEMLPFLEDLWKKLQEHMLTLGLFLKDYLIRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GED3</accession>
    <name>T1GED3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02054791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GED3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001694-RA">
        <property type="protein sequence ID" value="MESCA001694-PA"/>
        <property type="gene ID" value="MESCA001694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530037_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9504" checksum="8DBE9D62C8432DE2" modified="2013-10-16" version="1">MPMSTIYKEKAKKALVKDSKTPVYIKKSDGSCTESIDEMGIILIYTHFPDSEEDLVVKDSSHGLNPLVPSWMDAMERIITSKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEI8</accession>
    <name>T1GEI8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02083978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001752-RA">
        <property type="protein sequence ID" value="MESCA001752-PA"/>
        <property type="gene ID" value="MESCA001752"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123888_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001752-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12918" checksum="E2D2EE748344BFC5" modified="2013-10-16" version="1">MLSSRNQSHQKHWAQSSPLKIMTSRRSNFCLHYWSKLFLLVALALFFSHARRFTDILCHIASSALSLLINAVLSTMKETDYEQSSNILDTKGIVSIFGYLSTRKVKSGTSFTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFC7</accession>
    <name>T1GFC7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02054010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002065-RA">
        <property type="protein sequence ID" value="MESCA002065-PA"/>
        <property type="gene ID" value="MESCA002065"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199957_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588246" evidence="1">
        <location>
            <begin position="21"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002065-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12539" checksum="02473FD37B7D1337" modified="2013-10-16" version="1">MKGGIFFGFFGILLINQCNCLKKDQKYNNLAGVYFNNVGTYNFVDNKDETFLTSFTLTGLENTLSNAKTYKYESLTLEENLNNIYKVCNRNRKNYLPYESTMIPENQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFE8</accession>
    <name>T1GFE8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFE8"/>
    <dbReference type="STRING" id="36166.T1GFE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002089-RA">
        <property type="protein sequence ID" value="MESCA002089-PA"/>
        <property type="gene ID" value="MESCA002089"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2335995_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002089-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11687" checksum="CE5960B05779604F" modified="2013-10-16" version="1">MLYKDEGNSCFKSGKYRAAIKFYEKAIEKCPEDNNTDMAICYQNRSAAYEMLKRCNQVIADCTKSLQFRKYMKSYFRRTKAYEQTKDLLKFWNDIIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFH9</accession>
    <name>T1GFH9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFH9"/>
    <dbReference type="STRING" id="36166.T1GFH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002121-RA">
        <property type="protein sequence ID" value="MESCA002121-PA"/>
        <property type="gene ID" value="MESCA002121"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075165_3_1_1"/>
    <dbReference type="OMA" id="VEYGHYP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF95">
        <property type="entry name" value="CUTICULAR PROTEIN 76BD, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588251" evidence="3">
        <location>
            <begin position="17"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002121-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="14904" checksum="3816CE3B673A36B9" modified="2013-10-16" version="1">FLVAITIALVASVAYSIPIDVGHYAAPAYAVHAPVVHAPIIHAPVHKVVAEPVAYPKYSFNYGIKDPHTGDIKSQAEERDGDVVKGQYSLVEPDGSVRTVDYTADDHNGFNAIVHKSAPKIVAHPAPVIAHAPLYHGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFL2</accession>
    <name>T1GFL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidylinositol 3-kinase catalytic subunit type 3</fullName>
            <ecNumber evidence="1">2.7.1.137</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphoinositide-3-kinase class 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02189034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFL2"/>
    <dbReference type="STRING" id="36166.T1GFL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002156-RA">
        <property type="protein sequence ID" value="MESCA002156-PA"/>
        <property type="gene ID" value="MESCA002156"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002191_1_2_1"/>
    <dbReference type="OMA" id="PYGCLCT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000008">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF118">
        <property type="entry name" value="PI-3 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="11">
        <location>
            <begin position="25"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="465"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002156-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="626" mass="72414" checksum="43C3F7D2450BB592" modified="2013-10-16" version="1">ENSQKSIENKIEEPLKEKYISSWSINAEFKLTLHSIYDLNCDLSKYTDICVYLGLFHADKLLCSQKESSEKPIKNRESVKYEENIIFPIKLGDLPRMTKLCIVISERNKSIKLSKSKKINNKITGRPLAWTNATIFDYKGFLKSKELTLTTWTFADDNQYDDIFNPLGTVEANPRSDECATIKLTLDQYGFENNIKYPSEAEILKYSATVAKENLCKDRDEGSAQDTRSINVVLTPYSKSEKIEEMVDQDRNVIWSKRLDVLNGAHEELPILLRCVNWNKKEEVAEMISLLKNWKLVSTERALELLDYSYPDPSIRKFAISSLKQLEDEELLLYLLQLAQSIKHEAYLNCDLVIFLIERALKNQRIGHFFFWHLRSEMHVPCIQNKFGLILEAFLKGTHISQINAIQKQLEGFEKLKEGSELAKKGNKEKVKAVLQEFLGRRKFNDIFNKFQNPLNPSYSCKGIKVDCCKVMDSKMKPLWIVFENLISSNDDISIIFKNGDDLRQDMLTLQMLKVMDKIWKNEGYDFRMNIYDCISMEKKMGMIEVVLNAETIANIQKKSRIFSATSPFKKGSIFAWIKEHNQTEDQLKKAIEEFTYSCAGYCVATYVLGVADRHSDNIMIKKNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFP1</accession>
    <name>T1GFP1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-carbonic anhydrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFP1"/>
    <dbReference type="STRING" id="36166.T1GFP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002188-RA">
        <property type="protein sequence ID" value="MESCA002188-PA"/>
        <property type="gene ID" value="MESCA002188"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114674_0_0_1"/>
    <dbReference type="OMA" id="FQTANEG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00326">
        <property type="entry name" value="alpha_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF137">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16038" checksum="9F3866075DA28A4B" modified="2013-10-16" version="1">MGFFFTLGEAESNGLQTLVRHLPMVPNSGDQLSMNVTFSLTSLLGNIDTDKFFTYKGSLTTPPCSEAVTWVLFPDPIELNYLQIQKFRRLQDNLEDALVDNFRSIQPIGNRRVFYRKVNSFATDKALIENSVHHSEVDWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG13</accession>
    <name>T1GG13_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02105813">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG13"/>
    <dbReference type="STRING" id="36166.T1GG13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002317-RA">
        <property type="protein sequence ID" value="MESCA002317-PA"/>
        <property type="gene ID" value="MESCA002317"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_824618_0_0_1"/>
    <dbReference type="OMA" id="FTEFECL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF263">
        <property type="entry name" value="PICKPOCKET 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="337" mass="38024" checksum="7A18D0BAB01A1C6C" modified="2013-10-16" version="1">MVILLNKKQWVPPKGTAYQKADEFLEKNGITIRQAAFSVSSNCSDFLRTCYWGFTEFECLDFSSDNILKFSLSTTYLGPCCSFNANSVNNSFVPFSTNSFGLNSGLTVVGFEGRYSNLSTGLIILIHHPLDYPTEAVKTVTEEYGIISGNQSRTFSTSAEILDLTPDKRNCYTSSDLEFNFYRASICTLTCQNEAVFEKCGCYPYHMWTSGAKAEKECKLKDSFCYSENFGKLKNTKCEDCLPNCNDVTYDSSAYNIDLLSSHFSIHPLSLFSSKDTVLTSQGLIVRIFLPKQVISSYKKLVVTTWMNLLEQPLNSTIHLSRRTELPSESRNRIDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG44</accession>
    <name>T1GG44_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG44"/>
    <dbReference type="STRING" id="36166.T1GG44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002353-RA">
        <property type="protein sequence ID" value="MESCA002353-PA"/>
        <property type="gene ID" value="MESCA002353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109340_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22421">
        <property type="entry name" value="KH-I_BICC1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047554">
        <property type="entry name" value="BICC1_KH-I_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF69">
        <property type="entry name" value="PROTEIN BICAUDAL C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="143"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="251" mass="27102" checksum="C0C0AF606E6853AE" modified="2013-10-16" version="1">MENFRRIIDSNSTRGQYNMLASLGFNRLTYGSTGAGSGPGTGSVTGSGAGSGAGAGTPATSSGPPSETQSEISSVDSDWSDIRAIANKLGVQNPDDLHTERFKVDRQKLENMIKNPHVRIVGKPEDVQKAKECVLASLDSRGTRVIMKMDVSYTDHSYIIGRGGNNIKRIMESTKTHIHFPDSNRSNPTEKSNQVSLCGSMEGVEKARSLVRLSTPLLISFELPVLAPGRPAPDHDTPFITNVENQYNVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGC3</accession>
    <name>T1GGC3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002437-RA">
        <property type="protein sequence ID" value="MESCA002437-PA"/>
        <property type="gene ID" value="MESCA002437"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103919_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577348" description="Protein TsetseEP domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002437-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="28560" checksum="A9CE4E75E9A3DE37" modified="2013-10-16" version="1">MNTLFYFTVILGVIALTGITTGAYNDPMPNNLYYLVNHTISENNNRILFHEIQLNLTQNRFDHYVETVRHNNKMVERKISEFTSELTLFGFLNETTQNCVVKYGKIPDVEAFKLKINTCANTAGSSFNSYTRILQVAFNNLANAKSYNSYIRSESQKCYNDYIQYVNKMANCINTSIERRNSNIDVSLINIQNELVKALNTYDVISRDTSECIYKIEFELGKLFDNQWNVLRTCQKIGKKSTFFGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH62</accession>
    <name>T1GH62_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH62"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002748-RA">
        <property type="protein sequence ID" value="MESCA002748-PA"/>
        <property type="gene ID" value="MESCA002748"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388713_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002748-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10268" checksum="D5906B5139E8BC36" modified="2013-10-16" version="1">MRRATKKGSATPGGESLNMLGRSVKSPMNDEVSAEQTSSTEAEPEPEPEETKASQEEEEEDAPSEDVASEQDDEENKTLETIEIADLSLQCMHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHG6</accession>
    <name>T1GHG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02083758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA002865-RA">
        <property type="protein sequence ID" value="MESCA002865-PA"/>
        <property type="gene ID" value="MESCA002865"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559152_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002865-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="19510" checksum="6586D621322FE5F1" modified="2013-10-16" version="1">TQIYQQPTFQQPQNQTQSNFQPQAQIIHHPAAGAPQQQQQQVLQQPVLNQHLQAQQQNVQQIQQAVIPNQSSQIQHQITQPQQQTPQPIAQQQQQQLQATSQQQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHN7</accession>
    <name>T1GHN7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002945-RA">
        <property type="protein sequence ID" value="MESCA002945-PA"/>
        <property type="gene ID" value="MESCA002945"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2374950_0_0_1"/>
    <dbReference type="OMA" id="FPIRISW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002945-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10706" checksum="3D2BCEA2F4F6C8C4" modified="2013-10-16" version="1">MQRCPYIHEMRERLLGDNNRETLQLESMERDNLIDNRHSSETNQLHADGYHTSPAHQRTPLVPRDLGEDFNLDFDDDFNLDFRRPKNAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHN9</accession>
    <name>T1GHN9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02059769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002947-RA">
        <property type="protein sequence ID" value="MESCA002947-PA"/>
        <property type="gene ID" value="MESCA002947"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429574_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002947-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10950" checksum="FD938E12476A6098" modified="2013-10-16" version="1">MQIRQKKLRYENEEIQKLEDLRESNQTRKFYQVAIILPQCRKSSTQAKLSTIQRASDWSYVLSFRKGKGEHGSWEEWKDKERYTLFSSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHZ8</accession>
    <name>T1GHZ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142282">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHZ8"/>
    <dbReference type="STRING" id="36166.T1GHZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003064-RA">
        <property type="protein sequence ID" value="MESCA003064-PA"/>
        <property type="gene ID" value="MESCA003064"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362118_0_0_1"/>
    <dbReference type="OMA" id="ERADNYK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="1">
        <location>
            <begin position="20"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11088" checksum="9A1F2379777041E6" modified="2013-10-16" version="1">MNMKMPDFNVKKLVKEAGTQLSRVVQLTEEKLGTSERTELDSHFENLADRADCTKNWTEKMVKNTEAVLIPNPANRVEDFIFTKIEKDKPARLSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GID9</accession>
    <name>T1GID9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GID9"/>
    <dbReference type="STRING" id="36166.T1GID9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003213-RA">
        <property type="protein sequence ID" value="MESCA003213-PA"/>
        <property type="gene ID" value="MESCA003213"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1181393_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003213-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="235" mass="26670" checksum="D6125C1A78F6BF00" modified="2013-10-16" version="1">MQDSKLFEFIRLDDCDKVRELLMKDFSPDLQWDDEFTSSFRRKSIGSDDEWSKLFQYYAKTFETTGDILASTEPKKARKPREPHTVDMNGKSPLHYAAVLEDSTCLGILLGIRAEVNGVTKEGYTALHYAVNNPENVRILLDFNANPNKCSFVLMDTPLHMAAAVGSLNVNNFGCIGVDPISLQSNSHIYYPRFCSAQRWADIPRANMCSSLMSQFSFKGYSRIYYNIVSQAPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJA9</accession>
    <name>T1GJA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003554-RA">
        <property type="protein sequence ID" value="MESCA003554-PA"/>
        <property type="gene ID" value="MESCA003554"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608769_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003554-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9477" checksum="D675981AFCAF2E2F" modified="2013-10-16" version="1">MERKKFPYMLVCVCDSNLYSLCTQYLSNKLSASRGDSFSDLPKKLKWENQSWWNREIVVNVRNSQLPQTMNIYWKHTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJC1</accession>
    <name>T1GJC1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJC1"/>
    <dbReference type="STRING" id="36166.T1GJC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003566-RA">
        <property type="protein sequence ID" value="MESCA003566-PA"/>
        <property type="gene ID" value="MESCA003566"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_447113_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000048">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000068">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="238"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="321"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003566-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="611" mass="68254" checksum="C6AFECF4FCF581FD" modified="2013-10-16" version="1">MMPSYVDHLTYLQHAWAHGPPPAPTPPSDFHFSLSGSSNSSRARKRTLSSSPYSDSFDINSMIRFSPNSLAGARTTTNGNYGHLSAGGIPHLLGLIHPTHSSAFIGHNYMETDVPIPAQKKVAVARAEADSSLSTPKKKTGVRRETEQAQKVSTTTPDAEVQKDEPGDFIETNCHWKNCGVEYLTQEELVRHINNDHIQTNKKNFICRWENCSREEKPFKAQYMLVVHMRRHTGEKPHKCTFEKCCKAYSRLENLKTHLRSHTGEKPYTCEYPGCSKAFSNASVEQNIKIKPYICKAPGCTKRYTDPSSLRKHVKTVHGADFYEKKKHKGNNGNNNGGTGKSIFMGSSGSSDYPKSFNNLSPSIKSESDSPSIPSAQQDTVAAADDPLWPYDDEDLEVAELPGVLLLRGNSRLRPRFPGKSHLLQGVQQIQSNDFFTDIKSDRRDSEQSNASTYYCSMRSESGSRRVSTANDSFYDPISPGCSRRSSLVAPTPSSMHFPGVFGSRRLSEPIKIDHHPNEEIVLDEVGEEEMVENKIVVPDEMLLFLQQDGREIQVADISQSTVSYQRTLEYVQDCQDFLAADSSTHPNLVVGDLESSLNSSWKKINFYNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJK0</accession>
    <name>T1GJK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003649-RA">
        <property type="protein sequence ID" value="MESCA003649-PA"/>
        <property type="gene ID" value="MESCA003649"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3242720_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003649-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="43" mass="5065" checksum="306EAC45C61CFE39" modified="2013-10-16" version="1">MTLKNILFLPNVLWSNNSRFLAINFIMNSFNNTSFINIKVNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJW0</accession>
    <name>T1GJW0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003767-RA">
        <property type="protein sequence ID" value="MESCA003767-PA"/>
        <property type="gene ID" value="MESCA003767"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963416_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577430" evidence="1">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003767-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6452" checksum="6E30E66B9BBD5D9C" modified="2013-10-16" version="1">MRAMMPHQGPGPTMESIFMALLCVACASNYIRPGPATTTLPTPDNQLPCIENIKFCKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKH8</accession>
    <name>T1GKH8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKH8"/>
    <dbReference type="STRING" id="36166.T1GKH8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004002-RA">
        <property type="protein sequence ID" value="MESCA004002-PA"/>
        <property type="gene ID" value="MESCA004002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161251_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12658" checksum="613E8F69C6139BC9" modified="2013-10-16" version="1">MRKGYRHEDSPWQSSRRRDGHLLSSFRLWAEVFHVSSGGGGAVKWQQVSEDLVPVNITCIQDSPECVFHITAYNSQVDKILDVRLVQPGKFDIIFLYKASQVSRFALCVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKT7</accession>
    <name>T1GKT7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKT7"/>
    <dbReference type="STRING" id="36166.T1GKT7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004117-RA">
        <property type="protein sequence ID" value="MESCA004117-PA"/>
        <property type="gene ID" value="MESCA004117"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815369_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588365" evidence="1">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004117-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7829" checksum="BC7482A47EEC91CB" modified="2013-10-16" version="1">MMCVYLNIVSLLYYLVKCEHLVCRRKISLSPSLAVDCKLFTLDSINTFRKYISLLDLILTCLLSTHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKX4</accession>
    <name>T1GKX4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02138160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKX4"/>
    <dbReference type="STRING" id="36166.T1GKX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004157-RA">
        <property type="protein sequence ID" value="MESCA004157-PA"/>
        <property type="gene ID" value="MESCA004157"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_667803_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF0">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Serine-threonine/tyrosine-protein kinase catalytic" evidence="4">
        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup54 alpha-helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004157-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="45588" checksum="283708F4301945BA" modified="2013-10-16" version="1">MYSSNPNDYELRVELANCCSDIATVYLSQYLPTEEHVAVKKYRLDSASNTLFNQIQHEIITMRQLNHPNILSFHIAYVNYMEMYLISPFMCYCSCSDTMKNIFDRAFGATQTNTAFGGFGSAPASTAAPAFGGFGATATTTAAPTFGGFGAATATSSSTGFGGFGSTFGSTFGKPAATAAPSFGGFGSFGSTLGQPRKHNHQLLEISPDEAFAQSIFNVSIFNDERDATIAKWNFLQASWGTGKSFYSQAAVPVEITPDNYLCRFKSMGYSRLPGKDNKLGLVALKINKPQSEVEAQKQQIITTLGSIFGSKPNLQVNIDSIKSLEDKKTQIVIYIEEKSLQIPNDIKRFNELKWRIKCQEQETEAHSLYLRKLEKDLVQMKQKHVATTAKIMERKRKLAELTHNILKVCDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLM1</accession>
    <name>T1GLM1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004431-RA">
        <property type="protein sequence ID" value="MESCA004431-PA"/>
        <property type="gene ID" value="MESCA004431"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1742633_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004431-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="16780" checksum="8CB7DF1E5B6BB7C2" modified="2013-10-16" version="1">MNMTILTERMTSGSSLQHPRNSYRPWVGKYRGQNCSSPTECPVLQESVQYSLMGQSHQKSSGVFITEFDYKDAISLEDCSLFHRKLVAKRHDSAFSVFQAALMSSKCWVPGHVEIEVNEIADELPRKGSVAPGGKQGVLNTDECIQEPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM56</accession>
    <name>T1GM56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02025913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM56"/>
    <dbReference type="STRING" id="36166.T1GM56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004624-RA">
        <property type="protein sequence ID" value="MESCA004624-PA"/>
        <property type="gene ID" value="MESCA004624"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827531_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000061">
        <property type="entry name" value="Ets DNA-binding protein pokkuri"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNT" evidence="1">
        <location>
            <begin position="49"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004624-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16212" checksum="51922A5473788E97" modified="2013-10-16" version="1">MQLDQNVDYNTNFNNRVPPHSSYIMDSSSWDSISIMDLNSSSPILTKINSLVSNSFDEYNDHLPADPRNWSRADVWKWLETTATNEGLPDVITHELPMKFPMNGKALCLMTLDMYLSRVPVGGKMLYRDFRTRLARAMSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN93</accession>
    <name>T1GN93_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Myosin N-terminal SH3-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02140466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN93"/>
    <dbReference type="STRING" id="36166.T1GN93"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005037-RA">
        <property type="protein sequence ID" value="MESCA005037-PA"/>
        <property type="gene ID" value="MESCA005037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1526923_0_0_1"/>
    <dbReference type="OMA" id="VKSHQIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003012">
        <property type="term" value="P:muscle system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="10">
        <location>
            <begin position="33"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="134"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="20557" checksum="BF5B95B6E069B697" modified="2013-10-16" version="1">MPRPIPNQEDEDPTPYLFVSLEQRRIDQSKPYDSKKNCWVPDEKEGYLLGEIKATKGDIVSVGLPGGEVKEVKSHQIMQVNPPKFEKIEDMGDMTILNEATVLHNLRQRYYAKLIYSKDFKKDQLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYHKLIYLSVSYLITFTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNB9</accession>
    <name>T1GNB9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNB9"/>
    <dbReference type="STRING" id="36166.T1GNB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005066-RA">
        <property type="protein sequence ID" value="MESCA005066-PA"/>
        <property type="gene ID" value="MESCA005066"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1075821_0_0_1"/>
    <dbReference type="OMA" id="YFERSTY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.950.10:FF:000001">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF9">
        <property type="entry name" value="UNCOORDINATED 115A, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HP" evidence="2">
        <location>
            <begin position="247"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005066-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="312" mass="35758" checksum="693AF2C81D5650C0" modified="2013-10-16" version="1">MRVLVDTIRSETPRPKSPGMNNEEPIELSHYPDAKKPLPGEKPKIERDDFPAPPYPYTDPERRRKYSDSYKGVPASDDEDEVDQENIGVTEEIYESPIGASPSRNLDHQKPFYEDEYFERSTYYRGSIGKSIGNATSYNAIHSYRSPPKPGYGFKVSTLPPSSIRTNGYCSDYSFGGMGDKTHSTDFSCNKSEASVDSITEGDRRALMGCDLPPSSTYSGIPLSYHYPQAGLMRRSLPNMAHSMLVHEPAKIYPYHLLIITNYRLPSDVDRCNLERHLSDIEFENILQCTRAEFYRMPQWRRNEIKRRARLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNE0</accession>
    <name>T1GNE0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02181691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02181692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNE0"/>
    <dbReference type="STRING" id="36166.T1GNE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005088-RA">
        <property type="protein sequence ID" value="MESCA005088-PA"/>
        <property type="gene ID" value="MESCA005088"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1564653_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000019">
        <property type="entry name" value="Myc proto-oncogene protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050433">
        <property type="entry name" value="Myc_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002418">
        <property type="entry name" value="Tscrpt_reg_Myc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45851">
        <property type="entry name" value="MYC PROTO-ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00044">
        <property type="entry name" value="LEUZIPPRMYC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="3">
        <location>
            <begin position="85"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005088-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="19572" checksum="ED852375CBE5B354" modified="2013-10-16" version="1">MSGQRKRVNNSAGHIIVNQNVRDDYLPPTAKRHRLSNGCGSNSSSKITKKMSSMSSASSTTSSCSGSSSPTKQRHFSIDDIDSNEKRNLHNDMERQRRIGLKNLFEALKKQIPSIKDKERAPKVNILREAAKLCDQLTSEERDILRQKHLLKEEIRQRQDRLRRLKSMSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNR1</accession>
    <name>T1GNR1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNR1"/>
    <dbReference type="STRING" id="36166.T1GNR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005220-RA">
        <property type="protein sequence ID" value="MESCA005220-PA"/>
        <property type="gene ID" value="MESCA005220"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925262_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005220-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6843" checksum="280425CBBEE917FE" modified="2013-10-16" version="1">MNKCSAKFVNKLKLSTKDNQIIDIQEWVHKCTLDIICETAFGSEINSLDNENGEIVSAIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP56</accession>
    <name>T1GP56_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP56"/>
    <dbReference type="STRING" id="36166.T1GP56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005373-RA">
        <property type="protein sequence ID" value="MESCA005373-PA"/>
        <property type="gene ID" value="MESCA005373"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507443_0_0_1"/>
    <dbReference type="OMA" id="NICDANA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005373-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="537" mass="59453" checksum="62FAED8764DD90E3" modified="2013-10-16" version="1">MEEKCHEKGILKKHGYTLALGDQIKDKWGDDGQSDNMELMAQQDVSGGVGIGVGGAAASEVERTLKSLNGYHEDILEALRNAASHRGTGAGNTPVGSNSFTNNLNYGEYKRGGSTKSSRENICDANAAHAILIEGVGGLGSSGGMIHQHHHRSQHHLHQAAQQQQQQQQQAPLQQGEDDDAPATGPLRIRNLEDLIRQLEQHSSRHMSPSGSEDIRMSETEADRHYRLDSSAACSESSQGSTQQLAQSQKTATIHPPYGRCRPRPDDENNRFTSPAAVAERHQSTTSTSVAFAPCLRPPYSSFPFPWPLDAWSTFIHHSSHTHLHQDDDAMYESAEHHTLDPRETPDSESDDFIKAQQQLARWASEDVVSVVVLDPGSMSDSQQQLSSLHHHHQSQQPISVQQQQQQTMQQQHQQQQQQLQNTIGHTQQCTAVQSGHILQHHGGDTVSVTTGITQNNMQQQQQQQMQQIQVTTNGINPVQQRDYYPSPPSTTETESSGSVIPPTIQQRRSIILDSNQIQMVGDGQLMENGRYPEYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP95</accession>
    <name>T1GP95_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005416-RA">
        <property type="protein sequence ID" value="MESCA005416-PA"/>
        <property type="gene ID" value="MESCA005416"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415784_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005416-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="11106" checksum="074CAA549B59C211" modified="2013-10-16" version="1">MRGEDPNAKKQQNHRSADALDDDFYFGGDDEEEQNILLMVSMDKEKLLRKLKKRKVAIWVKIWMVKMMRMLKEKMTPKEKKKMVKKFMLKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPM6</accession>
    <name>T1GPM6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005560-RA">
        <property type="protein sequence ID" value="MESCA005560-PA"/>
        <property type="gene ID" value="MESCA005560"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714318_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577069" evidence="1">
        <location>
            <begin position="24"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005560-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="387" mass="43524" checksum="552C993400D66CCF" modified="2013-10-16" version="1">MNFKTSFVLLIIGLSLAPLTVKGECTEDENDGEFKKFFKKVSCNIQEGAEKIGEKSKPYLDSIRNETSKLADKAKPYLDSIENGAAKLADNAKQLGSDTVDKFNEWRGKVNEANNEPVQMNGELRTAAEAPKPSVDFAFPDESVTVKIDDRFLLDGGSHCKPGERDTGTGTPRCRKIIDISVKVKKGEKRRPQFLEKYWDPGNNTRYSRLHQQHSCSNSLRNHPIATTADIQLQSLNAQYLLNSISTANETNIGVNSSCSKASSSLDLEWENEYGHDNHALHHSWLFFPTPKSGPTVNSSNISTENVSRNSWSHISTPESLEWDVDEEQRDKLFTEDDNLDHETLQLLHEIEFLKNQVLSETGFDCLGITTYIDNNNENELDDNYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPR3</accession>
    <name>T1GPR3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPR3"/>
    <dbReference type="STRING" id="36166.T1GPR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005604-RA">
        <property type="protein sequence ID" value="MESCA005604-PA"/>
        <property type="gene ID" value="MESCA005604"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_421093_0_0_1"/>
    <dbReference type="OMA" id="NIERNAH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17936">
        <property type="entry name" value="EEXXEc_NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZNFX1" evidence="3">
        <location>
            <begin position="194"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="2">
        <location>
            <begin position="372"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="500"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005604-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="651" mass="74967" checksum="7FFF9924E9634CA7" modified="2013-10-16" version="1">MLGILARVCDTPLEQYKHEYLSTFVDSSEFLDGVRSLLKKIFMDTKNKSKKSIGYQISPLELSSKLMTIFKSIFSMNLWNDTIQNLFSVLSERLANSGSKFADLKIEVEKVSSLLERQAHRNLPEDIYPTLEELTTNTELDLKPNIVNGDYVNVENYVDIHANLLREDFLIPLRDLITELKSHENLDSISSNKIYKNNFVERSNNTKNKKGELVLVDFKAGERNESPNHIGVESTWVNPKKLMYGSLLVFSKDLKFESVILAVVSNREIESIQKGYIQIEIVKMYNIGKVFGEEFIMFESDVYFEPYHYTYKVLRSLNEDNFPLANYIVNIERNAHPPTYLESSKLQTYRYNKSCFYPLDHSTYSSINETLKLDSSQSKAYFHALSNKFSLIQGPPGTGKTFMGLKLASTILKNIQDCQVAVICYTNHALDQFLVGLLPLLEECNLSEDCIVRMGNQSKNELLDKYNVKQISDATTITDRRLKACFYQTKNDYMKTFQEFEKLQQEYAAVMEDIIKVQRTLRKISRKLDELKQISDFNTIKDKRVIAMTTTYASRQNILLQLLATPIVIIEEAAEVLESHIVASLNKNVQHLIMIGDHMQLRPTTSVYKLSQKFNMNISLFERMTRGNGRNSPTKSFEKTNSLIVQPRTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQL9</accession>
    <name>T1GQL9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005929-RA">
        <property type="protein sequence ID" value="MESCA005929-PA"/>
        <property type="gene ID" value="MESCA005929"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1557040_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577561" evidence="2">
        <location>
            <begin position="18"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005929-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="18390" checksum="304896C4496EF681" modified="2013-10-16" version="1">MKVILVLALVYVFGAECQIFSQTPAPSSVFGPVPGGRPTAEEIQRQREEAASKTSTIRTASASISSGPVSSHAAISNSLSTPIVRSSHAAISNTFTAPNIRTAISNSFPAPAPIQVPITKTFSPVPQQSAHTLTNNGYQYKSVRRFRVSRTINKYLRKSCKAICLFLGLGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQR0</accession>
    <name>T1GQR0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQR0"/>
    <dbReference type="STRING" id="36166.T1GQR0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005973-RA">
        <property type="protein sequence ID" value="MESCA005973-PA"/>
        <property type="gene ID" value="MESCA005973"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_1_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09993">
        <property type="entry name" value="HDAC_classIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044150">
        <property type="entry name" value="HDAC_classIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF23">
        <property type="entry name" value="HISTONE DEACETYLASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="3">
        <location>
            <begin position="38"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005973-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="250" mass="28393" checksum="4FA81CE6BCA29733" modified="2013-10-16" version="1">MDHSYLLKKSYNIIFLFQWSINAAKISEIPFLIFVPNVIVQRCYLKRMRYQTGGSILASKLALKNGWGINLGGGFHHCCSSKGGGFCAYADISLIIHKLFTEEEQLKKVMIVDLDAHQGNGHERDFKGNKNVFIFDMYNAFIYPRDHPAKLAIDVAVELKPHTEDNDYLSKLMKNLEISFSKFHPDMIVYNAGTDVLKGDPLGLLDITDNGIINRDEIVFAKASQLKIPIVMLLRVVIYVPLLKLLQIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRK7</accession>
    <name>T1GRK7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02071807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRK7"/>
    <dbReference type="STRING" id="36166.T1GRK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006292-RA">
        <property type="protein sequence ID" value="MESCA006292-PA"/>
        <property type="gene ID" value="MESCA006292"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344632_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006292-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="23935" checksum="9F8DDB9BF014B000" modified="2013-10-16" version="1">MPGDEDRSDKKKSHRARHSGVSAEKKKLKKTKSVGKDETLTARQRNPKAFAINSAISAERNFRRKEDLTRRNNTSPLWTKPLMFLHQDVTREQKVFKPLKIPKTLQRSLPYKDKPKHAAVDGVKDEERIAVILSPHEQKVNKMMKMIKTNFEDKKNKEKMEMNERARKFKAEQEQEKVAYHKRQQNLKRKMCRAISKVKGKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS66</accession>
    <name>T1GS66_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02107256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS66"/>
    <dbReference type="STRING" id="36166.T1GS66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006514-RA">
        <property type="protein sequence ID" value="MESCA006514-PA"/>
        <property type="gene ID" value="MESCA006514"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_2_1_1"/>
    <dbReference type="OMA" id="CRGGYSC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006514-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11573" checksum="DCF86433D0AD3BBA" modified="2013-10-16" version="1">MSLATTRPLVSVYTEKNEATKGNTIVLPAVFKAPIRPDVVNEIHQLMRRNNRQPYAVSEEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGYSCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT39</accession>
    <name>T1GT39_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. Directly interacts with POLR2A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA006859"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005666">
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    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000073">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006859-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="17186" checksum="15EEA7048AA42111" modified="2013-10-16" version="1">MAGVLFEDIFNTKDIDPDGKKFDRVKRLHCESESFKMDLILDINTWLYPMELGDKFRLVLATTLREDGCPDSGEFNPLEEASRADSFEYVMYGKIYRIEGDDIHSEASSSRLSAYVSFGGLLMRLQGDANNLHGFEVDQHIYLLMKKLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT56</accession>
    <name>T1GT56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006877-RA">
        <property type="protein sequence ID" value="MESCA006877-PA"/>
        <property type="gene ID" value="MESCA006877"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126756_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588517" description="Farnesoic acid O-methyl transferase domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006877-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="20225" checksum="051DEEF7D0CC1889" modified="2013-10-16" version="1">MFSIIVLIVFLNLASSSHLDDCQVGLSFRNESMNKFKTFEFKYNEPKETEVLRTKVYFKYPKPVFSVYNSPHQIVFGRYNHSTNFAIFKSHDDFLKVRNPCVFKEHIHNFSGTKYVEAIFILEKKGRFTVIVDDDMVLMCETGYIFDFANITSIYISYSGSTPNVFFYDCPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT75</accession>
    <name>T1GT75_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180371">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180373">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006898-RA">
        <property type="protein sequence ID" value="MESCA006898-PA"/>
        <property type="gene ID" value="MESCA006898"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657322_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006898-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8580" checksum="19AE4E30A1DC5D7A" modified="2013-10-16" version="1">MLKTVMTDEKIIQKNKKGSKINYNQSLSTIKKDKNKLTRIDRIYEKKILGGDTLTTTTTTTSEAAALDGNDDMNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT82</accession>
    <name>T1GT82_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200218">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA006905"/>
    </dbReference>
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    <dbReference type="OMA" id="AWKLNVE"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037361">
        <property type="entry name" value="COMMD10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12333">
        <property type="entry name" value="COMM DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12333:SF0">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="174"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006905-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="238" mass="27821" checksum="2EA40F4195E97988" modified="2013-10-16" version="1">MIDSKEIQELFQSTLLWFKRKINVNEVINTNHKTVVSTHELKDVIINWIKITDRAREGIEIINKLNNETFATVLEYVHKNMNPSEDEEEQENALEDLEKLVAIPRPEFLLLIKTLSYILKRTSTFLLKPTKLQQELREKLKLDDDKIDGIIRLWIKNTKPILTNLHCGSMESKEVTDVSWMLNLQISSNCQQKERTALACLQLKTGDGNDIDLEMNHSELLNLFNQFENIQNELDNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTD6</accession>
    <name>T1GTD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTD6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006966-RA">
        <property type="protein sequence ID" value="MESCA006966-PA"/>
        <property type="gene ID" value="MESCA006966"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1126912_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006966-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="28213" checksum="765E7D303748CCF0" modified="2013-10-16" version="1">PLARKRQNFICLDCIIVVNSSEEIYRQSTLKNERFLKGGLVQQKKTESKAIPTATSNRKKSPQSKKRPQKILKTDDKIVDDFVWIDRFPCPFLNCDFASDRIQVASQHCAFTHKLFPFYYCKKCNQRFICDPEAGMETLIINPTAEDEPIIEKAQELVPQAIPSISETVPESISELIPESIPELITESVPELIPAEPQLKNIPFKPCEIKLARCCVCETVNNLRPLKPTIIPVLKRQKKERLFGYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTK4</accession>
    <name>T1GTK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014750">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007038-RA">
        <property type="protein sequence ID" value="MESCA007038-PA"/>
        <property type="gene ID" value="MESCA007038"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751806_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007038-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17332" checksum="CBBD40609E0EA7FE" modified="2013-10-16" version="1">MQPHSVNDVMSIHYDYEDKIASNNNSNSHKEENVIVLNSTETPKIQQENFAEVHISAKNLVRNCSETLCKLNNRDTISEDNSKSKISSHIIPQHDHRKIQSDTKDIEDDTRYYFVPKRQNNLQSETFPKKIIKKRNINTKLKLHNGFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTR4</accession>
    <name>T1GTR4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02074023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTR4"/>
    <dbReference type="STRING" id="36166.T1GTR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007105-RA">
        <property type="protein sequence ID" value="MESCA007105-PA"/>
        <property type="gene ID" value="MESCA007105"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007082_0_1_1"/>
    <dbReference type="OMA" id="NYCVEPP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF26">
        <property type="entry name" value="BETA-N-ACETYLHEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577127" description="Beta-hexosaminidase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="11">
        <location>
            <begin position="82"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="10">
        <location>
            <begin position="219"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007105-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="613" mass="70803" checksum="2483692395DFBCD9" modified="2013-10-16" version="1">MNIKTILWTLLILGVIGGAIAAAVVLTRSSEKETFSSDIIYGYECKSNKCVKVLIDEKNYKTAVSQPVCNIFCEENNEIGTLWPKPTGKTSVGNTMVHIDPSKIQIHGYRHEYYNVAKERFLRQITARYSDDADVINKDGKPFTVNIVTDEGDDVGKLVLETDESYKLRINENEAYITAKTFYGARHALETLAQLIVYDDIRRELQVVGNAEVNDAPVYKHRGLMLDTSRNYYSVKAIKKIIDTMGMAKLNTFHWHITDTHSFPFEMKSMPDLYKKGAYTAKKIYTHKDIAELVKYGRACGVRIIPEFDQPAHIGEGWNESLLTCFKYQPWSTYCVEPPCGQVDPSQDEVYDVLEKIYSELYDAFDAPDWFHIGGDEVSTTCWNTSTTLQNYMKSKGWQLNEEDFMKLWGEFQVKALERFDKITKTPEKVPVIFWTSTLTDVPYVDKFLDPNRYTIQIWTTGKDKKIDDLLERGYKLIMSNYDALYLDCGFGGWVTDGVNWCAPYIGWHKVYENDPKKIAGKYHAQVIGSEAAVWSEQIDDFTIDSRFWPRASALGERLWSDPKESWKMAEHRMLVIEKDLWRMELLPNPFNLNGVFKTKANVQFKRSLWYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU25</accession>
    <name>T1GU25_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007225-RA">
        <property type="protein sequence ID" value="MESCA007225-PA"/>
        <property type="gene ID" value="MESCA007225"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852244_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007225-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7337" checksum="11342F3F68A3AE6A" modified="2013-10-16" version="1">MGQEKKPEVGSMNYDENLKDWTSINNKAKRRILFSLDTKALLRVGGFETARDVWTKLSATSSGDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUN5</accession>
    <name>T1GUN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152510">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GUN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007452-RA">
        <property type="protein sequence ID" value="MESCA007452-PA"/>
        <property type="gene ID" value="MESCA007452"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_336257_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18507">
        <property type="entry name" value="BACK_GPRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF46">
        <property type="entry name" value="SERINE-ENRICHED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="13"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="632"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="690"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="827"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="848" mass="95419" checksum="90146BF3ACB04829" modified="2013-10-16" version="1">MHANLPLFNNNKTNFNLQPSQHKTLIIEEFEPDVFRQLIEYIHTGAVTLQPRTLLGVMNAADYYGLDELRRACSGFVQTCINVDTVCALLASAERYIQYKCTKSLVQKVLEFVDEHGNDVLNLGSFTLLPQHVVRLILAREEIVIPPITVSNPTETYAIVERMNAAKTTPKTPEKEATPEKKSMMRELFSFVKKPKSMGSSSSSTTTSRNRFASAFSRATISSSGSVSSSGGSPSLVRQCTYSSGTGAIPKQMSESSSGFEPRLSFKFAKMSLRLRNRFSSERKKTTTITVQQAQQQQQQNSQQVCSSYKQSDRSNNTSRGDNNNVDNTNTDAVPFELANVHFEKVGESYIKHDKSKRYNNDNHNNNNTNTNNHTNNNNAILGASTSTGSLVKRCESREPVEPRISEECEEAVAEAEKQSNEKFIPDSLRDEIERLIKPIKLEPVYVNIVELRNEINNNKKEDEDEIFHKPLNCPTIEFEPPSRRSSFDPPRSPYLENLRSSCGSTGASTDTDLLIMSRLNSGEDSFEMMESERRRESSFEGPVSTYTSYDMSRYQSTSCEDPGSSFEIVDMDQTHIEMRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRTDSKRKMSLETHFDYNPSLSPNASQATSAYVSDTHSCTTPRQRSPAFPIGRKHRNSTLGLIGAPRSPLSAQTSSNYSSRDSYDSSSSHVPLLEPKDCHLEFKPIETLKKHFPFTIKSKGEEIAKSFVCTDKRCENIFEPRPQTKTVNSGIDSGMSVLSSSGEFDPPSPRRCSSVSPKHMFMFKIVMKKVDSMPHEAAGSRKNSENLGVSNERRRSRTLEKLRRRDSRKKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV15</accession>
    <name>T1GV15_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV15"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007593-RA">
        <property type="protein sequence ID" value="MESCA007593-PA"/>
        <property type="gene ID" value="MESCA007593"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1262835_0_0_1"/>
    <dbReference type="OMA" id="NSIHWSS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037496">
        <property type="entry name" value="BEND6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BEN" evidence="7">
        <location>
            <begin position="114"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007593-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="24455" checksum="CDB753A5958256DD" modified="2013-10-16" version="1">MPDIYRQSILENSDSMDEMQKRLKVIRASQDRCSMLFESLNGVIGELEENYRLPSDKGSSDESASDKSSNHEPPRKHKSLSPVLSKKSSDNEEASYDEQYPMNDPFDANLVIIGPNGTSIGRNFYNSIHWSSSSAATRQLLVNIFPGEVLATHTLTGKPSPAFLGRERPSKNQLDPLKTSDIVYIVTIKCGVSPREVRATITTKCADSAKKYRKKRITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV98</accession>
    <name>T1GV98_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RPN1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GV98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007688-RA">
        <property type="protein sequence ID" value="MESCA007688-PA"/>
        <property type="gene ID" value="MESCA007688"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_732771_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007688-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="378" mass="43339" checksum="A8043CE0AA436874" modified="2013-10-16" version="1">MQSCFPYVPEPENVNILKVALALSRKFKQPTQSMRLALMLNDHALIEEIFTQTDTDSATKKQLAFMLARQQICLELKESTPDYDDLMEIMSNTHLNNYFLTLARELDIMEAKTPEDVYKSHLDNSRTSFGSTQVDSARQNLAASFVNGFVNAGFGVDKLLTEDGNKWIFKNKEHGMLSATASLGLILLWDVDXFVKFFNDFLQNIFLPKLHTFAIQLPLTDLYCGLKKFLKNHTNILVCTVNGKLPTLERFSLEKLLRLMDEPFDDLAIISFIAETLPKLKVLNLENLYENIEENKPNMVINVSSFKNLEKLELDKILLIDKFLRSIRAPKLKDLVIKYSYLKFEGIKGIVENTAVLRVLTFVDCIQLEKSVVTYLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVE7</accession>
    <name>T1GVE7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007746-RA">
        <property type="protein sequence ID" value="MESCA007746-PA"/>
        <property type="gene ID" value="MESCA007746"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913034_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007746-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="305" mass="36494" checksum="15897BB7A8648B8B" modified="2013-10-16" version="1">MREYNEQMEFLRDQGSLFGFDFQKIESLVEKPPEPGKENPPRNNEENIIDKDYFETFPNEALEHLYRILPKSAKLNFKPTLDGILKNRFTPEEIELRSNKRETKIREFREQMKILRDQTSHTVFDLEKIESFLNNYTGVIEDRDLDVDYFQTLDKQTLQNIYEMVPKVSRQIFLNAIKKNILTPVQRQLKRMNDVERKRRQRAAMKMQEFKNQLIAFVGQSSQFEQDFMKIELFLGNSSENNEDRDHILNYFQRFDDLALQQLFKILPTNHKEVIKPIFDIIQFKRYTPDNTGLQEVINIIPGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVK7</accession>
    <name>T1GVK7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131281">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVK7"/>
    <dbReference type="STRING" id="36166.T1GVK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007816-RA">
        <property type="protein sequence ID" value="MESCA007816-PA"/>
        <property type="gene ID" value="MESCA007816"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1116825_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47412">
        <property type="entry name" value="FI01434P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47412:SF1">
        <property type="entry name" value="FI01434P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007816-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="249" mass="27917" checksum="A31D7BE2D7D5811C" modified="2013-10-16" version="1">MIGTGNNFSPSGTYIIQQQETNPARDFVPGKMRELAQAMVSGLSGTNEAAGPGPGVPVPKTDETIQQDPVITQDNNNNVNNVPQPDMTMTNNELGEAPVSFVNNTDPEGYVNNGDDRDYDDRALMKCNDKEYVPETLQRGDFWVLKNYVRADHGELKCHESITYTTHADFTFLDNLIPLLERFQGYALCVLDYEFHILDNAFLVHKPGIKVLKKDNRRAMLTGKTNQLIKKIIFPELKIMYGTRKGCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVV9</accession>
    <name>T1GVV9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVV9"/>
    <dbReference type="STRING" id="36166.T1GVV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007927-RA">
        <property type="protein sequence ID" value="MESCA007927-PA"/>
        <property type="gene ID" value="MESCA007927"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165155_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007927-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10503" checksum="7623FF997308148B" modified="2013-10-16" version="1">MTRYSKEPDNPTKSCKSRGPHLRVHFKNNHKTALQRWSNTDFKDLDVDRLVVDHIQVKRAQCLRRRTHRAHSRIYPYMSSPYHVELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX14</accession>
    <name>T1GX14_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02119524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02119525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX14"/>
    <dbReference type="STRING" id="36166.T1GX14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008352-RA">
        <property type="protein sequence ID" value="MESCA008352-PA"/>
        <property type="gene ID" value="MESCA008352"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_7_1"/>
    <dbReference type="OMA" id="DIAGTHV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF33">
        <property type="entry name" value="AT22075P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588573" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="21750" checksum="CF39B7ABA7910510" modified="2013-10-16" version="1">MLIGLVLMALNQFCGCFAMINYTGTIFEQAGSSLSPNMSAIIVAFIQLVGSYASTALVEKAGRKILYLTSCIGTGVGLSILGLYINLSSSGYDCSSFKWVPIAAFSVTIFFSTLALLTLCFLVIAEILPPKIRALGSNICMSVLWVFCFLILKFLPFLADVLGLHGLMLLFASVCFLGAAFIVIYLPETKGRSIDEILALLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX46</accession>
    <name>T1GX46_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067209">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02067210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02067211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX46"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008386-RA">
        <property type="protein sequence ID" value="MESCA008386-PA"/>
        <property type="gene ID" value="MESCA008386"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690644_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008386-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8415" checksum="6A913DFF059B6808" modified="2013-10-16" version="1">MSVWTPPKIKKKFEMPEELLKAAYINFIDAFIDASQNSKTMQNALFALTTKKETNMTPRAVSKLLLDSPILKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXG1</accession>
    <name>T1GXG1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXG1"/>
    <dbReference type="STRING" id="36166.T1GXG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008511-RA">
        <property type="protein sequence ID" value="MESCA008511-PA"/>
        <property type="gene ID" value="MESCA008511"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521545_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008511-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="20404" checksum="9C8736464068335A" modified="2013-10-16" version="1">LTGTAIILFLSITLSFPKLLPSIRHQFDYLTLAVFVYQFLIGYLLLKGIHRENLKYIKAWIILQIFHILLVFSVFLFKCFNFEYDILDNILLVRSCDNNNVNSESIKTARIRNRLRTRTVCSELVSSAINNTVNRGTPQGGVISLLLWIVMINMILKPLEEDGVKVVDYAVCKMFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXK0</accession>
    <name>T1GXK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXK0"/>
    <dbReference type="STRING" id="36166.T1GXK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008552-RA">
        <property type="protein sequence ID" value="MESCA008552-PA"/>
        <property type="gene ID" value="MESCA008552"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2678091_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008552-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8643" checksum="7AFB01036A90F0B2" modified="2013-10-16" version="1">MVKIKFYVINIVAFGQKAKPTSNQSQREIPPKKPEGDTESIREKVLLSKGEKWRILKNISIISFAFMIQFTAFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXP1</accession>
    <name>T1GXP1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXP1"/>
    <dbReference type="STRING" id="36166.T1GXP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008594-RA">
        <property type="protein sequence ID" value="MESCA008594-PA"/>
        <property type="gene ID" value="MESCA008594"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052288_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008594-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13730" checksum="22BF1F8513227074" modified="2013-10-16" version="1">MATVCNLKLSKQLARLRTITLFRQGDALFCSFFNILLEWIMVSANVNTRNILLTKSNQVLAYALIKTLSSVNVVESFIRLERAAELVGLQVNRDKTKYMLNSRRTPQLKQSSPWIPITSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXR0</accession>
    <name>T1GXR0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXR0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008614-RA">
        <property type="protein sequence ID" value="MESCA008614-PA"/>
        <property type="gene ID" value="MESCA008614"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1449279_0_0_1"/>
    <dbReference type="OMA" id="WEMGEIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007931">
        <property type="entry name" value="TsetseEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05267">
        <property type="entry name" value="DUF725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein TsetseEP" evidence="2">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="20125" checksum="AA39679C96A8E4B7" modified="2013-10-16" version="1">MVCYQESSAALQEVATKSAEGNTKCNNILTNAQTQADNELEENRKTLDEESQDIQISLTSCDASTLPNAADALECYETQGQKNVNPVSSLSSKSRSYRRGYENTLNLAKTASDGCSYDVASESEQISAQIFNNLNSCITSGQWDPVDIPDFGPAEPTTKAPTTVSTVAPTTEDNSVPDPETTLEPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXV9</accession>
    <name>T1GXV9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deubiquitinating enzyme A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXV9"/>
    <dbReference type="STRING" id="36166.T1GXV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008670-RA">
        <property type="protein sequence ID" value="MESCA008670-PA"/>
        <property type="gene ID" value="MESCA008670"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822781_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.90">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008670-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="35506" checksum="6C7BF888B83D2E7B" modified="2013-10-16" version="1">MQNGYPPLRLSYQRGTHYNAILDPYNATVGVGLGLAGYKPEMQTQKALQLSEDLELEQTMLEDKIKTTDWEATNEAIEEQIARESYIQWCRDNLKSCTSNNSSSVGGSGSGMGGANVASGSSSASSSCATVTSAEINKGNKSCTVTATSSSTAATSSAFSAATASGSSSSSAGCSDFVSSGVGPGSNMHLNLLTSKGCNSPRDCDSDDTDMSSTSSIDHQMGEKRNSGSKKGKSRKFGKRRKQFDMKQSATDKEPQIEIAEGEIGPPSAKSPKCDATPTLESTELGKVENNSGSPSKESMNTAARLNAPCTSKQAESLNDLPISTFYQSLLESSYADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY55</accession>
    <name>T1GY55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SMB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protease inhibitor I19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02184181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY55"/>
    <dbReference type="STRING" id="36166.T1GY55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008776-RA">
        <property type="protein sequence ID" value="MESCA008776-PA"/>
        <property type="gene ID" value="MESCA008776"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012184_3_2_1"/>
    <dbReference type="OMA" id="AEIYHSG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02620">
        <property type="entry name" value="Peptidase_C1A_CathepsinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008037">
        <property type="entry name" value="Pacifastin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036201">
        <property type="entry name" value="Pacifastin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05375">
        <property type="entry name" value="Pacifastin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57283">
        <property type="entry name" value="PMP inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="SMB" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008776-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="395" mass="45242" checksum="76564A4B366AA71E" modified="2013-10-16" version="1">CCWFIFSDTICYCDDFCDRGISEDCCPDYHNFCKNIPDPITTCLHNKTLQTYQPKDTFKDGCNDCTCMNNGEIRCTQEICLTDNILVKNVNMNPRIGWTATLYPEFIDRKWSEGQLRCGPLKPSFDRVTKITHNKENLPREFKADEQWPGLISGIQDQGWCASSWIVSATSMSSDRFSIQSNGKEAVKLAPQQILACTKRQQGCDGGYLDAAWSFLNKFGVVDESCYPYEAKRGSCRIHPQKNEKTDLRAAGCSPVLEPRKRFYTMGPAYPLAAQEKDIMAEIIKSGPVQATFRVYRDFFSYKGGIYRRSAADRNINDFKYHAVKIIGWGEEYKGDNTEKYWIVANSWGSWWGENGYFKIVRGTDECEIESHVIAAWPNVLYEQIDNNIIDVHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYF3</accession>
    <name>T1GYF3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein wntless</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">A segment polarity gene required for wingless (wg)-dependent patterning processes, acting in both wg-sending cells and wg-target cells. In non-neuronal cells wls directs wg secretion. The wls traffic loop encompasses the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi, and involves clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps35 and Vps26). In neuronal cells (the larval motorneuron NMJ), the wg signal moves across the synapse via the release of wls-containing exosome-like vesicles. Postsynaptic wls is required for the trafficking of fz2 through the fz2-interacting protein Grip.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with wg; in the Golgi. Interacts with Vps35, a component of the retromer complex; wls stability is regulated by Vps35.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Presynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYF3"/>
    <dbReference type="STRING" id="36166.T1GYF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008878-RA">
        <property type="protein sequence ID" value="MESCA008878-PA"/>
        <property type="gene ID" value="MESCA008878"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022911_0_0_1"/>
    <dbReference type="OMA" id="LCFWVIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007367">
        <property type="term" value="P:segment polarity determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061355">
        <property type="term" value="P:Wnt protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009551">
        <property type="entry name" value="Wntless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449:SF2">
        <property type="entry name" value="PROTEIN WNTLESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0709">Segmentation polarity protein</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless-like transmembrane" evidence="19">
        <location>
            <begin position="126"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF06664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008878-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="399" mass="45802" checksum="F15F358782EF8A50" modified="2013-10-16" version="1">MPLPREGRVLDFSRWQQNLIGVLQVDVAFDKSVREAVKEDIILDIRLAYRNKNDPETKWTLYAHSLEKRSLDCQLYNIQDEAVFNCEMMPLFELGALHHDFYLLNIRLPMDTDMQMNTKIGHLHDLNLTVIFQNGGFTMFLTLEFEMPYMLLLSDIRQGVFYAILLSFWLIFAGEHMLIQDSGEKSTLKTYWKHLSAVVIGCFSLLIFDLCERGVQLRNPFSSIWTSRIGTHFALAFIILGGLSAGIYFLFLCAMIWKVFGNISMKRTSFSAMSQARRLHYEGIIYRFKFLMLATLSCAAVTIAAFILGQVSEGQYKWDEHLHVEVASAFLTGIYGMWNIYIAALLILYAPSHKQWPVEHSASNENIISEEIEFSNLPSDNNPSEISSLTSFSRKTAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYJ0</accession>
    <name>T1GYJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008919-RA">
        <property type="protein sequence ID" value="MESCA008919-PA"/>
        <property type="gene ID" value="MESCA008919"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2910782_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008919-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="7207" checksum="D3BAA0F176733A84" modified="2013-10-16" version="1">SERHISWTVKVYPPVSCGKDYLFLRIIENCKKRWSIDSCVVAKKVWGGIDENSQFFIVKSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ36</accession>
    <name>T1GZ36_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ36"/>
    <dbReference type="STRING" id="36166.T1GZ36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009128-RA">
        <property type="protein sequence ID" value="MESCA009128-PA"/>
        <property type="gene ID" value="MESCA009128"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929958_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009128-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14560" checksum="7AA1E1E7444C8A64" modified="2013-10-16" version="1">MMRNQARNSISGKITPIGSACKDINGNGRSFSVNCLMAISKTSKNRFHFVSQNNGEMPPPSRTKVNRAIQRLKKKKSAGSDGILPAKLLKAAGATFNDVFHIKLSSIWISEKMSEEWNVSIIYPVHKKDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ76</accession>
    <name>T1GZ76_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ76"/>
    <dbReference type="STRING" id="36166.T1GZ76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009174-RA">
        <property type="protein sequence ID" value="MESCA009174-PA"/>
        <property type="gene ID" value="MESCA009174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682312_0_0_1"/>
    <dbReference type="OMA" id="PHTCKEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17276" checksum="0C893EF88B305FA5" modified="2013-10-16" version="1">RNTNSKTTVLTNFQRGNTEKQFCDEIHLTFHERAGQGELTADQLKSTVLNPDLNDKHGFTSLHWACFYGQLSSVELLIATGRVDVNSLAPDLVTPLHLAAAGGHHEIVGLLIQNNANPNSVDIVGNTPLMYASALNHPHTCKELLKKFPDITTENEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZM0</accession>
    <name>T1GZM0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRP15-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZM0"/>
    <dbReference type="STRING" id="36166.T1GZM0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009324-RA">
        <property type="protein sequence ID" value="MESCA009324-PA"/>
        <property type="gene ID" value="MESCA009324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725067_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8056" checksum="F3F168787F454DE1" modified="2013-10-16" version="1">MDSENEAGNAGWANSIAKVLNTSKPQKRKTLVLSRAKKFTSKVTEDKPKYDFEIEGEVKEEKPEVSESTEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZP9</accession>
    <name>T1GZP9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376576">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009353-RA">
        <property type="protein sequence ID" value="MESCA009353-PA"/>
        <property type="gene ID" value="MESCA009353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834041_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7655" checksum="AB609B7559DEA110" modified="2013-10-16" version="1">MLPANFTRPIFLAMFSQCLSKNFKHLQEQTNVLRNFCKFRSGSGKGEKGGTKIMPLFKRFHAQFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1L9</accession>
    <name>T1H1L9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1L9"/>
    <dbReference type="STRING" id="36166.T1H1L9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010082-RA">
        <property type="protein sequence ID" value="MESCA010082-PA"/>
        <property type="gene ID" value="MESCA010082"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280597_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11931" checksum="76CE00EB5D6E20B6" modified="2013-10-16" version="1">MKSEVNIDFLQQSFFDFKKTTEDGMVLHILNIKNITKHLNDAGEKISESMVTKISITLPLNYSHFYCSREYNPKMERTLQNLTPRLCIEPSVDEIHRRLIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Y3</accession>
    <name>T1H1Y3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Y3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010207-RA">
        <property type="protein sequence ID" value="MESCA010207-PA"/>
        <property type="gene ID" value="MESCA010207"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2948603_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010207-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6881" checksum="F01E8AF09CC3DB01" modified="2013-10-16" version="1">MYNPFFFIFPYFSTSCNNGHSLISNQQVYAYGNLKDQCNVQKTSNINATTFINSIISLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Z6</accession>
    <name>T1H1Z6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha-macroglobulin receptor-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Z6"/>
    <dbReference type="STRING" id="36166.T1H1Z6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010221-RA">
        <property type="protein sequence ID" value="MESCA010221-PA"/>
        <property type="gene ID" value="MESCA010221"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712021_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010221-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8018" checksum="A719AC0D9CCA921C" modified="2013-10-16" version="1">MLILTVVSNFQGGAESNMAVLEVSLPISLPSGYIADKDGFANILIVERVKKVETKNEDTVIIIYFESLTEEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H265</accession>
    <name>T1H265_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H265"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010298-RA">
        <property type="protein sequence ID" value="MESCA010298-PA"/>
        <property type="gene ID" value="MESCA010298"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3351618_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010298-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="37" mass="4538" checksum="AD22A9DFC9352618" modified="2013-10-16" version="1">MFQKLIFNEEISKINEKHEKDVIDYKAKISRLIKMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2A9</accession>
    <name>T1H2A9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2A9"/>
    <dbReference type="STRING" id="36166.T1H2A9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010347-RA">
        <property type="protein sequence ID" value="MESCA010347-PA"/>
        <property type="gene ID" value="MESCA010347"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136298_0_0_1"/>
    <dbReference type="OMA" id="NKSYEAP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024857">
        <property type="entry name" value="Cappuccino"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16230:SF3">
        <property type="entry name" value="BIOGENESIS OF LYSOSOMAL ORGANELLES COMPLEX-1, SUBUNIT 4, CAPPUCCINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16230">
        <property type="entry name" value="CAPPUCCINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010347-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="13130" checksum="733D4A0AABDA4838" modified="2013-10-16" version="1">MKKILEFKSNFQNLCNRIDNLEKFVNNVNENVNKLEIAVETAEQELGVTDYSLKGLLRKPIFSKQRSSPTEEREIKSNLNENGEFVRPDIFKTEDFFVSNTTGLKIEQEEKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2P1</accession>
    <name>T1H2P1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2P1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010494-RA">
        <property type="protein sequence ID" value="MESCA010494-PA"/>
        <property type="gene ID" value="MESCA010494"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2549518_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010494-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9532" checksum="C39186644D8BB751" modified="2013-10-16" version="1">MADHVATTTAQQLHNANKSIRISPDKLNMNNNMNQNVIIRERSFIRNSNQQNTKNRRSLAIMPNNQQGNMRGGKPTMEIYRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2U6</accession>
    <name>T1H2U6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription initiation factor TFIID subunit 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2U6"/>
    <dbReference type="STRING" id="36166.T1H2U6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010551-RA">
        <property type="protein sequence ID" value="MESCA010551-PA"/>
        <property type="gene ID" value="MESCA010551"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199971_0_0_1"/>
    <dbReference type="OMA" id="GREHGAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07982">
        <property type="entry name" value="HFD_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01443">
        <property type="entry name" value="TFIID30KDSUB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010551-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11929" checksum="BA80E05D9D30A22D" modified="2013-10-16" version="1">MNPNGDNFGIHRVGALPTKAESEQTRGQVLSNFMLHLEDYQPTIPDAITSQYLNSAGFESSDPRIVRLISIAAQKFISDIANDALQHCKTRTNNSQHGREHGANKVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2V7</accession>
    <name>T1H2V7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041995">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2V7"/>
    <dbReference type="STRING" id="36166.T1H2V7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010563-RA">
        <property type="protein sequence ID" value="MESCA010563-PA"/>
        <property type="gene ID" value="MESCA010563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369287_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008112">
        <property type="entry name" value="Relaxin_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF80">
        <property type="entry name" value="FI21465P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01739">
        <property type="entry name" value="RELAXINR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23019" checksum="2ED717BDBB628103" modified="2013-10-16" version="1">MTPHIRKCKPTTDGVSSLEDLLTKPVLRYSAWMMAFLTMLGNILVLWGRFIYRDENRTVTIVIRNLAMSDILMGFYLIFIGIQDYRFRSRYHEFAMEWITSWHCAAIDFMCWAPIIGAKIYALFSFNISDDTYAWLVVFILPLNSAINPLLYTFTTPKYRNQILGRNWKKFTWSRKNTTSGTATATATGTATASNPSHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Y0</accession>
    <name>T1H2Y0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Y0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010586-RA">
        <property type="protein sequence ID" value="MESCA010586-PA"/>
        <property type="gene ID" value="MESCA010586"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349048_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010586-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="10936" checksum="76CC590448C8FACE" modified="2013-10-16" version="1">MSAFAVFDALQKLNLTSDSFFVHETSRSSSSVFYIYHTTSLVSSGGIWDLIKDEILVLASKILYAVNIWKRYQSGQKLDSDCEQDVSPDVCKTLFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H371</accession>
    <name>T1H371_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387248">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H371"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010690-RA">
        <property type="protein sequence ID" value="MESCA010690-PA"/>
        <property type="gene ID" value="MESCA010690"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963405_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010690-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6946" checksum="229E314DE0200F43" modified="2013-10-16" version="1">MITNKSTQKLGYANDIDVVGRTRDWKKKLESNDFENNKNHSINSYDVFVIRWRIPNTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3M1</accession>
    <name>T1H3M1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02091716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3M1"/>
    <dbReference type="STRING" id="36166.T1H3M1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010850-RA">
        <property type="protein sequence ID" value="MESCA010850-PA"/>
        <property type="gene ID" value="MESCA010850"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137712_0_0_1"/>
    <dbReference type="OMA" id="MEYLHVV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:006066">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010850-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="18202" checksum="8B970571755D6221" modified="2013-10-16" version="1">MCEEIEGDVLLFLTGQEEIEEACKRIKREIDNLGPEIGELKCIPLYSTLPPNLQQRIFEAAPANKANGAIGRKVVVSTNIAETSLTIDGVVFVIDTGFAKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTKPGKCFRLYTESAFKTEMQENTYPEILRSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3V5</accession>
    <name>T1H3V5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aromatic-L-amino-acid decarboxylase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02165224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3V5"/>
    <dbReference type="STRING" id="36166.T1H3V5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010949-RA">
        <property type="protein sequence ID" value="MESCA010949-PA"/>
        <property type="gene ID" value="MESCA010949"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011856_3_1_1"/>
    <dbReference type="OMA" id="ATENCCK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF76">
        <property type="entry name" value="FI02861P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010949-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="45615" checksum="5A891E3A96B3DAB6" modified="2013-10-16" version="1">MNVEEFRGHAKNIVDFIANYCKHIESKDVLPGVEPGFLPPLLQKECPEKGEPFDKIFKDFEEKVLPGMTPDSHPNYFAYFSCGLSFPGILGEMLAHGFSSRIFTWINSPSGTELENIMMNWYGKAIVFQTRCGVLQSSSSECILTCLLVARSHKVDLLRGNSNIHESEFLPKLVAYSSQDAHYSIEKAAKISFTKINLISCDDKGRMRSDLLEKSIKEDINSGLIPCFVMATLGTTNCAAFDDAEAIGKICREYKIWFHIDGSYGTNAFILPELRHFGKGMEYADSINANAGKMLLINHDSAAMWVKDSFILKKALSASAVYVDNHSNNNCIDYRNYGIALGRRMRAFKFWFVFRSYGLSGLREYVSRMLNMAKLFVKLVKEDDRFEVVNDVNLTLSLKRIAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Y1</accession>
    <name>T1H3Y1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02382483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Y1"/>
    <dbReference type="STRING" id="36166.T1H3Y1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010978-RA">
        <property type="protein sequence ID" value="MESCA010978-PA"/>
        <property type="gene ID" value="MESCA010978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1637348_0_0_1"/>
    <dbReference type="OMA" id="QDSLECN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18640" checksum="D361E77E71ECE5B4" modified="2013-10-16" version="1">MDKDFKTDLNNWALESIAFIALDTRLQCFDEKNKSPDADILINGMHKFFELSYELDIKPSLWRYFSTPKFNKLMETLDDVTDASIKYIDAAREKMKTQPPKAPAEMSILEKLIKIDRKTAVVMAMDMLFAGIDTGRTFRQLDKGLTMSLGHKVRDLPDELLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4N6</accession>
    <name>T1H4N6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02020686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4N6"/>
    <dbReference type="STRING" id="36166.T1H4N6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011251-RA">
        <property type="protein sequence ID" value="MESCA011251-PA"/>
        <property type="gene ID" value="MESCA011251"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_724769_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051139">
        <property type="entry name" value="Mediator_complx_sub13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041285">
        <property type="entry name" value="MID_MedPIWI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18296">
        <property type="entry name" value="MID_MedPIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MID" evidence="9">
        <location>
            <begin position="145"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011251-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="382" mass="41716" checksum="4430605C32E399A1" modified="2013-10-16" version="1">MGGDLMTVPSVGSPGSAAASPHPNSQLSQPSSVPPVDQLMSPRPPPSVPTNLQQPNTPLEHLLDKNTPAPTPTDQHDNKSITASPYIHQNPSIEAPLLAHLASGLCEPQPIPALIVGHEKDWLSVAPYAIHYWDKLLLEPYSYSKDIAFMVVCPDNEDVLANSKMYFKELSTTYEMCRLGRHTPMTKWNGFLPVNSGCILPNQQRTSLDDWFDSLPDRRFGEQLKMYAHALMRTGGNTGVNIGAPTSPQSHESSMTAEIKPPISPIKQEPGTESNGSTVTNEPATSNAAATILHDPMSEDDDINPPIIVVYLVEPFTYGNDSPDVQRVACLALLRSFSEMLNALPESIRGISLTGFGTAANIEHFLKSKDDKNRLPYKMFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4S5</accession>
    <name>T1H4S5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H4S5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011293-RA">
        <property type="protein sequence ID" value="MESCA011293-PA"/>
        <property type="gene ID" value="MESCA011293"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1528920_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011293-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="20014" checksum="8546F1C4FE3878D5" modified="2013-10-16" version="1">MIIFLYRLRREQLDSVNKIVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLNSSNNNSNKKGKSSGSRLYGRLSFVDDSVSGENDSNFDLADFHIVGTCRDLEKSFLRLTKAPLPSEVRPIDVLQHSLINVKTKWKEGNGQDYYYACDQLKSIRQDLTVQGIRNEFTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5I6</accession>
    <name>T1H5I6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily B member 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Endoplasmic reticulum DNA J domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins. In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1. Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with HSPA5/BiP; interaction is direct. Interacts with ERN1/IRE1 (via the luminal region). Interacts with DERL1.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02099011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5I6"/>
    <dbReference type="STRING" id="36166.T1H5I6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011564-RA">
        <property type="protein sequence ID" value="MESCA011564-PA"/>
        <property type="gene ID" value="MESCA011564"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_18_0_1"/>
    <dbReference type="OMA" id="CHREQVP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="6"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011564-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="58" mass="6629" checksum="07F17D4B11BB5186" modified="2013-10-16" version="1">MVKETGYYDILGVSPNASSYELRKAYRKLALKYHPDKNPNESEKFKQISQAYEVSGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5L1</accession>
    <name>T1H5L1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5L1"/>
    <dbReference type="STRING" id="36166.T1H5L1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011592-RA">
        <property type="protein sequence ID" value="MESCA011592-PA"/>
        <property type="gene ID" value="MESCA011592"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1965054_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032062">
        <property type="entry name" value="DUF4803"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890:SF1">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16061">
        <property type="entry name" value="DUF4803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011592-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="15374" checksum="1E3F1B28F5BD38A6" modified="2013-10-16" version="1">MYNVCRSSTTDKRECHQELLYIRMKNSHWELGVNYDQLTRVVQGYIVNEVNINEVYTCTDDCKYHVDREVYGCYKKDSYCPQDRGTGRIIRCEFDTNKDHVTLCPSDNPARRYEYFETNRGKKHNNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5T0</accession>
    <name>T1H5T0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Cdt1 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5T0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012063-RA">
        <property type="protein sequence ID" value="MESCA012063-PA"/>
        <property type="gene ID" value="MESCA012063"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023373_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071163">
        <property type="term" value="P:DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08767">
        <property type="entry name" value="Cdt1_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08674">
        <property type="entry name" value="Cdt1_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1420">
        <property type="entry name" value="DNA replication factor Cdt1, C-terminal WH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045173">
        <property type="entry name" value="Cdt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032054">
        <property type="entry name" value="Cdt1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038090">
        <property type="entry name" value="Cdt1_C_WH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28637">
        <property type="entry name" value="DNA REPLICATION FACTOR CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28637:SF1">
        <property type="entry name" value="DNA REPLICATION FACTOR CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08839">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16679">
        <property type="entry name" value="CDT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01075">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="4">
        <location>
            <begin position="69"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012063-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="446" mass="51717" checksum="A68C2D3E744E391D" modified="2013-10-16" version="1">PVKTFKTPTKLLPPTPGKNELMSPKRTEVSKRLMFSPSKDGSPSKLITQPAYQKFLALSEKPSSSKLQLPFKYRFLHEVFRCLDQVCAMFFKRKECITFKKLKPAVQRVLRKNFHESHLAQIVAILPDAYKFSQTKMRNYGSASKADYFQLIITPXQVFKMQQLSGIDENDVLKSDENSAMSAGVLLQRLQRFENVLMQRVHREHIKFLKTLNPPIILDETKKVTRWHPEFDLENVPEIERGYISTTCEIFICQRYSFNSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPELSRHLRNVFISEKKGVLPLEIVLKKIENSFRTSLTLTQIEEHLRLLSKECPEWVSFHEIRKTMYVKIVKDYDLKKIVEKLENRQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6W5</accession>
    <name>T1H6W5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EnsemblMetazoa" id="MESCA012447-RA">
        <property type="protein sequence ID" value="MESCA012447-PA"/>
        <property type="gene ID" value="MESCA012447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392234_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="11072" checksum="F2F714C2B9DD296A" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCLDILPDNCQEVYDEEEIIHKPYPICCPLYCKLKKRMNRLKSTFIRHRLLLHLKHNLLXISNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H730</accession>
    <name>T1H730_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Protein N-terminal glutamine amidohydrolase</fullName>
            <ecNumber evidence="4 8">3.5.1.122</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein NH2-terminal glutamine deamidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N-terminal L-glutaminyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:50680"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="Rhea" id="RHEA-COMP:12777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="EC" id="3.5.1.122"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NTAQ1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.122" evidence="4 8"/>
    <dbReference type="STRING" id="36166.T1H730"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012512-RA">
        <property type="protein sequence ID" value="MESCA012512-PA"/>
        <property type="gene ID" value="MESCA012512"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1598667_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.10">
        <property type="entry name" value="Protein N-terminal glutamine amidohydrolase, alpha beta roll"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037132">
        <property type="entry name" value="N_Gln_amidohydro_ab_roll_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039733">
        <property type="entry name" value="NTAQ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023128">
        <property type="entry name" value="Prot_N_Gln_amidohydro_ab_roll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035:SF0">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09764">
        <property type="entry name" value="Nt_Gln_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein N-terminal glutamine amidohydrolase alpha beta roll" evidence="9">
        <location>
            <begin position="52"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="19202" checksum="9CCC817F8AC87B3E" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDYHVFFMHNPSPNRCLVFDLDTTLPFPTYFHKYVTETFRSDLALKAEHRVIPADTYLASFASDRRHMRRPDGSWIKPPPAYSPSNGPGEVYCLSEFVQKFYKSPNIVSQQMNQNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H748</accession>
    <name>T1H748_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H748"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012530-RA">
        <property type="protein sequence ID" value="MESCA012530-PA"/>
        <property type="gene ID" value="MESCA012530"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025895_1_1_1"/>
    <dbReference type="OMA" id="HCQINAH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012530-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="305" mass="33564" checksum="5FE5B62B7E923C7D" modified="2013-10-16" version="1">XLSVASKSGYSLYSSLDKTFHCLSDEVFIIKRLFKSSLVAIGSPKLIVCHFKRQSEICNYSYSNTILAVKLNRERLVVCLEESLYIHNIQDMKVVHNIRDTPSNPTGLCALSSSSEHCYLAYPGSVTAEVQIFDAINLHAKTMILAHDSPLAAIAFSSSGTELATASEKGTVIRVFSSLDGTKLFELRRGLKRCVSIASLSFSTCNDYLVSSSNTETVHIFKLDRSLAENETKPSNDDWMGYLSKTVSSYLPTQVANVFSQGRAFTSVALPEAGVRRICAIATIQKTLRLLVASQDGYLYVYSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7F3</accession>
    <name>T1H7F3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cysteine dioxygenase</fullName>
            <ecNumber evidence="4 12">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="12">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="4 12"/>
    <dbReference type="AlphaFoldDB" id="T1H7F3"/>
    <dbReference type="STRING" id="36166.T1H7F3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012690-RA">
        <property type="protein sequence ID" value="MESCA012690-PA"/>
        <property type="gene ID" value="MESCA012690"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079443_1_0_1"/>
    <dbReference type="OMA" id="YTENQVT"/>
    <dbReference type="UniPathway" id="UPA00012">
        <property type="reaction ID" value="UER00537"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000045">
        <property type="entry name" value="Cysteine dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0883">Thioether bond</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="10">
        <location>
            <begin position="93"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012690-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22711" checksum="69A6FECDEC9F40F0" modified="2013-10-16" version="1">KPLKYGPQIESLQELITALYKEFETDYVDIEMVNHIMLSYKSDPSEWRKFAKFDRYKYTRNLVDGGNGKFNLIILCWGEGHASSIHDHSDSHCFMKMLQGQLTETKFEATEWKERFQQSNSKACELQEIGKTTIGTNDVCYINDNIGLHQVENPSHTDTSVSLHLYCPPFNSCSVFQKNLEKMTCPVTFWSKYGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7K8</accession>
    <name>T1H7K8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L38</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7K8"/>
    <dbReference type="STRING" id="36166.T1H7K8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012745-RA">
        <property type="protein sequence ID" value="MESCA012745-PA"/>
        <property type="gene ID" value="MESCA012745"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152057_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022618">
        <property type="term" value="P:protein-RNA complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002675">
        <property type="entry name" value="Ribosomal_eL38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038464">
        <property type="entry name" value="Ribosomal_eL38_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01781">
        <property type="entry name" value="Ribosomal_L38e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012745-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="40" mass="4653" checksum="59E164B5A090EAAF" modified="2013-10-16" version="1">STKFKIRCSRFLYILKVTDKEKADKLKQSLAPGLQIKEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA04</accession>
    <name>T1GA04_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000047-RA">
        <property type="protein sequence ID" value="MESCA000047-PA"/>
        <property type="gene ID" value="MESCA000047"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103919_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576752" description="Protein TsetseEP domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000047-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18913" checksum="CF22E1ABABEC0B37" modified="2013-10-16" version="1">MNSLAIFCVLGLFGGLTLASPSSRLPNNLFYIVNLTIAENDAKIDYYSVQVNLTENIFERFTQSLLYKNQLISRTLFLDERNLTNAVFLNFTNTNCIIENDKLPELAAVQGSISGCVNNATKNGSFMFTSVKNNLKQAREENYNIRNTIDTCVTSYTSGYNLTDCVNDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA51</accession>
    <name>T1GA51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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                <person name="Hughes D."/>
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        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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                <person name="Hughes D."/>
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    <sequence length="136" mass="15662" checksum="0814A9E43E78E6E3" modified="2013-10-16" version="1">MEAYSLFQQIIRCGDLESYPLLEFLDLSHSHIQEIEDDTFGRLEMLETLFLDNNQLKSIPSSLPTSLEHLFLQSNEITEVLKTEGSLLDTSTNLPATTTTTPELSRVDQLNEQEKLHSTHIQDRQSEIRFINKNEI</sequence>
</entry>
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    <accession>T1GAN8</accession>
    <name>T1GAN8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
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    <reference evidence="2" key="2">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
            <begin position="40"/>
            <end position="87"/>
        </location>
    </feature>
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        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20718" checksum="D05B95CA14546F32" modified="2013-10-16" version="1">MLPMAYATMGLPVSEESSLTTKPKIGFSIDSIVGMNRRLVSPERRSSSPSPHIIEDDEDIPVSRKCNSRSSSVRTPSPHASCSSNVSVKSPIALSAGPVRPFPLPPKQMGFSTPSESAPANIQPPPHFLAAQFQMAAALAHGHHPPPGFPMTHHPTGFPVRDSYPLYPWLLSRHGRFFPHRFPGSKYNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAS3</accession>
    <name>T1GAS3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000333-RA">
        <property type="protein sequence ID" value="MESCA000333-PA"/>
        <property type="gene ID" value="MESCA000333"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1572428_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000333-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="18835" checksum="82C5B2348944E9EF" modified="2013-10-16" version="1">MFSSPQTQNQSSNMGITRRISSLFTNTVALLTIVNKKWGGSANVCTNISQAIESCIKFTVLNANTSKSIENFHQISYRVVLKIKKNSFTQTSPLILFLRVLCTGASSVKSKNPSILNIVLPNVYENISYLNGPLKSGSPSKIMRKIEAVWYNKRFQGVLEISAPIFAKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAT3</accession>
    <name>T1GAT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000344-RA">
        <property type="protein sequence ID" value="MESCA000344-PA"/>
        <property type="gene ID" value="MESCA000344"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266808_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000344-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11513" checksum="BEFC6341C619E366" modified="2013-10-16" version="1">MESEHHLPIHKKGDEKKIAMKESLLNVVCKILASILCEKLKPHILKIFGSYQCGFILNRSTSEIFTLRQIWKRITSSKLTDISSSSSSGNLSTHRAGAICSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAV3</accession>
    <name>T1GAV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02181610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GAV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000368-RA">
        <property type="protein sequence ID" value="MESCA000368-PA"/>
        <property type="gene ID" value="MESCA000368"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_751378_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13884">
        <property type="entry name" value="CuRO_2_tcLCC_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="30"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="230"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000368-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="42685" checksum="FC185DA5AFCACBEB" modified="2013-10-16" version="1">MYTNTQRNLNPYTYLYDVDNEILDINEWPDKNLLINGKGRNQSSKDINQDELAKFKVDEGTFYRYRLVFTGYSQCIVQVSIDNHTLIVIQSDGEDLQPIEVESILLTSGERYDFVLKANQPLGNYWLRVQGKDSDCKGLYQAALIQYKDDIPLTISPFDSYELNGVQLNSDFERNNDIDIPIYQARSLYMYPKDMENVSKTFYFHLTSKNNDSFMNNIKFKTNEMVSLLNITSQDLANFTCSREDMEKSQNCTTELCVCPQMVEFPYQKLVEIVVINDLKGTHPIHLHGYSFYVVGQGDLSSETDXQNLWVLAHNYSGGCSIIWEILHNYGVYSIKFGVANAIMGYYNYEVASKILWRPPMIWLSLGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAY6</accession>
    <name>T1GAY6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000403-RA">
        <property type="protein sequence ID" value="MESCA000403-PA"/>
        <property type="gene ID" value="MESCA000403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751810_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="16304" checksum="E7DC46179EF352D6" modified="2013-10-16" version="1">MNSNLAQLIISHVFVTPAPTNTYIPPVPQTLKSETFSTGQVQTNTFLPQVKANLSPPPTSFTSSQNSYSSQPSGQESFNLQQNSFDQQLTFNVQQPQQAFNVQQQETPLQNTFNAALKALDSPKTSSTFIEGCKVSQNSLSDFGEVTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBU3</accession>
    <name>T1GBU3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02098106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBU3"/>
    <dbReference type="STRING" id="36166.T1GBU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000732-RA">
        <property type="protein sequence ID" value="MESCA000732-PA"/>
        <property type="gene ID" value="MESCA000732"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1016669_0_0_1"/>
    <dbReference type="OMA" id="NIKLAFM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF265">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR DM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="85"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000732-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="274" mass="31034" checksum="391400B8216ADBAB" modified="2013-10-16" version="1">MERLTESAAAAAASSQSQPHFLMLEMMTSTASTNPDDSDAFLNGTLNGTFTTDIESTEPVMRYTLLQMIILSIFAVLLSILTTIGNIMVMVSFKIDKNLQTISNYFLFSLAVADFAIGIISIPLFSISTILGYWPFGAIICDSCVTRPLTYRAKRTTKRATAMIAAAWIISLILWPPWIYSWPYIEGKRTVPENECYIQFIETNQYITFGTAIAAFYFPVTVMCILYYKIWRETKKRQKDLPNLQAAGKKDSSKRSNSRFFWAQLFKTFPQAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBZ0</accession>
    <name>T1GBZ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000783-RA">
        <property type="protein sequence ID" value="MESCA000783-PA"/>
        <property type="gene ID" value="MESCA000783"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870164_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000783-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7751" checksum="1BB3FA772D7C6B0B" modified="2013-10-16" version="1">MKKYTIIFIKKYAKRKLYPHDINFKGEAHQTMPNNVTEHLDLCKTGKNQHHNYHQVKPNTWLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBZ1</accession>
    <name>T1GBZ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02014956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBZ1"/>
    <dbReference type="STRING" id="36166.T1GBZ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000784-RA">
        <property type="protein sequence ID" value="MESCA000784-PA"/>
        <property type="gene ID" value="MESCA000784"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_0_3_1"/>
    <dbReference type="OMA" id="QYDWGTK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000179">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588179" description="Lipase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000784-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="386" mass="43749" checksum="8108666F6158FDB1" modified="2013-10-16" version="1">MHCILWIILELVVEANTLKIETFYYNAPSKIKADGYPVEIHNVLTKDGYILEIHRIPFSPKNKSERKQAIFMQHGLYATGDNFILADPSQAIAYLAADAGYDVWLGNNRGTFYSKKNIYFSPSNPLFWNFTFGETSVYDVSASIDYILNITQQNKIHYLGHSLGTTSYLVLLSMRPEYNSKIKSGHLLAPISAIKNLKTPIGKVLSPIFGHPDPDRSYTGFEFLPHFLPLNIFLANFCAQPGILSICKAVISLYIGPSKKYTNDSLVPVIFATFPGGSSYNEGIHLTQMVNTKRFQMFDWEQYGSKVPPEFPIENVQAPTFFYYSSGDYLSSELDVENLLKSLNKDVVAGTWFKDDGEWSHFDYLAANDLKTILNDDILENIRKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC82</accession>
    <name>T1GC82_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000885-RA">
        <property type="protein sequence ID" value="MESCA000885-PA"/>
        <property type="gene ID" value="MESCA000885"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2099667_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000885-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13858" checksum="D3D28935C6302FD0" modified="2013-10-16" version="1">MKLHNSKSEKQQDCWFEWHPGTFQDSRHEFQQCFPPTAPPNMEPKFRSRHFPSTINKPNTRRESHKIPRDHNTKFLDILRVQSAISLYSCYSFEEEYAALGILRQFEPFTRRNLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCF9</accession>
    <name>T1GCF9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADAM10 endopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.81</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase of broad specificity.</text>
            <dbReference type="EC" id="3.4.24.81"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.81" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02071989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02071990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02071991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCF9"/>
    <dbReference type="STRING" id="36166.T1GCF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000971-RA">
        <property type="protein sequence ID" value="MESCA000971-PA"/>
        <property type="gene ID" value="MESCA000971"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_738810_0_0_1"/>
    <dbReference type="OMA" id="MYESATH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049038">
        <property type="entry name" value="ADAM10_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF2">
        <property type="entry name" value="KUZBANIAN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21299">
        <property type="entry name" value="ADAM10_Cys-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13574">
        <property type="entry name" value="Reprolysin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="7">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="6">
        <location>
            <begin position="150"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000971-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="427" mass="47499" checksum="5D180A36E6DC7BE7" modified="2013-10-16" version="1">RKLTLKFYYRDFTGGTLGLAWVASASGASGGICEKYKTYTETVAGQYQSTKRSLNTGIITFVNYNSRVPPKVSQLTLAHEIGHNFGSPHDYPLECRPGGISGNFIMFASATSGDRPNNSKFSPCSIRNISNVLDVLVGNVKRDCFKVSEGAFCGNKIVESGEECDCGFNNEECNDHCCYPRLITDYEYGMNVSAKGCARRANTQCSPSQGPCCLSDSCTFVPAIHSMKCKEETECSWSSYCNGTTPECPETKPRDDKTKCNNECFLTSTIVPQIDKRQLCQLACQDGNNTNTCRSTSEFAHLYGLPTGGISLRPGSPCDNFQGYCDVFLKCRAVDAEGPLVRLKNLLLNRETLHSVAQWIVENWYAVVLLGIGFIIFMGIFIKCCAVHTPSSNPKKRPARRISETLRRPMNTLRRMVIIYVLFWKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCG0</accession>
    <name>T1GCG0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF243 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000972-RA">
        <property type="protein sequence ID" value="MESCA000972-PA"/>
        <property type="gene ID" value="MESCA000972"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052295_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF243" evidence="1">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000972-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13520" checksum="F2B890227AAE2D6E" modified="2013-10-16" version="1">MIIQQCHVYFLFSNRKPIIPSPPQKHYKIVFIKAPQPPTTPLPSIPPPQVNEEKTIIYVLVRKLEETPDLVIPTPASTSPSKPEVYFIRYKTEKEQGGGDGQGYEKMKCARAGLRGNEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCP7</accession>
    <name>T1GCP7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02053111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001067-RA">
        <property type="protein sequence ID" value="MESCA001067-PA"/>
        <property type="gene ID" value="MESCA001067"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530036_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001067-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9541" checksum="55A4D77552AF75D2" modified="2013-10-16" version="1">MSNLNGQYLGGNNGSGIISPNNKFQGIYWNTFNGDEYSLKSYILKEIQQSDNRNFAKAYSRIFKSLFIPEIAGLANTRILHLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCT6</accession>
    <name>T1GCT6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001109-RA">
        <property type="protein sequence ID" value="MESCA001109-PA"/>
        <property type="gene ID" value="MESCA001109"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3399817_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576825" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001109-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="31" mass="3480" checksum="955E096DED9A7020" modified="2013-10-16" version="1">MKFKILLFTVFSTLSLTLGNPTSTEPPFYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDC8</accession>
    <name>T1GDC8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Laminin EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02042715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDC8"/>
    <dbReference type="STRING" id="36166.T1GDC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001312-RA">
        <property type="protein sequence ID" value="MESCA001312-PA"/>
        <property type="gene ID" value="MESCA001312"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_475378_0_0_1"/>
    <dbReference type="OMA" id="VECHACE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000074">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000065">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000101">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000130">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000138">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000145">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013015">
        <property type="entry name" value="Laminin_IV_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF375">
        <property type="entry name" value="LAMININ SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21199">
        <property type="entry name" value="LAMININ_IV_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51116">
        <property type="entry name" value="LAMININ_IVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin IV type B" evidence="14">
        <location>
            <begin position="28"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="13">
        <location>
            <begin position="235"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="13">
        <location>
            <begin position="313"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="13">
        <location>
            <begin position="422"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="13">
        <location>
            <begin position="525"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="527"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001312-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="63836" checksum="23C1EE505ED68079" modified="2013-10-16" version="1">MCDVVTGQCRCRENMSGRRCESPIQKHYIPFIPIIHEAEAPGTQCFGYMNHNCSRYEQNKDYMVIQSGSEVEFTVDDIPKTMTYDVQIKYMTQSPGQISDTYITLIRPETYEYSSESAICSPNEPQYEQRVPLNLPDNQRQVVALSDVCLEKGKTYRVRLHFERFRGPQDENSGVILVDSLNIIPKIIPENHVFPNEIFGKIYQERHCQYDSTDPTCADMNKHIALVIRNGSVSCDCDPTGSLSKQCEDYGGSCSCKPNVVGRTCNVCAVGTYGFGPDGCKACDCNSIGAKDNNCDVLTVSTWILEFPNCMICNCNGHTNNCDALTGECLNCEHSTTGFNCDSCLEGFYGDPSWASDIGCRPCRCPDTVASNHSHAVDCQLDRRNNNMQCICKEGYAGTKCEECADNYFGSPEIPGGSCELCDCNDNINPRDYGNCDAKTGTCLKCLYDTTGEHCEFCKDGFYGNALEKDCRPCECELLGTNSTIKYCDRFTGHCPCLPNVIGMTCDSCIENHWKIASGEGCEHCDCDPTGSLREQCNPYDGQCECRTGFGGRQCNQCQRDFWGDPNVECHACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEE5</accession>
    <name>T1GEE5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEE5"/>
    <dbReference type="STRING" id="36166.T1GEE5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001707-RA">
        <property type="protein sequence ID" value="MESCA001707-PA"/>
        <property type="gene ID" value="MESCA001707"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1706287_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="2">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001707-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17305" checksum="A2CE11D47CE6C5B4" modified="2013-10-16" version="1">MKISKTHPQVDGQKYTGQGRSKKVARIEAAATALRSFIQFKDGAILQPLKPTLNLDFTSDEHLENEVARGVVAVEGQKKVPDKGPVMLLYELFNDVHFDCSTIDGAQNNCRFKMTVQVNNCKFEGTGVGKMKYLKYLETLENDYPESHNHGWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GET2</accession>
    <name>T1GET2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 10"/>
    <dbReference type="EMBL" id="CAQQ02070568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GET2"/>
    <dbReference type="STRING" id="36166.T1GET2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001853-RA">
        <property type="protein sequence ID" value="MESCA001853-PA"/>
        <property type="gene ID" value="MESCA001853"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_814538_0_0_1"/>
    <dbReference type="OMA" id="MYLANPD"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001853-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="341" mass="38899" checksum="28EC5E04ACCD76EA" modified="2013-10-16" version="1">MIEGTPGKPYGGLLAHFNLVEANMKYRREEVSALLKPHEKVMSITNFPRLGCPLFTSPEYLPTPENTLSAARSLYFPDEGIYPGHPRFKTLTRNIRMRRGEKVQIKLKVFKDKNTILPVEGAPENEPDVVHLDAMGFGMGCCCLQLTFQACNIEEARTLYDQLTPLCPIMLALTAASPVYRGYLTESDCRWNVISASVDCRTDEERGLKPLKENKFRISKSRYDSIDSYLSKDGEKYNDVPLIYDEAVYNRLREGGIDHLLAQHIAHLFIRDTVSLFSEKVCQNDKEDTDHFENIQSTNWQTMRFKPPPPNSSIGWRVEFRPCEAQITDFENAAIVCFVVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF84</accession>
    <name>T1GF84_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002016-RA">
        <property type="protein sequence ID" value="MESCA002016-PA"/>
        <property type="gene ID" value="MESCA002016"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576585_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002016-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9028" checksum="A176A51B286DC990" modified="2013-10-16" version="1">MRWRGSGKQIYRPESADIYFSGRSTDSIDAVSVFEIIFKVRSSTGTPSTTIYDISDCVGCFTITASSMKNIQEKGRMFAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG26</accession>
    <name>T1GG26_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172673">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002331-RA">
        <property type="protein sequence ID" value="MESCA002331-PA"/>
        <property type="gene ID" value="MESCA002331"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796896_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002331-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7783" checksum="3DD9AEE843B0BD1B" modified="2013-10-16" version="1">MKTYHHQSAGFVGIPAELLKAASKNFIDGFHQLLGPFLEYTKINTIHKRKKPFPFPQPTPLGNPFYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG51</accession>
    <name>T1GG51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001188">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG51"/>
    <dbReference type="STRING" id="36166.T1GG51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002360-RA">
        <property type="protein sequence ID" value="MESCA002360-PA"/>
        <property type="gene ID" value="MESCA002360"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624819_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071333">
        <property type="term" value="P:cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046327">
        <property type="term" value="P:glycerol biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019543">
        <property type="term" value="P:propionate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal" evidence="1">
        <location>
            <begin position="28"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002360-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8778" checksum="C4503A1366317CAA" modified="2013-10-16" version="1">MEKRGQVPSIFHVNRFRKSSQGKFISIALKTKEKTIPAPNYCVKGTLGNRISPADFVQAAAERFPGCMRGRTMYDVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGK7</accession>
    <name>T1GGK7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGK7"/>
    <dbReference type="STRING" id="36166.T1GGK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002529-RA">
        <property type="protein sequence ID" value="MESCA002529-PA"/>
        <property type="gene ID" value="MESCA002529"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055524_12_0_1"/>
    <dbReference type="OMA" id="CCEENIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF478">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002529-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17801" checksum="DC0E324C0D793B56" modified="2013-10-16" version="1">MLFMSEFLVGAVAFAFRNALTRTLSNEMRFAIENHYNASDRGSLASPSVASIWDNIQTNFECCGVTSYEDWYNIKAWSELWFDKGCVHSFQNWIVKHLHVVGTIGLVIASIQLIGLLCSMLLFCSVKRRSKRSPESYKSYSPSSADLHSKNVSSWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGR7</accession>
    <name>T1GGR7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGR7"/>
    <dbReference type="STRING" id="36166.T1GGR7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002595-RA">
        <property type="protein sequence ID" value="MESCA002595-PA"/>
        <property type="gene ID" value="MESCA002595"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471649_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002595-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10302" checksum="AEA877B094D3129C" modified="2013-10-16" version="1">MVKKRYSAITVEHSLYKWVKVFIIPKQQAETVAQMLVDNWISRFSVTIELHSDQGRNLESNVFLKMCQILNISKTRTTRIYSAIIVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI10</accession>
    <name>T1GI10_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02121550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI10"/>
    <dbReference type="STRING" id="36166.T1GI10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003077-RA">
        <property type="protein sequence ID" value="MESCA003077-PA"/>
        <property type="gene ID" value="MESCA003077"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002753_3_4_1"/>
    <dbReference type="OMA" id="HTIHTHQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF471">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588306" description="G-protein coupled receptors family 2 profile 2 domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003077-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14069" checksum="904C6C8E4B526E2D" modified="2013-10-16" version="1">MLLSVLIALGLLRGMQTNSNTIHQNIVLCVFFAELLFFIGIQSRRNLLENEFPCKLIAICLHYAWLAAFAWTTVDCIHLYRMLTEMRDINHGPMGFYFSMGYGAPAIVVGLSVGVRAHEYGNSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI22</accession>
    <name>T1GI22_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003089-RA">
        <property type="protein sequence ID" value="MESCA003089-PA"/>
        <property type="gene ID" value="MESCA003089"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052283_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003089-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="14223" checksum="2C6032CFE6FFC371" modified="2013-10-16" version="1">MYICISYLLYEHLLPFLFKTCIILLVFHALKKSATEYVFTASSKKDCIPVEHTYFLKNPFSEYVFATYSKLIFAAWEMLPKLRTIQLRCEFTIRKSNFTPVQNYVTPKLRQISFRCGELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI35</accession>
    <name>T1GI35_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein disulfide-isomerase A6 homolog</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02394560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI35"/>
    <dbReference type="STRING" id="36166.T1GI35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003102-RA">
        <property type="protein sequence ID" value="MESCA003102-PA"/>
        <property type="gene ID" value="MESCA003102"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030311_0_0_1"/>
    <dbReference type="OMA" id="QCAAAHP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03001">
        <property type="entry name" value="PDI_a_P5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000032">
        <property type="entry name" value="Protein disulfide-isomerase A6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000050">
        <property type="entry name" value="protein disulfide-isomerase A6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588323" description="Protein disulfide-isomerase A6 homolog" evidence="13">
        <location>
            <begin position="18"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="10"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="126"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003102-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="358" mass="39858" checksum="8D197D571A6AE74A" modified="2013-10-16" version="1">MKQYFLIFAVLFGLSSGMYTSRDDVIELTPSNFDKLVTKSNEVWVVEFYAPWCGHCQQLVKEYNKLATALKGIVKVGAVNADEHKSLGGQFSVRGFPTIKIFGADKNSPIDFNGQRTASAMADSALAEAKKIVNKRLGGGSGSSSGGSNSKDDVIELTDDNFDKLVLNSDEPWLVEFFAPWCGHCKNLAPQWAKAASELKGKIKMGALDATVHQSKAAEYGIRGYPTIKFFPAGKKSKSDVQEYDGGRQASDIVSWGLDKYEDIVEAPELYEVTDEKTFNKACDGVSICVVSVLPHILDCDANCRNKYLDTLKEMGEKYKKKQWGWIWSEAGFKLTLKKLWKWVDLVIQQWQLLASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI49</accession>
    <name>T1GI49_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02025831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI49"/>
    <dbReference type="STRING" id="36166.T1GI49"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003116-RA">
        <property type="protein sequence ID" value="MESCA003116-PA"/>
        <property type="gene ID" value="MESCA003116"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_8_1"/>
    <dbReference type="OMA" id="NACADEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF103">
        <property type="entry name" value="CYTOCHROME P450 6BS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003116-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24578" checksum="AA4D609ADFF67E3D" modified="2013-10-16" version="1">MLLCNRTVMFYFLVVKLLIVLTAFAVYKVRKTFLYWNDRGVNYEEPVFPFGNMKGLGKSGHFSEYNVKLYEKFKTKGRYFGMFIFLNKTLVLTDLDLIKTIFIKDFQIFNERGIYHNKEVDPISEHLLTIDGKSWKVMRSKLSPTFTSAKMKFMFPTVQKVGEEFVEVLKAVVGNGNEFEIKELLARFTTDVIGTCAFGIECNSLKDPNAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIK5</accession>
    <name>T1GIK5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02065463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003285-RA">
        <property type="protein sequence ID" value="MESCA003285-PA"/>
        <property type="gene ID" value="MESCA003285"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052278_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13703" checksum="6F978A8337C5DEC7" modified="2013-10-16" version="1">MDPVDVSALISEFRTQMDIIRKASAVVDNIIAKFESINFVPFHPPTTVPPVKCKNTEILTPEMSKKLCIPPVYFEDVRLRPKYNVLYTYTFRKIVYVYNTFRNLSDGDLTPTVLEGTGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ34</accession>
    <name>T1GJ34_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminopeptidase N</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ34"/>
    <dbReference type="STRING" id="36166.T1GJ34"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003475-RA">
        <property type="protein sequence ID" value="MESCA003475-PA"/>
        <property type="gene ID" value="MESCA003475"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782110_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577410" description="Aminopeptidase N" evidence="1">
        <location>
            <begin position="18"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003475-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16667" checksum="CAE275E71560F000" modified="2013-10-16" version="1">MKLCITLLALCLGVALGAPPTIISKTLDTIHLQKAEQPNFRLNTDFYPISYDITLKPYLRSEDGDKQFTFDGESSVIFSTKKEGLRSVTLHVRNLDNLKVDLKETSTGEIIWIDHIGEVDKVTDKWTLTIETPFKKDVEYTLHFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJQ7</accession>
    <name>T1GJQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GJQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003707-RA">
        <property type="protein sequence ID" value="MESCA003707-PA"/>
        <property type="gene ID" value="MESCA003707"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003707-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27122" checksum="990C94819848C007" modified="2013-10-16" version="1">MSFCIFEYVYFARSDSLFEGQMVYSVRLQCGRQLARESPVSADIVSSVPESGTAAAHGYARESGLHFAEVLCKNRYVGRTFIQPSTRLRQLGVAKKFGALSENVAGKRLVLIDDSIVRGNTIGPIIKLLRDAGATEVHIRIASPPLLYPCYMGINIPTREELIGNRMNPEQLAKHVVWHTXXXXXXXXXXXXXXXXXDSLAYLSVEGLESAVKLNSQTKGNREQSGYCTACLTGEYPGGVPDDLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJZ2</accession>
    <name>T1GJZ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003802-RA">
        <property type="protein sequence ID" value="MESCA003802-PA"/>
        <property type="gene ID" value="MESCA003802"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_981036_0_0_1"/>
    <dbReference type="OMA" id="ICDHTIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="104"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003802-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="284" mass="33603" checksum="CC1334B9832BE749" modified="2013-10-16" version="1">MQTKTKFAKSRQSTDTASVEPQIDDLNDQICDHTIRQPCFPEFRNIHQENRVPEIANLMNQVRKWDFSFNGTGNGYTAVNFLERIEEHAVNYQIPPHHLITVLSVLVKGEAEDWYRCNKHMWLSWEDCKEHFKLFFVPARTRAQMVDDVRAYKQGQNQPFKKYVIAMQKLMRYVPEMSEEEQLDRIYRNLGKEFTLYIKRSEFSSISELMRLGEEHEEKLDEDKRSKYNNGKQNNMYHAQKNSAISYQNTYLENGDGQFRDKNSFLVCKKLVGIILSEIIPEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKV7</accession>
    <name>T1GKV7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKV7"/>
    <dbReference type="STRING" id="36166.T1GKV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004138-RA">
        <property type="protein sequence ID" value="MESCA004138-PA"/>
        <property type="gene ID" value="MESCA004138"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_615805_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004138-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="445" mass="49156" checksum="AE49C8D7D7AF18CD" modified="2013-10-16" version="1">MNGVKYDQKHNHHQVSHDHHGNQYEQHQQHYHQEQRDQHGQKLIKTDYDYQRKNNQVVIQNEEVKTGYDHGYQPQPQPEKKISTPSKRVHIKLPEFDYSSYTKTDSEIKYDSRPVVVDKPTSKEPRKYQKTKQQYVQKPKPQNRYEHIQRIPNDSPRSQRPRIKARIQKPSHTYRQRPQTQISLPQDDYKRLKGKYRKYHLKAYDEDKDHVLYYVDQPVQIYDPQEKKFHQFDKLSDEEAEEIQKKTSEYKGVVVSGVVLGHESQIHAGQLHISGCGFGVTMLSSLGQGGQVQTGQVHQIGVDVVVDSSVVVVSGVEGVTSGVGVVISDVVEEVTSGVEGVVISTVDDVISGVISGVEGVISGVISGVEGVTSGVEGVVISAVDGVISGVVFGVEGVISGVVISGVDGIISVVTLGVEGVISGVTSGVCGSSVVRGLLVARCFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKV9</accession>
    <name>T1GKV9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02114897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02114898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKV9"/>
    <dbReference type="STRING" id="36166.T1GKV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004140-RA">
        <property type="protein sequence ID" value="MESCA004140-PA"/>
        <property type="gene ID" value="MESCA004140"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040373">
        <property type="entry name" value="CASZ1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451">
        <property type="entry name" value="TRANSCRIPTION FACTOR CASTOR PROTEIN MING -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN CASTOR HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004140-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20526" checksum="76753720000D8E98" modified="2013-10-16" version="1">MHSNYHRKDTAIIMEGFQRFRSSEEPPPRKREEIIRHLKWHKKRIESINFGFMRFSASDDCSRSFPAPPPLFKDQKTTHFHCIRDTCKFTFKNKADIVVAHRKHHEKHDSVKTSGFIKISQNESCFLNEDNDRESEEGGNPAECVHSLKQTHYHCETCSGVVLSKSLIPIHRHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKW1</accession>
    <name>T1GKW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKW1"/>
    <dbReference type="STRING" id="36166.T1GKW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004142-RA">
        <property type="protein sequence ID" value="MESCA004142-PA"/>
        <property type="gene ID" value="MESCA004142"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1121194_0_0_1"/>
    <dbReference type="OMA" id="GTDACQM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF65">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="61"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004142-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="248" mass="27617" checksum="47B84CD02E9BCADB" modified="2013-10-16" version="1">MIQDEDAITVQEAMKRSDSDKWEEGMKSEMNSLDENKTSFQLIFGVALCSAATYNQPSARIVNGYDARTDSTPFMVSIMRFGYHSCGGSLINHRTVLTAAHCLINTSSSQLKVRSGHCSNSNVQSKRYSEAWELGTIYGWGIQHQNYLKSPIHLQYAKIPVIDTMLCQQMMLNRISGTMFCAGYIKGGVDACQNDSGGPFIINNKLYGIVSWGNGCAMPGRPGVYTNVSVVRPWIDEVLRKNYNEKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKZ8</accession>
    <name>T1GKZ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004181-RA">
        <property type="protein sequence ID" value="MESCA004181-PA"/>
        <property type="gene ID" value="MESCA004181"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963452_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004181-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6576" checksum="1EAB56F1030FA66F" modified="2013-10-16" version="1">MEKKYAEYEMVCTSKSGRLAALKIISGYRTISGGAVYVLAGRKAQPLVSFEDYTSCRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLE0</accession>
    <name>T1GLE0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004340-RA">
        <property type="protein sequence ID" value="MESCA004340-PA"/>
        <property type="gene ID" value="MESCA004340"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433191_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4090">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004340-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10937" checksum="A0766C499798704B" modified="2013-10-16" version="1">MFLSNRLDKKSSRGMIYENEELRLRTININAEVAEGQSSIRKLRSENDRLKREIWSLKDNYDKLKKKYKKREEIKYAEHHHSTCASYSNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLE5</accession>
    <name>T1GLE5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLE5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004346-RA">
        <property type="protein sequence ID" value="MESCA004346-PA"/>
        <property type="gene ID" value="MESCA004346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888317_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7669" checksum="56EBDAC3543A2F1E" modified="2013-10-16" version="1">MLNILQSKILAFRIQKFPHSKYSDLHMAKEKKIKEFPIWPHTSWSDLLPQKCYFMIYNSIQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLH8</accession>
    <name>T1GLH8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02022375">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLH8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004380-RA">
        <property type="protein sequence ID" value="MESCA004380-PA"/>
        <property type="gene ID" value="MESCA004380"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239690_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004380-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11925" checksum="D6FA5F7C00B760DD" modified="2013-10-16" version="1">MKWLSGCCTLFATGSYTPFLRSRPTFSFLIDGRKSSTDQIFKIGKSSKSPRVLRKSTTVRIEMNEFGMSDIKESGSQTRLEESSPEIEDRHHSAARDQMEKKRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM96</accession>
    <name>T1GM96_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390002">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM96"/>
    <dbReference type="STRING" id="36166.T1GM96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004665-RA">
        <property type="protein sequence ID" value="MESCA004665-PA"/>
        <property type="gene ID" value="MESCA004665"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_25_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004665-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12530" checksum="6C3597DDAA0535AE" modified="2013-10-16" version="1">MLFLFKVDQLTQDMTANNFTVSAMHGDMEQRDREVIMKQFRSGSSLINFDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDKRTLKDIEQFYHTTIDEMPANIADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN64</accession>
    <name>T1GN64_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02002691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005007-RA">
        <property type="protein sequence ID" value="MESCA005007-PA"/>
        <property type="gene ID" value="MESCA005007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624839_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9157" checksum="31FC8EAA6E48D28B" modified="2013-10-16" version="1">MCPITIHRIWDNMKEEGVNLQNVNLLTSEELKTFFPKLGPRVEFRNLITEKNNEKRAKRISDNEKVSSVPRTQFMGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN75</accession>
    <name>T1GN75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN75"/>
    <dbReference type="STRING" id="36166.T1GN75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005019-RA">
        <property type="protein sequence ID" value="MESCA005019-PA"/>
        <property type="gene ID" value="MESCA005019"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123514_0_0_1"/>
    <dbReference type="OMA" id="PENEAMH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005019-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14479" checksum="1FE513F14F3E6392" modified="2013-10-16" version="1">DPINLAVLSLKENGELLKLRNKWWYDKTECNLNHDAQETTHSELSLSNVAGIFYILIGGLMVALLVAIIEFCTKTKDPPSNRNSIDTMHSKSKLTIQSTRDFDNGRAYYPSLSASPENEAMHMNTHGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNS9</accession>
    <name>T1GNS9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02145311">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA005239-RA">
        <property type="protein sequence ID" value="MESCA005239-PA"/>
        <property type="gene ID" value="MESCA005239"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203571_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005239-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12689" checksum="53C44A6ECD2C04D0" modified="2013-10-16" version="1">QYLTFQSASEHRLRSITDSLPAPCGLISSVIVWSIVPEHTLFENFEIDVCNGMDCAMRWKKIFGKKMFLYKPELISXCRGWKKMFRHENVSLQTRIDIERKEDLEPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP31</accession>
    <name>T1GP31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NIF3-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP31"/>
    <dbReference type="STRING" id="36166.T1GP31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005347-RA">
        <property type="protein sequence ID" value="MESCA005347-PA"/>
        <property type="gene ID" value="MESCA005347"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140434_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005347-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="19822" checksum="ED60E05BEF70B7F6" modified="2013-10-16" version="1">MKLKKKCAMNLNSYIFPYLFNLIYYQSSSVEITLPEVPASVGGTGRILSIDRDLTLRDAIEKVKLHAGIKSVNVAIGNNQNIDSSLIKTVGICAGSGASVFKGVKADLYLTGEMSHHEVLDAQERNTSVILCNHSNSERGYLKEFKEIFHKQVPENEVKVIVSEVDSDPLESKTSFRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ39</accession>
    <name>T1GQ39_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ39"/>
    <dbReference type="STRING" id="36166.T1GQ39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005738-RA">
        <property type="protein sequence ID" value="MESCA005738-PA"/>
        <property type="gene ID" value="MESCA005738"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_312917_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005738-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="937" mass="104001" checksum="57C8D312EA81EA51" modified="2013-10-16" version="1">MENEKSQWPIVHVSLMKSDSNSSLIEGFNPISVSEHNSEIKEFSMESNGSLSESSQSEITDKHSTENSEENKTTYEDNDHPYYPSFDESDDFIQEEHTSIKNSQNIPYVTVNRKGASNNEPHFVEKNRNHEENYVKNSENISENSDMNIINSQNKAYVTLVRGHSESSKNEGSSGDSTTARVKEVDTDSQDSQSMSHDSVNSETHEDSTKNSNVINSQNKVYVALVRGHSENSSKEHEGSGNVSSNIEGTIEGHVSSMTQTNRSQGNDTFSSNIVNSQNKNYESTNRNHETESEASNEIYGHEDSNNNVVSSTIYKYHETNQGTVSDGNENTYIIQEKNSQKITNPEDISEEHKVISQMNTILVSPSNASKDFAEAMSHHSDSTKSEEHKIISEMNRFLEIPSESIESNIQLTTRIVNPSNSSREYAESISHHINTSQNSGTTFQESSTGQVAIETGSSVLGNESQKSSTEHRTTENVIKSFVNVYDIESQDSSTEQGTTESSTVTSTEGYIQVFNSEHQESSTKQEVKETSTGSSLNVYKSETHESDQYRKETENRGLVNVLSTDFQEVSTSINSESHEPSNEQKIIEGSTKESIQILNVESHESSNGQERIESSTRTTVNIYDSDSQQASTKQGLTETATKSSINEDLADCGFIVSKKFHEWRAQYNYERNQKSQANISSKGKNEEFSPVKQLGTFLRRMSSLIKGKFEEADDYELGIIHSKLSASQKFSSRYRSDSLSYSLSKTERGKQAYTPTVPPYVPLESKNQPVHIYPEKTMQMVYDEAAGVWNMVEIIPNGLTRSEIIVDTGDGRTQELDDLQGASATISIYSALADILTKNAPTTTTTTTTTTSTTTELPEIIKVSTTSSTADNPVLLDILKKNYIRGNGKGSKSVFSLTTAKSAIDSSTVSSNDVFDEIFGNEIISRKYLIDDGQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ80</accession>
    <name>T1GQ80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005783-RA">
        <property type="protein sequence ID" value="MESCA005783-PA"/>
        <property type="gene ID" value="MESCA005783"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186947_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005783-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12854" checksum="D65198EAA4E97DCB" modified="2013-10-16" version="1">MRKLVPNRIRVPLENSKTLWRNRHCSIATLQREEMENMKSLISGSLFFNSLQFDCSKILEGNRHYVRLQPRRRKMRGGNSNRKCKRRRIKCGEGEDATPKQQLATKVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD4</accession>
    <name>T1GQD4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005838-RA">
        <property type="protein sequence ID" value="MESCA005838-PA"/>
        <property type="gene ID" value="MESCA005838"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796877_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005838-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7675" checksum="F4A331E6791732C2" modified="2013-10-16" version="1">MYLLVYTKPYVCYESLGYAKPLGLTAHIAAACYTFHYPTFLWIDSLIQRCHCPTSSRTAHSMGDPPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS0</accession>
    <name>T1GQS0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005984-RA">
        <property type="protein sequence ID" value="MESCA005984-PA"/>
        <property type="gene ID" value="MESCA005984"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471648_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005984-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9478" checksum="E6C737B72F9DC43D" modified="2013-10-16" version="1">MLMISLSDDGCKDLVLSSSAGMPFVPALLWFLSLWIALSTSGRFGGGTSSLPNECYIFQCEVFAILKAAETTMFGAAETTSSRHFLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQY5</accession>
    <name>T1GQY5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02099874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GQY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006051-RA">
        <property type="protein sequence ID" value="MESCA006051-PA"/>
        <property type="gene ID" value="MESCA006051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_9_4_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="334"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="460" mass="50241" checksum="D78BD01E57FBE497" modified="2013-10-16" version="1">AAVHVIVSQLKDLIGVPLTKNTSPFKLVYTVYYFCEALPKANPAVVIFSLCVIVFMAICNELIKPWLSKKCRFPIPAELLAVVTGTLLCRYLDLKTRYNIATVGSIPPGLPDPSFPPINLFPMVFVDSIAIAIVSYSIVVSMGLIFAKKHSYEIFPNQELFAMGISNIVGGCFQCIPIACSLSRSMMQDQTGGVTQMASLISAGLILSLLLWCGAFFETLPRCVLAGVIVVALKSMLMQAKALKKFSQQGKLELLTWIVTFFGTVFXXXXXXXXXXXXXXXVTFFGTVFFDIIYGLLSGVCVSLLSVYIKGLKPSSSMLGVISLGDAIYVNLQHHKSAKEVPNTKIFHYNGSLNFATKNSFKKTASFVAVTDASKPLTETAFRSLILDFSALGHLDVAGCGVLNDIRNDLKLLDVRVFIASPCDKVYNSLIHYVALGQESFEIFPTLHDAVLYSNTCLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR51</accession>
    <name>T1GR51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02156621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GR51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006124-RA">
        <property type="protein sequence ID" value="MESCA006124-PA"/>
        <property type="gene ID" value="MESCA006124"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_797628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577571" description="Carboxylesterase type B domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="3">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="3">
        <location>
            <begin position="123"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="348" mass="39170" checksum="B11DE1BA5898089F" modified="2013-10-16" version="1">MCQTRQIYVFSLILIVISNAFASDLRVELKHGASLIGINLKSYKGRQIRSFLGIPYAKPPIGDLRFKPPEPFTWEGERNATDDGTQCFQTYFFDPTGQAKGSEDCLFLNIFTPPLYRIPKXWNSHPFFVFQPVLEPTHLGAFIGVDPRKEDLNSQNIPILTGLTSGEGLMATASILMKEELLNDLKSDVSNKFPLTLGYDHWDLEKQKDITNKLEKFYLKDGHNYDKNNHQNFTDIYTDSLFLAGLDQYLRKRLSMKSAPTFVYIFDMAHNHSTTKMLGGGDIYLGVNHGDDLRFLFPFGSFFGGKSHLDGNSLAMKDALVELLVNFAAYGSSQAPTLRAIQNTSGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRQ1</accession>
    <name>T1GRQ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006339-RA">
        <property type="protein sequence ID" value="MESCA006339-PA"/>
        <property type="gene ID" value="MESCA006339"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1628946_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006339-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18818" checksum="5F26281EA1C8FF45" modified="2013-10-16" version="1">MKCFSNAVLKKIRSKLIWTSINTIFFAFLTERVGASDPVRVDEETELFFDDPTMEEQPLRRSEFCIYDVTGSYNLEISKTTNYCIFGDVECCSGMVWLNGLKDEIILESPKIILNTDNIGALQLTKYTQGLSTLMQWNNFDELRWNKSHDSRYLNKSATKAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS80</accession>
    <name>T1GS80_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02174880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS80"/>
    <dbReference type="STRING" id="36166.T1GS80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006530-RA">
        <property type="protein sequence ID" value="MESCA006530-PA"/>
        <property type="gene ID" value="MESCA006530"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2678094_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF1">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DAF-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006530-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8664" checksum="6D1277AB102D6C9A" modified="2013-10-16" version="1">MSSEKIVPPPVKWAQRTDLLYVDIAAECKDIDFKFTEDSMNFKGVDSSSNQKYEVTLNFYNKINPDNILTKNNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSA8</accession>
    <name>T1GSA8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSA8"/>
    <dbReference type="STRING" id="36166.T1GSA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006560-RA">
        <property type="protein sequence ID" value="MESCA006560-PA"/>
        <property type="gene ID" value="MESCA006560"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906650_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006560-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6915" checksum="6547423B8EEA40A9" modified="2013-10-16" version="1">MHQLQRPTYIIRFIIPPLIDMVSELGIFGVVIGAGHMLRSKISKPNEDGVTIGICELWTVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT72</accession>
    <name>T1GT72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="CAQQ02148083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GT72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006895-RA">
        <property type="protein sequence ID" value="MESCA006895-PA"/>
        <property type="gene ID" value="MESCA006895"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001103_10_1_1"/>
    <dbReference type="OMA" id="IMCKVIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18018">
        <property type="entry name" value="DEXHc_RecQ4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000772">
        <property type="entry name" value="ATP-dependent DNA helicase Q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF108">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="660"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="855"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="130"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006895-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1342" mass="152296" checksum="2B7750AB7FF95815" modified="2013-10-16" version="1">MFGVFTICWKFRAQFNIKVYEMDDQEFKKKYQKYKHKIKIWEHSFKKKNGRIPSKLDIHDAPMDIRQSYKLYFKMKSQLLDKTLGDIFDDDGFNILENSNEIDTSMEDDFPDINNITNIDEGHVIKEFVPKETSKQNETAWGQTLTKKVPVKPKKENSPIKSNSTFKPSGSLFKSQSFSKRNPRKSLMRQSSEASQGNFSQTSADSQDVLPDLETILSQKAKEKLESASQSVTLSIPQNLQMSNQISKDIDIGWLNRISEENGVLIKTDSNISGKKSTFGLSNIDLNKIKKIPETEEISKKIDVFQKVDMIGEIEPDLEIVSNGEDEVVAESDNEEGTYRQAAKRRRILPQKSETHSIVEKENKPNEDEDEILKANTLKLDLSTKPVKKRKRKFSESSTENYISGSDSDFDSKKSQRLKLERLVQKRVKAVKPIPVKKPRRIARVKRTVQSEITETEDKPEERILPLDVMDVKVCPRMQLEDLKQIDVIASSYVSKVVDLTTIPKKSSQTGPIENDKSKAARSKLEERIKSGKLNENFVTMNLRKKLFEKNESDSDIDNSGNNETDHNIDQEDLLAGIDSDEFDTGFNQEELIEVTKAQLVPEDFLKKAGLLEVAENNANIGPVYDLSEDGCVREPTEEVYEVLKMFGHTSFRKGQEKAVMRILSGQSTLVTLSTGSGKSLCYQLPAYLYRKHRKSITLVISPLVSLMEDQVHGIPNFLRAHCLHTGQTSQKQLTILEMIQRGEIDILLLSPEAVVAGEKSTGFGSILRKLPPIAFACIDEVHCVSQWSHNFRPSYLMICNFKGNTILGLTATATLPTRTSIIKHLKIHDGENGIVSDIPLPDNLILSVSKDVRKDDALLNLLRSERFSCCTSIIIYCTRRDECEKVASTLRSMLPQAEQPESTNKKRKRTIYQAEAYHAGLPSSKRQAIQNRFMRNELKIVVATIAFGMGINKPDIRGVIHYNMPKNFESYVQEIGRAGRDDHNKSDQNELRRHIFANSIDRHVIRKLLSKIFIPCSCVKNFKKSEFVSQHQDVIKSKSDNIELGPRNHVCPGHEVGFSVEETVQELDIPSENISTLLCYLELDSRWCLNVLSNAYIMCKVISYGGPKYLKQSAKTCPPLAAAIALKVKNGTFDETSNIIEFSVIDISAAIGWNSGVVKYQLKNLEWITNENGFPKRSPISVTYFDLGFRVKSPGDFTYEECEEALISLHERSVGQEQAQLKQLQYVYKGLSNVCYPSCLACLIVDDTLIEKCNSLKNTIRSYFKNDCKDEIIIQPTLEEQTINEISNNVRSLINMYPENRFTGRAIARIFHGIQVQSIQQLFGVDVNSGERIWNMILIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ9</accession>
    <name>T1GTQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02018150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ9"/>
    <dbReference type="STRING" id="36166.T1GTQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007100-RA">
        <property type="protein sequence ID" value="MESCA007100-PA"/>
        <property type="gene ID" value="MESCA007100"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002173_2_1_1"/>
    <dbReference type="OMA" id="EKHYYFI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000011">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="710"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007100-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1057" mass="121712" checksum="0D3B29E2E13AFA80" modified="2013-10-16" version="1">MYTSFDGDYRRRPMQSLGGHSMSTNRDSIIKKSQEQRQKRSELRKQTASATQIQSYIRSFLTRKHTKSSERTNFDEFLRLRKNNLEANFDANEISYVLQRFVFFYDSGQDSDRLIFICKILVKHTKKIVELLEACPQWRYRLREICNICLQQLSTSSSGSHAIPMRIIEIYTDIKYSSSKQILLQLVEQRYFDKVRNILELKCPPLDGETIRPPDPTTEAVFDMIMRPLAFVAADTYSDICLKFSECFLSQKPSESISCFLIPTLSQPEQSLKFPFIKIIEALGEILPNSSDDCKMEVDLNLKNLDRNLDLKDFSGYNVCSSYLGIMSHLSQHLLKLPKSAVKTKLLSLGALKFDMDDSSKDEYDSDSDSEETPTKNNDGDFFETGVLLDVVEFLYKPNRINWIFQHQIENFSDNPKQLYQISVFCHNFLLMSNFSTASNIELFNISNIPKFLRSLWFTLVNQSNQLGFNSPLALISKGVIPKADTDIERTIPFLATFCAIYSKRLPMMHDDDFSSKIDENPLPFSLNEIIHLCKILKEISLGLVELAFPETRTNLNNHYRNVLGHTEADNERIKQKQQVWAHLLNVVVCVINQLYNRDLRMNFCPEGAWVLENLNLTLDKDWDVPLVRARRGHRPFEPIKDFTRKDFDNGPPPSTKQLRSVTILREIPFVVSFARRVNILQGLIASDKMKIQGGLHAFLQGPAIHLSVRRSHLYEDSFDKLRPENGLEEIGIDGGGVFREYINELLKTAFDPNRGFFMLTTDNKLYPNPNVADLVENYEKHYEFIGRILGKAIYENLRVELPLAECFLTKLAGRKYSTHDMHQLASLDPELYKHLLSLKSHKDIDTLGLDFSIVTSSFGNTQVKVHELKPGGQNILVSSHNINEYIGLVADFKLSKQIHKQCKAFRRGLSNLLPIEWLNMFSNKELQILISGTEIPIDLEDLKNNCRYGADYSPDHPTIKCFWRVVEEFDEDQKRQLLKFVTSSTRPPLNGFKNFYLFPPQDLEPPFSIQNTNDEDRLPTASTCTNLLKLPPFKNDETMREKLLYAIESGAGFELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUA9</accession>
    <name>T1GUA9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02031875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUA9"/>
    <dbReference type="STRING" id="36166.T1GUA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007319-RA">
        <property type="protein sequence ID" value="MESCA007319-PA"/>
        <property type="gene ID" value="MESCA007319"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020502_0_0_1"/>
    <dbReference type="OMA" id="NISSFWW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007319-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="38212" checksum="4B15D81691FA45BC" modified="2013-10-16" version="1">MCLISVNVSDWLGGDGREPSVPFLTAVFFGLNFLAATQDIAVDGWALTMLKRCNVGYASTCNSVGQTAGYFLGYVVFIALESKDFCNSYLRSVPKEEGLVTLPDFLWMWGIIFLVTTTLVALFKKETEEKASEDNSRFTDHPDLDIHESYKVLFNIIKMKPILILSAILLTVKVTFAASDAVTSLKLIDAGVPKEKLALLVIPLVPLQIILPLVVSRYTNGPKPMDVYTRAIPYRMVFSAFAVIITYFTPRMIDAASGHVPFYYYVLLVGNYALYQIFLYSMFVAVMAFFAKISDPAVGGTYMTLLNTVCNLGGNCQTLWFYGWLTHFPGKSACIQTKLKKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUL2</accession>
    <name>T1GUL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S15a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02068272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUL2"/>
    <dbReference type="STRING" id="36166.T1GUL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007427-RA">
        <property type="protein sequence ID" value="MESCA007427-PA"/>
        <property type="gene ID" value="MESCA007427"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_1"/>
    <dbReference type="OMA" id="EWANNIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007427-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17737" checksum="BB5D6169A3FEE5A4" modified="2013-10-16" version="1">INVLANALKYINNAEKSGKRQVLLRPGSKVIINFLTLMTKHGYIGKFEFVEDHRLGKILLISPRFDVPISIDKWTNNLLPFRQCGYVLEMKHGYIGEFESVDDHRSGEIMVISPRFDVPINIALLPSPQGGYVVLTTCGDIINNEEDRRKNLGGKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUQ9</accession>
    <name>T1GUQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exportin-5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUQ9"/>
    <dbReference type="STRING" id="36166.T1GUQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007477-RA">
        <property type="protein sequence ID" value="MESCA007477-PA"/>
        <property type="gene ID" value="MESCA007477"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002828_1_0_1"/>
    <dbReference type="OMA" id="WNDSTIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="1">
        <location>
            <begin position="29"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007477-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="739" mass="82697" checksum="B9A90ADD70C7A038" modified="2013-10-16" version="1">MNSGMREIGVSVNLDLRFWKRNHICSTQDPAYLDWEALVAVLDGVLSRILMVTERPSVQTGLRLLEECLKIDSQDPLIISILLSCISSLFVFLSMSSCQITSGNVVAMSGVSLLPRVLEKIFSALVYNNNNSTNVEAVKNLRRHAASLMVKLGHKYPLLLLPVFDQINANIKSLTRPICQLTKMEEITLQEALLLISNHFCDFERQSAFISEIINPAHAQWMTLTNVFKSPIDFMSFIGLDQPGVDPNLNDPYKEHRKQLSDALYIVLGVIKRCTWPEDPDRASRGGFVVALTESGNPICRILQPIIVLNELFTPEAIQRLSNSFKKVHGMLEHEKKNLMGIPPIFNDPMDPNAKKPATALDKMQTFLTTLFDNCYHLMGSSGPSLGRDLYQLSGIEDALINSAFASLNHVPDFRLRTIIRVFLKPFVYSCPPAFYEKVLLPIFKHLSPFMLDRLTTRWNYIASLYESGELNEEVNDTQEVLEDMLNRTLTREYLDVLKVALVGGSLTIETNGGGTTDVTMDQDEHSMDGPSHGLSRATQNALTSEVISDLGGKLLKDPVTCSPIVMTILNLKSVFLTGPIIRYLTNEQLITQQIASATLISVLQGLQAHGQHDSNQSVLITLGVQIYEILRPKFPALIEIMQQIPNTNIADLQKLDEKIAMGHQTKGNKIDKAKKDLFKKISAQLIGRSMNQLFRQEVQIANLPPINLPPRNRNIDLIENSQEAGINKLFTNPTKEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUU6</accession>
    <name>T1GUU6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GUU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007517-RA">
        <property type="protein sequence ID" value="MESCA007517-PA"/>
        <property type="gene ID" value="MESCA007517"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_245232_0_0_1"/>
    <dbReference type="OMA" id="NRMTRIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20953">
        <property type="entry name" value="IgI_2_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20956">
        <property type="entry name" value="IgI_4_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20958">
        <property type="entry name" value="IgI_5_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000017">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000302">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000308">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000324">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF53">
        <property type="entry name" value="DOWN SYNDROME CELL ADHESION MOLECULE 1, ISOFORM BQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="103"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="189"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="288"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="440"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="543"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="718"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="815"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="912"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1009"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1114"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1152"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1248"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1388"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1484"/>
            <end position="1575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007517-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1578" mass="171515" checksum="33488A3C1C33674F" modified="2013-10-16" version="1">PVGSVAPKISFDNQFKQAAVGLSESISIFCPAQSYPVPAFRWYKFIEGTTRKQAVVLNDRVKQVSGTLIIKDAVVEDSGKYLCVVNNSVGGESVETVLTVTAPLSAKIDPPSQVVDFGRPAIFTCQFSGNPIKTISWFKDGKDIKHKEAIFKIESVKKEDKGMYQCFIRNDQESAEASAELKLGGRFDPPVIRHKFQEETMQPGPSVFLKCTAGGNPTPEISWELDDKKIINNEHFQVGQYVTVNGDVVSYLNITSVHANDGGLYKCIASSKVGTAEHSARLNVYGLPYIRQMDKKAIVAGETLIVTCPVAGYPIDSIVWERDNRPLPINRKQKVFPNGTLIIENVERNSDQASYTCVAKNAEGYSARKALEVQVMVPPQISAFSFGDEPSNNGDMAGVQCMVSKGDLPLNIHWTLNNETLTSGDYDISISRLNQRTMAPSISPFHFGDTPLNREEFASVTCVVPQGDLPLDIYWTLNNALIINGENGFSLLRLNKRASILSIDSLEAQHRGIYKCIANNSAGFSEFSKELTRINMCLSIVPPQIIDFSFGDEPANTGEMATVTCLVPKGDLPVNIYWNFNNKTINSGDHDISIARLNPRTSLLSIASLEGVHRGTFTCIANNKAGFTATSSVLHVNGDLPLNIYWNLNNRTINNGYHEISIARLNPRTSLLSITSLEGVHRGTFSCIANNKAGFTEISAILHVNVVSLNMCLSKVPPQIVEFSFGDEPSNIGEMASIQCLVPKGDIPLNINWNLNNRSIHNGLIDIKILRLNPRTSLLSIESLEGSHRGTYTCIANNKAGFTEYSAVLKVNVAPVITHFTFGDSPLNRGEMASVTCLVPQGDLPLDIFWALNSALIVNGEDGFSVLRINKRTSILNIDSLESYHRGVYKCIANNSAGYSEFMSELKVNVLPKIVPFSYEDTIHVGESIDLLCQVSKGDQPMQITWTFESNKSSYDRQPSITTHRISKTASALNIPSATEYHSGIYICTVSNHAGKTVYNTSVTVNVPPKIAPFSFGEFPVSFGDVVSVQCTIAGGDLPVDVKWLINGEEIASYLEIMLDKRGKRIHVLTIESVSGKHAGNYTCMAFNNAGLVNQTSELKVNGINVVKGGKKASMLTIESVNGRHAGNYTCTAKNSAGSESYSTNLSVNVQPKIAPFDFGDVPANFEDSSSVTCLVLSGDLPIDIQWLFNDYPINSFSGISILKAGKKGSMLMIESVNERHAGNFTCRASNSAGSVSHSAQLIVNVKPKISPFDFGEVPANFEDSSSVTCLVLSGDLPVDIQWLFNDYPINSYSGITIGKMGKKGSVLMIESVNGRHAGNYTCQVSNSAGSETHSATLVVNVLRNGKKASVLTIESVNGRHAGNFTCRAKNFAGSSTYSAMLVVNVPPKIAHFDFGEEPANFGDSASVMCLILSGDLPVDIEWLFNDYPVNSYMGVSIMKGSKKNSILTIESVTGRHAGNYTCTARNSANSVSQSAELIVNVPPKIAHFDFGDEPANFGDSASLMCLVLSGDLPIDIEWLFNDYPVNSYMGISIMRGSKKNSMLTIESVNGRHAGNYTCIARNSANSVSQSAELIVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUY7</accession>
    <name>T1GUY7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUY7"/>
    <dbReference type="STRING" id="36166.T1GUY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007560-RA">
        <property type="protein sequence ID" value="MESCA007560-PA"/>
        <property type="gene ID" value="MESCA007560"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109341_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007560-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="251" mass="27664" checksum="2540CB386C8FC55F" modified="2013-10-16" version="1">FFRKDHLAEHFTTHTKTLPFHCPVCNRGFQRQIAMRAHFQNEHVGQHDLVKTCPMCSYRAGTMKSLRVHFFNRHGIDLDNPGARSPPSLLLALSTNSKTPFLPSTVAAAAAAAAAASSSSAGATVTYSDSGESFRSVDNTTPPLNLLTPQVEILTTDTEKSLKAMSCNDELQISPVEPKESNIPTDYSPNSPYGDGNSSSNHINEKIPIDDKNSIKPLISLFQLKMRMLEMNLIIKWILVIVSLGTHQSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVH4</accession>
    <name>T1GVH4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007778-RA">
        <property type="protein sequence ID" value="MESCA007778-PA"/>
        <property type="gene ID" value="MESCA007778"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870149_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007778-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7253" checksum="C2FC8E8052DBD223" modified="2013-10-16" version="1">MYTSKVSPLRKFPTLFGGGFDSTSFFCFLDNQLFNQERSQNQLAPKVIIGDCEQFQKSPLTTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVM4</accession>
    <name>T1GVM4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CLIP domain-containing serine protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02025091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVM4"/>
    <dbReference type="STRING" id="36166.T1GVM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007835-RA">
        <property type="protein sequence ID" value="MESCA007835-PA"/>
        <property type="gene ID" value="MESCA007835"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725114_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007835-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="7732" checksum="614ABBE16CA59888" modified="2013-10-16" version="1">MFLQNSQCENGFGRMPYVCCTEDTGYTNTQQPTPTTRPVTPSVPSTPGDGKGLQLPSPPCQIPYSLALNVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW03</accession>
    <name>T1GW03_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdopterin synthase sulfur carrier subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW03"/>
    <dbReference type="STRING" id="36166.T1GW03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007971-RA">
        <property type="protein sequence ID" value="MESCA007971-PA"/>
        <property type="gene ID" value="MESCA007971"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_4_3_1"/>
    <dbReference type="OMA" id="HVLFFAK"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990133">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044672">
        <property type="entry name" value="MOCS2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359:SF1">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007971-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8152" checksum="008D0C97AE9103B2" modified="2013-10-16" version="1">KAREYAGTPKAELSLPKELKAADLLNFICAEYNLENFKNTLILALNEDYVEDSEELVLLEEGDELAVIPPISGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW10</accession>
    <name>T1GW10_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase 1-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW10"/>
    <dbReference type="STRING" id="36166.T1GW10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007978-RA">
        <property type="protein sequence ID" value="MESCA007978-PA"/>
        <property type="gene ID" value="MESCA007978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_2_1_1"/>
    <dbReference type="OMA" id="PKLILYG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF4">
        <property type="entry name" value="FI01423P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="90"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24576" checksum="3AFC248AD52DE8D9" modified="2013-10-16" version="1">MGSKPILYGFDLSPPVRAVLLTAKALDLDFEYKQVNTYLGENKTAEYLKKNPQHTVPTLEDEGRCIWDSHAICAYLAEKYGSTDSLYPTELMKRAHVDQRLHFDSGTLFTCLKSVALALRTGQPITDDIMTSVYQAYEFLKLFLANSLYLVGDLVTIADFSCVSTLSSLDIFLPVDGEKFPKIKGWFERLKILPYYEEANGRGIQMAIEMFKDKMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW43</accession>
    <name>T1GW43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02068311">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW43"/>
    <dbReference type="STRING" id="36166.T1GW43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008014-RA">
        <property type="protein sequence ID" value="MESCA008014-PA"/>
        <property type="gene ID" value="MESCA008014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662795_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF9">
        <property type="entry name" value="LEUCOKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18704" checksum="1C86B032A2A0EA33" modified="2013-10-16" version="1">MTIFVDYELIATKRIEVADFAGDIETRLYSSGYEEEFQLEYVSYNQELPDMITCIYEDMTQCIEFCIPGSKEVIKMLIIVVIVFGVCWLPLQAYNIIYVTIPEINEFHYISIIWFTCDWLAMSNSCYNPFIYGIYNTRMLNIQSLPKTIFSDNFVVVYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWJ9</accession>
    <name>T1GWJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02158329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWJ9"/>
    <dbReference type="STRING" id="36166.T1GWJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008176-RA">
        <property type="protein sequence ID" value="MESCA008176-PA"/>
        <property type="gene ID" value="MESCA008176"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369284_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008176-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23077" checksum="147C979E6AC7AAEE" modified="2013-10-16" version="1">MMTAGQTIFTEITAGNYRIGLVTLYDTFWSVGLFFLPTLSSFFTSWGQIYLGISFPTIILIVSLIWIPEAPRWLLKNKNDIKEVEAIILRAAEINDRKYLLPSNLRDELHLQATSLKSQPPQAKWLDLWKGPRAKTHFIASHMALAFYIINFMGMLLNTRSFGRDYLAVNTIFTGFSEVIGCFMAMYFSIHLKEKKWQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWQ6</accession>
    <name>T1GWQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008235-RA">
        <property type="protein sequence ID" value="MESCA008235-PA"/>
        <property type="gene ID" value="MESCA008235"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2216486_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12091" checksum="D793B125B27E5037" modified="2013-10-16" version="1">MTTLYHLWFDYKNGRHLYIIAQKQKYSKVVLKVGSKMRQNTRIFAHVAILIVVATFTKSQDINCNQPPPMVDPHTCCQDGGRDEVADVCAEKWGLKGNPPPDMETAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX64</accession>
    <name>T1GX64_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX64"/>
    <dbReference type="STRING" id="36166.T1GX64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008406-RA">
        <property type="protein sequence ID" value="MESCA008406-PA"/>
        <property type="gene ID" value="MESCA008406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034504_2_0_1"/>
    <dbReference type="OMA" id="DWMENDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="34042" checksum="5D926BB82A656DF3" modified="2013-10-16" version="1">MEVDDHQFEETEEIYQRSLEDVHEILKHAKVDEKTLTPLTQAIYYPDEELPDLRLVELDAHILQQIHEGSNMYFKGGLDEKVVLCTDEKTYEVKTAEISNCLLVIPDLKYAQQTSKSPLKSPKNQKGNSSFNNSLNDSTEEENMNRSLESKKVQKIFNEYFELKEIKPRYKKIGDLLQMTRYSGPENEFCIDHKLLFTYDELFDTIQCSRKEFNEGLRFYRAIELDGFFRIMDYEYEYRIVNHMVQLINENSWSLDHIDRNTTYQSLVGIAPREIVEGLFEIYTKHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXB3</accession>
    <name>T1GXB3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02158565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GXB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008457-RA">
        <property type="protein sequence ID" value="MESCA008457-PA"/>
        <property type="gene ID" value="MESCA008457"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013173_13_2_1"/>
    <dbReference type="OMA" id="YCAMKLP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="176"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008457-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="445" mass="47226" checksum="E304CC949438DD3E" modified="2013-10-16" version="1">DAHNVILNYDDDLKASFFAVYDGHGGAEVAIYCSEKLPTFLKNLDKFKDKNFHEALIEAFIGMDGDILKEETMEVLKKIAGEKNPPILLNPMDEEEDEDEDDEEIGEDENLAELCQESRLPLHEVLLKYGRPKNKIIEKVEDAIEPGTPGGSGLNKKPKPLSPFLRGKRESREISSSEVCSSAAGSSSSATATTSDDSAVSSSSQAPGPSPKKSVENGSAEASPAKSSEKETNEVVSCSPDSSAGKKRNGNTSTEGQSSSADVSSSSSLNKENGEDSVVPTSAAESVENVDSNDHDESSESEDDDFNGEXSSSSSSSSSSSNSTSSPSESEFSISAAAKTYDEEEGFDCCADDPESMIDVPGKDSGCTAVVALIVGKDLYVANAGDSRCVVCRNGKAIEMSLDHKPEDEEETQRIEKAGGKITLDGRVNGGLNLSRAIGDHTYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXE3</accession>
    <name>T1GXE3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008491-RA">
        <property type="protein sequence ID" value="MESCA008491-PA"/>
        <property type="gene ID" value="MESCA008491"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560883_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032004">
        <property type="entry name" value="DUF4790"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16037">
        <property type="entry name" value="DUF4790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008491-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="10055" checksum="5BBBFD6A336CA603" modified="2013-10-16" version="1">MSLKIDSVDIFPFFHSEVKCKLRYDRNCRKYERQFQKELYMLEKYQPSAYSAARFHRHMSITTLWPPLHNIKEIDYAPIVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ9</accession>
    <name>T1GXJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXJ9"/>
    <dbReference type="STRING" id="36166.T1GXJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008551-RA">
        <property type="protein sequence ID" value="MESCA008551-PA"/>
        <property type="gene ID" value="MESCA008551"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098397_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF55">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="74"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008551-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="17083" checksum="386CF4439DE06E0C" modified="2013-10-16" version="1">CYQFGDKQQNHKVQTPNHHSPEPVKDIFPAPIPMDYHSPTEYPLPSFNRFGAYSVFFSRRQRGEKRPIPDEQKDDKYYERRKRNNEAAKKSRDARKIREDRIAFRAAILEQENAILRAQLLAMRDEVSTLRQLISARQAQISVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXW0</accession>
    <name>T1GXW0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008671-RA">
        <property type="protein sequence ID" value="MESCA008671-PA"/>
        <property type="gene ID" value="MESCA008671"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944361_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008671-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6983" checksum="8B4227A412258A16" modified="2013-10-16" version="1">MNSSNCRNGSQCSEQFYSFYVCFPRKRYNNIFIANGPPESIGIDDKCRVLNKHTIHRVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY76</accession>
    <name>T1GY76_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="2">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02185273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY76"/>
    <dbReference type="STRING" id="36166.T1GY76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008798-RA">
        <property type="protein sequence ID" value="MESCA008798-PA"/>
        <property type="gene ID" value="MESCA008798"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590546_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008798-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19497" checksum="C7A793EE7DFAEAD8" modified="2013-10-16" version="1">MDISKDMFKYSKEFVKAGVSLRLIGDQHLLPSDLKEAIEKSIESTSHCKDFILNIAFAYGSRNEMTNAFKIILNQENKSKINKKLIQENLYLNSKPDMVLRSSGESRLSDFLMCQIYDGILFFEKKLWPEIRFMDLTLLEKPTKKVRLSGYGVELYLKSTEYKSQDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU2</accession>
    <name>T1GYU2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009029-RA">
        <property type="protein sequence ID" value="MESCA009029-PA"/>
        <property type="gene ID" value="MESCA009029"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199974_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009029-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12750" checksum="E29D617B17528A65" modified="2013-10-16" version="1">MLWFDITLDKLPKSLDNRFNFYLNILPYLDLVGFWMSVLLGLTCIFYSILQTIFKLRSALENTTDVLQIRLQNHWELKAMNNKPCFRTELEMMPPISSGSEDIPKLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZA9</accession>
    <name>T1GZA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02143806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009207-RA">
        <property type="protein sequence ID" value="MESCA009207-PA"/>
        <property type="gene ID" value="MESCA009207"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1356036_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009207-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="22534" checksum="FCAC700A97A8E50D" modified="2013-10-16" version="1">MIPIIAITIGAVITVVIILIPILYISINCKREISKLPPADIIHDVENNSNLNHHHHQQQLHRQHNNKKCEKDMIIDQSSINEYQTDTGQDTGDYIETTSTTGSTAFKLNSASEKHSPSLNESTKGGTAKNSYFVPLAGPVYIPSDLSKYGSDYHIQESVLTPMKPATQMLENTNPFLQHNDTEDKGGLSIRKKKLKLILMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZM2</accession>
    <name>T1GZM2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZM2"/>
    <dbReference type="STRING" id="36166.T1GZM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009326-RA">
        <property type="protein sequence ID" value="MESCA009326-PA"/>
        <property type="gene ID" value="MESCA009326"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067423_1_0_1"/>
    <dbReference type="OMA" id="EAAHGHK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588618" evidence="2">
        <location>
            <begin position="24"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009326-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="25256" checksum="C0433B8932C22949" modified="2013-10-16" version="1">MKVIWILFVALLLLAISISTSFAKKKESSGGEEESGEEYGMEKHGKKGHKGDKGHKVSHHYDKGEKKKYDEGDKKHHHEEKGGTKKKEWDEEEKHGKHSEHGEKKKGGRNTTRKVTRRKKKFYDDEDKGGHHKKHKKGHHYSDHHSKGMKKGEYEHGEHKKEKKHKKGHYDKGHHDEEHKSYKKEHGHDEKHGHKEGHHKKGHKKGGHKHGHKKGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H053</accession>
    <name>T1H053_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WDR59/RTC1-like RING zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H053"/>
    <dbReference type="STRING" id="36166.T1H053"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009521-RA">
        <property type="protein sequence ID" value="MESCA009521-PA"/>
        <property type="gene ID" value="MESCA009521"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050289_0_0_1"/>
    <dbReference type="OMA" id="VICKIAV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR59/RTC1-like RING zinc finger" evidence="3">
        <location>
            <begin position="270"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009521-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="330" mass="37212" checksum="0EC1264E377A5D0B" modified="2013-10-16" version="1">MGTQPVLYAHRESNIQDRLNTQITYLKLYTKSSRSKSIHKPITSPVVQIYDFKRLLFLNKEIAMNFEIHKSDLVGTCRRSQKIVEANGRPDLVPIWMLAELIASNCTPLEENCGELLFSNDPFKKNILESLILHFAINGDIQTAVMLCAVFHKCCPENEPATKNILSPMGSPYHTVLPIDKFNTHLSEQSLAMQLKQNRSNSWSDSLDVQDSLKTLHQEKEIFTCSLIKKSITIYSQTLRLFKKGVEFAAECKNCGKPNKSPSCNSCKNMIMYCIICRLPVRGSANVCLICGHGGHTKHLESWFLNHDICPTGCGCQCLEENVALLEKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0L9</accession>
    <name>T1H0L9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0L9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009700-RA">
        <property type="protein sequence ID" value="MESCA009700-PA"/>
        <property type="gene ID" value="MESCA009700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1717804_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009700-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17310" checksum="FD8B37E7489DEBC5" modified="2013-10-16" version="1">FYLKLLPDLSKFPNDITGKLPGGGGGGQGSEAFSVRTYQDTVKKILDEAINSMKSFAEIYTKGVDKLITDVETTTIKDIKKSIQASEGLEKLTKTALAEIEKIEKKLDEIETLMSKKDFYIRQEYNIARYKLDTSVSAFHWDYYGRANYVWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H156</accession>
    <name>T1H156_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucosamine-6-phosphate deaminase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H156"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009909-RA">
        <property type="protein sequence ID" value="MESCA009909-PA"/>
        <property type="gene ID" value="MESCA009909"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834090_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009909-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="6751" checksum="95968A68D9C4CBC3" modified="2013-10-16" version="1">MGIGTILAARKTVLVATDSAKVESLVCAIEEPLSACVPASALQLHPDCLIIADQGAASALTQYNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H170</accession>
    <name>T1H170_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H170"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009924-RA">
        <property type="protein sequence ID" value="MESCA009924-PA"/>
        <property type="gene ID" value="MESCA009924"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349046_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009924-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11210" checksum="214AA71D8BDF18AE" modified="2013-10-16" version="1">MVTLSINETKVENLGIIMNSSLQWEDHIAKMSFEQYAKLFLESIMGEMNSRCLGELERAFNSMTVNYLRNLHVNYACKLQTVPTPTEYTCFTENDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1J2</accession>
    <name>T1H1J2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1J2"/>
    <dbReference type="STRING" id="36166.T1H1J2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010054-RA">
        <property type="protein sequence ID" value="MESCA010054-PA"/>
        <property type="gene ID" value="MESCA010054"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="OMA" id="TMHSADI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010054-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="238" mass="27727" checksum="3771CEF250BE269F" modified="2013-10-16" version="1">MELCIWIISFARKIIDIRDILRVDSIEQMKDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANICDFGLARVADPDHDHAGFLTEYVATRWYRAPEIMLNSKGYTQSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILSVLGSPSQTDLECIINDKARNYLESLPFKPKVPWERLFPNACPKALDLLDKMLTFNPNNRITVEQALAHPYLEQYYDPGDEVLREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1V8</accession>
    <name>T1H1V8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">40S ribosomal protein S23</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the 40S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1V8"/>
    <dbReference type="STRING" id="36166.T1H1V8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010178-RA">
        <property type="protein sequence ID" value="MESCA010178-PA"/>
        <property type="gene ID" value="MESCA010178"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_1"/>
    <dbReference type="OMA" id="KFRWSQR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22119" checksum="A75976CA08B90E42" modified="2013-10-16" version="1">MVSLEESKISSFHVIRLFFFIRKCACLIFSVSENLLILKFPRTNLLNSNSKSQTGKPRGLRTARKHVNHRRDQRWADKDYKKAHLGTRWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPRDGSLNYIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKEKKERPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2B9</accession>
    <name>T1H2B9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2B9"/>
    <dbReference type="STRING" id="36166.T1H2B9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010359-RA">
        <property type="protein sequence ID" value="MESCA010359-PA"/>
        <property type="gene ID" value="MESCA010359"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104868_1_0_1"/>
    <dbReference type="OMA" id="HERRQSY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF1">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010359-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13539" checksum="A69D744E64FEDBA8" modified="2013-10-16" version="1">MSLSYKKCYFHQPGTAPLIYRNIGQQLDLSAKEFSNVEAVVACSEGKRLTYTELNAQVSKLGAGFLKLGLKPGDALGIWAPNYLHWYISMLAAARIGLVTVGINPNFQASELKYALNKVKCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2F5</accession>
    <name>T1H2F5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2F5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010400-RA">
        <property type="protein sequence ID" value="MESCA010400-PA"/>
        <property type="gene ID" value="MESCA010400"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164973_0_0_1"/>
    <dbReference type="OMA" id="HLHGSFH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577832" evidence="1">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010400-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10027" checksum="EFE2CE3C7F152EBE" modified="2013-10-16" version="1">MLILLICISILAKCGSYDPNDKKIASVLPPEGMFEAFYPREMDGLPNGSSRPAHSHGSFFKYRNPALVDIKNAAAWGFRFDGKRRFNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H343</accession>
    <name>T1H343_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02135213">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02135214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H343"/>
    <dbReference type="STRING" id="36166.T1H343"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010656-RA">
        <property type="protein sequence ID" value="MESCA010656-PA"/>
        <property type="gene ID" value="MESCA010656"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344633_0_0_1"/>
    <dbReference type="OMA" id="ENATWIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF127">
        <property type="entry name" value="FI08437P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="22763" checksum="37F52E8C39B3F660" modified="2013-10-16" version="1">MLMIGRFIVGLASGLTFTALPMYLTEIAPLELRGTFGVFCAVGVTAGVVVGQVFSLKVIFGTESLWHYALCFYVVLIIICYSPVFIFPESPKYLYIIKRNREQALRELQKLRGKNAQSQIEKEMEDMELESNQTSEIKTIRSILCDPSLLLPIVIVCAFQGGQQLSGINAIFYYSVTIFKEAGLSQENAEWANLGAGWLSEKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3H1</accession>
    <name>T1H3H1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3H1"/>
    <dbReference type="STRING" id="36166.T1H3H1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010798-RA">
        <property type="protein sequence ID" value="MESCA010798-PA"/>
        <property type="gene ID" value="MESCA010798"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1950994_0_0_1"/>
    <dbReference type="OMA" id="ITRNKIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010798-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15369" checksum="F51210A28A72F5D7" modified="2013-10-16" version="1">TLEGEGVSGLSKLDLYNFFAPWYAEHLGTIRSASGNATVEDWARKKLTYEYYVIKYITILKLQGNGRLLNSLDHLLGNEAILQLDLKSLKVIFQNDEQKKWFTEVLDNQIKLWEVNMNQKNDPNDTIRGNLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3H3</accession>
    <name>T1H3H3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374533">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3H3"/>
    <dbReference type="STRING" id="36166.T1H3H3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010801-RA">
        <property type="protein sequence ID" value="MESCA010801-PA"/>
        <property type="gene ID" value="MESCA010801"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095549_6_1_1"/>
    <dbReference type="OMA" id="YCGNYIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010801-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9560" checksum="C78CFE1327C27453" modified="2013-10-16" version="1">YPKRKKSVKQNVVQKLNTTICPSCNAEIETSTRTEPGMIAYLSGLVIALLGCWFGCCLIPCCIDECMDVHHTCPNCRAYLGRHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3I5</accession>
    <name>T1H3I5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02019680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3I5"/>
    <dbReference type="STRING" id="36166.T1H3I5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010813-RA">
        <property type="protein sequence ID" value="MESCA010813-PA"/>
        <property type="gene ID" value="MESCA010813"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_9_1"/>
    <dbReference type="OMA" id="LPMTICG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000278">
        <property type="entry name" value="Protein CBG07490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="2">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010813-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15842" checksum="9137B50624457E35" modified="2013-10-16" version="1">MIDIEAPVTVCGDIHGQFFDLMKLFEVGGPPSSTNYLFLGDYVDRGYFSIECVLYLWALKICYPTTFFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEIHDLDDIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3T7</accession>
    <name>T1H3T7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049014">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3T7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010925-RA">
        <property type="protein sequence ID" value="MESCA010925-PA"/>
        <property type="gene ID" value="MESCA010925"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530034_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010925-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9407" checksum="033E03528001352D" modified="2013-10-16" version="1">MPGKSTTDKFFTLKRMILQIGDGDDLFRAMNQFCTPSKIQEDPHKATERSGQYGRIDESGPTRKACFSREVGICGSFISSKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3V6</accession>
    <name>T1H3V6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02029449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029450">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3V6"/>
    <dbReference type="STRING" id="36166.T1H3V6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010950-RA">
        <property type="protein sequence ID" value="MESCA010950-PA"/>
        <property type="gene ID" value="MESCA010950"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_730712_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010950-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="379" mass="44481" checksum="E95D808286995666" modified="2013-10-16" version="1">DYTIGRQPNCDNFHNLIHIYDDVLNFGPLDNFSAFKFENFIQSLIKDIRKPEKIIQELYHRYMERDTIENAKKLTDFNEENFNIEENADAFENAATDALETVERLKTRFSKVGYRLKCTAFKIKKFVNTFKLYEHNLPSDQRTLLSTPRKCPINICEFGEYKYFGVESSLEKILEKNRIEFLPTELSLTLCIDDVPINKEVSITATFNIEMSKFIADTVAKSKILKIKGHTGYLRCTKCWVHGELKNNRLYFKDTRAKKHYTIGRQPNCDNFHNLIHIYDVLNFGPLDNFSAFKFENFMHFLIKDIRKPDKIIQQLYHRYMERDTIENAKSNEKTFKNITAPGPTVDNMVGIHYSKIKFGNFIINTKNNGDNCFQIGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5B2</accession>
    <name>T1H5B2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5B2"/>
    <dbReference type="STRING" id="36166.T1H5B2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011489-RA">
        <property type="protein sequence ID" value="MESCA011489-PA"/>
        <property type="gene ID" value="MESCA011489"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_497240_0_0_1"/>
    <dbReference type="OMA" id="TLEACPM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="13"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="257"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011489-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="548" mass="59736" checksum="8E3B76AEAF78E5CD" modified="2013-10-16" version="1">MLRKRNNIEDSSDSSSSDSSSASSSGSERNSDSSSDSESGSASSDSGSSSNSSSNDDNNGKNEVPSTEKKESPKKPTSVRNTVNNNSNRNTNKSLERKTTASSTKAIKKTQPTTASKQTNSSDDDSTSSSESTPAPSPKKIPKVMSSSVAKRAAANKQQQQQPQTSSTQHSHPPGGGKAPPSVVKQHQQNFVKSQQNRAKNPITKPGSILKTKKKAQNKKTANVESSDEESSEDSDRKRKKVTTQKGNWKLIEKSKTATYSSSSSSSTTSSSESSDEGSNSQSSKSSEKTKKPTTKPLKKSSSGVNLSESEKDDKETQLVTPTKNPIRKLTRSSSTRKSKHVNPKKTSESESELDIDKRSSTKSPAKRPSNSKVKRDLRDDSDRSPPIPPPPTPPPDEQKCPVEGCDSSGHLSGNLDKHYLPEACPIYHNMSTSECKERYAERKLRDKEIDKILGSYKNCTDKQAMIAEQKAFLAKIHEKRNHFEEQKNLKGGVDPAKKEIECDIAREPNLVGLTSEYDLNLFREAQAIASERIEEELKDVVVGKGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5J0</accession>
    <name>T1H5J0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5J0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011568-RA">
        <property type="protein sequence ID" value="MESCA011568-PA"/>
        <property type="gene ID" value="MESCA011568"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253088_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011568-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12349" checksum="F9678FB91601C9A8" modified="2013-10-16" version="1">MLEFMGDRNQARKFYENNRSGDLITNKEEVLMQCEDFFKELQEIPSRKSVPEMRSLDMVICRAIPLLHDDLYHHCLHMSLKLNVLENTIINKITDITSKRLIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5T1</accession>
    <name>T1H5T1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5T1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012064-RA">
        <property type="protein sequence ID" value="MESCA012064-PA"/>
        <property type="gene ID" value="MESCA012064"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663197_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF211">
        <property type="entry name" value="LD43457P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="415" mass="47231" checksum="F1FF36F8898FCEC9" modified="2013-10-16" version="1">NDVEAAIAQSKALVMSSSRQQLQHQQLQLLQTQQSSSSIQALQQTVMLQATTTQQCKRQPQTISRSSSAAKSMVNSVFGDIHELLNIADVADLLHPQHAIITGGKSKDGCPLIIFPDNNNFQSLDEMDYRKLIVYLYSVPSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPSAVQSQSPLHRHQLHDQINKQCMKNVEHFDDDCWVYRRLLVQLEETERRFDEFWRTHKNRLNHCLHLRRFEQDFRELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Y2</accession>
    <name>T1H5Y2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5Y2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012115-RA">
        <property type="protein sequence ID" value="MESCA012115-PA"/>
        <property type="gene ID" value="MESCA012115"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3210335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006588">
        <property type="entry name" value="Peptide_N_glycanase_PAW_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51398">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAW" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012115-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="45" mass="5009" checksum="30C81E697E0B1BEA" modified="2013-10-16" version="1">GSPLKNRKITNNNRQTKRGASAGSIMWRFYTDDSLGIKVGLFPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6E8</accession>
    <name>T1H6E8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cleavage and polyadenylation specificity factor subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPSF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6E8"/>
    <dbReference type="STRING" id="36166.T1H6E8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012281-RA">
        <property type="protein sequence ID" value="MESCA012281-PA"/>
        <property type="gene ID" value="MESCA012281"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2298730_0_0_1"/>
    <dbReference type="OMA" id="THNEHLC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.910:FF:000005">
        <property type="entry name" value="Cleavage and polyadenylation specific factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012281-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11633" checksum="8CA8504832426210" modified="2013-10-16" version="1">GGLGYCLPLPEKTYRRLFMLQNVLITHNEHLCGLNPKDFRTIKSTRKTSLNPSRSIVDGELIWSYLMLTQSEKQEIAKKIGTKMEEIYADLLDIDRVSTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6P0</accession>
    <name>T1H6P0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6P0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012373-RA">
        <property type="protein sequence ID" value="MESCA012373-PA"/>
        <property type="gene ID" value="MESCA012373"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009879_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012373-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="723" mass="82162" checksum="04F7BC1A973C9309" modified="2013-10-16" version="1">PPPKIIRLRTLNAKSNCAALAREVVDKCDLIHKSQLSEVEQIIFYLKNRKDNTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGSLCMDIIEAELRRYESMRNKVDTPKNCSTSSAKAEKXXXXXXXXXXXXXXXXXXXXXMEPPRRRIPELKQRPKSGNWSSFHSSMSSSLSKSQILNNSYHEALDVTSTTESNKSNSSEALDSKKEEYDRLNKQLKIFAKKQEQLLRLAFYLLLNVAENVKLEEKMRRKNIVHMVVKCLDRQNIELLTLAVTFLKKLSIVTDNKNMNELNITSKLPRLVQCGHADLVHVTLKLVFNLSFDGQLRGKMIKAGYIPMLVMFLGDEKHHSIVVKILYHMSLDDKVKSLFTPTECVQMATDAIILNLNTKVDLDLISLCINLALNRKNAQIMAEGSRLNDLFERAFKLKDTLLMKLLRNISLHSSVHSKFIDHVDDLVKNMLNFDDEGFMIECLGILGNLRLPDLDYSQMVQNNDLIPWIQANLIPGNKMDDLILDTVVFVGTCSGEELCATLFCRANVVLSLIDLLKAKQEDDEIVLQIVYAFQQILKHESTRNYMIKETESPAYLIDLMHDKNSEIRKVCDFCMDIIAMTDSDWATRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6P7</accession>
    <name>T1H6P7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6P7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012380-RA">
        <property type="protein sequence ID" value="MESCA012380-PA"/>
        <property type="gene ID" value="MESCA012380"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270478_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF7">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012380-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11735" checksum="9E0EE3B55FFECF95" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVIRPENSGNWTLQIKFPQLRDSGIYECQINTEPKMSLSYTFNVIGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7K3</accession>
    <name>T1H7K3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7K3"/>
    <dbReference type="STRING" id="36166.T1H7K3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012740-RA">
        <property type="protein sequence ID" value="MESCA012740-PA"/>
        <property type="gene ID" value="MESCA012740"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152291_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012740-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="14290" checksum="2F0243A7C4CF305F" modified="2013-10-16" version="1">HRCLGKYMYTGDTQYSIGSVGYTDVDCNFIDFTYVCCTSERLNQTVRQAINQFSENLRCNESSGSTGQISLEFFQKKKPRWSFTTNTPWEVWTIRLELKNSQNEDERQRCREAVSEMLTDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7L5</accession>
    <name>T1H7L5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the complex I NDUFA12 subunit family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7L5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012752-RA">
        <property type="protein sequence ID" value="MESCA012752-PA"/>
        <property type="gene ID" value="MESCA012752"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110455_1_0_1"/>
    <dbReference type="OMA" id="WHGWIHH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B17.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012752-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="13166" checksum="BB37E9AC635DCD76" modified="2013-10-16" version="1">FDELKLGTLIGTDKYGNKYYENPYYFYGRNRWVEFADHQNMDYDGSQIPAEWFGWMHYKTDLPPIRDGSRVQYKWMANHSENLSGTKEQYMPYSTTKPKYEAWDPKKKX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7L6</accession>
    <name>T1H7L6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7L6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012753-RA">
        <property type="protein sequence ID" value="MESCA012753-PA"/>
        <property type="gene ID" value="MESCA012753"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106880_0_0_1"/>
    <dbReference type="OMA" id="YDIEWNF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029686">
        <property type="entry name" value="Malpha/m4/m2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12254:SF0">
        <property type="entry name" value="BARBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12254">
        <property type="entry name" value="ENHANCER OF SPLIT MALPHA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15952">
        <property type="entry name" value="ESM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012753-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="15239" checksum="A2DE757261629846" modified="2013-10-16" version="1">NNTKRSKVSASRRIKNYLKPVLGKMFKSKEPKRNSYVEVTKAMTGWMNMNMANEDIENRIIEEVQSLPDDAAIFIINEDGDQTLQYINKDKFTVPVHFAQTESGTLFWTSPMKTINAFKIEWTFLDRWAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA21</accession>
    <name>T1GA21_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA21"/>
    <dbReference type="STRING" id="36166.T1GA21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000064-RA">
        <property type="protein sequence ID" value="MESCA000064-PA"/>
        <property type="gene ID" value="MESCA000064"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000036">
        <property type="entry name" value="Trypsin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF95">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588132" description="Peptidase S1 domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="32"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="267" mass="28883" checksum="178E9F2B605615CF" modified="2013-10-16" version="1">MKVLLTSFFALLTFSSASIQISKSGPVYSTNVIDGVISIRGEAPYIVSLQNIGGHYCAGSIIYENWVLTSAHCLTYPNIKVVAGLFNRFDLTGTQIRSISNFSHIIPHPKAFILNNKTSVDNSKKVLLLKPINSLVLSNPVNSILLPDANFNFSGDGTVYGWGTMRSGNMSDTLQKLDTGVLEYPQCKKELPVSAPLAPINICSFGKGSNKYEGACNGDSGGPLVQKGLGGTVLIGLVSWGYIPCETTPYPSVYTSTKQYRNWIDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAP7</accession>
    <name>T1GAP7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000306-RA">
        <property type="protein sequence ID" value="MESCA000306-PA"/>
        <property type="gene ID" value="MESCA000306"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_877966_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000306-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="317" mass="34647" checksum="0242BCDDFFBA2370" modified="2013-10-16" version="1">MCLDAIPENPNISKQMLRKAKTMTGGLSIPVINVSASQSSVPININDSKISNTKSRTKPRGNKTLKDLEEPMEISSFEKSSTTSQNKTVVKATSMCLEAIPENNISRKRLRRAKTMTDTIYNSVDMSEVETVPVKNVSASQSSISMNIDESTITDKSRNRSLEKSSSVPQNKTVAKATSMCLEAIPENSISRKRLRRAKTITDTICNAVDMSEVETVPVNDASISQSSISMNIDESTIKDKLRNRSLEKSSGVSQNKTVAKASSMCLEAIPENSISRKRLRRAKTITDTICNAVDMSEVETVPVNDASISQSSISMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAR1</accession>
    <name>T1GAR1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dehydrogenase/reductase SDR family member 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GAR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000321-RA">
        <property type="protein sequence ID" value="MESCA000321-PA"/>
        <property type="gene ID" value="MESCA000321"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003151_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000321-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="30919" checksum="07B26B1481058D4E" modified="2013-10-16" version="1">EDRLNELKNSLPEELRSKVHPIRCDVTNEADVVRAFRLVEFSLLKRMDRWANKAAIVTGASSGIGSACVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGMVVAALARREDRLNELKNSLPEELRSKVHPIRXCDVTNEADVXXXXXXXXXXXXXXXXXXXXXXWVDEHLGGVHVLINNAGITRSTELTKKDNTRDIRDIVDTNVMGVVYCVREAYHQMVDHKVDGHVVIINSIAGHSVPYVPGSSFNIYAATKHAVTAMVETYRQEFSNSGTNIKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB16</accession>
    <name>T1GB16_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02110855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB16"/>
    <dbReference type="STRING" id="36166.T1GB16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000436-RA">
        <property type="protein sequence ID" value="MESCA000436-PA"/>
        <property type="gene ID" value="MESCA000436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2055581_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043458">
        <property type="entry name" value="GPR158/179"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 158-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546:SF29">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22673">
        <property type="entry name" value="MCP-like_PDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="14011" checksum="B0B9BB01C20E496D" modified="2013-10-16" version="1">VVNLRVWNRGTFHQYTNSTSERIEENTLAVKYEDGKWSKPYYDCGGGNIWMLTYTVPFFGYENGTYFFKYIIYQLKSSLYFDAGTVARCNPRTQVIGIPHYFDVVTTHVDIWAKCVMISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBE7</accession>
    <name>T1GBE7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBE7"/>
    <dbReference type="STRING" id="36166.T1GBE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000579-RA">
        <property type="protein sequence ID" value="MESCA000579-PA"/>
        <property type="gene ID" value="MESCA000579"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673929_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019321">
        <property type="entry name" value="Nucleoporin_Nup88"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10168">
        <property type="entry name" value="Nup88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000579-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8707" checksum="B87008DE4FC8150D" modified="2013-10-16" version="1">METVELELGLPDTDIKSSFTSPINLKRDYMIEHRYFVYHNTGLHAVSINFIDELQNYVEGNERKSLFPIISSCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBK7</accession>
    <name>T1GBK7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBK7"/>
    <dbReference type="STRING" id="36166.T1GBK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000641-RA">
        <property type="protein sequence ID" value="MESCA000641-PA"/>
        <property type="gene ID" value="MESCA000641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172071_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="13310" checksum="00C4C3EE7B5F6A2A" modified="2013-10-16" version="1">MIVLLGQQRRFEALDFCYHILRIQRVDGKDEDVKGVKLKLMTDRIRRFQVLNSQIFAILNKYLKSSDGEESNVEHVRCFPPPQHPTMVSSHYHDPNKLRQQQQQQQIQLTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBZ6</accession>
    <name>T1GBZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBZ6"/>
    <dbReference type="STRING" id="36166.T1GBZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000789-RA">
        <property type="protein sequence ID" value="MESCA000789-PA"/>
        <property type="gene ID" value="MESCA000789"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973023_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14394" checksum="92ED1A226038C6FE" modified="2013-10-16" version="1">MKTLTPSNMSSINDKSEVLPFVSNHMQLIKKGFLVGSTLNIIMKFMSKEVKSRLTIHDGKRFDKLYDVLGYANVPKEYGGPTDYDLLTFGGEVQSSAQQLFEFIKNKWAVILKVQSYRKILNAGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC60</accession>
    <name>T1GC60_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC60"/>
    <dbReference type="STRING" id="36166.T1GC60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000858-RA">
        <property type="protein sequence ID" value="MESCA000858-PA"/>
        <property type="gene ID" value="MESCA000858"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1572427_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026728">
        <property type="entry name" value="BLTP3A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774">
        <property type="entry name" value="CHOREIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774:SF11">
        <property type="entry name" value="CHOREIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24917">
        <property type="entry name" value="BLTP3A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000858-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19446" checksum="7F52CEC5FF3301E1" modified="2013-10-16" version="1">MPYFSSTFFRSCVKLENVRIKLTEDRPPVNITSPGPVPVNLAIGSMKIKRDENGILYIQPSEGKDDENRRTQDNSCHHDRDREILSLQLVMQQIKMDNDNLKKQLVSSKENSESFRQKTKQDQDVLKACLKAAQDDISILLEEKKALLDTIRSLQTQLTTTSNQKNVGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC98</accession>
    <name>T1GC98_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000906-RA">
        <property type="protein sequence ID" value="MESCA000906-PA"/>
        <property type="gene ID" value="MESCA000906"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742946_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000906-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8141" checksum="0CFF691DCEDB779B" modified="2013-10-16" version="1">MWLRAQVSLTPKFNFKHHVLCDQHLASSITDIRSIRDSSHSCPYNSAYIFSTNSLLYKLPSSARFNSVHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCB8</accession>
    <name>T1GCB8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Thyroglobulin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003128">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCB8"/>
    <dbReference type="STRING" id="36166.T1GCB8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000928-RA">
        <property type="protein sequence ID" value="MESCA000928-PA"/>
        <property type="gene ID" value="MESCA000928"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023483_0_0_1"/>
    <dbReference type="OMA" id="NWERKEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF30">
        <property type="entry name" value="FI05255P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thyroglobulin type-1" evidence="8">
        <location>
            <begin position="69"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="9">
        <location>
            <begin position="220"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="8">
        <location>
            <begin position="273"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000928-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="48535" checksum="5E70197C0B14A88D" modified="2013-10-16" version="1">TQLSIDSKDKPSSNNLPFGSEYDESEDYETEEEYDGLGELNRSNIPNLNGILTSLQQNSVLFKEAETETSGCIADRKTASEEQHRANPMLYVPECTSDGRYEKIQCYPRTGYCWCVDEDTGKVLTGTSVKDKRPECKHVARPFKGCPEPHKTKFLRDLKVFFTTSIPLENNASNGTTFKSEDEKIGILSFVHIDKNKNRNWERKEWKEFRELVGKSKLYGNVISECATKKLECDQVRERPVCGTDDKTYPSRCHLLRAQCSGHEVSVKHRGPCKECQQARQYALKNNYQSRKEFVPKCRSDGTYSAVQCVEEHGCWCSDSSGKPIANTTTKNGKPNCKKFLRGIRRSSPRHMNTMTVQRDCIKADQTAFIANVIQRFTSEYKGYNDAHHTTKPKQCARAFAKLCDTNNDERLSLIEWKLCFSPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCF1</accession>
    <name>T1GCF1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02077872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCF1"/>
    <dbReference type="STRING" id="36166.T1GCF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000962-RA">
        <property type="protein sequence ID" value="MESCA000962-PA"/>
        <property type="gene ID" value="MESCA000962"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052282_0_0_1"/>
    <dbReference type="OMA" id="ALTCETF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000962-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="12672" checksum="05C6EB8901A67794" modified="2013-10-16" version="1">MGSIAQTIFMLGVFTGAVTLGGLADKIGRKTVFCWSGLIQLFLGVGVAFIPEYFSFLVVRFLLGIVGSAGAYICGFVLTMELVGSSKRTVCGISFQAVFAGGIMLVAGWGALIKIVLYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCQ2</accession>
    <name>T1GCQ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCQ2"/>
    <dbReference type="STRING" id="36166.T1GCQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001072-RA">
        <property type="protein sequence ID" value="MESCA001072-PA"/>
        <property type="gene ID" value="MESCA001072"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1385622_0_0_1"/>
    <dbReference type="OMA" id="MGHRINV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577273" description="Farnesoic acid O-methyl transferase domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="3">
        <location>
            <begin position="36"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001072-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22933" checksum="C787BD9AF32E477D" modified="2013-10-16" version="1">MFFALLITSVLSSTQAYNTNLSYSVLSECKEYQIDGQYKYRDAFTILDMKNNKPFENEIFRLKLYVFTKQDAHILISNEPRVHKGESAYEIVFGGYDNTESCIKVHRLGPQVVRKKGEVTSPLYPTEIIIALREDGELSVILPDFERPYMVYKDKSARYWTYLLFPLGIIMLHVGFMIVHWGMMGHRINVYVLLPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCW3</accession>
    <name>T1GCW3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydantoinase/oxoprolinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390008">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCW3"/>
    <dbReference type="STRING" id="36166.T1GCW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001136-RA">
        <property type="protein sequence ID" value="MESCA001136-PA"/>
        <property type="gene ID" value="MESCA001136"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387331_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="1">
        <location>
            <begin position="57"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001136-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22230" checksum="7FA4CEFE2B055851" modified="2013-10-16" version="1">TVFCTPLSFYQLEFVVEFLFLSRLSRWKPIKSEFHGIRTQSTSVVQQVKMSNGKYNFAIDRGGTFTDVLCVRPDGSARTLKLLSEDPANYSDAPREGIKRILQQETGNALTADGLVDTSKIGWVRMGTTVATNALLERKGDPVVLVVNKGFRDLLYIGNQARPKIFDLNIKKPSNLYQKVVEVDCRIVPEQKERCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD84</accession>
    <name>T1GD84_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001265-RA">
        <property type="protein sequence ID" value="MESCA001265-PA"/>
        <property type="gene ID" value="MESCA001265"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1715350_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001265-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17948" checksum="E0E5B4DCF18CEDB7" modified="2013-10-16" version="1">MNLRYLRNKSHLDHNCGSVSENRWEDSERLLPKCIRSLAMATYLDNFFTYIQIRHLSRRAKIAIVVRRRKKIKTSSQHPGRGNESFLLPRGVHFNRRTVNRKYLRRTAKICAAVEQPVPELLYLGYMTAENLRLSVCLTDIVECQLICTKFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDK6</accession>
    <name>T1GDK6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDK6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001395-RA">
        <property type="protein sequence ID" value="MESCA001395-PA"/>
        <property type="gene ID" value="MESCA001395"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770194_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001395-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16677" checksum="CDEB4ECB9C1CC258" modified="2013-10-16" version="1">MFKIPFYSTVALTYDLATAEKRPTVPTTPRQLKYRRTQLTRAPTFSPMPGHSKQDNKTAKEPVYPVVNRLQQGRTGAADYRNKETTPKRQPCEGFQGIPIDKGNRSTNSQLIKGLQPRPSPRRVNKTRKEPVYPVLRPTAGRDRSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDZ5</accession>
    <name>T1GDZ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDZ5"/>
    <dbReference type="STRING" id="36166.T1GDZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001550-RA">
        <property type="protein sequence ID" value="MESCA001550-PA"/>
        <property type="gene ID" value="MESCA001550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1373640_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588212" description="Partial AB-hydrolase lipase domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="2">
        <location>
            <begin position="50"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="22541" checksum="F1F9E566190EC419" modified="2013-10-16" version="1">MKRITLLVCFSVPFALAGDSLFDTSSSTAVSFYFELYPINPSAGKFPSNRSHGFPVEEHSVQTEDGYILSIHRIPKANGSVVFLQHGLLSSSGDWLIIGPQKALAFVLSNQGYDVWMGNARGNTYSRKHKTLSPESSNFWNFDWHEIALYDIPAMINYVLNKTKQRKLNMWVTLKERRCILFSTPKNQSLAKNSDLLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF23</accession>
    <name>T1GF23_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001952-RA">
        <property type="protein sequence ID" value="MESCA001952-PA"/>
        <property type="gene ID" value="MESCA001952"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177365_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001952-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12596" checksum="B8216C053D078428" modified="2013-10-16" version="1">MVKVLLAGAFRSIIKDKVSKTVGSDNVFPAMLQNTLRNQASFKEFYKIKDALAAYGKLVPPEALPANVSENQQKKHREEKNLYRRQTIMLSQCWQVQFLTTQFPSMTESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF25</accession>
    <name>T1GF25_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA001954"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SDR_fam"/>
    </dbReference>
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        <property type="entry name" value="FI23927P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13484" checksum="AA4EF86C9AB0CCC4" modified="2013-10-16" version="1">MIEKDHGHIVTIASLAGHVGISKLVDYCSSKFAAVGFDEALRLELDVLGHHGVFTTCICPFFIQSTGMFDDVNARWVPTLDPNVVADKVIRAIKEKEKCAILPGYLKAVLPLKLSSLGDVLEGF</sequence>
</entry>
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    <accession>T1GFW1</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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            <taxon>Brachycera</taxon>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
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        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1GFW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002263-RA">
        <property type="protein sequence ID" value="MESCA002263-PA"/>
        <property type="gene ID" value="MESCA002263"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002263-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="53" mass="5779" checksum="7ED924E27BD9841C" modified="2013-10-16" version="1">MSDGLRGDHSFSHLGSSDDGNISDFYDPRQSPPKAIVDSSPLQPAMDKNKECK</sequence>
</entry>
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    <accession>T1GGL3</accession>
    <name>T1GGL3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <authorList>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="gene ID" value="MESCA002535"/>
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    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002535-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="8074" checksum="2A7B81557BA84759" modified="2013-10-16" version="1">MTYIFSTKTFLEAIRGIQVFTNKRSQFYYSRAPSGETYNSILLLALLVLQGFVYGLLYFYFSKVFPGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGX6</accession>
    <name>T1GGX6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02064511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064515">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGX6"/>
    <dbReference type="STRING" id="36166.T1GGX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002657-RA">
        <property type="protein sequence ID" value="MESCA002657-PA"/>
        <property type="gene ID" value="MESCA002657"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_565368_0_0_1"/>
    <dbReference type="OMA" id="NNTICGK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002657-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="483" mass="54052" checksum="AAEC4BE7CFEBAF7A" modified="2013-10-16" version="1">MTYDAIENTSFQMVNRTEVIASDNATFVDSKNGDDTFTKPFLNETFEKPSTGTPESSFRIEQSTPHHTKFAPGKRFTPNFRGMIEDEINISPITSLKNPVARDEGFADGTYEDNILKASCEGSNEEDFYDMLDKFGKYTPSAEKVKIQKTVDSIKKKYVQSGNVEEKSPLATSFVKESVESCSNSGSMTSSGNVCNKLTTSATSLTSSTDRLLNRRGRLYDDIQMNISQKSLSLKESITTSNGGEKDENTAATNSESLEGGLDQIEEQQTPSLADQQPLENEKRKTDIVNKKSGKIRDHDRFKTIKICKRSEESNDQQLNVPCIDDNELDQNPNQEQNYEFQEFEHEQDFTEEPQRFSRKDSHFNSPSNFLTYKKPKEKPVEPIVPITEYNKQSNAARSLSRPRYISGLQKLSLSNKDSMAKAASADDLEKSSSTSSTTDLTEEIKAPTVNRFGLKKFSVPGAQSRPGDIKSPMGNKAKLYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH36</accession>
    <name>T1GH36_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH36"/>
    <dbReference type="STRING" id="36166.T1GH36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002720-RA">
        <property type="protein sequence ID" value="MESCA002720-PA"/>
        <property type="gene ID" value="MESCA002720"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1322248_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002720-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="23285" checksum="D5CBD1391780A723" modified="2013-10-16" version="1">MTASIGSNGTILSLNDSNSSLGGGTVITYLKSNPKYKNIFAICGPIFREALVSNGFTIDDKPDETERFTLQTFTQNIIPTAPVDAVIIDIDLDMSLAKFCRGLLHAKNYPDCDIIVGATDNKIPLSKDVVIPGTQVLMDYIDITLERNLLSLENLENNWEIILWNCTTSHVLKGVYLLATICQQILVLQTHWDFKPYLFCRERIPMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHA0</accession>
    <name>T1GHA0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glutamate synthase (NADPH)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHA0"/>
    <dbReference type="STRING" id="36166.T1GHA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002790-RA">
        <property type="protein sequence ID" value="MESCA002790-PA"/>
        <property type="gene ID" value="MESCA002790"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_3_0_1"/>
    <dbReference type="OMA" id="NRRIEQM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000113">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000124">
        <property type="entry name" value="Glutamate synthase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate synthase alpha subunit C-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="346"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002790-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="74785" checksum="9A51FA52D00BE136" modified="2013-10-16" version="1">MNYEFKHRVNVILHGDANDYVGKGLCGGKICILPPETSTFESHLNTIVGNVCLYGATSGRAFIRGIAAERFCVRNSGVTAVVEGVGDHGCEYMTAGLVVILGLTGRNFAAGMSGGIAYVFDIDGSFKPKVNAESVELLPLESEKDIKTVKDLLCEFIKETKSIIAKEIYDNWCDYQSKFIKVFPYEYQKALKEMVVNVEAPIIENSIKDIEESIRDMDLEKKQADKILDKNRGFIKYKRESDIYRDAKERQNDWNEVYNFKHVRKNLKMQAARCMECGVPFCQSNVHGCPLEFTGRVCPAPCEGSCVLGISEPAVTIKNIECAIIDHAFEQGWIKPEIPAHRSGKHVAIIGSGPSGLAAAQQLNKAGHKVLVFERNDRIGGLLQYGIPTMKLSKDVVKRRVDLMEAEGIEFKTNVHVGKDIKAEMLLNDFDAVLITTGSTWPRDLPLANRNLNGIHFAMEFLEAQQKSQLIGNNTPISAKDKDVIIIGGGDTGCDCIATSLRQGAKTITSFEILPEPPAKRAYDNPWPQWPRVFRVDYGHEEVKVKHGHDPRKYVTTTKEFVGDSSGNIVGVNTILVDWTKGPKGEWIMKEISGPEKTVPNELNLELDNRGNIKNCIGNYGTSNKKVFAAGDCRRGQSLVVWAITEGRQAARQIDDYLLNKESILPGPGGIRGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHM4</accession>
    <name>T1GHM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHM4"/>
    <dbReference type="STRING" id="36166.T1GHM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002931-RA">
        <property type="protein sequence ID" value="MESCA002931-PA"/>
        <property type="gene ID" value="MESCA002931"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932812_0_0_1"/>
    <dbReference type="OMA" id="VPEWIES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF1">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002931-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14900" checksum="BBF199DB5638B7BD" modified="2013-10-16" version="1">MLGLDVQGAKLITTFKGHTGAIKCLKWVEGPQLLFSGSFDNSVIVWDVGGRRGTVYELQGHNNKVSALRYGNHTQQLVSAGEDSVVVFWEMNAMRKEVPEWIESNNCQLCSRPFFWNFRAMMDQRQLGEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHP2</accession>
    <name>T1GHP2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002950-RA">
        <property type="protein sequence ID" value="MESCA002950-PA"/>
        <property type="gene ID" value="MESCA002950"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796908_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002950-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7572" checksum="06768E36436545F8" modified="2013-10-16" version="1">MSMEDNLSSKSQSSASESSEYEIITSSTSKSLNKSPTLNLAKNLDFQELKTVMTEALREEHYIGVKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHU5</accession>
    <name>T1GHU5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHU5"/>
    <dbReference type="STRING" id="36166.T1GHU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003007-RA">
        <property type="protein sequence ID" value="MESCA003007-PA"/>
        <property type="gene ID" value="MESCA003007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075165_6_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF79">
        <property type="entry name" value="CUTICULAR PROTEIN 50CB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="11806" checksum="B5B326DF4D066FED" modified="2013-10-16" version="1">MPFELLHLSPPGRPSPPGGRPAPPRPRGEPGPNDVHEPGMPFDFQYAVNDQPTSNDYSHKANSNGDVTTGEYRVQMPDGRTQIVNYEGEAQFPQGPTGGGRPGTGGGYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI98</accession>
    <name>T1GI98_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI98"/>
    <dbReference type="STRING" id="36166.T1GI98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003167-RA">
        <property type="protein sequence ID" value="MESCA003167-PA"/>
        <property type="gene ID" value="MESCA003167"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712028_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052643">
        <property type="entry name" value="ERP44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46295">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46295:SF1">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577385" description="Thioredoxin domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="32"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003167-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8435" checksum="0189C6D62D2240C8" modified="2013-10-16" version="1">MRVPRFKACIPLVFFLVSFRFMSSKADDAAFKLTADNIDMTLASNELVFINFYAEWCRFSNLLAPIFEEASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIH6</accession>
    <name>T1GIH6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02054246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIH6"/>
    <dbReference type="STRING" id="36166.T1GIH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003255-RA">
        <property type="protein sequence ID" value="MESCA003255-PA"/>
        <property type="gene ID" value="MESCA003255"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_531666_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="9">
        <location>
            <begin position="332"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003255-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="513" mass="59804" checksum="352C4E511A301EBC" modified="2013-10-16" version="1">MEAQQHITKTIEVTRVNLFNIITQYKAIFPDDNQKPQETDGGRIFESWINRRINIFLEVLEEDLSSGISSMDTVLGQCMYFGQSFGRVGADFRGLMVPIFLKVITKNFSTSITKVNQTFRANLEKYTLLNKVNFGLHTRKLDDQKDEISPPETLLDFQPLAFLCNGYLDALNELRLCTPVALVFEITKIFQESLEFVAQQIFYFYRTEKQKFYGSEKENFIKFASCFAYDLIPYVQRCIYAIFPQETVATNLGINLTTVQGEGIGVIQQKTILEPLKELLPNKMDIILRQESALGAGSALLVNKFGGQTLKKCDDNPRALSLNISNVLRNELFKVNRHYETIKELCDENGDFLPEKFNSWFKQLFENEFFPKNEKHSITYNRCTYQYECSFLSSEYFNTMEIKCTNYQIVPYKVQFIPCISMEPNNCIFHNEQIGKWYLIPTEKSENKFQISLFKDENIIYNENKLKIILKVLDKIRVNNEITGLENYHFETVLLWMVKNDEIPKAKQFSNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ41</accession>
    <name>T1GJ41_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003482-RA">
        <property type="protein sequence ID" value="MESCA003482-PA"/>
        <property type="gene ID" value="MESCA003482"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148700_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003482-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12838" checksum="5ED93BDD5C8406BA" modified="2013-10-16" version="1">MSLFTILIQSIIAYKILAFIMKPHIIRNINKYGYTDKWYTNIKASHRRLGQSVTTNLRLCVISFSWGTSLLLRQIIILRKPKILSLTSVNMSPPVGFKAEITHSNNCSERLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ68</accession>
    <name>T1GJ68_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02027067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ68"/>
    <dbReference type="STRING" id="36166.T1GJ68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003512-RA">
        <property type="protein sequence ID" value="MESCA003512-PA"/>
        <property type="gene ID" value="MESCA003512"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_573163_0_0_1"/>
    <dbReference type="OMA" id="IECIKEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF46">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="254"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="477" mass="55610" checksum="5996E88377A0C6D6" modified="2013-10-16" version="1">MLSPKEKMLWSSRSVKDQVVIIGNNSRDEAPRILKDIMENWDPDTNYKSPIRILKDGFESFLLVYPTECTNPHYTSPKVANEGSENLEEIEYPEIGDIMMKDEIKLGSVPEVNRADKSKAMKIYEQRNLNEIIEEKNKLSTENDKTNQEFEEVSKSLRETEQSHDSEEYKKLTFRIWELETEKKNQNYKEQLLIKEIQDLKKMHISKVDDNEKSIIENHKISEPIPKQQVIINEPQIEKLIPVKQDELEDKVLTGLKNLGNTCYMNSILQCLFSSLMLRKFLISNNYKNSINYKSKTKGYICEETAGLFKRMNSGEYKYVECAQLKSYFGEHQKMFRGRDQQDAHEFLTIFIDCLHLELNTIRISRPFSENNSSEKCWNEFTKGEESKILQIFYGQIKSTVKCVTCEKESATHETFSNLSLELPAQAQICLLTDCLDLYFAGERISGWICPKCKQKRDAIKKLSISRLPEILVIHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ92</accession>
    <name>T1GJ92_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ92"/>
    <dbReference type="STRING" id="36166.T1GJ92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003536-RA">
        <property type="protein sequence ID" value="MESCA003536-PA"/>
        <property type="gene ID" value="MESCA003536"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106531_2_1_1"/>
    <dbReference type="OMA" id="PECNSHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF35">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="32"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003536-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15041" checksum="5BE2A8CFD79BEE95" modified="2013-10-16" version="1">MRHIGSRMTLPMERAESFEKALELAKFGKFNILLICTAGIVMANIYLETTSMNVVLPVSQCDLHWTQKERNLLGAVSYIGIIISSHCWGFVGDTFGRKKVLVPTLLVGFVVSVISSFCNSFTCMFIFRLINGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJI4</accession>
    <name>T1GJI4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003633-RA">
        <property type="protein sequence ID" value="MESCA003633-PA"/>
        <property type="gene ID" value="MESCA003633"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661606_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8717" checksum="B2E62EFED2AA25BD" modified="2013-10-16" version="1">DLLASEQSSIIIKSLTKLPKNFNIVVYPCLVTIIYKNEEARKSIAREFNVDFLDEYSKSEKAKKNRLISVLNPDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJK5</accession>
    <name>T1GJK5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02143457">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJK5"/>
    <dbReference type="STRING" id="36166.T1GJK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003654-RA">
        <property type="protein sequence ID" value="MESCA003654-PA"/>
        <property type="gene ID" value="MESCA003654"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064639_1_0_1"/>
    <dbReference type="OMA" id="AYRLMIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048386">
        <property type="entry name" value="Med15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048385">
        <property type="entry name" value="Med15_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31804">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31804:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21539">
        <property type="entry name" value="Med15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21538">
        <property type="entry name" value="Med15_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARC105/Med15 mediator subunit central" evidence="1">
        <location>
            <begin position="20"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="ARC105/Med15 mediator subunit C-terminal" evidence="2">
        <location>
            <begin position="122"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003654-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="250" mass="28383" checksum="A573EC517775FFFB" modified="2013-10-16" version="1">MEWNDCMMAMPWLGKVRYLDSEKIAKINRLLEILLNPQQRIPLETLLKCEKALERMDILHIAPSNKHHFLETVNTVLQTPTGHHTLYRTFHPSFESLFGTDIQMSSIPKKRSMPVEESQETPHVLQGEIARLDQKFKVTLDPATQNQNKTLTLNCCLDDKCLPWVPPICVTIPENYPFISPTCTLNKQEYTATPFLSSVEKSLASRIGKLPKLHSLSHILDTWEMSVRQACSPTSSALTAEDALADILAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP6</accession>
    <name>T1GJP6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dipeptidase</fullName>
            <ecNumber evidence="1">3.4.13.19</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.19" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02079003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP6"/>
    <dbReference type="STRING" id="36166.T1GJP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003696-RA">
        <property type="protein sequence ID" value="MESCA003696-PA"/>
        <property type="gene ID" value="MESCA003696"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181725_0_0_1"/>
    <dbReference type="OMA" id="PDEHANC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF47">
        <property type="entry name" value="DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU341113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003696-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9077" checksum="53AE7FA0056F8498" modified="2013-10-16" version="1">MPNITPKGLEDVSTYPTLFAELLGVGWTMEDLEKLAGGNFLRVMREVERVRDNKKLQGVRPFEDQPDFKSEDPYNCTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJR0</accession>
    <name>T1GJR0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJR0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003710-RA">
        <property type="protein sequence ID" value="MESCA003710-PA"/>
        <property type="gene ID" value="MESCA003710"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402170_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10766" checksum="BADF0EAC1FE66987" modified="2013-10-16" version="1">MPDTKQESSFIFPRTLHSGTSNQTDPRVADRGTLLYPKFIYLNVIFMKTKYTKELSNRDLGLIFSLLKYSGLVVHNYYSSILYVQPSNIVREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK15</accession>
    <name>T1GK15_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK15"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003826-RA">
        <property type="protein sequence ID" value="MESCA003826-PA"/>
        <property type="gene ID" value="MESCA003826"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323042_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003826-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="10556" checksum="2E61C4F20316A9A2" modified="2013-10-16" version="1">MLMKCTNNKSAYGLKITVLVVKDNKIALSSNKKTYPNLRMLGLVHVAAAPGSVQEQKEGGCIQRIFLSKSNKKFSSDAFSTSMLQIHITGPAGSVGGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKB6</accession>
    <name>T1GKB6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02076827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02076828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GKB6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003936-RA">
        <property type="protein sequence ID" value="MESCA003936-PA"/>
        <property type="gene ID" value="MESCA003936"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1637352_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003936-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="17958" checksum="0050787C86BB5694" modified="2013-10-16" version="1">MFLIVYNESCGPTTVFSLIFSMLYALFAANCKSGLRNLQIPYIRATNKLDFTFLFLATWMDILTILSACAALARTVSSCLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGLARIFILGRNSPTNEPWPDVLGVMVVFIVTGMFMLGLEVSISIIFKIDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKG3</accession>
    <name>T1GKG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKG3"/>
    <dbReference type="STRING" id="36166.T1GKG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003986-RA">
        <property type="protein sequence ID" value="MESCA003986-PA"/>
        <property type="gene ID" value="MESCA003986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357667_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="61"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="23133" checksum="E8E41595CC1A84A1" modified="2013-10-16" version="1">KEFIFLSIPDVRPGLIKDRIKLRENEIKSKQKVYSEKQKQALKKPDFKEQLEAGLSSEISESNKGFAMLQKMGYKKGDSLGKSSTEGIKEPIAIKIKENRSGLGVEAKRLEDEEKKKQLREQILKRKKESSENNRIKLRENEIKSKQKVYSEKQKQAFKKPDFKEQLEAGLSSEISESNKGFAMLQKMGYKKGDSLGKSSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKI3</accession>
    <name>T1GKI3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GKI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004007-RA">
        <property type="protein sequence ID" value="MESCA004007-PA"/>
        <property type="gene ID" value="MESCA004007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_566557_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="4">
        <location>
            <begin position="292"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="419" mass="46325" checksum="F7AA62FA8FFC2E65" modified="2013-10-16" version="1">MVKTEPVDGNHKMDVEFGESSNSNSTFIPPVKRRKSDQNNGAGMIVGWGGNTSGPGNAENGELSESSVGHSSISGLPRKKNSVRSQLAQQILNSSTRTFKKPMYAVRPAPLTNQQLYPQTQSANSAYDPTALKAIFRYLPPETLVTCSAVCKVWSNVSVDPDLWKVMNCAEHKLSASLLTAIVRRQPEQLVLDWTQIAKRQLSWLIQRLPAMKHLSLQNCPIQAVPALHTCNCPTLQVLDLSFVRGLNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPKDSRPGLTDSKSRLRGLKRLYIAGTDISDVALRYITQGLQQLVHLDLSSCQRVTDAGIAQIGTCQNAINTLVELNLSGCRLVTESSLDYLSKCEKLIWLDLRHVPNVSTQSVIKFAAKSKHDLCVKDIKLVERRNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKP1</accession>
    <name>T1GKP1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02125997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004068-RA">
        <property type="protein sequence ID" value="MESCA004068-PA"/>
        <property type="gene ID" value="MESCA004068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069908_3_0_1"/>
    <dbReference type="OMA" id="CWFEPSN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF25">
        <property type="entry name" value="IP09473P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="266" mass="30918" checksum="193D9C2F80CA25CE" modified="2013-10-16" version="1">MNIQSNLSIQYFNQCCYFEKKKVPQKSKTNNMLFFAADYIKQCEIAQPVSVFTKCSTQSIQQLFNKLDEGIPGLTSVESFDPFYLNKIKINQGNNQAINLKVELSKIKITGFGKTVVKESRVYPHDYSWKTTFVLPNMKLDENMTVIMYTKTRLYEKGGFTFYNVTNVRVDFKLDGLKSHFSNLFNGNKALEESTNRFFNENWRMLADALYTVITHTIEDILIDVLQKIFHFIPANFFVSDIPTSEELYSRQKNSNTKVQQKQKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKZ3</accession>
    <name>T1GKZ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA-binding protein 15B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM Spen family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02059143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKZ3"/>
    <dbReference type="STRING" id="36166.T1GKZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004176-RA">
        <property type="protein sequence ID" value="MESCA004176-PA"/>
        <property type="gene ID" value="MESCA004176"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012724_1_0_1"/>
    <dbReference type="OMA" id="EWPNPAI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12308">
        <property type="entry name" value="RRM1_Spen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12309">
        <property type="entry name" value="RRM2_Spen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12310">
        <property type="entry name" value="RRM3_Spen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21544">
        <property type="entry name" value="SPOC_RBM15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000007">
        <property type="entry name" value="RNA-binding protein 15B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000454">
        <property type="entry name" value="RNA-binding protein 15B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000565">
        <property type="entry name" value="RNA-binding protein 15B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010912">
        <property type="entry name" value="SPOC_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50917">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="285"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="366"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="SPOC" evidence="9">
        <location>
            <begin position="568"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="439"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="494"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004176-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="85199" checksum="1AFE717B25C96433" modified="2013-10-16" version="1">MSTRESDRITVKIHNMKRSGSRERDSSRSKRRGSFSRYDGSSDEGEPDRVRRRVIRSPSPRTHSPREDYHERGSERPTYKVLCVSALHAKASEEYIKETLYREYKQFGDFSIRISHDLDERVAYVCFRTPEDAREAKHSKPRVVLYGKVALVEPVYEHGIREYDRYHYTRSSPSTDSHSRRRAPTSSEHHYERYPISDPLHPHPRFRPPIHHHEGYLPPPPHGPPYIPRHPHHIHHPPHPYMHHPRPIIPRPHHPGSYEKAENKKDKFPNYLHHVQPEEDPLAARTLFAGNLEINISDEELRRIFGKYGIVEDIDIKRPPPGTGNAFAFVRYQNLDMAHRAKVELSGQYIGKFQCKIGYGKATPTARIWLGGLGAWTSVTQLEREFDRFGAIKKIEYQKGDTCAYILYESVEAATSAVKEMRGFPLGGPDKRLRTDFAEVAGQPTPAPYKKSYDEDGTTTSTTSEYRRPEYDGYHERYRSRGGYRGRGSYRGMYHNEGHRLPHPPIPREDDWRPPPPPHDRDYHDHRGRSGSREPDSKHRSPRGRRSPDSDSDSSSRRNGVLSSAKTFSDITKKIPVHWQGALILKSSLFPAKLHLTDGDADIVESLMKDENDRHNLRITQRLRLDPPKLDDVQKRMNSSSSHAIFLGLAASTSSVTSEDSGVQTRPLRNLVSYLKQKEAAGVISLLNKETEATGVLYAFPPCEFSTELLKRTCHSVSEEAMKEDHLVIVVVRDSSVFDIDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL76</accession>
    <name>T1GL76_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondria-eating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Spermatogenesis-associated protein 18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MIEAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02038753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004269-RA">
        <property type="protein sequence ID" value="MESCA004269-PA"/>
        <property type="gene ID" value="MESCA004269"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500506_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035694">
        <property type="term" value="P:mitochondrial protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035695">
        <property type="term" value="P:mitophagy by internal vacuole formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026169">
        <property type="entry name" value="MIEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031981">
        <property type="entry name" value="MIEAP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21771:SF1">
        <property type="entry name" value="MITOCHONDRIA-EATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21771">
        <property type="entry name" value="MITOCHONDRIA-EATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16026">
        <property type="entry name" value="MIEAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondria-eating protein C-terminal" evidence="13">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004269-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="10331" checksum="DD07B65BBBCC71FB" modified="2013-10-16" version="1">MLQVLSTLHEYPCLETCTPLVHYISASVRLAWKMINQKVPYYLDNDFNLGFLRPDKHERHFQGDRRSDLIKNFIWPALMQNNNFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLG4</accession>
    <name>T1GLG4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02039024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLG4"/>
    <dbReference type="STRING" id="36166.T1GLG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004366-RA">
        <property type="protein sequence ID" value="MESCA004366-PA"/>
        <property type="gene ID" value="MESCA004366"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_680250_0_0_1"/>
    <dbReference type="OMA" id="TKESPEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004366-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="405" mass="44964" checksum="C18F6B7F5AAA5282" modified="2013-10-16" version="1">MKINPTESLEKQYQRYLNDKAVKKQDKYFLDQAGKNEIDFENMTYKSKGNNVTIMRTSLPGVWISLKTSPNQTLLHAKINRLQCDNQLFDCIFPVVLAPIPPPPSVGDSETLKPFLECSVVKRNVPNSTVQQYKYASILIQEFHIKVDLMFLNALAEIFSGETTDDSATKLLIQDVESIEKPLLDLVSSKSEVEQKNFYDNLHLGPLKIHISFSMAGSENIQLPALIGVLVQSVGVTLTDVNDVVIKLAFFQREYQFYSKSQVISEISTHYTGQFLKQLYVLVLGLDILGNPYGLVVGFTKGATDLFYEPFQGAIQGPSEFAEGLKIGIHSLFSHTIGGAAGAVSKMTGALGKGLAALTCDEDFQKKRRQQQQRKPKTCSEGLARSGKGLATEYTKESPECSQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLN3</accession>
    <name>T1GLN3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02062300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02062301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02062302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004445-RA">
        <property type="protein sequence ID" value="MESCA004445-PA"/>
        <property type="gene ID" value="MESCA004445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852253_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7621" checksum="E4C60D6E0FCAFDA9" modified="2013-10-16" version="1">MRFQPFTHDECKDLINDKYLHGNQQLEIVNSGRYTVVSWIILKDREQQGKADEIGIKPLICLLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLP7</accession>
    <name>T1GLP7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLP7"/>
    <dbReference type="STRING" id="36166.T1GLP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004460-RA">
        <property type="protein sequence ID" value="MESCA004460-PA"/>
        <property type="gene ID" value="MESCA004460"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2984990_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004460-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="58" mass="6254" checksum="2C36EB5AC19E564A" modified="2013-10-16" version="1">MCVLVHLMSPLEPIDPILESPLANSLLKQRAVAAELLNEVQQANTAIGGMLLANMEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLS9</accession>
    <name>T1GLS9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004493-RA">
        <property type="protein sequence ID" value="MESCA004493-PA"/>
        <property type="gene ID" value="MESCA004493"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811019_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004493-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="17265" checksum="C8977DDBCAFB372B" modified="2013-10-16" version="1">YYDFEVVNNFLYLGTERQQPHSRDKTKVKFYPLIPVLLYGNKFYSIRSWALKDSDEELLALFERILRIIYGPVCEEDKRRIRYNQELYQLYQHPSNCTNCINIQTLFENFELAVCNGLEMCKGWKKMSRQENVSLQTRMDIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMY8</accession>
    <name>T1GMY8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMY8"/>
    <dbReference type="STRING" id="36166.T1GMY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004925-RA">
        <property type="protein sequence ID" value="MESCA004925-PA"/>
        <property type="gene ID" value="MESCA004925"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_17_0_1"/>
    <dbReference type="OMA" id="YEHSAIE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="206"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004925-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="33648" checksum="A089F0A81F4EE554" modified="2013-10-16" version="1">MGMTSLLQSILNNSRRIISQRPFDEEYLLDLVEDYQVTRVYTTVTEFTRLANAISLPSRNLKSLRIVSVGGSYVCSELIMRLQKYLPNGVIVINYTITEFEGIAVQNPDAKINPKSVGKPMHNTFVKVIDSLGQDLGSEKTGEICLKRTDKVFGGYFNNILETEKVIDGWLITGDIGFYDNDGYLYILGRKDEIYTYEGCVFYPTQIEERIYRLKDVREACVLGLKESDENYVPAAVIVTSEKSTLTELDVMMEVMKDMDDCNRLRGGVFFVDEIPKTRNGSYCRKTVSNFIVKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMZ8</accession>
    <name>T1GMZ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMZ8"/>
    <dbReference type="STRING" id="36166.T1GMZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004937-RA">
        <property type="protein sequence ID" value="MESCA004937-PA"/>
        <property type="gene ID" value="MESCA004937"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187010_1_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF13">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004937-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7322" checksum="5E200DC7F61739D6" modified="2013-10-16" version="1">MALVVEKISDRYLLHALDRFWHNSCLKCHCCNRLLADLGTSCFSKGGYILCKKDYSRWFYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN54</accession>
    <name>T1GN54_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Activin types I and II receptor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02141056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN54"/>
    <dbReference type="STRING" id="36166.T1GN54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004996-RA">
        <property type="protein sequence ID" value="MESCA004996-PA"/>
        <property type="gene ID" value="MESCA004996"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_785939_0_0_1"/>
    <dbReference type="OMA" id="HIDCCHG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070724">
        <property type="term" value="C:BMP receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23600">
        <property type="entry name" value="TFP_LU_ECD_Sax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF72">
        <property type="entry name" value="RECEPTOR PROTEIN SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588418" description="Activin types I and II receptor domain-containing protein" evidence="13">
        <location>
            <begin position="21"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Activin types I and II receptor" evidence="14">
        <location>
            <begin position="71"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004996-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="353" mass="40853" checksum="C70AC09960DE1E38" modified="2013-10-16" version="1">MANKKILFLFLIYLTKCIFAEEMSPEALVADMEPNDDILQNQSQNQNQQQQQIPYHNKEIASQNSIFQKRYKCYSCDSPCKNATQHAHRCTNALQCWKSRTRDIYGQELVSRGCTTSSDVLPFCSRRNDGQRKRHTSGQYHIDCCHGDYCNDGDFPDLPMAPDPNNDIEKRASPEHDSKFNYTVLITSFALIICITIAALYFFRRKHRKRLLATRSKQDPETYYASDDLIRATSAGDTLRRADIYALGLIFWEVCRRTTSCGIAEEYKIPYYDVVPSDPSFEDMKKVVCHDNYRPSIPNRWIVSDPLLGGMAKLMKECWHQNPSVRLPILRIKKSIQKLADSDKFQLKFDENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNN8</accession>
    <name>T1GNN8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NHR2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNN8"/>
    <dbReference type="STRING" id="36166.T1GNN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005196-RA">
        <property type="protein sequence ID" value="MESCA005196-PA"/>
        <property type="gene ID" value="MESCA005196"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1012970_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013289">
        <property type="entry name" value="CBFA2T1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014896">
        <property type="entry name" value="NHR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379">
        <property type="entry name" value="MTG8 ETO EIGHT TWENTY ONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379:SF14">
        <property type="entry name" value="NERVY, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08788">
        <property type="entry name" value="NHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NHR2-like" evidence="2">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005196-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="275" mass="30416" checksum="39A61F8DD5020C93" modified="2013-10-16" version="1">MEFSHLPPDHSEIFLPIEPPTTTPTSSSLNGNGVVFKRRPSDTLIDHHNGGAEWPDYLYPPAKRHHPHPSHLSSSVSVGGRDGAGAGPTNYSTQPSVYDYPSSVVNRGDSDPGGFSVLLDKSRDERDLQRAAVASANAASAAAAAAEVQRLNRMSGEEEWKNIHTMLNCISAMVDKTKRAITILQQRGVDNQPNYPNEPSAVAVAEIRRQTEEKVAEFKRNAEDAVNQVCILMKGFSFNNTCKRFTITQRKDYGIMLRDVYYILFNIIFSQQAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP07</accession>
    <name>T1GP07_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">1-phosphatidylinositol-5-phosphate 4-kinase</fullName>
            <ecNumber evidence="11">2.7.1.149</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:55993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + GTP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:55965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12280"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.149"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:12281"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.149" evidence="11"/>
    <dbReference type="EMBL" id="CAQQ02166772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP07"/>
    <dbReference type="STRING" id="36166.T1GP07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005321-RA">
        <property type="protein sequence ID" value="MESCA005321-PA"/>
        <property type="gene ID" value="MESCA005321"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004312_7_0_1"/>
    <dbReference type="OMA" id="DYSPMCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:1-phosphatidylinositol-5-phosphate 4-kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17305">
        <property type="entry name" value="PIPKc_PIP5KII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 5-phosphate 4-kinase type-2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="14">
        <location>
            <begin position="23"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="277"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005321-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="395" mass="46427" checksum="5AA7EC3CF8A802D3" modified="2013-10-16" version="1">METRILKKKHFRVKHQKQKLFRANEPILSVFMWGINHTINELSHVNIPVMLLPDDFRANTKIKVDNHLFNKDNMPSHFKEYCPLVFKNLRERFQIDDVDYRESLTRSQPILIDSSGKSGAKFYQSYDKFFIMKSLTSEEVERMHAFLKHYHPYVVEKHGNTLLPMYLGMYRLTVENVQYYFVVMRNVFSAHLTIHKKFDLKGSTVDREASDKELEKNLPTFKDNDFMSQRKKIQIGQDAKEKLFQTLQNDIDFLKKLHIMDYSLLVGIHDKKQAEEEELTAQNENVEGNRSDHSESEDGERWPHYKTPPDSPHQGQCHGNYNDIIPEIDLYALGSADQKEIFFVAIIDVLTQYDVKKQAAKAAKTVWYNNVDGISTCDPEQYAKRFIEFMDKVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPH8</accession>
    <name>T1GPH8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPH8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005508-RA">
        <property type="protein sequence ID" value="MESCA005508-PA"/>
        <property type="gene ID" value="MESCA005508"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870157_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005508-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7066" checksum="0A355C41A84087A8" modified="2013-10-16" version="1">MFKQLNSCGLGLRPVYRSNVQQECYECSSNLCNANSISPNNGSSSLSFGYLYIFVVGVLSKMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPJ2</accession>
    <name>T1GPJ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPJ2"/>
    <dbReference type="STRING" id="36166.T1GPJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005524-RA">
        <property type="protein sequence ID" value="MESCA005524-PA"/>
        <property type="gene ID" value="MESCA005524"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388718_0_0_1"/>
    <dbReference type="OMA" id="MLYIQAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005524-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10487" checksum="F69A4E23E0A7FB67" modified="2013-10-16" version="1">MEKNNCQHIPSSPHYSQSNGEAESAVKTAKLLVEKGEDILDALLEYRSTPLSNRFSPAELLMGRKIKASLPTCPRNLETTLSKIVCDKEKELKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPR5</accession>
    <name>T1GPR5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPR5"/>
    <dbReference type="STRING" id="36166.T1GPR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005606-RA">
        <property type="protein sequence ID" value="MESCA005606-PA"/>
        <property type="gene ID" value="MESCA005606"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1236298_0_0_1"/>
    <dbReference type="OMA" id="YWITELL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005606-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25095" checksum="96F2F0C4FA892985" modified="2013-10-16" version="1">MEEIYFSDLTTRQRCFKFLRKHWKGIFIIVWPILCSPILLVTDGGKALRCGFLILLMSGFWITECIPSHITSIMPIVFLPALGILSSQITCENYFKDTIIMFMCGIMIALLIEFTHLNMRIAFVAVKLVGPSPRRINFAMMVLTGFISTWISNTAATAMMCPIIKGILSSLGSHGIDIYEAKDVEANVPIKKRRPSKLAIAMYVSTCYAATMEDVGQSLVPQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQC4</accession>
    <name>T1GQC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02042342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQC4"/>
    <dbReference type="SMR" id="T1GQC4"/>
    <dbReference type="STRING" id="36166.T1GQC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005828-RA">
        <property type="protein sequence ID" value="MESCA005828-PA"/>
        <property type="gene ID" value="MESCA005828"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1373646_0_0_1"/>
    <dbReference type="OMA" id="NWHLASS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF26">
        <property type="entry name" value="ODORANT RECEPTOR 10A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22807" checksum="7F464176934F39CA" modified="2013-10-16" version="1">MSEMGRSLAGETLSTKRNLQQESRKSPYKDLCLTLFIKEFLSYDQNTNLIRELSKIIQSHNDLITLTQDFAHLFTIIILGHFVTSGFVLCVSCLNFILVESYLLKAIYLFYNAVVITQLFIFCSGGASIVDTSTEFAETAYFTEWYKCDIRVRKFIWLIIMRSQKPIELDVPFFAPSLPLFTAICRTAGSYIALLKTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRL4</accession>
    <name>T1GRL4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRL4"/>
    <dbReference type="STRING" id="36166.T1GRL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006300-RA">
        <property type="protein sequence ID" value="MESCA006300-PA"/>
        <property type="gene ID" value="MESCA006300"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1410310_0_0_1"/>
    <dbReference type="OMA" id="HTCMENG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF60">
        <property type="entry name" value="GRAM-NEGATIVE BACTERIA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH16" evidence="1">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="22284" checksum="71BA5664F9DCE1E7" modified="2013-10-16" version="1">MPSADWVFPQIFLNPVDHKYGKIDYASGQLRVAQSVGNCKLKAGAILNVKEPFRSVKTCIHTCMENGDWHNDFHNYTMLWLPDSIKFFVDGNEYCSVDEGEGLYAVQANGRDLPSKDSLKTLGDSLLAPFDKDFYITLGYGVGGIYEFDDNSRKPWRNDDARSLNKFWRNAKKYDWTDDKYKFEIDYIRVYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRY3</accession>
    <name>T1GRY3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDAH family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRY3"/>
    <dbReference type="STRING" id="36166.T1GRY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006430-RA">
        <property type="protein sequence ID" value="MESCA006430-PA"/>
        <property type="gene ID" value="MESCA006430"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148715_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016403">
        <property type="term" value="F:dimethylargininase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000052">
        <property type="term" value="P:citrulline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045429">
        <property type="term" value="P:positive regulation of nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033199">
        <property type="entry name" value="DDAH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737:SF9">
        <property type="entry name" value="DIMETHYLARGININASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737">
        <property type="entry name" value="DIMETHYLARGININE DIMETHYLAMINOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006430-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12373" checksum="9AE9261D954E767D" modified="2013-10-16" version="1">MAPKYTHAIVQRISSSLQEIGDIDLEIAKKQHEAYVNILREIGLDVIELPPDDELPQGIFVESAAVICNGVALIARSKDTTRQKEILLHFYSLSGYKLRLPTGPFGKTSPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS44</accession>
    <name>T1GS44_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132809">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS44"/>
    <dbReference type="STRING" id="36166.T1GS44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006492-RA">
        <property type="protein sequence ID" value="MESCA006492-PA"/>
        <property type="gene ID" value="MESCA006492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_824620_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="110"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="337" mass="38769" checksum="8348D4D1734F1B83" modified="2013-10-16" version="1">MSKAEGSYISFDILRRVLSDYFGYNIHYVMNITDIDDKIIKRARQNYLYEQYVQEVGTKSLEDVLKDQKEVLTAFQATCDKNTDPDKKVMLDKMLEKMMKAVDSLTKSVESGNNEAILQAKEFYLKEAKDPIAEWVDKQKGADVTENSIFESLPRFWEDEFHKDMTALNILPPDVLTRVSLREYALTSALRDSRFSPITREEFPKLTVSVSILQNFEEARGFLDWSLGIHGIRIEFLNERGMKRTATYLPQVATEQGWDQIQTIDSLLRKGGYKASITPEVRKSIKLTRYRSQEIQMNYNEYRSVVEQQQQQKRGNFNGNSGTGGSANSGQLGRVQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSD9</accession>
    <name>T1GSD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSD9"/>
    <dbReference type="STRING" id="36166.T1GSD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006594-RA">
        <property type="protein sequence ID" value="MESCA006594-PA"/>
        <property type="gene ID" value="MESCA006594"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_767893_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06526">
        <property type="entry name" value="metazoan_ACD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="SHSP" evidence="7">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="147"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006594-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="40599" checksum="AD9AC3B51D57FF3B" modified="2013-10-16" version="1">MSSQLEIRHLGHTAPILAAEPFFCMVTMDLRHYKSNEVEVKTLHDTIVIEGAQKSDENNDKTRTFCLRPKFLRKYKLPRYYDPSRTTATFSSDGILIVTVAAPAPPEITENIIKIEDVGPFFESSKAKAIVLTAFTFNYCSCSREHITTPQLPNEETFKINIAKPGSAPYQVSLQTLSGSHFCGGIISDERRIITVAGCLDSRKPSDFKILAGSQNLNDRNGTYFEPEEVNIHNNEKISIEIWRQINIIGLEVGLCSRKRIADEELQSLEVEYKSYGDCQYLQQNSTLIDYGGTCTFKNLRKEACLGDSGGPLTYNGKLVGLVNWGRPCALGYPDAHARISYFHDFKTPPPFQILHIYNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUL0</accession>
    <name>T1GUL0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUL0"/>
    <dbReference type="STRING" id="36166.T1GUL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007425-RA">
        <property type="protein sequence ID" value="MESCA007425-PA"/>
        <property type="gene ID" value="MESCA007425"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_393948_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007425-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="700" mass="81058" checksum="EEAB37224203A657" modified="2013-10-16" version="1">MDREARKFIWKLDGERLAELCRELDLDIDDSAPTAMKLKIGEMASNATLEQFEILKRWSEKEKENKDKLNIHKWVYGMSRKECSQLLRQVQIPVEGAEFEQKNALIKFFNSSTGVVREGFVEMAEDFLVNQNVLPSSTRNNHKSEHSYDSNQSRRSRTLRHQGRNESFNPHPHGHDSVQEKHSHKEGRGFQKKCKRPDKRMSCEESSNSESSETAETESHYRPERESIVTRRWSTAQLMDTVRKWNIYFSYTEKPQDAITFLDRVKERAECYKISKDRLPKAMPEFLRGQALEWYRNNNEEWRTWGSFTESFTSFFVPPKMKLQFQDDVTAYLQKQDQKEPPTLEWCQLCRRRVPSAENCGCKQTGTSQSRKDQSIASASNVNEIQSDTRPHAVVELEGRRFIALLDTGSTASYINGDVGTFLKECGVLKGSMSSNIRLADGSVKLLSDFFELDITLFGNRMRHKFTIMPTLSESILVGDDLLKKNNVHLRDDFGNIVGTEIDKQVVMEIMNEDIQESSPICAMENISNRNQNVSNKKGKLNVVPDTLSRDAIDENEVFSELCNFVEEDLEDCAWIKNKKKAIQTHPEKHSEYMIENGRLFRNMGCGLTNMAKASVMQAKYYNLRRRDWRPEVGELVYKRNFPQSNAQNAYAAKLAPVFSGPFRVLNYISPTIVELKSLDINDKRIRKIHLKDIKRIDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVC2</accession>
    <name>T1GVC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cullin neddylation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVC2"/>
    <dbReference type="STRING" id="36166.T1GVC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007714-RA">
        <property type="protein sequence ID" value="MESCA007714-PA"/>
        <property type="gene ID" value="MESCA007714"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798644_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin neddylation" evidence="1">
        <location>
            <begin position="89"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007714-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16634" checksum="87EAD66548DAD90F" modified="2013-10-16" version="1">MTILLLFENVNSFTCKELQDSLQLNTDSLQKHILPLIESKILLATSEFFSDNTIISLNLEYSNKRTKFRITAALQKETPQEVEHTMTVVDEDRKLYLQAAIVRILKSRKVLKHNALIQEKITVNVVHSIECKENLFCNNDNNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVP2</accession>
    <name>T1GVP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-glucuronosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVP2"/>
    <dbReference type="STRING" id="36166.T1GVP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007855-RA">
        <property type="protein sequence ID" value="MESCA007855-PA"/>
        <property type="gene ID" value="MESCA007855"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012949_2_0_1"/>
    <dbReference type="OMA" id="SFKERTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007855-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39258" checksum="1854F6F511CA5674" modified="2013-10-16" version="1">MGLVAPWSNAPHFMLPYDDNMSFKERTHNAVLTIYDLFYRNFYYLPQMQSMVNKHFANISNEPLPTIQELEKKVSLILVNSHRGTDSPRPTISGLVNVGGAHIKKPKPLPEDVRKFIESAENGVIYFSLGAFMKSADMPKAKLEIIIKALSQLKQKVLWKYEDESLTNIPKNIMIRKWMPQNDILAHKNVVLFITHGGRFGTLEGTYWGKPMLAIPLFGDQHTNVQRLKRQGIALSLDFLTFKSEELVSKVNTLLSKESVDRSKTISTLFQDNPIHPMDEAMYWIEYVIRHKGANHLKSKAINLNVFEHLLLDVFFIHLAFVVFAVVFLYSILSKVFGLYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVR3</accession>
    <name>T1GVR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Voltage-dependent L-type calcium channel subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02090988">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GVR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007879-RA">
        <property type="protein sequence ID" value="MESCA007879-PA"/>
        <property type="gene ID" value="MESCA007879"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000540_0_2_1"/>
    <dbReference type="OMA" id="CMQNGTK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009582">
        <property type="term" value="P:detection of abiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009581">
        <property type="term" value="P:detection of external stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050906">
        <property type="term" value="P:detection of stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042045">
        <property type="term" value="P:epithelial fluid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000009">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000107">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000001">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000006">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000064">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000095">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031649">
        <property type="entry name" value="GPHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014873">
        <property type="entry name" value="VDCC_a1su_IQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005446">
        <property type="entry name" value="VDCC_L_a1su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF1">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE D SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08763">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16905">
        <property type="entry name" value="GPHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01630">
        <property type="entry name" value="LVDCCALPHA1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01062">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 17" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="10 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="603"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="759"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="829"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="876"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="990"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1072"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1102"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1132"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1230"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1323"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-dependent calcium channel alpha-1 subunit IQ" evidence="20">
        <location>
            <begin position="1481"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="641"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="665"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007879-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1686" mass="191314" checksum="5C09262821CF5F36" modified="2013-10-16" version="1">MLQNLALNGVCTSANLETSRTQQTFEYIILTTIMANCVALAVYTPFPASDSNTTNAALEKIENIFMVIFTSECVIKIIANGFLMHQGAYLRNGWNILDFFIVVIGAISAALSSVMPEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMIPLFHIALLVLFVIIIYAIIGLELFSGKLHKTCRHNVTGDYMEDPGPCGVGYQCEPPYQCFDRWEGPNFGITNFDNFGLSMLTVFQCITLEGWTDVLYDVDPRLNGKYLAWIYFVSMVILGAFFVMNLILGVLSGEFSKERTKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIDPDVDQQLDLQAKNYMDDNLMGDTDSQKHSAMGEECETIVQETSWTRFKKRMDRANRRLRRSCRKAVKSQAFYWFIIILVFLNTGVLATEHYRQPPWLDEFQNNTNLFFITLFTCEMILKMYSLGIQGYFVSLFNRFDCFVVIGSISEMVLTNTGIMPPLGISVLRCVRLLRVFKVTKYWHSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGRFNFNKEEAKPRHNFDSFWQSMLTVFQILTGEDWNVVMYDGIRAYGGVHSPGVIACIYFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKEEEAPAEAIGEPVIDGPSSVLEMEDENSPNMEEEDERGGSQGDVDLIDDMEANENAEEADDYYDEEEKIGARPRRMSEYNNPGTSIVPIPAGSSFFILSQTNRFRVFCHWLSNNSYFTNFMLFCIMFSSGMLAAEDPLDGKSERNLILMKFDYFFTTVFTIELLLKVIAYGLVLHQGAFCRSAFNMLDIVVVFVSLISVFSSSNAIATVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLEFMFAVIGVQLFKGKFFSCTDGSKVKEEDCKGTYLIYENGDVTKPRLKEREWDVNDFNFDNVGRAMLTLFTLLYVSIDSNTEDEGPIHNFRPVVAIFYIVFIIIIAFFMVNIFVGFVIVTFQNEGEQEYKDCDLDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQGFEYTIFVLIMINTVTLTMKFYKQPDYYSNFLDALNMLFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKVSEGFKAPVIKEGSGKQNIKPSTGDIDIQNFKPHKASGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVIGMQVFGKIKLDPDSSLHRNNNFQTFPQAVLVLFRSATGEAWQEIMMDCSVRPEKVVCAPNSDDPDNTNCGSYVSYPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGHIKHLDVVTLLRKISPPLGFGKLCPHRVACKRLISMNMPLNSDGTVMFNATLFAVVRTSLKINTEGNIDDCNAQLRATIRKIWKRTNPKLLDQCCPPPGDADEVTVGKFYATFLIQDYFRRFKKRKEQEGRDYQGDEATITLQAGLRTLHDAGPALKRAISGNFDELLEEPEPMLGVITVYLVVCGHRYVDEAKILLEGNGKPGVGDIIASIKPDADSLANERPYPISMLSAQRPGRFVPARPVALSVSSAIGPAESLVGMVLTEQGLGKYCDTEFVKTTSREIQEALDMTQEEMDRAAHQLLHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW16</accession>
    <name>T1GW16_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein krueppel</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW16"/>
    <dbReference type="STRING" id="36166.T1GW16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007984-RA">
        <property type="protein sequence ID" value="MESCA007984-PA"/>
        <property type="gene ID" value="MESCA007984"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_94_1_1"/>
    <dbReference type="OMA" id="ECTHTEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="12">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007984-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="38566" checksum="44555F2EBCCEF17D" modified="2013-10-16" version="1">MQKTTNECRVCLENVRNFKEFTEILICDTEPQESILSIFEYSTSIDCSNYLEEGPMFLCISCIKNLKFVYDFIRQARESDEKLRNSSEIVAKPTEEIESVCEDEETSEFLETDTTSEIIYVTQPIESVAEYHSPDIIEEEFVLRAESESFLESLNEEENFMEHVAEEKTQVQQETNEKRICKRKPYVPRKDIPANFNCVHCDKNFVHNFLLKAHIRQYHEPGSKERNWQCSKCDKKFFKKEVLSRHLLTVHREGRDFKCNICGKEFQYKERLTRHLRIHIDRKFQCHICDKSYVQNADLNVHMRTHTNGSHSNVTNVRKHSQISPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWL7</accession>
    <name>T1GWL7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">I/LWEQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02110959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008194-RA">
        <property type="protein sequence ID" value="MESCA008194-PA"/>
        <property type="gene ID" value="MESCA008194"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_401514_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="FunFam" id="1.20.1410.10:FF:000001">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
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        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054060">
        <property type="entry name" value="TLN1-like_RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015009">
        <property type="entry name" value="Vinculin-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981:SF1">
        <property type="entry name" value="RHEA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981">
        <property type="entry name" value="TALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21865">
        <property type="entry name" value="TLN1-like_RS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08913">
        <property type="entry name" value="VBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="I/LWEQ" evidence="5">
        <location>
            <begin position="320"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="160"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="557"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="573"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="608"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="656"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008194-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="686" mass="74334" checksum="C9AF9455147689B8" modified="2013-10-16" version="1">MVNGSIGACTHIIHSNQQIMLINQTKSVVESAAQLVQSAKESGGNQRATDAHNNLDESIENTREVIKDLTQTVEKLSAESGIVTGLMEQVNRSIARLTDKRQSLLAGSVNINDTFVDYQTRMVQHAKEIARLANEMNVNSHDGSKLPQLGLEMTQHYSKLTQDSMGASTTTSSPEVGFRIRDSSPIPPQSPSFLATQQKLNSSSSPQTVRPPPNPKLTGNSIPRPYPGSPTMSRSTGIGNSLTGGVGAGDQSPKPSQSFDKSSTFPSINNNEQKVARNLEACVQDLHDKTFGQGSVVMTNRRVAKSVTDLVALARLLKGSDWIDPEDPTVIAENELLGAAASIDAAALKLKSLRPRRQDDAKIELDQNMNFDTLILTAAHSIMTASAALVRAANAAQRELIDQGKVERKPLSSSHDGQWSEGLISAARFVASAAHSLVEAAQKLVVGQGTEELLISSAKQVAYSTAQLLIAFKVKGNPNSEAGRRLQMAGNAVTKATDNLVKAAQQALDAEEEHSIKIHTSMVDGMAQEIDARSEEGGXXXXXXLEEARLKLKRIQQAKYHNRKEEDSDSDYHNNSLNRSGVYNQNNSHNVVNQSPTMLSYQNQQQQNPPPPVPPLPQQYPPVRTFQSPPQHFHYPDAVNPTYTPVNKSPIPPQSPSFLATQQKLNSSSSPQTVRPPPNPKLTGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWT1</accession>
    <name>T1GWT1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02122551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWT1"/>
    <dbReference type="STRING" id="36166.T1GWT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008261-RA">
        <property type="protein sequence ID" value="MESCA008261-PA"/>
        <property type="gene ID" value="MESCA008261"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_6_1_1"/>
    <dbReference type="OMA" id="STITEDW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="14"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="287"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="310"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="331" mass="36279" checksum="BB5C9AF7B007D6ED" modified="2013-10-16" version="1">MCLKAFVSYFAEAVPNYEGFSIQDFQDKTQNILSKPILEYVMKASLRKSTEQHNRDAFKNIYFRPKYFKDVSNLSLTTSLLDKEMSIPVGIGPSAMHKIAHHQGEVATALAAGSQGTIYVLSSFSTTSIEEIGEKAPNTEKWIQMYIYKNLSINLNFIRRAEKANFSGIVVTSDAPVHGKTYLKYEFDLPDGLRMENFVGITDRPSQIVSSSFTIKNLKWIVNATKLPVFAKGILTKEGAIKARDAGCKGVFVSTHGGRQLDGVPASIEALPEVVKAVGKDMTVVFDSGVRGGDDVFKALALGAKYVFIGRPVFFGLAVDGQKGVEGVLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWY8</accession>
    <name>T1GWY8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone deacetylase complex subunit SAP130 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP130 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02035080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008323-RA">
        <property type="protein sequence ID" value="MESCA008323-PA"/>
        <property type="gene ID" value="MESCA008323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_335756_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024137">
        <property type="entry name" value="His_deAcase_cplx_SAP130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031963">
        <property type="entry name" value="SAP130_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13497">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13497:SF3">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16014">
        <property type="entry name" value="SAP130_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP130 C-terminal" evidence="8">
        <location>
            <begin position="544"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="538"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="626"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="650"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="677"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="850" mass="90097" checksum="64AE8FFA37695C9C" modified="2013-10-16" version="1">KLYKNLISATIQAVTSQHGSQTFVTNLGQVLQRQTPGSTLVYTQVSPNSQAQGNYTQTIGTTTGSSRLVTAVNTTVGSNRQIRPLQMNKFSKVGLPASVSIRTPTGPVLSAPVGTTGAVIARNTSGNTVTAGGASLNLTGSTTRIIQLQQPSAGGITTTQIQRAMYQPIIMNPTGAKLNIRPSTKNQQPSLTITQLNKIVPQGTAATSSTSGPLQGQQTITNLSQSAILAPSSTGGAQIISSSGTVVSASTAGGTVIPLLTSRAASLTSIKAPLTVTATLSSTAPPTGXXXXXXXXXXXXXXXXXXXXXXPTGTTINKVTVASGQQQEFNPSTSVFIHTRPSSGSSASTPTLLPPGSTIYYDTISSGNVSTGVLSLNTTTVTSVTSSQIVSTSTPGTYTVIPGGATASVPSSRSFIQIPSTVMTTANTVPPIRYEEKQIAIAMASMNSGTVTTTTTTVTQASSTAASRTVVSVPVSNTAPSNTNILHSVNSAFLRKRDVDGSPVRAAKNLAPTLMSMSSAAPSNMTGITPNAIEQKLATSQPRPGTPPSRPPSTDGSTTVSANSSPGIEQLNEDLIPINRIPNDSHFNPINELYSNHQNTASSVSMSSSSTVPVATPVASTSVAPIVTTTNGCDEPNPPVKKPRRETNDSQKPIVQSAVQQSNQEMKLEEPDEVDGQNDSQIPSTNNKENAKPSSDFVLRNRKNCSLLTTYKNTWKPANNHFIRHSDVKPREDRRPTVMDLANQSHIIKKIEGWKIRRITTQMEELCGLEKQVFDKLKLFLDELEGQENNSDIDRINELVKGNMQRSKIIVDGVNEARNQMLRIFDHQSHVTDIIVRCASKRNFKKREKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX01</accession>
    <name>T1GX01_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX01"/>
    <dbReference type="STRING" id="36166.T1GX01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008338-RA">
        <property type="protein sequence ID" value="MESCA008338-PA"/>
        <property type="gene ID" value="MESCA008338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="OMA" id="NAECAQE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF148">
        <property type="entry name" value="IP09309P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="21020" checksum="32D66B36E8D9F13B" modified="2013-10-16" version="1">MDGIVMFEIHLGARTLNGYNEKGRLILRSNKFIKHEHFNARSAANDIALVKLPQRVEFSKSIQPATLPYQFKNNNLEGYKVIASGWGSENDYSSDMQYTDLKVIDNQECVKEYNSNIIKSSVLCAKGVHHETVCTGDSGGPLALKGGNVVVGVTSFGPADGCLMNVAGGFSRVTHYLDWIRDNTGIQPPVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ8</accession>
    <name>T1GXJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">trypsin</fullName>
            <ecNumber evidence="12">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="12"/>
    <dbReference type="EMBL" id="CAQQ02186536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GXJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008550-RA">
        <property type="protein sequence ID" value="MESCA008550-PA"/>
        <property type="gene ID" value="MESCA008550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="OMA" id="CTEAYGS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF97">
        <property type="entry name" value="GH13245P2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0222">Digestion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="36"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="285" mass="30694" checksum="4FA3494641EEB5A0" modified="2013-10-16" version="1">MNSIIAILTVVAVTGTIIPYIAGDLFPKPRLIDGRIVGGEIVDIVQVPYQISLLYNGFHICGGSLISKTKVLSAAHCVHKQSASMFSIRTGSSRMGFGGKVVKVTNITAHPLYNPRNVDYDFSILDLEDYDEKHVKQSFVSLPGAGDELEXHILCTILSFVSLPGAGDELEDGTMLMVSGWGNTKNSSESNSLLRAVKVPKFNQEKCNRAYQIYGGVTERMICAGFDKGGKDACQGDSGGPLVDAEHKTLVGVVSWGYGCAVPNYPGIYSKVSSVRDWIKENSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXP7</accession>
    <name>T1GXP7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379648">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008600-RA">
        <property type="protein sequence ID" value="MESCA008600-PA"/>
        <property type="gene ID" value="MESCA008600"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624800_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8547" checksum="73634BD45C98E9B2" modified="2013-10-16" version="1">MVGSRATATVTGPPLGPMTSEKFHLKWDSHLPHLNNATPATKLIDVLEPSVYRDIWAVELFYQTGIDTSKSHVNSVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXW1</accession>
    <name>T1GXW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXW1"/>
    <dbReference type="STRING" id="36166.T1GXW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008672-RA">
        <property type="protein sequence ID" value="MESCA008672-PA"/>
        <property type="gene ID" value="MESCA008672"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880604_0_0_1"/>
    <dbReference type="OMA" id="GRINLAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000915">
        <property type="term" value="P:actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01263">
        <property type="entry name" value="PH_anillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000111">
        <property type="entry name" value="anillin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051364">
        <property type="entry name" value="Cytokinesis/Rho-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037840">
        <property type="entry name" value="PH_Anillin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538:SF23">
        <property type="entry name" value="ANILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538">
        <property type="entry name" value="ANILLIN/RHOTEKIN RTKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="22"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008672-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15562" checksum="BBE3590AB12935B2" modified="2013-10-16" version="1">MSPISPLDGTVNMKLDCELEIFVEYQGFLTMFEEISGFGAWHRRWCRLNGHILNYWKYPDDEKKRAPIGSIDLSSCSSTIIQTAPRDICARLNTILVECSRPHQDSDMESLVVVPRGRITIVRSLLSADTKEERDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY70</accession>
    <name>T1GY70_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Cytidine deaminase</fullName>
            <ecNumber evidence="4 13">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 13">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 13">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2'-deoxycytidine + H2O + H(+) = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="4 13"/>
    <dbReference type="EMBL" id="CAQQ02388194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY70"/>
    <dbReference type="STRING" id="36166.T1GY70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008792-RA">
        <property type="protein sequence ID" value="MESCA008792-PA"/>
        <property type="gene ID" value="MESCA008792"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097262_1_2_1"/>
    <dbReference type="OMA" id="LTHFTCV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055086">
        <property type="term" value="P:nucleobase-containing small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000008">
        <property type="entry name" value="Cytidine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004064">
        <property type="entry name" value="PRK05578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="63"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008792-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="16818" checksum="072847B4E048C11E" modified="2013-10-16" version="1">MSLFINGFKDSKNVKEFKDLDSAAQELLLAAIKARENAYCPYSKFPVGAAILTENNEVFTGCNIENGAYTAGLCAERTAAAKAISEGQIKFKAIAVVALQENGFTTPCGVCRQFISEFALNGDVPIYLAKPTNPPLRVFCTSIYQLLPESFSFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYJ6</accession>
    <name>T1GYJ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYJ6"/>
    <dbReference type="STRING" id="36166.T1GYJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008925-RA">
        <property type="protein sequence ID" value="MESCA008925-PA"/>
        <property type="gene ID" value="MESCA008925"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1273519_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008925-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24646" checksum="6833DF1B41C21881" modified="2013-10-16" version="1">MLKIFDDSMNNLETAAGEGDGYETDSESDTDSVSSAESASSVRILIESMPPKHSDKIVFDDSSDSEEEGERIISAINKSPNLIPNPETKNSEIFVFRPYLQPFQERRRLSQCKEEEEEDSLSSPQPSSQPLPTPPPPVALQDSPMDVGKFRELEKLRQQFMEKLENNSLKLDTPKTRKEKKSQPLKKCLEAKEETKDKELNSKVLISIKQFLRIKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYS6</accession>
    <name>T1GYS6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374506">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009013-RA">
        <property type="protein sequence ID" value="MESCA009013-PA"/>
        <property type="gene ID" value="MESCA009013"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111626_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009013-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="13371" checksum="FC9FC83A3D75A9E0" modified="2013-10-16" version="1">MNGNSLYKEKIDGFLNIIRDVGLYQHWTTGAFTDALKYNQRDFIELCPHYRNINVYNFTKKIKFDDIPRIQALNVDFFSYLLLEFAIGLIPPLADFSMSDGNSRFVARFVPASYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU9</accession>
    <name>T1GYU9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009036-RA">
        <property type="protein sequence ID" value="MESCA009036-PA"/>
        <property type="gene ID" value="MESCA009036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712017_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8258" checksum="9A8DEB6A407EB241" modified="2013-10-16" version="1">MISRRLQKTTTIFLIGSFKSLVKWENGDNIIYNKSDQILAYAIDFDVIGTLYEVIDKFLAIEKASNSVGLNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ24</accession>
    <name>T1GZ24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009115-RA">
        <property type="protein sequence ID" value="MESCA009115-PA"/>
        <property type="gene ID" value="MESCA009115"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657319_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009115-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8794" checksum="B9A5E5C3E5C74123" modified="2013-10-16" version="1">MFSCTVNKQIERLAKFQKSLIDVARTSLTTKLHPKLADILTEVSFGYATQNNHRLLYCYGYGYGSWRTSFRHTKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ35</accession>
    <name>T1GZ35_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ35"/>
    <dbReference type="STRING" id="36166.T1GZ35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009127-RA">
        <property type="protein sequence ID" value="MESCA009127-PA"/>
        <property type="gene ID" value="MESCA009127"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796901_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004530">
        <property type="term" value="F:deoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF37">
        <property type="entry name" value="ENDORIBONUCLEASE DICER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009127-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7686" checksum="60BE651368C6B33D" modified="2013-10-16" version="1">MDNAAAKKGSYLLQAMTHASYSPNCLNACYHRLEFLEDTVLDYAISRYLYEHKRGHSPGALTDLSFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ38</accession>
    <name>T1GZ38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macroglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ38"/>
    <dbReference type="STRING" id="36166.T1GZ38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009130-RA">
        <property type="protein sequence ID" value="MESCA009130-PA"/>
        <property type="gene ID" value="MESCA009130"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_950898_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF172">
        <property type="entry name" value="LD23292P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macroglobulin" evidence="1">
        <location>
            <begin position="160"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009130-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="293" mass="33240" checksum="713ABC55D96D3F20" modified="2013-10-16" version="1">MVELSAFERWWMGFNSRYFCSFAPPPLGLSSSFDNYGGGKSSYNIKTSFLETVFSKEPTYFIVASRMVRPGLIYQVAVNVLHAAYPITVHASISKDGVEISGDFQNVKEGVPETLLMRIPPTSVYGKYKLRVEGLYNHVLGGIAFLNETDLVFSQRSMTIFIQTDKPLYKQGEMVKFRAIPITTELKGFDNAIDVYMLDPTGHILRRWLSRQSNLGSRNVKWFLQHQSDDGAFYEVTWLPDRKMNKTGFTDEFGEVRQKTISLTAHVLITLATVNDLSGNKSIFGSKTRFTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H011</accession>
    <name>T1H011_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H011"/>
    <dbReference type="STRING" id="36166.T1H011"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009475-RA">
        <property type="protein sequence ID" value="MESCA009475-PA"/>
        <property type="gene ID" value="MESCA009475"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020515_9_0_1"/>
    <dbReference type="OMA" id="AGECMIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13751">
        <property type="entry name" value="TGF_beta_GDF8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF262">
        <property type="entry name" value="LD29161P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="7">
        <location>
            <begin position="15"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009475-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14091" checksum="160FD76C5994A5B3" modified="2013-10-16" version="1">SLYLEVKAKPIGKKRRKRQDSLDCNENSAIAECCRYPLLVNFAQLNWDWIIAPQTFEASYCNGECRMGFLPESVHTTISHQVGTQAKPCCSPSKLSAVDLLYFSQDLQILKTRIPNMAVEKCSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H032</accession>
    <name>T1H032_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02148275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H032"/>
    <dbReference type="STRING" id="36166.T1H032"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009498-RA">
        <property type="protein sequence ID" value="MESCA009498-PA"/>
        <property type="gene ID" value="MESCA009498"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034585_2_2_1"/>
    <dbReference type="OMA" id="AVRNCRW"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17047" checksum="FDD81496471CF213" modified="2013-10-16" version="1">MVHFGHANSLRQAKQLGDKLIVGVHTDEEIAKHKGPPVFTQEERYKMVRGIKWVDEVVENAPYVTTLETLDKYNCDFCVHGDDITMTADGKDTYQFVKDANRYKEVSRTAGVSTTDLVGRMLLLTRNHFQQGKHEFTVNKEGMFLKEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H047</accession>
    <name>T1H047_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H047"/>
    <dbReference type="STRING" id="36166.T1H047"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009515-RA">
        <property type="protein sequence ID" value="MESCA009515-PA"/>
        <property type="gene ID" value="MESCA009515"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_964963_0_0_1"/>
    <dbReference type="OMA" id="YPRSYIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009515-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="289" mass="34745" checksum="23920455D1EBF235" modified="2013-10-16" version="1">TYWRQPRYNRDQHEEYPQRRQSGNFEDDRQSVRSEGGTLKIYNVKDKQRHNNHNRNSNNNWNNSHRYNQGRRYENDSGSRSGSNYNLDYVGNNNRSNENLNEKRSNNYRNNNTYERPPRFRQKDMSKTWRHNNSRPSSSTRDEYRDDSRDQFNNSRSSSRTRTLPRNSRRGGNMNNGRRSVSPSHSTVSERNFRPHQQRHFGSNNHQNNNNTRYSSQSSLASDYGTWDRRSNNFRRNDHGPNNKFRQHDSWNRRNDSHNNDHESISNTNDEIVKHARETTKYLETLSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0W2</accession>
    <name>T1H0W2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02386782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0W2"/>
    <dbReference type="STRING" id="36166.T1H0W2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009807-RA">
        <property type="protein sequence ID" value="MESCA009807-PA"/>
        <property type="gene ID" value="MESCA009807"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_24_2_1"/>
    <dbReference type="OMA" id="FRCKIAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF1">
        <property type="entry name" value="RE17845P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009807-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14859" checksum="E2854543508770C4" modified="2013-10-16" version="1">MTNMRPPNKTILLKVVILGDGGVGKSCLMNRFVSNRYDENSFHTIGVEFMNKDLEVEGEKYTLQIWDTAGQERFRALRTPFYRGSDICLLCFALNDSSSFRTLGQWKDEFLKYADIKSEQFPFIVVGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1S0</accession>
    <name>T1H1S0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385554">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1S0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010138-RA">
        <property type="protein sequence ID" value="MESCA010138-PA"/>
        <property type="gene ID" value="MESCA010138"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1572422_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010138-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19348" checksum="000C601369C39AC8" modified="2013-10-16" version="1">MHTTPNINADSNLTSADVRVQLSDPHSSWRYSLTFVLRDNCLDHSSLIRIRFRICNKEEKTPSTITEVGKAIERLKNYKNTGTDDIRLQYPRESHQTMTLRNTWSCVRAANGISLILQTMLGFRQGYALSCSIFYNLLETIVRAAKIKIFSKSTGVRTAFQGLSIESKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1X9</accession>
    <name>T1H1X9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1X9"/>
    <dbReference type="STRING" id="36166.T1H1X9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010203-RA">
        <property type="protein sequence ID" value="MESCA010203-PA"/>
        <property type="gene ID" value="MESCA010203"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029293_0_0_1"/>
    <dbReference type="OMA" id="QLPHKFE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="433" mass="48855" checksum="AE308313E020A050" modified="2013-10-16" version="1">MVSLPNINRPTSDQIEDGKIEYDNATGATIEYKLIKGGYEVKTTTPQPNGTKKMQVQTFWDPMPADELKRVIAEHNASNKKPNGTIERIDKNTTVEYRDIEGGHEEITTTIMEDGTKKEDFLRAKISDDSEPAPKHIQQQQQNKNKAVQQKKDVQNKSLVETQNRKATLKKTDSTDSQSSQSHFEQKTVEDYRKVTQTSVKTSHRKNSVQSVETVSSRKNSLVEEYQAIEAPKEKPKKKKTKGVPAPPSGFIENDSTVVTSKKVNGGTEYNYTTTLEDGRTVTTTKIVYEEEEVEMTEEEIEQYKKELKDAEKHKDITKAIKLKSPSGTKKVIPSENPGEVTTVETIKFEGYTEFHYSTVCSDGVVKHSTKTVYDAVQSVEDDEEEIVEEIEEEIIEPGETIVKTFESTKLVEKNYAETTVTKKGKKTKKIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Z5</accession>
    <name>T1H1Z5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Z5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010220-RA">
        <property type="protein sequence ID" value="MESCA010220-PA"/>
        <property type="gene ID" value="MESCA010220"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010220-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7411" checksum="CDA0E9EE59118A33" modified="2013-10-16" version="1">RERGIFPGNFASHYSYILKEDIIATLIDNNIPRSNDSMNLCDYKQIKISKNQPKSAWEVRLIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H267</accession>
    <name>T1H267_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the activator 1 small subunits family. CTF18 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H267"/>
    <dbReference type="STRING" id="36166.T1H267"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010300-RA">
        <property type="protein sequence ID" value="MESCA010300-PA"/>
        <property type="gene ID" value="MESCA010300"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004894_4_0_1"/>
    <dbReference type="OMA" id="HFFISIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001083">
        <property type="entry name" value="Chromosome transmission fidelity factor 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053016">
        <property type="entry name" value="CTF18-RFC_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46765">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46765:SF1">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="116"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="493" mass="55924" checksum="4DD0468F7E2A796B" modified="2013-10-16" version="1">MTALEAETEPDVVEIPSAGLASDEGNLWVDKYKPRKYTDLLSDESTNRTLLYWVKMWDKVVFGKDFRKPKTAYEKQQQNLNSFNKRTGRFEQTGGWKNRKSRLNLNTEYDEHDRPIQKVALLSGPPGLGKTTLAHTIARHAGYKIRELNASDDRNPEAFKLTLENGTQMSSVLDLEKRPNCIILDEIDGAPQASIDFLMRFINDSAQNKPAKKGVKEANSILKRPIICICNDLYAPALRQLRQVAFVVNFPPIETAKLAERLLEIANQEGLQTDLMALMALAKTAKLAERLLEIANQEGLQTDLMALMALADKTGNDVRSCISLMQFFNATKSKLVLTDVLNSNFGTKDRNAGLFEVWGSIFQIKRPQKRLETNNNNEEAQVVMTNMSAATRMNNVLAVVHSGGDYERLMQGVYENFLHQKMPDPNLTGVTEAIQWFCFSDILNQQINHLQNYTLYGYLQFPFVIWHLLFASLAWPKINFPHQGVEALEKIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X9</accession>
    <name>T1H2X9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2X9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010585-RA">
        <property type="protein sequence ID" value="MESCA010585-PA"/>
        <property type="gene ID" value="MESCA010585"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778743_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010585-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7736" checksum="8A29EE3FEE96A4CB" modified="2013-10-16" version="1">MNLEVESLLAITQLILVRADVRLKQELRNTESGVFSKSDIDLVQLPDGFTGGGQRRALYDPFAIKVEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3F9</accession>
    <name>T1H3F9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3F9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010785-RA">
        <property type="protein sequence ID" value="MESCA010785-PA"/>
        <property type="gene ID" value="MESCA010785"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742957_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577342" evidence="1">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010785-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8027" checksum="11A1B8FAEBD62C74" modified="2013-10-16" version="1">MNFKVMQILVALFAVLFSQSSACTKNKEFCTLDSQCCSNYCHYDYENSQPNACTPPPEGLCFNGSIWLRCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3U3</accession>
    <name>T1H3U3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02375206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3U3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010932-RA">
        <property type="protein sequence ID" value="MESCA010932-PA"/>
        <property type="gene ID" value="MESCA010932"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624796_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010932-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9372" checksum="68CBCF73E3525BDE" modified="2013-10-16" version="1">MRKATYEFLGQQERKRPKEWRDAEYDMAADMDTACNLRNYKCNLYVNYRSIRFRLINSCIPTIHGFSVLFASWGRQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H495</accession>
    <name>T1H495_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02009763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H495"/>
    <dbReference type="STRING" id="36166.T1H495"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011101-RA">
        <property type="protein sequence ID" value="MESCA011101-PA"/>
        <property type="gene ID" value="MESCA011101"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_935602_0_0_1"/>
    <dbReference type="OMA" id="REMEINH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="5">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="174"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011101-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="298" mass="34097" checksum="909B6821BACBFBF7" modified="2013-10-16" version="1">TYDEITIYPQPNLNVITGPNGSGKSTIVAAILLVMGGNPKLLSRASKIEDYIKNGKEQADISIVLYIDKTSKTTFRRMFNKNGKSEWMINNQATSLKNYQKKVSEFNIQIDNLCQFLPQDRVQDFTSQNPQELLLSTQTSVCDLEVVGWFETLKELRSVQVSAKKRSGDNAKKLAEATKRNAQLQEIINRINERNEFIEEIKVREAKKAWFEVKELETKLQTTKGELQNAESFIKRDKKALNEIEKQAKNVINDAEKFKKIINNGQNRLESLKADMEKINASNENFEKELRCAAKDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4T4</accession>
    <name>T1H4T4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4T4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011302-RA">
        <property type="protein sequence ID" value="MESCA011302-PA"/>
        <property type="gene ID" value="MESCA011302"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3421488_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011302-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="24" mass="2588" checksum="6BD2F28899F1AE3C" modified="2013-10-16" version="1">MTLLLGYIGNEDIDLFSSSSSVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4X9</accession>
    <name>T1H4X9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4X9"/>
    <dbReference type="STRING" id="36166.T1H4X9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011350-RA">
        <property type="protein sequence ID" value="MESCA011350-PA"/>
        <property type="gene ID" value="MESCA011350"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745143_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011350-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="16732" checksum="E2CAAF071E7438A6" modified="2013-10-16" version="1">IHQGLGKHITQDLEPALQFCTQLACQLQENIKFERNSPHLKVLLGVGGDVNITPEEETNNSLKLFGLTIPREQAKEDSQCFCTNFKKQFSGDFVVDEKADEIKEQFTVTGHTPIVYTKVVSEGGLQSITPGLFNPSNIYLKDANDPLRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H557</accession>
    <name>T1H557_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H557"/>
    <dbReference type="STRING" id="36166.T1H557"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011432-RA">
        <property type="protein sequence ID" value="MESCA011432-PA"/>
        <property type="gene ID" value="MESCA011432"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3194083_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011432-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="46" mass="5053" checksum="123F5CC063D7EC60" modified="2013-10-16" version="1">MIGLSCPVTYNPADFIIEVTAGEYGPDHLDQMVNAVENGKVTRWCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5T9</accession>
    <name>T1H5T9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase MARCHF5</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Membrane-associated RING finger protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Membrane-associated RING-CH protein V</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">RING-type E3 ubiquitin transferase MARCHF5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="STRING" id="36166.T1H5T9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012072-RA">
        <property type="protein sequence ID" value="MESCA012072-PA"/>
        <property type="gene ID" value="MESCA012072"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046472_1_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16701">
        <property type="entry name" value="RING_CH-C4HC3_MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000262">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46283">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="21">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012072-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="31822" checksum="B2E7FC71BA007162" modified="2013-10-16" version="1">ENDEEERTCWICFATDEDNKLAEWLQPCKCRGTTKWVHKSCLNRWVDEKQKGNAFKTVVCQQCQTEYIIVFPKLSKFANVLETFDHLCRRLSPYFAAGVIVGSMYWTAVTYGAVTVLQVVGHKEGLALMENGDPLLLLIGLPAIPVGLVLGRMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQENNQQPPQQDIPTPAMSDPSLATRAFCGALLLPTISTIVGKIFFDSIESPLHRTLLGGLTFIVVKGVLKIYLKQQQYNRKKERQIVDYTDDNIRMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H610</accession>
    <name>T1H610_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acylphosphatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H610"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012143-RA">
        <property type="protein sequence ID" value="MESCA012143-PA"/>
        <property type="gene ID" value="MESCA012143"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3112418_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="51" mass="5665" checksum="080D9B52D781D45D" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXHTQRQANSLGLRGWCMNTPNGTVKGRLEGPEKPIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H677</accession>
    <name>T1H677_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H677"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012210-RA">
        <property type="protein sequence ID" value="MESCA012210-PA"/>
        <property type="gene ID" value="MESCA012210"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140672_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15592" checksum="4F8110520F42806E" modified="2025-10-08" version="2">MKVEKGIPNFELFGTKHLIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIQTRHTKFQRELVREVCGHAPYEKRVMELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNILTQMRKAGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6A9</accession>
    <name>T1H6A9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H6A9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012242-RA">
        <property type="protein sequence ID" value="MESCA012242-PA"/>
        <property type="gene ID" value="MESCA012242"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_537806_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="476"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="489"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012242-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="56949" checksum="1BB650F65FE782CB" modified="2013-10-16" version="1">MATILDYMIFVLLLMSFVINIIAIGAFRVSPGLQTMANRFVINLLIVNLIGCLVLVPTLFLNVSMYENKEVDESSIAIKEIGLRELSIKENIEXCNHQKEKLIAQFLVVARHIQNEDNEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRAARISSLVIIFYLITYIPYGLLVLFQVSLDFMYAKQLTIFLVTLAYISGPFIFAYRNKRVRRHSETVPGKKPKXKDNNNNDSNNYSGSNNGNNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6J0</accession>
    <name>T1H6J0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain Tctex-type family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6J0"/>
    <dbReference type="STRING" id="36166.T1H6J0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012323-RA">
        <property type="protein sequence ID" value="MESCA012323-PA"/>
        <property type="gene ID" value="MESCA012323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097204_4_2_1"/>
    <dbReference type="OMA" id="RTIMNRN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21451">
        <property type="entry name" value="DLC-like_TCTEX1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF69">
        <property type="entry name" value="AT23443P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="13598" checksum="503EC26146CA3529" modified="2013-10-16" version="1">PVLRYAPTYRLESKKPFNREKAENVIKKVLEENLEDFVYGPKHATNLCKHISEEIKIRIKYKYVCFVTIGEKFLQDNCMMNFLWDSNNDGFLTHLHETPEYFVLVTFIYTYYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Z0</accession>
    <name>T1H6Z0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Beta-1,2-N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">GlcNAc-T II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Mannoside acetylglucosaminyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12941"/>
            <dbReference type="Rhea" id="RHEA-COMP:13526"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60651"/>
            <dbReference type="EC" id="2.4.1.143"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 16 (GT16) protein family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.143" evidence="5"/>
    <dbReference type="STRING" id="36166.T1H6Z0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012472-RA">
        <property type="protein sequence ID" value="MESCA012472-PA"/>
        <property type="gene ID" value="MESCA012472"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375277_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008455">
        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007754">
        <property type="entry name" value="GlcNAc_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871:SF0">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871">
        <property type="entry name" value="BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05060">
        <property type="entry name" value="MGAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="123"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="177"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012472-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="23389" checksum="EB4EE933A6A713D5" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIFYPYSIQTHPDCPRDIKKDQRKCNNALHPDLYGHYREAQFTQTKHHWWWKANRVFDQLEITRYHTGLVLFLEEDHYVAEDFLYLLELMQRRALELCPQCNILSLGTYXRLSTITYNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7B0</accession>
    <name>T1H7B0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7B0"/>
    <dbReference type="STRING" id="36166.T1H7B0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012648-RA">
        <property type="protein sequence ID" value="MESCA012648-PA"/>
        <property type="gene ID" value="MESCA012648"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091463_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097345">
        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF5">
        <property type="entry name" value="BNIP3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="39"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012648-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16670" checksum="CD12A0FFBBDCA3A0" modified="2013-10-16" version="1">SWIELTSTAAMMGMRSPERITPQPFIVEEYLRLLREAQRESNQSSRVVSLASSRRDTPRDSPKSPPNSPNNELHNDDHLKNIYINYWSGRHRLDLSGAQTRSNPPKTRNFQDSMRQADWKSLFSREILYSLILTNVLSLLLGTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA03</accession>
    <name>T1GA03_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196834">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA03"/>
    <dbReference type="STRING" id="36166.T1GA03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000045-RA">
        <property type="protein sequence ID" value="MESCA000045-PA"/>
        <property type="gene ID" value="MESCA000045"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006779_3_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048869">
        <property type="entry name" value="OCRL-1_2_ASH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF300">
        <property type="entry name" value="TYPE II INOSITOL 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21310">
        <property type="entry name" value="OCRL-like_ASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000045-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="535" mass="62136" checksum="999766AA7DA5BB1C" modified="2013-10-16" version="1">MLSIFVKQDIRQQVTRYTVSTAATGVFNVMGNKGGVAVSLTLNMTDICFVCSHLAAHMDQIERRNLDYAEIMKRCTFDEDFRKRTIREHDQIFWLGDLNYRINEIPGQYVDRSDLAGLLRNDQLTQEMRKNNVFQGFTEGIIGFNPTYKYDPGTDHYDSSEKQRAPAWCDRILRKGDRIKLLHYDSVMEIRQSDHKPVYAVFSSGIKTKDDKKYKRVHEDVLKTVDKYENDNQPQISVEKTEIDFNEIRFNEKFSRDFTIANSHHLSANFSFKAKEENQICKKWFKVEPTSGTLLTGDSLIIRITFKGLDSRNIGEFLKLVKCGGSVPLDILVLHVQDGRDIFITINGEYIPSCFGLDMDLLCRTERPIMEYSLQELAEIEKSNCDYKVTMPREVFFLIDYLSASVENVKDVFHLDRKFCKHVSLNLVRDWLDSWSRHDFQAILVTESFTESSGYILKLSAPKRNVFLHLCMFLKEIESKGNNINDLAMLFGNVLVKGRRQVPGYRDGRCQQFMLRFLRNKDVANFALENVQMEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAK0</accession>
    <name>T1GAK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000258-RA">
        <property type="protein sequence ID" value="MESCA000258-PA"/>
        <property type="gene ID" value="MESCA000258"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471640_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000258-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10038" checksum="F4AF40BD3EAA33FC" modified="2013-10-16" version="1">MHLISVGNERILLSIYLTKNLCIAFETSKPNAKIRNQLQTEIEEHDLIAKDISPPHDHESTLKTAYKHTKAKLLSFSQRMHSPQKDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAS7</accession>
    <name>T1GAS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAS7"/>
    <dbReference type="STRING" id="36166.T1GAS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000338-RA">
        <property type="protein sequence ID" value="MESCA000338-PA"/>
        <property type="gene ID" value="MESCA000338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072226_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="4">
        <location>
            <begin position="62"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="19078" checksum="DBA0DD9C3B2957E9" modified="2013-10-16" version="1">MMGPLSKLALFRPTICAVSHMSVYPKNYIEPIFKKEDQEALTKSGEHLKVAHIPIKAARNNDTVSVFHDEVISKFTNYIMKKGNKLLARDLLSKTFEQIKRIQLEKYNLSQNEEEKSKIQTNPNEILHQAIENCRPLLQLTPIKRGGVTYRFLFQSPLTEPILWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB41</accession>
    <name>T1GB41_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB41"/>
    <dbReference type="STRING" id="36166.T1GB41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000462-RA">
        <property type="protein sequence ID" value="MESCA000462-PA"/>
        <property type="gene ID" value="MESCA000462"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1385619_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="21547" checksum="F2243C610F6FBE0C" modified="2013-10-16" version="1">MPPVNLVNQRISAFGGGKNQNQAHQPQSPRTDYQGNYNDAGVQKTFNTQRNVYTTVNNGNTITTTITTNTARSQWHYSTSDTSPPGPAVTNPAYMGMGFTQNQNQSDNLTGWGSSRFETGRPVSLQPDMASNLSRVSAESSSESEAQSISSLSGIKSGTGKNREDRKSGMFRLFQRNKHSEKDKSSANSSRRSSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB47</accession>
    <name>T1GB47_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02079115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000468-RA">
        <property type="protein sequence ID" value="MESCA000468-PA"/>
        <property type="gene ID" value="MESCA000468"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107288_3_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857:SF43">
        <property type="entry name" value="GEO07291P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857">
        <property type="entry name" value="ODORANT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00708">
        <property type="entry name" value="PhBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577227" evidence="5">
        <location>
            <begin position="17"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000468-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="14123" checksum="A22E96D0D97A0BF2" modified="2013-10-16" version="1">MKAFLVFAVILAVAVAEMHEMGEQFKGECMDQEKVDMSELKKVQLDDPTEKLKCFIRCLFEKGGFFKDGIFVDEKVKEFLNKQAQKEHMLAAYDDCKNIKGTNDCDTTFKMFLCMERAQFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBN2</accession>
    <name>T1GBN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02083912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBN2"/>
    <dbReference type="STRING" id="36166.T1GBN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000668-RA">
        <property type="protein sequence ID" value="MESCA000668-PA"/>
        <property type="gene ID" value="MESCA000668"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_426609_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046987">
        <property type="entry name" value="Myo9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184:SF5">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="204"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="266"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000668-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="73256" checksum="F9C5D4C1ACEC59BF" modified="2013-10-16" version="1">MNLNRQNYQTGKTKIYMRESEKLLLDTKLHRKIIGSIITIQRWFRGILERNKFLMYKTSAVTLQSYWRMYLTRKKLQVERIHVNAAIIIQSAWRMYKQKTWYKKLKKGVVMFQAHVRGYVTREKIKNHKQKVQRERNQLKSTQSLPVVNRSADILKSKEDSLSNSSKKIFHQQPHFSLDSKYSSTSIYEGSEKLYSNIKPQTASSSGNLSRLSSSGSSSVVIDNTTAPYYYSSSSSSPTNIHDATHQTHESLRNLMISSPTSATASASVASSSAPSSSSGAPTTPTSTILSSLSNINNSSNKMHLYQQSHKDSNGSSSRNSSSYSYDSENSSLLTNTSSSSPIKRQATQSLQQQQQQFLQSEINKAPIRRTDSNSGIQSSSQHVVDAAATALHPKNQFHPIMKSMSDSVSPSDIIAGHHYQPHGKYVKRIGSGANEIDEEVNDVFEISKLLNNYARYDEVLRMKFFVLGHDMHVRLSESNDFNSMHYLIYFHGSGGGDTYKTALARTDNLYNSWYVLEEAYETVHTDRFYYTMLHIIVKKDGLLTFNMNGTDHLLSSRVYDDFPPYYIAFSKSSYEESSRIFFDCPEDVTTTEADYLVESTERHANYLEEYTYRNVFYVSKASRTSYKFIFLSAIVYLYLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC32</accession>
    <name>T1GC32_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000828-RA">
        <property type="protein sequence ID" value="MESCA000828-PA"/>
        <property type="gene ID" value="MESCA000828"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_752923_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="2">
        <location>
            <begin position="246"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="368" mass="43365" checksum="B17459A1DC04C8F7" modified="2013-10-16" version="1">MDDLEIQIAPDSNLFAVIRTVEKGKFNYSTVLMKWLYSTEDGRYLVKWPQHYERAIKEKASPTNIWQTFLCSIKARNIDSKLAAKNVEKTYYEDGSTDSQQEKQNRKILKRRHPVTTLGFDENSNYNESQVIYPNINEHGSTEEPHYEKENNESNLEPVPHLDNSQNENVYSDYNQVLESNFKDFRIAFRTFIWFYFQKSNDANIQSILKMCKTIVKVTTQLHIDMEKLSKKIESFSVPNIENCETVFKFPLKKKKQINEFEVSLENEETFKRFVSWIKQIGGFDIDHYLLNVARNLFKDQVLKKYSLCGRKEKICFKNFKNILNAHVMGISQSLKTNMTLQDVHSFYGRYLKNAGSRLSQKDRKNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCG4</accession>
    <name>T1GCG4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02168134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCG4"/>
    <dbReference type="STRING" id="36166.T1GCG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000976-RA">
        <property type="protein sequence ID" value="MESCA000976-PA"/>
        <property type="gene ID" value="MESCA000976"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2114987_0_0_1"/>
    <dbReference type="OMA" id="WKTGGCP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000394">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056754">
        <property type="entry name" value="DSCAM/DSCAML_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25059">
        <property type="entry name" value="FN3_DSCAM-DSCAML_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000976-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="12630" checksum="330819F3E867867F" modified="2013-10-16" version="1">IGAGEASDILNTRTKGSKPKQPAKSSFIEVSSTSVSLHFKAWKDGGCPCLTLDQLEWNQISNNVKPDNNYVVLDLEPATWYRLRITAHNSAGFTVEEYDFATLTVTGGMFLISDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCG8</accession>
    <name>T1GCG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Reticulocalbin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum. Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3. By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis. Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens. This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PCSK6 (immature form including the propeptide); probably involved in the maturation and the secretion of PCSK6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02043142">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCG8"/>
    <dbReference type="STRING" id="36166.T1GCG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000982-RA">
        <property type="protein sequence ID" value="MESCA000982-PA"/>
        <property type="gene ID" value="MESCA000982"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044718_0_0_1"/>
    <dbReference type="OMA" id="TWEEYNM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16227">
        <property type="entry name" value="EFh_CREC_RCN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000104">
        <property type="entry name" value="calumenin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF95">
        <property type="entry name" value="LD34388P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577261" description="Reticulocalbin-3" evidence="12">
        <location>
            <begin position="21"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000982-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="335" mass="39108" checksum="75B5DF113998FEA4" modified="2013-10-16" version="1">MMKYSILILFTISILEAIAAVPPHKHEKHSSGSKERIKDGIYQGRDAKHYEDGAHHSEFDHEAIIGSVKEAEEFDSLTPEESKKRLAVLVKLMDLNHDGFVDRHELKAWILRSFKKLAEEEAEDHIDEIDTDGDGRVSWKEYLVDNYSMEDENEKKELIEFETYEEEQKMIKDDKEMFKAADSNQDGLLDLQEYVLFQNPEEHPQMLPIVYEQTMRDKDKNQDRVIDFKEFVGEEASRHDKEWLISEKDKFDNDFDLNRDGVLTGNEVLSWVVPSNDEVAKDVADHLFVAADEDHDDRLSYIEILNNHETFVGSEATDYGDHLENIDRFTDELFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCY1</accession>
    <name>T1GCY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Golgi phosphoprotein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02084947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCY1"/>
    <dbReference type="STRING" id="36166.T1GCY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001154-RA">
        <property type="protein sequence ID" value="MESCA001154-PA"/>
        <property type="gene ID" value="MESCA001154"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036311_0_0_1"/>
    <dbReference type="OMA" id="CWIRKWA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031985">
        <property type="term" value="C:Golgi cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001154-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21888" checksum="37DA8B4CB6953B0A" modified="2013-10-16" version="1">MRRRGLCSRKLILKSDQPTGDVLLDEALKHIKETDPPETVQSWIEYLSGETWNPLKLRYQLKNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLSDNVVKCRLVKMIQDSVISKWVNDTQRMEKRVLALIFLAHASDVLENAFAPLNDDDYEVAMKRVRELLDLDFEAEAAKPNANEVLWAVFMAFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDC9</accession>
    <name>T1GDC9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDC9"/>
    <dbReference type="STRING" id="36166.T1GDC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001313-RA">
        <property type="protein sequence ID" value="MESCA001313-PA"/>
        <property type="gene ID" value="MESCA001313"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123907_0_0_1"/>
    <dbReference type="OMA" id="ATRFIMY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF14">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN KINASE DAPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001313-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12931" checksum="190260D5160E54A0" modified="2013-10-16" version="1">MASVRSVQVKLIDFDSVQKVSKLGTKVPVSGILDFQAPEMVNDEVVFPQTDIWSVGVLTYLLLSGCSAFRGNDDIETKQNISCVRYRFENLYKEVTAEATRFIMYIFKRSPQDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDJ2</accession>
    <name>T1GDJ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDJ2"/>
    <dbReference type="STRING" id="36166.T1GDJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001380-RA">
        <property type="protein sequence ID" value="MESCA001380-PA"/>
        <property type="gene ID" value="MESCA001380"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_9_2_1"/>
    <dbReference type="OMA" id="ILLWMTT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
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        <location>
            <begin position="140"/>
            <end position="170"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="235"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="321"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="425"/>
            <end position="454"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="504"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001380-PA"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="60909" checksum="8369ED1DF9D33271" modified="2013-10-16" version="1">MDGAVSNNNTIQLSFFERLSLLFKYHWRGFIVFITPLIFLPVALMGTDAYECLYVLLIMAVYWVSEVLPLPVTSMIPIVAFPLFGILSSERTCQLYFKDTLVMFMGGIMIALTIEHCNLHKRLALKVIQIVGCSPRKLHFGLIVTTTFISIWINNAAATSMMCPIIFAILEELQNDGDEPKSPSKMAIAFYMGVAYASSIGGCGSLIGTGTNLTFKGIYDTRFPNAKDTIEFGTFIVYSLPIVILSSCLMYVYLQWLYMGMFRPKSKEAQDANIGEEGARVAKGVVDQRYKELGPMTSHEKGVLILFILMIVLYFTRNPGFMPGWGNYFGLSNVKNSLPTILIVVLLFILPAHWGFVNFIIGRAPYPSDATPSMVNWKFLQQRVPWGLIFLIGGGFALAEGSKQSGMAAMIANSLNILGDLPKPAILFIVILIAEFLTAFSSNVAIANIIIPVLAEMAVSIGIHPLYLILPAGLACSMAFHLPVSTPPNAIVAGYANVKSGDMAIAGIGPTIASIGVLFLICQLWGVVIYPDLNTFPSWALGNEVINATVKAALNATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDY8</accession>
    <name>T1GDY8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001541-RA">
        <property type="protein sequence ID" value="MESCA001541-PA"/>
        <property type="gene ID" value="MESCA001541"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592497_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001541-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9665" checksum="7181188DB99685B8" modified="2013-10-16" version="1">MALFMEQMNFNKNRKKVYIVIRINSNISKVVLSELKNHLWYLSSDLILLTLYDDRIDCNIKHKILLKKLFTINKHVFRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE74</accession>
    <name>T1GE74_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <taxon>Megaseliini</taxon>
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            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="33"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001632-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16525" checksum="C3EB5CE79FDB0160" modified="2013-10-16" version="1">MIIFPTVPIKSTPSSSQQRNLSKFMENVEGPFAMLRKCRESGNHAKIYIRNNKTIVGHTMGRIIAFDKHMNIILEDATETFKRRKFKYSEDKYVGESKDCSQRLLNLGITLPESKVKSVNRKNCIVSRYVGKVLIRCECIALII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE77</accession>
    <name>T1GE77_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02045984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045988">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE77"/>
    <dbReference type="STRING" id="36166.T1GE77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001635-RA">
        <property type="protein sequence ID" value="MESCA001635-PA"/>
        <property type="gene ID" value="MESCA001635"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010922_0_0_1"/>
    <dbReference type="OMA" id="RYKPYRS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ligand-dependent corepressor isoform 1"/>
        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
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        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF102">
        <property type="entry name" value="PIPSQUEAK, ISOFORM O"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_psq"/>
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
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        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="6">
        <location>
            <begin position="457"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="HTH psq-type" evidence="6">
        <location>
            <begin position="512"/>
            <end position="564"/>
        </location>
    </feature>
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        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
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        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="331"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001635-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="665" mass="72355" checksum="24AE655E303D2C25" modified="2013-10-16" version="1">GLLRTAEQLKIKGLCETAETVDEILPETATTITLPETVVIGGHHHQQQQQHQTQQQIVHTISNAANTNIGTIVATTPTSSRKSRLGKRSKSPESQLQQQQTILATSQTITGPAIVSLHQTSSGNFIPVSVSGQPITTTSALSNSSDSENKVIQAKICKTEGGNNNTSTSGTTSGSSTVSNGSLLGMQMGFLDFAPEPPAPSATPVTVTEHVDMSCTPSNDTRDLSNSTPNNGDVRIKFETLRSLDPTETLDIDNHLAQHIIQRLDQSPMHHQLQQQQQQQQHHQQQQHHQQQHHQQQQHHHGNEDSGGGGHNLIQTIKTEVIEVKHHQQIQHHQLQSQGMNQGQSVQQHQQQQQNQSHQELTGTTVMEIDPSQIKNEPGMIITPEIVTMMTPGMDQQGQHDNKGQLNGPKTWTQDDMNSALDALQKQNMSLTKASVTYNIPSTTLWQRAHRLGIETPKKEGSTKSWNEESLNNALEALRTGQISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLERALDAIRSGSTSVQKASSEYGIPTGTLYGRCKREGIELSRSNPTPWSEDAMNEALASVRVGQMSINQAAIHYNLPYSSLYGRFKRGKYDTGNNSNVGIANTSTGSNSGGAPGANTSSGTIEIIEHSPENSVSVSIMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEE1</accession>
    <name>T1GEE1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02016341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001703-RA">
        <property type="protein sequence ID" value="MESCA001703-PA"/>
        <property type="gene ID" value="MESCA001703"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770186_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577266" evidence="1">
        <location>
            <begin position="19"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001703-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="17313" checksum="98E0A375C6BA34B4" modified="2013-10-16" version="1">MKLIHTLAFIALVLPILATRLDLYHSADSMTMCMKKFDVTWELMFGMHTLESLNRTKPDDVQRIGCVTRCKLQDAGHLHDDTTIAAAYEKKSRDDFERRNNYMRNDLDDKIKHIRKCANDLKADEKYDKCIYAYKMEKCVQRHGVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEQ2</accession>
    <name>T1GEQ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF3456 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the canopy family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEQ2"/>
    <dbReference type="STRING" id="36166.T1GEQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001823-RA">
        <property type="protein sequence ID" value="MESCA001823-PA"/>
        <property type="gene ID" value="MESCA001823"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078068_0_1_1"/>
    <dbReference type="OMA" id="GQDKACL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021852">
        <property type="entry name" value="DUF3456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15382:SF8">
        <property type="entry name" value="CANOPY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15382">
        <property type="entry name" value="CTG4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11938">
        <property type="entry name" value="DUF3456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588224" description="DUF3456 domain-containing protein" evidence="4">
        <location>
            <begin position="18"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3456" evidence="5">
        <location>
            <begin position="36"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001823-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="25034" checksum="899BE7BD4F0C6222" modified="2013-10-16" version="1">MLIRTIFISCLIATILAKEDSKNLAEKEHGVTYASKCETCKYLSMELTDRLSETGKTSEVLEIGYSVDDVKPKKRQEYKKSELRLIESLENVCERLLQYNLHKERSDSTRFAKGMSQTFETLHGLVNKGVKVELGIPYDLWDKPPVEVVQMKTQCEFMGKSSDNISLTDFLCRDRVLKKNDQECLDEKLVENEKGDNGKKQAKKDKKIKDKGRGEKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF37</accession>
    <name>T1GF37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001966-RA">
        <property type="protein sequence ID" value="MESCA001966-PA"/>
        <property type="gene ID" value="MESCA001966"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760733_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="1">
        <location>
            <begin position="18"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001966-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8199" checksum="ED2EF14C288DA0B2" modified="2013-10-16" version="1">MIGKQSFLGKFLEKMDLELSEAEILEIQNTWKIPMADPLASGQAVYLKLFKRYPSNQLKFIDFKVFVMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF66</accession>
    <name>T1GF66_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02188079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF66"/>
    <dbReference type="STRING" id="36166.T1GF66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001997-RA">
        <property type="protein sequence ID" value="MESCA001997-PA"/>
        <property type="gene ID" value="MESCA001997"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017286_4_1_1"/>
    <dbReference type="OMA" id="NIFWINI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF814">
        <property type="entry name" value="MEPRIN A SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="18333" checksum="E2A0EF98579825E6" modified="2013-10-16" version="1">MESDSKGLDGQMELYIRVTNYQDGCFSSVGYWEEVQQLNIGPFCEYEGTVIHEFLHAIGFFHQQSSYERDLFIDIIWENIEEGKDHNFDKRKIRDVTNFRTLYDYSSIMHYGESAFSKNGKPTMVAKFPEGQDQMGAREMSPTDIYKINRMYNCEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF72</accession>
    <name>T1GF72_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002003-RA">
        <property type="protein sequence ID" value="MESCA002003-PA"/>
        <property type="gene ID" value="MESCA002003"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888346_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002003-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7374" checksum="EEAEE42A8BFB912F" modified="2013-10-16" version="1">MPKDAWKFQNTRGPSQSNIIYKTADFAYKHLVSLIQEPHISATEYQQDYQLTTLNVKLLMIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF99</accession>
    <name>T1GF99_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF99"/>
    <dbReference type="STRING" id="36166.T1GF99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002035-RA">
        <property type="protein sequence ID" value="MESCA002035-PA"/>
        <property type="gene ID" value="MESCA002035"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="OMA" id="ICAYSED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF94">
        <property type="entry name" value="AT20289P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576876" description="Peptidase S1 domain-containing protein" evidence="10">
        <location>
            <begin position="20"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="22"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002035-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="244" mass="26871" checksum="96491D2C9597F191" modified="2013-10-16" version="1">MFRIILLLGSISSLFNVESKKIVGGTAIPIEKAPYVVSVQYNDYKHFCGGSLISKNLILSAAHCFEEMDPKGITFRLGSANSTSGGILIPAKKIELHEKYNSKPIKNDIAIILLNETVQKNSSHRASYKEPPQGSQTFASGWGVKKFGEKITPEELQGVYLDTISKKECQKAFGSTKIDDKTICAYSEDKDTCRGDSGGPLAWKAKLLGIVSWGIGCAKKHPGVYTSVPKHYKWIQKTKKILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFE0</accession>
    <name>T1GFE0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No mechanoreceptor potential B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02076261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFE0"/>
    <dbReference type="STRING" id="36166.T1GFE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002081-RA">
        <property type="protein sequence ID" value="MESCA002081-PA"/>
        <property type="gene ID" value="MESCA002081"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010738_1_0_1"/>
    <dbReference type="OMA" id="LHICGTI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="379"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="413"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="536"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002081-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="605" mass="69594" checksum="07439CB64A29CFC2" modified="2013-10-16" version="1">MFVEALNTYSIMTKDEMFKMFPHANQLKLNMGNIYSKMGRYKKAIKLYQMALDSVPIQHKQLRLKITQNIGTIFIKMGQYSDAATNFEIVLGERAEISCAIHLILCYYALGNVEKIKHAFKILVESQLYYEDDIKLQSTESQGHEDGSDENGNKSNKQIFEYLKNDEFAKYCNLRKKKAEKSISVIIDLISSVIADNYNDGYIWCIEVIKSSNFAWLANALELNKALVYLRQNDINQAVETLSFYEKKDPEMAVNALTNLMFIYISLNDLENAAKCTQKITVDIFEHPILLKMLLLWKCVEETGNNPGNYSRKQFYKQTNEVQLAAAVLNNLKNSTALAYTKHPHIFYQLANLCEHENESHEALELYLQLLGITNEMDAKLYAKVGEIYERFGEHQEANQYLNEAFRINPSNIQISTTLGSYFIKMQAVEKAIFYYERAVLSNPQDPVIQLRIAGCYRNILMPRQYLELFKKIYEKFPENLQCLKALVYVTKSQGLAELHEKYSIEMSRLEKKLMSRAPTALTDGNGNRNRKLSRAGSATSTASTTSYAEKSQFVSPEYVDPLGPKPERPRTGMTSSRKMKGYQRDSQNDDIDEDDNIILKIFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFF6</accession>
    <name>T1GFF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleosome assembly protein 1-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082813">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFF6"/>
    <dbReference type="STRING" id="36166.T1GFF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002097-RA">
        <property type="protein sequence ID" value="MESCA002097-PA"/>
        <property type="gene ID" value="MESCA002097"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038841_3_0_1"/>
    <dbReference type="OMA" id="AAECKQN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002097-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43853" checksum="F881998A81BF8C39" modified="2013-10-16" version="1">MSVAKHDNASDSDGEYKFSSNQFFSNALGFVTAAVRKQVTLEMVKALPKDVQDRITVLKNVQLQQLQLEAEFYEEVYALEKKFHEKFSPLFELRRSIVSGEKEPEKEAPKWTVTEEEVDEAFNEKFKEYKEEIHKNVKETVPEGSKGIPLFWLTLFKNTELLSEMIQDHDEPALKALQDIKLTYGEENSYTLEFYFGENEYFTDKVLTKQYFLKSMVDPESPFTYEGPEIYKCKGCNINWNKGKNLTVKTIRKKQKHKERGAVRTITKQVPNDSFFNFFNPPEVPENKDEIDEESQGILATDYEIGHFLRARIIPKAILYYTGDVVDDDDDDYDDEEEEEEEEEEEEEEESGDERPRQKRITAGGKKGANPNECPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFY3</accession>
    <name>T1GFY3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002285-RA">
        <property type="protein sequence ID" value="MESCA002285-PA"/>
        <property type="gene ID" value="MESCA002285"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239681_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12351" checksum="12AF256D0C26E384" modified="2013-10-16" version="1">MSKNIVSGFWINHGWAKQSIENIDFYEFLAFVHQYCSFRRTNLIHTSIVRGNQLQRRKEHYRTSRRLCMDEARLGFKPTECSGALAAKTSEKKINCYHCIASFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFZ8</accession>
    <name>T1GFZ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFZ8"/>
    <dbReference type="STRING" id="36166.T1GFZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002301-RA">
        <property type="protein sequence ID" value="MESCA002301-PA"/>
        <property type="gene ID" value="MESCA002301"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856325_0_0_1"/>
    <dbReference type="OMA" id="VPQIMNG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF7">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002301-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7254" checksum="8434F3F8142C390F" modified="2013-10-16" version="1">MDKSMMMPKRSRMDVKPFANGPPMQSRPLVALLDGRDCSIEMPILKDVATVAFCDAQSTSEIHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG35</accession>
    <name>T1GG35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02102194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02102195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG35"/>
    <dbReference type="STRING" id="36166.T1GG35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002341-RA">
        <property type="protein sequence ID" value="MESCA002341-PA"/>
        <property type="gene ID" value="MESCA002341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830197_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493">
        <property type="entry name" value="CUE DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493:SF0">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16052" checksum="32D29A4F42CB6A68" modified="2013-10-16" version="1">QIPNADISFIDDIVLSYIVSIIKEIEDDEFDLDAFQEMISAYFNDFATIDSGIVCAWLFKLEGELRQNAAVEEKEKDKKAEVQLASLKLSDIIPESKLKRRDSTAQEKEENATRNSRRSQHLSETSDAGSTDSSNYDYFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGE1</accession>
    <name>T1GGE1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084534">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGE1"/>
    <dbReference type="STRING" id="36166.T1GGE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002459-RA">
        <property type="protein sequence ID" value="MESCA002459-PA"/>
        <property type="gene ID" value="MESCA002459"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850170_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038905">
        <property type="entry name" value="ARMC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21356">
        <property type="entry name" value="ARMADILLO REPEAT CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21356:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002459-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15480" checksum="5305271241AA530D" modified="2013-10-16" version="1">MFFTKDNFVDCQDNFNKRLCVHGAIQLMVLHLQIINEFASINKLKGPPLHALYQLSAALRTLASISLMDSTFCSNNDDKDKYVGLDLACPHLIKAAEIAFGEVELQSNIVRTLSVFSENEACLEILSNFPDKIGILFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGW5</accession>
    <name>T1GGW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002646-RA">
        <property type="protein sequence ID" value="MESCA002646-PA"/>
        <property type="gene ID" value="MESCA002646"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877775_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002646-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="16083" checksum="3A5D2B89F5504533" modified="2013-10-16" version="1">MVQVKLYRSRTTGLKPTKLAVLKEKSSHSCTALSWYDTNDFFNLVPYHAIKLLVVPKNHFRSSKSFDLRRFSQVFFDPRRIHDDSWIYLDKETLLSLHSYLSRVDQLCLEAYTIDTVRNCTFIHSFTFRSKIKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHA6</accession>
    <name>T1GHA6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHA6"/>
    <dbReference type="STRING" id="36166.T1GHA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002796-RA">
        <property type="protein sequence ID" value="MESCA002796-PA"/>
        <property type="gene ID" value="MESCA002796"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064140_0_0_1"/>
    <dbReference type="OMA" id="CIGYGRK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002796-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13518" checksum="11682A76A06183FC" modified="2013-10-16" version="1">MASVFMRIFNLICMMLLIGHWSGCLQFLVPMLQGFPSNSWVSINELQEAYWLEQYSWALFKAMSHMLCIGYGRKAILKCCDMTVPRRQYKISSNLPICQGSIIQGSLPLTDIDLSVMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHM3</accession>
    <name>T1GHM3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02074368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHM3"/>
    <dbReference type="STRING" id="36166.T1GHM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002930-RA">
        <property type="protein sequence ID" value="MESCA002930-PA"/>
        <property type="gene ID" value="MESCA002930"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="OMA" id="HAFFNIA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002930-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14941" checksum="897694E18D5E75CD" modified="2013-10-16" version="1">MFSKQTLRKHSVLSFLIRKSVDDYIVPQTKSLIKKGTFIIIPVDAIHHDQDIYPDPEVFDPERFTPEAINSRPSCSYLAFGEGPRNCIGLRFGKMEIRAGLVSLLRKFKFSISDKPQYQFKEGSLIPQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHQ1</accession>
    <name>T1GHQ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002959-RA">
        <property type="protein sequence ID" value="MESCA002959-PA"/>
        <property type="gene ID" value="MESCA002959"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1405729_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022709">
        <property type="entry name" value="SCAI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21243">
        <property type="entry name" value="PROTEIN SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12070">
        <property type="entry name" value="SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002959-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="22403" checksum="D1F07E4096752C6E" modified="2013-10-16" version="1">MLILLPDGYELGGLATSVKRDPLESGISFRGKSYKEPHCLYPGDLYPFTRRPMFIIIDSDNSFVSNIFQDILGSHSLFLCRLRMYHQHFKVTDVQHHGSLFTLFLHSPLTALCYICNVGDVPIHHWERCQCYVDRFVTEASRLVTRCRIDEIEQGIGFIVNVVKITLQQEFRKVYSLLATFEADSYTGGEWEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHS7</accession>
    <name>T1GHS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHS7"/>
    <dbReference type="STRING" id="36166.T1GHS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002987-RA">
        <property type="protein sequence ID" value="MESCA002987-PA"/>
        <property type="gene ID" value="MESCA002987"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_11_0_1"/>
    <dbReference type="OMA" id="TIFEACM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF497">
        <property type="entry name" value="KELCH-LIKE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002987-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="283" mass="32520" checksum="F72E98FF70519137" modified="2013-10-16" version="1">MMEVENQSFSAHRVVLASSIPYFYAMFTNNMSESRTKEITMKEIDPESLETLINFAYSGKIRIDNHNVQNLMVGASFLQLNKVRDACADFLIEGFHPNNVLGIRHFADSMSCMHLISAADKYINLYFTKVSQCDEFLSLPFDELIELIRKDELNVTTEETIFEACMRWIKFDEINRSKQLPEVLARVRLPLLSPQFLADRVATEDLIRKSHKCRDLLDEAKDFHLMPERRALIFYKKYYFSGYDGESNLSTVEVYDPKTNEWVFVAQMCAHGGGVGAGVIPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI96</accession>
    <name>T1GI96_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI96"/>
    <dbReference type="STRING" id="36166.T1GI96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003165-RA">
        <property type="protein sequence ID" value="MESCA003165-PA"/>
        <property type="gene ID" value="MESCA003165"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146225_0_0_1"/>
    <dbReference type="OMA" id="TIKTCEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23599">
        <property type="entry name" value="TFP_LU_ECD_Cold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10036">
        <property type="entry name" value="CD59 GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10036:SF3">
        <property type="entry name" value="PROTEIN SLEEPLESS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577395" evidence="4">
        <location>
            <begin position="26"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003165-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16930" checksum="828E9F4EB72B9D10" modified="2013-10-16" version="1">MDFWKISITVLFGVYLCSQVSFVNGLECYVCSNQTGNTEKCLNTIKTCDAGEDVCFTEIRWGSQPYFSQGALKQYYVSKKCMSKHNCQRLRKRYTPYCTHIWYEDWTCSECCQGDRCNYFVISGSTRTSLGFVFILILPLWRIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ96</accession>
    <name>T1GJ96_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ96"/>
    <dbReference type="STRING" id="36166.T1GJ96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003540-RA">
        <property type="protein sequence ID" value="MESCA003540-PA"/>
        <property type="gene ID" value="MESCA003540"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3112416_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="51" mass="6055" checksum="0240911B2F68C2C9" modified="2013-10-16" version="1">QIIHGEVHSEPAHIIRKRSIDQPLRILLFYDDSMYRLDNEKFALINVTNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJE8</accession>
    <name>T1GJE8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S28</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02125497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJE8"/>
    <dbReference type="STRING" id="36166.T1GJE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003594-RA">
        <property type="protein sequence ID" value="MESCA003594-PA"/>
        <property type="gene ID" value="MESCA003594"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867441_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003594-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15595" checksum="F78785884568A726" modified="2013-10-16" version="1">MYWRNFGEIDGGKRTNFCKNFGENDGGSVQEEKKPVNCVIKVVRRTGSLGQRTQIKVEFLGEHNRQIMRNVKGPVREGDILTLLQSEREARRLQISEKSMVVLGSVLIVVSETKVHLCSGYLPIFTSKLHKKTLKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJG3</accession>
    <name>T1GJG3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJG3"/>
    <dbReference type="STRING" id="36166.T1GJG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003609-RA">
        <property type="protein sequence ID" value="MESCA003609-PA"/>
        <property type="gene ID" value="MESCA003609"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092179_0_0_1"/>
    <dbReference type="OMA" id="GTICLAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003609-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="244" mass="28392" checksum="CF15379BE59DDFE7" modified="2013-10-16" version="1">MNIIKINALIKEELFAESSIHGVQYINKNELHWTERVLWLAVIVSATAYTGVICFQQWNRFRTNPIVYAVEINSNTREIPFVAITMCSNYSNRQTNTEIIQKYFQITESNDEFLYYMDFLNTLKDVDYSNMQVLESYAQNMKIRSVSLLDIAIDIKKPLVQERETAVEQQQQSKPRYNMIITEMGVCFSTSESANQYQNPNRLVKVNTTSQHDICRPLDVCTLKTAPFVSDAAMVKFFFLMRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK44</accession>
    <name>T1GK44_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165076">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003859-RA">
        <property type="protein sequence ID" value="MESCA003859-PA"/>
        <property type="gene ID" value="MESCA003859"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852279_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003859-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7760" checksum="6F496715D15F0BCA" modified="2013-10-16" version="1">MYIYEGVIVSKNDFHASKHPEWGQISNLQEIKELQNNRLKKSVIYMQKIHFTISVTHWNPNDMDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL05</accession>
    <name>T1GL05_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004188-RA">
        <property type="protein sequence ID" value="MESCA004188-PA"/>
        <property type="gene ID" value="MESCA004188"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212952_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12414" checksum="BF57A4CC63F6F14D" modified="2013-10-16" version="1">MEVDNLFILETNKNPVLVFADKIYIRYLYRVIEDCNSACRLRNFPDKEKEFHSIFGNHILQDQLKFRNELIMTNAFYSSVLKTLVTQKMSSAGKGCGRLLASRCTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL29</accession>
    <name>T1GL29_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vitamin K-dependent gamma-carboxylase</fullName>
            <ecNumber evidence="2">4.1.1.90</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Gamma-glutamyl carboxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Peptidyl-glutamate 4-carboxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Vitamin K gamma glutamyl carboxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-carboxy-L-glutamyl-[protein] + 2,3-epoxyphylloquinone + H2O + H(+) = phylloquinol + L-glutamyl-[protein] + CO2 + O2</text>
            <dbReference type="Rhea" id="RHEA:45140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:11094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15759"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28433"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:84990"/>
            <dbReference type="EC" id="4.1.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:45142"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.1.1.90" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02042702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL29"/>
    <dbReference type="STRING" id="36166.T1GL29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004215-RA">
        <property type="protein sequence ID" value="MESCA004215-PA"/>
        <property type="gene ID" value="MESCA004215"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020495_0_0_1"/>
    <dbReference type="OMA" id="TYLNHYY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008488">
        <property type="term" value="F:gamma-glutamyl carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019842">
        <property type="term" value="F:vitamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011020">
        <property type="entry name" value="HTTM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053934">
        <property type="entry name" value="HTTM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007782">
        <property type="entry name" value="VKG_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053935">
        <property type="entry name" value="VKGC_lumenal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12639">
        <property type="entry name" value="VITAMIN K-DEPENDENT GAMMA-CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12639:SF6">
        <property type="entry name" value="VITAMIN K-DEPENDENT GAMMA-CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05090">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22777">
        <property type="entry name" value="VKGC_lumenal_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00752">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="HTTM-like" evidence="16">
        <location>
            <begin position="30"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004215-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="608" mass="71218" checksum="D4DF4FA8A4831A33" modified="2013-10-16" version="1">MRHFKKVVESFTGHNWNHFHSLDNFILWLHRAVDASALGIFRFFFGLLMLIDVAEERGGSSLDVRYFQDGICQFPLFPFIRPMNVPQMGVVYGVIGLELLELCWDTDFESPALCTRFAIGISCYSTRLLGTITVTCLDCVVFCFSSIESLFSNDIPQKVPYWNYFLIKFQFFILYFIAGLKKLSVEWLSGYAMTNLGHHWIFSPFRIFFTVETTELIFIHWFAAIFDTSIAFLMIWEVSRNYVLPFMLSFHLMNSRLFEIGMFPWVCLAQVQLFYPRDWMKRKKSNEKIEICEKRNATPREKRTTLFIALYCGLQLFLPYSHFITKGYNNWTNGLYGYSWDMMVSSYDTIATSIKIIDNTNNEAHFLEPFAFTEYDRWTKYADMAKQYAQCISDKLTNTKDYGNPLKSGTYQYILIFQQRVFDPNVDILKAEWSPFKETKWVFPLLNQFNDMRPKIESLASNVYSWSNYSDVLFIADFPGLTIENFISTDLDNITIHVLEGKVEVHNGNETARNLISGESLKITSGQTHFVKTVSETPSTFLYTYINRTMLEMGNIEDFHADISKPNINPYKEFMERVDNYKKFLYNVGNSILFLIYKVPMPLRIREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL33</accession>
    <name>T1GL33_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL33"/>
    <dbReference type="STRING" id="36166.T1GL33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004220-RA">
        <property type="protein sequence ID" value="MESCA004220-PA"/>
        <property type="gene ID" value="MESCA004220"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514285_0_0_1"/>
    <dbReference type="OMA" id="RDSIMIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF63">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004220-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="20166" checksum="79E432AA752E2D69" modified="2013-10-16" version="1">IILNLNFSSRTSSLDVQRTIEAAVEKRTKDTFGPPIGKRIACFIDDMNMPQVDDYGTQQPIALLKLLFERGGMYDRDKDLIWKKFKDLTFYSAMGCAGGGRNEVDPRFISMYSVFNLVFPNDESLAQIYSAILKGHFVKGGFKENLLKICDILVEMTLKLFKGVKKTFTMGTMPTTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLH3</accession>
    <name>T1GLH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore complex protein Nup214 phenylalanine-glycine (FG) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GLH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004375-RA">
        <property type="protein sequence ID" value="MESCA004375-PA"/>
        <property type="gene ID" value="MESCA004375"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_247466_0_0_1"/>
    <dbReference type="OMA" id="WLSTFQF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026054">
        <property type="entry name" value="Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193:SF46">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1288"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1428"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1504"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1288"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1334"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1509"/>
            <end position="1520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004375-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1534" mass="160616" checksum="A89171F4D3D605E5" modified="2013-10-16" version="1">MALNCPESKDVLDFQFKIQGKFEVFKKLDQELKSNVNLLAVASNHGLLFAGSICAPEFKVLQHKNLVNFRETNEKPVARLVSIPTPPETLAASCDGSMLGVAYNQNGAAFLAIYSLPSFLTPNIQQLHTIKLCPEDNVSCKELVWNPVIPNILAIILSNGSLVMYSFKSNSYEFNALDKKEQCFCACWSPKGKQIVVGSANAIECPPGVHGTPFKVISILWLSTYQFAAVFLQDGGDPCLHIINAPKAGPPSYINYFDICYGGGIRENQVFLTYIQSWNIFLVTSANSAEVGVMGTTETGEAPTWSQYILEDVGRIELPLTEDKEDVFPIGFVLDTASSHRVVIDEQEKPVMPMVHILSTHGTLISYNCMNLKPNAPDLCSPPPPINDTTGGQFKPLQDLVTSSRATSVPPHVDMTFTIPKGSTSTPAKERPSIGPNTTIFGGGLGGGIGFGQPTATKLNFGAPAATMPSLSFGTKPAESKPIGPTIPLAKPQPTQSSGLQLSFGQPQTQQAVQKPAATTNQPLYTVQPTYQPPTVQSSAPIPTTVSKIHDDNDLDDIIKKMVVDQIIAFDSEIKLNIKKTSDISSKINNLEEIKSYARRLAKLDEINQQAEEDEFTTDIQTLRHSINESYNIIIECKTKLENYKNPDLAKLTMKTISSNANTRQIAKLDSLLATNRAQIHVAQQLLRRNLSNINKAEGYLATERIKINCIKAKLNSKEFTELKDNANVDAESLADSILSMTLNPEIKESKLSQEKLRNIKEALKDRPVRIIKPSRPDRVGLKSEIILETKIKEQKAKKIVPKKAIEEPVKPISEPVVPSAISFTPKVVGTEVTIPKSLTFAPLQTKPVEKAKDTTTTFSFAPKEPPKQITFGEKPKENVSPAISFGLKASTASQVSLPTEKAKESPIPSFGVKPTVSSAPNIPFGGSFNFNTSKPAPIPTSDFNPISKAQPISFVTPQTLKPEPEKPKEINKPETIAPKDETKVPLNFSLSVTATSSITSSAATTTTTVAPPVKADETDNLFGNLTICKPTSVSDKKPESNIWPSLATSLETSKEEKTSLFSGSSVASPFSMKPTSTASPFVAPSAASPIFGQTTTTTVNISSAPASSTPVSAPASNPSFTQPAVSSTASVAASSTATPSVFGQSTFATTTASSTVSSVASPFASSSTTTTSAVSSVASPFAPVFTTSAIASPFGQAAVTATTVASPFAQSTATTSSQSMFGQSTAVTSTISSPFASGSPFGQTSVASTGSIFGANAQIPKPDQSLTNYIKSGGNIFSSGFGSPQASSGGSIFGGNQSQTAGSPFGTPSATANTGSIFGQTSSAPNQGGSIFGGNTQQQQQQQASGGSIFGGSAFGQTTGQSSGSIFGGGNAFGASPSNVGSPFGAQASSPSSPFGGVQPSAFGGSTSFGGGGGSVFGGSAFSSGGSSIAQSGFGSPQAPTQQPAFGAAPAFGAAPTFGGGATFGSPKGFGSPLANTQTSAFGSPTQKSNLFESLGSQGSGIGFGSLAQQGNAQNNNNNKPAFGGSAFTSYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLZ1</accession>
    <name>T1GLZ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02102396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLZ1"/>
    <dbReference type="STRING" id="36166.T1GLZ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004557-RA">
        <property type="protein sequence ID" value="MESCA004557-PA"/>
        <property type="gene ID" value="MESCA004557"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_4_2_1"/>
    <dbReference type="OMA" id="ELETRWM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000084">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF140">
        <property type="entry name" value="FI02848P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="11">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004557-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="23622" checksum="4B6CF107D080E766" modified="2013-10-16" version="1">MPLINADGYEFTRSKSRNRLWRKTRKPYKNCIGADPNRNFDYKFGFSGTSTDECSNKYQGPEPFSEPETAVLRDVLKTYNGRISFYLSLHSHGEYLLYPWGYDKIDASNKNELEDVSEAGSQAIFELSGRKYKVGDMANTLYPASGTSQDFAYHEGARISITMELPSGGPAKFNPPPKDIRKYAEESWIGIRAMASKVIEMYSKKSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM87</accession>
    <name>T1GM87_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02193620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM87"/>
    <dbReference type="STRING" id="36166.T1GM87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004655-RA">
        <property type="protein sequence ID" value="MESCA004655-PA"/>
        <property type="gene ID" value="MESCA004655"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1580308_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004655-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="19314" checksum="5315108B422A4447" modified="2013-10-16" version="1">MNKEEQFNLKWAGHHSEILSGIGNMINSNIFVDCSLSAEGKSLKAHKIILSACSPYFAVKLQATLSYIYNGEVTVTRNFLDSFVKVAKTLQIKGIYDHSSTNKDPLNMKECAQEKIHMEESVPESYNNMKNPEIKSPISLIQKDSKMFILLRFQQREHQIVPKFLILIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMB7</accession>
    <name>T1GMB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMB7"/>
    <dbReference type="STRING" id="36166.T1GMB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004687-RA">
        <property type="protein sequence ID" value="MESCA004687-PA"/>
        <property type="gene ID" value="MESCA004687"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1262832_0_0_1"/>
    <dbReference type="OMA" id="MWITDEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030299">
        <property type="term" value="P:intestinal cholesterol absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004687-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24774" checksum="189494F73FB2D0DC" modified="2013-10-16" version="1">MAYHSILKTSEDYYSALREARKISQNITHMIQGRLIQQGLSIEEAKQIEVFPYSVFYVFYEQYLTMWPDTLQSMGISVLAIFIVTFLLMGFDIHSSIVVVITITMIVINLGGLMYYWDISLNAVSLVNLVMAVGISVEFCSHLVHSFSISIQTTREKRAADSLSQMGSSIFSGITLTKFGGILVLAFAKSQIFQVFYFRMYLGIVFLEVIVFNGEDDLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMD8</accession>
    <name>T1GMD8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02110347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMD8"/>
    <dbReference type="STRING" id="36166.T1GMD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004710-RA">
        <property type="protein sequence ID" value="MESCA004710-PA"/>
        <property type="gene ID" value="MESCA004710"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_10_6_1"/>
    <dbReference type="OMA" id="FWFYEIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000038">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF13">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="9">
        <location>
            <begin position="296"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="506" mass="56842" checksum="5362F2BDAF6394FD" modified="2013-10-16" version="1">MGDVMAIRKRNPKLAEIPFNSTNKFQLSIHGIDDDMRYLLVMKGAPERILERCSTIYINGEDKPLDDEMKENFNKAYMQLGGLGERVLGFCDYILPTNKFPKGYKFDTDTVNFPTSGLRFVGLISMIDPPRAAVPDAVAKCRSAGIKVIMITGDHPITAKAIARSVGIISEKSETVEDVAERMKIPVEDVDPRLAKACVVHGNDLRDITPENLEEILRYHTEIVFARTSPQQKLIIVEGCQNMGSIVAVTGDGVNDSPALKKADIGIAMGIAGSDVSKQAADMILLDDNFASIVTGTDLVPAISLAYEKAEGDIMKVKPRNPFKDSLVNSRLISMAYGQIGVIQAAAGFYAYFVIMAENGFLPRRLFGIRKLWDSKNVEDLKDSYNQEWTYRDRKVLEHTCNTAFFVAIVVVQWTDVIICKTRRLSLFHQGMSNWALNFGLLFETALAAFLCYCPGMDKGLQMYPLKFVWWLVAVPYSITIFIYDEIRKYFIRKYPGGWVEQETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMX1</accession>
    <name>T1GMX1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02059917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMX1"/>
    <dbReference type="STRING" id="36166.T1GMX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004905-RA">
        <property type="protein sequence ID" value="MESCA004905-PA"/>
        <property type="gene ID" value="MESCA004905"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102430_0_0_1"/>
    <dbReference type="OMA" id="GMDFDPN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="96"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004905-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="237" mass="28403" checksum="A63AD57E4D5843FE" modified="2013-10-16" version="1">MGKTRKKQKVELVFDEQKRKDFLTGFRKRKNERRKKAKEQVEAMIKEERKRIKSELKAGMKNMKKSFQPLQELTEEDKKQEYEDDEVEVKIVELSANDFAAQRNMIGANTGQESEEEEEPEEDEEEEVDENTIPGMDIVEKKKKRRKPTSEDQDDSKKKSKTEHEFKSKKDIDKVMKKKTLRNLKKTSAFKQKEKFEQVANRKKARREKRNTIKSLPKHKQKEKKRFNNDLNRKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN39</accession>
    <name>T1GN39_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02150917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN39"/>
    <dbReference type="STRING" id="36166.T1GN39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004978-RA">
        <property type="protein sequence ID" value="MESCA004978-PA"/>
        <property type="gene ID" value="MESCA004978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005746_7_2_1"/>
    <dbReference type="OMA" id="HCFFQED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005221">
        <property type="term" value="F:intracellularly cyclic nucleotide-activated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000004">
        <property type="entry name" value="Cyclic nucleotide-gated olfactory channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF7">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL-LIKE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="145"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="233" mass="26264" checksum="691507DD9010C855" modified="2013-10-16" version="1">MLFQTHVSKSSSSRGRIDVRNLSLLSPFMSIRPLQRRYVFTIVSYLIGVFIFATIVGQVGNVITNRNANRLEFERLLDGAKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGGGDINTALGLLPDKLKTELALHVNLSVLKKVTIFQECQPEFLHDLVLKMKAYIFTPGDSICRKGEVAREMFIIADGILEVLSETGKVLTTMKAGDFFGEIGILNLDGLNKGERGFVGIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN45</accession>
    <name>T1GN45_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN45"/>
    <dbReference type="STRING" id="36166.T1GN45"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004986-RA">
        <property type="protein sequence ID" value="MESCA004986-PA"/>
        <property type="gene ID" value="MESCA004986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451125_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050211">
        <property type="term" value="F:procollagen galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF53">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="22094" checksum="8F7C0B969E7A396A" modified="2013-10-16" version="1">MLSAHSTKHDLRRIKEVVGICGESESSTRISLFWSHRVYYMKREIRSDHNEDDSISITRLWLKRVEPLYHNNRVHWSNDRFAQIIAFKEEAFQFARKCNTDFVFFCDVDVLLTYPKTLDILIKLNLPLVSPMLVSANQLYSNFWALEEYYIIIQSHYVGEFNVSIVHSAVLVNLNIQETKFLAFDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNF3</accession>
    <name>T1GNF3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNF3"/>
    <dbReference type="STRING" id="36166.T1GNF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005103-RA">
        <property type="protein sequence ID" value="MESCA005103-PA"/>
        <property type="gene ID" value="MESCA005103"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1268212_0_0_1"/>
    <dbReference type="OMA" id="NTHICTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005365">
        <property type="entry name" value="Npr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153">
        <property type="entry name" value="CGTHBA PROTEIN -14 GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153:SF5">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="24583" checksum="46CCCE4A0520368B" modified="2013-10-16" version="1">MEVNPVSVILVKADSKGDRLLFRYPYEVQKNDISSKTNEIKKNPYAVSITDDVLQNTPPPASNIINGELRGFTDEVLSTLFAVKPELTNQKFELKVNDVRFVSHPTQMQPKDDSNGSILINVVFALHAQASYSIVKCYYELSKKLGYALKMEEERVAYLTEEMGIMVKTHDEVANVIEQKKSLINVCEFDLILERSSLAQSLKSIYHDLCTTGLSTFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP22</accession>
    <name>T1GP22_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183548">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005338-RA">
        <property type="protein sequence ID" value="MESCA005338-PA"/>
        <property type="gene ID" value="MESCA005338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944357_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6597" checksum="91B9BD50065B8322" modified="2013-10-16" version="1">MNTEIGTHSIHPIEVISSTLKRTKTPESSSCKRSVFCPFGGSEATDNLAWINPLPKVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP70</accession>
    <name>T1GP70_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lethal giant larvae homologue 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02135399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02135400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP70"/>
    <dbReference type="STRING" id="36166.T1GP70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005387-RA">
        <property type="protein sequence ID" value="MESCA005387-PA"/>
        <property type="gene ID" value="MESCA005387"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582575_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013577">
        <property type="entry name" value="LLGL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08366">
        <property type="entry name" value="LLGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lethal giant larvae homologue 2" evidence="1">
        <location>
            <begin position="100"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005387-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="18592" checksum="188E494230782DA9" modified="2013-10-16" version="1">RTRSTRPGAVVGLCDSPIDANKLLIAYDSGQIVLWDLRGKFGEMRWQSAEPLKSVAWHHEGKHFVSSHTDGSLCTWPLRPSPKPQIHSFPHAKTNKDGKLEACKPIHKVDLRTAKTGETFTIFSGGLSMEKGGKSPCITVLQGRSTTVLEMEHPVVDFVTLCESPWSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPW3</accession>
    <name>T1GPW3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPW3"/>
    <dbReference type="STRING" id="36166.T1GPW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005658-RA">
        <property type="protein sequence ID" value="MESCA005658-PA"/>
        <property type="gene ID" value="MESCA005658"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057784_2_0_1"/>
    <dbReference type="OMA" id="HYSHWIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="184" mass="21371" checksum="63BF9864C4C51819" modified="2013-10-16" version="1">MRWNFRQNTNPLHNCLFDALFGSGTTYISLYNSLMKLIFLAASGATVYMMYVKFKATYDHNHDSFRIEFLLAPAAILALLINHEFTVMEILWTFSIYLEAVAILPQLFLVSKTGEAETITSHYLFALGSYRALYLLNWIYRYYSESHYDLIAIFAGIVQTILYCDFFYLYITKVLKGKKLQLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ05</accession>
    <name>T1GQ05_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GQ05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005702-RA">
        <property type="protein sequence ID" value="MESCA005702-PA"/>
        <property type="gene ID" value="MESCA005702"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778773_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005702-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7895" checksum="5A84718572110A11" modified="2013-10-16" version="1">MDTSNMKSMPWFGIDIGGTLTKLVYFEPKDITPDEKNAEANSLRNIRKFLTKNSSYGNTXXXXXYFINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ27</accession>
    <name>T1GQ27_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02031865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ27"/>
    <dbReference type="STRING" id="36166.T1GQ27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005725-RA">
        <property type="protein sequence ID" value="MESCA005725-PA"/>
        <property type="gene ID" value="MESCA005725"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1456030_0_0_1"/>
    <dbReference type="OMA" id="ILSEYWN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005725-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="21397" checksum="437D04620D548DC5" modified="2013-10-16" version="1">MEKQTIDDVETKLEALFAESPGKSKTPSTDENVEQKTPEKQNPKIEKEPQTKPAVKKRRRKAKQKSQIKKKFAQQDQEKAQITNKFKKQQHQKPPEDSYKGPFIQFKKDGFFTVVNSSTAVDEDSEKQGSSKSKKQIYDKTKIRSFNVSTLSHKYDADKTDKTWVCVYCKNGPHKQNLVICLGLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ63</accession>
    <name>T1GQ63_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Doublecortin-like and CAM kinase-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02016863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GQ63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005764-RA">
        <property type="protein sequence ID" value="MESCA005764-PA"/>
        <property type="gene ID" value="MESCA005764"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_94_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Doublecortin" evidence="14">
        <location>
            <begin position="52"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="282"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005764-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="414" mass="46094" checksum="F15582A87C46A026" modified="2013-10-16" version="1">MEVTTAVAAAIANHQQLVNNHSSPSHSFQQGSSNCIVITLSQNNSPASSNSENGDKFFSGITIPVSTDRYRSFESLAEDLTKLLEDNVKVPGAVRNIYSSQGKRVSVQSGSCXDGQYYVCCCNNEAFKKIEYNISSNQQVTNKLNNRLSKYRPSSPMKCILNGSNSLAGNSLNGVSPNISLIERDTVVHPRIVTLIRNGTKPRKIMRLLLNKRNSPTAIQNTRKPLRNGTTSGPIPKMPGKSKIKVNHSVSIIQDNFEELVQIALENGIDIKDLPQEIRDKYALESIIGDGNFAIVLKVNDRQTKKPFALKIIDKSKCKGKEHYIEAEVRVMKKLQHKHIISLIQSVDQINNMYLVVEYIDVWAAGIILYILLCGFPPFVSPDNQQEPLFDAIISGVYDFPEPYWTGSVMKLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR67</accession>
    <name>T1GR67_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-glucuronosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR67"/>
    <dbReference type="STRING" id="36166.T1GR67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006143-RA">
        <property type="protein sequence ID" value="MESCA006143-PA"/>
        <property type="gene ID" value="MESCA006143"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012949_2_1_1"/>
    <dbReference type="OMA" id="ERETIHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577573" description="UDP-glucuronosyltransferase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33556" checksum="6A4E8A90296D41D0" modified="2013-10-16" version="1">MQILSLSLFTLCFFFSYRKYIFNPKIEALVRPHIAADCPPLTEIEKRTVLALVNSHPVVNYAEPLPPNVIEVGGLQSHDPKPLPKRIQQFIDDSQKGAILFSMGTNVPPEIFGEKGLSDILKVIEEMSDYNFLWKLDIQNGTLIFPKNLLVEKFLPQSDILSHNKTVAFISHCGGLSTFEATKYGVPIIGVPFFIDQFRNLRKAITDGAAVELPLKEFNARNLKSKVLEVLKNPAYSENMKKRSKRLNDQPNKPLERAVWWIEYILRNPEPDHLLSPAIDMGYFAAYSFDVIGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR94</accession>
    <name>T1GR94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02142495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR94"/>
    <dbReference type="STRING" id="36166.T1GR94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006172-RA">
        <property type="protein sequence ID" value="MESCA006172-PA"/>
        <property type="gene ID" value="MESCA006172"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_412386_0_0_1"/>
    <dbReference type="OMA" id="PRIFTIQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF498">
        <property type="entry name" value="DACHS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="576"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="666" mass="74786" checksum="2E8AD06A0430DB38" modified="2013-10-16" version="1">MTPRGHFIEVQVTDGALYRTKIHCYFLDQTRVIRPLSNEKNYHIFYQMLAGLSREERTKLNLDGYSAVNLRYLQNGITSQDDEEDSSRFQAWKTCLGILGIPFLDVVRVLAAVLLLGNIQFIDGQGLEVEVKGETELNSVANLLGVQAQFLFRGLTTRTHNARGQLVKSICDANMSNMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQGDAASHHASTIGESCRDEGIVCDAEVDYIDNVPCIDLISSLRTGLLSMLDTECSIRGTAESYVTKIKVQHTNSSRLEKSVNKMNDRHDPRIFTIQHFAGKVEYDATDFLDTNRDVVPDDLVAVFYKHTCNFGFATHLFGSELKALYNVENAPRGLSFRISPTSHTDLVNGDEQTLVHARPHFVRCIRSNSSEISGNFERASVVKQIRALQVLETVNLMSSGYPHRMRFKQFNSRYRMLAPFNVLSRLEEKAQENNSIILQYALGTKSAKLIEEFSNISLNYAMGKRHIFLSEGIRQHLEKLRAEIRSKSASLIQSVWRNWQLKKCGIIKPRLKDRTETELPTKVGKSINLTSGNAFKSPKVPNAQGTITRPRPQPIAGTPDPPDLNEKCDSKIIQQTCTMFGLDLVRFYFYILGMCEFLAPNEGIIDVPSGRFHLIDQVSSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSC2</accession>
    <name>T1GSC2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02096337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GSC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006575-RA">
        <property type="protein sequence ID" value="MESCA006575-PA"/>
        <property type="gene ID" value="MESCA006575"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_740314_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006575-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="374" mass="44098" checksum="F7EFDBAC5BC1A364" modified="2013-10-16" version="1">MDHIVKERYPAFIDTIKDLEDCLEMMFLFRTFASLILIPRVQSALCRCLTTVFLHYIIASKSLRKVFIPIKEHYFQADIKGHKVTWIIPHYCPFKPTTKVEVYFKELKFFNTTEVPREPLVIIIRSFGGKYPGIRTVILEEFMRKEMSVLHTTSLTDLAWRNNTFPEIMFNRRDFSTQNQKKLWSISKYLIGAELPPHLSPFMDHKRKTYVPPEEMALRDPSLIETHEQREEESVDEEEEEEPMNDQDLEKALQAERDQLDDSEDDEDQEMEDDEDEDLDENEKKXXXXXXXXXXXXXXXXXXXXDENEKKRKQEKKNMAVSAGKVNKRAIKKNEIEEHRLKARMLMNKKRKKIDAETKLKKKAEKKAANKAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSL5</accession>
    <name>T1GSL5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSL5"/>
    <dbReference type="STRING" id="36166.T1GSL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006675-RA">
        <property type="protein sequence ID" value="MESCA006675-PA"/>
        <property type="gene ID" value="MESCA006675"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963422_0_0_1"/>
    <dbReference type="OMA" id="AAHRQCP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006675-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6962" checksum="C0DE3E935DEFF295" modified="2013-10-16" version="1">MRDIRYKWNEGPNSVGVSSEVSLPQFKVLGHRQRAMEISLTTVSIFYDFVMNLRFTFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSP4</accession>
    <name>T1GSP4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSP4"/>
    <dbReference type="STRING" id="36166.T1GSP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006704-RA">
        <property type="protein sequence ID" value="MESCA006704-PA"/>
        <property type="gene ID" value="MESCA006704"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827517_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006704-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15289" checksum="3E46B21F8F8764CD" modified="2013-10-16" version="1">MLSAMISKMAISSTLSMLTTCTVELVSDEKKKICAFSTIVWARIWLLTAPFVGATIVFGQLVPQTAFASLSILGGILTMMISSPRTIPKKKTHIDLSTDSNYNANIQMGNNTPFKKDKSDLPPPLAPGIWTTKSQDIGARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT76</accession>
    <name>T1GT76_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT76"/>
    <dbReference type="STRING" id="36166.T1GT76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006899-RA">
        <property type="protein sequence ID" value="MESCA006899-PA"/>
        <property type="gene ID" value="MESCA006899"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028286_6_1_1"/>
    <dbReference type="OMA" id="FQVAVWG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF19">
        <property type="entry name" value="MITOCHONDRIAL AMIDOXIME REDUCING COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="148"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006899-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="299" mass="34122" checksum="8FB3741A0A2BE20B" modified="2013-10-16" version="1">NSKLPIILATGVGLLTVSGCYYLYKRYQYHYVPPKQWRKVGDLSDIICYPIKSCGPVIKTKDGKFITARQYPKMVKIQPKIEGKTMTLSAPGMMDVEVDFDRLYKTKPIKCSVWDQPVEAVDCGEDCARWFSRYILEDDFGLRLVFYARELSMRDVRPYNKCVDTTLREDTGAFHDATSFMILNESSLIDLNTRLSVPVIGLNFRPNFIVKGALPYEEDYWKWVKIGEVVFRSVKPCTRCIFTNIDPMTALRNENGNPLNVLKTYRMFPNLGSSPVLGTHLGLRVPGKINVGDEIFVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTA8</accession>
    <name>T1GTA8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006934-RA">
        <property type="protein sequence ID" value="MESCA006934-PA"/>
        <property type="gene ID" value="MESCA006934"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500492_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012587770" description="Secreted protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006934-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9758" checksum="241C9B0BD5CB2620" modified="2013-10-16" version="1">MVILIVNILIILILCIEFRTIPCSNQSRPRKGTINKTHKTDMQKVLEKSSSPPLKRSDGQEKRMLRIEPDINATTSGVTLRNMILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU86</accession>
    <name>T1GU86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02061489">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU86"/>
    <dbReference type="STRING" id="36166.T1GU86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007295-RA">
        <property type="protein sequence ID" value="MESCA007295-PA"/>
        <property type="gene ID" value="MESCA007295"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027228_5_2_1"/>
    <dbReference type="OMA" id="HEANVPP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000328">
        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF157">
        <property type="entry name" value="DPR-INTERACTING PROTEIN EPSILON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007295-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14727" checksum="6B206C3F9A03689D" modified="2013-10-16" version="1">LFPFTNRLKFSFNLSTVPPNIDDSLTSSDVIAQEGDNVSLKCKANGSPPPSLKWKRDDGSRMVINKSLEVNEVEGELLELERISRLHMGAYLCIASNGVPPSVSKRIKVSVDCEYIYISIYNKIIYTLRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUR4</accession>
    <name>T1GUR4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUR4"/>
    <dbReference type="STRING" id="36166.T1GUR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007483-RA">
        <property type="protein sequence ID" value="MESCA007483-PA"/>
        <property type="gene ID" value="MESCA007483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1327720_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="21573" checksum="242829D5252FEB28" modified="2013-10-16" version="1">MEGCSAPLVVKFADTQKEKEQKKLQQLQIGLWNAAAATGPAAVPVAVPQQSANSTNTLHHPVVQTIPTSAAVAPTQLAQNVGTLTTSQPLIAANPPTQTTQFLATDATTPLHLFHQLPGLHQPQLLQGLNPPDLSAFTAATGNGLFSIPNLLTLASFNQHHHNNNLQHQHTPQHSSAAAAALAAAAASGMQQHQTIPHNAASALARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV23</accession>
    <name>T1GV23_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV23"/>
    <dbReference type="STRING" id="36166.T1GV23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007602-RA">
        <property type="protein sequence ID" value="MESCA007602-PA"/>
        <property type="gene ID" value="MESCA007602"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005825_3_0_1"/>
    <dbReference type="OMA" id="KNTHESD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000003">
        <property type="entry name" value="Pre-mRNA processing factor 40 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000002">
        <property type="entry name" value="pre-mRNA-processing factor 40 homolog A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25432">
        <property type="entry name" value="FF_PRPF40A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="328"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="103"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007602-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="431" mass="49853" checksum="049FFB7086C1CA58" modified="2013-10-16" version="1">MSTDKMNSQLKYYKCEEVFGSMKIWSSVPEPDRRDIYDDAIFNLAKREKEEQRVAKKRNMKVLGELLESMANVTYSTTWSEAQVMLLENASFKNDVSLLGMDKEDALIVFEDHIRMLEKLEEQEKEREKKRAKRQQRKNRDAFLTLLDSLHEEGKLTSMSLWVELYPLISADLRFSAMLGQSGSTPLDLFKFYVENLKSKFNDEKKIIKDILKEKQFIVKADTSFEEFATAVCEDKRSANLDAGNVKLTYNSMLEKAEAVEKERLKEESKRVRKLENEIKQMWLDANLTSADSYETAKKHVQDLEAFATYEGNILQFRRFGKSLLRKVKTSKKSKKNKKHKKRVRSSSRSSSYESDRGSDVGSPLEEIPHKKKKKDKKKKDRKRSLSPEGISNTSLPSNGSSRLHEEQALSESELVSKRAALLAQLNEQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVG8</accession>
    <name>T1GVG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02168889">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVG8"/>
    <dbReference type="STRING" id="36166.T1GVG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007771-RA">
        <property type="protein sequence ID" value="MESCA007771-PA"/>
        <property type="gene ID" value="MESCA007771"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077576_0_0_1"/>
    <dbReference type="OMA" id="TICSHHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058796">
        <property type="entry name" value="COPS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25983">
        <property type="entry name" value="COPS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007771-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="18206" checksum="2FD18817B27AAF37" modified="2013-10-16" version="1">MNSAINPFDSQEAKPYKNDPEILAMTNLVTAYQNDDINEFESILRLNRNNIMSDLFIREHIEDLLRNIRTQVLIKLIRPYTKISIQFISKELNIEPSEVESLLVTCILDNTIQGRIDQVNQVLNLDKETRCAPRYNAIDKWSNQISSLQLAIINKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVP1</accession>
    <name>T1GVP1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007854-RA">
        <property type="protein sequence ID" value="MESCA007854-PA"/>
        <property type="gene ID" value="MESCA007854"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779552_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007854-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="15707" checksum="116C80D5EDF52F1C" modified="2013-10-16" version="1">MIMLPINLSFILYTKNLLIMRIKRVPPTTTTRSSLERERQQKQNKSKASGSKSKSTKSSASKSSGESTKKKSKPVAKSSKEPAQPSTNPKSSVDTNLPSSTKGNEKSKEQQASTSSSAAATSSTGQPSGEPRFLVQHPQMPEVLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWJ3</accession>
    <name>T1GWJ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWJ3"/>
    <dbReference type="STRING" id="36166.T1GWJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008170-RA">
        <property type="protein sequence ID" value="MESCA008170-PA"/>
        <property type="gene ID" value="MESCA008170"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402183_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008170-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10113" checksum="711744D15A4E408B" modified="2013-10-16" version="1">MKALRIGELLAPHNTTQNLHNTNFHVLSPPQSNPYGLKDSGATSDMTAWTSAGLQPTSSYYPYDHSLAAYGYSLGENDCVTLVWTCGDYIASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWW1</accession>
    <name>T1GWW1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWW1"/>
    <dbReference type="STRING" id="36166.T1GWW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008294-RA">
        <property type="protein sequence ID" value="MESCA008294-PA"/>
        <property type="gene ID" value="MESCA008294"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097304_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008294-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="18733" checksum="DE67EC528A20D7BF" modified="2013-10-16" version="1">MSIKHPSDSIRRPTSNSGVWTWLSNRRPSDGPDQLGPPSSSPAENHYTHMDDAYSPVGVVGEALYAELDRESIRSANPSYQNTAYSQCGEKYHTQAHDQDIPMVSSAPSSAYYSDLSVTAMPSGNDRAYEIVGLTCFAERGDLSGGGSSQRRIQRLAAINETAANTTVPSDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWY5</accession>
    <name>T1GWY5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008320-RA">
        <property type="protein sequence ID" value="MESCA008320-PA"/>
        <property type="gene ID" value="MESCA008320"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309185_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008320-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11581" checksum="581821E8DF1D478E" modified="2013-10-16" version="1">MSLEVLLSLSPMDFVMESIAAKSVLRLQGEVKEIFQCSLMDQRHWKEYQADAIVDPKSLDVETEIRRKQGWALSVLRNLRDYDMKLVFTSKHGLRPMGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX35</accession>
    <name>T1GX35_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377008">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX35"/>
    <dbReference type="STRING" id="36166.T1GX35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008373-RA">
        <property type="protein sequence ID" value="MESCA008373-PA"/>
        <property type="gene ID" value="MESCA008373"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932808_0_0_1"/>
    <dbReference type="OMA" id="FRHANGH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040154">
        <property type="entry name" value="Biotinidase/VNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609:SF14">
        <property type="entry name" value="BIOTINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609">
        <property type="entry name" value="BIOTINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008373-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14597" checksum="45D9BB0404DD1DEA" modified="2013-10-16" version="1">QVQSTFQKTLPSDPYYKAGVVEFAPILAHSTNSSIVEQLEGIRTIIQDPNAGNLDILVFPEGALSSEGLTYVPDPHEQVIPCEELDYDYSLSEISCYARSIQAYIAVNIHEKVQCLGDYQCPKKGYFEFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX37</accession>
    <name>T1GX37_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX37"/>
    <dbReference type="STRING" id="36166.T1GX37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008376-RA">
        <property type="protein sequence ID" value="MESCA008376-PA"/>
        <property type="gene ID" value="MESCA008376"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_17_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="178"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="234" mass="26574" checksum="B532645F5C8FA63F" modified="2013-10-16" version="1">MNDDIDDIILTILPIYWLSGLYGIIGSGVSGCTHLVTKEYFSPSTTVYLIRKYKVTKIFVVPPFISEIVNHPETTSESLSSLKVIKCSGCRLYPDIYTRIVDSNGNNLGVNELGQIVIFSKNPWKGYFNDPLLTSKAYKDGWYRTGDTGYFDDQGILYIVDRINDNIKMNGFHFAPSEVESVIYEMEGIREVCVLGLENRILVAAVVKEDYSNVDEDNILRHVAMKLEQFKHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX81</accession>
    <name>T1GX81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein kinase C</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02124328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX81"/>
    <dbReference type="STRING" id="36166.T1GX81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008423-RA">
        <property type="protein sequence ID" value="MESCA008423-PA"/>
        <property type="gene ID" value="MESCA008423"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212942_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20838">
        <property type="entry name" value="C1_nPKC_epsilon-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000003">
        <property type="entry name" value="Protein kinase C delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="16">
        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008423-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12158" checksum="CD5BB0A97DE15AA9" modified="2013-10-16" version="1">MKEEQQKQSEAAMQVGQRFNVNVPHRFVVHNYKRFTFCDHCGSLLYGLIKQGLQCEECGMNVHKRCQKNVSNTCGINTKQMAEILNQLGISPDKAPPRKSKSSPYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXI0</accession>
    <name>T1GXI0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02073840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXI0"/>
    <dbReference type="STRING" id="36166.T1GXI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008531-RA">
        <property type="protein sequence ID" value="MESCA008531-PA"/>
        <property type="gene ID" value="MESCA008531"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_5_3_1"/>
    <dbReference type="OMA" id="EFCHVIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008531-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8517" checksum="05A2F42044B5D7B6" modified="2013-10-16" version="1">ACEASLACTTCHVYVSDKYLEKLPEAEEKEEDLLDMAPFLKENSRLGCQIILKKELEGIEFELPKATRNFYNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY50</accession>
    <name>T1GY50_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Lysosomal dipeptide transporter MFSD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="28">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY50"/>
    <dbReference type="STRING" id="36166.T1GY50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008770-RA">
        <property type="protein sequence ID" value="MESCA008770-PA"/>
        <property type="gene ID" value="MESCA008770"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736002_0_0_1"/>
    <dbReference type="OMA" id="TTISAHM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="26">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008770-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="18090" checksum="20D350BCB0E5AB8F" modified="2025-10-08" version="2">MVICVAYYVAIFPFIALSKVFFEKRFGFEPDAANRITSTVYIISAVSSPFFGYIIDKTGRNVCWIFGAICTTISAHMLLAFTLLNPYIGMIIMGLSYSMLAASLWPLVALIIPEYQLGTAYGICQSVQNLGLATMAIAAGSIVDASNGDYTWLELFFICWLTGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY97</accession>
    <name>T1GY97_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008820-RA">
        <property type="protein sequence ID" value="MESCA008820-PA"/>
        <property type="gene ID" value="MESCA008820"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309200_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008820-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11611" checksum="2A06B7CDF5A66503" modified="2013-10-16" version="1">MIELLLKKTFSIKEVSKRKPEQSKKRIRTKEGRLTPLTRAEVDKAIERLENTTDGIHVELFKAARSGFSNEFHQILLNIWNTSIICPIHKKGDKSDCENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYA5</accession>
    <name>T1GYA5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">guanylate cyclase</fullName>
            <ecNumber evidence="3">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02188502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYA5"/>
    <dbReference type="STRING" id="36166.T1GYA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008828-RA">
        <property type="protein sequence ID" value="MESCA008828-PA"/>
        <property type="gene ID" value="MESCA008828"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001072_11_2_1"/>
    <dbReference type="OMA" id="LYHENIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000801">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000030">
        <property type="entry name" value="Si:ch211-215j19.12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF462">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="34"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="13">
        <location>
            <begin position="319"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="440" mass="48194" checksum="FF2B967A831C01B5" modified="2013-10-16" version="1">GSIASLKNLGLALPSKGNINGSGTAVAATTNTHNNNNSNAGSGTESLAKQTAYFMDMRARYNGDMVQLKEIQLNNSTELKSKAMDLLVLAHGLRHENINALIGWLSDHNRTAMVFDYCSRGSLQDVLIMDDIKLDWTFRLSLLTDLVRGMRYLHSSPIKCHGALTSRNCVVDARWVLKITDYALPSFYEAQGITPSQKSAKELLWTAPEILRNSKAFRCGTQAGDVYSFAIIMQEVVVRGEPYCMLSSSPEEIIAKLKKPPPLIRPSVSKGAAPPEAINIMRQYDDHSNEIPHLGGGHSVAEKLKLGLAVDPEEFSEVTIYFSDIVGFTTIAAHCTAVQVIDLLNDLYTIFDATINAYNVYKVETIGDAYMVVSGLPVRIPDHAEQIATMALDLLHQSGRFKVKYLPGVPLQLRIGMHTGPCCAGVVGLTMPRYCLFGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYE9</accession>
    <name>T1GYE9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYE9"/>
    <dbReference type="STRING" id="36166.T1GYE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008874-RA">
        <property type="protein sequence ID" value="MESCA008874-PA"/>
        <property type="gene ID" value="MESCA008874"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2339508_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008874-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11274" checksum="2A14EFD6898AF51B" modified="2013-10-16" version="1">MLIFEQSSKTTSTSVGNSSQKTDLNECESNIPRTVATQANQREIRHLKMFYILISLMAAFIVLRLPAWIFLIMRMYGDYTQPVDWILHFVFGILTLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYP0</accession>
    <name>T1GYP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYP0"/>
    <dbReference type="STRING" id="36166.T1GYP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008976-RA">
        <property type="protein sequence ID" value="MESCA008976-PA"/>
        <property type="gene ID" value="MESCA008976"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269681_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF63">
        <property type="entry name" value="STRAIGHTJACKET, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="160"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008976-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="24769" checksum="157F07E62BD506DF" modified="2013-10-16" version="1">MNNAQEIAKDHHNEKPQTNLSYYNAKEMLEPGETPPPPLTFDIDFAGIPPLNITPMTIVLTPTPEFFDEPINTSVSSVHVPTNVFDRAPEVIQAIEWSEKLDAIFKNNYKEDPTLSWQFFGSAFGFMRQYPSSKWKQDPVDLYDCRLRSWYMEAATSPKDIIVLLDGSGSMHGQRLDIARHIVYTILDTLGTNDFVNIFTFGSEIKAVVDCFNETLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYP4</accession>
    <name>T1GYP4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Identified in the spliceosome C complex. Interacts with SNW1/SKIP. Interacts with CDC40/PRP17; this interaction leads to CDC40 isomerization. Interacts with RBM22.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02189998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYP4"/>
    <dbReference type="STRING" id="36166.T1GYP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008980-RA">
        <property type="protein sequence ID" value="MESCA008980-PA"/>
        <property type="gene ID" value="MESCA008980"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_16_3_1"/>
    <dbReference type="OMA" id="ELYNDHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000008">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008980-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="19180" checksum="AD71FB6EC4BE8CD8" modified="2013-10-16" version="1">MLATSSLSSSGIPDKSWQPPTVTLETSMGEITIELYWKHAPKTCRNFAELVRRGYYNNTIFHRIIRDFMIQGGDPTGTGRGGASIYGPSFEDEIHSELKHSGAGILSMANSGPDSNGSQFFITLAPTQWLDNKHAIFGRIYSGMQVVKRIGMVETDGNDKPVDNVSIIRGRVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZR9</accession>
    <name>T1GZR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZR9"/>
    <dbReference type="STRING" id="36166.T1GZR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009374-RA">
        <property type="protein sequence ID" value="MESCA009374-PA"/>
        <property type="gene ID" value="MESCA009374"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161241_0_0_1"/>
    <dbReference type="OMA" id="ANGMYIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CLUMA_CG020620, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009374-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="11299" checksum="223E1BA8A643094D" modified="2013-10-16" version="1">MLTHRNKKPYECKAEGCGKSYCDARSLRRHTENHHQGLVSGAGTGTGSSTTNSNTATISSAISTAVITAKTPNNLSLMSSTGAAPIAASSTGPPTGLSLSPATANGMYILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZW2</accession>
    <name>T1GZW2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009420-RA">
        <property type="protein sequence ID" value="MESCA009420-PA"/>
        <property type="gene ID" value="MESCA009420"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1890971_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577270" evidence="2">
        <location>
            <begin position="20"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009420-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="14884" checksum="DC9EAABAC7E13004" modified="2013-10-16" version="1">MMYFLLVLIAALLLPSVEPILVSANSVPLENQSPSSDQYLESLEKSVAGIFQKVAYGTTSTTKRSIPDNYVPTLTTVATLPLTTERFTQSENANQKIRNFGIDRDHALPTSAPNADILKSNSNPSYPNPNRHHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZX3</accession>
    <name>T1GZX3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZX3"/>
    <dbReference type="STRING" id="36166.T1GZX3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009433-RA">
        <property type="protein sequence ID" value="MESCA009433-PA"/>
        <property type="gene ID" value="MESCA009433"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856324_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009433-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7556" checksum="CBF066FD3672EF82" modified="2013-10-16" version="1">MVYNNTYCILALWGLYKALTATSPWQRRLCFALPFTMRSILTLLRTMSLVPSTRESLTHIYLQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0B2</accession>
    <name>T1H0B2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0B2"/>
    <dbReference type="STRING" id="36166.T1H0B2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009586-RA">
        <property type="protein sequence ID" value="MESCA009586-PA"/>
        <property type="gene ID" value="MESCA009586"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286126_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000046">
        <property type="entry name" value="Putative B-cell lymphoma/leukemia 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001175">
        <property type="entry name" value="Zinc finger, C2H2 type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051497">
        <property type="entry name" value="Dev/Hematopoietic_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056438">
        <property type="entry name" value="Znf-C2H2_CTCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993">
        <property type="entry name" value="B-CELL LYMPHOMA/LEUKEMIA 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993:SF6">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23611">
        <property type="entry name" value="zf-C2H2_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="185"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009586-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="23737" checksum="9C1A050A48E08650" modified="2013-10-16" version="1">AAGHQQNQVPLFNPFENPFDVKRASSGHGGPNEWWNLSALHNNESFLDNLKMKHLPGSNSIMQNSMPVKKESRRNDTCEYCGKVFKNCSNLTVHRRSHTGEKPYKCELCSYACAQSSKLTRHMKTHGRSGKDVYRCRFCNMPFSVPSTLEKHMRKCVVNQGKVPANGAPSAGPLSALSAHLPQMPMISSSGSTGSLMDEDSSHHHQGISIKNEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0K1</accession>
    <name>T1H0K1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0K1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009681-RA">
        <property type="protein sequence ID" value="MESCA009681-PA"/>
        <property type="gene ID" value="MESCA009681"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760685_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009681-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7834" checksum="14C40A6832F9801E" modified="2013-10-16" version="1">MKLEEYQHRLRPPSSTNTTSRIENSILVARKGSSAAGPLVAIKGETERPPICAMFTKTSENPIFTKRLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0U2</accession>
    <name>T1H0U2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SOS1/NGEF-like PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0U2"/>
    <dbReference type="STRING" id="36166.T1H0U2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009783-RA">
        <property type="protein sequence ID" value="MESCA009783-PA"/>
        <property type="gene ID" value="MESCA009783"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087570_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF106">
        <property type="entry name" value="TRIO, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOS1/NGEF-like PH" evidence="2">
        <location>
            <begin position="56"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009783-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13506" checksum="8FBEA47564A73954" modified="2013-10-16" version="1">MTVEACRIRAPNAAKADPNSEEIENLKEAYKIVKAIVKTINDMMELQKLENFPEIISNQGKLVLHGNLSCSEEPSDQPQKFREYKVFLCEQSIIFADIIPRKNPYSDDTFIYRVILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2J5</accession>
    <name>T1H2J5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2J5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010443-RA">
        <property type="protein sequence ID" value="MESCA010443-PA"/>
        <property type="gene ID" value="MESCA010443"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3377603_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010443-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="34" mass="4121" checksum="553A9D9AFCF83F89" modified="2013-10-16" version="1">MNSQNSSKSPFFTLVLKLFFFSYFFVFIYILSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X1</accession>
    <name>T1H2X1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Dynein heavy chain ATP-binding dynein motor region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02192334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="MESCA010577-PA"/>
        <property type="gene ID" value="MESCA010577"/>
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        <property type="term" value="C:dynein complex"/>
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        <property type="term" value="C:microtubule"/>
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    </dbReference>
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        <property type="entry name" value="AAA_9"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MT"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="13">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="14">
        <location>
            <begin position="90"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010577-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26440" checksum="913AFFC475ECBE1F" modified="2013-10-16" version="1">IAADKLIGGLTSERTRWAEDLQSFRREQVELVGLCLLCASFLAYTAAFSWEFRKTMVSEDWLNDIIERNIPMTVPFKLDKSLSTDVEVSTWSSEGLPPDELSVQNGILTVRASRFPLCIDPQQQALHWIRKRESKNNLKILSFNDADFLKQLEMAIMYGLPVLFQDVDDYIDPVIDDVLQKNIRLQTGRKFVILGDKEVDYDPNFRMYLTTKFSNPKFDPLYMRKQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H347</accession>
    <name>T1H347_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H347"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010661-RA">
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        <property type="gene ID" value="MESCA010661"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10918" checksum="ECF9896AC04F87FB" modified="2013-10-16" version="1">MYLSRATYLHHQSCLGRQIYPMLEFHLHQIVGGGGGGGGGGGGEKLNIKTMTTMKKLKMMKDLNMNNLMKMKFQHWINLFTKTTLMMKIIMKNKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3T2</accession>
    <name>T1H3T2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071450">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA010919-RA">
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        <property type="gene ID" value="MESCA010919"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577964" description="WAP domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010919-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12119" checksum="474251C11150259E" modified="2013-10-16" version="1">MVKLTVLVAFIVMCGIVAFASECPSYSKHTNCSPKCVNDNECSHLGGKCCPNLCNARSCIQANQLGGGGGDKYGNNNKYNPGGGAGTYCGNTKCNSYEKCDFDKSTKRQKCVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3W3</accession>
    <name>T1H3W3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3W3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010958-RA">
        <property type="protein sequence ID" value="MESCA010958-PA"/>
        <property type="gene ID" value="MESCA010958"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3242719_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010958-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="43" mass="4742" checksum="77EC2A85906D33DD" modified="2013-10-16" version="1">MSILLIEAADLGDSGKYSCYPSNSEITSIRVHVLKARIYKGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4P3</accession>
    <name>T1H4P3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thyroglobulin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H4P3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011259-RA">
        <property type="protein sequence ID" value="MESCA011259-PA"/>
        <property type="gene ID" value="MESCA011259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639623_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thyroglobulin type-1" evidence="3">
        <location>
            <begin position="10"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18745" checksum="E513B3D85042EEAC" modified="2013-10-16" version="1">TGYANDRLNHSSCYFIYHINQSQYPILPQCELDETYKAVQCFGDKVTGGGRCFCNSETGERIYGWAWRKEAENMTCSCSRQMYKANGSLTLHCDEMGNYEPLQXXXXXXXXXXXXXXXXXXXXXXXXXAHKWCLLVCRQQVWRNFRRNKVSATINVEHITML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5K6</accession>
    <name>T1H5K6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Oligosaccharyltransferase complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Specific component of the STT3A-containing form of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSTC family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02092483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5K6"/>
    <dbReference type="STRING" id="36166.T1H5K6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011587-RA">
        <property type="protein sequence ID" value="MESCA011587-PA"/>
        <property type="gene ID" value="MESCA011587"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729573_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042416">
        <property type="entry name" value="OSTC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160:SF4">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011587-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8471" checksum="627DB8720337DE41" modified="2013-10-16" version="1">MSVVSWRHSPQPPRKCALGPLLFFDMFESLYQLPFYLIEPPNLRIRKPLWMQKPPSMIVFSTILLSYFLVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Z5</accession>
    <name>T1H5Z5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H5Z5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012128-RA">
        <property type="protein sequence ID" value="MESCA012128-PA"/>
        <property type="gene ID" value="MESCA012128"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168386_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="5">
        <location>
            <begin position="120"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="5">
        <location>
            <begin position="194"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012128-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="238" mass="27605" checksum="9530C3CA06021F31" modified="2013-10-16" version="1">RFKEWYWGLVFAAAWFVIYFVVVYPSFYAFPRTILISEEHNHIGEFIGGRAEKFHIELSSIGVKSLGSPGHHSTTQFLLGKLXISENSRNDLYDVEIDYQKXWKNELGIKIFYSYKDIPNIVMRVAPRNESLSSVLINSHYDTVPGAGDAGIMIAIMVEVFRVIVSDQNPLNHPVIFFXMELKKLDWSFFRIIIFHDTDFRIFRDFGQVPGLDLAHTYNGYVYHTKYDRISIIPPGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H639</accession>
    <name>T1H639_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H639"/>
    <dbReference type="STRING" id="36166.T1H639"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012172-RA">
        <property type="protein sequence ID" value="MESCA012172-PA"/>
        <property type="gene ID" value="MESCA012172"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128416_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040126">
        <property type="entry name" value="STOX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437:SF0">
        <property type="entry name" value="FI21431P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437">
        <property type="entry name" value="WINGED HELIX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8959" checksum="D822DC18F5FEAF46" modified="2013-10-16" version="1">MGTNSPGGPQRCRLILQRCLAIQLNKPGHTPEDLWMYDSGYMIFQNFLAANAQCWWNVPLTSATRVLKYTGHIAPGILLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H690</accession>
    <name>T1H690_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H690"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012223-RA">
        <property type="protein sequence ID" value="MESCA012223-PA"/>
        <property type="gene ID" value="MESCA012223"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1242807_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF145">
        <property type="entry name" value="FI17609P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="1">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012223-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="24598" checksum="82429662C1F81BF8" modified="2013-10-16" version="1">MIYEISKRCYYGDKSLFCGGGDDLPYVCCPSSPLEKNQLCGKSLVQGHFYKGLGTYPFIVRIAFKHVNTGSFAYPCSGSIISRRVILTAAHCALAKAEGHRLSSVRVGEFDIASDPDCASTGFCAPRSINHAISHVIVHPDYKQGHYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMSTSSKRSSEMVYLDVPLTTWELCLRNYGQTGALESPNSVEGQWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6W3</accession>
    <name>T1H6W3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homogentisate 1,2-dioxygenase</fullName>
            <ecNumber evidence="5">1.13.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Homogentisate oxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Homogentisic acid oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Homogentisicase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>homogentisate + O2 = 4-maleylacetoacetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15449"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:17105"/>
            <dbReference type="EC" id="1.13.11.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.5" evidence="5"/>
    <dbReference type="STRING" id="36166.T1H6W3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012445-RA">
        <property type="protein sequence ID" value="MESCA012445-PA"/>
        <property type="gene ID" value="MESCA012445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027174_0_0_1"/>
    <dbReference type="OMA" id="MLPHGPD"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00339"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07000">
        <property type="entry name" value="cupin_HGO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000026">
        <property type="entry name" value="Homogentisate 1,2-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01015">
        <property type="entry name" value="hmgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF0">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase N-terminal" evidence="20">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase C-terminal" evidence="19">
        <location>
            <begin position="271"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF20510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="425" mass="48205" checksum="3EDEA50685DD4F48" modified="2013-10-16" version="1">YQSGFGSHFETEDPRCPNALPVGQNSPQKCAYGLYAEQLSGTAFTAPRTENSRTWLYRIRPSVVHEPFQKLNVSKHSNWNEQPPNPNQMRWKPFDLPSSSEKVTFAEGLHTVCGVDTRSRHGLAIHVFMCNVSMTNSAFYNADGDFLIXXXXGILDITTEFGKIKVSPNEICVIQQGMRFSVNVEGPTRGYILEVYDNHFKLPDLGPIGANGLANPRDFLTPVAWFEDREVEEFKIISKYQGSLFSAKQKHSPFDVVAWHGNYVPYKYDLSKFMVINSVSFDHCDPSIFTVLTCQSSRPGTAIADFVIFPPRWSVQENTFRPPYYHRNCMSEFMGLIGKYEAKEEGFIPGGGSLHSIMTPHGPDRQCFDGASNAKLNPERIAEGTQAFMFESSLSMAVTKWGEETCQKLDSKYYQCWQTLKKYFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7A8</accession>
    <name>T1H7A8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Potassium channel voltage dependent Kv4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H7A8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012646-RA">
        <property type="protein sequence ID" value="MESCA012646-PA"/>
        <property type="gene ID" value="MESCA012646"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654847_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005250">
        <property type="term" value="F:A-type (transient outward) potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003975">
        <property type="entry name" value="K_chnl_volt-dep_Kv4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024587">
        <property type="entry name" value="K_chnl_volt-dep_Kv4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF105">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11879">
        <property type="entry name" value="DUF3399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01497">
        <property type="entry name" value="SHALCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent Kv4 C-terminal" evidence="9">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012646-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="420" mass="47179" checksum="6C32233D5EC62B92" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIQIIPGDCCYEDYRDRKRENAERLMDDKLSENGDQNLQQLTNIRQKMWRAFENPHTSTSALVFYYVTGFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYGDMVPETIAGKIVGGVCSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRKAQRKARLARIRIAKASSGAAFVSKKKAAEARLAAQESGIELDDNYPEDIFELQHHHLLRCLERTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7I5</accession>
    <name>T1H7I5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7I5"/>
    <dbReference type="STRING" id="36166.T1H7I5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012722-RA">
        <property type="protein sequence ID" value="MESCA012722-PA"/>
        <property type="gene ID" value="MESCA012722"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018687_5_0_1"/>
    <dbReference type="OMA" id="CLFVREF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000004">
        <property type="entry name" value="TBC1 domain family, member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="42"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012722-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="339" mass="40302" checksum="EF2DEB0D71205BEB" modified="2013-10-16" version="1">YPGRPQLQKFSNAQDGFEYDSKIEKINMFLNQQLLDLVELKKVSWSGIPKKWRLLSKYLPSSSERRDTTLIHKRKSYHELRLQYFKLDGYDESQQDTYRQIHIDVPRMNPNISLFQQKSVQQMFERILFIWAIRHPASGYVQGINDLVTPFFIVFLQEILQDTNIEEISTLEETIRDSIEADSFWCLSKFLDSIQDNYVFAQLGIQEKVNQLKELIQRIDGSLHKHLQNHGIDYLQFSFRWMNNLLTRELPLHCTIRLWDTYLAESDDFAVFHLYVCAAFLLSWREQLLQQNDFQGLMLLLQNLPTHSWTDRQINVLVAEAFRLKFTYADTPKHLEIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7J2</accession>
    <name>T1H7J2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleolar complex protein 2 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7J2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012729-RA">
        <property type="protein sequence ID" value="MESCA012729-PA"/>
        <property type="gene ID" value="MESCA012729"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392136_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="634"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="637"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="689"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012729-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="79912" checksum="902F52805C8F0102" modified="2013-10-16" version="1">PKKSKKNANSMKKKKLTNKKKNQHQKELEGLKDIDPEFYNFLKQNDKKLLDFDMLDKDDDDDDDEEAKGKVDTLNNHTNDEEDEDDTKFHKPSEKMEVASDEEDEDEDEEESEIVITLKVLRKWQSELTKASPSIGILRKVTLAFNSALASISGDEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEYLAHESASLAFPDLVVTTVTALKAYLKVCKNSNHSRKLKQIMDKIIDNSKFIEKRKKVTFGLKDTSLIQSWETQVRNKGTPLNIYFKSWVKTHEIKKRRQAANTDDINDYDIPYEEVWVKNKELLWDDGKVVLFPSDDEDEDDDIPMDSDEDDLGEEEEEVQPKKSKKSKKQAPVPEEEEEDEADANPDAHVDIVKDLDLDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA13</accession>
    <name>T1GA13_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA13"/>
    <dbReference type="STRING" id="36166.T1GA13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000056-RA">
        <property type="protein sequence ID" value="MESCA000056-PA"/>
        <property type="gene ID" value="MESCA000056"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888136_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000056-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15060" checksum="F6DD9F4536DE6F39" modified="2013-10-16" version="1">MTKLAAKELNIERLDVSESLAMEMFADNPYKKQQIPDIANSGENSNVTLYRLGNHIDISRGPMVQNTRFLGKCTISSVHEVGKDEKLGIYRVQGVALPAGTILNHFAYSILEDRSKKLNPARLPTEPFEEQALMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA34</accession>
    <name>T1GA34_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA34"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000079-RA">
        <property type="protein sequence ID" value="MESCA000079-PA"/>
        <property type="gene ID" value="MESCA000079"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3371109_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054721">
        <property type="entry name" value="GEO12453p1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22861">
        <property type="entry name" value="GEO12453p1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577148" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000079-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="35" mass="3471" checksum="5627B1D9CCE6CF85" modified="2013-10-16" version="1">MFKLTLAIFAVIACAAAKPGLVAPLAYTAPVVAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAB2</accession>
    <name>T1GAB2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02100421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02100422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000166-RA">
        <property type="protein sequence ID" value="MESCA000166-PA"/>
        <property type="gene ID" value="MESCA000166"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530046_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000166-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9642" checksum="4EC0AFBDE5FEC0C4" modified="2013-10-16" version="1">MDTLWKQYVLFGSSTIEDIRRGYCYLMVHNVSGCIPFLLRIRGILLSSMARCAWVGNPQSYTESVGLKKTDAHRNRNNHPHGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAH0</accession>
    <name>T1GAH0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02031462">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAH0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000227-RA">
        <property type="protein sequence ID" value="MESCA000227-PA"/>
        <property type="gene ID" value="MESCA000227"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673946_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000227-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8864" checksum="0B587C79B3F4CAC5" modified="2013-10-16" version="1">MEVVNYFSNLRTVIYILFLILPRFNSLLSLLSLLTGNRLELRGLVNWYLGGIDRFRRISLISVWELSLLFNEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAQ2</accession>
    <name>T1GAQ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbon catabolite repressor protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic deadenylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAQ2"/>
    <dbReference type="STRING" id="36166.T1GAQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000311-RA">
        <property type="protein sequence ID" value="MESCA000311-PA"/>
        <property type="gene ID" value="MESCA000311"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016428_4_2_1"/>
    <dbReference type="OMA" id="PHYYARA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000343">
        <property type="entry name" value="CCR4-NOT transcription complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000311-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="475" mass="54683" checksum="C0C3EAC745A2E0B6" modified="2013-10-16" version="1">MTRNKDKYNENHNHRRQQIFMSAEDLAAGKKPQWSGLEITGYVRNISPQLWAFEHLTTLYLNENMLLRLSSDIGMLTNLRVLDVSSNKLRSLPAELGELVNLRELLLNNNFLRALPHEIGKLFHLQVLGLHGNPLQKEIINIYNEPNGTQKLLTYLLDNLTITAPPPPQRPWIPLARPNKQRPACIFTVMCYNVLCDKYATRQMYGYCPSWALEWDYRKKAIIDEIRHYSADIISLQFSLIKEHLIEFNQLAMANAEGSDDMLNRVMPKDNIGLAALLKVKESAWEPMNPEVTQISQPILVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEANCSFRPGHKFDSNILSSGRVAMDHMDFKERLYKSCLQRLLSNETNEFTHGFKLASAYSEDIMPYTNYTHDFKGIIDYIFYTKTGMVPLGLLGPISQDWLRENKVFGCPHPHIPSDHFPLLVELELTHTASQQPPNGLINRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBD9</accession>
    <name>T1GBD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000571-RA">
        <property type="protein sequence ID" value="MESCA000571-PA"/>
        <property type="gene ID" value="MESCA000571"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388721_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000571-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10570" checksum="F45CEB8998F996FB" modified="2013-10-16" version="1">MVSLSLFQEVPTRAFKALFLEITECHTYAERFAIVWVLSNVTTKILGFLTVETCSFSNGEPTIVAKHEGANRMGQRRGLSESDILKINRLYGCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBZ2</accession>
    <name>T1GBZ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000785-RA">
        <property type="protein sequence ID" value="MESCA000785-PA"/>
        <property type="gene ID" value="MESCA000785"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485914_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000785-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10203" checksum="923BF5A6078AA36F" modified="2013-10-16" version="1">MMKREVGGNRRRRAETTLPQSVEQRQHPPFIIISYLNTLHIRQKTSSESNINLNATIRIQQVIYRKLSPVWEGELGFSCEYSLLLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC51</accession>
    <name>T1GC51_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC51"/>
    <dbReference type="STRING" id="36166.T1GC51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000849-RA">
        <property type="protein sequence ID" value="MESCA000849-PA"/>
        <property type="gene ID" value="MESCA000849"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1462909_0_0_1"/>
    <dbReference type="OMA" id="FVILTIE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000849-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="21126" checksum="E2C3ED2F7886D95B" modified="2013-10-16" version="1">MGAGEVKCYGTSFFLKKKFGSGYILTCVKTDMCDSSNVSRLLRKYIPNIQIQQEIGAELSYQLPDEYTSKFEDMLSDLEKHSSDLFLNGYGISNSSLEDVFMKMGAEVHDDTKENSRVISNLFGPEVAPLLKGYTLEWNQWKAMLLKKYIYSVKNWIIVAIQFIVPLIFVILTIEIEVSQSTYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC78</accession>
    <name>T1GC78_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4776 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC78"/>
    <dbReference type="STRING" id="36166.T1GC78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000879-RA">
        <property type="protein sequence ID" value="MESCA000879-PA"/>
        <property type="gene ID" value="MESCA000879"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1457935_0_0_1"/>
    <dbReference type="OMA" id="ICHEESL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031949">
        <property type="entry name" value="DUF4776"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39079">
        <property type="entry name" value="FI08034P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39079:SF1">
        <property type="entry name" value="GH11706P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16003">
        <property type="entry name" value="DUF4776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4776" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000879-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="21628" checksum="CE6AE29752727154" modified="2013-10-16" version="1">HKKKKKCKPEEPCCDDLVQKPTLHILKKKGEYIITLRPLKDPTYLKQCENPYLDMKPLQFKIAKNPIVVQKREVKKKLMDKGFKKCVCHKPVSSCCCRPYIEKKQLEYDLSKICCEMGINSLKDDLVYSDVTDSDSDLDIEFTPPAGLIKPSLRKKPDMAHMETQYLESDWNARPIFTPPINKRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCC3</accession>
    <name>T1GCC3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02048563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02048564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCC3"/>
    <dbReference type="STRING" id="36166.T1GCC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000933-RA">
        <property type="protein sequence ID" value="MESCA000933-PA"/>
        <property type="gene ID" value="MESCA000933"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1046925_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000933-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="266" mass="29329" checksum="989BBF972971744C" modified="2013-10-16" version="1">MKELDIARFDLSGTQSKLSSIEDNSFGSNDMGVGIDSPRWPIGSQNDSLRAEIEAQTAAHKAQIGTLENRAHETWLAARQSERKYEEARLEGANLRRKLTQLSQNGVSQNTTDGIQSNTSINSAPSPVHMEAPGSPILQLPPPPPFLPPSFMGLPPPPMMGPGGAPPPFPPMFLPPGEIRPPPLGRLMSPPPHHHSRYIDDGNSILDEIMTMNTMTTMRIVDPEYIPEMEDLPQIVDMITIRGMTRKLILVHHQVPHRSPGVKMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCI9</accession>
    <name>T1GCI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gem-associated protein 5 TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCI9"/>
    <dbReference type="STRING" id="36166.T1GCI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001003-RA">
        <property type="protein sequence ID" value="MESCA001003-PA"/>
        <property type="gene ID" value="MESCA001003"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1808414_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052640">
        <property type="entry name" value="Gemin-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056421">
        <property type="entry name" value="TPR_GEMI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362:SF1">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23774">
        <property type="entry name" value="TPR_GEMI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gem-associated protein 5 TPR" evidence="1">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001003-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="16557" checksum="FAA56DC57680C8C5" modified="2013-10-16" version="1">MAVKGKTLTEWHVSMAPMISYDFWKKCCGAFAAQLIDQGYILQAASYLSCLHQTKEVIAILLKNTLFLEAWCYAKLHLEDGDHLFKDIIEKWTRTLEMNGNIEGAAYISALNNNFEKCRDLLRKRRRDEEGDKLLQLVEEKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCK3</accession>
    <name>T1GCK3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCK3"/>
    <dbReference type="STRING" id="36166.T1GCK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001018-RA">
        <property type="protein sequence ID" value="MESCA001018-PA"/>
        <property type="gene ID" value="MESCA001018"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256785_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007167">
        <property type="term" value="P:enzyme-linked receptor protein signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09933">
        <property type="entry name" value="SH2_Src_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051184">
        <property type="entry name" value="Tyrosine-phos_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969:SF5">
        <property type="entry name" value="CRK-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969">
        <property type="entry name" value="SH2-SH3 ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="3">
        <location>
            <begin position="21"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001018-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11998" checksum="66BAFF51AD3655BC" modified="2013-10-16" version="1">MEGLIPLNFVAEERSVNSEDWFFEKVLRKEADKLLLAEENPRGTFLVRPSEHNPNGFSLSVKDWEDGRGYHVKHYRIKPLDNGGYYIATNQTFPSLQALVMRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCK7</accession>
    <name>T1GCK7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCK7"/>
    <dbReference type="STRING" id="36166.T1GCK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001022-RA">
        <property type="protein sequence ID" value="MESCA001022-PA"/>
        <property type="gene ID" value="MESCA001022"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137535_0_0_1"/>
    <dbReference type="OMA" id="MAILRCR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000129">
        <property type="entry name" value="CLUMA_CG018772, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF41">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001022-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11215" checksum="BA0392FE2504043F" modified="2013-10-16" version="1">MEKPTEIQPFFENTTQRSAIAAVGRTAQFHCKVRNLGDRAVSWIRKRDLHILTIGIMTYTNDQRFQAMHLDDTDEWILKIVSVQPRDSGIYECQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD21</accession>
    <name>T1GD21_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin/FtsZ 2-layer sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02067201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD21"/>
    <dbReference type="STRING" id="36166.T1GD21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001200-RA">
        <property type="protein sequence ID" value="MESCA001200-PA"/>
        <property type="gene ID" value="MESCA001200"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1512327_0_0_1"/>
    <dbReference type="OMA" id="EMEECEF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001200-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20457" checksum="AF737F5E2B314CFB" modified="2013-10-16" version="1">MYCTVVEPYNFTTLEHSDFAFMVGNEAIYDICRLNLDIERPTFTNLNCLIGQIVSSITSSLRFDSTLIFDLTEFHTNLVPFPRIHFPLVTYAPVMVSNWFQGRCQLPTTNLIAKVWARLNPKFNLMNAKRDFVHWYVGEGMEEGEFSEVREDLAALEKDYEEVGKSSGDGEEEGAEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDF3</accession>
    <name>T1GDF3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02069709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001339-RA">
        <property type="protein sequence ID" value="MESCA001339-PA"/>
        <property type="gene ID" value="MESCA001339"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725080_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001339-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8157" checksum="5050BC8A75B77E1B" modified="2013-10-16" version="1">MTIVCDLIVNYLTAGTTTENFRKIRRFRKGDALVMLSANIYNGKIIFNKSNQILAYVDDIELIVRSSNNISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDG8</accession>
    <name>T1GDG8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001354-RA">
        <property type="protein sequence ID" value="MESCA001354-PA"/>
        <property type="gene ID" value="MESCA001354"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485922_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001354-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10244" checksum="210434EEB29D2E51" modified="2013-10-16" version="1">MYRKDRLKYVFFGGNICDESTTYKENTAISAMVLLFCIANTNNDKLMLMSTTSPKWIRVLDSHNKRRYVAFSLRKKGIENNLWFPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GER8</accession>
    <name>T1GER8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02021015">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GER8"/>
    <dbReference type="STRING" id="36166.T1GER8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001839-RA">
        <property type="protein sequence ID" value="MESCA001839-PA"/>
        <property type="gene ID" value="MESCA001839"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_3_1"/>
    <dbReference type="OMA" id="CIFRCGY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF270">
        <property type="entry name" value="ENTOMOGLYCEROPORIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001839-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26823" checksum="B406A0C0EC4FF4A5" modified="2013-10-16" version="1">MKSKDPRNSVMKAKVLDMISIFLAELIGTALLVFFGCMGCIDFNNGDSIQMTNSLFQISVVFGLVIMFIVQIFGCVSGAHLNPAVTIAAIVYKLLTPAMAVGYFVAQMGGAFIGYGLLQILVAKDVMANEHGVGMFIELIGTGTLIWVVCGVWDPRNAKGHDAVPLKLGFAITALCGCFGHWTGCSMNPARSFGPALWNMDFGQHWVYWVGPLLGGLLASLSYRHVFYRNQLRSMRREGWKRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF38</accession>
    <name>T1GF38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the globin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02037542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF38"/>
    <dbReference type="STRING" id="36166.T1GF38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001967-RA">
        <property type="protein sequence ID" value="MESCA001967-PA"/>
        <property type="gene ID" value="MESCA001967"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_13_1_1"/>
    <dbReference type="OMA" id="ALIESTW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47217">
        <property type="entry name" value="GLOBIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47217:SF1">
        <property type="entry name" value="GLOBIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="7">
        <location>
            <begin position="4"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001967-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17896" checksum="190A40D4E106469F" modified="2013-10-16" version="1">MDLDISEKEVLEIKRTWKIPMADPLTSGETMLLKLFERYPANQKKFQDLKDLPFKDLKDSPKFKFHSVRIMKAFDEAIQSLGTQNAGMVLHEIFEKVAVSHHKRGISKESHNQLKEVIIETLVGVCDLNDFQKGAWEKVMESIFNVIYSENWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFL0</accession>
    <name>T1GFL0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFL0"/>
    <dbReference type="STRING" id="36166.T1GFL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002154-RA">
        <property type="protein sequence ID" value="MESCA002154-PA"/>
        <property type="gene ID" value="MESCA002154"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023330_0_1_1"/>
    <dbReference type="OMA" id="MEGYQLR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19601">
        <property type="entry name" value="serpin42Da-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000030">
        <property type="entry name" value="Serpin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Serpin" evidence="9">
        <location>
            <begin position="11"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002154-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="378" mass="42956" checksum="B70F5D2E1E8482B4" modified="2013-10-16" version="1">MSNENSVKFSKELFETISLSTDPGKNIIISPLSVQTSLGLAFVGATGETAQEFIKVLNLDSDNKDEVAGAFEKILEECSSNVDIANKIYVNQNYNLKESYNEITKKCVQSDLAADKINKWVEEKTNNKIQNCISPDFIDDSTQLILVNAIYFKGNWEHPFDSEFTKKARFYIGKNESVNVDMMSKNTYFRYGDLEDLDAKVLELPYENSNLSMLIVLPNKRTGLRDVELKLKSKSIFEINEKMVEDKVHVYLPKFKVAYELELTETLKKMGMTNIFSNEAEFDDILEGSGNLKVSKVLHKAFIEVNERGTEASAFSGTGLCEYCLPIDFIVNHPFIYIIWDLKNILFSGKIIAMSLVNQTKSVLVHFPRLVLSMTQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFP0</accession>
    <name>T1GFP0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392339">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002187-RA">
        <property type="protein sequence ID" value="MESCA002187-PA"/>
        <property type="gene ID" value="MESCA002187"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815352_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002187-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7829" checksum="23D8ED5262426626" modified="2013-10-16" version="1">MTSSQNFIVNRTLNLQNALVHNQSKHLFPSPYNSFSDMESTLTSPCYLRHMPCFKHPGHKVFERLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH63</accession>
    <name>T1GH63_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02048064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002749-RA">
        <property type="protein sequence ID" value="MESCA002749-PA"/>
        <property type="gene ID" value="MESCA002749"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199956_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002749-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12576" checksum="FFA32FADB61AA4DC" modified="2013-10-16" version="1">MFLIMKVVSKSVRLVKLAYIVTGVMLSKEDVCTTVDSISLVPQVSPKDLLKPKNYFKLYGMSKEFIYNFYEFLKILWPYVPVETLKIYYKLPMAMLKVPKFIFDSVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHH1</accession>
    <name>T1GHH1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02177183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHH1"/>
    <dbReference type="STRING" id="36166.T1GHH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002872-RA">
        <property type="protein sequence ID" value="MESCA002872-PA"/>
        <property type="gene ID" value="MESCA002872"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_366128_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905">
        <property type="entry name" value="NINEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905:SF13">
        <property type="entry name" value="NON-CENTROSOMAL MICROTUBULE ARRAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="361"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="234"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="310"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="419"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="642"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002872-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="762" mass="87579" checksum="CADE8143B8C73668" modified="2013-10-16" version="1">MGYGSEIGTHYFTKENLAVVFQKVGIAEKIAGKVAEEVFEKTPANAINLKQFIALIHGPNSDIEFDFDLTNDLCYENDGNTNSLSTLVDESERDFLFKDNLSSKKLETIPVNSVVEMWERVGIPEPKQLLTALGFDIEEDCEINIKKISRMLDDSIQSHEIDYSETVLLKAALALPAVKYKSDANRRAALLAQEVDERHAHLESTSKEQIKCLEQRHAEAIKEIKAQFTNDKEITRLESQIKSYQDELSKLKDEITQLTADNTNLENEQVNFQKQITDLLEANIKLNNEIVDRETIPISGTPSGTETEEVLELFNKISVLQSENANLRDKNDELMSEVESLTVDLQRFKIKASKVVSEDEIDQSGGAVKRRGDSPSKTQIPEESPRTGKLRRCNSETELEGECVAVELQNPLLSPNSKVSQLESRCKELETSLDQLSKAYEDCEDYWQDKLSQERKMFEKERQIYDEEQQDNDRKFTELMEKVQEKDGLEQQYTDLEKEFEEFKEKSMVEFEEKCKEIEELRTEIDGLRLEINENKELFDDIAKSSTQHILNTSNSPASSPISYLMQQSTIQSPMKNRMDLEDPRQSRSLFNNGTGIFQRRRRQFNVILMASQHSCFETHSVASTHSVHKSLPGGTSNSNGIKEELKRLKFFEVQLKEHIQDLTIKRDSLVMELQQLQEAKPVLEKAYARAAHPSLIQRVNQLELRNRHLQNVIKQQQHYTENLLNQTWQQYTAEKNEAHSRLEAQAILIKSKLNVLQTLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI85</accession>
    <name>T1GI85_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003154-RA">
        <property type="protein sequence ID" value="MESCA003154-PA"/>
        <property type="gene ID" value="MESCA003154"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2747383_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003154-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7568" checksum="10C7464DB688656F" modified="2013-10-16" version="1">TSIEVQISVVLKEDKSLAVPISDIILDIDTGSESISVQFTNPWALAPMALNIKFTSSWGPTIKLVPVSAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIS2</accession>
    <name>T1GIS2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIS2"/>
    <dbReference type="STRING" id="36166.T1GIS2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003357-RA">
        <property type="protein sequence ID" value="MESCA003357-PA"/>
        <property type="gene ID" value="MESCA003357"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_521039_0_0_1"/>
    <dbReference type="OMA" id="FRHEIMN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21189">
        <property type="entry name" value="CH_PLEC-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000022">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000064">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="227"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="453"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003357-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="523" mass="61051" checksum="AC6DF7C80A6E8A89" modified="2013-10-16" version="1">MYKIDEEFRPRGRVFMKATQESSSSVPSSTSSTVERKSSLKKYSSTSGGQPLAKGPPITTNIDDRNIRFQHEVKHEKTIQAVIRDYPDARDMPYPPGTSTTDQSMVLQHSIQNGSGNIPVVSQDTQSYQAITRNKRVSTEVLGSSMESTKLSQRGEGGHRRITTHIVRKVTTLSRAEEQAHAQNLLPPQGRRNSVRTTEIGYMEESRAIEPKRPKISDIVVGKEDNVSAREALLRWARRSTARYPSVHVNDFTNSWRDGLAFSALVHRNRPDLLDWRQARSARPRERLETAFHIVEREYGVTRLLDPEDVDTNEPDEKSLITYISSLYDVFPEPPSIHPLFDVESQKRVHDYRELAQQFIYWCREKTAYMQERTFPPTLIEMKRLLEDLKRFRNEEMPPKQRDKNKLQQIYRELERYFESIGEVEVEPELRPEAVEKAWYKLQSAFADREQILQQEVDRLERLQRLADKLQREIKHCDLKITDLETRIIDEGRRMKGYTQLMLRILLRRWKLKSDTSKNPSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIU6</accession>
    <name>T1GIU6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIU6"/>
    <dbReference type="STRING" id="36166.T1GIU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003381-RA">
        <property type="protein sequence ID" value="MESCA003381-PA"/>
        <property type="gene ID" value="MESCA003381"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_281909_0_0_1"/>
    <dbReference type="OMA" id="KIFPCGE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390:SF159">
        <property type="entry name" value="GROWTH FACTOR INDEPENDENT 1 TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="380"/>
            <end position="408"/>
        </location>
    </feature>
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        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
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            <begin position="92"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="649"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="699"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="750"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="848"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="977"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1035"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="113"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="499"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="569"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="602"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="750"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="776"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="799"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="901"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="931"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="984"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1035"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1059"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003381-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1110" mass="123798" checksum="A00F1BF9D9BF3019" modified="2013-10-16" version="1">MEFIASGRPLLGAGGVCGSSDPNKHNDSPHSLAALELSAKNDESQASHQFDGESYIEELEEFEKAMVVETGNSAKNGLTYFNEDEYPMDIEDDYNQHQDTNTSLTTESSDDATNTTITNRTSNNNMMNTIVDNNSTNSITLNSIPDEEPKEEDETGSVDMGHAMSGAGADSVGVGRNTSGIGLSSVDPDGDGSSSLSQPPPAKKPKPTYSCLICPKSYRKRKSLIDHYKQHPGYCHDCGQPSGHSLEEIIAHNRSVHSKEFPFVCETCGESYSRRQQFHAHVEAHNKKEFKTYSCAECGLKFSQKKLHLQHLDETGHRVDGAICEICGTELQSKNALAQHIIREHKKDNFFECHICQNRFTLKANLERHVQLHTEVKRTYVCDQCGSSYFTYVALREHYSNAHTDASECKCTLCGKTFGSVKSLQRHLPSHSEERPHNFAIVLRFTFKWKTHLVRHKQTVHANLSKNNKGSVKKTERINEDSTGPDGLQHPQMLSTSASTSKPQNKKSNSNSKTKQLPPMHPQQQSQMQQQQQQKMIVNTPSPMPTSTIQQPPPPSNSQQMHMMQNHPTTVTGTSATSHISESHSNPIYNNQSFNTENLLGQQCNPQNQQQQSPSPQQQTPHTHPHPPPQQQQRRTPNAVDNSMLLRMQHHQQHQQQQHHHPQGFPPVSGGPETQFHHFDPNTGRYNHIQTHEQLLSQYQQQHPPTPQAHGQHGQQHYQSHLQHPSLIHNHPHQHAQLQQQIDTIQPSRLNPIHNKTSAPGSSSTDWNNLSFNSRTPTPASAAAAAAAHHHQPQPPHPQATSSTPTAATPGGATSAAAEPKESKLFHFNVNSNEFMGLPNQLVGVDVGAQHHHQHQQQQQQQQQQQAHHHQAVGSIKQQQQQHDINSDIMGFQNMWPPNHPQSAFTATQAEQHSLQQHHQLQHPHHHNPHHQQQQHQQQQQQQHHSQNYNNNIGSILTNLELIGNNNPGMEYNFDLVAPPPPLVAQHQHQQQQQQQPNSHPQAHPLSYLNYDYANPNHTLTDISDQKNNFQPYATPPIPTPPPSVASSTGTAVPYDNGTRGGGGGGSGGPGPVYQQTEDTKTILPPISDYMQHHHPASEVVTFTIPVKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIV6</accession>
    <name>T1GIV6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02122440">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02122441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003391-RA">
        <property type="protein sequence ID" value="MESCA003391-PA"/>
        <property type="gene ID" value="MESCA003391"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2336001_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003391-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11705" checksum="B80C187DD2486451" modified="2013-10-16" version="1">MEATKLYRQKRRRFEKRKSKTGSKLLNGDLSRVTPHEEDSLSFDNTEDIPHHLWKKFKLQSDDIPAEQWMCKVKITCKIQKQKKPFSDNQNKLLAKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJQ1</accession>
    <name>T1GJQ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02102267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003701-RA">
        <property type="protein sequence ID" value="MESCA003701-PA"/>
        <property type="gene ID" value="MESCA003701"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640958_0_0_1"/>
    <dbReference type="OMA" id="FANQHND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003701-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="9314" checksum="1E7406D9737B0F61" modified="2013-10-16" version="1">MAAQHETIKYLDTILKEEENRYDEYYNERRKYSISRSGRFRSKNKKRDALDTNLFANQHNDENSHQGHYENALYSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJX9</accession>
    <name>T1GJX9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LplA family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02064359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJX9"/>
    <dbReference type="STRING" id="36166.T1GJX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003789-RA">
        <property type="protein sequence ID" value="MESCA003789-PA"/>
        <property type="gene ID" value="MESCA003789"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_4_1_1"/>
    <dbReference type="OMA" id="RYQNWDW"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00595"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017118">
        <property type="term" value="F:lipoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="LIPOATE-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561:SF3">
        <property type="entry name" value="LIPOYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="3">
        <location>
            <begin position="77"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="391" mass="43642" checksum="697687BBC9733D78" modified="2013-10-16" version="1">MSGAFKLIQCSGRTTLFRSTAALRAFSSTSSSSSSTTTATPSEGHKSDIKKSVFISQSHDIFTNLALEDWIYKNFDFTNHHVLMLWANDPCVVIGRHQNPFTETNVSKLVESGITLARRNSGGGAVTMIVYNLNIITRALFREWNIKADLNERDDIVIGGKKVMEKRRRGEIRWHQLIRGLKFQISGTAAKLGHNAYHHCTLLVNANKLHLGESLAKEELKYVSKATASVRSQIKNLCDVNKTVNVPQLLSAVGYEFLRTSATELVDGGHSQTMKQQGFQLINPTEKWFPGITEIRDMYASWDWCLGKTPRFEVEKEIQIKADNNDLKMTLKVEVDKGLMTNIELVLPTGEKAPIVTGLQNKPYDEASLHGIINALKLVPTENVKEAINGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKC0</accession>
    <name>T1GKC0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003941-RA">
        <property type="protein sequence ID" value="MESCA003941-PA"/>
        <property type="gene ID" value="MESCA003941"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3282411_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003941-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="4886" checksum="85511E50D9372512" modified="2013-10-16" version="1">MVRIVKKDQEKIELLASLNRVNEEFEGTDDNKKNLWKIIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKQ5</accession>
    <name>T1GKQ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02011629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKQ5"/>
    <dbReference type="STRING" id="36166.T1GKQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004082-RA">
        <property type="protein sequence ID" value="MESCA004082-PA"/>
        <property type="gene ID" value="MESCA004082"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="OMA" id="HCADGAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF134">
        <property type="entry name" value="AT07769P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588382" description="Peptidase S1 domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="35"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="250" mass="26432" checksum="EA81E9D3ACE421AA" modified="2013-10-16" version="1">MKTLIIASFFIAVSSAAFLSSKTVKFKDLLPEQRIVGGELASSGQFPYQAALYITGPDGNAWFCGGSIISDQWILTAAHCPQGSVKVEVTLGSTNFQVLEKGGTHDIALIKLPSKIKFTSHVSPIKLAARADPVHTYDGDVAIASGWGKTSDAALTAVDDLHFAPLTVISNEECSETFNADDSTLCTSTKGKISVCSGDSGGPLVSTTTNEQIGIASFVSAKGCEKEEPAGFVRVTFYLDWIKNETGLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM02</accession>
    <name>T1GM02_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004570-RA">
        <property type="protein sequence ID" value="MESCA004570-PA"/>
        <property type="gene ID" value="MESCA004570"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906672_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004570-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7071" checksum="6FA47F9D72E113D5" modified="2013-10-16" version="1">MSRQIILGILEIATGKIIIMLIKYIINFKTCLDMLTKDKNFTKKLQDPRTELLSLCVTKVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM23</accession>
    <name>T1GM23_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM23"/>
    <dbReference type="STRING" id="36166.T1GM23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004591-RA">
        <property type="protein sequence ID" value="MESCA004591-PA"/>
        <property type="gene ID" value="MESCA004591"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_9_1"/>
    <dbReference type="OMA" id="REQRNDY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF7">
        <property type="entry name" value="GTP-BINDING PROTEIN DI-RAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004591-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12678" checksum="AD477F99E462EC1B" modified="2013-10-16" version="1">MSDERLRLVVLGGSGVGKSSILKRFICKTYTDRYKATVEDLYTREYAIGEQTLKVDILDTSGDMEFPAMRRLSIANANGFILVYAATSAPSFACVKQCFEEIREQRNDYQKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM90</accession>
    <name>T1GM90_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. ATF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02046250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM90"/>
    <dbReference type="STRING" id="36166.T1GM90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004659-RA">
        <property type="protein sequence ID" value="MESCA004659-PA"/>
        <property type="gene ID" value="MESCA004659"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_538935_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051882">
        <property type="entry name" value="ATF_bZIP_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46164">
        <property type="entry name" value="ATF6, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46164:SF3">
        <property type="entry name" value="ATF6, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="185"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="506" mass="57609" checksum="512F6835AE15C31C" modified="2013-10-16" version="1">MDYPPPSSVFDDFPPCWDSIPTLDVQFEDLLTANPAEPSTFNNLDFNADDLLNFLDDQQAHFPNPPSPSTSWISNTSDYSSGSSTKDFPENSESKIPEKTDPPPIKKPKTIILPQKDFKALMAKIKNGTTDLKTELGSKRITIKKTVPASALPKIAPSEAPPKPAPVAPLPISLLESNMIIDEKVLKRQQRMMKNRESASLSRKRKKDEMSRLEEENIKLKQENSSLRSQLLTFAQVCKCKHFQISGFVTNLVKTEPEEVSWSGKNKPNLFSSVSKKNTAVLLAMVFMVSLNFGRFSLPNFSVQDDTQMSQSPPENFAGGRHLLWFANENTENVNENTLAEGPMNVNDLEKFEKDQSSKCGSLKSNQTENMRLAMELQKWIKVNNVHNISSIDESLEVLKLSMNFMESTHFKKMFKKSFRNRKYFGRHRNLFDNGSSSDNKNSYNYNQDNENTFMNVYKPKFSDEYSKLFEGIGRRNDTFYVLTFNADNIYLPASAYNNPIDLRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMZ0</accession>
    <name>T1GMZ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02065492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMZ0"/>
    <dbReference type="STRING" id="36166.T1GMZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004927-RA">
        <property type="protein sequence ID" value="MESCA004927-PA"/>
        <property type="gene ID" value="MESCA004927"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1789062_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000062">
        <property type="entry name" value="Neuronal PAS domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF36">
        <property type="entry name" value="PROTEIN SINGLE-MINDED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="2"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004927-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16808" checksum="58F0228DFEBB98D9" modified="2013-10-16" version="1">MALREKSKKAAKDRREKENIEFDELSKLLPLPCATKSQLDKASIIRLTTSYLKLRQTFPEGLGEAWGSTSHSSSKINGKHFLQSLEVAKMLECGKNSSSMQKPRKPHGWWVRPRILYEKKPQKQELKNKVTDKRRIDLKRTSLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNN6</accession>
    <name>T1GNN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNN6"/>
    <dbReference type="STRING" id="36166.T1GNN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005194-RA">
        <property type="protein sequence ID" value="MESCA005194-PA"/>
        <property type="gene ID" value="MESCA005194"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062828_3_2_1"/>
    <dbReference type="OMA" id="NYCERIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000173">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 15" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="14 15" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="16">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005194-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16254" checksum="AD231615B2608C0D" modified="2013-10-16" version="1">MSGRGKGGKVKAKAKSRSNRAGLQFPVGRIHRLLKKGNYAERVGGGAPVYLAAVMEYLAAEVLELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLSGVTLLKEVSCQTFKLFRTKSTMCSRTAGTLRVYWMYARSEVQTLTPTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP94</accession>
    <name>T1GP94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP94"/>
    <dbReference type="STRING" id="36166.T1GP94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005415-RA">
        <property type="protein sequence ID" value="MESCA005415-PA"/>
        <property type="gene ID" value="MESCA005415"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1121201_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF167">
        <property type="entry name" value="PROTEIN DIMMED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="118"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005415-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="248" mass="28043" checksum="ABCA4B1F4BF4B9D6" modified="2013-10-16" version="1">MESASETARLLWVEYGPSTTIYFSSIPTPTPSVEEIDLNLSSSSTSNRPCRKAAAANNKKVSDMTNVFDVEMTDSSSQSDDTSGGGGSSNGGMRTCKRNGLSNGRRKKGVLSAKDRNLRRLESNERERMRMHSLNDAFQSLREVIPHIKKERRLSKIETLTLAKNYITALTDVITKKRGDELSTNLSLTNPGHHQQIQPQEQQPQHHQQLLQEEQIQEQLHMKPFTNMQQRLRVFSIFVIAIMVRSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPD9</accession>
    <name>T1GPD9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190445">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190446">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190448">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005467-RA">
        <property type="protein sequence ID" value="MESCA005467-PA"/>
        <property type="gene ID" value="MESCA005467"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1356038_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005467-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="22895" checksum="6D0CEE0DD71CDAA8" modified="2013-10-16" version="1">MNHLECLVVKDHELSFPYIYYSYLTDSALSHIACYVVAYSEKYYILIKLHHLLLAEEQNLHVSDILMLGGTNADQMTVETLNTPHPPKLYNFIVYVQSELHFIFGLRISFNIPYYIDAVSGLSPIPSGSLRWSCRWGNKEFSSRNIGSNELDIITPGGTHSGIKSYASSALRAHILEASTAMSEQMSCLKRTDFSIRRHTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPY9</accession>
    <name>T1GPY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02141905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA005685-RA">
        <property type="protein sequence ID" value="MESCA005685-PA"/>
        <property type="gene ID" value="MESCA005685"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476790_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007931">
        <property type="entry name" value="TsetseEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05267">
        <property type="entry name" value="DUF725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577075" description="Protein TsetseEP domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Protein TsetseEP" evidence="3">
        <location>
            <begin position="57"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005685-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20087" checksum="A4279852262F6C67" modified="2013-10-16" version="1">MXVFVAVFLALCALGVNAKPLKNPSEVSHLSPNQLLSYVQHVETISRAVRDGADKGQCFDYYLPKLAEATAEMDANRKLCGDVYDVEETKKLVELQSSKSAADGSSDDLNTKLSKCKDEEDPQKSLECHSESNDKNNCLIDVSTKGNSKSQEIYDDLFNCIATGEIKGEESNEDEPEEDLSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ30</accession>
    <name>T1GQ30_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02031865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ30"/>
    <dbReference type="STRING" id="36166.T1GQ30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005728-RA">
        <property type="protein sequence ID" value="MESCA005728-PA"/>
        <property type="gene ID" value="MESCA005728"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1483519_0_0_1"/>
    <dbReference type="OMA" id="VFQEPQA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027973">
        <property type="entry name" value="FSAF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052852">
        <property type="entry name" value="SSU_Processome_Comp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28366">
        <property type="entry name" value="CHROMOSOME 1 OPEN READING FRAME 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28366:SF1">
        <property type="entry name" value="CHROMOSOME 1 OPEN READING FRAME 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15375">
        <property type="entry name" value="FSAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="21910" checksum="0BDFB3B82AF46C52" modified="2013-10-16" version="1">MSDDLLDVVPTRAALLKKTNSEFKATVFESKKPEANPQKKKIQPQPDSEPEDDEDDIFSDRPRKRPTQKTDESEFNIKKARHEVINFAMSGFRSEKHKKKTEIALAVKLGARPAKKSYKNYKQLLQEKKNLKNVREERKKFHQLGKTQTGLASVKCKSKTQKGKDSKKRAPVSNIGMHYGKANPKFKSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ77</accession>
    <name>T1GQ77_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GQ77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005780-RA">
        <property type="protein sequence ID" value="MESCA005780-PA"/>
        <property type="gene ID" value="MESCA005780"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388124_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018061">
        <property type="entry name" value="Retropepsins"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00077">
        <property type="entry name" value="RVP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="3">
        <location>
            <begin position="389"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="280"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005780-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="712" mass="82486" checksum="58678037145926F3" modified="2013-10-16" version="1">MDSDPQNYVMDTQLVNLLLNNIPQFSGEGPNMEKDLAEFLRISGAISLQYATQQDTIVFVMKIKLIGRAQQALENKRITCFLDIKNALIQKFYSTEKVMDAYDNIKQQSNESVNEYGRRFMESYDRYIEKMMLSESNEFQKIAKRKFRCGLLSPELKHEAMNREIEKGVSLQDFISVIGKLDATYHSVEQQPLPSKSSSTENSEILEQNRILHKLLDMYAQNANTNQNQRQYSVHNGKTNRSQFYSKRITTTILTNXXXXXXXXXXXXXXXXXXXYNNFNQQQQNNNYNNYTIPKNNHYNNTNYHNSSNYNHNNSNNNNAYNNNYTANRMNNNYNNTSNNNYTNRTDNNYNNSQSANYNRSQNVRSNNNREKGPLPYFHHKFAITNNQIKTLIDTGAEISLIKLDSLSDRAKMNITQTNVHTIKGISNSEINPLGTLTTTLCSMPITFYVVKNEDTPIPGEALIGMTVLRWSTIDFPEGILKFKHKGFDNSDQCIKLTLETLTNKEKFINKVEQDIKFSEKDMPDKDRGIFKDIFANAHQIFRDDKDNTKYTPNELLYGFNPKIPCSIKTKTETVYNYDDYSRVMKLKFQEAHQMARENLLKSKYNSKEQYDKDCRERCLKIGDWVRMKNMTKEGGFPWRNYNCKNSNHPGCGTFDIRTATSGHEEIINFSRFGSLTINVSICGQKLSVPVFTNQYHNSINWTCLGKNKALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQE3</accession>
    <name>T1GQE3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005847-RA">
        <property type="protein sequence ID" value="MESCA005847-PA"/>
        <property type="gene ID" value="MESCA005847"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906689_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7299" checksum="59005C18C9708602" modified="2013-10-16" version="1">MEILGNRHQERKFYQKVNMRHPPASSSCKDKNGNLITHQQRYCIFLGAILKKLAVRQSITHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQI9</accession>
    <name>T1GQI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Aminopeptidase</fullName>
            <ecNumber evidence="25">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="30">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.</text>
            <dbReference type="EC" id="3.4.11.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23 25">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23 25">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 25">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="25"/>
    <dbReference type="EMBL" id="CAQQ02184916">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQI9"/>
    <dbReference type="STRING" id="36166.T1GQI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005897-RA">
        <property type="protein sequence ID" value="MESCA005897-PA"/>
        <property type="gene ID" value="MESCA005897"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003705_0_1_1"/>
    <dbReference type="OMA" id="WNVWSQF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004230">
        <property type="term" value="F:glutamyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000003">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000016">
        <property type="entry name" value="Glutamyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF276">
        <property type="entry name" value="GLUTAMYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 25" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12 25" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 23 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 25" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9 25" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13 23 25" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="28">
        <location>
            <begin position="88"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="26">
        <location>
            <begin position="292"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="27">
        <location>
            <begin position="578"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="22">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="24">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005897-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="901" mass="103661" checksum="8CD7A8F4E1FA76D8" modified="2013-10-16" version="1">MVAAAKIVTISLVLALTAFTVSTIVLAVQKANLNSELKEAREKINTLEKITTPAIQGSSTTPSITTTEAPAKVDYRLPQNVLPNLYNTIDLNIKDSTKNIILHSHLLKITKVQVLDGAKEIKSTFSLDTIKEFLIVDVDETLAAENDRYKIRIEFNGDMDDKIIGLYSSSYLKPDGTRKTIATSKFEPTFARQAFPCFDEPAMKAKYRVTLVTPTEDGYHALSNMPEKSSNDIGNNLKEVLFEDSVPMSTYLSCFIVSDFDYIEETVKANNVGNDFQMRVFATPEQKNKMDFALESGVKITEYYIDYFKVEYPLPKLDMAAIPDFVSGAMETWGLVTFRETSLLYDSKISSTANRQRVASVIAHEVAHMWFGNLVTMKWWNELWLNEGFASYIEYKGVDSAFPEWKMLDQFPISTLQGTVENPDQITEIFDTITYSKGSSIIRMLEDFIGPEIFRKSVTKYLEDNKYGNTETADFLDAIDAQEEVTINVKEIMRTWTEQMGLPLVEVEKLSTTSFRLTQKRFFSNPEDYNGVYDDSVFNYKWSIPITYRTNLNSEVKREWFYYNQESIDIVLDAEPQWIKFNSDQYGYYRVNYETEMWESLKNALLTNLNAFTITDRAAILNDAFSLADSTTIPYETSLDMTKYLKDETEYVPWVVASSKLTSLKTSLMFTDIYKEYITYARALIAKVYQEVGWEVDNVNEHIKNRKRVNILSSACSLGVQDCLEKASEKFLNWISDPKYDISSDIRELVYYYGMFNSGSEQIWEIVWDSRSIPWLLQRYIDLAWEEKYVRGQDYFSCLQNIASNRVGESLVWTHVREKWLDLVKRFGLNERYLGSMIPSITGRFDTQTKLEEIEAFFTKYPEAGAGAAARVRALENVKNNIKWLENNKEKIGEWLQKNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS5</accession>
    <name>T1GQS5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005989-RA">
        <property type="protein sequence ID" value="MESCA005989-PA"/>
        <property type="gene ID" value="MESCA005989"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576581_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005989-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9496" checksum="7B6BCB1F3F8B9A54" modified="2013-10-16" version="1">MFFVGELSSSSIRIRFEFPNSKRFSSISIFKDNFPPLSDWIDSQEIVWIFIGLVEFSIDMEVFAFRTVNFIWDNCAISGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSS9</accession>
    <name>T1GSS9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02083700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSS9"/>
    <dbReference type="STRING" id="36166.T1GSS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006740-RA">
        <property type="protein sequence ID" value="MESCA006740-PA"/>
        <property type="gene ID" value="MESCA006740"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362108_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006740-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10346" checksum="0FC0629DE1E4AA66" modified="2013-10-16" version="1">MSLPTDLSMKPNLQTQPMPMCSTSTPGNIGTCSTTSAAAISGTNQSQIPPLITHKLSPNEMTAARQVISAYRESAAFLLRTADQVEQLLIQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSV0</accession>
    <name>T1GSV0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GSV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006762-RA">
        <property type="protein sequence ID" value="MESCA006762-PA"/>
        <property type="gene ID" value="MESCA006762"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_788887_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006762-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="39813" checksum="9AA263EF25C8B126" modified="2013-10-16" version="1">TIQLEKCFYNICWIDFELCYIWGNDATLKXXXXFIIFAGLILNCAIFGAMMRPLKYPKESNEKPLMQRMYEEKKAQMERGSMGGSYFTVQLPDGTYEKRLKMPLNADPGVHSSLNLEQLAATLQPVATLPTITEQQAVQTTTTSASLKRKPPQTKRIHRNSETDASENSNSKSKSIIRNVSQPTFAIRQQGLPKNTSTPTFDRVRKISATEQPTFETTQRTRKISVGDRYKSIKSNLSKTSLASSIIGSTSGTSEQNKSRMVRPLSRKDIFYSGSVTNLQQYHQSQKSLTTYRQSVVSLTKYEKAMRSSQYIDRLAEAEANRENYALCPCCVIPESFRDALVTMLDINLLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSW3</accession>
    <name>T1GSW3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GSW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006775-RA">
        <property type="protein sequence ID" value="MESCA006775-PA"/>
        <property type="gene ID" value="MESCA006775"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059863_0_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF192">
        <property type="entry name" value="DOUBLE HIT, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="60"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006775-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="19216" checksum="7ED112788BC99C13" modified="2013-10-16" version="1">LAVKNGEDNIPKKKTESSELFLDGEQPTLEEVRSWGKSFDKLMNSSSXXXXXXXXXXXXSFDKLMNSSRGRKVFRDFLRCEYSEENILFWLACEELKKETNEESIGEKARHIYDDYISILSPKEVSLDSRVRDVVNKNMVEPTPNTFDEAQLQIYTLMHRDSYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSY3</accession>
    <name>T1GSY3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147540">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSY3"/>
    <dbReference type="STRING" id="36166.T1GSY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006797-RA">
        <property type="protein sequence ID" value="MESCA006797-PA"/>
        <property type="gene ID" value="MESCA006797"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_466610_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="15"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="509"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="512"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006797-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="585" mass="65021" checksum="0A0D3EBB052C17E6" modified="2013-10-16" version="1">MERFFNSLKKKSNNFMAVTRTNVLIKRHSSGKVAEYLRTNSVWVNGPLATISANLFQTDCSKGSIHERDGEMFFQVHPSIRQRRLVPIPAVVYKSGFTAGYYPELDATVIAFGNIKETVSTIYVMPGQQGTAAPGDNLDRLEKTLIETAISKNSWSHLLNTLVERPGLEVQLPRFSHRSFINASLGLQKMGLKGLFNSDYADLTGLTGSANKDVHLSDVIQINTFRIGKLGKKGTSGSKVEPFPEPSINEITTEMAETTTINEKSEEITTMHSSSVEKDYMEEQTTTNPVVQEIDEYIESNRPDMPDMVDIDPGYLNADFEIPEQTAGEHVTAGSSYMSGMMNFNDNYSKDTLEDSNENTAEIDSLMSSFLNKIDSSNRLSDISSMNESFFTDITTENTLETTTLQQEVDLEKEPKDEINEKYEEEIESTTEHVTDIPEFTTMKSAEELTTFSPESTESADDTVEFLTDSSMEGSTEKIGTTVEPTEHTSYNIEPEEAYLKLGEKFHTQAASSSTSSSSTTTTTDSSRLEASSTTTTEAPTNTTKSVPNSRKPQPYHRPTLEIRKKPVVYSANNLNKPMVRPATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTB3</accession>
    <name>T1GTB3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145326">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02145327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006939-RA">
        <property type="protein sequence ID" value="MESCA006939-PA"/>
        <property type="gene ID" value="MESCA006939"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402167_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006939-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10597" checksum="7A137417DBE24A74" modified="2013-10-16" version="1">MVERSQPKKPIEHKSKRIPEGTKRDIKRKGGKLPNFIGKREEDLRKSKTSRQNQSPAGMNDWPNVNMGSHKFEVLRNLIYLGSKVASDIPASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTH3</accession>
    <name>T1GTH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTH3"/>
    <dbReference type="STRING" id="36166.T1GTH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007005-RA">
        <property type="protein sequence ID" value="MESCA007005-PA"/>
        <property type="gene ID" value="MESCA007005"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2055578_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF46">
        <property type="entry name" value="EPS15 HOMOLOGY DOMAIN CONTAINING PROTEIN-BINDING PROTEIN 1, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="74"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007005-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="14000" checksum="9D5974ECD5355483" modified="2013-10-16" version="1">MRKYASIEPTQQRFTLSLKSTSKKIVNADLELTIKPPNNKDSVKRPELPPLELEKNYQNEVKEKSTERIILRDRTPGQDFLEWCKEVTKECPTVKVTNHTTTWRNGMAFCGIIHHFKTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTJ0</accession>
    <name>T1GTJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
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            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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            <begin position="99"/>
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            <end position="308"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007023-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
    <sequence length="393" mass="43623" checksum="41CFDEFB0B88C4CA" modified="2013-10-16" version="1">MWNVFAYTCANIAGKCKSQSQVLLYMLVTKGSRWYCTNCQECDACDDDPADKGPCVLGCHTCHKVFHLNCLNPMPDKKLKNPWRCRYCLDHHITPGKRLSNRSNQQLQLQKQQAMEDKQRRKAAHAKYEATPAKRKRAGTPANSDSSPNSPRTLSVYGSRRDQSHVTNLSEDSDEDDDCNNGKSSGSLRENLDNKPSTSAIANDKMSKEKQKFFRQSAFNSKRQKTKTLSESSSSESSDSENSDDDNDDDDSSSSGSSSSSSSDGSGSSGSGSSSSSESGSSDNDDDDDDDDDDMDGNNDRSKNDKNSSNKKKSNSSSSEMMNLRKTPFQTSLETLRMRGVGDLLRWLQKIKLMKVFQKTLKLGEEAIEIKTKTRINTETSSLQVVRNPKILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTK5</accession>
    <name>T1GTK5_MEGSC</name>
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        <recommendedName>
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        </recommendedName>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Arthropoda</taxon>
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            <taxon>Endopterygota</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
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    </dbReference>
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            <begin position="143"/>
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        </location>
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            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007039-PA"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="43405" checksum="170ECA64F9585E42" modified="2013-10-16" version="1">MGNPEKSLSDDLSNCLIDFKKKVTITSLLNDYGGTLEQISSDLKKFIESEVKKQFHKHYLIRGFTDDNGNQLITIVDEEGLEKIQKILKKPITLLFSVEEVADGKEISNDLVDDHTFQPIQNKWITLHNVRRAAAAAVEKVPEETKPVIKQEPVKKETKQKDFFTAKPTPAAAPQKKTPPPKKDETQKSGIKSMFQKQAAKPKPVESAAKKVKEASLGNGSNKTPVKKIKEEPVVQHSPNIFDESSSDEEETLNAMKKELGSDEEMSGQEPSTSNKRRRIVDSDDEEEMPEKKQEKLETSVVEDGVYYVYEDVTKTYEDEDGFMVTKKERVRKAVQAPPEKVAEKKKEKAEKAEKEKKKTPPKTAEKKKPAVSAKGKITSFFTKK</sequence>
</entry>
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    <accession>T1GTW3</accession>
    <name>T1GTW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA007157"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1951241_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007157-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14549" checksum="E288C23E68524192" modified="2013-10-16" version="1">MCQQYQHKFNKATISGPIEVKPTHLATRQIYKEEDPTIFEGERQFQTPPHKSLTPVLPEITRGITFLPESDDDDLGLGVLQSDEQITPPQSVFKKIKTEPMEMAKKPIQEVKPPLQPPLTVMTQAVIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU59</accession>
    <name>T1GU59_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tubulin--tyrosine ligase-like protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185107">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185108">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="36166.T1GU59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007267-RA">
        <property type="protein sequence ID" value="MESCA007267-PA"/>
        <property type="gene ID" value="MESCA007267"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010131_5_0_1"/>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:microtubule cytoskeleton"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein-glycine ligase activity, initiating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051437">
        <property type="entry name" value="TTLL_monoglycylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870:SF2">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007267-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="84774" checksum="F88240E786EC49E6" modified="2013-10-16" version="1">MSYINSKKSDNRNAPFQNDQIDKMVKAIQSRPYQNPSVKHHNIHIVFPTPPGYTPKGTLSTNTIKSTTNTVNQIPDIKSVNVLRHPSKEFISTSEQKLFNIDLYREYRKIAHDANTNRKIFMVYGAYRTIRNGLLRRGWIEKLAPGTYRNLRAMSQHALLLKAKKGNDYEKVCLSKMIEHFPAYFIWQMKFNRDEYLNAFPYRNRIRRGRDFDFSTKLGFIGCAESIQWCTQDGKSELKCPRFHRLNLEEQANFLEDFRLTECFSIVPSLIIKFAIDRVNDFVKHSKHRDVDEPYYEKSDEQWDHFVTNSRNIIRKNEKFKCDKTDYEAYKPQIRKALEDAKEHWPSLQMDGYNNLWILKPGFSCRGRGIVIMRNYENIVSHANKNPKSKYMVQKYIERPLLVYKTKFDIRQYMLLTITDNINIWMYKDCYLRFSSQEFTLDSFMNSIHLTNNSIQKYCKNGKRSTNLPAHNMWSLSDFRAYLNNIGHGNVWPSIYEGMKKSCVAIVQASLVDTEIYHHSFEIYGCDFMLNENSNLNSSPDLSPSTVVTRRICPAALEDLLKVVVDLPRNPKSNTGNFEKIFSTPIANVSSYIPGLLVNGMPMRIHQEILPTKPKLKKRQALLKGKFGKKKRNKKVTEQGVLLNTEAAKEKSVEKLNKLVSEFIPNTPTPRSESKSKEFLNSKIKEALALHFTSKSGKESAVLQNNDKIVDEFVNVLQRTSQKGSSEKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUE2</accession>
    <name>T1GUE2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041593">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUE2"/>
    <dbReference type="STRING" id="36166.T1GUE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007352-RA">
        <property type="protein sequence ID" value="MESCA007352-PA"/>
        <property type="gene ID" value="MESCA007352"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025015_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF40">
        <property type="entry name" value="IONOTROPIC RECEPTOR 41A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="45921" checksum="A4E8BA719D74EF75" modified="2013-10-16" version="1">MVKYFLEGVESNILVNYCARYNCTIEIVPSEYGEWGSIYENYSGDGLLGMIPMKRADIILNSMYQWQNIYATCGITRTMSRSGITFLIPRPNDVVVRGDFGTQNSRKLPSANLPLQQDSLDGSPPMFSSRSVSIAYVHYRDLPQNSNHKILKSLSFGTMSSYQIFCSQSVNDDLIQTPKRIIFFSCFIIDLIITSAYGGGLATILTLPSFGEVADTLEKMVDFELEWGGITYDWLYSVEFSEDTTIINLLKRFHVYSAEKMANLSAKGEIGVILERLTFGLFSIPEFFTNDAAKHYTVSKEEMYFELCVAYVSKSWAHMENYNKFISMVEESGLPEIWEWQMGVKYIDDTILNTLMSSKHLGNIGEDEPVPLGMSNFSGMIIIWCIGIGVSTLVFCYEVTFGRKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUR7</accession>
    <name>T1GUR7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUR7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007487-RA">
        <property type="protein sequence ID" value="MESCA007487-PA"/>
        <property type="gene ID" value="MESCA007487"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323053_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11420" checksum="DE7765AE7F65C9B2" modified="2013-10-16" version="1">MQGAKGANLIALVIPMAVDRWTESSFLQFICILSAVHQTTAPYMMIGLTRPLNIQKLFNPEISQITFKKFLWKGMFTLYKTRRHLHFVITDTKCAPFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUW0</accession>
    <name>T1GUW0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUW0"/>
    <dbReference type="STRING" id="36166.T1GUW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007532-RA">
        <property type="protein sequence ID" value="MESCA007532-PA"/>
        <property type="gene ID" value="MESCA007532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_792962_0_0_1"/>
    <dbReference type="OMA" id="QMSHEEE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005790">
        <property type="term" value="C:smooth endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005219">
        <property type="term" value="F:ryanodine-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014808">
        <property type="term" value="P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006941">
        <property type="term" value="P:striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048581">
        <property type="entry name" value="RYDR_Jsol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399">
        <property type="entry name" value="B30.2/SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399:SF8">
        <property type="entry name" value="B30.2_SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21119">
        <property type="entry name" value="RYDR_Jsol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ryanodine receptor junctional solenoid" evidence="3">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RIH" evidence="2">
        <location>
            <begin position="126"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="350" mass="39896" checksum="6EE621E6E62C6AF5" modified="2013-10-16" version="1">MKKISLSALQESEEEGSGGDSDGKPGALSKLFNFINTVKELEEGPKEVEEPEKKTPEEIFRKILIKIITGWAEETQIENPKLVREMFGLLVRQYDTVGELVRALEKTYVINFKAKNDVAEMWVGLSQIRALLPVQMSHEEEELMRKRLWKLVNNATFFQHPDLIRILRVHENVMAVMMNTLGRRAQAQSDAPATTEGGEGVPHKEKDTSHEMVVGCCRFLCYFCRTGRQNQKAMFDHFDFLLDNANILLARPSLRGSTPLDVAYSSLMENTELALALREHYLEKIAVYLSRCGLQSNSELVEKGYPDLGWDPVEGERYLDFLRYCVWVNGESVEENANLVFVFLSVVQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVJ7</accession>
    <name>T1GVJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sodium-dependent multivitamin transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02146169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146170">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1GVJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007806-RA">
        <property type="protein sequence ID" value="MESCA007806-PA"/>
        <property type="gene ID" value="MESCA007806"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_11_3_1"/>
    <dbReference type="OMA" id="INIAYWV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577670" description="Sodium-dependent multivitamin transporter" evidence="13">
        <location>
            <begin position="18"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007806-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="25663" checksum="B7BB98C80E3392DA" modified="2013-10-16" version="1">MPVLPVAFSLMVSFMSAVTLLGVSMENYTFGTQFVVINFAYVIATPIATHIILPVFYNLRTASVYEYLEKRFGFPARLYASLMFSLQMILYMGIVLYAPALALEAVTGLNRVGSIFAIGLVCTFYSTLGGMKAVVMTDVFQSLLMFVAIFSVIICAGIKAGGLDVIWEAAEEGGRIEFDSFSFDPTVRHTWWGLIIGGMFTYLTLYGGLQSLVVALQLGIPSSLTSLSTPGSNKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW27</accession>
    <name>T1GW27_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007996-RA">
        <property type="protein sequence ID" value="MESCA007996-PA"/>
        <property type="gene ID" value="MESCA007996"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3052738_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007996-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="54" mass="6098" checksum="5FB94E7668C1DD34" modified="2013-10-16" version="1">MKSPVLIEDGSFSWGDGEVCIKNINISDDKGYNILFGLPYDKRKYQRVVNACSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW80</accession>
    <name>T1GW80_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW80"/>
    <dbReference type="STRING" id="36166.T1GW80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008052-RA">
        <property type="protein sequence ID" value="MESCA008052-PA"/>
        <property type="gene ID" value="MESCA008052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_28_0_1"/>
    <dbReference type="OMA" id="HEPLEYA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11054">
        <property type="entry name" value="CYP24A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="58231" checksum="DD2A63039C498B3F" modified="2013-10-16" version="1">MSVKSMSNYRLLVRQRAHLNYVRSSYATHVCPHQKETIDGVSLENALPYHEIPGPKAIPILGNTWRMMPIIGQYSISDIGKISSQLYTQYGKIVRFGGLMGRPDLLFVYDADEIEKCYRNEGPTPFRPSMPSLVNIKRKYVRTFLATLVELLECNHGEEWRQFRSKVQKPVLQLSTIRRYLSPLEVITNDFLERCENILDENSELPDDFDNEIHKWSLECIGQVALDTRLGCLEPNLAPDSEPQQIINAAKYALRNVATLELKAPYWRYFPTPLWAKYVKNMNYFVSICMKYIQSATERLKNKPQSAVGSAGEPSLLEKVLLSEKNERIATIMALDLILVGIDTISMAVCSILYQLSIRPEEQEKIFKELERLMPDPNTPLTIPLTLQEDTVICGYRIPKGIQVVFPTLVTGNMEEFVSDAATFRPERWLKQQHGGIDGKLHPFASLPYGYGARMCLGRRFADLEMQILLSKLLRNYKLEYHYEPLEYAVTFMFAPDGPLKFKLLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWT0</accession>
    <name>T1GWT0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02122550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWT0"/>
    <dbReference type="STRING" id="36166.T1GWT0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008260-RA">
        <property type="protein sequence ID" value="MESCA008260-PA"/>
        <property type="gene ID" value="MESCA008260"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1005728_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008260-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="277" mass="30223" checksum="3571E77CFF2E76B3" modified="2013-10-16" version="1">MDDLGAEHLRLVTGLLRRRQGESRRSPENIKKIDDVISTLPNANEVIITSANTLTGVSPVIEGNKNEMDESSTISQKSAMAFTIDFGGNSDNSLAEQQAAKYKNMVERFQNRHKRGASMSKLESPGESAVTTPAVAPTPPQPAANTTAAKVKMRIRERSTSGVRDSSKRHSWSPRSSTHEASAPPVLPSPPSKVTAKNTVNIQKSLPIKKPLPPLRKKDSGPQSSFTPKSLAMQKAMEKFELYLPEPPLISNGKNLGEADGISEADIRYCDFMKNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWT2</accession>
    <name>T1GWT2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWT2"/>
    <dbReference type="STRING" id="36166.T1GWT2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008262-RA">
        <property type="protein sequence ID" value="MESCA008262-PA"/>
        <property type="gene ID" value="MESCA008262"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_573161_0_0_1"/>
    <dbReference type="OMA" id="FNIHTED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF41">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008262-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="477" mass="56500" checksum="AF65BD40CF2F94D2" modified="2013-10-16" version="1">FIATPEILSFEDLIDAIWKIPNITFNIHTEDFPYSEQNEVAVHHHVRELLIVTTKSLRFRYKLRKHIKSRLVILVTSKSTEQILERPYSLGLFNGIIFQNGTPFIFIGAENNIVLSFICPEISWPFFSVNEKLLLEEENNFFPLLIPLENRISGVFAYFAYSFRDYLYDKVMAKLIEFKKPFSERIVDEMIFSFVFTRRSDGYPLFSTKQCLMLPILDEISIQEYLRKPFTNWLWLLLVIFIIYISGILRGFIHEDSFQCFFESLTISCGSIYKGINSRNSRNRIIYLFLFLYGFVVWNLYSAKISSFLTTINRGRNLRTINDINNEKLVILASIPLFRSDKSLKWAFPEIYSFMRKFPDKRFLHNVSKNEFNELWLSMNNSFGYLIADYSWSYVSLIQSLLSRKLFSFSNLCPDKGFIYPTYFISKYVKLDETMDLFHMRVLENLKYFTIAWYIVFLGLGISAIIFILENLIHEHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX92</accession>
    <name>T1GX92_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ras suppressor protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX92"/>
    <dbReference type="STRING" id="36166.T1GX92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008434-RA">
        <property type="protein sequence ID" value="MESCA008434-PA"/>
        <property type="gene ID" value="MESCA008434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_18_15_1"/>
    <dbReference type="OMA" id="FNACENT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12343" checksum="7734691C677B68E7" modified="2013-10-16" version="1">MGNKQIKQHLETAQKTGILKISLQRLQDFPPQLKTYPNVLKTLDLSENRFEIIPDDIGRFTLLKHLNLSGNRLTALPDVLGNLAKLEVLLVMDNFLERIPRTLANLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXM4</accession>
    <name>T1GXM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008577-RA">
        <property type="protein sequence ID" value="MESCA008577-PA"/>
        <property type="gene ID" value="MESCA008577"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870191_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008577-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7734" checksum="E406FD5AE49E1BF3" modified="2013-10-16" version="1">MNLNIQGSIEAKFIHLMKPQRESGWRDHEYDKDVAEKDKLYRQRPGPQRKLKDRKMRKVVKLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXM6</accession>
    <name>T1GXM6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXM6"/>
    <dbReference type="STRING" id="36166.T1GXM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008579-RA">
        <property type="protein sequence ID" value="MESCA008579-PA"/>
        <property type="gene ID" value="MESCA008579"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827511_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF14">
        <property type="entry name" value="BEATEN PATH IV, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008579-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15833" checksum="DB55FCBEC2EA5E08" modified="2013-10-16" version="1">MARNLSMTSDDISYFSDINLIGICKKLTVQQLFSTWSIIHLFVVDDKVTSSDALIRYNDTVHKHGLITSTLGLQMTVEGRYFKNGPMRVKCLASISPVLWKGGRESIVQRRPGVIDNREAMLLDMEEIVSVTHTVQAGFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXR2</accession>
    <name>T1GXR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02016792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXR2"/>
    <dbReference type="STRING" id="36166.T1GXR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008617-RA">
        <property type="protein sequence ID" value="MESCA008617-PA"/>
        <property type="gene ID" value="MESCA008617"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1027756_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF154">
        <property type="entry name" value="PEPTIDASE M14 CARBOXYPEPTIDASE A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="3">
        <location>
            <begin position="12"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008617-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="271" mass="31356" checksum="3E300634CF870516" modified="2013-10-16" version="1">MEIIKPDIKHRYLRHNDINNYLKYLAKRYKYFVKVFNITKTFEEKHFRNLDLLSKLSIKNGEKIVVLILKVNALALIVIETMILCGNLKTNLRELSVHLKENIPFQKRKLVASKTCWKSSLAKFYSFCLYIHTLNLSCTLGHIQIAEAGYNAIKKSTGREYKYGSISSVTKRIFSGTVVDYAFGVINVPFCLVMELPNSKYGFQPPTDHIESITEESWIGIKAMCYQSLSCFEKVLFIPQKLFPQNISDAENNNQDFEIKNLNYNLKIVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY40</accession>
    <name>T1GY40_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Inositol oxygenase</fullName>
            <ecNumber evidence="4 13">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 13">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="12 13">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 13"/>
    <dbReference type="EMBL" id="CAQQ02379377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY40"/>
    <dbReference type="STRING" id="36166.T1GY40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008760-RA">
        <property type="protein sequence ID" value="MESCA008760-PA"/>
        <property type="gene ID" value="MESCA008760"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050259_1_1_1"/>
    <dbReference type="OMA" id="RYNTKYG"/>
    <dbReference type="UniPathway" id="UPA00111">
        <property type="reaction ID" value="UER00527"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF0">
        <property type="entry name" value="INOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="7 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008760-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="266" mass="30636" checksum="F3ED726D8BE5471A" modified="2013-10-16" version="1">MLIYKKYVKIFICLLRASAPNTVTLKVSGTGIHLWITRAASGHNSQSKFTYIPPVGLIDPSELQRPEPKFADKEISMFRDYSIDDNDPIKERVRRTYRNMHLNQTVEFVRNRRDVWLKFNHFKATVREALEKLNDLIDESDPDTSLPNIIHAFQSAERARQEFPEHDWLHLTALIHDLGKVMAFYDEPQWAVVGDTFPVGCEWGQSIVYRQDSFEGNPDGENPLYNTKYGMYEPNCGVDNLLIRGATMNTCIKCLSITSLHCLKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYC0</accession>
    <name>T1GYC0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYC0"/>
    <dbReference type="STRING" id="36166.T1GYC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008844-RA">
        <property type="protein sequence ID" value="MESCA008844-PA"/>
        <property type="gene ID" value="MESCA008844"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929963_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008844-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14098" checksum="7B3C15546C505610" modified="2013-10-16" version="1">MPHPPTPPLQRRLAKSFSVAPTLAQQKGIYQTPPFHTDDIVQELRGLSSQTSVSSVENSQNIATQISSTSHTTQHSHSHFSLSGSATSAFNALMGGHHHHHHHHHNVEAGNVGQNPGSIGADHPNIIMPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYC8</accession>
    <name>T1GYC8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYC8"/>
    <dbReference type="STRING" id="36166.T1GYC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008853-RA">
        <property type="protein sequence ID" value="MESCA008853-PA"/>
        <property type="gene ID" value="MESCA008853"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_0_1"/>
    <dbReference type="OMA" id="CANIVQW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF32">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008853-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="21702" checksum="01ADC9B5BA7913A2" modified="2013-10-16" version="1">MGYIGLEEKTEEALDNDGWLHSGDVGYVDDKGYIYITGRSKEIIITAGGENIPPVHIENLVKKELPAISNAFLVGEQRKFLTILVSIKTEIDKDTGAPLDDLTHESLQWMETLKLKYTKLSEILEAGPCPIVTKAIFDGISRANKYSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKQYSDIIESMYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYT5</accession>
    <name>T1GYT5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009022-RA">
        <property type="protein sequence ID" value="MESCA009022-PA"/>
        <property type="gene ID" value="MESCA009022"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017865_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009022-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14785" checksum="DC90FF06B2DA5A05" modified="2013-10-16" version="1">MAHFNLSSERILPQSSDVKLPTPSCSTDTQEEYNDAVSVQVKLYRRSFKIKTQAAKEAYRSKRREVVKMSKLEKRRLEKQQFEELEFMRNRNQATKFYQNVNKQRKYDTPIFVKIEICFPKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYV7</accession>
    <name>T1GYV7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein O/MICOS complex subunit Mic27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02386583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYV7"/>
    <dbReference type="STRING" id="36166.T1GYV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009044-RA">
        <property type="protein sequence ID" value="MESCA009044-PA"/>
        <property type="gene ID" value="MESCA009044"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080820_1_0_1"/>
    <dbReference type="OMA" id="LCYPRQA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033182">
        <property type="entry name" value="MIC26/MIC27_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14564">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC26 / MIC27 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009044-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="23551" checksum="5CAA356F6A25800E" modified="2013-10-16" version="1">MFRKVVLTSPVVFGAVAVTQKAPVESKEASLAHYQCKPSDLPVYAPLHSLTKKEPKCPHPHQPKQAAPIQALECGIRTVRVEVFNVCNALSEQKKNFDDVLETGRAHTQTTIDYLNEPANTMPRVGAIAIGGLAGMIFAVRGGFIKKVLYTGVGAGGIASLCYPKEAEHYGQIALQEAKKNFAIGYNFAMGIKPDDSNKKLPEAVSKFPTSLRIFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ17</accession>
    <name>T1GZ17_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ17"/>
    <dbReference type="STRING" id="36166.T1GZ17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009108-RA">
        <property type="protein sequence ID" value="MESCA009108-PA"/>
        <property type="gene ID" value="MESCA009108"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164133_0_3_1"/>
    <dbReference type="OMA" id="GRANIMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000004">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF380">
        <property type="entry name" value="COLOSTRUM TRYPSIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="4">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009108-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9727" checksum="38884EEC411D5C16" modified="2013-10-16" version="1">MLKTLSVTMKVLAYLIAILAIFSMVTASIPTRCKLRRVTGPCKASHRRYFYNYNLHKCQSFNYGGCQGNLNNFHSIEDCIKVCMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZH3</accession>
    <name>T1GZH3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZH3"/>
    <dbReference type="STRING" id="36166.T1GZH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009274-RA">
        <property type="protein sequence ID" value="MESCA009274-PA"/>
        <property type="gene ID" value="MESCA009274"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_545481_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588628" evidence="2">
        <location>
            <begin position="20"/>
            <end position="500"/>
        </location>
    </feature>
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        <location>
            <begin position="227"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009274-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="500" mass="56768" checksum="2601B2766FD24E1A" modified="2013-10-16" version="1">MKILSKLFIFILFIEISFQKVYNAKHLCKQKFVTTNILANKYISTTDLMGKSSQYYFVLNTTVVVRVGGPGMAVHIGFHKTKNIESSKFYNELEIMYSMTYIKGQLHSGSTIQAIGGKCVLEKLLTRSRVSWFATYIILTEKKLVVYYESYKKPVISCDYYSTQEFFPNYINVYTQRKDQPTKFYYQCENFLRSLIPPVFNYKDKSRKNLLTVYDKEDTDILLNKNETKPTKAPKTTTKKPTTTTKVPTTTTKVPTTTTKVPTTPTKVPTTPAIVPTITTKNDEPELSTRQIEKAKLQISTEGPKDSNLEVSSSNIYFTVYTTERTTGNTDFVSLNEEYESSREESKSSTEESEFYKKEYKTESSNEESESSTEESKSSTEESKSSTEESELFNEESESSTEESESSTEESETSIEESSTEESESSTPESESSTEESSTEESESPTEKYGSSTGSSTAQSESFIEESKYSTEESESSSKDLETESSKSEYYFYSTKSTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZN0</accession>
    <name>T1GZN0_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009334-RA">
        <property type="protein sequence ID" value="MESCA009334-PA"/>
        <property type="gene ID" value="MESCA009334"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932821_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009334-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="15497" checksum="9E6C5113A4A63E1D" modified="2013-10-16" version="1">MISHIGDNIAELHHIAVTNCLESFYKFVPNIIPYITDKPVSILQKQCEIKCFFKIFENFMFNDPDQLSKRIQEIELDSKNSRTLSYPSYLSENIPEELYLAKKIYRKWFDDQEMLEHKYKDIIHIGEGGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZX4</accession>
    <name>T1GZX4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098734">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098735">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009434-RA">
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        <property type="gene ID" value="MESCA009434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3336126_0_0_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="38" mass="4463" checksum="49475404DDDEB859" modified="2013-10-16" version="1">MEVHNGEYDGYKDLVCEDKNTDFFVVPKERQRPITDCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZY2</accession>
    <name>T1GZY2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007436">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="MESCA009443-PA"/>
        <property type="gene ID" value="MESCA009443"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266797_0_0_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009443-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11632" checksum="263FB59327BC973F" modified="2013-10-16" version="1">MKDSSNFTVNLRSEENRLATESEGEVDSKSSQKRLTSLASTKIGIGDMNDHPSFSKIGSENRHDITEMMDNPTLRRNRNDENKMNSKEKTDDPSFSKPKLEXG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZY9</accession>
    <name>T1GZY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SURP motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZY9"/>
    <dbReference type="STRING" id="36166.T1GZY9"/>
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        <property type="gene ID" value="MESCA009451"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1379552_0_0_1"/>
    <dbReference type="OMA" id="HEMALNS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323:SF0">
        <property type="entry name" value="CALCIUM HOMEOSTASIS ENDOPLASMIC RETICULUM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323">
        <property type="entry name" value="SR-RELATED CTD ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SURP motif" evidence="2">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009451-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22290" checksum="9BB018AA28E6A293" modified="2013-10-16" version="1">MEPPPPPRVNFTKILCNSRTGQRNIIDKLAEFVARNGPEFESITKRKQQGNPKFEFLYGGEDYQYYKYRVAMEQAMLKNPQNVPQQPVFNSGVQNGGATVTPSDISNLTSQIETMKIQQHTLREQIQQSERNLTAQHTALMKQQTKQIEDAIVSAQNETLQAQADENGVDLTEFDGILQPIIDSCTKDSISAVPFLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H084</accession>
    <name>T1H084_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02162337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H084"/>
    <dbReference type="STRING" id="36166.T1H084"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009557-RA">
        <property type="protein sequence ID" value="MESCA009557-PA"/>
        <property type="gene ID" value="MESCA009557"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1367611_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004046">
        <property type="term" value="F:aminoacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052083">
        <property type="entry name" value="Aminoacylase-1_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892:SF1">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="1">
        <location>
            <begin position="115"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009557-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="22735" checksum="9F8DB79411CA2014" modified="2013-10-16" version="1">MFLNYIKIVLISCEDKVTECQLQEGIVLSCCWIIFLVCMSIVGECWDEEIGGKLGMKLFVETDDFKALNVGMSLDEGAPWPKEVSPIYYGERSTWQIHLKFHGQAGHAKHIGDVTTINLTKVLGGVQTNVVPSLLEAWFDCRVAVDVDMQKYEEMIEKWAKEAGENVEIIWDRKDPIVPPTCIDDSNKFWGAIKRSFEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0M3</accession>
    <name>T1H0M3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0M3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009704-RA">
        <property type="protein sequence ID" value="MESCA009704-PA"/>
        <property type="gene ID" value="MESCA009704"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888304_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009704-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7425" checksum="E97224D7DA14243E" modified="2013-10-16" version="1">MKERSRRGTNFLRILLITTRYDAISFLVLEKAACDIGLQANEERSNTYFLMEKKQAIEDWVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0T4</accession>
    <name>T1H0T4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0T4"/>
    <dbReference type="STRING" id="36166.T1H0T4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009775-RA">
        <property type="protein sequence ID" value="MESCA009775-PA"/>
        <property type="gene ID" value="MESCA009775"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2446992_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009775-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10353" checksum="B7AAF03CCB687EB6" modified="2013-10-16" version="1">MLWHVLICILYSSTEPEGKALLEDLSKLNKPLEITFEEMLKLMRQCEQIDKLNEKSKRKNRGEIIKEQNTSLQGSIFPKSPLSLLNGIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H137</accession>
    <name>T1H137_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H137"/>
    <dbReference type="STRING" id="36166.T1H137"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009889-RA">
        <property type="protein sequence ID" value="MESCA009889-PA"/>
        <property type="gene ID" value="MESCA009889"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1820624_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF9">
        <property type="entry name" value="NO MECHANORECEPTOR POTENTIAL A, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009889-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="15534" checksum="E7FD493CF28FB9AC" modified="2013-10-16" version="1">LRISGPQGYPECGTQRFGDTLTNIIVVQFSDNVQTSRDKRYNLTCVFRGPGEAVVTSGYIGAGSGSPIPIEYLPAENTLSSKVRLMILYQGRPTTTIAVGDPLTFRLEAQDGYNHITDIFATNVVARDPYSGRSIQLIDRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1I9</accession>
    <name>T1H1I9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N(6)-adenosine-methyltransferase non-catalytic subunit METTL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Methyltransferase-like protein 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02374309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02374310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1I9"/>
    <dbReference type="STRING" id="36166.T1H1I9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010051-RA">
        <property type="protein sequence ID" value="MESCA010051-PA"/>
        <property type="gene ID" value="MESCA010051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1965052_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14682" checksum="51772E3FC24A0DA2" modified="2013-10-16" version="1">RTVTSEGGFLNFHEDDEGTQSSNPHNDYCQHFVDTGQRPQNFIRDVGLADRFEEYPKLRELIKLKDDLISDTATPPMYLKTDVKSFDFKKLETKFDVILIEPPLEEYTRGAASVATIPGGAPRVFWNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1N8</accession>
    <name>T1H1N8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1N8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010105-RA">
        <property type="protein sequence ID" value="MESCA010105-PA"/>
        <property type="gene ID" value="MESCA010105"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_970678_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053729">
        <property type="entry name" value="MAD2L1BP_domain_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010105-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="29998" checksum="EE35C3258A3E2F13" modified="2013-10-16" version="1">MKKQNNKNVEINFSTEVLTKEQAGQIFIAIYELLLYQRNSIPFVYQTFKYLTSRLPQIDDEETNIHFDAQRQISQARKTLEDINNHFKRNANHIWIHPFNSQGSLILKTPKICNKHYNENHRNSSERLIKQILMQVVMSTEISDHFVSKSLPQTNVFLQFDMSISSEFGFSCTEKDFEDYNYCKNLVILNLLRTGESGILNCCSDLKILDDFEKFNLIEKKEEGNEEYSLYEVDHFIKGFKDKLVKGQSIWEITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H271</accession>
    <name>T1H271_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MORN repeat-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02117196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H271"/>
    <dbReference type="STRING" id="36166.T1H271"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010305-RA">
        <property type="protein sequence ID" value="MESCA010305-PA"/>
        <property type="gene ID" value="MESCA010305"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174269_0_0_1"/>
    <dbReference type="OMA" id="NDFRYEG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048678">
        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052315">
        <property type="entry name" value="MORN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010305-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9645" checksum="C3ACDC40FAE184D7" modified="2013-10-16" version="1">MGWFHGFGIFWRADGMKFEGEFRGGKIWGLGLVTFSDFTHGFPRNEGYFQDCRLIRKKRCPEIVQKAQKVALMARQQCEHPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X2</accession>
    <name>T1H2X2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2X2"/>
    <dbReference type="STRING" id="36166.T1H2X2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010578-RA">
        <property type="protein sequence ID" value="MESCA010578-PA"/>
        <property type="gene ID" value="MESCA010578"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029354_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF63">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010578-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13568" checksum="820DCE12D139A3B8" modified="2013-10-16" version="1">MLDNEELISTLENTKTKASLVTEALKLSAETSKDIEKLRNGYRPAAKRGAVLFFALSDMATGSVALTKSERQCPAKWINEKSWEDIMKLSNDFPDTFGSLPDSITIKLAEWKEVCIGTLIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H348</accession>
    <name>T1H348_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H348"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010662-RA">
        <property type="protein sequence ID" value="MESCA010662-PA"/>
        <property type="gene ID" value="MESCA010662"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888347_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7601" checksum="7033C594E4A1CD26" modified="2013-10-16" version="1">MKMKFQHWINLSTKTTLMMKTFLVFPEEYLINIIAECHRPSFGYIQYTYYGSRNCIVKVPIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H351</accession>
    <name>T1H351_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H351"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010666-RA">
        <property type="protein sequence ID" value="MESCA010666-PA"/>
        <property type="gene ID" value="MESCA010666"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640965_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010666-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8879" checksum="57B07089470D796F" modified="2013-10-16" version="1">MKSGMWEIDAVWHSRSRNLHALTAPCMQSKFPNTLSKLLSQALRIVKTWTIENGVNANKTKLVMFRRKHKIADITKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3B6</accession>
    <name>T1H3B6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[phosphatase 2A protein]-leucine-carboxy methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.233</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">[Phosphatase 2A protein]-leucine-carboxy methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:48544"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="2.1.1.233"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.233" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02198716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3B6"/>
    <dbReference type="STRING" id="36166.T1H3B6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010736-RA">
        <property type="protein sequence ID" value="MESCA010736-PA"/>
        <property type="gene ID" value="MESCA010736"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114331_0_0_1"/>
    <dbReference type="OMA" id="NDMMDRF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018423">
        <property type="term" value="F:protein C-terminal leucine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016651">
        <property type="entry name" value="LCMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600:SF33">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010736-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="23661" checksum="FEF958715B19F650" modified="2013-10-16" version="1">MGVDLRNLEEVEQKLISAEIDFRLPTIFLSECVLVYIEPKYSIPLLKWFSSKFENAAFVNYEQDPAPFNSVHQTCHWFLCMISYEDLLFELHSPLDTRWSAISLLYLIPEVNMNDRFSSVMLSNLRARGCSLAGVDFCLSLETQKNRFLSTGWSGCRAWDMIQVYQSISASDRHRIERLEMLDEGELLVQLFQHYCLVVAWKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3F0</accession>
    <name>T1H3F0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3F0"/>
    <dbReference type="STRING" id="36166.T1H3F0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010775-RA">
        <property type="protein sequence ID" value="MESCA010775-PA"/>
        <property type="gene ID" value="MESCA010775"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_26_1"/>
    <dbReference type="OMA" id="HIMEREL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF152">
        <property type="entry name" value="UT01108P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="7">
        <location>
            <begin position="105"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010775-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18097" checksum="E4BC04792F773BEB" modified="2013-10-16" version="1">MSLCGTPEYLAPEIIQSIPYGESVDWWSFGVLIYELINGTSPFHAFNKDPMTMFETICSGEFKMPNEFSGHLQDLIRGLLQVDVTKRLGHMKNGSRAIKEHIWFQDIDWHCMLNQTIDPPMIPNLKDPLDCSHFGKSPQMALPRSRTDQFAKQFEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3T5</accession>
    <name>T1H3T5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02386084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3T5"/>
    <dbReference type="STRING" id="36166.T1H3T5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010922-RA">
        <property type="protein sequence ID" value="MESCA010922-PA"/>
        <property type="gene ID" value="MESCA010922"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140770_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010922-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11568" checksum="72793AA02E19ECCE" modified="2013-10-16" version="1">MFSKLTRRAMSIAANKQPLNALGNVTAQQVFDREDKYGCHNYHPLPVALTEGEGVFVWDVNGKRYFDFLSAYSAVNQGHCHPKILNALHQQSKKLCLTSRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4A3</accession>
    <name>T1H4A3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4A3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011111-RA">
        <property type="protein sequence ID" value="MESCA011111-PA"/>
        <property type="gene ID" value="MESCA011111"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500467_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011111-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9590" checksum="A041BC42662E1913" modified="2013-10-16" version="1">MTIGGMETIDQDQGHRGLEETIKRRKLIKRILVNLVVVIPITTMTITVTGIAIAHQDRRDLQGHQVIILMIIGGGEIEMVTEMVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4U7</accession>
    <name>T1H4U7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4U7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011315-RA">
        <property type="protein sequence ID" value="MESCA011315-PA"/>
        <property type="gene ID" value="MESCA011315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834043_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7688" checksum="633967C5A8CA5319" modified="2013-10-16" version="1">MSRYRMVVWYRMVFLIIEIRSQLFIESVVKLAAKEEIEAGAKLNSKSGHLHIVSLRCSHDLSNRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4X0</accession>
    <name>T1H4X0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4X0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011341-RA">
        <property type="protein sequence ID" value="MESCA011341-PA"/>
        <property type="gene ID" value="MESCA011341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545184_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9590" checksum="1DCEE9FE11CBEC0B" modified="2013-10-16" version="1">MIDNHTQKLIRNVYLANTVRDNAKQVQFNCSDTQLSAPDIIGSLFPNLKLSISNRTPELTRGFFNPTKSFRLFDYNLCIFTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H537</accession>
    <name>T1H537_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02193514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H537"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011411-RA">
        <property type="protein sequence ID" value="MESCA011411-PA"/>
        <property type="gene ID" value="MESCA011411"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_954061_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303:SF27">
        <property type="entry name" value="CYTOCHROME P450 307B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303">
        <property type="entry name" value="HEME-BINDING MONOOXYGENASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011411-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33562" checksum="FBE86FA3BC4A49C5" modified="2013-10-16" version="1">MYISLWTISSNTLYLLFAFALLSVXXXXXXXXXXXXXXXXXXXXXXXXVVLVSEAINRRKKLISSKISSAPSPPGPKSFPIIGNLNVLDGYEVPYQAFTDLGKQYGQIIKLQLGSVPAVVVNGIDNIKEVLITKTNHFDSRPNFQRYHRLFSGNKENSLAFCDWSDLHKLRRDMLTPHTFPKSFSTRFQDLNGIISQHTEQMMNQLNQIPTQTVNIKEYFLKSSANIFNEYFCSRIIDYNDKQLDTYIKNFDGIFYEVNQGYAADFMPFLMTFHKSNINKWTGGRIKYEISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5K0</accession>
    <name>T1H5K0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02162511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5K0"/>
    <dbReference type="STRING" id="36166.T1H5K0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011580-RA">
        <property type="protein sequence ID" value="MESCA011580-PA"/>
        <property type="gene ID" value="MESCA011580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_851461_0_0_1"/>
    <dbReference type="OMA" id="WYKSARR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000144">
        <property type="entry name" value="Picot, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF280">
        <property type="entry name" value="FI21844P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="70"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="327" mass="36838" checksum="0AFDBCE0966D65B6" modified="2013-10-16" version="1">INKFSNILQKNIFLLYNLLSSRLLFDNFTLQKFHNSFRMNFSILLNIEFNNILLLGLFGRSGFRYVQTFLLFFGITAALFVRANVGIAVVAITDIKTEDHSYYSWTFDEKQLILSTFYWGYIFTQVPGGYAAQKLGSKIVLLLSILLSSLLSLVTPVSIIYGGWRAYCVIRVLQGLAQGVMLPSIYDHLAKWSPKEERNLQGTLALSGYSCGLVFGMGISGVIAASAMKWPGIFYVSGAIGVFWSILWIIFGASTPNSSRFITEKEKKFIESTQREDNNTTAKPNNQYHGGKSSHHHHFWLCCLYPAANHSEHSSPALKCPFISMQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H619</accession>
    <name>T1H619_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import receptor subunit TOM22 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom22 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H619"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012152-RA">
        <property type="protein sequence ID" value="MESCA012152-PA"/>
        <property type="gene ID" value="MESCA012152"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108175_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22884">
        <property type="entry name" value="TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005683">
        <property type="entry name" value="Tom22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04281">
        <property type="entry name" value="Tom22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012152-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="15980" checksum="5E9D97D4B9F0DE10" modified="2013-10-16" version="1">NLEPAKLEDLLDSDPDIEFIEKDSGMSSLSGSKGGGKSXXXXXXXXXXXXXEPDETLGERLWALTEMFPSPVRDFCGTPMCLATVKNLYTFSCNASWIIFSSSIILFAPVIFETERAQMEEMKKSQQKQVLLGPGSAGPTPAMPGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H622</accession>
    <name>T1H622_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H622"/>
    <dbReference type="STRING" id="36166.T1H622"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012155-RA">
        <property type="protein sequence ID" value="MESCA012155-PA"/>
        <property type="gene ID" value="MESCA012155"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098079_1_0_1"/>
    <dbReference type="OMA" id="SWIDYAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043517">
        <property type="term" value="P:positive regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10305">
        <property type="entry name" value="GST_C_AIMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053837">
        <property type="entry name" value="AIMP3/p18_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042450">
        <property type="entry name" value="EEF1E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44490">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 EPSILON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44490:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 EPSILON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012155-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="17072" checksum="5C63B2D8FB6A61B9" modified="2013-10-16" version="1">SEDIRGFCTILHQILKTCDHEVAKNSVNSPEILAQVQSWIDYACLYIWAGKDDKHIWKQLLQELNQYFSDKSYFVSENLTIADIVVFYALYDLMKKQTPVDKENLLNLSRWFDHLQQISGVRQNLEPLNFTTIYLHGWATGTRGQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H670</accession>
    <name>T1H670_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H670"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012203-RA">
        <property type="protein sequence ID" value="MESCA012203-PA"/>
        <property type="gene ID" value="MESCA012203"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134620_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="12585" checksum="BF7B32A0C9182A27" modified="2013-10-16" version="1">SGGHLQVKKGSSVRIECSASGNPTPNVTWTRKNNILPNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPPEIIVEQPVVFSGEGREATLVCIIHGVTEPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H672</accession>
    <name>T1H672_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin-related protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin family. ARP6 subfamily.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H672"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012205-RA">
        <property type="protein sequence ID" value="MESCA012205-PA"/>
        <property type="gene ID" value="MESCA012205"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_510549_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000003">
        <property type="entry name" value="Actin-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="60704" checksum="C0702263133366E2" modified="2013-10-16" version="1">IDNGAYTSKVGLSTDEAPRIIPNCVMKAKSERRRAFIGDQLKDLKDASGLFYLLPFQKGYLVNWDIQKTVWDHIFSPDSSGINLSESNVIITEPPFNFPTIQEGMMEIFFEERCRVLHKIAASKLAAIHYANIGPNFQCIIIDMGFSFIHIIPFXGKVYKGIKRIEVGGKVLTNYFKEVISYRHLNMMDEGYVVNQIKEDVCFVSENLNEEMEKRKEFIEYILPDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSESIDWSVEIGICDRCADKSSSNYHAWSHRLWVLQRAPAFSEVILTEKFIKKHISDYSCYHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6I3</accession>
    <name>T1H6I3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6I3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012316-RA">
        <property type="protein sequence ID" value="MESCA012316-PA"/>
        <property type="gene ID" value="MESCA012316"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2580684_0_0_1"/>
    <dbReference type="OMA" id="MGTARRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012316-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9155" checksum="D0BC652202229997" modified="2013-10-16" version="1">NKPKPDDDIPEFDRHNVQLDIPPQLFSNSFSTVTKVSDLISKLIMNSSRRYAKFALFFKPLFGDALTVEGYQDPTTTTSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H756</accession>
    <name>T1H756_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 208</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM208 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H756"/>
    <dbReference type="STRING" id="36166.T1H756"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012538-RA">
        <property type="protein sequence ID" value="MESCA012538-PA"/>
        <property type="gene ID" value="MESCA012538"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094308_3_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012538-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10273" checksum="D6F93A993CE648BF" modified="2013-10-16" version="1">PPKGKQGTKGAKQIVQENKETLKFYKIMNSKCCFLLVTLVFFEFTTWITVLTIIAFAALFGAYQFMAFMPRPKLKMVTLDSGTSLNIEGGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA18</accession>
    <name>T1GA18_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA18"/>
    <dbReference type="STRING" id="36166.T1GA18"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000061-RA">
        <property type="protein sequence ID" value="MESCA000061-PA"/>
        <property type="gene ID" value="MESCA000061"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066677_0_0_1"/>
    <dbReference type="OMA" id="KSECPEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000061-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="261" mass="29388" checksum="359D249707E10F3E" modified="2013-10-16" version="1">MSDREPYYGPPPDSDSDESYEGFYFGDDHIDKKANRRAQLVINPDQELYDSAHSGNLESLKKNIKDLMFDLNEILETGGQTLLLVACRFGNVEVVEYLLKNGANPNRQVDSYSPLMEACSLPIEKTKEAYDIVKLLLNYKVVVNVSNKFGMTPFMFACKYGHIAVVKKLMKLASMEAADNNGTNPLLHAIENNQQEIVKILLQEGASIEVCNRKGYSPSQVAQFFGFYDILDLLPKEKETFFVPSTFLCYNSLSDGFQESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA57</accession>
    <name>T1GA57_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA57"/>
    <dbReference type="STRING" id="36166.T1GA57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000103-RA">
        <property type="protein sequence ID" value="MESCA000103-PA"/>
        <property type="gene ID" value="MESCA000103"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838559_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7523" checksum="F43AEE3D5E2E4957" modified="2013-10-16" version="1">KVLRELEIKKPQLNELVNTAETLKADTNRQQLQDKAPERKLTPKPNTPYCLVVKNGESSSQTSWCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB38</accession>
    <name>T1GB38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB38"/>
    <dbReference type="STRING" id="36166.T1GB38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000459-RA">
        <property type="protein sequence ID" value="MESCA000459-PA"/>
        <property type="gene ID" value="MESCA000459"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363407_0_0_1"/>
    <dbReference type="OMA" id="HGITECC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF324">
        <property type="entry name" value="SOX DOMAIN-CONTAINING PROTEIN DICHAETE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="66"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000459-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="22719" checksum="99DB5C72CD0ADFE6" modified="2013-10-16" version="1">MMDRIPSLALSGISLAHPLSISSNQNIQNHLSSSSIAHQTSQHNTNHHLNLHSTTNSGLSNNGAPTSSIHHSTTAHHQQQQQQQQQQQQQQQHHSQSNSSNNHHPHSTHHPQHSSTSHHHHHNSQTSAMTNLHQNMGNLMNSGSSASDVFFSLMIQNTTKRQVEEHIKRPMNAFMVWSRLQRRKIAQDNPKMHNSEISKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC73</accession>
    <name>T1GC73_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC73"/>
    <dbReference type="STRING" id="36166.T1GC73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000874-RA">
        <property type="protein sequence ID" value="MESCA000874-PA"/>
        <property type="gene ID" value="MESCA000874"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362130_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000874-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11082" checksum="8A644BE1732A601A" modified="2013-10-16" version="1">MGAMPKTFKLPIAEYTSPGVLSYYVEHVVDIVQYPDAKTELFKLFREFGNSTSRRSSFVKPYCEENEKPEDKHKRLEAQFTNLKNFADVEKLGTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCF7</accession>
    <name>T1GCF7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neuroguidin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCF7"/>
    <dbReference type="STRING" id="36166.T1GCF7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000968-RA">
        <property type="protein sequence ID" value="MESCA000968-PA"/>
        <property type="gene ID" value="MESCA000968"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031901_0_0_1"/>
    <dbReference type="OMA" id="PVHYNET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000968-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33575" checksum="688DAA565DCFBD6E" modified="2013-10-16" version="1">MSEEISLSEEGRYRALDSDLEIQPDLPQALKLIHEMNDNVKQVTSLVESMLEKVRSGELSTEYGLSFLEVKYHMLLDYLINLTYIVLRKTSGDEIDGDPAIDRLIEIRTVLEKIRPIDQKLRYQIDKLVKTANFGDDDDEEDDDEGSDEDDDEKDDSDEDEDDDVEEDGVEAIKTSKNKGASKNKSGIYVPPKLRPVHYDGDETQAEKEKRVIERAKKRAMTSSMLQDLKEELLDAPLEVSSGSRAQQIFSKQQKEKEEYEEAYLTRLPVTKAEKHRQRKLTTLGNLYFFIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCR1</accession>
    <name>T1GCR1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001081-RA">
        <property type="protein sequence ID" value="MESCA001081-PA"/>
        <property type="gene ID" value="MESCA001081"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415773_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588196" evidence="1">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001081-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10138" checksum="E6DC10F442870703" modified="2013-10-16" version="1">MYMAVMGLLSVILVLFCDLPNILSNAPRVNLPGQGDILGSWSETAWTNQKFQQFLSIPYAESPKGSLHLRILQHVPNGGRTLIDDLQICSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDK8</accession>
    <name>T1GDK8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001397-RA">
        <property type="protein sequence ID" value="MESCA001397-PA"/>
        <property type="gene ID" value="MESCA001397"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1837378_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001397-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16090" checksum="386C9CF8680AF7B8" modified="2013-10-16" version="1">MRSLNFKTGIAFGYSRYGNLLETVYGTTTTEILGMQKSHRPSGGNRKADKKAFFIILPIAPSRSSLSFLHPWVFGFVDGGILLYDPFTSRIASYKEPQSFMQYTVTLFIEHPDKSNHHDYERKKNSVLRSLTTMDIDWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDS2</accession>
    <name>T1GDS2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDS2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001468-RA">
        <property type="protein sequence ID" value="MESCA001468-PA"/>
        <property type="gene ID" value="MESCA001468"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944334_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001468-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6590" checksum="2A2B90D71F92C371" modified="2013-10-16" version="1">MYVVGFEAEVHAIVLVVILEKIVSHGTSHANRRHVKTVEPVDQQIANPTSATVLLSSYPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE06</accession>
    <name>T1GE06_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adult cuticle protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE06"/>
    <dbReference type="STRING" id="36166.T1GE06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001562-RA">
        <property type="protein sequence ID" value="MESCA001562-PA"/>
        <property type="gene ID" value="MESCA001562"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148241_0_0_1"/>
    <dbReference type="OMA" id="AWNNGWA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031874">
        <property type="entry name" value="Cuticle_Acp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336:SF0">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15955">
        <property type="entry name" value="Cuticle_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001562-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="13587" checksum="B67E812A6CB24BB7" modified="2013-10-16" version="1">MKVFRYRRSQLALAVAAHASLLAGLPYSYQSLTAHGAGAWGGLGAWGGPGAWGGPWGGHWGGPSVAIHTGPALGWGGHGAPAAALASLAHGPISLAQGPAGHEGQYVAKTRGAIHTAPLAGHINSATSLNLAPAPGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE12</accession>
    <name>T1GE12_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001569-RA">
        <property type="protein sequence ID" value="MESCA001569-PA"/>
        <property type="gene ID" value="MESCA001569"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1181390_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001569-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="235" mass="26355" checksum="B9D77341D0028B1B" modified="2013-10-16" version="1">MMGTRKITFTIGKHSSTMSVSRGTPQGGIFAQDDGENMNRARWSSSSALRLRNLKVWFEKSYGVRQLSLIPLYLGIPGREEWLNISTVQISYINIFTDNSTSSGGCGPGVAFPEMSIFNPFILPNQCTVFKILAILKAAETLLSLEIADKIDIDFLTRKDDIRRIFFNDMLVTCVSQSIPNFRACKSQNAVVSNPPLHNKISKNSFSQLSVLFYNYLTGNSFQTLLLKDLCLKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE24</accession>
    <name>T1GE24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE24"/>
    <dbReference type="STRING" id="36166.T1GE24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001581-RA">
        <property type="protein sequence ID" value="MESCA001581-PA"/>
        <property type="gene ID" value="MESCA001581"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067270_0_0_1"/>
    <dbReference type="OMA" id="ICHPAVH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13305" checksum="654D8C9D93E17348" modified="2013-10-16" version="1">MLVKVGWAWRLVDLQSIQRYLEHDTTCPSCRKAFDKGKRRKIIDTCGHERCYSCLFISDQCPKCINNSLKDVDGSNVQGYDTGIGGSTSTIVSPIGSPQPQVRSHIRTTNATVLPKFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEG0</accession>
    <name>T1GEG0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02073405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GEG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001722-RA">
        <property type="protein sequence ID" value="MESCA001722-PA"/>
        <property type="gene ID" value="MESCA001722"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006586_13_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147026" description="Carboxylic ester hydrolase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="19"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="187"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001722-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="563" mass="64343" checksum="D285AEC78038B2E3" modified="2013-10-16" version="1">MSRILIIVLFFPAIFANVPKVTLSHGGILVGNYTKTINGRQISAFKGIPYAQPPLGNLRFKSPQPFPAWQGERNAINESSVCFQVEAGIRGTTPAGQEDCLYLNVYVPTHVHRKKKPLPVMVWFHGGGWIYGSGGEEFGGPQYLLDKDIVLEILDLKIXXXXXXXXXXXXXXXXXXXXXXXXXXXQAEILKWINKHIASFGGNPNSVTIFGESSGAVSVMFLMHSAQCNKLFHRAIAQSGTEFNTFGHPHRKGKAAERAMQLARQVNCDKNEGNWKEIIDCLREVPGDTLIRQFHKYYEWEFFPTIPLSAVIEENHPDAFITMDPRISPVPQNIALIVGFTSEEGAIYSAPILNSETMLRQLKENYTNLMPLMLYYDHLEKSRQDEVTSKIIEYYLKNGHDYSPDNFENFTQIITDGMFFVGFDQLVRRRVSQSSPKTYAYVFNHRGAKSITSILGGGSRYYGVSHADELQYIFTWDKILNITESNFRDEAMRDAMVELWTNFVIHGDPTPRGCKYHNWKSVDGFLWNYARIGSNNTEERRMIWNERELVKDRIDFWNSLRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GET5</accession>
    <name>T1GET5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase UBR-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02059809">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GET5"/>
    <dbReference type="STRING" id="36166.T1GET5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001856-RA">
        <property type="protein sequence ID" value="MESCA001856-PA"/>
        <property type="gene ID" value="MESCA001856"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1612705_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012791142" description="E3 ubiquitin-protein ligase UBR-like C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase UBR-like C-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001856-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="19428" checksum="0B637260B086D03D" modified="2013-10-16" version="1">MLTQMIFMFPALAFASEDRAVVVNGSHLEFYLLKLMFMGCIVKGIVLFENVDEMDEDNLDEEMDVDDREIKSFQLISQFYSKYNLKYRSLECKKPITEKTVRKMFEFVKNSCMQFLRCSCLLFHFLTDVDFPEEMQVLEGEGINRGNLHVHLVHDNVEIGPLWIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEZ4</accession>
    <name>T1GEZ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001921-RA">
        <property type="protein sequence ID" value="MESCA001921-PA"/>
        <property type="gene ID" value="MESCA001921"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515231_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001921-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9694" checksum="36759C14673D4AEF" modified="2013-10-16" version="1">MQPRDPRDVIYYKHLGRPRIQQGRLCDGSIHIQKEGAVIDHVLLENDIGNCVIPKGPETEETPEEVEKAKKAYDFHEDNPGIPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF16</accession>
    <name>T1GF16_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196444">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF16"/>
    <dbReference type="STRING" id="36166.T1GF16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001944-RA">
        <property type="protein sequence ID" value="MESCA001944-PA"/>
        <property type="gene ID" value="MESCA001944"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005713_0_0_1"/>
    <dbReference type="OMA" id="IWYENTE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18500">
        <property type="entry name" value="BACK_IBtk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="148"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="525"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="661"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1002"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1002"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001944-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1104" mass="127315" checksum="2DCA8090654F80EE" modified="2013-10-16" version="1">MFTISNFDYNCTKKCNYRPHGNTITAALTKRAVNDQSLASFVAKSCRNFAEVLDSLGRSSLHICCSVDRYFLAEWLLNHGSFINYKDLESGSTPLHRAIYYGCIDCAILLLRYGANPEVMDVDKRTPFQYICKLQDFPEYRNKVPPLNELLVWGANKNYNLGIINDNVTTMPQSIDSFRKEHISVETFWPWESGRCGTGDEATLPSPKKIKNPIKNSDDKIIQVSCSKNHTLLLSAKNSVFSCGLNDKHQLGFKPAPEKQLSFREIQFPEGLSNAQKIIAKDNFSLLYTKKEIYVWGENCGQFGMPSSENIVLLPRRIDLPSKAQVELVECSNIAIAAVLTLKHVIHLFYKYKVTTIKVPNMEQVKSVQISGGGGDTSGGRGSAAGLRLIILTSTNVLFIWYENTEQFLRCSFSNIHLRQIDKMLCKSNQLFILSQGDVYTGKCSQITFEKVFDDDESSWFCKGFAKKEVSQNYRIKVELKQIPQIDRAESSRKYFNLPNLRSVQEWDNNYKKLLNDAHEFDEIHDVVFHVDSEVFYCHKFIIYSRSIGLRDIIEQQIDKNIYLRHLEGLTGKMFEIILKYIYSNYYFVIEDIDDIIQSLGSEFCGDRFDAGQLFLGILEMLELSELSHHLKVLFNIADTPSNPKKHNFPRLKRTSYSKLHDVIIKCEDNQQIEAHKCVLVSKMDYFNLMFTHSWSEKSVINLTTVSIEYLQPLIDFVYTLDAEYFKRQNYSESFLYQMIVIADQFFVESLKKVCEILILEKISVKKCGDMLEFAIIYNCEILKKGCLEFICHNLNRVLSQKSLEGCEPEVLKAVNKKYREIFKEVFDYRMITPNSDAVEDEVLESFVADFEVDLEYRMSEEDFLEADKDKKKEKELRVRETDTRQHEKHAIMSMRELNLRDERKVSESNDIKKQVPIERKSWTTNYEILKSESKPVDNFKELPQKKTVVDPQPSTSYAAMNLGDFGLHKGQKMSQKERKRLSSTSKVDVFETPVKTIPSPTVTSPWGVVSPSTSEASPISLDSRRSRKISTDASFSEILENERRQKEYIDKMASKSLHLTQIEELAIGELRQFYNVDNIGDEVITIQRKPMPTTINFAVWQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF31</accession>
    <name>T1GF31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="10">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GF31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001960-RA">
        <property type="protein sequence ID" value="MESCA001960-PA"/>
        <property type="gene ID" value="MESCA001960"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1311397_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="covalent" evidence="10">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001960-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23054" checksum="F7C56F929BE56A54" modified="2013-10-16" version="1">MAATLGRICGSQLLKNNSSKSLQQVSNFSTTKAWTNGQKKLLATVGATVGGAGALMFALESSVKASGTEVHPQTMPWSHSGLLDSLDHTSVRRGYEVYKQVCAACHSMRYIAYRNLVGVSHTEAEAKAEAEEIQVRDGPDDSGNYYMRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPKSISKRGSRSCCQQWSLSSRFELHRFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF63</accession>
    <name>T1GF63_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02117005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02117006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001994-RA">
        <property type="protein sequence ID" value="MESCA001994-PA"/>
        <property type="gene ID" value="MESCA001994"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375162_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001994-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10631" checksum="3FF773ECF86D4BDA" modified="2013-10-16" version="1">MEMTSSGSGSGWYFLIFNRVRRRALNSSLATKSHRSGEISPPCDVPLLTADITIAISEITILEILILFTHTTCESQYDSMSRKAVNEYSFLLKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF95</accession>
    <name>T1GF95_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox protein orthopedia</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02135501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF95"/>
    <dbReference type="STRING" id="36166.T1GF95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002030-RA">
        <property type="protein sequence ID" value="MESCA002030-PA"/>
        <property type="gene ID" value="MESCA002030"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125147_0_0_1"/>
    <dbReference type="OMA" id="SAGPKEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000719">
        <property type="entry name" value="Homeobox protein orthopedia-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051895">
        <property type="entry name" value="OTP_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46770">
        <property type="entry name" value="HOMEOBOX PROTEIN ORTHOPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46770:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN ORTHOPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="66"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002030-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="12597" checksum="D8338068C9092A70" modified="2013-10-16" version="1">PKDCDLPKSALNLHGGGSGGFGGAGQIGNNNNQQSETNGTGRLHVSGGLCEPPNMQDQNSDKSNKQKRHRTRFTPAQLNELERCFSKTHYPDIFMREEIAMRIGLTESRVQVSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFS8</accession>
    <name>T1GFS8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002228-RA">
        <property type="protein sequence ID" value="MESCA002228-PA"/>
        <property type="gene ID" value="MESCA002228"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778755_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7844" checksum="DDFC98D04C5B4D38" modified="2013-10-16" version="1">MKRSIMLVNCYNDTIPSADTACAIIWLRKLDSIIPDVELQSAYEGRVLQLIIAVVCMNREWCISTTIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFY4</accession>
    <name>T1GFY4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lin-52 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02140971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFY4"/>
    <dbReference type="STRING" id="36166.T1GFY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002286-RA">
        <property type="protein sequence ID" value="MESCA002286-PA"/>
        <property type="gene ID" value="MESCA002286"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226212_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070176">
        <property type="term" value="C:DRM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018737">
        <property type="entry name" value="DREAM_LIN52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31489">
        <property type="entry name" value="LIN52 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31489:SF2">
        <property type="entry name" value="PROTEIN LIN-52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10044">
        <property type="entry name" value="LIN52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002286-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12160" checksum="E01DBC3EAA287460" modified="2013-10-16" version="1">MKDLLACNLFIVFKVFINFFEFQVPGLNNFIKMSDTPIHSQSTATWARGLTQEEINSMHQLGNLSTNQLIKEITKVYDEAYKLGVIEAKEISRGKFLGIFQNTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGG7</accession>
    <name>T1GGG7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002487-RA">
        <property type="protein sequence ID" value="MESCA002487-PA"/>
        <property type="gene ID" value="MESCA002487"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1284598_0_0_1"/>
    <dbReference type="OMA" id="TENCATK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="25009" checksum="DF6579885AC2241C" modified="2013-10-16" version="1">MEIKDIHVGLATKRFLKSCGAKETEILKFFYNCKLVIILILKKIIVKSPIKFSFIRNAISLDPTYILSCENSSNEKMNKLLQELFEANAITENCATKAIRQYELFCSEEKEVLKKWKSERIRLDVFYGTNLKDKDDFEELWYVIRIVLTFFHGNADVESGFSINKELITPNQKSQSLVAIRRIKDFILNEGGLDQISITDDMLRSCRNSRTIYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH79</accession>
    <name>T1GH79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02195443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH79"/>
    <dbReference type="STRING" id="36166.T1GH79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002769-RA">
        <property type="protein sequence ID" value="MESCA002769-PA"/>
        <property type="gene ID" value="MESCA002769"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357260_0_0_1"/>
    <dbReference type="OMA" id="NVHIAKH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001864">
        <property type="entry name" value="zinc finger protein 883-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="453"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="55"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002769-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="785" mass="90405" checksum="B10DB59AEDD2D8F4" modified="2013-10-16" version="1">MNEFNQNYSNYDLFSTNSFQYQQQVDTNCYYAEHQNLVSSSVDQLPFVQIHTTHVEPSPENFHSSRIYRKPQSEIKSEEPIKSEQNDNHNDRVSRNSTPTTSTTPTVTNSSTSNKLPLSVFKCSRCPFLSIFQENLQKHEFQKHSSNESSNTALLTVVCSGCSNVFHSKISFDSHLQNDHEMEVNERNLLVTDLFPEITISEEQPRIPPKQKISIKSVDVLCEPQNRPKIYIKNVDILKKPLFDIPLIPNNSYIDNLWILCPFLMNQWVSLDASEILEQPQILEERRATNYIHLRTVDEVNLMNRDDVDNLMAPTDDGLWSHFEDLGNEIQEISEEPQKETHYDLNTPEVLLAPCEPAQTDDDPEILFVCAEEIINANPPVSDTNFVLEEPVEFPEETTFVEDETQEVPEKGRIYVSENLMAEKSSKKINRPRAINNFSKMKKMFGTTIEETVKCDVAGCGFRFKNPETLDYHKKCHSDKLNKIICPECNSEDLSNWNTLHTHLWRTHSIDMELYSCHLCKFKTPIYSRLTNTHMKIHSEERNYQCDQCVKAFKNSKQLKNHRRLHRTNNFTTQIFKCEACRSAFSHLKSLKAHSCKSGDDSKVKEVKCTICDKILSSKSACKLHMLTHESASKFKCENCSYVTNDHNAFRRHKLQHDGINGKMYECQFCDARIFRAPVFRLVLKHIQQQHPEHADKIILKCTSCPFTTISKNLLNVHIAKHSGNVGGNSEDAAYEIEDETQETEQPQRNKIKVKSDLVLTDPSKFQNGAMNSSILLHNLNEGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHE6</accession>
    <name>T1GHE6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase ARIH1-like UBA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHE6"/>
    <dbReference type="STRING" id="36166.T1GHE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002842-RA">
        <property type="protein sequence ID" value="MESCA002842-PA"/>
        <property type="gene ID" value="MESCA002842"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1820623_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase ARIH1-like UBA-like" evidence="2">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002842-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16382" checksum="EB02538B4D98EAE7" modified="2013-10-16" version="1">MDSDEESFGKSDSGHNSPTDDEDDFPMEMDQPPVKEKAMETDEYPYETLTTESIVQTMIDCIDDVNSVLNLPKTTTRILLNHFKWDKEKLLERYFESNQEKFFRDAHVVNPFNKPKLKSKPKVPSSGLEECEICLSTFPPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHI5</accession>
    <name>T1GHI5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02100685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHI5"/>
    <dbReference type="STRING" id="36166.T1GHI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002887-RA">
        <property type="protein sequence ID" value="MESCA002887-PA"/>
        <property type="gene ID" value="MESCA002887"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624822_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002887-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9033" checksum="2E8F92589BCF0B24" modified="2013-10-16" version="1">MSYESHHSSHHQTGISTDKMLESLSLQLREAEMRKQEAERGHQDAISQLRMLNASGSVAKNSEQIENLQLEQENWKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHP4</accession>
    <name>T1GHP4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002952-RA">
        <property type="protein sequence ID" value="MESCA002952-PA"/>
        <property type="gene ID" value="MESCA002952"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2099669_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002952-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13426" checksum="A31B103AF0D46C4B" modified="2013-10-16" version="1">MGTEARGMEDNKLDVKAENEIASAIKKKAGKLESWRPLRNRRLVFTQLRCKTVPKALEEMMLIDRVFLDHLKDLSERVELIGRTLSKNETSNAKYMQFQASSQEECEDLVRNVEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI57</accession>
    <name>T1GI57_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI57"/>
    <dbReference type="STRING" id="36166.T1GI57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003125-RA">
        <property type="protein sequence ID" value="MESCA003125-PA"/>
        <property type="gene ID" value="MESCA003125"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369283_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016533">
        <property type="term" value="C:protein kinase 5 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004944">
        <property type="entry name" value="CDK5_activator"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03261">
        <property type="entry name" value="CDK5_activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003125-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="21863" checksum="007366BAC589BD39" modified="2013-10-16" version="1">HQHHNHHHHHHSSNSSSSSSCRGRTSIGGGGIGIGEGGGRRTSGGDVGSVHSSCSISSSNSADFQLNNYSYEQLNNVKNRENSKPPNTVNNNNNNNNNNLNNNINNNLSSSNTTNTTNLDRTNNNDTARILSEKNALEKNLKKHSLFINALSWKRLASSHGKKKLESNQKNKAANLPTATFRPPLADAIHPLQYLSLMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIB5</accession>
    <name>T1GIB5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIB5"/>
    <dbReference type="STRING" id="36166.T1GIB5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003185-RA">
        <property type="protein sequence ID" value="MESCA003185-PA"/>
        <property type="gene ID" value="MESCA003185"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1614993_0_0_1"/>
    <dbReference type="OMA" id="HASHTVY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="1">
        <location>
            <begin position="73"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003185-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="18433" checksum="BCDF29B040E1DB58" modified="2013-10-16" version="1">METHLRDECTALAIACSFKEAGCRFKGPRHLLETHLEANAASHLSLMVALSSRQNQQIQMLKTAIDKLSTNCTGTLLWKISDWSMKMAEAKNKDGLELISPPFYTSQYGYKLQASMFLNGNGPGENTHVSVYIKVLPGEYDSLLKWPFSHSITFTLFEQGSQNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ16</accession>
    <name>T1GJ16_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ16"/>
    <dbReference type="STRING" id="36166.T1GJ16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003455-RA">
        <property type="protein sequence ID" value="MESCA003455-PA"/>
        <property type="gene ID" value="MESCA003455"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_1_2_1"/>
    <dbReference type="OMA" id="VYETSHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001234">
        <property type="entry name" value="Serine/threonine-protein kinase par-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF93">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="331" mass="37655" checksum="93543624B8229522" modified="2013-10-16" version="1">MYLIMEYCVGGLQELIDSAPEKKLPLFQAHRYFKQLIYGLEYLHGRGVIHKDIKPGNLLLTLDQTLKISDFGVAEALENFAIDDTCSTAQGSPAFQPPEIANGLEVFAGFKVDIWSSGVTLYNMCTGKYPFEGDNLYKLLENIGKLNWREPPEHLNNVDKDLADLILGMLEAAPCKRLTLQEIRSKIWFIKTPEKRGTEIPIPPLKGDQHRCSTVLPFWNHITMKWNKIPKILANQSNNNSQNINEKNEVNTKTEFDPNSSSSSKKSKKLDTSSSSNKRRSKKPVSCISVRKLTHCRTSYPNNNIWSYNDFLEKHPRFEEKKSTCSSIVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ24</accession>
    <name>T1GJ24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ24"/>
    <dbReference type="STRING" id="36166.T1GJ24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003463-RA">
        <property type="protein sequence ID" value="MESCA003463-PA"/>
        <property type="gene ID" value="MESCA003463"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253115_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF40">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003463-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11826" checksum="E8DB5BAB4B917B79" modified="2013-10-16" version="1">MSYVINIEERDRYRHAETAHNRIASVVVGFKEALLEEAEKKKDTRNWRIILQRISVNILVIGLLAASGYTVVSAVGSKEIVTYGSFIQRIFCSKSNAKKISRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ55</accession>
    <name>T1GJ55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ55"/>
    <dbReference type="STRING" id="36166.T1GJ55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003497-RA">
        <property type="protein sequence ID" value="MESCA003497-PA"/>
        <property type="gene ID" value="MESCA003497"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2365575_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003497-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11297" checksum="EBEE199032DA3901" modified="2013-10-16" version="1">GDPNFQRKTLVLLGAHGVGRRHIKNTFIAKYPDKYAYPIPRSGHRSTLSNFCFWRGNLSWNRLEKAQNLVQDPSMRNIYYSYREISHGTAWKKLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJL2</accession>
    <name>T1GJL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJL2"/>
    <dbReference type="STRING" id="36166.T1GJL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003661-RA">
        <property type="protein sequence ID" value="MESCA003661-PA"/>
        <property type="gene ID" value="MESCA003661"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2352592_0_0_1"/>
    <dbReference type="OMA" id="ECTNHED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10472" checksum="9DE9308344D0E54B" modified="2013-10-16" version="1">MQPLKKFTQCSGLTLLFGVFIILFGVSSTALTTFGKSNIPSSTQAPAIKSRECTNHEDCNSLKDTSCVKDPDDYKLRCLCGDDTAPDNGFCKKTAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJV1</accession>
    <name>T1GJV1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJV1"/>
    <dbReference type="STRING" id="36166.T1GJV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003755-RA">
        <property type="protein sequence ID" value="MESCA003755-PA"/>
        <property type="gene ID" value="MESCA003755"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_5_5_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="2">
        <location>
            <begin position="2"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003755-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="46" mass="5200" checksum="17F0FCB93E20231B" modified="2013-10-16" version="1">MALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJV4</accession>
    <name>T1GJV4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02392220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJV4"/>
    <dbReference type="STRING" id="36166.T1GJV4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003759-RA">
        <property type="protein sequence ID" value="MESCA003759-PA"/>
        <property type="gene ID" value="MESCA003759"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_4_0_1"/>
    <dbReference type="OMA" id="NYIWLLQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000019">
        <property type="entry name" value="Hydroxyacylglutathione hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="11"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003759-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="257" mass="28623" checksum="0F7E2551C46FB310" modified="2013-10-16" version="1">MLVKILPALQDNYMYLVICPETKIAAIVDPVEPETVLAAVDKENVKLNGVLTTHHHWDHAGGNEKLLKLLKDPIPVYGSNDERIGGLSNVVSQGDVIKIGNLNITCIFTPCHTTNHICYFVESPNGDKCVFTGDTLFQGGCGRFFEGTADQMYSALIEKLSSLPDDTKVFCGHEYTLQNLAFAKNVEPNNELITKRIQWAEEKRHQNTPTVPSTIADEKSWNPFMRVHIPSVQAHAGECDPIKTMASLRKEKDSFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK09</accession>
    <name>T1GK09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02033103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GK09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003820-RA">
        <property type="protein sequence ID" value="MESCA003820-PA"/>
        <property type="gene ID" value="MESCA003820"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_558507_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="78"/>
            <end position="166"/>
        </location>
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            <begin position="1"/>
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            <begin position="1"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51293"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003820-PA"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="55468" checksum="25290FCA85787C6D" modified="2013-10-16" version="1">SPQNQSNEQIPDTSSETEKEDSVGPVSSTTQDIPCKESENPSLLATIAVEEDVEDEEDDGDYSPEDETVDNQDDDDTKKVKIGCEYQANIPDGLCIYDDVLPYNNEDKLVWCPFAIDDKSIENYLDNYREIDKIQQQQLENEKNLKEKEKNSGENSSEDKNLPTSTQKTSLNLFPFSSPMSSWSEEECRSFENGMRLNGKNFYIIAKTKTTTRSVSEICEFYYNWKKTTRYDAFVSKQNGNGSGEDKKSVNNFRKSNASVTDYMDKFLDEDSSASDNMLNGGDGSEKVFNLRKRRGSSVSSASSDNNNGHLKRSEDDEMSLYSAAATVANSDALHFPPDLDESEDFENATSIQDFESEKTIAGDQHEIPKKYSLPPSTSSEKNKNRKRKPSEEGSEDVEFEPTIEMMVHDFDDEKTIEEEEKRAMEENESYQHEVESLQKESEMPLEELMKLYAAPPPLEIPSKSRKKKSSKGDSXIIKILNNEHFKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK16</accession>
    <name>T1GK16_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gem-associated protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003827-RA">
        <property type="protein sequence ID" value="MESCA003827-PA"/>
        <property type="gene ID" value="MESCA003827"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798662_0_0_1"/>
    <dbReference type="OMA" id="NDHESID"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003827-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="17007" checksum="C018270FAE66BE72" modified="2013-10-16" version="1">MPPNLNNGIIESKKKKRKRKKNNKKKSDSSQDQSLAWRKTFENLTQWHSNHQVLYWKGVAERYQQQNQKLLQKLQSLEESNDDDESSDSEEDSDPTIIPLTKNDHESIDIDEEYLNFLEITRKHREEIQLQKLKDAASEPDTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK54</accession>
    <name>T1GK54_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-Cys peroxiredoxin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [protein]-dithiol = [protein]-disulfide + an alcohol + H2O</text>
            <dbReference type="Rhea" id="RHEA:10008"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK54"/>
    <dbReference type="STRING" id="36166.T1GK54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003870-RA">
        <property type="protein sequence ID" value="MESCA003870-PA"/>
        <property type="gene ID" value="MESCA003870"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF3">
        <property type="entry name" value="DI01187P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="10">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003870-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="27360" checksum="1867E23E33F6063C" modified="2013-10-16" version="1">MLSYSPVFQVDEENVASTKNSMRLGATVPNSRRKPQMDQLSSTIGKEIREWVLIFSHPADFTPVCTTEIGNIAGFTEEFKKRNTKIIAHSVDRLKTHHDWIEDIKSYCSRVPEKFPFPIIADTTRELAVQFDMIAEEDRHNEDTAQSVRALFIIAPDHKVRLSMYYPNSAGRNLQEIIRVLDSLQLTEKLKVVATPANWTPGTKVMILPSVTDAEAEKLFPGGVDKVSMPSGGNYIRTTEMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKL5</accession>
    <name>T1GKL5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034444">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004041-RA">
        <property type="protein sequence ID" value="MESCA004041-PA"/>
        <property type="gene ID" value="MESCA004041"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906695_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007970">
        <property type="entry name" value="DUF733"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05306">
        <property type="entry name" value="DUF733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004041-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7686" checksum="2ACCACB634BA9D29" modified="2013-10-16" version="1">MVEETVETMSYILFVYQEELRRKKARVIRLSKQKLQLTEKLVMRNSNIVIRIWHRPNEFYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL18</accession>
    <name>T1GL18_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIB43A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02200225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL18"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004204-RA">
        <property type="protein sequence ID" value="MESCA004204-PA"/>
        <property type="gene ID" value="MESCA004204"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485933_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008805">
        <property type="entry name" value="RIB43A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517:SF6">
        <property type="entry name" value="RE41410P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517">
        <property type="entry name" value="RIB43A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05914">
        <property type="entry name" value="RIB43A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="31"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004204-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10766" checksum="6D7A1DADCA46D7C3" modified="2013-10-16" version="1">MHFFQQRKTQEFIKEREWEEQQNNNARILILKQLEMDKLKKEKLMENKSENLELAKEQNDVKTYMNKKVYVNEATNEFYDKFNTTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL19</accession>
    <name>T1GL19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02200223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL19"/>
    <dbReference type="STRING" id="36166.T1GL19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004205-RA">
        <property type="protein sequence ID" value="MESCA004205-PA"/>
        <property type="gene ID" value="MESCA004205"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2164701_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051875">
        <property type="entry name" value="Calcineurin_B_homologous"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46002">
        <property type="entry name" value="EG:114D9.1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12530" checksum="5D8E67D79452F355" modified="2013-10-16" version="1">MPGFSSYSQTNSEIKLSRIMKCSHVPRTDSIRSIVRSSVISFFDSALAFQMYDLDDDGVISREELLNILHMMVGANISQDQLMSIAERTILEADQRCQGKISFDDFCNALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL69</accession>
    <name>T1GL69_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL69"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004262-RA">
        <property type="protein sequence ID" value="MESCA004262-PA"/>
        <property type="gene ID" value="MESCA004262"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471636_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004262-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10262" checksum="3CF33AB07FDB9842" modified="2013-10-16" version="1">MLQRLSVQNSTGNPVPSTNNDFYHQTAHLAIRHQKFYGNNPLRFAPFLPTFDQRRFPLFGDFYLQQPQPFPYNPQGFHGNFLPPPTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLT9</accession>
    <name>T1GLT9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Torsin-1A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLT9"/>
    <dbReference type="STRING" id="36166.T1GLT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004503-RA">
        <property type="protein sequence ID" value="MESCA004503-PA"/>
        <property type="gene ID" value="MESCA004503"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124512_0_0_1"/>
    <dbReference type="OMA" id="QNIWRIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049337">
        <property type="entry name" value="TOR1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760:SF2">
        <property type="entry name" value="LD13476P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21376">
        <property type="entry name" value="TOR1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Torsin-1A C-terminal" evidence="1">
        <location>
            <begin position="97"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17926" checksum="B39E6C2259E0057A" modified="2013-10-16" version="1">MPSGIFESIASLLDHHSSLNGYDFSRAIFIFLSNTGGVEISETLTQLTKNGKWRKDTKLYDFEKILEIGSYNIDGGLKETSLIESHLVDHFVPFLPLERSHVRLCLQKEFEKWGSFPTEDIITEIIDNFVTFDKQTGMYATSGCKTLEKKVAIYVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLU3</accession>
    <name>T1GLU3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLU3"/>
    <dbReference type="STRING" id="36166.T1GLU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004507-RA">
        <property type="protein sequence ID" value="MESCA004507-PA"/>
        <property type="gene ID" value="MESCA004507"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017361_0_0_1"/>
    <dbReference type="OMA" id="ETAYHAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="7"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004507-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="259" mass="29373" checksum="8B3ABA4F3F935F39" modified="2013-10-16" version="1">MICEKQFVKSIEEWRREMKIPQMILLGHSMGGFLASSYALSYPDHVKHLILADPWGFPERPNETSNKTLPLWVRAIAYALTPLNPLWALRAAGPFGQLIVEKTRPDITRKFSVITKDENSNLIPQYIHQCNAQTPSGESAFHSMMESFGWAKNPMINRIHEVRKDIPMTFIYGSRSWIDSSSGDKIKTVRADSNVSIRIVSGAGHHVYADKADVFNKYVNEACSLTDSNSIQLKTTNFPKISNDTEEEELQDEKDKKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLU8</accession>
    <name>T1GLU8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004512-RA">
        <property type="protein sequence ID" value="MESCA004512-PA"/>
        <property type="gene ID" value="MESCA004512"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323031_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11012" checksum="6C14EBDE21BFF178" modified="2013-10-16" version="1">MQFAELEEQDLKAIAGSSWGKDKETIQNIFQTHVSKGGSSSRGRIVVRFVLNLPFSCPSDFLKRGDVHDIFLQYFPYILCGSLSSHAPGVPSFNLHLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW2</accession>
    <name>T1GLW2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004527-RA">
        <property type="protein sequence ID" value="MESCA004527-PA"/>
        <property type="gene ID" value="MESCA004527"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239676_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004527-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12346" checksum="319A7BE4C8F7B2B5" modified="2013-10-16" version="1">MMSRDNAISFLKEIHKGMNYLEKSYDRKMLKNLMQNHMKKNMLFPSTTTAPALSVLSENLNVNLENQNKYFNQNHLLDKNQELYTVKTGERDCQFQNDCISLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW7</accession>
    <name>T1GLW7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02101686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLW7"/>
    <dbReference type="STRING" id="36166.T1GLW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004532-RA">
        <property type="protein sequence ID" value="MESCA004532-PA"/>
        <property type="gene ID" value="MESCA004532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_42_11_1"/>
    <dbReference type="OMA" id="QSMGCSS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16372" checksum="FD674B12C3CE1563" modified="2013-10-16" version="1">MIKAGVETNLEVYTSENISRKDGKLFQCEKCDKIFSTKTNLNRHFATHHEGRKFQCKICEKLFTQSGSLKQHMYIHTGERPFGCVVCGRTFTQAKTLQLHLRRHTGEKPFRCDHCGVFFRQKDGLKRHVSVKHEMASNENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM41</accession>
    <name>T1GM41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inward rectifier potassium channel C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02053054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM41"/>
    <dbReference type="STRING" id="36166.T1GM41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004609-RA">
        <property type="protein sequence ID" value="MESCA004609-PA"/>
        <property type="gene ID" value="MESCA004609"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022738_3_1_1"/>
    <dbReference type="OMA" id="LPMHRST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF115">
        <property type="entry name" value="INWARDLY RECTIFYING POTASSIUM CHANNEL 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 11" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="14">
        <location>
            <begin position="236"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004609-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="394" mass="45834" checksum="962EB9CEF6CD7810" modified="2013-10-16" version="1">METDTTTAGMHRSVSMTDTPKTINPRTLVRSDEFETLDEIPSESEMSLDHEFFSKDINAKLDIQKKNHKENWAGSTIELNVRKHSTKSARQFDRTYMKSRKSIQLKSGRVIQKNGVENVNNLSMKGRSWKFFKDIVTTLIEWDWKYMLLLFFTTYIGSWFIFSLIWYLIGYAHGDIPYNVPSKAKGHHSREPCLKGAYDLTSMFLFSFELQTTIGTGEKYPTDECPEGVFIFILQVCYRNNKLCLMFRVSDPQEQHSIELKVRAYLLIDKITNEGEVVKVNTELKLLGNGSNFIMWPETLIHVIDCDSPFYNFTAEDFHSENYEVFVSIFGSSPLTAQVSEDRTSYLPKEVFWGQRFNNIIHYDKLNNSYFVDFKNFNSTSSVSMEKLEDRSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM97</accession>
    <name>T1GM97_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096752">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM97"/>
    <dbReference type="STRING" id="36166.T1GM97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004666-RA">
        <property type="protein sequence ID" value="MESCA004666-PA"/>
        <property type="gene ID" value="MESCA004666"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511217_0_0_1"/>
    <dbReference type="OMA" id="CANFTIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052752">
        <property type="entry name" value="NACHT-WD_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF37">
        <property type="entry name" value="GH25853P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004666-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="533" mass="60141" checksum="4CDBDC50D9631727" modified="2013-10-16" version="1">MKQKFIFTGNNILEMGNVCSSSKKKNKDTISERSEESPSYQLSSKRNSKANKDQINDKLAPLADTELSADLINQSKNGTAGKTSISKSNIPDSVTSTPQAMTAPEELTSKTSLPHNLIPLQGRPRSSRRTEKDKKIILYILSSGYEHNIEKAVLSDLYEILKERCDVRGFELIYSDNHSQFETAEDKHQILDILQWKTYPLEAQGGHDEAANCLADVVRHTNASYMVPILFLGTSLGRPLLPLTIESQDFSTVYSSATEKLQTLLNKWYKLDDTELKDSYLTTIVEQEIHNTVLISQELAKRCMWVQTGIFPIKVSENVGLVEMENCQRLMNLHSELKNQLPEKNLIRIPPSIQVKDDQLSSIMESILKSILDGIFDEHTQKYSIPNCTFGVNKKLLSEIETTGQYSKMLGQNCANFTILEDIKRYVMEDSSAPLIILGEKGSGKSVLTSKIMNYIHVWSPEFNLILRYSHLTEMSSNITSLMGSIADQFSILLKGSISECKHSIITILYIILSVIRLCICISYIFQPKSHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMP3</accession>
    <name>T1GMP3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004821-RA">
        <property type="protein sequence ID" value="MESCA004821-PA"/>
        <property type="gene ID" value="MESCA004821"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725100_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004821-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8529" checksum="DC5FC12602F68E4E" modified="2013-10-16" version="1">MLSQKAGPLYKRRPTMKFKYTSQNSTEWEDSIIFDSDDPDFVRSTEELYRTIVPSWNSIRPRTSSFTLRATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN43</accession>
    <name>T1GN43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein MMS22-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN43"/>
    <dbReference type="STRING" id="36166.T1GN43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004984-RA">
        <property type="protein sequence ID" value="MESCA004984-PA"/>
        <property type="gene ID" value="MESCA004984"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_691321_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042320">
        <property type="entry name" value="MMS22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029425">
        <property type="entry name" value="MMS22L_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28547">
        <property type="entry name" value="PROTEIN MMS22-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28547:SF1">
        <property type="entry name" value="PROTEIN MMS22-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14910">
        <property type="entry name" value="MMS22L_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein MMS22-like N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004984-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="399" mass="46839" checksum="0B4BF5E12A32B0AC" modified="2013-10-16" version="1">MQIIQLQETIKYGYVNTDEFSVFLDHAKSFIKDLIFISFRNFQNVADRDLYNKSPFPCACVKECWLAIQIMFDKLNINFWRICNEIFATYKTKINENNFTDIKFSLFKLWMIESLVRLEKFKIKEFKTNSVTSVIENSQFLKETVDLLGRYSSLEEDCRVFLKLWEPKTEIPTILWEHFYKKLNSTFMAIETMSKDLKVINASVEDYLEKLDKRVHFSEEDILNRKEELISNKNSFVIFTMIIGKTVQKLVQANEMHQINKLIGRIFVKFSGPKLFNLFESGLHNLIELFLICAIASEEPDIITKFRDKLLLANLTQVKTTKQLSIVRGHIAFMILSCRHMLNISDYLKKFMEQFNKVIDSTDICRLLASGLGDIFHENLHLDLGRSVLIVFEFVLSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN73</accession>
    <name>T1GN73_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005017-RA">
        <property type="protein sequence ID" value="MESCA005017-PA"/>
        <property type="gene ID" value="MESCA005017"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1772752_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005017-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16995" checksum="DE838A7571000DAA" modified="2013-10-16" version="1">GVDISDFEYNFPKNYELLRIVSVFSWELPVVGVYGGKNEWNYYFGNYNSSKNFAIYRNHSDWYSSTNPCDFVMGKEIFSKKPYVPTEIVLGRDGRFSVFVYGEEGFVLSCENSEELTDPVKIDIGYAGKKNYDSSVHKFFFDCPVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNC7</accession>
    <name>T1GNC7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005074-RA">
        <property type="protein sequence ID" value="MESCA005074-PA"/>
        <property type="gene ID" value="MESCA005074"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491990_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="3"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005074-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="554" mass="65060" checksum="B804C9149219F0F6" modified="2013-10-16" version="1">MDPKNLNDFPVELLVRIFEYQSSNIELRKISMVCKKWRDIVEEFFKYSEKIFLSSRLLSIYIEHVDPKNNLLTDQKKSLIRNYKCLSLPGEHYESVEPYIKTLDLKFLRISKVPFSKVLNLLEIYKKTLETLDFQFLSQDSGCCFFKLEKLKTIKVKIISQNDLNFLKSLLEHNSNSITSLELIINSEIDGDLEFLTKLPNLRTFQFHSKIDLNYSVGVGTIILYLLRRLQSLETLILYNVDENDLIKTSYEEAKEEQAHGFFYTDEVLSKEIPYLTKFSCLIYSRMAFRATVLQPHLTELKVFEFIYGDEVNAEFLKEIFKGWTKLNTLYLSVPGISLQEESTCIVFPTLTKLTLYNFVNISKFFRVVRAPKLLHLKIDSNMPYDYEFNGEEFYNLQHNIDMLDNLKTLDINEFMFYGSFPNAYFLKMEMGVYEDVLLFLKGLQSNSLNFQLVLYDGNDIERIEAESKIMKLFHEIKDELNLTMYSSYSKTSVVKCILSLYTGGAVHDLRRDLSEYKSDITALKEIRWTGEGELEDKSKYKCEIYFNCHPSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP10</accession>
    <name>T1GP10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005324-RA">
        <property type="protein sequence ID" value="MESCA005324-PA"/>
        <property type="gene ID" value="MESCA005324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500505_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9607" checksum="F3B6F11E3E90EF30" modified="2013-10-16" version="1">MFSATLEGTKRSETCAVKPEFMLLVAERIGAYAKGVFCHRQQHPSLCPFSLQLSSVLPTAINKINCRFRVNGAKRILPIHGARVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPE6</accession>
    <name>T1GPE6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ca2+-activated K+ channel Slowpoke-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02054096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPE6"/>
    <dbReference type="STRING" id="36166.T1GPE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005474-RA">
        <property type="protein sequence ID" value="MESCA005474-PA"/>
        <property type="gene ID" value="MESCA005474"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1596383_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ca2+-activated K+ channel Slowpoke-like C-terminal" evidence="12">
        <location>
            <begin position="62"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005474-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="18102" checksum="088BA1A97912466C" modified="2013-10-16" version="1">MFSNKIKDYCRQVNIVDEHHPAPTFTPPELPKKVHVRGSASELKSPKTSFMSNARMVEYALSSCSCRVGQISLYDGPLAQFGECGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVFVLMQFDPGLEYKPPAVRAPIGGHSANSQGTGVGGGGSNKDENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPR0</accession>
    <name>T1GPR0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02100806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02100807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPR0"/>
    <dbReference type="STRING" id="36166.T1GPR0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005601-RA">
        <property type="protein sequence ID" value="MESCA005601-PA"/>
        <property type="gene ID" value="MESCA005601"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512521_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF69">
        <property type="entry name" value="TENEURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tox-GHH" evidence="4">
        <location>
            <begin position="214"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Teneurin-like YD-shell" evidence="5">
        <location>
            <begin position="274"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005601-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="532" mass="59880" checksum="5AD558ABC22B6205" modified="2013-10-16" version="1">MEKLGYNVKNIVPQFSKQLWEQPSLWGRALESPLSLNLKKPINNSPTMAVESGFLAHLGLRRLSNFEDLSAPPKSALKFDVMNPKPTKIGSDTEPPFGKGIVVSRTSEGQAIVSSVPAANAIYRDVYTSVFNRSQLLPFTFVVHNAQQDSFFFIKEELWRASEDRQQLKRLQGQVNTTYHEIERENGSGSSYLDVKIHGQHAIINLRYGTTVEKEKQRLMHHAKLTAVRKAWHREKEILRNGLTSGIEWTQQEIDEILKQGYANNYEGDPDENGEFHIPYPPSSEIYVFNRYGQHVATKDLTSGKTRYSFLYSKNTSFGRLSTVTDSSGNKIQFLRDYSNVVSSIENTQDRKSEISINGIGIMTKLAEKGKYAIELDYDSNTGLLMSRSSGGETCIYQYDEFGRVTGMILPSGETVRITSQLADNKGLSVYVHASIESLFSQERKRQENAAAEIKPNNTLVIHDSNGVVVESSAIARHPLLEAALPVEAEMLPMWSHQSITMGDGLVNNMYSVYNLVGDVRNPQQTLNREIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR91</accession>
    <name>T1GR91_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR91"/>
    <dbReference type="STRING" id="36166.T1GR91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006169-RA">
        <property type="protein sequence ID" value="MESCA006169-PA"/>
        <property type="gene ID" value="MESCA006169"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712022_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006169-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8620" checksum="956F0782F00707F5" modified="2013-10-16" version="1">MYINFLQYRKMTNLKFNITLILFIFISSSLDFTLADIFTDYFSDYECNKPLMEYAVLTATSSLRDRGPEKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRI0</accession>
    <name>T1GRI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRI0"/>
    <dbReference type="STRING" id="36166.T1GRI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006265-RA">
        <property type="protein sequence ID" value="MESCA006265-PA"/>
        <property type="gene ID" value="MESCA006265"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1171795_0_0_1"/>
    <dbReference type="OMA" id="FERETYT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF328">
        <property type="entry name" value="CADHERIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="121"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006265-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="237" mass="26295" checksum="FFE91F199CB08A41" modified="2013-10-16" version="1">MGVTITLKDVNDQSPMFTSSNETSVMENIPVNTVVMAVKAIDRDDGRNGYIEYILKSEPMVPFTLGSVDGLLRVSGKLDRESRSHYTLAVIARDRGDPPRSSQTKIFVKILDENDNSPMFDPKQYSASIAENASIGAMVLQVSATDIDEGANGRIRFSIAYGDDNRDFSISEDTGVVRVAKNLNYERKNRYILKIQAEDCAADLWSEGSNSLPIDTKLDMAELTITITELTIIHRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS76</accession>
    <name>T1GS76_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02135201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02135202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS76"/>
    <dbReference type="STRING" id="36166.T1GS76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006525-RA">
        <property type="protein sequence ID" value="MESCA006525-PA"/>
        <property type="gene ID" value="MESCA006525"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725093_0_0_1"/>
    <dbReference type="OMA" id="KITLVCI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="2">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006525-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8129" checksum="15DD7474E04DF5C3" modified="2013-10-16" version="1">MKITLVCIISAIETATKLKKEDEVGDVTRDWKFAAMVVDRLCLIIFTLFTIIATLAVLFSAPHFIKNPMKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSM7</accession>
    <name>T1GSM7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006687-RA">
        <property type="protein sequence ID" value="MESCA006687-PA"/>
        <property type="gene ID" value="MESCA006687"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815380_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006687-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7724" checksum="EDFA6BE580EC4432" modified="2013-10-16" version="1">MTVSTTTTTQILYLCPKEPIYDRNQMILDTSAPRNRSMTVLNPNLCTLNKRKKIVRIGVFVEQKLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSX6</accession>
    <name>T1GSX6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Armadillo segment polarity protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02101881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSX6"/>
    <dbReference type="STRING" id="36166.T1GSX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006790-RA">
        <property type="protein sequence ID" value="MESCA006790-PA"/>
        <property type="gene ID" value="MESCA006790"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008757_1_1_1"/>
    <dbReference type="OMA" id="SHHQQGL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007367">
        <property type="term" value="P:segment polarity determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000015">
        <property type="entry name" value="Catenin beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013284">
        <property type="entry name" value="Beta-catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45976">
        <property type="entry name" value="ARMADILLO SEGMENT POLARITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01869">
        <property type="entry name" value="BCATNINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0709">Segmentation polarity protein</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="203"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="268"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="310"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="391"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="433"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="480"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="575"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006790-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="786" mass="86732" checksum="2CC6AC9496D717C7" modified="2013-10-16" version="1">MSYQMPQQNRNIVHNQHNSYNPADLPMPSAKEQTMMWQQNSYLCDSGIQSGVVTQVPSLNGREDEEMDGEQLMFDLDQGFSQNITQDQIDDMNQQLSQTRSQRIRAAMFPETLDEGTEIPSTQFDPQQQTAVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELIKLLNDEDQVIVSQAATMVLQLSKKEASRHAIMNSPQVVAALVRAISKSNDLETTKGAVGTLHYLSHHRQGLLAIFKSGAITTLHNLLLHQDGSKMQVRLAGGLQKMVTLLQRNNVKFLAVVTDCLQILAYANQESKLIILASGGPNELVRIMRSYDYEKLLWSTSRVLKVLSVCSSNKPAIVECGGMQALANHLVSQSNRLVQNCLWTLRNLSDAATKVEGLEQLLSSLVQVLASTDVNVVTCAAGILSNLTCNNQRNKITVCQVGGVDALVRTIIGAGDREEITEPAVCALRHLTSRHTEAEHAQNSIRMNNGLWVIVKLLHPPSRWPLIKAVIGLIRNLALCSENHAPLREHGAILHLVRLLSKAYQDIERQRSSVATTGSQQACAYADGYLAKESHNRVLIRQQNVIPIFIRLLYNEIENIQRVAAGVLCELATDKEASEIIEQEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSIELTNSLSLRDENNLWANNDMGMGPDFQDMLVPDAYEGLYAQGPPSVHSSHGGRPFQQGYDALPIDSMQCLDINSPMAGAGPSCAPTSPYSMDMDVPEMGGAGELNFNLDNMPTPPTDNTNLAAWYDTDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSY9</accession>
    <name>T1GSY9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSY9"/>
    <dbReference type="STRING" id="36166.T1GSY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006804-RA">
        <property type="protein sequence ID" value="MESCA006804-PA"/>
        <property type="gene ID" value="MESCA006804"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500473_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006804-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9569" checksum="DD690915CE3B1D9B" modified="2013-10-16" version="1">MGANPITALFKCVTEKRKKSGEKNSLKIEPAKIDDTFSSTTSMEEKGGATTKDLDQWIEQLNECKQLAEPQVKTLCDKQILIVLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTN0</accession>
    <name>T1GTN0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077445">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007068-RA">
        <property type="protein sequence ID" value="MESCA007068-PA"/>
        <property type="gene ID" value="MESCA007068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148241_0_0_1"/>
    <dbReference type="OMA" id="HAISPWI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031874">
        <property type="entry name" value="Cuticle_Acp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336:SF0">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15955">
        <property type="entry name" value="Cuticle_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577125" description="Cuticle protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="13407" checksum="10D49DCDE2DC357E" modified="2013-10-16" version="1">MVFKAIALIVLIGLTQGSVIPLSVVSPSLQYTIRGPAHIDAPVVLSHAISPWIVPHVIAAHPPAAVEVAAAPAPVIEVAAAPPKVVVAAPVVEVAEPEGSYVAKTRGSEHVAPLPGHSNSVTVLNAEPAPGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU49</accession>
    <name>T1GU49_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU49"/>
    <dbReference type="STRING" id="36166.T1GU49"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007256-RA">
        <property type="protein sequence ID" value="MESCA007256-PA"/>
        <property type="gene ID" value="MESCA007256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270475_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11278" checksum="DBA695C72D421548" modified="2013-10-16" version="1">METDKMVKAMARWWQTVKEDDHDAGARNPMLYDPLTENNVKTTKLMQYVATFVVCLGAISAGTALAWTSPVLPQMTAPNVTVNASATAQNTTLDGELYLTPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU65</accession>
    <name>T1GU65_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007273-RA">
        <property type="protein sequence ID" value="MESCA007273-PA"/>
        <property type="gene ID" value="MESCA007273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530056_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9431" checksum="12E50CDC71FD429C" modified="2013-10-16" version="1">MYMMVCSISFHSFIHSLVQKDNESNFSSILRSPSGGIRRELNKKRGVILNCNELIRIVSEGGITSNIHVTIMSMNRQIDSACLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUA5</accession>
    <name>T1GUA5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUA5"/>
    <dbReference type="STRING" id="36166.T1GUA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007315-RA">
        <property type="protein sequence ID" value="MESCA007315-PA"/>
        <property type="gene ID" value="MESCA007315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_954060_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051376">
        <property type="entry name" value="CWC25_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="292" mass="34885" checksum="86352D65B8A5EFCD" modified="2013-10-16" version="1">MKKNATAKELEKILESKYQKISKELDKAAKNKKKKSKKKRKSSSDESSSNTPSPEPKRKYKTHSSRSSQTSRSKSPAKKRFSRSVSPNRKFSYHRRSRSLSRTPVDKNLDRKRSRSRSFHRKSSRSPHQLRHKNNPFKSEPRNFSRSPRKDNPFQKNHSHFGRKSISPRRRSPNSYQNTRIQSNRSNSRSKNWGNPFSSSKHSEEEKQRRLKEMTQNAAWRDEDRTRHVQKYREDYRREEDDHRKREFDNDFVNKQVHKAYDSQNSVESRLKSHKNNIQRSAHSMNSNFARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUL4</accession>
    <name>T1GUL4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007430-RA">
        <property type="protein sequence ID" value="MESCA007430-PA"/>
        <property type="gene ID" value="MESCA007430"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1095268_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052004">
        <property type="entry name" value="Dynein_assembly_factor_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46492">
        <property type="entry name" value="DYNEIN ASSEMBLY FACTOR 4, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46492:SF1">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007430-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="249" mass="29564" checksum="B6CA2D7F2F4B5253" modified="2013-10-16" version="1">MVKVTQTEDSIKIVIEINRLIKRKPDIVLFQDYVKFNNPPIFFERYLCHEIDEMKSFCRIYKTEAQLFLVKKTPGIWDEMFRTFSKEELMEKRKAVNERIIATNQQRDKEAVERFERKKRAEIEREIQRETNEREKIKSAEIESIKETIKIDGTRKDTSLVDNPKEKDNDFGLENAGNLEKNNLEEIVQMPKLIEMEPKQMRSTGKINIVFLRYFNTEIGKRTPPKWPEICTRIRLSLTTKSLACPESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV89</accession>
    <name>T1GV89_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IWS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02136759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV89"/>
    <dbReference type="STRING" id="36166.T1GV89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007678-RA">
        <property type="protein sequence ID" value="MESCA007678-PA"/>
        <property type="gene ID" value="MESCA007678"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106415_2_0_1"/>
    <dbReference type="OMA" id="VIMFMSK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000001">
        <property type="entry name" value="IWS1, SUPT6H interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="11">
        <location>
            <begin position="48"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007678-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="18113" checksum="ED2FF38387E9A878" modified="2013-10-16" version="1">MKQAAQEDRDLNMRKEPATRKISMLKSVMSQLIKKDLQLAFLEHNILNVLTDWLAPLPNKSLPCLQIRESILKLLTDFPTIDKSYLKQSGIGKAVMYLYKHPRETKFNRERAGRLISEWARPIFNLSTDFKAMSKEERQQRDLEQMPRKPRKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB2</accession>
    <name>T1GVB2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007703-RA">
        <property type="protein sequence ID" value="MESCA007703-PA"/>
        <property type="gene ID" value="MESCA007703"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190180_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054721">
        <property type="entry name" value="GEO12453p1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22861">
        <property type="entry name" value="GEO12453p1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577663" evidence="1">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007703-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8363" checksum="E5CDE0AF4B250416" modified="2013-10-16" version="1">MMKFLTICFFALVVAVSAKPQIIAPVTYSSPVAYTSPLGFASTYTYAAGFPYVASPYYAGYSTYPYVSPYFIRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW70</accession>
    <name>T1GW70_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW70"/>
    <dbReference type="STRING" id="36166.T1GW70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008042-RA">
        <property type="protein sequence ID" value="MESCA008042-PA"/>
        <property type="gene ID" value="MESCA008042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021713_1_0_1"/>
    <dbReference type="OMA" id="HENSRFD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14213" checksum="5E33BCC9C5C9F4C3" modified="2013-10-16" version="1">VHEYLRAKLCSLYENDCIFDKFECCWNGNDTSIMTGSYNNFFRIFDRNSKKDVTLEASRDIIKPKTLLKPRKVCSGGKRKKDEISVDCLDFNKKILHTAWHPLENIIAVAATNNLFIFQDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWG2</accession>
    <name>T1GWG2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWG2"/>
    <dbReference type="STRING" id="36166.T1GWG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008139-RA">
        <property type="protein sequence ID" value="MESCA008139-PA"/>
        <property type="gene ID" value="MESCA008139"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485614_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24098">
        <property type="entry name" value="OUTER SEGMENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24098:SF0">
        <property type="entry name" value="OUTER SEGMENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008139-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="19732" checksum="FA7F7FC81163A546" modified="2013-10-16" version="1">MAIVSGRWSADGAGLFTVSEDGSIKIWSRSGMLRSTVIQGSDPIIYACWSPNSTSIAFCQGGNIAIKPLAANSKLLKWKAHDGLVLCIAWSAINNIIISSGEDHRYKIWDEDGTNLFTSSPEEFSMTSLAFCPTKNLIAIGGFNNLKLCDLSGWVYDSKSFNDEKVGSLQSISWSPDGTQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWL1</accession>
    <name>T1GWL1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWL1"/>
    <dbReference type="STRING" id="36166.T1GWL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008188-RA">
        <property type="protein sequence ID" value="MESCA008188-PA"/>
        <property type="gene ID" value="MESCA008188"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_674919_0_0_1"/>
    <dbReference type="OMA" id="TCELENT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF32">
        <property type="entry name" value="NEUROMUSCULIN, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="49"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="171"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="269"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="408" mass="45324" checksum="7343378AC66714EE" modified="2013-10-16" version="1">MTLTRVDSPTYNETDGLFTAKLELSLPLTRLDLANTLECRVESSALEEPVKSHMQIDLQVIELSGVEHHTVQGSACVFTCEVFGARPAANITWTNSSNVIDPENEMDFAEITTIATEQTDGTFITKSQLQFTATRFENDAVFRCDADNVVLRDNRERPIYKSQTLEVMYPPVVRVSPKEITVNTSDAVLLNCQYIANPASLTSVKWYKSGELVDVSASHRYEGGNPENVALAIKETENGDSGNYTCELENTIGKGVSETEIELDVLYVPVVEVRMEPEGPVKESDGTNVTLYCSIIEANPSTLLKVRWYANSTLLKELPDCNETNEDLCHIDPSKLLLESIGRSFFYNYSCEGYNAAGWGPRSEDKELVVNYEPGHATLKQYPVVAVKKKSVTLTCGVEDRGYPPTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWL6</accession>
    <name>T1GWL6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008193-RA">
        <property type="protein sequence ID" value="MESCA008193-PA"/>
        <property type="gene ID" value="MESCA008193"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940507_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008193-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="15140" checksum="E3A5F3AA79BF935B" modified="2013-10-16" version="1">MLSNSQLYLVDNLQQLLQPRKKQSKSALRNSPHLPERMQPGYFLNFCTEMLFALQQSHFLWKKYAKCEMANLRLEFRTGANLFSWIPFRTLDPKGIKFKNAFIADPEVVGNAPVHPKQTVRCKFTIPARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXB1</accession>
    <name>T1GXB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02184715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXB1"/>
    <dbReference type="STRING" id="36166.T1GXB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008455-RA">
        <property type="protein sequence ID" value="MESCA008455-PA"/>
        <property type="gene ID" value="MESCA008455"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654148_0_0_1"/>
    <dbReference type="OMA" id="VVCASTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF132">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18523" checksum="0439FF501CDAA732" modified="2013-10-16" version="1">MAELFSIHEKILSLSVMTNNEFAPQIVPFMTMCFCTTLFGIFIETKTFVIWSIITISVAYLVLRLCCNAYNDMKKTAMIIHEIMQKKPPFMMASDLYYNKMKSFTLQYLHWESYFKFNGCGLFTLDYTFIFSSVSAATSYLIVLLQFDMTSILKSEGLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXC5</accession>
    <name>T1GXC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PAX-interacting protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">PAX transactivation activation domain-interacting protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02376920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXC5"/>
    <dbReference type="STRING" id="36166.T1GXC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008471-RA">
        <property type="protein sequence ID" value="MESCA008471-PA"/>
        <property type="gene ID" value="MESCA008471"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521538_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051579">
        <property type="entry name" value="DDR_Transcriptional_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196">
        <property type="entry name" value="PAX TRANSCRIPTION ACTIVATION DOMAIN INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196:SF1">
        <property type="entry name" value="PAX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="6">
        <location>
            <begin position="54"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008471-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="20141" checksum="B45203AF8ED435C9" modified="2013-10-16" version="1">AVCHVDYVLKSNWLIDSAKAGKFISPEPYTIQYIDVDDLRYDLSKVLYSQSRSTLFAGKYFFITPDVFPSSRDIEKMIKSAGGIVEPKRRSAVVIADSQARCPDTYIIITCVKDLSLIYDLIRNEKKPKCLILSTEFVMSSILTQKLEIESSKLEFYSTVNNNNNSNALNNNYNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXM3</accession>
    <name>T1GXM3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008576-RA">
        <property type="protein sequence ID" value="MESCA008576-PA"/>
        <property type="gene ID" value="MESCA008576"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2910783_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008576-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7190" checksum="698593A6E698EBA3" modified="2013-10-16" version="1">ETWGVYTEYIVLSSEIQTVNEIWEVWIVELATSRVAHGETLLYNIGSSFSNIKKHRTTNFMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXM7</accession>
    <name>T1GXM7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008580-RA">
        <property type="protein sequence ID" value="MESCA008580-PA"/>
        <property type="gene ID" value="MESCA008580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673910_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8519" checksum="4500FFDA5152E03E" modified="2013-10-16" version="1">MFPLYSLRTVPRSCVGSNFIYANLSLPRFSSFVSTAINSFFVHGSTTSPHPVPDSTHQSWNRNKPRPHHKDMLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXR9</accession>
    <name>T1GXR9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXR9"/>
    <dbReference type="STRING" id="36166.T1GXR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008624-RA">
        <property type="protein sequence ID" value="MESCA008624-PA"/>
        <property type="gene ID" value="MESCA008624"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514287_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008624-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="20817" checksum="8107E700FA06EEB3" modified="2013-10-16" version="1">MSDSILEEIKLLLPSIEDVEGILELYPPGQFIIESSLKNLENLDSEDLLESLRLKLWESINTGKFSDVSVHFRQIYSLTCFLLIYKKIFYRDSMESCFEILDFSILIGSIENLYKNASKFVDKVTEFLEEEIGQEKNIDFPIIEKLERLNFNSDIPIENCPSIESFYKQYFLMEKPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYG4</accession>
    <name>T1GYG4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008891-RA">
        <property type="protein sequence ID" value="MESCA008891-PA"/>
        <property type="gene ID" value="MESCA008891"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1791697_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008891-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16144" checksum="10293155300A1ECD" modified="2013-10-16" version="1">KIHGCQELAVCWICLKRCSLDFSEADSILGNLKSIICLHAVTNDWLIYSRSKARVTYQVTSLSAHQKYFPSLWRVTCQSVLFQALSEAYIKSVGLQANGEKKEYMVSSLRASSQERLGHKVNIGVFPHPQTGDPLCEVGRNLGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYQ9</accession>
    <name>T1GYQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYQ9"/>
    <dbReference type="STRING" id="36166.T1GYQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008996-RA">
        <property type="protein sequence ID" value="MESCA008996-PA"/>
        <property type="gene ID" value="MESCA008996"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822049_0_0_1"/>
    <dbReference type="OMA" id="RSIVAFY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17208">
        <property type="entry name" value="FERM_F1_DdMyo7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051724">
        <property type="entry name" value="Actin_motor_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049">
        <property type="entry name" value="AGAP003327-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049:SF10">
        <property type="entry name" value="MYOSIN VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="1">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008996-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="38641" checksum="2F6F5619688B8382" modified="2013-10-16" version="1">MDGQYHSVEFHPSSTAREVMELVKSKIGLPESAQGYAIYEVLGSLERSLLPEEKVADVMSRWEKYNNAAAQAAQQIQQTNQTNGNRRQHYIFLFKKHLFCDHYLNFNDPVEKELLYHQVLHGLRSERYPITEMEAVMLTALQGQLELGDCTETLQDYRTVASHCLPPRFVPNIPHEAVAMHHQSLRGTSATDAKKSFLNLIQSWPLHKATIYDVMQSFTSNWPRILWLAVDQKGIHLLEHRSRNTLCTYEYASILQYSPHLNCLMIITGTEKKQSKIILTTSQAFQIANLIREYSQVVRQNLTDHKKTVLPPPPVPEHSIGLTTSKKGSKIQFATKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYY0</accession>
    <name>T1GYY0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYY0"/>
    <dbReference type="STRING" id="36166.T1GYY0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009068-RA">
        <property type="protein sequence ID" value="MESCA009068-PA"/>
        <property type="gene ID" value="MESCA009068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796880_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine-threonine/tyrosine-protein kinase catalytic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="8226" checksum="FD55D2456D3F75FC" modified="2013-10-16" version="1">MTNAEVLSQVDRGYRMTCPPNCEPRLYEIMLECWHADPMRRPTFETLQWKLEDFYTLDQSDYKEAQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ02</accession>
    <name>T1GZ02_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015444">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009091-RA">
        <property type="protein sequence ID" value="MESCA009091-PA"/>
        <property type="gene ID" value="MESCA009091"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226211_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12038" checksum="9CC444E9C682B407" modified="2013-10-16" version="1">MKYSKLSTTSTYIEVVSPSLKELLLLINFGVAQLPDYILLLIRIFIVKLTFFRDFECNVPEILYKIPIVRLQLVSFGKKLTQPWISGSRHLSLSALSSSSITNEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZN7</accession>
    <name>T1GZN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZN7"/>
    <dbReference type="STRING" id="36166.T1GZN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009341-RA">
        <property type="protein sequence ID" value="MESCA009341-PA"/>
        <property type="gene ID" value="MESCA009341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_673179_0_0_1"/>
    <dbReference type="OMA" id="YTECKAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588620" description="Chitin-binding type-2 domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="28"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="256"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="349"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="111"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="409" mass="46135" checksum="EAF6D70CB71FB1E2" modified="2013-10-16" version="1">MGYLYILAIFCAISLTSANSFLRYLISYADCDNVPYGWLVRNPLDCGSYFVCEDEIQLEKCPSLLHFDAVKRVCNWPDKAKCDWTALPTIIIPDIDEYLPSDSWQEKETTVVHNPTTTTVKTTEIPTTTPRPTTTTSKPSTHKPTTTTSIPSTPRPTTTTPKTSTETPTTTTSKPSTQRPTTTTSIPSTSKPSTQKPTTTTVDWPKTTTESYPLSWLCENVVLHDHKVGEVESSFGAFDKEAGKAIDPMKTYDPSKVKCLYEGVYFLPHPTACDNYYICAHRTLIAHSCGTGISWNYKTNKCDFQLKSNCYSKDTGKHTTKYPSPPTEDTHTTRYVTPEKPKTTLNPNLPICPMHEQTYYPHHEDCRKYYICISGLPVLTTCPDELFWDDVHKICNLPQYTECKANKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0H8</accession>
    <name>T1H0H8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0H8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009656-RA">
        <property type="protein sequence ID" value="MESCA009656-PA"/>
        <property type="gene ID" value="MESCA009656"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901483_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15520" checksum="8EF801A2EFF10C5C" modified="2013-10-16" version="1">ENYYFKGTGENKPVSFPLNRLRNNKANQNDIFRGSFIVNPEKYGTVWISFKPVGEYAKDEEYQVVFYPELVSIGTIGASKARLDFPEKLKGWVSTEISVRSDGFVEVYIDGAQEPLRYRLEDIVRNRPNFLELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0V6</accession>
    <name>T1H0V6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RecF/RecN/SMC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0V6"/>
    <dbReference type="STRING" id="36166.T1H0V6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009800-RA">
        <property type="protein sequence ID" value="MESCA009800-PA"/>
        <property type="gene ID" value="MESCA009800"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_10_0_1"/>
    <dbReference type="OMA" id="KCNEDLK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF172">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="7">
        <location>
            <begin position="37"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="189"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009800-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="474" mass="54388" checksum="19906A651FFB0774" modified="2013-10-16" version="1">MSGGGKTQLRGKMGEKVQTKTSENANASISQRDIDSMNEKANALQNQVNYLQEEEGSLEKTLRQLKQNLQSKESELNKLQITVKSLEAQMPKLEIQMKQQKVRMEKTCADAQKVQILEEKILEKKTIFEEADSNKKIVNDQIEEINSEVNKIHGDVIKSVQTKITSTTKQITTLKNNISKLNVQSEEDIVKLAADRSKYDEEIEELSSKLEEAKKELETASNGSSEIQKEIIAIQKKESDAKLLRIEIEKKLTEVKEEFSNCKNYIPQIQRQIDSLQISPIPAGCSEEQPQIPILTDEELINIEKNKLEQKIEDNMNILGDKKANIEVINEYLQKRDIYMERVKILEDITNKRNALRNTFEDVRKKRFNEFMRGFGVITRRLKEMYRMITQGGDAELELVDSMDPFNEGVSFTVRPNKKSWKNISNLSGGEKTLASLALVFALHYYKPSPLYFMDEIDAALDFKNISIVAHYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1E0</accession>
    <name>T1H1E0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02162570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02162571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1E0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009999-RA">
        <property type="protein sequence ID" value="MESCA009999-PA"/>
        <property type="gene ID" value="MESCA009999"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1791704_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009999-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16002" checksum="0B997762E3F9BA69" modified="2013-10-16" version="1">FFTDSIFRIAGEIDAAINRYQRAIGKSPSVNTEATNTELMCGLMDDLKSNGQTSNSLNDLHDVFSKITVKEDKSNSNDVDSLLLNSDVLLPLPMMSTQKNENLVSTPIESRSKSRLSDLDKLSEDLIKEQLEAINGKKALITKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1F1</accession>
    <name>T1H1F1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1F1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010010-RA">
        <property type="protein sequence ID" value="MESCA010010-PA"/>
        <property type="gene ID" value="MESCA010010"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574630_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19989" checksum="421882A381FFB31A" modified="2013-10-16" version="1">MSTQQQEVITIDLQEIEDREEGEIVDEFEYILSSEEDEESITKRIRELEDRNDELEKIATISGAVTNYPTDVFEISDFEEEEERYYSSKGRPFHPSKSYVPETNYKHTKRSKPIKKRRSKRKKKIRVCVDESASSDSFDEENNSKVSKDVLRFAVETQNIVAPNKRNLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1H4</accession>
    <name>T1H1H4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin-related protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H1H4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010035-RA">
        <property type="protein sequence ID" value="MESCA010035-PA"/>
        <property type="gene ID" value="MESCA010035"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_3_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031252">
        <property type="term" value="C:cell leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000006">
        <property type="entry name" value="Actin-related protein 3 (ARP3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010035-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30870" checksum="BA964AB19AF82AAC" modified="2013-10-16" version="1">MTGDNNISHNXXXXXXXXXPEDHHFLLTEPPLNTPENREYTAEIMFETFNVPGLYIAVQAVLALAASWVSRAADERTFTGIVVDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRNITSFIQSLLREREVGIPPEQSLETAKAIKEKHCYICPDIAKEFAKYDTEPNKWIRTYKSINSITKQPFSVDVGYERFLGPEIFFNPEISNPDFTIPLSEIVDNVIQNCPIDVRRPLYNNIVLSGGSTMFKDFGRRLQRDIKRSVDTRLKISENLSEGRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1M2</accession>
    <name>T1H1M2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1M2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010086-RA">
        <property type="protein sequence ID" value="MESCA010086-PA"/>
        <property type="gene ID" value="MESCA010086"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798645_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010086-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16782" checksum="109FC04EC44F6D33" modified="2013-10-16" version="1">MPQRLFFRVIFLISERKIINNFKMPQRLFFRVIFLISIFSIDLSSSTEEGIYNFLFTNEPNTTEQSIVKTLYANSKISVVSESISRFKNVYKITDNVNLLSSRLQIRRSSKRDTSTMETLRTSLLSYGRSTTRNHGSHTVEAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1N6</accession>
    <name>T1H1N6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02025787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1N6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010103-RA">
        <property type="protein sequence ID" value="MESCA010103-PA"/>
        <property type="gene ID" value="MESCA010103"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237706_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577926" description="Chitin-binding type-2 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="23590" checksum="DCBEC3E0E7CA469B" modified="2013-10-16" version="1">MMNRIATIGIFLLLCGVTSSQYVNCYGKNAICTGPLSYKPCIDDGFGVMIVDNSRKLQLCPPGSRCRNNGSSICQRLRTIPPPTTSTTTTTTAASTSTEGVNKPTEPSIISTEFVLSSTVPTVDDLSTISTSSTTSYSTISENDIPTNSTVSISENIPSSSTTEFIFKTSESSTISSTKNLESTTTNSSTVGYGIESVDASTDMNSTVSISESTPSESFSTTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2F6</accession>
    <name>T1H2F6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="23171" checksum="632F6C2EB7FB854A" modified="2013-10-16" version="1">MNATPIGIHRQKLLFLQFLKVSLSNIGAEIGVDKTPKDGSIFQAEDEHYPIYICRVAIEGVARSGFTEKRGQKHMCMVAYLNTVKPYIQFDILVNKGKLGKVSWKKWKRYEDTPDGAVSVNDYTFVARHKVKDGDKKGGSQYDMGRLEVNSNNNGKIYVTDADSKEHHYLEGEVLVEVEPLRYELSDIELDKSKTDIKNNVTEL</sequence>
</entry>
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    <accession>T1H2V0</accession>
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        <submittedName>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
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    </evidence>
    <sequence length="51" mass="6042" checksum="8146A904BF08B07B" modified="2013-10-16" version="1">MRQIFGINVELDITKKCTSYITMQINLTKSGQDFCAGWRIIMLRRRYLMPS</sequence>
</entry>
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        <name type="scientific">Megaselia scalaris</name>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="8026" checksum="D2717109F75150D5" modified="2013-10-16" version="1">MDMQSGVFDCYEFDLQIFGFVLSTVTRLQFVPAVLFLSRLSYICIWDTPYNISCDLRKDGFLDNLSIFP</sequence>
</entry>
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    <accession>T1H571</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Megaselia scalaris</name>
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        <lineage>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <property type="gene ID" value="MESCA011446"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011446-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6850" checksum="9CB5C2841123C6E4" modified="2013-10-16" version="1">MDGNSSFEHIGGKLQFFKDGKFILELTRSKEGESFNWVSVPKKIKSATTIFAANSKHDGSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1H582</accession>
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    <protein>
        <recommendedName>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
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        <lineage>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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                <person name="Hughes D."/>
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            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with SYS1.</text>
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        <location>
            <begin position="24"/>
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        <ligand>
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        <ligand>
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        <ligand>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="162" mass="18497" checksum="82C859019A203ADA" modified="2013-10-16" version="1">MFTLLHGFYKYITQKEDYYVLLLGLDNAGKTTFLEATKAKFTPDYKGMQASKITTTVGLNIGQIDVDGIRLNFWDLGGQQELQSLWDKYYSECHGIIYVIDSEARDRMEESKVVFDKMIKNELLTGVPLLLLANKQDLPDVMGVREIKPVFQHAGILIGRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5B8</accession>
    <name>T1H5B8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5B8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011495-RA">
        <property type="protein sequence ID" value="MESCA011495-PA"/>
        <property type="gene ID" value="MESCA011495"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253083_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011495-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12116" checksum="5B84A9F96ACFEABB" modified="2013-10-16" version="1">MDTDMRICTWNIQSIYTRGAVQDLRRDLSEYKADISVLREIRWTGKGKLEDKSKFQCDIYFSCYLSKHELGVGQYSLHDDPNDNGMILNTTTVARDMVIIHKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5K3</accession>
    <name>T1H5K3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Holliday junction regulator protein family C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388599">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5K3"/>
    <dbReference type="STRING" id="36166.T1H5K3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011583-RA">
        <property type="protein sequence ID" value="MESCA011583-PA"/>
        <property type="gene ID" value="MESCA011583"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402151_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022102">
        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Holliday junction regulator protein family C-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011583-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10606" checksum="5FCC52C18D68DD0A" modified="2013-10-16" version="1">MSPDSPEPDSDYNLTPRTEAKYNKIDEDFQLMIARQIPNVQRGMPGQNYTLPVSVPVGGIMQTVRSYRLVPKWLMQLVLDLPARKQCILQAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5V0</accession>
    <name>T1H5V0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5V0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012083-RA">
        <property type="protein sequence ID" value="MESCA012083-PA"/>
        <property type="gene ID" value="MESCA012083"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050131_0_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF5">
        <property type="entry name" value="AT19154P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="3">
        <location>
            <begin position="80"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24683" checksum="C0350E86081ECD09" modified="2013-10-16" version="1">GKGPISWKSLTIGIGGAGFLGFMMYLKKEKEEALMRERKRQLGKAAIGGEWELVDSLGNVRKSKDFLGQWLLIYFGFTHCPDICPDELEKMSAVVEELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDHTIIMYLINPDGEFVDYYGQNKDKDQCVGSILINMTKWKQLQTKSWFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H627</accession>
    <name>T1H627_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H627"/>
    <dbReference type="STRING" id="36166.T1H627"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012160-RA">
        <property type="protein sequence ID" value="MESCA012160-PA"/>
        <property type="gene ID" value="MESCA012160"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_0_2_1"/>
    <dbReference type="OMA" id="EWSERCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012160-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11864" checksum="2050E9348ABABFA2" modified="2013-10-16" version="1">YEEVKDLPNHPEKLLIDVREPDELKQFGQIPTSINIPLGEVEKALNFQKKFKAQYGHSKPKPEDYVIFHCRLGRRSQTASEMLPNDSNVHNYAGSWLEWAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6M5</accession>
    <name>T1H6M5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6M5"/>
    <dbReference type="STRING" id="36166.T1H6M5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012358-RA">
        <property type="protein sequence ID" value="MESCA012358-PA"/>
        <property type="gene ID" value="MESCA012358"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754302_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012358-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="15760" checksum="652D070D42934991" modified="2013-10-16" version="1">AAQQNPYAAAAAQQGAPYVIPGQEAAPYIGLMTQMPQYYSHVTPWPVYPGNLIPQQGTQPRRPLTPSQQGAENQSYQVIPAFFDQSGSLVMGPRTGTPMRLVSPAPVLVPPGAARAGPPQGPPQLYPPQPQTAQQNLYSTQNGSNVGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H788</accession>
    <name>T1H788_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-ubiquinone oxidoreductase chain 2</fullName>
            <ecNumber evidence="4">7.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">NADH dehydrogenase subunit 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="T1H788"/>
    <dbReference type="STRING" id="36166.T1H788"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012626-RA">
        <property type="protein sequence ID" value="MESCA012626-PA"/>
        <property type="gene ID" value="MESCA012626"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521548_0_0_1"/>
    <dbReference type="OMA" id="TIMMMST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050175">
        <property type="entry name" value="Complex_I_Subunit_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46552">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46552:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="20">
        <location>
            <begin position="2"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012626-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="20258" checksum="9E293E695B0CD9D9" modified="2013-10-16" version="1">ISSLILKRGIAPFHFFPNVIEGLNINSFILLTQKIAPLILISYLITNYLFIPIILSIIVGSLGGLNQTSLRKIIAYSSINHLGIGAAIITEIRLNYFLFYSFLSITLIYFFNINKLFFINQIYSQLLKKLILFLNFLSLGGLPPFLGFFPKIVIQIISVINYFNYYIVILTLITLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7A3</accession>
    <name>T1H7A3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EMC2 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7A3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012641-RA">
        <property type="protein sequence ID" value="MESCA012641-PA"/>
        <property type="gene ID" value="MESCA012641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1647690_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17561" checksum="7A6BAD1ABDB960DF" modified="2013-10-16" version="1">EFPGSIRVKLLKSMRLEAMEMYDDALELLDWIIENDVTNAGPRKRKIAXXXXXXXXXXXXXXXXXXXXXXGKAVFCMEEVLLHNPHSHLIHQRIGEIRYTMGYYSQAVKLNPKNLRALYGLFLSYNHLANSKLAGNKRKEAQKLKQATELTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA08</accession>
    <name>T1GA08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA08"/>
    <dbReference type="STRING" id="36166.T1GA08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000051-RA">
        <property type="protein sequence ID" value="MESCA000051-PA"/>
        <property type="gene ID" value="MESCA000051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2339505_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577197" description="SCP domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="10928" checksum="C894128934E8A609" modified="2013-10-16" version="1">MLVAILILFFALYSVNFNSKVAGKEYSFCDPDLCGGRRNTHIACNNYREFARSCPADANILDVSAFKESFVQAHNERRNFVALGLLPGFEPATKMATM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAD3</accession>
    <name>T1GAD3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAD3"/>
    <dbReference type="STRING" id="36166.T1GAD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000188-RA">
        <property type="protein sequence ID" value="MESCA000188-PA"/>
        <property type="gene ID" value="MESCA000188"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1483641_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="29"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="21086" checksum="2476F3FE3E7416E2" modified="2013-10-16" version="1">MEPGLSKVKARSIKPVPSSNLKVNSKFKILSHREYVKWANELSKRKHYKIEELSSGVAYCIILNNIAPKAMPLKHVKMDPTSKPESIHNLRLLQAALMKLKCEKEIPISNIVEAHYKDNFAFAKWFKQFYDSLIEHKNIDIEPAKIKIEPKAIKIEPTENIDFNLKYEKFIISKGIKNEKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAU0</accession>
    <name>T1GAU0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02125219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000352-RA">
        <property type="protein sequence ID" value="MESCA000352-PA"/>
        <property type="gene ID" value="MESCA000352"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798649_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16145" checksum="97085A59C947E1F9" modified="2013-10-16" version="1">MVSLGPVTQPYSLRKIFGGIKLSGLVSNLFINTYFYFVLMKLRQIQADNTLPILLSFWLIVCSYVDLFVLCLSSLDLTMAFFGGLVYAFLSVGRSMLFENEQIHPSSPHVPPRPSKALCANPLGYSRDVAVVVHQYSSTIRYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC08</accession>
    <name>T1GC08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02029468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC08"/>
    <dbReference type="STRING" id="36166.T1GC08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000803-RA">
        <property type="protein sequence ID" value="MESCA000803-PA"/>
        <property type="gene ID" value="MESCA000803"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040429_0_0_1"/>
    <dbReference type="OMA" id="GIEMRNM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052250">
        <property type="entry name" value="PDI_TMX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426:SF1">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00312">
        <property type="entry name" value="CALSEQUESTRN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000803-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="386" mass="45078" checksum="F8F89C2DDF106AB7" modified="2013-10-16" version="1">MFYAPWCGYCKKTEPIFNLVSQALHSTNIRVGRLDCTKYPSVPKSLPIRGYPTIMFFKGNQEFVYNGDRTKEDLVDYSLRMSSPAIQLVTRVESIDMLKTSHKSFFLFVGEQIGTVWTTFFDVAEFYQEHGFFYATSEEIASQHFDIEHVPALLVHKEETHYFYPHGHKAHEMTTKEVNETLTQWVNNERFLTFPKITRFNIRELMSTKKFLVIAVVEEDKLSQVATHELEFRDMIEEVIRKYRHRFVPRQVPIRMDRRSWYCPFQVMDTLPTPHLIVINSTTQHHHIPEDDPMQMTINSIHVFLESIHNQSAVAYGGDSFAVRAYRAYFETKKGLVELWKGNPVLTSVLFGLPLGFLSLILYSIFCADCMDAPEEDDEDDHEKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC95</accession>
    <name>T1GC95_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02091660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC95"/>
    <dbReference type="STRING" id="36166.T1GC95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000903-RA">
        <property type="protein sequence ID" value="MESCA000903-PA"/>
        <property type="gene ID" value="MESCA000903"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128552_0_0_1"/>
    <dbReference type="OMA" id="MHTITRM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007185">
        <property type="term" value="P:cell surface receptor protein tyrosine phosphatase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09498">
        <property type="entry name" value="SAM_caskin1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000071">
        <property type="entry name" value="Caskin, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000028">
        <property type="entry name" value="caskin-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035498">
        <property type="entry name" value="Caskin1/2_SAM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24155:SF11">
        <property type="entry name" value="CASKIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24155">
        <property type="entry name" value="OSTEOCLAST-STIMULATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="1">
        <location>
            <begin position="14"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="1">
        <location>
            <begin position="83"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000903-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="180" mass="20746" checksum="AFCBBDA91055FFD5" modified="2013-10-16" version="1">PRCSSVSSCSIGSVDRNDILDWLIEVKFEDYSQLFISAGYDLPTIARMTPEDLTAIGITNPQHRERIKKHIDKLQIVDNLPDFVPGSIEEWLRLLRLEEYIQPLLEQNYKTVRDVTQVTWEDLEDIGIVKLGHQKKILLAIKRVKDILSGKRNHQIYSTVGLLPPIQHSICFLQQKTNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCA9</accession>
    <name>T1GCA9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glucuronic acid decarboxylase 1</fullName>
            <ecNumber evidence="5">4.1.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">UDP-glucuronate decarboxylase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-xylose + CO2</text>
            <dbReference type="Rhea" id="RHEA:23916"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="EC" id="4.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:23917"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02186415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCA9"/>
    <dbReference type="STRING" id="36166.T1GCA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000919-RA">
        <property type="protein sequence ID" value="MESCA000919-PA"/>
        <property type="gene ID" value="MESCA000919"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1043122_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00796">
        <property type="reaction ID" value="UER00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033320">
        <property type="term" value="P:UDP-D-xylose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000065">
        <property type="entry name" value="UDP-glucuronic acid decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588182" description="UDP-glucuronic acid decarboxylase 1" evidence="19">
        <location>
            <begin position="23"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="20">
        <location>
            <begin position="109"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000919-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="267" mass="30657" checksum="1073599B6FE47702" modified="2013-10-16" version="1">MKVIGGVVAVFLLILIVYKLNSDESKISDILRIKENYYEDETTDKKVPEKKISERDSLYQHKLELEITQRKLIALEEKIRALEESPSKGLGKRYPPVKYLNYKNRKRILITGGAGFVGSHLVDVLMQQGHEVVVADNFFTGRKRNVEQWLGHPNFELIHHDIVNPLFIEVDEIYHLASPASPPHYMYNPVKTIKTNTMGTINMLGLAKRVMAKVLVASTSEVYGDPTVHPQPETYWGHVNPIGPRACYDKENVCRKLYLMLMLNRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCH2</accession>
    <name>T1GCH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000986-RA">
        <property type="protein sequence ID" value="MESCA000986-PA"/>
        <property type="gene ID" value="MESCA000986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963440_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6431" checksum="C63F421620CDFF4F" modified="2013-10-16" version="1">MHPQSDPLIVFTHCCLAIMGSCSFPTFEMKSSITNFISKTGMVEYTSSSSSEKMSLTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCN3</accession>
    <name>T1GCN3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4766 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCN3"/>
    <dbReference type="STRING" id="36166.T1GCN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001053-RA">
        <property type="protein sequence ID" value="MESCA001053-PA"/>
        <property type="gene ID" value="MESCA001053"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1191063_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031903">
        <property type="entry name" value="DUF4766"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15973">
        <property type="entry name" value="DUF4766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576823" description="DUF4766 domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4766" evidence="3">
        <location>
            <begin position="48"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001053-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="233" mass="23450" checksum="738BB3D3C90D19B1" modified="2013-10-16" version="1">MKVFICLLSCAALASAGFLGLGGGSGGGSSGGHGGWSSGGGHGGHSGGGGGHGGEVKIIKVIQEQGHGGHGGGYGGGHGGYGGGHGGGHGGHQEVKVIKVVHEQGDKVLMLGSLGFCCRKASLSSACLAASVGKLGLTFLMQPAGIAAFNMFFRKVRSALCASVGMGTSAKVSSNHSQKAGQLALVKLKYDLCLLFNDKSLKLHAVWIYAPKPPITSSDGQRTQLQFYISIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCV0</accession>
    <name>T1GCV0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001123-RA">
        <property type="protein sequence ID" value="MESCA001123-PA"/>
        <property type="gene ID" value="MESCA001123"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3056458_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001123-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="54" mass="5933" checksum="DA63B402CB7EF2F5" modified="2013-10-16" version="1">MWPIFVGVQILKTLLLAMFLPSLIGSLGKIVGKGLTQVSGFNRPSETIENLEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD32</accession>
    <name>T1GD32_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Queuosine 5'-phosphate N-glycosylase/hydrolase</fullName>
            <ecNumber evidence="6">3.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 6">Queuosine-nucleotide N-glycosylase/hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the hydrolysis of queuosine 5'-phosphate, releasing the nucleobase queuine (q). Is required for salvage of queuine from exogenous queuosine (Q) that is imported and then converted to queuosine 5'-phosphate intracellularly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>queuosine 5'-phosphate + H2O = queuine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:75387"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:194371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5 6">
            <dbReference type="Rhea" id="RHEA:75388"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the QNG1 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02060306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02060307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD32"/>
    <dbReference type="STRING" id="36166.T1GD32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001211-RA">
        <property type="protein sequence ID" value="MESCA001211-PA"/>
        <property type="gene ID" value="MESCA001211"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264371_0_0_1"/>
    <dbReference type="OMA" id="RIRACTY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019438">
        <property type="entry name" value="Q_salvage"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314:SF0">
        <property type="entry name" value="QUEUOSINE 5'-PHOSPHATE N-GLYCOSYLASE_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314">
        <property type="entry name" value="QUEUOSINE 5'-PHOSPHATE N-GLYCOSYLASE_HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10343">
        <property type="entry name" value="Q_salvage"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00035119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001211-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="25218" checksum="D0218E82BF380FDC" modified="2013-10-16" version="1">NDTKWKVDGQTGYFGLCAAITRALKKGVDVTNSKVYSKFTEEELLEILKSDDGETVCPLIKERVECLRQVGSKLLEKYDGRFENCVKICNNESVKLLDLIVTEFPCFADEATFKDKRVSLYKRAQILIGDLWACFEGQGIGYFKDIDQITMFPDYRVPQVLVHFKSLEVVCWPLREYTERIRACTYGPHIKDLCSMSDITQHDVRKGLGREQPRIQDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD39</accession>
    <name>T1GD39_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001218-RA">
        <property type="protein sequence ID" value="MESCA001218-PA"/>
        <property type="gene ID" value="MESCA001218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940514_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="15355" checksum="10E392861A404B93" modified="2013-10-16" version="1">MEGRHRLYTPKEYMESIAAYKNTANNANEAHQRDRNLSWCGILNRKIIGPYFKEENLNGKWIGYIRNDLNLFDCCPRSPDLYPLYFFLNLVYKTELNILESIEEGKVLHFVVINVKLEQSLRVGRNNDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD56</accession>
    <name>T1GD56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02046445">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD56"/>
    <dbReference type="STRING" id="36166.T1GD56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001237-RA">
        <property type="protein sequence ID" value="MESCA001237-PA"/>
        <property type="gene ID" value="MESCA001237"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1361812_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000359">
        <property type="entry name" value="GLIS family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000425">
        <property type="entry name" value="PLAG1 like zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001237-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="23045" checksum="4A10A23C78B265FE" modified="2013-10-16" version="1">MCQLCQKCFSRAENLKIHMRSHSGEKPYVCPYEGCNKAYSNSSDRFKHTRTHSTEKPYVCKVPGCQKRYTDPSSLRKHVKTFKHTPVTEDTNTKDEEPQIKSESSSTPSPPIQIPIPVMPAVVYPSAGYFYHQTASQDFNYRKYDSFYYGTEPQMAENLSTSTSPHFWMEGASIKRDSHFIVPSLPSPMELDAPLDLSFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE59</accession>
    <name>T1GE59_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA topoisomerase</fullName>
            <ecNumber evidence="3 8">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 8"/>
    <dbReference type="EMBL" id="CAQQ02001837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE59"/>
    <dbReference type="STRING" id="36166.T1GE59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001617-RA">
        <property type="protein sequence ID" value="MESCA001617-PA"/>
        <property type="gene ID" value="MESCA001617"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_1_3_1"/>
    <dbReference type="OMA" id="ACSTHEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000002">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 3-BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="10">
        <location>
            <begin position="3"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="11">
        <location>
            <begin position="169"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001617-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="520" mass="58929" checksum="B269801AF2E4740A" modified="2013-10-16" version="1">MKVVLMVAEKPSLAASIANILGSGKCSQRKGSNNACSVHEWKGTFQGESVLFKMTSVCGHVMTCNFTGKYNSWDQIDPVELFSCPIQKQEANPRLHMNSFLEREAKGCSYLVLWLDCDKEGENICFEVMNAVSRTIPNIYSKKVTFRAHFSAISDKDIMKAMASLGHPDENQSKSVDARQELDLRIGCAFTRFQTRFFQGKYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPENYWCLSLQTSNPSAHFEWKRKRIFKRDIAMLLLNQIKKIKEAKISKCVTKTEFRKRPQALNTVELMKAASVGFNLSPHATMQIAEKLYIQGYISYPRTETTAYPQNYDFSSTVRQLKEIPDYSAEADNILKNINSPRSGKDCGDHPPITPMKAGQRSSFDNDSWKIYDYVCRHFLGTISKDLKYSEVTTEIVVETETFVNTSKCLIDPGYTSAMNWMAFKNDESGAKFTEGQTVEINDIKISEHQTSPPDYLTESDLISLMEKHGIGTELLFPCISTIFPREIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE86</accession>
    <name>T1GE86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02106714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE86"/>
    <dbReference type="STRING" id="36166.T1GE86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001644-RA">
        <property type="protein sequence ID" value="MESCA001644-PA"/>
        <property type="gene ID" value="MESCA001644"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834061_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001644-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7813" checksum="7AC8A404FFE2348C" modified="2013-10-16" version="1">MHRIPYSPSNGKSYKAKGVVYMMHDAGYDVWLRNFPGNTYSKRDVILTPSMSAFWNFDLYEFGYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GED1</accession>
    <name>T1GED1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CLIP domain-containing serine protease</fullName>
            <ecNumber evidence="10">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02101079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GED1"/>
    <dbReference type="STRING" id="36166.T1GED1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001692-RA">
        <property type="protein sequence ID" value="MESCA001692-PA"/>
        <property type="gene ID" value="MESCA001692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_0_3_1"/>
    <dbReference type="OMA" id="IANIGYE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022700">
        <property type="entry name" value="CLIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038565">
        <property type="entry name" value="CLIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12032">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00680">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51888">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Clip" evidence="13">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="94"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="346" mass="38909" checksum="4C36854D59650953" modified="2013-10-16" version="1">NIFKTCTNSRNRNGSCIYLSECYELKILAETAPVSAEDTLFLRNNQCGWEKDKVLICCTNKQRNKKFNKSPNHNLPKLGDCGKGLTQNKFGIKIINGHETLLEEFPWMALIEYELHYSRSFRCGGSLISDRYVLTAAHCLQIPKGQSNWKPINVRLGEWDIAKDDDCYNGRYSDPPLNVLLNIQFYNEDTISNDIGLIRLASKVQFSAFIQPICLPFGFDSKMRNTDLYVAGWGMTEKGRNSNIKLKGLVHVVNSKTCADKFAEANMEISSTQFCVIGGDTTKKIDSCRGDSGGPLVLWNKTSSTGSEYFLLGIVSFGNSRCDSLEWPSVNTKVSRFLNWIVNNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEI9</accession>
    <name>T1GEI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001753-RA">
        <property type="protein sequence ID" value="MESCA001753-PA"/>
        <property type="gene ID" value="MESCA001753"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742965_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001753-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7957" checksum="CE767F87C7F30550" modified="2013-10-16" version="1">MPVPDQEEVPPYTSIESNQQLIPMQTYRSENQRDSASGPVAGVVLRRNEANNDSGDENDGENLNEEMNNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEY6</accession>
    <name>T1GEY6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001912-RA">
        <property type="protein sequence ID" value="MESCA001912-PA"/>
        <property type="gene ID" value="MESCA001912"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760702_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001912-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8170" checksum="8CD223B8910D69CB" modified="2013-10-16" version="1">MHTTFIASYPEFATNRTAKNFFLDAGKTTIPTIINTIIFLFDLHSIFRAILRWSNVVENYLKIICINFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFU7</accession>
    <name>T1GFU7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02115946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFU7"/>
    <dbReference type="STRING" id="36166.T1GFU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002247-RA">
        <property type="protein sequence ID" value="MESCA002247-PA"/>
        <property type="gene ID" value="MESCA002247"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_11_6_1"/>
    <dbReference type="OMA" id="GKPLNCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:ATP synthesis coupled electron transport"/>
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        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
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        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
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        <property type="entry name" value="Mopterin_OxRdtase"/>
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        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
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        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
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        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
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        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
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        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
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        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
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        <property type="entry name" value="NuoG"/>
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        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
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        <property type="entry name" value="Fer2_4"/>
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        <property type="entry name" value="Fer4_NDSU1"/>
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    </dbReference>
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        <property type="entry name" value="Molybdopterin"/>
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    </dbReference>
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        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
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    </dbReference>
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        <property type="entry name" value="4Fe-4S ferredoxins"/>
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    </dbReference>
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        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
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    </dbReference>
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        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_HC3"/>
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    </dbReference>
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        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="6" id="KW-1278">Translocase</keyword>
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        <location>
            <begin position="42"/>
            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="160"/>
        </location>
    </feature>
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        <location>
            <begin position="262"/>
            <end position="318"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00013888"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
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        <source>
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            <dbReference type="PROSITE" id="PS51085"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002247-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="665" mass="71850" checksum="571578DAA7414A27" modified="2013-10-16" version="1">MIRAPLTKALSLGANTSPIHKIAAAAFRTTAVAAQTPAKAPEKIEVFVDDIPVHVEPGTTILQVSAAVAGVEIPRFCYHERLAVAGNCRMCLVEIEKSPKLAAACAMPVMKGWRIKTNSEKTRQAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINHTGKRAVEDKDIGPLVKTIMTRCIHCTRCIRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLSELSGNVIDLCPVGALTNKPFSFSARPWETRKIHSVDVLDAVGSNIIVSTRTNEVLRILPRENEEINEEWLADKSRFACDGLKRQRLVAPMVRVNGELTPVEWEGALIEVGKKVRAAKGAVAGVAGALADVESMVALKDLLNRVGSEAKYQSLETADVVLLVGTNPRYEAPLINTRLRKAYVHNELELASIGPKIDLSYKHNNPAIIVGSDLLARPDGAGILANLQNFAKEFKSTNVLNVLQRNASQAGALDIGYQAGVEEAIKSQPKVLILLNADEAKVTRDQLPKDCYIVYVGHHGDAGAQIADAVLPGCAYTEKTATYVNTEGRAQQTMAAVTAPGLAREDWKIFRAVSEIVGTPLPYDTLDEVRDRLDDIAPHLIRHGHLESNSFQSVAPQLSAAPSINSGKISASPTMAKCIQAAKKEGEKKAASAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFX8</accession>
    <name>T1GFX8_MEGSC</name>
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        <recommendedName>
            <fullName evidence="1">Origin recognition complex subunit 5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFX8"/>
    <dbReference type="STRING" id="36166.T1GFX8"/>
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        <property type="protein sequence ID" value="MESCA002280-PA"/>
        <property type="gene ID" value="MESCA002280"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
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        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="1">
        <location>
            <begin position="50"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="21353" checksum="9C130A83FC9042C9" modified="2013-10-16" version="1">MDVKEVSKLWRNIAAPLKSALTQVYMRMESSDENKEHQDQIKKFALNLELPYYAKYLLISAFLASHNSAKSDKRLFVKDHGKQRKRLQTVNANAKTVEKMSTSLGPKSFSIDRLLAIFYAILEDKVGISCNLLAQISTLVELKFLSFVAGENNVMDGSAKLQCTVGLDFIVYIGKVVGFNVRQYLSDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG59</accession>
    <name>T1GG59_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002368-RA">
        <property type="protein sequence ID" value="MESCA002368-PA"/>
        <property type="gene ID" value="MESCA002368"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984101_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002368-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14936" checksum="9AEC245DD031B475" modified="2013-10-16" version="1">MHDSNNKKYKFEKKRNSTYQSMLFVLEKQFNIFERDSNQINSTFLFVSRCAGCGFWQANRSLIGFLDQFFYSIGSFDATSNDKLGVLLLGINTSDNLPVITDKKQYEKFYRFYRNFPSRFFSFEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG75</accession>
    <name>T1GG75_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002385-RA">
        <property type="protein sequence ID" value="MESCA002385-PA"/>
        <property type="gene ID" value="MESCA002385"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2596955_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588274" evidence="1">
        <location>
            <begin position="25"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002385-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9001" checksum="1B915138BC90C963" modified="2013-10-16" version="1">MGLLRKRHLFAAIVILAITNGSHAQFWKETPRDSGAIQLQSEHIAPTNQYINQYTNNFELIDANYVRRGQNCSTGTVCPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGN7</accession>
    <name>T1GGN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGN7"/>
    <dbReference type="STRING" id="36166.T1GGN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002560-RA">
        <property type="protein sequence ID" value="MESCA002560-PA"/>
        <property type="gene ID" value="MESCA002560"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2187182_0_0_1"/>
    <dbReference type="OMA" id="GETMMIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF61">
        <property type="entry name" value="SD03967P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="8"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002560-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14162" checksum="13435172081B196A" modified="2013-10-16" version="1">MSEIATGVTGVVRVNNQEISTNSESFRKLSCYIHQDDLVRPMLSVGETMMITAHLKLGYKVSQEYKLNIVKKILVLLGLDHRYHTYTVKLSGGQKKRLAIALELICNPPVLYLDEPTRLSGIGQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGU3</accession>
    <name>T1GGU3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Metalloendopeptidase</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02142143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGU3"/>
    <dbReference type="STRING" id="36166.T1GGU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002624-RA">
        <property type="protein sequence ID" value="MESCA002624-PA"/>
        <property type="gene ID" value="MESCA002624"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017286_2_1_1"/>
    <dbReference type="OMA" id="YRCENGS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="8">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002624-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="25186" checksum="A79E9F0E2935D192" modified="2013-10-16" version="1">MTWPKATIYYKFHDKFNSQYKTKIMKALKKIMEVSCLKFKEGGTWFGLGPHIKIKRDDKQCSSVVGYKGYFGEMNMYLGKCDEIGIIHEFLHAAGLKHEHNARIRDKYININWSNIIPKKQSNFQKYNIDTNFGLPYDFESIMHYASNSLSKNGKPTIVRKNNKKRYITKNKKMSKTILYNAENAKKIIDNCPWDNPSIVWEEIIIIILTLEGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGU5</accession>
    <name>T1GGU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGU5"/>
    <dbReference type="STRING" id="36166.T1GGU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002626-RA">
        <property type="protein sequence ID" value="MESCA002626-PA"/>
        <property type="gene ID" value="MESCA002626"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_513505_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="127"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="227"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="340"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="387"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002626-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="531" mass="61119" checksum="8B51F716846587FE" modified="2013-10-16" version="1">ANEIYVKSTKCWKVAFLLNFAIPSLSQSFRNIPQDMNGAVDNGQLLAAKQPKNFKLIVDPALIKGAQMKIHRFDGVVPNDPTYPPVIPRDPRNPLVRVRPKLLDAIELLIPRFRIDSNYTGQPPPIEITITNLNDNIDKQFLSGMLEKCGPYDEMLIHYHPENKKHLGIARIVFENVKAARACIDRYNEKSSTYEYDRLYSDSEEEREYQEKRRRNTEYMEQIEREFQEEQERNRKEGGNHEIPPTPGVDLLSAANVFAPVPPSSPPKKEVKVVEEKKEKKEKPEKKKDLPPPPKKERKKPEKKVKDQIHADLIKMSPSASSDGGSSQASYITFEHDYCLPPTSYVEDNNPSLLAHDHGYSSAKDKPQEDDKQTAVLGPAKHGPGRPRKDSAKVKPEPKEKKPKERKRKGKDLVVDSPKENFDYKRPKFVPAPVEQNFVPREVFQARSTNEESAILFEILSKGIDQEDIDYLRRSYEKHLQDDVHGNWLNCTHWVNEVFHYIDVNGTHGDWIYYVVLWGGENIFCFVPVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHE9</accession>
    <name>T1GHE9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147374">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147375">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002845-RA">
        <property type="protein sequence ID" value="MESCA002845-PA"/>
        <property type="gene ID" value="MESCA002845"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362119_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002845-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11076" checksum="D257B4D6AC4D109F" modified="2013-10-16" version="1">MISKTSRIIGPIFTIEDYDFVAGKNFMYLGSEVISVKGGYSFANRHFPRIYEYLHKNQQLETVNNILLLAGFCRGIITSGYICYENKNQLDHLVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHS0</accession>
    <name>T1GHS0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHS0"/>
    <dbReference type="STRING" id="36166.T1GHS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002978-RA">
        <property type="protein sequence ID" value="MESCA002978-PA"/>
        <property type="gene ID" value="MESCA002978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1039332_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="3">
        <location>
            <begin position="6"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="155"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31859" checksum="EC2182C02449CEB9" modified="2013-10-16" version="1">MRCSVEFVNNPYRSYYAGNTLYGCLNIALDREKYIKVSSLRITNPCKRKGQSSFPRGRYTVKAEQTFIDEKLLVVSNTRLPAGEHTFQFESNLPADCPPSFKGKHGQIKYKAVILVPKMLCDKKIPFEFTILSYSPLPFFEANIPISIHIGPTKTVTFPKNVFRVGEHILIDINIDETKEKKDRSIKFFLVQYIWYQTKENCHSCRSQRKQKGNVIEFPVITSDYNYKQSIRIPDNLPTTLEYIEVIKIRYLLKILINKKKFKFPIVLGDYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ62</accession>
    <name>T1GJ62_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02083342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ62"/>
    <dbReference type="STRING" id="36166.T1GJ62"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003505-RA">
        <property type="protein sequence ID" value="MESCA003505-PA"/>
        <property type="gene ID" value="MESCA003505"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264373_0_0_1"/>
    <dbReference type="OMA" id="LCNCTNF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003505-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24852" checksum="FC7C463B7CBE50D7" modified="2013-10-16" version="1">MIVVIHSPEEIPDFENAILTSDNKDLSVSIIPRVIQISDGLKNVDISKRQCYLQNEKYLQHFKIYTQSNCEHECLTNLTLKICGCVKLYMPRTETTEVCGSKAHFCTENALDLLLRKALEYKGLKCDCLPSCSSIFYNIETPEREQVGNSSHKILKFYFKENNFVSWKRYEGFGANDFLASVGGVLGLFLGISVMSVIEIVYFFTIRLYTHYFGGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ87</accession>
    <name>T1GJ87_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ87"/>
    <dbReference type="STRING" id="36166.T1GJ87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003531-RA">
        <property type="protein sequence ID" value="MESCA003531-PA"/>
        <property type="gene ID" value="MESCA003531"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418432_0_0_1"/>
    <dbReference type="OMA" id="RANCASP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF3">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="87"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003531-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="21350" checksum="4501B13971008AA6" modified="2013-10-16" version="1">PDTKSNGNKVDSRRKRSKRSRSSSSSSSSSSSGSDSDEDKRKKVRSNGKNKSMSKSPSKKVGNNESGTSSGKTYDYMTKLNYLFRDTRFFLIKSNNADNVEISKKDGVWATLPQNEANLNQAFRESRNVLLIFSVNESGKFAGFARMSAESNKSLEQPKWVLPPTISAKSLGGVITIDWICHKELPFINTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ98</accession>
    <name>T1GJ98_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ98"/>
    <dbReference type="STRING" id="36166.T1GJ98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003542-RA">
        <property type="protein sequence ID" value="MESCA003542-PA"/>
        <property type="gene ID" value="MESCA003542"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1742639_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003542-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17109" checksum="6777B39FFDBD3C27" modified="2013-10-16" version="1">MTSMTVEIEPFIEPIEQSDSMDGEYSEASDGAEVDENSSQGSRRLLADGDGNLPGNNGRRWSRQEDQQLKQLIMQHGENYELIALNFKDRSEAQCQQRWTKVVNPELVKGPWTAEEDNKVIELVKRYGPKKWTLIASKENILGFQKGELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJS4</accession>
    <name>T1GJS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003728-RA">
        <property type="protein sequence ID" value="MESCA003728-PA"/>
        <property type="gene ID" value="MESCA003728"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375178_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10757" checksum="E5BA1E5AEFC80743" modified="2013-10-16" version="1">MKNCGKTEILRLQIHIICCRAVMSLDMSFCHTPGLFKVQEQIRILNSLIVFLVNSLCGFCSPETLNLVAYVVEQTDSSVVFTVRNQSAQHRIQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK19</accession>
    <name>T1GK19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable transcription factor with a role in the retinal determination (RD) network. Regulates ato expression and is required for normal R8 induction and differentiation. Danr appears to repress Dan expression, but Dan is required for Danr expression anterior to the morphogenetic furrow (MF). Dan and Danr lie downstream of so and require dac function for highest levels of expression. Contributes to differentiation of antenna-specific characteristics; effector gene that acts downstream of homothorax (hth), Distal-less (Dll), cut (ct) and spineless (ss) genes to control differentiation of distal antennal structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02126890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK19"/>
    <dbReference type="STRING" id="36166.T1GK19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003830-RA">
        <property type="protein sequence ID" value="MESCA003830-PA"/>
        <property type="gene ID" value="MESCA003830"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_405596_0_0_1"/>
    <dbReference type="OMA" id="HMNIRMG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007469">
        <property type="term" value="P:antennal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021556">
        <property type="term" value="P:central nervous system formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048749">
        <property type="term" value="P:compound eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007379">
        <property type="term" value="P:segment specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000293">
        <property type="entry name" value="Tigger transposable element-derived protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051839">
        <property type="entry name" value="RD_transcriptional_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215:SF13">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04218">
        <property type="entry name" value="CENP-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50960">
        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH psq-type" evidence="11">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="9">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="414"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="440"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003830-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="567" mass="61890" checksum="EB6C73E1AD1D0E33" modified="2013-10-16" version="1">MNIRMTSKGKRPLRSLTPCDKIQAIQRIHDGESKASVARDIGVPESTLRGWCKNEDKLRFMSRQSTADKMSADSLTEKLCDGAAAAAAALLGVGPPSEKRQKLDQKIPLNFSNKMNYEDLAYKRSPLNGLDFSGSKGLPDLGFNTLSSDYNPFKTAADLSINGNNNVKNKAISPLSSLSHLSGLTGLSHSPLALSFNELTSNLNLIAQLNPSLAAMSGLNGLNNSASALRNVKPKMQPSPQQHSPRSDCDKPQQLQVKNWAKQRNDYGLNLTTDNKNKMKSPSPSLAPSISVNNAGPMDDPLFYWLKSQQAMLGLNNLYPSNPNSSSPPLRSSTPQQPFNPPLSASPPPLSSTSHLNNIDDAKNAAWLNWCKAFGASINSLNAQGNPLLPPSSQQMDGKQAQFENILYSHLTKDSIGNDVNDNEIDVNGNTKPEPEDLSAKSSTKTPSPRSSPKLEPDTVTSNPKEQLDNILYKIHQNTNDNESDVNEEEKVSSAVACDTYSDSEDLEGLIDALEHGEKFLKWLENCSNPRVTAVQLMQLRFLISSIKSGIDKDDEGEPKSKIRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK46</accession>
    <name>T1GK46_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02086957">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02086958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK46"/>
    <dbReference type="STRING" id="36166.T1GK46"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003861-RA">
        <property type="protein sequence ID" value="MESCA003861-PA"/>
        <property type="gene ID" value="MESCA003861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_465616_0_0_1"/>
    <dbReference type="OMA" id="SCIQGRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000002">
        <property type="entry name" value="Multiple epidermal growth factor-like domains 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042635">
        <property type="entry name" value="MEGF10/SREC1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043:SF8">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="211"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="535"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="586" mass="65435" checksum="F2F93483D7CAB5A4" modified="2013-10-16" version="1">MCHPWTGECICKPGWTSQQCNRPCPLLKYGKKCEHTCDCKSGSKCSPVDGKCICWPGLYGEKCELTCPEGKYGQDCKENCDCQNGATCQGETGRCLCKPGWKNYKCDRPCDKNTYGHECGKTCECHNGAACNPENGECLCAAGWTGKLCDKKCEPGFYGHGCELKCECNMTNTKACEATNGTCHCINDWGGYIGPNCEHACPVLYFGQDCRQRCQCANGGECNHITGECKCQPGWTGSNCSLACPDNSYGPNCVHKCLCKHSKGCRKNDGLCICLKGWTGSTCEDICPEGFFGDHCLDSCNCPNQNFICHPADGCVCRRGFRGDNCDQNASEQFVDDHERLYSETSSAGLTWGIVLALMLVGVIIAVAFYYRRKVSNLKTEMQHVQYITDPHHPDRHNFDNPVYGYPGSDSAHLINGVRPKLNNLDRSNGNGLIRDWDDNSNASSRGERFFFQMFKNWTYSVQYNSDILQKNMNADMTNPNTYHTIEPADHVYAEINQKEGYKADPDEYDHLDYSRPSHSKPQYHKMNDTVLNINSDEEKPSNHKNIDVLPKIPENGDAKLTNGQKQFKETDSDDDEEEVEGAKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK56</accession>
    <name>T1GK56_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02143558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003872-RA">
        <property type="protein sequence ID" value="MESCA003872-PA"/>
        <property type="gene ID" value="MESCA003872"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485917_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003872-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9654" checksum="FCEE9C0B84DA1CD6" modified="2013-10-16" version="1">MLLFSSLNPSSSEETVPNSSEFLFCPISDSIYSIETKLSLKKYFLNFCLKFPSQQKILKLLNRASARRGVAPVRRGGGGGMIKLQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKM1</accession>
    <name>T1GKM1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004048-RA">
        <property type="protein sequence ID" value="MESCA004048-PA"/>
        <property type="gene ID" value="MESCA004048"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640970_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004048-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8710" checksum="CA8D0129F107F945" modified="2013-10-16" version="1">MKPNHVPYRTVNDMMLVLEDFSAQMGNESIYIGKIGRNTTTNRNDSDGIPAELLKTAGSSFNRASHLLLNRIWMEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKM2</accession>
    <name>T1GKM2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metalloendopeptidase</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CAQQ02192137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKM2"/>
    <dbReference type="STRING" id="36166.T1GKM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004049-RA">
        <property type="protein sequence ID" value="MESCA004049-PA"/>
        <property type="gene ID" value="MESCA004049"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017286_2_3_1"/>
    <dbReference type="OMA" id="GAQRNIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF814">
        <property type="entry name" value="MEPRIN A SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="12">
        <location>
            <begin position="52"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004049-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="28039" checksum="8BD1ED04332F30F4" modified="2013-10-16" version="1">SLYSTIKSSPRSSLIKTNQDEIPNSEFDLTEGDMILTPRQKEILYSVSDTRNVAKNPSLKWPNGIVHYQFASSIPSKTRDLVLTALHEIEKATCVRFREGANSENNYILVTNNVKGCFSHVGYGRYVQRLNLSNGCLRKGKIIHEFLHAIGFYHQQSSSNRDDFVEIHIENVKSGHEHNFKKYSSSKVTNFGLQYDYESIMHYGPYTFSVNGEPTIVAKQEGAENMGQRFGLSKSDVLKINRLYGCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKP3</accession>
    <name>T1GKP3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKP3"/>
    <dbReference type="STRING" id="36166.T1GKP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004070-RA">
        <property type="protein sequence ID" value="MESCA004070-PA"/>
        <property type="gene ID" value="MESCA004070"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906666_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004070-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7402" checksum="0E657BAF5AC8BBE3" modified="2013-10-16" version="1">MKFKKNVKKEQETSFHNSFSHQKTPLSKELHQKYKIQSMLIHKDHEVCGHFKGNQTCKVVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKW3</accession>
    <name>T1GKW3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKW3"/>
    <dbReference type="STRING" id="36166.T1GKW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004144-RA">
        <property type="protein sequence ID" value="MESCA004144-PA"/>
        <property type="gene ID" value="MESCA004144"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019533_2_0_1"/>
    <dbReference type="OMA" id="FMGRREN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF60">
        <property type="entry name" value="GRAM-NEGATIVE BACTERIA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH16" evidence="1">
        <location>
            <begin position="13"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="33392" checksum="75B8C655051A1C93" modified="2013-10-16" version="1">MIFEETFTNLNSSIWQNVIQIPSDSYDSDFVTFMGRRENIYVSAGNLHIVPSLLTDIPGYRSVQRGQMNLGKDCTPTSDFEKECYRQPNGYRILSPVVSGKIRTKDSFSFKYGRVVVRAKMPKGDWIFPLITLEPTAFNYGATITLLDKLELLILEEMKNLSDQRDFLKSKRNNEHYGNQFYNFELLWTPKSLRFYVDDVQYGEIKPNNLANTNIKHASIWSTGEELAPFDKEFHLSLGLSVGGVSDFPDSSLNGVPRRRKPWANDDPKAELNFWKKTSEWLQTWDGVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKY5</accession>
    <name>T1GKY5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKY5"/>
    <dbReference type="STRING" id="36166.T1GKY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004168-RA">
        <property type="protein sequence ID" value="MESCA004168-PA"/>
        <property type="gene ID" value="MESCA004168"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1158885_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026720">
        <property type="entry name" value="CFAP91"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455:SF10">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="28141" checksum="FD0A4AB7BDE081D9" modified="2013-10-16" version="1">EFRKSSRRPTEALKCVKNIPTTKTVELKTPSTKSGLQYYTTSKMNLSRQSKVALNEQHDEHIAHSEVFKDSILGAVQFKEERALDEIIKHEDIVNSIFAQYEGEKIGWIMQFLEEELDRLHEERRVQAFAMLAEKERRKLEAAEAGLRQKENIRREEQEYFFQNIQNSNISTVNEYLENVILEEYELTASNDTTKYIENVAKMIAMESRMESSKKFDPKVLQESVSDLLIPAIYKRLERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL81</accession>
    <name>T1GL81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL81"/>
    <dbReference type="STRING" id="36166.T1GL81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004276-RA">
        <property type="protein sequence ID" value="MESCA004276-PA"/>
        <property type="gene ID" value="MESCA004276"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847401_0_0_1"/>
    <dbReference type="OMA" id="VIPCNIY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050577">
        <property type="term" value="F:GDP-L-fucose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43238">
        <property type="entry name" value="GDP-L-FUCOSE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43238:SF1">
        <property type="entry name" value="GDP-L-FUCOSE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004276-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="16112" checksum="CCFDAD7E9444E98D" modified="2013-10-16" version="1">MKISLSYMTLFSRDNILINDNVLQTAFENKCEKVVSCLSTCIFPDKTPYPIDESMIHNGPPHDSNFGYSYAKRLIDIQNRAYFEKHGSMFTSVIPCNIYGPNDNYNPEESHVIPGMIYRMHRLITENPEIPEEEKVFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLN0</accession>
    <name>T1GLN0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123008">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004442-RA">
        <property type="protein sequence ID" value="MESCA004442-PA"/>
        <property type="gene ID" value="MESCA004442"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1995206_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004442-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14811" checksum="85587CBA82DD5D55" modified="2013-10-16" version="1">MADDIMTAFPKTRSQSKLLSKLAEWVESIRFGWFLKYIGFPEADNLIDNLVSREILSFAKKNWNQCAIELALLDYFYKHVGNVRNVWGFGNILVRNNIFTKTLRSSFPNRHTFFALRHYKKKHEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW3</accession>
    <name>T1GLW3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial import receptor subunit TOM20 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02098497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLW3"/>
    <dbReference type="STRING" id="36166.T1GLW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004528-RA">
        <property type="protein sequence ID" value="MESCA004528-PA"/>
        <property type="gene ID" value="MESCA004528"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100000_1_1_1"/>
    <dbReference type="OMA" id="DMIAYDG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022422">
        <property type="entry name" value="MAS20_rcpt_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01989">
        <property type="entry name" value="EUOM20RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004528-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19023" checksum="DD90449BD646536B" modified="2013-10-16" version="1">MIEINKTTIGLAAGVAGTLFLGYCIYFDKKRRSDPEYKKKVRERRRRQRKNGGGGRSGMPNLNDHEAIERYFLEEIQLGETLIGRGDFENGVEHLANAIVVCGQPTRLLQVLQSSLPAQVFGMLIQKMQDFSKRAEAEAGPRIVTVQSSAPLNVDNELDMSINEVIDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMQ0</accession>
    <name>T1GMQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02006208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006209">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMQ0"/>
    <dbReference type="STRING" id="36166.T1GMQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004828-RA">
        <property type="protein sequence ID" value="MESCA004828-PA"/>
        <property type="gene ID" value="MESCA004828"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="OMA" id="FGLMLEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046701">
        <property type="term" value="P:insecticide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046680">
        <property type="term" value="P:response to DDT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000042">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF45">
        <property type="entry name" value="CYTOCHROME P450 6G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56940" checksum="2F3434E0178015D5" modified="2013-10-16" version="1">MQRLKNNQSAGSNGIPTELLRQQELPLTMSFTTYWQTSGLVNRSLDLYLQDKSPGHCIPYGFDEENSGFMFQDLYEDKVAKNEPYVGIHIFNKPALLIRDLELVKKVLIKDFQYFSNRFTRMNPHNDPLGSNNLFLIRNPMWKELRQKLTPVFTSGKMKQMFHNVDEIGLKLNEHLLSLPMDKQNGKATCKELKELCALFTTDVIASVAYGLQANSMKNPEGEFRKNGRKLFTYTVGRSIDFTAFFFLPQIIPLVNATLFTKEASKFLFSTIGYAMEEREKSGTVRNDLIDVLLTFKKQNYPHDMIVAQAAVFFTAGYETTSSTMSFALYELAQNQEIQKRLRNEIKEALIKSDGKITYELVHSLEYLGMVVQEVLRMYPPLPFLDRECAVDNPKDSYKLDDKFNPQYFPKPNTFNPERFAPGNEPGIANMPFGIGPHNCIGERFGLMQAKIGLINFLKNHYVAPNEKTQKEMVLDPLALIIAAKGGIYCDIVRDPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN31</accession>
    <name>T1GN31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl hydrolase family 13 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN31"/>
    <dbReference type="STRING" id="36166.T1GN31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004970-RA">
        <property type="protein sequence ID" value="MESCA004970-PA"/>
        <property type="gene ID" value="MESCA004970"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122084_0_0_1"/>
    <dbReference type="OMA" id="EHINVAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF233">
        <property type="entry name" value="MALTASE A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004970-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16321" checksum="ECF903E6FA2FC0F5" modified="2013-10-16" version="1">MLLQTLPGIGVTYYGEEIAMENKYIDWEHTVDTQACNADPEKFDDYSRDPARTPFQWDASTSAGFSTNKDTWLPVHENYKKSNVLLQRRAPVSHLQVFKKLIKVRREPSFQEGTCERKVIQQDNILIYKREKAGSDKYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN61</accession>
    <name>T1GN61_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN61"/>
    <dbReference type="STRING" id="36166.T1GN61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005004-RA">
        <property type="protein sequence ID" value="MESCA005004-PA"/>
        <property type="gene ID" value="MESCA005004"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_59_1_1"/>
    <dbReference type="OMA" id="TSAHHIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001955">
        <property type="entry name" value="Jim, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000683">
        <property type="entry name" value="Zinc finger protein 252"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000505">
        <property type="entry name" value="zinc finger protein 32 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
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        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
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    </dbReference>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
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        </location>
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            <begin position="600"/>
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            <begin position="86"/>
            <end position="105"/>
        </location>
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    <evidence type="ECO:0000256" key="6">
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005004-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
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    </evidence>
    <sequence length="668" mass="75138" checksum="504119ED9A3F87A0" modified="2013-10-16" version="1">MALNFSASFAACLAAGLKVPRQIMYCISQDTGAPYVLYKDTPETGDRQGTAAIGQPAQWSTEVQRNLNQIHIIILKVTIQQQNVQNGQQPQNTNQQNQSNEQQSQRTIEITVNNPQASSQQQQQINANGQPQQQPNGTFNSTQAEISYYAPRHPNAHPQGTMPPTMLASSGFTPLHYINKPLEQNGPSGGSNIETYTMSDLLPAHNGVLHASNTNSSPSSNNTHKSKSHNNHSSSDLRLFKCLTCGKDFKQKSTLLQHDRIHTDARPFPCLECGKRFRQQSHLTQHLRIHANEKPFTCAYCPRSFRQRAILNQHIRIHSDVSPHLIFKNGPHPTLWPQDVPFPGEETDNKNDIAAYGGDDPNLIQHGRAAGMPLYVRCPICNKEFKQKTTLLQHGCIHIESRPYPCPECGKRFRQQSHLTQHLRIHTNEKPFGCLYCPRFFRQRTILNQHVKIHTNEKAFGCSYCPRFFRQRTILNQHVRIHTGEKPYKCGQCGKDFRQKAILDQHTRTHQVGDRPFCCPMPNCRRRFATEQEVKKHIDNHMSPNSSKSRRPPAPPAQQVQSDNSKHNVTAAAAAQFITALDNKTNIIPRMVPQPITSILATNIHPPNPASSTTQLHPQTQQQQQQPQPTPPQQQHHHQPPAPSATAVSAAATTIQIHPHIHSIFGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN63</accession>
    <name>T1GN63_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005006-RA">
        <property type="protein sequence ID" value="MESCA005006-PA"/>
        <property type="gene ID" value="MESCA005006"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005006-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="296" mass="34868" checksum="E9F44C887311A81F" modified="2013-10-16" version="1">MLSAYVKGRQALQDLENVKDLKIKDKLRNFVVQSIVQFGYENRLKFSRENINSFSEEIIQIFPTEKKDFYFIPAPPSILLENMQLQKFSPKGKLYDSYNNSMKCDIYHPKKKNKSDVANPLDEEASTVEEVPRGQYYLKTWLSQTQANKSMFESRWRDTFGLREMELVPKIEIFLKGWPAFSFSFGCELLDMDCFELLADKNNGLIENYSNIFNTALQKAFEKSKDKINRFNPSKERNTNEFVLDSLYTLSYLCRLGRKLNYEQVIRSMVYIGDNFDYRESKYPIIVINNDFSIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNB6</accession>
    <name>T1GNB6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="36166.T1GNB6"/>
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        <property type="protein sequence ID" value="MESCA005062-PA"/>
        <property type="gene ID" value="MESCA005062"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690638_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005062-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8396" checksum="5003E9681D6E0648" modified="2013-10-16" version="1">MNLQNLNKLGSSHLITVDFSHNIISEINLETFDGLENLKFLDLSFNKISKIPSRILEGIQNLETLNLSHNCLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQF0</accession>
    <name>T1GQF0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heme peroxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQF0"/>
    <dbReference type="STRING" id="36166.T1GQF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005855-RA">
        <property type="protein sequence ID" value="MESCA005855-PA"/>
        <property type="gene ID" value="MESCA005855"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_928163_0_0_1"/>
    <dbReference type="OMA" id="CERNICY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF125">
        <property type="entry name" value="GH11385P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005855-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28094" checksum="27F9F2B475D19C33" modified="2013-10-16" version="1">MGMRCCSEDYQHALPNTQSHHSCLPFEIPPGDRYFMQMNPQPRCHNFIRTQPIFHDNCTVSAAEQVNMPSHFIDLSVIYPLTMEKLKSLRMFSGGLFKLDEKMIMVKMENCEANCFFAGDFRAAGFASLAVVHSIFMRLHNMLAMQLAKVNPQWNDDMLFFEARKITIGMYQHIVYNEYIPSMLGQTSFAVAGDGDYDKNMDPRTLNEFSNTAFRYLHIYTPDVINLYNDKMQVTMSSAISNVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQF2</accession>
    <name>T1GQF2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02016704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQF2"/>
    <dbReference type="STRING" id="36166.T1GQF2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005857-RA">
        <property type="protein sequence ID" value="MESCA005857-PA"/>
        <property type="gene ID" value="MESCA005857"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877781_0_0_1"/>
    <dbReference type="OMA" id="PAMFECW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF424">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="63"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005857-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15314" checksum="696534059364E1DC" modified="2013-10-16" version="1">MLNYPSISYNGFQDSQLAPSVKNASCSNSRYLLSEHSFIFYIGFGEIVEASIILILTLGVISANMLVIIVINSRKYSSYIHQQPRYLLTSLALNDLAVGFLITPFGILPALIHCWPYGEIFCQIQVCMKHYIKCNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQK8</accession>
    <name>T1GQK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein hedgehog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product. Once cleaved, the C-product has no signaling activity and diffuses from the cell.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the hedgehog family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02106290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQK8"/>
    <dbReference type="STRING" id="36166.T1GQK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005917-RA">
        <property type="protein sequence ID" value="MESCA005917-PA"/>
        <property type="gene ID" value="MESCA005917"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576590_0_0_1"/>
    <dbReference type="OMA" id="RTADKNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016015">
        <property type="term" value="F:morphogen activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005113">
        <property type="term" value="F:patched binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007367">
        <property type="term" value="P:segment polarity determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001657">
        <property type="entry name" value="Hedgehog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050387">
        <property type="entry name" value="Hedgehog_Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000320">
        <property type="entry name" value="Hedgehog_signalling_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Zn_M74/Hedgehog-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11889">
        <property type="entry name" value="HEDGEHOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11889:SF31">
        <property type="entry name" value="PROTEIN HEDGEHOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01085">
        <property type="entry name" value="HH_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00632">
        <property type="entry name" value="SONICHHOG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="Hedgehog/DD-peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0504">Morphogen</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0709">Segmentation polarity protein</keyword>
    <feature type="domain" description="Hedgehog N-terminal signalling" evidence="18">
        <location>
            <begin position="10"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005917-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9466" checksum="79D8D324E3C6BF28" modified="2013-10-16" version="1">MFQTQERFDHIAKRTADKNCKEKLNNLAISVVNEWPELKLRVIESWDEDLQHGEHSLHYEGRAVDITTSDRDRAKNGMLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQQ7</accession>
    <name>T1GQQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQQ7"/>
    <dbReference type="STRING" id="36166.T1GQQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005970-RA">
        <property type="protein sequence ID" value="MESCA005970-PA"/>
        <property type="gene ID" value="MESCA005970"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782105_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005970-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="17333" checksum="D30FE183C5505C8E" modified="2013-10-16" version="1">RQLSKEDPFNQETVLDDTSITEISEKDIFEKHEICESVDDTAEIEPFKPKTILKLREFHKGDLVRYGERFKKTGLRWRKATIVEKITSISYKLNINGEDKTVHVGFLKRDNNQKVNFGGKEYLEIDYEQLEEEEEAERNYSIWDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQV2</accession>
    <name>T1GQV2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006017-RA">
        <property type="protein sequence ID" value="MESCA006017-PA"/>
        <property type="gene ID" value="MESCA006017"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256780_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kazal-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006017-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12218" checksum="5EABE2133E54F70F" modified="2013-10-16" version="1">AKAKSVDEKCQIECFDDENPVCGTISRVCYKTFKSKCALEKYNCSHNGVYDLFYEDKCDQRLYSCDQQRDECEMNPCPLHIRPVCGFNGECYKTFSNSCFMEKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR81</accession>
    <name>T1GR81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aralkylamine N-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.87</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dopamine + (9Z)-octadecenoyl-CoA = N-(9Z-octadecanoyl)-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51380"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31883"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:51381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dopamine + acetyl-CoA = N-acetyldopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51388"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:125678"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:51389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dopamine + hexadecanoyl-CoA = N-hexadecanoyl-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51376"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:134058"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:51377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>serotonin + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = N-[(5Z,8Z,11Z,14Z)-eicosatetraenoyl]-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:132255"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:51397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>serotonin + (9Z)-octadecenoyl-CoA = N-(9Z-octadecenoyl)-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51392"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:134064"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:51393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>serotonin + acetyl-CoA = N-acetylserotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25217"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17697"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
            <dbReference type="EC" id="2.3.1.87"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:25218"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>serotonin + hexadecanoyl-CoA = N-hexadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51384"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:134059"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:51385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>serotonin + octadecanoyl-CoA = N-octadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:134065"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:51401"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the acetyltransferase family. AANAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.87" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02117984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02117985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR81"/>
    <dbReference type="STRING" id="36166.T1GR81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006157-RA">
        <property type="protein sequence ID" value="MESCA006157-PA"/>
        <property type="gene ID" value="MESCA006157"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654139_0_0_1"/>
    <dbReference type="OMA" id="CLEVEEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000046">
        <property type="entry name" value="Dopamine N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905:SF1">
        <property type="entry name" value="AT07410P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905">
        <property type="entry name" value="N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006157-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="18302" checksum="6F7AA4C0BC6754C1" modified="2013-10-16" version="1">MEFFITQILETPMWTVSVTGHEEESPSLASQVEHPKFKKIMQLMDYIDANFNIFDVYPNANTILDGKILSVNTNYRGLGIAGVLTEHTLNYMRQHDIPVMHVLCSSHYSARVVEKMGFHKVYELKYSDYKVNGENPLLPDEPHVAAKIMVKEVNNTNSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR86</accession>
    <name>T1GR86_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR86"/>
    <dbReference type="STRING" id="36166.T1GR86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006163-RA">
        <property type="protein sequence ID" value="MESCA006163-PA"/>
        <property type="gene ID" value="MESCA006163"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1201025_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="231" mass="26302" checksum="F1EE28350EB0083F" modified="2013-10-16" version="1">MYTTITQLVREQVYKFYCVDNITAFMESNVASKNSVNSSTFDFSRPHYVKTTAWGGQIYRLIAIFEDGKGSEQIKPSSIPTFSGREKDKNRYAQLLNENRQVLYVSLSTKGKFYEIEQSVPQMIQRDSISSARGISSATSQRKINQDCVYKLSTLIHGLSKETPLDLKYIGGHHSDQSIPENLTIFKISTEDICVICSMPNESFIQQQQTTIQPISELRKINITNNLLFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRH6</accession>
    <name>T1GRH6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006261-RA">
        <property type="protein sequence ID" value="MESCA006261-PA"/>
        <property type="gene ID" value="MESCA006261"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592505_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9389" checksum="4DCDD54B4F4385AB" modified="2013-10-16" version="1">MCFYNVQHDASDMNTERNSGLSTLPQYVFNIFSKYKWIVTVDQFLRIESNSFYAVYPLCKADALHTKLRALPLVNYLLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRJ5</accession>
    <name>T1GRJ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006280-RA">
        <property type="protein sequence ID" value="MESCA006280-PA"/>
        <property type="gene ID" value="MESCA006280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870190_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7531" checksum="43280D63D7E8D52C" modified="2013-10-16" version="1">MSSVYAAASTPHLPHFIENYLDHRKERRHNRGMERQHNMSCPVHKKGHRSHCKKHIDSTTDSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRL3</accession>
    <name>T1GRL3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144745">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144746">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRL3"/>
    <dbReference type="STRING" id="36166.T1GRL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006299-RA">
        <property type="protein sequence ID" value="MESCA006299-PA"/>
        <property type="gene ID" value="MESCA006299"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_16_4_1"/>
    <dbReference type="OMA" id="HCEEARE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006299-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="20065" checksum="F5473DF8C1F470E8" modified="2013-10-16" version="1">ASYVITTKIYWSTKSEERGLSRKHIIECVKASLQRLQLCYIDIVIIHKADAMCPMEEVVRAMNYVIQQGWAMYWGTARWTSVEIMEAYSNCRQFNCITPICEQSEYHMFCREKVELYLPEMYNKIGVGLMAWGPLSMALSDTQNNEKILFPKGSFKTKSHSYSWTEDDTNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRR3</accession>
    <name>T1GRR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Battenin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lysosome membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02102129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GRR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006353-RA">
        <property type="protein sequence ID" value="MESCA006353-PA"/>
        <property type="gene ID" value="MESCA006353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1403924_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="23179" checksum="36C017D4C9AF9ACC" modified="2013-10-16" version="1">MLVMLAFPAIEAFAFWVILRKPTELYDNNYQDSKDDSESTRLTWPDEEPLVTWKDKFFYTGHLMKYLIPLTTVYLFEYFINQGMFELVFFENISFLDKAAQYRWLQVDYQIGLFVSRSSVNLVTIDKLWVMSVLQFINVVYFLFEVIFFFTPSIWIVFLIVFWEGLLGGGAYVNTFYKMSKEVYCRXILDGYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSL4</accession>
    <name>T1GSL4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSL4"/>
    <dbReference type="STRING" id="36166.T1GSL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006674-RA">
        <property type="protein sequence ID" value="MESCA006674-PA"/>
        <property type="gene ID" value="MESCA006674"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362099_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006674-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11109" checksum="66A91473C11BEE1C" modified="2013-10-16" version="1">MYILYSEVGYTMRDIRYFWRDGLSSVGMSKEVELPQFRVLGHKQTAKEINLSTGTYPHFQINCTLRMGGFGSMGHLVALRLYNFILLAKKKILMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTH1</accession>
    <name>T1GTH1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02200191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GTH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007003-RA">
        <property type="protein sequence ID" value="MESCA007003-PA"/>
        <property type="gene ID" value="MESCA007003"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_229103_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="878"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1470"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1908"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1949"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1989"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2159"/>
            <end position="2189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2223"/>
            <end position="2250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1045"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1535"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1583"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1601"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1619"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1908"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1950"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1998"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2170"/>
            <end position="2179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2226"/>
            <end position="2250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007003-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="2422" mass="267339" checksum="4DE58DE547B3FD67" modified="2013-10-16" version="1">MFLIVTSMSKEXXXXXXXXXXXXXXXXXXXXXXXXGSNSHQYSSEQGAIETATKSSINVLYIESDKSSTEQQAIETSTNSIQIPDSASSTEQGIIDTASRSTSYVLNSDSNEYSSEQSITETPSKDFVQILGNEANESSKNTTQTLSNESKESTTDSIYSHMSREEILADVKGTPISFINSMIDLEEKIETTSSKNDFTSADQDDTLMHLLGISVSDVNSMDTIGIDITTTENSIFVQQSSKSIPSEKEDTFSINQEDTIVPTTQNIIDEFSSSPKLVNSMKTYEEEQFTLRPEIEDTVDKYVKGLPGIFGDEFMDSFDYEEESAKNDEIHNQTYKLIDESIGSEYILYENKSQKDINIEAEVSTEKSNFIDESIGTTENYLMRGSLENTEGYLENSAYVTTENPLVEEVKTTISVTRNDNIDEHVEITTENIRLIEENSHSSVLGTVELRTSSEASIVENANVAMNKSEIFETSSESVKNQVHETSTTIKTNSEQGMSRDEVESIESTTLLPSNIISEVREHEVPFDVMESSANEKSPEKSEEFVESTNKFIDSNERKVSKTDNFETSEYESEKIDKIVNSTTIQNAHMQDSEREDSFVESTTIREIRVSFPEKSSPEEVESTTQGSLLHVKETEMSHSIKVHPKVKAEVTTTPIPAGKSYLLHKIENSEYSNIQKIPSRNTNVFATSTESNENDKFEVFGEIYSEIKSTSSDSAIVTGVSSDEAIKKVQEYQHSSPDETINFDIHKSSSEVQESLDNSQTDIPSSNVQDIKTQYIENSSLGSQELPNIHNDQKFAQENESKTTSSMLSEEIIHQALPKIDSEQKFDLTEEVSSEINHYDNSEVVQELPNMNYEQKFHDQNDQKMDLFVASNQLKTTEDSFNSQKSSSDSSSSSSITKKEDSSETSSHTSITNQQLPTKDIQQNFYGSMESESNLSQTSNQNLKDMIRENLNNNSQNSNQSFEVKVNYNEEANKIKKLKNEFESMIKSELNEEQNSEIDSYISLIPLYMRNRNSSISSGFVTQRVEGNSNGTTLDNGFIPPSDEGNSNGASLESRVQSNENKSSGFVSQSVESNINGASFESSDQEIGQNYGDQKISDVLENKSSGLANQKEAQIISSIKTEASLENTAQNYDDQKISFVLNSANLENKSNNFATQIVDSLKTESSLSQTNDDTKTESANIQSQNTQEFLNNVKAHMEKNSQNSNFDSKYYDNIIKAWIASRESSTQSSQILDSEENILAKEKLKEFDSKIQEDIDHIINEVKILQSQIDSEKAEPSFTQINSNSEGNLEFVKVKGGSNIEHSIEKVESSTSGSSSIVQAHVNSNEEISTNKFNLSNNEGNLLVVQGQGSLNAESSTINPSLDHHFSEIHSNNEGNVHFVQGQGSLSAESSTIMPETNKPSLHHNVEPVKESIQTILDHNVTSTETIKTSLDHHFTDIHSKNEDNLQVVQSQGTLSSESSIANVETINSNSNNEGNVHIAQSQGSLSAESSTVIPETIKTSLDHNVETSTETIKSSLDQHFTDIHSKNEDKLQVVQSQGTLSSEAKVESINSNSQGNSEFAKVQETSNTESSTGSIQSNTYESLSDNSKSESNSEIGVSGASNIETSTSKPKFGSKPSTSTTVITSQVTGNEPSTSKESVIIHEVTSNKKSEEASFNSEENLKINKFTTHKPLLPKDTPLIVKITEGNPSFSVEIPESDSVVSHIKPQQVFDNIESQNFLKENKLSSQIDLDQIPKNKPSEFSVSYSTSVSKTQVPSAQKPTISKKAQPFTIQSDDKFDSLERQANILTNFHDLLVERNFAPSQYVASKVPIVLQMTKGKGKSIPEGSLVFRSGNEIESEYDDNSAPSTLTQKEIQQTQKLIKAKTTKDLAYFLHEENLSISEIPKPVLVNAIRTLTAIVNTLESSTTTTTTTTTTTTPSTTATTTKTTTTMRPAKLHRFPRLQRELYTTATASTTSTQKSKDRQPSATVERNLELLQELLSLQAKIEKQNSRKTTHTPGTTASTTTETTTRMTTTTTTTTPEPTTTRRITTKGTKSTYSDAEDLAFLENLKKAIGQQRTTKTTTTSTQSPTTYMEDLYNSFQSATPVTPKKSTFSDQEDLEFLNNLRQFAKFNVTTPQSPTFANRKDEAQLGQDLALLSSLLGRRISAEDIPKLTQQYQPKPRQPVHTTTTTTTTTTKSTQRPMSKDELAEVEKNLKLLSSILGRPISIEELPTIMKQYRFGRNPTAPTTTTTTTTTTPSPPIINSNTPITRDEVIELLKDPDVGLGDNIDTYGKTNDAILAAVLKQKGIGPAHNNVPVDDLFLQGPTATPYVTTTDLRDQGDPSSLNNSSNKEAIPYSADCLGFGRRGKRLPQSDDFALEPEPAETPTAYPQGGGLLAAAFGVTKAVTQFLGAAFQGAAKTVQQSWNTGDLDNPFATSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV06</accession>
    <name>T1GV06_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007580-RA">
        <property type="protein sequence ID" value="MESCA007580-PA"/>
        <property type="gene ID" value="MESCA007580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2352602_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588551" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="10928" checksum="2370C7BBDB2A1663" modified="2013-10-16" version="1">MLRLKIIKSYFILFFILFTIGCIQGLENDLEPKIVKSSNSEVVFSNGGDLAIFCIAHGFPRPSIQWFRNGEHYEGSISESQIGNNVIKSMIEIEDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV43</accession>
    <name>T1GV43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Charged multivesicular body protein 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV43"/>
    <dbReference type="STRING" id="36166.T1GV43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007625-RA">
        <property type="protein sequence ID" value="MESCA007625-PA"/>
        <property type="gene ID" value="MESCA007625"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080826_0_0_1"/>
    <dbReference type="OMA" id="QQITMVM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007625-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20604" checksum="7BF25DB9E0799EA0" modified="2013-10-16" version="1">AVKELERSSKKCEKDEKLEKAKAKKAIQKGNMDVARIHAENAIRQKNQSLNYLRMSARVDAVASRVQSALTTRKVTGSMAGVVKAMDAAMKGMNLEKISGLMDKFEQQFEDLDVQSSCMENAMSQTTTTSVPQGDVENLLQQVADEAGLELNMELPSGPQSTIVGASTQASQEQDELSQRLARLRQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV49</accession>
    <name>T1GV49_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sodium channel protein Nach</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02149202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV49"/>
    <dbReference type="STRING" id="36166.T1GV49"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007631-RA">
        <property type="protein sequence ID" value="MESCA007631-PA"/>
        <property type="gene ID" value="MESCA007631"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020415_1_3_1"/>
    <dbReference type="OMA" id="QIGMENG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF237">
        <property type="entry name" value="PICKPOCKET 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007631-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="36420" checksum="8591030F22967A0B" modified="2013-10-16" version="1">MPSLSTSKRTMKRNSPKADIFTITRSAKVKKRLIKDDTSLKSIFIKHFRNYCDNSSLHGVYYITKPGLSKWERRFWVTVVCLSLLSAVILVWVNWIQSQKTRTVTLLETTFYPTSNVNFPAITLCNFNKISKKKALELAKTLKKPSSINDTELANLFKVTINYSFGMRGNNTEFQILDSILKTNEMSWDGLLSYVQPNCLDMAIKCFWKGSDTRCDGLFQTINSSEGTCCSFNYYGLEKNNYPTKKLQYTVPKRPYRITGSGFQTGLSVLLDPLLDDYFGSFFDSIGFRVLIHDSYDFPDENAETKMLNFGRETFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB0</accession>
    <name>T1GVB0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proliferation-associated protein 2G4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB0"/>
    <dbReference type="STRING" id="36166.T1GVB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007701-RA">
        <property type="protein sequence ID" value="MESCA007701-PA"/>
        <property type="gene ID" value="MESCA007701"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041451_0_0_1"/>
    <dbReference type="OMA" id="CAKHELW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000029">
        <property type="entry name" value="Proliferation-associated 2G4, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007701-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24247" checksum="218A86543241982F" modified="2013-10-16" version="1">MGTNYLVTEAVQKVAESYKCKPIEGMLSHQLKQFKIDGEKTIIQNPSEQQRKEHEKCNFETHEVYAIDVIISTGEGIGREKDTKVSIYKKTEENYQLKLKASRSFFAEVKSKHGSMPFNLRQFEEETKARMGVVECVSHKMIDPFQVFPINAKGINLVTGIPFEEDVYVSEHSVTDEKIKELLATPINPSKASKGKKGPKKSATASDPAPAAVPTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVJ4</accession>
    <name>T1GVJ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVJ4"/>
    <dbReference type="STRING" id="36166.T1GVJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007803-RA">
        <property type="protein sequence ID" value="MESCA007803-PA"/>
        <property type="gene ID" value="MESCA007803"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461106_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20901">
        <property type="entry name" value="CC_AF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049773">
        <property type="entry name" value="AF10-like_CC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007803-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10251" checksum="6A126AF9256981A5" modified="2013-10-16" version="1">MLADNGPNGSHPQTLEELLERQWENGSRFLMDQAQHFDIATLLSCLYELRTENSRLEDHVNSLKARRDHLLALNARLQIPLSQTAQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVP3</accession>
    <name>T1GVP3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVP3"/>
    <dbReference type="STRING" id="36166.T1GVP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007856-RA">
        <property type="protein sequence ID" value="MESCA007856-PA"/>
        <property type="gene ID" value="MESCA007856"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143070_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF95">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588548" description="Peptidase S1 domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="31"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007856-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="16132" checksum="4B94802DAEA2F2B2" modified="2013-10-16" version="1">MKVAILVLALACGAYSLTLPRFKAAFSKGDIIDGYEAQPVKHLSFHFCAGSIIDESTILTAGHCLAYPASLINVKAGKHLRSSSDGVQSAKAARTIVHPKYNGDVGPNDIGLIKLATPLNLNIRAEGNPVGQINLASGGGGGLFSESKFILETVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVQ9</accession>
    <name>T1GVQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVQ9"/>
    <dbReference type="STRING" id="36166.T1GVQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007875-RA">
        <property type="protein sequence ID" value="MESCA007875-PA"/>
        <property type="gene ID" value="MESCA007875"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_1"/>
    <dbReference type="OMA" id="AHPMEIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002568">
        <property type="entry name" value="Cell division protein (FtsH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007875-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="372" mass="41178" checksum="1366E4D6B976526F" modified="2013-10-16" version="1">MEFVNFLKNPKPYINLGAKIPKGAILSGPPGTATAGEANVPFLSASGSEFMEMFVGVGPGRVREMFAMARKQAPCILFIDEIDAIGRERSEKTHTEEDTTLNQFLVEMDGFKTNSNVVVFAATNRIDILDKALLRPGRFDRQIALVAPDIKGRASIFKVHLTSLKTSLNKNDLSRKMAAMTPGFTGAEIANVCNEAALIAARDSNDSILMTHFEKAIDRVIAGMEKKSNVLSSEEKNIIAHHEAGHAVAGWFLEHVDPLIKVSINPRGKTLGYSQYLPKDQYLHSENQLFDRMCMTLGGRVAEELFFNRITTGAQDDLEKVTENAYSQIVKFGMNKKIGNVCFNTNKQNTKLYSEQTSQLIDEEVRALIDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVT8</accession>
    <name>T1GVT8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVT8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007905-RA">
        <property type="protein sequence ID" value="MESCA007905-PA"/>
        <property type="gene ID" value="MESCA007905"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888354_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007905-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7124" checksum="14C6E1453C3F2173" modified="2013-10-16" version="1">MDLGSDPGFVFPTRVQRASKWKLPDLVGSLLQLNLDTQSYPVKSAFCSSSSQPSEERLHYNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW79</accession>
    <name>T1GW79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LSM1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in the degradation of histone mRNAs, the only eukaryotic mRNAs that are not polyadenylated. Probably also part of an LSm subunits-containing complex involved in the general process of mRNA degradation.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Probably involved with other LSm subunits in the general process of degradation of mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with SLBP; interaction with SLBP occurs when histone mRNA is being rapidly degraded during the S phase. LSm subunits form a heteromer with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW79"/>
    <dbReference type="STRING" id="36166.T1GW79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008051-RA">
        <property type="protein sequence ID" value="MESCA008051-PA"/>
        <property type="gene ID" value="MESCA008051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_0_1_1"/>
    <dbReference type="OMA" id="IGYLRCV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000021">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15676" checksum="B5D00C85F10D98D1" modified="2013-10-16" version="1">MEDLNPLHGTAHLLEEVDKKLMVLLRDGRTLIGYLRSVDQFANLVLHRTIERIHVGNEYGDIPRGVFIIRGENVVLLGEIDKEREQNLPLKEISVEEILDCQRREQEQKQEKHKLISKALKERGLAENNEIAEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXA2</accession>
    <name>T1GXA2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which has been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases such as CDC42 to an N-terminal regulatory domain. PAK1IP1 also binds to the N-terminus of PAK1, and inhibits the specific activation of PAK1 by CDC42. May be involved in ribosomal large subunit assembly.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02158469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXA2"/>
    <dbReference type="STRING" id="36166.T1GXA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008445-RA">
        <property type="protein sequence ID" value="MESCA008445-PA"/>
        <property type="gene ID" value="MESCA008445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031466_2_0_1"/>
    <dbReference type="OMA" id="QGSIKCM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00045213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="244" mass="26769" checksum="07882549B62B3D6F" modified="2013-10-16" version="1">MTQSFADRSHAGSVRSLAVSGKYVASGGADDRIFVYDMKQRNQTQILQHHVGTVNALEFTPDSSHLLSVGSDGHFVATRIGSWMIEGNWRKAHGGSQVTHLTCHPSGKLALTLGSDLILRTWNLVKGRVAYKTNVKGKKTLGHTPDCIHWSTEGNYFTIAGQRCVEIWSIENADVISTIKTTSRPVCICWIDEEHLLVGLENGKILIHSLSEETEDQKTVECEGHSSRVKCLSSNGNVLVSASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXD4</accession>
    <name>T1GXD4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXD4"/>
    <dbReference type="STRING" id="36166.T1GXD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008481-RA">
        <property type="protein sequence ID" value="MESCA008481-PA"/>
        <property type="gene ID" value="MESCA008481"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_656032_0_0_1"/>
    <dbReference type="OMA" id="WERQSER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="6">
        <location>
            <begin position="9"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="7">
        <location>
            <begin position="219"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="436"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008481-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="462" mass="52063" checksum="4FAA5CD79C438C22" modified="2013-10-16" version="1">MFYLGENATKIVCILLTSTLGNKEWNISLPDCEGVVALTATTKYIAVGTDNRFVRFYSVMGTQREVISVPGPIICMAGHKDKVMIAYHSAPANEDQHISIMFVQIFGLNIKCREARLPLPPGRTMSWMGYSTDCGTPVFYDNMGLLQMYSMKGNCWYPMCDTSKHSQSVANNYFIVDVCEKQQIAHAILCRATRYPMTHPRPMVTELPLQMPLFDMTLKDNSEKNIKESAIKLFAMDCRAEKEAKARELVEMIGNPSILELAIKYAGKLGRIHFADRLAEMMPVLQDKESEREKEMQEMEKDAFQILQNTSSLLLMQGCNTPKSDSVKIAPKPMVLSNQKRNPFKRSQNASPQVGSSSPNPFSHLMDKVIEMKPSQDSQEDVIPDSQNIENIIPKRTFVSWFQENKEALQAKHPNASAQELTKLGMTEFRGLSATEKTTKRKLDSDTAGNNMKQAKIDFGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXT9</accession>
    <name>T1GXT9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008645-RA">
        <property type="protein sequence ID" value="MESCA008645-PA"/>
        <property type="gene ID" value="MESCA008645"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729565_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008645-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8452" checksum="4EE2B2786454BCCC" modified="2013-10-16" version="1">SKEILSPWASSQERVGPKINIGHFRGCEKLHLSGNRVYEEGKWRIWYNQELYQLNHHPSVVLILLTRLLQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXZ6</accession>
    <name>T1GXZ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008711-RA">
        <property type="protein sequence ID" value="MESCA008711-PA"/>
        <property type="gene ID" value="MESCA008711"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226193_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12368" checksum="0D339E1F93BD4B1E" modified="2013-10-16" version="1">MDEKSATLVRGALGCVKWEIPTCGPMIKLPFYKPSNLDFFPNFLILQLLLHSKDFFPSYHLYKLLISSYHMPQTLTDFVPTIHLLTLVSRFENPFGFLEDLSWEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY62</accession>
    <name>T1GY62_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Abitram</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Actin-binding transcription modulator</fullName>
        </alternativeName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABITRAM family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02057550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY62"/>
    <dbReference type="STRING" id="36166.T1GY62"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008784-RA">
        <property type="protein sequence ID" value="MESCA008784-PA"/>
        <property type="gene ID" value="MESCA008784"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107323_1_1_1"/>
    <dbReference type="OMA" id="NTSDCAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051489">
        <property type="term" value="P:regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039169">
        <property type="entry name" value="Abitram"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651:SF0">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008784-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13114" checksum="43DD1F33E3BFB0DF" modified="2013-10-16" version="1">MLAPEHEALRVGVKSITFDIGNTNRSQNQVKGKGKKGGMILQQDSAIAIITDNNDVTYKVPSCIQGKLIEVNERLLKDVSLLGKEGDGYIAVVLPKPEQCEDIKASLMTEDQYLASLNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYL1</accession>
    <name>T1GYL1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYL1"/>
    <dbReference type="STRING" id="36166.T1GYL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008943-RA">
        <property type="protein sequence ID" value="MESCA008943-PA"/>
        <property type="gene ID" value="MESCA008943"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_2_1_1"/>
    <dbReference type="OMA" id="WDENWKG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="90"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008943-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="25409" checksum="65156BCAE454E93C" modified="2013-10-16" version="1">MPVELYYELGSPPCAAVYMTAKALELDIDLKEMDLLAGDQLKPEFLAINPQHCVPTIVDDELTIWESRPIMIYLFEKYAKDDKKYLYPEDPEKRALINQRMFFDVGTFYQKIIDYYFLPVFIKAEQKETDFHRLVEIVSFINFFLAGEKFVAGNTLTLADISLYATTMCLKSCGFDISKYSNVEQWFNHVDSIIPGAEENRKAGEIFKQFLANGNNDITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYL8</accession>
    <name>T1GYL8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032391">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYL8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008951-RA">
        <property type="protein sequence ID" value="MESCA008951-PA"/>
        <property type="gene ID" value="MESCA008951"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874315_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008951-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7026" checksum="358A87BB0AF87E98" modified="2013-10-16" version="1">RKRGKATKHHNHQHSAAKGGGFGNTGGNIESGNSISSGEFANLDKRKETYMENETDMKAQRSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYQ8</accession>
    <name>T1GYQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fumarate lyase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYQ8"/>
    <dbReference type="STRING" id="36166.T1GYQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008995-RA">
        <property type="protein sequence ID" value="MESCA008995-PA"/>
        <property type="gene ID" value="MESCA008995"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1528919_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008995-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="19437" checksum="175C0B6CB1D9A0C4" modified="2013-10-16" version="1">DLDKAVTALAGFEKYYAVTGQTYSRKVDVEVLGAIASLGTTIHKMCSDLRILANRKEIEEPFESTQIGSSAMAYKRNPMRSERCCALARHMITLFSNAANTHAVQWLERTLDDSANRRLSLSEAFLSADAALLTLLNITQGLVVYPKVIEKHIAQELPFMATENIIMAMVQAGGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYR4</accession>
    <name>T1GYR4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYR4"/>
    <dbReference type="STRING" id="36166.T1GYR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009001-RA">
        <property type="protein sequence ID" value="MESCA009001-PA"/>
        <property type="gene ID" value="MESCA009001"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2365572_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009001-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11646" checksum="06F996F27F0ADABD" modified="2013-10-16" version="1">SFIFEKQNSPTDSIDTIYYCNWGPYGLQVKKVRDYSTGQLNWVRENYVFQRNKIRKFSAHQVLRLREGYKYQQQTLNKVLENLPSFYFENCKSRCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYR5</accession>
    <name>T1GYR5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYR5"/>
    <dbReference type="STRING" id="36166.T1GYR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009002-RA">
        <property type="protein sequence ID" value="MESCA009002-PA"/>
        <property type="gene ID" value="MESCA009002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064123_0_0_1"/>
    <dbReference type="OMA" id="AIFHTEK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13431" checksum="391B28EA970DF5C4" modified="2013-10-16" version="1">MATHSGLTRWQEFRSNNAEDLLTGEDFGSKHNKGPEEIWYQRAVEQHLSKEDSFVFSVPFDAVEKSSEIIVTASQAIFHTEKRFKAPAAVVGFQFKHAALVSIFKNITSSVNILNYIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ50</accession>
    <name>T1GZ50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009144-RA">
        <property type="protein sequence ID" value="MESCA009144-PA"/>
        <property type="gene ID" value="MESCA009144"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1808404_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="17364" checksum="4F1A35E5D021A4C6" modified="2013-10-16" version="1">MNRRSTKIPHEELVSSGLEALLNNSVGVTRNMKKLLPRKIYSIRNTRATKEVFQNKRREVVTLARQKKRRFEKAQFEMLKFMEDRNQQNSSKTEENELFLFKATDEYKNKRWKSPKLYRESHETRKCHPMQEWLHTINIILQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZH5</accession>
    <name>T1GZH5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZH5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009276-RA">
        <property type="protein sequence ID" value="MESCA009276-PA"/>
        <property type="gene ID" value="MESCA009276"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431364_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039884">
        <property type="entry name" value="R3HC1/R3HCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21678:SF0">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21678">
        <property type="entry name" value="GROWTH INHIBITION AND DIFFERENTIATION RELATED PROTEIN 88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009276-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21285" checksum="EDE42AC78F1065C1" modified="2013-10-16" version="1">IRRERRPDRAVYVPRARRSLTTPPQVTNTLDPLITAPTTNGDISLKPRKVKSEKVSTKSQYSDENVNLVNMSTKTNMSTTPKAEVLRIDGGATNNIKDNDSDLVELKRASNEINRSNRRIIKQTFVSDVLEIPDIQSSDSPNDSVSCDPETDDWDTMFDDNGECLDPKIIEELSASVGKVKIETPKMDYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZJ2</accession>
    <name>T1GZJ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009295-RA">
        <property type="protein sequence ID" value="MESCA009295-PA"/>
        <property type="gene ID" value="MESCA009295"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906674_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009295-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7235" checksum="0660DD61002F47BA" modified="2013-10-16" version="1">MVVSRVYIQRNTRVNKEVYQAKGGKAPNFELNRVNLHQSMGKTKKTEGIKNRKNLRPIHMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZR2</accession>
    <name>T1GZR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009366-RA">
        <property type="protein sequence ID" value="MESCA009366-PA"/>
        <property type="gene ID" value="MESCA009366"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399489_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009366-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22440" checksum="C87FB7BD41B9A77F" modified="2013-10-16" version="1">RSGIDLFDAFQQILVKIICPIPQKGDKKGCNYQGISLFNIGYKILISILCETLKPRVIKIVGSYQCGLMRLCDFIAYSDNIDLIGRSSVNMVEFFLRLERAAGKRRQNKIHDFIPKHTAKELIMTMDSYNIDVVKNFVYLGSGVTSNNNFLFHFRKKNPFVNLRSYLRKRGVENKVQPRAKTTVPKSRRCPKDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZX5</accession>
    <name>T1GZX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication termination factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Replication termination factor 2 domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the rtf2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZX5"/>
    <dbReference type="STRING" id="36166.T1GZX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009436-RA">
        <property type="protein sequence ID" value="MESCA009436-PA"/>
        <property type="gene ID" value="MESCA009436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048955_1_1_1"/>
    <dbReference type="OMA" id="EFRWLHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16653">
        <property type="entry name" value="RING-like_Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006735">
        <property type="entry name" value="Rtf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027799">
        <property type="entry name" value="Rtf2_RING-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775">
        <property type="entry name" value="PROTEIN C20ORF43 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775:SF0">
        <property type="entry name" value="REPLICATION TERMINATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="309" mass="35629" checksum="7891222E3D5F8CD6" modified="2013-10-16" version="1">MNRADNTGVQLFRRHFKFFDEARINFRIHVYIVKFKDKDAERQFRWLHCALTQERLQKPIVMCGLGRLYSKQNIIEQLLEKDKMPESAKHIKSLKDIKDLKLTANPAFKDEDKCEGFLDTRHAPFICKLIGLEMSGKFRFIALWSCGCVYSERAFKETKSNTCAICQEPYETDDIVILNGTDEEIEEMRVKMEIRVAKRKTKKSKKEKEIKKEVEAVPVSSSSSVPSTSGAPSTSTSKPSTSKKLVATKRLGAAKDIELQDPEIKKLKKDFSVSKDPKATEVFKSIFTSHKSEQEQDRAHWVTYNPFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0A7</accession>
    <name>T1H0A7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0A7"/>
    <dbReference type="STRING" id="36166.T1H0A7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009580-RA">
        <property type="protein sequence ID" value="MESCA009580-PA"/>
        <property type="gene ID" value="MESCA009580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001357_12_0_1"/>
    <dbReference type="OMA" id="VENRHFH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09327">
        <property type="entry name" value="LIM1_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000055">
        <property type="entry name" value="Actin binding LIM protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000075">
        <property type="entry name" value="Actin-binding lim protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000098">
        <property type="entry name" value="Uncoordinated 115a, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000099">
        <property type="entry name" value="Uncoordinated 115a, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF9">
        <property type="entry name" value="UNCOORDINATED 115A, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="64"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="157"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="301" mass="33118" checksum="669B8C2F88B92113" modified="2013-10-16" version="1">KNKIYCAKCQKKCSGEVLRVGDKHFHKSCFQCSQCKKSLATGGFFTKDGVYYCIPDYQKLFGTKCAACNQYVEGEVVSTMGKTYHQKCFTCSECKNPFQSGNKVTNTGKEILCEDCLGKNSGSRKQQNQKPENPTKATTLLTSENKDKSKNSNYDPNDCAGCGGQLKEGQALIALDRQWHVNCFRCRLCNVILNAEYMGKDSVPYCEKCYQKSFGVKCAYCNRFISGKVLQAGENHHFHPTCARCTKCGDAFGDGEEMYLQGSAIWHPKCGPGPSEAGLILNGVAGSDTECDHLSTSGISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1H1</accession>
    <name>T1H1H1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1H1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010032-RA">
        <property type="protein sequence ID" value="MESCA010032-PA"/>
        <property type="gene ID" value="MESCA010032"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3413311_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010032-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="28" mass="3233" checksum="3CDAD812D7D74D6F" modified="2013-10-16" version="1">MHIGILKIMLIKSVSIKSEFRSLCSWVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Q7</accession>
    <name>T1H1Q7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Q7"/>
    <dbReference type="STRING" id="36166.T1H1Q7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010124-RA">
        <property type="protein sequence ID" value="MESCA010124-PA"/>
        <property type="gene ID" value="MESCA010124"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1442606_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="26"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="21728" checksum="5FE739C4CC4F5F65" modified="2013-10-16" version="1">MNNDQYYLEKVLISPGDIVEAGTSYKAKYMFSDSMFGAGVDEYLEKRLTGKSASTFVYVYDFASNNSVTKYFDHDDFYYGVAHCDDIEIFFPGFLWDQEIPSENLDIMRESLGKMWINFATYGDPTPGNAEIKWESTNKYPWNHARLGTLESNKLVVLKNENGYARSRYELWKNLKPHIGCISYGIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2E9</accession>
    <name>T1H2E9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2E9"/>
    <dbReference type="STRING" id="36166.T1H2E9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010394-RA">
        <property type="protein sequence ID" value="MESCA010394-PA"/>
        <property type="gene ID" value="MESCA010394"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362100_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010394-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10562" checksum="736780A45C59222D" modified="2013-10-16" version="1">MKGTQFDMNGSSRHRNHRNGGGHNNIHNHPHSLMTNFMSPMIGLSMMDSFFTDMPSNNGFSSFSSFNISSNGTNAAVKRTSTSTTFANGKKIMTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H361</accession>
    <name>T1H361_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium/solute symporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H361"/>
    <dbReference type="STRING" id="36166.T1H361"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010677-RA">
        <property type="protein sequence ID" value="MESCA010677-PA"/>
        <property type="gene ID" value="MESCA010677"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1017699_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF5">
        <property type="entry name" value="FI02094P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010677-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="29798" checksum="3B0BDC06ABD11552" modified="2013-10-16" version="1">MLIGASIYYVQINCGNQAMMQRFLSVPTVKDAKHCLIFYSCGIICIYLMTLYNGLLIFAKYHDCDPLTTGLSKASDQLVPLLVMDVLGGVFPGLAGCFVAGVFSAALSSLSTGLNSMAAVILEDFVKPNRSVPLTEKQTALVCKSVVIIVGILCIGLVFVVEKLGMIYFCCDSIKLNCLLFQYFERRFNSSLRLFGSVMYVVGEAVWFPIALYVPALTFNQVTGIGVHTITPIVCGIVTFYTCVGGLKAVVWTDVVQSGLMYGSILLVMIKGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3H0</accession>
    <name>T1H3H0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipid droplet-associated hydrolase</fullName>
            <ecNumber evidence="7">3.1.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lipid droplet-associated serine hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a cholesterol ester + H2O = cholesterol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36403"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17002"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:36404"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.13" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02186371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3H0"/>
    <dbReference type="STRING" id="36166.T1H3H0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010797-RA">
        <property type="protein sequence ID" value="MESCA010797-PA"/>
        <property type="gene ID" value="MESCA010797"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018394_2_1_1"/>
    <dbReference type="OMA" id="IGCWMIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010797-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="212" mass="24399" checksum="463DBE773EE3BE8D" modified="2013-10-16" version="1">HAGHDDPSAAALRQVPVLRGNERLFDLNAQIAHKVDFIEHYIPSHVKIHLIGHSIGCWMILQMLKERSDIKNQFESATFCSQQLKEWWNLQMFLMISAYFWLASISNVFLGVTLSYIRPTVMEKVVFLAKQEMDTVVNLDLDIVKENKNLITLYYGTTDGWVPVKYYEELKKQIPDVDAILDTHGIAHVFVLKSSEEMAQIVGDLVRKNRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H416</accession>
    <name>T1H416_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposable element P transposase-like RNase H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H416"/>
    <dbReference type="STRING" id="36166.T1H416"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011014-RA">
        <property type="protein sequence ID" value="MESCA011014-PA"/>
        <property type="gene ID" value="MESCA011014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1671320_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposable element P transposase-like RNase H" evidence="1">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="18539" checksum="7571A7772985E53F" modified="2013-10-16" version="1">MFDLRKKFPFDPLQSLRANIRRDECDKDLRVPQRKRRSLETIKQRPSRKAHGLFSNWHQPIFFDFDTQLRKGTLINLIKRLHSVGFEVRAMTCDLGSGNQALFNELGVSIERSFFLNPVDKEPVYVFSDAPHDLKLIRNHLIDDGFIVEKRDEANKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H482</accession>
    <name>T1H482_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BING4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H482"/>
    <dbReference type="STRING" id="36166.T1H482"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011086-RA">
        <property type="protein sequence ID" value="MESCA011086-PA"/>
        <property type="gene ID" value="MESCA011086"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1046927_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="6">
        <location>
            <begin position="169"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011086-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="266" mass="29540" checksum="12D5F002829C28FD" modified="2013-10-16" version="1">MHCVKRLFDVNLMEFLPYHFLLASASRQGYLSWLDVSIGELVGNFNSKLGDIRIMRQNPANGVLCVGGGKGVVSLWSPKVREPLAKLLCHSTPLSALAVNFNGQQLVTSGLENTVKVWDLRELSGPLAIYKFQKPVNHIDISQKGSMAFSFEKSCHIYDKIIYDDQPQIYLRHKADQHIQGLRFCPHEDVLGISSNGGFDSVLVPGSGYANFDAFEFNPYQTKSQRHRTAGGVGFGGAAGGDGDVGAAVGEKYRQYGFKWFKMYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4M1</accession>
    <name>T1H4M1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase VIIa family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4M1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011234-RA">
        <property type="protein sequence ID" value="MESCA011234-PA"/>
        <property type="gene ID" value="MESCA011234"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226191_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002082">
        <property type="term" value="P:regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.91.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036539">
        <property type="entry name" value="Cyt_c_oxidase_su7a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003177">
        <property type="entry name" value="Cytc_oxidase_su7a_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81419">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011234-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12210" checksum="7C86C844561D0D27" modified="2013-10-16" version="1">MLYLKFLQSLIQQSYAAGTGPKMAKKESKPSNNSRLKKMKAIQEKFQQKDGKPIFLKMGFRDQLLYRSTVLLALVGLFESCRTIYTMIYPAKPEEPQEEQEVSAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5K1</accession>
    <name>T1H5K1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5K1"/>
    <dbReference type="STRING" id="36166.T1H5K1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011581-RA">
        <property type="protein sequence ID" value="MESCA011581-PA"/>
        <property type="gene ID" value="MESCA011581"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018687_3_0_1"/>
    <dbReference type="OMA" id="YLLRNDY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000038">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16683" checksum="8C020035811A553F" modified="2013-10-16" version="1">MSAIGSYYRINNLVPASDGYFPPQEALDINEKQNSEAEVISQLNYIRDNILKKEDNELYQFLIKMEIPLQIFGIRWLRLLFGREFPLMDLLVLWDAIFADGDKFELPNYILVAMLIRIREYLLRNDYTTCLTYLMKYPSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5N7</accession>
    <name>T1H5N7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EnsemblMetazoa" id="MESCA012020-RA">
        <property type="protein sequence ID" value="MESCA012020-PA"/>
        <property type="gene ID" value="MESCA012020"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811020_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012020-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="16240" checksum="1EDA52223D68A627" modified="2013-10-16" version="1">DEIGWTRINEEVDIVIPKVLNHFETLGITHLDLPEIRETLSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFDYDFLLNILGIEGYGKVSGSLEDTVVYSELSVNVEDTRLEMDNFRIVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H641</accession>
    <name>T1H641_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H641"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012174-RA">
        <property type="protein sequence ID" value="MESCA012174-PA"/>
        <property type="gene ID" value="MESCA012174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1215990_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="26133" checksum="C7BBEDE17758DBB4" modified="2013-10-16" version="1">MRECRTPKDWSLLALQNTRTGKSHYLVICKCPEHYKMEGPMAHDQPTYASVPGIRVFGMMCVKPGYPIRKPSYQPPKRYQLQKPLYHASTSNKYSNKDPSLVVSRPTYNRVPSTNYGGIIPNDTFQNQHQANYQYLNRPDHTTITNSNHHHSDSNSFNSFGGGSYGRTKGQTEIDAEGRGNNSNRTNDITPQTTIASVIREKRSVSPFDDPPFPWDRLYEFEKTINWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6S6</accession>
    <name>T1H6S6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="T1H6S6"/>
    <dbReference type="STRING" id="36166.T1H6S6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012409-RA">
        <property type="protein sequence ID" value="MESCA012409-PA"/>
        <property type="gene ID" value="MESCA012409"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036024_0_0_1"/>
    <dbReference type="OMA" id="WTCDAND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048776">
        <property type="entry name" value="HAT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21183">
        <property type="entry name" value="HAT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Histone acetyltransferase type B catalytic subunit C-terminal" evidence="13">
        <location>
            <begin position="246"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="10">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="10">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="208"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012409-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="371" mass="44054" checksum="7AA750577B2D4352" modified="2013-10-16" version="1">RSVEDLEDGSQKFPPEMAHQIFGENENIFGYEDLAIDLYYTAGPLNIYLDINYGKKADDILKTLMKADDILSSLVDSLPNGICYLTNKAEFVKSLEEAEKFQPFGEVACVHFLDEGVERHFEIYYYNPKFVNFFSRLETFVLWFVDAASYIDLDDPQWNYFLCYEKFKNKKGELQYATVGYSTVYKYYAYPENIRPRISQMLVLPPFQKLGIGTNFIRKMYDHYVGLKNVIDITVEDPSDEFKKMRNYIDSKLCRDLKSFSESNLKKGFSKEMVKEARNELKINPKQSRIVYEILRLFYTNVHDESDYKNYRLSVKQRLNHHYYKQLRCIEKMEKLKIDTSRPLPTPEQRLEQLDEEYKQVEENYELIVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Z2</accession>
    <name>T1H6Z2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="5 12">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Cytosolic glucose-6-phosphate dehydrogenase that catalyzes the first and rate-limiting step of the oxidative branch within the pentose phosphate pathway/shunt, an alternative route to glycolysis for the dissimilation of carbohydrates and a major source of reducing power and metabolic intermediates for fatty acid and nucleic acid biosynthetic processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:15842"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5 12"/>
    <dbReference type="AlphaFoldDB" id="T1H6Z2"/>
    <dbReference type="STRING" id="36166.T1H6Z2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012474-RA">
        <property type="protein sequence ID" value="MESCA012474-PA"/>
        <property type="gene ID" value="MESCA012474"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013524_0_1_1"/>
    <dbReference type="OMA" id="YFRINIE"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 12" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="8 12" id="KW-0521">NADP</keyword>
    <keyword evidence="9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="13">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="47"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012474-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="36801" checksum="2B1E7168EA106F5A" modified="2013-10-16" version="1">TRVIIEKPFGRDSATSQLSDHLSKLFTENQLYRIDHYLGKEMVQNLMTLRFGNGIFSHLWNRSAIANVQITFKENIGTYGRMGYFDDFGIIRDVMQNHLLQIVALVAMPVLFHAHDIRDEKVRVLRAIPPLKIEDCVIGQYVGDKLHKKKSGLDDEKVPADSITPTYALTVLKINNEQWEGVPFIMRAGKGLNQGKAEIRVQFKDVAGDIFDGNPKRNELVMRIQPGEAIYMKINMKNPGSSGFEFHETEMDLTYENRYTEHYIPDAYERLILDVFQGNQMNFVRADELSEAWRIFTPVLHQMEREKVEPLKYVFGSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Z5</accession>
    <name>T1H6Z5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6Z5"/>
    <dbReference type="STRING" id="36166.T1H6Z5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012477-RA">
        <property type="protein sequence ID" value="MESCA012477-PA"/>
        <property type="gene ID" value="MESCA012477"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105263_0_0_1"/>
    <dbReference type="OMA" id="NEDGAMQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12249">
        <property type="entry name" value="RRM1_hnRNPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000024">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein q isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041337">
        <property type="entry name" value="hnRNP_Q_AcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18360">
        <property type="entry name" value="hnRNP_Q_AcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="129"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012477-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="233" mass="25932" checksum="73B51AC8893F4CB7" modified="2013-10-16" version="1">VAGKLDDIYKTGKLQHVELDDRALDALKEFPVEGALNVLSQFLESNLEHVSNKSAYLCGVMKTYRQKSKASQQGVPIPTTTVQVKGPDEDKIKKILDRTGYTLDVTTGQRKYGGPPPNWCDLPIPGNGCEVFCGKIPKDMFEDELIPLFEKCGEIWDLRLMMDPMTGTNRGYAVTFTTREAACNAVKQLDNHEIKPGKCLKINISVPNLRLFVGNIPKSKGKEEILDEFGKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7F7</accession>
    <name>T1H7F7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23052"/>
            <dbReference type="Rhea" id="RHEA-COMP:13689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="T1H7F7"/>
    <dbReference type="STRING" id="36166.T1H7F7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012694-RA">
        <property type="protein sequence ID" value="MESCA012694-PA"/>
        <property type="gene ID" value="MESCA012694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_2_0_1"/>
    <dbReference type="OMA" id="TEPTHEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF3">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="241" mass="27849" checksum="075EDCCC3B04ACD4" modified="2013-10-16" version="1">QNACEKKSFIFLRKELPVRLANIMKEIALLPKNLIGTNSVNQVNDWYLKSFEEVLEFEKTEPTHENLLVHIRNRHSDVVQTMAQGVLELKESQGGYVEPSLETSIQYFLDRLYMSRISIRMLINQHTILFGGNEESKGRHIGCLDPACDISSVVQDAYENARFLCDQYYLASPELVVREYNNLDDTLPVRTVYVPSHLYHMLFELFKNSMRAVMEQHDNATDNLPPIEVLIVRGKEDICVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7H9</accession>
    <name>T1H7H9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adipocyte plasma membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7H9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012716-RA">
        <property type="protein sequence ID" value="MESCA012716-PA"/>
        <property type="gene ID" value="MESCA012716"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_887313_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF88">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN HEMOMUCIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012716-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34815" checksum="D326FEFF7E788A0F" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGRPLGMAFDTLGNNLIVADAYFGIWSVELSQGKKKQLISPHQVLDKSPKAKIFNSVAVDKNGDIYWTDSLLKMDFTIFANPSGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRVDSGIILGSLHGFDNSARVVFLVLEVGKNLYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAW7</accession>
    <name>T1GAW7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02064602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GAW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000383-RA">
        <property type="protein sequence ID" value="MESCA000383-PA"/>
        <property type="gene ID" value="MESCA000383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1227465_0_0_1"/>
    <dbReference type="OMA" id="WPIFEES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="26385" checksum="62A87CB2E9FD7E90" modified="2013-10-16" version="1">PLEESLFEETQDWPIFEESFREFTAAFKYSDRENNFRLVDALKGCAKESVEGLLMHPNGVSEFGRLEIIVQWQXFTDKSLFEETQDWPIFEESFREFRAGFMYSGRENNFRLVDALKGCTKESVEGLLMHSKDDSDIMKKLKFEFGRPEIIVQWQLEKITNLPDLEENNMGQIMTFAIKISNMATFLRSCGNKQHLGNPILFEELVLKLPNSKRYGWAKNAFNLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB37</accession>
    <name>T1GB37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02198644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB37"/>
    <dbReference type="STRING" id="36166.T1GB37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000458-RA">
        <property type="protein sequence ID" value="MESCA000458-PA"/>
        <property type="gene ID" value="MESCA000458"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1490419_0_0_1"/>
    <dbReference type="OMA" id="YAYENCP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF349">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE BRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000458-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19571" checksum="E9F7A49E84F9FAD1" modified="2013-10-16" version="1">MMGFRYAYENCPQTDYFILVDDDYYVSTLNTLKALPQDEKSIFYGGTVHINAPVSRNPFTKWYQSTKDYPYSIYPDFVSGGFIMLTKNTLHKLYLGSQFTRHFIFDDVYIGIIAYKMGIQPINNKDCSNAKVSYDGPESYRTLVATHGYDDWTEMIKVWRECNDLGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB55</accession>
    <name>T1GB55_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000476-RA">
        <property type="protein sequence ID" value="MESCA000476-PA"/>
        <property type="gene ID" value="MESCA000476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592503_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9009" checksum="F938BCCABF31DA97" modified="2013-10-16" version="1">MGIQSILNFNPLDIKVKKLAALLAIRLENFGFYKRSIYGNTSILNESHFGIFFTVVRKLGTPIALASRVRSLPFDIHSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB65</accession>
    <name>T1GB65_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP synthase subunit beta</fullName>
            <ecNumber evidence="12">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="12"/>
    <dbReference type="EMBL" id="CAQQ02114271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB65"/>
    <dbReference type="STRING" id="36166.T1GB65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000487-RA">
        <property type="protein sequence ID" value="MESCA000487-PA"/>
        <property type="gene ID" value="MESCA000487"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_0_2_1"/>
    <dbReference type="OMA" id="VRCIMLA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000004">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="176"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="490" mass="52311" checksum="7C94A59553614351" modified="2013-10-16" version="1">MFSAVRAASKADKVLSFANQFNRSYAAKAAGKAAAASQGKIVAVIGAVVDVQFDGELPSILNALEVSNRSPRLVLEVAQHLGESTVRTIAMDGTEGLVRGQQVNDTGLPISIPVGVETLGRIMNVIGEPIDERGPIPSQKTAPIHAEAPEFVEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYNEMIEGGVISLKDKTSKVALVYGQMNEPPGARAQGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGSMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDANIIGQEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVARARKIQRFLSQPFQVAEVFTGHAGKLVPLEQTIKGFKQILGGEYDHLPEVAFYMVGPIEEVVEKADRLAKEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB89</accession>
    <name>T1GB89_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000512-RA">
        <property type="protein sequence ID" value="MESCA000512-PA"/>
        <property type="gene ID" value="MESCA000512"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075833_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13237" checksum="10083F246EB2C39D" modified="2013-10-16" version="1">MAWSFLPPPYYMPMYQYPAPVPTTAYYTATQPPMSKALMAAPLAPASPPEQPTRNMLNINVLIKNTKIVAQVDKQKMATTINCSNGRKWERDRDCVYRNDTTPKGRAGSTSSQDDCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBA5</accession>
    <name>T1GBA5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000532-RA">
        <property type="protein sequence ID" value="MESCA000532-PA"/>
        <property type="gene ID" value="MESCA000532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029355_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14078" checksum="76E090EA99B916B4" modified="2013-10-16" version="1">MNQQSKQQQFYQLNSTYINSSDCQLDFRENIEFKMKLFGIIILIATLVSMTLSLECYSCWDKESCAQRTHTPCLPPHRACFSLDTEMYGNRIIQKGCAIDTTFCNNYPEGCSHCTTDKCNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBN6</accession>
    <name>T1GBN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small lysine-rich protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBN6"/>
    <dbReference type="STRING" id="36166.T1GBN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000672-RA">
        <property type="protein sequence ID" value="MESCA000672-PA"/>
        <property type="gene ID" value="MESCA000672"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173519_1_0_1"/>
    <dbReference type="OMA" id="ACHNVQE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037760">
        <property type="entry name" value="SMKR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37932">
        <property type="entry name" value="SMALL LYSINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37932:SF1">
        <property type="entry name" value="SMALL LYSINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000672-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="9603" checksum="EF32682222DF59DE" modified="2013-10-16" version="1">MATKGKKKRNSKDSSGNGSGSEVVETTSKKKGGKKKSANKGKSFQCDIFNEAAMDNAYYVCHNVQDVLKSRGFAWPETQKKKKKGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBU5</accession>
    <name>T1GBU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBU5"/>
    <dbReference type="STRING" id="36166.T1GBU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000734-RA">
        <property type="protein sequence ID" value="MESCA000734-PA"/>
        <property type="gene ID" value="MESCA000734"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117345_0_0_1"/>
    <dbReference type="OMA" id="INTPRCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24299">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24299:SF21">
        <property type="entry name" value="OS09G0441600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000734-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22964" checksum="B2A609518A8FA525" modified="2013-10-16" version="1">MGFIIVFLIVILIAISLYSYLRVFYECIRKVKIAKSNVKITGNSSRGTSIEITDSTTDDSSKVEVFAQAPGPFPWPVLGNLALLGKYDVPFQGFTELAKQFGDVYTLTLGTTRCLIVNNLDLIREVLNQNGKFFGGRPDFLRFHKLFGGDRNNCKKNITFYVKYPINTPRCYKPIYKPTRRLQSTSTDAEVFYKPSSDFFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC31</accession>
    <name>T1GC31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein SPT2 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC31"/>
    <dbReference type="STRING" id="36166.T1GC31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000827-RA">
        <property type="protein sequence ID" value="MESCA000827-PA"/>
        <property type="gene ID" value="MESCA000827"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019714_0_0_1"/>
    <dbReference type="OMA" id="GPMATPH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013256">
        <property type="entry name" value="Chromatin_SPT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054552">
        <property type="entry name" value="SPT2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691:SF8">
        <property type="entry name" value="PROTEIN SPT2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691">
        <property type="entry name" value="YEAST SPT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08243">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22878">
        <property type="entry name" value="SPT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00784">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPT2 homolog N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="145"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="145"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000827-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="504" mass="58781" checksum="01EF2CFA5CC37399" modified="2013-10-16" version="1">MDFGQLLHVAKQNNSSQKRKDDNNKFYSTKFAPPKKETKAEKRLSENIKKFLEKREIEERQKAKEEKEKRDKLIASRDDKSKNKIKKMLKVIKSANKSVLEDAKGTETAVTVNGSEQPDDDDYGYVSNEAAALFAKYCEKVKDVKDDKGFAKSRPQSRDDLKGTKDRVRQALMKEQEEKLHTGQRVRTSNISSTPQKSERSTSRGGRELYDPKAEKEAEERKKREEDAKKKANLKKRPPPPPMNFNDLLKLAEKKQFEPVATEVVEGKKEMEERERRKNEKLKPKAPGPSKSQVPSTSSKEKPKEDPKKVPSKPRPYDGPTRDFPPKPNGVPSTSSSSSQSQKKPLPKEVPQKTREFPPKDIQKTRPFPPKDIQRTRDFPPRDFPPSDVRRSKPSSSYDSRKPQKRRMIEDEDSEEYDSEMDDFIDDGEDEEDYSKHIRDIFKYDKSKYRDDYDDMPMESSFAQMQREEAFSKKMGLKEDLEDMKLEEEMKKKKEMMKKKMKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC87</accession>
    <name>T1GC87_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000891-RA">
        <property type="protein sequence ID" value="MESCA000891-PA"/>
        <property type="gene ID" value="MESCA000891"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888314_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000891-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7207" checksum="3A99A010BDDBB77C" modified="2013-10-16" version="1">MEARILQILANKFGDTLVKLPRLTRLSQNSAQSFQLLSMLKTISMLMIMQRSIKAKLIRSSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCG3</accession>
    <name>T1GCG3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000975-RA">
        <property type="protein sequence ID" value="MESCA000975-PA"/>
        <property type="gene ID" value="MESCA000975"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742963_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000975-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7809" checksum="50F13ADD61D2DE5F" modified="2013-10-16" version="1">MDRRSSGRFSLNGSQFNNLPVAESTQIQSSFLSSPRQSLGNCSYTSNSPSTNRGRIQKTIMSPASKIDPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCT3</accession>
    <name>T1GCT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCT3"/>
    <dbReference type="STRING" id="36166.T1GCT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001106-RA">
        <property type="protein sequence ID" value="MESCA001106-PA"/>
        <property type="gene ID" value="MESCA001106"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_967376_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF295">
        <property type="entry name" value="AZ2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001106-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="33948" checksum="1D3422EF40AA3D6B" modified="2013-10-16" version="1">MATLQSLATDLKLSHDDDTSDDEPSDEDIFSDNSNDKKLKDISNLSKEQYTPKKDKNDDIPEIVLVKTEIENYKNTFQNHRKILRISTKKPDSYKPMFAKLIRTNYKFVPDSMVVNILYSKPQSLEKDCCHKCKLIFNKTLDYLNHKYQNHMKSKTVLRNKYQICRQCGKTQRSKKALYNHQFVCFARLNKYYECSGCSQGFYDYKKMKIHRFSCLINFNTEKKERRFSCDLCEKKFSRKGGLTQHKNSFHFNIKPYVCTVCGHKYALKGDLSRCRHKRALAKGFVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD63</accession>
    <name>T1GD63_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001244-RA">
        <property type="protein sequence ID" value="MESCA001244-PA"/>
        <property type="gene ID" value="MESCA001244"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751803_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001244-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17472" checksum="0DBB068714CC24CF" modified="2013-10-16" version="1">MVWMLTKSNIYFELQTLVDSSTEQQSIIFSRRYFWSDLRTIICWLNSESLNYKEYESCGIDEILEVRNLEEMEEFRQGDALSCSFFNILLKMILRAAETDTKNIIINKSTQILGYFVVERTTGSQQLKEEAKSRGLRVKAEYCSQADVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEC8</accession>
    <name>T1GEC8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001689-RA">
        <property type="protein sequence ID" value="MESCA001689-PA"/>
        <property type="gene ID" value="MESCA001689"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852282_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001689-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7356" checksum="95F5822312490477" modified="2013-10-16" version="1">MSFYEGYVAVKGYVSGRFAPSKGAPSHTEPNSSEYVPILQYGIQRPKEHLLTWTAELEPTKVLLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEF7</accession>
    <name>T1GEF7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02017137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEF7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001719-RA">
        <property type="protein sequence ID" value="MESCA001719-PA"/>
        <property type="gene ID" value="MESCA001719"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925251_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001719-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7265" checksum="1170B7DDC4852E83" modified="2013-10-16" version="1">MYLSCRADLYYSCHHSKHEFGIFLARNFLATAPLTMVLPFPLLAFRPRFKVLQWILRNADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEH2</accession>
    <name>T1GEH2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEH2"/>
    <dbReference type="STRING" id="36166.T1GEH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001734-RA">
        <property type="protein sequence ID" value="MESCA001734-PA"/>
        <property type="gene ID" value="MESCA001734"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111645_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577272" description="Peptidase S1 domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="25"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001734-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="12754" checksum="8CE3DF20B4FF2B86" modified="2013-10-16" version="1">MKLVVVVLLIGIVADSLGAVPQSRIIDGHDARYAEAPYIVSLQRAGEKHFCAGSIIHKNWVLTAGHCLIYSSFYVVAGLTKRSRTWEFKLGKFPATDNFSSTKNMKETLLLMILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEY5</accession>
    <name>T1GEY5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001911-RA">
        <property type="protein sequence ID" value="MESCA001911-PA"/>
        <property type="gene ID" value="MESCA001911"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906696_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001911-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7364" checksum="AB654B9F75164C14" modified="2013-10-16" version="1">MKGICNCSRILFMYLLKQRRDPFQINYKRAQEIQTKCDAEPSMSTVCNLRVNYKTMGSNRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF79</accession>
    <name>T1GF79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF79"/>
    <dbReference type="STRING" id="36166.T1GF79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002010-RA">
        <property type="protein sequence ID" value="MESCA002010-PA"/>
        <property type="gene ID" value="MESCA002010"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2365578_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF153">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10671" checksum="4C478AC8EBFEAA3B" modified="2013-10-16" version="1">SLLRKTKIEKELIDYITYGTVIQEVKTSNIGREAALAAGFSDKTPAHTVTMACISSNVAMTTEWFCLHCLCNIVINYILAYADMILMSLGEYQTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFA1</accession>
    <name>T1GFA1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the transition zone in primary cilia. Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM237 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFA1"/>
    <dbReference type="STRING" id="36166.T1GFA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002037-RA">
        <property type="protein sequence ID" value="MESCA002037-PA"/>
        <property type="gene ID" value="MESCA002037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_673178_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029409">
        <property type="entry name" value="TMEM237"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15383">
        <property type="entry name" value="TMEM237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="409" mass="46668" checksum="6232A3B333DD3A90" modified="2013-10-16" version="1">MDKKDNTPKVHKKKRSRSRKSRGQTDAVQIDNATEKTEDSSNKLSNHPAVLINGYEQDEEDFLDDIEKRSSISLSPRHQGDSISVGGGDNNVNNDMPRRPSTNSTSRLEVKSSFKNQSNRGLTSFMDRELGLSKKSSSFSDPQASPAQRSMRSRSSLDKSPRRKRSKSESKRRKERKLIAAGEMEVKQANETLMRYLKQCSEMNDASLSGELEIDQNYDERKVHRKTKAERDKKGQIINILPTDADEIYNPFTPVVSPTDEKPTCINKMFVQTSTGYRPVDHCFYKNSVNGDIESNRMGLNRSSNMSCIQRICLIFSTLCHGLLGGLAFAHLLFILSTKPKDWSLNLTEHYSSFANTYSIAFYVLSIICLISVIFRSFETPNYFTVLRKNQIFANEMYKTLRENWNSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFQ7</accession>
    <name>T1GFQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002204-RA">
        <property type="protein sequence ID" value="MESCA002204-PA"/>
        <property type="gene ID" value="MESCA002204"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226204_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588257" evidence="2">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002204-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="11828" checksum="50422D62827E80B2" modified="2013-10-16" version="1">MKINGEFRILLYFVAIFLLSTQLGLVNGDGKCFKEDRFVAILSAFWIKLFGPVIIGLATGDIASFSKGFSASLAFDNIEVKNLKREFIIPDITITPEIIEHKCTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG61</accession>
    <name>T1GG61_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002370-RA">
        <property type="protein sequence ID" value="MESCA002370-PA"/>
        <property type="gene ID" value="MESCA002370"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1919441_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002370-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="14992" checksum="5F754FBE1703940E" modified="2013-10-16" version="1">MSTCNKITSCTWLDLRTTCLIIGAIDISINGLLRLFISLLQFGILIKLIYVLYIIVGVLLIFAILKEKTQFLVYVIILQGFIVVLYLILFISGYVDRGIYNFLIISWDIILGIFVILIVNAYRKKVNGTLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH02</accession>
    <name>T1GH02_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069697">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002684-RA">
        <property type="protein sequence ID" value="MESCA002684-PA"/>
        <property type="gene ID" value="MESCA002684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925237_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015019">
        <property type="entry name" value="LAMTOR3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08923">
        <property type="entry name" value="MAPKK1_Int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6767" checksum="590266B202B833CB" modified="2013-10-16" version="1">MSDAKKFFKNIINNRVSGLYLILVTDLDGVQLLRVTPDDKSTDVASSFISSFVLSSDQLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH10</accession>
    <name>T1GH10_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041031">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH10"/>
    <dbReference type="STRING" id="36166.T1GH10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002692-RA">
        <property type="protein sequence ID" value="MESCA002692-PA"/>
        <property type="gene ID" value="MESCA002692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877791_0_0_1"/>
    <dbReference type="OMA" id="ICIGEPS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF29">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15147" checksum="A46A823AFA9DFB6D" modified="2013-10-16" version="1">MTLKDKWSSILPYADDIDIIWRTPSEIIDKFLAIEKGANYVGLNVNGSKTKYMVSSRNQQRHNDLGSNITMGHHNIKIVKNFMYLSSEVTSDNNISAEIRTRIILGSICIGEPSETVAIQTSIVQKKSSASSTIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHC0</accession>
    <name>T1GHC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHC0"/>
    <dbReference type="STRING" id="36166.T1GHC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002813-RA">
        <property type="protein sequence ID" value="MESCA002813-PA"/>
        <property type="gene ID" value="MESCA002813"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130762_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF36">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 9, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="77"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002813-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="19837" checksum="BFAD87D795818BC8" modified="2013-10-16" version="1">MTAALRNSARMESEPARLLFLSSWIAIRTFSIFKFSLLILQFSSLLKNSTKNSLFSSFIISSLQNDFDEPHISTPVPEFIESIRNITIPAGRDAVLACLVRNLGKNKVGWLKSSDQTVLALEDKVVTHNSRITIVNEDPSTWKLKIKQVKESDRGCYMCQINTSPMKKQIGCVEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHE3</accession>
    <name>T1GHE3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033127">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002839-RA">
        <property type="protein sequence ID" value="MESCA002839-PA"/>
        <property type="gene ID" value="MESCA002839"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847400_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002839-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15799" checksum="6DFB95CE7347F9EC" modified="2013-10-16" version="1">MQNKVISDEIVPWNDCCDDVFYPKLILYLLPVVYNKCFMESDGDPTSPCFHTCIFKMMGSYGPNGLNSKVLKRLIGSNNMMGEESGWKKQNADKILDKCLSQIDTKSYIECNEDLKSFSFCYFAELFMACPDFNESNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHK2</accession>
    <name>T1GHK2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHK2"/>
    <dbReference type="STRING" id="36166.T1GHK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002906-RA">
        <property type="protein sequence ID" value="MESCA002906-PA"/>
        <property type="gene ID" value="MESCA002906"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850169_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002906-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15297" checksum="4977AC3AEE32A97B" modified="2013-10-16" version="1">LDDSDNDIITDFLCNQGEKDATKNNIYSHENISNSSSSSTKPIVPIISVTHTPASYKYGHILEDTLNHIEMIRESLVLMKKSTLCTNTNACDINDINNGKLSANSSTSLPDLTQNNLLTDRRKSCTEIDDLSYGGNKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI52</accession>
    <name>T1GI52_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02012038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003119-RA">
        <property type="protein sequence ID" value="MESCA003119-PA"/>
        <property type="gene ID" value="MESCA003119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485909_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10134" checksum="EAEC842BFDC0B5A4" modified="2013-10-16" version="1">MYFDNNTNQQKPDLKFRTVPNFKKQMDFNYKTFIYPSKSRLISTKLAASSVSEKRCNRFYLESTTFLSPSSSPSSFNIWNNSDGVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIE7</accession>
    <name>T1GIE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02389418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIE7"/>
    <dbReference type="STRING" id="36166.T1GIE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003223-RA">
        <property type="protein sequence ID" value="MESCA003223-PA"/>
        <property type="gene ID" value="MESCA003223"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1742629_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="82"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003223-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="17928" checksum="1033CCE684ED405A" modified="2013-10-16" version="1">MSAREAYARLHLLRCLKISIASEMEHMTPQMEHYKKTETNWRIPESEIDKLFHAETQEQQKRILEDFARDLPVTNRTIHGSVRFCEKCQIIKPDRGHHCSVCGMVNNCVNFTNYKFFVLFLGYALLYCLYIALTSLEFFIKFWKFMKNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJK2</accession>
    <name>T1GJK2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nipped-B protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJK2"/>
    <dbReference type="STRING" id="36166.T1GJK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003651-RA">
        <property type="protein sequence ID" value="MESCA003651-PA"/>
        <property type="gene ID" value="MESCA003651"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_657716_0_0_1"/>
    <dbReference type="OMA" id="ANGVMSY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003651-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="47867" checksum="CB8FCC90F0A755EF" modified="2013-10-16" version="1">MYEKIRSSILTDIFTSIDRLPCSKKNLRPFKLDNNGGSIQMVTALVLQLVQCSTILPESLSDTKKSKGGGNENNEDTANDSENKNISDKDIKILKKFDVAIGIGANFLTTFLSKCKTKSNETDFRPLFENFIYDLLSTVNKPEWPASELLLSLLGTLLVRCMSDKTKEISTRLASLDYLGIVAARLRKDTVESKCKVNVIDAMIASIKLEQEKEGDCKKKNNNIVLDPEEERTEFLQKILLDYLAVNAQEENLVWDYARHFYLVQWYRDIINQRKRIAEGEKGYASKKKKTHKKKRRNDSRTDEELTSSSEEEDEDEEAVKKSSKVIDVELNLEIFKILDNRKRYLLSKVKAFSDNNCTSQHIKTYIDYNNAHLIAQYLANGVMSYCVMLEQLTSQAGEQQQKNREVWKAAIHQAESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJL5</accession>
    <name>T1GJL5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02135865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02135866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02135867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003664-RA">
        <property type="protein sequence ID" value="MESCA003664-PA"/>
        <property type="gene ID" value="MESCA003664"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963439_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003664-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6735" checksum="CCBC231FA5523051" modified="2013-10-16" version="1">MEQLLSIIAHLITPQSEDNTEDVPPPTVSELFFLVTTDMYCPFKEPDGLRTCYKSELET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKB1</accession>
    <name>T1GKB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alcohol dehydrogenase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02125962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKB1"/>
    <dbReference type="STRING" id="36166.T1GKB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003931-RA">
        <property type="protein sequence ID" value="MESCA003931-PA"/>
        <property type="gene ID" value="MESCA003931"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_3_1"/>
    <dbReference type="OMA" id="VERCMNK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF12">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003931-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16121" checksum="0234CC504EC81941" modified="2013-10-16" version="1">MGCKAAGAKKIYGIDLNSSKFELAKQFGCTDFVNPKDHEVCIGNVHTMRAALESCHKGWGTSVIIGVAASGQEISTRPFQLVTGRTWKGTAFGGWKSVDDVPKLVQDYLNKKMLVDEFITHTMGLDKINEAFDLMHKGESVRSVVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKI1</accession>
    <name>T1GKI1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKI1"/>
    <dbReference type="STRING" id="36166.T1GKI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004005-RA">
        <property type="protein sequence ID" value="MESCA004005-PA"/>
        <property type="gene ID" value="MESCA004005"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_0_3_1"/>
    <dbReference type="OMA" id="SADTCYG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF136">
        <property type="entry name" value="GH06673P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="104"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004005-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="336" mass="37093" checksum="E3F08CD2306BECA3" modified="2013-10-16" version="1">MKNGRVSGVCTHISECSQNQFEATDSFIYTKCDSDDFLIICCPTKFSSVNSISDRFENTRISEQKCKEYSAAIYVNSVFLPLSVGAPSIITKKEQCAHKIQPLVVGGEEARLHEFPHMALLGYVESNNKVSWDCDFYISRSDLKIVRLGELNYESTEDGAEPIDFGISKTFVHPKYDNPPDSSYNDIGILKLSGKVKLTPGVRPACLPTSAELSERSIATGWGQTLFAGSPSSHLLKTVLEKFTKKECKAVFKAGSINLPNGIDDSQFCVGHRTEIKDTCNGDSGGPLQNYHSSLKCMYTLYGITSTGQGCGIIGVPGLYIQIWNYIDWIENIVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKR5</accession>
    <name>T1GKR5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein tipE</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKR5"/>
    <dbReference type="STRING" id="36166.T1GKR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004092-RA">
        <property type="protein sequence ID" value="MESCA004092-PA"/>
        <property type="gene ID" value="MESCA004092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050807_0_0_1"/>
    <dbReference type="OMA" id="QRFNFYA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017080">
        <property type="term" value="F:sodium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031578">
        <property type="entry name" value="TipE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12335:SF4">
        <property type="entry name" value="TIPE HOMOLOG 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12335">
        <property type="entry name" value="TIPE PROTEIN TEMPERATURE-INDUCED PARALYTIC E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16972">
        <property type="entry name" value="TipE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="34403" checksum="6D8FB2FBC894C074" modified="2013-10-16" version="1">MRSGSSELLLEQQQQELRLRKKRELAPKKKNDKRRFRSWRERVRFYTTSTLVLITVASGSSLLFLVPLYVDPALSTLAHNFIDKPTLCTTVRRENLIGLFNCSWSSCREGCTSDMYKCTNIYVTYIENISLPENLTDLSTLSPLSYNPDSTTSIALHINDYNNFSAVNNGNNEQSEESTLWVNIKGCGYPPTVTCQDFNDMYGVEGALFHNKTIVLTSYNHDEQIEVIIHYFFVPFVICFVTGVALCIMHCDCTCKTDTSYLRHRMPLRRPRIENLSDTSISTRVDLLTPAIEVFKAHPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKZ1</accession>
    <name>T1GKZ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02108091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKZ1"/>
    <dbReference type="STRING" id="36166.T1GKZ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004174-RA">
        <property type="protein sequence ID" value="MESCA004174-PA"/>
        <property type="gene ID" value="MESCA004174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023886_0_0_1"/>
    <dbReference type="OMA" id="NATINDQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000003">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000003">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056759">
        <property type="entry name" value="DYH2-5-8_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF4">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25007">
        <property type="entry name" value="DYH2-5-8_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein axonemal heavy chain 2/5/8 coiled-coil" evidence="15">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="14">
        <location>
            <begin position="175"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="14">
        <location>
            <begin position="356"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="712" mass="83885" checksum="EEF6A51A82E74C4D" modified="2013-10-16" version="1">MDYVYAVMNEMDRKLDRPIRDLDDVRMIMETLAKIREQEVDMELRIDPIEEAFNVLTRYEVPVEREQFDLVDNLRATFQNLQDCALKSQVKLLEMQPSFQDDLKDNLETFKQDKINYVTEYRTAGPMQPGLTPRDASDRLILFQNRFEGLWRRLQTYQSGEELFGLPQTDYPELGQIRKELNLLQKLYKLYNDVIDRVSSYYDIPWNEVDIEEINNELMEFQNRCRKLPKGLKEWPAFHALKKTIDDFNDMCPLLELMANKAMKPRHWQRIMDVTKYIFEFDSEGFSLKNILEAPLLKHKEDIEDICISAMKEKDIEAKLKQVTNEWSIHELQFMSFNNRGELLLRGDTTAETIGQKQIQQWVFDLSNSNEILERWLLVQNMWVYLEAVFVGGDIAKQLPKEAKRFSKIDKSWQKIMQRAHETPGVVACCVGDDLLKQLLPHLQEQLEICQKSLSGYLERKRMMFPRFFFVSDPALLEILGQASDSHTIQNHLLSIFDNIRHVKFHDIEYNKMMAIISSEGETIQLDRAIRAEGSVETWLTQLLMTAQASLHSIIRTANATINDQNFALLIFLEKMPAQVSLLGIQMIWTRDAELALMQARHERKIMAETNNKFLELLNTLIDQTTRNLTKVERTNFETLITIHVHQRDIFDILCRMNIKSANDFEWLKQCRFYFKEDLDKTWISITDVTFTYQNEYLGCTDRLVITPLTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM78</accession>
    <name>T1GM78_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GM78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004646-RA">
        <property type="protein sequence ID" value="MESCA004646-PA"/>
        <property type="gene ID" value="MESCA004646"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397562_0_0_1"/>
    <dbReference type="OMA" id="HIETQET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028085">
        <property type="entry name" value="FNIP_mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028084">
        <property type="entry name" value="FNIP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634">
        <property type="entry name" value="RE13835P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634:SF9">
        <property type="entry name" value="RE13835P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14637">
        <property type="entry name" value="FNIP_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14636">
        <property type="entry name" value="FNIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Folliculin-interacting protein N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Folliculin-interacting protein middle" evidence="3">
        <location>
            <begin position="237"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Folliculin-interacting protein middle" evidence="3">
        <location>
            <begin position="328"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004646-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="693" mass="77820" checksum="944C5CB6454D6123" modified="2013-10-16" version="1">MVFGALAMSFRGTALKVHWLQSPPSKLLCSQVFLSPSSPSTSIYSSCTGKSGGCANSLSPRHSIASDLDSDSLSMNSFSMPFSVHVKRNSTLAVDVPEMSLDSNSSHNYPDSGFQDPWTTITSASSYQYSTRSSIGSILSDNDSFRKNSLDSSYMSSTFSSKSSDGSLQRRISRNLWTSFENRNSVSDFIGFIGDNSQSSPTGGSEGCVTARYQRQHIETQETHSNPEIRCRRPSVHKQQIRREKLGLAICITMSESFQEEMEQYCCEHMALFESMLCRLRAQVEQAYLRRKDFFQDLDFNTCCQVLLYFAIDLTGVYEGCPKILDWDPYNALWGQLGDLYGAIGNPPKLSRTIIYGQEKMMIEKLLYVMTYFIRCSEVRRATKKEVFNKDEMDDLINQNSINNIKNEVFLCQKKSDPNKKDGSTGGLRKSKTCSNSLKHLNLTEEELNEVLDQFKIDEVISINKKNEIPNVLAFRDSRLVKQELRIGNYLMDTGIEKSAIVNGRLELGKLKRRKINKNSKSYALIKLLVTTPENVEMAVDSGDEAEEALENGTNETDSGNKENKLPKHNLLWSLMPETPSDGISLQNLSNIGRKDGNRPLKRMGKSQSFAELNGNREFQLEPENKQKTYISLSDLITANSMGNSQRMSWGIEPIKETALVNIKKSTESLVVEDGNTDTEDVKDTSLLAACXR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM86</accession>
    <name>T1GM86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein Lines N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM86"/>
    <dbReference type="STRING" id="36166.T1GM86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004654-RA">
        <property type="protein sequence ID" value="MESCA004654-PA"/>
        <property type="gene ID" value="MESCA004654"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_802378_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032794">
        <property type="entry name" value="LINES_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024875">
        <property type="entry name" value="Protein_Lines"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16057:SF1">
        <property type="entry name" value="PROTEIN LINES HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16057">
        <property type="entry name" value="WINS1, 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14694">
        <property type="entry name" value="LINES_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein Lines N-terminal" evidence="1">
        <location>
            <begin position="116"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004654-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="346" mass="39125" checksum="0055431F6F6EF5A4" modified="2013-10-16" version="1">METPTSLTGSKEQIVLKHIYTAAPHNDSWLKKLVDNLFSFERLDALAIQKMCFSQEIIRRIVEWRDTDLHPLEDEAEVGNAGIPIENNPFATGLQPPEEDTHQRNLSVETNGSCQIVTLTDAESFDTTQLKCQTIKILENKWPALVRNMKNLISGGHLNGSSTSAENCVLTFLQLWENILSVKTNLSIVDILPFYSQLDTFEELLHQNNFTSCTVYKQMLSLFNEALCYGSTLALQDSSPEQLCLLAQQVVRHVNYRLLEYLPRRQPENCVSLLGFKGTNRDYPPGADLMFPRIGNPGELDLEMDKTILQKMFLLVLKSVAVTVKEIRSDSSDSSIDSQDNDLIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM93</accession>
    <name>T1GM93_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02042034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM93"/>
    <dbReference type="STRING" id="36166.T1GM93"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004662-RA">
        <property type="protein sequence ID" value="MESCA004662-PA"/>
        <property type="gene ID" value="MESCA004662"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_834938_0_0_1"/>
    <dbReference type="OMA" id="CEKQKAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="333" mass="37862" checksum="3709B1D65D4078C6" modified="2013-10-16" version="1">MNLFPPRCYHESLENKSFHDIAQIYSRFENPSCTRLELLHGDNYTESPLKCDRWIYEYDFGYRSMNTDLNWVCDSAYKARVGQSLFFIGSVVGTLFYGCLSDKIGRLPALILSNLCGFLGDFSTIFTRTATTFSLCRFISGLAADTNFFLMYIIVLEYIRPSMRTLGINLAVGLFYTLGLVFTPWLAVVVGNWKYYLAFTSIPILSVMLFYFVIQESAQWLVTRDNIDGAILRLKRVAKFNGKNVSNDDFNEFRKYCEKQKANAPDGENFGRGEKMFDFPCLTKAADDDDEEEGNCNSKDVEIAQLSRVASKVDATSNSEDSSELNANETSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMA2</accession>
    <name>T1GMA2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMA2"/>
    <dbReference type="STRING" id="36166.T1GMA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004671-RA">
        <property type="protein sequence ID" value="MESCA004671-PA"/>
        <property type="gene ID" value="MESCA004671"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="OMA" id="VSECCPK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF7">
        <property type="entry name" value="RH09188P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004671-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="415" mass="46213" checksum="C93E917C1D628A5A" modified="2013-10-16" version="1">MDYIGRKKTLLITQVPACFGWILIACANDVGLIYAGRLFVGLGSGMVGAPARVYTSEVTQPHLRGMLSALASVSISFGVLFQYTLGSITTWKVLSIISACLPVLAFVLMLLMPETPNYLIGKDKQVKAEKSMTKLRDSKYNINTEITQLVDFNAKLHPPQDPNKLDEKPDKQQLIKALMSPSALKPFAILFIYFLMYQFSGVNTITFYAVDIFKASGTNLDKYMCTIILGVVRLVFTIVACISLRKLGRRPLTFISGIGCAATMIGLGVYMYYKQAWELAGEEPKVTWFPVACIFIFQVTCAVGFLVVPWVMIGELYPQKVRGIMGGLTTCSAHIFVFIVVKTYPFLCDVIYVHGAFMLYGCISLLGTVFFYFCLPETKGKSLQEIEDYFSGRSDSLKRKKNTNNEKVQEGKLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN92</accession>
    <name>T1GN92_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN92"/>
    <dbReference type="STRING" id="36166.T1GN92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005036-RA">
        <property type="protein sequence ID" value="MESCA005036-PA"/>
        <property type="gene ID" value="MESCA005036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755621_0_0_1"/>
    <dbReference type="OMA" id="AKEYHAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021773">
        <property type="entry name" value="TPC11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11817">
        <property type="entry name" value="Foie-gras_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577538" description="Trafficking protein particle complex subunit 11 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex subunit 11" evidence="2">
        <location>
            <begin position="173"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="367" mass="42442" checksum="FE453AF3909618C1" modified="2013-10-16" version="1">KHLHVLPAVVVLFLDLEWNDPQWPERQVHCANLLQELRNNLQDRNTRIALVLLQKNTPQEDLLASERAASLTNACGITNKMMFILPHNDHLMGYILRLESAFLEMAQTYYATMSKTIRSHREQLATPSHVTLRIRHQFKLGFVAEMRSDFSTALKHYSQAYATLDDIKITDQNCLEIKTVAGFLNYKICRLMFKLNIPRDSINQFMTHIEKYKEQIGYKDLIFEHYAWLSMQNSTFADLFCEAIKNGLPALQTQHPGIYYHKGSEYIVKRRNAFRVCCSLSPEIGPTTVPISTAFSTITTAQEMERAFNHSAVIIHLLGLAMNQYKQYRCMRFKKKITIEMAKEYHASGDHSKALTYRSIPDICEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNG8</accession>
    <name>T1GNG8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005119-RA">
        <property type="protein sequence ID" value="MESCA005119-PA"/>
        <property type="gene ID" value="MESCA005119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725084_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8526" checksum="373F9E4ED8F69979" modified="2013-10-16" version="1">MDIERKTSKEMNRKDEINLCHREMENFIGYYTRKGTESAEKRAVLGNRNQGNFLCNAAKNFPTRNEERVEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNV8</accession>
    <name>T1GNV8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02086542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNV8"/>
    <dbReference type="STRING" id="36166.T1GNV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005269-RA">
        <property type="protein sequence ID" value="MESCA005269-PA"/>
        <property type="gene ID" value="MESCA005269"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280531_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051286">
        <property type="entry name" value="JAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE HOPSCOTCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807:SF7">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE HOPSCOTCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="74"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005269-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="24724" checksum="DC2952B5EBBA824A" modified="2013-10-16" version="1">KEFDHISDLIKSIETDGDKFCIHPSEYDKPAMLLLCSPNKQLKTKKVVTDVNEADLKVKGGRIFDFHTDLQLYISSKTLLDNGKITEMPADWILSGEKQIKVSVKICNSEANVGDFLKLSNNWSKMYAPEFIKLYGVTLSMPLSMVMEYAPFGQLQQFLQKEKDSIPFCCLLEAVYSLVRAIIYLQENRIVHTEIRCSNLFVTRYEPKSNILVTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPM8</accession>
    <name>T1GPM8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02170362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPM8"/>
    <dbReference type="STRING" id="36166.T1GPM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005562-RA">
        <property type="protein sequence ID" value="MESCA005562-PA"/>
        <property type="gene ID" value="MESCA005562"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_67_2_1"/>
    <dbReference type="OMA" id="DCESEMS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05597">
        <property type="entry name" value="STKc_DMPK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000014">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001209">
        <property type="entry name" value="Serine/threonine-protein kinase MRCK beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF66">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GENGHIS KHAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="252"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005562-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="324" mass="37194" checksum="F6770EE0A83DB153" modified="2013-10-16" version="1">MVTTSQIYAMKILNKWEMLKRAETACFREERDVLVYGDRQWITNLHYAFQDDTNLYLVMDYYCGGDLLTLLSKFEDRLPEDMAKFYIAEIILAINSIHRLQYVHRDIKPDNLILDANGHVRLADFGSCLKLGPNGTVQSNVAVGTPDYISPEILRAMEDGQGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHRNSFEFPADETLTYQISDSAKDLMRKLICGPEFRLGQNGIEDFRNHPWFDGIDWESLRLSQAPYIPEVSSPTDTSNFDVDDNDMRLSDAVPPTANSAFSALHLPFIGFTFTQNSCLLIWEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPQ4</accession>
    <name>T1GPQ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-cell immunomodulatory protein TIP C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02174633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPQ4"/>
    <dbReference type="STRING" id="36166.T1GPQ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005593-RA">
        <property type="protein sequence ID" value="MESCA005593-PA"/>
        <property type="gene ID" value="MESCA005593"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020272_2_0_1"/>
    <dbReference type="OMA" id="PGDWIPW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057089">
        <property type="entry name" value="C2_TIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024881">
        <property type="entry name" value="Tip"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412:SF0">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23122">
        <property type="entry name" value="C2_ITFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588422" description="T-cell immunomodulatory protein TIP C2 domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="546"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="T-cell immunomodulatory protein TIP C2" evidence="9">
        <location>
            <begin position="447"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005593-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="591" mass="66460" checksum="407B45EF54F0565C" modified="2013-10-16" version="1">MKFPFYCAFLAFSSSVVSSINITNLSFGAYSGVTAAFGDFNSDELTDIFMITNNSQTLQVLFASLTEPLLREGPKCHFDRHKITSVVPGDFDGDALMDVMVTLSEHSDSDKLKVFVNWGGSDYINCSNVHEKPLLETIGEPMALDYDRNMIIDLFGLNADGIDNYSSESRTFWVFQKDRSSPLPLKMSTAGSHIKKLRIPHSNAYLDLNNDNCADLYLHTEDGVEVWYGKTEQNEGFKFGHDTFPVISGATPIFGQTIFIDLDLTGKQVSIIPICFKSDCSNSTILIPHEKNDANVDWGFIPPNKKDQYFTNTIAVRAGDYNMDGYPDLLATLKQINNPQIIKTVLLENVPCTTCKLDQYKRTFKVNWNAFGSMGNNTVAGIFYDFYNDGILDIIFIEKNGDTFKPLAFKNDLDYDSNFVKCIVLTGLENSRNSTEKGHFGKKKRHYGTNLPGPKIEYLTTTIEGNDQRGISSQIPQSSYLALQLPYSVFGLGRTPNFVESFTVGISNRTRTWTQIIPNSQMIVIPKQLDVPSTWKAQLFVTPGKLILKSLMALSGTCVLIMIVILLLYLKERREDRLERINGNNRFHYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ31</accession>
    <name>T1GQ31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02031853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ31"/>
    <dbReference type="STRING" id="36166.T1GQ31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005729-RA">
        <property type="protein sequence ID" value="MESCA005729-PA"/>
        <property type="gene ID" value="MESCA005729"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_271185_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16415">
        <property type="entry name" value="HAD_dREG-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="898"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="822"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="961"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="906"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1068"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005729-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1197" mass="137819" checksum="784429405279845B" modified="2013-10-16" version="1">MASGKLIKNLKHIRLVTFDITGTLLKFRKTPSEQYVEVAESFGYTIPPSLLSTSFVNEYKIMTTRHPNYGKTTHGMTYLDWWRVLEFKTDSSWILVDGTAELITKIRAAGITVGVISNFDGSLEEVLKNMKLPEVDFVLTSYECGASKPHPHIFQTALTLAGNVKPQHSLHIGDTFDLDYLGAKNAGWSAILVNPSDDLDRIKDHRMYNDTHVFPTLRLFLDTIFCVIFGSFIMIRGFVLAGGDFMGPQIKSPTLEIIETITEHLFRNEIFFKKVLQLMNRLELNVPSNFKLEKCFKNRCTQTENNELEEKLFLKRKATDELYKVKAKKIIESIGKIKIPDKTNKPRTNIESQTIQEKKIVLNIGEFKIPGTVAGNKEEFQRPRTPPPLELKSNQIPFSQLKDCQFLKTMKKDVIEEDLMGIYGRFAKDPSSIEIKLMQHGRMKGQAFVTFTNFTEKEFYRVEEALNSTLGFILKKKPMIIASLITRRQQELVQAHVSSEEFIPTLTFVASSEGLEMEPLEHNSSSSNNSIEEDIQEAVEESDESLPLPLDDTNSSPPSVEQDSTSHYTVPIPIEEKSTSDERFKINPDQLFGDNLLKHFKPPDPEDDESTTQDISDLKDESHESHESHEETLKGEDIEKDNSIQEITVSNHSIEMTYESIDINNESQINRILASPISEHSLKDDEELAALKERIRMLKDIQKPILPKNMILSDESDIVVKQMERVPTEFSKEVLEDITKNPALEISKVEIVAAKDLCMQALNMQLEMGQRRESNVTNESFQPSGRESLITTTSTEYRTFAEDFYGKNNDFYMEIVERDNLITQLKESLQRSLQAREDLDTRTVNLTSEVQQLRKQIQELIESKRSQIWRRGGGDQESVGQRLSEISMELVSETDDDIENPLLTDNEDRISRSSRERQLSNPPEMEFVPINKELEEFQDSLAHYEIPIYYAIRKKFDEFISQELEKLRENYESERKIAIDQIQAEKDELRLEVDRLQIVLTNTKSGSNDVEIMKKELEAIHAKEMAELSQEVFSLKSQRNGSSCESSDQEELPSETKELSPRKKSKESLYSSPSHRQITPNNDSDEETLKERELKQFYKEKIEDMNKAYDDILKQLKRYEKKYGKFEFLSETEKEAENNISTFTIVDQDELSENDIAEIQKIKDDYERRLEEQVAQAKKDFFNELAKQVQVSLAECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD0</accession>
    <name>T1GQD0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQD0"/>
    <dbReference type="STRING" id="36166.T1GQD0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005834-RA">
        <property type="protein sequence ID" value="MESCA005834-PA"/>
        <property type="gene ID" value="MESCA005834"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388710_0_0_1"/>
    <dbReference type="OMA" id="NNAFCIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005834-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10376" checksum="88682681F1C050D6" modified="2013-10-16" version="1">MDIISSQAIPGMGGTCNLPISRDDGEDAHHHHHHHHHHRSGPGHNHGNKVPYNGYNSEEYLDRLEPNGNIPSDKSNCRYGAKGSTSTNNAFCIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQI6</accession>
    <name>T1GQI6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02181907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQI6"/>
    <dbReference type="STRING" id="36166.T1GQI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005894-RA">
        <property type="protein sequence ID" value="MESCA005894-PA"/>
        <property type="gene ID" value="MESCA005894"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075842_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005894-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="12521" checksum="23112151BED6BED7" modified="2013-10-16" version="1">MTLSEYSRPNRVGYTNTQSSPQVRVSIVTLPNSILGGGQTGQQNSNSPVINGNGGDFSTTIDSQSSRFQHSFGAGPSLSIDSRLGGGNNGAAIKYGCRFIMHIRLGENHRLICVAAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQX5</accession>
    <name>T1GQX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02091268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQX5"/>
    <dbReference type="STRING" id="36166.T1GQX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006041-RA">
        <property type="protein sequence ID" value="MESCA006041-PA"/>
        <property type="gene ID" value="MESCA006041"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1012968_0_0_1"/>
    <dbReference type="OMA" id="VIMADTY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006041-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30123" checksum="3346EA201FD32E17" modified="2013-10-16" version="1">MVKVPRKPKALEDIPEEDDIGIVLKFESAEDAQKNEHKSLLSIEESLPKSPEIQYLAINGDNNFVRSASAAVVQNVHKQGENEFGVTRSRKISTPVRVPRNQTFTQGDGLQMNSQSKLNRINMLRNSSKNQLSRRSPSTSSFQYLSTPFHGSTLSFQPKEFSSHLSLASGTGAASIADNQYGTDNEPAAKEKSKFFDLSLLKDPMYLVILISNSTNAISYTNFIILLPAYGISLGFDKSLAAYLISIVSTTDLIGRIGGSALSDLNLMPKTCGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR58</accession>
    <name>T1GR58_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02004897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR58"/>
    <dbReference type="STRING" id="36166.T1GR58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006132-RA">
        <property type="protein sequence ID" value="MESCA006132-PA"/>
        <property type="gene ID" value="MESCA006132"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_1_0_1"/>
    <dbReference type="OMA" id="QWPSALM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF73">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="6">
        <location>
            <begin position="60"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="5">
        <location>
            <begin position="151"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006132-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="273" mass="30634" checksum="BFFE12CE23D1F8A9" modified="2013-10-16" version="1">MDKLSRLFNQSLRISSQIRYKSMMIEKPKPGTGVAYRRIVHYPEDGKYTVQPLKITHLAGRDPETGRLVAKGIGGGLKHKYHWIKWDREGPLEGPPQVEKVLDVIFDGCRTSKVAIVGCGDDVKYILATENMKPGDLIKTSKFIPRIPVRPNEGDAFPLGALPVGTRIHCLEKNPGQPCHLIHAAGTYGLFFREFAFDRTCMATVGRVSNIEHDKTPVGSAQKMRELGNRPRSGLWKRKEGRHGRKIRKLPPMSNITPPKAPEEKPIFLSMPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRC4</accession>
    <name>T1GRC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar pre-ribosomal-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRC4"/>
    <dbReference type="STRING" id="36166.T1GRC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006204-RA">
        <property type="protein sequence ID" value="MESCA006204-PA"/>
        <property type="gene ID" value="MESCA006204"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_353849_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039844">
        <property type="entry name" value="URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032436">
        <property type="entry name" value="URB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021714">
        <property type="entry name" value="URB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500:SF0">
        <property type="entry name" value="NUCLEOLAR PRE-RIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500">
        <property type="entry name" value="NUCLEOLAR PRERIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16201">
        <property type="entry name" value="NopRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11707">
        <property type="entry name" value="Npa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 N-terminal" evidence="2">
        <location>
            <begin position="54"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 C-terminal" evidence="3">
        <location>
            <begin position="582"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006204-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="794" mass="92104" checksum="EFA0CE03511561CF" modified="2013-10-16" version="1">MPINKRKINDESDSENEKPKKIKSQEKEEEITFHGNFNVNNFRSALRGNNFITGNMVLIRNILDKSFLINALVNGLLFDDHVTVCLVLNTLSKYVLDCQQISKTKKIQIFNIDCTKSLVKLYQWLGPKGFYQKFINKSNNFDEIVDEEEKEPVVAIVHTFLITLLTSRKYGIAFYALMNSKQKHNMIQLKIINFIDKPWLSDLKLELISKILTACPELSRNTIKHYGSLINPFKTGHNSWVAVAEFNRKLIEHCHPKLMKNALNNIALTDYCGYIKDICLPIEILTHLHGENEDIMEDELNSLVLKLLAFMFDKLYENGQDNVDVKQYFEMIFSHSKFLEVLLSTSTNIFKVKTEMLHLLTILALKNQSVLEEKQIPILLGAYDAKLTHRDRYILALLQMYEEVDINLNTYRPFIWGESAVAFYSLRDEEARNNLKHQETSISQVMSLIEFNRSDYTLKNFPVWRKLNTLKQLPNISFKDPENNLFDYGNNALEKLVERGEPISPEDLKLCPKREGIFEECYDPSFLIPLMAMSFAPDVYSQPVRPVQNGLLSIVFAGLSSLDRDMRLAAGCAQIRYREHFEASIPRVSYIPGIFLSRTFNCLVDPTHEMYKPLTMYLRLKQSFNFQCVPEFNVFFYSPEVEHQVYRKFILEVIRDGLKCNTDLFLLIANNIFKSLMGYYNISILLLPKLSRKVNLKSFSKYLNVLLKTGKIANAWKSIPEEHIVQLIDCGQKHFENYLWHIENIKHYGAKNALSFSDFVSEINSTLPISTDEDTFRIQGISSLREFVILWKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRP6</accession>
    <name>T1GRP6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRP6"/>
    <dbReference type="STRING" id="36166.T1GRP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006334-RA">
        <property type="protein sequence ID" value="MESCA006334-PA"/>
        <property type="gene ID" value="MESCA006334"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1157575_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035253">
        <property type="term" value="C:ciliary rootlet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033192">
        <property type="entry name" value="ODAD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006334-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="28554" checksum="4DE339C59042EDBB" modified="2013-10-16" version="1">MEEIFKILMRVTGALSSDEVLSRFHMQKESMTRLVYLRKTAEEEKEMLEKSRDDLMKELERTKFTDISENDINQETIEKIKKSITDLSEDKNNSQQLSRKTEFLMKSILEDISEIIYKLQEFNDGVLKTSIMDSVDNFDICLMTDAERFEKSLKELEETLAKQQSEINRLQTVHREAMDMRDNAKSVLQRQEQQSVQSNKMREKQALEFKKETRKKPLLHDKDSKKGILENILYHYRRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRY7</accession>
    <name>T1GRY7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 11">As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRY7"/>
    <dbReference type="STRING" id="36166.T1GRY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006434-RA">
        <property type="protein sequence ID" value="MESCA006434-PA"/>
        <property type="gene ID" value="MESCA006434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012393_2_1_1"/>
    <dbReference type="OMA" id="FNSYAML"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000117">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="13">
        <location>
            <begin position="6"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="12">
        <location>
            <begin position="168"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="15">
        <location>
            <begin position="289"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="14">
        <location>
            <begin position="432"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="592" mass="66209" checksum="708F46C22D401019" modified="2013-10-16" version="1">MPISSLTTGCIQSIMTGTEVEKPVLQILGTKKIASGNDSERYRILISDGKYLNSFAMLATQLNHLQQEDKLKENTIVRLERYTTSMVNKTDKADKRVMIILELTILNDGREVGQKIGNPQTFNPADAASAPAAAAASAPKPTMNNNNYAKNNNVSMNQSINSGHLISPIASLSPYNNKWVIKVRCMSKSQIRTWNNAKGEGKLFSMDFCDQSGEIRATAFRDDVDKYYDLIKQDGVYLISKCTLKAANKQFNHLKNDYEMTFGSETVIQPCEDDNDDSIPTVQYNFTPINRIAEMEDKSMVDICGVVKEIGEIVHITSKTTNKEFKKRELIVVDQSEASIALQIWGDDAVNLMVTFNPLLLLKELDSANSMELKVLALELDPFINSIQIFLSVIKFEDARTGGAGGGSMSTEWLSFFDVRARNLGNGDRPDYFQTKATIQMIRGQNAIYKACSSPDCNKKVVDEGESFRCEKCNITSHTYKARFLVNMSISDWTSNRWVTCFNDLVEQMLGKSASEVDAEIQASAGQEGNENEPFSKMFFQSYVFKLRCKVETYGDSQRNKFTAVAATPLNYKEYNKHLIGDLSRLTGIGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS91</accession>
    <name>T1GS91_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390142">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006542-RA">
        <property type="protein sequence ID" value="MESCA006542-PA"/>
        <property type="gene ID" value="MESCA006542"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2892480_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006542-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7254" checksum="E51110DB17E61003" modified="2013-10-16" version="1">CEQLIDRFFHLEKGNTVRGKNGKRKNTLHSPLNQADGSLWEVCFLLTGNMLYSVYESCVDVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSA0</accession>
    <name>T1GSA0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005796">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006552-RA">
        <property type="protein sequence ID" value="MESCA006552-PA"/>
        <property type="gene ID" value="MESCA006552"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973007_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006552-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14243" checksum="58B749EA26C2B308" modified="2013-10-16" version="1">MFPSPQNPSTGLPTNNPQRKFNTAALNMEFTKTHYEDLDTSRLEALSESPDIETGWKQGGSYAIRRFPKCSDISQGNQSQKFKNNEDIPPPTRDEVDKAIQRLTNNKSAGATSIPTKFLILDKDNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSB7</accession>
    <name>T1GSB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02073353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSB7"/>
    <dbReference type="STRING" id="36166.T1GSB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006570-RA">
        <property type="protein sequence ID" value="MESCA006570-PA"/>
        <property type="gene ID" value="MESCA006570"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_14_2_1"/>
    <dbReference type="OMA" id="MRREWFV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006570-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20798" checksum="67D3EC2DE2F810A4" modified="2013-10-16" version="1">MYQAKTSSVRSGSKKKCKNSNDWIGRCWEDDNSLSPKARRSSCNYANNRIHSKTVKYKNLSFNVWDIGGQDKIRPLWKYYYKNTEGLIFVVDCNDQDRIQQARKELSLVLMEDDIKDVPLLVFANKQDLPNALSASNLAVELQLTDNKVTSWYIQSTCAVSGQGLFEGLDWLSQEILKKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSD5</accession>
    <name>T1GSD5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02005632">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSD5"/>
    <dbReference type="STRING" id="36166.T1GSD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006590-RA">
        <property type="protein sequence ID" value="MESCA006590-PA"/>
        <property type="gene ID" value="MESCA006590"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2628946_0_0_1"/>
    <dbReference type="OMA" id="WSIAIGW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006590-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8991" checksum="E3E04A30420EB156" modified="2013-10-16" version="1">MGMELNKIVRGYWMAVWVVLTPIASVGVFIFTMTDIGQTQFGDYVFPVWADSLGWMMGACTLIPFPIFTIYQLHKHDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSJ6</accession>
    <name>T1GSJ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSJ6"/>
    <dbReference type="STRING" id="36166.T1GSJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006656-RA">
        <property type="protein sequence ID" value="MESCA006656-PA"/>
        <property type="gene ID" value="MESCA006656"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654134_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="77"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="16741" checksum="49CD8C3616707D06" modified="2013-10-16" version="1">MNSKLGYDGCAHNHGWANLSCVGKLSGGHQAAVMCVTAWEGGSPGTDFVATGSKDHYVKVFEVPSTGGTVYPLLNLEPPHYDGVQALAVANDALGADAELFSGSRDSGIKRWDLKSGELKQSLNNAHKGWVSGMAISGDVLLSSCRGGIIPYGTLKHVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSR2</accession>
    <name>T1GSR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF19 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSR2"/>
    <dbReference type="STRING" id="36166.T1GSR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006723-RA">
        <property type="protein sequence ID" value="MESCA006723-PA"/>
        <property type="gene ID" value="MESCA006723"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074913_0_0_1"/>
    <dbReference type="OMA" id="HHLEQCS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009832">
        <property type="entry name" value="DUF1397"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20997">
        <property type="entry name" value="EG:BACR42I17.2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20997:SF2">
        <property type="entry name" value="EG:BACR42I17.2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07165">
        <property type="entry name" value="DUF1397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577102" description="DUF19 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006723-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29738" checksum="ABA7FA5652F09D4B" modified="2013-10-16" version="1">MEIKLAILNLIVLVIISSANASNDLPLDLIKSHLPKEFAGANLNNFNVSDAKNLLRTKCKKNLQNNEDVDEIYNSIESATTELTECFSKLFNLTSIQEEIEIAKPIGELDTVFVKHCSHRTEAETCIVNFNSKIQPCLDREEKAGNVVMMRVVHSMFDFLCYKGGDQIALFYAEKGPECLDDNKMEIGHCLNTTLQGYSVSEGIPDMNSIVLPQLVLGPKQCTDMIELEKCIVKHLEHARKSPHLILQLLCLNLLARKPNVAIALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTM5</accession>
    <name>T1GTM5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007063-RA">
        <property type="protein sequence ID" value="MESCA007063-PA"/>
        <property type="gene ID" value="MESCA007063"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640980_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007063-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8676" checksum="8A4AFACE09518CB0" modified="2013-10-16" version="1">MKLDPAVMSRSRYAYCKGKSTESALHTKLEANVIGYADADDLSILPDILMDEVARKWSALRGGEGWNMLEIEVLKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTS5</accession>
    <name>T1GTS5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTS5"/>
    <dbReference type="STRING" id="36166.T1GTS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007116-RA">
        <property type="protein sequence ID" value="MESCA007116-PA"/>
        <property type="gene ID" value="MESCA007116"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100746_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007116-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15898" checksum="93F1AFD7C1E7F414" modified="2013-10-16" version="1">MKIYLKKFFSIKISKNLWTYVAHAFNQVDHQRLECVGPDRLCAEWVLKNGGAVEFFEIKGFLKDYNLLPPEEIQLSVKTIDASNSSIMKIGFEHLIGCRKLDKIILHNCKHLEDDGLYDFGTSVISHSMSEGMLKGSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTV0</accession>
    <name>T1GTV0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTV0"/>
    <dbReference type="STRING" id="36166.T1GTV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007144-RA">
        <property type="protein sequence ID" value="MESCA007144-PA"/>
        <property type="gene ID" value="MESCA007144"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_1_0_1"/>
    <dbReference type="OMA" id="GPRYGRK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10265" checksum="58C4A8344D2DC852" modified="2013-10-16" version="1">MAKRTKKVGIVGKYGTRYGASLRKMVKKMEITQHAKYTCSFCGKDAMKRACVGIWSCKRCKRVVAGGAWVYATTAAASVRSAVRRLRETKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUT3</accession>
    <name>T1GUT3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02064115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007503-RA">
        <property type="protein sequence ID" value="MESCA007503-PA"/>
        <property type="gene ID" value="MESCA007503"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1361813_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="22576" checksum="60F2D2B7916CD5D5" modified="2013-10-16" version="1">MTLRNTWSCVRATNGISTSFQAMRVFRQGYALPCSFLKYFIIYQTLVLITFEELESKEKTPEEEADLKAAIAKLRRALKAVQLNKLNDDELTNKACVETIADICTDQKHKFTCENTGCIFPDSSPAACTNSDEEIDAESTHIAIKTVYHNCIEAKDKPKKPPMGNLLQARRNYNSPDIIAEVIDCCKPCLIVIDGQIEVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB9</accession>
    <name>T1GVB9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB9"/>
    <dbReference type="STRING" id="36166.T1GVB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007711-RA">
        <property type="protein sequence ID" value="MESCA007711-PA"/>
        <property type="gene ID" value="MESCA007711"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008096_2_0_1"/>
    <dbReference type="OMA" id="RECAIRR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04010">
        <property type="entry name" value="C2B_RasA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05128">
        <property type="entry name" value="RasGAP_GAP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF148">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="169"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="391"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="74399" checksum="2AF09BC7226C0397" modified="2013-10-16" version="1">DAKDLQTRNNNTSAVYCTIALDQEEICRTPTIEGLAPFFGEEYEFKIPRKFRQLSIYVWDRDRHLKDNHNEFWFSLNPVDADSEVQGKARVQIVFEDGLGYLKRSEEYDDRRSLETRCWYPSKDSLESSNRSIQNDYKENNETTNNIHLHHHHHHQLYNHNQHPAIKLNSKRRNTTPDFRKSDTNGKVTVRLTECLDLAKKNGACNPFGIITATYTNNYKVVQRTKVRKKTINPVFTEQFCFDLTVNNNTNNVDSHSQPNAYNFYPIDGADLNEISISLWHSATPTFNGSGSGTFLGEIRIPMQGRQQQQSLKSDAWYYLKPRSSNDTDTPGTPFSSHQGLGTLRLKIAYTALHVFPLATYDNLMDLLMEGVEQSPITTSAVHILGQIVSNKTDIAHPLVRLFTHKKMIAPVIKQLAEYELSDLNDLTTIFRGNTLVSKMMDEAMRLSGLHYLHNTLRPIIKSIIQENKSCEIDPAKVNNKNVIQANFSNLTEYVERVFDAIISSGWNCPPILRQLFHDLRECAIRRFPNNSEVRYSVVSSFIFLRFFAPAILGPKLFDLVNEHLDENTARTLKLISKTIQSIGNFVSSRSSNQFNKEEFTGDLYKNFCKPKYVASAKKFLDEISSISDGFNNNNNNNNSSIAPAVLLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVC0</accession>
    <name>T1GVC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197357">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVC0"/>
    <dbReference type="STRING" id="36166.T1GVC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007712-RA">
        <property type="protein sequence ID" value="MESCA007712-PA"/>
        <property type="gene ID" value="MESCA007712"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1121198_0_0_1"/>
    <dbReference type="OMA" id="YQWELAT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF8">
        <property type="entry name" value="PROTEIN OPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007712-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="248" mass="29309" checksum="22B1D5CA104B09E5" modified="2013-10-16" version="1">MMTKRRTQSRKRFGSNFKQRFFQLSTNSLSYSKAKGKRPTCDIPITEIVGVERLSNNNNLKMQNIFQIILRDRERPLYVQTQNCVEEKEWIDLLEKICENNKERHTMDHLGAFVNVVAYNPYQWELATTLDPAKDLERIHQIIWDKIETLEHMYQDCAGEQRGPRKQEMTHRTYVYPKTKEDKTKFRKTIHKMISVADNLNEVHTRCNQTLLQKLLPGSKEIPIGDDNYLQMNHMNRGFASKRSMLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVZ9</accession>
    <name>T1GVZ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein TMEM132 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02081270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVZ9"/>
    <dbReference type="STRING" id="36166.T1GVZ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007967-RA">
        <property type="protein sequence ID" value="MESCA007967-PA"/>
        <property type="gene ID" value="MESCA007967"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_342335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031437">
        <property type="entry name" value="Ig_TMEM132_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055424">
        <property type="entry name" value="Ig_TMEM132_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026307">
        <property type="entry name" value="TMEM132"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055421">
        <property type="entry name" value="TMEM132_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388:SF11">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16070">
        <property type="entry name" value="Ig_TMEM132_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23487">
        <property type="entry name" value="Ig_TMEM132_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23039">
        <property type="entry name" value="TMEM132_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transmembrane protein TMEM132 cohesin-like" evidence="3">
        <location>
            <begin position="253"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein family 132 fourth" evidence="2">
        <location>
            <begin position="362"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 sixth" evidence="4">
        <location>
            <begin position="481"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007967-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="828" mass="91577" checksum="6E373DD5114C778F" modified="2025-10-08" version="2">MGSSVEVHFESADSGFFLKHPSRQGQSTIADLLTGSSSSKSKSSEDAVLSVDRFTVVQTTQPVSIRASYGPFSTKQTVPARYIIPDNTETQGDHVMVKRNCRSSGVTANKYSFRYISAYSEEHYSKGLTSSSSPISCWCRSWRSFTKAKNMCSFACAMGGRQPLKGRCMPEGEDGVCVAEVVIPSNWWPSLPPPERDIRTGLPIKVPPRSVQVSYSVFEPPPRNPEACEPKVQIQPLTTFAQVPLVNAIHPYKDLRADESLTLLIPQAPLYPMSKIHVPVFLHQQPDQSIAVFVVRARVKAGMKILGATSSSDQWNISIEKENPKHTIARVTALRKDQDPDSSSKIDESFNTDKVTEIFSWLLENWELMNTAVLTARQVSQTMKVFIVSQAGKVADVTTQSTCHAEDESVIKVSSSCSSVYVDGSEMHGSLNASIIVKYGTYVGMAKFIVWMPEFPLEVVVADFRLSQIKGWRIPDDHHFKDITHDDYFLHVESLDTDVVAFAPMLASHHPRVIAVGEGNGDLLRVTLFVSDECRSRRGLLLAKQSKQGPGPLASAVASVQVDFNGETPTKPDSVQNDGIIGRGKIYRDSGDLGDIIDSRIRITSNPMIENAAAAVTDPNSTANVYVNGYQNINTATFTKNEIRMSDVPPPIPPHISRAQPPKVEYRPPVPPHRNIGVTANTANVANGKIPSSTSSTPVQKKHSHKHHRNSHIHHNHRKSQSKNNEMIRSYERPYEPKCSDHKLMSLDNLGAEVGQFENNSPEQTYSPQDVSPSQQKKALEMHMAEKLVESTTGNNAFKFDTISPLKHNDNLDIQINASANGENYFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWH1</accession>
    <name>T1GWH1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02130480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWH1"/>
    <dbReference type="STRING" id="36166.T1GWH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008148-RA">
        <property type="protein sequence ID" value="MESCA008148-PA"/>
        <property type="gene ID" value="MESCA008148"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_664943_0_0_1"/>
    <dbReference type="OMA" id="NGEICRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000279">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052612">
        <property type="entry name" value="ANP_Clearance_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755">
        <property type="entry name" value="NATRIURETIC PEPTIDE RECEPTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755:SF8">
        <property type="entry name" value="RECEPTOR LIGAND BINDING REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588563" description="Receptor ligand binding region domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="7">
        <location>
            <begin position="13"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="414" mass="46814" checksum="EE8D50094D67B61B" modified="2013-10-16" version="1">KLFLLFKVILGGLPLAVEAVNKNPTLLKNRKLSFWPVDIGRKNGTFGYSVQPIRSMTEMRDKKVVAFIGPGDGCTSEALLAAAWNMPMISFKCSDSVVSNKEIFHTFARTLAPASKVSKSVISLLLNFHWKKFAIIAGTRPYWGPEVAKAIRDLAETEKLTITHFREVSDHIPTDSTINRMQEIVDETYETTRIYVFIGEQIGMVDFVRCLQNRNLLTSGEYMVISVDDEIYDPAVRDNTMQTRFDVHIDSNGDAEGNYTVICMLDEPGYKNNIGKMSMQPVGYFVFTTSSLPEFRYSKLDRQIPWLRGSPPRAEPVCGFKGELCPPKPVDWKYITLSVCIALVFMIAGAFLIKHYRYEQTLAGLLWKVDIKDVTFIIENIEMNGKAKNCFQPCRQSFLNGADTTKRAYTSIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX10</accession>
    <name>T1GX10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008348-RA">
        <property type="protein sequence ID" value="MESCA008348-PA"/>
        <property type="gene ID" value="MESCA008348"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375174_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10446" checksum="E65A09C0BA12281C" modified="2013-10-16" version="1">MESSIVSLSEKSSLRLDHIRSNQNLVIPTILASLVGQQQTSELPSRGLENEAKSRGSYVNGVKTKYILSSRNDANYNALDPNVNMGSHNIEVVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX89</accession>
    <name>T1GX89_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inactive serine protease scarface clip-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX89"/>
    <dbReference type="STRING" id="36166.T1GX89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008431-RA">
        <property type="protein sequence ID" value="MESCA008431-PA"/>
        <property type="gene ID" value="MESCA008431"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860229_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040973">
        <property type="entry name" value="CLIP_SPH_Scar"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18399">
        <property type="entry name" value="CLIP_SPH_Scar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588598" description="Inactive serine protease scarface clip-domain domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Inactive serine protease scarface clip-domain" evidence="2">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008431-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15695" checksum="F094F601204584F0" modified="2013-10-16" version="1">MKVERLSAVILVIFCVAVASVRGIFEIRLPVGCPAAMNCTDIRFCGLDGFISKTPVDIPPERELYRMPLSDCRDPLINLEQGKCCRDPDYVDPWPSSTFRSIVTQRPKTGEDIISVPRVKRDCTYTTQRKYIVRDLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXX7</accession>
    <name>T1GXX7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXX7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008690-RA">
        <property type="protein sequence ID" value="MESCA008690-PA"/>
        <property type="gene ID" value="MESCA008690"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008690-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="9112" checksum="7B75E5B24639F4A0" modified="2013-10-16" version="1">MIKKYNFLAYYFNTYWFTPVEIWLSKDHLEVFVPERDEPILSCESEDFSKMQYISVSHFGGTDCEYFLDCPCEIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYC5</accession>
    <name>T1GYC5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYC5"/>
    <dbReference type="STRING" id="36166.T1GYC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008850-RA">
        <property type="protein sequence ID" value="MESCA008850-PA"/>
        <property type="gene ID" value="MESCA008850"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962107_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008850-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14210" checksum="193597C09A191201" modified="2013-10-16" version="1">MDLGAPYNSMELISFIINMDAKSNLSCPLLNNCWPMLPNALNLPTKLLTALKVSHVILSKDCSQITIPSKAIEAAKKANKTPDTFYVFELLESSGICIVPGSEFGKHVGKIMLEKFQKFHAAFMDKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYK0</accession>
    <name>T1GYK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYK0"/>
    <dbReference type="STRING" id="36166.T1GYK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008930-RA">
        <property type="protein sequence ID" value="MESCA008930-PA"/>
        <property type="gene ID" value="MESCA008930"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1148349_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017125">
        <property type="term" value="F:deoxycytidyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008930-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="28072" checksum="6C2FA697650C1C45" modified="2013-10-16" version="1">MKSLNIPPHELRGIGIQITKLNEEPVKKDSTLMNMFSKVAEKQKNEPIKTKEIPTIKEVEDNSIKSMFSKVKENITEKAKENITEKVAEPLAKPKLKKKNQPTGNIKGLFQNMPSRKRTNEYLLPDDIDESVLAELPEDIRLEILQEQNNQLKLKAQKNETPVIEIFEKSPTPIEQENENKYNIFLQKDNCRLLLAWMQSTDDPEDFDVNLIIQNATDLVEKKESHVIYDPIAFICRFSYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYM0</accession>
    <name>T1GYM0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02055826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYM0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008953-RA">
        <property type="protein sequence ID" value="MESCA008953-PA"/>
        <property type="gene ID" value="MESCA008953"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172957_0_0_1"/>
    <dbReference type="OMA" id="YPDCITE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008953-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12811" checksum="5321559A5E45A109" modified="2013-10-16" version="1">MEMAKNLFGSRDHEGYVGKEEHNKQQLNDSEEEPDYPDCITEEINVIDGLRPNPGGPFSALTPSMWPQEILAKLKEPSVDEPYYQPDYQFDEFGFRVEEEDGPEQMSKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYN3</accession>
    <name>T1GYN3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02103186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103188">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYN3"/>
    <dbReference type="STRING" id="36166.T1GYN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008969-RA">
        <property type="protein sequence ID" value="MESCA008969-PA"/>
        <property type="gene ID" value="MESCA008969"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094004_3_1_1"/>
    <dbReference type="OMA" id="ITFYSWS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000044">
        <property type="entry name" value="Cofilin/actin-depolymerizing factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="8"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008969-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="152" mass="17563" checksum="85B080CDFB43CB8E" modified="2013-10-16" version="1">METHPASGVTVSDVCKTTYEEIKKDKKHRYVIFYIRDEKQIDVETVGQRDAEYDSFLDDIQKCGPGECRYGLFDFEYMHQCQGTSESSKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASREAVEEKLRATDRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ54</accession>
    <name>T1GZ54_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379965">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009148-RA">
        <property type="protein sequence ID" value="MESCA009148-PA"/>
        <property type="gene ID" value="MESCA009148"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640932_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8924" checksum="C166E1B02E1B5D57" modified="2013-10-16" version="1">MPPPTFLGLQTYLCGDVIRSKYTRIQIQSYWSSLPLKSKDLVEQCSVFSTNLTIFGNPLQDPVNLRNPQFISRIREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZH2</accession>
    <name>T1GZH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009273-RA">
        <property type="protein sequence ID" value="MESCA009273-PA"/>
        <property type="gene ID" value="MESCA009273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834078_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7439" checksum="2ECBAF8C183E71FA" modified="2013-10-16" version="1">MIVVKFAVEFIETNFITVYTNVVGIQIQGGLQSNCFAICFISILCVAHWSEQELDERGESMEHDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0D0</accession>
    <name>T1H0D0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0D0"/>
    <dbReference type="STRING" id="36166.T1H0D0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009605-RA">
        <property type="protein sequence ID" value="MESCA009605-PA"/>
        <property type="gene ID" value="MESCA009605"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2190539_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009605-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12135" checksum="64F8CE2119228653" modified="2013-10-16" version="1">NDNQYRSNDNLPILSIKSNNGYHTPGYNTTTTNLPITPDSPRSTRSAPWTRTHQRGLFGNTPSSPRSVRSAISNRNSRSSLKSNRYNRSSSVESHSSIDSSSARRNLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Z2</accession>
    <name>T1H0Z2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Z2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009839-RA">
        <property type="protein sequence ID" value="MESCA009839-PA"/>
        <property type="gene ID" value="MESCA009839"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1078856_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009839-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="258" mass="29832" checksum="39868D0955DC9881" modified="2013-10-16" version="1">MFVLSCLAYPTIRDETSANKNIDEVVKEVFEIGEMNSTYVYGVDDRRAKSFSQNFDFTVKRNSEGKLNIAVFENTNINRTVKNTTRIVNNFDFIDYGISPRKPSDYEAAKAAEVLKQILNMSPTKSELQSNYIDTLPNTESAVNEKYIVQQFPIPSHSSSSSYELSPLDLYKKMNLNVKESPISYEYNDYKYNNYHQQNSQNSNSKLNHVIAALVVDKNKNQSQNKPKPYYYNPPEIKRKYSGNQIRDVSNFNNQFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1D8</accession>
    <name>T1H1D8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1D8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009997-RA">
        <property type="protein sequence ID" value="MESCA009997-PA"/>
        <property type="gene ID" value="MESCA009997"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388705_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10493" checksum="BD701DABE6622E3F" modified="2013-10-16" version="1">MSGKSTTDQLFTFRIPEDSAKRAAEIDTMNMITNKSRQILNYKDDIDVVGRTTSSVSSKGIWHLLPISEVFIQTRNLGPGANVRNNCLMPQSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H261</accession>
    <name>T1H261_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H261"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010294-RA">
        <property type="protein sequence ID" value="MESCA010294-PA"/>
        <property type="gene ID" value="MESCA010294"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778774_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010294-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7792" checksum="A83244A429B5BE13" modified="2013-10-16" version="1">MLKFTRKLFLGQSSNSGVLKRFRCSISSYDVIIAAASRIQGVTPSTVVRLLKFIKSDKIDNTPHSYRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2I8</accession>
    <name>T1H2I8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2I8"/>
    <dbReference type="STRING" id="36166.T1H2I8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010436-RA">
        <property type="protein sequence ID" value="MESCA010436-PA"/>
        <property type="gene ID" value="MESCA010436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576575_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000036">
        <property type="term" value="P:regulation of stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9917" checksum="BEC6DC9738FD113D" modified="2013-10-16" version="1">MEGTKAQYLAAKALKKQSWRFHTKYYMWFQRHEEPKIINDDYEQTQPAHYDVVEFFQRLNTDTLFFVFYYMEGTKFWGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2R5</accession>
    <name>T1H2R5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-1,2-Mannosidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02074009">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2R5"/>
    <dbReference type="STRING" id="36166.T1H2R5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010519-RA">
        <property type="protein sequence ID" value="MESCA010519-PA"/>
        <property type="gene ID" value="MESCA010519"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624817_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF6">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE IA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010519-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="9352" checksum="9011F2346C24A282" modified="2013-10-16" version="1">MWRLTHDQKYRDWGWEAVQALEKYCRTPNGYTGVRNVYQKDPQKDDVQQSFFLAETLKVSMNFGYLECYGCDIHMLCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2S2</accession>
    <name>T1H2S2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2S2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010526-RA">
        <property type="protein sequence ID" value="MESCA010526-PA"/>
        <property type="gene ID" value="MESCA010526"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1564656_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010526-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="19332" checksum="B5F3B40FBFF27728" modified="2013-10-16" version="1">MFYVMFSDAKNALLLFLLPTYLKPTGKPQQSIATAQNNFILYTSNVAKLQISGDHPPCLIILVENDDEATEGDKEKIVQIIAYFDGNKYICSTIIEALGAVYKFYWLFGISYPKSTNNTWMVIQKYFYKMSYAGESQSPNSAKLLSALYSGLQEDEEELSAMNLDKTLENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H368</accession>
    <name>T1H368_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H368"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010687-RA">
        <property type="protein sequence ID" value="MESCA010687-PA"/>
        <property type="gene ID" value="MESCA010687"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968295_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010687-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="33700" checksum="4E06AA096C2C6354" modified="2013-10-16" version="1">MDTKVTLSNIVSSCSKEWKHPSGLELVSKAEIPRKGPALSLREINRMLMQARKTLIRYKHHNPKNMDFRDYCEVSKAWETIDRYELLEEKKHLYKELEVEENPRKYPRAPLKKLELTDRQLDKVVDYYPKTLRIMSLLEHLKRKKHLTDEDYDIRKFGAEWLLPYKQRVLDKDLKKKEEGESTRSSSKSKKYEKKSDKKADEKKSVSSKRSSSPASDKHDKKSKTEKHSTSKNTIAVSSQKPAPKEVNKTLPRKIPDKTTIVSNTNIDWELGLPGKFMSRLVSYLPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3T0</accession>
    <name>T1H3T0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3T0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010917-RA">
        <property type="protein sequence ID" value="MESCA010917-PA"/>
        <property type="gene ID" value="MESCA010917"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2055585_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010917-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13536" checksum="35723569A492940B" modified="2013-10-16" version="1">MEQSKIPKPGGNFTKIPLPSTKRSFSVANDSKIARPAKSFFGLKPPSIFQSKKLAAGEKYNSAKSFLHKKFATIDASTPSRLQRNPQQDKFYTPKEMARSDTFIREEDESPFARPQTEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4B1</accession>
    <name>T1H4B1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4B1"/>
    <dbReference type="STRING" id="36166.T1H4B1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011119-RA">
        <property type="protein sequence ID" value="MESCA011119-PA"/>
        <property type="gene ID" value="MESCA011119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_7_14_1"/>
    <dbReference type="OMA" id="FNREREQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF293">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="90"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="322"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="452" mass="51405" checksum="20B7A32FD4D6B8EB" modified="2013-10-16" version="1">MLDEFPRFSQIVFSNFSFSETSFSKTCKFMRPIYPYFNREREQEHARLHLMIQCLVGIFPSNFVTNSDPIDVNSVPSDGIQPLEIDYNDLEMQNFIAGGGFGKVYRALYFGEEVAVKEFHHSDENARKSFEHEAKIFWPLKHENIIALKGACLQKPVCLVMEYAKGGSLTTTLSGRKIPPNILIDWATQIAKGMNYLHNEAVISMSAAGTYAWMTPEVIKSGTYSKFSDLWSSSLGINHGRNSLQRIYDLTVAFGVANNTLSLPIPKTCPKPCGSLMRRCWELDPHKRPTFKEILSNLEDIQRSAFSQTPNESFQKLQNGWKKEINEVLQDLCKKEKELRSKEEDLKKVEDEQTKKEQSLQNLAKQLDEWGQKLIARELAINMSSIPNTPVPGKRFNKFKKIKLKLLKQKDIISNPSDFRHIVQAVPKQSSTPPGSPGSSIGLRVLTLFSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4J4</accession>
    <name>T1H4J4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein artemis</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA cross-link repair 1C protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02081212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4J4"/>
    <dbReference type="STRING" id="36166.T1H4J4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011204-RA">
        <property type="protein sequence ID" value="MESCA011204-PA"/>
        <property type="gene ID" value="MESCA011204"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1091044_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="13">
        <location>
            <begin position="171"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011204-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="30505" checksum="E00D71E6F38DAF6A" modified="2013-10-16" version="1">MFLFQCNDKNILYTGDFRISDFEAYSQLKDLALDKIYLDSTFLSPKFNYFPTQEESVSKICELSKRFLDKDDSNRVVILMPAKWCWLDFPFLITIYEYFNQPILVTNDVFQRYKSLEDIVVDEQQILTKDNINDDVKIILEYKGIRPTELSEQQFLGQRERELRKKYGKDLKIIIPSAFRWRNLKKDEEIVNGKHVACSFHCSFSELRNFVHHFDPEQIFLNVLTYDIIDFLKSKDLDPNNLSRENWNQLFNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4K6</accession>
    <name>T1H4K6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-B8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase B8 subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02130369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4K6"/>
    <dbReference type="STRING" id="36166.T1H4K6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011218-RA">
        <property type="protein sequence ID" value="MESCA011218-PA"/>
        <property type="gene ID" value="MESCA011218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110897_1_0_1"/>
    <dbReference type="OMA" id="IRECEGI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="14">
        <location>
            <begin position="24"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="13">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR005822-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10618" checksum="05CBA8A77333E4A2" modified="2013-10-16" version="1">MRLFLRNCAKLSSSCKEIRIHLCQTSEASKGARDFVQQLYPKVKKDNPKLPILIRECSGVQPMLWVRSEFGKETSYSLTGQKSSDILKQIESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4N4</accession>
    <name>T1H4N4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4N4"/>
    <dbReference type="STRING" id="36166.T1H4N4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011249-RA">
        <property type="protein sequence ID" value="MESCA011249-PA"/>
        <property type="gene ID" value="MESCA011249"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053932_0_0_1"/>
    <dbReference type="OMA" id="FIASHWA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="7">
        <location>
            <begin position="211"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011249-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="366" mass="43270" checksum="077C5323E869C397" modified="2013-10-16" version="1">MRSVYGDIFDSLLNIRRINLSSNKLKAFEFKSFIRTIYKSDGVFKFYEELIRKVGVTNVQLNIPTIEELRTGILKKELDLRNNKLSNLDVDFFKRIEDFDILTYLSGNDWKCNCQDLTFFEIVKNNYFIRDDITCYVNGNNEISTKSTHKDLQDICEESDYSVLVSILAPISALLFILLVMVSFKNEIKVWLFAHNMFVFWIAEDEIDKDRIYDAFICFQERDESFVEQIVNVLENGPNPYRLCLHFRDWIVGDYITKQIVNSVEQSKRTVFIITSNFLDSMWSRLEFRAACAASLREKRPRVIVILCQEMDELGDLDDEIKSYFKTNTYLKWDDKLFWSRLKYALPHKKSADSDEMEMKSLLLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4S1</accession>
    <name>T1H4S1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4S1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011289-RA">
        <property type="protein sequence ID" value="MESCA011289-PA"/>
        <property type="gene ID" value="MESCA011289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729566_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8040" checksum="9BC066FB392AA8A0" modified="2013-10-16" version="1">CLDCGVSSHDNRHLLTVDSKRKIFNENYEILNTPPSKQPISKLATHANGARTPLGNINGNKMVLRSNRQQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H579</accession>
    <name>T1H579_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H579"/>
    <dbReference type="STRING" id="36166.T1H579"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011454-RA">
        <property type="protein sequence ID" value="MESCA011454-PA"/>
        <property type="gene ID" value="MESCA011454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045161_0_1_1"/>
    <dbReference type="OMA" id="MIGRYRQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030863">
        <property type="term" value="C:cortical cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.210:FF:000003">
        <property type="entry name" value="F-actin-capping protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="18738" checksum="C494618636440475" modified="2013-10-16" version="1">MAYTANHYRHGVCAVFSRTQNGQVTLTICIEDHQFQPKNFWNGRWRSQWHATFTPGSNSAGELKGIFKVQVHYYEDGNVQLVSSKECRESIHITNEQQTAKEIIRLIEEAENEYQQAISENYQTMSDTTFKAMRRQLPITRTKIDWSKIVSYSIGKELKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5E6</accession>
    <name>T1H5E6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5E6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011523-RA">
        <property type="protein sequence ID" value="MESCA011523-PA"/>
        <property type="gene ID" value="MESCA011523"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608776_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011523-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9226" checksum="6097AC22E622F4A7" modified="2013-10-16" version="1">MKMILMALSRKRRSNLLVVRNNSNPAFRRLRKGSVGEADISITDKESIHTKTAKKETEEIARKGRFLYNLKILLWKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5L8</accession>
    <name>T1H5L8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5L8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012001-RA">
        <property type="protein sequence ID" value="MESCA012001-PA"/>
        <property type="gene ID" value="MESCA012001"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590547_0_0_1"/>
    <dbReference type="OMA" id="WNGERNS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF40">
        <property type="entry name" value="MOXD1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012001-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19067" checksum="55D5C07158B44999" modified="2013-10-16" version="1">RKEETCTVLTLYYPRQKKLTSCHSLPSLPTVLHSLGIEELAMNSNPVLISSPRELAGMTLESRLLSYDWQNQFDKFQQVTRRGSFKPLCWGAKNHLIPGSEFLEGFNVNITKSWKKPKRCKPRXFVTETTSDLDLPVFSMDTNSIIEGAARSSRSSAQRLPKAHSQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5S4</accession>
    <name>T1H5S4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5S4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012057-RA">
        <property type="protein sequence ID" value="MESCA012057-PA"/>
        <property type="gene ID" value="MESCA012057"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3420683_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012057-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="25" mass="2755" checksum="21F98259933328E1" modified="2013-10-16" version="1">SAVIWEENVVQIFATLGHVSRPTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5V6</accession>
    <name>T1H5V6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Electron transfer flavoprotein alpha/beta-subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5V6"/>
    <dbReference type="STRING" id="36166.T1H5V6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012089-RA">
        <property type="protein sequence ID" value="MESCA012089-PA"/>
        <property type="gene ID" value="MESCA012089"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_0_0_1"/>
    <dbReference type="OMA" id="DELLAXL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012089-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="13977" checksum="6B48E69B2A5A155D" modified="2013-10-16" version="1">MARVLVGVKRVIDYAVKMTAALLHWPQGTFCVKVDKTDAGISREIYAIVETIKTKMPAVHGADLRLNTPRYASLPNIMKAKNNLIKKVAAKDAVDTTPRIDVLSVEDPPVRQAGAVVLDVDFKEGGHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H623</accession>
    <name>T1H623_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM120 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="MESCA012156-RA">
        <property type="protein sequence ID" value="MESCA012156-PA"/>
        <property type="gene ID" value="MESCA012156"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048749_1_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 120 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012156-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="258" mass="30455" checksum="D5288B8F66F84E2C" modified="2013-10-16" version="1">ETNNKYLELLEQLHSRQQKCFNDIKHQRYRMGQIASSIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVWYYCTLTIREAILRVNGSRIKGWWSAHHFISTFLVSVILIWPQGHPWQLFRRQFMYFKAFTCFVQYLEFGYQKGLLYRLKALGERHNMDITVEGFHTWMWRGLSFLLPFLLVEYLFQGYNAWTLYQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6C0</accession>
    <name>T1H6C0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6C0"/>
    <dbReference type="STRING" id="36166.T1H6C0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012253-RA">
        <property type="protein sequence ID" value="MESCA012253-PA"/>
        <property type="gene ID" value="MESCA012253"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079690_2_3_1"/>
    <dbReference type="OMA" id="DITEYCH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012253-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14703" checksum="90EF5C2899812223" modified="2013-10-16" version="1">FHFGYNETILFSSWTISSVGGLIGSMVAIFLMATLYEGLKYYREYLFWKTYNLLEYRPVPTMISWNHLYQTLLHIFQVTLSFLLMLIFMTYNVWLCLAVVLGAAVGYFLFCWKKSVIVDVTEHCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6S3</accession>
    <name>T1H6S3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Uridine/cytidine kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6S3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012406-RA">
        <property type="protein sequence ID" value="MESCA012406-PA"/>
        <property type="gene ID" value="MESCA012406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021278_0_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="60"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="2">
        <location>
            <begin position="204"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="34510" checksum="51E8F436BCC40CC1" modified="2013-10-16" version="1">ILNEQQHDQALANEYNFDHPDAFDIDLLIDVLTKLKEGRKVEVPVYNFITHGREVETKTDISLRGRDLEGVLKQYSTMVKPSYANYIAPTIAHANIPRGGXXXXXXXXXXXXXXXXXXXKLLEVLAHFYANQLMPDFLYLLPNPQIKGLQTCRGEFIFYSKLVIECALRLLPFKESIVETAQVLPYNNIGVSILAGETMKVICGVCKDIRTNEPELYYLRLLKDIKDYKVILMDATMATGASEMMDIKVLLDHDVVEDNIILSSLLMTEIGVHSIDFAFPKVKIVTSALDPEINEKSYIIPGIGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H745</accession>
    <name>T1H745_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H745"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012527-RA">
        <property type="protein sequence ID" value="MESCA012527-PA"/>
        <property type="gene ID" value="MESCA012527"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_641885_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043581">
        <property type="entry name" value="Axin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014936">
        <property type="entry name" value="Axin_b-cat-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46102">
        <property type="entry name" value="AXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46102:SF2">
        <property type="entry name" value="AXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08833">
        <property type="entry name" value="Axin_b-cat_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="4">
        <location>
            <begin position="38"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012527-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="428" mass="47864" checksum="2706532EE89B676A" modified="2013-10-16" version="1">GMSHLSGKSKNDDKEGSGPRPPVPGEECCVGKSLILRLSDDLRRTIKGTIKNEIPPNCHIFDELQNEVEFTIRSEVYPNFPTSMKYIDYIQRMSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEDDDQDILDQHVSRVFKDQTPHRSPGTLSPYPPLVPPRRRHDSGILSDATGSIGGHSMKHSKSMPENSSSYTRKLTNKWPSINTDSGISLYSADTLMKYKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7D2</accession>
    <name>T1H7D2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Exosome complex component RRP40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ribosomal RNA-processing protein 40</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRP40 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7D2"/>
    <dbReference type="STRING" id="36166.T1H7D2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012669-RA">
        <property type="protein sequence ID" value="MESCA012669-PA"/>
        <property type="gene ID" value="MESCA012669"/>
    </dbReference>
    <dbReference type="OMA" id="WIKGINV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22526">
        <property type="entry name" value="KH-I_Rrp40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05790">
        <property type="entry name" value="S1_Rrp40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000112">
        <property type="entry name" value="Exosome complex component RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000038">
        <property type="entry name" value="exosome complex component RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049469">
        <property type="entry name" value="RRP40_KH-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037319">
        <property type="entry name" value="Rrp40_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21262">
        <property type="entry name" value="RRP40_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="116"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012669-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="20445" checksum="68C206489EE76364" modified="2013-10-16" version="1">LNEVVVASKAGILKQKKNTFWVDNYQKRYTPVRGETVLGVVSAKSGDIFRVDIGTHEAANLSYLGFEGATKKNRPDVNIGDIVFAKLVVANKDLEPELVCVDSMGKKGKLGVLSDGLMFSCSINLVRKIAKESCPLIPSMQDELPFEIAIGMNGRIWIKAKTIKETVALGTAILASEKASDEDTKKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7F6</accession>
    <name>T1H7F6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Tubulin-folding protein; involved in the early step of the tubulin folding pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TBCA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7F6"/>
    <dbReference type="STRING" id="36166.T1H7F6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012693-RA">
        <property type="protein sequence ID" value="MESCA012693-PA"/>
        <property type="gene ID" value="MESCA012693"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130569_1_2_1"/>
    <dbReference type="OMA" id="VIQECIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036126">
        <property type="entry name" value="TBCA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46988">
        <property type="entry name" value="Tubulin chaperone cofactor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012693-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7988" checksum="BD371072D7D2BDD8" modified="2013-10-16" version="1">MADPRIKQITIKTGVVKRLAKEKTVYKKEVTNEQNRLEKFKAQGADSHVISKQEEVIQECLMMVPDCQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7G6</accession>
    <name>T1H7G6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="T1H7G6"/>
    <dbReference type="STRING" id="36166.T1H7G6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012703-RA">
        <property type="protein sequence ID" value="MESCA012703-PA"/>
        <property type="gene ID" value="MESCA012703"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072095_1_0_1"/>
    <dbReference type="OMA" id="CYKITFE"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012703-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13531" checksum="7A1784F53C28BB5C" modified="2013-10-16" version="1">EETLYDPKLLLDLDFHKSPAKFDPFISAAAPGEPWMKVRPLQDGDFERGFIQLLSQLTDTGKITKTQFLPVTVIEDTRVNQIIGAASLIVERKFIRGCALRARLEDVVVNNTYRGKQLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAF4</accession>
    <name>T1GAF4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000210-RA">
        <property type="protein sequence ID" value="MESCA000210-PA"/>
        <property type="gene ID" value="MESCA000210"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640946_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="9083" checksum="B4F7D14FAA2F5BB3" modified="2013-10-16" version="1">MYMIQNSNRSDFLNLLYIYFSLHKNPKVIIPSDHPQTQPSSSPSSSKEEAEEEENPCSRFRNPKNKKYVYDQDLRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAJ8</accession>
    <name>T1GAJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000256-RA">
        <property type="protein sequence ID" value="MESCA000256-PA"/>
        <property type="gene ID" value="MESCA000256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309179_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577214" description="Peptidase S1 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="10827" checksum="538B18C1DC7DE8C0" modified="2013-10-16" version="1">MVRKGILVNSVSLLLTIIAGLGQKERAVHSLYINPTVNDLSVEHKLGNVKPVDIVSYLVSVRTKFKGTKTGAVGEHICGGTLLTPNKVLTAAHCFHQYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAR7</accession>
    <name>T1GAR7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAR7"/>
    <dbReference type="STRING" id="36166.T1GAR7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000327-RA">
        <property type="protein sequence ID" value="MESCA000327-PA"/>
        <property type="gene ID" value="MESCA000327"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457345_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000327-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10379" checksum="2F2E02FB7087F789" modified="2013-10-16" version="1">MDITIGILCIDISTKLRDLHRNKQYQSYIYKSYVKFLAASGGKFVPILIGKDEDYYRNEVNVDAQTFVMIFEVEEEISVIVWHLCPKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAU4</accession>
    <name>T1GAU4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAU4"/>
    <dbReference type="STRING" id="36166.T1GAU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000357-RA">
        <property type="protein sequence ID" value="MESCA000357-PA"/>
        <property type="gene ID" value="MESCA000357"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387332_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF133">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000357-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22331" checksum="3B984221ABE12BEA" modified="2013-10-16" version="1">MVGVILDSRHTNTCTLSNPSLCTPSNPSLCTPSNPSLYNPSNLAQRTILLPKAPYCMQTMLILFPPFLDIFFPAIFPEHKHHTVQYCESKDRIIMSITRKNNSNIPNGDVAKGETIASYVQPFPAKGLGYQRVVFTLYKQNAKVDFSSFKLADGENGKERLFSTRNFYKQFQDEITPAGLSFFQSSWDKSLTKFYHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBF9</accession>
    <name>T1GBF9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DONSON family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBF9"/>
    <dbReference type="STRING" id="36166.T1GBF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000591-RA">
        <property type="protein sequence ID" value="MESCA000591-PA"/>
        <property type="gene ID" value="MESCA000591"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021567_1_0_1"/>
    <dbReference type="OMA" id="MNLFANI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024861">
        <property type="entry name" value="Donson"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972">
        <property type="entry name" value="DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972:SF0">
        <property type="entry name" value="PROTEIN DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02064">
        <property type="entry name" value="DONSON"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000591-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="576" mass="65024" checksum="AC457D20C1D4E62D" modified="2013-10-16" version="1">MSSQEWKTPDEIIRLQRLNKKKKQLQARLLNNNNNTVNFEPKEKESLLEKVVSQKRKNPFGKSSKPSQNQSEPAKRQKTVHVADISEDDSLFNLISGENSSVKETPPQAAPSVPLLISKKDSISFSQESVRRLFESKSKGFLVEDEEEEDAENIVQKQTYTKHLPVDWSIKSRMRLFAEVPLPNTNLKSNQEASGITSFVRCLDLENSNSGLDISPAAKLNQCTYYWQHPHLPWMTLFPRNAKSNTLLNVASSNDSIKEALIKDWNKSFKGLFQLLRARQCPYFYLFANSFSVLFRAAGIGGKVEMNAMLTPTSRGLRNALKQEGIDFNGEETSENAIDDFEDDEEEEDEEWLESLGVDKKQIIKNIAATNARIERKKEMAEDFSDLSLVLIEGSECQALYNFLLNSKSLITNVGRLAGIPPTLLAPVAFPKASMQMLETKSSKLRMDGVDYFSIELKGVILPHVLPFVMNLFANILDPTKTFSTTVATSHCTMAFGKATQRINEDEVSKDSKKEESKESDEEKTVTVSDTIFFAENLSDSGIPMDIIKAFCRVGEHSCHLLERVCFSKEHGYSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBI4</accession>
    <name>T1GBI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IFT52 GIFT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02029602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBI4"/>
    <dbReference type="STRING" id="36166.T1GBI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000616-RA">
        <property type="protein sequence ID" value="MESCA000616-PA"/>
        <property type="gene ID" value="MESCA000616"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1187374_0_0_1"/>
    <dbReference type="OMA" id="HEFDINE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039975">
        <property type="entry name" value="IFT52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055458">
        <property type="entry name" value="IFT52_GIFT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12969:SF7">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12969">
        <property type="entry name" value="NGD5/OSM-6/IFT52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23355">
        <property type="entry name" value="IFT52_GIFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IFT52 GIFT" evidence="1">
        <location>
            <begin position="7"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000616-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="22536" checksum="95856D0285BB6757" modified="2013-10-16" version="1">MSNRTAILFDISKNELFQVSDNFKTFQRKLMAFFKVEVCDDKLTKEKLNRVDGVILAAPQNRFFDDEIKLLWEYVDNGGKLWVMSAEGGDRKMSTNCNEILEKYGTLLNNDNVVRSHYYKNFHPKEATVAGGVVCESMWRHLITQNIDKIDYDFTDEKHQILFQYPYGSTLKVSDPSVILLTTGPAVYPFNRPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCD2</accession>
    <name>T1GCD2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCD2"/>
    <dbReference type="STRING" id="36166.T1GCD2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000942-RA">
        <property type="protein sequence ID" value="MESCA000942-PA"/>
        <property type="gene ID" value="MESCA000942"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1258886_0_0_1"/>
    <dbReference type="OMA" id="HENTMNM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF3">
        <property type="entry name" value="BTB (POZ) DOMAIN-CONTAINING 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="61"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000942-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="25928" checksum="0847E02B85CB6886" modified="2013-10-16" version="1">MLAVCMSLHCSLESRDCFQGPRPLFSPRLYPFYNMYATKNLHEIPRFIHRRKHLYVNEKYTDVEFIVGEKDEKITIKAHKLFLTMASPVFEKMFYGSEAKSQPIKIKDVSPVVFEKMINFIYTENWTISDFGFENAFDLCFLAKDYELPELEERCIQILLSKIDVGNACRLYEFALPRLLSRTKTLILTETIYLSILQHENTMNMKESTFLMILEECLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCS1</accession>
    <name>T1GCS1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001091-RA">
        <property type="protein sequence ID" value="MESCA001091-PA"/>
        <property type="gene ID" value="MESCA001091"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2612869_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9078" checksum="0DE17030B89DF030" modified="2013-10-16" version="1">MSADVQHYRNYPTEIWPTVDSQPPENYLITAMATSAAAAVVENVREKEGERDEIQRPNGTPLNERTLEECWSTIQRVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCW4</accession>
    <name>T1GCW4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Short-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">1.3.8.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:43457"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02146330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCW4"/>
    <dbReference type="STRING" id="36166.T1GCW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001137-RA">
        <property type="protein sequence ID" value="MESCA001137-PA"/>
        <property type="gene ID" value="MESCA001137"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_1"/>
    <dbReference type="OMA" id="HNCLYAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016937">
        <property type="term" value="F:short-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF26">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0274">FAD</keyword>
    <keyword evidence="5 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="17">
        <location>
            <begin position="29"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="16">
        <location>
            <begin position="141"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="15">
        <location>
            <begin position="248"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001137-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="400" mass="43675" checksum="A96FF8DEA3B1D253" modified="2013-10-16" version="1">MLFLPKTIMRRCRAPITRYLSTSSQQVVHRDVKELCRKFTNTEIVPVAASLDRSGKYPEKIIKKLGDMGLMGMTVSKDFNGAEMDYTSVSIAVEELSRGCAGTGAIVSIHNILYADILNTHGTEKQKNTFLKPYTSGRPGAFCLSESEAGSDVANIQTVAKDNGSHFVLNGTKSWVTSGIEADIGIVFATIDKERKHKGITAFIVPLNSHGVEKGKPEDKLGIRASSTCQINLKDVKVPVENILGEVGQGFKIAMQQLNKARLGIASQALGIAQASLEQTVLYAKERKQFGKRLSELQAVKIRIAQMAARIEGARLLVRKATQLADEGKPFVKNAAIGKLMASETANFSANQCVQVMGGMGFVRNMPAERFYRDARITEIYGGVTDVQMMIIADQVLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD09</accession>
    <name>T1GD09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="3 11">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3 11"/>
    <dbReference type="EMBL" id="CAQQ02024629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024632">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD09"/>
    <dbReference type="STRING" id="36166.T1GD09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001188-RA">
        <property type="protein sequence ID" value="MESCA001188-PA"/>
        <property type="gene ID" value="MESCA001188"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_1_2_1"/>
    <dbReference type="OMA" id="EGVYAQF"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047975">
        <property type="term" value="F:guanosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF9">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="13">
        <location>
            <begin position="30"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="293" mass="32340" checksum="6F6EF4870EA5628A" modified="2013-10-16" version="1">MPEQTNICYTYEVCKQIADFLMQRTEFRPKIGIICGSGLGSLADQLTETDIIDYKDIPHFPVSTVEGHVGRLVFGYLGDTSVVAMQGRFHYYEGYDLAKCSMPVRVMKLIGCTNLIATNAAGGLNDKFKVGDIMIMRDHINIMGLSGIGPLIGPNDARFGPRFPPMANAYDSQYIKAAKEIAKNMNIEDDVKEGVYTCVGGPNYETVAEMRMLKMIGLDAVGMSTVHEAITARHCDMKVFAFSLITNICSLEYDDNEGENANHEEVITVAKDRQSVCAKFVTNMVKAISESIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDG6</accession>
    <name>T1GDG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001352-RA">
        <property type="protein sequence ID" value="MESCA001352-PA"/>
        <property type="gene ID" value="MESCA001352"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834080_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7583" checksum="C7DE084A9277DEA6" modified="2013-10-16" version="1">MNCKDNINLTVAAKSTPRIIHCIHTLPIVRYYKDISTFGECPVVADRRMFLCASLRYTKNKLPPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDQ8</accession>
    <name>T1GDQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDQ8"/>
    <dbReference type="STRING" id="36166.T1GDQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001451-RA">
        <property type="protein sequence ID" value="MESCA001451-PA"/>
        <property type="gene ID" value="MESCA001451"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962097_0_0_1"/>
    <dbReference type="OMA" id="MIMKKES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001451-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14649" checksum="BDE4C0E8CB5B11F9" modified="2013-10-16" version="1">MPEDHTTSEQVVLESEEDANLTDDLDETKFLVPKKDSNLDLTAKKKYVTQSKTAKPEYPFVCSCGKSYKIKGSLKRHQSYECGVIPSLTCKYCPHKSKYKSDLRKHILQKHSHLDPDVSFENTLTILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDV1</accession>
    <name>T1GDV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDV1"/>
    <dbReference type="STRING" id="36166.T1GDV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001502-RA">
        <property type="protein sequence ID" value="MESCA001502-PA"/>
        <property type="gene ID" value="MESCA001502"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015567_1_0_1"/>
    <dbReference type="OMA" id="YTEEHML"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22967">
        <property type="entry name" value="DD_AK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047499">
        <property type="entry name" value="DD_AK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="683"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001502-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="797" mass="93434" checksum="DE290780EEE52FDE" modified="2013-10-16" version="1">MMYDAVKGYCVIEDGSCKPICSTGQRKAKDDSICSNEFLVCCDNAWKIETCSSEMAEDEESVPFYKVFINYVDTYQSSAIVQELSSTKTSTFEINGSIRDQKSPLLLKGALSGNQCSYEGLGALVKSKLAREIPDAIAVSAEDESNELEQEKEEVKYLILISTPMTWAATPKKEKLSPRESEEEEEEDPNFDHILEQDYRKRRTIPSYMDQKRIERQIIWMNKKCKGKVKALVICPGVTYGGYNDVFHYLFRNAYYNNEVPLFRPGTQMTPLIHIDDFAGVIRYTIENFPPASIPYILAVQPIFFSHEEFVKTFLRAVKTDCFKIRYLDEKEILKLDTEYHCVSIFYQQYLLISFIIFQQYIIDCLRLDVKMTPTYLANFPFKVIESFKTLFDSLLDVYREARGLRNLKILIDGPPMVGKTELAKTCTYHLNHIDFEHIIHSTIEYLETKKVEASNYLTNFCPYDEIVEEEEHNLPEERVEINNLIDGWNLEINELKEYERTHKNQYYHSMDVLRKCLLTFIGPKTPNCIQGYILDGFNLFYADAKDLLFYPMESVEVVEEESVTDVDTLAAEKERKPITHCLIPTHLIILDSTDEILCNRAVALEHCEAVKRNRTEFEMLGRLEAYRDRDNDDTCLANLFYDNNMTPIYFCVRQKTRLVEILEKCKQEIGIAFVYPPTQREIDIAEKLKEQQERHQRELEEKRKNRMLEKMRCEREAKLRAWTKEYEKLKIEEDKIHEARTLPLKQYLIKYVLPTLTDGLLKVANLSLKILWTSWQNFYLRPILMVTYFLQLMKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEN4</accession>
    <name>T1GEN4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="9">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK118ub1). Mediates bisymmetric organization of the PR-DUB complex and is involved in association with nucleosomes to mediate deubiquitination. Does not deubiquitinate monoubiquitinated histone H2B. Required to maintain the transcriptionally repressive state of homeotic genes throughout development. The PR-DUB complex has weak or no activity toward 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Polycomb group (PcG) protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, at least composed of caly/calypso, Asx and sba (MBD5/6 homolog). The PR-DUB complex associates with nucleosomes to mediate deubiquitination of histone H2AK118ub1 substrates; the association requires the positively charged C-terminal tail of caly, probably due to direct binding of DNA. Interacts (via ULD domain) with Asx (via DEUBAD domain); the interaction produces a stable heterodimer with a composite binding site for ubiquitin. Homodimerizes (via coiled-coil hinge-region between the UCH and ULD domains) to mediate assembly of 2 copies of the caly-Asx heterodimer into a bisymmetric tetramer; dimerization enhances PR-DUB association with nucleosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 12">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="9"/>
    <dbReference type="EMBL" id="CAQQ02102279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GEN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001802-RA">
        <property type="protein sequence ID" value="MESCA001802-PA"/>
        <property type="gene ID" value="MESCA001802"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_0_1_1"/>
    <dbReference type="OMA" id="YIQYEIQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000027">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.860:FF:000001">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52049">
        <property type="entry name" value="ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 9 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="13">
        <location>
            <begin position="7"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="11 12">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001802-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="40445" checksum="B4E3C5518E3F30A9" modified="2013-10-16" version="1">MGESAGNWCLIESDPGVFTELIREFGTEGVQVEELWSLDQEAFKNLEPVHGLIFLFRWVQDDEISGTIVEDDRLEKIFFAKQVINNACATQAILSILLNCQHEDLXEKIFFAKQVINNACATQAILSILLNCQHEDLKLGKTLSEFKEFCQSFDPHTKGLTLSNASQIRTVHNSFARQTLFELDNSNLNKDEDVYHFIGYIPIDGRLYELDGLKEGPIDLGAIESGKNWLEVVKPIIEKRMNKYTEGEIHFNLMALVSDRQLIYQKEIDRLSASDAMDSDDRDIEIARLRSLIDDEIAKKKRYKVENIRRKHNYLPFIMELLKMLGEQGQLTPLYEKAKQRAAERENSKTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GER5</accession>
    <name>T1GER5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GER5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001836-RA">
        <property type="protein sequence ID" value="MESCA001836-PA"/>
        <property type="gene ID" value="MESCA001836"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_804854_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001836-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="345" mass="40582" checksum="74AF25C638465C90" modified="2013-10-16" version="1">MFDNRIKEIERERRLILEGKTDKVPDELAMNPYSWSTSKSKLKTTREKNAEKLLKQGAKWERENVIYAEQQKLKEAEIKARKKAREQLIELYRKEDAVRGLDLLRFEDLTFDDWRECELDLKNFLETERAKMKARSEQVPTPYLSDPLDIMNIMRARKEAEEKARKEKEEEERIRREKEEAERLRLEQLELEKKAKKGKRKVKAPVPITPPTPEPPLEGEALLKANLSFDEDDQQKKLGDESCQTIISTFPGQEGPTENLAARLRDLKDSVVQDTQTGYLNAKETYNPYFYVDPKINRLQAAKNMVELFRIAEEIIMGDAFIEVEENLEEEYVDEVEEPKSYEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF41</accession>
    <name>T1GF41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S16</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024142">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF41"/>
    <dbReference type="STRING" id="36166.T1GF41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001970-RA">
        <property type="protein sequence ID" value="MESCA001970-PA"/>
        <property type="gene ID" value="MESCA001970"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1596388_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001970-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="19915" checksum="DEB6EDF17C5A3ED1" modified="2013-10-16" version="1">MEDNKMLRPRYRLVSESTLEPFSSEFFENMQQKHKEPIHAVRRWWYQIYAIRQAISNALVAFCQKYVDEASKKEIKDILIQYDRTLLVSDPRRCEPKKFKKKYKKKFTFSLARTFLSLDAPLLSSIFTQKDFHESDIPALLLLFISFNFPKFVRSPDNSCLFALQFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFN1</accession>
    <name>T1GFN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFN1"/>
    <dbReference type="STRING" id="36166.T1GFN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002176-RA">
        <property type="protein sequence ID" value="MESCA002176-PA"/>
        <property type="gene ID" value="MESCA002176"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2090800_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002176-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="14661" checksum="9078FB904AE8CB56" modified="2013-10-16" version="1">MIKHPKYIGSGYSFDIGLLKLNKNIKFNDFVKAIKLPPQGHLLYENCTAIATGWGKLSDSNPKSKKLQFVPLKVTNIKECDTFYRKTFGLEILCTSTKGKKSTCSGDSGGPLVYYNQVLIGISAFVPTFIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFR3</accession>
    <name>T1GFR3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFR3"/>
    <dbReference type="STRING" id="36166.T1GFR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002210-RA">
        <property type="protein sequence ID" value="MESCA002210-PA"/>
        <property type="gene ID" value="MESCA002210"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973026_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="13563" checksum="9AFCF25EC803298E" modified="2013-10-16" version="1">MEENNESSITEAVEKQLNASQATPQMSKKSNGTSSATSTLSTTGNNDIVMTPLSDCGTNSTKLSTFSSQGKGNVQMRRTTNKQGKQAPAPPKRTSLLSSRDSTYRDEDDLMKSSSNLLSYATNGNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFW6</accession>
    <name>T1GFW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02078665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFW6"/>
    <dbReference type="STRING" id="36166.T1GFW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002268-RA">
        <property type="protein sequence ID" value="MESCA002268-PA"/>
        <property type="gene ID" value="MESCA002268"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024950_1_1_1"/>
    <dbReference type="OMA" id="MPACTSV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002268-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="398" mass="46852" checksum="C8D856B09B9701EB" modified="2013-10-16" version="1">MIIGFSEKDNSIYEIPFPAVTICPEIKTRSYKLNYTDLYLKYIKGNNLPEKPIEFSSLLEICKKTPKFKNFTNTRYSLDEYVNSLQEIGPEFKETITECGWLGEKEDCNQMLTKVLTEEGICYTFNGLDKNEHFAKYVHLPTSIQRSIHRITNWSLDYGYDDCQNVYPRREYRACPLWEYTSNLRLIDLRDTPQVHKNYFKIHMNRDVIVAVKPIVTVTSSNLYNYDSQRRNCFFQNEKYLRYFKMYTQDNCDLECHTNFTLDRCDCVKFTMPRNESTPVCGPADFQCTELSDTIFFKNFLKDCNCLPSCTTIEYDTKRSYNVVEDDSNISTSTVRVFFKQSNFFAMRRSELFGQTDLLANIGGLLNLFMGVSLLSVIEFIYFFTIRLLSHQFQRNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFX1</accession>
    <name>T1GFX1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dipeptidylpeptidase IV N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFX1"/>
    <dbReference type="STRING" id="36166.T1GFX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002273-RA">
        <property type="protein sequence ID" value="MESCA002273-PA"/>
        <property type="gene ID" value="MESCA002273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1014015_0_0_1"/>
    <dbReference type="OMA" id="RELWNIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF192">
        <property type="entry name" value="IP17501P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="1">
        <location>
            <begin position="59"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="275" mass="32555" checksum="3CEF9D3736B7183A" modified="2013-10-16" version="1">DILSGKFNIKRFNGTWRNDESIIYKDRSGFLLKYNVHENRADRLNIKEIQFHYVSYEVSADEQYVLLVKNYIKIFRHSFLAQYDILNLQTGIITELTINNKQEYLLMATWSPVGNALILNYDRNLYYKSSVFGKEIQLTNNQYFNGIPDWVYEEEVFNSNVAVWFSPDGNRIAFIQFDDSQTSLINIPIYGESGNMNFQYTKNRPIFYPKAGSKNPFVKLFYINLNDTHFLPYQIQAPEELNTSEYIITVVSWVNNYKILSIWMNRVQNMAIYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG48</accession>
    <name>T1GG48_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA002357-RA">
        <property type="protein sequence ID" value="MESCA002357-PA"/>
        <property type="gene ID" value="MESCA002357"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362111_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002357-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11001" checksum="2C6E8F5407E030D7" modified="2013-10-16" version="1">MGNVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISPKIGRSCSSTIYAANVSFIGIEKVFKFRESRNIRRTSQEMQRCHGDAFRRRQGDPQQRLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGQ6</accession>
    <name>T1GGQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGQ6"/>
    <dbReference type="STRING" id="36166.T1GGQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002582-RA">
        <property type="protein sequence ID" value="MESCA002582-PA"/>
        <property type="gene ID" value="MESCA002582"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309197_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002582-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11669" checksum="34EC24FA07C3DEFE" modified="2013-10-16" version="1">MYVQGKAILFTTDIPNEKMECGKIEGVFATTDFGVDGIYLLYIINTLKQSNERINTNLVQRRKIGKTKKNLGVWSQCQHIHVLWWEQFRVLGGGQRHWCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGT1</accession>
    <name>T1GGT1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02075601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGT1"/>
    <dbReference type="STRING" id="36166.T1GGT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002612-RA">
        <property type="protein sequence ID" value="MESCA002612-PA"/>
        <property type="gene ID" value="MESCA002612"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_819619_0_0_1"/>
    <dbReference type="OMA" id="WECIYNS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038877">
        <property type="entry name" value="THSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16311">
        <property type="entry name" value="THROMBOSPONDIN TYPE I DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16311:SF3">
        <property type="entry name" value="THROMBOSPONDIN TYPE-1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="49"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002612-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="339" mass="37822" checksum="F3059B16F285D19F" modified="2013-10-16" version="1">MIGPSVETQQCGQPMPDWECIYNSGYFDSGNEILAHPGISEEIGPGCRCGCIVHLAKRMIASSTESCPGKSFWLIQADGPNTRIRMKINYFRLPCEKQYLKIRDGDSVSSNLLSEKLPGIQNFSAPVTSSGHKILIELFSEKKISEDSVCSGGFLAHVELIEEPKEKVIYRSSFSRKVLTQKSRISKDFTLVHVVAVIFAGFIIIISALLGAQYILRYRKYHLTISQNDTDSSPLHTPRGSVGSLQMQPSPPSRALSTTTLLSEVIYLVKLRTKTNNVKHSILRESVDAEAAILTKETKFNDPSTKKKKTIHSNRNNRKLTLIYCPILILVFLNETNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGV1</accession>
    <name>T1GGV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02075505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGV1"/>
    <dbReference type="STRING" id="36166.T1GGV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002632-RA">
        <property type="protein sequence ID" value="MESCA002632-PA"/>
        <property type="gene ID" value="MESCA002632"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069908_6_0_1"/>
    <dbReference type="OMA" id="CNITLVH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF33">
        <property type="entry name" value="PROTEIN TAKEOUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002632-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="16812" checksum="B48935D15DBF00B0" modified="2013-10-16" version="1">PPYNYSLDLKNHNLTFDIDLPLLRIRAKYNLKGNILLLPLVGNGDVNMALKNVQTSVLTKISFRDEPEETIHIDSMKVYFNVGGMRIHLTNLFNGNEILGASINSFLNQNSDEIIKELRPDLEAGLAETFKGLWNDVFSKIPTKLWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GID8</accession>
    <name>T1GID8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GID8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003212-RA">
        <property type="protein sequence ID" value="MESCA003212-PA"/>
        <property type="gene ID" value="MESCA003212"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815389_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003212-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7468" checksum="4EB6B7CFC21055CB" modified="2013-10-16" version="1">MQLPKPAKNSSTRVLAPPKNGIVPQRMCWLATSATILQQRYLTGSNFSVAYPQKLIHSLQENGEKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIN3</accession>
    <name>T1GIN3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121375">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003316-RA">
        <property type="protein sequence ID" value="MESCA003316-PA"/>
        <property type="gene ID" value="MESCA003316"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888331_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003316-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7052" checksum="B02BEC056957A41B" modified="2013-10-16" version="1">MLVDCGNGNPDHGKNRKRKNTLRSPLNPDEQQILVISHLELKSPKTTFMSYAEMGEYALSSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIP7</accession>
    <name>T1GIP7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02186952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIP7"/>
    <dbReference type="STRING" id="36166.T1GIP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003331-RA">
        <property type="protein sequence ID" value="MESCA003331-PA"/>
        <property type="gene ID" value="MESCA003331"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375175_0_0_1"/>
    <dbReference type="OMA" id="LWIRTTA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF360">
        <property type="entry name" value="CHITINASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003331-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10906" checksum="2153BD20ED5D462C" modified="2013-10-16" version="1">MDLYFDFGEQIYIQDLIGLKKINPNLKAIAVVVGHKKDTAKYTRVAADPKKRRNFIESAIALVQKLNFDGLDLDWEYPGTSLQDKANFDTWIKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ63</accession>
    <name>T1GJ63_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02097871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02097872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003507-RA">
        <property type="protein sequence ID" value="MESCA003507-PA"/>
        <property type="gene ID" value="MESCA003507"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375156_0_0_1"/>
    <dbReference type="OMA" id="KTMIMMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003507-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="11975" checksum="39E0C29958A2A3E4" modified="2013-10-16" version="1">MNMTRKMKMMNMLTMMRRKMKKKLPKKQLLKKKMMRKMKRQLLKNNLKNMKPQLKRRKTKMKKALLKKMKTVTMMKKEPRKTMIMMRKMMMFLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJM1</accession>
    <name>T1GJM1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003671-RA">
        <property type="protein sequence ID" value="MESCA003671-PA"/>
        <property type="gene ID" value="MESCA003671"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662808_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051520">
        <property type="entry name" value="Elbow/Noc_ZnFinger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN ELBOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522">
        <property type="entry name" value="ZINC-FINGER PROTEIN NOLZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003671-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="17421" checksum="99AAECCFB50FE658" modified="2013-10-16" version="1">MKAKAAKNASKKLTKKAKYTEQKHKINIKRFEAKRHPLYGTLGGRKKREEFSKQKIAHTLDTKNSPLALLAQTCSAIGTDSPNPKLLAHIEKSTKSSSASSTGGREKSSPVSSQSSLSSGSMSDMKSSFKPYESNNNHIPNKERVNLSPMSVIDCQMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJQ6</accession>
    <name>T1GJQ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003706-RA">
        <property type="protein sequence ID" value="MESCA003706-PA"/>
        <property type="gene ID" value="MESCA003706"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534291_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20379">
        <property type="entry name" value="Tudor_dTUD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003706-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20177" checksum="6E7321FFA22779DB" modified="2013-10-16" version="1">MNSRNLSIPSIQNWLERLNNVLENYDYDLKTAATIQDIPKPGLCSWSDNMKLANYNTNSIIAEDISASEVNRIPEITVIPSGSKVTIMDVNQANDTVYVRGCSEKENEIFLENIFRVHIAHLNAKPFENFARINNMCVTQFKKDDIWYRARVIKIIETFTYLVRFVDFGAVEKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKE4</accession>
    <name>T1GKE4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKE4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003967-RA">
        <property type="protein sequence ID" value="MESCA003967-PA"/>
        <property type="gene ID" value="MESCA003967"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729578_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003967-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8024" checksum="785942FB49120D7A" modified="2013-10-16" version="1">GKSEHLQWDLHDITNGIGLRVISFATSRNTVVKNTDGTPVELLKTAGASFNRIFHESLTKIWSIARFKNGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKF1</accession>
    <name>T1GKF1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02033038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033039">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033040">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKF1"/>
    <dbReference type="STRING" id="36166.T1GKF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003974-RA">
        <property type="protein sequence ID" value="MESCA003974-PA"/>
        <property type="gene ID" value="MESCA003974"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2102979_0_0_1"/>
    <dbReference type="OMA" id="DERACMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="6"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003974-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="12766" checksum="9771DCE01F9FE7E6" modified="2013-10-16" version="1">DHKTQCQVHQFRCDSGQCIYPSQLCDGEDDCSDGSDEKNCEKKQIVCGKNMFQCTSGTCIPKSWECDGKLDCPDTSDEHAQCSTNLQADYLPRASILFTALASTPVQDYVVGVNIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKN4</accession>
    <name>T1GKN4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004061-RA">
        <property type="protein sequence ID" value="MESCA004061-PA"/>
        <property type="gene ID" value="MESCA004061"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461113_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004061-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10274" checksum="9F1936649400B72E" modified="2013-10-16" version="1">ISNFDLNIKSIENLNISYNGIKKLICPPKNVTTALKNIWLEGNKFNDENFKISKKCIQGKVRDGLDNDSYKSSDYDSLWFKGFEFIIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKV0</accession>
    <name>T1GKV0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004131-RA">
        <property type="protein sequence ID" value="MESCA004131-PA"/>
        <property type="gene ID" value="MESCA004131"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_807231_0_0_1"/>
    <dbReference type="OMA" id="QERNKCH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004131-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="344" mass="38392" checksum="5474BC08A7E72EFE" modified="2013-10-16" version="1">MQQTFPKSEPEDKKPEPEKQTNVTNTNVTKNRRKATHTVLKEEPSTKSPPPKTPFPPVAPKKVTSTAPGGPLKQIVMNKKVITSPPNANKKMSVTPSQIHKPPVRILNSTLCRQQPPVSPIINAQKQQQAPVAAPAPVPAPQPQPPKKEHQIKKSLSPQVQNNKRLIAPTSIQKRKLPEEKITVQKQGSRTIKKMTCFENWYVVHCAANPIPQKPAPNQLTLPLIKIANGLQDICLPSEKWTSKVALSLIPESVYQRGQFERYTGHYHDKSIVKDDIKHRYQPASILFRRSQKPNVIDRKISNLVGSPKIVKDVEDIESLLDCVDHLELNSEDVDVSPSIPNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL51</accession>
    <name>T1GL51_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL51"/>
    <dbReference type="STRING" id="36166.T1GL51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004241-RA">
        <property type="protein sequence ID" value="MESCA004241-PA"/>
        <property type="gene ID" value="MESCA004241"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1789059_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008255">
        <property type="term" value="F:ecdysis-triggering hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018990">
        <property type="term" value="P:ecdysis, chitin-based cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006825">
        <property type="entry name" value="Eclosion"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04736">
        <property type="entry name" value="Eclosion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004241-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16629" checksum="76246111A751EDFA" modified="2013-10-16" version="1">MRMQQKKHHNLINLAIKVETLSQKKSVFHKLLPKNHNNMLSKTIVSIFVLTLVAAATSIESSDLFTFEPSSTNSFTYTKRFDPMSGLDFVSICLSNCSRCKQIFGEFFLDRKCEESFSSIKDKLSQTVRTIILLLLLSEFEEIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL87</accession>
    <name>T1GL87_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL87"/>
    <dbReference type="STRING" id="36166.T1GL87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004282-RA">
        <property type="protein sequence ID" value="MESCA004282-PA"/>
        <property type="gene ID" value="MESCA004282"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2190545_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004282-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12426" checksum="7D478A0DB29C3E35" modified="2013-10-16" version="1">MARLTLPRTILKACDHERTLYTFKENNLENDPLGSNGSNGCEKPFVISPIQRSNLILLVIDTMCPRDTQHFVLTDPVEIKYNESLACEKKNTILSRRKPVNCTNNHVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL89</accession>
    <name>T1GL89_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004284-RA">
        <property type="protein sequence ID" value="MESCA004284-PA"/>
        <property type="gene ID" value="MESCA004284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838561_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7530" checksum="503E441E01E92374" modified="2013-10-16" version="1">MSKDVVDEYIRSMSRSANKTVKNGRIIEKIPQWTNTIDKNDTHSVRSVPVKSKRQVMLIPAQSQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW4</accession>
    <name>T1GLW4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription factor IIH subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">General transcription factor IIH polypeptide 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with RARA; the interaction requires prior phosphorylation of RARA on 'Ser-369' which then enhances interaction of RARA with CDK7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TFB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02098498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLW4"/>
    <dbReference type="STRING" id="36166.T1GLW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004529-RA">
        <property type="protein sequence ID" value="MESCA004529-PA"/>
        <property type="gene ID" value="MESCA004529"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040211_1_0_1"/>
    <dbReference type="OMA" id="QGCDITS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004529-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="28127" checksum="5725FFE4BD8A1760" modified="2013-10-16" version="1">MDAVVAFGNAHLMQKSQNKLAVIACHHSATEFLYPTTGKMEVRQIDGQYEIFTLVEKTVKHRLAELINSASRIVQPCESLLAGSIAMALSYIARVNRNKSAGGRLASRIFIMSGSNECASQYMTYMNVFFTAQKQGVTIDVCALDKSLSLLQQGCDITGGQYLRLPQMDGILQYLLWVFLPDHHTRDKLVLPPASKVDYRAACFCHRELIDIGYVCSVCLSIFCKFTPICGTCQTVFKNTAPLREKKKKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM24</accession>
    <name>T1GM24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Short coiled-coil protein A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Positive regulator of amino acid starvation-induced autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCOC family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM24"/>
    <dbReference type="STRING" id="36166.T1GM24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004592-RA">
        <property type="protein sequence ID" value="MESCA004592-PA"/>
        <property type="gene ID" value="MESCA004592"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130081_2_0_1"/>
    <dbReference type="OMA" id="LALMFFH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000038">
        <property type="entry name" value="Short coiled-coil protein a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019357">
        <property type="entry name" value="SCOC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614:SF0">
        <property type="entry name" value="GEO08385P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614">
        <property type="entry name" value="SHORT COILED COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10224">
        <property type="entry name" value="DUF2205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004592-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9991" checksum="8DA2601724857CF9" modified="2013-10-16" version="1">MALNDDNIPLADEDVQGDSPDEKEEKARLITQVLELQNTLDDLSQRVDSVKEENLKLRSENQVLAQYIENLMSASSVFQSTNSPNGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM31</accession>
    <name>T1GM31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02134531">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM31"/>
    <dbReference type="STRING" id="36166.T1GM31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004599-RA">
        <property type="protein sequence ID" value="MESCA004599-PA"/>
        <property type="gene ID" value="MESCA004599"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877841_0_0_1"/>
    <dbReference type="OMA" id="NIWFRND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF211">
        <property type="entry name" value="PROSTATIC ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004599-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16560" checksum="FCA454CCE3BD6EDA" modified="2013-10-16" version="1">MQRLTDLSYVLNAKTEEMQKLKSGPFFSKMVTEMVKKSMNPSSKQKLFIYCGHDVTLISLLSALNAWPGVFPDYALQAYFELHRNKNGDYFVQVLAKNGVDARLERVIVPGCEYRCPLDQFVDILEKFTKVDLKEDCKPKENVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMW6</accession>
    <name>T1GMW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02048028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004900-RA">
        <property type="protein sequence ID" value="MESCA004900-PA"/>
        <property type="gene ID" value="MESCA004900"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443350_0_0_1"/>
    <dbReference type="OMA" id="CGAGNIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588395" evidence="1">
        <location>
            <begin position="17"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004900-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="8503" checksum="D961171B17F8CC88" modified="2013-10-16" version="1">MKVILVILIVISVALATPLGIGIGRFGGLGGYGGYGGYPYGGYGGYGGIPYGGYGGIPYGGGFYPGGFGGGFGGSSAFASASAGGIGFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMY7</accession>
    <name>T1GMY7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004924-RA">
        <property type="protein sequence ID" value="MESCA004924-PA"/>
        <property type="gene ID" value="MESCA004924"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725094_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004924-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8372" checksum="1829B69A419E307B" modified="2013-10-16" version="1">MSIYQRSDIVVRTKTVADNEQGECLIQYALIFLQRTTEQQRNIFEIYSFKCGFMVGLLAELFGVFIVKRTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNC8</accession>
    <name>T1GNC8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005075-RA">
        <property type="protein sequence ID" value="MESCA005075-PA"/>
        <property type="gene ID" value="MESCA005075"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760728_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005075-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8172" checksum="329E9374D5711CEA" modified="2013-10-16" version="1">MLPTFLHTVKNFIKPTIIKVTIPVFYLLLQLSPNGAFYSTDPYEFKPKLLKIFYDSLMHTNGVKMIDHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNC9</accession>
    <name>T1GNC9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153531">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNC9"/>
    <dbReference type="STRING" id="36166.T1GNC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005076-RA">
        <property type="protein sequence ID" value="MESCA005076-PA"/>
        <property type="gene ID" value="MESCA005076"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075165_6_2_1"/>
    <dbReference type="OMA" id="FNYDPHP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF95">
        <property type="entry name" value="CUTICULAR PROTEIN 76BD, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005076-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10454" checksum="6C6BE1C0BF7CFC3A" modified="2013-10-16" version="1">MILCFTSTPQDSHPRYAFEYGVNDQHTGDIKHQKEERDGDVVKGEYSLVEPDGNVRTVKYYADWDTGFHAQVINSRDQVHGKIIAKRDIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP97</accession>
    <name>T1GP97_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP97"/>
    <dbReference type="STRING" id="36166.T1GP97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005419-RA">
        <property type="protein sequence ID" value="MESCA005419-PA"/>
        <property type="gene ID" value="MESCA005419"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_792963_0_0_1"/>
    <dbReference type="OMA" id="ASSECKM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000103">
        <property type="entry name" value="FEZ family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000251">
        <property type="entry name" value="FEZ family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000164">
        <property type="entry name" value="Fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF35">
        <property type="entry name" value="FEZ FAMILY ZINC FINGER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577525" description="C2H2-type domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005419-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="350" mass="38620" checksum="1E8CCBF37F3DF0EC" modified="2013-10-16" version="1">MFKVKFKFFETSLVFTYLLLLSPRGMQPCSPSGVEEMMHPSKSPFMESSSSQQSEQSTTSMDTLKPLSQRSGPLKFSIAKIMEPDEKSSVSICDLDGERTNASTPETDGGENGNDYSAFKKYVPNSTSVVTTTVQQFVNTRHQELISQYPLLYYPGQLMCAAAQYAAITQQPLLNSGSHIPNFSASINTLQAHSIRRNLSLSTVPSINLPTSPRKVLTPPKPKSSLSSPSSEESPSNCSSSNKQKTFSCLECGKVFNAHYNLTRHMPVHTGARPFVCKVCGKGFRQASTLCRHKIIHTSEKPHKCHTCGKAFNRSSTLNTHTRIHAGYKPFICEFCGKGFHQKAITKTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPF2</accession>
    <name>T1GPF2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPF2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005481-RA">
        <property type="protein sequence ID" value="MESCA005481-PA"/>
        <property type="gene ID" value="MESCA005481"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362096_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005481-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10988" checksum="A577FF7004D317F3" modified="2013-10-16" version="1">MELQYNLPIFKKGDRSELKKYIGISLLNVLYKILASIISERLKPPALLAHINAVLCLENRPTTNFSHSDRSLKKPKENRFPLSGYCLQTDDQTITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPG0</accession>
    <name>T1GPG0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the junctophilin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02064637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064638">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPG0"/>
    <dbReference type="STRING" id="36166.T1GPG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005489-RA">
        <property type="protein sequence ID" value="MESCA005489-PA"/>
        <property type="gene ID" value="MESCA005489"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008078_0_0_1"/>
    <dbReference type="OMA" id="IDCENAV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000001">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000013">
        <property type="entry name" value="Putative Junctophilin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF16">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="181"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="460"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="640"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="510"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="656"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005489-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="861" mass="96817" checksum="63604BEEC4B5D5A4" modified="2013-10-16" version="1">MQDQQSILKPPPSNLEPSLNRRSQKSGGRFDFEDGGTYCGGWEDGKAHGHGVCTGPKHQGAYAGAWNYGFEVSGIYNWPSGSHYEGQWQNGRRHGLGVERRGRHVYRGEWSNEGQKGRYGVRQSTTSTAKYEGTWNKGFQDGYGVETLPMEWLEGKRHGHGIRTSAPFGLASHYKTKNMATSMSSLRSNEDTGDKRSTRIEESRGGFVLTGHSGDTLTRRNSFDDKSKKGFLSGLKKKQKSAGDLDKRGTMTSGSIRSTTSAVSWLSTGSSHSNMTARSVHTESNASFSLEEDLLDPSITETYSGEWKKDKREGFGIAERSDGLKYIGEWSNNRKHGYGITIFQDGRIEEGKYKNNILITKAFILFTIKKRKFCTTSFEKALQRADIAISRQGTAQTRAEEADHQAEIARIDCEHAIMAAREFSPDFKPAVLERFEKLRYRNTYLPKYEMNNTIQSSQNQLYYKQQQQQQRDKIESHPIPDTMYSQKPAVSPQTDLSQFVSNQQALNKKQQAMMSSLSSSSMHQQPPQSPSTVSTSINAFQNQNNELINNKPDKVLNVYPDRKENVLNLQTSTSHNENIINNNNIYMDPQIHIKDVPNATDEKSLHQFRKSSLHNVKPPTLKNINNNQQSSIDFYDHYKRPPSRDSSIDRYTRAASRLSGGSRLTSRQPSIDKTNIVTEPITSSTPVRKSSAEAPATNNITKNAIGSMNGKYHAPFEDIILRQRTLGQDIIPSPLQPKRTESLFVPPKPAGQNVTVSKVLKNMEWFPQDDEEEEISFSPALLARRASESWIDTSLSVESANIAVNLNRKKSLPDFQGLPLPSDTMSREEVSALGSARREEVRRLDFKAKTGISGTIRQHFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPQ6</accession>
    <name>T1GPQ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02030828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPQ6"/>
    <dbReference type="STRING" id="36166.T1GPQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005597-RA">
        <property type="protein sequence ID" value="MESCA005597-PA"/>
        <property type="gene ID" value="MESCA005597"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131137_0_0_1"/>
    <dbReference type="OMA" id="CASACNQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF105">
        <property type="entry name" value="DLIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="86"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="88"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="14517" checksum="4912BEE79C208AEB" modified="2013-10-16" version="1">MGSASEDEDDDDQHIRGSSLGLGVLGPNGPDSAGQLGNSDLSLQSMSTDSKHTHDDSDNGSLDGDPDGRGDSQAENKSPDDGANGSKRRGPRTTIKAKQLEVLKTAFNQTPKPTRHIREQLAKETGLPMRVIQVSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPS5</accession>
    <name>T1GPS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIH1D1/2/3 CS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02099758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPS5"/>
    <dbReference type="STRING" id="36166.T1GPS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005617-RA">
        <property type="protein sequence ID" value="MESCA005617-PA"/>
        <property type="gene ID" value="MESCA005617"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106090_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026697">
        <property type="entry name" value="DNAAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21083:SF0">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21083">
        <property type="entry name" value="TWISTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="3">
        <location>
            <begin position="85"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005617-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="21880" checksum="D94B14430486B8CC" modified="2013-10-16" version="1">MSIFDNPGYLKSLQDLLNPKRDEDSSSDSEDENINSVEKKPLLGFVNKTETKEVKNNGKLAKETAESWLEKQQQEDELLLETRKTPNYKMSYKQSVGTEDVFLQMGNRSASSISCEDMVITIELPEEIVPVEQMNLDISENDIDLQTPVYRLKVPLVHPIDPDLGTAKYDSETKILILTLKMKREFDFVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPV9</accession>
    <name>T1GPV9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPV9"/>
    <dbReference type="STRING" id="36166.T1GPV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005654-RA">
        <property type="protein sequence ID" value="MESCA005654-PA"/>
        <property type="gene ID" value="MESCA005654"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309201_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005654-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11853" checksum="97DFBDB302DF383A" modified="2013-10-16" version="1">MHRHGFFHRDLKPENLLCSGPELVKMQISDWPEKYGRGHHLLTTFQRDGIELRKFFYIPRDTTVQLIYGQLDVLWPSCTPFGHCFLVAVKLTNFLKFVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQL6</accession>
    <name>T1GQL6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQL6"/>
    <dbReference type="STRING" id="36166.T1GQL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005926-RA">
        <property type="protein sequence ID" value="MESCA005926-PA"/>
        <property type="gene ID" value="MESCA005926"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770195_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005926-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="17132" checksum="3162DA598106E2FF" modified="2013-10-16" version="1">MQDLSDLFMLVRKSLSRYVEDVELESFDTSTSTEGEHTPTPSPQIPQTYIEYEATLQELISQQKWSQAIHHIRNYKRVVLLSSSNPNISLLSPDSDPKMTDEILTILNCYSKQTIRDRNDAFIVSKTEQELSELYIFNGFIRRLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQN6</accession>
    <name>T1GQN6_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="T1GQN6"/>
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        <property type="protein sequence ID" value="MESCA005947-PA"/>
        <property type="gene ID" value="MESCA005947"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1791701_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005947-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16933" checksum="F49E252487DAEDDC" modified="2013-10-16" version="1">MEPKVYKLLDDDEILRKIEDSGNNIEIQNIQECVEIVNKNMKDKEHFEKAGEDFKKLSPSRSLEKIENENSNFNEKHDDHIEKLSPCSSPLEKRKKFENEHEHNLKKLSPCSSPLEKSKNLERIVTSPDLFCDSDDDVEMVCNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQV6</accession>
    <name>T1GQV6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006021-RA">
        <property type="protein sequence ID" value="MESCA006021-PA"/>
        <property type="gene ID" value="MESCA006021"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185520_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006021-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8145" checksum="2316CC7270998B48" modified="2013-10-16" version="1">MWWFVGFLVVSAIAIAGRKWGRWIYLKYGRHLPLPRNLLVAKRPATPPISSINNVENNLDETREMQHTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRD0</accession>
    <name>T1GRD0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRD0"/>
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        <property type="gene ID" value="MESCA006210"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10604" checksum="176CC65E43E0D9E5" modified="2013-10-16" version="1">MQIKTHTTDVLGYCGLTPKQYTYFYTDDPFAESSPISDVVPSLMAMTTMTISIAVVVIFGTVGGSEMIFEKSDLKTLILRTTYMKDMLASVEWMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRG6</accession>
    <name>T1GRG6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRG6"/>
    <dbReference type="STRING" id="36166.T1GRG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006250-RA">
        <property type="protein sequence ID" value="MESCA006250-PA"/>
        <property type="gene ID" value="MESCA006250"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_1_1"/>
    <dbReference type="OMA" id="QCASPQY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000010">
        <property type="entry name" value="Kallikrein related peptidase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588486" description="Peptidase S1 domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="6"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006250-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="235" mass="25093" checksum="746E3745D563D5BE" modified="2013-10-16" version="1">MFRFVVLTALLAFVSAGTVPFPDGKIVGGIQLPLSNILTKCRCKSMDPMPVELPCIGLTSSVRVGSTNKTKGGQVINVKSYINHPDYSKSTHSHDVAVILLEKHAVLNTSVRTIELATTTPRTGTPAVVTGWGTEEFGVKVYPTHLIEVELKIVDAKECASSKYRYGKRINSSMVCAVAKGKDSCQGDSGGPLVADNKLVGIVSWGNGCAFEGYPGVYADVAVFHSWIVNTANSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRH9</accession>
    <name>T1GRH9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRH9"/>
    <dbReference type="STRING" id="36166.T1GRH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006264-RA">
        <property type="protein sequence ID" value="MESCA006264-PA"/>
        <property type="gene ID" value="MESCA006264"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047089_0_0_1"/>
    <dbReference type="OMA" id="HRNLPXY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000118">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF133">
        <property type="entry name" value="CADHERIN-RELATED FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="66"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="165"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="270"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="362" mass="40418" checksum="C9124D3D5CD96D4F" modified="2013-10-16" version="1">MNDNAAAKKIYNASIEGVRRRGRQRARWADLVEKDAKELRIPHGNQYLETITLTVLDKNDSPPVFRDTPLIYSVSEDLGAGHTIATIRATDPDTLGTLNYTMMNGDDGKFELESKTGKLRLQDSLDRETKDVYHLVIRVTDGMQYTDTTVTVQVNDTNDNPPVFSEAAYSFDIPENAQRGYQIGTIKATDPDVGNNALIFYSVISDWGNDVFTLNPQTGVFTLTAKLDYEEVQHYILVVQAQDNGVPSLSTTLTVYCNVLDLNDNPPIFESINYSNEIYENVEIGTEVLKVRATDADDGDNGRIEFSITAGDDRNDFEITENGTIRTKRELDRETKSSYNLIVTAKDCAKEPASKRLSSTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSD4</accession>
    <name>T1GSD4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020593">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSD4"/>
    <dbReference type="STRING" id="36166.T1GSD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006589-RA">
        <property type="protein sequence ID" value="MESCA006589-PA"/>
        <property type="gene ID" value="MESCA006589"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_922187_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000013">
        <property type="entry name" value="Histone-lysine N-methyltransferase SMYD1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053010">
        <property type="entry name" value="SET_SmydA-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455:SF3">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 9, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="24"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006589-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="291" mass="33074" checksum="8810F59CAB3F6B73" modified="2013-10-16" version="1">MICPKKLAELINTNCAPNLKQKDPNWEIGVSKIAGRGVYATRNLKCGEIIFKDDPLLIGPTLRNETPLNTCTVCYSLLDPKKFLCKQGCGLPLCENCSRKKQHKAECQLFKSWEPVEPQTPNILVIRLITVARAMLFNANQKALLYAMQANIDTTFRNEVKNASACFKNFPTDKKIVEYLHRTVAILNTNSYEVNSSKQSDFPLRALYPLAGILNHECVPNTTYTFDEKNYTIILKAAKDIKEGQEITISYTKLLWSNLMRQLFLKMTKQFSCKCQRCSDPTVSIFVPFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSF0</accession>
    <name>T1GSF0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSF0"/>
    <dbReference type="STRING" id="36166.T1GSF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006605-RA">
        <property type="protein sequence ID" value="MESCA006605-PA"/>
        <property type="gene ID" value="MESCA006605"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328363_0_0_1"/>
    <dbReference type="OMA" id="CQMSDNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="590"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006605-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="876" mass="103292" checksum="04DF6291B78A0734" modified="2013-10-16" version="1">KSIFAFANPTRFPQRKIVLNQKYVKLKRILVNENEELIYGEIDDNDDSLASLLKERQRMQEDELITPDVQIIKLKTLEVFRRNFTFQEDVEIKPDIESLQNLNSANNMIHQDELIIKCEQISIASDDDDEQEKYDIISIPSVLSDNYPEDEVFSEEDENFDYSQDFVQQQPIVDRDVHYDPLVIKSEEDFNRTKWILENAADDSDAADDSQNNPVEEIEDDEESEKEANRPYKDFFTKKRNYFVCPICGKKSKISKSHIEHLRKMHPNHPYVKRKLNVDNLKLIESKEEMQNMKTPMRKMQCVICRRKGFKGFVSYNDHLNGHRNVRSYVCACGSAFTTRHNFVSHKKQKCAHIPLEDCNIPKYAEESFQIIRKEAEPRTCQDHQSTHSDVKPYICECGFSCYFFNEISQHFQTSNCKADSAVLPEYAQRRRMNVVEVTNPPSEPEASNQEWIIERKKPKKLKIAKPKVIEEQQKTIPPAMTEDSRTRVRNKRNFPIPDRIIKDGKKVYTCWECDFASPKTTLVYNHYENRHLVRRLKCEICEKFFPSERAIKKHRRQIHEELIKCKYCDARIPRIKMKRHEELKHLRPYKCDECDSTFGYNTSLISHKMKVHEQKMLEPRFVSCIWCNATIDIRNNSFQNHMSTKSHKSYVRTWLEYNQIESIPCHLCEEWSSDNMDAVLKHLNTQHRLGSEKCMEYLSTFGCQDPALLLTLTMKERKEYVINNFEIRESLKAAPTNITCFIGGFPCVECGNQELHTLQMALQHVQNEHGSSQLEYFEYLRSEFAPFCNSGKQSHCFICSKDVFSMEQHMRNQIHRVLEKRWTRMEQLTNFPCSVCGSFFASPFETATHALETHNQPFAEYMQFLQITFLKYNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSI1</accession>
    <name>T1GSI1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSI1"/>
    <dbReference type="STRING" id="36166.T1GSI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006641-RA">
        <property type="protein sequence ID" value="MESCA006641-PA"/>
        <property type="gene ID" value="MESCA006641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="OMA" id="AKEANSW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF54">
        <property type="entry name" value="CYP9F3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="30327" checksum="F5AF0E8812D29C41" modified="2013-10-16" version="1">MKIWVEDLLDYFKRLVLDAMDYRKKNNIHRPDMINMLMEAKEANSWSDVEIVAQCFVFFLAGFETSATFLSFACHELMENSGIQKRLFEEISENYSNDELTYEDINKMTYLDAVMSETLRKWPNAAATDRQCNKTLKFENPDGLGDVVIKEGELIWIPIAGLHRDPKYFEEPKLFNPDRFSEENKRNIQPFTYIPFGVGPRACIGNRFALMMAKTILCYIIKNFELKPSPKSTIPLDLTGGGFRMKPKKGFWLNLRNRSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSM8</accession>
    <name>T1GSM8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006688-RA">
        <property type="protein sequence ID" value="MESCA006688-PA"/>
        <property type="gene ID" value="MESCA006688"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815390_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006688-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7745" checksum="332ED2A4563F113C" modified="2013-10-16" version="1">MVLHLTIYDIHEDSVSTAIALSKDKCVLDARPPLVDPSERSLQRERRTTPIRFLKHRLKLPCSNHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU17</accession>
    <name>T1GU17_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU17"/>
    <dbReference type="STRING" id="36166.T1GU17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007217-RA">
        <property type="protein sequence ID" value="MESCA007217-PA"/>
        <property type="gene ID" value="MESCA007217"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962103_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007217-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14289" checksum="4D82BF2E72A4E2D2" modified="2013-10-16" version="1">MKTKQKSEKIKFHQLFRYSTKLDKLFLIIGLVFTLFGSLGFPVYILVYGEGVNLLVERVSPASSRQTLDTPIISGFGGGNTLEPNSSKDLQMIEMKKDTLAHLYATFAIAVIDILVLGIGIYFIHISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU72</accession>
    <name>T1GU72_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183888">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU72"/>
    <dbReference type="STRING" id="36166.T1GU72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007280-RA">
        <property type="protein sequence ID" value="MESCA007280-PA"/>
        <property type="gene ID" value="MESCA007280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2596960_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8924" checksum="0FAB5B50FB0CC66D" modified="2013-10-16" version="1">HNGKNVISDLYLEENIIPDGHFLRIQSPNQGQDIIKNFTKTDLDLCHYQGKVRGSPNSKVAISTCMVVSRELSLMAPTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUK2</accession>
    <name>T1GUK2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUK2"/>
    <dbReference type="STRING" id="36166.T1GUK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007417-RA">
        <property type="protein sequence ID" value="MESCA007417-PA"/>
        <property type="gene ID" value="MESCA007417"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_951800_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007417-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="293" mass="32680" checksum="B8898A494181A640" modified="2013-10-16" version="1">QSSSEEFKKVPAIPARTTDRPPPEIPPKRHSLKKIPPEGVVPMRVPPPLPQKPSSAQNSPQLAVKLRETIPQGSPPMPPRPVSSVAKFNTPRSTPVSANNSPQLPVRKNVPTKKISPTEDFGSEDALRGIESGLRNMERAMEEQMKAAAAAAAAEEINMNFNQPLRGLERNLSMDQMMLENFAGNLRSMESNPNIRKTFEEIKQQQQQQQQGQRVQSAVPLENHMRSLDRSLPLELQYSRHRQQQQQQQQVEQQPSNNSVIPLSGDFREHIRQQLMSGGSVTVVPNASINNVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUQ0</accession>
    <name>T1GUQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Larval cuticle protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUQ0"/>
    <dbReference type="STRING" id="36166.T1GUQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007468-RA">
        <property type="protein sequence ID" value="MESCA007468-PA"/>
        <property type="gene ID" value="MESCA007468"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="OMA" id="GFQPQVC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577644" description="Larval cuticle protein 8" evidence="3">
        <location>
            <begin position="17"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007468-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23008" checksum="CC067C3017BBBCFC" modified="2013-10-16" version="1">MKLAIIFAVVLALTLAAPPNTPDTQITKLVNDVASDGFNYNLETSDGTSQKAQGELKKVDAENSAVVVKGSFSYIASDGQQYTVTYVADEKGYQPSGKHLPKPIPVNMKFIVLFVAIFALAAAAPIENDPKLAETLRYENDNIGVDGYHFAIETSDGTSRQETAQLKQIDSENVALVIQGSYQYVGNDGQTYEVKYIADENGFQPQGAHIPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUZ5</accession>
    <name>T1GUZ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoside hydrolase family 31 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02080649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUZ5"/>
    <dbReference type="STRING" id="36166.T1GUZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007568-RA">
        <property type="protein sequence ID" value="MESCA007568-PA"/>
        <property type="gene ID" value="MESCA007568"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1349874_0_0_1"/>
    <dbReference type="OMA" id="APIMEEG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF6">
        <property type="entry name" value="SITS-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="4">
        <location>
            <begin position="30"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="5">
        <location>
            <begin position="146"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007568-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26443" checksum="58EE60BBCA923155" modified="2013-10-16" version="1">MDLDAQRIFGISAAASTPKPPTFVSLPPMNSTWDSLRTLVTSAVKSGILGYPFLMSGAVGGDYHFTRNTTKMMTFYSLDSPPLPSKELYIRWMQIAIFLPSMSFVHLPLDYQDPYVTDVMKDLMNTRQKTVIPVLKKFLKESLNNGLPLIRPMWMLDPYDYNCININDEFTIGDELLVAPIMEEGSEIYLPAGVWQDGIDGTIRKGNIWLHQYKVPKEKIAYFVKMPNNTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV30</accession>
    <name>T1GV30_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104545">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104546">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV30"/>
    <dbReference type="STRING" id="36166.T1GV30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007610-RA">
        <property type="protein sequence ID" value="MESCA007610-PA"/>
        <property type="gene ID" value="MESCA007610"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_974140_0_0_1"/>
    <dbReference type="OMA" id="HHAMSGA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002540">
        <property type="entry name" value="Zinc finger protein Nv-ZicE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="254"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007610-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="286" mass="32493" checksum="B12357A0697A1673" modified="2013-10-16" version="1">MMMNAFIENQSSHHHLASYGLRMSPNHSQLQSASSESSLLQQQFEQSPGQSAMVNNDMSVYQNSYGSTMGQLGPYSRDFMHPSRSSNSSGPDTGLALFPPPIHHTHSALHESPSIHAHHQNPMRFFQSVADYPYPAMTHHQPNFHNVHPHHAMSINSTPLSRYYHSPKPGSIVKQEMTCLWIEPQTQFSAEKTCNKIFNNMQEIVAHLTQDHVGGPECTTHSCHWADCSRQGKPFKAKYKLVNHIRVHTGEKPFRCPFPNCGKDFARSENLKIHKRTHTEALQLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW60</accession>
    <name>T1GW60_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008032-RA">
        <property type="protein sequence ID" value="MESCA008032-PA"/>
        <property type="gene ID" value="MESCA008032"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624846_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008032-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8606" checksum="34CECAE321361191" modified="2013-10-16" version="1">MYRVPSETVAIQTPIVQNKSSAISSGNLASSTIRSLDLKSKNIKRTTLNPMDGELIITMKCASSIILQTWLKRSKQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWK0</accession>
    <name>T1GWK0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Collagen IV NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWK0"/>
    <dbReference type="STRING" id="36166.T1GWK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008177-RA">
        <property type="protein sequence ID" value="MESCA008177-PA"/>
        <property type="gene ID" value="MESCA008177"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847396_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF914">
        <property type="entry name" value="OTOLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008177-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15090" checksum="3FF3B5379FE031FB" modified="2013-10-16" version="1">MSHMQKFFFECNSLSSSCKWNSRRCKPGEPGIEGSKGDKGAQGPQGAKGEMGKDGSAGIPGLLGVDGEKGNKGPQGEDGYFENLDLEQELKNLLENDKQLLEYFFDLSKDLIRGEKGDKGPKGENGCEKANRNTISKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX52</accession>
    <name>T1GX52_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX52"/>
    <dbReference type="STRING" id="36166.T1GX52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008393-RA">
        <property type="protein sequence ID" value="MESCA008393-PA"/>
        <property type="gene ID" value="MESCA008393"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_10_5_1"/>
    <dbReference type="OMA" id="KLRFFFX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008393-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12752" checksum="CD149D9D73CEF1ED" modified="2013-10-16" version="1">MNPHWYGPIMLDADEIRRLGKRFRKLDLDNSGALSVDEFMSLPELQQNPLVQRVIDIFDEDGNGEVDFKEFIQGVSQFSVKGDKLSKLRFAFRIYDMDNDGYISNGELFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXG6</accession>
    <name>T1GXG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008517-RA">
        <property type="protein sequence ID" value="MESCA008517-PA"/>
        <property type="gene ID" value="MESCA008517"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375144_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008517-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10751" checksum="138EF9E569E331A3" modified="2013-10-16" version="1">MEERQDFINLDEDEAEDFDKEFQTKVRSNGRYNNDGYQMARITEVGEDYPSSSQGLRALPAPPHRRHLLLLRQLLGMGSIIPTGISKPLSSLGMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXS4</accession>
    <name>T1GXS4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXS4"/>
    <dbReference type="STRING" id="36166.T1GXS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008629-RA">
        <property type="protein sequence ID" value="MESCA008629-PA"/>
        <property type="gene ID" value="MESCA008629"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662805_0_0_1"/>
    <dbReference type="OMA" id="CIEAMRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF1008">
        <property type="entry name" value="KINESIN-LIKE PROTEIN SUBITO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008629-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18228" checksum="B68A8E0CBA8584E1" modified="2013-10-16" version="1">MLHVIKFSLPNLITHKTYRFCDLAGSERQTKTNCEGSRLKEAQQINKSLVVLGRCLDAANSNLQKKANQDVIPIRESKLTMLLQPPLLGKEKLCMIVALTPIEQFFEENMNVLNYASIAKNIVYKSQIKKDVGRRFSWFTSTNYESRGNDQLDLMEDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXU0</accession>
    <name>T1GXU0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXU0"/>
    <dbReference type="STRING" id="36166.T1GXU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008647-RA">
        <property type="protein sequence ID" value="MESCA008647-PA"/>
        <property type="gene ID" value="MESCA008647"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729579_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043195">
        <property type="entry name" value="TTC12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008647-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8786" checksum="3AA53900DA4659FE" modified="2013-10-16" version="1">LKLNKRAVIDCDFVINKIDEKNLRAWLYRAAAYFKQDDMKNFENCVKLVKKNNPKELEYIEEFVEKIRKEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY72</accession>
    <name>T1GY72_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008794-RA">
        <property type="protein sequence ID" value="MESCA008794-PA"/>
        <property type="gene ID" value="MESCA008794"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2892486_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008794-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="6773" checksum="95309EBC96E47444" modified="2013-10-16" version="1">MADTELKQSVWTEVLLPIISAKINLLKTIIETWFAKKGGSIWATILGFLVFPGLGGILGTVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ48</accession>
    <name>T1GZ48_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02098348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ48"/>
    <dbReference type="STRING" id="36166.T1GZ48"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009141-RA">
        <property type="protein sequence ID" value="MESCA009141-PA"/>
        <property type="gene ID" value="MESCA009141"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485610_0_0_1"/>
    <dbReference type="OMA" id="RSHEQKP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048619">
        <property type="term" value="P:embryonic hindgut morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016348">
        <property type="term" value="P:imaginal disc-derived leg joint morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007366">
        <property type="term" value="P:periodic partitioning by pair rule gene"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000958">
        <property type="entry name" value="Odd skipped"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002020">
        <property type="entry name" value="Odd skipped"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001452">
        <property type="entry name" value="Odd skipped, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000311">
        <property type="entry name" value="protein odd-skipped-related 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF13">
        <property type="entry name" value="PROTEIN ODD-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0562">Pair-rule protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009141-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="21141" checksum="FB3EBF0B7EABA4B0" modified="2013-10-16" version="1">MRAYPEMQRLHGGRLDGPVKIAGQRPKKQFICKFCKRQFTKSYNLLIHERTHTDERPYPCDICGKAFRRQDHLRDHRYIHSKEKPFKCTDCGKGFCQSRTLAVHKVTHLEEAPHKCPICNRTFNQRANLKAHMQQHVEEQKQQQQQQSSLELMKNQAEGMIDSEIEIDVGGADSLIGVKMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZB0</accession>
    <name>T1GZB0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin type III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009208-RA">
        <property type="protein sequence ID" value="MESCA009208-PA"/>
        <property type="gene ID" value="MESCA009208"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309206_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032073">
        <property type="entry name" value="FNDC5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21104">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21104:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16066">
        <property type="entry name" value="DUF4808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type III" evidence="1">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009208-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11496" checksum="D4138223B53A970B" modified="2013-10-16" version="1">MVLASEYEGCKDLHIKQADSSVHFHLLQEEPSTAIDQRCTRSRINSAIFVSSEGRGFDSIEFLRRHGSQSVLCRKARSAENITDDDRKFCIYNMFYSTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZM6</accession>
    <name>T1GZM6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cleavage stimulation factor 50 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02192392">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZM6"/>
    <dbReference type="STRING" id="36166.T1GZM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009330-RA">
        <property type="protein sequence ID" value="MESCA009330-PA"/>
        <property type="gene ID" value="MESCA009330"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041619_0_0_1"/>
    <dbReference type="OMA" id="NEREHLY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005848">
        <property type="term" value="C:mRNA cleavage stimulating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.50:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000064">
        <property type="entry name" value="Cleavage stimulation factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.50">
        <property type="entry name" value="Cleavage stimulation factor subunit 1, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044633">
        <property type="entry name" value="CstF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032028">
        <property type="entry name" value="CSTF1_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038184">
        <property type="entry name" value="CSTF1_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44133">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44133:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16699">
        <property type="entry name" value="CSTF1_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Cleavage stimulation factor subunit 1 dimerisation" evidence="8">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="156"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009330-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="320" mass="35563" checksum="3D5FD34EBE45E9A4" modified="2013-10-16" version="1">RNLLKNRELLYRLIISQFLYDGLEKIAKEVQMIVKADPDCAPSEQLLHFMIAGMQLEKPFEEKSLGDDASGGIDLEFEPEASGMAPEPACYETAYVTSHKQSCRSGAFSHDGQLVATGSVDASIKILDVDRMLAKSAPEEMDPGREQHGHPVIRTLYDHTDEVSHLEFHPKEYILASASRDCSVKLFDIAKPSVKKAHKIFSDCEPVTCMAFHPSGDSMAIGTEHPVLRIFDVNTSQCFVSAIASQQHKASVMCVKYSPTAKFYASGSKDGCIKLWDVVSGRCINTYQDAHSGAEICSLQFTKNIYTRVVQIWKNFPVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZZ1</accession>
    <name>T1GZZ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZZ1"/>
    <dbReference type="STRING" id="36166.T1GZZ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009453-RA">
        <property type="protein sequence ID" value="MESCA009453-PA"/>
        <property type="gene ID" value="MESCA009453"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091840_0_0_1"/>
    <dbReference type="OMA" id="NHYLTEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF54">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL-GAMMA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="9">
        <location>
            <begin position="13"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="265" mass="30578" checksum="987E7A7E2084EE7A" modified="2013-10-16" version="1">MGTWDPMLLSEGLFSAANIFSSLKLVYIFSVNPHLGPLQVSLSRMVMDIMKFFFLYVLVLFAFGSGLNQLLWYYSDLEKKRCPELFNYHNISYTLPINGTNDPNACIVWRRFSNLFETTQTLFWAVFGLIDLDSFELDGIKSFTRFWGMLMFGTYSVINIVVLLNLLIAMMNHSYQLISERADTEWKFARSKLWISYFEEGGTCPPPFNIIPTPKSIVYVFKWAKRVFCSGSSAARREHLQTIRKTIYLNDMLEIGTLGAHFCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H021</accession>
    <name>T1H021_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H021"/>
    <dbReference type="STRING" id="36166.T1H021"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009485-RA">
        <property type="protein sequence ID" value="MESCA009485-PA"/>
        <property type="gene ID" value="MESCA009485"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888329_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009485-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7160" checksum="7396F6AC79684A01" modified="2013-10-16" version="1">MNKFNSKCSVEKNETLGRFVVASDDLDEGETVLIEDPILIFPVFGDDIQRCCKCFKKTIDICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0N9</accession>
    <name>T1H0N9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02378803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0N9"/>
    <dbReference type="STRING" id="36166.T1H0N9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009721-RA">
        <property type="protein sequence ID" value="MESCA009721-PA"/>
        <property type="gene ID" value="MESCA009721"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_2_0_1"/>
    <dbReference type="OMA" id="RYVETIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000076">
        <property type="entry name" value="phospholipase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002334">
        <property type="entry name" value="Allerg_PlipaseA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF150">
        <property type="entry name" value="FI01825P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00825">
        <property type="entry name" value="DOLALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="16"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009721-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="298" mass="32316" checksum="9DC6BC6B7AFD051F" modified="2013-10-16" version="1">MSEEDAQMIGRDLAVTSRAAKIKLYLYTKQNPTVPQLIDASSVESLKNSNFKASRPTRIIIHGWQNNYLSDVNVDLRTAYMQHGDYNIICVDWSANAETINYPLAAQRVKGVGKQVAAFIDFANTNVGLSFETLEIDGHSLGAHCAGFAGKNVKNGRIHTIRGLDPALPLFSYDKPADRLASTDAYYVESIQTCGGLLGFLQPIGKAAFYPNGGKTQPGCGLDVSGACAHSRSYEYYAEAIVRNDFPSMRCSDYENAVDKNCGKTYSANKLGSPSNDNSASGSYYTPVNKKSPFGMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H130</accession>
    <name>T1H130_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02058958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H130"/>
    <dbReference type="STRING" id="36166.T1H130"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009881-RA">
        <property type="protein sequence ID" value="MESCA009881-PA"/>
        <property type="gene ID" value="MESCA009881"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560879_0_0_1"/>
    <dbReference type="OMA" id="HYSEVEX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039289">
        <property type="entry name" value="CHCHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622:SF0">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CHCH" evidence="10">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="47"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009881-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9178" checksum="06D614B99695DE08" modified="2013-10-16" version="1">MATGPCGVDFREAFSCFHYSTAEPKGSDCYEQFKTMQDCFAEYPAVYNKNDGDDDDSMPDLNSLDDENESQEAPKSDAVEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1A4</accession>
    <name>T1H1A4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1A4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009961-RA">
        <property type="protein sequence ID" value="MESCA009961-PA"/>
        <property type="gene ID" value="MESCA009961"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815348_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009961-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7907" checksum="2D410BBE6F6199BB" modified="2013-10-16" version="1">MELRAAISIYVILQFKTNVIRNRRTRNGQRVKCSNLEHQDHVETRSHKRPKESTEKYGVDITALQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1B7</accession>
    <name>T1H1B7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-binding enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1B7"/>
    <dbReference type="STRING" id="36166.T1H1B7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009976-RA">
        <property type="protein sequence ID" value="MESCA009976-PA"/>
        <property type="gene ID" value="MESCA009976"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_17_6_1"/>
    <dbReference type="OMA" id="KEHFINH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009976-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14546" checksum="FBDE007A4CA9E224" modified="2013-10-16" version="1">MTKCFEKYERVAWVTIDRIKEIMKSKGYHISPSEIEELVLEIPEVADICVVGIPDVVTINLPAALVIRKNGSTLSQEAIQKYVADKMPHYKHLTGGVYFVDSLPRTPSGKILRRKAKEIAELFFKEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1C9</accession>
    <name>T1H1C9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1C9"/>
    <dbReference type="STRING" id="36166.T1H1C9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009988-RA">
        <property type="protein sequence ID" value="MESCA009988-PA"/>
        <property type="gene ID" value="MESCA009988"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344631_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009988-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="23689" checksum="ED613399EF0149E6" modified="2013-10-16" version="1">MSASATSYSSSSSSTSGDGIINFNCDNLFVVNCLEKENNCSNRMPPKEKKAKKVIDYAADDLFDPNRSIINIEYSEECPVFHLKAVEFLNFLIERFPERKFVLIKNKDPPRPGAFEINFSQTARTSNHCLWTGVQKGPPRQRKFPKSYEDLLPEVDKILKKIYVVIKKAMKKRSKLTLKMILELKYCRKLLKKLLRNRWHSVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1H9</accession>
    <name>T1H1H9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1H9"/>
    <dbReference type="STRING" id="36166.T1H1H9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010041-RA">
        <property type="protein sequence ID" value="MESCA010041-PA"/>
        <property type="gene ID" value="MESCA010041"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_0_3_1"/>
    <dbReference type="OMA" id="CAVERIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="71"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010041-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="265" mass="29494" checksum="B4788140F3BD4361" modified="2013-10-16" version="1">IKDSSQSQTPNIRNYISKARCLNQPDPLAVCCVPQFIEGQKSPLISRKIGKSKSSSKINYLNCGETNTNKISNGNQSFPGEFPFMALLAYEGIGGTKDFKCGGSLINSRYVLTAAHCLTIPNLIAARLGENTIGKTEDCFKILNGKTVCAPPVIDYGIDEKIIHPSYNSPRRVNDIGLIRLREEVPLDNRNFVNTICLPDPEVLTNQKSRTRFLVAGWGRTENSQRSNNLLKALIPRQPIDECKSIFGLDYLEDDKTICAVERIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1K6</accession>
    <name>T1H1K6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1K6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010069-RA">
        <property type="protein sequence ID" value="MESCA010069-PA"/>
        <property type="gene ID" value="MESCA010069"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662791_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010069-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18157" checksum="8CE09CBFA9FA0447" modified="2013-10-16" version="1">MVQHLGTPWLRADVSLSPNPSSIMGISRRSHYEPNRSGKNNGHRDSCNAESSNTFGLETRKKRPKKNESNEGSASKQKEGGCERGYTKEKEIRESPVWNQARKFYENVNIERKGIRRRSLCNGKFSLKNYRTATKVERPLKEDSPRSSKTMKMKPTRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2M3</accession>
    <name>T1H2M3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2M3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010473-RA">
        <property type="protein sequence ID" value="MESCA010473-PA"/>
        <property type="gene ID" value="MESCA010473"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3377604_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010473-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="34" mass="4124" checksum="9E878B3EDF1515E1" modified="2013-10-16" version="1">MLKYYRKYHQQHRHLMLSQNARKAISAVVPQTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2N1</accession>
    <name>T1H2N1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytosolic Fe-S cluster assembly factor NUBP1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer of 2 NUBP1 and 2 NUBP2 chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2N1"/>
    <dbReference type="STRING" id="36166.T1H2N1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010483-RA">
        <property type="protein sequence ID" value="MESCA010483-PA"/>
        <property type="gene ID" value="MESCA010483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="OMA" id="VSGCPMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001759">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="85"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="332" mass="36042" checksum="96890D56E6A22EB4" modified="2013-10-16" version="1">MIFKPQLSSHIYYQSNEIHRIKNNGSRTRKCPGVGSESAGFSSACAGCPNQQICSDPNKPTVDPGKALVDERLKTVKNKLLILSGKGGVGKSTVTMLLTRFLAKKFPDKNFGVLDLDICGPSQPRILGVENEQVHMSGSGWSPVSVDDNICLMSIGFLLSSNDDAIIWRGPKKNGMIRQFLSEVDWGNLDLLLLDTPPGTSDEHLSATSYLKSENEKVKALIVTTPPEVSIIDVRKEISFCKKQGIEIVGVVENMSEFVCEHCNKSSEIFPAKSGGAATMCEDLGVKFLGSLPLDLNIAKACDKGEDITQFKGKTVEALEKICNSILTSCEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H405</accession>
    <name>T1H405_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Deoxyribonuclease TATDN1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Deoxyribonuclease which catalyzes (in vitro) the decatenation of kinetoplast DNA, which are circular DNA catenated to each other, producing linear DNA molecules. Plays an important role in chromosomal segregation and cell cycle progression during eye development probably via its DNA decatenation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H405"/>
    <dbReference type="STRING" id="36166.T1H405"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011002-RA">
        <property type="protein sequence ID" value="MESCA011002-PA"/>
        <property type="gene ID" value="MESCA011002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_1_2_1"/>
    <dbReference type="OMA" id="HDAKSWE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="298" mass="33551" checksum="562C252B89AC280C" modified="2013-10-16" version="1">MAICFDFMRIVSGILPAYNWYTIVMTSINSLKEGSAIEIENLIVIDAGANLTNRKYARDLELVIKRATESGVRKIMVPGNSVRSSKEALRLSRIYPDIIYSTAGIHPHDSKSVIDVTDSWNEFEEIARSPECVAIGPCGLDVRTEITNYDIQKQVFEKQILLACSIKKPIFIQERSAQSDILEILGKYSNLPSVIIRGFSGTSEEAIKYINNNCFISLNGFLCKDKSDIGIRKLLEDCTIPLQKLIVESDAPFMFPNTRASKLPQYVKSALSERSLIYLNRYCTFQRNEPCCLPSIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4G9</accession>
    <name>T1H4G9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4G9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011178-RA">
        <property type="protein sequence ID" value="MESCA011178-PA"/>
        <property type="gene ID" value="MESCA011178"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515228_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9443" checksum="52A5C9DE5CB6E8E7" modified="2013-10-16" version="1">MVHLGGRLDITINCISSTNIQDHSKTSSMGKSCAKNGVKFTNGLSACTLLLRFVATNLQADFAWLTKFLKALDFELFFSNWKVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4W5</accession>
    <name>T1H4W5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02136570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4W5"/>
    <dbReference type="STRING" id="36166.T1H4W5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011336-RA">
        <property type="protein sequence ID" value="MESCA011336-PA"/>
        <property type="gene ID" value="MESCA011336"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177363_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005289">
        <property type="term" value="F:high-affinity L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF1">
        <property type="entry name" value="SD08189P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011336-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12540" checksum="24617608F2FCE7F2" modified="2013-10-16" version="1">MDIIMGIHWTQSRHGNRPQIQRCRTQWTKSSKGIKGGMLFPLLTTGGINSILFGVYGNHLRQLQRTCHSDLQRKQLEDNHIFAAGTLAGFVQSFIACPFEIVKIRLQTHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H525</accession>
    <name>T1H525_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vesicle tethering protein Uso1/P115-like head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02383413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H525"/>
    <dbReference type="STRING" id="36166.T1H525"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011399-RA">
        <property type="protein sequence ID" value="MESCA011399-PA"/>
        <property type="gene ID" value="MESCA011399"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1708734_0_0_1"/>
    <dbReference type="OMA" id="IGICIFY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045056">
        <property type="term" value="P:transcytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006953">
        <property type="entry name" value="Vesicle_Uso1_P115_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04869">
        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="3">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011399-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17340" checksum="01BF7AA44E290B28" modified="2013-10-16" version="1">EISSNEQDENEYLIQGLCAFLLGLCIQCNDNTVMGNGKEDLCQLIEKRIGLEIYSRKLGEVSRHESYSRAGKHPQIRVNLGSDLLLDFEFCKLFKTLEHTISKLINGYSGNNTNLAELTLSSEASDLVGQYKGIIRDLDQEIKSLKEQVKDVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H580</accession>
    <name>T1H580_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Wiskott-Aldrich syndrome protein family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H580"/>
    <dbReference type="STRING" id="36166.T1H580"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011455-RA">
        <property type="protein sequence ID" value="MESCA011455-PA"/>
        <property type="gene ID" value="MESCA011455"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1437840_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21369" checksum="4E9B5FDE8D2546B4" modified="2013-10-16" version="1">MNQKEHARLQLRYSLEYCYYHQFDELCVDLPLHMRTEVSTWYLITTSNDVNGSGITESGYYNNSSSMCVAAVTAATNSTSSGCTNNNNSKARVRDEEHTTKMMMTTTMKMKNNHAFNSMITGFELESMCNITLSNALRQLGSLLVIANNMFSVLNCELQGIADRTNKIKQKIQLLEENVYSFDPKLVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5K4</accession>
    <name>T1H5K4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5K4"/>
    <dbReference type="STRING" id="36166.T1H5K4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011585-RA">
        <property type="protein sequence ID" value="MESCA011585-PA"/>
        <property type="gene ID" value="MESCA011585"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326657_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011585-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="10889" checksum="0FA7D5C294D374D4" modified="2013-10-16" version="1">MVVANIRSSGNVVESFMRLERCRIRGSSSLVVKYFVYLGSEVTSNNDYVVSSPNQGQVMEGMFVPWCYNCGANDLLQAVGIVGAVIMPHNLYLHSALVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5R4</accession>
    <name>T1H5R4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flotillin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5R4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012047-RA">
        <property type="protein sequence ID" value="MESCA012047-PA"/>
        <property type="gene ID" value="MESCA012047"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1316746_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045661">
        <property type="term" value="P:regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF46">
        <property type="entry name" value="FLOTILLIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="FLOTILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012047-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="22778" checksum="7B9185620B9A07A9" modified="2013-10-16" version="1">MGIEILSFTIKDVYDDVQYLASLGKAQTAMVKRDADAGVAEANRDAGIREAECEKSAMDVKYSTDTKIEDNSRLFKLQKANFDQEINTAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKTDEIVLISGSDHITGDVTRLVGQLPPAINALTGVDLSKVLSKIPGAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5U1</accession>
    <name>T1H5U1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase II subunit beta</fullName>
            <shortName evidence="2">CK II beta</shortName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5U1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012074-RA">
        <property type="protein sequence ID" value="MESCA012074-PA"/>
        <property type="gene ID" value="MESCA012074"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034027_3_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012074-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="24801" checksum="FD1CED0A697F84A6" modified="2013-10-16" version="1">KMSSSEEVSWVTWFCGLRGNEFFCEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPEDELDENPHQSDMTEQAAEMLYGLIHARYILTNRGIAQMIEKYQTGDFGHCPRVYCESQPMLPLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLYGFKIHSLAYQIQLQAAANFKMPLRSVNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H616</accession>
    <name>T1H616_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H616"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012149-RA">
        <property type="protein sequence ID" value="MESCA012149-PA"/>
        <property type="gene ID" value="MESCA012149"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2549521_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012149-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9183" checksum="AC8354826F4F083F" modified="2013-10-16" version="1">WEWKLPFNPNFQDVTGQTALYISCILGNATLKLMSSFNLSSMAAKEEDLXKCPISINLLCGAVQETALLAAVRGGFYFVTPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H620</accession>
    <name>T1H620_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H620"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012153-RA">
        <property type="protein sequence ID" value="MESCA012153-PA"/>
        <property type="gene ID" value="MESCA012153"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191691_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012153-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="44" mass="4761" checksum="14E89152E7CE3996" modified="2013-10-16" version="1">GMGGGGGHGSQNNPWMKLMGIVHKDRRITSDSHNKDGSNERNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Z6</accession>
    <name>T1H6Z6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6Z6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012478-RA">
        <property type="protein sequence ID" value="MESCA012478-PA"/>
        <property type="gene ID" value="MESCA012478"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2765152_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012478-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7785" checksum="404CC8792E6BC725" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGISETAMIFAGVDVTREPIPVLPTVHYNMGVFQPTTEDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H767</accession>
    <name>T1H767_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H767"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012605-RA">
        <property type="protein sequence ID" value="MESCA012605-PA"/>
        <property type="gene ID" value="MESCA012605"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070409_1_0_1"/>
    <dbReference type="OMA" id="TGYEFPL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="79"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012605-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="22502" checksum="D38D1F20F20E4FFB" modified="2013-10-16" version="1">KEKKDDASSDEERWLDAIESGKLEEVDDELKKIKDPKLMTARQRAMYDRNHDKDAINAPDMLMSLPTGYREKEKVMTAEALQKAQLQSARRKQQMDEKREKDKKKTMERLLKKQDSKQRGCTQIKAKLLKPKISYKNFSDNCIEMPLGFDFPLKHLCKNPEXVCGIKGCKNKIYNCSKTNIPLCSTACYKKNXI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H770</accession>
    <name>T1H770_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H770"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012608-RA">
        <property type="protein sequence ID" value="MESCA012608-PA"/>
        <property type="gene ID" value="MESCA012608"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253377_0_0_1"/>
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    </dbReference>
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        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012608-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="221" mass="25294" checksum="560C3D641F355E58" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEALVERFAYIHDKMKLSHEMIINFPQILTSRQFRIEQRHKFLDYIGKAQYDPKKDLYISPKDLVEGSDFDFVTKVAKSDMVTFDAFLRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA29</accession>
    <name>T1GA29_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000074-RA">
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        <property type="gene ID" value="MESCA000074"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576582_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000074-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9218" checksum="EC76320C6D4A8E61" modified="2013-10-16" version="1">MEPLVIGAVNHSSVQKWLPCHFTIPEITTFAKRHILHITMGKKQVSISFGVTPLSFSRAQITYRPIDGWTEVMSIYMMIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA35</accession>
    <name>T1GA35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000080-RA">
        <property type="protein sequence ID" value="MESCA000080-PA"/>
        <property type="gene ID" value="MESCA000080"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212945_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000080-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12914" checksum="BF36846C00311DFD" modified="2013-10-16" version="1">MISLGAEPAAIEDHITFCRRPYKTDIGYTEKASFCNKNRIDRDFREQTSQMISKFSLTYFTFQYPNRLRLYKTQHSVYQEIFIPRDPVEQGKRSQQQQPYFVNFHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA88</accession>
    <name>T1GA88_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA88"/>
    <dbReference type="STRING" id="36166.T1GA88"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000138-RA">
        <property type="protein sequence ID" value="MESCA000138-PA"/>
        <property type="gene ID" value="MESCA000138"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1422999_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000138-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="22293" checksum="345DC1B1EAA1C99C" modified="2013-10-16" version="1">MNKELDHIGEVTNAGDLKEKLQEAEDAKTSVEAILLERNSLLQETCEEWDQCEKKVKDIRAWIEKTETLWTPHNTKRNLYVHFRNGIGGDPRISENVDDLLKVLDELNDLVKTKTSSLEETLQQIDVYQQQMQTLRQKIIQEEQQLRLVIAPTYLPHDRERALAEQQITSRNERIKLLIHRGTPDDTILDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBA4</accession>
    <name>T1GBA4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable U3 small nucleolar RNA-associated protein 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBA4"/>
    <dbReference type="STRING" id="36166.T1GBA4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000531-RA">
        <property type="protein sequence ID" value="MESCA000531-PA"/>
        <property type="gene ID" value="MESCA000531"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778763_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000531-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="8222" checksum="2B1335636131D36F" modified="2013-10-16" version="1">MSSWKKAGKSNQKTHRERQQPESRKHLGLLEKKKDYKLRAIDRAEKEKTLKVLHKRALNKNPDDSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBE3</accession>
    <name>T1GBE3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000575-RA">
        <property type="protein sequence ID" value="MESCA000575-PA"/>
        <property type="gene ID" value="MESCA000575"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1262830_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000575-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="24476" checksum="8B3CA7B9FBF206DF" modified="2013-10-16" version="1">MGLGEIVDPIFFRILKLSSITMNWKYICLVFLITLSSTFARPSDIATSSDQPLEASVAEKQSSIRSALLSRSKIKTKPPDDDDDQESRYGSTPYADRMSYYEYHKPPAAEVNIASNSGTHYYSHHYPSAAVAYQQKPSFVAISPSQVNIPTYYTQLDTTTTTSPRPPLPPPFSTRPMIEIPRPIKVQSQIQQKPVPKPTLLISSLTQLERITQLPIPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBE6</accession>
    <name>T1GBE6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate dehydrogenase assembly factor 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDHAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02039164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000578-RA">
        <property type="protein sequence ID" value="MESCA000578-PA"/>
        <property type="gene ID" value="MESCA000578"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160299_0_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012875">
        <property type="entry name" value="SDHF4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28524">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28524:SF3">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07896">
        <property type="entry name" value="DUF1674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="36"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000578-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8970" checksum="442494A7F2236B0B" modified="2013-10-16" version="1">MSAQPIYRIATKPSILKQAKEARVLFGLQDKFFNKSKKPSDPFKPFPNGVNPKTGEVGGPTGPEPTRYGDWERRGRCSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBS4</accession>
    <name>T1GBS4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBS4"/>
    <dbReference type="STRING" id="36166.T1GBS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000712-RA">
        <property type="protein sequence ID" value="MESCA000712-PA"/>
        <property type="gene ID" value="MESCA000712"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097311_0_0_1"/>
    <dbReference type="OMA" id="IYLIDSH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF44">
        <property type="entry name" value="LDL RECEPTOR RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="LDL-receptor class B" evidence="3">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000712-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27924" checksum="1A87258639D77B1B" modified="2013-10-16" version="1">MIVRGQQVPSPARLALFENRIVWTDATKQGIMSVDKFDGPSSIEVIYKAKDIRDPKAIVAIHPLAQPKVSNPCGATNGGCNHMCIVTAVKGAGLGYRCACHTGYQLQSDLKSCRLVKEFLMYSQQRFIKGKVLEPIIEGFSDAILPVVSRRARFVGLDFDSRDEYIYYSDVLQDVIYRVHRNGTGREIVLASQNEGVEGLAVDWASKNLYYIDSRKGTLNVLSTRNVTNRRTLLKNLKRPRAIVVHPNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBW8</accession>
    <name>T1GBW8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bridge-like lipid transfer protein family member 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBW8"/>
    <dbReference type="STRING" id="36166.T1GBW8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000757-RA">
        <property type="protein sequence ID" value="MESCA000757-PA"/>
        <property type="gene ID" value="MESCA000757"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012746_0_0_1"/>
    <dbReference type="OMA" id="MHIIKGS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033616">
        <property type="entry name" value="BLTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047104">
        <property type="entry name" value="BLTP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31640:SF1">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31640">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN KIAA1109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20413">
        <property type="entry name" value="BLTP1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bridge-like lipid transfer protein family member 1 N-terminal" evidence="2">
        <location>
            <begin position="59"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Bridge-like lipid transfer protein family member 1 N-terminal" evidence="2">
        <location>
            <begin position="373"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="879"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="918"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="881"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000757-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="972" mass="110156" checksum="31EB90F812722D6D" modified="2013-10-16" version="1">MWLMISLIITLAWVVYINFYNARIFGMIVTKIINRWYITGAHFRLGSLSITPIAGKIISELYDKLEKTFGLSPSIFIPTDLSAEEKAKMKENEMNLENKRQNAFEKHKNSEAMNATTWRDLIPVIKIDICSGRFVFGNRLTPTTLSISMDEAHCTYSTKPAACQLDKFMHFVKAKVENSKVMFAPSPKYTGLADEPPRYMGEGFVVMMSNFMDLYFYMDEPGVVPDEPVHLTLANGDIIEPPPPQWGIEVKCGKGTDFSYGPWADRQRDHLFKFFFPSDWEEMQVDENPKAGQLRQYQTFEVTLCTFKEATIDILFSKDKETNAMHVTIGPGSSVEVSLPWVTHADGYQTKISGQLLHVEATTSLQYRSLADVLLKEFELLTLSNEYNWIDCSSTNQENNHLAFCGDLLDLSFVLPFDDFLPQTVELKFWIHGEGLDLSMYIPEVSSTRSIVLAIDDNAKIMTRGGEIKNRPELYSKKWRKICQRSSGWVDCWSVNIIALSITYVYHPCPPLGPDPQADITTPEKEEILLSPMRMPSKKRLSPNVNWQHNESYKFDPSSLPADKVTVDLEIGSSVMLAYGTVLRNFINLKENIFGEDQLFTDMEQSNVKMKDPGNSIQRNTCPIKKRTPYSNQPLEVIVSVMIHDIQAHLMKNCNENDPPCPVILIERLGFEMNKRYTETELQVLVSPSYLITSDTLVRPNRDKHINQGHLLLSAVQVRGHAMFSNEGCALDEDTLEYAWLLEIQLGKLSGKLTLPQLFNICVGLETFILLTMDPENCLKSPKMIRNCHHGVPTNQCPQTNEENKYKCPSCEDIKYTMTRVSIDAIDIYLVESGTALHTWISPIRLSTCNLHGQRVKSGISGLLPSVLLRLFMVNNGGSGNLTNSHSNTNTTGSNRSGRARKDGMSTYRSDLEIHAPDSKALPKRPQSSTELSDDVRRIHGLATPLRKITIPPRPEFRHYVHDVSRIDESIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCC1</accession>
    <name>T1GCC1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02156418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000931-RA">
        <property type="protein sequence ID" value="MESCA000931-PA"/>
        <property type="gene ID" value="MESCA000931"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052291_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090482">
        <property type="term" value="F:vitamin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002666">
        <property type="entry name" value="Folate_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686">
        <property type="entry name" value="FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686:SF18">
        <property type="entry name" value="IP11787P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01770">
        <property type="entry name" value="Folate_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000931-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13434" checksum="C54F2DE5474B0EFA" modified="2013-10-16" version="1">MWEDINPNPSVAWNAASDALLTLFGAIFALVAGYYNAARYHKYLSFLLLSVVALVEAGAVFMISWTTNLYVSYVGYVVFGGVYGFAVTLTSSEVMRHLVKDSYGLVLESTLWLDFYYKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCD3</accession>
    <name>T1GCD3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000943-RA">
        <property type="protein sequence ID" value="MESCA000943-PA"/>
        <property type="gene ID" value="MESCA000943"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3126720_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000943-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="50" mass="5980" checksum="2406C309428A5E4A" modified="2013-10-16" version="1">MCVPYSRTLSVSRKLCSCPEVQRQLFLRVSPPERIMLCSEKWNYKVFTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCD8</accession>
    <name>T1GCD8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02012468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCD8"/>
    <dbReference type="STRING" id="36166.T1GCD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000948-RA">
        <property type="protein sequence ID" value="MESCA000948-PA"/>
        <property type="gene ID" value="MESCA000948"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2612872_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000948-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8860" checksum="BB464D28234473EF" modified="2013-10-16" version="1">MQFHNQYLLNDHDHDVVPDDETHWQDRVSSGFDRLVAFASTELDKTRRSMDGDTIIPPSASCNTSPDSGIVQSNNSENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCG9</accession>
    <name>T1GCG9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000983-RA">
        <property type="protein sequence ID" value="MESCA000983-PA"/>
        <property type="gene ID" value="MESCA000983"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1350275_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013402401" evidence="2">
        <location>
            <begin position="16"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000983-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="22459" checksum="4ABC6E07ADD449F8" modified="2013-10-16" version="1">MIVVLVLFFRSICLYFQELVLKVHDKWLNPQHGRLYINPNDLPNSRDKVGACRFCANSPLGYCRHHNPNHTTTKRGRQNSCTSSSATSLSSSTSSHSIVSNSSGNTISSSSSSSSTTTKITNFCKCISCTSTYTSGGSSRRRKTLSTSSSDSIWKSICRAFKLGGKKEDDDYNYYHSKAPRGLEGPEIGLTYKRLHSEAQKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCI4</accession>
    <name>T1GCI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCI4"/>
    <dbReference type="STRING" id="36166.T1GCI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000998-RA">
        <property type="protein sequence ID" value="MESCA000998-PA"/>
        <property type="gene ID" value="MESCA000998"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035455_0_0_1"/>
    <dbReference type="OMA" id="DVFKGPY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577265" description="LRRCT domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000998-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="52876" checksum="A73FDFEB147601D5" modified="2013-10-16" version="1">MRISWSVFILLFAVIYTVNGEENKTTEAPKTTAVPKHPAVKTLQSALCKKCKCDKYKLLLDCSRLQLTEWFKTDEIDTLVYGNIKFETIDLSFNNLTKVPAFPKLSVKNVLLNFNKIEEIANAAFQNLTHLTKLDLSNNQLNNSKLVPEIFEGNYSPSEYEPLEFLRELNLANNDLHKLREDIFEHCDNLEFLDLSYMQLSKLPGEILHGSGNLETVVLTGNLFKLIPDEALSNTVKLKSLNMDENPIDEVGGDNVFPKLEKLEYLSLSYMNDLKTIGVGAFKNLPNLKTLQVTHNPALTTFSVEAFASYDKKNSSIVTYPTLENLYLDGNNLKKLDEKILNKWESIQQISLSNNPWDCDDHNTWFISNLVPYIHNSLSVPMARNIKCASPTEMRDKEVYEIHVQNREAELQQGGGGGSVVLGIFIGIIVGVPMTFGILLILRKYRNNSSSSTVNYNRAKFESNFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCL2</accession>
    <name>T1GCL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02083589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCL2"/>
    <dbReference type="STRING" id="36166.T1GCL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001028-RA">
        <property type="protein sequence ID" value="MESCA001028-PA"/>
        <property type="gene ID" value="MESCA001028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444388_0_0_1"/>
    <dbReference type="OMA" id="GFACIST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20455" checksum="3E4A1B2490B46CF5" modified="2013-10-16" version="1">SPRWLLANGKIDEVEKLLVHGAERNHRSTKTIRSDLDEHMSRKALLSETDLKEKAHGTLIDLFKYPNLRIRTLVMGFNWLVCGLTYFGVSQYIGEISGNIFVNVAISGMIGIPGTLISIPATKVLGRKKALILSNCVAGISLLLIAVLGKKGGWVQVGLASIGVFGMSVSFPNVYLYGGELFPTVARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCR2</accession>
    <name>T1GCR2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001082-RA">
        <property type="protein sequence ID" value="MESCA001082-PA"/>
        <property type="gene ID" value="MESCA001082"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362115_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11025" checksum="C5B37E9B275B7F16" modified="2013-10-16" version="1">MLVEAFPNPETYQEAMNQSPKTILNTDNQGTLQLTKNSSFHSGIILMNYVGTNHMTAYIFTKALPKHKIEVNYYFIRRQGFRGSFVFDNINVMKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCW8</accession>
    <name>T1GCW8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 8 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096506">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCW8"/>
    <dbReference type="STRING" id="36166.T1GCW8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001141-RA">
        <property type="protein sequence ID" value="MESCA001141-PA"/>
        <property type="gene ID" value="MESCA001141"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_289720_0_0_1"/>
    <dbReference type="OMA" id="RCLISYK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="2">
        <location>
            <begin position="479"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001141-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1023" mass="117028" checksum="31E696D9F7D4550F" modified="2013-10-16" version="1">MYYSCSLVMSTTNTTDFGKQNDASDMQTKKNTQISIRKRHGQRDQNIKLERQNPEKRRILQDCVLDITLILRNLKTHISAIPRNIRLEWALSTVSSLAFQDTPIQTSGNRLLAGFSRGLIVMFDSKTGTVLRNLFDCVTPNSGVLNLKWTEKPALALASDSGGSVWSLNFTRKFGKRGCVSKCLFSGAKGEVCAIEPLIIEDNSANNHNVTNLEQYSIIALATLSKYFVITVRPRLKVIKFHMLNGPPDSLPLLSWQMVLIQSMDSHKSVYPVLAVARGNHLCFHQIFVSQGRINLLFLRHINLNYDLLALHWLGPRVIGCVDKSEVLHLITVSSSKEEELLDLGSTGLVYGSSQFKGLATGGNVSPALSLAGNFACYNSIISSRFQHIYVLGARSVQMITIRNWNERISFLMKNKRWTEAFVLALEEFKIARKRENQRKESKEILQIVIKCLIEVNDLGSLWEYIDIGSSLSRSDLNLFLMIIAEFIEKDYITKVSPSISKLLVEYWLDLDVDKLEEMLLKLEWTCLDLNQVFRIVKDEKLYRAQIHLNANALGDYSTILMELIPKIGENGSTDRTLGNYILVYISCCLAGRAYPVGEIAMDKVSSVKHDVLRSLTSAHSILNADGELQYPYLRGLLEFDTRETLNVLALAFQEKEFQDEMGNLHKKRIVRILLEIMSGDRVHFLVKEEMYESIFDCYLPNPLRHDELFTYIENNAALPKMKVFEQIESNLLQLLEINASQIVRLIVDNYYEKFDEFLEILNENRQFLYLFMKISLSLGITLNSQMSEKYLRLLGEFEPFAILPFLETTDNYKIENAIEILKSLADFPGKTQCTIFLLEKQGDYQQAFDLSIKQLKEFDDVEGSLKVAAFCSRAVKALDEDGTEFWFRFLEIILSRGEYKVIKKRILHEAKKYVNLVKLVQLCWKIIENDLHEKFDDLKKKSNRGILVTVVRCVECRQKLYSPDSDIVVIGKCGHAVHEKCAETFKCTFCNNTSAEEGLEEVDLSKPNVELFDKQHFLNYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD26</accession>
    <name>T1GD26_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD26"/>
    <dbReference type="STRING" id="36166.T1GD26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001205-RA">
        <property type="protein sequence ID" value="MESCA001205-PA"/>
        <property type="gene ID" value="MESCA001205"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1462910_0_0_1"/>
    <dbReference type="OMA" id="HIACKND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000304">
        <property type="term" value="P:positive regulation of ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047148">
        <property type="entry name" value="PLPL9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24139:SF34">
        <property type="entry name" value="85_88 KDA CALCIUM-INDEPENDENT PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24139">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588198" evidence="3">
        <location>
            <begin position="17"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="20372" checksum="0081F7BF15201329" modified="2013-10-16" version="1">MYLLMLFLRLEHASECVSPQVAVKCGNIEMVKVLLPICKIDHLDNDGNSAFHYAAATTKDIIKLISSKSTANLNHCNVDGYTPLHLACLQDKPECVKSLLFAGADVNLEAKNVSQKTYRSAAPSSAAEFLKTNANKLYIQDMKYGGTPLHWCSSRETLQALIERGCDVNAKNFDGRTALHVMVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDG4</accession>
    <name>T1GDG4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02124319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDG4"/>
    <dbReference type="STRING" id="36166.T1GDG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001350-RA">
        <property type="protein sequence ID" value="MESCA001350-PA"/>
        <property type="gene ID" value="MESCA001350"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_4_1"/>
    <dbReference type="OMA" id="RDHPWRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12173">
        <property type="entry name" value="PGDH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000021">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938:SF22">
        <property type="entry name" value="D-3-PHOSPHOGLYCERATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938">
        <property type="entry name" value="FORMATE DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="4">
        <location>
            <begin position="36"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001350-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="27277" checksum="69551292C7BB2FF4" modified="2013-10-16" version="1">MLKTQFKEEIINIKKGQMITPGGNSISACELTCLLIGSLARPIVPAGQSMKEGKWDRKLYSGSELYGKTLAILGLGRIGREVGIRMKAWGMRLIGFDPITTEEEAKAAGIEKMSLEEIWPLADYITVHTPLIPATRNLISTETLGNCKKGVKVVNVARGGIIDEAAVLAALESGQCGGAAFDVFPEEPPKSEVTKALIAHPKVVATPHLGASTSEAQVRVAVEVAEQFIALTGKSKDYTSYLGVINKDVLKHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFA9</accession>
    <name>T1GFA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002046-RA">
        <property type="protein sequence ID" value="MESCA002046-PA"/>
        <property type="gene ID" value="MESCA002046"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429579_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002046-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10718" checksum="E7E7A53ED502D12A" modified="2013-10-16" version="1">MSLVEMWNEAFHYNDLEINMRLVEYLKRKAKEAAEEQIISFANKVANMVTFLQSCGSEHHLQNSMFLEELIGKIPSSKKFEWAHYSYSLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGD5</accession>
    <name>T1GGD5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 PI3K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGD5"/>
    <dbReference type="STRING" id="36166.T1GGD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002451-RA">
        <property type="protein sequence ID" value="MESCA002451-PA"/>
        <property type="gene ID" value="MESCA002451"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121122_0_0_1"/>
    <dbReference type="OMA" id="WRRNIEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002451-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="22447" checksum="68ED781114C99515" modified="2013-10-16" version="1">MDYESFRYVYSSSLKEKLQIKIGTLEGENQKPPDYKKLLDQSFLRYSGLYSESCPSFQVKLELFNKGKSICLPVTTSYKAFTKRWSWNEWIQFNLNFCDIPKTAVLALTIYDCTGPGKTAVIGGTSISLFGKNGLFRQGMFDLRIWLETEGDGNYPTKTPGKGKNSSKFKCKDWENWRRNIEMVKYKRLTGWTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGF0</accession>
    <name>T1GGF0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02006538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006540">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGF0"/>
    <dbReference type="STRING" id="36166.T1GGF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002469-RA">
        <property type="protein sequence ID" value="MESCA002469-PA"/>
        <property type="gene ID" value="MESCA002469"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049129_0_1_1"/>
    <dbReference type="OMA" id="LKTWCFV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000394">
        <property type="entry name" value="Gdap1, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44188">
        <property type="entry name" value="GDAP1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44188:SF1">
        <property type="entry name" value="GDAP1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="29"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="187"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002469-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39565" checksum="37CC629610E516B7" modified="2013-10-16" version="1">MSVEEKHLTNGDGGIPVIKDDFKKPELGDDLILYFHPYNFYSQKILLILHERNIKFTPYIVDLAAGEQFSSWFLNLNPKGDVPVLQDGCFIVPDSDHIINYLDGKFRSEKHPSLKSKNMQKFDLEKFNNYNQLVRKLPIGAISLGSFVHEDLKLNPKAPFIGPVRKTCLKNNEKIHDVLKNTIETQSSEEKTHQLKSTQIALKKKFEVQSKRKEIISDREKYQTLLDAVKEVLVFFDKELESTNQEWLISNNFSYADINLGLLLYRLYTLGFENYFWTYGKLPNLESYFLRFKNRESLNKTLPTSMEILKEIWAKTPSNYKISAGAGFLGMAMFAALVISRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGI7</accession>
    <name>T1GGI7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL42</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGI7"/>
    <dbReference type="STRING" id="36166.T1GGI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002507-RA">
        <property type="protein sequence ID" value="MESCA002507-PA"/>
        <property type="gene ID" value="MESCA002507"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142926_2_0_1"/>
    <dbReference type="OMA" id="MASGHLC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019346">
        <property type="entry name" value="Ribosomal_mL42"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13450:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13450">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10210">
        <property type="entry name" value="MRP-S32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002507-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="16733" checksum="A612531D6FAE5EED" modified="2013-10-16" version="1">MLKFGSRLFSTNSRSILCSKNAVGGNNLVEGVAVTSDGRTIVAWHPNKPFPYEFSKPIPTSFDDINCGSLIKETALTSAQKAFKDQRPEIARQELMNLTYTTKHRWFPRSRDKRAKDTPMDRPYLATISPDLVSLARSVKEKKVIKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGP9</accession>
    <name>T1GGP9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGP9"/>
    <dbReference type="STRING" id="36166.T1GGP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002573-RA">
        <property type="protein sequence ID" value="MESCA002573-PA"/>
        <property type="gene ID" value="MESCA002573"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017862_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF5">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE E14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002573-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13973" checksum="BC67B78365AE71F4" modified="2013-10-16" version="1">MGKFFKIRPLYLEYQPILYFSPSSPQCMTVLMLIKHINITIDLRVIDLFQGELFSAQCSVPSLIDGDATVKGSDAISIYLIEKYAKEDNLYPMNQDKLSLIHSQLQFLNVHLTLILCSSTILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGT9</accession>
    <name>T1GGT9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002620-RA">
        <property type="protein sequence ID" value="MESCA002620-PA"/>
        <property type="gene ID" value="MESCA002620"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392233_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002620-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10713" checksum="431E3A61F6F573D0" modified="2013-10-16" version="1">MNGKYNKIEDSFASGTFYYKQKPQMLLEDRSYNRLHNEKSMESIVMQTMHSSPHHVVHPTRSHPHAMKAFQVTHGSSNLLNLSGSPLLSTKSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH95</accession>
    <name>T1GH95_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="EMBL" id="CAQQ02038596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH95"/>
    <dbReference type="STRING" id="36166.T1GH95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002785-RA">
        <property type="protein sequence ID" value="MESCA002785-PA"/>
        <property type="gene ID" value="MESCA002785"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066092_0_0_1"/>
    <dbReference type="OMA" id="CINCEND"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="12"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="111"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002785-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="359" mass="42379" checksum="B5C30006BB60AF0C" modified="2013-10-16" version="1">MYKGVSKDQRDKVQLMATEKKLRTEIEDLRQQMKKVMESKREERKKLADEEALRKIKQLEEQKYELQKQVTNIKPEMNWGNAPPAGYVRPFVGSHEEEALLAEMEVTGQAFEDMQEQNSRLIQQLREKDDANFKLMSERIKANQMHKFLREEKQMLEDQSSTRDTQIEAMHIVLRKLEEKERSLQNYVSTIEKELALRQQAMEMHKRKAIESAQSAADLKLHLEKYHAQMKEAQQVVAEKTSALEAEAYKTKRLQEELAQHKRKAERMKKIEMTGVSIDEVMIEEIREYKETLTCPSCKVKRKDAVLSKCFHVFCYDCLRTRYETRQRKCPKCNCAFGAKTIIVFISLKYQLLKLLHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHI7</accession>
    <name>T1GHI7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the unc-13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02114736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHI7"/>
    <dbReference type="STRING" id="36166.T1GHI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002889-RA">
        <property type="protein sequence ID" value="MESCA002889-PA"/>
        <property type="gene ID" value="MESCA002889"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003295_2_1_1"/>
    <dbReference type="OMA" id="TYYHPIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04009">
        <property type="entry name" value="C2B_Munc13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052095">
        <property type="entry name" value="UNC-13_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999">
        <property type="entry name" value="UNC-13-4A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999:SF4">
        <property type="entry name" value="UNC-13-4A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="8">
        <location>
            <begin position="69"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="9">
        <location>
            <begin position="265"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="387"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002889-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="65069" checksum="54C3487261A3C421" modified="2013-10-16" version="1">MKGKGLGLVRKYIYTNGIQYFAIVYKQLDQVVSDEVVTRMEAGQIPGLTVHPQFAAIEGDKDPADTRPFEIFLALQEFSCLRQHLSIQPQPPEKPLGLENYHEWFTPLVEKWLVISKAKALQRVRSAIQLDSLCEGEKIWPDYNTAPHFEAMLIDAVCSAAMHYSDLIHQALADSGYYEQSGPFRCSDDMCVTVNNLEYIRRSLSEFRPDGQLLHDNAEALLESSLTQLETRAERVLSKLAPLMQTALQKAIFHLAWSPDSLPANQASVPLMEYLDVHLSALNSALLTKNFNRSLTLIWNTVLSELSRQMDTDLDHDKPSTFHSRLHEALQLLVEFFHADGLGLSMESLHCDAFWRVEQRLQYHKTDTDRLIDMFYMQRLHEQYKAQLPGPFGHLSVRAYFNHDSLCVEVLHAKDIIPLDPNGFSDPFVIIELLPKKIFSHCTEQQTNVHKRTLNPVFDECFEFSVSLSQCQADGAMVVFTVMDHDVLTANDFGGEAFLALGTIPGVSDFSTSVDNFHGLKQVDLALMQQHDKNHPILQILETRLNDRVAQDFVRRQRARMASQIAYAKHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL4</accession>
    <name>T1GHL4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002921-RA">
        <property type="protein sequence ID" value="MESCA002921-PA"/>
        <property type="gene ID" value="MESCA002921"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_922252_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="111"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002921-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="302" mass="34340" checksum="F8A988A23EAA7D0F" modified="2013-10-16" version="1">MRQKWVNILRIVYRCDIGYMRKLWPCWKGKVVRSTLSSDLSWSKSPNPAFKITSQKQHTKIACSNLMSYIIESNILIAMIQEPGLTIERLRSLIIMISVSSIKQLRALNLVVSSYFASDDPIPQSIIVSLTENVNFNEDDGIVIGCNASARCNMWGSNVTNERGQYLLDYINTPSLSICNRGNTPTVGFPSEESCVGWADVIDITLSYNSNLFGVRGWMTFSSPIQFVLGILKIRTGLNSVLYLKNILKSIKTKDNTPELLNLRRKLRSTLSSYKSKTLLRTYKKELLKAKRDSWRNFCESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHP6</accession>
    <name>T1GHP6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHP6"/>
    <dbReference type="STRING" id="36166.T1GHP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002954-RA">
        <property type="protein sequence ID" value="MESCA002954-PA"/>
        <property type="gene ID" value="MESCA002954"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294842_0_0_1"/>
    <dbReference type="OMA" id="YIDCERS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF15">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002954-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11480" checksum="C235CB220A24483E" modified="2013-10-16" version="1">MRDRMLGKDDKSYVMYIDCERSWFQHNSVHERRIEGGIQEGSTVGVLLDLDRRSLRFLVNNMPQGSVAFNNLTGVFYPAVSVNRGVSLTLNSGIEPPELDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHS5</accession>
    <name>T1GHS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHS5"/>
    <dbReference type="STRING" id="36166.T1GHS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002985-RA">
        <property type="protein sequence ID" value="MESCA002985-PA"/>
        <property type="gene ID" value="MESCA002985"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226210_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002985-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11844" checksum="982275403CA16F7C" modified="2013-10-16" version="1">MNLRILRYHEETVYTVAFSPDSRVLLSACNMGSLRFMIVDNENASESLTEFDCAVDAAHDLGVCSSDFSKISHTDPEISIKEGPWFWGLPDFGIGPILFIFQILAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHZ0</accession>
    <name>T1GHZ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="22">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 22">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 22">Membrane</location>
            <topology evidence="2 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="22"/>
    <dbReference type="EMBL" id="CAQQ02182685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHZ0"/>
    <dbReference type="STRING" id="36166.T1GHZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003056-RA">
        <property type="protein sequence ID" value="MESCA003056-PA"/>
        <property type="gene ID" value="MESCA003056"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_3_0_1"/>
    <dbReference type="OMA" id="KHTYKKT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000021">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000010">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 21 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 22" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="853"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="894"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="935"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="968"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1008"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="24">
        <location>
            <begin position="38"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="23">
        <location>
            <begin position="130"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="25">
        <location>
            <begin position="786"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="19">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="566"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="647"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="648"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="649"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="734"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="740"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="760"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="763"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003056-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1054" mass="120569" checksum="CB7323E00A796113" modified="2013-10-16" version="1">MSFRNVYKAVRLRLINHDDDVTTSGVTLDDNDRRIIAINSDEPPHHKFCSNHISTAKYSFISFFPAFLFEQFRRYSNCFFLLIALLQQIPEVSPTGRYTTLVPLIFILTVSAIKEIVEDVKRHRADNEINHRQVDVLRNETWVSIRWQELVVGDIVKIQNNKFFPADLLLLSSSEPQAMSFIETANLDGETNLKIRQGVPATARLLEKSDLLNFRGFVECELPNRHLYEFNGVLKEEGRSAVSLGADQILQRGAVLRNTSWIFGIVVYTGHDTKLMRNSTSAPLKRSTVDRLTNTQILMLFIILLVLCVTSAVFNEFWTRSHFKTDWYLGLKTKNFGYNLLTFFILYNNLIPISLQVTLELVRFMQAIFINFDIEMYHEESDTPAMARTSNLNEELGMVKYIFSDKTGTLTRNVMEFKKCSIARNIYTANGPPEHTMLVQNLKNREQSAPIIREFMTLLAVCHTVIPEKNDDGTITYHAASPDERALVDGAARFGFVFDARTPEFVEIDVLGEREKYEILNVLEFTSTRKRMSVIVRAPNGEIKLYCKGADTVIFERLSEQGQTYREVTLDIWKIIPEDVYNEWRETYHKASTALQYRERKVEDASNLIENNLMLLGSTAIEDKLQEGVPETIDALLRADIYIWVLTGDKQETAINIGYSCKLISHAMEIIILNEESLDNTRDSIHQQLRAHDDKEVKYALVVDGKTLKYALSCDLRREFLELCMACRVVVCCRVSPMQKAEVVDLVTQHTKAVTLAIGDGANDVAMIQKAHVGVGISGVEGLQAACASDYSIAQFRYLQRLLLVHGAWNYSRMCKLILYSFYKNVCLYVIELWWAIYSGWSGQILFERWTIGLYNVVFTAMPPFAMGLFDKVYSDDMMLKFPQLYSKQSAKLFNVKIFWVWIGNALLHSVLLFWLPMLAYESDVIWGSGKTGSYLVLGNLVYTYVVVTVCLKAGLATNSWTWLTHCSIWGSILLWFIFIPCYSNIWPTLPFAAVFAGMDTILFSSPIFWMGLFLIPITTLLVDFVFKLVANTLKYADKRRNDPGTAMRESRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI03</accession>
    <name>T1GI03_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D-isomer specific 2-hydroxyacid dehydrogenase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI03"/>
    <dbReference type="STRING" id="36166.T1GI03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003070-RA">
        <property type="protein sequence ID" value="MESCA003070-PA"/>
        <property type="gene ID" value="MESCA003070"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1205979_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF7">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="2">
        <location>
            <begin position="91"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003070-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="24479" checksum="CF7DC24A6921AAE5" modified="2013-10-16" version="1">MCDKYLFFYLKILKTTGLNGGYYTGLQAQAQAAHSTTPHESAPHVGGSGGNIPPPVVPPPVSATPPVSTVPQSQVGLQLGLVSKSVLNEAVGALMWHTIILTKEDLEKFKALRIIVRIGSGTDNIDVKAAGELGIAVCNVPGYGLEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVREAAQNISTLSLQGCARIRGDTLGLVGLGRIGSAVALRAKAFGFNVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIH9</accession>
    <name>T1GIH9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003258-RA">
        <property type="protein sequence ID" value="MESCA003258-PA"/>
        <property type="gene ID" value="MESCA003258"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2040730_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012655460" evidence="1">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003258-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="14384" checksum="25B73699530C1287" modified="2013-10-16" version="1">MKYLILFLILKSIYAQQYQVPQTQSTYNPYQSNSYYTFSDLYPNGYNYYNYYSTPINPVYPGYTYTTASPYYNTYMTGYAGYNAAYPWFNWLYPFNYNGNLNAGQQQVFGRKKRSVLLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ19</accession>
    <name>T1GJ19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CCR4-Not complex component Not1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CNOT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GJ19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003458-RA">
        <property type="protein sequence ID" value="MESCA003458-PA"/>
        <property type="gene ID" value="MESCA003458"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000286_2_1_1"/>
    <dbReference type="OMA" id="HMDHEVR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.800:FF:000001">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000005">
        <property type="entry name" value="Ccr4-not transcription complex subunit 1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.790:FF:000004">
        <property type="entry name" value="Not1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23590">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="11">
        <location>
            <begin position="85"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="10">
        <location>
            <begin position="333"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="9">
        <location>
            <begin position="650"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="12">
        <location>
            <begin position="866"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="8">
        <location>
            <begin position="1228"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="27"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003458-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1570" mass="178313" checksum="9BEBE869052D779B" modified="2013-10-16" version="1">MAGLNLNGPNGAFNFNNVLNTLVSTPSSPSRMMNANANSNSPFPMMMQQHQQQQQQQQQHPQHQQAQHSGVPRLPATPNPAGGHENVLPELNQQVSKEVEDEANSYFQRIYNHPPHPTLSIDEVLDMLQRFKEYQGCREQEVYHCMIRNLFEEYRFFPQYPEKELLITAQLFGGIIERNLVPTFLALGLALRCVLDALRKPEGYKMYYFGIVALDRLSLALAVTYVEYGSIGQEPPNKPQGPVLPPAMAPLLPPASGATPNVQQSPSVESLYRSSSAAAQLQQQQQQKPQPIQQQQSASIASQPISSRAVKSIATATNIDTLLSANTEREEKVTNPPDSVADKTAFIFNNLSQLNLQQKCEEIKEIMTQEYWPWLSQYLVLKRASIELNFHSLYSNFLDAMKNKEVLRLVTRETFRNIRVLLRSEKHVNNFSDRTLLKNLGHWLGMMTLARNRPILAIDIDLKSLLVEAFHKGQQELLFIVPFVAKVLESCSKSKIFKPPNPWTMAIMNVLGELHTEPDLKLNLKFEIEDPDRIRSIELQLSQPKPPAKMQEITQADLQMAQALQQHQSFRNMDDSTMVLLNNPSIVSNPSPSISNDSAGASTGVCGQVVNQPPPEPKFSYIDLVINNYNSLSQYLVINPAAIGILANNPALKHTLTTAIERTITEWLQPLVDRSVRIAVQTTEQIVRKDFALDHDENRMRIAAHHMVRNLASGMAMITCKDQLIQALTTNLRKAFVQNAQSYVGFQDSIEAACNQLAQDNHELVCAFMQKTAGEKALPEIDRRIAHDFEVRKTAREEGRRYCEAESLTYQAERMPEQVRLKMGAASNQQFAVYTELLENYTTQQNFGDVGGNASAGNNTADEMSLVYSDLASKIEAFITNAQQFSTLQLQLSKMHALLKALVNYHRVRDNDSGFILVTRVVEALIEGLTNNISADQIDQMKLYRNICLRTLTMFQSTFGASITERVVTKCVLEIREDVRYNLEAMKLLISSRFINMQHFDNSLIALMESGNYLAITFSMQIVQHFLIDERGNTQITEQDFCNTIEMLARLAQVHRGPEGLGNLIEHLRINHDPNMFSSDRMGPTQFIHSGMNSVRQADCDDPPDLHDKTVFLLKDWCTMNLYGILKTDDLITRFFRQATQFCIDIVYMNPEQNPANHRQMIFKWMDAYVKLIILLFRHSGEASNPATKINLLNKVLGIVVGVLLQDQEVRGVNFQQIGYQRFFTMLFIELSSSDPVIESVMLSVVTAFSHAYHFLNPSCAPGFAYAWLELVSHRVFLSRILAQIPQQKGWPLYCQLLLDLFKYLAPFLRNTELAKPVQILYKGTLRVLLVLLHDFPEFLCDYHFAFCDVIPSNCIQMRNIILAAFPRNMRLPDPFTPNLKAHMLLETGQAPKIFSNYVSNIEANFKKNLDSYLKARSPVTFLSEIRTHLQSSKEPGSVYNIPLINALVFTKNQVPNASNIASSAHIDIFQNLAVDLDHEGRYLFLNAIANQLRYPNSHTQYFSCVVLHLFAQANTEAIQEQITRVLLERLIVNRPHPWGLLVTFIELIKEPVYKFWDHEFVHCAPEIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ66</accession>
    <name>T1GJ66_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02079035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003510-RA">
        <property type="protein sequence ID" value="MESCA003510-PA"/>
        <property type="gene ID" value="MESCA003510"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011160_0_0_1"/>
    <dbReference type="OMA" id="XIDIERC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000056">
        <property type="entry name" value="TBC domain-containing protein kinase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000015">
        <property type="entry name" value="TBC domain-containing protein kinase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF168">
        <property type="entry name" value="TBC DOMAIN-CONTAINING PROTEIN KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="243"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="522"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003510-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="72288" checksum="F99AC93ECEF6DC95" modified="2013-10-16" version="1">MMAELFLDFKFCENWKLSNTTRKILSLQGITNVLEKISGEIDRKKEYDSLPADIRNLLEKCLTSNLKDRLLPEEIIEHDAFRENLEYYLYSNPPKNKENLLNLPLDQIYYLWMLAGGDVHAELKKERLIRNEPPILLIPSVVQLDGKALGAKKSQSYLYDDRIAILSLLNLKERLKKIPPIRYFPLIHTPSCPYNYKDGMEKLPLVIRERDTEYQFHRILLFDRMLKGYPSTREMIVKEAQTDIPPFLRGKIWACILDVIPNGSYSGIDKVTQLKLTDKSKLMFQGVTNTMNGHRKLKRLLKAWVTAHPQYVYWQGLDSLTAPFLYLNFNDEEMAFLSLYKFVPKYLQWFFLKDNSAVIKEYLSKFAQLTAYHEPVLSTHLVGINFIPELFAIPWFLTMFSHVFPLHKILHLWDKLMLGDNSYPLFIGISILKQLKPTLLSSGFNECILLFSDLPDIVMENCVLESQEMYETTPKSLSHREHILREEKPGEWDISDVTLEQIQTELCPRISANDFAELLRYSPEKLCSVDLRGALEFSRLYIEGSINIPFTSVSLSEKNLDALGIPDLNQILEGRIVVVYSNLHENCILVFSKFLVECGVPRVCVLHNGFDILHTIVPNILLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ79</accession>
    <name>T1GJ79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aspartate/ornithine carbamoyltransferase carbamoyl-P binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ79"/>
    <dbReference type="STRING" id="36166.T1GJ79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003523-RA">
        <property type="protein sequence ID" value="MESCA003523-PA"/>
        <property type="gene ID" value="MESCA003523"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830198_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003523-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15147" checksum="45F0A4B8A42AC05A" modified="2013-10-16" version="1">MASVFYEVSTRTSCSFAAAMQRLGGKVIYMDETSSSVKKGETLEDSISVMAGYSDVVVLRHPEPGAVGRAAITCKKPLINAGDGIGEHPTQALLDIFTIREEIGTVNGLTITMVGDLKHGRTVHSLARLLTLYNVTLQYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJH2</accession>
    <name>T1GJH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003619-RA">
        <property type="protein sequence ID" value="MESCA003619-PA"/>
        <property type="gene ID" value="MESCA003619"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075829_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003619-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13707" checksum="334899D4C103D59B" modified="2013-10-16" version="1">MQKSALWFKPFKLCNLRRILVLLSMFVAFQHSTIPCDRPHIFPILLLQAYKARTISVFFGFIFSPQFIAHSLIYLGVNWFLLLTVIGPKPMTTRSSAYVDTVPLSFPNQPYEFFDCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK14</accession>
    <name>T1GK14_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003825-RA">
        <property type="protein sequence ID" value="MESCA003825-PA"/>
        <property type="gene ID" value="MESCA003825"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1857570_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003825-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15766" checksum="EDE11D2FAC88E28A" modified="2013-10-16" version="1">MKNHEGMGDPRKHLQYGKLGNLLGNPEDKIPDMMKAGIYSIGCDSVSRFILSRCAIERRIKKHRCFWINGRAEKSDVAKHLLEENHGMDLSSVKLMKEVQQVYLNESLYICRNKNNMNTEDGPIFSPFSTITGLCPCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKM4</accession>
    <name>T1GKM4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKM4"/>
    <dbReference type="STRING" id="36166.T1GKM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004051-RA">
        <property type="protein sequence ID" value="MESCA004051-PA"/>
        <property type="gene ID" value="MESCA004051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592496_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF227">
        <property type="entry name" value="SYNAPTOTAGMIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9036" checksum="A3F6EF56A2B6844A" modified="2013-10-16" version="1">MNKTLVFAIFDFDRFSKHDQIGEVKVPLCTIDLAQTIEEWRELVSVEGEGGQVLRYQKLSHIGIPSYIIGIRTTAEGITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL00</accession>
    <name>T1GL00_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02102352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004183-RA">
        <property type="protein sequence ID" value="MESCA004183-PA"/>
        <property type="gene ID" value="MESCA004183"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2783231_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004183-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="8074" checksum="ED1DC67882E8C859" modified="2013-10-16" version="1">IQQGFSNAPYTIVSKDDPSHTVRPIQFLDHFACFMHLNLIAIFWDCPVHDYNFSSRLDIIFIPPRVRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL83</accession>
    <name>T1GL83_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spt20-like SEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL83"/>
    <dbReference type="STRING" id="36166.T1GL83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004278-RA">
        <property type="protein sequence ID" value="MESCA004278-PA"/>
        <property type="gene ID" value="MESCA004278"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020200_0_0_1"/>
    <dbReference type="OMA" id="CGRIYHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="3">
        <location>
            <begin position="66"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004278-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="403" mass="46506" checksum="3B2B7D3E7BA229B8" modified="2013-10-16" version="1">MDTENADFDNTSRRSRVPDEEPFDLNSKIKELYLYLLLNDRQKSGRLRHRSFILEHLVATEDIHRIVVNLFPGNKGYSLCYYSGSEIAAAASSSSSSSSGQIPRPNQLVELFHWPYEIDELLYYIDNCQIPTFIADILDERVPNVYYSGCVICEVRDYRQNFLLSSNSCDISFVLLRPTNEEFFAAVHHYQEQNQADEAQTNKFESQLVLSAAEPLCLDPDPEIGRMAINSKYVRESFNTRKMKLRMRKFSQVAINRKRKIDQFTNNPNTGLCDYIQRLRQLHQRTDNKNLLRKNFKILSQPAKPAAVGATAPPNKLNLPDLATPESCDVQAYAKSYELPGKYPNFVPQLVEEYTLETDSHREGCGRIYHIKLSISQRPADLEFLGELYVDRDYRPDQRNAIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM45</accession>
    <name>T1GM45_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM45"/>
    <dbReference type="STRING" id="36166.T1GM45"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004613-RA">
        <property type="protein sequence ID" value="MESCA004613-PA"/>
        <property type="gene ID" value="MESCA004613"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1857571_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="8">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15720" checksum="97877F36C1B6F05C" modified="2013-10-16" version="1">MDDMILTQKQFRSFFTKEGAFAGRSVRGYEYAWPKAIVPILIDDKIGERQRQMIADAINTIASKTCIRIRPATRYDDYAVFITNQHNGCFSEVGFVKKSTQLMNLHPEVAITLQFISMSSCMSLALLMSKVLQIEINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMW1</accession>
    <name>T1GMW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor 25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394636">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMW1"/>
    <dbReference type="STRING" id="36166.T1GMW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004895-RA">
        <property type="protein sequence ID" value="MESCA004895-PA"/>
        <property type="gene ID" value="MESCA004895"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008321_3_1_1"/>
    <dbReference type="OMA" id="YPRIVIA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004895-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="57225" checksum="3FD16FCC75B05FDC" modified="2013-10-16" version="1">MKDDRPAVPKELISMKVLSKAELDPPCSDDTFTWFTFEHSKLYQQHEELFLSAVKRTDTDFLVTLMKKCPYHVNSLIQLSEFCKMTENSSMAVELIERAVLVMESSLHPSFSLTSGLCRLDYRRQENRAFFIVLFKHAQFLEAKACCRTAFEISKLIFNLNLEADPLAMVLVMDYYAIRSRQFSWMVKFYEEWDSIRNLSQLPNMAFSYALALFYLNEMEKSDEALQYALLMFPATLKPLLEELSIKPDQRVESSKYFDSSVSGSCSPALHQLIALYVCRAKVIWRDTNVISWLEKNVNTVLDRIENKDPTTRYVKPPRAILRHIFLCDLKEKVPLASFLAKESEPVMMYDPLPPLDSKNLYDANSSRVAAGSAEGSSNSPISTFLESILPSFQVERNERNRFAELVNNMDREVNRQLDVINDVQQAQAAGVEPNIQYGELRQSLSNIVDAMREFFSSMRVTDSQNADEEGGLSSDDEADNLGDYNEESGIIALDDICGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN55</accession>
    <name>T1GN55_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">G proteins are composed of 3 units, alpha, beta and gamma.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02070282">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN55"/>
    <dbReference type="STRING" id="36166.T1GN55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004997-RA">
        <property type="protein sequence ID" value="MESCA004997-PA"/>
        <property type="gene ID" value="MESCA004997"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_34_1"/>
    <dbReference type="OMA" id="CHQTFSG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="140"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="181"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="225"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="332" mass="36191" checksum="726D09B45EEA5868" modified="2013-10-16" version="1">MRNQLESLRQEAEALKNAIVEARKRACDTSLSNAAANMERIGRIQLRTRRTLRGHLAKIYAMHWSNDSRNLVSASQDGKLIVWDSHTTNKIHAIPLRSSWVMTCAYAPSGSFVACGGLDNICSIYSLKTREGNVRVSRELTGHSGYLSCCRFMDDNNIITSSGDMTCALWNIETGQLITSFQGHTGDVMALALAPQTSQSTFVSGACDASAKLWDIREGVCKQTFPGHESDINAVALFPNGQAFATGSDDATCRLFDIRADQEMAMYSHDNIICGITSVAFSRSGRLLLAGYDDFNCNIWDTMKAERAGILAGHDNRVSCLGVTENGVAVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN88</accession>
    <name>T1GN88_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN88"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005032-RA">
        <property type="protein sequence ID" value="MESCA005032-PA"/>
        <property type="gene ID" value="MESCA005032"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3074723_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588419" evidence="1">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005032-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="53" mass="5675" checksum="9B6426C826733F99" modified="2013-10-16" version="1">MFLFKVICVAFAALCCANAHVITPVGPHLHQVPFAAPHLDVHPLPAFHPAPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNN1</accession>
    <name>T1GNN1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02114455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005189-RA">
        <property type="protein sequence ID" value="MESCA005189-PA"/>
        <property type="gene ID" value="MESCA005189"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161240_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005189-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="11656" checksum="877CE91ABCE71EB1" modified="2013-10-16" version="1">MQVTNNNRLLNPSLSEDSVADIAISKKNSDDDGASSPTNSPQSHRKLSVISDIADQNFSSQNLLNPTTVTGASALPVVLCGILQQDIIGSNGDSKRLYVTPTASTDLLKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPI6</accession>
    <name>T1GPI6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Ubiquitinated; autoubiquitinated.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02185499">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPI6"/>
    <dbReference type="STRING" id="36166.T1GPI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005517-RA">
        <property type="protein sequence ID" value="MESCA005517-PA"/>
        <property type="gene ID" value="MESCA005517"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608783_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072572">
        <property type="term" value="F:poly-ADP-D-ribose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033509">
        <property type="entry name" value="RNF146"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13417">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13417:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005517-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8659" checksum="41DB68F3B5497E0F" modified="2013-10-16" version="1">MAQSISSENEIIVVDDEDSDEAKETSSKEIQETTDPSDSDSIECPICLQNCIHPAKLPCGHIFCFLCVKVSKIKTVSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPU2</accession>
    <name>T1GPU2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02088583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02088584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPU2"/>
    <dbReference type="STRING" id="36166.T1GPU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005636-RA">
        <property type="protein sequence ID" value="MESCA005636-PA"/>
        <property type="gene ID" value="MESCA005636"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645101_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005636-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="7981" checksum="CC3B7ACE805409A2" modified="2013-10-16" version="1">MERLTSHGSIGLSSGSPAYTTHSGGHTGRSGPTGGHSGHGGSHGSASMHHQSLQSDFQPPYFPPPFHHATQSPPQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ12</accession>
    <name>T1GQ12_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005710-RA">
        <property type="protein sequence ID" value="MESCA005710-PA"/>
        <property type="gene ID" value="MESCA005710"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1442611_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="20838" checksum="618902B4A4100F7B" modified="2013-10-16" version="1">MDIIIRDEDISLSQVALYASISFLVVSLLGAFLYITCTKKYRLNWFEKNLLETATEKEEMSQSREALITGNVPYNFDTISECSRGTRGNVSPLSINTDDPTFWVPAKTIMQQQLQVATNPSSSAEESPPTTPGSPTPSSKSLTFSIGSGSYNLGKVEKQFGVPSSRPRVSSMQLNWIILKLILIFIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ59</accession>
    <name>T1GQ59_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02064151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ59"/>
    <dbReference type="STRING" id="36166.T1GQ59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005760-RA">
        <property type="protein sequence ID" value="MESCA005760-PA"/>
        <property type="gene ID" value="MESCA005760"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1416636_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005760-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="20432" checksum="D6EC53BFF41AFBFE" modified="2013-10-16" version="1">MRLTKERDSIQNKWIGYLTMASENDGSSGNGPSQSNQSTPMSSSSVGLASSSSTPQSSERAAGSRRTVYLIDENQKLPETRSSGNTPEPQTPKNNNCNSSNGSSGSNSLTNNNSSSANNTPAAVTESPSTFLMYNRINTMISSGNSPTDPNGSNTSLTGNILNSTANPVDDKLLRKKQEEKSSSIWYEYGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ81</accession>
    <name>T1GQ81_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005784-RA">
        <property type="protein sequence ID" value="MESCA005784-PA"/>
        <property type="gene ID" value="MESCA005784"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834071_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005784-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7861" checksum="A9D29F5863166560" modified="2013-10-16" version="1">MPVLFFGIKIWNIKDINEELLAVFERKIRRIIYVLVCDEALFGNFELDVFNGVDMCTGWKRMSQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ98</accession>
    <name>T1GQ98_MEGSC</name>
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        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ98"/>
    <dbReference type="STRING" id="36166.T1GQ98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005802-RA">
        <property type="protein sequence ID" value="MESCA005802-PA"/>
        <property type="gene ID" value="MESCA005802"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014831_0_0_1"/>
    <dbReference type="OMA" id="CDERRTF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="236"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="6">
        <location>
            <begin position="237"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005802-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="329" mass="36538" checksum="198BBD0BAA9CCA1C" modified="2013-10-16" version="1">MFSPEYEKRILHHFSPVARNLMDSFNSPPKANTIPTSNFSPVISYDRFIPYRANNSWHTNFQSANGSPGSTSTDTKNKQRGELGDSGRDSQAYSCLLKNELFGGNGGASSSMENSLLRNKGDENNENELSSAALKEPQNNNLFQYQSPKKSDYDEHCPYSISPLSAKSQKLLRSPRRAVRKISRIPFKVLDAPELQDDFYLNLVDWSAQNVLAVGLGSCVYLWSACTSQVTRLCDLSSDGNTVTSVSWNERGNLVAVGTHHGYITIWDVAASKQINKLNGHSARVGALAWNSDVISSGSRDRLIIQRDTRAVPHQGERRLNGHRQEVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQB6</accession>
    <name>T1GQB6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQB6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005820-RA">
        <property type="protein sequence ID" value="MESCA005820-PA"/>
        <property type="gene ID" value="MESCA005820"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729569_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005820-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="7916" checksum="AFCD07FF28C856A2" modified="2013-10-16" version="1">MTEMVYGIATQCLVVVKTLSESNVKCVLFRIHQPPASCLMNLSVSLSVWGLCGGFDVPTHHRTDHDRATLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQE6</accession>
    <name>T1GQE6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQE6"/>
    <dbReference type="STRING" id="36166.T1GQE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005850-RA">
        <property type="protein sequence ID" value="MESCA005850-PA"/>
        <property type="gene ID" value="MESCA005850"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545196_0_0_1"/>
    <dbReference type="OMA" id="FIFDEKM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005850-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="8529" checksum="F04838C463ED4281" modified="2013-10-16" version="1">MKATLDEPNLKDKISEDANKTAEKEEFEHHQKELEAVCNPIITKLYQSASGAAVGMPGGFPGSMAGEGVAAPGVGSGSGPTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQG5</accession>
    <name>T1GQG5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">mRNA-decapping enzyme 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02034266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQG5"/>
    <dbReference type="STRING" id="36166.T1GQG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005870-RA">
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        <location>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
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    </evidence>
    <sequence length="497" mass="55624" checksum="4DE693DD9B5F6D3C" modified="2013-10-16" version="1">MKVATTTQQQYRSSSRIQTGTDLLNVIINSAKKSTIKNSPNINNVVVNPSQSEEMSTKQLQQQYLLTQHIPFLQKHVSTLDKILEDWKSYKLTVPTYGAILVSEDLKYCLLVQSYLAKSSWGFPKGKVNEHEDPVHCAIREVYEETGYDITSLIVPTDYIEANINYQTTRLYIVRNVPLDTQFNPRTRNEIKCCEWFPIDSLPTNKSDAISKQINANSFFMIIPFIKRLKKWISEKRSGIDSNNGGSNNNISNRKISSTSSSSTSPVLMTNSTSSSYTTTVDNNITAVAAAAASSSSAPQVAVGTTANATTKSYNNNNNNILNNKNKRQRHKSMGDIDETTSSTTVAAATTTTTWSIFAASASNSSSSKKSNNKQQSSNNSKRQLFNNNNNNFDHLMTNENISSFDLIRKKNAEKKNSINNARQRTKSQQMQQQNQLQQQQNQQQPQNQQQPQKIAVVGSEDGAAKKSEGNVLKFNPNFDSWTNFSFTKNFITNVFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQZ0</accession>
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        <submittedName>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA006056"/>
    </dbReference>
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            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
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</entry>
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    <accession>T1GRF0</accession>
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        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
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    </protein>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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            <begin position="26"/>
            <end position="43"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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    <accession>T1GS08</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        </lineage>
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            <authorList>
                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
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        <property type="gene ID" value="MESCA006455"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10561" checksum="74FD84C4C36AB9DC" modified="2013-10-16" version="1">MIGSDAKLDCFTNCTIHCGVHKCQHPKDFNPDYYSICLGKCNMTFTELMASENCEKFNNTLTFLGILIIFIAIAVYSYYRSRRIAAETGVFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS23</accession>
    <name>T1GS23_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC-2 type transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="T1GS23"/>
    <dbReference type="STRING" id="36166.T1GS23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006471-RA">
        <property type="protein sequence ID" value="MESCA006471-PA"/>
        <property type="gene ID" value="MESCA006471"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080537_1_0_1"/>
    <dbReference type="OMA" id="GEYSHEK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF26">
        <property type="entry name" value="FI22810P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="7">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006471-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20650" checksum="B2FFD54D54C59A29" modified="2013-10-16" version="1">MRFVFPILLSYLPGNFSCFLFSTIIYYWTAQPLELNRFLMFFTISMLIVWVGQGMGLIIGAWFDVVNGTFLAPVSTIPMMMFAGFGVTLRDLPDYLKWGSYVSYLRYGLEGFVGSIYGVGRETLDCEEAFYCHYRYPHKFLSEITMSGDMFWYDVAALSATAILLRIGAFVTLKAKIKAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSC4</accession>
    <name>T1GSC4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006577-RA">
        <property type="protein sequence ID" value="MESCA006577-PA"/>
        <property type="gene ID" value="MESCA006577"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2446995_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006577-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10947" checksum="C5ABD179FFED52CA" modified="2013-10-16" version="1">IKPQAKYPGFILDRKLNWKPNIEERWKKALVGWYTCHEFLGNKFELIPNITRWMYLVAFDLSSHVNWMAYADIFLNSVNWFNYRINGAFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT95</accession>
    <name>T1GT95_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT95"/>
    <dbReference type="STRING" id="36166.T1GT95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006920-RA">
        <property type="protein sequence ID" value="MESCA006920-PA"/>
        <property type="gene ID" value="MESCA006920"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253113_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006920-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12094" checksum="50CE58AB61A68A7E" modified="2013-10-16" version="1">MTSFLLYVFLINFNKYQLVMKFLVFLVSSLVIVRAVKFSPLPEPNPFKLSWKVQGRITNGDEATRFQFKYQVGLRLYADKGTFWCGGTIISERWIVTAAHCTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTC7</accession>
    <name>T1GTC7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02391964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTC7"/>
    <dbReference type="STRING" id="36166.T1GTC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006957-RA">
        <property type="protein sequence ID" value="MESCA006957-PA"/>
        <property type="gene ID" value="MESCA006957"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1241366_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF235">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006957-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="25922" checksum="4FABB23D45A12255" modified="2013-10-16" version="1">MFTRRLQFLLKCTLVLGAFVLIVIIFKNGIQDSEEIAETQFYAENVHVQFIENTHKVNTNDLFNLKSFQHRITPRKECESFQHEKLAVMLVMSYVGHDDLRAAHRLALMEESLNALGILRHSRQGKVHFPSQIDDESSRFGDILQGKFVDAYRNLTYKHMMGLKWAADHCGQFKYVIKVDDDIVFDLYKLRDYLEMGVHGGKFMAGFVLENQAVVRNSQSKWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTG1</accession>
    <name>T1GTG1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02108509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02108510">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02108511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02108512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTG1"/>
    <dbReference type="STRING" id="36166.T1GTG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006992-RA">
        <property type="protein sequence ID" value="MESCA006992-PA"/>
        <property type="gene ID" value="MESCA006992"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036542_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094:SF2">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
        <property type="entry name" value="DUF2358"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006992-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="269" mass="30597" checksum="559E309F8F5EFB02" modified="2013-10-16" version="1">RGLYHYVKQIALLRTVGHLKYAYVKFEVLKITKHPEDLTIKVRWSVRGISGLKVMFQFWKYKLWNLRKAFDDQEAWYDGFSILYLGEDGLISKHIVDRVMPDDNKEPLKSATNDLTAMSTVFRRINFLNSSPHFKFTRLGVSSSISSVVPVLSTKAISTQTLPSSSLNLNWKESFGRNEVLCHQLRSLSTTSTLSQQKAQLEPSPPSQDSSTPSSSPSNNKNNDQIENLNHAFDVLSNTLPKLFIQPLDYSIYSPNLIFENNITGKHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTI4</accession>
    <name>T1GTI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTI4"/>
    <dbReference type="STRING" id="36166.T1GTI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007016-RA">
        <property type="protein sequence ID" value="MESCA007016-PA"/>
        <property type="gene ID" value="MESCA007016"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018837_0_0_1"/>
    <dbReference type="OMA" id="ILHELHY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF165">
        <property type="entry name" value="LEUCINE-RICH TENDON-SPECIFIC PROTEIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="6">
        <location>
            <begin position="459"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="677"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="680"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007016-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="797" mass="89006" checksum="580EA433B1B9920A" modified="2013-10-16" version="1">MNLKNFHNILYYRFNAFFCVVFATTFHLVSSIINEEDVNTNITVGMSSEETSMEEYTTLDTVDFTTTTLAATTTSSSSTETSSSTSTEKSTTTKSVNSLKNPVKYFNKVTPKKRKIDTTVLLVDEPTSEWKCPDIENSRMLECSCAMPHTLRCSGNLHGLSVLAESLRKSPYKVSLLDCTLRNVTFLSDARIFQNVSLHGLVISSGEIKRIHKSALLGISVPLQALGFPSNSLVSVPWSSLVPLTSLERLDLSNNKIKALGPGDFSTLKNLIYMDLSMNQISSISERAFQNLKRLEVLKLSGNRLGDYVSSLKSLSQCFNLRQLDLEGNSLNGPLRTGTLPNIIQNRALQNFTKLTSLSLQHNQIDILQDHAFYGLGSLEFLDLSYNGIVALSSASLQHLSRLRTLDLTHNFLRALTSDLIKPLPSIRDLRLTGNDISIIAKDAMDGAKEIQKLTLQENPLSCDCSIKEFAEWLQNSNIDSSDLLTVTCMTPPRLEGAPLFQVQLENLKCEMDNIEFDNANILEQLDAISKENDTHNILDLSEKIILMDLHYSIDYGLMLTWILNLNQDDYMCNAIFVYKEDNIHEILLDHSPVHCESNIVNNQNTVSVIVPNSSYLSIGESYRFCIVLVQEHNSNSDLVVGCSNITKLQLSKESTWGRKFTRHFSDIASFDKVSRNNDLESDSSEEELDDDDGEELNQNDIQQSSTKKIYLAGRGISVNMNERNETYEQMLVKLNESFLPGLGVGVLVTSVLVLIWGAMKIRSTKPTVAPTSTTCYTANGTRLDSQLGVWKMETDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTJ4</accession>
    <name>T1GTJ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020887">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTJ4"/>
    <dbReference type="STRING" id="36166.T1GTJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007028-RA">
        <property type="protein sequence ID" value="MESCA007028-PA"/>
        <property type="gene ID" value="MESCA007028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1157574_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="26992" checksum="640E2FE0E24A4E0D" modified="2013-10-16" version="1">MKSEMINKLALPDLMETILAEKNEEIDHLKEQLESKEKELQSIFEIENLSQKSHREDDLGKSSARTLSDIVSISEYSEPDVMRRAHLPQESPLVLPDNQNLTTTMDTSKEAVANLTEKRTSDLNVFANLHPASTFDNPHYFQDPSVPIQGINSSSLITSAIAPRKINFSHLTDDSRKAEADETVDFIPSTSSNNHQINFISTSPPCRDNAGHRQSAFTLKSPALISECEQKFDWSRLKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUB6</accession>
    <name>T1GUB6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02044476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUB6"/>
    <dbReference type="STRING" id="36166.T1GUB6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007326-RA">
        <property type="protein sequence ID" value="MESCA007326-PA"/>
        <property type="gene ID" value="MESCA007326"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269683_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053255">
        <property type="entry name" value="EGF-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24047">
        <property type="entry name" value="FI01909P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24047:SF32">
        <property type="entry name" value="FI01909P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588536" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007326-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="23786" checksum="165639E4144DF85D" modified="2013-10-16" version="1">IKYKLFLLIFATYSVFVNCENACPGHESLLVGPGVCVWRCKGELGHCGYNGTCSATGECQCEAGYQQTSNKLLCMPRCLVGCINSDKIRCSAKRYCDCGPTEKFYPYGNKCVPRDNFDLCKGFCKSGGLCDGDENGPFCQCKEGWKNTDASRTECEPDCSQQNNCGVNGYCYLPGMCSCKEGFKYDPTIAENDCVPIDTSTESTSTTEESTSTESSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUE3</accession>
    <name>T1GUE3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Repressor of RNA polymerase III transcription MAF1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02080973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUE3"/>
    <dbReference type="STRING" id="36166.T1GUE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007353-RA">
        <property type="protein sequence ID" value="MESCA007353-PA"/>
        <property type="gene ID" value="MESCA007353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037043_3_1_1"/>
    <dbReference type="OMA" id="DKVCRKT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000994">
        <property type="term" value="F:RNA polymerase III core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.50:FF:000003">
        <property type="entry name" value="Repressor of RNA polymerase III transcription MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.50">
        <property type="entry name" value="Repressor of RNA polymerase III transcription Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015257">
        <property type="entry name" value="Maf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038564">
        <property type="entry name" value="Maf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504:SF0">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09174">
        <property type="entry name" value="Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037240">
        <property type="entry name" value="RNA_polIII_Trep_MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23387" checksum="F4C436EEC8617205" modified="2013-10-16" version="1">MKLLESSRFEALNNALSFQTGDSTVFGRIESYSCKMVAADKALYKRFTAEIPSMNLQALSPPQTLIDLSPNFNRNNSSGDEGIMCDTISRKTLFYLIATLNSAFEPDYDFSDAKSSEFSKEPSLQWVINSINSNLSALAGDQYNVLRTPLWAAVDDEINLNECDIYSYNPDLSSDPFGEPGCLWSFNYFFYNKKLKRIVFFTSRAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUW8</accession>
    <name>T1GUW8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02014182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUW8"/>
    <dbReference type="STRING" id="36166.T1GUW8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007540-RA">
        <property type="protein sequence ID" value="MESCA007540-PA"/>
        <property type="gene ID" value="MESCA007540"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_8_1"/>
    <dbReference type="OMA" id="RVEWISF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="30512" checksum="744F889826790AF1" modified="2013-10-16" version="1">MSSPIVWYVTQAVGIPVGVLSILAVVFYLYLTWKFDYWVKRGVNGPKPKPLFGNFPGIVTSRRNFVYELDDVYKKYKGRERFVGVFTTRQPQIMLIEPDLIQKILISDFRSFLNNEASRWTNREVEPISGVNPFVLTDETWKEKRAEIVQGLTISKIKSSYPILKNVCKKLTAYIKEHEDYSKVGFDTNDLALRYTGEVVCDLVWGFEAGSFKSKENCTFVETSKKTISQTYYSTMFYYASQVLPFIRNSTPYDSFQLNLIGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV09</accession>
    <name>T1GV09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EDRF1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02025463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV09"/>
    <dbReference type="STRING" id="36166.T1GV09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007584-RA">
        <property type="protein sequence ID" value="MESCA007584-PA"/>
        <property type="gene ID" value="MESCA007584"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112189_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056582">
        <property type="entry name" value="EDRF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15000">
        <property type="entry name" value="ERYTHROID DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15000:SF1">
        <property type="entry name" value="ERYTHROID DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23788">
        <property type="entry name" value="EDRF1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EDRF1 N-terminal" evidence="1">
        <location>
            <begin position="62"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007584-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17983" checksum="5468EDB990772C85" modified="2013-10-16" version="1">CPNIEPHENTSNDDVKSKAIQLYSHVQPVQFNKLRCNTNLNLPPSNWLTSSSPISYNLHQALYNSPGFASVISMMVHRVGNTLLIDEFDIQKYLLRKYDEDWKWLRKFIIDNIIKSIGDNERKFYSIKDKSREALQSKNLLSKRKMLKKNARAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV37</accession>
    <name>T1GV37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007619-RA">
        <property type="protein sequence ID" value="MESCA007619-PA"/>
        <property type="gene ID" value="MESCA007619"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1733587_0_0_1"/>
    <dbReference type="OMA" id="SYPTRIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007619-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="17663" checksum="F09CB3D1E7507CD8" modified="2013-10-16" version="1">MDRPETLERSNFNVSYPTRIFSHGWLGGVHAPQTIEIVDSHLSNGRKENYNMIIVDWSCWAMTVNYYFARLRTRKAGKKTAELIDWLHEKTGMPFETTFVYGHSLGAHVAVYHGIQKRRILMIFWKNKEPKRSNLVIHNFTPGVYSSWFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVZ3</accession>
    <name>T1GVZ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVZ3"/>
    <dbReference type="STRING" id="36166.T1GVZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007961-RA">
        <property type="protein sequence ID" value="MESCA007961-PA"/>
        <property type="gene ID" value="MESCA007961"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_649398_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007961-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="423" mass="45932" checksum="BB772D1B2D3ABF7F" modified="2013-10-16" version="1">MIVDEMHEEYPELLKDQYWEEVVPKDLPYTVASEHYRRHEPKGSAENYKIKQADSMYANIIPTKRMRDEDTYADYESVDTPMAERRKSSNWISRQVSRMGSVRSQSTAYSSGGLFNRNRLNSVYSSPESGLPPGITQPPQGKPSLYDKFVNRKSLRNHRQLLKQNSKLTLNGINQSSAPKTSRPRIPTPDVSNEKEGKFNLNFATSTNGNGPSSTASGVAVVAQNLSKNPLVYPTDDKFTSCHLLLSPIKESDCSSSNNTLQPGSPATAALAQACLPKNFQTSLPTTTTIPVTSIAFPTSTGTVSIVPLHSGGIPEITTTPPTHEASLPSIALSPDSIKELLQGDRDTIDGFINFQDNQGNTITRSGSFSLGHKVSTPVKETPPALTAGSATLTTSSSSGALQLTTQPTELILSPIFDQNIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW31</accession>
    <name>T1GW31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW31"/>
    <dbReference type="STRING" id="36166.T1GW31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008002-RA">
        <property type="protein sequence ID" value="MESCA008002-PA"/>
        <property type="gene ID" value="MESCA008002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_2_1_1"/>
    <dbReference type="OMA" id="RCPRIAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF178">
        <property type="entry name" value="LIPASE MEMBER H-A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588578" description="Lipase domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="94"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="357" mass="39898" checksum="076F935F9249176C" modified="2013-10-16" version="1">MTYFRKFLAVLCILAVGRSSEVDLSKENYNKTWMWMSNGNNEPEIAYLVEPPPKARENSIASGIDFEFWGNESSPLTWSYWFPKNKAMRQKRETWSQITERFDPSIETKILVHGWKSSSMSNTIQKRVSEERKAQYNCSNWRNQADNIYYLEPARYTVQVGKAVANLIDMLVEQKSADPKKIHLIGHSLGAHIMGYAGSYSRHRISRITGLDPARPAFEVGITGPEVHLDPTDADFVDVIHSCAGVLGFEKPIGFVDFYPNGGGYPQPGCGEKLVDILRNAGCSHGRSYEYFAESINSKVGFYGYPCSSLDSIRGKNCSIQGDPILMGDQVPKDAKGTYYLKTNSGPGFAMGPLTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWF8</accession>
    <name>T1GWF8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWF8"/>
    <dbReference type="STRING" id="36166.T1GWF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008134-RA">
        <property type="protein sequence ID" value="MESCA008134-PA"/>
        <property type="gene ID" value="MESCA008134"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_767894_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890:SF1">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008134-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="361" mass="41074" checksum="E062C83FEA693157" modified="2013-10-16" version="1">MDGLEKAVNDLVPAFDLKIEQAFVNAKVMEAEGYKSEIDRSFSKMKIYLRNLSSFENVTLKGFSSEMNTEALFHNYHDMFFNNKDSKQKSLFELLTDLFINEGALNRICLNQEFLPQLQIRNYYEQISFVELKANVDFLAFCDDENDPKTERRFSTHLVSSDVQSDYIITGVRIAKKYKTIYLEVQQGKLRPMGHIDQSTISWKPSTDNLNQFISVNHKTKVHISRNYKDTENYVLTHVGFESSNNELFVKMGLTKVNFTAGKLIGQTIYLDSRKSDLPSSMKHNIVENGFLHFVASSKSLDAGQSTVPYLDTGNVEVRGGSLLNGFGLHYKESKGYGGFVGLSVNVYNHNKHIDEIVSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWJ5</accession>
    <name>T1GWJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vanin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008172-RA">
        <property type="protein sequence ID" value="MESCA008172-PA"/>
        <property type="gene ID" value="MESCA008172"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697522_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040154">
        <property type="entry name" value="Biotinidase/VNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043957">
        <property type="entry name" value="Vanin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609:SF14">
        <property type="entry name" value="BIOTINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609">
        <property type="entry name" value="BIOTINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19018">
        <property type="entry name" value="Vanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vanin C-terminal" evidence="3">
        <location>
            <begin position="333"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="396" mass="44095" checksum="6D3B50B3127D4CEF" modified="2013-10-16" version="1">PNAWFQELPFLTSLELFQGWSQANNVSLLAAGLSSPENGNSGSGIFSGNYGSLVSGIHGTPERLIYTAKVPKKDSKYVPKETPKPKAHKNYPDEKRLQPPPRQVQSTFQKTLPSDPYYKAGVVEFAPILAHSTNSSIVEQLEGVRTIIQDPNAGNLDILVFPEGALSSEGLTYVPDPHEQVIPCEELDYDYSLSEISCYARSIQAYIAVNVHEKVQCLGDYQYFISPNAWFQELPFLTSLELFQGWSQANNVSLLAAGLSSPENGNSGSGIFSGKYGSLVSGIHGTPERLIYTAKVPKKHSKYVPKETPKPKAHKSKPNTPFVIKKNFKTILTKRDYNLDLFTTFLLDPVEDNFNKTLCHGDLCCDFLIVQTTKKLKTDVKFVNNLYIGYQYRXTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWK9</accession>
    <name>T1GWK9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008186-RA">
        <property type="protein sequence ID" value="MESCA008186-PA"/>
        <property type="gene ID" value="MESCA008186"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375163_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008186-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="11218" checksum="BD8659E4AEB694E7" modified="2013-10-16" version="1">MMFLMPKQIAKSKFYKAAMPVLVKNFEPSFNKHVFVQLTFYLGLWKKTQESMDLMYQVLQDNLLSYTTPSRKSSIVWNLFVGFLYTHGVAQHYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWP8</accession>
    <name>T1GWP8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144066">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWP8"/>
    <dbReference type="STRING" id="36166.T1GWP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008226-RA">
        <property type="protein sequence ID" value="MESCA008226-PA"/>
        <property type="gene ID" value="MESCA008226"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_605933_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008226-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="452" mass="50626" checksum="DCD8819024C43C49" modified="2013-10-16" version="1">MIEKIPGVGGAKQYNKFNINSSSKKRGGIVYEKQNLNNSKNISRKNNQQLPPPTNIISSSRKTVATSAAATTNSVALNTTNLKKIKNNKNLNKNSNFNRKRNFAEVRQIKRGYRHLLGRCSDIQVGNNTTNTKNHQQKKNQDKKQRRKSSFASSSTLISSWDSIEERINLLEKFINYEYYVKSEEDATDKTEENMRRGHQHHPRFAGQRKSTVVIEDVLAALNKGDSGGASSVGDEDGISAKYITSESSSNSSSFYMDKSLDEHSFPAATSKSLDFNLNKSFDDKVSTTSPSATSSNNNIDTVVEDAEVEEKELPSKPAKSKSKSTKKKSSSTKSDTKKREKKSKSLPDGSQSLPAPEDDVETEELAKLRCTSERTEVVAERETRRNKNRCSDYPGLAFGGSIFSSDTMMKFNIIRNELHNIMKNDLVLEKERYKDIGDDLDTAFVELILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX12</accession>
    <name>T1GX12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX12"/>
    <dbReference type="STRING" id="36166.T1GX12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008350-RA">
        <property type="protein sequence ID" value="MESCA008350-PA"/>
        <property type="gene ID" value="MESCA008350"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_4_1_1"/>
    <dbReference type="OMA" id="SYHIMHT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="4">
        <location>
            <begin position="129"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008350-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="524" mass="60785" checksum="D2120687CBFED618" modified="2013-10-16" version="1">MNTNKNVQFHPLISSIQKVVLYETKSRLYLVGSNNRETRFKLLTISRVSSDDSELKVYEYPNEYSNKDIRQIIGNIGNAKVTITKRTKVAFIGNHPIYTIKDTVMIKVNQPLPSGKNPHPFEQRYVKMFQNIDLKSNFYFSYSYDLTRTLQHNLSAPRYVNPDPSNIDIEKEEPFPDFTGSGDFAYRINSRKRFVWNDFLLKPMKNIVLKDWLLEITHGYLSQSCMNIFGRPVYILLMARRSSRFAGTRFLKRGANFHGDVANEVETEQIVQDGSRLCSFTQMRGSIPSHWSQDISKMVPKPPIGVDISDPFSQTPAKHFERLLFHFGSPVIVFNLVKMRERRKHESILTKEIKTSIKYLNQFLPPQHKIKYVHFDMARKIGFFSIDKNEALQFQNGIIRTNCVDCLDRTNTAQFSIAKYALGFQLYHLGFLHSPKLVFDSECVNLLESLFEDHGDTLALQYGGSQLVHRIKTYRKTAAWASQGNDIMQTLSRYYSNTFSDTEKQHAINLFLGYYIPHKNIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXC8</accession>
    <name>T1GXC8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02056613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXC8"/>
    <dbReference type="STRING" id="36166.T1GXC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008474-RA">
        <property type="protein sequence ID" value="MESCA008474-PA"/>
        <property type="gene ID" value="MESCA008474"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062806_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF187">
        <property type="entry name" value="CYTOCHROME P450 4AE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008474-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27696" checksum="6D2B98C97B810F26" modified="2013-10-16" version="1">MDLKVLVLFTAIAAILLKFILNYLKARNSVKNIDGPTSIPLIGNIELAFLTNTNQLQWFFKNAKNFQKYFKMWLGTELWVVIYDPEMAEKVLTNPKLIKSNDYVFLKPWLGKGLLLNEETSMGLKMNSQNESNVPYVKAVNDLKVVSETTQKVISERRKYYAERPDELQESTNELGVRKRMPLIDTLLKAHVDGQPLSNRDIEDEVNTFLFAGHDTTSHTLGYFFYNVAKHPEVQEKIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXM8</accession>
    <name>T1GXM8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methionine aminopeptidase</fullName>
            <ecNumber evidence="6">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02385003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXM8"/>
    <dbReference type="STRING" id="36166.T1GXM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008581-RA">
        <property type="protein sequence ID" value="MESCA008581-PA"/>
        <property type="gene ID" value="MESCA008581"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_1_5_1"/>
    <dbReference type="OMA" id="SSKMYVM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF8">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="7">
        <location>
            <begin position="147"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="378" mass="42241" checksum="3122B94760B68405" modified="2013-10-16" version="1">MLMMMIKTLRDFCPVTPPCILENLLPTSKIINLIIEEKPQKYQLYPLVPKLSQNQIVKKNQTISAVTMQSFRNLGVNIASKHCHFQKASFWNTKKKPDIHNFVELGTVSGELEVPEHIEKPPYYYEFSKPGSTIGKPEIKNPEQIQRMRESCKLAANILKSSQQLAVEGITTDEIDRFVHNEALKANAYPSPLRYSGFPKSVCTSVNNVACHGIPDDRRLMDGDIINIDITVFFNGFHGDCSKTYLVGNVDERGRHLVKSNEECLNKAIGLCAPSVPVNIIGGFCESFAKLSKLKVIPAFVGHGIGSFFHGPPEVLHFIMQTGMTFTIEPIFTTGEEQEIEIQEDGWTAMTVDGARTSQFEHTVLITESGVEILTLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYF1</accession>
    <name>T1GYF1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYF1"/>
    <dbReference type="STRING" id="36166.T1GYF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008876-RA">
        <property type="protein sequence ID" value="MESCA008876-PA"/>
        <property type="gene ID" value="MESCA008876"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834047_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008876-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7586" checksum="A0B4ADE411BA3A04" modified="2013-10-16" version="1">MDSDKVLVMDSGQMVEFGTPFDLLTRSEKKVFYGMAQQTGKYMFDILFKVAEDAQGNCYKFEDVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYZ1</accession>
    <name>T1GYZ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYZ1"/>
    <dbReference type="STRING" id="36166.T1GYZ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009079-RA">
        <property type="protein sequence ID" value="MESCA009079-PA"/>
        <property type="gene ID" value="MESCA009079"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1598665_0_0_1"/>
    <dbReference type="OMA" id="NERSCEI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF8">
        <property type="entry name" value="ACROSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="55"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009079-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="18417" checksum="A8E82AF4BD5A5BC6" modified="2013-10-16" version="1">LSNEICRNSGYVAGTSVCCPIEETTSNPRTNPDNGAVSLNGPECGLSSVFKFRRVVGGEPSELGTWPWITLLGYDVLEGSPFKCGGSLITKRHVLTAAHCIRKTTTVDINIVKMDRHPQYNSRNGKNDIAILHLESPVSYTERIHPACLPKDPPLRTRSFVDYMPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZF4</accession>
    <name>T1GZF4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZF4"/>
    <dbReference type="STRING" id="36166.T1GZF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009254-RA">
        <property type="protein sequence ID" value="MESCA009254-PA"/>
        <property type="gene ID" value="MESCA009254"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2190538_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009254-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12627" checksum="5373E2D780E4C235" modified="2013-10-16" version="1">MLVHCNDGGEEEESYHVYGYLKKLRQARKGLIENLDQYKFVYDTLEENIICGKTWFPVSELSDRLKSKAKKNAASKMNEYQSEYLLICRQTPRFSIGDCAGGHRADNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZM4</accession>
    <name>T1GZM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02117229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02117230">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009328-RA">
        <property type="protein sequence ID" value="MESCA009328-PA"/>
        <property type="gene ID" value="MESCA009328"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1637354_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009328-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18318" checksum="D84B6377555A1ACA" modified="2013-10-16" version="1">MIEAFANHLGSHLGRHIIYWALGQDGSEGRHRQLELLDKIQGPFERGVHVLDNLFTLDEEDFNNWGSGYAHHCDIVEEEGDESNVENLDGNERNQRAMQTANGTNSTNGRIVYCQRCCGALDESCLLYVGDHIDDTVRGYIMFCIFHLCEPPHKLCGVSPFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZN3</accession>
    <name>T1GZN3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7083 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009337-RA">
        <property type="protein sequence ID" value="MESCA009337-PA"/>
        <property type="gene ID" value="MESCA009337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1497925_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055510">
        <property type="entry name" value="DUF7083"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23309">
        <property type="entry name" value="DUF7083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7083" evidence="1">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="180" mass="20545" checksum="B93B05619D7B83B4" modified="2013-10-16" version="1">MQLSVSASSDSILEFSYDPENNATIDIRYKRYESVFPKVRLLTSKLTNQDFEQFANSFSPKKPHDFSLNEGVEKLKSSQQIFLAVAGPTLKPLKKPHNIEAVPRRPCWLNALCERLPTHRNKFSNLEFFDKQNNVPIVTILFQIARSTMLSATHIKILTTPNILDGRFLRGANIDSDHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZV3</accession>
    <name>T1GZV3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381545">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZV3"/>
    <dbReference type="STRING" id="36166.T1GZV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009410-RA">
        <property type="protein sequence ID" value="MESCA009410-PA"/>
        <property type="gene ID" value="MESCA009410"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253373_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009410-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="221" mass="25372" checksum="FE7B4213E51A06D8" modified="2013-10-16" version="1">QYISDETIHNVQWSAALFTSNIFYHAPQQQVSNPFRRQINDGFKPKQQQQQQQFFVQQSQSIEVPFNQKNNLINQRPPAQQNRFPVPNQFAPPQPKSNPVFQRSLENQLRIPQQPYQVLPSTQVSSVVPKEEFHSQNFLTLRNHKHVVQGTLFSAGMRFYQVKDSLANGNSVILQKTSAGKIKVNDAHMVSSTSQPQTVLFTPSTDSYNRQKKSADINCYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0C9</accession>
    <name>T1H0C9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0C9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009604-RA">
        <property type="protein sequence ID" value="MESCA009604-PA"/>
        <property type="gene ID" value="MESCA009604"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662793_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009604-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18006" checksum="1ABDC62CFCBC68A0" modified="2013-10-16" version="1">MSSNKKKKWFLEEEEQQQEEEGEKEKRRLSYKKTWFGVMCLLKHTWKKPSHITGGATKLKGSNSDIISPRQPLSQANTAISPAPASTVIPHQSPNAPGGVANTNTLILQQPLHQQTIVQSSQSLQQPHHQQLHHQQHPTQHITAQIHTMKDQPQQPMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0H5</accession>
    <name>T1H0H5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-Nucleotidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H0H5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009653-RA">
        <property type="protein sequence ID" value="MESCA009653-PA"/>
        <property type="gene ID" value="MESCA009653"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005854_7_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.780.10:FF:000004">
        <property type="entry name" value="UDP-sugar hydrolase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF32">
        <property type="entry name" value="APYRASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="9">
        <location>
            <begin position="229"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009653-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="411" mass="45508" checksum="08B825ACC4A26D85" modified="2013-10-16" version="1">MLVANMDATDEPDLQNKFLKSTVLVRSGRKIGVIGVILETTYNLANTGKLKFLNESITIKEEAAKLRSEHPDLNIIIVISHCGYDVDKXXXXXXXXXXXXXXXXXXXXXDVDKIIAENAAEDVDVIIGSHSHTFLYPSDLPEPGPDKKRGDYPTVIKNSKDGHKVLIVQAAAYAKYVGDITVYFDRNGEVVTWEGAPIFMGKDVPEDEETLKAMAPWKSIVDEQGKRVIGRNRVRLDKGSCTYQECNLGNFFADSMVHAYATEAVESKQNWTKASIGLVNAGALRVPLEKGVLTYSHLVTMSPFENTLTAFSLKGKYLKEALEHGLQYFNIPAGETSSYIMSQVSGLRMTVKVTNPVGSRVTELKAKCHQCSIPIYEDVKDETVYRIVAPNFLANGGDGYEALEKHKFDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0U7</accession>
    <name>T1H0U7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0U7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009789-RA">
        <property type="protein sequence ID" value="MESCA009789-PA"/>
        <property type="gene ID" value="MESCA009789"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457362_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="9434" checksum="FC50A29C31EE3BB5" modified="2013-10-16" version="1">MVGSSGERHQTKSTTKLETISQKPRCMGKSSSSSGRAVACASCKSFSIWICHSRSILTSGGNKAGMATKSKFGSPTNFLANQRKGFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Y5</accession>
    <name>T1H1Y5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Y5"/>
    <dbReference type="STRING" id="36166.T1H1Y5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010209-RA">
        <property type="protein sequence ID" value="MESCA010209-PA"/>
        <property type="gene ID" value="MESCA010209"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239663_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010209-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11645" checksum="E4F91E4F4F8E4F3C" modified="2013-10-16" version="1">MTVEESSFFLKVAFVSSVSKEKCSSDNRNSGKVTVEELSFFRYYPIADYDSPHITTVQKCRRQCLSGSRTLTDQLLQGPVGIGIGVVFGFLYGLALVVLPSRRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X8</accession>
    <name>T1H2X8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2X8"/>
    <dbReference type="STRING" id="36166.T1H2X8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010584-RA">
        <property type="protein sequence ID" value="MESCA010584-PA"/>
        <property type="gene ID" value="MESCA010584"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006527_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="1">
        <location>
            <begin position="2"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010584-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13930" checksum="13C7F343FD4C45B1" modified="2013-10-16" version="1">MISRPQDDFKHTGHVGIDGASFGDISFLSNPNSSSPQNFNNHVPRQIVTPYKPSEDIEQTPLLLPPTPTSPDSLQTASGYFSEIIFITPQQIQHLSIRLMIHKTQTRIKLLISHPPITIIIHFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Z1</accession>
    <name>T1H2Z1_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Z1"/>
    <dbReference type="STRING" id="36166.T1H2Z1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010598-RA">
        <property type="protein sequence ID" value="MESCA010598-PA"/>
        <property type="gene ID" value="MESCA010598"/>
    </dbReference>
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    <dbReference type="OMA" id="ENHEWKS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF112">
        <property type="entry name" value="LD11247P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010598-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="295" mass="32630" checksum="BA67057ABB0242F7" modified="2013-10-16" version="1">MFSFLSIGWGLISDIDIESERLRYMGHPRFSLWSVRRLIGLRTYHGKISYIPASSELLNASSTTDNPPNRTGIKHSISCNTYLTEEGATSDSVDGEVIALETSTDGFFDTISHRQRFDSFYSATSRKTAYYSVAGSDYKSINGSIKGGEEGEDFPDHDKVYNLDNPSNPKDVQVFGPSDYVMVHATYQTHLSSDVFFVPSSKLSDGIIWLVIIKAGVTRPALLNFMMGLSSGTHLPDKEHNFFKVIPVTAFRIEPTGSDGILTVDGERVDYGPIQGEVCPSLAKVMVPEKFVKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3A4</accession>
    <name>T1H3A4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3A4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010724-RA">
        <property type="protein sequence ID" value="MESCA010724-PA"/>
        <property type="gene ID" value="MESCA010724"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690636_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010724-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8609" checksum="CAC9C5A5AAB17294" modified="2013-10-16" version="1">MHAPSTNCILQYRNKSCSCPRRLLEKANIPPIDYINYITYINYKITTAIDERGESVVLQVNGEKTEYKLSSFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Y5</accession>
    <name>T1H3Y5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium-dependent multivitamin transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Y5"/>
    <dbReference type="STRING" id="36166.T1H3Y5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010982-RA">
        <property type="protein sequence ID" value="MESCA010982-PA"/>
        <property type="gene ID" value="MESCA010982"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_7_0_1"/>
    <dbReference type="OMA" id="SAMAEIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010982-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25140" checksum="4BCAA3B197C1D198" modified="2013-10-16" version="1">MASFSVWDYLVLGIVLMISAIIGVYYKYSGGKQKTNDEYLLGNRSMSTIPVSLSLMASTLSAIAILGGSMESYQNGTGYALFYVGVIISAPIVNFLILPVFYNLQVSSLFEYFTKRFGYSTRIYMSVIFTGQTILVMGIVLFAPVLALEAATGIDRNTAICLIGLAGVNFNVFEKARYCVLTPMHASLDCEFLQHQYNMYTYNTDEVKDLFYNGLSLWKTILCCLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H449</accession>
    <name>T1H449_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02186990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H449"/>
    <dbReference type="STRING" id="36166.T1H449"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011050-RA">
        <property type="protein sequence ID" value="MESCA011050-PA"/>
        <property type="gene ID" value="MESCA011050"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_0_3_1"/>
    <dbReference type="OMA" id="LGIEMCQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000179">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="7">
        <location>
            <begin position="42"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011050-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="351" mass="39362" checksum="9D83B2243DEF06A6" modified="2013-10-16" version="1">MKFFQADRIKNDGYPVETHTVITSDKYILTLHRIPPKNSNSPVIFLMHGLSSSSSDFVILGPKRALAYKLSDAGYDVWMGNARGNTFSRKHEILSPYTLAFWRFSWNEIGIYDLPAMIDYILEKTQNQQIHYIGHSQGTTTLLVLLSEKPEYNKKLQSAHLLAPAAFMGHMKSISIISTCPSLSVPNPAAATGSIPIMTTDFEKDIASFSCDVIPGLKEFCKLSIFEISGFDHKNLNESLLADIYATSPAGASASQLYITVKNSFLENLENLIMVPPSYSLNNTLVPTFLYYGQNDFISAPEDVLRLRNELSNVKLFYKVSDPMWTHLDFIWATNADSMVYEKVLKGIESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H491</accession>
    <name>T1H491_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF243 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H491"/>
    <dbReference type="STRING" id="36166.T1H491"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011095-RA">
        <property type="protein sequence ID" value="MESCA011095-PA"/>
        <property type="gene ID" value="MESCA011095"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051251_1_0_1"/>
    <dbReference type="OMA" id="QPQAVYQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="76"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011095-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="253" mass="27447" checksum="FAD0AF19B1042125" modified="2013-10-16" version="1">MRGFIFEFVCHFIGFTTRIQLPTHQSTSFGGGSFVSTGSSGNFVANTASSGYIAPTNAGISSYSSGHQQQSQVKEAIVSKRFFIHSAPEEHDDDYKEKTISVGTPRKNYNVVFIKAPHKTNKGGKIKIVPATNEEKTVIYVLNKKTDASDIQAEVQEVVQTTHKPEVFFIKYKTPEEAHHAQQQIQAQYDALGGSTQVTDEGIAPVSSVIGSLGQNSHAEASSGPNYEHSASSHHIQSGGHQSGNGYLPPNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4N7</accession>
    <name>T1H4N7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein O-GlcNAc transferase</fullName>
            <ecNumber evidence="3">2.4.1.255</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02155523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4N7"/>
    <dbReference type="STRING" id="36166.T1H4N7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011252-RA">
        <property type="protein sequence ID" value="MESCA011252-PA"/>
        <property type="gene ID" value="MESCA011252"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893041_0_0_1"/>
    <dbReference type="OMA" id="XMKERIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000013">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037919">
        <property type="entry name" value="OGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029489">
        <property type="entry name" value="OGT/SEC/SPY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13844">
        <property type="entry name" value="Glyco_transf_41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="19"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="9">
        <location>
            <begin position="168"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011252-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="312" mass="35117" checksum="E1D13D9F9501964E" modified="2013-10-16" version="1">VKEAEECYNTALRLCPNHADSLNNLANIKREQGCIEEATRLYLKALEVFPEFAAAHSNLASVLQQQGKLNEALLHYKEAIRIQPTFADAYSNMGNTLKELQDVTGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIQSYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYEARMKKLVSIVAEQLEKNRLPSVHPHHSMLYPLSHDFRKAIAARHANLCIEKIHVLHKAPYKFLRELSTPRLRIGYVSSDFGNHPTSHLMQSIPGLHDKSRVEIFCYALSADDGTTFRGKIAREAEHFIDLSQIPCNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H653</accession>
    <name>T1H653_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H653"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012186-RA">
        <property type="protein sequence ID" value="MESCA012186-PA"/>
        <property type="gene ID" value="MESCA012186"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_825330_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF74">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="16"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012186-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="337" mass="38808" checksum="ABDF94C53BE0BCCC" modified="2013-10-16" version="1">EYLDKFSLKKLPIENIRNYFGSSIGLYFAFLESYTNALIVPTVFGILQYSSNLVLTVCAFYMIWTTIFLELWKRRCSTFSYRWGTIDMTSLDKPRTSYYGELKPDPITGKLTPHYPMKYTYMQMYLVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFPTMPALTFVLFEHILLIIKFILHMAIHEKPRSIRLAQLIDDHETHQALKHLNLLKVEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6L7</accession>
    <name>T1H6L7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Negative elongation factor E</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6L7"/>
    <dbReference type="STRING" id="36166.T1H6L7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012350-RA">
        <property type="protein sequence ID" value="MESCA012350-PA"/>
        <property type="gene ID" value="MESCA012350"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033102">
        <property type="entry name" value="NELFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17250">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17250:SF0">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="92"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012350-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18592" checksum="244BBCE6010378A6" modified="2013-10-16" version="1">QEPEKPISLKRPADARDAREVARKLIKSGAIPAIQKQQTKQDQTSFKRPKGQERKRIPSEAGVSSYQPFSSTQNDVAQEFLINEMIKEEPVRGPNLYQKYSTEREKDEKVDKKTSSSCRQYNKPRAGNTIFVSGNKVNEEFLKKHFSNFGSIVNVSMEIEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Y0</accession>
    <name>T1H6Y0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6Y0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012462-RA">
        <property type="protein sequence ID" value="MESCA012462-PA"/>
        <property type="gene ID" value="MESCA012462"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109343_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="251" mass="29306" checksum="A08D43316185EADB" modified="2013-10-16" version="1">IKRGDVILSEKPFVYCLQSKYKNERCDFCFQLNDVKRCTACNYVHYCNKICQKNAWKQHKIECNFLKKIHPRVVPDAARILSRIILKLNNGGDMEKGYYTETSYRKFRDLMSHYAELKNDPKRIDHLESLXXXXXXXXXXXXXXXXXXXXXXXXXXIFISYIDLLNTTENRRKELQENYYFLCNCSKCLDKQFSESMEGAICPNKNCKAAVNVKYNNCKLCNAGVTPKLRNAYVEAMEFTKSQLTEMRDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7B7</accession>
    <name>T1H7B7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EnsemblMetazoa" id="MESCA012655-RA">
        <property type="protein sequence ID" value="MESCA012655-PA"/>
        <property type="gene ID" value="MESCA012655"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256788_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012655-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11875" checksum="5453F50EB831534B" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKFKMEYFFILEHSEIQSMYSGSIIGTEMVSRYFRFTLSITDPGYSDIIGLLPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7D9</accession>
    <name>T1H7D9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7D9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012676-RA">
        <property type="protein sequence ID" value="MESCA012676-PA"/>
        <property type="gene ID" value="MESCA012676"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_569355_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012676-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="480" mass="54267" checksum="3268389636569BBA" modified="2013-10-16" version="1">NCKSFLLFLKFPSMARKIIGPQLDNRVIVVARSSQWQVREFLSSPDSSNLINLLVIGESLTSDPSRERPYVLYTHKLYTDGLGSNSPVVLTSWIKGMLTRPHINLFPTKLAEGFAGHRFTVSAVNQPPYIFRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGWESWFANSTHEATDKLYRKMEFVNDINEGIGNVTKSFFWNYAFLGSRGQLEYLIQSNFSDDTLSKRSALHLSEECFAPFQIAYTFPKYSVYENESMIXFVNYSGILNKITNEVSWNLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA20</accession>
    <name>T1GA20_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Dynein heavy chain 2, axonemal</fullName>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="28">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GA20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000063-RA">
        <property type="protein sequence ID" value="MESCA000063-PA"/>
        <property type="gene ID" value="MESCA000063"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000038_4_0_1"/>
    <dbReference type="OMA" id="ILKNDMQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000008">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000002">
        <property type="entry name" value="Dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000008">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056759">
        <property type="entry name" value="DYH2-5-8_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25007">
        <property type="entry name" value="DYH2-5-8_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain tail" evidence="17">
        <location>
            <begin position="169"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein axonemal heavy chain 2/5/8 coiled-coil" evidence="26">
        <location>
            <begin position="976"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="18">
        <location>
            <begin position="1159"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="19">
        <location>
            <begin position="1693"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="19">
        <location>
            <begin position="1801"/>
            <end position="1962"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="23">
        <location>
            <begin position="2134"/>
            <end position="2176"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="21">
        <location>
            <begin position="2519"/>
            <end position="2729"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="20">
        <location>
            <begin position="2779"/>
            <end position="3034"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="22">
        <location>
            <begin position="3108"/>
            <end position="3325"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="16">
        <location>
            <begin position="3550"/>
            <end position="3608"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="24">
        <location>
            <begin position="3645"/>
            <end position="3784"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="25">
        <location>
            <begin position="3792"/>
            <end position="3900"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="25">
        <location>
            <begin position="3904"/>
            <end position="4050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2993"/>
            <end position="3034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF25007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000063-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="29">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4054" mass="467042" checksum="480DF7505A614FAE" modified="2013-10-16" version="1">MATGGGDHLSLLSSEDSADDRGRKRAASRNAKSSSANILRRRVGQTGDVCSAHDDPLLPEPERLARGNVGLACLGFPLVSVFDLSYFLREPNQIFTVEGFHDENVYAPIFHNYTEWNDNVKLRFCNAMDKFLCYLTGLHYKMSGMTYLYVPHVIKKLESGTAHTDREFVKNVEEVVKYWNGQIRTCLNDNQLTVPHDLVVPVEEYEFWLYRFEVVQGLHRQLMHEDVQSILNLLRKTQSVHIKGIEFLISECQSKMLEAKSNIKYLKLFVDPCKELDKCQHPLKIPPLIPKIVHIFRYIWLNSDHYNTVELIGNLFKNLSNQVIKYCLEEVNITKIFSGFPRFGSKCVTCPLTNPQIGWPLDKARIFNHVDAFVERLNDVTDICKAMIVFARMDEVEHITMPQFCGTRGNEFQKTVRQVEQNFSEAFDKLCQVSKDIILNVHKNQWYEDVVEYRHVIQNLEEIVQRLISDVFEYTCNTEEELESLAFLNYFTYRENLRKAYLHQVSEMWKKFSLELKRTTSQVMDRQKSRPSWLPKYAQSALWYRSQIEHYQWIYNRLILAEWLPNVPSRQTAIDEFLIFKQDLEAKTAKSFEDFVRSVPDTILNRLERTLLVRSKVQQGLLECNIDRSILPICSNAQIFAEMGFELPKNVSKIYEKILGALAPGERKLFRALLISCERKISPGVFKLTWGGEMSDAYIADCAKHTGKIQETLDTYKSGNLEIAKLCEKICDTPILKINQYGTVELKPFSDNLYKFMKRGLKSHVENFKQIKELLAAIFVGFEPAIDDMRAEWKEYVRRFDEMIELALLTSVRNSLRNIYLPIHGDGTMGPTPIILMFVNIEKKQLEFSPSFDELTNAFQKLYDDIIEETIPFPRLVVKESISSKIVDDTDSQELKDQIDSEVKFEINALIAYLDVWLVYKDLWVYEIKEHLALFEEKKPESVDYERMIDMYGDLADKVAIQETITSVHFVIVNSQNLRNALLDIIETIYRYMEANIKAVSVVPRTIAEMKNSTKLYQRLVDEVEGKQSTFPGIYDLFRVLDKYKIPVPEDTRSRIVGLEQAWAGYLRKLLDVDEMLGNAKEEYKQNLLLQAEKFKHIIKSFLDGFLVNMPTTSSIPPRKALRYLESIRDKLVEMLNIEKKVVADLVVFNVNQPDNMDLKTIDSELKILEEIWEFVNEWDISWKQWRTGNFWQINVDEIEDIVMHLFKNFTRLFRTYRDKNWEVLETTRNVVDGFRKTLPLITALKNPAMRPRHWERVRDVMKVDFDENADSFNLAQIIELDFQAFSEEIQDISNAATQELAIENGIKNIAEIWKTQGFEMVFYRDGIYRIKNVDDCFQLLEEHQVQISTMKSTRFVEPFIKEVDFWEKTLSYIMETLETALVVQRQWLYLEIIFGGEDIRKQLPEESFRFEAISQEWGMLSTRMYKGKTAQKATHFKPQPFLLNKLQRIDEKLEIIQRALEIYLETKRQIFPRFYFISNDDLLEILGNARRPDLSRAGKSTTQGQNCVASHGNVRDDGEYVEFINAMFIDGPAEKWLAVVEDRMIQCLKEILKLTKTSLKKMMNNREKWLSMWPGQLCQTTSQIQWTADCTRSLIHCKMIDEKKPLRKLKKKQNKVLMRLSESSRKDLPKILRLKVNTLITLEIHGRDVIERMYKNNCKDVSHFEWFSQLRFYWDRMRDVCVIRQTNCEHTYMYEYTGNSGRLVITPLTDRCYITLTTALHLHRGGSPKGPAGTGKTETVKDLGKALGIWVIVTNCSEGLDYKSIGKNFSGLAQQGAWGCFDEFNRINIEVLSVTNFYDVPDNAIIAENLLFSDGFQNTRNLARKVFTLYELAKQQLSKQYHYDFGLRSMVALLRYAGRKRRQLPNTNEEEIVYLAMRDMNVARLTANDLPLFMGIMSDIFPGVSLPVIDYSEFKVCIDDYLKETGMQLVPIAPKKVIELYETKNSRHSSMIIGNTGTAKSVTWKTLQGAFCKMKALGMPGWENVVVYPINPKALNLAELYGEYNLGTGEWMDGVLSQIMRTVCADEDLTNKWILFDGPVDAVWIENMNSVMDDNKLLTLVNAERITMPPQVSLLFEVADLAVASPATVSRCGMVYNDYNDWGERDRPKEYGCYGRYDSTVVYVLFSLVCFVTVDEDGRLKMDAFVREKEACFPIKDTIFDYYVDAPNMNFSPWESKLTDDWKYDPESPFYKIIVPTIDTVRYEYVVTTLLGGECPVLLVGNVGTGKTSTAVSVTDSKDKSKYSVLNINISAQTTPQGLQDSIESKTEKRTKTTFVPIGGKKMIIFMDDFNMPAKDTYGSQPPLELIRQWIDYKFWYNRKNQQRTYVQNTLLMGGMGPPGGGRTVISPRTQSRFAVINLTFPPEVTITRIFGTMLKQKLQDFLPELVGLWKPITAASIDLYNARHFQGLPRNAKNTQRYQTTKASLIRVWIHEVFRVFADRLTNDSDMEWFLNALNTVLGRYLEVTFHSICKDKSVPIFGDFRNDKGYYEDLADLASLRQYMADMLVECNNTPGNPRMDLVFFKEAIEHVTRIARVISQPRGHMLNIGIGGSGRQVLARVAAHINEMGIFTIEVSKKYKAADFKEDLKTLYKMTGIKSRQMVFLFTGEQVAEESFMEILNNMLSTGEANLFKPEEFDELKTDLERPAKRHNIPLTTEALYSFFMNNVRDSMHIVLCLSPIGEKFRIYMRQYPALINATTTDWFRLWPQEALLEVAHRFLDDCELDKKVPGQKRGSTEDRDELVETKEDILHLAVANLVTGYKEFLDMKRHEISAAANKLRNGLSKIAETQEKVFSMSEELKVSQEQVNELAAQCEVFLMEIDAQSKEVEEAAEAVAIRSEVIAKEEVECLALAEVARADLELVMPMIDEAIRALDALSKKDISEVKSYAKPPLLVEKVMEGVMILIGKEPNWETSKKVLGEQNFLNDLKNFDRDHISDKTLKRIATYTGDARLEPQKVGVVSFACKSLMLWIRAIENYGKVYRIVAPKQARLNEALASLAEKQAMLAEAKRKLQELNDKLDKLRADFLEKSTMLEELKALAKLDKDYLNLPGDCLIATAFVSYFGPFDTKYREVLYEKWQNEILKKKIPCTEGLKMTMFLADPVTIREWNIQGLPNDEFSIENGVIVRLSTRWPLIIDPQCQAHFWIRNLEKKDLRIVDFGQLDYLKTFERCLQYGTPVLLQNVTESIDPAINPILRKTFTMQAGVKYIKFNDKYVPYKDSFKLYITTKLSNPHYPPEISAKTTLVNFAVKQSGLEAQLLGIIVRKEKPALEEQKDNLVMTIAKNKRTLMDLDNEILRLLNESKGDILEDDELFNTLQVSRATSIIVKESLSTAEVTEIEIDTARQAYEPAAERASILFFSIERSPKNPIVHDRILNINEYHTYSVYKNTCRGLFEKHKLLFSIHMCAKILQNSAKLVVDEYDFILKGGIVLDRQGQQPNPAPNWISEAAWDSITELDKIAGFHGIIDSFEQHSKLWHEWYATTNPETEDLIGEWNDKLNAFQKYCVVRCLRLDRMSYCMTMFVIEFLGPKFVEPPVLDLKSAFEESLPQTPLIFVLSPGVDPTASLINLAEQTKMSSRLSSLSLGQGQAPVATQMLTDGVKEGNWTGIKMTTEPPRGIKANMKRLYNKITDENFDKCDEPEKYKRLLYTLCFFHTLLLERKKFQQLGWNVEYSFNDSDFEVSEVLLALFLNEYEETPWGALKYLIAGVNYGGHVTDDWDRRLLMTYINSFFVDDAINVQKFRLSSLTTYYIPLDGNVQSYADHISLLPNFDKPEAFGQHPNADIASSIGEARNLFSTLVSMQDGVSSSVGESKEENVERLAKDIQSKVPELIDYEKTAKVIGLNRTPLEVVLLQEIERYNFLLNNMIVGLRDLRKGIKGLVVMSTELDDIFIAIFEGRTLNPPSLFWLAAYTFPTGFLTAVLQTTARSQKIPIDELSWEFYVFVEDDHAAARIMKESGVYVRSMFLEGGAWNRKQQCLQDPAPMELIAPMPVIHFKPVENLKKRTRGIYSCPAYYLPMRSGSYVIAVDLKSGSENSDYWVKRGTALLLSLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBC4</accession>
    <name>T1GBC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMN family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02005493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBC4"/>
    <dbReference type="STRING" id="36166.T1GBC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000555-RA">
        <property type="protein sequence ID" value="MESCA000555-PA"/>
        <property type="gene ID" value="MESCA000555"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801148_0_0_1"/>
    <dbReference type="OMA" id="AKWNEDG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20399">
        <property type="entry name" value="Tudor_SPF30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010304">
        <property type="entry name" value="SMN_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06003">
        <property type="entry name" value="SMN_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="6">
        <location>
            <begin position="73"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000555-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15294" checksum="6CFD2150B240E1E3" modified="2013-10-16" version="1">VEAALVTDPENTELLKLQKDLEEVIELTRDLIKTQLEEQKKSSYVEPATSAAYYDEIEAALIEAEKLVSPGRQWKVGDKCSAKWNEDGQYYDATIEGISASGEVSVVFDAYQNRSSTTIPELKERSASKTEVFPSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBI6</accession>
    <name>T1GBI6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02069662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000618-RA">
        <property type="protein sequence ID" value="MESCA000618-PA"/>
        <property type="gene ID" value="MESCA000618"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515227_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000618-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9562" checksum="6A994C4A594807B8" modified="2013-10-16" version="1">MEELSKREDLVLIVKYAQQRDSGIYECQVSTTPPVGYTMMLSVTGRDGNAMLTKQNRFDGSCSPCMYVVEKTRTIVEKTMTTVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBK8</accession>
    <name>T1GBK8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBK8"/>
    <dbReference type="STRING" id="36166.T1GBK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000642-RA">
        <property type="protein sequence ID" value="MESCA000642-PA"/>
        <property type="gene ID" value="MESCA000642"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796910_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000642-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7999" checksum="CFEE0FAFFF4EF3D0" modified="2013-10-16" version="1">MTDSIRRFQVLNSQILATLNKYLKSSDGEESNVKHVRCFLPPQHPTIMSSHYHEPNKLRQQQIQLTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBP8</accession>
    <name>T1GBP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorbitol dehydrogenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polyol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02182704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBP8"/>
    <dbReference type="STRING" id="36166.T1GBP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000685-RA">
        <property type="protein sequence ID" value="MESCA000685-PA"/>
        <property type="gene ID" value="MESCA000685"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_5_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="11">
        <location>
            <begin position="24"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="10">
        <location>
            <begin position="151"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000685-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="329" mass="34746" checksum="20B23C6D894E0047" modified="2013-10-16" version="1">NLVTCVFPTHISCLCTNLVGGGGGSDISILTKGRLGDYILDKPLITGHESSGVIVKVGKNVCNLATGDRVAIEPGVPCRYCTFCKAGRYNLCPDLRLCSIPPHAADFCHKLPDHVSLEEGALMEPLSVGVRACRRAGVSLGSKVLITGAGPVGLVTLLAAQAMGAANVLITDLAKHRLETAKLLGAHNTLEVDPKEDEKATVKKIHEIMGCAPDITIDCCGYEATTRLAILSTRSGGRVLVAGLASTDVKIPLVNALAREVDLIGTFTYGLEDYPASLALMASGKIKNINCLITHHFDLKDAPKAFETAQKGLDGAIKVMIHCQPRDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC72</accession>
    <name>T1GC72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF753 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099236">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC72"/>
    <dbReference type="STRING" id="36166.T1GC72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000873-RA">
        <property type="protein sequence ID" value="MESCA000873-PA"/>
        <property type="gene ID" value="MESCA000873"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109089_0_0_1"/>
    <dbReference type="OMA" id="GELCNNQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008472">
        <property type="entry name" value="DUF753"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721:SF26">
        <property type="entry name" value="DUF753 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721">
        <property type="entry name" value="GH09876P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05444">
        <property type="entry name" value="DUF753"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577253" description="DUF753 domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="2">
        <location>
            <begin position="30"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="2">
        <location>
            <begin position="107"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000873-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="22601" checksum="08435FFA9563E5B1" modified="2013-10-16" version="1">MNGKVLVVLAVLTAVLAIDNIYDPSKPRLCYSCSGSNCQRTTTSSVNTTCADNLDICVTVFDGFEVTARGCLSELPDSQRSKCNSKSIECDKCLSDLCNKFGREDFKCIDCDSSKNAKCKDDVSNIVPTRCPVPTSAASYCYVDRRGGNIVRGCSVDTVHQRGCLEDQQCALCLPEDGEACNGKFNVNGSGAIQVSLIALFSAFIVAYNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDC0</accession>
    <name>T1GDC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDC0"/>
    <dbReference type="STRING" id="36166.T1GDC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001304-RA">
        <property type="protein sequence ID" value="MESCA001304-PA"/>
        <property type="gene ID" value="MESCA001304"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070940_2_0_1"/>
    <dbReference type="OMA" id="EIVNKAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042858">
        <property type="entry name" value="DNAJC8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15606:SF4">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15606">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 8/LIPOPOLYSACCHARIDE SPECIFIC RESPONSE-7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001304-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="28670" checksum="6195C56B875DBD64" modified="2013-10-16" version="1">MSGKEGTSSKTESFNDFYTEVKQIEKRDSVLTSKQQIERLLRPGSTYFNLNPFEVLQLEMTHHWTKSRKNTNPDDRDRAQTAFEAVNRAWKVLENDVTRKRCLEMYEEAKERTDHMIAEKRKKLKKEGKTNEKIEEDDPEKYKHSCYVLVMKLFADMERKRQSLEQRDMEERKRKREAEIDEEEKRKNAKEWQKNFEESRQSRVDSWHTFQSGGAKKKKKEKKNKAGMFVPPKLKPESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDF0</accession>
    <name>T1GDF0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001335-RA">
        <property type="protein sequence ID" value="MESCA001335-PA"/>
        <property type="gene ID" value="MESCA001335"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888339_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7227" checksum="1A423EB623C3DDED" modified="2013-10-16" version="1">MLSTPKHIAIEPIITMGSYCENREKKVQPRAETTAPKPRRCPKDQSRSFATTAAEHACWWRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE44</accession>
    <name>T1GE44_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cholesterol side-chain cleavage enzyme, mitochondrial</fullName>
            <ecNumber evidence="5">1.14.15.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">CYPXIA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Cholesterol desmolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Cytochrome P450 11A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Cytochrome P450(scc)</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; C21-steroid hormone metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.6" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02000306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE44"/>
    <dbReference type="STRING" id="36166.T1GE44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001602-RA">
        <property type="protein sequence ID" value="MESCA001602-PA"/>
        <property type="gene ID" value="MESCA001602"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877789_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008386">
        <property type="term" value="F:cholesterol monooxygenase (side-chain-cleaving) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF3">
        <property type="entry name" value="CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="25" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="20" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576844" description="Cholesterol side-chain cleavage enzyme, mitochondrial" evidence="25">
        <location>
            <begin position="22"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00033274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00033394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001602-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15649" checksum="F5F8D2D9D987143C" modified="2013-10-16" version="1">MVNDLQTSWFSLVLLSLYSSGRDENHFPDPEKVIPERWIRNNESGELDLVYKAHGTLPFALGGRSCIGRKIAVSETPPAAFLQFQVLQRHLWVDIAHPVKKSINQFKFLSEREKLFGVKSFMRKPLWFLCNINSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE85</accession>
    <name>T1GE85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02027893">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE85"/>
    <dbReference type="STRING" id="36166.T1GE85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001643-RA">
        <property type="protein sequence ID" value="MESCA001643-PA"/>
        <property type="gene ID" value="MESCA001643"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_640264_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF575">
        <property type="entry name" value="LOCOMOTION-RELATED PROTEIN HIKARU GENKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="139"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="207"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001643-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="429" mass="48566" checksum="6B5C455CCE25C301" modified="2013-10-16" version="1">EINCGHPGVLYNGWLENLEGGTGLGASIIFRCQPEMMLIGNSSTVCQIDGRWRYPAPECLAPCIVPTISQGHVIPIELITDDNTTTVTTPAVITSVGKVKHGTALEVNCDQYYEFPISSLSPPTCNNGTWSTIPRCIPARCKNMPRAPKHGMVLAPKTEHGMKARFKCKDGWKLTDRSGKEITDSEQYVLTCSFGNWTGETPYCHEVYCSFPGFVENGKVLLVGNMGLYDYRPYVKKIVNNKQIMYECDKGYVMEEGGAPGATCIGGRWRPTQVPKCNLGQHPRLRWSRKRRSLEMRYMKSKLFFDPQMELIRLSLIHTHFCKCEIRKLFPFYFILNRCVDIGKFEMIGSNERTCIHSEWTGVKQFVMVLTKRMTMLDGEWIGRAYACENGTCSFVNDEPNVVSFYNDLEIRESTVEFPPGATIISRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEB2</accession>
    <name>T1GEB2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001671-RA">
        <property type="protein sequence ID" value="MESCA001671-PA"/>
        <property type="gene ID" value="MESCA001671"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888153_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001671-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15697" checksum="145E4E5D8A26108D" modified="2013-10-16" version="1">MSGSALDLPFSFLSFIRSCVAPGFHMVLGFQKNFTFESLKFAFGRIMPSLNLIGRRFRIPDKLSINKFKKFYKANFIKLEERSQALCQSLGAYQRASLYNPYKSLIWAHAPTEDREDEAKDNFNEQFEQAFFPMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GET0</accession>
    <name>T1GET0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GET0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001851-RA">
        <property type="protein sequence ID" value="MESCA001851-PA"/>
        <property type="gene ID" value="MESCA001851"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770187_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001851-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16812" checksum="4D22F599CA5B5E46" modified="2013-10-16" version="1">MKKEISYITGCIPQSQSRINFVKTISIAQYVARFHANNVADVDIGHFHYHFKQHVATPLIVGKELECCHLFEEFSGTCFSVIFLNSYKPPTTTRTYLKAFHEEDFIGGSHIFTCTSPRKHLALDPKILKGKCNIFPNKQVTQTYGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF01</accession>
    <name>T1GF01_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001929-RA">
        <property type="protein sequence ID" value="MESCA001929-PA"/>
        <property type="gene ID" value="MESCA001929"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239691_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001929-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12027" checksum="6BAC9D26BE56EE1D" modified="2013-10-16" version="1">MANEASVPTIINKSYGSWIESVEKSGFTYNLGVNIKFAKVNKFLEEFLQFRGGSKQDMWDSSKCVQDLITFKEFNAWMGIAKYLVNYLGAHLETRLRVQDMSLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF78</accession>
    <name>T1GF78_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="8"/>
    <dbReference type="EMBL" id="CAQQ02104087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF78"/>
    <dbReference type="STRING" id="36166.T1GF78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002009-RA">
        <property type="protein sequence ID" value="MESCA002009-PA"/>
        <property type="gene ID" value="MESCA002009"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_3_2_1"/>
    <dbReference type="OMA" id="WARRPAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF153">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase C-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002009-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15441" checksum="7392B0E459F6B5EA" modified="2013-10-16" version="1">MTEEKAKQLGFKPKAYLRDFLYVSQDPIDQLLLSPAYAIPKLLKKTGLTLKDIDSWEIHEAFAGQIIANLKALDSDYFCQKHMGLSEKVGLPDMNKWNNWGGSLSIGHPFAATGVRLCMHTVSQGVAMLIERYPGATAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFE1</accession>
    <name>T1GFE1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dehydrogenase/reductase SDR family member 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02091003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFE1"/>
    <dbReference type="STRING" id="36166.T1GFE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002082-RA">
        <property type="protein sequence ID" value="MESCA002082-PA"/>
        <property type="gene ID" value="MESCA002082"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640954_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8294" checksum="77B1ECC885CA3273" modified="2013-10-16" version="1">MDRWKNKVAVVTGASSGIGAACVVGLVKAGMVVVALARREDKLISLKQSVPQNLQSKIDRNGDQSLAVGSYYERYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFV8</accession>
    <name>T1GFV8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002259-RA">
        <property type="protein sequence ID" value="MESCA002259-PA"/>
        <property type="gene ID" value="MESCA002259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1469849_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="184" mass="21269" checksum="5BAAC39D9362FA86" modified="2013-10-16" version="1">MLISKVYKLSIHYDFKIVHCKMQSLIVIFLSIFLFCGVYGSSEIDSINQKISDLKKCCTVEIVKTERSPRTKQISKECLEEDYVKSAGDNQQLCLVYCINIKIGAYNPDSGFNNALYSSKLKSLIIKDAIWKENVDSVQIVDKCFKNVVPNIEKCSIEFLKSRYCFWKEIIKYCPADLRNHDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG22</accession>
    <name>T1GG22_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002327-RA">
        <property type="protein sequence ID" value="MESCA002327-PA"/>
        <property type="gene ID" value="MESCA002327"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349078_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002327-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11179" checksum="C1CCAD01655FFCBF" modified="2013-10-16" version="1">MSDQKKVYVRKEEKNFLSPFYPTEIVLKYNGSKLIVIVPGAENRLYMQVSDTSFFPKTISFRTSKENIVRVFYDCPMGTDAFQDDEISGFAFPETVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGH2</accession>
    <name>T1GGH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02010149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002492-RA">
        <property type="protein sequence ID" value="MESCA002492-PA"/>
        <property type="gene ID" value="MESCA002492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530042_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="10001" checksum="E7F3E40B5F127D24" modified="2013-10-16" version="1">MYEVIKNNKSTGTDDIFYRLLTKIWISEKISNEWKVSFICPLNKKDYRSDCKNYRCMTLLSVAYNILPCECNCKRISTRQQVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGI8</accession>
    <name>T1GGI8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGI8"/>
    <dbReference type="STRING" id="36166.T1GGI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002508-RA">
        <property type="protein sequence ID" value="MESCA002508-PA"/>
        <property type="gene ID" value="MESCA002508"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_6_1"/>
    <dbReference type="OMA" id="ANDWPEA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000256">
        <property type="entry name" value="cyclin-dependent kinase 10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF508">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="64"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002508-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="25897" checksum="02D82002A66B0BE3" modified="2013-10-16" version="1">MQDSSKLYLLRNFENMAQNESLKNVFDPQAPVTRKGVLMNFKTGDPMCIPDKDMFGRCRQVTEFEKLNRVGEGTYGIVYRAKDTKSGEIVALKKVRMDQEKDGIPVSSLREIMILKSCNHENILKIKEVVVGKSLESIYLSMEYCEQDLAHLLDNMQNPFSESEVKCIILQVLKGLKYLHSNNILHRDLKVSNLLMTDTGRVKIADFGLARYFGYPPKPMTPNVVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGU8</accession>
    <name>T1GGU8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002629-RA">
        <property type="protein sequence ID" value="MESCA002629-PA"/>
        <property type="gene ID" value="MESCA002629"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1290295_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588287" evidence="1">
        <location>
            <begin position="17"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002629-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="214" mass="24931" checksum="2D980C2C5BBA30B8" modified="2013-10-16" version="1">MKLFVFAVFLVASVVADVSHLDLGYDYQPGHPSFDEGPNNEYIPPAIESKVIHNEPVVHHHEPVIHQQEVHREVVHHEPVVHHHAPVVHHEPVVHRQEVHREVVHHEPVVHHHEPVVHHQEVHREVVHHEPIVHHAPVIHHQPEVHREVVHHEPIVHHQREIVHHEPVVHHEPVVHHEVDNTYIPPASDPLPAHVLNDDGYVYKNPGNFRAFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHD7</accession>
    <name>T1GHD7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002832-RA">
        <property type="protein sequence ID" value="MESCA002832-PA"/>
        <property type="gene ID" value="MESCA002832"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751808_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002832-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17000" checksum="302F54B98F46BB2C" modified="2013-10-16" version="1">MCFWHLFRSFREKKPTVKKNDKKKRLDKKILSKSNELNAISGEPRLTHLSKQRPKRNKKCQSLVVSTHESSEEILEGFDVFFGSDLKFPQTSIGFGNKTNSILYESIKECECESMNCEPPSLPANRDSVKGSPKEPESVIYENFNVVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHS1</accession>
    <name>T1GHS1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002979-RA">
        <property type="protein sequence ID" value="MESCA002979-PA"/>
        <property type="gene ID" value="MESCA002979"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888345_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002979-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7494" checksum="FCC6697F5022AB0E" modified="2013-10-16" version="1">MGQAKYLVNYLLRQQNPPMILKPNRYFGPKIPMIIINPLRTANRPHCPIWIKFSQEPLIIHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIQ0</accession>
    <name>T1GIQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stathmin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIQ0"/>
    <dbReference type="STRING" id="36166.T1GIQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003335-RA">
        <property type="protein sequence ID" value="MESCA003335-PA"/>
        <property type="gene ID" value="MESCA003335"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657310_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="9031" checksum="B054EAC2D48E7FB0" modified="2013-10-16" version="1">MVVHTAEIEKTRQTLEQQKEMEKQAIEEKLKNAQQSRDDHIKKMLARLKQHEKQIEAVRQKKYSVENNSYPIPVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIQ2</accession>
    <name>T1GIQ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIQ2"/>
    <dbReference type="STRING" id="36166.T1GIQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003337-RA">
        <property type="protein sequence ID" value="MESCA003337-PA"/>
        <property type="gene ID" value="MESCA003337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029352_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="14299" checksum="1E882704D197FD24" modified="2013-10-16" version="1">MDAFRTIGYINSSQPVITRNPRVSVTHDKHDRHRTWYLHINNVHEEDKGRYMCQINTVTAKTQFGYLNVVGKDNHVSLTSECSKNPFLKLPRKHTQVCFLFVVQVTFVNILKYAHKKLSRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ99</accession>
    <name>T1GJ99_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ99"/>
    <dbReference type="STRING" id="36166.T1GJ99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003543-RA">
        <property type="protein sequence ID" value="MESCA003543-PA"/>
        <property type="gene ID" value="MESCA003543"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388731_0_0_1"/>
    <dbReference type="OMA" id="CGISKTN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003543-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10605" checksum="99CF8C974E2EAE0C" modified="2013-10-16" version="1">MSVQIEQVKNFIDKSLQDKSKPWFQAFEFLEKKTTINRTHLFAGAAALTALYLVFGYGAQLLCNVIGVVYPAYISIHAIESSTKVDDTKWLIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJG0</accession>
    <name>T1GJG0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJG0"/>
    <dbReference type="STRING" id="36166.T1GJG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003606-RA">
        <property type="protein sequence ID" value="MESCA003606-PA"/>
        <property type="gene ID" value="MESCA003606"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500490_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003606-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9671" checksum="1764F5ACA621FD68" modified="2013-10-16" version="1">MKADDGNFQRSLLLTMYLPQMITKRLVVFSTDQLIVAFGGAASFFLGCNFLSVIELLYFVLHFCWEKCKKVAMAASAAVDLQQKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJT5</accession>
    <name>T1GJT5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02027138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003739-RA">
        <property type="protein sequence ID" value="MESCA003739-PA"/>
        <property type="gene ID" value="MESCA003739"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154166_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003739-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="241" mass="28681" checksum="61D800E6C0A7348A" modified="2013-10-16" version="1">YGSGPLYNKYNNQIKFYRNKLGNEFGYRNKIKPKGGKEGMEQLEDIKQEILTTTNEDEEIISKLKEFFPPYNVFINSWDKVYHIRNQDYNLEFKDIISKWPVFENTDICHHLISHDFKRKFPNIPQDFSSKWSHLEKILINHEKAKKLDILPNDWDTQIRIFQLVHHLSPPVIYQVHYSDNGEGLTKPKWKPHSDKKQKLKFSVLESIKSMIMVLEHGENFKALVKDFAAEKHHSGFHFHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK08</accession>
    <name>T1GK08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02106642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK08"/>
    <dbReference type="STRING" id="36166.T1GK08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003819-RA">
        <property type="protein sequence ID" value="MESCA003819-PA"/>
        <property type="gene ID" value="MESCA003819"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256781_0_0_1"/>
    <dbReference type="OMA" id="VFLCKLM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF22">
        <property type="entry name" value="GH21596P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="3"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003819-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11913" checksum="AA8AE0E6D1BD4643" modified="2013-10-16" version="1">RNKDQEAEVLNWIFEVLGEKVPSGQYEDILKDGVILCKLINKMAPGSVKKIQERGTNFQLMENVQRFQAAIKKYGTQKHPEYTGPSLGPKMADKNERTFTEEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK90</accession>
    <name>T1GK90_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Innexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003909-RA">
        <property type="protein sequence ID" value="MESCA003909-PA"/>
        <property type="gene ID" value="MESCA003909"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2678096_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003909-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8648" checksum="9DA2BD320DDCFF60" modified="2013-10-16" version="1">FVLTNSYTKFIILWYVYCVAALNAIVERIVLLMAIIGTTFAQSNTYLPPKQNGYDYPAPPGRPVRCYYKYIFILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKV6</accession>
    <name>T1GKV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AF4/FMR2 family member lilli</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Protein lilliputian</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Has a role in transcriptional regulation. Acts in parallel with the Ras/MAPK and the PI3K/PKB pathways in the control of cell identity and cellular growth. Essential for regulation of the cytoskeleton and cell growth but not for cell proliferation or growth rate. Required specifically for the microtubule-based basal transport of lipid droplets. Plays a partially redundant function downstream of Raf in cell fate specification in the developing eye. Pair-rule protein that regulates embryonic cellularization, gastrulation and segmentation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02109601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02109602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKV6"/>
    <dbReference type="STRING" id="36166.T1GKV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004137-RA">
        <property type="protein sequence ID" value="MESCA004137-PA"/>
        <property type="gene ID" value="MESCA004137"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_274027_0_0_1"/>
    <dbReference type="OMA" id="NMEATWT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032783">
        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007366">
        <property type="term" value="P:periodic partitioning by pair rule gene"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007797">
        <property type="entry name" value="AF4/FMR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043640">
        <property type="entry name" value="AF4/FMR2_CHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528">
        <property type="entry name" value="AF4/FMR2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528:SF17">
        <property type="entry name" value="AF4_FMR2 FAMILY MEMBER LILLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18876">
        <property type="entry name" value="AFF4_CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0562">Pair-rule protein</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AF4/FMR2 C-terminal homology" evidence="14">
        <location>
            <begin position="1076"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="230"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="306"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="682"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="862"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="947"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="327"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="455"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="512"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="552"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="579"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="604"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="620"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="682"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="734"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="751"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004137-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1172" mass="127070" checksum="C0F7E0B4697FCF5B" modified="2013-10-16" version="1">MEEYERRQRRERDKLARQGKSNQNPFNESTLFFEPYRVVEEEEHQKISEKLDSQKTSSTSSSATYNGRGGGGYPGPGPTVKNDSIHSKSGGVATVKPLMPPVPHSPTLPTAAATVAATAPPNGARFSQSFKRKSENQGSSSDVSSILSQMTKTIVPSNNLLTEISATPRNPTPFQNPEQQNRHKYSFDIKQLTVPSNIPPLVSPFLDEPPTLSPLVAPINAVPPITKSTDLEKDLQVSDSDDEKLQKTASVDQMSNNGGSESSDSSGSSDSESEASNENGNKLPHPAVVSSNRTIHLQAAVSLANNENSLQIKNEPTNDESSRSPLVKKEEITIKAERLDSPNVISPLAPPPEEPPAIGDGVNFPVLSDLSSNGSISSAEEDPAPKLPPPQPPVKSSPAKKVTTPSKDTTERKKRRKKVDKSSNVDSSDEEEVDSNAMVSAIPDKKQKRGRPRKTSGSVSSSTSSSLKTSAAQKKKNKDSKKKSKKPVKRRKNSANTSSLSSTSSSEDEEATAPAPAPTQTPTQNQHLPPQPPPPVQSNVPVVETIPSAQKSSSSSSSSSSSSSSDSSENELVCEAEVSKSPTKSVKSKKMLSSCSDSSSSDNEENKKIDQEVKSDHNKGKIFSRVYKKGGAKNKGGQVVIVDSAEEVQQKPPTIQAVPPQPVTQPPQIPLQKIMSPITAFTSSGFTPNHLPKSSSKSPRVVNMKTPEHGATPTHSSMNNGKVHVIGGGSGSSRTPNRTPTRTPTRTPTPVNSAQKQSPSPVRQPASRTSSVSPHPNNLNATATVASTTTTMTTTVVNVAQGMPSLICKIDLGRLSNVPQEWYQNSSSLSSNALSRQYTSPRTLPVNRTPPVTVLEERLITNSMKGSGAAGTGSGSTTPRESSNLDCLVESISSPTLIKPIKMETNSNATTYMFEYSDYNAESFDNFKKMANNNNNTVKQEQFIKNEIKQTPPPMSSELSPPVGKDGDKVNSRKRSSSGSRSPYKDKKRKKDKGREGNNGAGNNGFASKELNQHLPPTNHERLSNSINERTTRSPIPPAHQNHQPMSPIPNELPSITPQPIIKKVYKSYYNRDDIVKIKQEVGDQKTVLDEAVRLKHEADMEKVPFKQVLKYLDAVKYFLLTGSFMEETKLDLKPAFNMYKETLHLIRFISSKFNHEQDNVNHRIVNVLSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLI5</accession>
    <name>T1GLI5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02099123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GLI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004388-RA">
        <property type="protein sequence ID" value="MESCA004388-PA"/>
        <property type="gene ID" value="MESCA004388"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515230_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055120">
        <property type="term" value="C:striated muscle dense body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043266">
        <property type="term" value="P:regulation of potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051951">
        <property type="entry name" value="UNC-93_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444:SF50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444">
        <property type="entry name" value="UNC-93 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004388-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9488" checksum="A39A676F044EDDAC" modified="2013-10-16" version="1">MLAVTLKLLKERYQLLLLPITMFIGAEEAFVAVDFTASFVACGWGISRIGFAMICYGVFNALASXXXXXXXXXXNLMTLRFTLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLN9</accession>
    <name>T1GLN9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004452-RA">
        <property type="protein sequence ID" value="MESCA004452-PA"/>
        <property type="gene ID" value="MESCA004452"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963455_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6757" checksum="F5538612A8D1C6CC" modified="2013-10-16" version="1">MPAAGMLLTITHLAQMSTWVRLDIGQQHQRRSNFRERSRLDSTTSSYFQCLFTALRVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLZ3</accession>
    <name>T1GLZ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004559-RA">
        <property type="protein sequence ID" value="MESCA004559-PA"/>
        <property type="gene ID" value="MESCA004559"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2099658_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13537" checksum="4F076DCF7D47BF16" modified="2013-10-16" version="1">MATWLKQDAKALQVIVTTITEEHKLHITNCDDAHEMWQKLESIYEMKSESHFYLSWKSTPKTERTLRNLRSRLCMNEARLGIKLIEYSGAVASFVRSSAEDEDKLFEPRYADGLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM80</accession>
    <name>T1GM80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004648-RA">
        <property type="protein sequence ID" value="MESCA004648-PA"/>
        <property type="gene ID" value="MESCA004648"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004648-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9573" checksum="949A9E8C367ED260" modified="2013-10-16" version="1">MKECWHHNANVRLPILNVRKKIKKIIDETDRMKTIEEEDEDKKLSNSINNCSTSEEKSSSLSEDRSNDKKNENKNLSFFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMF4</accession>
    <name>T1GMF4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004728-RA">
        <property type="protein sequence ID" value="MESCA004728-PA"/>
        <property type="gene ID" value="MESCA004728"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3430071_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="19" mass="2270" checksum="956FD830845143A0" modified="2013-10-16" version="1">MPSFQILMKISKDLWFGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
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    <name>T1GMG7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L35</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02074306">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMG7"/>
    <dbReference type="STRING" id="36166.T1GMG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004741-RA">
        <property type="protein sequence ID" value="MESCA004741-PA"/>
        <property type="gene ID" value="MESCA004741"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110381_2_1_1"/>
    <dbReference type="OMA" id="HIVMNQQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004741-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14448" checksum="FA42184AAAB107E1" modified="2013-10-16" version="1">MVKVKCSDLRTKDKKELTKQLQELRTELLSLRVAKVTGGAPSKLSKIRVVRKAIARVHIVMNQQQKSNLRKLYMSKKYKPLDLRRKKTRAMRKALSTKDANRKTLKEIRKRSIYPQRKYALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMW8</accession>
    <name>T1GMW8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMW8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004902-RA">
        <property type="protein sequence ID" value="MESCA004902-PA"/>
        <property type="gene ID" value="MESCA004902"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203574_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004902-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12254" checksum="C7271AB48550B044" modified="2013-10-16" version="1">NALLSQQQQKFSFGLGKTPNFYLKHRKNAILETKEETQNIMATLEKPKSKKQKKKVVGEDFEGITPEPEPEYSSSTAACSKLVSYECAVSKEFHTVKEEDFEEETPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN81</accession>
    <name>T1GN81_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005025-RA">
        <property type="protein sequence ID" value="MESCA005025-PA"/>
        <property type="gene ID" value="MESCA005025"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624812_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005025-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9054" checksum="501E03D0309A0BFF" modified="2013-10-16" version="1">MLDLKSKFMPILEKVQQTNDTLETTSRIMNKAYNILEEFLNNVPRSSLIKDVFKKIEDTLNASQYSIDFGEIQENPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GND4</accession>
    <name>T1GND4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02138495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GND4"/>
    <dbReference type="STRING" id="36166.T1GND4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005081-RA">
        <property type="protein sequence ID" value="MESCA005081-PA"/>
        <property type="gene ID" value="MESCA005081"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075836_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF10">
        <property type="entry name" value="TRANSCRIPTION FACTOR GAGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="2"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005081-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13099" checksum="0DAB52AFC5D803E8" modified="2013-10-16" version="1">MLAGVNPTDLEALLEFVYRGEVSVDHSQLPSLLQAAHCLNIQGLAPQTISKDEYANTQYQLPLMQQHGETELITALNTSQHTQQQLHTQVIENITADHHTLTSTNANPPQNTMKSEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPN4</accession>
    <name>T1GPN4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPN4"/>
    <dbReference type="STRING" id="36166.T1GPN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005570-RA">
        <property type="protein sequence ID" value="MESCA005570-PA"/>
        <property type="gene ID" value="MESCA005570"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880593_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF16">
        <property type="entry name" value="C-MYC PROMOTER-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005570-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15396" checksum="EBA0064B3A5FF455" modified="2013-10-16" version="1">MFPAPLLDATQQSLILILQPDLAEDDLAFKHSSSNTINSSTGTISGNFVEKEDDDEMKDKQIRAVFMRLFAQLLQGYRSCLTIIRIHPKPVITFNKSGFLGAKDLIENEFISRVLESMFFTGFVNERGPPWRQIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQE4</accession>
    <name>T1GQE4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02096522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQE4"/>
    <dbReference type="STRING" id="36166.T1GQE4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005848-RA">
        <property type="protein sequence ID" value="MESCA005848-PA"/>
        <property type="gene ID" value="MESCA005848"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582574_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005848-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="18378" checksum="3D08BC37B778455D" modified="2013-10-16" version="1">MITGLDPAAPLFYKLPETHRLSMNDATCVETLHTTQGAGFPCELGRVSYFVNGGTVQPGCVPSYNLISVETCSHSIAHNYYAEAIRLGNDNLFYSEKCENRNCCQEKADVVQKPGTNSTCKNSGIFAFNTHPYGLNATEDYGLGTDGAVSCSGKECYQNSIFYPCKGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQE8</accession>
    <name>T1GQE8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021459">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021461">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005852-RA">
        <property type="protein sequence ID" value="MESCA005852-PA"/>
        <property type="gene ID" value="MESCA005852"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064136_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005852-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="14436" checksum="565F1B85DDB83AFF" modified="2013-10-16" version="1">MLLFVQHYLATCPFYATFTVKSPKNYHFCVFMCLFIEEYYKMNDHRLGNVNLQHQQHLRNHRICEKLEKGKLLITKRIITRNTIHQNVYNYLEKRMECLNNNCFWLYTSDVCSFIADNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRA2</accession>
    <name>T1GRA2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Protein-tyrosine sulfotransferase</fullName>
            <ecNumber evidence="2 5">2.8.2.20</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16801"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:11688"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:65286"/>
            <dbReference type="EC" id="2.8.2.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the protein sulfotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.20" evidence="2 5"/>
    <dbReference type="EMBL" id="CAQQ02136517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRA2"/>
    <dbReference type="STRING" id="36166.T1GRA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006180-RA">
        <property type="protein sequence ID" value="MESCA006180-PA"/>
        <property type="gene ID" value="MESCA006180"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590543_0_0_1"/>
    <dbReference type="OMA" id="SHWIKSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788:SF10">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006180-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19115" checksum="15A9ADC810E80FA7" modified="2013-10-16" version="1">MPLIFIGGVPRSGTTLMRAMLDAHPEVRCGQETRVIPRILQLRSHWIKSEKESLRLEEAGISKEVMNSAIAQFCLEIIAKHGDPAPRLCNKDPLTLKMGSYVIELFPNAKFLFMVRDGRATVHSIISRKVTITGFDLTNYRQCMTKWNHAIETMHGQCKEIGKERCMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRZ4</accession>
    <name>T1GRZ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006441-RA">
        <property type="protein sequence ID" value="MESCA006441-PA"/>
        <property type="gene ID" value="MESCA006441"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925266_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006441-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6617" checksum="1F9EAD9A38D5E578" modified="2013-10-16" version="1">MCFPCFVGSLKLKFLAGLDTGYNASENETNCELNLLSVIGSLSSSHRAKPFFDEPPSCTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS10</accession>
    <name>T1GS10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006457-RA">
        <property type="protein sequence ID" value="MESCA006457-PA"/>
        <property type="gene ID" value="MESCA006457"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608777_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006457-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9029" checksum="D46E6E2626D5D5D4" modified="2013-10-16" version="1">MVTKFQATSFLKKCRLARPYNSSTYLHPHHHIRLYTLDYSIALCYCNCSYILVYKFSGSGSSSNSIPTFDNYTGICLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSH2</accession>
    <name>T1GSH2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GSH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006630-RA">
        <property type="protein sequence ID" value="MESCA006630-PA"/>
        <property type="gene ID" value="MESCA006630"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418434_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066:SF2">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006630-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22218" checksum="471344D7E9FEBD73" modified="2013-10-16" version="1">QWNNHGYDVAKIFGKKFEQISPVWLQIIPNGDNNYKITGLHDVDSGWMKDVKKAGVKTKILPRVLFDKFNEKDYSKMLTFDKDRRLINELLVKICKEHRFDGIVLEVWSILAARVDDDILYRLIIDMAEHLKSEKLEFVLVVPPYRDDMHELFRGGGGGGXXXXXXXXXXXXXXXXXXXXXGICHRVFFDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSR5</accession>
    <name>T1GSR5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSR5"/>
    <dbReference type="STRING" id="36166.T1GSR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006726-RA">
        <property type="protein sequence ID" value="MESCA006726-PA"/>
        <property type="gene ID" value="MESCA006726"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_3_1_1"/>
    <dbReference type="OMA" id="KNTEPDA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF241">
        <property type="entry name" value="CUTICULAR PROTEIN 47EG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577597" evidence="3">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006726-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="13936" checksum="8A04B21FC80E5407" modified="2013-10-16" version="1">MKLFVVSALILFGFTSTLVFSAPTSSKTDDSMISLVRSEQSVQPDGYKYSYELDNGQKAEETGHFTDDEEFLIHGSFEYTSPEGDKIELQYTADKNGFQPQGAHLPTPPPIPEIILRAVEYLTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSV2</accession>
    <name>T1GSV2_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006764-RA">
        <property type="protein sequence ID" value="MESCA006764-PA"/>
        <property type="gene ID" value="MESCA006764"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798643_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577103" evidence="1">
        <location>
            <begin position="20"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006764-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="17354" checksum="552D2F68F6AB4DE0" modified="2013-10-16" version="1">MCFFALKLLIICIFSIVSSKCEFKHPSCDFDLVEIRTSILEYLPKECQELYKEDQTDGIEKFKKAKEESLVFKKFYEALKSKTETFDENDPENLEINFHLLKEIYTKNQNEFYFNRCKLQKDFMNYFECLMEERKEIEGKLNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTI9</accession>
    <name>T1GTI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTI9"/>
    <dbReference type="STRING" id="36166.T1GTI9"/>
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        <property type="gene ID" value="MESCA007022"/>
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    <dbReference type="OMA" id="CENNCRD"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="108"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
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            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007022-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="370" mass="43890" checksum="F0BB79E06657EE3A" modified="2013-10-16" version="1">MDDLDIYGDLDVDEIYVEKNKERENARINELESEVRKHRDDIEKLKKDNERLNKKVKIMETNFNNLFDVVKSEISRKDAQIHALRKEKDNLIFRRNVSKENSIVNENINEKALSKSDRTGKRDERDHRKRDEKDDQGSKDNREFKDYRRDRKRSRSRDRKRRRSKSPKTNSPERKSRDFQNQSPSTSRKSSEKEDGNDIEHELLEELRQREKLIAQIEKYSKELELSINSHDFDAQRQAILCQLKEEDESLQEPPSKNGYIFNEHFTQMDNRVFTSVPIENVPLNAKIEVEDRVEYNNNINIVKSNYNELNNENPSDPKQNPFQNKAMRKEYTTVLTANETLQLCGFGVAQNEPTTTLKITGYQEFKKNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTS1</accession>
    <name>T1GTS1_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1GTS1"/>
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        <property type="gene ID" value="MESCA007112"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778751_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007112-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7522" checksum="4FC8B2746802C568" modified="2013-10-16" version="1">MLPRSPINHDTPGGFSTSTAMSPSFSPSLSPLATGSPSMTPHVHHMVAMQRQPQPSHRKSLDTDMRVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUA4</accession>
    <name>T1GUA4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119790">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1GUA4"/>
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        <property type="gene ID" value="MESCA007314"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RT03134P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12335">
        <property type="entry name" value="TIPE PROTEIN TEMPERATURE-INDUCED PARALYTIC E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007314-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="21207" checksum="0CC3BBC11EF3A92B" modified="2013-10-16" version="1">MPSTRVFKSGIDETPVMCTTTRAVTSDNCEWGSCGEWCLSKTSGSCIQIFVNLRTNGSDLAFQNCTNSANKTCYGIDQEHADKARCINDECKNLTGTFNCTAGQCLNITDAFECMFINSDPPVKCSGRRGKINCKDIAGLYSCVRGTCRKIRTPYNCDRRCVDIPTRNKNVVILSGDKVFLSQCENAVDLETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUP8</accession>
    <name>T1GUP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Single domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394713">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007466-RA">
        <property type="protein sequence ID" value="MESCA007466-PA"/>
        <property type="gene ID" value="MESCA007466"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158197_0_0_1"/>
    <dbReference type="OMA" id="IGYCGRH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029277">
        <property type="entry name" value="SVWC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053308">
        <property type="entry name" value="Vago-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957:SF2">
        <property type="entry name" value="GEO11553P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15430">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01318">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588522" description="Single domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Single" evidence="4">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007466-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13637" checksum="EA31B2528CD27AA0" modified="2013-10-16" version="1">MKVFFVLSLVLGTIYASGVIYRGNAKHPDFPNQCYYKDGDKALNFDEVYRPIGECLKVKCRDDYVLGINYCSRYHVAPKCEEIKEDLMKPFPECCPKIKCLDKNGTEIIRDTTYDSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUQ1</accession>
    <name>T1GUQ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007469-RA">
        <property type="protein sequence ID" value="MESCA007469-PA"/>
        <property type="gene ID" value="MESCA007469"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1284601_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007469-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="24033" checksum="D4E34615F47DAA98" modified="2013-10-16" version="1">MKLETSIADIRPGDGILAAVIEEVLVESTIEVFENTEDYKTFKLIESRPSISEIQKTHLRGPVTEEVLAFESSVDLRKIGPEEPTKLSPVVDSSLVAPSKSEISPIESTGTYDSKNIIQFKTTTLLEPALSASNTTVLFEEFKEVKPKELIEGDVKLYSHEQKVTIIQSDTPEILEHQTQTQSTTIDKTQTQPQIEMQLKPKKTKTFTKIVTTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUY3</accession>
    <name>T1GUY3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007555-RA">
        <property type="protein sequence ID" value="MESCA007555-PA"/>
        <property type="gene ID" value="MESCA007555"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592509_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007555-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8989" checksum="E7F5E42491D81ADC" modified="2013-10-16" version="1">MNKLEASILDTSAIHTNKVEIICDDKVNSSRLVLTNFGNNSFASLIVHNLSDDFCAFWDSKLINSVNRSSIHDEQNSRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVI7</accession>
    <name>T1GVI7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GVI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007793-RA">
        <property type="protein sequence ID" value="MESCA007793-PA"/>
        <property type="gene ID" value="MESCA007793"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1373642_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007793-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="23315" checksum="E8BF34FFADB05F12" modified="2013-10-16" version="1">MVRTAATEILGIQKHRKTHGWHDEEYDRAFGEKKLSIKQATKRKPDNFEEMEIPGNRHRGEEEFFFEILHNNEXXXXXXENRHRGEEEFFFEILHNNEEETLPTRAEVDRAMKNNKSACTDCIHAELFKAAGSGFNNELHQILLSIWNSEEMHEKWNNSTICPIYKIVSFLLDRTDGDCKNYRGISLLNKKKTKSSHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVL1</accession>
    <name>T1GVL1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007821-RA">
        <property type="protein sequence ID" value="MESCA007821-PA"/>
        <property type="gene ID" value="MESCA007821"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402172_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577675" description="Peptidase A2 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007821-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10119" checksum="B9C8DAE9CBC47041" modified="2013-10-16" version="1">MLRSLAVTLQLGLLISHGGDLDLCFAPRLKKINSVFAAQVSGRFVSSNVEEHPLVSKIRGGDKVIHSNMRLWILDLGASHTAIAKRRNSQSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVR6</accession>
    <name>T1GVR6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02053102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVR6"/>
    <dbReference type="STRING" id="36166.T1GVR6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007882-RA">
        <property type="protein sequence ID" value="MESCA007882-PA"/>
        <property type="gene ID" value="MESCA007882"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036321_4_0_1"/>
    <dbReference type="OMA" id="MPIHETI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF7">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007882-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27338" checksum="4B94DA27B0302B26" modified="2013-10-16" version="1">MSDLQPFEKLTLYNYNSWVVIVKGHLMSKELWRYVDGTIEMPIVLPESKDFWSKKALQDAWKIKDNSALEFLLTSVTNGQRQHLKGCSTSAEAWENLRKVHIVSSPAEISTVIKRLRTTNYNKKNGMEAFIQKYYDIQTSLKGFKMPIHETILSIMMLQRLPKSFDAFVTAIEAQDSIPDFSSLKIKLLYEEQKIKSRENADDSKAFSGKGRGKCFNCGQRRNFADDCRNTKADLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVW5</accession>
    <name>T1GVW5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02118386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVW5"/>
    <dbReference type="STRING" id="36166.T1GVW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007933-RA">
        <property type="protein sequence ID" value="MESCA007933-PA"/>
        <property type="gene ID" value="MESCA007933"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_21_5_1"/>
    <dbReference type="OMA" id="FHATAVM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="37"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007933-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15159" checksum="D0424CC7205D7DDA" modified="2013-10-16" version="1">INCVSAIEYGSCHSFAGGSVFPEGDKSADHHLQYTKAIISKPAPSFEGTAVVNGEFRSISLKDFIGKYVVFFFYPLDFTFVCPTEILAFSDRVKEFHQINTEVIACSVDSHFTHLAWINTPRKEGGLGNIQIPLLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVW6</accession>
    <name>T1GVW6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02195252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVW6"/>
    <dbReference type="STRING" id="36166.T1GVW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007934-RA">
        <property type="protein sequence ID" value="MESCA007934-PA"/>
        <property type="gene ID" value="MESCA007934"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170584_0_0_1"/>
    <dbReference type="OMA" id="HKIMSAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010442">
        <property type="entry name" value="PET_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF20">
        <property type="entry name" value="PROTEIN ESPINAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06297">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PET" evidence="2">
        <location>
            <begin position="6"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007934-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12833" checksum="E45614C3555C8E96" modified="2013-10-16" version="1">IPSLLQVRLYFSQLPDDKVPYVNSIGERYRASNCCNNFHHKIMSASKFSVQLNPIHFPFKVRYCHSLTDEERKELRLFSSQRKREALGRGAVRLLSDERPCKGHGRKLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW92</accession>
    <name>T1GW92_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008064-RA">
        <property type="protein sequence ID" value="MESCA008064-PA"/>
        <property type="gene ID" value="MESCA008064"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888334_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7406" checksum="78588DF0EAAC9F47" modified="2013-10-16" version="1">MFADNLRSRAYRSASEVAASKSPWNTTTMWRPEATRDLKMVHNRDLFRDRVYKGPSRITFTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWB9</accession>
    <name>T1GWB9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-nitrophenylphosphatase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWB9"/>
    <dbReference type="STRING" id="36166.T1GWB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008092-RA">
        <property type="protein sequence ID" value="MESCA008092-PA"/>
        <property type="gene ID" value="MESCA008092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_3_1_1"/>
    <dbReference type="OMA" id="ECVANEF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF4">
        <property type="entry name" value="RE04130P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15849" checksum="53B861F9ED446B84" modified="2013-10-16" version="1">MRAHQYLMKNSNCLFLTGASDKKLSVKSGVNIMAPGYFMEMLAMAAKKVPIALGKPSKDLSDLVMEKFAIKDRKRVLMIGDMLEQDIGFGKNSGFQTMLVLTGGTKKDELLDDNNSHLIPDYIADGMIDFVKFFDDLNKSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWG6</accession>
    <name>T1GWG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWG6"/>
    <dbReference type="STRING" id="36166.T1GWG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008143-RA">
        <property type="protein sequence ID" value="MESCA008143-PA"/>
        <property type="gene ID" value="MESCA008143"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145018_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF3">
        <property type="entry name" value="KUZBANIAN-LIKE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27911" checksum="90166E2DB2514279" modified="2013-10-16" version="1">QQDQPQKSSKPANIIVKHDFETNLRKKFGNFPPISEDDSSKFNNNFFNSNWTAYNSSSSTSSSSSRPSHKTHVEIITKNGPTKKSNIIGSNYNPEVVSTSQNNNYHSHHHNNINFNIRNDISDSSFARALYDRKSTCMLYLQADHTFFQKMGSDEASIEAITRHVQRANSIYRNTVIQDMSTVCNLRNFQVLEHALGLITIDHKNKRTRHVFEENDLDIKLHSSYDIDVVSISQYLYSYILDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWH2</accession>
    <name>T1GWH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWH2"/>
    <dbReference type="STRING" id="36166.T1GWH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008149-RA">
        <property type="protein sequence ID" value="MESCA008149-PA"/>
        <property type="gene ID" value="MESCA008149"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016347_5_1_1"/>
    <dbReference type="OMA" id="PWHEHES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF5">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008149-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10396" checksum="BD4521654E2D7860" modified="2013-10-16" version="1">MEQHRLESFTTNWPFNNSDSCNVQNMAEAGFYFCGNDRENDTAACFVCGKVLDGWESTDVPIDEHRKHAPQCLFVKLGKTEKDFLRNLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWJ0</accession>
    <name>T1GWJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol-3-phosphate synthase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWJ0"/>
    <dbReference type="STRING" id="36166.T1GWJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008167-RA">
        <property type="protein sequence ID" value="MESCA008167-PA"/>
        <property type="gene ID" value="MESCA008167"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226219_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008167-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11845" checksum="60EC4274EB56E26C" modified="2013-10-16" version="1">MFTTLVIEPETLTLNLRTDRKVPKLGVMLVGWGGNNGSTLTAALEANRRKLQWRKRTGVQTANWFGSITQASTVLIGHDDNGKDVYLPMNELVPMVNPDDIDFIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWP6</accession>
    <name>T1GWP6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008224-RA">
        <property type="protein sequence ID" value="MESCA008224-PA"/>
        <property type="gene ID" value="MESCA008224"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624811_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008224-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9274" checksum="7E08AD646F4BD3D3" modified="2013-10-16" version="1">MKYHYKSSLLYKSQTIETKSLESSELPEFQIPQNQRRKNQKQFPLMSSEMSLRSQGKDEIVNFLYVIYGMNLHLNKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWV7</accession>
    <name>T1GWV7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWV7"/>
    <dbReference type="STRING" id="36166.T1GWV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008289-RA">
        <property type="protein sequence ID" value="MESCA008289-PA"/>
        <property type="gene ID" value="MESCA008289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729575_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8634" checksum="C9B2928EAAABBDC0" modified="2013-10-16" version="1">MFFSSRLTSTGDYYDDDDHECHSEHETSWDELEEHHRLENFTAGRERLQEFKGRIPPRKKKKEAPKTKPKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWX9</accession>
    <name>T1GWX9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008314-RA">
        <property type="protein sequence ID" value="MESCA008314-PA"/>
        <property type="gene ID" value="MESCA008314"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818007_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008314-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16611" checksum="CD23CF713A95E7C0" modified="2013-10-16" version="1">MVNGAIAKHNLHDGIIDNGMRVKELVSIDKNEELARKIGSVRKTSKETRELRKQAFQNKRREVVKLARQKKRRLKKAQFYENINRQRKAFARLTSCCKNKNGREKKLYLEYNKIPFFDVNTKIMGCFILKESTVVDVANQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX83</accession>
    <name>T1GX83_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02373795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008425-RA">
        <property type="protein sequence ID" value="MESCA008425-PA"/>
        <property type="gene ID" value="MESCA008425"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266792_0_0_1"/>
    <dbReference type="OMA" id="HECLEEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008425-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11861" checksum="2AEBF808E4C96AC1" modified="2013-10-16" version="1">MDISTKENFAACAKELDIQEDAYEKSPNHKCLFKCMMEKDGILKNGVFLEHEFKNVLTKDTELDENNRQKSIKALPICMDEAKYLTDLCNKAYTITVCLYKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX84</accession>
    <name>T1GX84_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX84"/>
    <dbReference type="STRING" id="36166.T1GX84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008426-RA">
        <property type="protein sequence ID" value="MESCA008426-PA"/>
        <property type="gene ID" value="MESCA008426"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801298_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052223">
        <property type="entry name" value="Actin_Cytoskeleton_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271:SF1">
        <property type="entry name" value="PROTEIN OUTSPREAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008426-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15753" checksum="2FE14C3060D425FC" modified="2013-10-16" version="1">MERPETIPQAIVDMTTVLEVSTAEDVTGHTNSIAITAPERVTFVKGTCPEESKWWLNILVAFPKSKGRHKRNATFPGGMSANNSSTNLRNRHNSYHKESMISPTIESSWNNVDVDDGSGSNDGDVPANGQDENNINAGNEINNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXQ1</accession>
    <name>T1GXQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA methyltransferase FtsJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008605-RA">
        <property type="protein sequence ID" value="MESCA008605-PA"/>
        <property type="gene ID" value="MESCA008605"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019427_0_0_1"/>
    <dbReference type="OMA" id="EFVTVAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004483">
        <property type="term" value="F:methyltransferase cap1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050851">
        <property type="entry name" value="mRNA_Cap_2O-Ribose_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121:SF2">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="2">
        <location>
            <begin position="124"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008605-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="685" mass="80793" checksum="D0931EACFD37C25F" modified="2013-10-16" version="1">MNFVPKRFPPRQNFAGPSSRTDGRISNESRANGRVSDEELNSYFGNSQSYNKNPNWTLPSISKIYTSPIYEEQKFQEIKEKMNNEKDKILENYSVEHWKEIKDKHALNPKIVKVLKMTLKCGNVTRNWCKLYEILKTFPLVKSGKINSVHIGDQHGGMITALNHYIHCNFEEVEWNWKAMCINPYYEGENYDNRSDDRLICHTYDNWTFGMDFSGNVIDPENISFFAKKCHEMGEIDLAICTQTTDTKLDPNNRENLKFDSHFAGMIAGLNALSNGGNLIMLSSTLFNFYMYFLNMVFHKVILYKPSCTPVSSSEIFVIGLSFKKDRNIEEYIEIMQTKVGPKSYEKGSLFGKEEIPSDFISQHYAFMEYFSKKYLENTKKIKFYEENPEDGHTKRWEIICRIMPKFFISKYDIKDISEEKKLIQFDNGLNIKFCSNMSKLTIDYKFSKDENCNFTTEILKCFDENDFTDQLMLIFEKFKATQELVYWPYCTEDKTLTNLKPIDLILEYGKPIERVRKTLFITPSIMYMFVNKIEDNFNIFIVESFKDEIECIFDEKIKIKYKFKTNYGDSERDFLKVVLNEWIAKKPKKIEFKNLLFITHFSASFLRILAVYYKYLTVNLKGYVEMEGFKRNKISGGSWDYVMCFTEYKQVNKGEYFEILSNYNSQLLMQYIVKTFDDLISKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXR8</accession>
    <name>T1GXR8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-guanine(15) transglycosylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXR8"/>
    <dbReference type="STRING" id="36166.T1GXR8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008623-RA">
        <property type="protein sequence ID" value="MESCA008623-PA"/>
        <property type="gene ID" value="MESCA008623"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1082679_0_0_1"/>
    <dbReference type="OMA" id="PSHHERC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050852">
        <property type="entry name" value="Queuine_tRNA-ribosyltrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="1">
        <location>
            <begin position="38"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008623-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="261" mass="29308" checksum="C659598014962390" modified="2013-10-16" version="1">MRESLEAFQKGITPFVGFPSAPSFLVMKDPCDVTPSGFNESGVSPLFGRKGKELLSPEKYLKLVEFFKPDIYHSLPDSDTNAESAKKRLRKSLNRNVDFFNKCLELHKKSEVLNKSMFFAPIIGGFCKITRTECVKEMKPKFQYVDGFVLDGFHTNGVPATKLNLSSLKEVVSVTLQELGEKQKPIAMLGAYSPLAVLEMVKLGVDVFDSSYVYISTKHFKSLTFNFDLSKKVDANQFSLDLSDPNVFPILMNDMHRGALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXW9</accession>
    <name>T1GXW9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008681-RA">
        <property type="protein sequence ID" value="MESCA008681-PA"/>
        <property type="gene ID" value="MESCA008681"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940500_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008681-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14739" checksum="46198C2426EB4FFF" modified="2013-10-16" version="1">MQSQILAAVPFDRKFIYLDRTSQIRLKISSDICFQAVSSFLFPILLSDVTSKRGLLIFRKIIVLNGENWSNVKAAGTTTGNFLTIRGFRLGNAFPCRCFNIQRKLIMVSANNTRTVLEKYDVSITTLQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY91</accession>
    <name>T1GY91_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02378789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY91"/>
    <dbReference type="STRING" id="36166.T1GY91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008814-RA">
        <property type="protein sequence ID" value="MESCA008814-PA"/>
        <property type="gene ID" value="MESCA008814"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451116_0_0_1"/>
    <dbReference type="OMA" id="WRSETCK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF68">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="8">
        <location>
            <begin position="40"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008814-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="21447" checksum="579635E20343C3FA" modified="2013-10-16" version="1">MEYGMNVYASNKIAMDRAVKDTRLEECKHWNYPYDLPKTSVIIVFHNEGLSVLMRTVHSVINRSPKHVLQEVLLVDDFSEKEDLKGKLDEEVKSFNGLVRLIRNEERGGLIRTRSRGAQEARGEVIVFLDAHCEVNKNWLPPLLAPIYRDRTTMTVPKSMELTTKPLNTVQFIVAVIITEEFSNGEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU4</accession>
    <name>T1GYU4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GNAS complex locus</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYU4"/>
    <dbReference type="STRING" id="36166.T1GYU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009031-RA">
        <property type="protein sequence ID" value="MESCA009031-PA"/>
        <property type="gene ID" value="MESCA009031"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157663_0_0_1"/>
    <dbReference type="OMA" id="REDKHNN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007191">
        <property type="term" value="P:adenylate cyclase-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034330">
        <property type="term" value="P:cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000720">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF212">
        <property type="entry name" value="G PROTEIN ALPHA S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009031-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10739" checksum="45F7CBD9844D0EDC" modified="2013-10-16" version="1">MGCFGSAESKQSDDDDRLQKRRSEQITKQLQKDKQIYRATHRLLLLGAGESGKSTIVKQMRILHVNGFSNEERKQKIDDIKKNIRDAIIKKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYX1</accession>
    <name>T1GYX1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009058-RA">
        <property type="protein sequence ID" value="MESCA009058-PA"/>
        <property type="gene ID" value="MESCA009058"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870142_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009058-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7859" checksum="18EB1B13009C8D85" modified="2013-10-16" version="1">MFISFREKNPLDNLRSDVRKRKRRPKKVRAKRLRWAGHLLRQPQNRPAQTLHKSNFQFSQDLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYZ7</accession>
    <name>T1GYZ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYZ7"/>
    <dbReference type="STRING" id="36166.T1GYZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009085-RA">
        <property type="protein sequence ID" value="MESCA009085-PA"/>
        <property type="gene ID" value="MESCA009085"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052293_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFD" evidence="1">
        <location>
            <begin position="41"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009085-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13372" checksum="FCB566F9D0BEC23C" modified="2013-10-16" version="1">MVTNEFYSSSSSEEFYENPGTYSLYITHAIDGILSKTPKSALCATWGGVNIKTFDGIVYKSPLSCSHTLVSDKVDGTFDVILKACPYGSDFDCPHVIKIFWQSIIYTIENIKVENVNEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZP2</accession>
    <name>T1GZP2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009346-RA">
        <property type="protein sequence ID" value="MESCA009346-PA"/>
        <property type="gene ID" value="MESCA009346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199281_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6776" checksum="42D655471DDD6239" modified="2013-10-16" version="1">MDDDLLAKQLFSKAPNGTRRSRERTRWGYQDGEEQLALETRGGGKSIQRRPPILITVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZT1</accession>
    <name>T1GZT1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02120279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZT1"/>
    <dbReference type="STRING" id="36166.T1GZT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009387-RA">
        <property type="protein sequence ID" value="MESCA009387-PA"/>
        <property type="gene ID" value="MESCA009387"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207419_0_0_1"/>
    <dbReference type="OMA" id="MGDANEV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009387-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="26279" checksum="6179D71842D3C719" modified="2013-10-16" version="1">MGDANEVMNMLLQNQIEGGLPDDDPQTTYLITAWARICKILGKQFEQYLPLVMGPVMRTAQMKPEVALLDNDEVQDIEDDVDWSFINLGEQQNFAIRTAGMEDKASACEMLVCYARELKEGFAEYSEESIESLGPNCLSEESMKQILEIITKYMVEHFERADKRSLARHEEDYDDGVEEALAEEDDTDIYLLSKVADIIHALFLTNKVNFLPFFDQVSMHFIKLLDASRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZZ8</accession>
    <name>T1GZZ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TFIIS</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFS-II family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02149857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZZ8"/>
    <dbReference type="STRING" id="36166.T1GZZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009460-RA">
        <property type="protein sequence ID" value="MESCA009460-PA"/>
        <property type="gene ID" value="MESCA009460"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037637_4_2_1"/>
    <dbReference type="OMA" id="MFECELG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TFIIS_cen_dom"/>
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    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
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        <property type="entry name" value="IP08861P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS central" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="10">
        <location>
            <begin position="37"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009460-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9169" checksum="F145D3DC09F875EE" modified="2013-10-16" version="1">MTPEEMASDEMKRIREKFVKEAINDAQLATVQGTQTDLLKCGKCKKRNCTYNQLQTRSADEPMTTFVMCNECGHRWKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H163</accession>
    <name>T1H163_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Histone H4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H4 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="MESCA009917-PA"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
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    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020836"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009917-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9728" checksum="569290B713A68C5D" modified="2013-10-16" version="1">MLPFSAKPTQNVCFIGVDTEGCKHRLRPPFSTSTYPRNDQKVFLESFIRDAVTYTQNPKRKTVTVMDVFYTLKRQGRTWYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1G7</accession>
    <name>T1H1G7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1G7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010028-RA">
        <property type="protein sequence ID" value="MESCA010028-PA"/>
        <property type="gene ID" value="MESCA010028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294859_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11409" checksum="4064D15825574174" modified="2013-10-16" version="1">MHYQQQSKQQQMQANGMIMTGNHNIRAPRPPVRTVSSSPSPSEQMQQQQQQCNVYNNSGNLQSTAGKSTNIPQQQQQIILVDAYKEVCPLLKKRKILGKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1X2</accession>
    <name>T1H1X2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1X2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010196-RA">
        <property type="protein sequence ID" value="MESCA010196-PA"/>
        <property type="gene ID" value="MESCA010196"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134025_0_1_1"/>
    <dbReference type="OMA" id="QFICQTI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685:SF2">
        <property type="entry name" value="825-OAK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685">
        <property type="entry name" value="825-OAK-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010196-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="15983" checksum="CD64C6894E933A4C" modified="2013-10-16" version="1">MYIHYQFQFICQTITNKMFKITLAIFAVIACAAAKPGLVAPLAYTAPVVDAPGVVTATSSQFIARNHHGIVAPVVAPVAAKIVAPAPLAYTAPIVKAAAPLAYTAPVAYSSPIVASAPIVKAATPLAYTAPVAYSSPLVHSHLASPLTYSAPLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Y0</accession>
    <name>T1H1Y0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02375636">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Y0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010204-RA">
        <property type="protein sequence ID" value="MESCA010204-PA"/>
        <property type="gene ID" value="MESCA010204"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3130234_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010204-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="50" mass="5752" checksum="278FC43FC3B5B8FE" modified="2013-10-16" version="1">MDLYTYTSSYHVMLEQSASPSGEQQQKTVKYGRQLLIKRNLIEGTPSLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H319</accession>
    <name>T1H319_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-[acyl-carrier-protein] reductase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-enoyl thioester reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H319"/>
    <dbReference type="STRING" id="36166.T1H319"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010630-RA">
        <property type="protein sequence ID" value="MESCA010630-PA"/>
        <property type="gene ID" value="MESCA010630"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412103_0_0_1"/>
    <dbReference type="OMA" id="PAICGNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08290">
        <property type="entry name" value="ETR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="9">
        <location>
            <begin position="23"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010630-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="21048" checksum="4A061348FC00BE64" modified="2013-10-16" version="1">MRVQALGCKDIVCYVEHTDSLVDFAKIFLGKYPSKPALPAICGNEGLGEVVAIGDKVEGFEIGDRIVPNANHLGTWATHIKRPEEDFMKVRSSIDPAALCQINVNPPTAYRMLKDFVSLQPGDTVIQNGSNSAVGQAVLQLSRAWGLKTIAVVRDRPDIINLVSNLKCLGATEVLTEDQANATDKFKNGEWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3L7</accession>
    <name>T1H3L7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3L7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010846-RA">
        <property type="protein sequence ID" value="MESCA010846-PA"/>
        <property type="gene ID" value="MESCA010846"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838551_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010846-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7887" checksum="A17B9E7EC5C50CCE" modified="2013-10-16" version="1">MIAFYIILYIKMIAMSKSLEEEYDEFCAEIITFQKKFGPIKQLCLRPYYFKIKGINSHQTSKKQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3N2</accession>
    <name>T1H3N2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3N2"/>
    <dbReference type="STRING" id="36166.T1H3N2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010863-RA">
        <property type="protein sequence ID" value="MESCA010863-PA"/>
        <property type="gene ID" value="MESCA010863"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827510_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010863-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16401" checksum="9EFAAB914E13AFD7" modified="2013-10-16" version="1">MTKYPIVLIVSEGQTTPSEIISNIRKLPSEEDVESLGFFPTISDSINGHIFHLKTKYYTTDLYLVPFQEDISRFPLELQEDIEGVLIYFDSGVRSFSKRIPIYANYLNLTILKWEFCSVIICMKILMRGSHTRRRSRKVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4A7</accession>
    <name>T1H4A7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4A7"/>
    <dbReference type="STRING" id="36166.T1H4A7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011115-RA">
        <property type="protein sequence ID" value="MESCA011115-PA"/>
        <property type="gene ID" value="MESCA011115"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504890_0_0_1"/>
    <dbReference type="OMA" id="HHPTIIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011115-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="540" mass="57166" checksum="E6C8027D00148CE1" modified="2013-10-16" version="1">IECDRIQIRARLLNRNSRLVNLKKSHKKRGVFHNHAGLILQPQHKIYGLTPLAAPIGHAHVTHTAHGAKFSLPHAHVQKFHKHIPLPSAVLPAVNVPHFHQHVDVPVAAPIAPHPVVETPSVGEAALAQPIQPPVSNVHPIPFTPEVHHHAAIIPQGIHHHAAIIPQGIHHAAIIPQEHHHASSVYATSGGVIGGQPFILRPGNAVVQSYNVNYARPVAHAHIPVAHAHVPVAHAPVAHTPVAVAETPIQFAHTPVSAVASAPIFPSPSVVSISGHHNYFVPRPVAAAPVAPVAPAFPVFPSPSAAPAFPVFPSPSAAPALPVAPSQPVFPSPTPAVPVFPSPAPAPSFPATPVFPAPRPFIPSPVAPPQRPIFSLPVPQNPFFIGQGLPEATPTVLSERPIVVPDSQQPEIPSSFPELPASFPSGPGASFPSGGSDILPGQIPSQGSDILPGQIPAQPPFQNVPHNHGHQVFSILSPQLYLISPQMPNNLLLNYHQFMVTLNQALVVHLATIIQTQTSTRNGNLFEELVADKKLESNCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4P2</accession>
    <name>T1H4P2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02384743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4P2"/>
    <dbReference type="STRING" id="36166.T1H4P2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011258-RA">
        <property type="protein sequence ID" value="MESCA011258-PA"/>
        <property type="gene ID" value="MESCA011258"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161220_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.38.10">
        <property type="entry name" value="Luciferase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011258-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12395" checksum="49EADB75201A8F74" modified="2013-10-16" version="1">MKSLNIFFSSGAPLPCETLKNFKEILSPNCRIIQGFACTEKGGITMDLYGKVYGSCGTVAPNVEVKIIDDNGHSLGPNENGEIVARNFHPWKGYYNDEEITREKLNFSEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4S0</accession>
    <name>T1H4S0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02383754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4S0"/>
    <dbReference type="STRING" id="36166.T1H4S0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011288-RA">
        <property type="protein sequence ID" value="MESCA011288-PA"/>
        <property type="gene ID" value="MESCA011288"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124087_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="2-oxoglutarate dehydrogenase E1 component N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011288-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13275" checksum="2914DBE293AD17A2" modified="2013-10-16" version="1">MMTSILFTGQRGRNYLQVLSAARSYSSSVNKSSTNYVEDMYSKWLRDPTSVHISWDAYFRNNYPNASAMAVGQPDEKIIDDHLAVQAIIRSYQSRGHLAAELDPLGITNPESSISKDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H583</accession>
    <name>T1H583_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02129313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H583"/>
    <dbReference type="STRING" id="36166.T1H583"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011458-RA">
        <property type="protein sequence ID" value="MESCA011458-PA"/>
        <property type="gene ID" value="MESCA011458"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313161_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011458-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="10885" checksum="641D654491985A03" modified="2013-10-16" version="1">CSLAVQLAKVRESVLIISTDPAHNISDAFDQKFTKVPTKVNGFENLFAMEIDPNAGLGELPDEYFEGESDAFKLSKGVFNEIVAALPGIDEAMSYAEVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H586</accession>
    <name>T1H586_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H586"/>
    <dbReference type="STRING" id="36166.T1H586"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011461-RA">
        <property type="protein sequence ID" value="MESCA011461-PA"/>
        <property type="gene ID" value="MESCA011461"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212951_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011461-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12141" checksum="DFB3EC706E5D5D0F" modified="2013-10-16" version="1">MNLGGVLWNPKIEECISRALMSTRTGSILGKLLSYITATCFEATDARTPDHPKAPLGNNYRPTQPLLHRPVRTNIDFKPSKQNGGKVCATMYRDFHYKEKEEDFDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5M4</accession>
    <name>T1H5M4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="1">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="1"/>
    <dbReference type="STRING" id="36166.T1H5M4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012007-RA">
        <property type="protein sequence ID" value="MESCA012007-PA"/>
        <property type="gene ID" value="MESCA012007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_580859_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF116">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE CG5065-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="455"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="2">
        <location>
            <begin position="119"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="471" mass="53657" checksum="80059C418712F0E9" modified="2013-10-16" version="1">GTGFLGTVLIESLLDSHPDIGNIYVLVRGKKRFDPNERINKLLQKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAKKLKNLSSYIFVSTAFCNSNNRGLIMETVYKSKYDPHEMLKMAEDDLAWEGFDDIKCKEIIGNHPNTYTFTKNLAENLMMSDMSGFPAAIVRPSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFDYFLDKDFRYSLKNSLSVSSRCDKERYTWDASLCDWSEFIDRCLIGVRHFYFKESAETTQWHKQYWAAFNVLHYAGLFAMLLVFLFFRSVFGSTLGYGLAFALWCFLMWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5R5</accession>
    <name>T1H5R5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5R5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012048-RA">
        <property type="protein sequence ID" value="MESCA012048-PA"/>
        <property type="gene ID" value="MESCA012048"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_2_1"/>
    <dbReference type="OMA" id="WLMITAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012048-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="525" mass="60718" checksum="B188F68D146B0BF2" modified="2013-10-16" version="1">FTNRLAEEKSPYLLQHAHNPVNWWPWGDEAIQKAKSENKLIFLSVGYSTCHWCHVMEKESFENEEIAKVMNENFINIKVDREERPDIDKIYMNFLLMSKGSGGWPMSVWLTPNLAPIAAGTYYPXXXGKGGIHDHVFGGFARYSVDRQWHVPHFEKMLYDQGQLMSSYANAYKLTKDRAHLVHADKIFRYLMKDXHPLGGFYAGEDADSLPAAGDTVKVEGAFYAWEYNEVRDEIAENSDQFPDYDDVFQVFDIYCYHYNLKENGNVEPTSDPHGHLLNKNIPIVKRSLQDTCDKFQIEEEKLKILLDTVNEILHKVRDKRPRPHLDTKIITAWNGLILSGLSKLACCCDIGAEKRNEYMQAAKELIDFLKKYSFDKRQCSTEISLWRGKEKIHGFLDDYAFLIKGLLDYYKASLDFSGLEWAKELQDIQNQHFWDEKYGGFFYSQANNPNVIIRLKEDHDGAEPTGNSVAARNLVLLSHYFDLEEYNKSMTFNFFADTDHFAYSLPEMMSGLLLHEHGLDLVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H646</accession>
    <name>T1H646_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="T1H646"/>
    <dbReference type="STRING" id="36166.T1H646"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012179-RA">
        <property type="protein sequence ID" value="MESCA012179-PA"/>
        <property type="gene ID" value="MESCA012179"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_1_0_1"/>
    <dbReference type="OMA" id="PFYVWDL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061708">
        <property type="term" value="F:tRNA-5-taurinomethyluridine 2-sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.280:FF:000001">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="13">
        <location>
            <begin position="170"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="12">
        <location>
            <begin position="258"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="313" mass="36178" checksum="0B3DA1134DC71465" modified="2013-10-16" version="1">MKNWDKDETGQCLGEKEYSDAEYVCRKLEIPLFKVNFVKDYWNNVFSEFLDDYKSGLTPNPDILCNKHIKFNAFYEYAKKNIKDAISTGHYARTSFGPFLENYDRGMDSFKDQTFFLSGIRQDVLQRTMFPLGEYLKKDVKDIAIKIGIPKLAVKKESTGICFIGKRNFQNFIQEYIPSKPGNFIDIDTKKIVGEHNGIHTWTIGQRCRIHSLNPYYVAKKVPETGDIYVASDEPSIILEQHISGECFMDFRKLNCVNFRCFFRFQHTKPLVSCFLQSVPDSNQIVVTLDKPLRAITPGQYSVFYNGEECLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6L3</accession>
    <name>T1H6L3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6L3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012346-RA">
        <property type="protein sequence ID" value="MESCA012346-PA"/>
        <property type="gene ID" value="MESCA012346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_464318_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="588" mass="63401" checksum="02CE9BD1845F6D72" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGESIDSLCFIVTGSLEVIQDDEVVAILGKGDVFGDQFWKDSAVGQSAANVRALTYCDLHAIKRDKLLEVLDFYSAFANSFARNLVLTYNLRHRLIFRKVADVKREKELAERRKNEPQLPQNQDHLVRKIFSKFRRTPQLHTGSKEQVSAPASDVEKGDQLPAKLTLTEESRILTMASAPSPSPSPSPSSGPPSARSTRASKWGRLLGSSSVDSASDVSTKVAVSRSLSARESLRESAAQGRQNSSSSSNGGQGNKXXXXXXKLPASQATLARQDTIDEGGEVDTSPPSRERERNLALERERQIEMASSRATTSCTYDTGLREEPPTLAQRDLIATVLDLKVDVRLEMQRMSQRIGKIEDLLQELVKESLQDSSGQTTPGDGIDDTCDSIIANATTAASSSGEPTTTPSTSTFCGAVGSGSSQAGGSSDVVITPTSTTTNISSAPPTTSQACASGGNGLGPILLKKRRSKSRKAPAPPKHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6X2</accession>
    <name>T1H6X2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EnsemblMetazoa" id="MESCA012454-RA">
        <property type="protein sequence ID" value="MESCA012454-PA"/>
        <property type="gene ID" value="MESCA012454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2139706_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12450" checksum="CB0343E4A85A21F9" modified="2013-10-16" version="1">ICKSFTERSKVMLCVICSVSEFGNTPKTIPSRQGSPFGIPFKASVRRGGRKRPVPTLTLSSIHCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H728</accession>
    <name>T1H728_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein O-mannosyl-transferase 2</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="STRING" id="36166.T1H728"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012510-RA">
        <property type="protein sequence ID" value="MESCA012510-PA"/>
        <property type="gene ID" value="MESCA012510"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_5_3_1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="344"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012510-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="426" mass="47694" checksum="24ACEFAB27A91AA7" modified="2013-10-16" version="1">MLIGLSGKLTGYNGTYPFEKPGDKYNGTNYQGMRIFCTTLGSLITPIAFDSVYDMTGSLQAAFISAVGMITLNQYILLDPILLFFMMGSVWGMVKISKYTSQQRSYTGKWWFWLFFTGSMLACTISVKFVGLFVVLLVGFNTIQQLWTILGDLNNTVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNGIGDANDVWKIVVPGAKDNDTVRTVTTKFTLLHLLQNCVLTSSGKQLPKWGFDQAEVSCNLNVRDKNAYWNIEDNVNKKLSKVNFEVYAPGFYERFIESHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H734</accession>
    <name>T1H734_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H734"/>
    <dbReference type="STRING" id="36166.T1H734"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012516-RA">
        <property type="protein sequence ID" value="MESCA012516-PA"/>
        <property type="gene ID" value="MESCA012516"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_10_0_1"/>
    <dbReference type="OMA" id="CSEQLYG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012516-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21985" checksum="6321BFDE8A9F641C" modified="2013-10-16" version="1">RKKDCVTIATDSRFGVQAQTIDTDFEKIHQDAPKMFLGLVGLQIDIRFLTVRERLAFRKSYEIRESREMTPKVFSSMFSNFLYERRLGPYFIVIAGLDPETSEPFICNMDLIGYPNLPDDFVVEGLCGEQLYGMCATLWKLYLEPDPFFEVISQSIFNAFDRDAISSWGATVYIIEKDKITVKKLKTRMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H780</accession>
    <name>T1H780_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H780"/>
    <dbReference type="STRING" id="36166.T1H780"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012618-RA">
        <property type="protein sequence ID" value="MESCA012618-PA"/>
        <property type="gene ID" value="MESCA012618"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_5_0_1"/>
    <dbReference type="OMA" id="CEELTLY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF15">
        <property type="entry name" value="RIBOSOMAL PROTEIN S30, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012618-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14639" checksum="C3F16C9A54598964" modified="2013-10-16" version="1">MQIFVRGFDALEALEVNNNTSVAEIKRLVPTQEFNCEELTLYCQSAQLADDLFVECLPSAELDCEVGLLGGKVHGSLARAGKVKGQTPKVEKQEKKKKKTGRAKRRIQYNRRFVNIVQGFGRRRGPNANSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H795</accession>
    <name>T1H795_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
            <ecNumber evidence="8">4.1.1.130</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Coenzyme Q biosynthesis protein 4 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ4 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.130" evidence="8"/>
    <dbReference type="STRING" id="36166.T1H795"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012633-RA">
        <property type="protein sequence ID" value="MESCA012633-PA"/>
        <property type="gene ID" value="MESCA012633"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061241_1_1_1"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="26411" checksum="16AE76364A0CAEEB" modified="2013-10-16" version="1">ETLDEFTREFLETRIELTPIQKTILIAGSAVASLLNPRRHDMIACLGETTGDLSLSKNLELMKSTEEGQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNVTPDSRMKVMFMDDPELAYIMTRYRECHDLIHTILGMPTNMLGEVAVKWVEALNIGLPMCYGGAVFGALRLRPKQRIEYKRKYLPWAFEVGNKSKPLMNVYWEKRWEQDIEDLRKELNISVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Y4</accession>
    <name>T1G9Y4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02055229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Y4"/>
    <dbReference type="STRING" id="36166.T1G9Y4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000026-RA">
        <property type="protein sequence ID" value="MESCA000026-PA"/>
        <property type="gene ID" value="MESCA000026"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136302_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053027">
        <property type="entry name" value="AGGF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23106">
        <property type="entry name" value="ANGIOGENIC FACTOR WITH G PATCH AND FHA DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23106:SF24">
        <property type="entry name" value="ANGIOGENIC FACTOR WITH G PATCH AND FHA DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="40"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000026-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12633" checksum="C1E51D6760110F33" modified="2013-10-16" version="1">MAISKITDIANPSYLRFVVQETNLEIIQIGTLFIPTYKGGSLGKEGNHDVILPDIKVSQYHLKFQYDEKSSMYKCIDLGSRNGTILNGKRMSSSSRIRACCTTRINKAAFSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAK1</accession>
    <name>T1GAK1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAK1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000259-RA">
        <property type="protein sequence ID" value="MESCA000259-PA"/>
        <property type="gene ID" value="MESCA000259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199963_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12449" checksum="92D189EA4E09219F" modified="2013-10-16" version="1">MADQKFEDFSCTCRNSHAFSTLHYFAAAHFIPLYSMHIALQCNSEPKCIIFLFHPIPFIPNSQSNLKKGTTTKSKSSLSCFFYLIFREGLSFTGTWFCKDRKCEDLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB07</accession>
    <name>T1GB07_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02055519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB07"/>
    <dbReference type="STRING" id="36166.T1GB07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000427-RA">
        <property type="protein sequence ID" value="MESCA000427-PA"/>
        <property type="gene ID" value="MESCA000427"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_995958_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052612">
        <property type="entry name" value="ANP_Clearance_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001170">
        <property type="entry name" value="ANPR/GUC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755:SF11">
        <property type="entry name" value="ATRIAL NATRIURETIC PEPTIDE RECEPTOR 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755">
        <property type="entry name" value="NATRIURETIC PEPTIDE RECEPTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00255">
        <property type="entry name" value="NATPEPTIDER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Receptor ligand binding region" evidence="8">
        <location>
            <begin position="43"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000427-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="280" mass="31288" checksum="26AD5F9FAE11B528" modified="2013-10-16" version="1">MYLRSSNDSNEHNKISKELYFVVLATGSVENNNNDCILVKLLSTIELAIKKVQSDRLFGVNIDVTIISRDTFCSSTYGSIGFFEIYSNFKEKINAILGLPCDYVLAPISRHASVWEIPVLSTSGRSRAFDDKSFTYSTLTRLMGGVGAMGESMKGLINSFNWTKSAFIVQNSDEDVGNSVCFLCIISIDEKIGNKGLKSYKFSKELSEKNAIEALTKISESARIVIMCADKKSIRRIMLSAQDMNMIDSGEYVFINIELFSNKYQLKSMLLNSKTILVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBW0</accession>
    <name>T1GBW0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000749-RA">
        <property type="protein sequence ID" value="MESCA000749-PA"/>
        <property type="gene ID" value="MESCA000749"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326659_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000749-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="10819" checksum="DE8209EE1B6F10ED" modified="2013-10-16" version="1">MEAGLPICRVCYQPATFFQVSGGIQIPTCTKCSGSVYPGQNLFLESTAEGVLPGQFIIQPTELIPTYQIVQDNTNHNVVSNRNNSTKNSVDMDKNLPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBX6</accession>
    <name>T1GBX6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02128653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBX6"/>
    <dbReference type="STRING" id="36166.T1GBX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000766-RA">
        <property type="protein sequence ID" value="MESCA000766-PA"/>
        <property type="gene ID" value="MESCA000766"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011511_0_2_1"/>
    <dbReference type="OMA" id="RTHIMPI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF10">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="13">
        <location>
            <begin position="19"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000766-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="236" mass="26780" checksum="EDA4E0277D88BC55" modified="2013-10-16" version="1">MPGNGNGNNTNNSQGKTLGMTSAISLAPPKPEDIKKTEELHHSLEPYNVFETDEELNHRMEILAKLNVLVKEWVRDVSISKNMPLETADKLGGKIYTFGSYRLGVHHKGADIDALCVAPRNIERADYFASFFEMLKKQTEVTECRSVEEAYVPVIKMNFDGIEIDLLFARLSLKEIPDDFDLRDDNLLKNLDPRSVRSLNGCRVTDEILALVPNIENFRLALRTIKLWAKKFSLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC97</accession>
    <name>T1GC97_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000905-RA">
        <property type="protein sequence ID" value="MESCA000905-PA"/>
        <property type="gene ID" value="MESCA000905"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2489744_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000905-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9972" checksum="0EEB7A16A54F9E68" modified="2013-10-16" version="1">MVSVADQSVLLQYWHVFLPLFLLFAAFLYLQNKRELEAYTKASQKVREHNKLDGADLVAVSEEDIDQEDEFSPSLETKEHVPFKGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCW2</accession>
    <name>T1GCW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCW2"/>
    <dbReference type="STRING" id="36166.T1GCW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001135-RA">
        <property type="protein sequence ID" value="MESCA001135-PA"/>
        <property type="gene ID" value="MESCA001135"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_555027_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="170"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001135-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="492" mass="55317" checksum="301516CB5F9A4B91" modified="2013-10-16" version="1">LRKIRSLLINNVQNVIVYLKDLSISFLDILKELRSQVIREACITIAYMSKCIRNKLDQFCIYILQELINLIQNSAKVISSAGTVALKYVIKYTHAPKLLPIITTTYTQSKSKDIRSTLCDILCLIFEEWQTKSLERNATTLRDMVKKGIADADNDARRNSRRAFWAFRKHFPDIADQVYSGLDPATQKTLERERDILSGNGGSMSASLRGSNSSLNSMPGGVIKRRTSTGLRSPATHTPSAQKSLEAFTELIKRLGPDFNAYTATVFPHVIDRLGDSKDTVREKAQLLLHNLMEYRVLTPQQLIDKLTPCFKHKNSKVREEFLQTIVNTLNEHGTAQLSVKSYIQPIVSLLGDPTSTVRDASIHTLVEIYKHVEQKFDEVKEEGLLLKSATSSHANGNGFQDETDTTSLQPPVRPTRLVKRTVSASAKKPTSLDVQPSLGDAGAISWEVFEASFEQVPSINIYTQKDIEDHFKNIIQIIGNKNVDWEKRVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDI0</accession>
    <name>T1GDI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02053645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053646">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053648">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDI0"/>
    <dbReference type="SMR" id="T1GDI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001367-RA">
        <property type="protein sequence ID" value="MESCA001367-PA"/>
        <property type="gene ID" value="MESCA001367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248068_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF44">
        <property type="entry name" value="ODORANT RECEPTOR 13A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="25465" checksum="9129E838689A0134" modified="2013-10-16" version="1">MRFDKAQFEMLEFMGDRNQARNHGFSMRFPYYSRFPFIENIDNLWVYTLAYIQQAHVGYVAIVCSISADMLLFSLIGILLMNFRYVQTRMETFQVQGTNEDLLRLKTIIHSHQMVLSMADLTNEIFSTTILLNFLSSIIIICMAAFQTTADNVPLIQLFSFFAMLLLELVQTGAISFLGQKLIDYTMPIPGEVPPSSNGVDLYNEKAPRKTESFLYRISTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDI5</accession>
    <name>T1GDI5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="MESCA001373-PA"/>
        <property type="gene ID" value="MESCA001373"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001373-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9302" checksum="C6CC5E48954FEE0F" modified="2013-10-16" version="1">MKAIKFIENFHKAVKYLIAEVKKGPQNGLKAAQKLAKKAQSSCKNVDKILTKQIIKKMSTVNVNNLVKAFKNVCKLIVSDEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDL1</accession>
    <name>T1GDL1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000920">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA001400"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631246_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001400-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="17843" checksum="F19F2D08CC346940" modified="2013-10-16" version="1">MTVKPTTTTVKPTTTTVKPTTTTVKSTTTKKPSFNPNCDCEVSSTLVPYKGTCNQYCDCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTGHWFPENVDCPYDNSTTITTNPTTTTTTKPTTTPVPSFNPKCDCGQSNSYVPYEGSCTKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDP0</accession>
    <name>T1GDP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDP0"/>
    <dbReference type="STRING" id="36166.T1GDP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001431-RA">
        <property type="protein sequence ID" value="MESCA001431-PA"/>
        <property type="gene ID" value="MESCA001431"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_738270_0_0_1"/>
    <dbReference type="OMA" id="SIKYMAG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01272">
        <property type="entry name" value="PTB1_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039576">
        <property type="entry name" value="APBB1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058:SF8">
        <property type="entry name" value="PROTEIN FE65 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="4">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="3">
        <location>
            <begin position="278"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001431-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="42750" checksum="F24B4F8BA5E06431" modified="2013-10-16" version="1">MDPQRIERNSHLYEEILSELQHQNSLKSGKPHMVSNRKNYAKINIESMGVDLKENFNMKQKKQSQEHPQDTNKVDSVVDGGGGVGTNDNNDSNVQNQNSIKYMAGNLNSEDLYALPNKRSNNTNNHKKNDETNEKVKEARASDEEDDDKDNDKSDEEKGKKEVIEGIEDKDATKDLPPGWEKHEDNDGPYYWHIKSGTIQREPPIWPKQPVKELKTPVNGPIIPFVIPTRQSTHQPFFGNRNSMILPETFSVTRSNTSFALDQDEERKRREDLTLKPIRFAVRSLGWVEIAEEDLTPERSSKAVNKCIVDLSLGRNDLLDVVGRWGDGKDLFMDLDEGALKLIDPENLTVLNTQPIHTIRVWGVGRDNGRWFNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEH9</accession>
    <name>T1GEH9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEH9"/>
    <dbReference type="STRING" id="36166.T1GEH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001743-RA">
        <property type="protein sequence ID" value="MESCA001743-PA"/>
        <property type="gene ID" value="MESCA001743"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_1"/>
    <dbReference type="OMA" id="TNICAMS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="43"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001743-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="271" mass="29509" checksum="C98B2706817006A3" modified="2013-10-16" version="1">MVCGSQRGEFKVSRDDHCAIRFGFRDAIPSVIARSIWESLLWGTIISTKFVATAAHCVDSIEDPSDILIRLDSNDRTKGGILDLAKAIKYHEEYDEETLENDIALIQMRVPIKGVHTADLAYTDFPEGTEVLVSGWGMESEDASELPTKLKAGTFKIVSLEECRRIYGEDVIFDTNICAMSDDSNVCPGDSGGPLTIEGIFLGVISWGYGCNRAGFPVDTCQGDSGGPLVVDDILVGVVSWGIGCAKPGYPGVYTSVPKFLQWIEDTILEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF10</accession>
    <name>T1GF10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001938-RA">
        <property type="protein sequence ID" value="MESCA001938-PA"/>
        <property type="gene ID" value="MESCA001938"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932813_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001938-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14265" checksum="3FE224754E58BC30" modified="2013-10-16" version="1">INFEKLCLVNGLGHEQEQTPQQPIYYHSEGYDPIEINVEKKKRNNEMNPRPHMTNNGPMGDRSRTAGTRSLSNSGGNGGMMRQGNNGGNNMMRGGSRSQGFNRGGMDNRGPPPNRGNSTTGGGGNNNYGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFB6</accession>
    <name>T1GFB6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFB6"/>
    <dbReference type="STRING" id="36166.T1GFB6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002053-RA">
        <property type="protein sequence ID" value="MESCA002053-PA"/>
        <property type="gene ID" value="MESCA002053"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1016671_0_0_1"/>
    <dbReference type="OMA" id="THEMPRS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2280.10:FF:000002">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clu" evidence="2">
        <location>
            <begin position="191"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002053-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="274" mass="30864" checksum="600CBAF860072587" modified="2013-10-16" version="1">MDPADAYNGVDCTSLSFLHTISQGDISEKKKTRPDSVDCTPPEYIMPAEANETPLLPLQPGIKNNKLPQAIKILTTSAWNPPPGPRKLHGDLMYLYVVTQEDKRFHISACPKGFYINQSTDDIFNPKPDTPSHLCHSLIDLLSQISPTFKRTFAQIQKKRTLRHAFERVATPYQVYSWTAPVLDHTIDAIRAEDAFSSKLGYEEHIPGQTRDWNEELQATRELPRKTLSDRLLRERAIFKVHGDFVIAATRGAMAVIDGNVLAINPGEDPKMQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFI0</accession>
    <name>T1GFI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in Wnt signaling. Plays a complex role in regulating the basal catalytic activity of the alpha subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02025559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFI0"/>
    <dbReference type="STRING" id="36166.T1GFI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002122-RA">
        <property type="protein sequence ID" value="MESCA002122-PA"/>
        <property type="gene ID" value="MESCA002122"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309208_0_0_1"/>
    <dbReference type="OMA" id="XCSHCED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000002">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002122-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11479" checksum="18F99745807E3CBB" modified="2013-10-16" version="1">MILILGLSDIPGEAMVKTYCPKCIDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPTNQFVPRLYGFKIHSLAYQIQLQAAANFKMPLRSVNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGE3</accession>
    <name>T1GGE3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002462-RA">
        <property type="protein sequence ID" value="MESCA002462-PA"/>
        <property type="gene ID" value="MESCA002462"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087567_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13418" checksum="EDA81553454FFD6D" modified="2013-10-16" version="1">MRKELNAIEENEIDFDEKIVENIIGAIEGQGMFVLFLLINSSDISLENIGDHSFDHDYEGFSRPKNRCLSPSPIRFREDIDVIHVLFVSEALFALNLNWLYYGCITATQLPPLEGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH04</accession>
    <name>T1GH04_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02167669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002686-RA">
        <property type="protein sequence ID" value="MESCA002686-PA"/>
        <property type="gene ID" value="MESCA002686"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1679956_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002686-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17441" checksum="7379593F5BA560B9" modified="2013-10-16" version="1">MVLGFSKRSSTNIISLKAAYLSFALDIASVILVSIFGDGHNSKNWKRPGSATETERRRNLGQTPTGSDGEKDKRYNSLASLSSRSNYYYFDTGIVVPSYLITQVNETTHYFDVVTTHVDNCEMCDRLGDLLLVIVVLYEPDVAVDCCCCKVEVFDAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHE8</accession>
    <name>T1GHE8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02147373">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHE8"/>
    <dbReference type="STRING" id="36166.T1GHE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002844-RA">
        <property type="protein sequence ID" value="MESCA002844-PA"/>
        <property type="gene ID" value="MESCA002844"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1645655_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046098">
        <property type="term" value="P:guanine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051607">
        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002844-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="18710" checksum="0D0FCE9346D89EB2" modified="2013-10-16" version="1">MGLRIRVYGCERMRKERLIGESIVSFATIDLELETNLWLPLEPRNTSSVKMLSNSEQVLIFIFSFFVIAEIGENYTNCKDNVEVIQLNKQQFIMPGFIDTHIHAPQFAQNGLGLDMELLDWLNTYTFPLESKFADKDFASKIYKNVIGKSKFYENTTFFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI25</accession>
    <name>T1GI25_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-gated channel C-terminal leucine zipper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI25"/>
    <dbReference type="STRING" id="36166.T1GI25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003092-RA">
        <property type="protein sequence ID" value="MESCA003092-PA"/>
        <property type="gene ID" value="MESCA003092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266807_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-gated channel C-terminal leucine zipper" evidence="2">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11946" checksum="DC33130DC9A31298" modified="2013-10-16" version="1">MLVENLNVRLARLLAEYTASQAKLKQRIAKLEVNHISFYPPFHLISVSKWNSFLRSCLGLTLYKDLTFHIAICKSLSIMFPDRYPSIALAFLYPRHPVAFHCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI32</accession>
    <name>T1GI32_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02188485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI32"/>
    <dbReference type="STRING" id="36166.T1GI32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003099-RA">
        <property type="protein sequence ID" value="MESCA003099-PA"/>
        <property type="gene ID" value="MESCA003099"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027239_1_1_1"/>
    <dbReference type="OMA" id="LNCERHT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="109"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003099-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="381" mass="44781" checksum="0F1D4A32AC7A0515" modified="2013-10-16" version="1">MWKEWCQFYQQEESTPRDQVPQCKIDIPNKRGVKKLGIINLKHLVHDKNNVAQKEHEEIPCIPFKLGSLSVDTGNIIPLEKDWSKRFTVVTDYLSENIDQIINFEGKEDDVYVVTMSKSGTTWMLETAWLVLNDFNFEEGSTTLLMDRAPYLEHSQIAENFIQICDTHKMLRELKSPRLIKTHLPTHLLPTDLWKKKSKVIYVARNFKDALVSRYRFYNGMGMVSNPMSLEDFAELMMNNKTPYLPYWDHVLEFWELRKQPNIFFTSYERMSKDLRSVIKELCVFLNKPEPSESTLDKAQEYLNFKNMKNSLAGKLHQEAMDKEKVLNCERHTNKAVDFEFMRRGETGSSKDEMTDNLSRKLDQWAESYLQEAGVTVDELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI82</accession>
    <name>T1GI82_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003151-RA">
        <property type="protein sequence ID" value="MESCA003151-PA"/>
        <property type="gene ID" value="MESCA003151"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_937773_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003151-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="297" mass="33665" checksum="9CB75A724029CCBF" modified="2013-10-16" version="1">MACPSQALRTDHVSYRWVVVPYSPFSIDTNRPKNDAEHLPFKSSEIYDIFVLYGLEIGKIRDFHVENSSGPQPNTGEMSRSLPLNIDTSEALQSTHTKPIKVIASTVLPIKTIDSFKSDSILPPILNENGFAQYTDIDSDEKDQETSKENDRHHTDFFFNWHMHKHGQEFRQNDDISRIEPIAKPNLDLITIAALSDLQQPLLVGVIVGSIFLAVILVLYILMLRSQSAEGPSDKSILIAEEENRNNNVVLQSTTVDIPEPCSSQTCHQHVLKQKHNASIFLNFINEIIFCFFMMIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIC0</accession>
    <name>T1GIC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003190-RA">
        <property type="protein sequence ID" value="MESCA003190-PA"/>
        <property type="gene ID" value="MESCA003190"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909065_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003190-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="14752" checksum="2E3FB1FF0D4DE583" modified="2013-10-16" version="1">MSRQQQLMLHLNSLNRSGNNGGVGVGVASLQLEENCYICPQCFRSYRRHGTLRRHLRQECGKGKSMVCSVCGHRTKRADHLRQHVRKRHPELGMRILFNNGNNAPTTAATTATDVNSVSTTAAPAETLGYFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIG0</accession>
    <name>T1GIG0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02028604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIG0"/>
    <dbReference type="STRING" id="36166.T1GIG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003238-RA">
        <property type="protein sequence ID" value="MESCA003238-PA"/>
        <property type="gene ID" value="MESCA003238"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_814542_0_0_1"/>
    <dbReference type="OMA" id="CFCSANF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000592">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000594">
        <property type="entry name" value="cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="124"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="164"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="5">
        <location>
            <begin position="201"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003238-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="37283" checksum="429121B3944B64FC" modified="2013-10-16" version="1">MSRKNMAINKLLQTGDKQVIVGYSPCSQLNVCSNGGVCHEDFYVDVDMKLSIIESESLIITSPYVQHRAICKCSDGYTGNNCEKRQDPCSPNPCKAEGLCRRFGHDFQCQCPPHREGKICQLEKEDVCLGNPCKNGGSCRESPDGSSFFCLCRPGYRGNQCEHIADSCRPNPCLHDGICISATPGYKCSCVGGRYGRHCEKTTYGFQELSFMSFPPLDAATNDISIVFATTKPDALLLYNYGVQTGGRSDFVAIELLKGRAYFSFGGARTAITTIVVGGGGSNNQLESISNGKWHKITATRNGKVMSLSVSKCIENEMFVRNADLGIVHAMLKTLVQLDWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIH1</accession>
    <name>T1GIH1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003250-RA">
        <property type="protein sequence ID" value="MESCA003250-PA"/>
        <property type="gene ID" value="MESCA003250"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640976_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577399" evidence="1">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003250-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="9010" checksum="5AEE1BF9C8C334E2" modified="2013-10-16" version="1">MKFLISVLVAVGLVISHTYAAISFGYDDDLSKYGPRSDHRGAGLSYNGIQYPVYEPNNRVYRSQRVLYNYNRFRRYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJB1</accession>
    <name>T1GJB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 17">Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <shortName evidence="17">GNT-I</shortName>
            <shortName evidence="17">GlcNAc-T I</shortName>
            <ecNumber evidence="14 17">2.4.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 17">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 17">Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11456"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="2.4.1.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="17">The cofactor is mostly bound to the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Golgi apparatus membrane</location>
            <topology evidence="1 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the glycosyltransferase 13 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.101" evidence="14 17"/>
    <dbReference type="EMBL" id="CAQQ02393478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJB1"/>
    <dbReference type="STRING" id="36166.T1GJB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003556-RA">
        <property type="protein sequence ID" value="MESCA003556-PA"/>
        <property type="gene ID" value="MESCA003556"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929960_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003827">
        <property type="term" value="F:alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004139">
        <property type="entry name" value="Glyco_trans_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052261">
        <property type="entry name" value="Glycosyltransferase_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468:SF0">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1/ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03071">
        <property type="entry name" value="GNT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 17" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003556-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="15739" checksum="E2A0B123B7B06742" modified="2013-10-16" version="1">MLSKTIWAELSLKWPKSFWDDWIRHPEQRKNRVCIRPEISRTRTFGKIGVSHGQFFDKHLKFIHLSDANVNFSKLNLNYLLKENYDNTFLKNVYTLPVVTFEELRRNMIKMDGPVRIQYNTKDQYSVLVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJD5</accession>
    <name>T1GJD5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJD5"/>
    <dbReference type="STRING" id="36166.T1GJD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003581-RA">
        <property type="protein sequence ID" value="MESCA003581-PA"/>
        <property type="gene ID" value="MESCA003581"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344636_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577416" description="Carboxylesterase type B domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="33"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="23253" checksum="CBB91E796C89B1C0" modified="2013-10-16" version="1">MELKFTLIFQLFGYFISSILAARDAPPIMIPGQGTVMGTYLKMIRTQNVKAYLGIPYAQAPRFAPPVIGGNEWKGVRNATKFGPDCWQNPKQQLKKHSELFLNLLKESHPVDESSKKFDEECLNLNIFIPDENDLIPYQLESSERHGPFFESILDENIVATMRKPWISPKSKSEFATEKFVFSLLFIVLQFASEGARTRISNNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK91</accession>
    <name>T1GK91_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02118856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003910-RA">
        <property type="protein sequence ID" value="MESCA003910-PA"/>
        <property type="gene ID" value="MESCA003910"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064131_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003910-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13501" checksum="BE10152777BECC22" modified="2013-10-16" version="1">MGSHKTSLLLKSPQIQLETENLVVRNKDGLRYVEDDIKLLNWKEQLKIEVPGENLSSKRNLPEKSKSRGHLLKKVHVAYSQQFNGNFGHGSWESSTSPEIASLPFFELLKSPHPQAIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKJ6</accession>
    <name>T1GKJ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKJ6"/>
    <dbReference type="STRING" id="36166.T1GKJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004022-RA">
        <property type="protein sequence ID" value="MESCA004022-PA"/>
        <property type="gene ID" value="MESCA004022"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2040741_0_0_1"/>
    <dbReference type="OMA" id="VTTKHED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004022-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="14066" checksum="B84BE6EB375E5CF8" modified="2013-10-16" version="1">MAASQNVFQRLVRRMTRFFVTTKHEDTIRILSAELDKLGYTHKLNDDNVMTISTIDRRKLRLVFKAHIIEMDGKILLDFRLSKGCGLEFKRRFMKIKQNLSNVVMKGPITWPIAIATNCVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL42</accession>
    <name>T1GL42_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004231-RA">
        <property type="protein sequence ID" value="MESCA004231-PA"/>
        <property type="gene ID" value="MESCA004231"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827527_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004231-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15904" checksum="60A7F0671C590763" modified="2013-10-16" version="1">MSLTISLESTVSLGGIVLKAYHNDFQHQVFLVLCSDSDVFSQIFKKKPPIPAKPDHLVQQQQAISGILKGGKLWKSEHSSSQLVKCSVHSNTYGNQSYEKNPESIHGMMFQYKVLEKYHEFEYSASISKSLNVPETIEEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM16</accession>
    <name>T1GM16_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM16"/>
    <dbReference type="STRING" id="36166.T1GM16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004584-RA">
        <property type="protein sequence ID" value="MESCA004584-PA"/>
        <property type="gene ID" value="MESCA004584"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_3_1_1"/>
    <dbReference type="OMA" id="FEHYHEE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004584-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12847" checksum="78AB4F459C8441C2" modified="2013-10-16" version="1">MGDVMKEAAFSLAEAKFATGDFNQVVLQNVTKAQIKIRTKKDNVAGVTLPVFESYQDGTDTYELAGLARGGQQLAKLKKNYQSAVKLLVELASLQTSFVTLDDVIKITNRRVNAIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM25</accession>
    <name>T1GM25_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02138149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM25"/>
    <dbReference type="STRING" id="36166.T1GM25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004593-RA">
        <property type="protein sequence ID" value="MESCA004593-PA"/>
        <property type="gene ID" value="MESCA004593"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="OMA" id="MEYLEMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF203">
        <property type="entry name" value="CYTOCHROME P450 4D1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004593-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35768" checksum="2CFA2002BECFF379" modified="2013-10-16" version="1">MYGSLKRFDILFYFFPAYYKLQKQLSVFRKYMKKFIIIEERREYIQDSLNIDESQNNSFYAKRKLAFLDNLLTAKIDNKPLEYKDILDEVSTFLFEGHDTTASAVSFLFYSLSRHPEVQEKLYKEQMSLSSDPNWEPTYEEIQEMKYMELTIKESLRLFPSVPGIGRTPQTDLDINGQKVPAYSTIIILLMAMGYNEDYFDKPCEFIPERFIADNRTGANPFDHVPFSAGPRNCIGQKFALMEVKAIMCQIIRNFKILPPVDGLKTNGIFDISSENRNVGRTKWDPLMSAALTLKSCNGIYIRLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM30</accession>
    <name>T1GM30_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">REM-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104209">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GM30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004598-RA">
        <property type="protein sequence ID" value="MESCA004598-PA"/>
        <property type="gene ID" value="MESCA004598"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631250_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051497">
        <property type="term" value="P:negative regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047138">
        <property type="entry name" value="RHPN1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031:SF15">
        <property type="entry name" value="LD12055P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031">
        <property type="entry name" value="RHOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="REM-1" evidence="2">
        <location>
            <begin position="117"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004598-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18591" checksum="26B40791C62BA5D6" modified="2013-10-16" version="1">GSDPRAATCRGKLQNRRCKLNQEINKELRLXNAGFVNIMIAVSMFVSSPFENHPIQKTETHVNIRIFEIKVHLLRTNELVSLDYDTRVDVLFHSALVVLQVIEFSFNFFLGTSYGSDPRAATCRGKLQNRRCKLNQEINKELRLRAGAENLFKNTNGQSKVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMK5</accession>
    <name>T1GMK5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMK5"/>
    <dbReference type="STRING" id="36166.T1GMK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004780-RA">
        <property type="protein sequence ID" value="MESCA004780-PA"/>
        <property type="gene ID" value="MESCA004780"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1350264_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19744">
        <property type="entry name" value="bHLH_TS_dAS-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF106">
        <property type="entry name" value="ACHAETE-SCUTE COMPLEX PROTEIN T5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="3">
        <location>
            <begin position="35"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004780-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="23281" checksum="CABF7E97176A9483" modified="2013-10-16" version="1">MYSYYNQQELSFEQPMLERSLAKSKKAMYSGEQPMSVIRRNERERNRVRQVNDGYVTLREHIPQFIVSALSNGGRGASKKLSKVDTLRLAVEYIRRLQDAITDYDSDASSTQSSVCGSPEPNMFYQSKTTVTTPNTRNPQAPQLHLCYPRAPAMLTISNNNIRKLSNHSSTNKCTIAASMNHPRMRNYWIALVTGKTNRWRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN67</accession>
    <name>T1GN67_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005010-RA">
        <property type="protein sequence ID" value="MESCA005010-PA"/>
        <property type="gene ID" value="MESCA005010"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870168_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7773" checksum="7CE4630399C9F5F2" modified="2013-10-16" version="1">MSYFWYLVFGAMFIWVLAEVLSIFIKSEDIKIRDPKLFAPWMRKSMKNKKIFDEDLKEPLKVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNS5</accession>
    <name>T1GNS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIF4G-like type 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNS5"/>
    <dbReference type="STRING" id="36166.T1GNS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005234-RA">
        <property type="protein sequence ID" value="MESCA005234-PA"/>
        <property type="gene ID" value="MESCA005234"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725113_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005234-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8620" checksum="35FDBA086CF88C35" modified="2013-10-16" version="1">MILSEHLVRSDTDCRDYDTDWYRWTIGRLQQVFLMHHEQVQKYSSTLETLLFTGDIDSHILDVFNQFVALRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNS7</accession>
    <name>T1GNS7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02059373">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005236-RA">
        <property type="protein sequence ID" value="MESCA005236-PA"/>
        <property type="gene ID" value="MESCA005236"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888321_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005236-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7068" checksum="179542AF6D6F2CB2" modified="2013-10-16" version="1">MQLLTNVCISILGFCLELSEILIIHYLSIMSVKFQSGSMNAFVELMLFARNSWNTGPISVEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNT1</accession>
    <name>T1GNT1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">eIF-4F 25 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02200046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNT1"/>
    <dbReference type="STRING" id="36166.T1GNT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005241-RA">
        <property type="protein sequence ID" value="MESCA005241-PA"/>
        <property type="gene ID" value="MESCA005241"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043552_1_0_1"/>
    <dbReference type="OMA" id="EEFWAIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005241-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="269" mass="30707" checksum="B7E1743686A0F1EA" modified="2013-10-16" version="1">MFPVMSSSSMHPNYNNNGGYHSRKQYHQNGGPHSSNSQYSMVHYQQPPLPPPPQPHYCVNHYPHEPREIIQEDINGHLVVPEALAPDCLIKHPLQNQWTLWYLEADRSKSWEDLQNEISTFDTVEDFWSLYHYIKLASEIRPGSDYSLFKKGIRPMWEDEANKNGGRWMITLNRAAHSDIDNLWLDVILCLIGESFEHSDQICGAVINNRSKAFKISIWTADAKDEEGCLEIGTKLSDALLLDAGALNYQQHSDSMSKQGPGVKPLYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPB7</accession>
    <name>T1GPB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydroxylysine kinase</fullName>
            <ecNumber evidence="8">2.7.1.81</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(5R)-5-hydroxy-L-lysine + GTP = (5R)-5-phosphooxy-L-lysine + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19049"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57882"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58357"/>
            <dbReference type="EC" id="2.7.1.81"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aminoglycoside phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.81" evidence="8"/>
    <dbReference type="EMBL" id="CAQQ02179891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179892">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPB7"/>
    <dbReference type="STRING" id="36166.T1GPB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005442-RA">
        <property type="protein sequence ID" value="MESCA005442-PA"/>
        <property type="gene ID" value="MESCA005442"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042971_1_0_1"/>
    <dbReference type="OMA" id="AAHSCQL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047992">
        <property type="term" value="F:hydroxylysine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1200.10:FF:000007">
        <property type="entry name" value="hydroxylysine kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050249">
        <property type="entry name" value="Pseudomonas-type_ThrB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21064">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21064:SF1">
        <property type="entry name" value="HYDROXYLYSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005442-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="286" mass="32457" checksum="B6D9998B0D929916" modified="2013-10-16" version="1">NCISRVVKNPLITNNWPHGYILKIVNSHDSQKTSLFEAQNEALIYLRNQKIECPNPVANVYGKYFSVENINGVNHIVRLLEFIPGKMFHQVPKTNYLLFSSGEYLAKLDRALKTFHNDVYLTYRTNWMLHEVPKIKDFLSALQDHGRKLIVEEVIGKFETEVMAHIDDFDKQIIHGDYNEHNVIVGPVQPNSEEYKVTGVIDFGDASLSPVIFELAIAVTYMILEAKDLAAGGIVIAGYTSVKPIPKEDLKYIKTCVAARLCQSLVMGAYAYSSSQETTMSWRLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPD3</accession>
    <name>T1GPD3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122506">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPD3"/>
    <dbReference type="STRING" id="36166.T1GPD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005459-RA">
        <property type="protein sequence ID" value="MESCA005459-PA"/>
        <property type="gene ID" value="MESCA005459"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031680_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048619">
        <property type="term" value="P:embryonic hindgut morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002571">
        <property type="entry name" value="Protein odd-skipped-related 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000311">
        <property type="entry name" value="protein odd-skipped-related 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF0">
        <property type="entry name" value="PROTEIN BOWEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005459-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="28604" checksum="3124AFAC8C61F25B" modified="2013-10-16" version="1">MPTEGLTVIDERTISLVRTTRIDQFLQSQAASVASDGSPSGSSSSGSGEGLAEVHPLDGYMIRLAAAAGLLNGPPAGAPHHHRESSAFVPVLPSSNPLRGPPPHGPPGLYGLDGSDKDVASKRGYNNEILAMMADKRKELALRDAAAAAMLLPRQGPNAPPPGLYPHGAGFLGGPVPSPTGPGTFAFPGGTPGLFSAAGVTGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPG3</accession>
    <name>T1GPG3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005493-RA">
        <property type="protein sequence ID" value="MESCA005493-PA"/>
        <property type="gene ID" value="MESCA005493"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_829740_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005493-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="335" mass="38115" checksum="BCFB349607BCC903" modified="2013-10-16" version="1">MESNAPTPIIYKRRERFLDEELDDTKDVITSSSTTPFTPTSQSCNILDSISVKDEDTNCIKNIDENMSKTDSEKLREIAAADKQQQQQQNQQPQLQSIESDRKLTNSFLEQQYKNFVTSEQHQQLLKASFNYDILNFNESICKNSVNSKSCTQSSSTSEAIDFEETALLRRQQLNRVAEWVENNSKIEEQEQNLKNLTLGLQDVNANPQTLNGIISGHIKQTLPNGVRLLTSNFKRTPYHHSKQPQQLQPKLSDEPDIAQTNVILNCKEEDLSSSETQHQEKPDIDLAQMEYNVKQFLLKQNEWSIRSSSNFLGSLDDAMTTAESTTSNATVTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPQ0</accession>
    <name>T1GPQ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPQ0"/>
    <dbReference type="STRING" id="36166.T1GPQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005586-RA">
        <property type="protein sequence ID" value="MESCA005586-PA"/>
        <property type="gene ID" value="MESCA005586"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179532_0_0_1"/>
    <dbReference type="OMA" id="CQRINAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005586-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11815" checksum="B9EFE98F61B44BC9" modified="2013-10-16" version="1">MASLIYTVWSITNVASMHCAARPELSPCTCELANINDVQLTCEKVESFHQIVDALKGKFQPNVTISLTIAHSQLDDLEMRSFSEMNLTVKKLKLQFNNLSKNDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPT8</accession>
    <name>T1GPT8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPT8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005632-RA">
        <property type="protein sequence ID" value="MESCA005632-PA"/>
        <property type="gene ID" value="MESCA005632"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_641409_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005632-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="48829" checksum="3A5102EA2244E17D" modified="2013-10-16" version="1">MSIACFWSNLLIKFIYPRMDITSNARIEKYFGKVKHNIMKGNLNQRPTNFIRLLEEDILSTVRQIDLGFEDVFATKKGLENAKRREHSRQYIFNYEGLESEESCIPENLEQCRDIWRSKYHPKGGLFSHLFQKGVSNKDRAILPFQEKSYASIKEIINVNDVEIREISSIDVVPSKITPVEYEHPPLIDKEIRIDADSSNDCLLKYKNRIPILKDEQLNSLQIHPDHKFINYELISLEGKTKFFIGKIADLSVIGLDIQAITAEMLAVLNPDIWLDADTIAILIGLYIERSGQRNVRLVSSYNIKYLLEPGKIQSVSRNSGLAFILSNTNGIWICPLHTMGSTTPCRTIIDGNHWVLMILDFQAKKFFYLDPLKARSSCAAEVKTTVLSNLKFICSANGKTFDDKNGSTTLGLCTISSRWMASTVDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQA8</accession>
    <name>T1GQA8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retroviral polymerase SH3-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024576">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005812-RA">
        <property type="protein sequence ID" value="MESCA005812-PA"/>
        <property type="gene ID" value="MESCA005812"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1760880_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retroviral polymerase SH3-like" evidence="1">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005812-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="17528" checksum="35495ECCAD5744C8" modified="2013-10-16" version="1">MVEKQTGIAEGNYFHCFMYETKGYRFFEPKSKKVDVSRNASFLENKFYEYATDGKENVFYIFPDVENEVVDQNIVDIQTIENAVFDQNAVDVNGTENLVLGQNTLKEELMHMRYIREPYIFDTYKYICYIHKYACEIDVTYIICLSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQF6</accession>
    <name>T1GQF6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQF6"/>
    <dbReference type="STRING" id="36166.T1GQF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005861-RA">
        <property type="protein sequence ID" value="MESCA005861-PA"/>
        <property type="gene ID" value="MESCA005861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220906_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="24931" checksum="404E56875FC9615A" modified="2013-10-16" version="1">MKYLSSRTIESTIGSDTTTRFGSMRNSSGLNTITLNRYRDQCVWELKSIPSNYFYTLLGLPCIKYFALYAAAKTAVYMKTKHLTRSHAMREATSPPRTPTPRSDRANEFSGSLSNDLGISDSKIHNQLSPTKIMDSGGNAPSVIVTSQNQTNENISNNGSVICEADFPKLTPPKSSKNSRNSSSANSTNTNTKEQSNPQNSQDSSSNRSENSNITQKNDVLLSLIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQJ8</accession>
    <name>T1GQJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha-galactosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02394817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQJ8"/>
    <dbReference type="STRING" id="36166.T1GQJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005907-RA">
        <property type="protein sequence ID" value="MESCA005907-PA"/>
        <property type="gene ID" value="MESCA005907"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013093_0_0_1"/>
    <dbReference type="OMA" id="NIIDWFF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000032">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035373">
        <property type="entry name" value="Melibiase/NAGA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF86">
        <property type="entry name" value="ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17450">
        <property type="entry name" value="Melibiase_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha galactosidase A C-terminal" evidence="8">
        <location>
            <begin position="250"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005907-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="365" mass="41688" checksum="6FCE4F76C08BCECB" modified="2013-10-16" version="1">MTDLVINEGYAAVGYEYINVDDCWLEKSRGLHGQLVADRRRFPSGMRALSDYRAQVWNLSGFRKLYLCWLPGIVGHAQQDADQFADWNVDYVKLDGCYSLPIDMDIGYAEFGRHLNNTGRQMIYSCSWPVYQIYAGLNPNFSAIIEHCNLWRNFDDIQDSWASVESIIDYYGNNQDVIAPNAGPGHWNDPDMLIIGNFGLSYEQSKTQFALWAILAAPLLMSVDLRTIRPEFKAILQNRKIIGVDQDPLGIQGRRIYKHKGIEIWSRPIAPIYQTYYSYAVAFVNRRTDGTPSDVAVTLRELGLTSPTGYRVEDLYEDVDYGVLSPQTKIKVKVNPSGVVILRADVQPERFNRTPYNPLSYFHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS1</accession>
    <name>T1GQS1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS1"/>
    <dbReference type="STRING" id="36166.T1GQS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005985-RA">
        <property type="protein sequence ID" value="MESCA005985-PA"/>
        <property type="gene ID" value="MESCA005985"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_0_5_1"/>
    <dbReference type="OMA" id="KVWRDGG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="33"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005985-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15452" checksum="C0D9DBA21ED546B6" modified="2013-10-16" version="1">SGLPPTVLRDVPFSGIYWSCYESIKGYFNVTTPTFQFSFLAGATAGMVAAFVTTPFDVIKTHRQIEFGTPVKDLPKRKTMDSLARLYSSNGLPGIFAGLGPRLAKVAPACAIMISSFEYGKSFFYRYNEKAYYEKQVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQX0</accession>
    <name>T1GQX0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQX0"/>
    <dbReference type="STRING" id="36166.T1GQX0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006036-RA">
        <property type="protein sequence ID" value="MESCA006036-PA"/>
        <property type="gene ID" value="MESCA006036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009576_0_0_1"/>
    <dbReference type="OMA" id="AHRSEQM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11793">
        <property type="entry name" value="SH3_ephexin1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047271">
        <property type="entry name" value="Ephexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845:SF5">
        <property type="entry name" value="EPHEXIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="390"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="729"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="823" mass="93991" checksum="A38883A14C648980" modified="2013-10-16" version="1">MICRERQDILSWIPNEYISNNSKSLADSQYQNDSGVDMSNIKLPELPGPPTPTSNLYSTLKKLKNFVTNKKSPKSKNKTYESTQFYVPSSTSASTPQLNLQSPDSDPNNCYENVNFETSKSTEKIKPKESNRIKSKLRKSLVIFDSNSNLSSPSEGKSTFYTTGSESSQNLTSNESIIPKKFPTLVKKNKNSKTQRRKTSTDISVPSPEEPIYANKMKSSTSWYTECGVFKESLPNITVTPTMSEERSSNGHTSWYAEAGLYQSNGSRAHQLTVLVAFPRLEMPTHTKMPKMCSQMSHYIKFTMPPKLNLLPEIWKMKGIVHRLMVMRKLVEELTTTMHRQCHLCDGKVGLQLYNWSVRSRDLRGRFGVKFLKSNPVKFYVITLTANERKVQEAKFEIVTSEASYLKSLNLLRSHFMNHPALRDSSVLSSSDRKKLFGLISPVIECSNAFLKALENCWQENIMLLDISKIIYESADKHFHVYIKFCEHQAEMDRTLRRLKENKNGVFNKTLEVLESDATCCGLTLHSFLMLPMQRITRLPLLVDAVFSKIHYASDEHECWKMTLAMLNKFAQQCNDAATKCEQAYEMQRLSSQIEFPSHIAPIPINPTGILSPGTKPDMLSRLGNCCMCDLLVLTKKKTDEQYSVFDYCARSLITISSGEEMLQHLNTGKDTTLIGKNLILLTLLENHEKKTVEMYLSCKTVSEQQRWLQAINPSRESEIPGEKLYEDWDCPQVTVKHEYSSTEPDALNLEPGDIVNVFRKLPDGWYHGERIRDGACGWFPGSYTEEINQLHVRSRNLKQRHRLLTFTAAYLDSQKKQIARVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR20</accession>
    <name>T1GR20_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR20"/>
    <dbReference type="STRING" id="36166.T1GR20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006091-RA">
        <property type="protein sequence ID" value="MESCA006091-PA"/>
        <property type="gene ID" value="MESCA006091"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037828_2_0_1"/>
    <dbReference type="OMA" id="CTYHTTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043988">
        <property type="entry name" value="CCZ1/INTU_longin_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19032">
        <property type="entry name" value="Intu_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU second Longin" evidence="1">
        <location>
            <begin position="120"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="2">
        <location>
            <begin position="271"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="43177" checksum="7BB996BFA2E74D11" modified="2013-10-16" version="1">MILNVPNEVSAKDGAQVSDYRGEEVNDEVYRNVLKQSYKMFKFFNGTFKQNFTGKSKDEQSQNLSNILEQYFKPYLENLKLPTCNYFNILKSVQYFPLEKNLFLRLHNFIKIVKATFNTIDHCAYFFNEDVVWSEICSEDLFCLHQYLTSSLFPKAIQQEISSGAVERNYLIDCDRYGGFLTGQSNSENTIPPPVLYLHEKDGTLERYSLIVYRSMNGTFCIMIDASKDLPNDFYSELQSFMSQQIAKLSSDIGEFFLKSSPVTPITDVDSPRYLFINDLTQQHKGNLTKKTELPLNISNTIAELFSESDSETGKYHEIMVKSTNDYWVVQKSSNHRQFCVVIHSNKSTLIDISNDVNKLFTQEIKNNVFFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR68</accession>
    <name>T1GR68_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA006144"/>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="29" mass="3199" checksum="05D255192486BB21" modified="2013-10-16" version="1">MDFNVKEIRMGVDNIAQGNSVISLFISIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRH5</accession>
    <name>T1GRH5_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Humpbacked fly</name>
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            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="MESCA006260"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006260-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11554" checksum="D6E76B89B2EE80F8" modified="2013-10-16" version="1">MPRQHLIALQSGIRGKIMSCCDRFLLDDDDSVQQKTLMPKLLVFRSSSFSRTSCGLWVFVRKSSLVCLSDVEFRFPKTSFITYDGLLFIRMSSPPWLITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRN1</accession>
    <name>T1GRN1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA006317"/>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588461" evidence="1">
        <location>
            <begin position="19"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="8006" checksum="E14EEFBB3727921B" modified="2013-10-16" version="1">MKFKIFIWLHLFYVAASSDEYHVKIETSVPPLVNSTLTFTATLYHNNKIADPYDYHFKWQDSKGHSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS05</accession>
    <name>T1GS05_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006452-RA">
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        <property type="gene ID" value="MESCA006452"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1724393_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="152" mass="16999" checksum="BBDFB765FC312A37" modified="2013-10-16" version="1">MDDCVISKALLSFCDIALIVETSIDHVLVAFDAMGGGQDPFLSMMEPPQLWIYLSYPLLILTMYLAAPSSAFSPRITRPPFMTIPPSFGKPSIPTRCIKFDFRIYADQINHNIPNPIIHNTICSAINHIHEDSVRTAMAMSKDNRVLGARFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS53</accession>
    <name>T1GS53_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phospholipase A2</fullName>
            <ecNumber evidence="3">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphatidylcholine 2-acylhydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
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        <lineage>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblMetazoa" id="MESCA006501-RA">
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        <property type="gene ID" value="MESCA006501"/>
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    <dbReference type="HOGENOM" id="CLU_118255_1_0_1"/>
    <dbReference type="OMA" id="WFIAPNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PLA2_bee_venom_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12253">
        <property type="entry name" value="RH14732P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
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    </dbReference>
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        <property type="entry name" value="PA2_HIS"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="27"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="50"/>
            <end position="144"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029903"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006501-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="20182" checksum="FE8C04FA9CA0127C" modified="2013-10-16" version="1">MFVKNAGKLTATTLMLFALTLSVASARREKKQVMQAPSDNQDSSLTDWFIAPNTRWCGKGNIAGKYQDLGGASTADKCCRKHDHCKLSIPGLSSKWGLFNYRPFTLSHCSCDMRFRTCLKMANSVDSNMVGNFFFNLVTTECFILKAEETCVMHDPETNECLKTVKKRRAYIRHNKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSE6</accession>
    <name>T1GSE6_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006601-RA">
        <property type="protein sequence ID" value="MESCA006601-PA"/>
        <property type="gene ID" value="MESCA006601"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239694_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006601-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11916" checksum="A89C19D3F3645FBC" modified="2013-10-16" version="1">MSSALLEMTLQNTWSCVKALGRVTEKFQTLRGFCQGDELSWSFFNTLVEMILRAAEIETKNIYLHLSGYFYGLSSKIILETFLAKNTFKSFAVKARTPIHKAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSG6</accession>
    <name>T1GSG6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Down syndrome cell adhesion molecule-like protein Dscam2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02177399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSG6"/>
    <dbReference type="STRING" id="36166.T1GSG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006624-RA">
        <property type="protein sequence ID" value="MESCA006624-PA"/>
        <property type="gene ID" value="MESCA006624"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001038_3_1_1"/>
    <dbReference type="OMA" id="RENASHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056754">
        <property type="entry name" value="DSCAM/DSCAML_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF73">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25059">
        <property type="entry name" value="FN3_DSCAM-DSCAML_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="5"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="82"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="192"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="305"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="394"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="480"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="581"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006624-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="731" mass="82296" checksum="5D116BF5509B8FE2" modified="2013-10-16" version="1">MCFGPSVSIKKSQVAVRRGDRVTLVCEADGDRPLNISWKYRGMRIDPKYDIKYHIKQSEIRVNEYGRDQGNIYVQIQEPPNFPQNLRVLELGSRVLTLGWQPNEMNNAIQNHEFRDAQPITNYILQYKESQDVWHEHNTKKLLKGDKTTTQVEGLKPSHNYHFRLYAENHLGISAPSDILHVQTDSEIPSMPPKSIVAEAITSQQILLTWIPPDRDSWNGELLGYTIGFSRFGSSQKPSYNFTKISVTAASGSNGASEHVNKFRLTDLEKYTQYSIKISAYNVKGDGPYSEPIIAYTLEDVPSTPPQNVMCEALTAQNIQVSWQLPPKDNLNSWDDSNGGSNRETKITSSLNTVLHGLQPFTNYSIQVLAFTKAGDGVLSAAVNCITKEAVPDAPERVKTVVNSESSAIISWLPPKHPNGVVTKYHVYIRILDKGQELKILKETLPAYNTHFEVKDLTPRETYEAWVTASTQTGQGPSTPVIKLTPSNTVPAAIISFGQTLSVSWKVDVKLSCFFVGIPKPSAEWKVINTRAKKHSRLEVSSDNVLILRSVQRSHEGNYSCVVRNNLGTDQIIYNLLVQVPPSPPELFLTSHTPNSVSLDWNVKDNGGAPLRKFLISYRKEFSDWEEIELDHRMHSYVIDKLQCGTQYQFTISAINKIGTSETSPIEFIRTKGRKPIAPQKHFFIRPNVTSVLLELTSWQDGGCPILFYTIEFRKYGAKDWIVVSSSVVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSI3</accession>
    <name>T1GSI3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02081894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSI3"/>
    <dbReference type="STRING" id="36166.T1GSI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006643-RA">
        <property type="protein sequence ID" value="MESCA006643-PA"/>
        <property type="gene ID" value="MESCA006643"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822051_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053:SF24">
        <property type="entry name" value="TRANSPOSASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006643-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="338" mass="38716" checksum="CD1FF57E724FEB0E" modified="2013-10-16" version="1">MKPLDPKKYLKDLIEELIELSVKNCLKTTIKVGIFIFDAVASAFIRGVVSHNAYRACPKCSTTGSYHGRMCYPALNEKSRTDTNFRNRTDPEHHNEKFREPNSSALEALDVDIVKSCPNDYMHLILLGVTKKFLKMILKRIKFPKNALLRMKLDKLNISNISNSIALAHFYQPSDFCRQIRDLEYINFFKAIGGKLPLQELANRVTEHAEIVAGNLKKQMESKVHYPTYTKNKLSLTPEIIVSNDHKNCWILTEDKEIRKFEKVVTVNGEDLVAGKAIKNKTDYFEIPIKSSILDIFHSNGELQEASPIKFSKIQCKLFCIPDINNDRVFIPILHTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSW2</accession>
    <name>T1GSW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSW2"/>
    <dbReference type="STRING" id="36166.T1GSW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006774-RA">
        <property type="protein sequence ID" value="MESCA006774-PA"/>
        <property type="gene ID" value="MESCA006774"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157491_0_0_1"/>
    <dbReference type="OMA" id="KNTITRW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="33"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006774-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="14077" checksum="F7E7DA1FCF7155FA" modified="2013-10-16" version="1">MTEHKRLNINFEEDEEFDMEAERDLAEDAQWKKIQQNTFTRWANEHLKNIANVANLETDLCDGLRLIALIEVLSQKRLPKHNKRPTFRSQKLENVSVALKFLEDEGIKIVNIEKFLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTG6</accession>
    <name>T1GTG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006997-RA">
        <property type="protein sequence ID" value="MESCA006997-PA"/>
        <property type="gene ID" value="MESCA006997"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624831_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9191" checksum="8882FE82F8928B3C" modified="2013-10-16" version="1">MLEQLPSPTGEQQQKTVCHRWMDVYVCVYKDLIGNIKCIKQAGFSVVFKLTHIPILENWTDYTKVQFTWHLFATPDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTK3</accession>
    <name>T1GTK3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTK3"/>
    <dbReference type="STRING" id="36166.T1GTK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007037-RA">
        <property type="protein sequence ID" value="MESCA007037-PA"/>
        <property type="gene ID" value="MESCA007037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164133_3_0_1"/>
    <dbReference type="OMA" id="IASRLMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF374">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="4">
        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8876" checksum="D2927A179CCFC641" modified="2013-10-16" version="1">NNEGIGLFNSNFSHFLNVTSSIPDRCKLKPAGGHCKASQRRYFYNYKLHKCQGFNYGGCIGNLNNFYSIEDCIQVCMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTS0</accession>
    <name>T1GTS0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02052690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTS0"/>
    <dbReference type="STRING" id="36166.T1GTS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007111-RA">
        <property type="protein sequence ID" value="MESCA007111-PA"/>
        <property type="gene ID" value="MESCA007111"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006480_3_2_1"/>
    <dbReference type="OMA" id="TTIRIHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000140">
        <property type="entry name" value="Dachsous cadherin-related 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000353">
        <property type="entry name" value="Dachsous, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF126">
        <property type="entry name" value="PROTOCADHERIN FAT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="29"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="142"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="207"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007111-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="359" mass="40443" checksum="44CD15C0A5C6E57B" modified="2013-10-16" version="1">RTDGSPLLYGYTEVYVILEDENDNGPHFTQQQYTATVWEGNNKGTFVMQVKAFDADDGANSRILYHIVDGNHDNAFIIEPAFSGIVKTNIVLDREIRDLYSLKVIATDEGIPQMTGTTTIRIHIVDVNDNQPTFPPHKIINVSEVTGVLFVNNKLDYDEGATIYNLIIRACDSGNVSLCTLHPFRIALADENDNNPKFPVHEYIEYIRENEPIDTAIFTARATDLDKGRYGQLNYTIEPSSSMYGSDDAWKLFTIDELSGIVTTNAVFDYEERSRYDFMVKATDIGGKSAVVKVRMLIDSNDEYSPQFTERTYHFVMSPSSISYLPVGYVIGQVTATDRDKGPDGHVVYQLTSQNPYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTX1</accession>
    <name>T1GTX1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007168-RA">
        <property type="protein sequence ID" value="MESCA007168-PA"/>
        <property type="gene ID" value="MESCA007168"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592498_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9321" checksum="9CD7BE4C5D695F87" modified="2013-10-16" version="1">MEVPLQLKKETFESRVKPVLTYGSQARSNPKRNMGKLSICQRNMERSILNIRITDKIRNTAIRNLTGFKDNVKNFNLQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUG6</accession>
    <name>T1GUG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007379-RA">
        <property type="protein sequence ID" value="MESCA007379-PA"/>
        <property type="gene ID" value="MESCA007379"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707624_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007379-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8135" checksum="03D4AE1D2D80EBE0" modified="2013-10-16" version="1">MAVIDFSYADDVVLITRSKFLNTFSELLQSALKVVSVWTETNGLGVNATKTELVLFTNRHRSLNSQTVPNTCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUM9</accession>
    <name>T1GUM9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUM9"/>
    <dbReference type="STRING" id="36166.T1GUM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007446-RA">
        <property type="protein sequence ID" value="MESCA007446-PA"/>
        <property type="gene ID" value="MESCA007446"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_8_0_1"/>
    <dbReference type="OMA" id="IAGNHAC"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051607">
        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007446-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="19886" checksum="9BFE4E595D395DA9" modified="2013-10-16" version="1">MAHGVFLEDEEIKLLAKTGTSVSHCPASNTMLSSGLCDVFRLLNGGVKVGLGTDVSGGNCVSIKDVILRALDVSHHLEFIKKHEIKGTGKMPTTDETYKSMNYKQAIYLATLGGASAIAVDDKVGNFDVGKEFDALFIDTSIFPMPNFVLSELLEQKFTPEQKVLELVQKFIFNGDDRNIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUN7</accession>
    <name>T1GUN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diacylglycerol kinase iota-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007455-RA">
        <property type="protein sequence ID" value="MESCA007455-PA"/>
        <property type="gene ID" value="MESCA007455"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2628949_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diacylglycerol kinase iota-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8827" checksum="878354B7F139AF8B" modified="2013-10-16" version="1">PNYEHYHNDKDLVKASATKVGEIQIVPTADLRQVREAIKKLLEQDQDANTLELGMIGCLLITYPKDIIDTCRKVVTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV19</accession>
    <name>T1GV19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat-containing protein 55 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV19"/>
    <dbReference type="STRING" id="36166.T1GV19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007597-RA">
        <property type="protein sequence ID" value="MESCA007597-PA"/>
        <property type="gene ID" value="MESCA007597"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718227_0_0_1"/>
    <dbReference type="OMA" id="VIANHEV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF21">
        <property type="entry name" value="PROTEIN GROUCHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01850">
        <property type="entry name" value="GROUCHOFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="274"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="316"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="41988" checksum="D00759BC808ACCD4" modified="2013-10-16" version="1">MPQVPLGPFGAAGQLGLPHASNLLKAPQDLHREDIKVPLGGQSAEERLVSIVCEWLAVGMENSHVEVLHASKADKYQLHLHESCVLSLRFATCGKWFVSTGKDNLLNAWRTPYGASIFQSKETSSVLSCDISTDDKYIVTGSVPAGKPAYSFHMNGEGTLQPVPFPPDALMGVGIPRHARQINNLAHGEVVCAVTISNPTKYVYTGGKGCVKVWDISQSNNKSPVSQLDCLQRDNYIRSVKLLPDGRTLIVGGEASNLSIWDLASPTPRIKAELTSAAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISPDGQRLWTGGLDNTVRSWDLREGRQLQQHDFSSQIFSLGYCPTGELSLFYRFIFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV27</accession>
    <name>T1GV27_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02002191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02002192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007607-RA">
        <property type="protein sequence ID" value="MESCA007607-PA"/>
        <property type="gene ID" value="MESCA007607"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388720_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007607-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="11342" checksum="95F91C4F46F3CF05" modified="2013-10-16" version="1">MIRNSQENFYDKRKTVELAKLEKRRFEKDQFEGLDLMRERNQGKTYLEIVRNVDSCSSFFRRALTLLESFKVAQFAREVTSIMMCWGLTYSFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV86</accession>
    <name>T1GV86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucuronosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02135104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV86"/>
    <dbReference type="STRING" id="36166.T1GV86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007673-RA">
        <property type="protein sequence ID" value="MESCA007673-PA"/>
        <property type="gene ID" value="MESCA007673"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012949_8_1_1"/>
    <dbReference type="OMA" id="HNIDAFT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007673-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16520" checksum="C2D9251783499DAF" modified="2013-10-16" version="1">MMSLLIQITTETIYHGVPVIGIPMFGDQFLNMAQAVNGGYGITVDYNLLSVDYLKEIIEEMVTNESYTNKIKAMSKRYRDQPLTPLETAIYWVEYVIRHNGAYHLRNAGMDLNFIQYHNIDAFTILYGIIFIIIFILFKFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB7</accession>
    <name>T1GVB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB7"/>
    <dbReference type="STRING" id="36166.T1GVB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007709-RA">
        <property type="protein sequence ID" value="MESCA007709-PA"/>
        <property type="gene ID" value="MESCA007709"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2580683_0_0_1"/>
    <dbReference type="OMA" id="REMCHIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007709-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9299" checksum="AA0AC497AA4BF727" modified="2013-10-16" version="1">MPEVYPIPNQEARTSSDVSIKKWVSTFGVPIEIQSDQGRNFETTLFSKMWKTLGMNKTTKTTPLHPQSNSQWLTVLLFIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVF8</accession>
    <name>T1GVF8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02130600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVF8"/>
    <dbReference type="STRING" id="36166.T1GVF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007757-RA">
        <property type="protein sequence ID" value="MESCA007757-PA"/>
        <property type="gene ID" value="MESCA007757"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012437_4_0_1"/>
    <dbReference type="OMA" id="GKMSENC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000389">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007757-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="37236" checksum="9C4A5DF04EAF1F08" modified="2013-10-16" version="1">MFAVNLFRTLPPSSNPNGAEFDPEEDEPTLEASWPHLQFVYELFLRFLESSDFQPTIAKRYIDHQFVLNLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNVFYRFIYETEHHNGIADIINGFALPLKEEHKQFLLKVLLPLHKVKSLSVYHPQLTYCVVQFLEKDPSLSEPVIKSLLKFWPKTHSPKEVMFLNELEELLDVIEPAEFQKVMVPLFRQISKCVSSPHFQVAERALYFWNNEYIMSLIADNSTVILPIMFPSLYRNSKSHWNKTIHGLIYNALKLFMEMNQKLFDECNKNYRQEKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVJ1</accession>
    <name>T1GVJ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein BRX1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Brix domain-containing protein 2 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required for biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BRX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVJ1"/>
    <dbReference type="STRING" id="36166.T1GVJ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007799-RA">
        <property type="protein sequence ID" value="MESCA007799-PA"/>
        <property type="gene ID" value="MESCA007799"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048373_0_0_1"/>
    <dbReference type="OMA" id="YRHRHLM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000003">
        <property type="entry name" value="Ribosome biogenesis protein BRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="9">
        <location>
            <begin position="43"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007799-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="36774" checksum="DD55F1B3C51F5717" modified="2013-10-16" version="1">MGKRKVIKKSKTDVVVKEEENVLPSKRNSDEAVPKKEKWINKQRVLVLSARGINHRDRHLMKDLRTLMPHHRPEGKMERTKSLSVLTDICEMKHCNKALLFEGRRKRDLYMWLANCPSGPSVKFLVENVHTMGELKLTGNCLRGTRPLLSFDSKFDEIPHLSLLKELLTQIFGVPNYHPKSQPFFDHVYTFTFLDNRIWFRNFQILSEDGGLSEVGPRFVMNPVKILVDHLLNPDYVSPSKTRQMIKKSAKDKYVSRIEQKSKQDSNQPTEAYEDDGLDMFGGDELDAAKIISQQRTKTISAGKKLKTKKIGNLKSKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWL9</accession>
    <name>T1GWL9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008196-RA">
        <property type="protein sequence ID" value="MESCA008196-PA"/>
        <property type="gene ID" value="MESCA008196"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3399818_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008196-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="31" mass="3457" checksum="2FBB0D7B94C70A9D" modified="2013-10-16" version="1">MSIEEDLMLQQPMLTNSTFSESGAVNILDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWY9</accession>
    <name>T1GWY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWY9"/>
    <dbReference type="STRING" id="36166.T1GWY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008324-RA">
        <property type="protein sequence ID" value="MESCA008324-PA"/>
        <property type="gene ID" value="MESCA008324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_932470_0_0_1"/>
    <dbReference type="OMA" id="DETRDNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030832">
        <property type="term" value="P:regulation of actin filament length"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052409">
        <property type="entry name" value="Myosin-III_kinase_activity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256">
        <property type="entry name" value="AGAP011099-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256:SF2">
        <property type="entry name" value="NEITHER INACTIVATION NOR AFTERPOTENTIAL PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 11" id="KW-0518">Myosin</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="299" mass="34785" checksum="03CB5E8012A450C6" modified="2013-10-16" version="1">FYDNRTALDNLLTKPDGLFCIIDDCTRNNFSDSNMLDQITEKKSQFIKMHSNTEISVAHFTGKIIYDVRNFKDTNRDFVPPEMIESLRTSLDETIVLMFTNQLTKSGNLTMAFENVEHKSDAKRRTYALNTLSVGHISQVNNIRTLSANFRHTCLELLKVLSRGFGYGTHFVRCIRADLEYIPRNYHPEMVAQQMRALGVLDTLAGRQKGYSCRISFSEFLRRYQFLAFDFDETVDITKDNCRLLLLRLKMEGWAIGKSKIFYDEYLSRLYEIQVKKVIKVQSMMRAMLAKRKVKKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ3</accession>
    <name>T1GXJ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibroblast growth factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02386693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXJ3"/>
    <dbReference type="STRING" id="36166.T1GXJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008545-RA">
        <property type="protein sequence ID" value="MESCA008545-PA"/>
        <property type="gene ID" value="MESCA008545"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1134667_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008545-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27700" checksum="3D3FF6A8DF273CD6" modified="2013-10-16" version="1">MIGVPRSKTKSDIQYLTLFSKDTELYICYNKKWRIVGMKNPSERCYFKEGRHFEFISYLNNSMYIGFVCKNGACKSAGPEEKSECFSSTKHEPNVIYDYYRKHNNSHHRIRHRKHHQKHRDKHHLHTTTTITTSTTSTTPTTTTTTSTTTTTPQPARRRNKKKSKLSKKRPKPSKASEFVTAELLSTVYSSSIGYQTETVTPSSPFTVDLDSTSATSGSSSDSSDTSSISSVMDSSSYGTTDTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY75</accession>
    <name>T1GY75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nicotinamide-nucleotide adenylyltransferase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nicotinate-nucleotide adenylyltransferase 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury. May be involved in the maintenance of axonal integrity. Also functions as a stress-response chaperone protein that prevents toxic aggregation of proteins; this function may be independent of its NAD(+) synthesis activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02023163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY75"/>
    <dbReference type="STRING" id="36166.T1GY75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008797-RA">
        <property type="protein sequence ID" value="MESCA008797-PA"/>
        <property type="gene ID" value="MESCA008797"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033366_3_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000221">
        <property type="entry name" value="Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF0">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="15">
        <location>
            <begin position="14"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008797-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="212" mass="24637" checksum="305B91F7A7956ABD" modified="2013-10-16" version="1">MFNSNMGRPKIVLIACGSFSPPTPMHFRMFEIAKDYFKAHETHDVIGGIVSPTHDSYGKKGLVNGSHRINMLRLGLRSSNWIKISDWEIKQDQWSRTKKVLEYHQNFLNNYINSRNNNIDFDENCPEWFPDKSVLQNDLIKVKLLCGADLLESFAVPGLWQDEDIEDILVNHGIIVITRSGSNPEKFIFESDILSKYRVSLKYYYFLILFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU0</accession>
    <name>T1GYU0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMMD8 helical N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYU0"/>
    <dbReference type="STRING" id="36166.T1GYU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009027-RA">
        <property type="protein sequence ID" value="MESCA009027-PA"/>
        <property type="gene ID" value="MESCA009027"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1490227_0_0_1"/>
    <dbReference type="OMA" id="FSHKYGF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055184">
        <property type="entry name" value="COMMD8_HN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22838">
        <property type="entry name" value="COMMD8_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMMD8 helical N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009027-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="20048" checksum="7BE155C4DDABA648" modified="2013-10-16" version="1">MPDLLLTLDNREVFTEILHHSIDFLIKAHPTKTAKSRIYGNSADEYESTIKNISNNYKIFCLKQISEAELLGKYSHMNSDLQKMILPVFKAREQEIKFFLLQEHNSKIGDLVESFDWNVNLIFGDSSLPTHRRIIGKVILNCLDENLKSKQMHFEVDKKKLNEIISVLEGLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYW8</accession>
    <name>T1GYW8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYW8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009055-RA">
        <property type="protein sequence ID" value="MESCA009055-PA"/>
        <property type="gene ID" value="MESCA009055"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500466_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009055-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9935" checksum="91B105FD8AB4B988" modified="2013-10-16" version="1">MRIFLQRTTTTKVEHPLNQSISPRRSKTPKSPSTNKSRESEDLSLYIHGLLSHSVFDSFTLPRFTLPELARGSTMTNWIYLPLFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZB7</accession>
    <name>T1GZB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZB7"/>
    <dbReference type="STRING" id="36166.T1GZB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009215-RA">
        <property type="protein sequence ID" value="MESCA009215-PA"/>
        <property type="gene ID" value="MESCA009215"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1391661_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2750:FF:000002">
        <property type="entry name" value="TBC1 domain family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021785">
        <property type="entry name" value="DUF3350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF16">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11830">
        <property type="entry name" value="DUF3350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="131"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009215-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22906" checksum="85C70270414499DC" modified="2013-10-16" version="1">MVDIFYKVGNSPKEVSAEDKGSFRQAILNSVTASSEEVEPTKIKRTPAEYRELWKQAIRQTILLIRMERENANLLAKQNENEIKKIKLDYDTIIKCDRQLVDRWETIMERHSSKIATKRDPKVLFQAIKNGVPRAKRGEVWTFLAEQHSLHTAPVDTKNFPMYHTPYPVLLKSLTEHQHAIFIDLGRTFPNHVYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZC9</accession>
    <name>T1GZC9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378988">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009228-RA">
        <property type="protein sequence ID" value="MESCA009228-PA"/>
        <property type="gene ID" value="MESCA009228"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3431734_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="18" mass="2093" checksum="163697A2206DC145" modified="2013-10-16" version="1">MELLKNFERQPGKVSFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZL5</accession>
    <name>T1GZL5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009318-RA">
        <property type="protein sequence ID" value="MESCA009318-PA"/>
        <property type="gene ID" value="MESCA009318"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457363_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009318-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="10365" checksum="8D059AF8AE1797F3" modified="2013-10-16" version="1">MTQSEAKRNLGSVTCYLEPKLFKRNLRNNSFVTDELWLPIKDITTTPMKPGRNSVKLFPGSKKYDSKIQTNSWPKFNENFKFPLVPDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0D6</accession>
    <name>T1H0D6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0D6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009611-RA">
        <property type="protein sequence ID" value="MESCA009611-PA"/>
        <property type="gene ID" value="MESCA009611"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760720_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009611-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8628" checksum="DFFF5EE8540B5C8E" modified="2013-10-16" version="1">MGNTRTHPWLKFFESSHLYAKDFRKLHRLSTQTTKFRMSSRYRKLTWKGFPRTLTQKPLFKDTTHLFFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H154</accession>
    <name>T1H154_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H154"/>
    <dbReference type="STRING" id="36166.T1H154"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009907-RA">
        <property type="protein sequence ID" value="MESCA009907-PA"/>
        <property type="gene ID" value="MESCA009907"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1024102_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037689">
        <property type="entry name" value="BAG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12334">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12334:SF6">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009907-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30892" checksum="F934BBFCB007EFBE" modified="2013-10-16" version="1">MEKISKLLGFLRKRKNNDCPEDEVEEEEDFVPPSKKLERNLTVKINKDHTNLELNANLERLSISDVDKFCGKEVFLESCDIPKVDPQPNTTTTTLPGPHIIEAMDIDGVSSSTTTERLTPPREFCDSPYHQHSESSMNFDILDNIMESTEKEEIALYIDRVNSRLATVELNVRTVRDRSQEDSLSQVNALIDAMITAGDPVISRQRCQHYLNSCSDNHSFNLYDGAHSSIDTSIVDKKFESALLGCTLDDQKNIKKRLSALMNYLNKQTIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H182</accession>
    <name>T1H182_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl hydrolases family 22 (GH22) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H182"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009936-RA">
        <property type="protein sequence ID" value="MESCA009936-PA"/>
        <property type="gene ID" value="MESCA009936"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530057_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019799">
        <property type="entry name" value="Glyco_hydro_22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00128">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 22 (GH22)" evidence="2">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009936-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9342" checksum="32B3B0654784D948" modified="2013-10-16" version="1">MYGQCKVLCTDLLSDDLTEDLKCASFIKSAVGFLAWPKYKTHCFEPESSKLCMKVSSTLRISVALICILVPVEIRIPPGYLISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1A9</accession>
    <name>T1H1A9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1A9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009966-RA">
        <property type="protein sequence ID" value="MESCA009966-PA"/>
        <property type="gene ID" value="MESCA009966"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778734_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009966-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7773" checksum="9E4403C959FE3FA8" modified="2013-10-16" version="1">MKGTQFLLSGTFSTPRGLRFASRLKKRTPPGVGQVFGSSLPTKKKNTFLSNEVILYRLRFAPHELAYKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1C5</accession>
    <name>T1H1C5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1C5"/>
    <dbReference type="STRING" id="36166.T1H1C5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009984-RA">
        <property type="protein sequence ID" value="MESCA009984-PA"/>
        <property type="gene ID" value="MESCA009984"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433181_0_0_1"/>
    <dbReference type="OMA" id="LAFIWKH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009984-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10820" checksum="22F25ECE8DAFA6E3" modified="2013-10-16" version="1">MYGRYTKDLGEFAKDEARKLKILEKRRKQEDRARNKELLGKRTSRVSKCFSWIWRHTFARLGEDWVFLALLGIIMAFLSYIMDKGISICTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1H7</accession>
    <name>T1H1H7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TFIIB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1H7"/>
    <dbReference type="STRING" id="36166.T1H1H7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010038-RA">
        <property type="protein sequence ID" value="MESCA010038-PA"/>
        <property type="gene ID" value="MESCA010038"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193165_0_0_1"/>
    <dbReference type="OMA" id="SYGYGKF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000012">
        <property type="entry name" value="Putative transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="11">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010038-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="8480" checksum="0A280D81A623257F" modified="2013-10-16" version="1">MSGVKCKNCGSTDIEVDNARGDAVCTNCGSVLEDNIIVSEVQFEEAGHGSTAIGQFVSADSGGGATSYGYGKFQVGSGHESRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H215</accession>
    <name>T1H215_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02146736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H215"/>
    <dbReference type="STRING" id="36166.T1H215"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010245-RA">
        <property type="protein sequence ID" value="MESCA010245-PA"/>
        <property type="gene ID" value="MESCA010245"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137711_0_0_1"/>
    <dbReference type="OMA" id="WVCFEDI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902850">
        <property type="term" value="P:microtubule cytoskeleton organization involved in mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000996">
        <property type="entry name" value="Cytoplasmic dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF63">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="11">
        <location>
            <begin position="34"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="16930" checksum="10F7429BC9B4E4FD" modified="2013-10-16" version="1">LDAQEFGWESQLRFYWLKQFDNLYVIQCTGSFEYGYEYMGLNGRLVITPLTDRIYLTITQALLMNLGGAPAGPAGTGKTETCKDLAKAMALLCIVTNCGEGMDFRAVGTILSGLSQCGAWGCFDEFNRIDISVLSVISTQLQTIRSALIRKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H349</accession>
    <name>T1H349_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myotubularin-related 12-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H349"/>
    <dbReference type="STRING" id="36166.T1H349"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010664-RA">
        <property type="protein sequence ID" value="MESCA010664-PA"/>
        <property type="gene ID" value="MESCA010664"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1442607_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022587">
        <property type="entry name" value="MTMR12-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12578">
        <property type="entry name" value="3-PAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin-related 12-like C-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010664-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="21830" checksum="864AB642EEA48ABA" modified="2013-10-16" version="1">MLFTNPFFTKKENTVVRKSLAIPPNAIPLHGLQKSFSSSRLLRDHSLHTDSIPKDKFLSPKWEISSLSVWQQCYHRWIPLFEIKNGGFPHIDLFNRLLLSNINKLQKKLESGYDFYGDDEDDEDTINVIGERIERRHKLSSPNNEINFENVNSFFPFSSNENNSDQLNEILISSNDVLMDGSIMERND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1H375</accession>
    <name>T1H375_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02150639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H375"/>
    <dbReference type="STRING" id="36166.T1H375"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010694-RA">
        <property type="protein sequence ID" value="MESCA010694-PA"/>
        <property type="gene ID" value="MESCA010694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109117_4_1_1"/>
    <dbReference type="OMA" id="WISNMSG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000012">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="12">
        <location>
            <begin position="16"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7762" checksum="0690F6F35584AAF1" modified="2013-10-16" version="1">PPPPIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4T2</accession>
    <name>T1H4T2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein C10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0456 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4T2"/>
    <dbReference type="STRING" id="36166.T1H4T2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011300-RA">
        <property type="protein sequence ID" value="MESCA011300-PA"/>
        <property type="gene ID" value="MESCA011300"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144250_0_0_1"/>
    <dbReference type="OMA" id="PLVMQIQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026317">
        <property type="entry name" value="P_C10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13463">
        <property type="entry name" value="PROTEIN C10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13463:SF3">
        <property type="entry name" value="PROTEIN C10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14974">
        <property type="entry name" value="P_C10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13284" checksum="2CB7443F5EE00671" modified="2013-10-16" version="1">MAYLTNFNNEAAREILIDIIRTVNQPENSKKLAEAKGNAGKEMILMMQHVFPLVMQIQTEVIKKHGFPGNREGLVQFAMMVREMEKDDQEISRLRSQIRAIYLPPIAINTTNDVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4T3</accession>
    <name>T1H4T3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02010134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4T3"/>
    <dbReference type="STRING" id="36166.T1H4T3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011301-RA">
        <property type="protein sequence ID" value="MESCA011301-PA"/>
        <property type="gene ID" value="MESCA011301"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340505_0_0_1"/>
    <dbReference type="OMA" id="NTNDFIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000166">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="105"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011301-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23654" checksum="D114AE043D80FF3F" modified="2013-10-16" version="1">AYCYTEGSFCQQWLQAQIEKSQTYDIRPSNTRRNDFIENENSLHQSTSSGITSSNSNVNSHTNTNDFIMNGRGYRGQEYEPMKLKCGIAPALRNNRRNMNNLLKIIGGKASRKGEWPWQVAIFNRYKEAFCGGTLIAPQWILTAAHCLRKVLYVRLGEHNLDFEDGSELQFRVLRSYKHPSFDRKTVDSDIALLNIYTLRFAYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H522</accession>
    <name>T1H522_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02181929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H522"/>
    <dbReference type="STRING" id="36166.T1H522"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011396-RA">
        <property type="protein sequence ID" value="MESCA011396-PA"/>
        <property type="gene ID" value="MESCA011396"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046313_2_3_1"/>
    <dbReference type="OMA" id="GHSQLDW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000001">
        <property type="entry name" value="Cytochrome b5 reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="51"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011396-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15367" checksum="75582FA6E133E956" modified="2013-10-16" version="1">MWAKLGFSNNSTGSATGNPRNKVALKPGHSLMDWVRLGSSGEDLTGANGMIRPITLQEVALHNTQNDAWTAIRGKVYNITRYMDFHPGGVEELMKGAGKEATKIFEDVHAWVNYEQLLAKCFLGPLTIAASLDLGMDTDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H589</accession>
    <name>T1H589_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60S ribosomal protein L36</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H589"/>
    <dbReference type="STRING" id="36166.T1H589"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011464-RA">
        <property type="protein sequence ID" value="MESCA011464-PA"/>
        <property type="gene ID" value="MESCA011464"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064138_0_0_1"/>
    <dbReference type="OMA" id="CTMAPRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011464-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13925" checksum="18A01FE87CDD35FF" modified="2013-10-16" version="1">MQTEILQICFCQNSDWSELFRFAPKIAVRYELCIGLNKGHKTTKLGNVRYTGDKKAQGLRGYRLKNIQRFQRELVSGVCGHYEKKAFLKLRLRTHILVRRKSEELSNILTQMRKTGHAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5B0</accession>
    <name>T1H5B0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5B0"/>
    <dbReference type="STRING" id="36166.T1H5B0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011487-RA">
        <property type="protein sequence ID" value="MESCA011487-PA"/>
        <property type="gene ID" value="MESCA011487"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_3_1"/>
    <dbReference type="OMA" id="GIWINIE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000159">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF19">
        <property type="entry name" value="KATANIN P60 ATPASE-CONTAINING SUBUNIT A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="21961" checksum="F2E0FE1523394940" modified="2013-10-16" version="1">MWMPDYFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKMVRLLFEMARFYAPSTIFIDEIDSLCSRRGSESEHEASRRVKSELLVQMDGVGGQEEASKVVMVLAATNFPWDIDEALRRRLEKRIYIPLPTDEGREALLKINLREVKVDETVDLKSIAKQLEGYSGADITNVCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5F1</accession>
    <name>T1H5F1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02057279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5F1"/>
    <dbReference type="STRING" id="36166.T1H5F1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011528-RA">
        <property type="protein sequence ID" value="MESCA011528-PA"/>
        <property type="gene ID" value="MESCA011528"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149433_2_0_1"/>
    <dbReference type="OMA" id="GMSHNET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11324">
        <property type="entry name" value="IL16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11324:SF16">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011528-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="10813" checksum="017E4F219BA55EF4" modified="2013-10-16" version="1">MWLHNRNTVLTVAFIKGVGMKSLGFSIVGGHDSPKGIMGIYVKTVFPNGQAFDDGTLKAGDEIIEINGISLDGMSHNETISIFKNIREGPVNIKVLRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5F7</accession>
    <name>T1H5F7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02110194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5F7"/>
    <dbReference type="STRING" id="36166.T1H5F7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011534-RA">
        <property type="protein sequence ID" value="MESCA011534-PA"/>
        <property type="gene ID" value="MESCA011534"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075831_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011534-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13285" checksum="8F8D541F5B73A0FD" modified="2013-10-16" version="1">MSEESNNSRFFKVFSKRSSLDLAPDVQKSPDTKRSLCGRILWILVFILSSYICSILVWKSVSILSGDPIQITVDEQLSSIAEIPFPAMTICPQKKIKDNINLVTILEAYFKENITAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5U8</accession>
    <name>T1H5U8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5U8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012081-RA">
        <property type="protein sequence ID" value="MESCA012081-PA"/>
        <property type="gene ID" value="MESCA012081"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1699915_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="3">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012081-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17355" checksum="DE25251641E43B48" modified="2013-10-16" version="1">IKLKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXICDNLLMCTARAWDLSKPLIFCPAMNTKMYNHPLTEEQIEKLKGWGYREVPCISKIVCGDTGNGAMAEVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5X1</accession>
    <name>T1H5X1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5X1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012104-RA">
        <property type="protein sequence ID" value="MESCA012104-PA"/>
        <property type="gene ID" value="MESCA012104"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008187_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000431">
        <property type="term" value="P:regulation of cytokinesis, actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026817">
        <property type="entry name" value="Ect2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049395">
        <property type="entry name" value="ECT2_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16777">
        <property type="entry name" value="PROTEIN ECT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16777:SF2">
        <property type="entry name" value="PROTEIN ECT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21242">
        <property type="entry name" value="ECT2_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="28"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="729"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012104-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1077" mass="121675" checksum="DE925D2ACEC5F798" modified="2013-10-16" version="1">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</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H710</accession>
    <name>T1H710_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H710"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012492-RA">
        <property type="protein sequence ID" value="MESCA012492-PA"/>
        <property type="gene ID" value="MESCA012492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016072_7_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000060">
        <property type="entry name" value="F-box/LRR-repeat protein 20 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF165">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN FBXL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="11"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="397" mass="44488" checksum="D4DA588EAB7012AC" modified="2013-10-16" version="1">QACLNFYDNDDEPIKQLPKEILLRIFSYLDVVSLCRCAQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNLTEIDVSWCNIITVNGVEALARGCKKLKKFCSKGCKQINDNAIICLANYCHDLLILNLHSCEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLSHCELITDDGIRQLAAGNCAAESLSVLELDNCPLITDRTLEHLISCHNLQRIELFDCQMITRAAIRRLKSHLPNIKVHAYFAPATPPPITAGNRQRYCRCCEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H731</accession>
    <name>T1H731_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H731"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012513-RA">
        <property type="protein sequence ID" value="MESCA012513-PA"/>
        <property type="gene ID" value="MESCA012513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096876_1_0_1"/>
    <dbReference type="OMA" id="LWIMFAD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17599" checksum="DF2D4E0BF54D5812" modified="2013-10-16" version="1">MTIIEHIREQFTGENSRNKNSAXISGEFFFFTGWWILIDGLSVDPKHQITTGQAFIGVFGTVSFFMVNTVRNSHLTEENTSENGAMIAKGWLMIGFLMGFASIVAALWVMIADFINNRNRKETKYGVAILMENILIFIANLVYKFGRSEEAWPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7M2</accession>
    <name>T1H7M2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7M2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012759-RA">
        <property type="protein sequence ID" value="MESCA012759-PA"/>
        <property type="gene ID" value="MESCA012759"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140398_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012759-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="244" mass="27548" checksum="65972349CAF52185" modified="2013-10-16" version="1">NPQMRSSTTNLLIANLAVADLLFVVFCVPFTAADYMTRQWPFGLYWCKIVQYLITHCIYTLVLMSVDRFLAVVYPITSRTIRTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGRKRVTRLVVVVVVAFASLWFPIQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9X1</accession>
    <name>T1G9X1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02154088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9X1"/>
    <dbReference type="STRING" id="36166.T1G9X1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000012-RA">
        <property type="protein sequence ID" value="MESCA000012-PA"/>
        <property type="gene ID" value="MESCA000012"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220900_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF43">
        <property type="entry name" value="PAIRED BOX POX-NEURO PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="135"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="24935" checksum="5A8A45515F02CEF9" modified="2013-10-16" version="1">MFAWEIRDQLIKQRICDPNSLPSVSSVNRILRNGGLMTDEMTTNEQNVIMQESYLKQQLFAVSPASVPSGGQPDKELLKKPEKRQKLSSSSSSSPVSPAAVAATAASVTIVPLALPIIKEEISCDETSSMLTAKTTTGGTKHINENNNILDDSSLSSSSTTSSTSSTDDVNEETVENSRIRGYLRRAAKGFGFILIRAQSIKTYARVVNQTRLKKRRFEEQFEDLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA75</accession>
    <name>T1GA75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA75"/>
    <dbReference type="SMR" id="T1GA75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000123-RA">
        <property type="protein sequence ID" value="MESCA000123-PA"/>
        <property type="gene ID" value="MESCA000123"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062794_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000123-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="31001" checksum="B2B235F6404CA253" modified="2013-10-16" version="1">MANQKLSEILRISLNMDLKKTSLSILSWHVYIWKMTGLYPMKYTENWKKYCHYCFCIILHGSFTFYYAFSITFQSFLVTRIKDLLANIFISATFIAIFIKLIVIRKNSSYFTHILELSCEFEEKYKESKFAQEQQKIFKRKTRTYSISICTATLSLLYEAYFPFDPLEGDLRYGAIMFYQTIGWLYMGYINVFADSYPTILIYLLTNDMNILGDQISQIAKLNDMGKITRIFPYNNQFYKDCNKSGRNKYVSQKIFDSGDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAE6</accession>
    <name>T1GAE6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAE6"/>
    <dbReference type="STRING" id="36166.T1GAE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000201-RA">
        <property type="protein sequence ID" value="MESCA000201-PA"/>
        <property type="gene ID" value="MESCA000201"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081599_0_0_1"/>
    <dbReference type="OMA" id="LCECEAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="71"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000201-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="239" mass="27211" checksum="35A9B63F88D9893A" modified="2013-10-16" version="1">MYSPKKKLNAPGNFQFPFTPYKIQSDLMKELYQILENKEIGIFESPTGTGKSLTLTCSPITWFLDHKSLVNSELDSEIEKLAREIAKSETETGKSVDWITEQFADIQKKKSLVELKRIQAALNEHEEKIKELKESYNNLGGVPGLQATGISFQIEEELDEFDVLNDFEEDSASKTEEIDDEMKPKMVKLFFASRTHSQLSQVINEIKKSPFGKDIRCTTLASRQNLCINPNVSVHGWTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAI8</accession>
    <name>T1GAI8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000245-RA">
        <property type="protein sequence ID" value="MESCA000245-PA"/>
        <property type="gene ID" value="MESCA000245"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1596382_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="19161" checksum="6B6BB54B744A5590" modified="2013-10-16" version="1">MDVTNSVLYSCNCVIGKKWGLQTSMIHWIFCIMVLPILSYGALVCWTATRRKSVIKKTNISAKNCLSKHHRFVRSTPTAALETILNLIQMDLKIKDIAGRKTASLYSWHLHPYAICGFCSSMCRKWVLLINEFFDEAKEIICKPCHMCLHVGYVVQWSPCRCVLYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB02</accession>
    <name>T1GB02_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U6 snRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.346</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. METTL16/RlmF family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.346" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02394218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB02"/>
    <dbReference type="STRING" id="36166.T1GB02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000421-RA">
        <property type="protein sequence ID" value="MESCA000421-PA"/>
        <property type="gene ID" value="MESCA000421"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027534_3_0_1"/>
    <dbReference type="OMA" id="HQGRYDF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120048">
        <property type="term" value="F:U6 snRNA (adenine-(43)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017182">
        <property type="entry name" value="METTL16/PsiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037350">
        <property type="entry name" value="Mtase_ZK1128_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037350-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000421-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="297" mass="34612" checksum="530D961E5FFEA7AB" modified="2013-10-16" version="1">MTQNVFMHPRNDFKIPPNYTDLAIKHADFRKVCILNLEGTVRLDMRSPKTLQVLSKTLLREFFELEVDFAPGSLIPTIPSRLNYLLWIEDLSKSLNWKEISGIDIGCGSSCIYSLLAAKKFKWNMLALEIDQGNFSFAKRNVENNMLNENIEVFLQSDKNVIFKEYFEKKPSKKFKFCMCNPPYFENIGRLPMNRKRRREAKNEPTGNLNELAFQGGEVEFIKKIINESCEFKDHIEIFTSLIGVKKNITSLEEFLKSKDITNFVITSFCQGNTTRWGIAWTFNNYLKLNIRNDEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBE2</accession>
    <name>T1GBE2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBE2"/>
    <dbReference type="STRING" id="36166.T1GBE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000574-RA">
        <property type="protein sequence ID" value="MESCA000574-PA"/>
        <property type="gene ID" value="MESCA000574"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_548940_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000574-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="497" mass="55670" checksum="2F297330AB075D5E" modified="2013-10-16" version="1">MLINKLPYGRNITTTTKKYDNDEDTVSMGEAVPVVQEDNDMIDMVTKTTTDNGLSTWILLNNSTLKTTTARPENKEKPTIIRKKNTKKPTPPPTSVSTTLAIRDTKPTTKKPPTTTTTTTTLRPTVESTTFLILEPKDADFDLPQDRSPGTTKKPKRKTTATGEKKPTKANPSKEKPLSTKIYNYLAREVMPTVGVGLVGLTVAAGLATYFLGPFGGLRRSYDNAADRKDDFYYNNDDEYGGSGQSEEEVFGKVIAGMPANSPYRNSMNNMKYNAYHRNNQPMQRPSNSGIRIVQAPPQHYRVRNSGYVPGHMPTYMNTKRNDDRTQIYRYNNEPSAMSYKKHSIQEVSLTTSAPQMVSETTTQSSYFPQYSSSTSENIEIVTPSPVKEEDMDYMNEKPTNHKYPSADIPDSTNDQQMDNNVLKKRTNKYVVGTVVDTNNEDNDLMGEFPEHGPRRRRQVTGNELDAVLPDEEPEFTTIASVDGEELKSTEPSTKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBF4</accession>
    <name>T1GBF4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02023721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBF4"/>
    <dbReference type="STRING" id="36166.T1GBF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000586-RA">
        <property type="protein sequence ID" value="MESCA000586-PA"/>
        <property type="gene ID" value="MESCA000586"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_5_1"/>
    <dbReference type="OMA" id="NNYDFIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="49"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000586-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="21488" checksum="17D092D6903C1CD2" modified="2013-10-16" version="1">MELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYVDSQIFCLHGGLSPSIDSLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHTNGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAALMELDDSLKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBS2</accession>
    <name>T1GBS2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBS2"/>
    <dbReference type="STRING" id="36166.T1GBS2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000710-RA">
        <property type="protein sequence ID" value="MESCA000710-PA"/>
        <property type="gene ID" value="MESCA000710"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_960720_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577195" description="Chitin-binding type-2 domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="103"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="32981" checksum="96B0AABACD6DFD30" modified="2013-10-16" version="1">MKIFNITFVAIFLLILVVVSKTKADEDLNSQIVSNIEHSGDSEDATTLEPEGEGIEEITTLEIEIEQQEENNNEIQSATESPDVQIQTTLGIIDDDDGKDVETIVCEKEGNIAHKEDCHSYYTCSLDPVDGILKPTKNSCRENQAFSVNFDICTRDISSCNIGPECLTKGKFPDPSSENSYFWCVENYNKDAFRKFKVQYLSAPFVPPTNTITDEDLVRRENKLLNGIEKKLAKDEKRRKKEEEKLQKDEEKRKRKEEKEAQKAAAKAQKEQDKKNKGNKKDESEGCIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCC0</accession>
    <name>T1GCC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCC0"/>
    <dbReference type="STRING" id="36166.T1GCC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000930-RA">
        <property type="protein sequence ID" value="MESCA000930-PA"/>
        <property type="gene ID" value="MESCA000930"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167263_0_0_1"/>
    <dbReference type="OMA" id="FRVFICL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005542">
        <property type="entry name" value="PBX_PBC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03792">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51978">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBC" evidence="3">
        <location>
            <begin position="36"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000930-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9706" checksum="D790D728A65008FC" modified="2013-10-16" version="1">MEDPNRMMAHTGGMMAPQGYGMPGQEDGSVNENEVRKQKDISEILQQIMSISEQSLDEAQARKHTLNCHRMKPALFSVLCEIKEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCN2</accession>
    <name>T1GCN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCN2"/>
    <dbReference type="STRING" id="36166.T1GCN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001052-RA">
        <property type="protein sequence ID" value="MESCA001052-PA"/>
        <property type="gene ID" value="MESCA001052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1181061_0_0_1"/>
    <dbReference type="OMA" id="HAPKQMV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="3">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="22176" checksum="1D6513B687142B6E" modified="2013-10-16" version="1">MAKRIQMRKQRFMTIQKMAEQKKLQAEGGGQPPPNQTNPNTGGDHGHGHGHGHSHGHPHAPKQMVGNRPMGFSNMQQNVGQRGCSLVGPLFQEVRFKVHDPKAHSKGGTLENTVNGGRGGMGPPPAPAPAPEGGGGGDGGEAGAIPASILKPGKDINKLLGITPSDIDKYSRIVFPVCFVCFNLMYWIIYLHVSDVVADDLVLLGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDC6</accession>
    <name>T1GDC6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001310-RA">
        <property type="protein sequence ID" value="MESCA001310-PA"/>
        <property type="gene ID" value="MESCA001310"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852277_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001310-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7202" checksum="774691AB7E056620" modified="2013-10-16" version="1">MDDVISRDARVIGVPNWRTTRLCNGLDMCGPGYGRSGRAIFFINLKTRLVMGGVDMLILNLKEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDG0</accession>
    <name>T1GDG0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02039725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDG0"/>
    <dbReference type="STRING" id="36166.T1GDG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001346-RA">
        <property type="protein sequence ID" value="MESCA001346-PA"/>
        <property type="gene ID" value="MESCA001346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_788216_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000679">
        <property type="entry name" value="Homeobox protein aristaless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000003">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000069">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF41">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0563">Paired box</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Paired" evidence="14">
        <location>
            <begin position="148"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="39"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="276"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="39362" checksum="E6903C838F1750BD" modified="2013-10-16" version="1">MNIFCKCYSFLELPSEDDTTRDGLESIEDQARLRLKRKLQRNRTSFTNEQIDNLEKEFERSHYPDVFARERLSAKIGIPEARIQVWFSNRRAKWRREEKIRTNGDRATPATPATSTTPNISCVTPPLPSEGTPTYCGTPTTSSTIWESYGGVNQLGGVFVGGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVAKAEVVEKISQYKKECPSIFAWEIRDRLLSDGICNSENIPSVSSINRVLRNLAAQKEQQQQQQQQQMSTSSSSPGCQSQNPIPPLSPAVKPSENSDKSDLFYEKFRMLNSGSDANQLVGTIKVPGTREFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDU7</accession>
    <name>T1GDU7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02017016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001498-RA">
        <property type="protein sequence ID" value="MESCA001498-PA"/>
        <property type="gene ID" value="MESCA001498"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560892_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9126" checksum="1DD5D9A7A47EDA18" modified="2013-10-16" version="1">MRLDILPLLFTSFDSNTIQVQGAALVATANVHEIIDEASLRVTVLPKIKAVYEKNALDAKIVGNVMTCIERILDKLDKQQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE10</accession>
    <name>T1GE10_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001567-RA">
        <property type="protein sequence ID" value="MESCA001567-PA"/>
        <property type="gene ID" value="MESCA001567"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1361814_0_0_1"/>
    <dbReference type="OMA" id="MNIIQLA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001567-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22732" checksum="DE0C5F9FF945811E" modified="2013-10-16" version="1">MEAYKNLYSDPKSPLSPIPPIPDSFNCNLLKTTPKTVIAKKPVVRTLAQRAQNQAANKARDIPRKPLIRIENPHGESVYNNPNRPCLQPSNSKLHQKRVEAKLGAGAGQHRCHCQCKQKLSQQKVEQKQYRAPSPVAPRALSPRIDNILKEAGLHKYAGLLKKEEIDYFVFKMLLEKDLTELGIPKNDIPSFMETIRKVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEK3</accession>
    <name>T1GEK3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02022240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GEK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001768-RA">
        <property type="protein sequence ID" value="MESCA001768-PA"/>
        <property type="gene ID" value="MESCA001768"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001768-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="38068" checksum="C2A0C2B49A263A7C" modified="2013-10-16" version="1">MEISLFFLFLIIFGLILYIIYKIQTYWKSRGVLHLKPKFPFGNISIKEPNFKSFRKVYNKFKGSALFCGFYFFLKPAALILDLDLVKNILIKDFSNFTDRNLFSNEKDDPMSGHLFLLGEEKWRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKQTPQHIIDFFMSVVKETVEFRETNKVQKHDFMNLLIDLKNNQETTLSIEEVAAQCFVFFAAGFETSSSTMTYCLYELASNQDVQDILRKEILLILEKFNGEFSYECMKEMRYLDQTIKETLRKYSTLPSLIRNTKETYNVNHDLKIEKGTMCIIPIDAIMNDPEIYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEP1</accession>
    <name>T1GEP1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynein heavy chain AAA module D4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEP1"/>
    <dbReference type="STRING" id="36166.T1GEP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001811-RA">
        <property type="protein sequence ID" value="MESCA001811-PA"/>
        <property type="gene ID" value="MESCA001811"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1505161_0_0_1"/>
    <dbReference type="OMA" id="NACKVYL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF71">
        <property type="entry name" value="DYNEIN, AXONEMAL, HEAVY CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="2">
        <location>
            <begin position="79"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001811-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="20759" checksum="8771C33591BFAF18" modified="2013-10-16" version="1">MVRNACKVYLRFSAEQAMADRLEPGEKLTDNDLRNLFFGNYMEPDAEPKYYDEVENYEKLEKVMLYYLKDYNEFSSAPMDLVLFRFAVDHISRVSRVLQMPRGNILMVGMGGSGRRSAAKLAASIAETKLMTVEITKTYSMNEWREDIRKILMTAGMNLNHTVFLFSDSQGKQQKHQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GER1</accession>
    <name>T1GER1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02098182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GER1"/>
    <dbReference type="STRING" id="36166.T1GER1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001832-RA">
        <property type="protein sequence ID" value="MESCA001832-PA"/>
        <property type="gene ID" value="MESCA001832"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142469_0_0_1"/>
    <dbReference type="OMA" id="NEFNTIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090364">
        <property type="term" value="P:regulation of proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011943">
        <property type="entry name" value="HAD-SF_hydro_IIID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051658">
        <property type="entry name" value="UBLCP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02245">
        <property type="entry name" value="HAD_IIID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="4">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001832-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="18625" checksum="E833236D70AA5EAB" modified="2013-10-16" version="1">MRPYLHEFLTSAYEDYEIVIWSATGMKWIEEKMRLLGVSTNSAYKVWFYLDSSAMISVYTSNKGVIDVKPLALIWGKYPQYSSKNTIMFDDIKRNFLMNPRSGLVIRPFRQAHLNRDTDKELVKLAKYLKEIARSCDDFNKLSHRKWERYLRKHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GET1</accession>
    <name>T1GET1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GATOR2 complex protein WDR24</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02070568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GET1"/>
    <dbReference type="STRING" id="36166.T1GET1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001852-RA">
        <property type="protein sequence ID" value="MESCA001852-PA"/>
        <property type="gene ID" value="MESCA001852"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010233_0_0_1"/>
    <dbReference type="OMA" id="EPMWLIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16693">
        <property type="entry name" value="mRING-H2-C3H3C2_WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001852-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="743" mass="83692" checksum="1A99E0B3EC918583" modified="2013-10-16" version="1">MTAESSTVSLRICQEGHANALSISKDSSQIAVAGRMLKVYSIEHDGFIESCNLRAGKSQNLSYSSNDVCWCPTDTNILATAATNGVISIWDLSKFGRQKQLLTYTEHERTAHTVTSIPQNKHKLLSYCQSSNSVFTIYSNSEGVRDIKFSPLTTNVFASVSENGTVQMYDIRKYEKCLLQFTAHSGPIYCCDWHPIHNWLATGSRDKQIKIWNMENKAALEYTIPTIAVVGRVKWRPEKNYQIASCALVTDYSVHVWDIRRPYMPYASFNEHTNITTGIAWKGNNPQILLSTSKDSTIFKHAFKDASKPSSRANPQGTSFGPTGDLFFSMKCKAQPPQITPRGNKVTAMLARQKSITSNEQFHLAQSASFRLLLKPSTTGYEFDLKSGKYKESDGFIGCAKEYILRAKSLAEVCEHNAAVSKKYGKHTAATLWNFVKKPPQRSSFSNQKNLNRRVIQSKPSTSLNKSESKIDLNESKELDSRRGSKDFDSISRIYYSRVSNQPVILENSEGPFEDYRSLRDGFLYVGPHDVTTALSFPGTNLHHDIQAARNHEDIRNDHDETMTPDVMSVLKVPIVPQVPIWEPHHIISECLHLQADIGDVQTPLCIYISLGQSRKELQIDEQVVESWFHSYIDLLQGYKLWNQTSEIIKISWFQSVKELNQQSTSVHTSCGKCNRQILGTVGWLCSNCKTTDCSKCSICSLIVKGLYVWCQGCGHGGHLQHLKEYFSINSRCPKCGHDCEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GET9</accession>
    <name>T1GET9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ERAP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GET9"/>
    <dbReference type="STRING" id="36166.T1GET9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001860-RA">
        <property type="protein sequence ID" value="MESCA001860-PA"/>
        <property type="gene ID" value="MESCA001860"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006744_0_0_1"/>
    <dbReference type="OMA" id="WEYLEAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF290">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="3">
        <location>
            <begin position="132"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001860-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="32580" checksum="E2A5949CB23A314E" modified="2013-10-16" version="1">MYDYYKGCSMFRMIENVLTKNTMDKAMRNLIQKYQYSNFDEDDLLAEFQSAATEDNIQLFEHQGSIDRVFKSWFHKPNYPIKNETHLWDIPLNFATSKEPNFEETTAEYILPKVPILTIRANGKFMLDQNEWIIMNKQQTGLYVVNYDQHNWEVIAEALAYSHESIHYLNRAQIVRDVMVLHANNYLSLEIAFDVLSYLSQETDLTVWRSAINTLMYFNTVLEDGKAEEKFKEFVREIIKEIVEIVKIQDSDPEEKAFISDTRISLLELACEVDHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEU0</accession>
    <name>T1GEU0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02162220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001861-RA">
        <property type="protein sequence ID" value="MESCA001861-PA"/>
        <property type="gene ID" value="MESCA001861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485924_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10458" checksum="C1D415EB047AF5F2" modified="2013-10-16" version="1">MSFWIWKRPILKAQQLKRLSEHKYSCASASLLDPNYKNGGTGYFSFKERGLRLRPRPDLRYHSLLLYFTPEIKVGQRHRHTFSFHFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEX5</accession>
    <name>T1GEX5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001898-RA">
        <property type="protein sequence ID" value="MESCA001898-PA"/>
        <property type="gene ID" value="MESCA001898"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_608701_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080:SF144">
        <property type="entry name" value="SPINDLE AND KINETOCHORE ASSOCIATED COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080">
        <property type="entry name" value="THAP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001898-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="395" mass="44940" checksum="154B09D066A1B27F" modified="2013-10-16" version="1">MVKNWIEELMFPGHDLKTFKMFWKFRIANTLAIISPILLNKPLKALVPTLTAGPSGAGISSKQHIVKSPNSTIRIRTAGTTIKPTQAAAQIFNNGLTTCNTSNSTHISRQAVFLKTQVQKPKSIRNLTIENIEKSPQLYIGLSKERLNVLKNVTSRLTDITTMDLYVTLKKIRLNEDFNILSDCFELEDGEVQKVFSTTVVKLAKYMKSLIRWPDYKKYYDRHKNLPIAFRSRLSYVQSLVECIDTDFQPKTLHPHDIQLDSMYCDKLKFLLAMSPNGVISYISDMFATNQLSNDLSVFKASNFDRNIPAYLSLVAEPGKAIRQISCKHNSQIHLDEENADAKTEIVSQDPSSNTIFVRMGNFQTRQEILQCPLFNCQVIDNCRCNQFAKIDDFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF26</accession>
    <name>T1GF26_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02140184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF26"/>
    <dbReference type="STRING" id="36166.T1GF26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001955-RA">
        <property type="protein sequence ID" value="MESCA001955-PA"/>
        <property type="gene ID" value="MESCA001955"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_0_1_1"/>
    <dbReference type="OMA" id="NERSAMW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF349">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="197"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001955-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="449" mass="49771" checksum="86848BC25C7C8371" modified="2013-10-16" version="1">MVKVKKSFTFSFAKKRKTRGGIRDERYFESFNDNKKMTERQPLMEQDELERGRPRPDYRSSPDNGLVDVNSDDSASSYDEKFKTNEDYDPAQHRSLDHPTSNMDTLIHLLKGNIGTGILAMPAAFKNAGLYVGLFGTLLMGVICTHCMHMLVRCSHELCRRMSVPSLNFAQVAYSSFETGPIGLRRYSLLAKKSVDTFLFITQIVVGLAITFMYLLHDLPRTSTVKPVASWATLPLYFGTAIYAFEGIGVVLPLENNMRTPQDFGGATGVLNTGMVIVACLYTAVGFFGYLKYGDDVKGSITLNLPPGDVLSQSVRIMMALAIFLSYTLQFYVPVTILEPFVQRHFDNERSAMWSGIGLRVGMVIFTFVLAACIPRLESIISLVGAVSSSALALIAPPIIEIVTFWPVGFGKYNGMLWKDIGILIFGLLGFVFGTYTSVADILNPHSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFP2</accession>
    <name>T1GFP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02079403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFP2"/>
    <dbReference type="STRING" id="36166.T1GFP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002189-RA">
        <property type="protein sequence ID" value="MESCA002189-PA"/>
        <property type="gene ID" value="MESCA002189"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018310_2_0_1"/>
    <dbReference type="OMA" id="WAMDRAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000017">
        <property type="entry name" value="cAMP-dependent protein kinase type II regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="108"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="255"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002189-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="36571" checksum="499BAA63F9AF85C9" modified="2013-10-16" version="1">MSAQQNKQRLQVPEARRRLCFEFFTKLQESRKAAAALKQNEDLPSPDESVLSQDEEPVVQRFVGRRKSVFAEAYDPENDDDDEGATAIFPKTDEQRARLTESVKNILLFRSLDKEQMNQVLDAMFEKKVVADEYIIRQGDDGDNFYVIESGVYKAFVNEKHIHTYNNSGSFGELALLYNMPRAASIQAESEGFLWAMDRQTFRRILLKSAFKKRKMYEKLLDSVPMLKALQLYIFTNYKDLNFNSFLYKIRVHNFILTGEVADGMYFIEDGKISIQISQDGGEVEISQLGTGQYFGELALVTHRPRAASAYALENCKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFR5</accession>
    <name>T1GFR5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA002212-RA">
        <property type="protein sequence ID" value="MESCA002212-PA"/>
        <property type="gene ID" value="MESCA002212"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471638_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002212-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10556" checksum="40B1A338C523567B" modified="2013-10-16" version="1">MHEEFLDEYFLNDFNMWDDMNFCIDFDFSKLNSHESSGSKRVSRYNCPVCEEGFDHQKDLYIHSAEIHXASKCIFVHYVKVSLQLWII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGB8</accession>
    <name>T1GGB8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABCA1-4-like C-terminal R2 regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGB8"/>
    <dbReference type="STRING" id="36166.T1GGB8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002432-RA">
        <property type="protein sequence ID" value="MESCA002432-PA"/>
        <property type="gene ID" value="MESCA002432"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608789_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABCA1-4-like C-terminal R2 regulatory" evidence="1">
        <location>
            <begin position="10"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002432-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9451" checksum="F542BB7921A9763F" modified="2013-10-16" version="1">MICVGQSATYLSAQIERYLIYHIPTSLRQVKWSYIFGLMEDIKEDYFIEDYTISQTTLEEVFFALAGNQILRKRRRFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGI4</accession>
    <name>T1GGI4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035237">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGI4"/>
    <dbReference type="STRING" id="36166.T1GGI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002504-RA">
        <property type="protein sequence ID" value="MESCA002504-PA"/>
        <property type="gene ID" value="MESCA002504"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1210994_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="154"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002504-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="229" mass="25583" checksum="4C9BE10025FEEF7B" modified="2013-10-16" version="1">MLLRKSLEKRGNNVNPSGRLAEFLSEYAGLLAAQGALDTALEYIGPTDDSQLEDLRERLYYALGHKSQQQATNKPIIFTSPPYDQIMPPYGQQPVVQQPSPMQFNPSNFNTNALNVPTSAPYQQPMMGQPQAQPFMTTVLWNNYMPGVPPIEITPLTPPPQQMKRNPTPPPGWNDPPALMSTRNQKKNETLPPPTSATPITHPLFGVDPNQNQNGYYDPNQQYQKQCPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGL0</accession>
    <name>T1GGL0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02098123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGL0"/>
    <dbReference type="STRING" id="36166.T1GGL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002532-RA">
        <property type="protein sequence ID" value="MESCA002532-PA"/>
        <property type="gene ID" value="MESCA002532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052285_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13409" checksum="A8472A658AB284CC" modified="2013-10-16" version="1">MAIKADKQLALNFNEGVLLQTIEKVQAATAGNNCTKIQVFPFIALENGGGAFVIPYIIALLIALCQFSRKGSVQIYERHWIWTNIYHNCSDNVLCHNYGFGFNILCSFFQLSTSMDLLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHG5</accession>
    <name>T1GHG5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02083753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHG5"/>
    <dbReference type="STRING" id="36166.T1GHG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002864-RA">
        <property type="protein sequence ID" value="MESCA002864-PA"/>
        <property type="gene ID" value="MESCA002864"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186939_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="64"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002864-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12758" checksum="59FFCFC89AFAF561" modified="2013-10-16" version="1">MTSTEATYRIEERKPANGVIVPMVDVYVSQENIPFNRDWSNRKCTMTAFFPSHIERTLNFKGRPDDVWVVTVPKCGTTWMQETAWLIYNDFDFETAKNTPLMDRSPFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL3</accession>
    <name>T1GHL3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL3"/>
    <dbReference type="STRING" id="36166.T1GHL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002920-RA">
        <property type="protein sequence ID" value="MESCA002920-PA"/>
        <property type="gene ID" value="MESCA002920"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_483396_0_0_1"/>
    <dbReference type="OMA" id="NARICEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="249"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="522"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002920-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="564" mass="65886" checksum="0324335D3A617AEF" modified="2013-10-16" version="1">MKESHSNAVQEALKKELDRAIEELTHCYDDALERLKRSRHIHFDQNRKPSEGRILLTEFADIIAHKALVDARISVLSGDRCQLWKVTNQPKNKFSVTSLQKYENMFDDMASDLNLSNPGDILAEADFEFLYKNNLIDFIKDKSSIIEITLSLNKLENSLIKLNNAIEPTSKKLNSLPAMRLETLQDVCLKCRQLRDHMENLCNSRQQKLNNNNARICENCHIIKTNLSALQVQHDLTVKKLQQDYTKDIMALRQQLDCNLKSMKNLENERNTLLEEISQQTNVVEGRERELSAITDKLKSKEQECHLKDKDLKRISQNLEEKSRYRDQKYLKQDEEYIVLLKERDYVQIELNKERDKTRKLEKRLEMLELEHTKQLECLQEAYREQILAHCSDFSNIEEEESFRQRYQSEIEQLRVSLKTKNRQKSSRTTEYHHSYHQLQQNQQQQSPRPKTINLLNERSSILSNIKGNGTSSNPTRTTNLAISLDSFTNLLNNKKNDDDVLLITKQIYNNAMLQQQQHSTNNIHHSQHNASSSSPSSLGGRDVEPKPHINATTSHLRRFELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIA7</accession>
    <name>T1GIA7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIA7"/>
    <dbReference type="STRING" id="36166.T1GIA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003177-RA">
        <property type="protein sequence ID" value="MESCA003177-PA"/>
        <property type="gene ID" value="MESCA003177"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="OMA" id="CADEDHR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003177-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11753" checksum="4D4158D7871A037E" modified="2013-10-16" version="1">MCIIPADSIMMDPNIFPDPEVFNPDRFTPEEIQNRHPMAWLPFGDGPRNCIGLRFGKMQAIIGLVALLTKYRISISHKTTVPLQFDPTSNLRAAKGGIFLKVEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIK4</accession>
    <name>T1GIK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02065464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIK4"/>
    <dbReference type="STRING" id="36166.T1GIK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003284-RA">
        <property type="protein sequence ID" value="MESCA003284-PA"/>
        <property type="gene ID" value="MESCA003284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1772753_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16979" checksum="2732BABFA0FBE98F" modified="2013-10-16" version="1">MIKSKGKNDCIETHSNSLILGLNLLKTVAVVFQNNCPHEWGKPFIHNKVFNRLLSCTSWILQQHSSYKLTIELLDVLINTGYDFLKDLLTFVGIHEQYLMDSLLLGKQSLEPNAMLLVKTTVHLLAKLVHFEKEWRLEHSKSLYTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ20</accession>
    <name>T1GJ20_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-Casp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ20"/>
    <dbReference type="STRING" id="36166.T1GJ20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003459-RA">
        <property type="protein sequence ID" value="MESCA003459-PA"/>
        <property type="gene ID" value="MESCA003459"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852242_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF37">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003459-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7115" checksum="0B088CA5AA9C2F0F" modified="2013-10-16" version="1">MVVFATPGMLHAGLSLQIFKKWAPNENNMVIMPGYCVQGTVGHKILGGAKKVEFENKQQVEDGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ22</accession>
    <name>T1GJ22_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ22"/>
    <dbReference type="STRING" id="36166.T1GJ22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003461-RA">
        <property type="protein sequence ID" value="MESCA003461-PA"/>
        <property type="gene ID" value="MESCA003461"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533510_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF40">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003461-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="511" mass="57968" checksum="1993830AED3FFAE6" modified="2013-10-16" version="1">MTLLSFVLPMIFDALGLFENWHPRQQLRLQLARIMILNLLNLYSLMFSFIYKISSSDENLQNLKVVSDNASIQEEKIWEALRRINDSITTTTFTPITTTNLFQEETQAASDFWTTTDLSSTLDMSSVSDFMSSSENWFSTSTTEEDYSSASSTTEDSSFFESTTWSSTTSILNTTYCYTIIHHCLSNHTHRHLDRESTFFSSTTMFPSESGTGSDSTTGLDESTTFLPDFSATIDASTESTFNSDATEPTFSTNEFTTQTTETTDETTKDYGEYKRFDFNESYFEYYDYFDNLDPIKDNDDFMVDTTDDSDNSIEIESLSHDRKKRDLPLTTVTTLKSLVDTTMETVAEKSAGDDSSSKMKESMAKSVKVFVANELDWGPDVSDIYLTEEEYKENYGDDLEEREGNCTQVICVSLPPPSVKDDTSTTPFELSTTGITDDSTEFTTEDFGISSSTEEITTKLSEYERISGELRKVQETKKYVSIQLTSMCWETSLGQELMKIVVFDTKDLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ43</accession>
    <name>T1GJ43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ43"/>
    <dbReference type="STRING" id="36166.T1GJ43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003484-RA">
        <property type="protein sequence ID" value="MESCA003484-PA"/>
        <property type="gene ID" value="MESCA003484"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818001_0_0_1"/>
    <dbReference type="OMA" id="IMTMAMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="10"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003484-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="15328" checksum="DFF6034E342C9571" modified="2013-10-16" version="1">MINTNLRVTVSLGLLAGAKVINVSVPFLFKGAVDNLNILNFDDPVNTTLALSTSLLIGYGIARAGAAGMNELRNAVFARVASHSIRKIAKNVFLHLHNLDLSFHLNKQTGALSKTIDRGSRGINFILSAMVFNIVPTISNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJV6</accession>
    <name>T1GJV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uridine 5'-monophosphate synthase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
            <ecNumber evidence="6">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the OMP decarboxylase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EC" id="4.1.1.23" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02123440">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJV6"/>
    <dbReference type="STRING" id="36166.T1GJV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003761-RA">
        <property type="protein sequence ID" value="MESCA003761-PA"/>
        <property type="gene ID" value="MESCA003761"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049275_1_0_1"/>
    <dbReference type="OMA" id="SAKHVCG"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000245">
        <property type="entry name" value="Bifunctional UMP-synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000025">
        <property type="entry name" value="Uridine monophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="16">
        <location>
            <begin position="257"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="14">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="14">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="14">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003761-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="490" mass="54383" checksum="E951C3089DC38BFE" modified="2013-10-16" version="1">MLDKQSTEMKNLVVKLYEINAFKFGDFKMKVGINSPVYFDLRVIVSYPEVMETIGNFVVEYMKDLNLEYQHLCGVPYTALPIATLAAVKVNKSMLVRRKEVKTYGTKKAIEGKYSVGDNCLIIEDIITSGSSILETVDSLRNEGITVTDAIVIVDREQGGKANIAERGVRVHTLFTLSFMLNVLKEAGHIQEETVQSVNKYIQAVQIKSDGSFANGNNVQVVNKLDRATMLFEERASMAKSKKAKQLFNLMAQKKTNLCLACDTTDPQEILDLAEKCGPYICLFKTHMDIVDSIPSNFTSDLSAIAKRHNFLIMEDRKFSDIGNTVALQLSKGPFKISKWADFVTAHSLPGQGVLKGIKAGLAEENPKDLNVFLLAEMSCQGCLIDEKYKENTIKMATESTDIDVITGVVCQNKECFAFPGLIQLTPGVKINENKDDLGQQYNTPEYVVKSKGADIGVVGRGILTAKSPEKAAVEYRDALWSAYLERTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKE0</accession>
    <name>T1GKE0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKE0"/>
    <dbReference type="STRING" id="36166.T1GKE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003963-RA">
        <property type="protein sequence ID" value="MESCA003963-PA"/>
        <property type="gene ID" value="MESCA003963"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186950_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF42">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003963-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12861" checksum="799CFE576F72C7EC" modified="2013-10-16" version="1">MKFSLKIIFNNLVTIVAIKIIDKTCLNEEYLAKTYREISILKVLRHPHITRLYEVMESSTMIYLVTEYATNDSKDSFGRFLRDVRDSKDFIQIPYVRNPVVFPSQRTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKF2</accession>
    <name>T1GKF2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093032">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA003975-RA">
        <property type="protein sequence ID" value="MESCA003975-PA"/>
        <property type="gene ID" value="MESCA003975"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984104_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588375" evidence="1">
        <location>
            <begin position="22"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003975-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14022" checksum="39BF51ACC8201FC2" modified="2013-10-16" version="1">MKFAVTLLLLFTVLVASFAAAEEEGDAAVDSLAPKKTEKRGILGGYGYGHYGHLSPYYGGYYGHGYGHGHYPYSHSYHHGYFGNHYPYXXXXXXXXXXXXXXXXXXXXXXXXXXXSVDTFTKQLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM11</accession>
    <name>T1GM11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004579-RA">
        <property type="protein sequence ID" value="MESCA004579-PA"/>
        <property type="gene ID" value="MESCA004579"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199972_0_0_1"/>
    <dbReference type="OMA" id="CSRKFRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588399" evidence="2">
        <location>
            <begin position="18"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004579-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12216" checksum="24E8FF5170C26E9A" modified="2013-10-16" version="1">MKFLLVALFAVVAIVSADVSHLSSANLLDSATQGPNPQDIATPEPEYIDIDLPEPPRPKAPAPSPINQIMSFSSFRQEVPGHTFGPDGYRYRRPAPRVYYRRVYRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMB2</accession>
    <name>T1GMB2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004681-RA">
        <property type="protein sequence ID" value="MESCA004681-PA"/>
        <property type="gene ID" value="MESCA004681"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624810_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004681-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8920" checksum="B71B882D7EFDE506" modified="2013-10-16" version="1">MDMVDPGQDGTHIHFFMNEFRSFPLGWFILIVLFGFRLDGNRVFMGEALNDPATAKKIETCISEKLKMIATDLDIESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMC4</accession>
    <name>T1GMC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GMC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004694-RA">
        <property type="protein sequence ID" value="MESCA004694-PA"/>
        <property type="gene ID" value="MESCA004694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000345_0_0_1"/>
    <dbReference type="OMA" id="THERRPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10975">
        <property type="entry name" value="CE4_CDA_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052740">
        <property type="entry name" value="CE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985:SF12">
        <property type="entry name" value="CHITIN DEACETYLASE-LIKE 5, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="194"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1247"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1339"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1550"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1641"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1853"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2039"/>
            <end position="2061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2107"/>
            <end position="2170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="789"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="948"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="969"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1247"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1430"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1553"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1644"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1856"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2039"/>
            <end position="2057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2113"/>
            <end position="2126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2141"/>
            <end position="2170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="2472" mass="280171" checksum="DCC23C2B99AB8B38" modified="2013-10-16" version="1">MSNHPKIVRSRSRPTLKPASDIVSKTQQFVDIYRYPPTRPDPLYPQPTPDRTAAKCRKDVCLLPDCYCGGKDIPGDLAKNETPQIVLMTFDDAINDVNTEHYEDLFKRGRKNPNGCPIAATFYVSHEWTDYSEVQNLYADGHEMASHTVSVWRQVSLASFCVAEVTLAQTKRASRITSSRAIGVNSRTNSNGVDFNCPEEFGYYPHPSDCTQYYVCVFGGALLESCTGGLMYSHELQTCDWPRNVGCEVQDARSSPSTSYRDITQGRSLQQPQPQSPSHIRFSSTTSKPTIAQQYHRQPSQSIQIQNPVQTLPPPPELSRIAPNPVITSRGQPKPIIESQEDIAKLYADTHDTLPPIEEEESDRQQRVYRGQPSTLSQVQRDRVVLSISLTQMQFQTMEKTISFDNDFDDNINILRRRKRDLDMTESNFTVTDEGDMEENIRITRQLRPRARWQSNSGLNYRSVDSSPYQQQTYSFGVFNPYLSPPAVPQQKNYKTSPVKQQIKQKTKPQPTTYNFDSGFQPITFTSQTPHQSYVGQSPALPAIYQSIRDIQDKRRPDPDNFAYYHLLQDDNSAINDGSEGYKYVTQYKKPTPSTPSVFQAYPNQFEKVTPRPHYASFSTIGGFYNNHIASRPEVNGLDNNNQVVSQYSIGGTSTQQTANIQPVVHLLPKLEQPNDFRPITTSTTAKPRLPSSQITNFNFENFMQNLKDTDMHKMDPSAKKVLEFIQQQNNFTYHNLVGKVPHGRQPFRPIPVDVPTTPRIEKGYESFIKSIPNPIPHTTERPTIFDYDDEDQDDDDYDIKPPQDMPAYMPSSETMAPRPHMHSQSQATTPYYRPDVIITTRRPFIYSPNQQQQQPHIIQTPYIHLSNDFFQHPSTQTETTPIMTTTTGTTSTPEITTTRKIYTIRPNPNRNRGNMKWKITKTTNKPNYRKRPQDEQVEQELDDNNRVETSSQGPQIFVQQQPQPQQQQHHHNPPQQQHHLRSKPLTEQNQNINNNNYNLNRNYYNTTTIQQQQQQYHQQQQQQQVPSSTPISLKSHSSQSLQSHPQIPFYSSYDDDDVDLYRDVEYNQQLQPQQPQQPQRGQSTYRPKEQLVTTPLPISVPQPPRDSFNKPILKPREPQYSNNNNNPPTQQQAHSPFSYSTDYEDSLAPQLDDENEYNEPTRQPIREEVEIQPLHGKPIIVSSTFGPATNNIDDEEDYYTASRKPIFVATTPFPTTLRTTITTTTRKPVITTTTPKEVIITTTPTPIRRRPTNTTTPSTPTKRSSPEVGINQTTTPSSILLTLSANTGYTNLPVSDLNRKTLHDLSEPKIESFSFITKPKLQAESQSKVHAVLPAPNKISEQSDKPSFSTAQPFQKHVDQTTTVKSTTTTFHTHIQNSSIGTTTFKFSSGSGLSGFSFKAHEKECIENIDEGKQNIKDNYRPRNLSNRNRQEGTPRPNELHPKPVKSFPVDSSKILKEKKIYPEDEISFKVINFKQPNPNRTQIILLPVTTPEQAKEILNSPPIVNERKFHKISEDITTTTQKRPIEIITYRPHKIRQQQLDVTTFRKESNSAENFDSATSRSLEQSPESETPRIIVTYQPRGFRQPIPTGASELSTPTRRNSLLLKEVYEPTTYTPSRSWLLPKIETISTTASSLSTMPINTKSLTSSTSPSTTTTTTTTSTTKAPTTTTSTTPYTTTTTSTTTVPPPTTSQLIRTTTLTPSTTNMFPTTTLSPKITFSELYNTPPYVPYRNENSDFINPPDLMSTTDRLQSLTNRALSFKDALNRNFETTRSPLKYVSPYKSLETILEEKSDKFKYNSYKTTIKPKFLNPYTSFGSQKPLRNYFLITTAPKINSSHLHLKSTTPIWRYMPSKTTTTSTEPTTTIASTTTTTATTIRTTTLPPETTTTPQTTTTSIAPTTSLTTEIVPEVRIGRGRGRYIGQSNTLDEDSPSLFPRNRFAFSTSTESSYRRKMLRSRPTPTKFYITRPTKSRSSQGRYEFIPAKLVSGSKPESGSEVILIKKLQKPLFSNTQENESFEKPRSEALVRESFENSIFTSISSSSSTTQSTSSTASSTTKEEIEKIVEITDRPVQYYAKLRQQTDDNIVPKQYSSKFRATVEMPEITLPIFPPSSSSSISSTSVEYESSTEEDDDFLPILNTTSSSSSSSSTTLSPTTSSTRSPTFPTRASRVNNAIKSSIAAASGRVNPNGCPISATFYVSHEWTDYSQVQNLYSDGHEMASHTVSHSFGEQFSQKKWTKEVSGQREILSAYGGVKLSDIRGMRAPFLSIGGNKMFKMLYDSNFTYDSSMXIRGMRAPFLSIGGNKMFKMLYDSNFTYDSSMPVYENRPPSWPYTLDYKIFHDCMIPPCPTKSYPGVWEVPMVMWQDLNGGRCSMGDACSNPGDSDGVFKMIMKNFERHYTTNRAPFGLFYHAAWFTQPHHKAGFIKFLDAINSMNDVWIVTNSQALQWVRDPTPLSRINAFQPFQCDFSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GME5</accession>
    <name>T1GME5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174646">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GME5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004719-RA">
        <property type="protein sequence ID" value="MESCA004719-PA"/>
        <property type="gene ID" value="MESCA004719"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490298_0_0_1"/>
    <dbReference type="OMA" id="RHRINKA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038875">
        <property type="entry name" value="PLA2_conodipine-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37687">
        <property type="entry name" value="AGAP006772-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37687:SF1">
        <property type="entry name" value="AGAP006772-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004719-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="556" mass="64986" checksum="292FC4BC89086628" modified="2013-10-16" version="1">MSVFRERDVQPYTQTELADEFLREIEEEREMEREEKYEKYKDALRNLWEKYQDQENEIITEPEEKRPQFMQKKRTYPVLPWLPYTDKRKRFPVAKRSPKVDKQSKDLEEIFGKPSDDEDKKKKRSVDSQGTGSATSSTTEVMPADGNHNHTEHGHGHNHTEHKHHKHEDEEESSESEEQDHEHDEYEEDSEEEYDPFSSNEEDDENRKKKRDTSKLKICQGHLCKKNNKGLGKSRQKKSIDWSQYFGLNRKKKSLSAPEDEELSPEKIEKMDRKLQFLEDEVLAETVKITGAYPEAQSLAEIRRMKENVMSNLATAYTLEKMRQALNNRRQSIENENHLGRNQIESFEEKAKRLSVKKEKVDFDKHTHDIDIEQDKMKEHVHGGNNMGGVNDSDDAEEEEKKRKKRKRTRYEKYPILPNDIGNFPDELEGLNYEPLNDAYLGNKNFYQNSYGECAIAENIDEKCKNIAVLSGAVNSELLTLCNVHQLCHLCSTTQVGCDFEYINEADRVCGRNANCLSSVRANMRMLRDSAGPQIGLKECMKIPCLNRLLKDINKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN47</accession>
    <name>T1GN47_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02038591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN47"/>
    <dbReference type="STRING" id="36166.T1GN47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004988-RA">
        <property type="protein sequence ID" value="MESCA004988-PA"/>
        <property type="gene ID" value="MESCA004988"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004988-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="25715" checksum="F440CD42B4FA70F0" modified="2013-10-16" version="1">MDNFLINTVKDILKVRKDNVDFMSIFLKMRDASLQKVSAQVYSFFEASLVTTSTTISTCLYELAMNDHVQQRLRLEILEFYNDVQGDLSYSNIKHCKYLDQVMFENLRKYPIANQLSRVCENNYRVEKWNLTIEKGVLVIIPISAIHMDPEIYPNPEIFNPDRFTSEEIDKRHSMSFSPFGDGGRSCIGKEFGNPKTDIPLKLDQTQITLPLGQIFLKVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNX2</accession>
    <name>T1GNX2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain coiled coil stalk domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNX2"/>
    <dbReference type="STRING" id="36166.T1GNX2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005285-RA">
        <property type="protein sequence ID" value="MESCA005285-PA"/>
        <property type="gene ID" value="MESCA005285"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1333282_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23977" checksum="B30B2E162A18E388" modified="2013-10-16" version="1">MSATNKFRQLFDDVSGRNPTVTSKFASSRLESVTNLNIWTMEQTVGLNENVSTFTGTMLAFITSIWENNWNFSSLKEKQIKYQEGVQSLPKIKERIQELSKKLEEISSEIRSLEQQKEPLRKKLENSIKEKDSTKQKNKEILNSMRNSLQNIKTTNNDIQRLTNDLAALEELNLSNAIQNLKESFKKVEKDLSEKLQAFRWELPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPG6</accession>
    <name>T1GPG6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mini-chromosome maintenance complex-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCMBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389530">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPG6"/>
    <dbReference type="STRING" id="36166.T1GPG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005496-RA">
        <property type="protein sequence ID" value="MESCA005496-PA"/>
        <property type="gene ID" value="MESCA005496"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_861347_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019140">
        <property type="entry name" value="MCM_complex-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489:SF0">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09739">
        <property type="entry name" value="MCM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005496-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="323" mass="36162" checksum="F88C67392C8DF464" modified="2013-10-16" version="1">MDFSFKIEDFVANEQLILENLKDFEKWKSIPMLDHTMLHNFKDNSLVRFRGMVQDMLDNETYLEKYQVKGEGNCRMQDGKFRDILVLGSGECVDHDSRENAFGERRSLFVISLPGINEWASSHEKGVIAAQTTPPQAESFGQKRSAEDTEMEQDEDVQQEPVQTAQNKRVCTSSVTANEAAAPKGLSAEYHLNSPISDRPSRACLVKVYSDLDSHTLNSVVDVIGFLSVDPAMDISTADVEMDDCDGILAANPPPSLIPRIHAIAVRKMKHLNPLLDTTLQTFEEHPLNFDVVYKDLLIVLTQCLFGDTLAAEYLLAHLISTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPJ5</accession>
    <name>T1GPJ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA005528"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of cell cycle process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="INSM1/2"/>
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        <property type="entry name" value="LD18634P"/>
        <property type="match status" value="1"/>
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        <source>
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        </source>
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        <source>
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</entry>
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    <accession>T1GPW0</accession>
    <name>T1GPW0_MEGSC</name>
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                <person name="Hughes D."/>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="MESCA005655"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="19595" checksum="0F83F8D39BDF82CD" modified="2013-10-16" version="1">MKKLDISIWKHVAQDKTTWKRLDGKIVSNTIDNEIISKAIDILFNIQGALMIFVRALLERRTYLLHGVSCQGLLIFVCSKLPCWRRDSTGMLPQPQLGNPSVQLKRNIILFLLTLSGVPKTEQILKSWSTSLLPGNNGRKVYNSAIIHPIAHKSIALLYRVECRRTSGALYLE</sequence>
</entry>
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    <accession>T1GQ13</accession>
    <name>T1GQ13_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
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        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA005711"/>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8098" checksum="8AABEBFC1206EB29" modified="2013-10-16" version="1">MTSLSSFGEKIALDFFLPHPVTTAVVPVFGVDVANSMELYSGWFLYATTSITCPPSLCKVDPTRTVNSWTLKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD6</accession>
    <name>T1GQD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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    </organism>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA005840"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
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        <location>
            <begin position="95"/>
            <end position="267"/>
        </location>
    </feature>
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        <location>
            <begin position="341"/>
            <end position="376"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
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        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
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        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
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        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005840-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="376" mass="40961" checksum="7A8C04B69E49A361" modified="2013-10-16" version="1">SAQENPNTANLSCQQPQNNESSASFYDFNENYKSVYENRNPLLIGSRNELNRGGFFPIQPLKSSLEKLNSQDQNNNTIAPQTTTASGAYITRSSRTATPSLGAGNNSDSSTSSPLKSTNCLKSPLKSPSNSNRLGSLSPQKHYHSQQRGNLPHSSSSSTVSSPSPTKLSNGNNIRRKSTSFEEGEHANGNNILPSESVQPQIQHVRTTKASRLRAAALDKKKDEIGLNSRHTLTSPSRKPQMDHSSSSSEPEGPSQQLKPTSSPRKLVPNISISMKQSLNELQFNGTVLLPSPKHEEPNKAAGDNNEEIVATVRPRSSTLNKNLLEDKSDIVNGILELELSPVSGRLPLSPDDRDKSSKRKSNLNRALNEECTENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQW2</accession>
    <name>T1GQW2_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Brachycera</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148282">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQW2"/>
    <dbReference type="STRING" id="36166.T1GQW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006027-RA">
        <property type="protein sequence ID" value="MESCA006027-PA"/>
        <property type="gene ID" value="MESCA006027"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266815_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006027-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11548" checksum="801E8CE6C5B9BC39" modified="2013-10-16" version="1">MFPGRKSNYSDRKQTNSDSSDQKGGTSSNVNVSSLSKKDASLIINSECCQNGPTPELDILMDYLFHPEERLDNPVSIEWVRWLIAGGRTPEEFASIELSAFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR14</accession>
    <name>T1GR14_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02395035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR14"/>
    <dbReference type="STRING" id="36166.T1GR14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006085-RA">
        <property type="protein sequence ID" value="MESCA006085-PA"/>
        <property type="gene ID" value="MESCA006085"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009654_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006085-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13331" checksum="E3CB182B133140AE" modified="2013-10-16" version="1">MYIFIAFGGIVGILGFSLAYYFYVKTKTKEMAIIQNHQPSADKNIYGSSQHNSLELTRLNGTTGSTTTTTTTTTAAPSFTGNGLISASGTPCHTLDRNPPAIRVPQYTLQDVEFVEELGEGAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRI2</accession>
    <name>T1GRI2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KAT8 regulatory NSL complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">NSL complex protein NSL2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-specific lethal 2 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Non-catalytic component of the NSL histone acetyltransferase complex, a multiprotein complex that mediates histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) at transcription start sites and promotes transcription initiation. Required for NSL complex stability and for transcription of intraciliary transport genes in both ciliated and non-ciliated cells by regulating histone H4 acetylation at 'Lys-5'- and 'Lys-12' (H4K5ac and H4K12ac). This is necessary for cilium assembly in ciliated cells and for organization of the microtubule cytoskeleton in non-ciliated cells. Required within the NSL complex to maintain nuclear architecture stability by promoting KAT8-mediated acetylation of lamin LMNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the NSL complex at least composed of KAT8/MOF, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02089189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRI2"/>
    <dbReference type="STRING" id="36166.T1GRI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006267-RA">
        <property type="protein sequence ID" value="MESCA006267-PA"/>
        <property type="gene ID" value="MESCA006267"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029808_2_0_1"/>
    <dbReference type="OMA" id="DKRESLC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026316">
        <property type="entry name" value="NSL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025927">
        <property type="entry name" value="Znf_KANL2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13453">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13453:SF1">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13891">
        <property type="entry name" value="zf-C3HC3H_KANSL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="KANL2-like probable zinc-finger" evidence="15">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="KANL2-like probable zinc-finger" evidence="15">
        <location>
            <begin position="311"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006267-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="387" mass="44393" checksum="FDD8123C9240E679" modified="2013-10-16" version="1">QTYACSVPRLEGYEYCIRHILQDNRAPFKQCAYLYASGKKCTQAAPKHDTKKDPHLTTFCFEHSRLLQLKKTRNSLGRYKEVETNEALLNDLSHYMNIDPPKTIPQNVSSELDEEIDVVSSNVQPFVSYEQIHGLEAINAAVKQKKRVLDYASDSSTDDELPTLNSASKAPECDVSDNESVDSQDEDLLKHAGIYTTEEAVLITKQKLDKLQGLYLDQIFRLQHLLREKRRKYLHALRKERETLCSIHSQPKNTPGERKMYEKLKAFNHYHKRHGMEAVLYKKYIEKRRKAYEASVNGNSSKSGGSHFPKCQFTEGGVKCAERVLPSCKYCRKHILEDKKQILFRACGVEKAGISCQDPIPSIFEDSTCVLHIKIAEPKAYTRKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRM9</accession>
    <name>T1GRM9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02168161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRM9"/>
    <dbReference type="STRING" id="36166.T1GRM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006315-RA">
        <property type="protein sequence ID" value="MESCA006315-PA"/>
        <property type="gene ID" value="MESCA006315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1612712_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18526" checksum="F5C0B6B898A0D87C" modified="2013-10-16" version="1">MYVIHKGVEVIVPIDAIHHNPDIYPEPEIFNPDRFLPEEIEKRHSMAYLPFGDGPRACIGLRFALLHAKIGMIALLNSLKFSPCQKTEKTIALEERNFFLTPKNGIHLQVELVQFLAVLDPPFSIDHAAPKISSSTILSYADSNLKWADVRVQLSKTYNSTGSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRQ7</accession>
    <name>T1GRQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">3-hydroxyacyl-CoA dehydrogenase type-2</fullName>
            <ecNumber evidence="3">1.1.1.53</ecNumber>
            <ecNumber evidence="4">1.1.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">3-hydroxyacyl-CoA dehydrogenase type II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Mitochondrial ribonuclease P protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Type II HADH</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(3S)-3-hydroxybutanoyl-CoA + NAD(+) = acetoacetyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30799"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57316"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:30800"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:30801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>11-dehydrocorticosterone + NAD(+) = pregn-4-ene-3,11,20,21-tetraone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42020"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78600"/>
            <dbReference type="ChEBI" id="CHEBI:78601"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:42021"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>17beta-estradiol + NAD(+) = estrone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:24613"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NAD(+) = 5alpha-androstan-3,17-dione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15994"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:41993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3beta,7beta-dihydroxy-5beta-cholan-24-oate + NAD(+) = 3beta-hydroxy-7-oxo-5beta-cholan-24-oate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42024"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78602"/>
            <dbReference type="ChEBI" id="CHEBI:78603"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:42025"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5alpha-androstane-3alpha,17beta-diol + NAD(+) = 17beta-hydroxy-5alpha-androstan-3-one + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42004"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:36713"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.53"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:42006"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5alpha-pregnan-20beta-ol-3-one + NAD(+) = 5alpha-pregnane-3,20-dione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42008"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28952"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78594"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:42009"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:22433"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:22434"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cortisol + NAD(+) = 11beta,17alpha-dihydroxypregn-4-ene-3,20,21-trione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42012"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17650"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78595"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:42013"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>cortisone + NAD(+) = 17alpha-hydroxypregn-4-en-3,11,20-trione-21-al + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42016"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16962"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78596"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:42017"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ursodeoxycholate + NAD(+) = 7-oxolithocholate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42028"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78604"/>
            <dbReference type="ChEBI" id="CHEBI:78605"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:42029"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 20">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.53" evidence="3"/>
    <dbReference type="EC" id="1.1.1.62" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02048390">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRQ7"/>
    <dbReference type="STRING" id="36166.T1GRQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006347-RA">
        <property type="protein sequence ID" value="MESCA006347-PA"/>
        <property type="gene ID" value="MESCA006347"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_42_0_1"/>
    <dbReference type="OMA" id="QDGQMGQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047044">
        <property type="term" value="F:androstan-3-alpha,17-beta-diol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008209">
        <property type="term" value="P:androgen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008210">
        <property type="term" value="P:estrogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000215">
        <property type="entry name" value="3-hydroxyacyl-CoA dehydrogenase type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658:SF8">
        <property type="entry name" value="17-BETA-HYDROXYSTEROID DEHYDROGENASE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006347-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="27200" checksum="8873028D892C17D5" modified="2013-10-16" version="1">MLSKTVVLVTGGSSGLGLGIVKHFSRNGAKVVLADLPISKGAEVAKELGDNVVFAPVDVTSEADVLAAIEIARNRFGRLDLTVNCAGISVYIKAFNFNTKVAHKLEDFTKVLMVNTVGTFNVIRLSAGLMGENEPNQDGQRGVIVNTGSIAAFDGQKGQAAYAASKAAVAGMTLPLARELGEQGIRVCTLAPGLFNTPMLQAVNDKDRSILAKSIPFPQRFGHPEEYAQFVQAIYENTIMNGEVIRIDGGLRMRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS26</accession>
    <name>T1GS26_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006474-RA">
        <property type="protein sequence ID" value="MESCA006474-PA"/>
        <property type="gene ID" value="MESCA006474"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624813_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006474-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8722" checksum="BBC3CE6B3F66E491" modified="2013-10-16" version="1">MYSHVWELVKRTQLLEEAFNGPMERQVLNSPSFARKNLIDATSSTQQAATATTTTGIPLSPRVSRRQIQNKASPGFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS70</accession>
    <name>T1GS70_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L37, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS70"/>
    <dbReference type="STRING" id="36166.T1GS70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006518-RA">
        <property type="protein sequence ID" value="MESCA006518-PA"/>
        <property type="gene ID" value="MESCA006518"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521540_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052482">
        <property type="entry name" value="mtLSU_mL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010793">
        <property type="entry name" value="Ribosomal_mL37/mL65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15889:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15889">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07147">
        <property type="entry name" value="PDCD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006518-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="20236" checksum="C8226BD882F0F88B" modified="2013-10-16" version="1">SKKPISKIGQVDGEIPELFPIHETISIPKKNIYLDEDLYPIKSSTYGNPHTIFIHFSKEDVQNLHETPVTPNQFKSRNMLKAFTVAASRARQLYGQVQDLPEPIVVQSIQTDGKSFHFGLFQLNTLNLEGLDGLKNYWFQLESMDLFNDCGVKHGKPTLEGYNKDVFRILNAFYNNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSR3</accession>
    <name>T1GSR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02169819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSR3"/>
    <dbReference type="STRING" id="36166.T1GSR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006724-RA">
        <property type="protein sequence ID" value="MESCA006724-PA"/>
        <property type="gene ID" value="MESCA006724"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048483_0_0_1"/>
    <dbReference type="OMA" id="DKMVNFF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF28">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006724-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="292" mass="34168" checksum="031B3D0962872461" modified="2013-10-16" version="1">MEHEDLRTKSWFLSNNPGPLFTILATYLYFCLYAGPRYMRDRKPFELKTTMIVYNIFQVVLSYVLFHEGYHGGWGFGKHQYSYTCQPVTYGTDSTSMRFALKKFILCRKLYHPENGQSSLVVYIAKITELLDTVFFVLRKKDRQISFLHMYHHTLMPVCAFIGVKYFAGGHGTLLGFINSFIHIVMYAYYLLAAMGPRIQKYLWWKKYITVLQIVQFLIIFVHTFQLQFQPSCNFPKSIAALLTFNAGLFTYMFSSFYVKNYKKEQKIAAKKQKMEEALDINGNSKAPIKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT03</accession>
    <name>T1GT03_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT03"/>
    <dbReference type="STRING" id="36166.T1GT03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006819-RA">
        <property type="protein sequence ID" value="MESCA006819-PA"/>
        <property type="gene ID" value="MESCA006819"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_807928_0_0_1"/>
    <dbReference type="OMA" id="HYPGHFD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006819-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="344" mass="39002" checksum="10C3AC49408CB86D" modified="2013-10-16" version="1">RAVDYQPYPFQQLISGSGKQQQPTRTQSQARRPASPGTRPVYPSYSPYMSSNGVNRNGIPSRIQDRFTPGEATAAQSFTKHRNPVYNPRPSASNTNANYVPPKNLYSFPPNDVNYPAPSDIKPEVLKVNSYEYPASGPIKDEFKGPINPSYLPAPKDPLPVPTPVPIPQDPAPQMDDMGMPPSDDTPTDDGDSHAGEINVVNGDEQGPPSDDHDHDHDHSHDHEHEHDPEHDHHHDHDHDYIPGKLYPTPPPGYIPEIPDDHDHDHHHDHFPIHDLKAFDYHDLVYDDHFFHHHHHHPTEPAPPPPPPTTEAPEPPPEPRVKKYSYFYIGRKLWYIPLYFTVTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTA0</accession>
    <name>T1GTA0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006925-RA">
        <property type="protein sequence ID" value="MESCA006925-PA"/>
        <property type="gene ID" value="MESCA006925"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973005_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006925-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14713" checksum="FC3F937670C2DEE5" modified="2013-10-16" version="1">MLGDKNCRGLVQVVQEMKDAAVEAYKGLKYIEENREDQDTWAKVVNKTREQAKKKTEENKAGKVRPDAIVVKINENATYAYIRQQMKSDTILKEVGEAISKIRRYRKESCSPNSIKLEENLYKFPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUR2</accession>
    <name>T1GUR2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02060772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007480-RA">
        <property type="protein sequence ID" value="MESCA007480-PA"/>
        <property type="gene ID" value="MESCA007480"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906652_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007480-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6985" checksum="7ACAA74E42D4DD1A" modified="2013-10-16" version="1">MADQKNDCEMNGGDEDFTQDVETNEQMNGNSDNQDESQNGNHENSNAGGRDDDRFLFDKILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUZ9</accession>
    <name>T1GUZ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="15">PPIase</shortName>
            <ecNumber evidence="15">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.</text>
    </comment>
    <comment type="function">
        <text evidence="15">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="15"/>
    <dbReference type="EMBL" id="CAQQ02191165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUZ9"/>
    <dbReference type="STRING" id="36166.T1GUZ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007572-RA">
        <property type="protein sequence ID" value="MESCA007572-PA"/>
        <property type="gene ID" value="MESCA007572"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_4_3_1"/>
    <dbReference type="OMA" id="SVWGQVI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000017">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="16">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007572-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20265" checksum="AF85E2CEBD00FD9D" modified="2013-10-16" version="1">MPTWNQIQNQLKNPNNPVVFFDIAVGTTEIGRMIFELFADTVPKTSENFRQLCTGEYKKDGVPQGYKGTSFHRVIKDFMIQSGDFVNGDGTGVFSIYGSAFADENFILKHDAPGLLSMANSGKDTNGCQFFITCAKCNFLDNKHVVFGRVLDGLLIMRKIENVPTGPNNKPKLPVTISQCGQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV05</accession>
    <name>T1GV05_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007579-RA">
        <property type="protein sequence ID" value="MESCA007579-PA"/>
        <property type="gene ID" value="MESCA007579"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657327_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007579-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8568" checksum="7991D29FAD1637D8" modified="2013-10-16" version="1">MLEEEERDRQRYRCLDLDEKGAKKPKKPASLKQIGGVDSAPNHAPNTLFLFLDKNKKVGVSTQPLTTHTLLFGKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV26</accession>
    <name>T1GV26_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein krueppel</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV26"/>
    <dbReference type="STRING" id="36166.T1GV26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007606-RA">
        <property type="protein sequence ID" value="MESCA007606-PA"/>
        <property type="gene ID" value="MESCA007606"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_2_4_1"/>
    <dbReference type="OMA" id="TRENFTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007606-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="60624" checksum="71BB28F4CD92CA29" modified="2025-10-08" version="2">MCRTCLKVLDLESENFHALQVYPFLLNLFNNFINPETIASCVNEAELPPNLCDDCFNKLQDISSFQKQCNETFLNFHILTQNDILIPLVKLEETFNGNSDFSDGEDKDFIDPLQTLEEDPSVALLSTNKNNKPKQKRGRKKKEKVPPPAATVPVQLPKKDPEQATTATTEATDAIPSLDIDLNNPPMEDIKEEDPGALFTNNELQIKVEQKDGGISSCEDNDNDNDAGDMTYNDNDNSQDDSGSDDNYSDKDENDDEDDEDKPRKKKAKKQPKKPTKFKCDVCPKSFYIEFRYKAHMREHMGLVPFECPHCDAGFSTFDSYKAHIKELHENVGVVYKCTEPKHADKYGNKKPFMCEECGKTFVHKSALTLHMYSHNEKEKPFPCEQCPKRFTSKSALNQHMLRHTGEKNFMCPYCGLQKYNKTDLKLHINYHTKERQWPCKYPGCTYVGNRSSAIGRHVRGVHEQIKNYQCKFCDRKFNKKETMKHHEMTHTGEKPHVCDICGWRFIQAIALKKHRETHSRSRSQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW59</accession>
    <name>T1GW59_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02165624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW59"/>
    <dbReference type="STRING" id="36166.T1GW59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008030-RA">
        <property type="protein sequence ID" value="MESCA008030-PA"/>
        <property type="gene ID" value="MESCA008030"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_3_0_1"/>
    <dbReference type="OMA" id="FRNIMTG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="11">
        <location>
            <begin position="265"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="560"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="562"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="596"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="621"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="645"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="692"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="360"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008030-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="753" mass="86013" checksum="7DEC56FD58133B54" modified="2013-10-16" version="1">MGRKAGYTEKEKKGPGRKARKQKPPTFNKPSFAPLEKEEGKLSHRQKQRQAKRLQKKQNKKPKVKQPKQVYNSDSEAEDVVEEEEVMSEEEEEDTPVLNGNGKVKGFSDENQSWLKPKQKKGAAPVEDEEEDDDDEEVEEEESEDEDEGKVKVGKLFDEEGDSDDEVPDDNFANKKLKKQEKEEAKLAEEEQQMSVLNQEIFKLPEEDGEEEKPVTLPEIQQRIKDVTLVLSDFKRYRQEGRSRQEYLEILKKDLCVYYSYNEFLMSKLIDMFPLTELMEYLEASEVARPLTIRTNTLKTRRKELAQALINRGENGLSSQVPLGATPEYLAGHYMIQGASSMLPVMALAPQENERILDMCSAPGGKSSHIASIMKNTGVLFANDANRDRIKAVVANLHRLGINNCIVSCEDGCKFRQVMTGFDRVLLDAPCTGTGVVAKDPSVKQSKSEIDVQRCYNLQRKLLLTAIDCLDAKSPSGGYLVYSTCSVLPEENEWVIDYALKKRNVKLVPTGLDFGTEGFTKYRQFRFHPSLNLTRRYYPHTHNMDGFYVAKLKKFSNTIPVDNDDDLEEEEKVEETVEETAVATEEKMEEDSEENTGEKSKEQSWQTKLDRCRNPAKKKKIADAEKKYVAKVFEKPVKVPKKKKAGQEETQKKSEEPEKKTEEKVNEKPPKTPKKNKKIEGKKSEPLLTESPESKPDSKPELKKQNNKNQNNSKKFNNKNKNHGNSEQNGKFKKDFQAKNNKNKKGTEKKSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWM7</accession>
    <name>T1GWM7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Magnesium transporter NIPA2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIPA family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWM7"/>
    <dbReference type="STRING" id="36166.T1GWM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008205-RA">
        <property type="protein sequence ID" value="MESCA008205-PA"/>
        <property type="gene ID" value="MESCA008205"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012349_1_2_1"/>
    <dbReference type="OMA" id="STWMPWF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF92">
        <property type="entry name" value="SPICHTHYIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30666" checksum="9B57E8E8E6058FB0" modified="2013-10-16" version="1">MMGVGEAANFAAYAFAPASLVTPLGALSVLVSAVMASRFLKEKLICLENWAEREIDDLEALFEKLIDPGFVLYIFLIISTCIFVAMFLAPKYGHTNVSVYIYLCSGIGSLTVMSCKALGLAIRETVSGKNNEFSTWMPWFLIIITVIFIAIQMNYLNKALDIFNTGIVSPIYYVMFTTLVIIASAILFKEFQHMSAEDILGDICGFLIVIVAVFMLNAFKDVDISLNDMRGIARPKHERLPTFDDDVYIRGSDKESNSRKVSYGTNEDFLMTRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWR6</accession>
    <name>T1GWR6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWR6"/>
    <dbReference type="STRING" id="36166.T1GWR6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008245-RA">
        <property type="protein sequence ID" value="MESCA008245-PA"/>
        <property type="gene ID" value="MESCA008245"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270468_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11604" checksum="E702094497E872DE" modified="2013-10-16" version="1">MVLCYGCAMNTNCALGTKYGKILERQLQKDFQIGFVETLAQEMASNEENTMDVFGSIAKIVKHNRSVKGEKKDWNAIVRSNTIHSNPIGTKRSSIIRRQQLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWZ9</accession>
    <name>T1GWZ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02045745">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWZ9"/>
    <dbReference type="STRING" id="36166.T1GWZ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008336-RA">
        <property type="protein sequence ID" value="MESCA008336-PA"/>
        <property type="gene ID" value="MESCA008336"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_2_1_1"/>
    <dbReference type="OMA" id="MYKVNSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000084">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF156">
        <property type="entry name" value="RH39904P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577218" description="Peptidase M14 domain-containing protein" evidence="12">
        <location>
            <begin position="35"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="134"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008336-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="427" mass="48761" checksum="BC2A53216DCA09FD" modified="2013-10-16" version="1">MSIKARSIVYVRLKSLRMWKLLCIFLGVFQASSGERVRYDNYHAYEVKAENMESLEFLRSLESSSERLLFLNNVQLIGQSAFVFVAPEKVSDFEDSLNKSNIPFELKSSNAQNIIQADEDDLLKPRSAEYDWTEYHDLEETYSWMESLAAKYPQVTVVEGGRTYEGQGNPGIVIEAGIHSREWIACTTATFMINELLNSSTTSVREVAENYDWYIFPHLNPDGFAYTRNLEIGDRMWRRTRSKQANGCIGVDANRNWDFHWREIGASGQPCLDDFAGPAPFSEIETKTFADYIRSLKGQIRLYISLHSWSQLLMYPYGHTQRLPVNVEDYRQITNAAIQAMAKRYGTKYIGGNIYDAIYPASGTSIDWVFGTQGVKLTFTYELRPSGHGAVTFSHDLPKEFIIPTAEETVDSIVALVNEAKKLKYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXE0</accession>
    <name>T1GXE0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008487-RA">
        <property type="protein sequence ID" value="MESCA008487-PA"/>
        <property type="gene ID" value="MESCA008487"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173832_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="11304" checksum="BB127F3A808B984D" modified="2013-10-16" version="1">MSEGVPLVAPLMKAKLEVMGNVGTKVKTAIGKKWESVKATGAAMGSIVAAPIHIVSKKAAIKLDALKGSVASKGAALKKTISQKLATTTTTTTTTLAPPSKDLHQIRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXF4</accession>
    <name>T1GXF4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXF4"/>
    <dbReference type="STRING" id="36166.T1GXF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008503-RA">
        <property type="protein sequence ID" value="MESCA008503-PA"/>
        <property type="gene ID" value="MESCA008503"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035857_1_1_1"/>
    <dbReference type="OMA" id="FTHAVYP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016263">
        <property type="term" value="F:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033:SF14">
        <property type="entry name" value="GLYCOPROTEIN-N-ACETYLGALACTOSAMINE 3-BETA-GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20759" checksum="DF9216DF52EE5A9C" modified="2013-10-16" version="1">YIVVENLRYLLYPYSPKTPVYFGHRFNAFQKEGYMSGGAGYILSQGALEVFVTKALNDEKLCRQDGRGAEDIELAKCLVNVGVQSGDSRDWTTLQGRFFPFAASSHLLMTYDKNYWFWKYQYYEPTLERQCCSDRSVAFHYIPPSELYLYDYMTYHLRPYGISEEIPTLPRKLRVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXG2</accession>
    <name>T1GXG2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXG2"/>
    <dbReference type="STRING" id="36166.T1GXG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008513-RA">
        <property type="protein sequence ID" value="MESCA008513-PA"/>
        <property type="gene ID" value="MESCA008513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070944_3_0_1"/>
    <dbReference type="OMA" id="KEPEGPC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21453">
        <property type="entry name" value="DLC-like_DNAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000002">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF2">
        <property type="entry name" value="DYNEIN AXONEMAL LIGHT CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 12" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13805" checksum="1DA585EF4D3E9225" modified="2013-10-16" version="1">MSVRNDGGLTKAITNRKMADDEKEKEGGDKRIVHVYPLVKHSDMIDEMRTEAVDLSITACEKFSSDYEQAAKVIKETLDKKFGTYWHVIVGEGFGFQVSYETNNLLYLFFAGNLAIVAWKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXK6</accession>
    <name>T1GXK6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXK6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008559-RA">
        <property type="protein sequence ID" value="MESCA008559-PA"/>
        <property type="gene ID" value="MESCA008559"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2747379_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8159" checksum="3D892C7729A4896C" modified="2013-10-16" version="1">MKHWYDEYKLELPKVDADAGQSPEKDVKKPKTKSKVESDESSGSELSSDEDDSMDAIPKRKKKEKVGFRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXY6</accession>
    <name>T1GXY6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="1">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02184473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXY6"/>
    <dbReference type="STRING" id="36166.T1GXY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008700-RA">
        <property type="protein sequence ID" value="MESCA008700-PA"/>
        <property type="gene ID" value="MESCA008700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024661_2_2_1"/>
    <dbReference type="OMA" id="VGDITHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF12">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="2">
        <location>
            <begin position="19"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008700-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="22102" checksum="D04B445FF1380D59" modified="2013-10-16" version="1">MSSELTPVQQFYKGKTVFITGASGFMGKVLLEKLLYSCSELNQAIILMRPKREKTPEKRLEEMFKLPLFQRINDEKPHVLKKVTLVQGDVTKDGLGLSESDLKLLTENVNIVYHMAATLKLEGNLTDAINMNLTGTQRTLNIAKQMKNLNVFIHLSTAFCNCDQEVMYEKVYEFHHKPEDLMRMAEWMDVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY28</accession>
    <name>T1GY28_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008748-RA">
        <property type="protein sequence ID" value="MESCA008748-PA"/>
        <property type="gene ID" value="MESCA008748"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3428612_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008748-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="20" mass="2333" checksum="080DF0019D607591" modified="2013-10-16" version="1">MEIPVAHSLFMKMANQSWLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY73</accession>
    <name>T1GY73_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02158779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY73"/>
    <dbReference type="STRING" id="36166.T1GY73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008795-RA">
        <property type="protein sequence ID" value="MESCA008795-PA"/>
        <property type="gene ID" value="MESCA008795"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2099666_0_0_1"/>
    <dbReference type="OMA" id="ANVAMKH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF187">
        <property type="entry name" value="CYTOCHROME P450 4AE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008795-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13522" checksum="9B271211475383F3" modified="2013-10-16" version="1">MPLIDTLLRSQIEGKPFTNQEIEDQVNTFLFAGHDTTSHTFSFFLYNVAKYRKVQERIFKEIIEVFGKCPENSELTIMKMNELKYMEAVIKESMRMFATTPFIESKLLANTVSRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYM1</accession>
    <name>T1GYM1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYM1"/>
    <dbReference type="STRING" id="36166.T1GYM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008954-RA">
        <property type="protein sequence ID" value="MESCA008954-PA"/>
        <property type="gene ID" value="MESCA008954"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822048_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099041">
        <property type="term" value="P:vesicle tethering to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039755">
        <property type="entry name" value="TBC1D23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13297:SF5">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13297">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="137"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008954-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="338" mass="38172" checksum="5B6A2EF64EB734DD" modified="2013-10-16" version="1">MVPITVRLKLWTLSDFVNWDLYFQSSDPFLVFFLSLIILINGREQILAMSSNSKEEVVKFLSNMPCALEIDDVVDFCSLAQYYSQNTPSSFKTEYLKALFGAHTEFPEKCLVSQALCLPVTVYELVENSTVELSSADSVRFFLVDCRPASQYNAGHLSTAFHLDCDLMLEEPIAFQTAVQGLLRAQRMAIEINSNAGGEHLCFMGSGRMEEDQYAHMVVASFLQKNIQYVSLLSGGYKAIHDYFGEHMTDCLEDHDSRKCLVCQKAEFTKQSPPSTSKSVENLKPQSDFFSKLKEKSAEVKGKLLDIIVNPNVAYLLSQRILQFKEGQRPLQRPLFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYN7</accession>
    <name>T1GYN7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYN7"/>
    <dbReference type="STRING" id="36166.T1GYN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008973-RA">
        <property type="protein sequence ID" value="MESCA008973-PA"/>
        <property type="gene ID" value="MESCA008973"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3020305_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008973-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="56" mass="6241" checksum="EBF8C2F9FED2A1E5" modified="2013-10-16" version="1">MACICCKSKPEMSPESKQKESKSPRNKQDFEGTFMTEAKDWAGELISGQTTTGRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYT6</accession>
    <name>T1GYT6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02099267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYT6"/>
    <dbReference type="STRING" id="36166.T1GYT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009023-RA">
        <property type="protein sequence ID" value="MESCA009023-PA"/>
        <property type="gene ID" value="MESCA009023"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019281_6_3_1"/>
    <dbReference type="OMA" id="IKPCRIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF3">
        <property type="entry name" value="PROTEIN GOOSEBERRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009023-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8042" checksum="BC36E2D245966AAC" modified="2013-10-16" version="1">MISRQLRVSHGCVSKILNRYQETGSIRPGVIGGSKPKVTSREVEDRIEDLRKSNPGIFSWEIREKLIKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYY3</accession>
    <name>T1GYY3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02377113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYY3"/>
    <dbReference type="STRING" id="36166.T1GYY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009071-RA">
        <property type="protein sequence ID" value="MESCA009071-PA"/>
        <property type="gene ID" value="MESCA009071"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_3_1"/>
    <dbReference type="OMA" id="CAETNFP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000054">
        <property type="entry name" value="Complement C1r subcomponent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009071-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6242" checksum="CB9AB44D267EF185" modified="2013-10-16" version="1">MLCAAAENKDACQGDSGGPLVANNQLVGVVSWGIGCARAQYPGVYSSVANLRDWVIENSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYZ6</accession>
    <name>T1GYZ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YPP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYZ6"/>
    <dbReference type="STRING" id="36166.T1GYZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009084-RA">
        <property type="protein sequence ID" value="MESCA009084-PA"/>
        <property type="gene ID" value="MESCA009084"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1135766_0_0_1"/>
    <dbReference type="OMA" id="CPLFCAT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009084-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="23481" checksum="6453C963ED832034" modified="2013-10-16" version="1">NNSLGFRTFIERLFRKALKFSYGEQHIWKQYGISLAWLGRYKHSLRVFEEATRLIPNDLHCHHFCPLFCATSLFHEGLHHSKQDLAKELKGLRPSRSQLYVGIGYQQIANQSNSDRENYHKLALGAFQKAVEDGENDHLSAYYLALEYTLLNNIPEAMHNIRCALALRADHSPSLHLFTLILTATEKHFEVLNVVEDALEEFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ03</accession>
    <name>T1GZ03_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02152833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ03"/>
    <dbReference type="STRING" id="36166.T1GZ03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009092-RA">
        <property type="protein sequence ID" value="MESCA009092-PA"/>
        <property type="gene ID" value="MESCA009092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3335356_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="45" mass="5030" checksum="841FCB87B5CC4DC3" modified="2013-10-16" version="1">MILLEINNRIVEETLTIKFKNALAGSKPESIDVTIADFDGVLFHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZC8</accession>
    <name>T1GZC8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZC8"/>
    <dbReference type="STRING" id="36166.T1GZC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009227-RA">
        <property type="protein sequence ID" value="MESCA009227-PA"/>
        <property type="gene ID" value="MESCA009227"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1628938_0_0_1"/>
    <dbReference type="OMA" id="FHNLDIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009227-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18314" checksum="A7FB5DB31AEEA984" modified="2013-10-16" version="1">MEENETVNPSAKAQRPSITSYSRGNASSSYQRLTTATNTARPSTAVRAVGYTGESSKSFEKLLNQKMKIQNDSAINNSDFKDNEPQIKYKHLENKVTNLLEQSIILSASSPPNFIEALNKAKEASVLDSALLRMRDQNNETSFHNLDITYSFYSESLYDDEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZH4</accession>
    <name>T1GZH4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZH4"/>
    <dbReference type="STRING" id="36166.T1GZH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009275-RA">
        <property type="protein sequence ID" value="MESCA009275-PA"/>
        <property type="gene ID" value="MESCA009275"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3016600_0_0_1"/>
    <dbReference type="OMA" id="MRIQRND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051846">
        <property type="entry name" value="SH2_domain_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127:SF32">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009275-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6635" checksum="D636786D4F474695" modified="2013-10-16" version="1">MHMRIQRNDNGQYILGQFSRPFDSIPEMIRHFCLNRLPVRGAEHMCLIEPVIVQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZP7</accession>
    <name>T1GZP7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009351-RA">
        <property type="protein sequence ID" value="MESCA009351-PA"/>
        <property type="gene ID" value="MESCA009351"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545213_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009351-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9216" checksum="026858164AEE11F5" modified="2013-10-16" version="1">MFEGYAKSKNQFTGIGSGMGAIKVGEIWFITVLGLICNLLTLFNGLHSAYRELALVPISAIISDIGSRFQIPYLYKYLKLGPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0C5</accession>
    <name>T1H0C5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02016725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0C5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009600-RA">
        <property type="protein sequence ID" value="MESCA009600-PA"/>
        <property type="gene ID" value="MESCA009600"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388719_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588640" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10565" checksum="549A66225896ADE8" modified="2013-10-16" version="1">MLFVKSILFIYLICGILGSPMPQCVFECLQTFRDGHTRTVCFRNMNNGFVFGYQSNCEVLRISCIKHSLDKYQLVEQGICVGQAPSINPLQGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0T9</accession>
    <name>T1H0T9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0T9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009780-RA">
        <property type="protein sequence ID" value="MESCA009780-PA"/>
        <property type="gene ID" value="MESCA009780"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476785_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009780-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="19362" checksum="C153A3C8BE0557FC" modified="2013-10-16" version="1">MDSAIDTLSTTSEHSVASSIESTTAAMNSSQNSSKRSHSPYNNNTSHNNHNTYHSNGGSSAGGGRMRPSSCSPQSSPNRHPNSPGGNSASRNVETHSAMIQQMQHQLSPPPQGAGTPNGMPAGLPPRMPHLPPHSLGLLNSLQMMHHASPLELMAAAHLKCHRGLTTAHHPYPHPTQVLMSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H248</accession>
    <name>T1H248_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 5">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 5"/>
    <dbReference type="EMBL" id="CAQQ02194660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H248"/>
    <dbReference type="STRING" id="36166.T1H248"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010280-RA">
        <property type="protein sequence ID" value="MESCA010280-PA"/>
        <property type="gene ID" value="MESCA010280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_1_1_1"/>
    <dbReference type="OMA" id="YTMNFIR"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000035">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleotide reductase large subunit" evidence="6">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="664" mass="75008" checksum="B2D91842057ABC1F" modified="2013-10-16" version="1">MLMRVAVGIHGNDIDAAIETYNLLSERYFIHASPTLFAAATPRPQLSSCFLLTMCSDSIEGIFKSVEQCAMISKSAGGIGLNVHCIRAKGTAIAGTNGTSNGLVPMLRVFNNVARYVDQGGNKRPGAFAIYLEPWHSDIFEFLDLKKNTGKEENRARDLFYALWIPDLFMKRVEADEDWSLMCPHKCPGLHDVWGEEFEKLYIKYENEGKANRKIKAQQLWYAIIESQVETGTPYMLYKDACNRKSNQQNVGTIKCSNLCTEIVEYSAPDEIAVCNLASIALNQYITADQYSAPDEIAVCNLASIALNQYITADRKYDFPKLKEVTKIITRNLNKIIDVNYYPLPEARKSNLRHRPIGIGVQGFSDALILMRYPYESEKAQLLNQQIFETIYYGALEASCELAEKEGTYETYEGSPVSKGILQYDMWDKTPTDLWDWQALKDKIKVHGVRNSLLIAPMPTASTAQILGNNESFEPYTSNIYNRRVLSGEFQVVNHHLLKDLTELDLWDDDMKNEIISKRGSIQEIERIPAHIRELYKTVWEISIKSTIQMAADRGAFIDQSQSFNIHVAEPNYGKLTSMHFYAWKLGLKTGMYYLRTKPAANAIQFTVDKNKSAVNVTNGTSSPADTSVLNESMSSLNLSERERRMAEMVCSLENKDNCLMCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2J9</accession>
    <name>T1H2J9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2J9"/>
    <dbReference type="STRING" id="36166.T1H2J9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010447-RA">
        <property type="protein sequence ID" value="MESCA010447-PA"/>
        <property type="gene ID" value="MESCA010447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_656540_0_0_1"/>
    <dbReference type="OMA" id="ANGWPAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="419" mass="42771" checksum="C3BC5573F23A142F" modified="2013-10-16" version="1">NYPPRPQYYGPGAPPPGAPTSQPGSHGPPPPGQYQGRQMPNHQYPPGGYQQSWVPPSPGPMGNHVQGKGTPPPPGPTGSPRPVNYLKQHLQHKGGPGPGPAGYMGSQTPPPPPPQGYGNGPGMHMGGPMGPPHHMGPPSQPMVPPSSLSSTTTTTTSSGHPVTSVITTGPDGATIDEASQQSTLSNASAASGEDPQCTTPKSRKNDPYQSHLAAPITSPGAGAPGTPGPYPPPHTGSAGTGGGTGGGGSDDFDMRSPPPTWQGSQGPYRGGPYANAPPQSQQWPTGPRSQAPGGPQGSANQQPIGPGGQWDQHRYPPQSQQPYPQPGAPGGPQQQWGNMPSPQQQPAQQPPSSTPGSPLRPPNQQQRMPLPPGGKPFNMPPPPQNMPPQTGGPQNKMAPHHMPPHPPMGPPPQGANAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3R1</accession>
    <name>T1H3R1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proline-rich transmembrane protein 3/4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02377631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3R1"/>
    <dbReference type="STRING" id="36166.T1H3R1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010895-RA">
        <property type="protein sequence ID" value="MESCA010895-PA"/>
        <property type="gene ID" value="MESCA010895"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_970725_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059081">
        <property type="entry name" value="PRRT3-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052836">
        <property type="entry name" value="PRRT_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35578:SF6">
        <property type="entry name" value="PROLINE-RICH TRANSMEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35578">
        <property type="entry name" value="PROLINE-RICH TRANSMEMBRANE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25987">
        <property type="entry name" value="PRRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Proline-rich transmembrane protein 3/4" evidence="9">
        <location>
            <begin position="49"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010895-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="287" mass="31587" checksum="7128477D2E7620B1" modified="2013-10-16" version="1">MRPLTVYVGGGVHVTLCITLHYVESYTTGRFLSSVAPTSSGAAAAPPIKILSLICQLFYIFICLFLGGLYMTLYPRLKRVLAAKNQNYIHEYSSFLYAIHITISTALLFVLMAALQMFGAFTISTARRASIDKDWLQWGYQFSLRLIELAIILLVSWVSGLKVGVSVEHELSTNHRHFKEIEPPSHYSEPMEFSRRVYDVYEIGATAGGPTTESSSSGSFGSRRRNDDVVSNCNAERTRLTVLGTAAQGLAPTPPPPPTTNGSMLVAECGFVRFRNLENMNFVGAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3V3</accession>
    <name>T1H3V3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02192551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3V3"/>
    <dbReference type="STRING" id="36166.T1H3V3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010946-RA">
        <property type="protein sequence ID" value="MESCA010946-PA"/>
        <property type="gene ID" value="MESCA010946"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="OMA" id="WITARME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF106">
        <property type="entry name" value="CYTOCHROME P450 4G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010946-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23391" checksum="DDE0E99B265ED871" modified="2013-10-16" version="1">MPSDDMIVNTEGHDTTAAGSSFVLCMLGLHKDIEARVMKEIEGIFGDSKRPCTFADTLEMKYLERVILEALRMYPPVPLIARKVNEDVRLTTGKYTIPKGATVVIPQLFIHRNPEIYPNPMKFDPDNFLPERTQNRHHYAFIPFSAGPRSCVGRKYAMLKLKVLLSTILRNYKITSPVKEEDFKLQADIILKRAEGFQLLIQPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4B2</accession>
    <name>T1H4B2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemocyanin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4B2"/>
    <dbReference type="STRING" id="36166.T1H4B2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011120-RA">
        <property type="protein sequence ID" value="MESCA011120-PA"/>
        <property type="gene ID" value="MESCA011120"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067272_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.10">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005204">
        <property type="entry name" value="Hemocyanin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036697">
        <property type="entry name" value="Hemocyanin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF24">
        <property type="entry name" value="GH04080P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03722">
        <property type="entry name" value="Hemocyanin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48050">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemocyanin N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011120-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13496" checksum="09D708174E016114" modified="2013-10-16" version="1">MDFCMEIGRYDEFSLFIPNHTRIAGALIQIFIEQETVDNLLSVAARIRDKINPMLFNYALSVAILHRPDTKLLPIPLLANIFPEKFLHSLVFNEARKKGTKLLATSETESESVPVIKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4B7</accession>
    <name>T1H4B7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4B7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011126-RA">
        <property type="protein sequence ID" value="MESCA011126-PA"/>
        <property type="gene ID" value="MESCA011126"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560899_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011126-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9683" checksum="77BEADE179C4AB47" modified="2013-10-16" version="1">MNRNEDELDPEDVDLDEAYTNDTRSRCKLPQILNCSLCFALLLHSSEDLDTDTEFEPVEDMALETRSRCMPFFERSYWRPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4P6</accession>
    <name>T1H4P6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02027276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4P6"/>
    <dbReference type="STRING" id="36166.T1H4P6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011262-RA">
        <property type="protein sequence ID" value="MESCA011262-PA"/>
        <property type="gene ID" value="MESCA011262"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049543_10_1_1"/>
    <dbReference type="OMA" id="QDRNEFA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF5">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="24"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011262-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8868" checksum="5ECDBEBAFCF71981" modified="2013-10-16" version="1">MTPLGAKDEDGNSISQSGHHGLSKKQRKARTAFTDNQLQTLEKSFERQKYLSVQDRMELANKLGLNDTQVKTWYQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4R7</accession>
    <name>T1H4R7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4R7"/>
    <dbReference type="STRING" id="36166.T1H4R7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011284-RA">
        <property type="protein sequence ID" value="MESCA011284-PA"/>
        <property type="gene ID" value="MESCA011284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003736_5_0_1"/>
    <dbReference type="OMA" id="ICIQGKQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="186"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="475"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="608" mass="68892" checksum="528333647CAFA1D3" modified="2013-10-16" version="1">MSNFKEINDLIEKLCKHLVGPSKQKEALKHVTQYFSTAPNSFNTSTQYDETYLVSRIANHIRRTRSKSDVGLFLSLHEQLKSSQIESKRRSALLTFLYFMSDNQSSKSTISSASNGSQLYSSNHSSILTSTKHSMYQRLRDSSEGPSTIQLRSPSDSDISAQSSITNLDSNNVEDNKVRELSDDIVQNVINSFTGIQGKYLKKDINGNFKLDTKEAKSLQITQAGTILRLSEIGFYHDQVETFSNPKSGKTALGLMGQGFVSALQNELTSFYGIVAVLQDHYNRQRKLSLHDWKECPEKLTLIKLMVWSGEPLQRLQWLATIAEACQEKKGGILASTVYKFLSNGDPVVKSIVKDLLLAICRPLQQMMSRWLMEGEIIDPHGEFFIDVVHEVGSENLWKEKYLIKETMVPVFMSHNMVNKILGTGKCINFLREICNDKSCIHGKEELKEYLDSEKSVYLSTSKKVLDIMMVTHRLLDHMHVIRQYILFGKGDFIQILIENLKSKLDRPASELIRCDLTSVLDASLRATCTSQSESALCDNLEFKTFTPLAGDSSWEIVALFYNVKGPLATILSSNNDISNIFKTLWRIKHMDFLLSKKIWIEQIGNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4Z4</accession>
    <name>T1H4Z4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4Z4"/>
    <dbReference type="STRING" id="36166.T1H4Z4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011367-RA">
        <property type="protein sequence ID" value="MESCA011367-PA"/>
        <property type="gene ID" value="MESCA011367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078927_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13656" checksum="F0A471B549C1E861" modified="2013-10-16" version="1">MFSTLCKQHSSVSGVVKMCDGLWFREKDLEDDHACILMKSDPVVSYRETVKEKSNQMCLSKLPNNLLMPDDLAEFNDNGKVSSKRDFKGRARYLDEKYDYDVTEARKIWCFDPGSTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H549</accession>
    <name>T1H549_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H549"/>
    <dbReference type="STRING" id="36166.T1H549"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011424-RA">
        <property type="protein sequence ID" value="MESCA011424-PA"/>
        <property type="gene ID" value="MESCA011424"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880596_0_0_1"/>
    <dbReference type="OMA" id="CIRAAND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF6">
        <property type="entry name" value="SITS-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15235" checksum="0793F6E7BE14E5C4" modified="2013-10-16" version="1">NRCYTLKWQALGEGVFPTDCFELSRDGGLWFGGGLTKNADWNLNTANFSFAPFITGDSKVYQFGNALKRYFLNSRGVAIEVSDKTPFHLSIKQGTKQNGAYDNTLCIRAANDEFAFVNKLTPLPELEYKVCIAEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H618</accession>
    <name>T1H618_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H618"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012151-RA">
        <property type="protein sequence ID" value="MESCA012151-PA"/>
        <property type="gene ID" value="MESCA012151"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011608_2_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12414">
        <property type="entry name" value="RRM2_RBM28_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000182">
        <property type="entry name" value="RNA-binding motif protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="393"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="479"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="530"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012151-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="550" mass="63443" checksum="3B0662185C7AB5EE" modified="2013-10-16" version="1">TKEKKEPFRLEKEKEDRERRQKKRARIIIRNISYKSTEESLNKHFSQFGKIEDLKLLKRPDGKLVGCCFIQYEKINEAAKAILKANNKEFLGRPVYVDWAVGKNEYTRNQNLQKKPEVKEEIKEEIKEEENESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEPHPAMSRDEVQDMSDKKSKKDDGKDSRNLYLAKEGIIMTNSKAAEGVSASDMKKRHELERVKTQPHECRVMRDNKPTAEHPQGMSKGFGFVSFETHLNALTALRKLNNNPNIFGXXXRPIVAFSIEDRSVHNIKQKRLEKSRSNNPNSKKNQTKRNADKRESESSKDSKAEVQKQIKKLEEIRTKSQIKKKSVEVDANYVGEEAKPGSFIKMRSMKKIRAQAQEHKQPVKNGKTQGKEGFKLRGSKRIKPKQKIQKDTPEFTNLVSKYKRMLDRQNEPAQPKVKRKKWYTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H645</accession>
    <name>T1H645_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">General transcription factor IIF subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transcription initiation factor IIF subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H645"/>
    <dbReference type="STRING" id="36166.T1H645"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012178-RA">
        <property type="protein sequence ID" value="MESCA012178-PA"/>
        <property type="gene ID" value="MESCA012178"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047858_1_0_1"/>
    <dbReference type="OMA" id="PIADNCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015849">
        <property type="entry name" value="TFIIF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="9">
        <location>
            <begin position="166"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="27735" checksum="05DD18CF31B0D783" modified="2013-10-16" version="1">ELDLSNAGRGVWLVKVPKYIAQRWEAAPSHVDVGKLRISKVAGQKAQVSLTLSEDVVNLEPEVKIPTEHKLDVSVVTKQTLGVFSHVIPTAKEESEKLYMEGRIVQKLECRPVADTSYMKLKMESFKKAAVPQKKVQAIDKIVQNFKPVKDHRHNCPEKKAEGKKARDDKNFVMDMLFNAFEKHQYYNIKDLVKITKQPIGYLKEILKEVCDYNMKNPHKNMWELKKEYRHYKADDNKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6F3</accession>
    <name>T1H6F3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6F3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012286-RA">
        <property type="protein sequence ID" value="MESCA012286-PA"/>
        <property type="gene ID" value="MESCA012286"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2216317_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF26">
        <property type="entry name" value="SIDESTEP III, ISOFORM O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012286-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11893" checksum="07FBC927B1004E1A" modified="2013-10-16" version="1">FDVRGRPFEKALYWSDTNSFGPRAYFVTHMQPATLTVENIQLDDEGXXXXXXXXXXXXXXXXXXXXXXXXPPHQILVYDASGRDVAGAVGPLLEGDNIVLTCEVRGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6H3</accession>
    <name>T1H6H3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L35a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6H3"/>
    <dbReference type="STRING" id="36166.T1H6H3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012306-RA">
        <property type="protein sequence ID" value="MESCA012306-PA"/>
        <property type="gene ID" value="MESCA012306"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100745_0_2_1"/>
    <dbReference type="OMA" id="NSGMFHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012306-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12836" checksum="1BE512BF1FF132E4" modified="2013-10-16" version="1">KAANTERYIKPANSLKCHGLLYAKAPFTEYKRDLRNQHENHVILNIERTRRKEHGHFYIGELCVYVYKTQTRKCAPQHPERNTKLRAVYGKATRFHGCVRAHFIFQDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6V5</accession>
    <name>T1H6V5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Synaptosomal-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6V5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012437-RA">
        <property type="protein sequence ID" value="MESCA012437-PA"/>
        <property type="gene ID" value="MESCA012437"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096939_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000928">
        <property type="entry name" value="SNAP-25_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
        <property type="entry name" value="SNAP-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SNAP-25" evidence="3">
        <location>
            <begin position="58"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012437-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="15972" checksum="27BEBA73FAD5E2F3" modified="2013-10-16" version="1">SKEAGIRTLVALDDQGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSQSFKEDDGTWKGNDDGKVVNNQPQRVDDRNGMMAQAGYIGXXXXXXXXXXXXXXXXXXXXXXXXXXXXAVSVETRIEVANQKAHKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7E9</accession>
    <name>T1H7E9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trehalose-6-phosphate synthase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7E9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012686-RA">
        <property type="protein sequence ID" value="MESCA012686-PA"/>
        <property type="gene ID" value="MESCA012686"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_7_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000113">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000324">
        <property type="entry name" value="Putative Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012686-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="58110" checksum="A5245BD63518DCCE" modified="2013-10-16" version="1">VVSVNLDPKIFDSYYNGCCNKTFWPLFHSMPGRATFSSDHWRNYVIVNKHFANRTIEALEKHYKQIKWKIVWIHDYHLMLASNWVREQAEERKLDCRMAFFLHIPFPPWDIFRLFPWSDEIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTKMKIVLGVDRLDYTKGLVNRLQAFESLLVKYPEHRGKVSLLQISVPSRTDVKEYRELKEEVDQLVGRINGRFTTANWAPIRYIYDYVGQAELAALYRDAAVCLVTPLRDGMNLVAKEFVACQINKVPGVLVISPFAGAGEMMHEALQCNPYEIDDAAEVIHRALTMPEDERILRMSRLRSREQECDVNHWMRSFLKAMGTVEFDDIGTTTMQPVSIDDFDDYLLKYIGYNHKVAILLDYDGTLAPIAPHPDLAILSPETKNILYKLSNHSEVYVAIISGRSVDNVKKMVGIEGITYAGNHGLEILHPDGSKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAD9</accession>
    <name>T1GAD9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000194-RA">
        <property type="protein sequence ID" value="MESCA000194-PA"/>
        <property type="gene ID" value="MESCA000194"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415766_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000194-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="11033" checksum="1A47A53C851353FF" modified="2013-10-16" version="1">MTQKNFFDRSKHQLGSTLKKQGLAFEGKDESEIFFGHPKYNDKFGLKELIQGKQQIYFSHKFCKIWLHLENILKKPHLLSKSYWQIMPFNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAW1</accession>
    <name>T1GAW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197592">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAW1"/>
    <dbReference type="STRING" id="36166.T1GAW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000376-RA">
        <property type="protein sequence ID" value="MESCA000376-PA"/>
        <property type="gene ID" value="MESCA000376"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040462_0_1_1"/>
    <dbReference type="OMA" id="QECGIIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF203">
        <property type="entry name" value="FI18611P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="41525" checksum="22A20D751093C861" modified="2013-10-16" version="1">MVNSTEVLNEFLHHLQHSHSHQHHHHAEDHDVHVARHDDGEEEEDTGSVLIAKVTAMVVLFCASTLCGSIPFILNRWFHWTDENSKSPRSAMVVKCLLYFGGGVLLCTTFLHLLPEVKENIEELQNDGTLPELRFHLAEFLTCMGKVEKQFHEEGEESESVPPTSQQDLIGNDLENQKYQMQHQEHNHEHNHHSDMQTHDCSAEGHHSHLPTDLDLGSDMIVSSLRGLLVVLALSIHELFEGLAVGLEPSSSKVWLMFGSVSAHKLVLAFCVGVELIVTKTRTWLAMVYVLTFAIVSPLGIGIGILISNHDNSSATAIPSAILQGLATGTLLYVVFFEILSKDKSGLVAYIAFLVGFFVMFGIQLSSKTGILIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBC9</accession>
    <name>T1GBC9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02086728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02086729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBC9"/>
    <dbReference type="STRING" id="36166.T1GBC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000561-RA">
        <property type="protein sequence ID" value="MESCA000561-PA"/>
        <property type="gene ID" value="MESCA000561"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297242_0_0_1"/>
    <dbReference type="OMA" id="IQIRMEE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF36">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-LIKE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588161" description="Myosin tail domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="17"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000561-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="25160" checksum="8D82FF22110FF8CA" modified="2013-10-16" version="1">NVFFLQIKCCKFLLQLSDAYEEVEEQRQVVGQWKRKTQKMTNEMNDLRMLLEEQNGRNNLLEKKQRKFDAECQSLQDAARQERQAKDRLAREKDVLIAEKFTLEQSLADARLELDLKEEKLSSLQLDLEEMAFGGGTEEEVANLKRSKMDLDRRVKEQEEELDEMAGQVQLLEQAKLRLEMTLETMRKEARREAQQRDEELEEARGSAYKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBP1</accession>
    <name>T1GBP1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBP1"/>
    <dbReference type="STRING" id="36166.T1GBP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000677-RA">
        <property type="protein sequence ID" value="MESCA000677-PA"/>
        <property type="gene ID" value="MESCA000677"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028700_1_0_1"/>
    <dbReference type="OMA" id="HACASIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039136">
        <property type="entry name" value="NUFIP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309:SF0">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION PROTEIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000677-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="410" mass="46541" checksum="AFF5E9A4B548EAEF" modified="2013-10-16" version="1">MDKSLPSPAFALPSPSFKAPEKSTKNLPHFLSPNGIQQLSRNQQPPPMYGPRGATIPPKHFNEYGKGATQEQKEYCEACDWEFNSLAALKSHEAEHEVCAVEGCNFKAITKIVDKHIEMAHKTGLFDQIKKLDSPEEIAKWREARKKRYPTVQNVQLRQQMQKEKFQRGEKLEPRKNRFGKNNDRTQGPQNSSNSNNFNNSKKRPNQNCDKSAKVRKVDSQKKSPVKKDITSKETEKMQDSSSIPKKETVTEERDEDMWNGNIPMFRGTSKWKDYKHVGNNVLSGLLGDYGSDSDEENDENVESEKEGTPSQNEKQEISNGVNSITDVPLEETVESKVKEIKKDLNSPEKLPSECPPNKDSEQSSTKNLESKDESEEAPLECPIAHEPEPGPQECSIKVLYDRQKRKNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC92</accession>
    <name>T1GC92_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02181900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC92"/>
    <dbReference type="STRING" id="36166.T1GC92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000898-RA">
        <property type="protein sequence ID" value="MESCA000898-PA"/>
        <property type="gene ID" value="MESCA000898"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1890970_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000898-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="14729" checksum="5F684D0152A137CD" modified="2013-10-16" version="1">SLLALPSTREKRSKKVGEEILELLTKPTAKERSLAEKFKSHGGAQVMEFCPHGTKVECLKAQQAKEEMLTARNCLVSRPGLFSPASIRFELKSSTIFLATPKAPFIVVKMTPVGPVPSQPAAYKPLSMTSFSPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCC7</accession>
    <name>T1GCC7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000937-RA">
        <property type="protein sequence ID" value="MESCA000937-PA"/>
        <property type="gene ID" value="MESCA000937"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1215984_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000937-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="25190" checksum="BB249FF813ACB58E" modified="2013-10-16" version="1">MSLEITYVKEYTFHDTNAKIYHHNTNPNTYELLKTTSCTGECFNPKNSDTYNLIDHEDFIDNDLDETASNLDISASVVAKRRLRKLTAANKQHNADDRDALRHGGLYRPPKGHQSNSSTATASATTVLQTSTNTIGGGGSRGAAQTTTTNNTPTTATTSATTNCSRNNHHNCTCNSIDSNHDSSDFSMNLLNQNNSSTEVPQLQQQQLHHLLQPQQLQILQTTQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCP8</accession>
    <name>T1GCP8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001068-RA">
        <property type="protein sequence ID" value="MESCA001068-PA"/>
        <property type="gene ID" value="MESCA001068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530059_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9426" checksum="59F2636797E7BE74" modified="2013-10-16" version="1">MCLVVNEDPPMRGGDISSLFDNAIVKEFISQSEISDIESHWNMCPEVDSSTPTLPTIYGPNPESLTRQTLRCDKTPEKPQMSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD58</accession>
    <name>T1GD58_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02029832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD58"/>
    <dbReference type="STRING" id="36166.T1GD58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001239-RA">
        <property type="protein sequence ID" value="MESCA001239-PA"/>
        <property type="gene ID" value="MESCA001239"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663195_0_0_1"/>
    <dbReference type="OMA" id="MGSHARL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11394">
        <property type="entry name" value="bHLHzip_SREBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000098">
        <property type="entry name" value="Sterol regulatory element-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062:SF1">
        <property type="entry name" value="LP12374P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="185"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="146"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="286"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001239-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="415" mass="45682" checksum="617B51DDBF07E283" modified="2013-10-16" version="1">MMGNNISPVHHPQPQPQIFNPMLARNYQQQNQINRQQQQPQQQSPQVQHILPKQQMPSPTPTHIQQQPHSPSPIQIQPNQSPIMYATAPAKSPQMATQQTTPTTIVPQIVALDNKIILQTSNPTLMFTTENSSNGSLLLTTATSSPSAQRFSTEGKIAINRNHSSSTSTSSSNSSSAPQPKVKEVKRSAHNAIERRYRTSINDKITELKNLVVGESAKLNKSAVLRKSIDKIRELSRQNYELKAEVQRLQRELVNRDGLKVKDLLHVNLKKRKTPLDVMDVCLGGSVNGPSAKRPNENNVPLTPPRSDESNPSLSPTHSDISSLSMPSSPLFAENSSMNGSVKDMSSNEDELISIPSVRGMGSHARLTLCMFMFAMLAINPFKAFLGDDGASSYTAEEDVGGIRRNILSVDNYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDE3</accession>
    <name>T1GDE3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113916">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GDE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001328-RA">
        <property type="protein sequence ID" value="MESCA001328-PA"/>
        <property type="gene ID" value="MESCA001328"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_0_1"/>
    <dbReference type="OMA" id="ERTPILW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="191"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001328-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="275" mass="28739" checksum="DBF41CB935195BAC" modified="2013-10-16" version="1">SALHYCCVKQTEDNSNSSLVAAASVAIAAPELLESADEDGFTPLHLAIIQGNLAMVNLLLANKADVNAVDNEGHSVVHWATVCGEIEALRSVLQAGANVTTPDINGGSPLHYAAQMCGANYENKVGQAPSLSLDILGILLTHPLSSVXXXXISIIGYSWNTFNSSTFISGSAKAILALVKKGARVESADKDGLTALHCAASRGHTECIDTLISLCGAHTDLIDNNGCTALHYAVTLGHADATSRLLDLEADPNRQDRKGRTPAHCGCSKGQLETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDF4</accession>
    <name>T1GDF4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001340-RA">
        <property type="protein sequence ID" value="MESCA001340-PA"/>
        <property type="gene ID" value="MESCA001340"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690640_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001340-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8765" checksum="AEEABEA32C8F1648" modified="2013-10-16" version="1">MRDRNCGSVSEDRWGKRSSWKHKFGVSLPLEEKPGTAWKEREEEVANEPEGRELGIPNWRVTARDRNTWKQYCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDQ4</accession>
    <name>T1GDQ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CUT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02156285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDQ4"/>
    <dbReference type="STRING" id="36166.T1GDQ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001447-RA">
        <property type="protein sequence ID" value="MESCA001447-PA"/>
        <property type="gene ID" value="MESCA001447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_535638_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000010">
        <property type="entry name" value="Cut-like homeobox 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000027">
        <property type="entry name" value="Homeobox protein cut-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="11">
        <location>
            <begin position="82"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="CUT" evidence="11">
        <location>
            <begin position="405"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="509" mass="58116" checksum="19840035A8E52141" modified="2013-10-16" version="1">MKEKHQESENYQNFDRNHLQNIVPNIHFLSSLQYQFPERNHFRFADDLQLPPGTSLAGRLGDSLIPKGDPMEAKLQEMLRYNMDKYANQSLDTLHISRRVRELLSVHNIGQRLFAKYVLGLSQGTVSELLSKPKPWDKLTEKGRDSYRKMHAWACDDKVVLLLKSLIPKKDSNQPFGKSEDCQNEEKKSDNNMEDYSRDNFSKIYQEEFAKIMRTQSFLFPHFFNRNIPSNLSNESIRLNCDNYQKEVMKYKQNNLISSNQKIGEFLGQILPNDAQDLSMPKDINVNKDVKTSSQVDTSKQKLINETIKSEDCVDKSKSNVHYHPSEMRTSTPITPNASSGEPSSLGNSILAPSAIPSTEDLSSSASPLQRMASITNSLISQPPMLPSHNTQQRPSKAILPPITQQQFDLFSNLNTEDIVRRVKESLSQYSISQRLFGESVLGLSQGSVSDLLARPKPWHMLTQKGREPFIRMKMFLEDENAVHKLVASQYKIAPEKLMRTGITVEIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDW0</accession>
    <name>T1GDW0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02117559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02117560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001511-RA">
        <property type="protein sequence ID" value="MESCA001511-PA"/>
        <property type="gene ID" value="MESCA001511"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888330_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001511-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7113" checksum="86656C45FD033FBC" modified="2013-10-16" version="1">MFAPLNEQYTRRVSAVQRSGTYTGSHPAIEVCSAPFEVAKRSKTWTTTARVVLNMYGQRDIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEN6</accession>
    <name>T1GEN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-hydroxy-3-methylglutaryl-coenzyme A reductase</fullName>
            <ecNumber evidence="3">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02129656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GEN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001805-RA">
        <property type="protein sequence ID" value="MESCA001805-PA"/>
        <property type="gene ID" value="MESCA001805"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095456_0_0_1"/>
    <dbReference type="OMA" id="CIISATT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001805-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="27933" checksum="E74FCFE459CBE821" modified="2013-10-16" version="1">MNACCENVIGYLPIPVGYAGPLLLDEKEYYVPMATTEGALVASTNRGCKALSVRGVASYVEDVGMTRAPCVKFPNVAKAACAKRWIEDPENFKSIKKEFDSTSRFGRLKDIFVAMDGPLLYIRFVALTGDAMGMNMVSKGSEMALKCLQLHFPDMQIISLSGNFCSDKKPAAINWIKGRGKKVVTECIISATTLRQVLKTDAKTLVECNKLKNMTGSAMAGSIGGXTPGSVIVVSWILPKDRQHKRRKEDNINTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFK2</accession>
    <name>T1GFK2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine-rich PDZ-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cysteine-rich interactor of PDZ three</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRIPT family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFK2"/>
    <dbReference type="STRING" id="36166.T1GFK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002146-RA">
        <property type="protein sequence ID" value="MESCA002146-PA"/>
        <property type="gene ID" value="MESCA002146"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133934_0_0_1"/>
    <dbReference type="OMA" id="MPCDKCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019367">
        <property type="entry name" value="PDZ-binding_CRIPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805:SF1">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10235">
        <property type="entry name" value="Cript"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002146-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11075" checksum="81ADC02410ACD39C" modified="2013-10-16" version="1">MVCAKCEAKLSKIVTPDPWKAGARNTTQAGGAGRKVNENKALTSYKDKANPVGTSFKPCRICRSKVHQAGSHYCQSCAYKKGICAMCGKKLLDTKNYRQSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFM5</accession>
    <name>T1GFM5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFM5"/>
    <dbReference type="STRING" id="36166.T1GFM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002169-RA">
        <property type="protein sequence ID" value="MESCA002169-PA"/>
        <property type="gene ID" value="MESCA002169"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2040734_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047134">
        <property type="entry name" value="RNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041:SF78">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00282">
        <property type="entry name" value="KAZAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002169-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="14169" checksum="716D7B6A77BB12DE" modified="2013-10-16" version="1">MECVICAEDLLTNGSVVTIPCGHIFHKTCLMKSLTYRSSCPLCRNRTFVTQLHTIYLNSILREIPAKICEEIREYKFEKRSPEIPEEYCFKTDTSIIKYIILFLGFFGIKNQSGLRFVWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGM1</accession>
    <name>T1GGM1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GSKIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSKIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002544-RA">
        <property type="protein sequence ID" value="MESCA002544-PA"/>
        <property type="gene ID" value="MESCA002544"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973025_0_0_1"/>
    <dbReference type="OMA" id="FAVTEMH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019207">
        <property type="term" value="F:kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037395">
        <property type="entry name" value="GSKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12490">
        <property type="entry name" value="GSK3B-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12490:SF4">
        <property type="entry name" value="GSK3B-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GSKIP" evidence="2">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002544-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14395" checksum="57C964C27D5CF089" modified="2013-10-16" version="1">MGNPSIPLRGRNPYDSDEELLTWEDEAAAVIRDIKNHVKEIFVSQSLPSNSFEIFINLETFENSKYCIRSSGSGFQIAGQGYDNKSIYESVMESEDQEPEIFETPYSLLSSISPGYNILLLRNYNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGQ0</accession>
    <name>T1GGQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NR LBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGQ0"/>
    <dbReference type="STRING" id="36166.T1GGQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002574-RA">
        <property type="protein sequence ID" value="MESCA002574-PA"/>
        <property type="gene ID" value="MESCA002574"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1429538_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035100">
        <property type="term" value="F:ecdysone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035076">
        <property type="term" value="P:ecdysone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003069">
        <property type="entry name" value="Ecdystd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF507">
        <property type="entry name" value="BILE ACID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01283">
        <property type="entry name" value="ECDYSTEROIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002574-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21037" checksum="08D37EB3CF16701B" modified="2013-10-16" version="1">MAGMADNIEDLLHFCRQMYSMTVDNVEYALLTAIVIFSDRPNLEKADLVEAIQSYYIETLRVYILNRHKGEPKCLVLFAKLLSILTELRTLGNQNAEMCFSLKLKNRKIPRFLEEIWDVPDPSRTDDSAALPAPQTPQGPHLMANGRPSGLPNETGPTLQVVGKWEADNEAMMMAVGGQSVGNHGHGGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHD5</accession>
    <name>T1GHD5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002830-RA">
        <property type="protein sequence ID" value="MESCA002830-PA"/>
        <property type="gene ID" value="MESCA002830"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963436_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002830-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6721" checksum="8A11CC09C89446CC" modified="2013-10-16" version="1">MLDLGVNLKYLGNVLVLKLQYHLNFGENPSKKDQIFSHCSNLTPSEKFIIKRGKKFVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHD6</accession>
    <name>T1GHD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02122424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02122425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHD6"/>
    <dbReference type="STRING" id="36166.T1GHD6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002831-RA">
        <property type="protein sequence ID" value="MESCA002831-PA"/>
        <property type="gene ID" value="MESCA002831"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443364_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF17">
        <property type="entry name" value="PROTEIN SIMILAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002831-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10664" checksum="4AD429BD9BFC88E7" modified="2013-10-16" version="1">MDFPNIDVLGNQIWDYCHQCDHDELKEALNIKKIKSLSSTQIKEEIEEVDELKTAHRDLFIRLKCTLTSRGRSVNIKSASREFLKFQKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHE0</accession>
    <name>T1GHE0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibrous sheath CABYR-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHE0"/>
    <dbReference type="STRING" id="36166.T1GHE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002836-RA">
        <property type="protein sequence ID" value="MESCA002836-PA"/>
        <property type="gene ID" value="MESCA002836"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080810_0_0_1"/>
    <dbReference type="OMA" id="DPACIFI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004011">
        <property type="entry name" value="Gyr_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02756">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00713">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002836-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23207" checksum="8E21A1DA56C395B5" modified="2013-10-16" version="1">MQEGIGNSWGLSKDPWGGGPPAEESAPIEAEPEQDSAVLADDGYQYKTVRRLKYRNRRDVSELSGYQYETPEVKEVPSPAPEYLPPVKESAPVEDEPEQDTAVLADDGYQYKTVRRLKYRQRRDVSEIAAAAPEYLPPAEESAPLEAEPEQDSAVLADDGYQYKTVPEEYLPPQQEAAEPEQDSAILADDGYQYKQIKKLKYRHRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHH2</accession>
    <name>T1GHH2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02061779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHH2"/>
    <dbReference type="STRING" id="36166.T1GHH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002873-RA">
        <property type="protein sequence ID" value="MESCA002873-PA"/>
        <property type="gene ID" value="MESCA002873"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_317911_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF23">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE USP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="677"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="249"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="390"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="495"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="567"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="495"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="530"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="577"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="596"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="614"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="653"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002873-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="916" mass="99736" checksum="679B779F3B4964FF" modified="2013-10-16" version="1">MPTSTTGSSNLFLQPTSATNGVSDISSKYSKFDKSNGYTSGLSSSLNSRYSSSSYKPSTSSSSSVSGSRYRSGSLSGGSISALSSYDSAPSSALSSSRFGRFKHHSHYDYTLPKRTSSFSGPGLNNYDTTTNYTTSTLRSRRNSLTRRPPSLYGGCSSNLSPHSSHSSVVNVDSIVNKSNRNSIANNQLPPLPSASSSSSSKSSALRSSSSLARYVATSGYDMIKQYSPAHYRPNSEPTLLSKSLSEANVNNNSNLNNNHINNNSNHLSSNNTQRERNVESIKGAIKQQQQKHFTSKLTSTNSLRNGNNGTTVTSTLNGTSSKLKDEFLKTEYELLVASKILEFENIASSSATDLKNNNGYHHHHQQHLQQRRTNMNCLDDIKFIDSDEFAGPIPTRPKTSVPTKSSPSTLPLVNKKSGIGSGSVVGTSSVDANSIKASINKFNTLSEMKRIPTRSSTGNLGSSNHHSRSHSHHHHHHNYIINNNISNDNNNYCGSSGSSSCSEKNYKSHSASPSSGTGSGSGSVTNALKKQSSASSPQSSRKASASSIFSTNATNLLKSTVICSASTKQPQHPQQSAATATTASSSSSSLSPKISDPKRDTSSKLSDNDDSIRTTSIRTTLPPSSPTSSRYLDNSASTSSSYKPSRKSTISESENGTTTNGKSANGDSETTDEGLCGLRNIGNTCFMNSVIQCLSHTNELTKFLKSQTYIKNTTKDQQILHEFAKLIREMWTPNIGSVTPHELKSAFSSKHRMYSGYNQQDSQEFLRFFLDSLHCALNSGTKGEHLKIDDSWSDNRIADLTWEWYSKVESSIVKDLFVGQLKSTLKCTVCGNTSVTFDPFWDLSVPLPSSSRCKLDSCLDLFIREEILDGDEKPTCSKCQTRRKCTKTFTIQRFPKYLVIHLKRFSETRGVNYQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHQ0</accession>
    <name>T1GHQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHQ0"/>
    <dbReference type="STRING" id="36166.T1GHQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002958-RA">
        <property type="protein sequence ID" value="MESCA002958-PA"/>
        <property type="gene ID" value="MESCA002958"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2365582_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF14">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="6">
        <location>
            <begin position="20"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002958-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10373" checksum="F3C726AC6669F789" modified="2013-10-16" version="1">MVQLYVPSEHHRPKGIMLRVGAYVSPQMMEGEYSMREKLPANVYTWTSRDPCIDGGRAVTVCAPGAAITSVPEFTMSKSMLMNGTSMAAPHVAGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHQ5</accession>
    <name>T1GHQ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002963-RA">
        <property type="protein sequence ID" value="MESCA002963-PA"/>
        <property type="gene ID" value="MESCA002963"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657323_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576951" evidence="1">
        <location>
            <begin position="28"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002963-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8615" checksum="A686E2DDAF4AAEDA" modified="2013-10-16" version="1">MVRVNVFNSLYASLCLLIILVVVSSYAAPMPSGDNTAYYNEVEETPEDLLMELLARSTELSTKREETLKRFKNNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIN1</accession>
    <name>T1GIN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIN1"/>
    <dbReference type="STRING" id="36166.T1GIN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003314-RA">
        <property type="protein sequence ID" value="MESCA003314-PA"/>
        <property type="gene ID" value="MESCA003314"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1549395_0_0_1"/>
    <dbReference type="OMA" id="IWISAAE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003314-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19076" checksum="3BEBDA741FD47A59" modified="2013-10-16" version="1">MLAGLIDANEKKMDADKSTQNFPITLAFKTFFWRILNCSLCWFTNVLVYYGLSLNSVALGGNKYDNFIWISAAEIPGFFSPVLLMDRIGRRYSLFGAMIVSGLSIGCSVFVPPAPMTPLLGVYYAEGPAIIFSICAIFSGFLALSFKETTEVELPNTLDDAEVIGNKKDERQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIQ1</accession>
    <name>T1GIQ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIQ1"/>
    <dbReference type="STRING" id="36166.T1GIQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003336-RA">
        <property type="protein sequence ID" value="MESCA003336-PA"/>
        <property type="gene ID" value="MESCA003336"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1350270_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021743">
        <property type="entry name" value="KRTAP_type8/19/20/21/22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11759">
        <property type="entry name" value="KRTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576963" evidence="2">
        <location>
            <begin position="21"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003336-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="20830" checksum="EB76D4B9DDC85B80" modified="2013-10-16" version="1">MKFLLSFVIAVLAVAAPTSATFDLLNALKSEGVGSYGNGYGYSGYGGYSNGYGYGGYNNGADVKVVKVYSYPSSGYSSGYGYSNYGQPAYGAGYARSSAVISTPVVSTPVVSTPVVYSSVVSTPVIKTAVATPLVSTYQAGYGGLGAGYGSGYNYGIGSGYGLGSGYGSGRINQGVILDSKLKWDKHLGSDAKNCKMVKLFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ13</accession>
    <name>T1GJ13_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">lysoplasmalogenase</fullName>
            <ecNumber evidence="6">3.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 2,3-saturated aldehyde + sn-glycerol 3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:22544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = a 2,3-saturated aldehyde + sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:16905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.2" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02134474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ13"/>
    <dbReference type="STRING" id="36166.T1GJ13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003452-RA">
        <property type="protein sequence ID" value="MESCA003452-PA"/>
        <property type="gene ID" value="MESCA003452"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1350269_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047408">
        <property type="term" value="F:alkenylglycerophosphocholine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="TMEM86B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="GH04784P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885:SF6">
        <property type="entry name" value="GH04784P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="22871" checksum="3B5276E518367787" modified="2013-10-16" version="1">MLFILMHKLQKNCYLQKILLGLIFSVCGDALLNIDLFPHGMGAFALAQICYISAYGLKPLKPLFAIPFYALAVALYVLVLKNLDPILTIGLPVYAFLLTTMVWRSMVQAHTTPDGLNKLCAFGSFMFAISDGFIAVDKFYVEIPHARVSILGGFCLKLELFNERMGGGLAIFPKRIHPAQANEKSKRFWKRKAVFYRGMCFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJC8</accession>
    <name>T1GJC8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003574-RA">
        <property type="protein sequence ID" value="MESCA003574-PA"/>
        <property type="gene ID" value="MESCA003574"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2694939_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003574-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8231" checksum="9F9C6E154A3337C8" modified="2013-10-16" version="1">MTDESSDINSEKHVNSGSSDESDADPDPMTRLTGLLRRATLQGNETKRIIEDLTLEGLAKHWKTNGFKNIITMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJF6</accession>
    <name>T1GJF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GJF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003602-RA">
        <property type="protein sequence ID" value="MESCA003602-PA"/>
        <property type="gene ID" value="MESCA003602"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1257335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="48"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003602-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="24047" checksum="699305D8165A3C4B" modified="2013-10-16" version="1">MPPADPDIAKMIGTRHFPWAGHVERMDDNAAAAKINNDSVEGVRRTAFDEEIISPLVYKVETVGMVYMAVSGAPDINPLHAEHAADLALRIIKKVKALNLPDLAIKIGIHSGPVVAGVVGLKVPRYCLFGDTVNTASRMESSGEPFKIQVSSYTAKKLKAKGYKLESRGHVKVKGKGDMETFWLISTPDEXXXXXXXXXXXXXXXXXXXXXXXXXXXYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJH0</accession>
    <name>T1GJH0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192311">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJH0"/>
    <dbReference type="STRING" id="36166.T1GJH0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003616-RA">
        <property type="protein sequence ID" value="MESCA003616-PA"/>
        <property type="gene ID" value="MESCA003616"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_403503_0_0_1"/>
    <dbReference type="OMA" id="CEANANK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF70">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 2, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="532"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003616-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="682" mass="80230" checksum="4C6FBD19CCCDDCAB" modified="2013-10-16" version="1">MTILEVNIGTLMSAYATHSTHSIELFPYNENRTKIKRTLFSIHRSVSLIMSLWTQHYKDLMFIDVNFFTKKGRPFELFEFTNTCCQFIDKTRSMLLEKWYGEIQDILIRGNKRRLLPDVRKPKMLGRFYNCVAALMTQQLEDLCIKSIEGYVNFMYDYGNVEDIFYHIIEKHYQKVMPEISLSLNSSPELRTLSHEDLTRNHPSKLPTNSRLYLFLTYDQIRKAKRVPWKSFCEENCNGASKLRKVLSKGKKSNPGQKISINLKDQDTIKSNPGLRISINLEDQDTIVFNPSFSKLRKELVRLIDTIVSGIDKFIRIENILYTDLKLSNDTYLKPAIPISIIQGAKEKIIFLLEEQRIGPELRLQDFDDYISLMNGIDAERIGKFIDSDPPFEDYCENLLLFKEKEAQIPKDLWGTYSTNIVDENDCRPTGRITRLGKEYETIAKKALSIPADTAELMKSKAYVALVEDKIAPEMEIRLRVNMDHILWLMDHAIYSPLEIKKNSNTFQWYLQLPQIFKENREIIADKTIEYQELLKRRIEQFRRDLDSYNEQVQEYNQWGDFSLLSKYKKKATILDNRLVAAMNTIDKINEEETAYGWELTQYPIRKKTHDQLKPFKTLFDAAQDFVDKHNLWMNSQVGTYEPEDIENDVSQIYRIVQKLERQMGDHPQTMILIRDMQYPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJS0</accession>
    <name>T1GJS0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02141406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003723-RA">
        <property type="protein sequence ID" value="MESCA003723-PA"/>
        <property type="gene ID" value="MESCA003723"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3261078_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003723-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="42" mass="4817" checksum="DA9C198EF234FFF5" modified="2013-10-16" version="1">MNLVDITTLPRIPQTQYLRNSPPKSSRKILWVIFGSICENGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKG7</accession>
    <name>T1GKG7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003990-RA">
        <property type="protein sequence ID" value="MESCA003990-PA"/>
        <property type="gene ID" value="MESCA003990"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349077_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003990-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11562" checksum="82BDE03090C02EC6" modified="2013-10-16" version="1">MRVNWKKKCSDFTGVVMSLLSARLCSILGNQERKRTKEWRNTEFDATLEEYIFRRRKNETYVDNNKNMETLMILGNKRGRKRSIKSGAPVLFLQFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKQ7</accession>
    <name>T1GKQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004084-RA">
        <property type="protein sequence ID" value="MESCA004084-PA"/>
        <property type="gene ID" value="MESCA004084"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729568_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004084-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8534" checksum="EFF620CED8B07B02" modified="2013-10-16" version="1">YLQYKVFFIKVNIQKNNTNIRHIKNDIYQTSKQMQSSTDFVTKSINLLAEVVDYVNYKKSPSLIRITNFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKX7</accession>
    <name>T1GKX7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKX7"/>
    <dbReference type="STRING" id="36166.T1GKX7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004160-RA">
        <property type="protein sequence ID" value="MESCA004160-PA"/>
        <property type="gene ID" value="MESCA004160"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_750715_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF369">
        <property type="entry name" value="FI18813P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="35"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004160-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="369" mass="41579" checksum="A3C279D9F07F23EE" modified="2013-10-16" version="1">MSLHFVVLSFDRYSRDDIIGEVVCPLNTINLEQIENQQVAISKEIQPRTSASRLTVVLLKARNLPRMDVTGLADPYVKIYLLYNGQRISKKKTHVKKRTLNPVFNESFAFDIPPNLNDSSLQGVSIELMLLDWDRVTKNEVIGKLELGGPNCTGSALNHWTEVCNSPRRQIADVVPSILGFSAAGVLLIIIYFCLNRLKERNQKTNHKLTGCNFKPNIKPKAVRSPNGQPPQHFKKSPSPTVPSKLLPGGESPQNIEQQQQQQQQPIPNLKTLKYTEENEKKRVESPDAEKVNINGNAEGHENENYGLLGTLVFKLRYFADGKAFVVSIVRCRGLPVKNQNNSEKLQASTDPYIKLQLLPDKQHKVKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL24</accession>
    <name>T1GL24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004210-RA">
        <property type="protein sequence ID" value="MESCA004210-PA"/>
        <property type="gene ID" value="MESCA004210"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1251918_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF56">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="221" mass="26253" checksum="B7F6F4DA9EE1395A" modified="2013-10-16" version="1">MRLVLTKLAYLHASSAVHENRNPGSFLKFKKNHHIHLDNLYKKVYKSLEKNLPSFTKLNLKEPELNENHFNCLVHGSVWEPNILFKLQNNSEDELKDVIFINYHYAYYGSPTIDLQKYIHSIMLENCNEAEKDLVEFYYYKLKDLLQRMVYKDKIPKFEEFWMQYNHNRVFGLQQILLINPFVISGKLQSLDVMKGIPSDDLCDEVFKNQKVIKYLNSTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL36</accession>
    <name>T1GL36_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02200097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004223-RA">
        <property type="protein sequence ID" value="MESCA004223-PA"/>
        <property type="gene ID" value="MESCA004223"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500500_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004223-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="10139" checksum="8B534F5687D3DAF9" modified="2013-10-16" version="1">MTDIRSMRGVNIESDNYLELKIRCDKVKPPLKSSYDGPYLIMGKQEILKLKINENISWKFPGAINDCNFIQELWINDIVHYIQEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLZ7</accession>
    <name>T1GLZ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004564-RA">
        <property type="protein sequence ID" value="MESCA004564-PA"/>
        <property type="gene ID" value="MESCA004564"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815383_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004564-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7943" checksum="5C1BDD3002275D3B" modified="2013-10-16" version="1">MILENNMPRILNRKGIHCNRDGHEMDNEIKMEGTRGRGRQRTRWVDLVGRDAKELGIPNWRLQLMIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMH5</accession>
    <name>T1GMH5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMH5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004749-RA">
        <property type="protein sequence ID" value="MESCA004749-PA"/>
        <property type="gene ID" value="MESCA004749"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199962_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004749-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11939" checksum="5CE180232B8A5126" modified="2013-10-16" version="1">MKNGDESRMTPQQKPLVFNSEEVRSAIQKLKNNKSTGSDGIPAELLKSANHKFHRCLSLAANKLNDITKASLRLVLLANVWKEFKKVSIPKVGKVGHSNAKDDIPTNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMN9</accession>
    <name>T1GMN9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMN9"/>
    <dbReference type="STRING" id="36166.T1GMN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004817-RA">
        <property type="protein sequence ID" value="MESCA004817-PA"/>
        <property type="gene ID" value="MESCA004817"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1350271_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004817-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="23244" checksum="4CAE0B981048362D" modified="2013-10-16" version="1">MKTMHQEREALKKLSNVKKDHAKRLDELTKNQEVDKQKAELITRNQELVDNAILAVRSALANQMSWPDIKHLVKSAQNKGDPVADSIKQLKFEINNISLFLKDPYRIDDSDDEDEEVELPSMTVDIDLDLSAWANARKYYDLKRVNLCLFSTWTKEVDNEQIPAVKNPNVLGDAFVNLQAFAKHLANVCNSLPLRLILFLEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMP9</accession>
    <name>T1GMP9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02062174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMP9"/>
    <dbReference type="STRING" id="36166.T1GMP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004827-RA">
        <property type="protein sequence ID" value="MESCA004827-PA"/>
        <property type="gene ID" value="MESCA004827"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111707_0_0_1"/>
    <dbReference type="OMA" id="FECMALK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004827-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17747" checksum="F074CB00E9FEF214" modified="2013-10-16" version="1">MAVRACIVEGKGECSPDKLKKHRSQMETLWKEISALADFDNDGKISAEEFKDAVNRTCVGKKYDQFPEPFKAFVESSFNLLDINSDGIVGLEEYRYNCISRIAIDDVGPIDKAFESLLNDDDRKKGGLDITRYKELYAQFLGNKDDKHQAVNLFGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN24</accession>
    <name>T1GN24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 216</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02191269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN24"/>
    <dbReference type="STRING" id="36166.T1GN24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004963-RA">
        <property type="protein sequence ID" value="MESCA004963-PA"/>
        <property type="gene ID" value="MESCA004963"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135948_1_0_1"/>
    <dbReference type="OMA" id="AEILMFV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019184">
        <property type="entry name" value="Uncharacterised_TM-17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531:SF0">
        <property type="entry name" value="GEO07735P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531">
        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09799">
        <property type="entry name" value="Transmemb_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004963-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16619" checksum="8B733AE518A3FADF" modified="2013-10-16" version="1">MPPKTELPKKSNPSLAYEVLIYLNSFYFGMFASCELGMGVLKAINLEYAQNPLIQDSAVLVSLCVLESLRCVMGRRGRLINKDWPVVTSIFLTIPCCMGVIYLLTLQTYKLRLEYCLCTLLLLLYLTELYYALAFVFSLCKPVTYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNC3</accession>
    <name>T1GNC3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNC3"/>
    <dbReference type="STRING" id="36166.T1GNC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005070-RA">
        <property type="protein sequence ID" value="MESCA005070-PA"/>
        <property type="gene ID" value="MESCA005070"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1215982_0_0_1"/>
    <dbReference type="OMA" id="FTVITIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051384">
        <property type="entry name" value="Mth_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47154">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47154:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005070-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="26882" checksum="26C12A3298151A00" modified="2013-10-16" version="1">MLQKQQQIVSSERSSLKSHQRKRFLLYNFYGWGLPILFTVITICLTNFDVLSDSLRPNFGQGRCWFQHDLSAAFFLGPIGVLILANLVFFVMTLKYCNKVKREIHRMQSSNEQKKVLRGNFFVDKAQFMMNTKLFVVMGITWVLELLSAIFYNRGRIFFWIVSDSFNVLLGVLVFLIFVFKKRVWQEVLQKLGEYFKENLLAEDMLEISSEEEEGHRKKEAYTEELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNG0</accession>
    <name>T1GNG0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02117150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02117151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005110-RA">
        <property type="protein sequence ID" value="MESCA005110-PA"/>
        <property type="gene ID" value="MESCA005110"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657308_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005110-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8886" checksum="0FF8F04F10FB77DB" modified="2013-10-16" version="1">MGLHDQLVKYLSLMNYSFKKYNERRILTPSIFVDVFKILVELEDAAGVILYQKFNQNSVPIYGANGEFRVKVRLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNG9</accession>
    <name>T1GNG9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 8">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 8"/>
    <dbReference type="EMBL" id="CAQQ02146590">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNG9"/>
    <dbReference type="STRING" id="36166.T1GNG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005120-RA">
        <property type="protein sequence ID" value="MESCA005120-PA"/>
        <property type="gene ID" value="MESCA005120"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039326_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00326">
        <property type="entry name" value="alpha_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF265">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025092229" description="Carbonic anhydrase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005120-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="303" mass="33939" checksum="61D9E0392CEA9BD7" modified="2013-10-16" version="1">MFKFIICATLLLSSSFAWKHSSQDSWGEESPDCNGKQQSPINLIPADAQAQNAQNIQFNGYDIQIPGNSLILVNNGHSAQLNFNEQTSNLTITGGILGEEKYILEQIHFHWGGANGRGSEHTIDWKPYDLEAHLVHRNTKFESVAEAAGKENGLTVLGFLYEQRSGLLGYNSDIRAITDRLPIIQNEGNTARLTTRNFITGRNFPADKQTTYFNYAGSLTTPGCSENVNWIVFDRIQGIAADELRKFRDLKTSDGDALVDNWRKIRDLNDRNVTLIKTADNFKIIPPRLIVPQQLNSIRADSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNJ7</accession>
    <name>T1GNJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNJ7"/>
    <dbReference type="STRING" id="36166.T1GNJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005151-RA">
        <property type="protein sequence ID" value="MESCA005151-PA"/>
        <property type="gene ID" value="MESCA005151"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044522_0_0_1"/>
    <dbReference type="OMA" id="EWAPGTQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="33"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005151-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="318" mass="36589" checksum="FA001F1A1DE3DBA0" modified="2013-10-16" version="1">MNLMRSRKNIMLNSIYQKNPEEVTAGGRDGSAYVLRCLKMWYELPSDVLFAAINLVDRFLGKMSVKPKHMACMSVASFHLAIKQLDYKPIPSEDLVTISQCGCTSGDLERMAGVIANKLGVQMGHAPITSLSFLRIYYGLFRNMTKELGGNLLDFYEKFIKLEELEVRLEVLLCDVQTTAVTSSTLALVLICLHLDFHIKESYPFKEAEGVELKHLFEYILYLQQLSRIHKRIQHCIRPLSHYNGQNKQPYKQRLVWKLSSRTLRILRPTNRFSTDLTTIEEGYPKKEDALRSRSESVSSEEEEDWPTSPIIPIFEQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNT3</accession>
    <name>T1GNT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02198078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNT3"/>
    <dbReference type="STRING" id="36166.T1GNT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005243-RA">
        <property type="protein sequence ID" value="MESCA005243-PA"/>
        <property type="gene ID" value="MESCA005243"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005507_3_1_1"/>
    <dbReference type="OMA" id="MPANAYL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF374">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="16">
        <location>
            <begin position="590"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005243-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="641" mass="72421" checksum="3EB0CD53AB861561" modified="2013-10-16" version="1">MSMDVSGGKIIWAKHSEMQQVNLKTLSDDAEVKDGERLPVAVKDMGACEIYPQTISHNPNGRFVVVCGDGEYIIYTSMALRNKAFGSAQEFVWAPDNNEYAIRENNGTVKLFRNFKERKSFTPEYGAENIFAGYFLGVKTSSGLAFYDWETLQLVRRIEVQPRNVYWNESGSLVCLATDDSYYILSVDTGAIVQVLESKQPIEDDGIESAFDVVGEITEQIRTGIWVGDCFIYTNSVNRINYYVGGEIVTISHLDRTMYLLGYVPKDNRIYLGDKELNTRFPKSRCSFTNNPSRTSHQGAHFLEKQGFKTQALQVSTDPDHRFDLALQIGNLDVALKLARESENPQKWSQLADIATKKNNMELVKECMQKANDLSGLLLLATSSGDDNMMKDLSEMGHENGKHNIAFLATFLRGDVEGCLKILIETNRLPEAAFFARTYIPSKMTETINLWREELAKHNEKAGQSLADPEKYENLFPGYTEALRLEKHLEKERNPNAKIPANLYSSFPLNIDRNPFEEMRQAPENLSDLLNTSSTVEDHKKNDDFLKAEIQTATSLDDELDLEIDGITLDDNIDTADVNLDDDDLDLKRCYLPLETGYCKASMPRFGYNFKQRRCVSFVYGGCGGNSNRFLTFQACLAACR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNZ1</accession>
    <name>T1GNZ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNZ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005304-RA">
        <property type="protein sequence ID" value="MESCA005304-PA"/>
        <property type="gene ID" value="MESCA005304"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834084_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005304-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7527" checksum="2C014F3A88EFF0F0" modified="2013-10-16" version="1">MNITFEGHKFLVIVVTVCLVQTVGRKLVLSDLSKLLERRWFHCMTQLPNFYSATYDSSAINVGDYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP14</accession>
    <name>T1GP14_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD repeat-containing protein 55 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat EMAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP14"/>
    <dbReference type="STRING" id="36166.T1GP14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005329-RA">
        <property type="protein sequence ID" value="MESCA005329-PA"/>
        <property type="gene ID" value="MESCA005329"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011754_2_0_1"/>
    <dbReference type="OMA" id="LHPSREM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000477">
        <property type="entry name" value="Echinoderm microtubule-associated protein-like CG42247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005108">
        <property type="entry name" value="HELP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF55">
        <property type="entry name" value="ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE CG42247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03451">
        <property type="entry name" value="HELP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like first beta-propeller" evidence="6">
        <location>
            <begin position="38"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="328"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="7">
        <location>
            <begin position="335"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005329-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60630" checksum="E98AA6F089C8F7C2" modified="2013-10-16" version="1">HGYRGIDARRNLWVLPSGELLYYVAAVAILLDRDEEAQRHYTGHTEDIMCMDVHPSRELVASGQRAGRDRKSQAHVRIWSTETLQTLYVFGMGELDGGVSAVAFSQLNGGSYILAVDAGRESILSVWQWQWGHLLGKVATLQEGLSGATFHLLDDNLIITHGRGHLAFWHRRKDGFFERTDIIKQPSRTHVTSVQFEADGDVITGDSDGFITIYSVDSDGAYFVRTEFEAHNKGIGCLIMLSEGTLLSGGEKDRKIAAWDSLQNYKRIADTKVISRIAMKCNSLPESAGGVRTIYPQRPGRNDGNIYVGTTRNNILEGSLQRRFNQVVFGHGRQLWGLASHPEDDLYATAGHDKHIALWRKHKLLWSIQTGYTAGSTEGHLLIISAENGSVMLTLRVCGSPINCISFNQVGDMIAMGSQNGSIYLFRVSRDGFSYKKVNKIRGSQPLTHIDWSVDGNFLQTVTIDFDLLFWDAKSLSPERSPIAMKDVKWLTHNCTVGFMVAGMWSNRYYSNSAAIISTCNRSTTQEYLISGDAEGYLRLFRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP93</accession>
    <name>T1GP93_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02135088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP93"/>
    <dbReference type="STRING" id="36166.T1GP93"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005414-RA">
        <property type="protein sequence ID" value="MESCA005414-PA"/>
        <property type="gene ID" value="MESCA005414"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838557_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005414-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7248" checksum="AB9145CA449EFFB7" modified="2013-10-16" version="1">MHALCLPLGASVSLLIMFFFFDSMQLLFAVCTAIIATVALAFLLLPMCQYIIRPCTDGNRISFGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPS9</accession>
    <name>T1GPS9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPS9"/>
    <dbReference type="STRING" id="36166.T1GPS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005622-RA">
        <property type="protein sequence ID" value="MESCA005622-PA"/>
        <property type="gene ID" value="MESCA005622"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1002171_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12322">
        <property type="entry name" value="RRM2_TDP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000107">
        <property type="entry name" value="TAR DNA-binding protein 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF9">
        <property type="entry name" value="TAR DNA-BINDING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="59"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005622-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="31179" checksum="59A130D039C73AC5" modified="2013-10-16" version="1">MAQIKKDIKSGQSKGFGFIRFSSYEVQMNVLATRHLIDGRWCEVKVPNSKQGTAQQVPCKVFVGRCTEDLSSNDLKEYFSKYGEVTDVFIPRPFRAFSFITFFDPEVAQSLCGEDHIVKGVSVHVSNAAPKAENSNRHSSRSQQIQHQSQPQQIPIQQMRSTPNYQRIRSKTIPWQETHNQHNSQEHLQMPSQLDLPNLQALGIGAGERSQPQQQAQNPLSVGLNFNANRSIMSGLNQWSFLSSGGQPSKNNGFFNWMNPPISSKDGNPSVVETTTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQJ6</accession>
    <name>T1GQJ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large proline-rich protein BAG6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">BCL2-associated athanogene 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">HLA-B-associated transcript 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).</text>
    </comment>
    <comment type="function">
        <text evidence="1">Released extracellularly via exosomes, it is a ligand of the natural killer/NK cells receptor NCR3 and stimulates NK cells cytotoxicity. It may thereby trigger NK cells cytotoxicity against neighboring tumor cells and immature myeloid dendritic cells (DC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of the BAG6/BAT3 complex, also named BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35. Interacts with GET4; the interaction is direct and localizes BAG6 in the cytosol. Interacts with UBL4A; the interaction is direct and required for UBL4A protein stability. Interacts with AIFM1. Interacts with HSPA2. Interacts with CTCFL. Interacts with p300/EP300. Interacts (via ubiquitin-like domain) with RNF126; required for BAG6-dependent ubiquitination of proteins mislocalized to the cytosol. Interacts (via ubiquitin-like domain) with SGTA; SGTA competes with RNF126 by binding the same region of BAG6, thereby promoting deubiquitination of BAG6-target proteins and rescuing them from degradation. Interacts with ricin A chain. Interacts with VCP and AMFR; both form the VCP/p97-AMFR/gp78 complex. Interacts with SYVN1. Interacts with USP13; the interaction is direct and may mediate UBL4A deubiquitination. Interacts with ZFAND2B. Interacts with KPNA2. Interacts with UBQLN4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02029342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029346">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQJ6"/>
    <dbReference type="STRING" id="36166.T1GQJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005905-RA">
        <property type="protein sequence ID" value="MESCA005905-PA"/>
        <property type="gene ID" value="MESCA005905"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1054821_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000161">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021925">
        <property type="entry name" value="BAG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204:SF0">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12057">
        <property type="entry name" value="BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="24">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="141"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00046003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005905-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="264" mass="29183" checksum="8046C4060D73CD20" modified="2013-10-16" version="1">MIIQLKVKTLDSRLYDFELDDEITILQFKDLIAEKTNIAANMQRIIYCGRVLLDDKKLKEYDVNGKVVHVVERLPPSTRPQSTSTNTNTSSTSGTGQRRENNPPFLRTLDGMSIAEALEGIFTNALRGQNVQNANVTLVELPVPPSSENNTANNSETTNNTDEPNTTQVDGEESSSSSTAAVAGSEEGVPNANEAGNDEPNVEEDPTDGTNGEGQRLRQRTRTQVLARVVEQMRNVQTRLAPFIDQYYEILSSDPNFEESIFAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRJ7</accession>
    <name>T1GRJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoplasmin core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRJ7"/>
    <dbReference type="STRING" id="36166.T1GRJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006282-RA">
        <property type="protein sequence ID" value="MESCA006282-PA"/>
        <property type="gene ID" value="MESCA006282"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856317_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024057">
        <property type="entry name" value="Nucleoplasmin_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036824">
        <property type="entry name" value="Nucleoplasmin_core_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03066">
        <property type="entry name" value="Nucleoplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69203">
        <property type="entry name" value="Nucleoplasmin-like core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin core" evidence="1">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006282-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7150" checksum="C5EAB019494A8446" modified="2013-10-16" version="1">SLIITLVHTVKDSLKIPIAVLKAGETRAVNPDVEFYDTSVTFKLIKGNGPVYIHGQNLKDESEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSR6</accession>
    <name>T1GSR6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02028878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSR6"/>
    <dbReference type="STRING" id="36166.T1GSR6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006727-RA">
        <property type="protein sequence ID" value="MESCA006727-PA"/>
        <property type="gene ID" value="MESCA006727"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1338922_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09923">
        <property type="entry name" value="SH2_SOCS_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF32">
        <property type="entry name" value="LP02169P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="120"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006727-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="24232" checksum="3F7771528231F907" modified="2013-10-16" version="1">MNPANETMPRIRKWKGTSTCKRHGDQNMDLEGQALGNAGSTQDLKKIRRPKDVTTKNIRRGRLCSPSRNSHSKKRCCIHIFTGVFYPLYENYKDRRSFVKEFLGVVYHSLPDHESIIHVWYWGDISRKDAQKQLTDKPNGSFLVRDSETIDNQFTLSFRIGNHTLHYRLKYFENFWNFEDLKFDSIVEMIQDIIIEVRLKILFVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSW4</accession>
    <name>T1GSW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02011262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006776-RA">
        <property type="protein sequence ID" value="MESCA006776-PA"/>
        <property type="gene ID" value="MESCA006776"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3426992_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006776-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="21" mass="2324" checksum="10423D9B216C3F9B" modified="2013-10-16" version="1">MSLGTQSISNQLEIRGFTQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSX1</accession>
    <name>T1GSX1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006783-RA">
        <property type="protein sequence ID" value="MESCA006783-PA"/>
        <property type="gene ID" value="MESCA006783"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349066_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006783-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11423" checksum="C585BDE16B81F8DF" modified="2013-10-16" version="1">MYQLSPILDPGKLPRLSGWRDEECDKAVAEKDPLYRKNGRGNTRPTKKVFKNRRSEIVKSARQQKRRFEKAQLEILEYIGIMQTNSTIMYTVSEKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT17</accession>
    <name>T1GT17_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT17"/>
    <dbReference type="STRING" id="36166.T1GT17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006836-RA">
        <property type="protein sequence ID" value="MESCA006836-PA"/>
        <property type="gene ID" value="MESCA006836"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1760865_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="97"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006836-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="17157" checksum="D2B6D2E09A68BA1B" modified="2013-10-16" version="1">MEPLEKCSEFGSRSNHEKHPFPLSRTTKELPEILPFVFDCLYLLICAFKKRLIRISKDNFEVLRCFGSRFCVRFSTRIQDLNTAERLKRPKPLNYPPIAFVIFDCTEGEKDTCSKYGVSGYPTFKIFKPGEVSQDYTVQKKPKVSSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT31</accession>
    <name>T1GT31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT31"/>
    <dbReference type="STRING" id="36166.T1GT31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006851-RA">
        <property type="protein sequence ID" value="MESCA006851-PA"/>
        <property type="gene ID" value="MESCA006851"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_409719_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF1">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="171"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="621"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="634"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006851-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="671" mass="72294" checksum="0E28190B10430A6B" modified="2013-10-16" version="1">MSIEEFATPNSEVPPPPYSALYKRRTSPDDGEDSGLDENFHTPHKVRSLNNLVYEIEEELVKEEFERLKVENETFKVPRLHTPPKNGKFRKNKIGNAKFIGNDEDIITDLPDMKSFPIAKMKSLGTIPDIIAGERIEYEPFLTPLLARKIEQLQKHDEQESFDVVDGGPSTLTTNKWSMIKNFHGSFEHLRSGGSYITPGMTVDTPAFERGFRRSLQQRNTTLLVQNSTPTRQSDGEHVEDHRVLWNKNFEFMCKMSANASTGVLDPCVLRISIRKEIRGGRSYHKLGYIDLNLADPSPNLKSKQIPSQDDLGTVVGANQAINQGSVPPTTSSVTATSSTNALVGAGNRSTSSSRGTDECNIPQSQTSSGFGSLTKKKSDLYTEETVYILDSGLSESSDPTTNPSDSLPPLVTTNAQQLQQQQQVLPTGGLVGGVIASSIADCGHSRNSSNTSQKSLNFWLTEERSIVSHRGKSITGVPRRLLDGSTRQISSMTAAPSSSVVTSTISPNASCGTLGSINSTSSSGSSGAAGGNVTFFGTSDDSGENDDSITTAVLPVDEVDDDVAATTCCTTTSNTPLNTTPASLILSINSSGTIPGGVGENPSSGSLVISETGSLERMKNVPEKRKLGGALDDGGHRVSDRVEETRVNPESLIDDILKDTNPEDFERSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTF6</accession>
    <name>T1GTF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH dehydrogenase [ubiquinone] 1 subunit C2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I NDUFC2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02067388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTF6"/>
    <dbReference type="STRING" id="36166.T1GTF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006986-RA">
        <property type="protein sequence ID" value="MESCA006986-PA"/>
        <property type="gene ID" value="MESCA006986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160193_0_0_1"/>
    <dbReference type="OMA" id="PIWNPMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009423">
        <property type="entry name" value="NDUC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13099:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 SUBUNIT C2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13099">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B14.5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06374">
        <property type="entry name" value="NDUF_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017834">
        <property type="entry name" value="NADH-UbQ_OxRdtase_b14.5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12711" checksum="CB7B56292E229A9C" modified="2013-10-16" version="1">MNDPLELLENKNNRIPSFLSDKYNPVGCGLLGVGAATFINWALRRPLLSGVQKHIILGVGGVLLGSWLEEKRVAHFAEKDAVLRHYVQLHPDDFPVKPRVKFADVLESWTPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTS4</accession>
    <name>T1GTS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014355">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007115-RA">
        <property type="protein sequence ID" value="MESCA007115-PA"/>
        <property type="gene ID" value="MESCA007115"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294844_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007115-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11195" checksum="816865D32780AB5B" modified="2013-10-16" version="1">MIMKSARKKTNYRVSQVSTQETLPTQMATNYDRCPFVTRIATNILVRQETLSWSHHTNGAQISPGPSLHTNGAQISPGPTPLVAALQGWLLSVNYSERDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU11</accession>
    <name>T1GU11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU11"/>
    <dbReference type="STRING" id="36166.T1GU11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007210-RA">
        <property type="protein sequence ID" value="MESCA007210-PA"/>
        <property type="gene ID" value="MESCA007210"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266811_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577704" evidence="2">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11883" checksum="44F8104D2E4820DA" modified="2013-10-16" version="1">MSVCTVFVFMALMEYCLINIILNDTPIPKPIAYPPKPEPSKDPPQPKLDVKKEEQKIEKIFDEMTKNRRVGHQSFRQRSAMENSDGPWIPRTESRIILTPTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU12</accession>
    <name>T1GU12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Thiolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU12"/>
    <dbReference type="STRING" id="36166.T1GU12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007211-RA">
        <property type="protein sequence ID" value="MESCA007211-PA"/>
        <property type="gene ID" value="MESCA007211"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_1"/>
    <dbReference type="OMA" id="ICPSIAI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF107">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="8">
        <location>
            <begin position="225"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="5">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007211-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="36638" checksum="CB58503F187F44F4" modified="2013-10-16" version="1">MKASDLGSIVIKEVLKRANCSPDDVNEVVMGQALTANQGQNPARQASLQAGLSIDVPAYGLNLLCGSGLKSVCLGYQSIKAGDSSVHICGGQESMSQAPHVIHLRDGIKMGPGQIQDTMIKDGLTDAMKQIHMGITAENLKQAQKNGWFDQEIVPVEVTSRKGTVVFDKDEYIKPGSTVEQLAKLRPCFITDGSGSVTAGNASGINDSAAAVLLMSGEEVQKRGLKPLAKIVAWSQTGCNPEVMGIGPVKAVNEVLKKANWTKEEVDLYELNEAFAAQSLSVLKSLELDESKVNVNGGAIALGHPIGASGARILVTLLYLLQRTNSKKGVASLCIGGGMGIAMAVERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU55</accession>
    <name>T1GU55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HAUS augmin-like complex subunit 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU55"/>
    <dbReference type="STRING" id="36166.T1GU55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007263-RA">
        <property type="protein sequence ID" value="MESCA007263-PA"/>
        <property type="gene ID" value="MESCA007263"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559157_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032733">
        <property type="entry name" value="HAUS3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14932">
        <property type="entry name" value="HAUS-augmin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAUS augmin-like complex subunit 3 N-terminal" evidence="11">
        <location>
            <begin position="18"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="82"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007263-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="20435" checksum="22298A4F37F658C0" modified="2013-10-16" version="1">MPPKLNLFETLGFDDSCWMLYDENLQNFFNFLENNVTKENILTDEEIQISADWKSRNAPMLSEEECNEKLKEYSKKFEGVANENIDREIEAVELEILDLEQIKNSYDEVNQEMEQNLEFTKTKISALESKIIELETAEKQAHEKCCLWQGKSKMFKRRTRSCRIKLRICFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUA3</accession>
    <name>T1GUA3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUA3"/>
    <dbReference type="STRING" id="36166.T1GUA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007313-RA">
        <property type="protein sequence ID" value="MESCA007313-PA"/>
        <property type="gene ID" value="MESCA007313"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053503_0_0_1"/>
    <dbReference type="OMA" id="NTEYRAR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF187">
        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="1">
        <location>
            <begin position="185"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007313-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="46286" checksum="85799F3FDB982A35" modified="2013-10-16" version="1">VLKILTWDTVNHLVYYLGTQDKKPGQRHLYVVKDPTNDEGRKSEPQCITCDLGEALWSSRYYYVNCSHFDAFLSPSTTLIPDNGISHYILECQGPGLPVSGVHASKSHSLMKILYDTRPYYTERLQQLALPTQKSFEIPLPHGSRAQVQLLLPPSWREELRDAAFPVIVEVNGRPGSESVSEKFHIDWGTYMSSRNDVIYIRLDVRGAKGQSKKALYRHLGGAEVQDQISVLRNLFRIFSSILSIYLLETLSFLDETRVGIWGWGYGGYVTSMVMGSQQTVYKCGISVSPIADWLFYNSAFTERILGLPAENYKGYVEADATQRAKYIKSNSFFLVHGLADSTAPYVHGVQLARSLSDAGILYKYQTYADEGHDLSGVLEHVYGSMEQYFSECLSLDPDDTKPEDARVPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUE1</accession>
    <name>T1GUE1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02132914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUE1"/>
    <dbReference type="STRING" id="36166.T1GUE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007351-RA">
        <property type="protein sequence ID" value="MESCA007351-PA"/>
        <property type="gene ID" value="MESCA007351"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="OMA" id="WIITANH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577719" description="Peptidase S1 domain-containing protein" evidence="4">
        <location>
            <begin position="17"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="20"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007351-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="24109" checksum="09BEF8B7C8B6D1F7" modified="2013-10-16" version="1">MLFIVSVLFYLGATNAQERIFGGEAAVEGQFPYQAGLKTWDYNGRGSVCGGAIVNDHWILTAAHCIDGVSKWNLDQRRTMVYLPEPIELNNFVKPVELHLDSDESYVGENVNVAGWGMNSPEGIPPNLLYTQLKVQDPKVCQDFFPIPFDESQICVAATKTQSACPGDSGGPLVLADRPNYISIGIVSFGMDCFKGDPIGFARNSAQKKFIEETMNSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUG7</accession>
    <name>T1GUG7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUG7"/>
    <dbReference type="STRING" id="36166.T1GUG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007380-RA">
        <property type="protein sequence ID" value="MESCA007380-PA"/>
        <property type="gene ID" value="MESCA007380"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_866809_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000166">
        <property type="entry name" value="RIM-binding protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040325">
        <property type="entry name" value="RIMBP1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234">
        <property type="entry name" value="RIM BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234:SF19">
        <property type="entry name" value="RIM-BINDING PROTEIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="183"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007380-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="321" mass="33789" checksum="464D576014545FD3" modified="2013-10-16" version="1">MPMLSIHNTNLPSGSASYTLGLNSGSSSYQPPLGGIVDLTSLVSSTLGSGLTGPVGTAGTTGQLPSSVLYGPGLSGPSTYPTTYDLGGATLSSTSSYSNPYSNILTASKLKPIDDIDIGLGRFTRSSPCSPIIPNSWALDEFSEGLLHSGRTGLPLGSLDIDTRVGLNGMENQVDMLDIPGKGRCCVYIARFPYDPPEDAEGELTLCAGDYLLVWSSGPGGGEPLNGYLDGELLDGRRGLVPSTFLQRLIGEDLLEFHQAVLSTLRDPDEIAMSESTSIGLLPPIQPNNPLLTHTHEDLVRLSETQTDLDLENDEAADNDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUU9</accession>
    <name>T1GUU9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02061048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GUU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007520-RA">
        <property type="protein sequence ID" value="MESCA007520-PA"/>
        <property type="gene ID" value="MESCA007520"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_243853_0_0_1"/>
    <dbReference type="OMA" id="PQNIVIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="500"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="532"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="629"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1360"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="876"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="979"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1194"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1562"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="988"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1004"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1195"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1224"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1244"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1564"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1580"/>
            <end position="1612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007520-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1612" mass="178973" checksum="58D220FB96F2548B" modified="2013-10-16" version="1">MPKPNLKSCNSLYPIWENNELYFTEFEEQVEVESTVATTTADAFYVAAADVVNEPLCDEELKTGRNKRRSSLHRKPQQKLAKINIKLEKSPNQFCCAECGKSYKHPGNLSRHIRLECDVPARYNCFVCGREFRQLSNLRRHVATLHRRFKPPKTEKTDYGDCNGCTTMAAIHFANFLGSTILNSSSSSVAAASSPSVSAILPQTAAASSATTTLATTISTSQPSTPSSSSSNPLLITATKLLQQQKKKPNQFNCTNCSFRMVIGAAEIKSDRFPCQVCGKSYLRKRHLQRHMRDECIGIPPRFSCELCPSKFRRKYHMVRHIQSKHSGTPQALQLAAVKEELNATNLSTSNVENYENDMAAAAALASFSNGSANGGGSTSGSISGNDSPNMFNDFRTYAGTMAAALYPTSDSPGQAMMGGIVVKDEPISPGKAAQKMEDDWKMKLNFQCEKVKPERQLRRQRMPTSIASSSYYTPNTTPQDSSSLLASLQHTLQQDQPRYTCNLCGKSYSSRGTLGRHCRYECPFTGTQKKFHCPHCNFHSKRKDNLKCHIITHLKKKEKYTIQKRKIPIILLITGRMSSSSSSMFSSNNNNNNNNNNHSPSPQHLLRDFWYELKFSDLFKFINSDGRYQCPRSQCQKSYKDASSLQRHISESIWKISVKSVSTINHKTEERKPSSVSTLNTIGLFDQSTDLEQRHICEACGKSYSYRKNLSRHIRYECGVEPNIACPKCPYVTKYKSSIRIGNETFFTAKQDKGEQTDMNEGDFELDDCLLESNNIVITQNKDGYVLHVQKLGNVTEEAVVAAAQAATGSEHTQITSVQELKPHLATLQTSHAIKLPSSECELINIKKAPQPQPLQPTASSTPITTATIIHQPTIQHQPTTQHIKRPNRDQEPSTTHQVFRGNIITTQSGTTTTVMTTADLKDDGKHEKYEITEIDLNAQNSASIISDLVKYAEIDDIELPDGTKIGFGIGPQEITTHHHHHHHIDTSTQGSGVEHVQQSELQAHEHQEHDHELHQQEHLHHQQQSEQVSGVVVSDVSVHETHQAPTITKSYTILQSRPIKHEQSTTYELSLSDSSLTQGDESNEQKLYVCRHCGKKYRWKSTLRRHENVECGGKEPCHPCPYCSYKAKQRGNLGVTEWVWREFLKVFVGGNFGITRIAGLTWTEWNARLAMPLVTSLREGKHPLLFPTDLTIEKQSNSSSKSTIHEQSTSSSPDRKSPGFYSPNNSGNCIPSNALTSTPAQNSSSSSSNSNNNNNSSNTNNQKLNNSGSNNTSNSNNNTSPLDGACSDNEQDAGTFDSKNASGLCNINNINLLSSLSAMSSFMNSAAVGGGGAPSSAHHSLGSGGNTSMLGGANSVGHPCPDCGRLYKLKSSLRNHQKWECGKEPQFKCPFCVYRAKQKMHIGRHMERMHKEKFMKLDEKIFAAAAEEAGVHLPPVLCIFRAHILKYLDFLVSDAGSEGMEAGKSKSVATSTPKDNARIRVRNWFMMADKNILEKENALKQKALTTPSSTPRILHKPNILRKVNISNNNKQNATKQLRSSQVVLEDDVNLTDFVVKIEQNVSPSPSSSPSSLVMNLEAENENSNDDDVDMNGDDIEDEEKSDSDGLFDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUW3</accession>
    <name>T1GUW3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007535-RA">
        <property type="protein sequence ID" value="MESCA007535-PA"/>
        <property type="gene ID" value="MESCA007535"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978528_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007535-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="33397" checksum="895586FF55E73F1A" modified="2013-10-16" version="1">MHASNTRPKFIYSEHQFPSLVKEKHDDIPRPSQGLYSEQFKSNSKDDLFISNLIKIVHWNAQGISNISKITELNNLLDNLNIDIVLLNETFLKPNHKFYLNNFNILRSDRETMEVELQLVSTTNTPRTYNEIYSYKEANWINFKSFIDEELKHIIIHPNSVSIDTALHKFSSTILLARNKYIPKRQQINKSLEISSNTKLCISERNRIKRKLQRCQNSTEKNVLSSIVRQLNSLIRQNIFNDRNKSWSNMLSKLEPGDNKFWKITKFKRGKSNNNIGTLIVNNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUZ2</accession>
    <name>T1GUZ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUZ2"/>
    <dbReference type="STRING" id="36166.T1GUZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007565-RA">
        <property type="protein sequence ID" value="MESCA007565-PA"/>
        <property type="gene ID" value="MESCA007565"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1226186_0_0_1"/>
    <dbReference type="OMA" id="PSTWING"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007565-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="20522" checksum="43C7F63A9ECDBF3B" modified="2013-10-16" version="1">MSFPETYVDGFHDREAVIQMEYRSFGKTNMKVSKLAIGGATFSNLFGPLDEQEGIDTLHTALKNGINYIDTAPYYGDVNLKIGRYKATDYDTMFDYSAKKTRESIDLSLKLLGVDYIDVITIHDIEYVPTLDMILNEALPTLEKLKKEGKVRNIGVSAYPMGILNSQKNNSKNLKEITVTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVD5</accession>
    <name>T1GVD5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVD5"/>
    <dbReference type="STRING" id="36166.T1GVD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007731-RA">
        <property type="protein sequence ID" value="MESCA007731-PA"/>
        <property type="gene ID" value="MESCA007731"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1215985_0_0_1"/>
    <dbReference type="OMA" id="YEIDAAP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF37">
        <property type="entry name" value="LIM ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="54"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007731-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="27062" checksum="291254DB47B4EF0B" modified="2013-10-16" version="1">MEVVEENCTTVVTVEKIEKKKTKKSKSSKNSMKKSFSKFDALQKKPENEQPEQKICQVCEKPVYKMEEILIKKKISFHRNCFKCVECRKPLSADFFQSEDGTLYCSIHWKKKFEKRIDDEEKENGTTPLRKTELIIRESNPVPLPPDVVRSSEKSSLGLEDLEKRNNRNIRSRFENGWNEKMAKMEDDEEDDSEFENDENEDNENRRQPKFSQMSEFRRSSNMELKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVE2</accession>
    <name>T1GVE2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393892">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393893">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007739-RA">
        <property type="protein sequence ID" value="MESCA007739-PA"/>
        <property type="gene ID" value="MESCA007739"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906648_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007739-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7249" checksum="8C86D799DF3F0B42" modified="2013-10-16" version="1">MIQHFGPFQASLARKKKGSRNRVNVKFEIKELENIEIKGPKNDQETNKLLYKISLVHPFAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWN4</accession>
    <name>T1GWN4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GAIN-B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02124048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWN4"/>
    <dbReference type="STRING" id="36166.T1GWN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008212-RA">
        <property type="protein sequence ID" value="MESCA008212-PA"/>
        <property type="gene ID" value="MESCA008212"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2596958_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GAIN-B" evidence="6">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008212-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9295" checksum="343C573B9E8C3050" modified="2013-10-16" version="1">MWLDIDPSRYGHRSNPQCVRWNSFTNQWTRLGCQTEVPNYDEPIQEGDPIYINCTCTHISNYAVIVDIIDPEDIPEPSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWW4</accession>
    <name>T1GWW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008298-RA">
        <property type="protein sequence ID" value="MESCA008298-PA"/>
        <property type="gene ID" value="MESCA008298"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592511_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008298-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9280" checksum="D5CF122603C68421" modified="2013-10-16" version="1">MCFKKRLLIAGISVFLAVLLINFDIVEFINVCRINLSPRIIFLDPWRQPTVPVTTKINSCLDKMTSFMFKKSVQLFTKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWW9</accession>
    <name>T1GWW9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAM20 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWW9"/>
    <dbReference type="STRING" id="36166.T1GWW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008303-RA">
        <property type="protein sequence ID" value="MESCA008303-PA"/>
        <property type="gene ID" value="MESCA008303"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3052737_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF22">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN CG31145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM20 C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008303-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="54" mass="6096" checksum="C08D2B0C94926C55" modified="2013-10-16" version="1">MRKKMNHDPVAPVLWEPHLAALDRRIEIILQGVRDCLKKNPSDDNSAAEDDVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXE8</accession>
    <name>T1GXE8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neurotransmitter-gated ion-channel ligand-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXE8"/>
    <dbReference type="STRING" id="36166.T1GXE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008496-RA">
        <property type="protein sequence ID" value="MESCA008496-PA"/>
        <property type="gene ID" value="MESCA008496"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2365579_0_0_1"/>
    <dbReference type="OMA" id="IHNERRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008496-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11302" checksum="4FF8AB7882778DB4" modified="2013-10-16" version="1">GTLTVNVNVLLLSLASPDESSLKYEVEFLLNQQWNDPRLQYGNKSQYDYLNALHHHDNIWTPDTYFIMHGDFKDPIIPMHFALRIYRNGTITYAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXH4</accession>
    <name>T1GXH4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02053978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXH4"/>
    <dbReference type="STRING" id="36166.T1GXH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008525-RA">
        <property type="protein sequence ID" value="MESCA008525-PA"/>
        <property type="gene ID" value="MESCA008525"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349053_0_0_1"/>
    <dbReference type="OMA" id="EWKMIRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF45">
        <property type="entry name" value="CYTOCHROME P450 6G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008525-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11569" checksum="F2D78A76B7ACAD60" modified="2013-10-16" version="1">MVNKWYNHPAGKDQPFVGIHVFQKPGILVKDPELVKQVMIKDFNKFSNRFLAADFKNDPLAAFNLFFTKNPEWKMIRTKLSPVFTSGKIKQMFHLIDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXY4</accession>
    <name>T1GXY4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXY4"/>
    <dbReference type="STRING" id="36166.T1GXY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008697-RA">
        <property type="protein sequence ID" value="MESCA008697-PA"/>
        <property type="gene ID" value="MESCA008697"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402160_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008697-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10858" checksum="4AABB106C4B28ED4" modified="2013-10-16" version="1">MQETAWLVQNDFDFDKANSIPLTERSPFVEIEGLQDGICKSFKKSDDLPSPRLLKSHLPASFLPKEIWQKRSKIIYKLLVTLEVRRNNLLKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYE8</accession>
    <name>T1GYE8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02078426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYE8"/>
    <dbReference type="STRING" id="36166.T1GYE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008873-RA">
        <property type="protein sequence ID" value="MESCA008873-PA"/>
        <property type="gene ID" value="MESCA008873"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779554_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008873-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="16888" checksum="B726D5BD6BF0EE3E" modified="2013-10-16" version="1">MNTVATQANQREIRHLKMFYILISLMAAFIVLRLPAWIFLIMRMYGDYTQPVDWILHFVFGILTLASCVLNPLLYTFLTETIHYCEFTIEKILLKLTPVVLINSIDMPFILWIALSTLALVDGGTYFCFGTPGTDFFLEDILPFQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZN1</accession>
    <name>T1GZN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GZN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009335-RA">
        <property type="protein sequence ID" value="MESCA009335-PA"/>
        <property type="gene ID" value="MESCA009335"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066316_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="77"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="294" mass="33986" checksum="A7785B2FBF7332DF" modified="2013-10-16" version="1">MNKQEFQFRFLIPRQIFHGNLLDVTPNEKKTITENLRDKLQRGLFKFNVKPREIKGRKNKQASSLYLEETLLPIFGANLESLEKHFTFKNVPKFLVDAIDIIERIENIEVDGLYRISGNKVDVDSLKKHLMTSRYDKSCLYTEDINTLTSLIKLFFRELSTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRLPSNLLLPESIPEIRDILNRIPDLPKDTLKFLVKHLKIVSKHSSMNRMPASNLAIVWGPCIMAPNNLGPLDIARMNTLAKVMIESFDDIFKDNERYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZV5</accession>
    <name>T1GZV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endonuclease</fullName>
            <ecNumber evidence="10">3.1.30.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.-" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02386200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZV5"/>
    <dbReference type="STRING" id="36166.T1GZV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009413-RA">
        <property type="protein sequence ID" value="MESCA009413-PA"/>
        <property type="gene ID" value="MESCA009413"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055174_0_1_1"/>
    <dbReference type="OMA" id="LCNISPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018524">
        <property type="entry name" value="DNA/RNA_endonuclease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF5">
        <property type="entry name" value="ENDONUCLEASE G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01070">
        <property type="entry name" value="NUCLEASE_NON_SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="12">
        <location>
            <begin position="68"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="11">
        <location>
            <begin position="69"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009413-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="287" mass="33338" checksum="474E9F2320992653" modified="2013-10-16" version="1">LQSCYILYVFCGATAVRNSATRAATELFDTLSNSSHVSLNSSRVTNIPHRFVDILKKFQLPEIESVRYYSDYILYYDTRNKIAFWVYEHLTADSVRNSHVNRRYSEFLPEEDIHPFFRSNNGDYYRSGYDRGHLAAAANHKLEQQHCNETFRLCNISPQVGVGFNRDAWNNLEIHVRKLTNSYTHVYCFTGPLFLPRREDGKMYVKYEVIGNNNVSVPTHFFKIALLERSDGKYDVESYVLPNENIDNNVPLNNFFEEPETIEKAAGFLFFDNVPKAEVIKINGRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0E0</accession>
    <name>T1H0E0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PARP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02377063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0E0"/>
    <dbReference type="STRING" id="36166.T1H0E0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009615-RA">
        <property type="protein sequence ID" value="MESCA009615-PA"/>
        <property type="gene ID" value="MESCA009615"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2549513_0_0_1"/>
    <dbReference type="OMA" id="FPPPHIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PARP-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009615-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9479" checksum="3CD66778AD4529D8" modified="2013-10-16" version="1">MSDSEKPFAIENAKTGRAACKKCKEKCETGQLRIAKLVANYFSDSGMMKHWFHLDCLFKAFPPPHILERKLLMSSFDPCRLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0I7</accession>
    <name>T1H0I7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02118261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02118262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02118263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0I7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009666-RA">
        <property type="protein sequence ID" value="MESCA009666-PA"/>
        <property type="gene ID" value="MESCA009666"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266809_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009666-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11495" checksum="89E5DF80D003A120" modified="2013-10-16" version="1">MFRSVAAEIEVAFVSSSVLNLPFSFPSDLLKGLMSTPDFSSTFFRSCVAPGPYMVLVSQVAAFISLSIRVDCRHEGVSTRAMCNLSDSDRHRWTNYFITYVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0J9</accession>
    <name>T1H0J9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0J9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009679-RA">
        <property type="protein sequence ID" value="MESCA009679-PA"/>
        <property type="gene ID" value="MESCA009679"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078918_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577885" evidence="1">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009679-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13130" checksum="A3807AD66EE3C75C" modified="2013-10-16" version="1">MKYISCCILVIIQVVLVFCSIKDNPPIGGFPASSTAGTFVALAIPLDLPHRNVYVSYNFEANYNIIGSAEDVLPGPLHFFLPTKLKEPIDTTPSPNAYWSSLQWPYGTISRNYHKKEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1M1</accession>
    <name>T1H1M1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA2/NAM7 helicase helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02127610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02127611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1M1"/>
    <dbReference type="STRING" id="36166.T1H1M1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010085-RA">
        <property type="protein sequence ID" value="MESCA010085-PA"/>
        <property type="gene ID" value="MESCA010085"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1557042_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF419">
        <property type="entry name" value="RNA HELICASE MOV10L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="1">
        <location>
            <begin position="80"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010085-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="19601" checksum="99A688627B30A8D5" modified="2013-10-16" version="1">MNNEVLFTVSAGKSEYKIVSGSDFNIRFVPNRYQLLCQYAALNKTKTNLLRYLFPTDNRNFNNILQKGAIKLNNISIEKNPEQLQAILQICNDGWSNKCPYIILGPPGTGKTTTIVECIVQLVSNSENNRILVTAPSNSACDEITMRLRKILYKGYDQKPLLLRIYSQTYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1S9</accession>
    <name>T1H1S9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CYRIA/CYRIB Rac1 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYRI family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1S9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010147-RA">
        <property type="protein sequence ID" value="MESCA010147-PA"/>
        <property type="gene ID" value="MESCA010147"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129362_0_0_1"/>
    <dbReference type="OMA" id="WTNIEDV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039789">
        <property type="entry name" value="CYRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12422">
        <property type="entry name" value="GH09096P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010147-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15464" checksum="634A5ADCD2FD3063" modified="2013-10-16" version="1">MSLFYAHATPMLKVLSEATSKFVHDNSNDVENTTETIGTMAKVCLRMLENPKLLQQIEREETHLLVLRVMVGLVILYDHVHPVGAFVRGAHVDVKGCVRLLQAQPAVKAEPLLNALRYTTKHLNEENTPKNIRNLLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1X0</accession>
    <name>T1H1X0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1X0"/>
    <dbReference type="STRING" id="36166.T1H1X0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010193-RA">
        <property type="protein sequence ID" value="MESCA010193-PA"/>
        <property type="gene ID" value="MESCA010193"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046341_8_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF21">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 11, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010193-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="11648" checksum="7925357B7CC416E1" modified="2013-10-16" version="1">TVPRTEILGQPDRYIKAGSNVVIRCIVRGALEPPNYIMWYHGSKQLPTDTSRVHTQLDKNLPENDGDSLSTIGSLIIEKVKKHDTGNYTCSPSNSPSATVTLNVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2D0</accession>
    <name>T1H2D0_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <taxon>Megaselia</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2D0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010370-RA">
        <property type="protein sequence ID" value="MESCA010370-PA"/>
        <property type="gene ID" value="MESCA010370"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608743_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010370-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8867" checksum="7F50B7593496CF9A" modified="2013-10-16" version="1">MKSLCNGKISSKNYLTAMTVVLPLKEISPRSFKTTKKSLNQQELKWIKLCRFKNNKSADPEVEGPPLVDLGILLQGGYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2U5</accession>
    <name>T1H2U5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379957">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2U5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010550-RA">
        <property type="protein sequence ID" value="MESCA010550-PA"/>
        <property type="gene ID" value="MESCA010550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294832_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11753" checksum="2AA4FBB90050B529" modified="2013-10-16" version="1">MTRLEESLVFNSNEDVPSPTRDKKECKNYRGISLLNIANKILATILCERLNPSCVWNPWVYRFISENTNILTRFKKSLLSQQQGGVEQNKRNSSVPKLLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1H367</accession>
    <name>T1H367_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02180449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H367"/>
    <dbReference type="STRING" id="36166.T1H367"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010684-RA">
        <property type="protein sequence ID" value="MESCA010684-PA"/>
        <property type="gene ID" value="MESCA010684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_6_1_1"/>
    <dbReference type="OMA" id="IRVHIDG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="288"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="311"/>
            <end position="312"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="373" mass="40894" checksum="E79495661446B275" modified="2013-10-16" version="1">MSFIRTHSVVLSLSTKRDLKDIRSYNVDQITCFVGSKLLYCGCQIEKAQKISRKTDKRALEDILIRPSYFQDVSNISMKTSILGQELALPFGIGPVAMQKVVHTDGEVASAKAAGSKGSIFVLSSFSTTSIEEIAEKAPETEKWLQMYIFKNRTLNSELIKRAENGNFSAIVITVDVPSRGKVCLQYPDFNLPEDLELENFPEGTENPSELISSAFSLDDLKWIINSTNLPVFAKGILTKEGARMARDAGCKGVIVSTHGGVDRTPSSIESLPEVVKAVGEDTIVMFDSGIRGGDDIYKAIAFGAKYVFLGRPALFGLAVDGQKGVEGVLDTLKDELENVMALSGCASLSDIKRDKVVHWKYYQNLLRSGDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3M6</accession>
    <name>T1H3M6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the even-skipped homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02070609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3M6"/>
    <dbReference type="STRING" id="36166.T1H3M6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010857-RA">
        <property type="protein sequence ID" value="MESCA010857-PA"/>
        <property type="gene ID" value="MESCA010857"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065032_0_0_1"/>
    <dbReference type="OMA" id="LINQQCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000256">
        <property type="entry name" value="Even-skipped homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052002">
        <property type="entry name" value="Even-skipped_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294:SF4">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="69"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="71"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="237"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010857-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="283" mass="31292" checksum="B38D03F45E4F7D89" modified="2013-10-16" version="1">MQGYRNYTMEAHHLSQARDQKPIVDLINQQCYTKPQTPPPSPNECSSPLSSSDASLSSNSLNNQIPSDPSVRRYRTAFTRDQLARLEKEFYKENYVSRPKRCELAAQLGLPESTIKVWFQNRRMKDKRQRIAVAWPYAAVYSDPAFAASILQAAANSVGMPYGYPASPMMPNPMMPAQIPQCAAPTTVSVPPSYSGYRYNPYQIPQRSAPMPPMPYLDRSLSPIRNPSGGLLMTAIPQPQAQSTPISLSSSASSGSAVSSVRVPTVTEPKPKLFKPYKTESEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H412</accession>
    <name>T1H412_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H412"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011010-RA">
        <property type="protein sequence ID" value="MESCA011010-PA"/>
        <property type="gene ID" value="MESCA011010"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870271_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15579" checksum="76E9BD75D33DD484" modified="2013-10-16" version="1">LAGFLVHRIKRSTQSTISRKQLDFAISLNPHDSVTQATEARMKSTFFLIKQLILCLLQKYIQKHDLNVANINTSNIIYTKYNQMLGYADDLDVIGITTRDVANSFLATIKWTKVLLSSRKEASHRRLGENVTIQGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H499</accession>
    <name>T1H499_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02109691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H499"/>
    <dbReference type="STRING" id="36166.T1H499"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011105-RA">
        <property type="protein sequence ID" value="MESCA011105-PA"/>
        <property type="gene ID" value="MESCA011105"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175015_1_0_1"/>
    <dbReference type="OMA" id="ISLCIRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="3">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011105-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8627" checksum="70E8578A2C47C23C" modified="2013-10-16" version="1">MKVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSILVFYLPSDSGEKISLCIRYYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4R5</accession>
    <name>T1H4R5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4R5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011282-RA">
        <property type="protein sequence ID" value="MESCA011282-PA"/>
        <property type="gene ID" value="MESCA011282"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123898_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011282-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="13025" checksum="06AFCD6F5CEAFF1F" modified="2013-10-16" version="1">MLVQTVFSTNQIGDRRTRRANQRKDDKRNAQNRSIWRIFVNQAEFAVATKVKSDDIDLISRLSGNVADYFLRFEQAEKSVGLQDNGNQTKYLLSTSKQTAIEPVISTCIPITSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H515</accession>
    <name>T1H515_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02156204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H515"/>
    <dbReference type="STRING" id="36166.T1H515"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011388-RA">
        <property type="protein sequence ID" value="MESCA011388-PA"/>
        <property type="gene ID" value="MESCA011388"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1135899_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011388-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="27534" checksum="8DEBF7A33A8019B7" modified="2013-10-16" version="1">MGELRLIPDKFQNKQRKDFNTETTCIELTHCGNFVIIGYSSGDLEKFNIQSGIHRTTYGPPAHTAPVRGVAIDNLNQFVVSGDGDGIVKFWNFKENVAHPIHKIALSSGVSMFRSHRESAMVCIALEDFTISILDCDTKAIIRRFEGHTARINDATFSPDSRWLVTASMDSTIKIWDIPSSYMVDHFKVERPCVSLNMSPTGDFLATSHVNHLGVYLWANKKLFNHISLRSINPYSEAPYIDLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H561</accession>
    <name>T1H561_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H561"/>
    <dbReference type="STRING" id="36166.T1H561"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011436-RA">
        <property type="protein sequence ID" value="MESCA011436-PA"/>
        <property type="gene ID" value="MESCA011436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514290_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF211">
        <property type="entry name" value="LD43457P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="19767" checksum="0A6345F11535A311" modified="2013-10-16" version="1">VGLTESVRGDQKRFELWLQGRQEVYTLQAATIEIKNKWVTEIKRVLFNQLEELKGEKIKQYGQNCRHIKQMSSWDTSNTSISLPQRQNISCDTSSESNRNSNGSGGIDDHGPPLTPGIHSLSSSNNDGSNDACGWSSDYSNSDDELSIEENSTPTLLNILAQWASSTSSNETHGLYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5H1</accession>
    <name>T1H5H1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemocyanin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378750">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5H1"/>
    <dbReference type="STRING" id="36166.T1H5H1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011548-RA">
        <property type="protein sequence ID" value="MESCA011548-PA"/>
        <property type="gene ID" value="MESCA011548"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592481_0_0_1"/>
    <dbReference type="OMA" id="TYENMSM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1520">
        <property type="entry name" value="Hemocyanin, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005203">
        <property type="entry name" value="Hemocyanin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037020">
        <property type="entry name" value="Hemocyanin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF24">
        <property type="entry name" value="GH04080P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03723">
        <property type="entry name" value="Hemocyanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemocyanin C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011548-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9132" checksum="A71EFA8CE27C0F2D" modified="2013-10-16" version="1">MISDYTGDEVLQTNPTDVCADSSSFCGLKDQLYPDKRSMGFPFDRTLNGDDLQTFVETYENMSMSNIVIKFTDTIVDRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5H3</accession>
    <name>T1H5H3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5H3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011550-RA">
        <property type="protein sequence ID" value="MESCA011550-PA"/>
        <property type="gene ID" value="MESCA011550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1212414_0_0_1"/>
    <dbReference type="OMA" id="PRTHSEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF84">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="3"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="229" mass="26808" checksum="1F14F410D3C0BC6B" modified="2013-10-16" version="1">ICVVLAAVLGTIIYRISLVSVIYGGGGFFVKKHAKLFTTVTAAIINLIIYHRLAVYLTNLENPRTHSEYEISYTFKIFIFEFMNFYSSLIYIAFFKGRFFDYPGDDVARKSEFFRLKNDICDPAGCLSELCIQLAIIMVGKQCWNNFMEYLFPKFYNWWRQRKHKAATKDETHMHMAWEQDYHMQDPGRLALFDEYLEMILQYGFVTLFVAAFPLAPLFALLNNIAEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5N2</accession>
    <name>T1H5N2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP binding protein second domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5N2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012015-RA">
        <property type="protein sequence ID" value="MESCA012015-PA"/>
        <property type="gene ID" value="MESCA012015"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412104_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP binding protein second" evidence="1">
        <location>
            <begin position="81"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012015-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="21981" checksum="5DBE95ED0DF740B8" modified="2013-10-16" version="1">IARTQKNKATEYHLGLLKAKLAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYKIFNAEVLFREDCTEDEFIDVISANRVYLPCLYVYNKIDQISIEEVNVSQTGTLCVVIAIHLNLDYLLEVLWEYLQLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6D6</accession>
    <name>T1H6D6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Vesicle protein sorting 29</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the Csubcomplex and the retriever subcomplex. Component of the retriever complex, which is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos. Component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. In the endosomes, retriever complex drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to snx17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. The recruitment of the retriever complex to the endosomal membrane involves Cand WASH complexes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS29 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6D6"/>
    <dbReference type="STRING" id="36166.T1H6D6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012269-RA">
        <property type="protein sequence ID" value="MESCA012269-PA"/>
        <property type="gene ID" value="MESCA012269"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2405695_0_0_1"/>
    <dbReference type="OMA" id="YVSSEHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012269-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10343" checksum="CDC21CC4A7A4784B" modified="2013-10-16" version="1">LANDVHVVRGDFDENFNYPEQKVVTVGSFRIGLCHGHQVIPGDPEALALIQRQLDVDILISGHTHKFEAYEHENKFYINPGSATGAYHALNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6F4</accession>
    <name>T1H6F4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6F4"/>
    <dbReference type="STRING" id="36166.T1H6F4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012287-RA">
        <property type="protein sequence ID" value="MESCA012287-PA"/>
        <property type="gene ID" value="MESCA012287"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122205_0_0_1"/>
    <dbReference type="OMA" id="WHEKGAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF15">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012287-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14306" checksum="757BFBBC119AEFED" modified="2013-10-16" version="1">SIQKALNGLEVPLKVKHARAIIISTFRTAMFLWEMLKTQPLMEQRFTAWKFCHLLHKVLREGHHSIISAQSKIESILQIFDVGASEDGVGICIDLYSKLLVMKIDFHRKNPAIPGSMLFEFNDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H755</accession>
    <name>T1H755_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum-Golgi intermediate compartment protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ERGIC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H755"/>
    <dbReference type="STRING" id="36166.T1H755"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012537-RA">
        <property type="protein sequence ID" value="MESCA012537-PA"/>
        <property type="gene ID" value="MESCA012537"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034705_1_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="8">
        <location>
            <begin position="129"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012537-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="28195" checksum="2A62AB0BAAC480E8" modified="2013-10-16" version="1">KISNILRGFDAYPKTLEDFSIRTLAGALIISIRITLEFLAFLRPDLQEKLFEDTSRNNLLRINIDFYVKNLSCSDAVDSSGDQHIQIQHNIFKHRLVLEGNPIKEDQHPIKEIVMATSLSQKYNCGSSYKAQQNSSHCCNTCESVLEVYRIKKWDLDIKKIEQCKGQEDKFKRHDKYAFKEGRIKGQLEVNRMPGSFHVPGASFLISQFHIHNFQVSDFKLMHRINHLFFGEKIDFAKTNPMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7M1</accession>
    <name>T1H7M1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H7M1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012758-RA">
        <property type="protein sequence ID" value="MESCA012758-PA"/>
        <property type="gene ID" value="MESCA012758"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217367_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="3">
        <location>
            <begin position="47"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="49"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012758-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="25414" checksum="B8563B5F678218D1" modified="2013-10-16" version="1">MGISEEVKLDEIPQEAQDSLPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVWFQNRRAKWRKREKFSPHHMISPSAMAGMYSPSTSFQSLLANISAVPRPPPQVQSPKQLVVPDNVISSPPTSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Z4</accession>
    <name>T1G9Z4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YqaJ viral recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Z4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000036-RA">
        <property type="protein sequence ID" value="MESCA000036-PA"/>
        <property type="gene ID" value="MESCA000036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1367610_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019080">
        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39953">
        <property type="entry name" value="RE54151P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39953:SF1">
        <property type="entry name" value="RE54151P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09588">
        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YqaJ viral recombinase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="22861" checksum="5F72CC78E24B0DAA" modified="2013-10-16" version="1">MFCRSECGILLNENYPYLCASPDGITDDHIVEIKAPRNDDEFEKYLEGKETISAKYMAQIQIQMYMANVTKALYCVLSPTFDTNGALHYVWVQADMDFVESLLSTAEDYWRDIVFPKICSIYIHISMDISFIHAHVPIEGSDGTENDIFYDQLERILDTISAYTLKIVLSNLNAQVGKEQHFPDTISTKAIARDTMQQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA32</accession>
    <name>T1GA32_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000077-RA">
        <property type="protein sequence ID" value="MESCA000077-PA"/>
        <property type="gene ID" value="MESCA000077"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029357_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000077-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13749" checksum="511B142B1EB83A8C" modified="2013-10-16" version="1">MEEEDVEIMETNEGSRTASKEETPWMNESEIHHEEIVYADDIPTSEEVVVNEAEDQLLETACFETIPSGGNLKYIGPRSARSLLKVQFAPISKPPPSKFKSTLSVHPETLKGKFPELFDQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAA2</accession>
    <name>T1GAA2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02031884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAA2"/>
    <dbReference type="STRING" id="36166.T1GAA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000152-RA">
        <property type="protein sequence ID" value="MESCA000152-PA"/>
        <property type="gene ID" value="MESCA000152"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827138_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="99"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000152-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="336" mass="38282" checksum="83C748D11B59D1FC" modified="2013-10-16" version="1">MNRITPYCRTTCGWNCQLPGHRVSNCPEIKKIFCSFCLRKGTTTLACKCRQPGPSNAKPPTYHRINPPIRYNDIKKYPVCPMGNNVRIYPIGVFIGDEELLKLFLEQFGIKSCILNSELPTSIRCHVISQFNQGKYDIIIASDEKMLDDAAGGSSKKDKESGVSRGIDFQSGRTARGTNKGSVLSFVSIKEQPMNAKVEERLKAGYSSEETIIRNYQFKMEEVEAFRYRAHDAWRAITKVAVHEARLKEIKQEIFNSEKLKGFFNDNSRELEVLRHDKPLKTVKVQQHLSTVPDYIVPNSLKRIANSTNKRTRSTQFKKYSLAKAAFESQMKDPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAF9</accession>
    <name>T1GAF9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAF9"/>
    <dbReference type="STRING" id="36166.T1GAF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000215-RA">
        <property type="protein sequence ID" value="MESCA000215-PA"/>
        <property type="gene ID" value="MESCA000215"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_2_4_1"/>
    <dbReference type="OMA" id="SHKILCQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000215-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="10931" checksum="A8B3607D09EC8553" modified="2013-10-16" version="1">MEYRSFGKTDMLVSKLAIGGATLSSLFGQLLKDPNVLFAGYKIPHPLEHKFVIRIQTTSDYSPHDAFMHAITDLIAELSLFEERFKEAIKEKKEGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB66</accession>
    <name>T1GB66_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000488-RA">
        <property type="protein sequence ID" value="MESCA000488-PA"/>
        <property type="gene ID" value="MESCA000488"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375157_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000488-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10812" checksum="6D28F29F6F980EC3" modified="2013-10-16" version="1">MGCTCAEDGRKCLSTNPNGYRKKGRPQTRWSDIISHDDRAIGVPIWRTSAKSMIKRNLNEGSSSQKIYVELAEEVVRKFSATPGKREFEYGRKEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBJ8</accession>
    <name>T1GBJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02192470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GBJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000632-RA">
        <property type="protein sequence ID" value="MESCA000632-PA"/>
        <property type="gene ID" value="MESCA000632"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460497_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="252"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000632-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="593" mass="64463" checksum="7CB39351451DF4E5" modified="2013-10-16" version="1">SAPLPLGHPSLVGTGPINIDQTHYEETEAERQAYDQQKHLALTKKIRETVLIRSGQISSRDTSLKEEAESSEVMDLTNKNQPPKSISMQTTTSHSLSDIMNNHGNHSTEKLRDQDYLQKQREILFTQRAAAGLDPRNLSRPLSRTLSSPLVPQHQILSDTGQAMSIHASPIMTSSSADNIPPINLSLSHQRKFIGLDNFRSQVLAASSLSSATSSSSTIASAVSPPLHLTTGLAYDNTMLKHACTCGNNSVHPEHSGRLQSVWARLNETDLASRCHRLRSRKATQEELQFRTNGCGGXYGSNPISRQKLDMSSMPSFVQMACGGVGVDLDTTWNDVHTAAAARMAAGCVIDLAFKCAKGEVRNGFAVVRPPGHHAEPNSPMGFCFFNSIAIAAKLLRQKMGNEIQRILIVDWDVHHGNGTQQVFYDCDDVLYLSIHRHDDGNFFPGTGGPTECGADRGLGFNVNISWSGGLEPPLGDAEYLAAFRTIVMPIAREYNPDIILVSAGFDAAAGHAAPLGGYMVSPACFGWMTKELMQLADGKVVLALEGGYDLPAICDSAQECVKALLGDEISPLNEIELNRRPCQNAKILYRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBZ4</accession>
    <name>T1GBZ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase-like protein 22</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02076762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBZ4"/>
    <dbReference type="STRING" id="36166.T1GBZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000787-RA">
        <property type="protein sequence ID" value="MESCA000787-PA"/>
        <property type="gene ID" value="MESCA000787"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048361_1_0_1"/>
    <dbReference type="OMA" id="EYERCPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038899">
        <property type="entry name" value="METTL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108:SF0">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000787-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="31016" checksum="45602EB28BDB984F" modified="2013-10-16" version="1">MISHILYSVDNLFPSRKHIMYEVTSEIYSETDFQTTNSSDGKIVSTFKFVYPTEVRSATKDKDGDFEVELLKLVGLQVWRGALLLADFIFTNRKEFQNYEILELGAGVGLTSIAAAIYSKQVFCTDINLGGILETISSNVKRNKSIIKNPMSVLEFDFKAETYAEELKEAINKSDIVIAADVIYDDDLTDCFIRVTDILLQNPKKKTVYIALEKRYVFTLSDLETVAPCFEYFLTKISKKPWKVNYIPIDFPQYFTYKRCKDLVLLKIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD68</accession>
    <name>T1GD68_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GD68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001249-RA">
        <property type="protein sequence ID" value="MESCA001249-PA"/>
        <property type="gene ID" value="MESCA001249"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1191062_0_0_1"/>
    <dbReference type="OMA" id="RQISHIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF150">
        <property type="entry name" value="FI01825P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="10"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001249-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="233" mass="25571" checksum="4C235EB37F3A2602" modified="2013-10-16" version="1">MGINYEIRSXSLGAHVVGFAGKNVKKSGNIIREIVGLDPAMPLFNYDKPDKRLNSEDGYYVETIQTNGGELGFYQPIGKAAFYPNGGKTQPGCGIDLSGGCAHERAVDYYAEALRTGNKNKFDVAKCPDFSDLKSVNCHIRATDVKMGDAENVGKAEGVYYLKTNKDYPYAMGNSLAVKDMTWVPKETQLLLLLLLNLLVYLGFEFVAPKIVEIFQEGFQFQTGVSNLICVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDA4</accession>
    <name>T1GDA4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDA4"/>
    <dbReference type="STRING" id="36166.T1GDA4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001287-RA">
        <property type="protein sequence ID" value="MESCA001287-PA"/>
        <property type="gene ID" value="MESCA001287"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925231_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001287-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6671" checksum="2C383AABA7FFC116" modified="2013-10-16" version="1">MFNFLEIIAITLFAGFIGLVCAVRFYVKLTMGRCTNQNKMEGKTVLITGANSGIGKETPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDM1</accession>
    <name>T1GDM1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093592">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001410-RA">
        <property type="protein sequence ID" value="MESCA001410-PA"/>
        <property type="gene ID" value="MESCA001410"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443360_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001410-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10240" checksum="CAE6B817CBEC936F" modified="2013-10-16" version="1">MVREKKWVLIKPSLKYWGLIEIEIRQETTNLQVDSACSFPIGMSEPYLYDLEAHSELDIVLRTTARIWAGYSFFSVLPHPTTVAVDPAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDU0</accession>
    <name>T1GDU0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60S acidic ribosomal protein P1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02029573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDU0"/>
    <dbReference type="STRING" id="36166.T1GDU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001490-RA">
        <property type="protein sequence ID" value="MESCA001490-PA"/>
        <property type="gene ID" value="MESCA001490"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_1_2_1"/>
    <dbReference type="OMA" id="EYIYAAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001490-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="11412" checksum="583CE5B9258A3A72" modified="2013-10-16" version="1">MTTKAELACVYSALILTDDDVAVTGEKISTILKAANVDVEPYWPGLFAKALEGVNVKDLITNIGSGVGAAPAAAAGAPAAGAAPAEAKKEEKKKEEEPEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE65</accession>
    <name>T1GE65_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02106941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE65"/>
    <dbReference type="STRING" id="36166.T1GE65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001623-RA">
        <property type="protein sequence ID" value="MESCA001623-PA"/>
        <property type="gene ID" value="MESCA001623"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745148_0_0_1"/>
    <dbReference type="OMA" id="EYKFVEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537:SF2">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="95"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001623-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="17020" checksum="EF41082E2BF307D5" modified="2013-10-16" version="1">LKFSEFSIPSDSTGPQNCEEGDSLQILVPIRGKYEIVETLCGDTIPKPVMSNGPKMLVEFRGRISGKGNRGFKAEYKFVENFGIITGQQLSNQDCGFVFNSTKEKTGWFHSPNFPGIYPQNVECNYYFHGRQNERVTIKFSYFDVEGIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEA9</accession>
    <name>T1GEA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001668-RA">
        <property type="protein sequence ID" value="MESCA001668-PA"/>
        <property type="gene ID" value="MESCA001668"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1715344_0_0_1"/>
    <dbReference type="OMA" id="FARNITI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001668-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="18328" checksum="F80EC712AB0905BA" modified="2013-10-16" version="1">MVCDFNENYAENGTCELKGLGRQFIVANLEADVKKPFKNISIHLKMFKFYNQFRPFLVDVEFNVCDAFNKKSALNFYGNTLMRLMMKYTNIRKCPISGHVFIKNFELKVDYFKNIMEDGKYRFNFMFFETAEWLANFTFYGEILTNYRRPKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF06</accession>
    <name>T1GF06_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tubulin--tyrosine ligase-like protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GF06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001934-RA">
        <property type="protein sequence ID" value="MESCA001934-PA"/>
        <property type="gene ID" value="MESCA001934"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010131_7_3_1"/>
    <dbReference type="OMA" id="GNFRRIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF161">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001934-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="76384" checksum="60E9BC329017E16F" modified="2013-10-16" version="1">MTVDGPMNRKFTKQIENLKLLTKRRNEQKKSSDSSSNTSSRPIGDTGVNGTLCRISEVNVQDFNEETGGGDNECSTGLLKINNSYFKIDVLENPELSKEEESNQLEEYFKMPGYKKGKLSICLTNSRYSVLTRVARQLGYRIVKETHLWNILWADSVPGVEVYKDMKRFQKINHFPGMQEICRKDLLARNLNRMNKLFEEDYDIFPKTFVFPAESKTFILKPDQGAQGRGIWLTKNLKVICPTDRMICQVYINKPLLLNGYKFDLRVYTLITSVDPLRIFVYNEGLARFATSKYKEPTLHNTSNVYMHLTNYAVNKHSHTFCKDEEDGSKRKLSTINRILEQEGYDVFNLWANIDDVIVKTILSSLPVXNRILEQEGYDVFNLWANIDDVIVKTILSSLPVLKHNYHACFPHHDLIQACFEILGFDILIDRNLKPYVLEVNHSPSFHCDEAIDKEVKEALVRDTLNLLNVALIDKRKVINEDRKRVKCRLLKKKNEAFSEIANSKNDEKPSQSLLGQQIAWEETHSGGFRRVMPPSNNINKYCQYFVVKNQASIFSETANSKRREEAAKLIRNSIIEKQKQQQIILKTNTSKDIDVKQKNFKISAKDRKSMEKRRLESLHFQEKWLPNFISENEERLRLDELEKRDKVIREEGLFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFG2</accession>
    <name>T1GFG2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02091797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002103-RA">
        <property type="protein sequence ID" value="MESCA002103-PA"/>
        <property type="gene ID" value="MESCA002103"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006521_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14245" checksum="3ED9901A74D31849" modified="2013-10-16" version="1">MYLGLIRGGLSEMAFSLKCQTSKSVPVIISSFEQEGDELDTGNTEQSYQSYTVEEVKTLIKLMKVTFPKKRIPIRRKKSLDEVNVPGHENMLKNDDLSCLLAKRRSSVYIFHKLINKSHMFDFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFG4</accession>
    <name>T1GFG4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFG4"/>
    <dbReference type="STRING" id="36166.T1GFG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002105-RA">
        <property type="protein sequence ID" value="MESCA002105-PA"/>
        <property type="gene ID" value="MESCA002105"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691152_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019321">
        <property type="entry name" value="Nucleoporin_Nup88"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10168">
        <property type="entry name" value="Nup88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002105-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="17497" checksum="C7EB4E113DFF2825" modified="2013-10-16" version="1">ILTFLIFRVYKDTELKHIWRVGPVLNNCSATNKLNTSLPTLSNLGECVIDFDIFPMQISLPKSKQNPNIRYTWPIALLQENGNIFISNASLETLKPKIGQAILIKPKDRRNYGLDSCSIMFMLSSAPTLVIAETSGKIHHALLLQKQDSEDVSIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFR6</accession>
    <name>T1GFR6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFR6"/>
    <dbReference type="STRING" id="36166.T1GFR6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002213-RA">
        <property type="protein sequence ID" value="MESCA002213-PA"/>
        <property type="gene ID" value="MESCA002213"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1490657_0_0_1"/>
    <dbReference type="OMA" id="CFGESYT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000043">
        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002213-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20611" checksum="EA52AE67E664843C" modified="2013-10-16" version="1">MGGGGGRNDDFFIGRRLQEMQGPTFELPELKNDEIKFSGRNRLYIGNLTNDVNESELIELFKPYGEISETFLNNEKNFAFLKLDFHANAEKAKRELDGTQRKGRSLRNLTPFVSNELLYRAFEVFGPLEKATSIADDRGKHTGEGIIEFVKKSSAMSCLRLCSEKCYFLTSALRPKKRLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFU3</accession>
    <name>T1GFU3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094965">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFU3"/>
    <dbReference type="STRING" id="36166.T1GFU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002243-RA">
        <property type="protein sequence ID" value="MESCA002243-PA"/>
        <property type="gene ID" value="MESCA002243"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045884_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070976">
        <property type="term" value="F:TIR domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035325">
        <property type="term" value="F:Toll-like receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002755">
        <property type="term" value="P:MyD88-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008063">
        <property type="term" value="P:Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017281">
        <property type="entry name" value="Myelin_different_resp_MyD88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15079">
        <property type="entry name" value="MYD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15079:SF3">
        <property type="entry name" value="MYELOID DIFFERENTIATION PRIMARY RESPONSE PROTEIN MYD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="123"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002243-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="32468" checksum="661DC5E5BC820356" modified="2013-10-16" version="1">MMFNTSQPPASTRYLRIPLKHLTDKTRSVLSVSLNNKKHIRAENGFCRDWRGIIELCGLRKGYYDIVDNSSDHMEKLLELWCLKEGDLCPKANKPITSPLPVERNEVLTLQDLKRIEEGKPLRNYDAFLLFEDEDAEFVQTLLAKLSNYHICMKSDLVRQTFEHTAVVELIEKRCRRVIAVISEHFLYSPSNANRFLVDFAQASQVESGDLRIIPVVTEREVEKYIPSNLKYLHKVQYYKNSPLYDFWRILESTLMDKSALYNKMQQITMAESVRKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG30</accession>
    <name>T1GG30_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002336-RA">
        <property type="protein sequence ID" value="MESCA002336-PA"/>
        <property type="gene ID" value="MESCA002336"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870166_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002336-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="8098" checksum="844AFFED41A7ECFF" modified="2013-10-16" version="1">MAKLKKRRFEKQQFEEHEFMRDRHQAMKFYEHVNKQWKKITPQQFPPLSERLEPHVIRIFGPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG53</accession>
    <name>T1GG53_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG53"/>
    <dbReference type="STRING" id="36166.T1GG53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002362-RA">
        <property type="protein sequence ID" value="MESCA002362-PA"/>
        <property type="gene ID" value="MESCA002362"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_824621_0_0_1"/>
    <dbReference type="OMA" id="LYSAYNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038602">
        <property type="entry name" value="Mite_allergen_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020234">
        <property type="entry name" value="Mite_allergen_group-7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF29">
        <property type="entry name" value="IP17226P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16984">
        <property type="entry name" value="Grp7_allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002362-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="337" mass="39070" checksum="2DCED2C1A0AB752D" modified="2013-10-16" version="1">MYELKNSSQLKILPVRSSGNIKLRIENISAKGLVAFKIYDDALKTTNFNLDFDSQDVHIEIDYYDQESKEYAKSVQTKKNIDCSIATHIKRDLIWKLQEDLRHRLDAVLVDFSLTEIFNENLEISEKYRNRNLFIDKFINEFVDQFLEEIKKVEMAKNLTKVKTPDFETDFSHKFGVITFWGNFQAKNGTLEDLSSVFRTGEFSLATYPNSNKVLVYGNLGFENLLLQFDYYTAKIWNIGPWGELKATIGNNSIKFKLVAEVQPSDDTHLGLNMKFSDFDLKIEQMDDIKVECTGMSVILNWLLSYIVTWVTGYLKQDIIEIVEDKLKDLLESCCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG65</accession>
    <name>T1GG65_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02031787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002375-RA">
        <property type="protein sequence ID" value="MESCA002375-PA"/>
        <property type="gene ID" value="MESCA002375"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690669_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002375-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8737" checksum="AC38BB5CF002AE4F" modified="2013-10-16" version="1">MIIEFEPKLTDFKDRLMETKSSQSRVYLVYARTVCYLRNRAIWGASTLHMIELLPFFPHDLLMLDISLIFLPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGD3</accession>
    <name>T1GGD3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="Alpha-tectorin"/>
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        <property type="entry name" value="PROTEIN PRY2-RELATED"/>
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        <property type="entry name" value="VWD"/>
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    </dbReference>
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        <property type="entry name" value="Serine protease inhibitors"/>
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        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
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    <feature type="domain" description="VWFD" evidence="2">
        <location>
            <begin position="304"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002449-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="365" mass="41196" checksum="40D1605BAD2549B2" modified="2013-10-16" version="1">MKSIFYFKIHPVQQNCTKESKVCKKELTIRDKDADAVLTILPTMKLRFNGFIYTVRQLQKAPLAKESFIISQPGSTILIVAKNHGFWVSYDENNSEIKVGVSARFKKTVDGLCGYYNGYNKDDKRMPDGNLTDNTRIFGDSWYDKSVPKEECYQQVCPRSVQAKALKLCNAIKHPNFLACDKAVNYKQFVSKCIESTCECLQQNGGNEKSCKCNILQGFVKQCLAVKPNVKLDTWRAVHNCEISCPAPLVHSDCFKRRCEPSCENINGDGCPVLPDACFTGCYCPEGTVRKGDDCVPVTECKDCVCDSYGKSKYFTYDRQNFTFNGNCTYLLSRDIILPGVHTFQTHGLSCQDSWLEVGSFRLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGJ4</accession>
    <name>T1GGJ4_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Megaselia scalaris</name>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA002515"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002515-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="6730" checksum="C6B5FB1A36488EED" modified="2013-10-16" version="1">MGTGIGMGIGIGTGNGVAYMCIVSLVAAAAAAEAAVEAAVLFLLVGGGKLSALRFTDISVFPRFGGLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS5</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <lineage>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <property type="gene ID" value="MESCA002604"/>
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            <dbReference type="EnsemblMetazoa" id="MESCA002604-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="54" mass="6301" checksum="1A14814C0D3272E5" modified="2013-10-16" version="1">MMKLTFQPIQLNVIKIWKRNYIKAKGFTNVPVYYNGGSYDCVFIVIDEDCVPLL</sequence>
</entry>
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    <accession>T1GH66</accession>
    <name>T1GH66_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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            <taxon>Megaselia</taxon>
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    </organism>
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            <authorList>
                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH66"/>
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        <property type="protein sequence ID" value="MESCA002752-PA"/>
        <property type="gene ID" value="MESCA002752"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002752-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14345" checksum="D653F4A7B7C5900A" modified="2013-10-16" version="1">MSIITSRAAMGVDLMHLRVPNRANSWTPDIIALADAILVIFLLLAIHEIIENIQNFGASHLRTNSGTKFKENEKKLQSDKFVFTTPPKVAPLTALLTHFLQKTPQVKVNLENRVNELTIESKIMRKQ</sequence>
</entry>
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    <accession>T1GHE5</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="MESCA002841"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBP/GOBP"/>
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    </dbReference>
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        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17296" checksum="A6C135EF8BBA29D3" modified="2013-10-16" version="1">MFDYLDDIGTHGDDWFYYGVLQVKVVRGPSFSDCCDDRIYTEEAHSAVHGLYVKCKEENGDEASSCIHTCVFRNLGFYSDNGIDYTAMKQMLGPSSMIGEPNDWKKTGIEKWIDECSRAAQGGQECSQDVKDLTQCFWMKVFTNCPSYNAANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHU3</accession>
    <name>T1GHU3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHU3"/>
    <dbReference type="STRING" id="36166.T1GHU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003004-RA">
        <property type="protein sequence ID" value="MESCA003004-PA"/>
        <property type="gene ID" value="MESCA003004"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_9_3_1"/>
    <dbReference type="OMA" id="GVNFTMF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000010">
        <property type="entry name" value="Myosin regulatory light chain 2, atrial isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000007">
        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003004-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="20022" checksum="7744BF4C5DF61175" modified="2013-10-16" version="1">MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPDDEYLENMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEDNNGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYIEFTRILKHGAKDKDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHX4</accession>
    <name>T1GHX4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003037-RA">
        <property type="protein sequence ID" value="MESCA003037-PA"/>
        <property type="gene ID" value="MESCA003037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1379555_0_0_1"/>
    <dbReference type="OMA" id="HKVVYDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF1">
        <property type="entry name" value="MD-2-RELATED LIPID-RECOGNITION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577379" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22667" checksum="16C45D8B7CDA72A0" modified="2013-10-16" version="1">MESKASIVLQVLFVLLYVHLSSGVNVIRPKFESIDLWSDNEYLNYTFAMDGETPYFNFTFNVLKNLPDIEIHVEGTLQNKDDMNHYFSLNTTYNLCKVFEFKDKSPIGVLVWSFLNDHGSLPARCPIPAKTYSIHKIWIPEEEATGVINEILFKLNVQATVKLPTPKLILNDTYKGVTYIKSVSSIQQGILSMLKLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI24</accession>
    <name>T1GI24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI24"/>
    <dbReference type="STRING" id="36166.T1GI24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003091-RA">
        <property type="protein sequence ID" value="MESCA003091-PA"/>
        <property type="gene ID" value="MESCA003091"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033021_0_0_1"/>
    <dbReference type="OMA" id="CHELGSC"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="2">
        <location>
            <begin position="6"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="1">
        <location>
            <begin position="317"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="474" mass="53282" checksum="F2E7C2ABA0D4F94A" modified="2013-10-16" version="1">MAHTKLDFEVACLDLSSVENENFVSVGLWTDISIIVLSVPDLKILHREKLGGEIIPRSILLTCFEGIYYLLCALGDGSMFYFLFNMENGSLSEKKKVTLGTQPTILKTFKTLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVTHMCSLNAEAYPDSLALATKNSVILGTIDEIQKLHIRTVHLGEGPRRIAYQESSQTFGVITIRHDIQESTGLVPSRMSASTHAQNITYSTNVISKPGNSNEIGQEVDINNLLIIDQNTFEVLHAHQFMPSEYIISLMSANLGDDGKSYYVVATALVYHEESEPKVGRIIVFHFVRLYEFTNEKELRMECNIQNNISVLYLKSKGDFILVGDLMRSITLLQHKQMEGIFVEIARDCDPKWMRAIEILDDDTFLGSESDGNLFICQKDSFYKSQKSDADGFIDGDLIESFLDLNREKMAETVNGLEKVTAGKKEEVSIDDVIKIVEDLTRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIU5</accession>
    <name>T1GIU5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003380-RA">
        <property type="protein sequence ID" value="MESCA003380-PA"/>
        <property type="gene ID" value="MESCA003380"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906677_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003380-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6960" checksum="C56A6378A739DCB0" modified="2013-10-16" version="1">MPRLCRLNNVGVLVEVSVCTSERAYIECTHQWFLGCEQNNAVDGVEVALLAAMLRFYQVSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIV2</accession>
    <name>T1GIV2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003387-RA">
        <property type="protein sequence ID" value="MESCA003387-PA"/>
        <property type="gene ID" value="MESCA003387"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514286_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576967" evidence="2">
        <location>
            <begin position="19"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003387-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="19619" checksum="058C4E67C95BA15E" modified="2013-10-16" version="1">MKLTLFLLGLAALACVSAYEDNEEYDVLPFFPITEEEYAALRHLGRGSNVISERKLARSGFFGIQGFFDSIKNKNKPAKWGNNGDGNTNNGNNNNNNNNGGNKWGNKWGNKQGNNNGGNTSNKNGGNKGNKRRNKQPRNEIQLIRVPRQATNPNTPDFTNVKGISCIVIVNPKPTKMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIW3</accession>
    <name>T1GIW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02114466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02114467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIW3"/>
    <dbReference type="STRING" id="36166.T1GIW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003398-RA">
        <property type="protein sequence ID" value="MESCA003398-PA"/>
        <property type="gene ID" value="MESCA003398"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590540_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.10">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000896">
        <property type="entry name" value="Hemocyanin/hexamerin_mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005204">
        <property type="entry name" value="Hemocyanin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036697">
        <property type="entry name" value="Hemocyanin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF24">
        <property type="entry name" value="GH04080P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00372">
        <property type="entry name" value="Hemocyanin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03722">
        <property type="entry name" value="Hemocyanin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48050">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemocyanin N-terminal" evidence="2">
        <location>
            <begin position="39"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin middle" evidence="1">
        <location>
            <begin position="111"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003398-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19151" checksum="CE46E8CAEF3AA6FC" modified="2013-10-16" version="1">MFYPSLPYCLGLNKLGNSRMGLNGLGDLKISKPDLSFITLSKKQDFSIFNCNHKQQAARLIDLFMDAPDRYILASLAAYCKDRINPMLFIYCYTVAIEHRPDTKDIYIPSIVETFPLSFIEPSIFNDAREEAAVINPGNRVIKDIPKNFTSTSLEPEQRLAYFREDIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJL7</accession>
    <name>T1GJL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJL7"/>
    <dbReference type="STRING" id="36166.T1GJL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003667-RA">
        <property type="protein sequence ID" value="MESCA003667-PA"/>
        <property type="gene ID" value="MESCA003667"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901476_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040316">
        <property type="entry name" value="INTS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029444">
        <property type="entry name" value="INTS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029445">
        <property type="entry name" value="INTS5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31697">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31697:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14838">
        <property type="entry name" value="INTS5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14837">
        <property type="entry name" value="INTS5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 5 N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 5 C-terminal" evidence="2">
        <location>
            <begin position="74"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003667-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15484" checksum="A37EE424D5708565" modified="2013-10-16" version="1">MKILLGITATCFRKSRNDCDTDNCIQILLSTFRKYSPAFDWVVSRLASCFPLQIITQILRCGLRKFCEDSNSRFHSEVGILEYLSFAHENELKKAIFSMMEEGFLPNNSNQLDIIPFLLEVCNYSEIILHYISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJY7</accession>
    <name>T1GJY7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJY7"/>
    <dbReference type="STRING" id="36166.T1GJY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003797-RA">
        <property type="protein sequence ID" value="MESCA003797-PA"/>
        <property type="gene ID" value="MESCA003797"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="OMA" id="FIFNEWV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="11"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003797-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="259" mass="28077" checksum="56D5B90C3598DDF8" modified="2013-10-16" version="1">FKPSFPIESRVVNGENAAPGQAPYIVSISSPWSTNYSHYCGGSIISKQWILTAAHCLTGSGPVRRAVFAGITNVADRAKGQEVIANYAYAHEKYSGGVGPYDIGLLHLETPLVFNDRVKPIALPYESEIVEGEASLYGWGQTNSSLPSPDPLQRVDTKVVEFKECKAALPSGANIHESNVCSSSLNSKVSACNGDSGGPFVKEDKNGVIQQIGIVSWGYVPCGEANLPSVYTRVSSYTEWIVQVQADYFQNRNFCKQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK52</accession>
    <name>T1GK52_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK52"/>
    <dbReference type="STRING" id="36166.T1GK52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003867-RA">
        <property type="protein sequence ID" value="MESCA003867-PA"/>
        <property type="gene ID" value="MESCA003867"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1499976_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005918">
        <property type="term" value="C:septate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035151">
        <property type="term" value="P:regulation of tube size, open tracheal system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21284">
        <property type="entry name" value="EG:80H7.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21284:SF12">
        <property type="entry name" value="EG:80H7.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003867-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20489" checksum="E6B5D69B8BE5F324" modified="2013-10-16" version="1">EYYVIREYLVPGWLISVQFFVTIAFVLGIIALGILSLVIIRLPLKAVLQYEWIFIKSAFICIAISSVFMFLGVVIFGSCAYRRDWLMYPKFNVLSWSYALAVVSFMVLGLAALVLHRESKNAYEIRGEAKNLVMQMEMQEPGFQHSRHHHKMIMESCCSTGFGAIDGLKIHISKNQGELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKC1</accession>
    <name>T1GKC1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKC1"/>
    <dbReference type="STRING" id="36166.T1GKC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003942-RA">
        <property type="protein sequence ID" value="MESCA003942-PA"/>
        <property type="gene ID" value="MESCA003942"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019375_6_0_1"/>
    <dbReference type="OMA" id="INWILTI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF63">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0769">Symport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003942-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="23474" checksum="AE330BDF3989EE1F" modified="2013-10-16" version="1">MHSLLSTVQQLGWYFLTVMLGLFLHGFGTIAILFFLGTRRLPYKFVAKLSQVLATAFGTGSSSATMPLTIKCLDGMGIDPRVTRFVIPVGATINMDGTALYEAVAALFIAQYRKVSYSFGTVIAVSHSSIYGAAGIPQAGLVTMVMVLDTVGLPAKDVSLIIAVDWLLDRFRTTINVMCDALGTILVNHLSKNDLKDHPVNNAEPHEMVEFCVEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKD0</accession>
    <name>T1GKD0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKD0"/>
    <dbReference type="STRING" id="36166.T1GKD0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003952-RA">
        <property type="protein sequence ID" value="MESCA003952-PA"/>
        <property type="gene ID" value="MESCA003952"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419381_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="48"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="275"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="583"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003952-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="654" mass="73478" checksum="6B0BFEE7C0DC21D8" modified="2013-10-16" version="1">MVTLKDDANVEQVIENINNTPFGNGFLSAKLKPMQEDTNVKPECIEPRTLYIGNLSPTINVQTLKEKFNKSIRMDIGYAQKMKFTRWAFVKFENMEDAIEAYKSTVNTMMDNRSLIVRFRRLKNSVNGKTMPKTDEDPKDNKKDENDDDDKKKKEGTIKTTTSLVEEDTNTKNDNEVKNDRNDERCYLEQNKSNSNDSNKSSCNSNNSSYIEEPKQCNTPLRDEVEDESILDEQSIDGYDEDDDDVLPSNLLTALPSSVVLVEPIFEPPMRAEIIMDTPSPSLPSNTPTQDEGTPSHLTEQLLLPSPLLQNVNEDDDEEEEVQQIKKDVANNMPLLDTPQSSLSIQEESLSFDTTPLISGPNGVSDDDEDDDPPKSEVKPFKDISSSSSSSNTNKKFKNSSKKTDHHEMKQSTMQQDTCDITCFFNLDPTRVPALLEAETSDEVMARLSNEATPLPSPLFHDDGDSDYQDYGRTPPKSTTTMNISCSLGHQLQSSKDIAATVSTINSNRKSNSVEKCNNCEQQITPSKEKSPAINKGSARKNFSLDLNGFNCYDWSFSEDDDKEEDLSNCGYIDLDEDICTLSDDETPLPAPKRRKKEKTEESNIECPRVLSELEIKTEKIQANDQEEDITASANEIPQENTNCEFGAFAEEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKJ8</accession>
    <name>T1GKJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02116419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKJ8"/>
    <dbReference type="STRING" id="36166.T1GKJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004024-RA">
        <property type="protein sequence ID" value="MESCA004024-PA"/>
        <property type="gene ID" value="MESCA004024"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_2_4_1"/>
    <dbReference type="OMA" id="ENGSHDW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000012">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF7">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="8">
        <location>
            <begin position="290"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004024-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="44494" checksum="6A70C2C2BA76957D" modified="2013-10-16" version="1">MAPVQHWNKLVESVQNHIKSVNWVTRVDLRDKKVEYINGLGSFKDAHTNKTEKVITADKIVIAVGGRPNYPDIPGAVEHGITSDDIFSLDRAPGKTLVVGAGYIGLECAGFLKGLGYDATVMVRSIVLRGFDQQMANIVADAMVERGINFLHKCIPKSVEKQADGKLLVKWVNTEANTEHSDKQADGKLLVKWVNTEANTEHSDVFDTVLWAIGRKGLVADLKLDNAGVVTKGDKIEVSPAEETNVSNIFAVGDVIYGKPELTPVAIHAGRLLARRLFGGSTQIMDYADVATTVFSPLEYGCVGLSEEDAIKKMGGEDNIEVYHGFYKPTEFFIPQKSVRYCYLKAVAERSGDQKVLGLHYSERSNFWPKKRSERKNYRASEKQKLVAISKFSKGVYVSKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLJ1</accession>
    <name>T1GLJ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLJ1"/>
    <dbReference type="STRING" id="36166.T1GLJ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004395-RA">
        <property type="protein sequence ID" value="MESCA004395-PA"/>
        <property type="gene ID" value="MESCA004395"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661607_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004395-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8536" checksum="39B01149FFADFF3F" modified="2013-10-16" version="1">MESPNPETKPEQKKEGFENVKRMFQTDEFGTISAELNSIYQAGLFGAFVGAVYGGILYSREGYIDFLEKNQATAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLK6</accession>
    <name>T1GLK6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02027074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLK6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004413-RA">
        <property type="protein sequence ID" value="MESCA004413-PA"/>
        <property type="gene ID" value="MESCA004413"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1058815_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022048">
        <property type="entry name" value="Envelope_fusion-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12259">
        <property type="entry name" value="Baculo_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004413-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="263" mass="30593" checksum="C253C600A01C30B4" modified="2013-10-16" version="1">MENSVRFVDSRNRSQHQPISFSNAKNNLIFNFNLKKVSNSVAVIQVQMVNPFPKRGELFNVVYIPVRNLGKQGVRYLSEQYIGYVFKFKTEYMLVKSLDKCKYTMDVFYCDEHNVEIVSSFSYDCLVGILKNNRADCNYFEIQSGFKNIVHFGKGNFYYIHQTEIRFSYECPNSIHKGVLVGSGIFTIDENCKFTTNIYSISNNGGEIEQRFFDNKFDEYKVSFLGSLLFISSTSVIILSMLTLCIMSIIKIFRKPRYTMLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLM2</accession>
    <name>T1GLM2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLM2"/>
    <dbReference type="STRING" id="36166.T1GLM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004432-RA">
        <property type="protein sequence ID" value="MESCA004432-PA"/>
        <property type="gene ID" value="MESCA004432"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025092_0_0_1"/>
    <dbReference type="OMA" id="AYTETFA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04450">
        <property type="entry name" value="DEP_RGS7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047016">
        <property type="entry name" value="RGS6/7/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047017">
        <property type="entry name" value="RGS6/7/9/11_DHEX_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040759">
        <property type="entry name" value="RGS_DHEX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746">
        <property type="entry name" value="LP21163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746:SF5">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18148">
        <property type="entry name" value="RGS_DHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="253"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004432-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35971" checksum="3462425355169581" modified="2013-10-16" version="1">MESLVREMQDPENGVPVRQQKMFLTSIPYAFMGYDLIEWLMDHLSIEESEALNIANQLCFHGYFFPVNDTKSLVVKDDSSLYRFQTPYYWPWQHKAPDNVENLKGKWEFISMQAEEQVRLAKERKKGDKIVGDSQERAYWRVHRPPPGQFTPLEPCPVPLRDRQGIKPKKKNVEDYQREVDYLQKSLGRTRMKMSQACESLVTYSETFAEFDFFLHPVLPSNPWVTEDLTFWQLNSAIVDIPTEKRVKRWAISIEELVSDPTGLGEFTTFLEKEYSHENIRFWLAVTKLKRSAQSQVAGRVNEIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM76</accession>
    <name>T1GM76_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rad4 beta-hairpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM76"/>
    <dbReference type="STRING" id="36166.T1GM76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004644-RA">
        <property type="protein sequence ID" value="MESCA004644-PA"/>
        <property type="gene ID" value="MESCA004644"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026889_0_0_1"/>
    <dbReference type="OMA" id="WICIECI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.20.110:FF:000001">
        <property type="entry name" value="DNA repair protein complementing XP-C cells"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2460:FF:000001">
        <property type="entry name" value="DNA repair protein Rad4 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018026">
        <property type="entry name" value="DNA_repair_Rad4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00605">
        <property type="entry name" value="rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-C CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10404">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="88"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="9">
        <location>
            <begin position="142"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="10">
        <location>
            <begin position="205"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004644-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="35202" checksum="F0639A246CFD12E1" modified="2013-10-16" version="1">MPIDLFKGKVNDVGFIQKQLTSPVNYVYGFNSDQTIKDVTARYSKDWANVVRKGRVEPSWLDELLETYVPEPTPKDKREDALMKKAHLEKSLPTSVSDYKNHPLYALERHLLKFESIYPPNPPTLGFVRGEPVYARECVHTLHSREIWLKSARTVKLGETPYKIVKARPKWNKLTNEVIKDQPLEIFGYWQTQEYEPPVAENGIVPRNAFGNVELFKTCMLPIGTVHLQCLKNVCKRLKVDCAPAVIGFDFHGGWSHPVFDGFVICKEFEEKVIDEWNRAEDEYEKKEEERYQERVWGNWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMF8</accession>
    <name>T1GMF8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U5 small nuclear ribonucleoprotein TSSC4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Protein associated with the U5 snRNP, during its maturation and its post-splicing recycling and which is required for spliceosomal tri-snRNP complex assembly in the nucleus. Has a molecular sequestering activity and transiently hinders SNRNP200 binding sites for constitutive splicing factors that intervene later during the assembly of the spliceosome and splicing. Together with its molecular sequestering activity, may also function as a molecular adapter and placeholder, coordinating the assembly of the U5 snRNP and its association with the U4/U6 di-snRNP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TSSC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02130673">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMF8"/>
    <dbReference type="STRING" id="36166.T1GMF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004732-RA">
        <property type="protein sequence ID" value="MESCA004732-PA"/>
        <property type="gene ID" value="MESCA004732"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080817_0_0_1"/>
    <dbReference type="OMA" id="HRNPHKW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029338">
        <property type="entry name" value="TSSC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13445">
        <property type="entry name" value="TUMOR SUPPRESSING SUBTRANSFERABLE CANDIDATE 4 TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13445:SF3">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15264">
        <property type="entry name" value="TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="136"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004732-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="22099" checksum="C4C816B0F582393A" modified="2013-10-16" version="1">MIGKDSVLDQNQSVRSQTFLHTTNKNKVEHLGTKNFRGKKESIFKTPALPISKCLKPRVAPDFQKNPHNWKKYTLSDVDLSSDQTNISAAFAFLKEIEIQKERNSSGENDDKEEEPEAKITFNKCVKIRKNFRNSIGEDSEVDKPKLKGSKLVMPEYVVGQKQKTSKSNRKESSSDSKKPVLNLSHLMDNEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMQ5</accession>
    <name>T1GMQ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMQ5"/>
    <dbReference type="STRING" id="36166.T1GMQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004833-RA">
        <property type="protein sequence ID" value="MESCA004833-PA"/>
        <property type="gene ID" value="MESCA004833"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429597_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004833-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10406" checksum="A9BEBD9DF0269679" modified="2013-10-16" version="1">MSGSSILSSLVNQRETLKGARTKLRTIGNTLGLSNHTMKLIERRFAEDKYILFGGMFLTLVVISIVVYFIVLPDYIRFSFEIKINNNKYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMT2</accession>
    <name>T1GMT2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidoglycan binding-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02073132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMT2"/>
    <dbReference type="STRING" id="36166.T1GMT2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004864-RA">
        <property type="protein sequence ID" value="MESCA004864-PA"/>
        <property type="gene ID" value="MESCA004864"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954211_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004864-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14696" checksum="E3F739B03DD240B2" modified="2013-10-16" version="1">MVFVVLTFASLGYQNSSLHGIAHLSLDCHMATLHAYSSFAYVYSATYLEIPQNQIHVICWNGGIRFVLHFFKFGNIPQTGVIDDATEELIRTPRCGVPDVIKRLSIKVSNRVKRFILQGSKWDHLDLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMT5</accession>
    <name>T1GMT5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMT5"/>
    <dbReference type="STRING" id="36166.T1GMT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004868-RA">
        <property type="protein sequence ID" value="MESCA004868-PA"/>
        <property type="gene ID" value="MESCA004868"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870155_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004868-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7664" checksum="0E4CF977795238AD" modified="2013-10-16" version="1">MHSGSKIDDKDYWDTDDFVPYKEDRHDTFDWKLFREILQHDQSNVIVSPFSVKLVLTLLSEAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMV2</accession>
    <name>T1GMV2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GMV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004886-RA">
        <property type="protein sequence ID" value="MESCA004886-PA"/>
        <property type="gene ID" value="MESCA004886"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_2_1_1"/>
    <dbReference type="OMA" id="QVCEICN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001579">
        <property type="entry name" value="Crooked legs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000253">
        <property type="entry name" value="Crooked legs, isoform H"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004886-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="222" mass="26338" checksum="E27546676432FC0F" modified="2013-10-16" version="1">ETPHRCDFCAKTFTRKEHLLNHVRQHTGESPHRCTYCLKTFTRKEHLVNHVRQHTGETPFKCTYCTKAFTRKDHMVNHIRQHTGESPHKCTYCTKTFTRKEHLTNHVRQHTGESPHKCQYCKKTFTRKEHLTNHIRLHTGDSPHKCEFCQKTFTRKEHLNNHMRQHSSDNPHCCNVCNKPFTRKEHLINHMSRCHTGDRPFACETCGKSFPLKGNLLFHQXP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMX0</accession>
    <name>T1GMX0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMX0"/>
    <dbReference type="STRING" id="36166.T1GMX0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004904-RA">
        <property type="protein sequence ID" value="MESCA004904-PA"/>
        <property type="gene ID" value="MESCA004904"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_628967_0_0_1"/>
    <dbReference type="OMA" id="FICGASE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000140">
        <property type="entry name" value="FiLamiN (Actin binding protein) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="77"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="132"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="222"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="315"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004904-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="436" mass="48245" checksum="67E319DB46BBAB4C" modified="2013-10-16" version="1">MMFSWMPEEEEIPEATWEEYSLYYWNKFKMLTMSEYDQNLKASIYFMEKNITSNDKINKLDFIPQNFLTQYFVRTRQAEVKMPSGKVDQPIIEDNRDGTVSIKYDPKEEGVHELVVKYNGEQVQGSPYKFHVDSIASGHVTAYGPGLVHGISGEPSNFTISTKGAGAGGLSLAVEGPSKADINYHDNKDGTVSVTYLPTAPGEYKVSVRFGDKHIKGSPYFAKVTGEGRKRNQISVGSSSEVTLPGQINDADLRTLNAYIQTPSGLEEPCFLKRMPSGNIGISFTPRETGEHQVSVRRQGKHIPNSPFKVVVGEREVGDAKKVKVSGKALQEGKTHTENTFSVDTRNAGYGGLSLSIEGPSKAEIVCNDKDDGTLNISYKPTEPGFYIVNMKFADHHVEGSPFTVKVTGEGSNRQREKIQRQREAVPLTEVGSHAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN03</accession>
    <name>T1GN03_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004942-RA">
        <property type="protein sequence ID" value="MESCA004942-PA"/>
        <property type="gene ID" value="MESCA004942"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534294_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004942-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20084" checksum="25702399365013B4" modified="2013-10-16" version="1">MKASNSPTSNMIKSSQMLEYGFKKTKRLRWEIIWRGWMNLLLLKKHVSPNAMVGPIRERHQIEVLLAGICRSSNSSQVKASGECDITKYIPLTYSKHDPYYTGSNYRCSNRVYYNTSSPTFYKSSMIFPLDEFIFVLFKYAKLIEVLSYLVNFVSKNEKVGASTPPLTTPTSNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN53</accession>
    <name>T1GN53_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004995-RA">
        <property type="protein sequence ID" value="MESCA004995-PA"/>
        <property type="gene ID" value="MESCA004995"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640972_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004995-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8904" checksum="1581D2EF0D8E6974" modified="2013-10-16" version="1">MMLINTSVEVKQIIVLAIVLANRCLYILNSTMYSKQNPYESPEMGRSYITDGNQCRCKEVYLSKVNGTKRKGRQRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNW0</accession>
    <name>T1GNW0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TMEM104 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02072283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005271-RA">
        <property type="protein sequence ID" value="MESCA005271-PA"/>
        <property type="gene ID" value="MESCA005271"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025541_0_0_1"/>
    <dbReference type="OMA" id="IIVNYCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005271-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="347" mass="39504" checksum="F57077107068E0C1" modified="2013-10-16" version="1">MLSSDNGDNNGLCWEGYPFTRLTVYRMCLVGFVLIMGPFALFNVSKTKYIQMITILCRWAAFGIMIVLALIRLITINDPAELGHPKAFNFGGLPKLFGACVYSFMCHHSLPSLIAPIANKQKIKFLMSMDYLLICLFYIFLAITGAFAFAHIEELYTLNFIPQSSDSSIGKFMEVIDYFLALFPVFTLSASYPVIAITLRNNLQTMFLDMSRIESYNFFLRRLLFPLLAVIPPLINTYFTDSLTDLVGFTGSYAGTGIQYLIPIFLVYFARKTCGDLLGQGIVNKYRSPFSSNWWLIGVFLWSIGCVTLVTINSLKNETDEPRKKILKKSSNLFYEDFSLFFFVYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPS8</accession>
    <name>T1GPS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF3480 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPS8"/>
    <dbReference type="STRING" id="36166.T1GPS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005621-RA">
        <property type="protein sequence ID" value="MESCA005621-PA"/>
        <property type="gene ID" value="MESCA005621"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016038_0_0_1"/>
    <dbReference type="OMA" id="THAINIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.220">
        <property type="entry name" value="Domain of unknown function (DUF3480), N-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.720.10">
        <property type="entry name" value="Smad anchor for receptor activation, Smad-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022557">
        <property type="entry name" value="SARA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024608">
        <property type="entry name" value="SARA-like_SBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037145">
        <property type="entry name" value="SARA_Smad-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46319">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46319:SF3">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11409">
        <property type="entry name" value="SARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11979">
        <property type="entry name" value="SARA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01421">
        <property type="entry name" value="DUF3480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01422">
        <property type="entry name" value="SARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smad anchor for receptor activation-like Smad-binding" evidence="2">
        <location>
            <begin position="88"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Smad anchor for receptor activation-like C-terminal" evidence="1">
        <location>
            <begin position="304"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005621-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="671" mass="76008" checksum="7411D4E065CA3F0A" modified="2013-10-16" version="1">MLTMDLMDIDKVLNDLENAEIENEQSTRPKLEIGKTKPFWVPDNETTICMRCELKSKLEYTQSSNEHRVCGNCWNFLKPSLNEEKKISPNPNNPMEYCSTLSPYQQVEIENNAKTQMEVMVPVSVLKKQHNIESQKNFKKKVTFSDGISPGKQSNFLFKIPQICSYNCSLDYDIISVDNDINLIKRLQEEHLRFFINKNFFVKAKIVKLACCVNETILNLSTEGLHDVGQDEITILLKFDIAECKIPVEIFQHFTEIYENALAGSFIPELGYSLANQSPFLGSSEHGGFLYIRQSFQCIQDIIVPEAPFLIGILIHKQEIPWAKIFPLRLTLRLGAEYRYYPCPHVSIRERPTKLLFSLNHIKGLVIHMEDRKTSVLIPKNRYDSIMKALCDSTDNILSLGGDFSSAADGHLVCIQNLRSDTDELLNYSTQAINIQGQPRKITGANFIVFNGALKSSAGFSAKCCIVEDGLMVQISAEKLLELRKSLENKMDFEIFCGTTNSEEIQKEIVNINWVQDDCKFNIGVVSPIDGMKFDGIPSIRINKINFQKLSLTNQSVTIRCTEVFILSQNSDEVLRIEEINILDLTDKIAVSTISALQNFLDLLLAKGCLKIGLRTSLSKDLVSYEAGSKVKFPPLYMNSLDNELVPVLHKEANNIQGTLVIELIFKIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPZ2</accession>
    <name>T1GPZ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. ADGF subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02150794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPZ2"/>
    <dbReference type="STRING" id="36166.T1GPZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005688-RA">
        <property type="protein sequence ID" value="MESCA005688-PA"/>
        <property type="gene ID" value="MESCA005688"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022829_0_0_1"/>
    <dbReference type="OMA" id="GHRNDIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000017">
        <property type="entry name" value="Adenosine deaminase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006331">
        <property type="entry name" value="ADGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01431">
        <property type="entry name" value="adm_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF39">
        <property type="entry name" value="ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="30"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005688-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="236" mass="26911" checksum="0A209DD019D45DF4" modified="2013-10-16" version="1">MDGTSYTEMDTAAIYKRTFERFRKDYPDFIGSKLIYAPIRNVDNQTINKYIEIAIELKARYPDFFAGFDLVGQEDLGRPLIDFVEPLLSMPKDINFYFHAGETNWNGQSTDENLLDAVLLGTKRIGHGYALYKHPSVLDVIKERNIAIEVNPISNQVLLLVADTRNHPCSYLFANDYPVVISSDDPSFWKAKPLSHDFYIAFLGIANSHADIRMLKQLALNSIIHSAMNGAEREWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQC2</accession>
    <name>T1GQC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02042346">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQC2"/>
    <dbReference type="STRING" id="36166.T1GQC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005826-RA">
        <property type="protein sequence ID" value="MESCA005826-PA"/>
        <property type="gene ID" value="MESCA005826"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044094_2_0_1"/>
    <dbReference type="OMA" id="EMSHNIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08065">
        <property type="entry name" value="MPN_eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000045">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027524">
        <property type="entry name" value="eIF3h"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045810">
        <property type="entry name" value="eIF3h_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19445">
        <property type="entry name" value="eIF3h_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="5">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005826-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="25986" checksum="78B553FA0CF263B6" modified="2013-10-16" version="1">MKMVKHCHEESSNMDLAQGALLGLVVDKRLEITNCFPFPKSGDENMDEEEYQLTMMRRLRRVNVDHFHVGWYQSADVGNFLSLPLLESQFHYQTSIEESVVVIYDTQKSARGFLALKAYRLTPQAIAMYKDGDFTPEALRTLKVGYENMFVEIPVVIKNSPLTNIMMSELSEMMPVNEGNNFLDLGTASVLENNLRCMIDRVDELYQESMKFNKYQQTVAKQETVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQW9</accession>
    <name>T1GQW9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQW9"/>
    <dbReference type="STRING" id="36166.T1GQW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006035-RA">
        <property type="protein sequence ID" value="MESCA006035-PA"/>
        <property type="gene ID" value="MESCA006035"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654146_0_0_1"/>
    <dbReference type="OMA" id="WTNIATR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006035-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="17524" checksum="5C6F63BBB4EA1CC0" modified="2013-10-16" version="1">MSEILNNRPVITWGIIVINDDRNSSYLRAIDEVANNDPQMIFFLVPNNNAERYGTIKKRTCVDRAIPTQVVTSKTANNQRGLMSVATKVAIQINCKLGCAAWMIDLPLNGLMTIGFDIVKSSRDKSMAYGGLVATMDMKINGGGACRPVHACDVEFTKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS30</accession>
    <name>T1GS30_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium uniporter protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The selectivity filter, in which calcium ions are arranged in single file, is composed of two acidic rings separated by one helical turn along the central axis of the channel pore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02052156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS30"/>
    <dbReference type="STRING" id="36166.T1GS30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006478-RA">
        <property type="protein sequence ID" value="MESCA006478-PA"/>
        <property type="gene ID" value="MESCA006478"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056554_0_0_1"/>
    <dbReference type="OMA" id="DDIYVEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF10">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 15" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="17">
        <location>
            <begin position="18"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="237"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006478-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="276" mass="32023" checksum="02DA211D35042999" modified="2013-10-16" version="1">MKPVSHNVGDFINMLKLEDKGIDRAVIFNKDGIRIASTCSIESLLDEPFCIHINNSKYNVSPPEKQRISSEDLSKLGNVRTLVAQLYEALHVGEYHIRKEKDLIQRLENLKHDITPLEEKRIELGKQALRKTNLKTWLGLGLMSVQFGVLARLTWWEYSWDIMEPVTYFVTYGTAMAAYAYFCITKQVNVTDRQYLLSIHKRAKKNSFDIEEYNSKKRQIAEVEFDLKRLRDPLHLQIPPHFKNSLPPPKRGSSTPSENNEKTESNRKNDEKSVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS64</accession>
    <name>T1GS64_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS64"/>
    <dbReference type="STRING" id="36166.T1GS64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006512-RA">
        <property type="protein sequence ID" value="MESCA006512-PA"/>
        <property type="gene ID" value="MESCA006512"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256783_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="36"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11504" checksum="0345CD7391F065D3" modified="2013-10-16" version="1">HRPKTVGNDHPIAIPTIKHHAPFFDETAELFNQYKDAKLVQIVKIESIGIMIIEGKHKEIGSGIFISDIQEDSGAQQAGLVVGDVILAVNKDILVKVNYDYLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSB6</accession>
    <name>T1GSB6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161525">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA006569-RA">
        <property type="protein sequence ID" value="MESCA006569-PA"/>
        <property type="gene ID" value="MESCA006569"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126756_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588504" evidence="1">
        <location>
            <begin position="17"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006569-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="15033" checksum="63179CB732F6CA20" modified="2013-10-16" version="1">MLFSVIFIAILSFSNAERLGNCKVGHKFENLSMPEFKLSDFENNDPRETEFLRTMVYFKYSEPAFSIWNLPNQITFGYLGSLKKFAIFKSHKDYLEHQNPCSSLENITYFSDTKYVGTVFILESEYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GST5</accession>
    <name>T1GST5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GST5"/>
    <dbReference type="STRING" id="36166.T1GST5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006746-RA">
        <property type="protein sequence ID" value="MESCA006746-PA"/>
        <property type="gene ID" value="MESCA006746"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1733581_0_0_1"/>
    <dbReference type="OMA" id="SGIENHH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006746-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17212" checksum="6F41B5978B8FC812" modified="2013-10-16" version="1">MNLNPENELILTDMSLDGFVDTNEKNGEGRRRQSTSYTLHIVVLPEHKEITLNVDADDTILDIKQKFFYIENISVRHQEWVGWPIGITNSTKLGDSGIENHHTLTVQSNNNKPNRLDKVINLDSDSSADEFEDASEFNADEEFFSDSPPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSU3</accession>
    <name>T1GSU3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSU3"/>
    <dbReference type="STRING" id="36166.T1GSU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006755-RA">
        <property type="protein sequence ID" value="MESCA006755-PA"/>
        <property type="gene ID" value="MESCA006755"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2628954_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006755-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8867" checksum="B3147CABAA19F65F" modified="2013-10-16" version="1">MNQKTTCQKIKDFAVKWLNALDNESPPTFLYGEQYGRGTIDTTWKDEITKICLGVALVLMPYCDDILNDLSKVYTTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT69</accession>
    <name>T1GT69_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02167458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167459">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT69"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006891-RA">
        <property type="protein sequence ID" value="MESCA006891-PA"/>
        <property type="gene ID" value="MESCA006891"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2765150_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577686" description="VWFD domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006891-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7878" checksum="7B44FE8E35ED9942" modified="2013-10-16" version="1">MYLFLRTTLLGLLSCYSVIHSANGSVILESKEHTIFPFKRAILANCTVGNTTYFHGQTFKLDCKTQCVCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT92</accession>
    <name>T1GT92_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT92"/>
    <dbReference type="STRING" id="36166.T1GT92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006917-RA">
        <property type="protein sequence ID" value="MESCA006917-PA"/>
        <property type="gene ID" value="MESCA006917"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2335991_0_0_1"/>
    <dbReference type="OMA" id="GHTNIEH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051883">
        <property type="entry name" value="AQP11/12_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191">
        <property type="entry name" value="AQUAPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191:SF16">
        <property type="entry name" value="AQUAPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006917-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="10622" checksum="37DD7E61E556B622" modified="2013-10-16" version="1">MLCRLASKSLSEIGPRFGNVIDSFIGTSLVVAAFNFSGGYFNPVLATALKWGCSGHTNIEHIIVYWIGACVGAVLSVPVFKMNIVRKYLIGDQKGKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTW0</accession>
    <name>T1GTW0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-1,4-N-acetylglucosaminyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTW0"/>
    <dbReference type="STRING" id="36166.T1GTW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007154-RA">
        <property type="protein sequence ID" value="MESCA007154-PA"/>
        <property type="gene ID" value="MESCA007154"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576603_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035248">
        <property type="term" value="F:alpha-1,4-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051981">
        <property type="entry name" value="Glycosyltransf_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12042:SF21">
        <property type="entry name" value="ALPHA1,4-GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12042">
        <property type="entry name" value="LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007154-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9769" checksum="AD2D34E6E2EA52B4" modified="2013-10-16" version="1">MYTENTIAEDWIRSEELFSSKYMYSHLSDFLRYMTLWRFGGIFIDTDTVVMKSFENVSDSRMSNTLEVRLWDFQTKDLDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU75</accession>
    <name>T1GU75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02120304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU75"/>
    <dbReference type="STRING" id="36166.T1GU75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007283-RA">
        <property type="protein sequence ID" value="MESCA007283-PA"/>
        <property type="gene ID" value="MESCA007283"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049090_0_0_1"/>
    <dbReference type="OMA" id="ITTMVTY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033041">
        <property type="term" value="F:sweet taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF131">
        <property type="entry name" value="GUSTATORY AND ODORANT RECEPTOR 63A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007283-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="444" mass="50055" checksum="23376DBE96DFBD49" modified="2013-10-16" version="1">TKPKTVFLDVKPSVNTVTVRKYSDAFDDGVTTRRDSVVLYGTEGRPSLTSAKSLEVFQKDIFFIDIYPITIYLRAIGVLPVTRPGPAQAHFKFLSASFFYSVLFFVGITSYVGYVTVNRLHIISGLKGPFEEAVIAYLFLVNIFPIALVPLLWLETPKIAKVWNDWNDFEILYYQISGSGVPLKLRKKFLTIAIVLPILSVVSVVITHITMSDFNLIQVIPYCLLDNLTVMLGAYWFACCECLSATAYILAERFQKALRHIGPAGMVADYRAMWLRLSKLCRDTGTATCYTLTFMALYLFFIITLSIYGLMSHYLQDLDIGLALTAVSNICLLFFICDEAHYASHNVRTNFQKKLLMVELNWMNSDAQTEINMFLRATEMNPSNINCGGFFDVNRNLFKGLLTTMVTYLVVLLQFQISIPDSGKAKVDNITMSMSDMSTMKPIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUI2</accession>
    <name>T1GUI2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUI2"/>
    <dbReference type="STRING" id="36166.T1GUI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007396-RA">
        <property type="protein sequence ID" value="MESCA007396-PA"/>
        <property type="gene ID" value="MESCA007396"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1306125_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF8">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007396-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="211" mass="24376" checksum="8732AC5B5EA01D26" modified="2013-10-16" version="1">MDATCLTLGSLWTFKYLYIFPDKNVKIIIFIILLIGWIKLKVTLISIYCVYDRNGRSFVNGTLKEGQNKVKYQKTWDHRIRFLFGCVTSKKTSKISDISRIFGQYFIDLDVVPSDIVAGLKLLRKYQIREMEESIENKIVISIRGSLSMADAVTDLRGTNEVLPLKVARNDWLAHKDHMERMEPKLPPRRDYSNKLAGDRGRGRPVSDGWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUW2</accession>
    <name>T1GUW2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007534-RA">
        <property type="protein sequence ID" value="MESCA007534-PA"/>
        <property type="gene ID" value="MESCA007534"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120656_0_0_1"/>
    <dbReference type="OMA" id="KAYSAHW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007614">
        <property type="entry name" value="Retinin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931:SF3">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04527">
        <property type="entry name" value="Retinin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577650" evidence="1">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007534-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="12704" checksum="6090A9A768F6BA2F" modified="2013-10-16" version="1">MFKIIAFFAVLAVAAAKPGFLHEPLVSTYAAAPVVSTIVKSVPTSVSHSSSSVVHSSAHVAEPIIAPVVKTTLTAAPVIKSYATPIAHTYSAPIVKAYSAHWYTLHPFHTMQLQLLTALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVG0</accession>
    <name>T1GVG0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVG0"/>
    <dbReference type="STRING" id="36166.T1GVG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007759-RA">
        <property type="protein sequence ID" value="MESCA007759-PA"/>
        <property type="gene ID" value="MESCA007759"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055524_12_0_1"/>
    <dbReference type="OMA" id="DSCCKTR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03155">
        <property type="entry name" value="CD151_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF544">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007759-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19631" checksum="2686A38848B890B6" modified="2013-10-16" version="1">MRDGESEYTLLLGLVFLLELFVGGLAYFYESQIQYEIQEDLNSTFLTSYGIDEERTLAIDLMQIEYKCCGAIRFEDWRASSWFRQNDTIAPIIRLHGERLVPDSCCITITENCGKRDHPSNIPYTGCIYRFTEELRQQLVIIGAIGLGICSMNIIGMILACCLFAKLSELKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVP8</accession>
    <name>T1GVP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02012749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVP8"/>
    <dbReference type="STRING" id="36166.T1GVP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007861-RA">
        <property type="protein sequence ID" value="MESCA007861-PA"/>
        <property type="gene ID" value="MESCA007861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075666_2_0_1"/>
    <dbReference type="OMA" id="HAFSEGT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000016">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="13 14" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="16">
        <location>
            <begin position="21"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15358" checksum="6601D4BFB09DB406" modified="2013-10-16" version="1">MAPKTSGKAVKKSGKAQKNISKGDKEKKRKSRRKESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSNTGDSICFVWLGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVY8</accession>
    <name>T1GVY8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007956-RA">
        <property type="protein sequence ID" value="MESCA007956-PA"/>
        <property type="gene ID" value="MESCA007956"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309209_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007956-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11450" checksum="651A52EBF3FE44A7" modified="2013-10-16" version="1">MEMALLYDGVLLFAESCKSIEYFPIQLDCSNSVWDKGSTYKNYLALLNNTRKYIELCESSPKASNMITNAARYWDMLKTSMSLKGQNLMLRTISSKSKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW85</accession>
    <name>T1GW85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cortactin-binding protein-2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW85"/>
    <dbReference type="STRING" id="36166.T1GW85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008057-RA">
        <property type="protein sequence ID" value="MESCA008057-PA"/>
        <property type="gene ID" value="MESCA008057"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019330_1_0_1"/>
    <dbReference type="OMA" id="HEMRLMN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050719">
        <property type="entry name" value="Cortactin-Actin_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019131">
        <property type="entry name" value="Cortactin-binding_p2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166:SF5">
        <property type="entry name" value="CTTNBP2 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166">
        <property type="entry name" value="FILAMIN/GPBP-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09727">
        <property type="entry name" value="CortBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cortactin-binding protein-2 N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="314"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="107"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008057-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="360" mass="41020" checksum="AC04613B071459FF" modified="2013-10-16" version="1">MPYNQLLQMVSRLEGELQARDVVIACLRNERVKSLLNHIRTRPEVPPSDPFAALFRDKLAIDGNFCNRDTSSLAVQAENEANLIINEQIEILYDMVTRQRQTHNRMVHILTESLDNNQRMRQELEEEKRKHEHDTAQGDDITYGLEIERTKLKQELEAERNQKKKLEKDMKKLQETLEFERNREKQMVLYLMAERKAIIKQYLEEGKRSQDLAQILNEEKQRSDTIAKVASFDTERKQLKLNSAKDDKRIKELEGEVLLLRNELDTYRKKLQQFQPSTGTKPRDEGVGMANVAKVVQPTATVSSVPVSGPTTGIARSLSVAPGVQPHPPASAVSRLNAQQANSGSQSPTVRNPNVSPPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWB1</accession>
    <name>T1GWB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWB1"/>
    <dbReference type="STRING" id="36166.T1GWB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008084-RA">
        <property type="protein sequence ID" value="MESCA008084-PA"/>
        <property type="gene ID" value="MESCA008084"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867437_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008084-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15265" checksum="5D56C04D42C16C2D" modified="2013-10-16" version="1">MASNTTYAMPVVFGSLFINVPCHPIDPTFSREAIAYSWSKTKPKVIFCDGSVYEIVKEVLKDLDLSSEVYILNNHIEGAKKVQDLFVDRGLKERFFQPREIENGDQTAVVLCSSGSTGLSKAVTLSHKSITRMFSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWK5</accession>
    <name>T1GWK5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CLASP N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
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</entry>
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    <accession>T1GXM0</accession>
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    </protein>
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                <person name="Hughes D."/>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <property type="gene ID" value="MESCA008573"/>
    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
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</entry>
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    <name>T1GY21_MEGSC</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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        <name type="common">Humpbacked fly</name>
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        <lineage>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA008741"/>
    </dbReference>
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            <dbReference type="EnsemblMetazoa" id="MESCA008741-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7353" checksum="128359CE1758CA2B" modified="2013-10-16" version="1">MKNDDEKRFFKFSWNRRFLVNYGVNYLELGELLIESRRISKSLDSKCYSILEVEKLSLAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU8</accession>
    <name>T1GYU8_MEGSC</name>
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        <recommendedName>
            <fullName evidence="2">Ras-associating domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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                <person name="Hughes D."/>
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            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
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        <property type="gene ID" value="MESCA009035"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="233"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009035-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="302" mass="33791" checksum="4686E495B59C5A1C" modified="2013-10-16" version="1">MDFEEFKKSVHLEYVNGANNFLEENSGSLKNNQRIDPSRINDSLKLYGGENPLSKSFNEKALRTIDPTFITDTLNLKGGGGYNLQKSSSTSTTSSSTYQHNKDVFEKGLNRSKSGPNCYVDDSDKDGDGTLKPGTVKRNGNSVGGRYVNIKMDCYNELENDESLSKVTDSNTLDAAGELTTDTPSFGIESSKPFITEEGVVLRRPPKSGSAAIKRRSGNKRTRVKLKRRCSINGHFYNRETSFFKPPYGSQMSVWVTSLVNAQDVINLILEKYKVDSRPEKFSLFVIKDNGEQKRLKDDEYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZC4</accession>
    <name>T1GZC4_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZC4"/>
    <dbReference type="STRING" id="36166.T1GZC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009223-RA">
        <property type="protein sequence ID" value="MESCA009223-PA"/>
        <property type="gene ID" value="MESCA009223"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673942_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009223-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8325" checksum="DB5C406496EDDF8D" modified="2013-10-16" version="1">MNDTRIQDLMTEKGTIHTKRHSGEDPIKMGTCKSDSGIRAFTQNIRPSRGADKIRAASGPSILPRPEDIIFNESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZZ5</accession>
    <name>T1GZZ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02029453">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009457-RA">
        <property type="protein sequence ID" value="MESCA009457-PA"/>
        <property type="gene ID" value="MESCA009457"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280606_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009457-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11555" checksum="CF8D0725C1DAEF30" modified="2013-10-16" version="1">MGWTCAGNGRECPGKNNVSLQTGRDIKRKKGRPRTKWSDVSYTQLANNSKKPCNMEGSKDQAESNSRLVISEKVQKLQYCLSSRMINVVNPWSNSVRRRVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZZ7</accession>
    <name>T1GZZ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZZ7"/>
    <dbReference type="STRING" id="36166.T1GZZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009459-RA">
        <property type="protein sequence ID" value="MESCA009459-PA"/>
        <property type="gene ID" value="MESCA009459"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873113_0_0_1"/>
    <dbReference type="OMA" id="TISHKYW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000025">
        <property type="entry name" value="luciferin 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577774" description="AMP-dependent synthetase/ligase domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009459-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="35226" checksum="CF8B71B6E8C897E1" modified="2013-10-16" version="1">MASNTTYLMPVLFGSLFLNVPCHPIDPTFSKEAIEHSWSKTKPKIIFCEGKLVQMVKKAIFDTGLKCDIYTLNGEVTGIKKVEDLFVAKTNDESNFQPLEIESGDQTAIVLCSSGSTGLSKAVTISHKYWTQLFSIFTDTDQDIVLTFSSLYWLSGLMGFLNAGITGATHLLISEPFHPDLALHLIDKYKVTKTLLPPRNIALILNSPEIGKKSLKSLRTVTCGGARLPPEIRQRFKQQLVPNCLLLFGYGMTEKGLIAAAFIEEKVDSTGVVGFNTEIKIVDEEGNNLGVGEDGEIMVRNPVKWSGYYGDQEATDEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0J1</accession>
    <name>T1H0J1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0J1"/>
    <dbReference type="STRING" id="36166.T1H0J1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009670-RA">
        <property type="protein sequence ID" value="MESCA009670-PA"/>
        <property type="gene ID" value="MESCA009670"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001357_4_3_1"/>
    <dbReference type="OMA" id="RESHENT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001046">
        <property type="term" value="P:positive regulation of integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900026">
        <property type="term" value="P:positive regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09334">
        <property type="entry name" value="LIM4_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09335">
        <property type="entry name" value="LIM5_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000011">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000092">
        <property type="entry name" value="LIM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017351">
        <property type="entry name" value="PINCH-1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210:SF0">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="64"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009670-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16420" checksum="AAD72B0D5671F720" modified="2013-10-16" version="1">MGVPICGACRRPIEERVVTALGKHWHVEHFVCAKCEKPFLGHRHYENRGLAYCETHYHQLFGNLCFICNQVCAGDVFTALNKAWCVEHFSCTVCDQKLTQKSKFYEYDEKPVCKKCYEKFPSELRQRLRESHENTMKKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H187</accession>
    <name>T1H187_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H187"/>
    <dbReference type="STRING" id="36166.T1H187"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009941-RA">
        <property type="protein sequence ID" value="MESCA009941-PA"/>
        <property type="gene ID" value="MESCA009941"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329233_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009941-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23484" checksum="B6F8DDEFE0809861" modified="2013-10-16" version="1">NFQLKKSKSRCKSANNTNNINSGNISNPTNINSKKMKEMKRIRTPLDMPTTDPGSDLLFSTDDEYFFDDGYGTVPRVRKTLLQSELQRKYIDESGIFEKLYQRAPPELLVTPAKHSQQKLNVQSLAPIQQEDSAMNSLNNSSKKFMGPNSPEFPPIIGSVIARRNNSWHNLNNKCVPKIRVQNMNSNTGTESMFPKTDNMATRITRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1C1</accession>
    <name>T1H1C1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1C1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009980-RA">
        <property type="protein sequence ID" value="MESCA009980-PA"/>
        <property type="gene ID" value="MESCA009980"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3413310_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009980-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="28" mass="3252" checksum="B12988A279766257" modified="2013-10-16" version="1">MNSTDMSIQWPHTPTWLNLLLYSSSQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1L0</accession>
    <name>T1H1L0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1L0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010073-RA">
        <property type="protein sequence ID" value="MESCA010073-PA"/>
        <property type="gene ID" value="MESCA010073"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929959_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010073-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="13868" checksum="A746AF44218E24DA" modified="2013-10-16" version="1">MDCGAAIGVYNELPLLQLATTTIPSKISLENSFNKLNGSSVCQEESTVEGPLINIVQPPLSLHKHEIVGTLGNLGDGSSLSQELSRKGKVELCSTPLTEPGMGTSCLIFHAVTVRAASNFLVPVKGPCRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1P6</accession>
    <name>T1H1P6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Potent anticoagulant protein that inhibits the hydrolytic activities of all serine proteases tested (trypsin, thrombin, elastase, and chymotrypsin), with the highest efficacy on thrombin.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02038767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1P6"/>
    <dbReference type="STRING" id="36166.T1H1P6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010113-RA">
        <property type="protein sequence ID" value="MESCA010113-PA"/>
        <property type="gene ID" value="MESCA010113"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164133_0_3_1"/>
    <dbReference type="OMA" id="MRCFAIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000021">
        <property type="entry name" value="Serine protease inhibitor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF374">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1203">Blood coagulation cascade inhibiting toxin</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588673" description="BPTI/Kunitz inhibitor domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="8">
        <location>
            <begin position="25"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010113-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8720" checksum="9D7038FEB38FDEDA" modified="2013-10-16" version="1">MKLSFCIATFIALFSVVKASVPEICKLPSKFGLCKALIPRYFFNINLNKCEHFTYGGCGGNANNFQSEDECNSACNINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1X1</accession>
    <name>T1H1X1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1X1"/>
    <dbReference type="STRING" id="36166.T1H1X1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010194-RA">
        <property type="protein sequence ID" value="MESCA010194-PA"/>
        <property type="gene ID" value="MESCA010194"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1191064_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010194-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="233" mass="26870" checksum="D21E44D9D732A8CC" modified="2013-10-16" version="1">MKTRKIKTFRKSPKESQRNLLNLEENLPININVDPKKTLFSKMKNAFSSIADQKLGLSPKGGNPRKTQIDIYYFDRGNAEFLRTTDGTPAVYSESLLNQTEYYANRVWAEIFGSLQIGVTFFISFVLQLYRFILYSLFRVLIVGFLQITSDYCLKPIMTVLFNGFLQPPLIFLNIYEGIKWQGTYNLKSADPEGIFPALIQQSLDDLLEIRMLMLFTKPCLNHNEISTNILPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1X7</accession>
    <name>T1H1X7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02185669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H1X7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010201-RA">
        <property type="protein sequence ID" value="MESCA010201-PA"/>
        <property type="gene ID" value="MESCA010201"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_467934_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="6">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="6">
        <location>
            <begin position="278"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010201-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="583" mass="67621" checksum="3F72617311FA7FF9" modified="2013-10-16" version="1">MQQDAHEFLNFLINHINEIILAERLQGNKNLKHNSSTNVSTTSNITISSTTISTNSTPVEPTWVHEIFQGILTSETRCLNCETVSSKDENFFDLQVDVDQNTSITHCLRCFSNTETLCSDNKFKCDNCCSYQEAQKRMRVKKLPMILALHLKRFKYMEQYNRHIKVSHRVVFPLELRLFNTSDDAVNPDRLYDLSAVVIHCGSGPNRGHYISIVKSHGLWLLFDDDMVDXYLLWRKQIYLRRNVIKVRNLLFFKMGANISQLEREIGIEQFPPNEHYFGLVNFGNTCYSNSVLQALYFCKPFRDKILEYRAKNKRPKETLLTCLADLFYNIATQKKKVGSIAPKKFIARLRKEKEDQRLNTSECGSSEKPVSSGYRGSSTLLNDSELNVGDDIYIENKNENIITPRTLKSILRNHKNGDKRNSMFENKKNNQMFVELKHYEEIAHILNSNDEHYIEYDSMNLKREVNKASQKKVSFTENLRKHFVTPPNSPNISMSNFKVLSSKKKQKSFESNKNMIPDTSKLGKISSNRFKRLQIQWELLSKESSMLNEFCNTNEYDSNKIIPANPLPKSKIPRPVSYPVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2P2</accession>
    <name>T1H2P2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pupal cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2P2"/>
    <dbReference type="STRING" id="36166.T1H2P2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010495-RA">
        <property type="protein sequence ID" value="MESCA010495-PA"/>
        <property type="gene ID" value="MESCA010495"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375272_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF238">
        <property type="entry name" value="CUTICULAR PROTEIN 65EA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010495-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="21356" checksum="0BC0577C42CFDBC2" modified="2013-10-16" version="1">AFRTAPNSYKSKGFKTTPVLYKQTSSSSGSDVPVLNMVSDIQADGHYNYEYQLGNGIAASEKGLGGQNVQGSFQYTSPEGEPISITYTADENGYHPHGSHVPEIPSYILKSLEYIRTHAPYDESKHAAASTKSFVAAPARAAYKAPAVKSVPVFKPAKAPAKSAYRSAPVKTSTAFKKTPVSASRAYLKSNQKNGRLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3G6</accession>
    <name>T1H3G6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Translocon Sec61/SecY plug domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3G6"/>
    <dbReference type="STRING" id="36166.T1H3G6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010793-RA">
        <property type="protein sequence ID" value="MESCA010793-PA"/>
        <property type="gene ID" value="MESCA010793"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_0_0_1"/>
    <dbReference type="OMA" id="LEVTIIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000007">
        <property type="entry name" value="Sec61 translocon alpha 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="12">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010793-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="296" mass="32598" checksum="BFBB29531F74E566" modified="2013-10-16" version="1">MGIKLLEVIRPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPLYWLRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMVITIGQAIVYVMTGMYGDPSEIGAGVCLLIIIQLFAAGLIVVLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVSTGRGTEFEGAVIALFHLMATRNDKVRALREAFYRQNLPNLMNLLATVLVFAVVIYFQGFRVDLPIKSARYRGNTAVIQSNFSIHQIFQLSCNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3U1</accession>
    <name>T1H3U1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3U1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010929-RA">
        <property type="protein sequence ID" value="MESCA010929-PA"/>
        <property type="gene ID" value="MESCA010929"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443347_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010929-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10443" checksum="6BBD6ED14DE3A1A1" modified="2013-10-16" version="1">MLKILRQNPANNNTASRYHLQGIQYKNFTQSPKWQIQGTKIKKNDLVIIEEPNIFSHKWPIGVVHPGIGGLKQADRSKLEPFKLQEMTQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Y7</accession>
    <name>T1H3Y7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02124029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Y7"/>
    <dbReference type="STRING" id="36166.T1H3Y7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010984-RA">
        <property type="protein sequence ID" value="MESCA010984-PA"/>
        <property type="gene ID" value="MESCA010984"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_971707_0_0_1"/>
    <dbReference type="OMA" id="HYVETID"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000008">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF290">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="16">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="15">
        <location>
            <begin position="88"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010984-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="287" mass="33591" checksum="2BDD07ECF163CCF6" modified="2013-10-16" version="1">VHKEIGTYSLTIKYNGTLRDDKLGFYRSNYTNAKGETVWLAATQFAPTYARSAFPCLDEPNKKATFDIKLTYPADKFAISNMPNSSFVVKIDADISIRPMTHYVETIDEIKKNYDKIAYEKSSCVLRMFDNAIGKDKFKKAVDMYLQNKSFKNAEEDDLFKYLEEAFKIYGDNSVNITKAMKSWTNQRGFPIVFANVNYREGTIELSQQRFVDKFQKDLDDPIYFIPINYISSKTERPKNHTAFTWFKNKTQTYQLPNEFSVNDWIVLNFEQTGYYRINYDNSTWSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Z4</accession>
    <name>T1H3Z4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Z4"/>
    <dbReference type="STRING" id="36166.T1H3Z4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010991-RA">
        <property type="protein sequence ID" value="MESCA010991-PA"/>
        <property type="gene ID" value="MESCA010991"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148703_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010991-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12670" checksum="E9D3CDE0B05D973C" modified="2013-10-16" version="1">MQKHQTRQNPSPAYSDEGLGTKKTIFVLCVVVGCIGILWPKLFYPMFFGNSSPPLPQNRGSVKKLGKCCDVVLDKDKFFNSTLAASSITFGPDLFRRHDKMYQDEISMTFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4A5</accession>
    <name>T1H4A5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4A5"/>
    <dbReference type="STRING" id="36166.T1H4A5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011113-RA">
        <property type="protein sequence ID" value="MESCA011113-PA"/>
        <property type="gene ID" value="MESCA011113"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500469_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011113-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9478" checksum="738C71EE70A0C60F" modified="2013-10-16" version="1">MVTYFLKDKLKECQTVNQVDDVVSNSTAQRRKSLCRQHNISSSFGTTCSATNTPMSPSSRSNSSISILNTTTSCQKTNNNNNNNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4E4</accession>
    <name>T1H4E4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387697">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4E4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011153-RA">
        <property type="protein sequence ID" value="MESCA011153-PA"/>
        <property type="gene ID" value="MESCA011153"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624806_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011153-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9364" checksum="2AD8AE620B57C381" modified="2013-10-16" version="1">MKGKILEDKSTAKRFFCELCKKGFKQNSLYQKHLKNVHCLICAKSFPTKMRYEQHIQTIGHKNLIFNKEYQEKLKQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4H3</accession>
    <name>T1H4H3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4H3"/>
    <dbReference type="STRING" id="36166.T1H4H3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011182-RA">
        <property type="protein sequence ID" value="MESCA011182-PA"/>
        <property type="gene ID" value="MESCA011182"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712027_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF246">
        <property type="entry name" value="ROUNDABOUT 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011182-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8124" checksum="2557F4561B7A6DAE" modified="2013-10-16" version="1">ESAIAILKVHIKPFIIRGPHDQIVLEGSSVTFQCRVGGDPMPDVLWMRTASGGNMPLDRVQILEDRSLRLDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4I8</accession>
    <name>T1H4I8_MEGSC</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4I8"/>
    <dbReference type="STRING" id="36166.T1H4I8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011197-RA">
        <property type="protein sequence ID" value="MESCA011197-PA"/>
        <property type="gene ID" value="MESCA011197"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2283962_0_0_1"/>
    <dbReference type="OMA" id="RTFWRES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF415">
        <property type="entry name" value="FI20190P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011197-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12628" checksum="E1E0805DF3624773" modified="2013-10-16" version="1">MDHSKKPEKENPKKKSNPLSQLFFTWTIPLLCKGAKKGLNQDDLTKCMEKDRSEELGDRLEDEWYKEIDRAQKKGREPKLKNALFRTFWRESFFDGILYAIFITIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4X8</accession>
    <name>T1H4X8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383339">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4X8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011349-RA">
        <property type="protein sequence ID" value="MESCA011349-PA"/>
        <property type="gene ID" value="MESCA011349"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3280062_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011349-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="4757" checksum="1D194326E128DFFD" modified="2013-10-16" version="1">MCLRSTGTDSGKRFPIIFPKTSIWYLLLNRSHYPTRCILGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1H588</accession>
    <name>T1H588_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H588"/>
    <dbReference type="STRING" id="36166.T1H588"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011463-RA">
        <property type="protein sequence ID" value="MESCA011463-PA"/>
        <property type="gene ID" value="MESCA011463"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834081_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011463-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7475" checksum="E60D9D19912663EE" modified="2013-10-16" version="1">MEPNSLERVHEFDQNQQRRLNGISEKNVSNYLSTQVTNMFSKGRSFTSVAFPEAGVQRICAIATIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5D6</accession>
    <name>T1H5D6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5D6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011513-RA">
        <property type="protein sequence ID" value="MESCA011513-PA"/>
        <property type="gene ID" value="MESCA011513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307598_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="211" mass="24639" checksum="81C281FF25192AFB" modified="2013-10-16" version="1">MGLLKADFKDIADVFMKLPKSNQDYILNKLFSIMEKDNFEYDVFYSDSYSIENAKKMFNDKLFVLFQISEEKLSSVPNYRLNPEFADNDNSAFEWLSEYEMSFIYPTEDTPDITTPIYTPDEEESTFSAIFVDYDDKTETNILETTISEDLENDVEIFTTTESPDEDYSEEDEEGQQEEEEEVEEFLPSGADDVDEEENDNELNLPNLVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6B5</accession>
    <name>T1H6B5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium-activated potassium channel BK alpha subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H6B5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012248-RA">
        <property type="protein sequence ID" value="MESCA012248-PA"/>
        <property type="gene ID" value="MESCA012248"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485617_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcium-activated potassium channel BK alpha subunit" evidence="11">
        <location>
            <begin position="146"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012248-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="20512" checksum="2EBBBB1949E63B52" modified="2013-10-16" version="1">MRVISIKNYSDDIRVIIQLMQYHNKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSPDTQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6H7</accession>
    <name>T1H6H7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6H7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012310-RA">
        <property type="protein sequence ID" value="MESCA012310-PA"/>
        <property type="gene ID" value="MESCA012310"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_456801_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052586">
        <property type="entry name" value="ASCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494:SF0">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2 ASC-1 COMPLEX SUBUNIT P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012310-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="598" mass="68330" checksum="DBF3BBE2723A767B" modified="2013-10-16" version="1">FKFQDCRSISSYTSLVKPGGQGKSGIDLEEWKYQASRFLYNIQYLLQLEHHKFWSYIILEKSVMASIASFLQECTPFYLDKNISDNIAKTLYNDILRDVVRVFCRIITNKEAENKWISLTDLSNLIYSNYLITVPMLMDLILAVAKETIRLLKIFESCIEIQPNYERDLRCFEKLSQAMQILSKKEDLENSASGAMLDTPYDEVVLFSLDCAYSLSILLDIWPDMRQCGAIHRFYDCTIPNLWRNIHVINPYAIALNWLNHTRIQFLRCFRAIIIISEEVLIDPDKSLEPAEEIISIFTESLADKTFVIDYERMYPIGVDFEILKQACDKLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6N0</accession>
    <name>T1H6N0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6N0"/>
    <dbReference type="MEROPS" id="M10.036"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012363-RA">
        <property type="protein sequence ID" value="MESCA012363-PA"/>
        <property type="gene ID" value="MESCA012363"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923100_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF291">
        <property type="entry name" value="MATRIX METALLOPROTEINASE 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="206"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012363-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="302" mass="35303" checksum="7C02968C42082521" modified="2013-10-16" version="1">NQTMRVPNSGMVRRVLTNAFQVWSHNSKLNFREVYSRDADLQIVFAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMKLMEVAVHEIGHALGLGHSTVEGAIMYAWYHKYKIEGNKLPDDDRLAIQQLYGARHPVWAPYNPPARTLPPPTTTTTTTMRPLIYTLQYQYNQHMQWSNALFDNRCRTRPQQHLELTTTTPTQRRPNRPRQRYPHRTYPRREKPDQCNMSYDAITIIRGELIFCEGKLFNGFPFEIRRLWKDLPRDFTHVDAVYENEKQEIVVFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9W7</accession>
    <name>T1G9W7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9W7"/>
    <dbReference type="STRING" id="36166.T1G9W7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000008-RA">
        <property type="protein sequence ID" value="MESCA000008-PA"/>
        <property type="gene ID" value="MESCA000008"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123885_0_0_1"/>
    <dbReference type="OMA" id="MDKMNDY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026182">
        <property type="entry name" value="ANAPC15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22526">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX C SUBUNIT 15, PSEUDOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22526:SF2">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX C SUBUNIT 15, PSEUDOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15243">
        <property type="entry name" value="ANAPC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000008-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="13238" checksum="B2B2801F06BA7D0D" modified="2013-10-16" version="1">MFMNSMFPSLEPRFANHVFFELDPVNNEESEIQKFEKTQENWLHSISNLSMDLRPFGKLEPQQGQETDDEDVNDESEDETDDANEEEDMVLEPNDLQITYSPTTEMAANETNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9X5</accession>
    <name>T1G9X5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9X5"/>
    <dbReference type="STRING" id="36166.T1G9X5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000017-RA">
        <property type="protein sequence ID" value="MESCA000017-PA"/>
        <property type="gene ID" value="MESCA000017"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019484_4_1_1"/>
    <dbReference type="OMA" id="GNEYSVF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22547">
        <property type="entry name" value="SP6-9-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000077">
        <property type="entry name" value="Sp8 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF15">
        <property type="entry name" value="SP1, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="386"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000017-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="477" mass="51337" checksum="15AB08A810A92AF9" modified="2013-10-16" version="1">MLAAQCNKLSSKSPPPLADAAVGKGFHPWKKSPGSVIPGQHSPCGNISSSSGSPSLSSTTGNNLQQQHQSNSGVQKSQPSSTMNNISARSITSSASSCSGVTSPLGSYNSDLYFPSATTSPSNNDNSPASAAHMHQSLLGKVDSAAFGTMYSRHPYDWPFNAVTATHKTEGVNTGWWDMHTAAGSWLDMGSAAGMHSTMANYATGGTDYSSLTHSLSNTAHLLGSGQHLLQDTYKSILPGQGNIGNFTLPHSTPVSSSGTVTSPSTPSPRSQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICNKRFMRSDHLAKHVKTHNGATNNNNNNNNNNNIKKGSSESCSNLHHQNSQQNSHPGTPNHMASHMASHHQMGGSPPIMQQQQQHYHNSSPGLIDHHSNLVDVKPPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA15</accession>
    <name>T1GA15_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM122 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02117081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02117082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA000058-RA">
        <property type="protein sequence ID" value="MESCA000058-PA"/>
        <property type="gene ID" value="MESCA000058"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098792_0_0_1"/>
    <dbReference type="OMA" id="WSCKSAD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026716">
        <property type="entry name" value="PBIR1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22227">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 122B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22227:SF6">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 122B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000058-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="26962" checksum="C43E25D1CA343785" modified="2013-10-16" version="1">MEVDSSSTTTNSSKLKRCISVPAISSKSGPKIVPTTPEDQKYDETPKKMDVPLPCSSNSRPSSRSLKGXIFSTQRARRYSASFSPLSANGASGPRMSLRVSQLRQEECADVNSREQNHEREVHSAMQISQSWEDLTLVAENWSCKSADDVTNPLQVVLPSGILSCSSPSPTSRGSGANSNSSYRALPYGLSPSPTRKTFATRRSMSPIAMRPSQLGPVKRRFDIDGDNCWSNAYSPPPFKKIFVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAR9</accession>
    <name>T1GAR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ERAP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02198881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAR9"/>
    <dbReference type="STRING" id="36166.T1GAR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000329-RA">
        <property type="protein sequence ID" value="MESCA000329-PA"/>
        <property type="gene ID" value="MESCA000329"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1020448_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000008">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3480.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF301">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="15">
        <location>
            <begin position="193"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000329-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="273" mass="31787" checksum="9F603279DE8A0608" modified="2013-10-16" version="1">MLWENIDINSNAHTTRSLHVYFCEDVERVGDDCQVTPVIHVLQYPGCVPKPIPSFACVGRCASYLQRMLCNFSYMKTSTPEKLFKILDKHWPVEDKNKIGDIMEFFKSWTEQVGYPVVNITLAADGLSFSVRQERFLLKEKYIGKTSLRNLTYVIPLTYVTSFENDFQNTDTKEYLPKSNKVVTINLSQKADWIMANTQQIGYYRVFYDNKTMYSIHQALIAPNHSNINENNRAQFIDDLLSFARAGYLNYYETLQMLEYIENEESYIHGLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAS4</accession>
    <name>T1GAS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000334-RA">
        <property type="protein sequence ID" value="MESCA000334-PA"/>
        <property type="gene ID" value="MESCA000334"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1688737_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000334-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="16885" checksum="E868F580E0D6CD80" modified="2013-10-16" version="1">MSPTNFTIKAGKFLNIDTVQFLTSNKTNQTKQNVQTYLSHLRCYRLCRRQTRNCSSFSLLIPSHCSPRSSYATSSQFVARTHNGIVAPVAAKIVAPAPLAYTAPIVRAAYTFPVVASAPIIKAAAPLAYTAPVAYSSPLVHSHLASPLTYSAPLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB05</accession>
    <name>T1GB05_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cuticle protein 64</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB05"/>
    <dbReference type="STRING" id="36166.T1GB05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000424-RA">
        <property type="protein sequence ID" value="MESCA000424-PA"/>
        <property type="gene ID" value="MESCA000424"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827514_0_0_1"/>
    <dbReference type="OMA" id="ANTYRVS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576777" description="Cuticle protein 64" evidence="1">
        <location>
            <begin position="17"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="13820" checksum="1971CB3BCF565384" modified="2013-10-16" version="1">MFKLLILSVLVAAASAGYIGGYGGYGGYGLGYGHGYAAPAIASAPLISHSAPIISHAAPIAVAAPIKAVAAPLATSYANTYRVSKVAAVAPVVHAAPAVVSHALYGHGYGLGLGYGHGLGYGGYGYGGYGHGLGYGGYGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBG3</accession>
    <name>T1GBG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBG3"/>
    <dbReference type="STRING" id="36166.T1GBG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000595-RA">
        <property type="protein sequence ID" value="MESCA000595-PA"/>
        <property type="gene ID" value="MESCA000595"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_12_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000595-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="235" mass="26870" checksum="892F034BE73E437A" modified="2013-10-16" version="1">MKDVLTLHRLDRLTSGLLLLLRIQTKLEKLRNKSGTEKFKKSICAKLKVERIECPEPIEVVSYKIGVCRVSPKGKDCLTTFEKISSDGETSIVLCKPKTGRMHQIRVHLQYLGYPIVNDPLYNHTVFGPTKGKGGDIGDKTNDQLIQELIKIHTAENFIALDLEPDMNNIKEVSKEVPEHDCYECTQTYTDPKPEQLHMFLHAYKYSGKDWSFQTEMPSWAKATLEKTTEENSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBU6</accession>
    <name>T1GBU6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBU6"/>
    <dbReference type="STRING" id="36166.T1GBU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000735-RA">
        <property type="protein sequence ID" value="MESCA000735-PA"/>
        <property type="gene ID" value="MESCA000735"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1961417_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576802" description="Amino acid permease/ SLC12A domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000735-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="13480" checksum="E2C0DF25D7E0EF68" modified="2013-10-16" version="1">MASYKLVVAAIFVICVLNSTTAQQQNTPNESSIVESARTFGRHFLKRISFAIIPAAFVIGAVTTLLAAITVTSMNGLGVGVIMLILTIGQMLSRSSLATSYAAQTQAAPLPLVYGQAAQQPVYLGKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBU7</accession>
    <name>T1GBU7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tiam1/2 second PH-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBU7"/>
    <dbReference type="STRING" id="36166.T1GBU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000736-RA">
        <property type="protein sequence ID" value="MESCA000736-PA"/>
        <property type="gene ID" value="MESCA000736"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_544665_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01255">
        <property type="entry name" value="PH2_Tiam1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055230">
        <property type="entry name" value="PH_Tiam1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043537">
        <property type="entry name" value="Tiam1/Tiam2/Sif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46001:SF3">
        <property type="entry name" value="PROTEIN STILL LIFE, ISOFORM SIF TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46001">
        <property type="entry name" value="TIAM (MAMMALIAN TUMOR INVASION AND METASTASIS FACTOR) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23014">
        <property type="entry name" value="PH_Tiam1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tiam1/2 second PH-like" evidence="3">
        <location>
            <begin position="18"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000736-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="501" mass="56431" checksum="132768B79F6FC350" modified="2013-10-16" version="1">MEKVAEHINEMQRIHEEYGAIFDHLFRQHKNHANNLLTSVQVSDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVTSKSTTNEVEIIRYQVLIPVTEVQVKTSSAKDMESHYLWELVHMKSQIQRRVEKQYVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGSNSSVSSSQTLERPKQQITIMQGSQTLEAEDVPLEPTMHSGFRGRSKTVGDISDPDQQQQQQDSDTGVMMTQSTHTQQRTHIQMRIGDGAEHMDQPDHGIKSEGEEDSQSQMGTFRLKPSLGRTPNHLTLSTTSTISVGSTGSQARLVHSSNTPSNYQPILMKDLGKTTAITTNKSESNNTIISSSCSYKNSVIGSSVSSFKATLQQTLNQSRPEAQLSQMKVSPQQSPDQDIEIIEIFPELPDSNSNENGREPSLSEQTNTCVMDKHLSDLEQDNLNTKIQYDIIKYMKNLREKTLEAKEAQKQVAAAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCE7</accession>
    <name>T1GCE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCE7"/>
    <dbReference type="STRING" id="36166.T1GCE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000958-RA">
        <property type="protein sequence ID" value="MESCA000958-PA"/>
        <property type="gene ID" value="MESCA000958"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_2_0_1"/>
    <dbReference type="OMA" id="NLCPKPM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050264">
        <property type="entry name" value="Bact_CCA-adding_enz_type3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46173">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46173:SF1">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="6">
        <location>
            <begin position="59"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000958-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="34039" checksum="F3E83ECD93D1B9F2" modified="2013-10-16" version="1">MFLGFDGSLYDYFFGYEDLQKRRVVFVGNPDQRIKEDFLRILRYFRFYGRIAEKPNHHDKETIDAITENISGLEKISGERIWSELKKILKGNFALELMEEMLKCGIAKYIGLPESLNLEEMRRVYGTKQDLQSITYLAALLDSSEDVLKLHSRLKLSAFERDLAIFLAENRKFVGTDLNELSDYKKRCILHSNRKDFYEELLKYFGKEEIYREFKDWNIPKFPLNGGILKQNNCPSGKVMGSVMTELKAIWAEDNFETSQKDLLSIHLPNVLNKMKIDEFPSNKKLKTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCN7</accession>
    <name>T1GCN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCN7"/>
    <dbReference type="STRING" id="36166.T1GCN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001057-RA">
        <property type="protein sequence ID" value="MESCA001057-PA"/>
        <property type="gene ID" value="MESCA001057"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656526_0_0_1"/>
    <dbReference type="OMA" id="NDTSAHN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF1">
        <property type="entry name" value="RE15579P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apple" evidence="1">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="1">
        <location>
            <begin position="99"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001057-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="18470" checksum="7345D7D2C8A8D1FA" modified="2013-10-16" version="1">MPAIYGRMAFEKLTDFDFSGTTYYSVKNLTLYECQGWCREEADCQAAAFSFVVNPLSPYQETHCQLQNDTSAHNPTASPQRSTNMYYMVKLQLRSENVCIRPWAFERVPNKIIRGLDNALIYTSTKEACLSACLNEKRFICRSVEYDYNNMKCSLSDTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD44</accession>
    <name>T1GD44_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096916">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD44"/>
    <dbReference type="STRING" id="36166.T1GD44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001225-RA">
        <property type="protein sequence ID" value="MESCA001225-PA"/>
        <property type="gene ID" value="MESCA001225"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1084060_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001225-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="257" mass="29912" checksum="B493369CE4029C75" modified="2013-10-16" version="1">LEEVKSKSSLRAILKKDASRSESNIAESDHHKEKHKKKDASSSEDEFKEDSRKKENPFKSVCTPEMAAEWLKMNIMAYPPIFPGIQMYPPVGNIPYRGGNPFRGGRGRFRGRGRGFYHGDSYYGEDDKYSNGYRGRGRRSRSRTRSKSRSRSRSNPRRYDRDRYRRRSRSRSRSLSRNEPRMSKKCFRVEIWENNEMSGRVNLDLAMGDLGSPLLELFDACKHGDLQKVKRLVNSQRDFVNSRDKEGRKSTCLHFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD57</accession>
    <name>T1GD57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02169917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169921">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD57"/>
    <dbReference type="STRING" id="36166.T1GD57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001238-RA">
        <property type="protein sequence ID" value="MESCA001238-PA"/>
        <property type="gene ID" value="MESCA001238"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_610144_0_0_1"/>
    <dbReference type="OMA" id="LADTECV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF27">
        <property type="entry name" value="FI19426P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="7">
        <location>
            <begin position="17"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001238-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="449" mass="49609" checksum="BC8B8E17A5B64E42" modified="2013-10-16" version="1">MGATKEYETKFINKSLISIKVVTFFIVSGLTALHVLHSTKPLLLGLNFSEYRTITIIAPFVSILGPLIAGPLADNLAAKNPAAFEARRPLVSFGCDSEGAVIFQERCSDEATCRHWKGVAGYVKLSNCSYACQNHTDFEDLYNYWVKGSPAPTTEYSSESGGDYDYGSDGPTDTTASFRRRRAEIAATAASTEDNREQISSEILTQKNKRDVLNKVYVEPPHLCMKSKNSDGEEFIEKCHVYTDDTPSITVSTVLRAATNVENDTHSAEWCNYPLDGFKCAVPEMQARYMKSIKNEECKPMVICQVIDPYDSAGSVLENSQCIKTVGDLDTTLNGYFTIRAIGDIFPLAALTLLNTAIVVAVRETSEGRGEVCRQYIWGAIGYVILFSPLDVLYFSDNVETQQTAALIALILVIVCFLLAAIVLLISPGMPISPPEWWWHTKTGMLFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDN9</accession>
    <name>T1GDN9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDN9"/>
    <dbReference type="STRING" id="36166.T1GDN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001429-RA">
        <property type="protein sequence ID" value="MESCA001429-PA"/>
        <property type="gene ID" value="MESCA001429"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402152_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001429-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10728" checksum="F4A76ECB31AA3FE6" modified="2013-10-16" version="1">MEVVNFLTCFWILMFLMMTSICLILTLDSYSKWKDSPIIVSLDSQFSSVSDIPFPAITICPYKKFNMEKKNMTDFISRFHTIPNLSSSAPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDR7</accession>
    <name>T1GDR7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDR7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001462-RA">
        <property type="protein sequence ID" value="MESCA001462-PA"/>
        <property type="gene ID" value="MESCA001462"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852284_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7532" checksum="71335B4EFE44C8C9" modified="2013-10-16" version="1">MLASYIFISGSATPRLCPFLNQDIYEQLHKTYSTIISFDIKLVIQLDEVTKDQIEHLRSVTLVHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDZ4</accession>
    <name>T1GDZ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDZ4"/>
    <dbReference type="STRING" id="36166.T1GDZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001548-RA">
        <property type="protein sequence ID" value="MESCA001548-PA"/>
        <property type="gene ID" value="MESCA001548"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_4_1_1"/>
    <dbReference type="OMA" id="PWAIFAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001548-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24253" checksum="ED0F3CF8EA03457A" modified="2013-10-16" version="1">MGNMKSPYMKLFAPILGEPGTIPDSLTSFEFLPNNQPMEILAKSLCNERSKFMSFCAYSLFLIGGFNELNLNASLIPEIAKVAPAGSSFRQILHYFQEHLSGKFREWDYGPKKNKEVYGKEEPPEYPVDKIKAPTHFYYSKNDFMSSDVDVEKLAKKMKKEYVRELYLIPDHKFTHLDYLYGLNSKKVVYDRIRDNIVTCGDNKVTNLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE81</accession>
    <name>T1GE81_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390127">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001639-RA">
        <property type="protein sequence ID" value="MESCA001639-PA"/>
        <property type="gene ID" value="MESCA001639"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1210987_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001639-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="229" mass="26667" checksum="0FB83B91BC33C8DB" modified="2013-10-16" version="1">MKRFRMRDVSSNFLGFKKKSSNEYIDGHCIPASLVISEHCIVSWSRKTIQVFDNEIFQVVYKWRDVTSQGYLKHCKVKKVMVYVLLFFLHRWALSVIYVTYKCNLLVNYVSNLLFQCNIPIIYVNYTYFPHPTIHFQTWNHPNANIKRHTSNILDVRSVRDANIDSDNNFVRYPPQRNQPIHSLNFLCNIEPNYLALFGSLSSNPSSIGNLKTGLRCLLVKRPSSVLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GED7</accession>
    <name>T1GED7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104326">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA001699-RA">
        <property type="protein sequence ID" value="MESCA001699-PA"/>
        <property type="gene ID" value="MESCA001699"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1379556_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF84">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="66"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001699-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22914" checksum="1EF64258FB4BC606" modified="2013-10-16" version="1">MMERIWLVFHCTKDDSIDHIPMGLXXXXXXXXXXXXXXXXXXXXXXXYLSWARPSQWYKKQPLCLVRKYFGDKIALYFCWLGFYTEMLVYPSIVGTLCFLYGLASIDSADNTPSKEICNTNGVGNTTLCPLCDQACSYQTLSDSCIFARLTYLFDNPSTVFFAIFMSFWATTFLEVWKRKQSVIIWEWDLHPPPGAKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEF2</accession>
    <name>T1GEF2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEF2"/>
    <dbReference type="STRING" id="36166.T1GEF2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001714-RA">
        <property type="protein sequence ID" value="MESCA001714-PA"/>
        <property type="gene ID" value="MESCA001714"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745149_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047278">
        <property type="entry name" value="DEN5A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46070">
        <property type="entry name" value="PINSTRIPE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46070:SF1">
        <property type="entry name" value="PINSTRIPE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001714-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="17565" checksum="F339E25C351BF8D0" modified="2013-10-16" version="1">MISNDEHVSKEKQLSTSFYDILEKIWNHGLQIKQGSSAIWSHISLYLRTSKNMESSKASYNMKSDIRNILNLTIIKTDSGYAKAWIRLALEKKCLSQYFHTLLSEEFLLKTLYKRSSFLRSEDEREQFLYHVLSLSTVDYNCFTNSYPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEG9</accession>
    <name>T1GEG9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001731-RA">
        <property type="protein sequence ID" value="MESCA001731-PA"/>
        <property type="gene ID" value="MESCA001731"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2099665_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001731-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13700" checksum="D85EC58BD44A0B49" modified="2013-10-16" version="1">MKLPLKYRLVENNSSRYTYDKNEEAFFYSGEQLNPKKEFKRLVFLKISPFPCLSERSHYPLSTLNAGTQYAKRAESNFEILSFKRFDRIRVRSQENRNSAPRIASNSSKLQYSAYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEL6</accession>
    <name>T1GEL6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389638">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001784-RA">
        <property type="protein sequence ID" value="MESCA001784-PA"/>
        <property type="gene ID" value="MESCA001784"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161226_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001784-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="11659" checksum="98EF35AD79E13111" modified="2013-10-16" version="1">MAEHDKNLRSKANKNNPVITAGTGLPRTPLTRPRVSRGGASSRGAKTTNNTRLIDSTGKDSNKTLTLTNLASPPVITAPTPQPPKSTGTIPKNTANVFDNNSNAASRIQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEY1</accession>
    <name>T1GEY1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033489">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GEY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001906-RA">
        <property type="protein sequence ID" value="MESCA001906-PA"/>
        <property type="gene ID" value="MESCA001906"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_239652_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00136">
        <property type="entry name" value="PDZ_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47644">
        <property type="entry name" value="AGAP008221-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47644:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="1122"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="1213"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="1338"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="901"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1045"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="753"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="920"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001906-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1443" mass="162265" checksum="0E28B116557A52E3" modified="2013-10-16" version="1">MPRPISTPLEYHSIIRNVAPSASFNPPYQSPKFYLSQSTDQIPTRVYQQPYPGHPQPVMANMDNIRPKHVSFARSQTLASFDEMASLGLRGSGRLRGSRTNSQERLIGGKKVIYTNPQLIHQPVQQQQSQISLLSQNTNPIHQIQYASHHPTYAAFPHAAYYPHLNYSVIRPNVHHPQPKKSDEYDQSDYVPIDYVIQHQPQYKPHIRMQTPQAQNMQIQQPQAPQQIYIQPQALPQIRHVHQHQPQQQQSQQQQSQQHAQHGQHIHHHQHSLPKEVVLIEKICRGSMKTQATQTDTSKKPVLLSPRSNYKSSLVSTGAQTNGISNGRALIKKEPQKSPKMVPDGNQYFQLPTAFVPPTTRILPYPVSTQTDSITSLRSDQYSSTMVTTSEDIENNENLQQIDYVSNSLPRRHCNHSRMNDIDLYASLPRRDHSQQNANCRHITECDELFNVSSNDLYANVKRRASMGLPHQDSNELHSRMLRRQSMPVPSHFLKSSSSEEDEVPEEEKEIFIDFKPYISPRPSPKFRKKKLQKTCSDGEMLIDRYRGKDEEIAISSDGETGNIRVNECVLENKTEVPRQTYLSLPSNSDSEGIHLQKQKILMTSKRSMSLDENESYVDNFQRSSIDTEALDIEEQSETIVQKISKPAATQTTTPLPDLTYVSVTPKIAVTATSSTEAESQSQESRSRRRVSINRPVDIVELPPVEHIPKSQARSESGPKTNTDISECSTNTDEFVTCPDASKRTISSGIRGSPTTASSSSTQVPAQSSQTERTQEGSSFESASSLYSTRGDGLSEDIPPTSEEQMHEQFLLPKQEDNLTSSSTTAVQISNMKSPSHSIGSTSSGSLNVKNTELQISTKTDQQPMVGLDEVIEENGFESVQSATIQRRIRLEEERRRRRSNMNLDIEKENMRALASPIKKPTPPKLSPQRPPDLTDVTSPSKIKRVRPKIRRQLRKSSKDGGLIRSPDRPFHYKALSPQDDEQLLKAKSIESFRSVSPGSDSVFYSEVDGGIVDQQVHCLHCGKEVDIITAPSDSQEAIPQLENDEDEHIVKPPADFADSPITTKTAKSEERGGKDRESSDGSFSKLRASDSSPCVLPVDVEEEENDHIIYPGHYCENRYARLTDEDSESGEFGFRIHGSKPVVVAAIEPDTPAESSGLEVGDILISVNGTSVLDKHHTEVVKIAHAGCDTLKLEVARTLGVLMKEHQDQPAPAMYSGYLWRQSGQVKGSPNSKKWVRRWFALRPDHCLYYYKTNNDTQPVGAMILINHSLEKLSPDCGKPHAFKLGSPDGIPMIVAADSEDEANRWITLIEHISKQSSPWLGVSSRNLRMAPNAIQKPDCFGYLMKLGSRWNGWSKRYCLLKDACLYFYQDANSKNAFGMACLQGYKVIAMSSNSAGRKNAFEVVPPEQNLRHYYFASESEMDKKRWISALEYSIDRWIKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF09</accession>
    <name>T1GF09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF09"/>
    <dbReference type="STRING" id="36166.T1GF09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001937-RA">
        <property type="protein sequence ID" value="MESCA001937-PA"/>
        <property type="gene ID" value="MESCA001937"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003152_0_0_1"/>
    <dbReference type="OMA" id="CCITEEK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588241" description="TIR domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="7">
        <location>
            <begin position="116"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001937-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="32882" checksum="62AD4FCF0F9BB767" modified="2013-10-16" version="1">SFLSIIYLFFVSESLSSPTTTDVHSVHELRQIFWCVQSPGHHTNDAQFQMASELSWSLTQMAPNLKCSVRQETLQVPSPLQFLLFTASFKLEIQIWLYNHNLAFCCITEEKIDKNRIYDAFISYCHTDEEYVVKILEKLETGPDPYKFCLHFRDWMPGEFISKQIVESIEKSKRTMFILTKDFMDSVWSKLEFRTAHVASLREIRPRVILILCQEFEELGVLDPEIRSYIRTNTYLKWDDKLFWERLKYALPKKNIIEEKSEDIELEGCNFQNGLDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF33</accession>
    <name>T1GF33_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02088438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02088439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF33"/>
    <dbReference type="STRING" id="36166.T1GF33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001962-RA">
        <property type="protein sequence ID" value="MESCA001962-PA"/>
        <property type="gene ID" value="MESCA001962"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656522_0_0_1"/>
    <dbReference type="OMA" id="AEINCTT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF30">
        <property type="entry name" value="SIDESTEP VIII, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001962-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="18225" checksum="081BBA778A18580F" modified="2013-10-16" version="1">MSRELSVKCRERMKELFLLRPLIVKLQGENRPLSADTSYELSCSVFGSRPPATITWWKGSTPMTNTREAHPDANMTTSILTFTPTIDDKGKFLSCRAEQTLIPESGMEDGWKLDIYHIPVVTLELGSNVNSSFREGVDVFFECNIKSNPWIRSVSWRHNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFC6</accession>
    <name>T1GFC6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">YEATS domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 diacetylated at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac) or histone H3 diacetylated at 'Lys-14' and 'Lys-27' (H3K14ac and H3K27ac). Also able to recognize and bind crotonylated histone H3. May also recognize and bind histone H3 succinylated at 'Lys-122' (H3K122succ); additional evidences are however required to confirm this result in vivo. Plays a key role in histone variant H2AZ1/H2A.Z deposition into specific chromatin regions: recognizes and binds H3K14ac and H3K27ac on the promoters of actively transcribed genes and recruits NuA4-related complex to deposit H2AZ1/H2A.Z. H2AZ1/H2A.Z deposition is required for maintenance of embryonic stem cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of numerous complexes with chromatin remodeling and histone acetyltransferase activity. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with MLLT10/AF10. Also interacts with the SWI/SNF component SMARCB1/BAF47, TACC1 and TACC2, and the nuclear matrix protein NUMA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFC6"/>
    <dbReference type="STRING" id="36166.T1GFC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002063-RA">
        <property type="protein sequence ID" value="MESCA002063-PA"/>
        <property type="gene ID" value="MESCA002063"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051385_3_0_1"/>
    <dbReference type="OMA" id="VKPYHNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16909">
        <property type="entry name" value="YEATS_GAS41_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1970:FF:000002">
        <property type="entry name" value="YEATS domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="YEATS" evidence="14">
        <location>
            <begin position="16"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002063-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="26519" checksum="5DC15E4A0ED94D40" modified="2013-10-16" version="1">MSLNIPTDFGPDSGGRVKGVTIVKAIVYGNVARSFGKKREEDGHTHQWSVYVKPYLNEDMSAYVKKVHFKLHESYANPNRIVTKPPFEITETGWGEFEVVIKIYFHDPTERPVTCYHILKLFQSPIVEGEVNLNNDTKKLVSEQYEEIVFQEPTQYMQHCLTNIQPITSGQYKHDTDFELKKVKTLENIIDVKSKVKSEITALKEKLKLARETMTKFKTELAKVQKQPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFD3</accession>
    <name>T1GFD3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02046959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFD3"/>
    <dbReference type="STRING" id="36166.T1GFD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002072-RA">
        <property type="protein sequence ID" value="MESCA002072-PA"/>
        <property type="gene ID" value="MESCA002072"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_489440_0_0_1"/>
    <dbReference type="OMA" id="IENIFCR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097108">
        <property type="term" value="F:hedgehog family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008158">
        <property type="term" value="F:hedgehog receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005119">
        <property type="term" value="F:smoothened binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46022">
        <property type="entry name" value="PROTEIN PATCHED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46022:SF1">
        <property type="entry name" value="PROTEIN PATCHED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="467"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002072-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="63213" checksum="C632C2CBC8A1FA37" modified="2013-10-16" version="1">MKGSVKFCSSLIYICWTIFGLYSFYSKLQDGLDLVDLVPKDTNEYKFLQAQTSLFGFYNMYAVTQGNFEYPQSQQKLYDYHNKFVRVPHVVKNDNGGLPDFWLSIFRDWLKNLQEVFDKEYEENRITRETWHQNASSDAILAYKLMVQTGDIDNSVQKSLIPENRLVKDGIINPRGFYNYLSAWATNDPFGYGASQGKLRPEPIPYLHVPTEYTLKVPKSLPLVYTQLPFNLHGLKDTVEIKELIKNIRKICDDFENNQELSNYPSGIPFIFWEQYMDLRTSLAITLAFTISAAFICVFAILLNFRATVITIVNVIFSLMQLPGAMVLLGLKLSAIPAVIIIFSVGLILCFTVNIALSFMTSLGNKERRTILALEITMDSLIHGLCTFGIGVLMLLTSPFEFVIRHFFWLLFAVLCIGTFNGLVVFPIILSVFGPEAELISLEDPNRISTPSPVSSRSKRNANKTVITNDMGHSSSSSKQHSRKSHKYNINEKEPSLTTITEEPPSWKSSSSSIQMMNTEKSYSLNNESNLKAEPVHHHKNKLSDPLIFESASLKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFH7</accession>
    <name>T1GFH7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFH7"/>
    <dbReference type="STRING" id="36166.T1GFH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002119-RA">
        <property type="protein sequence ID" value="MESCA002119-PA"/>
        <property type="gene ID" value="MESCA002119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085588_0_0_1"/>
    <dbReference type="OMA" id="PHSTVHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="9">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="152"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26495" checksum="86987C49A273DE2A" modified="2013-10-16" version="1">MDETFLDILALPSPHNLIRERSLYCAFGGNTKIITRILEGDNDVCKKNIRIGIPTFRKLVHILPEKHHGWNKCIDVICYLYWLAETCSYRTVSNVLEISRHTVKTIIHRVLDEILALREIISHGNEVDYEGLGNMFCRRAKTNVFKKCIGAIDGTHIRIRCPKNRHDEYYNYKHFYSIQAQVVSSSQLLFTDVFVGYPGSVHDSRVFRNSPLYRRGNYPPEGYFILGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFH8</accession>
    <name>T1GFH8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine 5'-phosphoramidate + H2O = NH4(+) + AMP</text>
            <dbReference type="Rhea" id="RHEA:67916"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HINT family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFH8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002120-RA">
        <property type="protein sequence ID" value="MESCA002120-PA"/>
        <property type="gene ID" value="MESCA002120"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1397803_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF5">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002120-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22060" checksum="62FE6FADD083A72C" modified="2013-10-16" version="1">MKSCIFCDIAEKKSPDTVIEFENEELVIFKDIRPAAEHHFLAVPKVHFDINNLEASLKTLLENRGVDVNDALYGFHWPPFISVKHLHMHAMAPASGMAFKSRMIFRPNSLWFATDVGDHNKELLVDNLEKALKDLILSKSLNIDDAVFGFHVPPLISVWHLHLHALAPVSSIIPRARYKYTPTGKSKAESRAIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFV3</accession>
    <name>T1GFV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gastrulation defective protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFV3"/>
    <dbReference type="STRING" id="36166.T1GFV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002253-RA">
        <property type="protein sequence ID" value="MESCA002253-PA"/>
        <property type="gene ID" value="MESCA002253"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1126957_0_0_1"/>
    <dbReference type="OMA" id="FICILTM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051858">
        <property type="entry name" value="WD_repeat_GAD-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017">
        <property type="entry name" value="GASTRULATION DEFECTIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002253-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23127" checksum="8955FBEFB118A1F3" modified="2013-10-16" version="1">MLLTGESLQKGQTAANLRVFDVNTFELIKEIPVTNSHVVKTLWHPKLNQIFVGCGNGLVRCYYDEKRSFRGAILCASKSYRKKKPADVVGVSQIITPHALPMFRQEKSKSLRKKQEKDRLDPRPDLPITSGQGGRVASSGGTLSSYVIRNLGLSKRVDDDQDPREAILKYAKEAAENPYWIAPAYKKTQPKPIFNDEPDAKKAKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG64</accession>
    <name>T1GG64_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002374-RA">
        <property type="protein sequence ID" value="MESCA002374-PA"/>
        <property type="gene ID" value="MESCA002374"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2892481_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002374-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7087" checksum="E5F05F25336E6615" modified="2013-10-16" version="1">ILYLGTGKFVRSDYRNGFFGGLDGNDDSDAPMGLKIEAIIPILRLPSHSNRRWGMHSRCCTEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG91</accession>
    <name>T1GG91_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Growth hormone-inducible transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02039575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG91"/>
    <dbReference type="STRING" id="36166.T1GG91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002403-RA">
        <property type="protein sequence ID" value="MESCA002403-PA"/>
        <property type="gene ID" value="MESCA002403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050797_2_0_1"/>
    <dbReference type="OMA" id="WATHCAI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF112">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="19501" checksum="F49144E3BCAD7A1B" modified="2013-10-16" version="1">MSMLGTIALMMGSGMLCQSIPYSEGIGVKQLAWATHCAIIGAVVAPLCFLGGPLLTRAALYTGGIVGGLSTVAVCAPSDKFLYMGGPLAIGLGAVFASSLASMWLPPTTALGAGLASLSLYGGLVLFSGFLLYDTQRIVRSAETHPTMYMDRPYDPINKSLSIYMDTLNIFIRIATILAGGGGNRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGU2</accession>
    <name>T1GGU2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGU2"/>
    <dbReference type="STRING" id="36166.T1GGU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002623-RA">
        <property type="protein sequence ID" value="MESCA002623-PA"/>
        <property type="gene ID" value="MESCA002623"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060885_0_0_1"/>
    <dbReference type="OMA" id="INSEHIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002623-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="296" mass="32884" checksum="055F2204A6415094" modified="2013-10-16" version="1">MNSISMCEENATLEEGIGKTKNFLMNLGFYGNTPFLFPMYGCGEIPQCFCRLCAVFGGIYCLKKPIENIEEIDSQISFTAENQVIKTKNIVFGSSKIPESLLSKSSNPEKRCGALSRGIFITDKPLGSEELNAGGGGVNFLKLPGKNGGAFILQLSHYSGTCPKGLYLIHVTTKLENSPEEDLKVFKEKLFNENVKPIYSAYFNLPPCFLCQGNVINSEHIFECCGSFFELDYDKSMDNAESVFKKLYPDVDFLPRAPDPEEIIIEDEDPTAISENSLPEDLKAQLLLDENKENSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGW8</accession>
    <name>T1GGW8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BET1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Golgi vesicular membrane-trafficking protein p18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE involved in the docking process of ER-derived vesicles with the cis-Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with SNARE complex members GOSR2, SEC22B and STX5. Interacts with LMAN1/ERGIC53. Interacts with STX17.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the BET1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02178081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGW8"/>
    <dbReference type="STRING" id="36166.T1GGW8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002649-RA">
        <property type="protein sequence ID" value="MESCA002649-PA"/>
        <property type="gene ID" value="MESCA002649"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086133_2_1_1"/>
    <dbReference type="OMA" id="GHRNYMC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000026">
        <property type="entry name" value="BET1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="20">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002649-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13647" checksum="CF7CC8BFD4D293AE" modified="2013-10-16" version="1">MLVLVASSSMRRSNYSYQPLSQNVPGPSSMQDDLEQENERQAEELKHKIGALKSLTIDIGNEVRYQDKLIRGIDDDMDRTGGFLGNTMAKVLRLRKNGGNKHMCYMFLFILFIKECSSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH06</accession>
    <name>T1GH06_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vertebrate heat shock transcription factor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH06"/>
    <dbReference type="STRING" id="36166.T1GH06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002688-RA">
        <property type="protein sequence ID" value="MESCA002688-PA"/>
        <property type="gene ID" value="MESCA002688"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_583029_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010542">
        <property type="entry name" value="Vert_HSTF_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06546">
        <property type="entry name" value="Vert_HS_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vertebrate heat shock transcription factor C-terminal" evidence="5">
        <location>
            <begin position="328"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="104"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002688-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="469" mass="51477" checksum="07FD596BB46B3F7E" modified="2013-10-16" version="1">MRGTQENFDSRLSVMKQENEALWREIASLRQKHMKQQQIVNKLIQFLVTLVQPSSRNMTGVKRQFQLMINDAPHSSGNNRRNSEGDNNGPVIHELTEELLEATDDPDLLTSTPNVNSPGGGNYRVDSVEERPASSASYNSNYDDVNAMDDEAIQLIEQQPFEQQNYQIQNNNSNTNPSPIPGAQYTSQEPQYFETENNVVQTRRSRRRKAATTEAGTSKVAKFANNQNGSPVTVSGTAPAPLLIKTENDIDQLLMMQSPNNSSNSPASVTPLTGDEYINGSFLSNEVPKDLFDDQSQQPSPVVGGFDLAGGSYNDKIDLNAGKAPPSSSVVSSASPVLGQDSSDSSLQLATVAKSKMTKPNELLRLNSMEDVNNHLDGVQENLDSLKDILMGEGYSLDPNTLLGILNPYMSLSSDMEPGAPPKELTYEPFNGDLTDMLNTDLNYQNKTSDDQNKSPQAVQSILNTPFHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH23</accession>
    <name>T1GH23_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002706-RA">
        <property type="protein sequence ID" value="MESCA002706-PA"/>
        <property type="gene ID" value="MESCA002706"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_573162_0_0_1"/>
    <dbReference type="OMA" id="CSQPPRH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002706-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53700" checksum="CBB7F3C29D45E501" modified="2013-10-16" version="1">MFEEFSVRLQDPEWQVRQHALRVLYDVLAVLNAKSDMYMPPIIPKLVENLGHPAPTVRKGALDSLRMYLAETAHPENVILQILDIGLNQRISNEPYGGRMTCGVLLSLPALIQSTLHTKKRNYILRTTIDMLVNKMTHVTHQEITLKVLTKLKELIGPREFSEYMSHAAFREYELLCSVYGVGEKRHMHQSEPKVQLNSSRSSWKLMSSSFDSDDPKESDGYWRSSDEERTLNYTFTKLPSITPPLVEKSAPSTMQPEKIIMETEIKINNDTVTMRILEEGDKYESSTDDDYISRSVVEPTGLVQVFTDSEVDDGLTPKRVTFGGEVVKMRTPDSDASHEEVVKSEVKQKEKSFDLLTIEITNDNTKSPPIMSPSQGTQTSPPPSPSKQSPRSPFKRLSIASVDSIVSPKTQHKEIEVLHNLQRDPSPNRSTPKRSAETTPTKEPSPPPTKGPSPPPKNWEELGIVSENVLKNLKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH86</accession>
    <name>T1GH86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WD repeat-containing protein 48 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Regulatory component of the Usp12-46 deubiquitylase complex. activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. The complex deubiquitylates the wg/wingless-signaling receptor arr/arrow, which stabilizes the receptor and increases its concentration at the cell surface; this enhances the sensitivity of cells to wg/wingless-signal stimulation. This increases the amplitude and spatial range of the signaling response to the wg/wingless morphogen gradient, facilitating the precise concentration-dependent regulation of its target genes. Together with Wdr20 and Usp12-46 required for wg/wingless-mediated signaling in the wing imaginal disc and for wg/wingless-dependent regulation of intestinal stem cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Catalytic component of the Usp12-46 deubiquitylase complex consisting of Usp12-46, Wdr20 and Uaf1; regulatory subunit that, together wtih Wdr20, stabilizes Usp12-46. The Usp12-46 deubiquitylase complex associates with arr/arrow; the interaction leads to deubiquitination and stabilization of arr/arrow.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02044098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GH86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002776-RA">
        <property type="protein sequence ID" value="MESCA002776-PA"/>
        <property type="gene ID" value="MESCA002776"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014960_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000543">
        <property type="entry name" value="WD repeat-containing protein 48 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021772">
        <property type="entry name" value="WDR48/Bun107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11816">
        <property type="entry name" value="DUF3337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002776-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="596" mass="66566" checksum="E0A6DE8C9EF9C1FC" modified="2013-10-16" version="1">MLSQKNCHRKKMNVSFVIRDAEEKQHRNGVNSLQIDQNTGKLYSAGRDAIIRVWNTKNIDSNDKYIQSMEHHNDWVNDIVLCCNGRNLISASCDTTVKVWNAQKGFCMSTLRTHRDYVQSLAYAKEKEQVASAGLDKAIFLWDVNTLTALTASNNTVTTSSLAGSKDSIYSLAMNPSGKIIVSGSTENILRIWDPRSCVRKMKLKGHAENVRALVVSNDGTQVVSGSSDGTIKLWTWDNKGSRDKKIFVTELRNPNNSTLVCEEKAPVLSMCYNLDRSGIWVTTWNSDIRCWKLPNYDKSTLSNSLENGQYSLNGVVSGSGVELACIKGGAAIKKCAVLNDKRHILTKDSEDNVAIYDVLRIVKKEDLGKVDFDAELNKLNQKIYIPNWFTVDLKTGMPTIVLGQDEVNCFAAWVSMEAGLPEFADQSSDAKINYGSLLLQALLEHWTPPQSLQSGGLDDEAKGNNYFQVPKHTPLIFSEVXXXXXXXXXXXXQVPKHTPLIFSEVGGRTVCRLLVRDAAGESESSLLQETIPQWVTDIVIEKNIPKFNKISFYLQPHPQMVKPDRMKKVNSGSQHGLKNSMSLHMEAIWRSDVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIG3</accession>
    <name>T1GIG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="5">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02087886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIG3"/>
    <dbReference type="STRING" id="36166.T1GIG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003241-RA">
        <property type="protein sequence ID" value="MESCA003241-PA"/>
        <property type="gene ID" value="MESCA003241"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124749_0_0_1"/>
    <dbReference type="OMA" id="NDITWIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003241-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20891" checksum="82D2E1A22A505425" modified="2013-10-16" version="1">MYAQDSIDLLTNSGIQFRKHEEDGIDPIDFAELLMSSGIVLMDNIKWLCFHSGYDFGYLLKLLTCQNLPAEETDFFELLRIYFPTIYDIKFLMKSCKTLKGGLQEVADQLELLRVGPQHQAGSDALLTGMAFFKMREMFFEDNIDNAKYCGHLYGLGTSFLNNNNNNNNNFHENNGENNNATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIL0</accession>
    <name>T1GIL0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02114793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02114794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02114795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GIL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003291-RA">
        <property type="protein sequence ID" value="MESCA003291-PA"/>
        <property type="gene ID" value="MESCA003291"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_14_0_1"/>
    <dbReference type="OMA" id="NGHGEMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000011">
        <property type="entry name" value="Myosin 10A, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059004">
        <property type="entry name" value="MYO15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF26">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26570">
        <property type="entry name" value="MYO15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="1"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="11">
        <location>
            <begin position="739"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="11">
        <location>
            <begin position="1891"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="963"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1011"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1054"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1392"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1442"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1492"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1558"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1585"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="979"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1011"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1076"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1097"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1392"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1453"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1498"/>
            <end position="1527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003291-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2324" mass="264925" checksum="8072F4897C05B692" modified="2013-10-16" version="1">MQYLAAVVPGGGNASALINEQILEAAPLLESFGNARTVRNDNSSRFGKYLEVFFKNGSIVGAKITQYLLEKSRIVTQSNGEQNYHVFYEMLNGISETERSKYGLLEADKYFYLNQGGLKYPSGRVDWQGLLGSMQVLGISESERESIIKVLSSVLHLGNIYFHRRQLRHGQEGVEVGSDAEIKWAAHLLQISAEELHSTLTSRITETKTERLHTPLGIDQALDSRDAFAKALYSGLFNWLVSRINMILHKGGSHNAYKICILDIFGFENLEENSFEQLCINYANENLQLYFNKHVFKLEQADYHLLAKKPVGIFHLLDDESNFPCGTDLSFLEKSHYNHALNDLYSRPRIGAQDFGINHYAGTVWYCVDGFLDKNRDYLRADVLDLLESSSLTLVEEITKHLKAQRDYGKTLPKGHNGRFVTMKPRTPTVAARFSDSLQQLLQSMGRCNPWFVRCIKPNNDKQALKMDMPCVMQQLRYLGMLDTIKIRQRGYPVRLRFQHFVDRYRYVLKGTYLHGTPYRDLCRLILDTMLESNMNTNDYQLGATRIFLREGLHRQLEIGRADSLKVAANVIQRNVRGMLVRRKLDRKNKAALKIQSVWRGHRQPLHRGRVVRKKYLKMKRDLRYRKESSRSEQSHHIPIQIQNHHQTHVQKKSITQAQPPIHKKSDVNELAFLDIPAELSYLFKKVEDWTPVHGDRHLTKVAGVVSNSPQSFEVPNDVDQFAFGKFSSVYCNDIKLCPRSEPISSPFMSKAASKDQDFVDSIAIFKLILRWSKDESLRGNSEKMLVDYIISKGQNSYALRDEILVQICNQLYGNPNEMQTQKLWFLMDQCLSSFKSSNVFKKYLLKFILDNSPKNLTESLVKKVLRKSFLSHRNFPTSWIEWRAFHKFSDIALCLVLPDETAQTVAIDSWTTYDGKVLVDSSGLDFVLDMISEVELFPSFPVTRNDLFKYGSKFDRFILPNGEVKRPQVPPPDPPKISITSDKSLGRSSQGRKLSNDILSRNSALNERYFEKEKSRSRSMDNLLLDDNETEPEKDLTAFANIGLSKSKLNERYHSTERLNPTNNELTPRYTKYQSGSRRSYASSHSKHMEKPEYLSRSSAMSDTSEAPSLASHVKRVRVPSQASDVDQFLDDLFRPVLDGSLDELSDARSLAASIRGRGKDDNFWDSIPISSDLNSLQNGSNISRQIKGGYSPLDSVDVLPELDSLSNKNKFNGSYQDQIQRAFLQSAMAQNLQIQQQLLAQNQALQTLLQQQQKNPGAVQISTTKVTVQKAIIPRQTRKISAENGEVLIPPPPPPPMPPPIEFKDPSEARPFLDPYGRAKTVRIGKWRWPPPNSNQIHETEEDFLMFKMRQNQRKTTPQARFNGDDDDWQDFEIEKKKSVISKETITISTADSTDSVAPSSAPVKSKKKSFEVGAQRPPPGSVGKLKLSSEMRQRLEQLTAGHSVRSNVSKKSDHQSPSKLENTKRMLLEQQLGMVGVVPPHMRSFEDIDKWQGLPPAPSVAAPPPPIHPPSMAPPKPPPVPVSPGEPIKEYKQPSVPNEWESVEIAKEMSEMSIMSMDQNRARSRSRSRDRDDFSESIWDRSEVEGHSLSDKEREKEKEKSIEDKQEKEIVSKRSSFMPVTYKTTARIETDSHSQITPSPTFKTHMAEKYEHERKIKTINRKENGTSDWERPISIPAPVPLPSSAKTSNTSLTYNRVNWKLRVRKEFFFPKEQVKSPAAIDLIFLQIVADVFETAPCLRLSMQEKNSAKNMLSSHGISVDNVRQQKIPISVKKYLIDMARSWALYFSRIFCVQGPPQYPDICVMAVSENGIYLARRDNDSFLVLHSFNFNNVDGAITLPRPTALQLNLSNGNRITRKYTLTQFASKYFRNENENEEMSNKKLMELVRWQGSPIRAPLLRLPVELSDLALECFECILRYCGDIPLDIHLTEVKCVYTVLMHCHKYLALRDEVYCQLMKQTTANNSPNPDSIQRAWRLFSILAAYFGCSDALHPYLVDHLSSIASDRRKPCYGTAAVCLANLRKTARCGGRKNVPSVDEVTAVSVGRSARRQIYRLPGGSERVVNTRCSTVVADVIAELCNLLGIHSEEEQQEFSLYCIVEGDAFTLPLSFDEYILDVTTELLKSGQPFYLIFFMPDYLEGLLFEMPANGVLSQESVRDISRLAAILHRAADLNHVPTMKEIKFLLPKPILAVRDIRPMQWVQFVQTAWPTVADLSPGLVKAQFLNVLSKWPLFGSSFFAVKRIWKKDSSTVIENSNSSWKEMILVLNRQGVMFLDPSRHETLQHWHFMEVISTRKTDGKQPSLKWKTVELRHVVNSSLCGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ67</accession>
    <name>T1GJ67_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ67"/>
    <dbReference type="STRING" id="36166.T1GJ67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003511-RA">
        <property type="protein sequence ID" value="MESCA003511-PA"/>
        <property type="gene ID" value="MESCA003511"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009711_1_0_1"/>
    <dbReference type="OMA" id="FLRTHGN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052248">
        <property type="entry name" value="Arf-GAP_FG-repeat_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134:SF3">
        <property type="entry name" value="ARFGAP WITH FG REPEATS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134">
        <property type="entry name" value="DRONGO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="6">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="74"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003511-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="479" mass="51303" checksum="C2A328B12F2B5861" modified="2013-10-16" version="1">MEIKRGLTPPHRVKSISMATFTPEEIEFLKSHGNEECAKTWLGLWDPKRNVHQDQRELIIDKYERKRYYLEPGSPLKSITNLSSSNNKTNSNTNNSNSNSSYNHLSNGLKSITLTPPSSRGSHSSLNGSLNGSTGGVNGFNNFLNRKTSNSSVDCGSSINGNCNGSSGFSSDSDFADFGSANIFDATSTNNTINNSVSSNNSSFNSSFKQQNGSILDLHNGRSSAPAVNNENFADFDHAPIYNAAGGNQIRNPGSNSIPCHHHQQQQTFTMFQQNSNGNPINQQQIQWNSLWPASPASSSNNNNNWSLPVSTTNPQMNGNSNNTSMKNSTSTVTPDRYAALKDLDEEFRETKAQAAAAQAVQQQGPTVPTNGNNVMGSPNPFKSQQIANPFQATTNIQMFPNTAPIVPQPLFGSGFNANGFTNGNNFGGSPAYVPNGSCGFGFGALQPQQPIAATGMGAFSNPFAATVNSSGNSNNPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJN2</accession>
    <name>T1GJN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRO8NT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJN2"/>
    <dbReference type="STRING" id="36166.T1GJN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003682-RA">
        <property type="protein sequence ID" value="MESCA003682-PA"/>
        <property type="gene ID" value="MESCA003682"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_695011_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012591">
        <property type="entry name" value="PRO8NT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012592">
        <property type="entry name" value="PROCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08082">
        <property type="entry name" value="PRO8NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08083">
        <property type="entry name" value="PROCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRO8NT" evidence="1">
        <location>
            <begin position="94"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="PROCN" evidence="2">
        <location>
            <begin position="246"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003682-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="46675" checksum="35D81CE13D78330D" modified="2013-10-16" version="1">MSIPPYNINQWAAQIMAQQYAAAQQQIHAHQMANQIQQIPPPGAPLPPPASGNLLQKNELISEEKLFEKSQKWSQLQGKRFAEKRKFGFIDAQKEEMPPEHIRKIIRDHGDMTSRKYRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVQVLYHITGAISFVNEIPWVIEPLYIAQWGYHTPNVVYIKTEDPDLPAFYFDPLINPISHRHAIKVQEPVIDEDEDFVLAEELEPFLQDTPLYTDNTANGISLLWAPRPFNLRSGRCRRAIDIPLVKGWYKEHCPPGHPVKVRVSYQKLLKYYVLNALKHRKPKPQKKRYLFRSFKLTKFFQTTTLDWVEAGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNVIFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJX5</accession>
    <name>T1GJX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenylate kinase active site lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02026077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJX5"/>
    <dbReference type="STRING" id="36166.T1GJX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003785-RA">
        <property type="protein sequence ID" value="MESCA003785-PA"/>
        <property type="gene ID" value="MESCA003785"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_0_1"/>
    <dbReference type="OMA" id="VYHEQTA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="5">
        <location>
            <begin position="137"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003785-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="232" mass="26334" checksum="B25870E8371B424F" modified="2013-10-16" version="1">MAPTDFKTLPKQRYEQPIGINAILLGPPGSGKGTQAPLLKEKFCVCHLSTGDMLRAEISSGSKLGTEVKKIMDEGKLVSDDLVVNMIDKWIFIGWIPKNCCTSREIDELLEKRKTPLDAVIEFSIDDNLLVRRITGRLIHQASGRSYHEEFHPPKVAFKDDITGEPLIRRSDDNAEALKKRLESYHRQTRPLAQYYALRGLHFAVDAAQKANNVFEKIDSIFMKQKEERVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJZ0</accession>
    <name>T1GJZ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02029556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJZ0"/>
    <dbReference type="STRING" id="36166.T1GJZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003800-RA">
        <property type="protein sequence ID" value="MESCA003800-PA"/>
        <property type="gene ID" value="MESCA003800"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1612715_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003800-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18642" checksum="9D0F3811FEF5394C" modified="2013-10-16" version="1">MRDANYGEVVKRIKGKKNGFGPGKQDWGHVTVRKGAHMFYWLYYTTATPLHTRRPLAIWLQGGPGASSTGYGNFEELGPLTLELENRNWTWVKDMNVMFIDNPVGSGYSYVDAATNSWGATQRVLLTESKGVDFYDILKPVKGPNKARLFYNDFGENDEKNLGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKQ8</accession>
    <name>T1GKQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKQ8"/>
    <dbReference type="STRING" id="36166.T1GKQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004085-RA">
        <property type="protein sequence ID" value="MESCA004085-PA"/>
        <property type="gene ID" value="MESCA004085"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2747439_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAD" evidence="2">
        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004085-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="9150" checksum="19C8A5814B57B511" modified="2013-10-16" version="1">MNQKKQQDAVINELKTKANAVREKKLLQQDEVYNGALEDILLGLKNEPYRRADAVRRSQRRRIDSNRFSRTMDELEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKR1</accession>
    <name>T1GKR1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099593">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004088-RA">
        <property type="protein sequence ID" value="MESCA004088-PA"/>
        <property type="gene ID" value="MESCA004088"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909068_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004088-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15630" checksum="1689224CC8C3B7C8" modified="2013-10-16" version="1">MSCDLYKPCIFVCYENPRVKDWFMMSSPIPTLLLCLTYAYIKSVALHTEFQRSKLASIKLIFQTMRKQHRRGCSLQKTLFEWHSIYLIINYKRRLYSLNVESAPLKSATDHGKNGKRIKLIVPYGKDSIYKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKX1</accession>
    <name>T1GKX1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKX1"/>
    <dbReference type="STRING" id="36166.T1GKX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004152-RA">
        <property type="protein sequence ID" value="MESCA004152-PA"/>
        <property type="gene ID" value="MESCA004152"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111638_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004152-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="13349" checksum="F0BA8A78C4E50999" modified="2013-10-16" version="1">MPQSLVLQKQRILPTSKLSFLLLVHISKEYLQLEPIGLVFVFFFALILVIQFTAMLFHRFGTISHILASTELNLCKKKSEDLSQDALIDKMNVNQALYNFAFERINKIHNLEQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLE1</accession>
    <name>T1GLE1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004341-RA">
        <property type="protein sequence ID" value="MESCA004341-PA"/>
        <property type="gene ID" value="MESCA004341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3415385_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="27" mass="3227" checksum="A698F161DFBF0DEB" modified="2013-10-16" version="1">MKTFAMSWDCPNQQKPYQNLDNGFKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLP8</accession>
    <name>T1GLP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Solute carrier family 25 member 40</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02068252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLP8"/>
    <dbReference type="STRING" id="36166.T1GLP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004461-RA">
        <property type="protein sequence ID" value="MESCA004461-PA"/>
        <property type="gene ID" value="MESCA004461"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1225923_0_0_1"/>
    <dbReference type="OMA" id="APRIRFH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="11"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004461-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23150" checksum="D79D3A87E0985DB2" modified="2013-10-16" version="1">MEDERFRIRPHQQICSAITGAVTTSLFMTPLDVVKTRIQTQQKALVSNKCFLYCNGLMDHLCPCPNGNGNGTGKLGGIPKAPTVPLNGTVDAIIKISRSEGVYSLWSGLGPTLVSALPSTVIYFVAYEQIKRQMTTIHHHFYEKNSDRMPIYVPLMSGVLSRIFAVTAVSPIELIRTKMQSQKLSYSDLSKAIRSLSGMRDSLLCGKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMC8</accession>
    <name>T1GMC8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMC8"/>
    <dbReference type="STRING" id="36166.T1GMC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004699-RA">
        <property type="protein sequence ID" value="MESCA004699-PA"/>
        <property type="gene ID" value="MESCA004699"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203573_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sema" evidence="3">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004699-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12260" checksum="350F6C2365129773" modified="2013-10-16" version="1">MLCYVMDEMKLIENFTLQANLTDLPRNQYVPGIGLGIGKCPYDPLDNSTAIYVEKGNPDDLPALYSGTNAEFTKADSVIFRPDLYNSSTGRMAHRFKRTLKYDSKWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN09</accession>
    <name>T1GN09_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004948-RA">
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        <property type="gene ID" value="MESCA004948"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004948-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="241" mass="26984" checksum="474B038A31688A2D" modified="2013-10-16" version="1">MWDIDDMSWRNYMLWPIPLETEASGQQNILKSCRDFHVRNWGNKGPHYRIQALQTFFGPMQHVYHFNGNLRPNLNPILVFGCGQLSWAHVGFGCAPLGPRQVIQTLCNIILKGISVAVLGTCWFLDMAVLAIYGVMDFATVTPVSQNITQRDIKVYRYGTLSLAALFALYERTVVDSFYSMQIMWITSAVPVLLTTSSSTSFKGLTWAVAASYKIVLCAILYKTNTQVTLASDKVPFVGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN33</accession>
    <name>T1GN33_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004972-RA">
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        <property type="gene ID" value="MESCA004972"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004972-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9853" checksum="D33F4FD5272C008E" modified="2013-10-16" version="1">MLLIKFGRSWKIFKKLNLKSIYTFCRKNTRGGFGPSYIENSEDSKANDAFKKIYESMIITKILITLPSNYSHFYCSTPKEERTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNQ1</accession>
    <name>T1GNQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019779">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005209-RA">
        <property type="protein sequence ID" value="MESCA005209-PA"/>
        <property type="gene ID" value="MESCA005209"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194194_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588427" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="19"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005209-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7226" checksum="307734126960B7BC" modified="2013-10-16" version="1">MKYLILNILIIAIAAISNAQSCVGKGNGYHVNPNDCSKYYYCINGRITNFDCRTSNLGWARDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP24</accession>
    <name>T1GP24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c oxidase assembly protein COX15 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP24"/>
    <dbReference type="STRING" id="36166.T1GP24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005340-RA">
        <property type="protein sequence ID" value="MESCA005340-PA"/>
        <property type="gene ID" value="MESCA005340"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017627_0_1_1"/>
    <dbReference type="OMA" id="AFVCYSW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005340-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41191" checksum="808720045FD09FA5" modified="2013-10-16" version="1">MVFVAVVLGGVSYYFLYYSTTEVVSKALEEATRRGVTRLTESGLSMVTWKLLGEQRPKTQEEWIAEFEKYKEFPEFKIKNQNMTVEEFKFIYFMEYMHRMWGRAIGAFFALPAVYFWTKGYFAKGMKPRVLIFGTLIGLQGLMGWYMVKSGLEDRFHGENDVPRVSQYRLAAHLSFAFVLYTLFLWSSFDHLIPAQKLKEVTLQAAKFRKFAHVTKGMVFLTALSGAFVAGLDAGLVYNSFPKMADKWVPTDIMAIEPKIKNFTENPTTVQFDHRILGITTLSLISGLWLLSRRRILPKRAYWAANAVGAMGWAQVGLGILTLLNYVPVSLAACHQSGSLVLLSMAVWLTHELKFVKYIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP89</accession>
    <name>T1GP89_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005410-RA">
        <property type="protein sequence ID" value="MESCA005410-PA"/>
        <property type="gene ID" value="MESCA005410"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576602_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005410-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9293" checksum="2A195699321549DA" modified="2013-10-16" version="1">MLLKGGKNEEKRSPTNQMGRSSGERRQRTGNTKLVSWILPKDRSIKSSKLHPVTFMNMEVGTDVLSLFLFIKLHFSNINFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPF8</accession>
    <name>T1GPF8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED8</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02033611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPF8"/>
    <dbReference type="STRING" id="36166.T1GPF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005487-RA">
        <property type="protein sequence ID" value="MESCA005487-PA"/>
        <property type="gene ID" value="MESCA005487"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_580450_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025259">
        <property type="entry name" value="CCDC34/181"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074:SF9">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13904">
        <property type="entry name" value="CCDC34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Coiled-coil" evidence="11">
        <location>
            <begin position="293"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="240"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="377"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="471" mass="53909" checksum="692884379B9164F1" modified="2013-10-16" version="1">MQREEKQWEMTLEQILSRLNDLKLSIGNMIQVLETQYETINWPTFLDNFALISSHLTGISKILNRRDMGGCLLRNRTFLPLTLSQDRDEHLVQIMKDVTKPDPTAEARMIAHEQKANSLNADTANKQVMQFNKVVGHISDIISEHRDKFETESSARAGIQQTSSMTDTQALVAAVGIGKGLLKMPMAPMGGGPGQVMLPPGGIRPPSMMSSLVWTKMVKKPKGLLVLEEMAAAERQLQEPFLRHGEHSNSNTTGSSEMVPNLDLTSNESYPSTDNELWYGKACEMSKMITKPDAFKAWLSAKKKSEFYESMRKKALEEELEKTKAEKKRIADEKYKEWLKKKSATSLVKKKSSTQLTSPKQTRSQEEIDHNFEQWLLKKAEYDRHLKERIREELEQKRILEDTKKKISEKIYNKWLETAPYKPKPVPMNKGLESLRGSTTEIHKNPEPWRTVIGEEVDDLSTCEEVPEMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPN0</accession>
    <name>T1GPN0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylserine decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.65</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylethanolamine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02095963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPN0"/>
    <dbReference type="STRING" id="36166.T1GPN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005566-RA">
        <property type="protein sequence ID" value="MESCA005566-PA"/>
        <property type="gene ID" value="MESCA005566"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029061_3_0_1"/>
    <dbReference type="OMA" id="RWVANQC"/>
    <dbReference type="UniPathway" id="UPA00558"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF6">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005566-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="409" mass="47267" checksum="9E8D0B95F5845B0F" modified="2013-10-16" version="1">MVSYLVPRNRIFSKYTLRHIPIKSNNTSPNQNSGRWPKWSTFRRNISSNNHNQRVENIVQQQNNKIKSHKDDTNGKYQSSNHGSNSSRNKWFSWPGLLFRWAPMGVCILALEWQYHHYECDKDKLPRTASNFQSTVYCSLPLRLISRCWGWLADVYVPVNLRPYVFGFYSKTFNVNLEEAAVPDFKYYRSIADFFTRSLKDGARVIDEKCDLVSPSDGKVLHIGTVNKSLVEQVKGITYKIDSFLGPNTWQHRMILKKIAPGDYHRFHSAAHWEPNLRRHFPGQLLSVNPKIAKWFPGLFCVNERVVYVGKWQHGFFSYTAVGATNVGSVQIYIDEKLKTNQWMGMKSINKAQYDDLTISNKNFEKGELIGQFNMGSTIVLLFEASKNFKFKVEPGQKVSVGEPLGEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQU2</accession>
    <name>T1GQU2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQU2"/>
    <dbReference type="STRING" id="36166.T1GQU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006007-RA">
        <property type="protein sequence ID" value="MESCA006007-PA"/>
        <property type="gene ID" value="MESCA006007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1437844_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="20713" checksum="AB026DB57A9634AB" modified="2013-10-16" version="1">MGAQKKFSTKQMGNRRPGRPKQKWLKLVNLSLPSKLVSPAKVQDYQQSLTRNNVIRSPQTQTNIPGYVPITTTTNTFTDDINNSDYVCMTAHLKRNPPQPQMSHGPPDPVPPPSNRLYTKEPIPIAKPAVTNQPPKPINKVDLNSHQPPPISPTPSQNSTGSGSGRRLTKNLLPYSVTPPKSAGPTEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR79</accession>
    <name>T1GR79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02027319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR79"/>
    <dbReference type="STRING" id="36166.T1GR79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006155-RA">
        <property type="protein sequence ID" value="MESCA006155-PA"/>
        <property type="gene ID" value="MESCA006155"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326672_0_0_1"/>
    <dbReference type="OMA" id="EIWITFF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006155-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11578" checksum="2D3C2762E90B91D0" modified="2013-10-16" version="1">MMLFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGCFSMYTGIIYNDIFSKSLNIFGTHWKVDRNVTDVLANEYLQLDPATAEYEKDPYPFGMDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRA4</accession>
    <name>T1GRA4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRA4"/>
    <dbReference type="STRING" id="36166.T1GRA4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006182-RA">
        <property type="protein sequence ID" value="MESCA006182-PA"/>
        <property type="gene ID" value="MESCA006182"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877787_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000021">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="4">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006182-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15344" checksum="37A6EB4CCF93B19D" modified="2013-10-16" version="1">MSSASLAPINSVLCLENLQPTNFSRYLELCKSSRESLKKDPDFKRILPRRCALVAPTFCDFCGEMMFGLVRQGLKCDGCGQNYHKRCAVKIPNNCLKTNESSRRSSNQQPSKSPMGGQLHPCFQRISSKMTHLVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS89</accession>
    <name>T1GS89_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-19kD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase 19 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA8 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02130316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS89"/>
    <dbReference type="STRING" id="36166.T1GS89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006539-RA">
        <property type="protein sequence ID" value="MESCA006539-PA"/>
        <property type="gene ID" value="MESCA006539"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081931_2_0_1"/>
    <dbReference type="OMA" id="TIPENDY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016680">
        <property type="entry name" value="NDUFA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13344:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13344">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CHCH" evidence="14">
        <location>
            <begin position="11"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="66"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006539-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12260" checksum="0C13DCC75244E0CF" modified="2013-10-16" version="1">MNFFQKVKKSCYEEFMQYATCLDRSSGDMAFNKCRKTQQVFDSCVKDNLGIDRPDFGYFCEARIHKSDRPAPPKPEKKVFPDATPGLPEDAPRPAAKYGSRFHWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSN4</accession>
    <name>T1GSN4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006694-RA">
        <property type="protein sequence ID" value="MESCA006694-PA"/>
        <property type="gene ID" value="MESCA006694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662806_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577668" evidence="1">
        <location>
            <begin position="21"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18435" checksum="D1D057F3E6F3050F" modified="2013-10-16" version="1">MTFKIFVVLLGLSSIGVTFAYSTIGPEQVTLPSCIKCTGNVIYNIIYKLFIQGTTPKPSYETATKSPTKPPNPLNCCTEDDMKIIERYQKQFNYKAESSPRLSYIDQSPSSDLKQLLENLKRPFYAKPRPYRMKPRYNYYSYPKKPVYVYERNDLKVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT34</accession>
    <name>T1GT34_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02149195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT34"/>
    <dbReference type="STRING" id="36166.T1GT34"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006854-RA">
        <property type="protein sequence ID" value="MESCA006854-PA"/>
        <property type="gene ID" value="MESCA006854"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_3_2_1"/>
    <dbReference type="OMA" id="VQDENMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.870:FF:000001">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="86"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="407"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006854-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="554" mass="62155" checksum="1B1F996A0D3C01D0" modified="2013-10-16" version="1">MQSSKLDSNSRHKHGEQGDGVRGLKALGVRDLNYRMAFLACSVQATTSRFGGTDLPMSEVTSEDMKKQMTDAEWNKVYEMSKDRYIYDNLIKSLFPAIYGNDEVKRGIILQLFGGVGKTTHEKTTLRGDINVCIVGDPSTAKSQFLKQVSDFSPRAVYTSGKASSAAGLTAAVVRDEESFDFVIEAGALMLADNGICCIDEFDKMDPRDQVAIHEAMEQQTISIAKAGVRATLNARASILAAANPINGRYDRSKSLQQNIQLSAPIMSRFDLFFILVDECNEVVDYAIARKVVDLHSNNEENVDTAYTEKKYITFARQFKPLINEEAAKLLVENYGHLRQRDTGTSGKSTWRITVRQLESMIRLSEAMAKLECANEVQAKHVKEAFRLLNKSIIRVEQPDIHFDDEDVAHTAGDDDGNQDVEMQENGENGEEEDGLQKKRITLSFEDYKNISNMLVLHMRNEESKAEDLGTDTGMKKIEIINWYLEQVADQIETEEELISRKTLIEKVIDRLINHDSVVIPLKTVELKTKGGAEDSEDTDDDTLLVVHPNYVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTY8</accession>
    <name>T1GTY8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Choline O-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.6" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02144898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTY8"/>
    <dbReference type="STRING" id="36166.T1GTY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007187-RA">
        <property type="protein sequence ID" value="MESCA007187-PA"/>
        <property type="gene ID" value="MESCA007187"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2090802_0_0_1"/>
    <dbReference type="OMA" id="CTNESTR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004102">
        <property type="term" value="F:choline O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008292">
        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF14">
        <property type="entry name" value="CHOLINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007187-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13498" checksum="AF9EE7606F7449D5" modified="2013-10-16" version="1">QQHLPSPERLEWVIAPEIARRFTPVSKEANKMIDNLDFFVYRFKPYGKSFIKSCQVSPDVYIQLALQLAYFKLYGHLVTTYESASTRRFLLGRVDCIRSASTEALEWAKAMCQDEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUW6</accession>
    <name>T1GUW6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein phosphatase methylesterase-1</fullName>
            <ecNumber evidence="2">3.1.1.89</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48548"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="3.1.1.89"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02122694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02122695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02122696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUW6"/>
    <dbReference type="STRING" id="36166.T1GUW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007538-RA">
        <property type="protein sequence ID" value="MESCA007538-PA"/>
        <property type="gene ID" value="MESCA007538"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1442610_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="6">
        <location>
            <begin position="54"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007538-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="20990" checksum="6DC8ED4671145C4E" modified="2013-10-16" version="1">MGEQRDSFKKSRGRDFKPTVWSEFFSQKEVIVLDEKTSFCVYKSKPPEKEGPIILLLHGGGYSGLTWAQFSIEITSMIHCQCLSVDLRGHGETITEDDLDLSADTLSSDVGRLIEKMFPENCPRIHLVGHSMGGAIAVHIAHRLGKHDKRLGIIALDKCRFCNDLACGEDMVHLLNGWLHRGSPPFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVF0</accession>
    <name>T1GVF0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007749-RA">
        <property type="protein sequence ID" value="MESCA007749-PA"/>
        <property type="gene ID" value="MESCA007749"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3410961_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007749-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="29" mass="3213" checksum="A69CE79B71B03368" modified="2013-10-16" version="1">MPNLGIQYSNNERESDAKMVVEALTPFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVR5</accession>
    <name>T1GVR5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007881-RA">
        <property type="protein sequence ID" value="MESCA007881-PA"/>
        <property type="gene ID" value="MESCA007881"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1469852_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007881-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="184" mass="20268" checksum="AFC9415C9C56D891" modified="2013-10-16" version="1">MRSTSYIPNSSLPPPPKPIGGSMRITDQVIEEVDEQLTISQMTQSRKDFKDNAVQKGGPGSIYLRSYSSVSGGLTSPLESPNINESVSIGKDDMSRATVEDDVDDDDFEKLRKTRELKRHEKAQLKLARSISTHPGVLGDGDPRSSTIFSSEPINPALFRRNDDCEKISTGNNRKSIKSDINIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW42</accession>
    <name>T1GW42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02068317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW42"/>
    <dbReference type="STRING" id="36166.T1GW42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008013-RA">
        <property type="protein sequence ID" value="MESCA008013-PA"/>
        <property type="gene ID" value="MESCA008013"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_354369_0_0_1"/>
    <dbReference type="OMA" id="HEAHEVE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000184">
        <property type="entry name" value="Ecdysone-inducible gene E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39069">
        <property type="entry name" value="ECDYSONE-INDUCIBLE GENE E1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39069:SF1">
        <property type="entry name" value="ECDYSONE-INDUCIBLE GENE E1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="421"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="555"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="652"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="677"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="684"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="743"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="755"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008013-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="793" mass="89776" checksum="CB596B287AC9E8BB" modified="2013-10-16" version="1">NDDVVDDDVAFAAAAADDASEEVKTTEIPSVSSSSTSSSPLDEEHDEIVENEKEQESNLSVATSNIIDEKESETETEKPVFHSDYLDVESFEAIQSNKNDNEIETLNDGKEEEIESTTKTIVEYVTSHKVLEGNGSVDDSVEDVESDETATEFMKSEEHVPKLTDEEVYHEKEEHTTQPIEESVTEHITEKAPEFDDSEMLAQLTENLIEENPVKSESHVEQEPVKFESDVDVEDKEPVKTEKVEESSAMDEEPVKTEKFEETIAMDEKPIKAEPVQVQDLAEPEYITSSPPALEEEPIQQEIPEEPIKHELEEEVPSKQHSEEDQEKEEEPTEEHQPEPEFEPIDQIIPEPIASDNLVVEQPQTTASTVAEQPELSSPQPTASKEEESNESQESIFNPFASGQNIINQISDQLVSEVNEIVEQFGSSEESQDDKEEEPVKYEKQEEEPAKGSEEKQEDDKEEEPAKASEEKQEDDKEEEPAKASEEKQEDDKEEEPVKTSEYKKQEEEPFKASEERQEEEPLKVSEEKQENDKEEEPVKAFEEKQEDDKEQEEEPFKASEEKPEDDKEQEEEPFKASEEKQEDEENLEKIGGGCRSSGVCISWFFVCDGRPDCSDASDEECTFNTRRNQTCPEQSFLCERSGRCISRAARCDGRRQCPHGEDELECNSTFSKSGTCPPNTFRCNSGECLPEYEYCNAIISCRDGSDEPPHLCGSRYVPQMFLRLLAGAESTSELNTYCPHRCSNGRCRSTAIVCSGRDGCGDGTDEETCSVCSKYLISIYYFRNFDTFLVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWI2</accession>
    <name>T1GWI2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008159-RA">
        <property type="protein sequence ID" value="MESCA008159-PA"/>
        <property type="gene ID" value="MESCA008159"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782111_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008159-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="16122" checksum="E2C1EC7A5CE308BC" modified="2013-10-16" version="1">KDWTLHNLAELEEPLSICLQSEAQIDSSSPDGTSNMNTAQNDFSDLPKYYPLEDSLMGRLGGNNPMSPISQDYQSLYKVQQQQHHQQQALPPDLNDISLYGLDNENLSPPTSQSPQYPNSSNSTTIQTTIPSIVFTDCSGGGDFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWK8</accession>
    <name>T1GWK8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120796">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008185-RA">
        <property type="protein sequence ID" value="MESCA008185-PA"/>
        <property type="gene ID" value="MESCA008185"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880595_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008185-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15014" checksum="FA43114FFFA7E5F8" modified="2013-10-16" version="1">FCVLSDGTRSGTCTHITKCSGGHFGPNDPLSYTKCSTGFLMICCPLGFDKTLTSTEAPTTTKASTTTFEESSIHFKTGTLTISAQKQFGANDGAPLSAAEFLKATFFFLNKTFHLLLWKIYMMKRSQKMYVPQKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU2</accession>
    <name>T1GWU2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02198477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWU2"/>
    <dbReference type="STRING" id="36166.T1GWU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008273-RA">
        <property type="protein sequence ID" value="MESCA008273-PA"/>
        <property type="gene ID" value="MESCA008273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146247_0_0_1"/>
    <dbReference type="OMA" id="VKTLHEX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17748" checksum="98604F4402E61D3B" modified="2013-10-16" version="1">MATDISMQQTESFGETTPLPPQAATHNQLEQFVILSKKVKGKACLELIKQVLESPGVHVFGELLSMPNIKELEQSPDKKYLNTLNLFAYGTYKDYTKNPSEFLELSPVMKKKLQHLTIVAMAIKSKCIPYSELLKELEMTNVRALEDLIIEAIYAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX72</accession>
    <name>T1GX72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 170A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM170 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02116626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008414-RA">
        <property type="protein sequence ID" value="MESCA008414-PA"/>
        <property type="gene ID" value="MESCA008414"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149050_0_0_1"/>
    <dbReference type="OMA" id="VEMWYHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF6">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008414-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15482" checksum="E5D96FD954656E5A" modified="2013-10-16" version="1">MGLQYKTLSSFRAMWYHIFVWTLFSSIFIHTCAAVFAFVTLRKHKFGRFFSILILVMGVVFPVTSGAVSSACIAFVHRASSIQINAIYAMIWGVGQTILSACLGFTRILATLLTTFLQILDLFFCTEQVPNKPQQENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXF6</accession>
    <name>T1GXF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXF6"/>
    <dbReference type="STRING" id="36166.T1GXF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008506-RA">
        <property type="protein sequence ID" value="MESCA008506-PA"/>
        <property type="gene ID" value="MESCA008506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_8_1"/>
    <dbReference type="OMA" id="VANRIMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="34052" checksum="54E436C20EAFADE6" modified="2013-10-16" version="1">MSLTMLSVALIAIAAYLIYLYFTRNFDYWKKRGVKGPKPKLIFGNFKDSITEKRNVGYLIDDIYRNYKKSEDFVGVFNANFPQLLILNPELAKQITTSDFSSFADNEASEWTKSSKDLLIRYHPFVLTGQEWKNRRSELIPSVTPAKIKSYYPNHVDATDLMLKYTGEVVCDVVFGLEAGTFKTETCDFMKVSRKLVSDFYNELGKLFISSCFPILRGVFKINFIKDDTANFYRNISKLAIESRLKGNDRNDVLQFLINMQEKNNISHDQFVGNQLMFILDGYDTTAVITAHVLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXF9</accession>
    <name>T1GXF9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXF9"/>
    <dbReference type="STRING" id="36166.T1GXF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008509-RA">
        <property type="protein sequence ID" value="MESCA008509-PA"/>
        <property type="gene ID" value="MESCA008509"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429565_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008509-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10511" checksum="4AB98318314C35DC" modified="2013-10-16" version="1">MSSFTFGLILTQLEKYHFETDLQGSITPINFWATKTDKPESEIVIKETYNKTIRIMEFLQNYFSAKLPLSKVDLVGVPTFSPMRPADNWGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXP4</accession>
    <name>T1GXP4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXP4"/>
    <dbReference type="STRING" id="36166.T1GXP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008597-RA">
        <property type="protein sequence ID" value="MESCA008597-PA"/>
        <property type="gene ID" value="MESCA008597"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654147_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18338" checksum="EC65A48ACE2C5D94" modified="2013-10-16" version="1">MDSCATVSPSAFEPVPGWVDNLNGPTGLMVGAAKGVIRSIPPTCPVYTITISERRKLTWGEALVMSKRVGYKYPVLMGLWYPDGSVTMNKMKHRMNVFFFQWIPAYLVDFLMWILRKKPLFNQDMDAVESPEDYMTTCITGGRQYILKEGWIHFLERDCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXT3</accession>
    <name>T1GXT3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXT3"/>
    <dbReference type="STRING" id="36166.T1GXT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008638-RA">
        <property type="protein sequence ID" value="MESCA008638-PA"/>
        <property type="gene ID" value="MESCA008638"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375148_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008638-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10093" checksum="5CB45042F28184EA" modified="2013-10-16" version="1">MAVYCNISNPNMWPEDVGLDVIHESFDFIVVGAGSAGSVVGARLSLNSNYKVLVIEAGGGGCSIVNYIKGESLAINEAKLIFQLSKFRSPDKISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H083</accession>
    <name>T1H083_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H083"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009556-RA">
        <property type="protein sequence ID" value="MESCA009556-PA"/>
        <property type="gene ID" value="MESCA009556"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388703_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009556-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="11026" checksum="F8BCA06BA7164805" modified="2013-10-16" version="1">MIVQVYGRFYLIRNSSLSSKFTHDCDEANYNFVDVLQETSISEFFTKFMMNYCNPVRSGMEKSTQIYQEDKSKALQRACHASTDLWITVLDEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H091</accession>
    <name>T1H091_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H091"/>
    <dbReference type="STRING" id="36166.T1H091"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009564-RA">
        <property type="protein sequence ID" value="MESCA009564-PA"/>
        <property type="gene ID" value="MESCA009564"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944351_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009564-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6760" checksum="5040FC147942568A" modified="2013-10-16" version="1">MNNFKLTDKVVLLMGKSGSGKTSMRSIIFANYIARDTSRLGATKRQYISPNVPICFRKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1F0</accession>
    <name>T1H1F0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1F0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010009-RA">
        <property type="protein sequence ID" value="MESCA010009-PA"/>
        <property type="gene ID" value="MESCA010009"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760711_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010009-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7635" checksum="2C466B0D782650DE" modified="2013-10-16" version="1">MLTLERVNSSGSSVNTKSIRDIGIKQLSSLENIHSTSGKLKTVSESLLSSQLSYELVTLVTPNCNLRNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1K7</accession>
    <name>T1H1K7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190339">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1K7"/>
    <dbReference type="STRING" id="36166.T1H1K7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010070-RA">
        <property type="protein sequence ID" value="MESCA010070-PA"/>
        <property type="gene ID" value="MESCA010070"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1088080_0_0_1"/>
    <dbReference type="OMA" id="VEVATCD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000098">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000118">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF328">
        <property type="entry name" value="CADHERIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="12"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="101"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010070-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="256" mass="29007" checksum="5F26348F9EC3E118" modified="2013-10-16" version="1">MPVTFIDDVENTVFDHDVGRNGTFQLFIDPPTDTFEIIPEVAVNEATFIIRVKDPLLLDYEMQTEVNFTIIAKEVESPGKWSAAHVQVFIVDQNDNYPEFTQSVYNVTIPENSEVGSFITQIKAYDKDSDDFGTMGIRYKNLRGSIAHLLTLDPVVGTLSIKSTNSEAFDREIVSRYYLTVEAIDNNGLGNRNTAQIIIDLEDVNDNAPIFLQHQYEANLLENKDDFENPLILEARDLDLEDTENSQITYEIIDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Q0</accession>
    <name>T1H1Q0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Q0"/>
    <dbReference type="STRING" id="36166.T1H1Q0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010117-RA">
        <property type="protein sequence ID" value="MESCA010117-PA"/>
        <property type="gene ID" value="MESCA010117"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485918_0_0_1"/>
    <dbReference type="OMA" id="AYAKICI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010117-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9767" checksum="BAF62598FDD211B6" modified="2013-10-16" version="1">MIFPDPENIVDKSPSSPTRRKLPKTPEQIAREKQVSGRVQLQVWYHGEKNELVVSLMGADNLAIRDDTWGHGTLPEAYAKICIVPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1S3</accession>
    <name>T1H1S3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1S3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010141-RA">
        <property type="protein sequence ID" value="MESCA010141-PA"/>
        <property type="gene ID" value="MESCA010141"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111648_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010141-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12650" checksum="2ADB2259245EE40C" modified="2013-10-16" version="1">MVAEDDFPCINMSSASIPMCCTSSINITATFPTPTVTEAANSFCFALGRHGFRYPSISGDATCKSYVYCYLYAGRMRGQQYTCPGRTVFDCRTRACVVPTVPLQDCTFCNPQCFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Y2</accession>
    <name>T1H1Y2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Y2"/>
    <dbReference type="STRING" id="36166.T1H1Y2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010206-RA">
        <property type="protein sequence ID" value="MESCA010206-PA"/>
        <property type="gene ID" value="MESCA010206"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944341_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031937">
        <property type="entry name" value="PNISR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31518">
        <property type="entry name" value="ARGININE/SERINE-RICH PROTEIN PNISR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15996">
        <property type="entry name" value="PNISR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010206-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6848" checksum="8A4E90A11FD47580" modified="2013-10-16" version="1">MLVVRRSLTELLLEVTNEEIQSIAQEQYKKHKSKGTSSSVQVFHKSALKRITGNLGKFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1H219</accession>
    <name>T1H219_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NR LBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H219"/>
    <dbReference type="STRING" id="36166.T1H219"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010249-RA">
        <property type="protein sequence ID" value="MESCA010249-PA"/>
        <property type="gene ID" value="MESCA010249"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755620_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NR LBD" evidence="7">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="258"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010249-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="367" mass="40231" checksum="1F4FBD7C623312B7" modified="2013-10-16" version="1">MVSWTKKLPFYLEIPVEIHTKLLTDKWHELLILTTAAYQALHGKKSHSSPSTNLTSSSSTTTTNTTNIFGTTNISNDDPDFTQEVSAHLSTLQTCLTTLMGQPITMEQLKLDVGHMVEKMTQISIMFRRIKLRMEEYVCLKVYILLNKGIMTLSRGPYSELDKMSLFQDLKLKRRKIDSRCSSDGESIADTSTSSPDLLAPLSPKMCDEETRVTITSISSASTPVTSSLLIPTTNQTTQSQSQQQQQQQLQNKNIIQQVAASTSSSDMSSACKKMMSSLDKNASSIPSSSSSSQRSENDVNNEHDITSGAEEGRSLDSVDNKPSTTTSAISTNGIYSVRSVDQSDKSDRSDLSSSTEPSSGGQTTNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2E1</accession>
    <name>T1H2E1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
            <ecNumber evidence="6">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02107770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2E1"/>
    <dbReference type="STRING" id="36166.T1H2E1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010383-RA">
        <property type="protein sequence ID" value="MESCA010383-PA"/>
        <property type="gene ID" value="MESCA010383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1742634_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF43">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="30"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="17424" checksum="91EE30FB2573E423" modified="2013-10-16" version="1">MSAYAWKSTTNQLFRFYLYQLDLSESSHAQWNQSRCVDSIRARIQKEIFASYQYLAMAAYFSRDTVNRPGFTEHFFGASKEDALHLIEYLSMRRNLTSVIINLITNTQVKKAEWINAVEALTDALELNAELTKSIRGNWKGSQMQRLPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2H6</accession>
    <name>T1H2H6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2H6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010422-RA">
        <property type="protein sequence ID" value="MESCA010422-PA"/>
        <property type="gene ID" value="MESCA010422"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856328_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010422-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7546" checksum="3805A88F3FF4A2B6" modified="2013-10-16" version="1">NQFPTNGIIESFSYPQKGENCENNVFVVELIPDNRGSMNWKNQYSNVFLNGTYKPSQTLLRIYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2R3</accession>
    <name>T1H2R3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        <source>
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</entry>
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</entry>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01854">
        <property type="entry name" value="BR22PROTEIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012044-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="8319" checksum="17284B1DA6B25DDF" modified="2013-10-16" version="1">KKIEQKRSNEILKKKEGRTCRSFRSKTKKLAKQKRLERTKMISKRTKKGQPLMKDRIEFLLKQIQDSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5V9</accession>
    <name>T1H5V9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5V9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012092-RA">
        <property type="protein sequence ID" value="MESCA012092-PA"/>
        <property type="gene ID" value="MESCA012092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3432101_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="17" mass="2205" checksum="3E584C24B5917232" modified="2013-10-16" version="1">MMMMAKKIKRNEVYFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5X4</accession>
    <name>T1H5X4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein bicaudal D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BicD family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5X4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012107-RA">
        <property type="protein sequence ID" value="MESCA012107-PA"/>
        <property type="gene ID" value="MESCA012107"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014107_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072393">
        <property type="term" value="P:microtubule anchoring at microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018477">
        <property type="entry name" value="BICD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233">
        <property type="entry name" value="BICAUDAL D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233:SF6">
        <property type="entry name" value="PROTEIN BICAUDAL D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09730">
        <property type="entry name" value="BicD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="31"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="240"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="520"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012107-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="75157" checksum="D2858422E5AED799" modified="2013-10-16" version="1">ALAKYQTSQKVTTETGIESEQSLLSESAAIETSLNLHILDLENEVKTLKQELDRVKNERDALQENTDMGQNKTSAGNEVLNSAMNDYKRRETRMLSDYSELEEENITLQKQVSSLKSSQVEFEGAKHEIRQLTEEIELLNSQVEELSNLKKIAEKQMEDALEALQGEREAKYALKKELDSHINRESMYNISNLAYSIRGMEDNNSSTNSDGEEDNIALKRLEADLTDLKSPDGNKVDLFSEIHLNELKKLEKQLEALENEKTHMTTNLRDAQSNLDKSQNELQNFMSRISILTAHVDALVQLKKQIDSNRDEPKTKDEHDAINEKLKCPYQYSNWFSISSKEIDGLKNDLAELQKGLNYSDPMVTLRNEITNLKNKLHSSEQKSLDLTSDVQTLTNISQNAGQSLGSARSTLVALSDELAQLYHLVCTVNGEQPTRVLLDHKTDDMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKYNREEVEELQEQIIKLKSLLSTKREQIATLRTVLKSNKQTAEMALTNLKSKYENEKLVVSETMSKLRNELKILKEDAATFSSLRAMFAARCEEYVTQVDDLNRQLEAAEEEKKTLNQLLRLAVQQKLTLTQRLEEMEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1H608</accession>
    <name>T1H608_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H608"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012141-RA">
        <property type="protein sequence ID" value="MESCA012141-PA"/>
        <property type="gene ID" value="MESCA012141"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848078_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012141-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="328" mass="37043" checksum="CF4D1B75EDDA7A02" modified="2013-10-16" version="1">MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPSLLGQKIEVKIPTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIELLETDTKKKWTRPPISMNFEVPFAPSGFKVRYLKVFEPKLNYSDHDVVKWVRYIGRSGLYETRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6F0</accession>
    <name>T1H6F0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide. Man(5)GlcNAc(2)-PP-dolichol is a substrate for ALG3, the following enzyme in the biosynthetic pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="T1H6F0"/>
    <dbReference type="STRING" id="36166.T1H6F0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012283-RA">
        <property type="protein sequence ID" value="MESCA012283-PA"/>
        <property type="gene ID" value="MESCA012283"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017896_2_0_1"/>
    <dbReference type="OMA" id="ARLYGWV"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000227">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="16">
        <location>
            <begin position="2"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="15">
        <location>
            <begin position="232"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012283-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="406" mass="46465" checksum="27384C4AC2ADE442" modified="2013-10-16" version="1">VNVGIFHPYANAGGGGERVLWCAIRALQAKYDNVKIIIYSGDIEADPNTILKRAKKVFNIEVDEEKTSIVYLKKRYWVEAKRYNYFTLLGQSLGSMVLGFEALRKFPPDVCLDTMGYAFTYPIFKYLGECKVGCYTHYPTISTDMLKRVQNRVQSHNNRRAVARNPFLTWIKLAYYKIFARIYGIVGRCADTIMVNSSWTEGHIEDLWDCQFKIHRVFPPCDVGHLKQLKYTGNDEEKIIILSIGQFRPEKDHPLQLQAMYELRTLLSQNEDLWEKLHLLIVGSCRNEEDEELLKNMQDLSKHLSLENNVEFKNVPYKELIELYQSAKIGIHTMWNEHFGIGVVESMAAGLIMVAHRSGGPDMDIVETSEGSQNGYLATDAVEYANCILHILCNSDEQNEAIKNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6J1</accession>
    <name>T1H6J1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6J1"/>
    <dbReference type="STRING" id="36166.T1H6J1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012324-RA">
        <property type="protein sequence ID" value="MESCA012324-PA"/>
        <property type="gene ID" value="MESCA012324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071681_5_2_1"/>
    <dbReference type="OMA" id="QGILNQP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1340:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="13170" checksum="B00AD9910F730150" modified="2013-10-16" version="1">TLETEEQQRLRFQIELEFVQCLANPNYLNFLAQRGFFKDQSFINYLKYLQYWKEPEYVKFLMYPMCLYFLDLLQYEHFRREIVNAQCSKFIDDQAVLLWQHYTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H724</accession>
    <name>T1H724_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDB1- and CUL4-associated factor 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">WD repeat and SOF domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DCAF13/WDSOF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H724"/>
    <dbReference type="STRING" id="36166.T1H724"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012506-RA">
        <property type="protein sequence ID" value="MESCA012506-PA"/>
        <property type="gene ID" value="MESCA012506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033999_0_0_1"/>
    <dbReference type="OMA" id="EDHNAYI"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000132">
        <property type="entry name" value="DDB1- and CUL4-associated factor 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000826">
        <property type="entry name" value="DDB1- and CUL4-associated factor 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007287">
        <property type="entry name" value="Sof1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051733">
        <property type="entry name" value="WD_repeat_DCAF13/WDSOF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851:SF0">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04158">
        <property type="entry name" value="Sof1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="320"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Sof1-like protein" evidence="11">
        <location>
            <begin position="353"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="444" mass="51546" checksum="4CA6EF69C56BD82F" modified="2013-10-16" version="1">MKVKIISRNPDDYMRETKNELHKIKRNYDPDLHPMESTREYVRALNATKLERVFAKPFVGNLDGHRDGVAVFGKHPSHLSALCSGAYNGEVRVWDLPTRQSIRNFIAHDGFVRGICYTAKGDRFITIGDDKAIKFWNAEKPEFEEDEEPINTILSRTSIHGISHHRKNNIYATSGEVCYIWDETRSEPLKTLKWGVDTLHSISFNQVETSLLAACASDRSIIFYDQREVKPLRKVILTMKSNKLAWNPQQAFNFTVANEDFNLYTFDTRQLKNPIKIHYDHVSAVTDVDYSPTGKEVTGSYDKTVRIYEVHKSHSREIYHTKRMQRVVCVGWSYDSKYVYSGSDEMNIRLWKAKAHEKLGVARPREKANFNYQEALKEKYAAHPQIKKIARHRHVPRHVYNAQNKMRTMKAKEQKKEGNVRKHSKPGTVPYVPAKHKTVIKEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H736</accession>
    <name>T1H736_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H736"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012518-RA">
        <property type="protein sequence ID" value="MESCA012518-PA"/>
        <property type="gene ID" value="MESCA012518"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369288_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029270">
        <property type="entry name" value="LIX1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14954">
        <property type="entry name" value="LIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012518-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="22222" checksum="9B46A56F38638B96" modified="2013-10-16" version="1">YPEEPFWASPQISGLYDDGDYRVNVVEALQEFWQMKQSRGTELKNGAVVIYESVPSNSQPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDEFIEKAVQEARTSFKGDTTSNDGSDAGIGAFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7A6</accession>
    <name>T1H7A6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7A6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012644-RA">
        <property type="protein sequence ID" value="MESCA012644-PA"/>
        <property type="gene ID" value="MESCA012644"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122455_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="115"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012644-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="248" mass="27219" checksum="8D0BD898CBDB1D3E" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVFLGGVDKNTQFVRYFLGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGGQREFNGLIDCLGKISKSDGIVGLYRGFGVSVQGIIIYRAAYFGFYDTARGMLPDPKNTPIYISWAIAQVVTTVAGIVSYPFDTVRRRMMMQSGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7B6</accession>
    <name>T1H7B6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H7B6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012654-RA">
        <property type="protein sequence ID" value="MESCA012654-PA"/>
        <property type="gene ID" value="MESCA012654"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011007_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:double-stranded RNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16638">
        <property type="entry name" value="mRING-HC-C3HC3D_Roquin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041523">
        <property type="entry name" value="ROQ_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052249">
        <property type="entry name" value="Roquin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139">
        <property type="entry name" value="RING FINGER AND CCCH-TYPE ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139:SF54">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18386">
        <property type="entry name" value="ROQ_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012654-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="426" mass="48014" checksum="335889D6DA6B3DF5" modified="2013-10-16" version="1">MPIQAPQWTEFLYCPVCCNEFAAVNRPPISLGCGHTICRACLATLQNKLCPFDQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDSSLMQLKEEFRTYDALRREHDAQIVQIATEAGLRIAPDQWSSLLYGDPAHKSHMQSIIDKLQTPQSFNQSIQELVIAIQRTGDPANLSNLRTYFKRLAGIDASLEVTVPTFEQVANALECLKYVVFGLLEFVQHHGNRKLQISNFLTNKLNPKYKISLCRDLTLRRCPRGTSCTFAHSEEELEKFRAKSRKNSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7L8</accession>
    <name>T1H7L8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4773 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7L8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012755-RA">
        <property type="protein sequence ID" value="MESCA012755-PA"/>
        <property type="gene ID" value="MESCA012755"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2339512_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012755-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11291" checksum="24F257E877AD1DF0" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKHYCLEILDDGEDVFVESFQSEELPEDLDIQICSNKSSYCNDLPIQEDYNLEDDEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA33</accession>
    <name>T1GA33_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Guanine nucleotide-binding protein-like 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151510">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA33"/>
    <dbReference type="STRING" id="36166.T1GA33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000078-RA">
        <property type="protein sequence ID" value="MESCA000078-PA"/>
        <property type="gene ID" value="MESCA000078"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1327737_0_0_1"/>
    <dbReference type="OMA" id="HKGRCAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanine nucleotide-binding protein-like 3 N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000078-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="221" mass="25835" checksum="3DFEC0219964A4E7" modified="2013-10-16" version="1">MALKRLKTKKSKRLPASHKFKIQKKVREHHRKLKKEARKNGKKGGSKKQKLIQIPNICPFKDEILKEVNDYKEHQQNERIKQRQNQYKDLHSLVADAEEAGTSFAQKVDIEVKEKQSNTEKNDNSLKVFYKEFNKVIQDADVILEVVDARDPIGTRCEEVSKTVKSLPGNKKLVLILNKADLIPLENLKSWIIYSGNLDQLLDLKHQHRIKTKTWEEKELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA47</accession>
    <name>T1GA47_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA47"/>
    <dbReference type="STRING" id="36166.T1GA47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000092-RA">
        <property type="protein sequence ID" value="MESCA000092-PA"/>
        <property type="gene ID" value="MESCA000092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1334822_0_0_1"/>
    <dbReference type="OMA" id="VHPMVSI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF6">
        <property type="entry name" value="CONTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="17"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="116"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="206" mass="23291" checksum="3F0B682379DAC28E" modified="2013-10-16" version="1">GFIFGDLNFNNDLEYNKKFESKPTTVKTFENDTVLLPCYPKNSPVRYVRWHRDDKLLADSRHPGNPPPERVYLWPNGSLQVSHVKTEDTGEYYCEMMTTANNHAVQTHAIEVQYAPSIVMSRSGVQEVPIRAVFELVCEAKGVPHPVISWRKNGMELENEKVGNRRVLTIEIKSREDSGTIECIATNGVGTPAVDTLHVNVLCKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAC0</accession>
    <name>T1GAC0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000174-RA">
        <property type="protein sequence ID" value="MESCA000174-PA"/>
        <property type="gene ID" value="MESCA000174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253106_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2080.10">
        <property type="entry name" value="Insect odorant-binding protein A10/Ejaculatory bulb-specific protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005055">
        <property type="entry name" value="A10/PebIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036682">
        <property type="entry name" value="OS_D_A10/PebIII_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11257">
        <property type="entry name" value="CHEMOSENSORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11257:SF13">
        <property type="entry name" value="GEO07322P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03392">
        <property type="entry name" value="OS-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100910">
        <property type="entry name" value="Chemosensory protein Csp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576757" evidence="1">
        <location>
            <begin position="20"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11538" checksum="50324FC27FD26905" modified="2013-10-16" version="1">MKFSLALLALACVFGIISAEKAAYTTKYDNVDVDSILSNKRVLDNYIKCLMDKGACTAEGRELKKEEKGPEIWPTTEGVVTFNCRAKRSLHRIVSLGKKCVLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAE0</accession>
    <name>T1GAE0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAE0"/>
    <dbReference type="STRING" id="36166.T1GAE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000195-RA">
        <property type="protein sequence ID" value="MESCA000195-PA"/>
        <property type="gene ID" value="MESCA000195"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2910784_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000195-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="7238" checksum="0F49BE7FB9C5D1B5" modified="2013-10-16" version="1">MSPFIISMEISGDEDRTKIAMLQSLGWSVSTTLLPLLCWWLQDWYPFMWVTTIPTILILIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAH1</accession>
    <name>T1GAH1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000228-RA">
        <property type="protein sequence ID" value="MESCA000228-PA"/>
        <property type="gene ID" value="MESCA000228"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161253_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF30">
        <property type="entry name" value="GH01206P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13512" checksum="B120BE23B3298916" modified="2013-10-16" version="1">MKRLKMKPPKITITTSENETIEEFSEESVPEGDDSLTSALSLLYGKFLIILGITLPITQLLTNQVPNPIYQSFYIYLYAISILFVLFIYGISICQSEEYSSILNLILKNFGILQFRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAS8</accession>
    <name>T1GAS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAS8"/>
    <dbReference type="STRING" id="36166.T1GAS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000339-RA">
        <property type="protein sequence ID" value="MESCA000339-PA"/>
        <property type="gene ID" value="MESCA000339"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010920_4_3_1"/>
    <dbReference type="OMA" id="LVCISWV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="2">
        <location>
            <begin position="2"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000339-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="25740" checksum="E0976DFC88F7956F" modified="2013-10-16" version="1">MMIVAISWVSFWLQADQSAPRIMLGTSTMLTFITLASSQGKTLPKVSYIKVSEIWFLGCTGFIFGSLVEFAFVNTIWRRKKNVELKKINSKYILKSTITPRLARKDMGLHKSRSCSSLDNNSSNQPSYSNHYLTVHNIPVITTNSDEEFAKEPNGHYKIDMETNDSSNNLSMADSTRSQENLTYNWTTMTPQEISIWIDRRARFVFPLAFIVFNALFWTFVYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB94</accession>
    <name>T1GB94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PseI/NeuA/B-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB94"/>
    <dbReference type="STRING" id="36166.T1GB94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000520-RA">
        <property type="protein sequence ID" value="MESCA000520-PA"/>
        <property type="gene ID" value="MESCA000520"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1205980_0_0_1"/>
    <dbReference type="OMA" id="HEVGINI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047444">
        <property type="term" value="F:N-acylneuraminate-9-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051690">
        <property type="entry name" value="PseI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="PseI/NeuA/B-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966">
        <property type="entry name" value="N-ACETYLNEURAMINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966:SF1">
        <property type="entry name" value="SIALIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseI/NeuA/B-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000520-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="25919" checksum="75BCB98DEFBC5BB6" modified="2013-10-16" version="1">MIAEAKRVGCDCVKFQKSDLTFKFTKFALERPYKSENSWGSSYGEHKTFLEFSIEQYKFLQSYAHSIGILFTASAMDIKSLEVLESLDVPFIKIGSGDANNFPLLKKAANLEVPLIISTGMQSLDTIKEIVKIMTDAKKRNYALMHCVSSYPTKPEDCNLKTITLLQDMFPSVPIGYSGHEVGINITRPPGPWTVHRRSMDQFGDLGKATEISSEKERNYQTLSVKEKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBI3</accession>
    <name>T1GBI3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02029600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBI3"/>
    <dbReference type="STRING" id="36166.T1GBI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000615-RA">
        <property type="protein sequence ID" value="MESCA000615-PA"/>
        <property type="gene ID" value="MESCA000615"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038704_0_0_1"/>
    <dbReference type="OMA" id="FQSESVW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000615-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="277" mass="31636" checksum="7D8ACBF6B0535799" modified="2013-10-16" version="1">NYRSSTIDWYPSVEVLPTEDHLTNLNLCALNSTSITSKDLEIHYVHSGSIIKIIDTGKPKEDDEDFKVVLSDIKSAESKHSDLLPGVYEGGAKIWECTIDMIHYLSENTKRSDYQEKRVLDLGCGSGLLGILALQCKAIVHFQDYNKDVLETLTIPNVLLNFDPEPTLEDIKDHKFYSGDWSKYVQLTRDDPKFDFIFTSETIYNPINQQKLLDTFADKLNKSGTVYLGAKIHYFGVGGGIRQFEELLKKDNRFKTKVVWTSSEGVQREILKINWND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCM3</accession>
    <name>T1GCM3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GCM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001041-RA">
        <property type="protein sequence ID" value="MESCA001041-PA"/>
        <property type="gene ID" value="MESCA001041"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_238982_0_0_1"/>
    <dbReference type="OMA" id="KPFVNWA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36812:SF9">
        <property type="entry name" value="MYB-LIKE PROTEIN X ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36812">
        <property type="entry name" value="NEUROFILAMENT TRIPLET M PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="528"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="632"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1398"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1462"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="480"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1059"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1070"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1102"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1160"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1265"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1306"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1398"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1421"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1463"/>
            <end position="1479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001041-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1765" mass="198607" checksum="DD0BD8D873938F00" modified="2013-10-16" version="1">MVAETNHTRSRSEVLSCTFDINFYDTIASNVRKNLSSFVDGKLEEGQSLVINPTPTIRSTLVRSPVHINSDGEIHKATSLSIVTAFPTLLTADQYNGCDSLSSKKRSHTKNSWKWKWDSVKKYKYVNEGGKIVKKVKHGCQGLRDLSKLDMWTQLTMRTKFEKHLNLEEKTEERRQIDELNFILNHRRLPDISLEQSEQSFIKLERSDFNEIDGVEEIQRPSENEGTDELDFPMELNLSRRTTQTLGSLGEWARPRFYVCFGCGEKFEKIKGLEEHKYHKHPYVASTHYEMVGRELLQDDLYKNFFVPLKALQMLRENGLNTNKLKTDCTQRNDHHDESMDSVHSVIPIEGGTASDDSDSKTTALTVHSNITRSTDELDNINYLVEQQEHRTTSGNSLPENGLECSKCNKICHGSLELYKHILDCSGDYAWILAKRKKYRYIGTSRRRKFNRNSNSTKKKKDATDSATKSERQNNEDNRSVTQSPKPCKSMNSAQKNKQTDAETIQRMLENLPAKRVCRQILPQLNQKPKNKKVEEKSCSSSKAIVNEPEIPSAVSEEKEENKSVKNTQPAEKAIEEIVVNNGNIAIITENKKSSKSTVKSVKKVNQKSVAKDAVDEQIVDETSLIDIKEDESKKKLRSSPRNQSNVEVSEEKQTSSITTTATTTTPTATTTSTRRPKKINDCIAKLQEKLGLPFFGSPTSSNKANDPSLTLNEIKTPEKIDISKHVTAVTEVNTSPVKEILNKSSEIDDTKLVKTPKRKSLTRKIVKTDISIPSLPSKKIEQPQQQMTAITPIKPIQIQTVHPTIQHQPVTQQHQHFSQKPIAHVEAIRPKHFIDTNLPPRRPSILLPTLVPPPPPPPILLPSTVTLPLPQDLLLDAPLDLSRKPVEIKTHTIADILQNSQAKRRSKSSKKEVVPVPPIPISSPITQSLPIVNELKNKLPNVSITSKCVLNIPQTPAFDKGVETLKKQIKENTQLVIPNFKGCVDIIKIPAPKPPIIHAEKPSVTIKAIPPPPPKIIDKPQDKGFISAFESFIEKKAADNSIPPPPLGKDSEASIKEKKPRARKAPKKAKAEELKKPPPEKDVKGNDENKSPTNKRRSNRIKVDPKNIEAEKAKEKLLEKDTVEEKEKPMEVPQDSPKKDPIEMEKPLEISEVVPVEPQIEKEIPNEIPEENHEEIQKENDNCVEKEKSKETPENKRKCKANLEEKEKRLEFSKAKGPKHKEVKVNVDEKEQSKGKTKDAKGNVEDKEQAIEISKPKGKSKERKSKDVVDEIEKTKENPKAKGKSKDIPKDIEKSKPKGKLRGSNGDEKETSIEKEISKETPLEKEKSDVKEKPKGKPRKPIKEKLKQKKVLEVKKIIEEEEKPKKTSIEKENHIEKEILSDEDVPLSVTKASLVAESSKNGAKLQTENQSKTTQPIEIEVSKPKDPESSKKKKKILEQTEQESEIPKVTVVVREVPAEPFNPTNTEALHTSEQNFESESTRLKLNPDEKLFYECCNILKGSETPRVERTYPITNTTYTYDIPRPPEVRKNLPADGNQFSDISSDTPNVDNRNVVSARIVVNQPYGGQPSNFRSPIHSASSQGSVAAAPVFQSTNCRLKTKAAMKGYDNLKVSIPTYGLDLQQALERSPQGGCKLTALADIALGSEASKAAIPIDNDSDAIDTPLVEEKAKTPEYLSVSGTQKQKDVYDFDDTIDNMSDKVAHIQPKPTKSTKIVIPDRPFKNIGRDEETTIKFQKPEVVVQQNEESLLSTLSYSDRDDFNMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCY8</accession>
    <name>T1GCY8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001162-RA">
        <property type="protein sequence ID" value="MESCA001162-PA"/>
        <property type="gene ID" value="MESCA001162"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888313_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001162-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7518" checksum="0444A345FEF958EC" modified="2013-10-16" version="1">MIFWVICRTDIKGNDLADMVDKTRLGTALVVVNRKIHEIVPRPLKWLGEEDFNFVLRMKRKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD72</accession>
    <name>T1GD72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock protein 83</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02099379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD72"/>
    <dbReference type="STRING" id="36166.T1GD72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001253-RA">
        <property type="protein sequence ID" value="MESCA001253-PA"/>
        <property type="gene ID" value="MESCA001253"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006684_3_1_1"/>
    <dbReference type="OMA" id="HEFCVES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000004">
        <property type="entry name" value="Heat shock protein 75 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000004">
        <property type="entry name" value="Heat shock protein 75 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000004">
        <property type="entry name" value="Heat shock protein 75 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001253-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="56228" checksum="647901294D6F5C6C" modified="2013-10-16" version="1">MLQNCACFVTQRQFGVGFYSAFMVANRVDVYTKSSIKGSPGLKWSSDGSGSYEIQEADGVSIGTKIVIHLKPECREYSDEDRIQAVIKKYSNFVGSPVFLNGKKANDIKPLWLMEPKDVTKEQHDEFYRFVGQTFDTPRFVLHYKTDVPLSIRALLYFPEGKPGLFEMSRDGEIGVALYTRKILIQSKTENLLPKWLRFVKGVVDSEDIPLNLSRELLQNNRAQKQPSEYDHFYKDYGMFLKEGIVTGEDHKQKEEIAKLLRFESSKEAGQKISLPEYCNRVGEEQKDIYYLAAPNRVLAESSPYYESLKKKGTEVLFCYEPYDELVLMQLGMFLGKNLSSVEKEMRKDDKSDDMTDLGADSLKRSEITDLLAWMKESLSGKIANAKITNRLESHPCVVTVEEMAAARHFIRTQTHQLPEENRFALLQPQMEINPKHPIIKKLYSLKSSDPELAKLLVQQLFANAMVGAGLADDPRTLLTTINDLLTKVLEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDF5</accession>
    <name>T1GDF5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02059706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDF5"/>
    <dbReference type="STRING" id="36166.T1GDF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001341-RA">
        <property type="protein sequence ID" value="MESCA001341-PA"/>
        <property type="gene ID" value="MESCA001341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_11_1_1"/>
    <dbReference type="OMA" id="LLMFMSC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF46">
        <property type="entry name" value="FI02923P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="400" mass="44684" checksum="365F6FB6BCE58E9D" modified="2013-10-16" version="1">MYSIPRKQITGINIHKINVIVCSICVIYTMFGGIKAVVWTDVVQGFIMLASVVVVASFGIYKVGGFQQSSYTRQINNKHKLRFNNKINSWNCLISGLFLATSHIGFSQSCVQRLVSLPSIEQAQKSIYYFGFGLAVVVGFCNFTGLIMFANYVDCDPVRAGVVQKQDQMLPFFVQQTVGNIVGMSGFFISSVFSASLSTLSANLNSLSGIIYFDYIKRNVKHTEEKANFIMKIIVCLTGIYCIFGGLVVERFHSLIEVCVTWTGVAFGCLFGVFMMGFFVPKVHSKATFIAIVTSLIITIGIIVTGKIYMYLINYKYYPLPTSVEKCDALNINATMILLENIPKADPFDIWKMSFVWYSVVGSLLVWIIGIPLSYLMAPNKDEISNINLYAPIVKRILMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDS1</accession>
    <name>T1GDS1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase p58-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001467-RA">
        <property type="protein sequence ID" value="MESCA001467-PA"/>
        <property type="gene ID" value="MESCA001467"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212949_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase p58-like C-terminal" evidence="1">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001467-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12949" checksum="13E33FE3984F3D13" modified="2013-10-16" version="1">MFVKEKQQQIEDIHQFVRNKIQIVSDNMKARYDLKSNSAAFKDGELEFIQNYNQTGKVHKIIHKINDVVYRIRDEKNNRKRLKVVHLERLAPYKRTLKLNVQEEHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GED8</accession>
    <name>T1GED8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02017033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GED8"/>
    <dbReference type="STRING" id="36166.T1GED8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001700-RA">
        <property type="protein sequence ID" value="MESCA001700-PA"/>
        <property type="gene ID" value="MESCA001700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_642961_0_0_1"/>
    <dbReference type="OMA" id="ESYNICS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001700-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="427" mass="47966" checksum="417F0F415B8CCD7E" modified="2013-10-16" version="1">MEADQNEVNNIFDGDKYDIMTNDNPNCIIKRSKGLPGDPRNLSQMLDDKSHVNTVRHRYEEYSLISEEIDENDPEYNDEYDDSYEALLEGEIKTKKTEAMKQIIADIEDQESESEVEEEKPVENKMNFCENPEVIRARYEAARAAKMANKKGYRPPPVPRDVVGKAKGQGQEKDVLFNRHKKDVNKSSRANHNRKTGSSMERWQSKVAVVTGAAQGIGASCVKALVRAGVIAIESQMKFHTIKCDISKEAEVQNVFDQVKKQFGGPDILINNAGIVRPGEMVTPGNSKNIIDTVNTNILGTYFCTREAFLSMKERQVDGHVVNLNSTGGHTAYFDGESYNICSGTKHAITAMTETYRQEFQRNGNLVKVTSISPGNVGTEFLTEDMRKTMPMLHPDDVADAIMYALSTPKHVQIHELIIKPMGEKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEN8</accession>
    <name>T1GEN8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02119980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEN8"/>
    <dbReference type="STRING" id="36166.T1GEN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001808-RA">
        <property type="protein sequence ID" value="MESCA001808-PA"/>
        <property type="gene ID" value="MESCA001808"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811016_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026555">
        <property type="entry name" value="NSL3/Tex30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136:SF16">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136">
        <property type="entry name" value="TESTIS DEVELOPMENT PROTEIN PRTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001808-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="15985" checksum="03E87DFE9CAD0EEB" modified="2013-10-16" version="1">MDLDTNLNMVPTLVDKMVFGRPNPTYSKEVLSPVLMDKWEPIITVKNRPLPETTVFVVIPGYPTTGPVPDRIQKWYKLFGTCTQIVQINMAVGGNTLTKQTFDQVAEQIVSLTRLKIKDLKTENSNRHIVLIGFNAGASLALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFB7</accession>
    <name>T1GFB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Immunoglobulin I-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFB7"/>
    <dbReference type="STRING" id="36166.T1GFB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002054-RA">
        <property type="protein sequence ID" value="MESCA002054-PA"/>
        <property type="gene ID" value="MESCA002054"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645110_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin I-set" evidence="1">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002054-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8314" checksum="B18AC4539901ADFB" modified="2013-10-16" version="1">MVKSKYGEVRTANYNVSQAAKLILLEPATLSLMDDVDILPESIEDINGTLTFRNVSSESKGSYTCVASNSQGEITAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGF2</accession>
    <name>T1GGF2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197648">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGF2"/>
    <dbReference type="STRING" id="36166.T1GGF2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002472-RA">
        <property type="protein sequence ID" value="MESCA002472-PA"/>
        <property type="gene ID" value="MESCA002472"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2729632_0_0_1"/>
    <dbReference type="OMA" id="CKHANSF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002472-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8477" checksum="C04BFFF2845F558A" modified="2013-10-16" version="1">MQCKHANSFHQTSKWIDDVRTERGSDVIIMLVGNKTDLSDKRQVSTEEGERKAKELNVMFIETSAKAGYNVKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGG6</accession>
    <name>T1GGG6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein BRICK1-B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRK1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02052300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGG6"/>
    <dbReference type="STRING" id="36166.T1GGG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002486-RA">
        <property type="protein sequence ID" value="MESCA002486-PA"/>
        <property type="gene ID" value="MESCA002486"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175202_0_0_1"/>
    <dbReference type="OMA" id="WEQREFI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008064">
        <property type="term" value="P:regulation of actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000017">
        <property type="entry name" value="BRICK1, SCAR/WAVE actin-nucleating complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033378">
        <property type="entry name" value="BRICK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33668">
        <property type="entry name" value="PROTEIN BRICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33668:SF1">
        <property type="entry name" value="PROTEIN BRICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002486-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8745" checksum="A05CAA146BC454C8" modified="2013-10-16" version="1">MSSVHKEVQKQIHQDWANREYIEVITASIKRITDFLNSFDMSCRSRLAVLNEKLTTLERRIDYLEACVTQGETLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGI6</accession>
    <name>T1GGI6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGI6"/>
    <dbReference type="STRING" id="36166.T1GGI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002506-RA">
        <property type="protein sequence ID" value="MESCA002506-PA"/>
        <property type="gene ID" value="MESCA002506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018084_1_0_1"/>
    <dbReference type="OMA" id="NAYAQMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000009">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="41418" checksum="8D243D3C1FB2F7B7" modified="2013-10-16" version="1">FEAAWALTNIASGTQQQTRIVIEAGAVPVFVSLLSSTAEDVQEQAVWALGNIAGDSPACRDFVLNSGIMMPLLQILSNESRISMTRNAVWTLSNLCRGKNPAADFTKIVKGLPILAKLLHHQDIDILCDTCWAISYLSDGPNEKIQEVINAGVSRRLVELLSHSENNVVTAALRATQVIINCNALPCIKSLLMSPQATIKKEACWTISNIAAGNRQQIQAIIDNDIFPALMGILAISDFKTKKEAAWAITNATSSGTTDQIKYLVEVGCIPPMCDFLTVVDSDIVQVALNALENILKTGEKCNEKPNPFAILIEECGGLDKIEYLQSHENRDIYYKAFLIIENYFGNEEEDHRCAPAWSNDHSEYTLKPPSTGGGFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGV4</accession>
    <name>T1GGV4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02136281">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGV4"/>
    <dbReference type="STRING" id="36166.T1GGV4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002635-RA">
        <property type="protein sequence ID" value="MESCA002635-PA"/>
        <property type="gene ID" value="MESCA002635"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_33_2_1"/>
    <dbReference type="OMA" id="DECFKMK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000025">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP19-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF565">
        <property type="entry name" value="MOCA-CYP, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002635-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="21185" checksum="58AE5B64A2BB9CFC" modified="2013-10-16" version="1">MSKDSRKFPSTLHRRAGTSLTTNKPLHYKGTIFHRVVKDFMIQSGDFSNGNGTGGESVYGGTFEDECFKMKHDKPFLLSMANRGANTNGSQFFITTQPSPHLDNIHVVFGQVVSGHDLVKQVEQLPVDKNSRPLQDAVISNCGELVKVSKSKKEKKKKRKNEASSEDSDSDEEKEKKKKDKKKKKKGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGV9</accession>
    <name>T1GGV9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGV9"/>
    <dbReference type="STRING" id="36166.T1GGV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002640-RA">
        <property type="protein sequence ID" value="MESCA002640-PA"/>
        <property type="gene ID" value="MESCA002640"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_265124_0_0_1"/>
    <dbReference type="OMA" id="HEASMIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="688"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="840"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="885"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1203"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="707"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="946"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002640-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1258" mass="141956" checksum="DEFB465E42D4ADD1" modified="2013-10-16" version="1">MGDDGCDANMNPCMIHIIKESGVDIKKNVRYKSTMGDDGCDATSSDCDATQVEDLPGYSPELKKRHRLNSNSFIYLEGNEKISENSSSNSSEVSENIPVESLNINRGLKEKEFEVCFNDSFIENLAQNINKAKSIHKQTPAKPDEIPTSDAKANQESSSQTLTGNILDELIRMSWKTQTMIQLFMKSSQTPLVTTTSEHELESNSQKENSTNEKIINSQQEENLPPTENETELEPLPLIPTENPMIFLDSSGHQYILMNGNAYPVYQGPPPKDAQITNLQTKPISNNTPQTNKILNLKGRKSFSTPRKHSHVRFLDFSPRNETNSVDKQNTTAPPKIEVQRVEIMNKAVDTPKRSVKLADQVAKPAQKDPEPEQAEDKTVDESVKTVEVDKSVKQEVTTPAPKLEGAKKRKSRAKEKPIYPVPKTPEDLLPKSNEEAMEAWTRLKSVNNSNFDSVLRKTQELIAPNCKKEATNKKKKNRSINKTPLKPIPKPTIKSALKSKEAESKIAEQKSSPENDEKHVHFSEDVPLSNLVQKTPLVTNKEVEKRSANIACLLDTPFKEDDQFVIPPTPGMTVLSQTLDTPLVKVTNLLQSTEKCETHGSKPNTPFAVTPGNYHDTPQSTQVEYSSGSSYYKPDEPEDLDKQMDRQFKEELLRRKKAVAEGMTPRSIEEVVDERVLPANVAPHYVMEEPLPENVEELDKDELSDAESTSSSSSSDSSSSSSSSSSSDSMSVISSEEDKSWEVQKAETEIEKHLPEKPIISNDGEVRYPLRNWITPKKQDPEEVITSIPKSVPEKPPIDEKKLITSLNEKKNRIKEKLSKDFAGGSCQLKIPVKRIVKTKIPTGTKKPQSKIEQVKKPEPQSPEPPVKSAEFLDALRLSAKKVDSPVKEKVVEESAPSAVHDPKTPVRQTKAIDALPLSSRRTLRTRRPTLKAQSPVKIEDSPIKKAKKTVKKAPVKKVQATKKTNSVEKPVSQSKYKASSKRISSSFEIDIEIIKENKADKKGDSSDFKEKEENKSKNVKEKNNEEKEKSLVVEKVSKEKIEERTNEKVQEISKENSEKISKEKTGSTSKEKKESTSKEKIETTSKEKKESISIEKKENTSLEKKENTSKEKIKENILNTKIIEEREEGEWDDDDEEDEEDELEIRSSNEEKIFKFEYKGNSQPTFVRNPRLYASQMNINMEDQKISLTIPKMFHISEQVPLNKAKEPSKKKKVDVEKEKEKERPMECIRPISTSTPYNKPTFKILHKNRNMDESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHU2</accession>
    <name>T1GHU2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Component 3 of promoter of RISC</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Ring-shaped heterooctamer of six TSN and two TSNAX subunits, DNA/RNA binding occurs inside the ring.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the translin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02112675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHU2"/>
    <dbReference type="STRING" id="36166.T1GHU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003003-RA">
        <property type="protein sequence ID" value="MESCA003003-PA"/>
        <property type="gene ID" value="MESCA003003"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079179_0_0_1"/>
    <dbReference type="OMA" id="AHGMEYW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14819">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.190:FF:000001">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.190">
        <property type="entry name" value="Translin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033956">
        <property type="entry name" value="Translin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016068">
        <property type="entry name" value="Translin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003003-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="24051" checksum="3C90BBBBBAF96831" modified="2013-10-16" version="1">MSDNFVNEELFSKFQEYFDHEQELRDNVRQIIRTIETASKKADTKLQVIHSCIDKERIGAVCAEVRKDYFSECVEGFKNLAEIIPTGQYYRYNDHWNFTTQKLIFLIGLTVYLETDGELVTREQAAETLGLKCNQSDGFHLDIEDYLLGILQMASELTRFATNSVTLGDYSRALKISKFLADLNSGFRCLNLKNDSIRKRYDVYKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI07</accession>
    <name>T1GI07_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003074-RA">
        <property type="protein sequence ID" value="MESCA003074-PA"/>
        <property type="gene ID" value="MESCA003074"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113867_0_0_1"/>
    <dbReference type="OMA" id="MCEDEFN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF12">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003074-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="25094" checksum="005827174C222305" modified="2013-10-16" version="1">MPMFRDGFPFFLDMLKTIPDLQEYVPIFERILANSPVDKAYLMFDNCKANWMVLTHGDFHLKNTMIRYNDDESVDDILLIDFQACCWGPVVTDIIYMLYLMLDSKDRLERRDEAVYHYYKEFTDTLHQIGFSGDFPKLSDLYKDILLFKDYELLLAISLLPYVPQAMRGGVEIEVFMNNEQMRRDLYQQADYHDEVRKLLPYFLHRGYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIB7</accession>
    <name>T1GIB7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003187-RA">
        <property type="protein sequence ID" value="MESCA003187-PA"/>
        <property type="gene ID" value="MESCA003187"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796874_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003187-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7562" checksum="44E077BFE565A6F4" modified="2013-10-16" version="1">MAAKDLAAGICEIYQAPLNNIKSELKELISKQNQIYIDIAGEKFGFGNDEVLEVQNMVQKLFSNNVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIN5</accession>
    <name>T1GIN5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003318-RA">
        <property type="protein sequence ID" value="MESCA003318-PA"/>
        <property type="gene ID" value="MESCA003318"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119225_0_0_1"/>
    <dbReference type="OMA" id="QYAKANC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052295">
        <property type="entry name" value="Odorant-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066:SF9">
        <property type="entry name" value="ODORANT-BINDING PROTEIN 59A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066">
        <property type="entry name" value="ODORANT-BINDING PROTEIN 59A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577404" evidence="5">
        <location>
            <begin position="21"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003318-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="25304" checksum="8A504A647237083D" modified="2013-10-16" version="1">MKSLLGAFVILLVVGVSVNSLQCGTNGLPVSQDMRRIYKTCMQQSTEPNYNNRHYQQQGGNGNSNGNNNMNTNNNRRGDYNDYNDDFYYRPRSGGRFRRQMTSSDSSNYRQSSSQSSSTSTAAKSGDCMTHCLFKELNMLNNEDYPDKHKLYYLLTRNIRNTDIREFFSDSIHECFQFMEQEKRRDNACDYSKNLLNCMIQYAKANCDDWDDHSLMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ54</accession>
    <name>T1GJ54_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003496-RA">
        <property type="protein sequence ID" value="MESCA003496-PA"/>
        <property type="gene ID" value="MESCA003496"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485904_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003496-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10276" checksum="E4A8BB97EEF3DAFC" modified="2013-10-16" version="1">MKKQFRTTTLIYSEEIEKNSLSKTLRWTTIGVENVTENKKLFIICLEDVIILDNGKTALYWNRTVINDLTIQHKRPDILMHNKLNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJK9</accession>
    <name>T1GJK9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Odorant receptor coreceptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family. Orco subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02162314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02162315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJK9"/>
    <dbReference type="SMR" id="T1GJK9"/>
    <dbReference type="STRING" id="36166.T1GJK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003658-RA">
        <property type="protein sequence ID" value="MESCA003658-PA"/>
        <property type="gene ID" value="MESCA003658"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_288417_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026805">
        <property type="entry name" value="GW182_M_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF9">
        <property type="entry name" value="ODORANT RECEPTOR CORECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12938">
        <property type="entry name" value="M_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0085">Behavior</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="GW182 middle" evidence="16">
        <location>
            <begin position="897"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="488"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="666"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="488"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="666"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="742"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="789"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="801"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1066" mass="118602" checksum="AD82935B453D92E1" modified="2013-10-16" version="1">MYSTIHLILVFFQFSSMLINLALNTSEVNELTANTITVLFFVHAITKFIYFALNSKSFYRTLNIWNTSNSHPMFAESNARYHRIALSKMRKLLIFGGLATLLATISWITITFYDESVALSMDKTTNETFKIPVPRLPIKGFYPGNPFSGTYYYAIFGFQVYYLVFSMLHSNLLDILFCSWLIYACEQLQHLKYIMKALMELSASLDTFRPNSAALFRNFSGENNKDKPIENTDADITEESVYNTKSDWNAEFRAPSTLQNFNNNINPNGLSRKQEMMVRSAIKYWVERHKHVVRLVGSIGDTYGTALLFHMLTSTINLTLLAYQATKITGLDAYALSVAGYLIYALSQVFHFCIFGNRLIEESSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQKAMSISGAKFFTVSLDLFASVLGAVVTYFMPGQNTNAGNGANNNTTPASSGSSNAPSSTGTKNQLEQLNTMREALFSQDGWGYQHVNQDTNWEVSVSPEPQQKDNQPLSNTSSTGATHNLRNGGQPPVQIPVQKTPWGHTPSTNLGGTWGEDDDGAESSNVWARGPVESSSVGVGNVSNQWGQQHNANSTAPATNTWTDVREIVQRQNTLELHRPSTNPTNTVTDLQPRNVTASGDPIRAILEQREPIRGDPRGISGRLNGTPEMWGQIPQLQQHQTQNQLKVQNTGQWTTAPEKPTVKPPTIGSGWEEPSPPPQRRNIPNYDDGTSLWSQQNRWKQQEENRSSHILRANANSSNTNNTTNRSQKNDNSGMWGGRSNNSAGNNNGGGNQWETDNDNSTNNTTTNSDWNKPSKLTSNWDEPSSSAVASSNAAVGSNWELKNANIKFMDSSNMNFDEALEYLSNNWRRPEPEPTVNYDRSYGRVVGSSVPPQFPTTHVQQKYSGFGQQPNAIVATALNNVGGGNSTSSHSQEPTAQQLRMLVQQIQMAVQSGYLSSQILNQPLAPATLVLLNQLLTSIKHLQGAQSSVQRGNNMNSMQISVTIAKYKQQITSLQSQINAQQAIYIKQQQIQQQQQNQHVGSNEFIRNHHEAISALQGNFNELNLSKLQQINNPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK68</accession>
    <name>T1GK68_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02011032">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK68"/>
    <dbReference type="STRING" id="36166.T1GK68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003886-RA">
        <property type="protein sequence ID" value="MESCA003886-PA"/>
        <property type="gene ID" value="MESCA003886"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323044_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19991">
        <property type="entry name" value="L 2 01289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19991:SF3">
        <property type="entry name" value="LETHAL (2) 01289, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003886-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11557" checksum="4F142CFAFA331519" modified="2013-10-16" version="1">MLDKLLEENEFITVFFYEQKQHESTAALEKLENIDSETDNLDITFVKMADARYARNGESQNFQLWYTLEEGSPAFTEATFSLRMKSWNGCERTDSDNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKF7</accession>
    <name>T1GKF7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150582">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="MESCA003980-PA"/>
        <property type="gene ID" value="MESCA003980"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173846_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003980-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="13007" checksum="C4646229167C00D8" modified="2013-10-16" version="1">MPICVSTKILINMEKARKSAQKTFQNYNWFPLRAEENKRKKRAERQRQQQRQRRGAKVFHSINESTGHLTLAPSRLFEKTPMSPSDSLDEIALQIPRNKIQYKHKENSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLD4</accession>
    <name>T1GLD4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058672">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058673">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004334-RA">
        <property type="protein sequence ID" value="MESCA004334-PA"/>
        <property type="gene ID" value="MESCA004334"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925232_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004334-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6863" checksum="038BF869FE412FD0" modified="2013-10-16" version="1">MCLANTSLRYDLETKSTDFGYVGGFAPAVGDVNMKYANGVNIVRFLHVQIKFMSWGEHHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLJ4</accession>
    <name>T1GLJ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155008">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004399-RA">
        <property type="protein sequence ID" value="MESCA004399-PA"/>
        <property type="gene ID" value="MESCA004399"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514288_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004399-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="21008" checksum="86E704BAB3701160" modified="2013-10-16" version="1">MQEVQETISWFNLEFQQAIEVFHGIDINTFSNVEDRTIWINDQSLKAVSKIIEKLNEIQTSESILCVQQRKLFKILSIREKYNIFYNLIGNLLQNYQGFDKTYLYKVKENMHQNSLVLFNHPRRVLRTDLTFKSSVIWLLDGHLRNPNTVTVYLLTEKAAQEFSNDNRNLKPSGVIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLP0</accession>
    <name>T1GLP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLP0"/>
    <dbReference type="STRING" id="36166.T1GLP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004453-RA">
        <property type="protein sequence ID" value="MESCA004453-PA"/>
        <property type="gene ID" value="MESCA004453"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852267_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588383" description="VM domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7200" checksum="5FF471FC3EDA69FE" modified="2013-10-16" version="1">MEIRHIFHTFVITLVLLIKTTKPHNSCGLYGEPPCIFVPAPPGQTPHCARPGTTFCEHVANYPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GML4</accession>
    <name>T1GML4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02088748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GML4"/>
    <dbReference type="STRING" id="36166.T1GML4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004790-RA">
        <property type="protein sequence ID" value="MESCA004790-PA"/>
        <property type="gene ID" value="MESCA004790"/>
    </dbReference>
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    <dbReference type="OMA" id="XKRIERR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004790-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13647" checksum="D31404E66EF90EA5" modified="2013-10-16" version="1">MKQTRLSQALCADPFSYVWKPLLIGGYAGRKSIIVKTYDDGTSDKQFGQALVVSIDRYPRKVTKSMSNTKLKKKFKIKPFLKALNYNHLMPTRYTVSDISLDTPFPNQSQVNANDTKDHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMN8</accession>
    <name>T1GMN8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMN8"/>
    <dbReference type="STRING" id="36166.T1GMN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004816-RA">
        <property type="protein sequence ID" value="MESCA004816-PA"/>
        <property type="gene ID" value="MESCA004816"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778769_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004816-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7682" checksum="FD7D5225CBF821B5" modified="2013-10-16" version="1">MSPLYATPPHSPTSELGSPMQLPLNGFDDYRSHNTSGTSSNSKPDSHFARDTHMRIILGNLKEQNQSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMZ6</accession>
    <name>T1GMZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA004934-RA">
        <property type="protein sequence ID" value="MESCA004934-PA"/>
        <property type="gene ID" value="MESCA004934"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1416639_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588412" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004934-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21828" checksum="0D86CE01B671D25D" modified="2013-10-16" version="1">MLNFGRVFKFFLIVLESRSLVCRASALHTEFQXPNYYSNVNNWWDGFDSQISCPIRDSKDSVEQYYLLFIWNDFHSCCRGIVATTSNYNVLLFAFFLIGFGKGFRTIFSPLIIPSYVPLNRLPAASGLQLISTGIFTISCGPLVGLIRDAYGFSVTIHIINALTYISIFGWGIMSFVMRRNRQKKTADTAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN69</accession>
    <name>T1GN69_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN69"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005012-RA">
        <property type="protein sequence ID" value="MESCA005012-PA"/>
        <property type="gene ID" value="MESCA005012"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640939_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8420" checksum="604527199FCE6BD8" modified="2013-10-16" version="1">MFCCPESSVIFKIFGLVLRKEVFTYEPISDDHQCTGDPYNICGGPSRKQPLCGAILATDGFIVGLQAGVVNFFKCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNL0</accession>
    <name>T1GNL0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005167-RA">
        <property type="protein sequence ID" value="MESCA005167-PA"/>
        <property type="gene ID" value="MESCA005167"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624833_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005167-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8926" checksum="8324AC1C44ADAA20" modified="2013-10-16" version="1">MKCTASVGFLFPLNLKLHLRTTSINSASSDFLRQQESCRQDFPRLEEVVLGLKGFGVWRGPYSFHGMFSSFHNWAVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP86</accession>
    <name>T1GP86_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP86"/>
    <dbReference type="STRING" id="36166.T1GP86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005407-RA">
        <property type="protein sequence ID" value="MESCA005407-PA"/>
        <property type="gene ID" value="MESCA005407"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1541858_0_0_1"/>
    <dbReference type="OMA" id="WELAVCI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="20194" checksum="F3411DB9F70DA7AF" modified="2013-10-16" version="1">MWSFNKNFFKSAKNLNEIKIKEYLQNHPEYLDNYVLSYVEPDRIQTWLERKKNDKNRDYCAVAVPKNCPHSEGHQMIQDLSQTLQHQPTKYDVLVELGNCISSAINADGFTLFLADEGDDSLYTYMGQNARGEAVLQLAKTQEVEVPLYVSKQRKAIRLSKNNGDYSNYFKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPB1</accession>
    <name>T1GPB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02005023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPB1"/>
    <dbReference type="STRING" id="36166.T1GPB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005436-RA">
        <property type="protein sequence ID" value="MESCA005436-PA"/>
        <property type="gene ID" value="MESCA005436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_40_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21780">
        <property type="entry name" value="MobB_Trc-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF184">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TRICORNERED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="97"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ71</accession>
    <name>T1GQ71_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA005772"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005772-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="12066" checksum="445C0B7578596C76" modified="2013-10-16" version="1">MVKARKLKFGTECTKARVRQCPSLVLVEVYKDSSKEREEAKASLVEEDIDSKVKEVRQILNSAAQEKDVTWEKYKNGKRDLCYIIGWAKKDSWVSFLRRHQKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQE9</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
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        <lineage>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <scope>IDENTIFICATION</scope>
    </reference>
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            <dbReference type="EnsemblMetazoa" id="MESCA005853-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7652" checksum="0FD3D1FED66A0FA4" modified="2013-10-16" version="1">MNSYYPPILKRQNCLRYIKEMITSLWKPLDYQTSGLRMEEHSCTIRSPALPILALKSFVITEYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQJ4</accession>
    <name>T1GQJ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblMetazoa" id="MESCA005903-RA">
        <property type="protein sequence ID" value="MESCA005903-PA"/>
        <property type="gene ID" value="MESCA005903"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005903-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="54" mass="6451" checksum="E40DAA798A7CE00B" modified="2013-10-16" version="1">MSMLYERMGSKHRKDSITLFACLSLYDTLQHINVPKDEEFIQNDQRNHLTKPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQJ5</accession>
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    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="term" value="P:epithelium development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RhoU, isoform B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RHO FAMILY GTPASE"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005904-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="334" mass="37488" checksum="0C102D3566AB751F" modified="2013-10-16" version="1">MGYPYGSPPSPRSPVFETQKNDFLFSDKTNYQTNGGNNYYNSNVNNWQQQQLQQQFQNNHHLHNPHHEPQDIYDSVINNSKYSGLQTSLSKSDTSSSSRSSNGNGSKESNFLVKTKKFTKSKAEKKKSKKKQKEHEKSSSKLPNIKCVVVGDACVGKTNLIVSYLDNRFIPEHNPTASDIYNAEVMVNDSPVNLNLCDTAGQDTLDPLRELCYPDSDVFVLCFSTVKPETFQSIKTKWAPKFSKTKASLILVGTQADLKNDIHVLNKLQMNGEKPVSGSDAWDFAASIGAQYIETSSYTQEKVKDVFDAAIWDALLPKTLPPKPPLWKRMFCLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQL0</accession>
    <name>T1GQL0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo repeat-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQL0"/>
    <dbReference type="STRING" id="36166.T1GQL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005919-RA">
        <property type="protein sequence ID" value="MESCA005919-PA"/>
        <property type="gene ID" value="MESCA005919"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1497929_0_0_1"/>
    <dbReference type="OMA" id="PKWFLER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 4 ARMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005919-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20239" checksum="7EF017BA91839D5C" modified="2013-10-16" version="1">MAHTGFTMKYKKDTYNPLCGNCPEQKKLDQEYDSDSTEVMSSTDEEDRWKDAARTAEIPATYYHIQKLVRYIKSGNQTATIVSLCCLLDYDLTIQINQLAIQDIGGLETLISANIDIRKFIVDLGGVPLITNILDTSNKALKTMAAQTLAHVARVRLARKFVRKCKGIPKLVDLLDIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRA9</accession>
    <name>T1GRA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006189-RA">
        <property type="protein sequence ID" value="MESCA006189-PA"/>
        <property type="gene ID" value="MESCA006189"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690646_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006189-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8699" checksum="32DE36D48C64BF20" modified="2013-10-16" version="1">MDDNAASVNKKSARQKHFGLYESSWWNLQQFQDYKRFSTYNYFNIPLAMIMRVTNSSISNMLKGNNQSDPGRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRS5</accession>
    <name>T1GRS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRS5"/>
    <dbReference type="STRING" id="36166.T1GRS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006365-RA">
        <property type="protein sequence ID" value="MESCA006365-PA"/>
        <property type="gene ID" value="MESCA006365"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_732139_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00072">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="1">
        <location>
            <begin position="312"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006365-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="378" mass="43390" checksum="21B693D21D5939CF" modified="2013-10-16" version="1">MTDPIKFGPGWLRNQMSSVDEFRSPTLSVIGANSCSLSANNGGVAKYRYGREEVIATFDKDLKPPEIFYKKLFVDKIQPPMALHPVDDENVSVKNWGGRISNYRSSRAPRGFSYGGFNRGSENDCQSQFNESWKNQNFSLDSTIKFPSTDVLNILPADTTTPEMSSTMLEITPLSNLINPNTRFFNNIDKSCLNNQLPSPNNSWNSTISPLRKEFNNRNTENWRENWRGNRQNWINRSNSWRETEPETEKINESLPEWAIDTPVNSGGTFDSSGAFLGCQESVQDPATEEVVDMVEKLIMEESEPEQKEDIGRKWFYRDPNLKVQGPFTGCEMTEWYNHGYFDENLYVRREIDGRFATLGEFIKLCGNIRFISIKLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRT5</accession>
    <name>T1GRT5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006376-RA">
        <property type="protein sequence ID" value="MESCA006376-PA"/>
        <property type="gene ID" value="MESCA006376"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944338_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6722" checksum="F8F1F336B0BE273B" modified="2013-10-16" version="1">MFCSPKTSASKRNRSQHDIIRSPEVDLTAPVIPIPMECWTLSRVLQSPLTGSAFHQTRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSI7</accession>
    <name>T1GSI7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006647-RA">
        <property type="protein sequence ID" value="MESCA006647-PA"/>
        <property type="gene ID" value="MESCA006647"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944353_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006647-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="7034" checksum="3D1A409B9CB50810" modified="2013-10-16" version="1">MERGRDMRSIPLAYPQHPVVFHNQMYLCTYIKVEVWRTSTVSKLISSPLLDSIPLHCFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT01</accession>
    <name>T1GT01_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02055133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT01"/>
    <dbReference type="STRING" id="36166.T1GT01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006817-RA">
        <property type="protein sequence ID" value="MESCA006817-PA"/>
        <property type="gene ID" value="MESCA006817"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_532404_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF13">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="179"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="321"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006817-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="512" mass="59161" checksum="3E663945AD71CDA8" modified="2013-10-16" version="1">MCRFHLANLDAEEIYEEFLSSNKTSHDKLLNILKILSKNHPNQSEILQEAFIVLQKQTPQQCLLKFLRRMDSYNVYSKKEISTPMMSSTKAYDFSTPEILENLKEKIHAVASTSICRSQLNSSISNQLNTSIPLTPISSPSLYSLNNNSHFKFNLDLKEYDNEINIISVPILSQEMILLNEILYSLLGIRGNYIQQIEQKLPSGNTEINFKVSDQIQKSLRDVANEILPLGCYYYYINNFTENVRFFNCGQVLESFTASLKDLLHDYKVSIISQLEFQYISGKLTIHKLHFYLRPTINTMETIFNIVNDISTNLEGKPESQKFLPNTANLYRGAFTLSQRMMNAIQNLEYYMMIEVIEPFWHDFIAKIKDKIENVDAVLIAHSNFLDNCLRNCFISDPVLLFGISQLCQVCLKFCEFIQKSQRLFLDAELKSMLCDSFDDISDCDMDISVKQDESSIKSSDTFSEQVKKFNTDFSSLLIGLLKQINDAASENPNDRYLNLVHRINFNNFYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTB0</accession>
    <name>T1GTB0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTB0"/>
    <dbReference type="STRING" id="36166.T1GTB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006936-RA">
        <property type="protein sequence ID" value="MESCA006936-PA"/>
        <property type="gene ID" value="MESCA006936"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046597_1_1_1"/>
    <dbReference type="OMA" id="ENHLVMT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF38">
        <property type="entry name" value="HL01515P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="60"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006936-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="299" mass="34327" checksum="B219A9E5172CD30A" modified="2013-10-16" version="1">MTPQIETGVVKFDDNQAGYIDLGTGKIRLEKEEPAEWAVEIARKELRETPENVEKAIAELKELLQQQKHLYVPLDDDYMKMFLRPCHFYPEGALKRLVNFYHMKLKYKDSCEDILPASLKHVFAEGILTLFPKRDQHGRRILCLEAGKKWKPSKVPLNDIFKGIQLTVMGAMAEPTSQICGAVVIIDMEGLPLSHITQFTPGFAIMLLDYVQECICLRLKGVHVPFIKEKLRKRIFFHGKDFNSLAKHIDRSCLPAKYGGNMECELPPGPALGDFFDCYRKDFEMANSFGFTKDYVYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTS8</accession>
    <name>T1GTS8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007119-RA">
        <property type="protein sequence ID" value="MESCA007119-PA"/>
        <property type="gene ID" value="MESCA007119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796865_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7773" checksum="AF15DB5A1AB2E25C" modified="2013-10-16" version="1">MVKVKQHLLTSEVKDNLVAVSFSPQLAPGKEIEHLLEEYFQNIALNSKGKNLMGKRARFQLFFPLLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTV3</accession>
    <name>T1GTV3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTV3"/>
    <dbReference type="STRING" id="36166.T1GTV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007147-RA">
        <property type="protein sequence ID" value="MESCA007147-PA"/>
        <property type="gene ID" value="MESCA007147"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870175_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="849"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007853-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="877" mass="98365" checksum="595ABD7CEDB55AAC" modified="2013-10-16" version="1">MLKHLDIQISGFDKILPPEEKIITDLKSSISENVKTKYRAAAAVGKAITTTSNSSTMKPTEDTSSASKASDVHKDKDAKEKDKEPSLNSAVETNDGKIKQPQQKKKTKANKPVIKKVKTITASAILVKKHKSVKTVKKLVVPHKNNKDVEKPQKKEVTKKDKEEEKVKEKEKEKDAGETGKDKAPKKVATRKVLPSPVTSVKQQNVATKRSRSAIADMLTTPSPVAKKVSSQKRTPTPRSKSLEVSKKSKICEEKNSEDPPAKEEPPDKKDKLPPEKDIPIVEETVPPKIVEEEKQIEKIAEEKIIKAETKDNKEIEKKPQKDKDIIQKEDKKDCNEKMENIVVLEVSKDVEKKETAPPEGPIKKKPIKKKEPSIPKATLRKYNLVPKQLLKKLHKKVLPKPIEKTKKPLEDEPPKKKDVYDFKSGSESDEEIKMELPTLKILKEYSDEDDKPLGLLTKDKPTVSENPSEVIESPKVEPQKVDKVEEKEKPKPPKKDKKSDEKSTVSPKKETKPPAKKPPKKEKIIISSSEIESSSSEEEIYLGPKRHRMASLNASAKVQCLYENESRTAQELGLSRTIVHHLPKVQTIPGAEAETAKVIEKPIVPVKKESSDEKDSSDVEIADMKRELRNAPGLRGAGKLGNSMTCPVLRVPMTLMKKAQKNEKDALAKKAVEKPKKILEKKVKLVKPKEAKIDPRKRLPLKKRKRPKKEDSSNESGVILGKKRIASLNASAIVAATYEVERHLDRNLATDCSSYESLVEEVKKDSIKNQDNLHQQNSPKKIKQEKDIKTEIKEELKDVSRPTSTSVVIVQDTDVTITGMYVNTATGSSQESYCKMQYRVTEERVVRPSSSEPPKSYTPLSALSNSAHPWKQLEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVQ6</accession>
    <name>T1GVQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394928">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007872-RA">
        <property type="protein sequence ID" value="MESCA007872-PA"/>
        <property type="gene ID" value="MESCA007872"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212934_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007872-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12155" checksum="7E886748CB566DA9" modified="2013-10-16" version="1">MVQEVKIAAQDIEAVFGVYKDRMKSQLQLAGAISNSSFYLERNLEKRKYVKTVETELSLRTSDQLACLIINAISTDMNSEEHTPTVGASDEFKDIFSEQLSKTYSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWH3</accession>
    <name>T1GWH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras-related protein Rab-30</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWH3"/>
    <dbReference type="STRING" id="36166.T1GWH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008150-RA">
        <property type="protein sequence ID" value="MESCA008150-PA"/>
        <property type="gene ID" value="MESCA008150"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_24_2_1"/>
    <dbReference type="OMA" id="QELNTHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008150-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="22299" checksum="DBE0240D36ED536D" modified="2013-10-16" version="1">MEDYKFLFKVVLVGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEVEGEKIKLQIWDTAGQERFRSITQSYYRSAHALILVYDISCQPTFDCLPDWLREIQEYASTKVLKILVGNKTDRDDREIPTQMGKNLPNNTTCISWKHQPKKPKMWKGYSMKLPRNSEIKPEARTPRFQINNEITPVIQLDCLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-01-19" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWN0</accession>
    <name>T1GWN0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008208-RA">
        <property type="protein sequence ID" value="MESCA008208-PA"/>
        <property type="gene ID" value="MESCA008208"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294840_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008208-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11836" checksum="F28816B70FAF0081" modified="2013-10-16" version="1">MDDYDEYVVQVQPNTMPVFEGYLQTNMPSIAVYGTGRLQIHSNGNGFFISDRPRSDTLSQLWRRYVYDRKLYALPLTDLHGRFRECTAAYYRSCVNRSNXI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXV5</accession>
    <name>T1GXV5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXV5"/>
    <dbReference type="STRING" id="36166.T1GXV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008665-RA">
        <property type="protein sequence ID" value="MESCA008665-PA"/>
        <property type="gene ID" value="MESCA008665"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_347260_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="639"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008665-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="813" mass="90927" checksum="E9CAF398F5560C89" modified="2013-10-16" version="1">MSSSLQSDTEFSEDNAYDVAKKNSKCLKDICRNETNLSLLSSKMNSMFGKELPSTGRGRKRKQKTPLPNPKDGMEEDDAYDPMEQVRRKQNAQLSLVESFLTTTAQRLSQHPNVLEDVAGKMHAAAVANNELQTDDDNNFHRQMPSNQKDVASNPMSTCQNLVVSKGSKKWISGDDDRMPLQRECGSVDTIQADPSKLIPNRDFIARFMMNNAGGDGHHHHHHHPNPCAHEEDENSSFLDVVDSNNPTAGVASQLPQYHHNTPNEDDDPGVVHGTSQSFMNSFYNNSNPRLTAPGANNSSASLLVEAALNSVGNMIENVTMKLRFGGNGLNSIDSLENEIKMMKGLNNFPMNIPTLPIFPNSNNTMSSCNMSPATPNQRTPQNDIDVDANSTPRRAQLSAGGESERYSNHHHHTPPSPAVISPGRDYSMFNNSANGPGSAPNNLSVASPMQVRRGPTSSGTVYPDNELISPASSPAIPRYNFNNELNRKRILEDERSQNMSVNNQMSSDDENSIIQQNSTPTQDMRMKFAQSQMDLMYSKYESMASAANLKYNNHHQDLEVTDYRNNTPSNDMSDMQGLDMSSRSNNMVQTIIITSNYQHQPQQQQQQQQQTQPQQHTQQQEHTNQLPMQQHQGMHNMMSEQMSDQEHDQNSSVDLSRTANYHHSSPPPLPYSHAHHDMLRMVSLDLTPNSNMTHSNNRSFLSSQMQHNRGEGLDHHRLLQTAEQHRLLAASNPEQHRLLVDPAAHLLMEQNNRLLGSAESSHNNRHMARGFGAYHQVASGNYHHSVRPGVLPSSNHHTPNHTSNYHPFPAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY80</accession>
    <name>T1GY80_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY80"/>
    <dbReference type="STRING" id="36166.T1GY80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008802-RA">
        <property type="protein sequence ID" value="MESCA008802-PA"/>
        <property type="gene ID" value="MESCA008802"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057773_0_0_1"/>
    <dbReference type="OMA" id="WMRSQFV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008802-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25637" checksum="1495B384FCD45058" modified="2013-10-16" version="1">MTMSSNNCESMTSYFTNSYMGSDMHGHYPNGITDLDNQQMHHYSQNPNNQGNMPYPRFPPYDRMAYYNGQGMDHQGYSRPDSPSSQNGMGPQQPPPQLANQPPNGTQLGPQSQPQVQPQAQQPVVFASCKLQAAVGGGLGMPQEGGSPPLEQIGHHINSQMPLPHHMGHPQTQMMHQGQQMHQGQQQQPPNNNQSSQNMNSPLYPWMRSQFGESRELPFILPMSRLEVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYB9</accession>
    <name>T1GYB9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008843-RA">
        <property type="protein sequence ID" value="MESCA008843-PA"/>
        <property type="gene ID" value="MESCA008843"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2229844_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008843-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12394" checksum="60E2456B78469039" modified="2013-10-16" version="1">MMLWEHNFPYIRCHGVMLRRSISYMGDNRSLTPNIGRRCNNNQIKIVQKQRLQPPCFVVKYITYRVAADMSKEIIGNLFVVELELLEKCYRGMNDNDMSSFESGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYM3</accession>
    <name>T1GYM3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02102469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008957-RA">
        <property type="protein sequence ID" value="MESCA008957-PA"/>
        <property type="gene ID" value="MESCA008957"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944340_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008957-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6741" checksum="FE65D73F545D7AC3" modified="2013-10-16" version="1">MNVKSKFPLFRVYILSYFPLYTINTRTGANSLLLAAANNQGPKQGNGKELDPETNVPWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYQ5</accession>
    <name>T1GYQ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02375986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYQ5"/>
    <dbReference type="STRING" id="36166.T1GYQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008992-RA">
        <property type="protein sequence ID" value="MESCA008992-PA"/>
        <property type="gene ID" value="MESCA008992"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113164_0_0_1"/>
    <dbReference type="OMA" id="AYDCENA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF51">
        <property type="entry name" value="LD01482P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008992-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="212" mass="24955" checksum="2CDD012513BCE243" modified="2013-10-16" version="1">MLTSRIERECTRKQKCIQQSLGNRNQSNIPHPCSQGNTILSRTAEKVTFIVMLVLSNILPSIFTFIRPFLKWFLHRFTRLCELQRICYGCTKGASRNKHLEKSLALSKTPSIKNMIREFDEYVEHCSDEEFGLLPARGIAAIMYAKKIKPKIHPDFPKLLGKSIEQIWGYRRLMHVVERLREEQYDAEDQRHEEKLLELWKLLMPEVPLESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYX8</accession>
    <name>T1GYX8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137750">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYX8"/>
    <dbReference type="STRING" id="36166.T1GYX8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009066-RA">
        <property type="protein sequence ID" value="MESCA009066-PA"/>
        <property type="gene ID" value="MESCA009066"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1099564_0_0_1"/>
    <dbReference type="OMA" id="NITCMAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000094">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF575">
        <property type="entry name" value="LOCOMOTION-RELATED PROTEIN HIKARU GENKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="19"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="178"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009066-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="27495" checksum="19704F84B26C0C24" modified="2013-10-16" version="1">MPSCVPTTLLTNFSDDSPPSVRIKIGSGSGAFEPSGVLAVLPGSILHLDCMYPRARGSPEWSWTSWHKKYSTAWSSNPEEKSTKYRLTIKDITPQDSGSFTCSSPRGLTNSISIVVASSTCPKLPEPVPPLSLRLEGYKLGNRALYRCPLGFTVEGSINSTCLASGNWSSPPPTCQAVQCPALSLEDSHLSLTELNTSAWGKAVFKCQWGFKLNGPSRFVEVSEKSGPPKRPECLPDGNWSIPIPQCRNYEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ81</accession>
    <name>T1GZ81_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEX28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02100068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009179-RA">
        <property type="protein sequence ID" value="MESCA009179-PA"/>
        <property type="gene ID" value="MESCA009179"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032402_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019394">
        <property type="entry name" value="TEX28/TMCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613">
        <property type="entry name" value="CEREBRAL PROTEIN-11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613:SF14">
        <property type="entry name" value="DEMENTIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10267">
        <property type="entry name" value="Tmemb_cc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="62"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13515" checksum="1A375BBC80CA79F8" modified="2013-10-16" version="1">MQGEIGNISSSNNTALQTATPGNTGTGSGKFNSDGSEMSSVTSESIPAGSGKSGASQYHIVIKSFLAELHERKEESDKLKERIDRLETQQKEYHDLTVLLESERYRSERLEEQINDLTELHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZB9</accession>
    <name>T1GZB9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02134866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZB9"/>
    <dbReference type="STRING" id="36166.T1GZB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009218-RA">
        <property type="protein sequence ID" value="MESCA009218-PA"/>
        <property type="gene ID" value="MESCA009218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134183_0_0_1"/>
    <dbReference type="OMA" id="YANRVNN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF109">
        <property type="entry name" value="FI07618P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50960">
        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="5">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="3">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19053" checksum="BF7093A8E3FE89F6" modified="2013-10-16" version="1">AKLLESSNPWLSGGAGAPGAPPPSSSVAESYQYQLQSMWQKCWNTNQQNLIHQLRFRERGPLKSWRPEAMAEAIFSVLREGLSLSQAARKYDIPYPTFVLYANRVHNMLGPSLDGGVDPRPKARGRPQRILLGMWPEELIKSVIKAVVFRDYRELKDEGGQHLLPNGSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZI8</accession>
    <name>T1GZI8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381808">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZI8"/>
    <dbReference type="STRING" id="36166.T1GZI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009289-RA">
        <property type="protein sequence ID" value="MESCA009289-PA"/>
        <property type="gene ID" value="MESCA009289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1322245_0_0_1"/>
    <dbReference type="OMA" id="TIQGSCA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF4">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="22829" checksum="C5345E81107AC725" modified="2013-10-16" version="1">MTEEDLEKEKPFLGVPISTKDCIAVKDMKHTSGLYARRDIRSKVDSDAMALMRAAGAIPFALTNVSELCMWWESFNTVHGRTRNPYDTNRIVGGSSGGEGTIQGSCASPFGLGSDIGGSIRMPAFFNGIFGHKPSMNIVSNKGQFPIPFCDEQNSFLGIGPMCRHAVDLKPVLKIIAGDKANLLRLDEPVNEDDILIFIKWVMAVAYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZJ0</accession>
    <name>T1GZJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GZJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009291-RA">
        <property type="protein sequence ID" value="MESCA009291-PA"/>
        <property type="gene ID" value="MESCA009291"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850172_0_0_1"/>
    <dbReference type="OMA" id="CARYYLN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009291-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16186" checksum="A18FEC897B3ABF65" modified="2013-10-16" version="1">KKQTLKSFSDIEQLFKQTKKREVKLLLRENNWPINHPIRAQLWPKLCAQHKVGKDMAEGFYWDMVNQDFGTTELPEKPMTLPPFVDAFHCMPYHLTKKGRAVADRIVNVLGYACPDITYSPLLYXXXRQNCQCLGLRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZQ8</accession>
    <name>T1GZQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MGAT4 conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02103327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZQ8"/>
    <dbReference type="STRING" id="36166.T1GZQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009362-RA">
        <property type="protein sequence ID" value="MESCA009362-PA"/>
        <property type="gene ID" value="MESCA009362"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344796_0_0_1"/>
    <dbReference type="OMA" id="SMEERND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF9">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE A, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 conserved region" evidence="2">
        <location>
            <begin position="117"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009362-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23395" checksum="72C163820E5D1C81" modified="2013-10-16" version="1">MKCKIYFLGFLLLIPFTIILIIFGPSNLYTEQSLIQKLTEYQMKIQYLESLYRARQEDLSILTHLLGTIQSTNGNQFSNMSSSFSENNTFYLTLSSETRAALKNISSNIKNSEKLRLPNAYNFLPHLLDEPSSLKPLVHVSAGRTGVTTVLGVPTVKRDKQSYLSGTLTSLIDNMSMEERNDSLIIVFIGDTDSFYINSITKDIETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZU1</accession>
    <name>T1GZU1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Accumulation-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZU1"/>
    <dbReference type="STRING" id="36166.T1GZU1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009398-RA">
        <property type="protein sequence ID" value="MESCA009398-PA"/>
        <property type="gene ID" value="MESCA009398"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001074_17_4_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068">
        <property type="entry name" value="BONE MARROW PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068:SF14">
        <property type="entry name" value="CELL WALL ADHESIN EAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009398-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="12628" checksum="B4CE33F1A091792D" modified="2025-10-08" version="2">MPPFSPLSPFSPCGPVAPGRPTGPLGPGFPGVPDSPGGPGGPGGPFAPSSPLEPCTPGLPGGPLAPGSPGPGTPGLPGLPGVPGNPTFPGSPLVPGAPVFPLSPTDPGGPMGPDIPLDPGYPFGPGRPIVPGLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H019</accession>
    <name>T1H019_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H019"/>
    <dbReference type="STRING" id="36166.T1H019"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009483-RA">
        <property type="protein sequence ID" value="MESCA009483-PA"/>
        <property type="gene ID" value="MESCA009483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534303_0_0_1"/>
    <dbReference type="OMA" id="EDAFQIE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20124" checksum="79386D9CFEED2291" modified="2013-10-16" version="1">MFMEPGEVKTYLKLCKTLDRTEDLPLFPVSDRHLSVVKERVDLARDVDKLELKNKKSSSEIGWMKKTAEEMDMIIDGFGEDGSEISDDEDAFQIERNRNLNQLNRKRIELATILQKPLFPRGFSFKYPTASGALDIPKMKFSEGQSGENDNTAVKVLKKSIEELKLAKKQRNKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0F7</accession>
    <name>T1H0F7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol N-acetylglucosaminyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0F7"/>
    <dbReference type="STRING" id="36166.T1H0F7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009633-RA">
        <property type="protein sequence ID" value="MESCA009633-PA"/>
        <property type="gene ID" value="MESCA009633"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024002_2_0_1"/>
    <dbReference type="OMA" id="WITMQAS"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016104">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30516" checksum="C55D6DC9A5266558" modified="2013-10-16" version="1">MPPKSKKFIESTKPKWEKNLYSNQGYPDNYTDESFLKDLKTNINLKHYTYLEAIQGATFLSNQISCVTCFLFNFYFMNSGELSPSKALLFNGTVTLVCYIAYKIRNLSINDLMESSKTLLTVLVFGYIFSPLLHTLTDAISTDTIYSMTFFVMFLNIAFHDYGLESAFTVFLVPCICSFGIAAVYFVLYPIFSRRFWKSYLLIPLSGGVCYYLSEISVPLLYTYVAVVVFINILCPILFVRLQRHKNNIYGPWDEAVVDIGDKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0L4</accession>
    <name>T1H0L4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0L4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009695-RA">
        <property type="protein sequence ID" value="MESCA009695-PA"/>
        <property type="gene ID" value="MESCA009695"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044267_0_0_1"/>
    <dbReference type="OMA" id="HEIGKPD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000006">
        <property type="entry name" value="Expansion, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22742">
        <property type="entry name" value="EXPANSION, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22742:SF2">
        <property type="entry name" value="EXPANSION, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MH2" evidence="2">
        <location>
            <begin position="16"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009695-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="429" mass="48910" checksum="C66A7C2DF928D935" modified="2013-10-16" version="1">IQEVLNKWTQIDDEIWAKVIVFERNRRVAKAYARAPCLTINGANDGFDGMRIGLCGFDNPMRDTQTDEVKKRIDQGAKIKMDDYGNIFIKRYANSNVFVKSTALNPQDENSIGTDILKLQNQALEKGETYKLFDMRKFHHNMNAEFRRSYPDRQRLETQCLSALVFVKQENDILDCPVWILVINVVAMALLKSKLAPIPRNRPTPLDIKNRPRIPIPDEDPYSIAAANNNYDDLELENKLKLSRSKNGGEKERNGKDKKYDDPYYCGLRARVPNFVKSKSNSKGDKEKDKEKEKAQAAALANAAQQQQLSKKEQQQQARSQKKTSMIHHPSSSTSTTSSAAAAAALYHHQQQQQQQQLMAASGAHYPHYHANNHHLNHHIIWQSKSYDSGMDSPDNTIESPYNRIYGRLPLPTRGYVPGPRVAYIGEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Z8</accession>
    <name>T1H0Z8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="1">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02072151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Z8"/>
    <dbReference type="STRING" id="36166.T1H0Z8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009847-RA">
        <property type="protein sequence ID" value="MESCA009847-PA"/>
        <property type="gene ID" value="MESCA009847"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1121195_0_0_1"/>
    <dbReference type="OMA" id="DTWVENS"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="248" mass="28257" checksum="30B1596295BE1FB4" modified="2013-10-16" version="1">MIIQAIFIHIILLGSIFVIYFRSPVITGLSPIPNNQLRPPAKRLVLLVTDGLRSQSFFENDCQHVPNIKKLFLEQGILGISQTRVPTESRPGHIALIAGLYEDPSAVTRGWKNNPIDFDTVFNRSSMTYAWGAHDVLSIFSKITTDGRMLIDSYDHDWTSREVAKLSSWTRGKRAELQNEKRLVFFLHLLGLDSAGHIHKPGTPEFYENLHITDRGVLDIYEYFERVFPDKKTAYVLTSDHGMTDSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H179</accession>
    <name>T1H179_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H179"/>
    <dbReference type="STRING" id="36166.T1H179"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009933-RA">
        <property type="protein sequence ID" value="MESCA009933-PA"/>
        <property type="gene ID" value="MESCA009933"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1251917_0_0_1"/>
    <dbReference type="OMA" id="RIRTECY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF26">
        <property type="entry name" value="RH26557P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="27"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009933-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="221" mass="26212" checksum="252151D0F38AE07D" modified="2013-10-16" version="1">MTKVQSEDAGVVKRRIVAMCPPDFQRIRTECYHISSNNLTWLEAHFECKSRNGKLAEPTRIQDAAIRKFLLNKYGNMNKDVIYFGGMYDHRAQVWSWGVSGQKFNYSSFIGLKTYLKRKSLNHTCTVYNPNRKFRWTPRDCTENHRFICEHKMPKVTENNRDKVYQKWNMTYPNQIANEVVMEVRDDDRRNNSRSHINFIPLFGRNFTLGCQLISIRYIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1K5</accession>
    <name>T1H1K5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1K5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010068-RA">
        <property type="protein sequence ID" value="MESCA010068-PA"/>
        <property type="gene ID" value="MESCA010068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1095337_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="30637" checksum="84D47D184CF83AE5" modified="2013-10-16" version="1">MQNGKQFSFVFFDNRPQNFQSALLYIESIWGSPCHLTIVSERVQVRVNMKLIIIFCLLFQITIIKCDENEHVHLKVHIPEIRQKHTHFQKINKHHYHPKKVYYDYSFGPSIEFENFKTPFRKRKHISDYEIVPENDHFISHHSFYKPPEDNMLETIKFLQDFYDKHKKKSSASFAEPPEEDYSIDLSQLESSKYSVDEDGVDWKSSTTPHNYRHLYMDNIWTPYSGFNWKSSLMDRKRRHSNVNEPIGIRYKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2I4</accession>
    <name>T1H2I4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2I4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010432-RA">
        <property type="protein sequence ID" value="MESCA010432-PA"/>
        <property type="gene ID" value="MESCA010432"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471646_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="43"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010432-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10414" checksum="09FA2EA405823D46" modified="2013-10-16" version="1">MKECSRYVEKFPEKNPDFTIINGKLLKHAPNRNLTDDDWLLFVPAPMRERLLKENNDTSGHLGNRRTNLRICNKYSWPGMTRDNKHTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H370</accession>
    <name>T1H370_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H370"/>
    <dbReference type="STRING" id="36166.T1H370"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010689-RA">
        <property type="protein sequence ID" value="MESCA010689-PA"/>
        <property type="gene ID" value="MESCA010689"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2365573_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02679">
        <property type="entry name" value="MIT_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000006">
        <property type="entry name" value="Spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="10">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010689-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="11137" checksum="55AF3FE3E5C8B86C" modified="2013-10-16" version="1">MFSNKSPGPGDPLLARQKQHHRRAFEYISKALKIDEENEGHKELAIELYQKGIRELEEGIAVDCWSGSGEVYERAQRLHEKMQTNLSMAKDRLHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3J7</accession>
    <name>T1H3J7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3J7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010825-RA">
        <property type="protein sequence ID" value="MESCA010825-PA"/>
        <property type="gene ID" value="MESCA010825"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536308_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010825-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20182" checksum="C49576CA055E46E1" modified="2013-10-16" version="1">SLLRLVNVWTKPNGLGVNIAIIELALFTNRQRIPIFKLPILHGVELKLSNSATSLGVILYRKLCWQENIEERRKQVGSWLYNIVCMRAILICMLLVWWQALSKYVLFARLEGVERVCWYDKLSKVNYNHVSRGVKAINLVVKSIGVKNALHLQNVAVRETNKANLQESFQVQRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3M5</accession>
    <name>T1H3M5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4780 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3M5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010856-RA">
        <property type="protein sequence ID" value="MESCA010856-PA"/>
        <property type="gene ID" value="MESCA010856"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929972_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031961">
        <property type="entry name" value="DUF4780"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16012">
        <property type="entry name" value="DUF4780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4780" evidence="1">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010856-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14714" checksum="BBC5CF9DE26C8777" modified="2013-10-16" version="1">MVENAGELWKGAKLNVILLRELPSVPKLYLPVPVNVDTKDEDLLILKRQNPDIPMDSWNVLRIGKILNGRVFVTICIDKEGLAILEQKNHILHMAMGDLVDENPDDEENIIRELSNAKIDDVPEAPSSNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Q8</accession>
    <name>T1H3Q8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Q8"/>
    <dbReference type="STRING" id="36166.T1H3Q8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010892-RA">
        <property type="protein sequence ID" value="MESCA010892-PA"/>
        <property type="gene ID" value="MESCA010892"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_61_8_1"/>
    <dbReference type="OMA" id="IGCERIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003492">
        <property type="entry name" value="AGAP012735-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF448">
        <property type="entry name" value="FI20146P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="26"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="19663" checksum="04E802066D24A4DB" modified="2013-10-16" version="1">MRYNLDPFDEYSDEKLWGSLEEVKLKQVVADLPYGLQSKISEGGSNFSVGQRQLVCLARAILRENKVLVLDEATANVDPQTDALIQQTIRNKFAECTVLTIAHRLNTVMDSDKVLVMDSGQMVEFGTPFDLLTRSEKKVFYGMAQQTGKYMFDILFKVAEDAQDNCYKFKDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H451</accession>
    <name>T1H451_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H451"/>
    <dbReference type="STRING" id="36166.T1H451"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011052-RA">
        <property type="protein sequence ID" value="MESCA011052-PA"/>
        <property type="gene ID" value="MESCA011052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1139128_0_0_1"/>
    <dbReference type="OMA" id="WVTESGH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="244" mass="28027" checksum="0C0ED39DF30B4DD7" modified="2013-10-16" version="1">MRKLQFEFVRPELLVKSQFEKFKKVPTVKDNFPEKIISFANKVANMVTFLLSCGSEHHLKNPMLLEELIGKIPPSKKFEWAHYSYSLDRYATVVDFSRWVTESGHVILTSSKTICNFCNNEEHFIKDCAEFQNLTLEERWEHLKECKLCFSCLRKGHKIFNCKTNAILTIALNTIQHSCTTTRIKKPALKNSSKNNQLIIFLFGANGNRIRTLAFIDEGSTVTMINSSLARHLGVSGMKLHPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4E9</accession>
    <name>T1H4E9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4E9"/>
    <dbReference type="STRING" id="36166.T1H4E9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011158-RA">
        <property type="protein sequence ID" value="MESCA011158-PA"/>
        <property type="gene ID" value="MESCA011158"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_840175_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011158-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="331" mass="37042" checksum="6C97F33C3E2B117F" modified="2013-10-16" version="1">MKTAVKTVKEGSKTVYKDIKSKIRDINTNQSSHFHSHISNSHHSGMDTLSQTSMSCDFSSPSSPINSKKYFSQSSPVSNNSSDEVLSSNFVHLHPNGVSNDSPSQNLSPASSFGSLNFNLTQELQDHPLFQSPKVDRSLKPSSSFDMNQGKSGRCQHSSAPAIIPRSNTTAAKPAQKSTFFVNDSVIDKPPTKPTPPVPPRRHHNNVASSTNDLIKLETNCNNNVDRTDYNNLHTQLATKVSHELVLMKNNNVINNQHNPHMPLQRTSQQQQQRQISIMQQTQIHHHHFQHNNSIIPQMYHRNVVKEVVGDEDEAELPPIPTPRSKKEVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4V5</accession>
    <name>T1H4V5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4V5"/>
    <dbReference type="STRING" id="36166.T1H4V5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011323-RA">
        <property type="protein sequence ID" value="MESCA011323-PA"/>
        <property type="gene ID" value="MESCA011323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362102_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10694" checksum="55C3F1F8C044BCC3" modified="2013-10-16" version="1">MAESPKVQRNGQMRLMREESFSSYRSETEPELSMAGSPSRPETPGFPVTPRTPYGFSNGSISPALPPKSPNSQRRYNSSWSLRSQQSATSVLETRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4W4</accession>
    <name>T1H4W4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4W4"/>
    <dbReference type="STRING" id="36166.T1H4W4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011335-RA">
        <property type="protein sequence ID" value="MESCA011335-PA"/>
        <property type="gene ID" value="MESCA011335"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_938682_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051576">
        <property type="entry name" value="PX-Rho_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729">
        <property type="entry name" value="CDC42 GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729:SF10">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN CDGAPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="297" mass="33126" checksum="DBE0EEA6993D2026" modified="2013-10-16" version="1">MTDKNLAIVWAPNLLRSPALESGGVAALQGVGVQAVVTEYLIKNCRLIFDSVDDIMRNSFANAEHRMDSLTDCESLLIEQREHDQSLTFSERPKSLSAAGPKLISLEEAQERHSRLDSFDLNKAIPIPPSNPNIGSYIEVGGGPSSLPDKYHTKDARKADREKKVFSVRQLATKFEISPSAEQKPQNQKIKQLDRSAQITRSLDENAFVREFGSVKKIDDFTKSVQTISRPIDDDRKAKTLNLPKKIPGQVNLQKVEESASLDVKITPTNENPISLIQNNAISGVQLNRDRIERIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4X4</accession>
    <name>T1H4X4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074813">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4X4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011345-RA">
        <property type="protein sequence ID" value="MESCA011345-PA"/>
        <property type="gene ID" value="MESCA011345"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880592_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011345-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15260" checksum="291DFA2B2041301B" modified="2013-10-16" version="1">ASNKNSKTDTTEKVDTVPGLGFKDVEAAKQTLKILEGRDPEYQKFAVKGLIGSSKRVLAGTKNEDKIKAIKDAVQILEDFLDKFERDNVMRLNRAYLNYTTIEDLPAPKDKLAAEFLEAYGGSKAKGNYKHLRTMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H530</accession>
    <name>T1H530_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H530"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011404-RA">
        <property type="protein sequence ID" value="MESCA011404-PA"/>
        <property type="gene ID" value="MESCA011404"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485899_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011404-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10077" checksum="7E88D6EB91BC26EC" modified="2013-10-16" version="1">MIYPALKKGDRSDHRSQTTYALVTYQEIRISVNFHSKEGNSQELIIVMEVRSIALEGAKRQETWPATVEFTSKNYISILGILQKWNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5W1</accession>
    <name>T1H5W1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5W1"/>
    <dbReference type="STRING" id="36166.T1H5W1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012094-RA">
        <property type="protein sequence ID" value="MESCA012094-PA"/>
        <property type="gene ID" value="MESCA012094"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326674_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001197">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012094-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11303" checksum="8DDFA573F9FB9F6E" modified="2013-10-16" version="1">VPSQPSNFRATDIGETAVTLQWAKPSHSGENIVHYELYWNDTYANQDHHRRIPNTESTTLDGLYPDTLYYIWLAARSQRGEGATTPPIPVRTKQYGELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H664</accession>
    <name>T1H664_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7805 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H664"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012197-RA">
        <property type="protein sequence ID" value="MESCA012197-PA"/>
        <property type="gene ID" value="MESCA012197"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_367085_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000066">
        <property type="entry name" value="AGAP006059-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056707">
        <property type="entry name" value="DUF7805"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537:SF8">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25090">
        <property type="entry name" value="DUF7805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="705"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7805" evidence="2">
        <location>
            <begin position="458"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012197-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="760" mass="84947" checksum="0B4E9E2A0C0ECA88" modified="2013-10-16" version="1">DVLFSRRGRMGKIYSPRHTLPPNTTCTYHFHGYPGDLIWLSFTSFNLQILQQAIHDNNTLGRADLPPYITRLRMWDSYGTYVPIKQATTSSPSPHPSPVAAAEAGATIINSNNGGNYYYSPQNPKYGKSIRVNIDNAWNPVDTYIYGPTQSSIFNQENKYGGYFQKNQNSGGGSGLVSGVKESFNYIANSRFDKSIGGGRGDRNSIDLDTEHTTXMNAKSKRKLMLEIYDYETPKLCDHTAIGGQKLRPCSPLESYVSSGRDLXXXXXXXXXXXXXXXXFSLNYEFVDTEQGGDLWPGRKGEDPVPYLCSRIFKKRKGNVQAPRNTFLYGRGGAKNLTCLYRFEAPVSHRVKLFLHNVSFGEGSSCTTDTDLHTGRPKCNQLDPDGRITELKLFDVPFRDVKIPLGCFCDNTSSLYNTPFTIISNSRIMELTFTVSRLNISEDFADVYFYASYEFSRVSECNKELKLHGGGGEEEIKYPLKSQDASCEGLSWYIEAQHQDKSLFVQTWGTYLPTEPTSEDSMRCHTKNRLMVYTGRPLRLMKVVCPAQAGPRPPSLHIFSEDWTTGQPLFTNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRNCPSGVDESEVECGTARKLLELPGGVFAAFGCIAAAITACCFFCLFSLCRKRKKPIKNGGVLNGTLKKDFKKDSLFIDPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H665</accession>
    <name>T1H665_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H665"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012198-RA">
        <property type="protein sequence ID" value="MESCA012198-PA"/>
        <property type="gene ID" value="MESCA012198"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145022_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012198-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27090" checksum="5D0A87ED5B4ECD56" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLNDNTSDQIVNGNNNIYXTIITITPKTPQRARSGRGERSLLPCEVCGKAFDRPSLLKRHMRTHTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDPQDECVRCGKQFLTNGNNTQGVCLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6G9</accession>
    <name>T1H6G9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Metalloendopeptidase</fullName>
            <ecNumber evidence="12">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="12"/>
    <dbReference type="STRING" id="36166.T1H6G9"/>
    <dbReference type="MEROPS" id="M12.011"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012302-RA">
        <property type="protein sequence ID" value="MESCA012302-PA"/>
        <property type="gene ID" value="MESCA012302"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_366651_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF37">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="16">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="324"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="13">
        <location>
            <begin position="329"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="539"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="13">
        <location>
            <begin position="583"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="13">
        <location>
            <begin position="681"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="215"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012302-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="761" mass="85644" checksum="28A8F3C9D6DF8D9C" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDHRKIQSDTKDIEDDTRYYFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCCSFVGKRGNGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPSGKYEVDSLGQPYDYNSIMHYARNTFSKSSYLDTILPVDRPNSKRPEIGQRVQLSEGDIAQAKLLYKCPSCGRTFQENSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPNDQLKFINTFCGSDIPPVITSDANILHIEFRSDKSIQKTGFAAIFQTDVDECLDNNGGCQHECKNTIGSYICLCNNGYTLHENRHDCKEGACKYEITAPYGNIYSPNYPGLYPAHLDCVWHFTVIPGHRIKLIFNFFEVESHQNSSLLGVFCSDKLPYPISASTNEMYMVFQTDKNKQRGGFSATHSTACGGFLRATNHIQQFYSHARYGNANYDENMNCEWRIQAENGKNIQLDFLFFQLESTINCTYDFVEVEDDISGPSYGRFCGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6K6</accession>
    <name>T1H6K6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6K6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012339-RA">
        <property type="protein sequence ID" value="MESCA012339-PA"/>
        <property type="gene ID" value="MESCA012339"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913898_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012339-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="305" mass="34927" checksum="109047D2280C257B" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDDEDGDNKDVEEEPLYVNAKQYKRILIRRQARAKLESRIPKERSKYLHESRHRHAMNRVRGEGGRFHSQSTNGNQHDQLPSIQSIRNVTKAPPKXXXXXXXXXXXXXXXXXXXXXXXXDVNKNLERIGYNIGMRLIEDFLSRTAAPRCLEMRDTADRIQQAFRIYLNIQPAISNWSSSSDEFSLIFENNPLIDFVELPPELNNLKYSAIICGCIRGALEMVQLEVKTTLVQDQLKNDNCTEIRVKLVRRLEDAIPAGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Z8</accession>
    <name>T1H6Z8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tektin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6Z8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012480-RA">
        <property type="protein sequence ID" value="MESCA012480-PA"/>
        <property type="gene ID" value="MESCA012480"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033588_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF7">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012480-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="362" mass="41517" checksum="6F6011EF25C067B1" modified="2013-10-16" version="1">VAELDRWRSAIERTTERINREFDMLKEEKAITEIEIELMVTPFNVVSECLAMRDCRLGSEITYDEADTELKKELAILENNQKLLREQCQMTWEKMNRLAEVRFKLNLEIDNKREAQDIDVAQLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEQSLRDKTDGCKLAQTRLENRAQRAGKELVLDEPYFKLVDEVNKLDKIRKALQDQIVASKTNFTLLQEHAQRVNVDLDNKQHSLMTDIRGLDLRARLKASGALGPGADCKKKTQLDRNIELTQMEKEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H721</accession>
    <name>T1H721_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H721"/>
    <dbReference type="STRING" id="36166.T1H721"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012503-RA">
        <property type="protein sequence ID" value="MESCA012503-PA"/>
        <property type="gene ID" value="MESCA012503"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631258_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303:SF31">
        <property type="entry name" value="CYTOCHROME P450 307A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303">
        <property type="entry name" value="HEME-BINDING MONOOXYGENASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18513" checksum="13377ECD9DADEA3B" modified="2013-10-16" version="1">YAVDFLPWLAPFYHNHMNRIVHWSSTIRTFILERIINDRERNLDIDEPEKDFTDALLKSLIEDEDVSRDTIIFMLEDFIGGHSAIGNLVMLALAYVVRNPEIGKQIQAEIGKITDNKRSVSLYDIESLPYTVATIYEVLRYSSSPIVPHVATEDAAIAGFGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H774</accession>
    <name>T1H774_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H774"/>
    <dbReference type="STRING" id="36166.T1H774"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012612-RA">
        <property type="protein sequence ID" value="MESCA012612-PA"/>
        <property type="gene ID" value="MESCA012612"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042284_2_1_1"/>
    <dbReference type="OMA" id="NTENYCS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12876">
        <property type="entry name" value="SPRY_SOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035754">
        <property type="entry name" value="SPRY_SPSB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF12">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012612-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="16868" checksum="3B72E2C6A03364E8" modified="2013-10-16" version="1">GCEDNWCWSNVHRSYEVNISGTNASVAHFHPNWSKGTAGVRGTRPLNNGRYYWELQLSERIFGTSMMFGIGTEKTRLHADSFINLLGEDTNSWGLSHKGVLWHGGVALMYTKRFRENTPTTIGILFNGIEGTLTYYKDGVCLGVAFSGLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7C0</accession>
    <name>T1H7C0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7C0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012658-RA">
        <property type="protein sequence ID" value="MESCA012658-PA"/>
        <property type="gene ID" value="MESCA012658"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1334824_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007167">
        <property type="term" value="P:enzyme-linked receptor protein signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11758">
        <property type="entry name" value="SH3_CRK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035457">
        <property type="entry name" value="CRK_SH3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051184">
        <property type="entry name" value="Tyrosine-phos_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969:SF5">
        <property type="entry name" value="CRK-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969">
        <property type="entry name" value="SH2-SH3 ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="80"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23489" checksum="B54D17917E56CCA5" modified="2013-10-16" version="1">WYFGALSRQEATEMLQNQESGVFLVRDSEGAKVSHYIISSVXSMDSDQMGDQIFIDLQEILSFYKKHYLDSTNLIRPAKKTFEQVLAKFDFEGNDLEDLPFKRGEVLQIISKDEQQWWTAKNSLGKIGQIPVPYVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7D6</accession>
    <name>T1H7D6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosducin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7D6"/>
    <dbReference type="STRING" id="36166.T1H7D6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012673-RA">
        <property type="protein sequence ID" value="MESCA012673-PA"/>
        <property type="gene ID" value="MESCA012673"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072604_0_0_1"/>
    <dbReference type="OMA" id="VQYRYGN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Phd_like_VIAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051498">
        <property type="entry name" value="Phosducin-like_chap/apop_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809:SF3">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosducin" evidence="2">
        <location>
            <begin position="34"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012673-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22817" checksum="BB2426D4723F0BFC" modified="2013-10-16" version="1">DPNEDTEWNDVLRAKGILPPKQKEAEISEDQIVSMIDEAIKKRTDAGGESRKDIGDMDLDELDELEDSEDEAVLEEYRRKRLAEMKIFADKARFGSVREISGQDYVEEVNKAGQDIWVVLHLYANGIPQCNLIHHYMNDLAVRFPQTKFLRSVATTCIPNFPEKNVPCIFIYYEGQMRKQFIGAVDLRGDKLTKDEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7G3</accession>
    <name>T1H7G3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">High mobility group protein DSP1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7G3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012700-RA">
        <property type="protein sequence ID" value="MESCA012700-PA"/>
        <property type="gene ID" value="MESCA012700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_851463_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012700-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="36440" checksum="D19D0FCADD65BB17" modified="2013-10-16" version="1">HPAFDQQLAQQQQQQSAAQQQQQQQQQQQQQHQVVVQQPQQAHTNDARNNCTTSGVATQQLFSYKMASSFQNPATTMSQVVATSNSGGAGNSTGYDYRTINMAQAAAAAANPGGAAQWWYAGANQNQIDNNSTAQQQNQQQQQQHQQNMQQQQQQQQQQQQQQQQQNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9X3</accession>
    <name>T1G9X3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">POU-specific domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9X3"/>
    <dbReference type="STRING" id="36166.T1G9X3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000014-RA">
        <property type="protein sequence ID" value="MESCA000014-PA"/>
        <property type="gene ID" value="MESCA000014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013065_0_1_1"/>
    <dbReference type="OMA" id="MMSHHHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF70">
        <property type="entry name" value="INHIBITORY POU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="8">
        <location>
            <begin position="84"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="19970" checksum="D04399D785F189C7" modified="2013-10-16" version="1">MHHTMDGLDMLDPTGSMTTLTPMADTPISSSHHQLHGSYHGMNHMMSHHHAAQLGGHTPGHHPHPSAHHPAMAAAVAAAGLHPDTDTDPRELEAFAEKFKQRRIKLGSVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQAKNKRRDPDAPSVLPAGEKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA12</accession>
    <name>T1GA12_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041002">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000055-RA">
        <property type="protein sequence ID" value="MESCA000055-PA"/>
        <property type="gene ID" value="MESCA000055"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457372_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000055-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10329" checksum="2D076C256D8D898B" modified="2013-10-16" version="1">MVIQDKRSVLSRLLMRRLLNSLSDLSRHLFPCPESKTDILAVARILLYKKEKEALSAIVRFFVITYKESTIHRLARRGGVKRISGKRHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAA1</accession>
    <name>T1GAA1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAA1"/>
    <dbReference type="STRING" id="36166.T1GAA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000151-RA">
        <property type="protein sequence ID" value="MESCA000151-PA"/>
        <property type="gene ID" value="MESCA000151"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000151-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="14838" checksum="BC39116D482DC16F" modified="2013-10-16" version="1">MKGSPNSSDRDAGFCSGSEAGDDLSIDNSRLEPCSPTNTSEDSIEVKVPPTSGRNKRKSTEPSKVVCREQETGPLKKRIRYNTSSSSESSFRPWDEIKQQQQQQQHHHQIPRLEIPVPMPMPNPAEIFLRHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAH8</accession>
    <name>T1GAH8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAH8"/>
    <dbReference type="STRING" id="36166.T1GAH8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000235-RA">
        <property type="protein sequence ID" value="MESCA000235-PA"/>
        <property type="gene ID" value="MESCA000235"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608751_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8885" checksum="E306DD935FC063CB" modified="2013-10-16" version="1">MEILGYDEGICPHTNDDELRSCIKDHQAILEGPSSSDSDLIPEPGVCGNVITNKIYGGVETRLDEYPWMALIEYQKRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAX1</accession>
    <name>T1GAX1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAX1"/>
    <dbReference type="STRING" id="36166.T1GAX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000387-RA">
        <property type="protein sequence ID" value="MESCA000387-PA"/>
        <property type="gene ID" value="MESCA000387"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124090_2_0_1"/>
    <dbReference type="OMA" id="GYKYFRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF67">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN TIWAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="1">
        <location>
            <begin position="30"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000387-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13072" checksum="FDE743E52E0E88CF" modified="2013-10-16" version="1">MKGTQFGLSVALFTPRIPCVAAASRYTAPVHIDVGGSIYTSSLETLTKYPDSKLAKLFNGSIPIVLDSLKQHYFIDRDGGMFRHVLNFMRNSRLLIADDFPDLELLLEEARYFEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB35</accession>
    <name>T1GB35_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mucin-19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB35"/>
    <dbReference type="STRING" id="36166.T1GB35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000456-RA">
        <property type="protein sequence ID" value="MESCA000456-PA"/>
        <property type="gene ID" value="MESCA000456"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388972_0_0_1"/>
    <dbReference type="OMA" id="HGTVTSH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588154" description="Mucin-19" evidence="1">
        <location>
            <begin position="21"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000456-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="69455" checksum="22B472EB5810B21D" modified="2013-10-16" version="1">MKMKAKILIVIVAVLGLTFADVSHLQPHNGYLPPVGSQTHETTSVVKVSSHNTHTTAGPVVTKTIHQGVPAIVTHHNTVAPSVLKTIETHSVGGFGLLGKAAGLGSTVTSSPIVSKTVSKTVTTENVGAHSAPVGSYQKSVTTHIGGAHSVSVSPAPVVGHIGSIGHLGSHGGSVTKTVSVTSAPVVTKTVTSHVTAPVVTKTVSSYGSVGAHSHPISSVTKTVTTSHGAGHLGGHGTVTSHVSAPVVSKTITTSHGGVGLLGGHGTVTSHVSAPVVTKTVSTKTITSSHGDGLLGGILSHGGHGTVTSHVSAPVVSKTVSSYGSVGAHSAPISSITKTVTTSHGAGHLGGITSHVSAPVVSKTITTSHGVTTSHGDGHLGGIISHGGHGSVTSHVSTPVVTKSVSSYGSVGAHSAPVSSITKTVTTSHGAGHLGGISSHGGPGTVTTHVSAPVVSKTITTSHGGIGNLGGHGTITSHVSTPLVSKTITTSHGGIGNLGGHGTVTSHVSAPLVTKTVSTKTVTTSHGDGHLGGVLSHGGHGTVTSHVSAPVVTKTVSSYGSVGAHSAPVSSITKTVTTSHGAGHLGGIASHGGHGIVTSHVSAPVVTKTVTTSHDGHLGGHGSVISHVSAPVVSKTVSTSSQGIGHLGGLVHGAVTSHVSAPVVSKTITTHGGSLGHGSVTSKTVTTNVGHTFGTQITTMLLLLPMSNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBA9</accession>
    <name>T1GBA9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Diphthine methyl ester synthase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02011729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBA9"/>
    <dbReference type="STRING" id="36166.T1GBA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000536-RA">
        <property type="protein sequence ID" value="MESCA000536-PA"/>
        <property type="gene ID" value="MESCA000536"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_1_1_1"/>
    <dbReference type="OMA" id="KECINAE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000536-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18533" checksum="522E32544E338EB6" modified="2013-10-16" version="1">MNAVGCCGLQLYNYGETISIPYWDDTWKPDSFYDKIKQNRQHKMHTLCLLDIKVKEPTLESLMKKKREYMPPRFMSVAEASTQLLEIAKNRNEPEILNEDTLVVGLARVGHDDQTIKVCTLREMAESDLGGPLHSMVIPSNHLHALEIDYLQLFSENKIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBX9</accession>
    <name>T1GBX9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000770-RA">
        <property type="protein sequence ID" value="MESCA000770-PA"/>
        <property type="gene ID" value="MESCA000770"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443349_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000770-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10599" checksum="F0BBE030829AAFC3" modified="2013-10-16" version="1">MFKTAATEFAFLIFRNTRRSRINCSQFRNLSTKVQPNLVRKMSKYSIVEKGAPNSTDFSIYFKNEHGNACLHFMTFHCTPMRRRPSTIWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDD1</accession>
    <name>T1GDD1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor 5A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02142990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDD1"/>
    <dbReference type="STRING" id="36166.T1GDD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001316-RA">
        <property type="protein sequence ID" value="MESCA001316-PA"/>
        <property type="gene ID" value="MESCA001316"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_2_0_1"/>
    <dbReference type="OMA" id="HVIGICD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0385">Hypusine</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001316-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7706" checksum="1EF5307BEC91BE72" modified="2013-10-16" version="1">MDVPHVKREDYQLTDISDDNYLTLMSDNGDIREDLKIPEGDLGNSIRTDFDGGKDLLCTVLKSCNEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDE7</accession>
    <name>T1GDE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02154881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDE7"/>
    <dbReference type="STRING" id="36166.T1GDE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001332-RA">
        <property type="protein sequence ID" value="MESCA001332-PA"/>
        <property type="gene ID" value="MESCA001332"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888147_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF2">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="50"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001332-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15406" checksum="A4AE07FF7E29AD4E" modified="2013-10-16" version="1">MDSRDKRQLESLQITHILTVLEHKEVKDKNSNENHMFVEADDKSTEDLSKNFQKCNDFINSGRQSGGNVLIHCRCGKSRSVTIATAYIMKQLECWEKNGNVILRKVDGDLEFCADVLKNTDCSEGNDDKCFFVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDH2</accession>
    <name>T1GDH2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDH2"/>
    <dbReference type="STRING" id="36166.T1GDH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001359-RA">
        <property type="protein sequence ID" value="MESCA001359-PA"/>
        <property type="gene ID" value="MESCA001359"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008954_1_3_1"/>
    <dbReference type="OMA" id="SLWVDFY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17336">
        <property type="entry name" value="MFS_SLCO_OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF159">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 74D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="9">
        <location>
            <begin position="339"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001359-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="509" mass="55488" checksum="BB91E8DC79CD6054" modified="2013-10-16" version="1">MDCLWNGIVLHSAMVFSLPHFLYGDKTLKSLDTSEDFSSSMQNVSTAYDVCSISPIFDEGTCKTETIQESSKVSFVALTIFFLGLFFCGVGQVAIGTLGIPYIDDNVASRESPIYLGITIGVKILGPTIGFVLGSYCIKIFVDLSTPSFGPDDPHWIGAWWLGPIIISSLMLLASLAMFSFPKELKKNTKGKPLSNGNGMDKKIIIVSKEAEKEEKPKLRDFPKTLKRQFKNDILMFRTASSVFHLMPISGFYAFLPKYLESQFYIPMATSTLIIAFGGILPMGFGVFISGITFTKIQPSARKVAAWIAMCALIFSAGSLILMFIGCGNDVSPDSISRHMKMNLCSNVTCDCDKNKFSPICGVDGQTYYSSCHAGCSEVKSNGTDTIFKNCSCIDAPEQTAKIGRCDSNCRNFIVFVCIFSVCVFIHSTSEVGGYLLIMRCTDPKDKALALGLIQSAIGLLGNVPCPLIYGAVVDSACLVWTKICGKLGACIVYDDNCLGDTFWVLPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDJ6</accession>
    <name>T1GDJ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="1">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02025498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDJ6"/>
    <dbReference type="STRING" id="36166.T1GDJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001384-RA">
        <property type="protein sequence ID" value="MESCA001384-PA"/>
        <property type="gene ID" value="MESCA001384"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024661_0_2_1"/>
    <dbReference type="OMA" id="CNVRRIY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF24">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="2">
        <location>
            <begin position="30"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001384-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="473" mass="54758" checksum="9859EC00B534C5BE" modified="2013-10-16" version="1">MKPTNMIEEFYENREIFITGGSGVVGKGIRKERNKAEERLKLVKESKIFNVLKLVKPNELDKLTAIPGDIMSPLLGISEANLKVIQNVSIFIHSAATVRFDEPLRKAIRVNIGGTYESMKVAQTLNNLEIFAHVSTFYSNPYLKYVDSKLYPPVMDWKTVLKLANSNIPDEVIDILTIKYTSMFPNTYTFTKSLAEHVVNEHRHLFPTLIYRPSIVIFSLKEPEPGFVDSYYGLTGITTANGTGLMRTLYGYKNIRLDKCPLDIGTKVLLLQVFKEAKTKISYVPREETKVAMFSSTMVDRTTMLEEVRLGKEVYRSWEKNPFNKTVWYPGLKVTNLLLRLFGIQPVVMSIQRKLFLAIRSMEHFVFNVYESPGITDMDLLLNISAKTSFAFPEVKSCLNERTKKRVYNLFTISSKKFILNEDSKHLNKARIWIKFLKYLDLFTHWYIRYWIAKHVLIFIYNYIKAQLPLESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEH3</accession>
    <name>T1GEH3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001735-RA">
        <property type="protein sequence ID" value="MESCA001735-PA"/>
        <property type="gene ID" value="MESCA001735"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111644_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001735-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="12859" checksum="F33B713CECA41D10" modified="2013-10-16" version="1">MQYDLVPGQPNEILIYLAAVGSNSGGRIPILLRADSNTVKTLEAKNFEIGFCLRTVVLRVIKSNLKEMSNKEVSVEQLQADEFFDLSSLFNTGLDLDKESETDKMDVVEGEDQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEK1</accession>
    <name>T1GEK1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02066948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEK1"/>
    <dbReference type="STRING" id="36166.T1GEK1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001766-RA">
        <property type="protein sequence ID" value="MESCA001766-PA"/>
        <property type="gene ID" value="MESCA001766"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1009318_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF89">
        <property type="entry name" value="GH15894P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001766-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="276" mass="31760" checksum="944E124D6189CC84" modified="2013-10-16" version="1">MNKTIIVPNTIMLTIAAFVHEHVNNFFARSAINIMLMKEPIFVTKTIHQFLWDNTSPLVELVKPFIPSTLFPRTNIGMLYNYFYPEKMRFNVNIGTKHGFKEFFKINSLNYKPYIIGFKDSDYEDCKIYSIQEGRDGTGLPPYFNKDEDVINAYTFMIPRTYPTIYSKDIRIGGLDIYDCRFQEGIYKRLNNSQDECLGRTGSSELPEGLIDTSKIVFGVPFALSPPHFYGYKGVWNNYIRGLNGSDEEHGRSIGTQIYEVLHDVYDLLPNADASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEY9</accession>
    <name>T1GEY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEY9"/>
    <dbReference type="STRING" id="36166.T1GEY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001916-RA">
        <property type="protein sequence ID" value="MESCA001916-PA"/>
        <property type="gene ID" value="MESCA001916"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1456027_0_0_1"/>
    <dbReference type="OMA" id="RICCHTD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF15">
        <property type="entry name" value="IP01243P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001916-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="22095" checksum="1F91FDFA335C5EEA" modified="2013-10-16" version="1">MINDKLSIFLKQLKHKKISRKVHINTHTGNKPYHCKFCDSKFTTPGEVVRNVQYRHTLERPHKCIKRDYGSVELSKLKRRICCHTDEGPYQCTYASPETDKMKRHLRIHTGKNHMNRHMDSHMLIHTDEKPFKFDKCDKKLLNRHENVYYNKDYVAPEGKTKDPEMPSLSEILCPQRQPYQAHVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFT1</accession>
    <name>T1GFT1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFT1"/>
    <dbReference type="STRING" id="36166.T1GFT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002231-RA">
        <property type="protein sequence ID" value="MESCA002231-PA"/>
        <property type="gene ID" value="MESCA002231"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270473_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047163">
        <property type="entry name" value="ASPP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131:SF10">
        <property type="entry name" value="ANKYRIN-REPEAT, SH3-DOMAIN, AND PROLINE-RICH-REGION CONTAINING PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002231-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11829" checksum="281FB5FF43AF6917" modified="2013-10-16" version="1">MHHRRKKNKHTIQLQAVDGISSSLHKVSQFSDQQPGNIVPGRPSQAELRAMALRQQQQIDSQHQLLAAKEQRLRFLKAQEVRGAAAAAEGERLRRLRERKYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFU1</accession>
    <name>T1GFU1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFU1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002241-RA">
        <property type="protein sequence ID" value="MESCA002241-PA"/>
        <property type="gene ID" value="MESCA002241"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123903_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002241-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13011" checksum="EE8748AB0D9253B2" modified="2013-10-16" version="1">MAKPINSQTKFFYWKNELKHCLLAEYIECSPTDLKSSSSNPTNLVRSYQISVRVSANINTGSLIFNKSHQILAYVYDIVRSSVNVVESFLRLERAVESVGLQINENKTKYMLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFW2</accession>
    <name>T1GFW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CDR ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFW2"/>
    <dbReference type="STRING" id="36166.T1GFW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002264-RA">
        <property type="protein sequence ID" value="MESCA002264-PA"/>
        <property type="gene ID" value="MESCA002264"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640971_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="9067" checksum="25F223B596E10C5F" modified="2013-10-16" version="1">MLYANEAMTVAQWRGVENITCFQESTELPCLANGKDVLDKYSFSEGRVYTDIFSIVVIYFIFHLLAYFCLWRKARRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG33</accession>
    <name>T1GG33_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG33"/>
    <dbReference type="STRING" id="36166.T1GG33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002339-RA">
        <property type="protein sequence ID" value="MESCA002339-PA"/>
        <property type="gene ID" value="MESCA002339"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1410312_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF38">
        <property type="entry name" value="SYNAPTIC VESICLE 2-RELATED PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002339-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22134" checksum="C46ECF4F87B24157" modified="2013-10-16" version="1">MGISPEITGCQVGWSACAVENIILGFWLFAFPESPKFHYEIGETDKALNILQHMYAVNTNRSPDDFPIKTLKEPEKSESEQRLYGDRTVRQLRIRRPRELALLLKEIWTQTKNLCKKPHLRNTALTCFIQFGLTMSYLMDSAYPMHPVVFHSLVYLCTYLDSAYLDSVSEFISFPTGLKPTKLAVLNGDRLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGD9</accession>
    <name>T1GGD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGD9"/>
    <dbReference type="STRING" id="36166.T1GGD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002456-RA">
        <property type="protein sequence ID" value="MESCA002456-PA"/>
        <property type="gene ID" value="MESCA002456"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037367_0_0_1"/>
    <dbReference type="OMA" id="KVHCYCD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF370">
        <property type="entry name" value="FISH-LIPS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002456-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34767" checksum="3E4CF6980C5B1E9C" modified="2013-10-16" version="1">MFPPTYAKLVCHCTPDDEKLPANKMECWVFQKGLLLNDTIWTGFKAQKSLGHLKILVQDGGELKYVPTKYLEQLANLTSIQIDYSQIRIIKSFAFANLPNLREITLTNNQIEMIDKDGFANHIFLKEMDLRHNQISEIDRYAFRNLPILKKLILDENNISTLHEELFNELGKLEELSMAHNLISVLTGDMFKGLGNLRILKMTGNNLNFIGDTVFAELWSLQELELDDNHIERISERALDGLNTLKTLNLRRNLLKKLDSGIFRGTPALLSINLQDNPLETLTYFSFQPIMIIWLIVLVNYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGK3</accession>
    <name>T1GGK3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposable element P transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGK3"/>
    <dbReference type="STRING" id="36166.T1GGK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002524-RA">
        <property type="protein sequence ID" value="MESCA002524-PA"/>
        <property type="gene ID" value="MESCA002524"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_440253_0_0_1"/>
    <dbReference type="OMA" id="SEMTAIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposable element P transposase-like RNase H" evidence="1">
        <location>
            <begin position="64"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Transposable element P transposase-like GTP-binding insertion" evidence="2">
        <location>
            <begin position="189"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002524-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="70774" checksum="75FE0F8B5115AEEF" modified="2013-10-16" version="1">MVSASNTVHWSNDDVSEAISLHAAGPKLYRLLRSKGLPLPGESTLRRWVSNVKIVPGKIGLSFELLKHISLDPVDRVCVLIADEMKVRECYEYDRATDTLIEPSATVQVVVVSGLFINWHQPIYFGFNTDLTIELIFDLMKELHNIDFEVRALNFDLGPKNRSIISSLGPVFINPFDNQRTFVFADSPHLVKLMRNHVVDNGIVTKEGHLVDKQPLVKLMELTKKDSVKITYKVTEESLLVKGFGKQKVKLAAQLLSRTNSAALQRTVSLGLVDEAEKEKWLVVADVFELTNDWFDVFNSSKPRRDNRERLWGFGLSLDVQEEILQRSIAFFESMNCRVKNRSGHFSLASMAPFQKGIIMNCKALPLLFKDMEQKYGIEYILTRRLNQDYLEALFGQIRAMGFLHNHPSPLEFCSRLRKYLLGKNKKILSMSSNVELDYDLINNKENIAMTNQTSNEQEPSIQLPLTAELFSSIQCEEQEDDEDVESEEIVIFKTTVCNMLQLRKSEDLGSPSSEQSCSTDKLTWVDFLSEGGLYKPHTWVLNSVKLLHEVFIKQNGDSLSPGANYIGRMLDASCHVRGSTNMKSLFFRCVMYFKIRQLTKHGVMTKSRNGCNQKMKKMTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHA5</accession>
    <name>T1GHA5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02188105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHA5"/>
    <dbReference type="STRING" id="36166.T1GHA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002795-RA">
        <property type="protein sequence ID" value="MESCA002795-PA"/>
        <property type="gene ID" value="MESCA002795"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_0_0_1"/>
    <dbReference type="OMA" id="FCSAGDV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF176">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002795-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="374" mass="42038" checksum="651D2CDD5FF984B7" modified="2013-10-16" version="1">MVFKMGLHPRIYATFQYSMVQYFELFGNCMFLSGMVAMIMLRTLHKDIARYNQMESGEDAQEEFGWKLVHGDVFRPPRKGMLLAVFLGSGVQVFFMSLITLAFACLGFLSPANRGALMTCAMVLYVALGTPAGYVSARIYKSFGGIKWKSNVLLTSMVCPGVVFSLFFVMNLVLWGEGSSGAVPFSTLVALLALWFCVSVPLTFVGAYFGFRKRALEHPVRTNQIPRQIPDQSIYTQPIPGIIMGGVLPFGCIFIQLFFILNSIWSNQMYYIFLDLSDHMLRNYNPSMLLPPLCGRLPLVVGSFLTSGCTAVYLFVYCCHYFATKLQIKDGASTFLYFGYTAIMVFLFFLLTGTIGFAACFWFIRKIYSVVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI14</accession>
    <name>T1GI14_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Toroid-shaped homodecamer, composed of two pentamers of five dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02014148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI14"/>
    <dbReference type="STRING" id="36166.T1GI14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003081-RA">
        <property type="protein sequence ID" value="MESCA003081-PA"/>
        <property type="gene ID" value="MESCA003081"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189391_0_0_1"/>
    <dbReference type="OMA" id="FGHEKCT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="8">
        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003081-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10351" checksum="E5A880A404B3EA29" modified="2013-10-16" version="1">MLKKSHEKCTFHHDLELDHKPPTREALLPDMAHSYRMLLSGLGENPDRQGLLKTPERAAKAMLFFTKGYDQTLEDSAYPWTRNIVLIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI30</accession>
    <name>T1GI30_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI30"/>
    <dbReference type="STRING" id="36166.T1GI30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003097-RA">
        <property type="protein sequence ID" value="MESCA003097-PA"/>
        <property type="gene ID" value="MESCA003097"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326671_0_0_1"/>
    <dbReference type="OMA" id="WNCDESL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003097-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11327" checksum="F4CD2853D4C9CE50" modified="2013-10-16" version="1">MPAENLEEQGLEKNPNLELAQWKFLLTLKEHQNNTALKAKLLDAIKNDTMAPWYEIVCSELNWPVDQSLLSKMKDENAKKLEELDATIADAEQNLGEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GID5</accession>
    <name>T1GID5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GID5"/>
    <dbReference type="STRING" id="36166.T1GID5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003208-RA">
        <property type="protein sequence ID" value="MESCA003208-PA"/>
        <property type="gene ID" value="MESCA003208"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1772757_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003208-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16123" checksum="2F04A3963FC62C80" modified="2013-10-16" version="1">MSTKSITPYLFIWFLVQRLELEKNEEVVVGGSFENESDTNSTGTPKTIVPGQPLRREGSDLDKKKKPVSPAPAKQPGVTNILARTSSSSSTGSHASSQRQNSQSSLFDHFASQAKELVRETTRQSSQEGLLAHVDKADEKVLHSLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIR3</accession>
    <name>T1GIR3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003348-RA">
        <property type="protein sequence ID" value="MESCA003348-PA"/>
        <property type="gene ID" value="MESCA003348"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253114_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12287" checksum="C30A08096A066354" modified="2013-10-16" version="1">MVLVCGSLSNFHALLVLNMRRVFFLMLGVETVLFKKENIKTCKNNFVDEFLRIPQWFNGCNHTYIQHIFKENIIFDTLPGNIVEFVKKEYKKSLECTQLKIEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ09</accession>
    <name>T1GJ09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02167202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ09"/>
    <dbReference type="STRING" id="36166.T1GJ09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003448-RA">
        <property type="protein sequence ID" value="MESCA003448-PA"/>
        <property type="gene ID" value="MESCA003448"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657320_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003448-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="9013" checksum="03DEBAA3F0AC184C" modified="2013-10-16" version="1">MRDAMVKMWVNFANFGNPTPDKKLSKWEPTTKYPWNYAHLGGIDESGFFVLKNAEGYARDRLELWNKLRPHRENDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ35</accession>
    <name>T1GJ35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003476-RA">
        <property type="protein sequence ID" value="MESCA003476-PA"/>
        <property type="gene ID" value="MESCA003476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309194_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11524" checksum="8932CE46ECEC3B8F" modified="2013-10-16" version="1">MKVNLLIIYKTSHLVTVTSWSFDNQLHTSFNCLSLDYCYKKPDIQQSYLHYWLEEVLSIKSNCLILTDDTFWAVLQETEDFISEGMGIGTLEPNLIGVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJD6</accession>
    <name>T1GJD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037638">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJD6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003582-RA">
        <property type="protein sequence ID" value="MESCA003582-PA"/>
        <property type="gene ID" value="MESCA003582"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608792_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003582-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9251" checksum="ECB9037D48DBBA1E" modified="2013-10-16" version="1">MDVQTEIRRKLGLAISYKPPDGGIVVSASRWWITSQLRKRPFIISKVESALVSQKDYKKNKWCSLRPIEIVRDFLVLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJV8</accession>
    <name>T1GJV8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02039227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJV8"/>
    <dbReference type="STRING" id="36166.T1GJV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003763-RA">
        <property type="protein sequence ID" value="MESCA003763-PA"/>
        <property type="gene ID" value="MESCA003763"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106531_2_1_1"/>
    <dbReference type="OMA" id="MVISSYF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF35">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003763-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14438" checksum="C586A599AD5515CD" modified="2013-10-16" version="1">METAVDFERALEHADFGKFNIFLIIFSGFVMTNAHLETASIGYILPTSQCDMDWSIQQRGILSATGFFGMVISSYFWGVLSDTTGRRRTMRITLILGFICSIISSFSINFWMFFTMRLLNGICISGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK83</accession>
    <name>T1GK83_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003901-RA">
        <property type="protein sequence ID" value="MESCA003901-PA"/>
        <property type="gene ID" value="MESCA003901"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898624_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003901-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15918" checksum="CF1D1BE0C0F09522" modified="2013-10-16" version="1">MDGSNVLKRCEHLSTKVIGLQKPRRASVWRVQEHSEAVSKTRKSKLRGLSKRSFEKQQFEDLRFMRDRNQASKLYQNVNKERKNYTPVGSSCKDRNANLITNRKEILMRWEEFSSDRLMTISRTSKNRLLFPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKC9</accession>
    <name>T1GKC9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphofructokinase</fullName>
            <ecNumber evidence="4">2.7.1.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02190937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKC9"/>
    <dbReference type="STRING" id="36166.T1GKC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003951-RA">
        <property type="protein sequence ID" value="MESCA003951-PA"/>
        <property type="gene ID" value="MESCA003951"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011053_0_0_1"/>
    <dbReference type="OMA" id="EWQDQMC"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="15">
        <location>
            <begin position="18"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="15">
        <location>
            <begin position="222"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="15">
        <location>
            <begin position="393"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003951-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="757" mass="83758" checksum="88E76EA2D229B3ED" modified="2013-10-16" version="1">MQKSIHFVPRGKHKGKGLAVFTSGGDSQGMNATLRAVVRMGLFIGCKVFLIKEGYEGMVAGGDYIVEAHWKSVSNILGEGGTAIGSARCMEFHQREGRLQAAYNLIIKEINNIVVIGGDGSLTGANLFRVEWASLLNELLENRKIVEEQREKFAFLKIVGLVGSIDNDFCGTDMTIGTDTALFRIVEAIDAIVSTAHSHQRTFILEVMGRHCGSPPPSDWPDKLCNKLIQERNAGQRLNIIIVAEGSIDRDGSPISAEDVKKVVVDKLRQDTRITVLGHIQRGGRPSAFDRALGMRMGAEAVLTLLETPPDEEPQVIALRGNHLTRVPLMDCVVRTKAVSQAMANKNWDLAVRLRGKSFERNLESFRKLSRLNPKNSELSIGFENEYPKGGFRFGVMHIGNPACGMNTAVRSFVKNLSYYSNVSVFAIRNGIDGLCSKLIEPISYKDVNGWTRMGGSNIGARQTLPEGKFMRIAEILREFNIHGLVIIGGFNAFHSAGQIFEQREKFPEFCIPIIVIPATCSNNLPGTELTLGLRLSAVGTNFRTFVVETQGGNSGYLSTLAGVAGGADKIYIPEENFSVRDLLDDVHHIKAKMRDGVQRAMVVISEFANANYNSEFLNRLYDEEGKDVFSCRKSIIGFVQQGQTPSPFDRRLAIKMGQMTADWILNQAKIALQLTGKVRTKECDSAVLLGLLKRQYVYSPLKSLIPESNFEKRCVKNASRLWWIKLGLLNKIIARHSSINDEEGIFVSLKSESLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKF9</accession>
    <name>T1GKF9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112264">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKF9"/>
    <dbReference type="STRING" id="36166.T1GKF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003982-RA">
        <property type="protein sequence ID" value="MESCA003982-PA"/>
        <property type="gene ID" value="MESCA003982"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048013_2_1_1"/>
    <dbReference type="OMA" id="NLAKECW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003982-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="20812" checksum="785EDE3E88024D14" modified="2013-10-16" version="1">MDRSATSIFGVRKYGIDINGYVRHPKLGLCLWQQQRSNTKETWPGKWDSLVSGGLSVGYGIFETAMKEAAEEASIPNNLLKNMISAGSVSFFFESENGLFPNTEYVFDLELPIDFIPQNSDGEVQAFELLPAEECLEKIFTSDFKTTSCPVVIDFLIRHGYITPENESNFTKIVELLHVPLQSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKR8</accession>
    <name>T1GKR8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Palmitoyl-protein thioesterase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKR8"/>
    <dbReference type="STRING" id="36166.T1GKR8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004095-RA">
        <property type="protein sequence ID" value="MESCA004095-PA"/>
        <property type="gene ID" value="MESCA004095"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375158_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098599">
        <property type="term" value="F:palmitoyl hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577463" description="Palmitoyl-protein thioesterase 1" evidence="1">
        <location>
            <begin position="25"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004095-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10373" checksum="85DB05127FDD24D7" modified="2013-10-16" version="1">MEIKTLSLIAIIILLLYYIQSQKAELTLTPVVLWHGMGDTCCLPFSLGHIATVIKENTAGSYVHSLKIGGNLIDDYKKTYPQPLTGLVGDPETSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKU7</accession>
    <name>T1GKU7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004128-RA">
        <property type="protein sequence ID" value="MESCA004128-PA"/>
        <property type="gene ID" value="MESCA004128"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1311395_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004128-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="24391" checksum="FB9BF54F7D5BCF97" modified="2013-10-16" version="1">MTVTKTKNSVNSFVLKAQASDHDHQLVVTSIQHVLVLLSAYSQQFLTRFVLVDHVGCWRTILDTILLLEEFRTSIPLQFYNFGGILRERKPSMKGFATNTSTCNTSYGDPITNKEEIVIQWIVSWICHWKNIIRSHRQVSGHRKSGEFSIKTKLVISSRKQKWDGWKTRYNHELNGGTLRRSFQHFVKKGHTVIFNGRKTSIEVWIEWGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKZ6</accession>
    <name>T1GKZ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKZ6"/>
    <dbReference type="STRING" id="36166.T1GKZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004179-RA">
        <property type="protein sequence ID" value="MESCA004179-PA"/>
        <property type="gene ID" value="MESCA004179"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877783_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15602" checksum="F5CEDAB63F745FC7" modified="2013-10-16" version="1">MIWITGDPQEDTSSSSETTPTSMSRKGKPSFSSRKTAKSVQNLPKRHVRSSSGYSSHNEETTFRKPTTVAHLIFFDSMSELRMNFYFVQQILQEFLRLPVHLFPNMILVFLSLPIFGTQMYEALHDLDVMFAFGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLA3</accession>
    <name>T1GLA3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02079317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLA3"/>
    <dbReference type="STRING" id="36166.T1GLA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004301-RA">
        <property type="protein sequence ID" value="MESCA004301-PA"/>
        <property type="gene ID" value="MESCA004301"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239671_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537:SF6">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="15"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004301-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11544" checksum="2B570B2792D73DC2" modified="2013-10-16" version="1">MTEYEIGIPIAGSPCSYAITPSMSINKTGALISPTYPGAYPKDMSCTYQFLGDINQRVRLEFRDFDLFFGGPHDEPRGRLRLVYKVSPGAAIVIENDIGVKNALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLM6</accession>
    <name>T1GLM6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004436-RA">
        <property type="protein sequence ID" value="MESCA004436-PA"/>
        <property type="gene ID" value="MESCA004436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984105_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14900" checksum="9502B27B1BDF388A" modified="2013-10-16" version="1">MYSPPFSYPRHSVVFHINVLNDNTSKNSQRHETYHKSQTQQISIINQPLRKVVQKEIKSSQKEKKLRLSNKTSHISFLFEMENRRKWFCFPLFFVAAAEKSFPLQFTIAIFPSKLNENNKAGKFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMA9</accession>
    <name>T1GMA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMA9"/>
    <dbReference type="STRING" id="36166.T1GMA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004678-RA">
        <property type="protein sequence ID" value="MESCA004678-PA"/>
        <property type="gene ID" value="MESCA004678"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898623_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004678-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15357" checksum="83EDBD36CC8F4079" modified="2013-10-16" version="1">MCEIPKSCRGRLCLSKIGLMREEQVWGAVGKLAELPCDLTPPIAQDSVKLLLWFKDTTGIPLYTTMHLPRGLPRPLPPATTAVLEVLKPPKTESQRHDEEYDRAIKKPRKKPIERIYREIPEGTKRDIKRNSYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMD1</accession>
    <name>T1GMD1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMD1"/>
    <dbReference type="STRING" id="36166.T1GMD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004702-RA">
        <property type="protein sequence ID" value="MESCA004702-PA"/>
        <property type="gene ID" value="MESCA004702"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984100_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001147">
        <property type="term" value="F:transcription termination site sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004702-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14717" checksum="A209579CC523067A" modified="2013-10-16" version="1">MNPEIFSWINNYFYGNKFKSVLNNDHCNNLVKSYVIFNVNDSYESKLAPGNYSNSKEADFIFGLLSKLFELMPYEMYSYGIVTPYLKQKSDLMNKIQTPNILDLIDSLQGKEKDIIYIQMLELVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMH4</accession>
    <name>T1GMH4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004748-RA">
        <property type="protein sequence ID" value="MESCA004748-PA"/>
        <property type="gene ID" value="MESCA004748"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3071069_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004748-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="53" mass="6224" checksum="77A70F728FC3BFA8" modified="2013-10-16" version="1">MKIKAINALNLANSPKIPLKNTVLEGNQHHHGDDDFELWDQSQMYRSENNQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMN7</accession>
    <name>T1GMN7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMN7"/>
    <dbReference type="STRING" id="36166.T1GMN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004815-RA">
        <property type="protein sequence ID" value="MESCA004815-PA"/>
        <property type="gene ID" value="MESCA004815"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_0_1"/>
    <dbReference type="OMA" id="AHYRNIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF65">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004815-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="20279" checksum="A9F23F1823E2F3D5" modified="2013-10-16" version="1">YGCDVNTKDADCRTTLYILALENKLDVVKYLLEVSNVDVNMPDSEGRTALHVAAWQGHVEMVKLLITLGNADVNSMDLECRTPLHSCAWQGNHDVMGILLYFGAVADHACKQGATALGISAQEGHEKCVSILLKYGANPYKSDHCGRTPIKLAAKSKGGGVGNVGDGKQANALIVLASKGNSCCLLKFCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN35</accession>
    <name>T1GN35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02053913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004974-RA">
        <property type="protein sequence ID" value="MESCA004974-PA"/>
        <property type="gene ID" value="MESCA004974"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852251_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004974-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7578" checksum="41122471531E56AA" modified="2013-10-16" version="1">MSPIYTLKIGTPNSKILLRIKWLELVKDDVAQNSLEKLCRRYKSEDMRSGQELWDNIKSIVGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNC4</accession>
    <name>T1GNC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNC4"/>
    <dbReference type="STRING" id="36166.T1GNC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005071-RA">
        <property type="protein sequence ID" value="MESCA005071-PA"/>
        <property type="gene ID" value="MESCA005071"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987155_0_0_1"/>
    <dbReference type="OMA" id="NRIMTTW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="44"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005071-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14491" checksum="418BEAC95ED70318" modified="2013-10-16" version="1">MKNPLFISTEKKATVLLGIKQFIYQIPEESTKTSILEMTAKVEALKNIFNKISFKQSIIFTNSQSRAESYKTYLVKEGWTSDVITGAMDQTDRLEIFKRFRTFQTKILVSTDLMSRGVDSEHLNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNM2</accession>
    <name>T1GNM2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005180-RA">
        <property type="protein sequence ID" value="MESCA005180-PA"/>
        <property type="gene ID" value="MESCA005180"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415768_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005180-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10794" checksum="DA7FD5CAAE33DD8A" modified="2013-10-16" version="1">MDQLCYDKFVVQLYICLPRQLGRSQQPIKAENTPISLKTKVKLYHQLITWFRKLESDIHRWEVVIGVRKKSLGLPTPLTHNITIYTSVEDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP65</accession>
    <name>T1GP65_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005382-RA">
        <property type="protein sequence ID" value="MESCA005382-PA"/>
        <property type="gene ID" value="MESCA005382"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_647761_0_0_1"/>
    <dbReference type="OMA" id="AGGTMQM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005382-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="424" mass="45613" checksum="0BEC0C3504BB7BFA" modified="2013-10-16" version="1">MMNPQGNGPSVNTANQLGGNINMAGPMGPGNQIPPNTAMNQNQSMMMQGGGPIGGMTAKYQQMIRAQAMQQQHMGPGPNGMGGSRPPPPEYKASQAQLMQAQMMHQGGGAGGRFANAAAMRRMAQQPIPPSGPMMRPQHSMYLQHSAGGGMGGPRSMGSYGAMGGMGPQRPPNVQVNPDGMPMGSQQEWRHMMMSQQQSMTFNVQSGGGVHMRQGGFNGGGYMPNAGNPNGNMPQGPNGMQMNPAQMQLMRQQQQQSVVGNQPNMMMANGGGGQQPPSVMQQMLQQSHHSSGQMQMTQMQMSSMQQSQQIQNQFGQQSVTTTSSHQQMIQMGGNGVPNTGPNASINQTINSVVANNSNDLSLEFLDSLPIGDAGNFSAQDLLNSLDNETLTFRTSYNKQFSCVEWFKYSRYFIFKLTSYSQNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ50</accession>
    <name>T1GQ50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02028920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005751-RA">
        <property type="protein sequence ID" value="MESCA005751-PA"/>
        <property type="gene ID" value="MESCA005751"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870188_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21010">
        <property type="entry name" value="AGAP001581-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21010:SF3">
        <property type="entry name" value="DAXX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005751-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7614" checksum="B24D634BDA6DFEE7" modified="2013-10-16" version="1">MSREEVIETNERLRALRIRLEESYDTAKRSLITLMNKYSDSKSNRNVFSRYNMLKIMIKVGSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ57</accession>
    <name>T1GQ57_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ57"/>
    <dbReference type="STRING" id="36166.T1GQ57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005758-RA">
        <property type="protein sequence ID" value="MESCA005758-PA"/>
        <property type="gene ID" value="MESCA005758"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840128_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005758-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16283" checksum="86DA379C1509EBF3" modified="2013-10-16" version="1">MRYVFQVNLTLREVEAQMQQMNTIEKDSGMSSPVGSSSSAIIRRRKHRASKSTTAWLTEAQTIHPKSHRHTIEKLMKLILEQGETIQQQLAKLRDRELQIARIEEERHKVREKELGKNYLLETYLNGLHEAEDRDVTTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRW3</accession>
    <name>T1GRW3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRW3"/>
    <dbReference type="STRING" id="36166.T1GRW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006407-RA">
        <property type="protein sequence ID" value="MESCA006407-PA"/>
        <property type="gene ID" value="MESCA006407"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202759_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7514" checksum="9485B4EAB0CF1EA1" modified="2013-10-16" version="1">MRQQPSLVDIHVPDGHKFTICGDIHGQFFDLMNIFKLNGIPSPTNPYLFNGDFVDRGSFSVEYLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS15</accession>
    <name>T1GS15_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with Ote.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02078859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GS15"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006462-RA">
        <property type="protein sequence ID" value="MESCA006462-PA"/>
        <property type="gene ID" value="MESCA006462"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000004">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000003">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="48"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="11">
        <location>
            <begin position="253"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="461" mass="51899" checksum="3DFCB52457F8E6BB" modified="2013-10-16" version="1">MPSEIITLQLGQCGNQIGFEFWKRLCLEHGISSSGVLEDFAADGLDRKDVFFYQADDDHYIPRAVLLDLEPRKIFICPSMVGVLEIIGLLVTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLCHSIAGGTGSGMGSYIMERLADRFPKKLIQTYSVFPNQDEISDVVVQPYNSMLTLKRLTTCADSVVVLDNTALNRIACDRLHLENPSFTQINTLVSTIMSVSTTTLRYPSYMNNNLIGLTAPLIPTPQLHFLMTGYTPLTTDNDQTVNVRKTTVLDVMRRLLQPKNMMVSTGHDKSNHHCYISILNIIQGEVDPSQVHKSLQRIRERKLAQFIPWGPTSIQVALSRSSPYVQSNHRVSGLMLANHTSICSLFERALTQYDKLKKRGAFLDQFKREDIFKDDLSELDESRNIVDCLVQEYEAATKPNYLQWSMGASRGEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS31</accession>
    <name>T1GS31_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006479-RA">
        <property type="protein sequence ID" value="MESCA006479-PA"/>
        <property type="gene ID" value="MESCA006479"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796876_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006479-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7860" checksum="36CDCAF77EAB886A" modified="2013-10-16" version="1">MLRNWGNFLLMILSPFWFSIIFAIIGFIMTFSSVIGDECQPNYEPCERAADCCSNHCDPYDRCMKSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS37</accession>
    <name>T1GS37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006485-RA">
        <property type="protein sequence ID" value="MESCA006485-PA"/>
        <property type="gene ID" value="MESCA006485"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431365_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006485-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21736" checksum="466B9DDB6F803E32" modified="2013-10-16" version="1">QILEKTQEHQVDTHHLTNIIFNKSNQKLGYADDNGLIGRQQQTFEQPSRVLKMKPSRIGLYVNGEKTKYILSSRNNANRNALGPNVNIEVVPNFICLGSKVTSDNNTSLFLNSHCPIIRDCHKKLVATLSLLRGEEENTYKRIQQTNRRCCNSNNYVHSVHLLIYGELYFNAVVSNLYLGNRDIMDDKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS77</accession>
    <name>T1GS77_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Megaselia</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="Aspartic_peptidase_AS"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASP_PROTEASE"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006526-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="318" mass="36004" checksum="6A8156827F191311" modified="2013-10-16" version="1">KNQGERNAAVVTQELTQNAVTTKSNPFRDNAPSKTVQQPQAARTYICYNCDEPGHTSRYCRKPYRPSCRECRRKGVYTNECGCRKEEAPKSICAKCRRVGYTTDSCPCNFPGSASMISRPQANSSVVHQVGAIQVKEDPRPSKIVMIGQYSFWAILDTGATTSYFAINTAVQESTGFSPAELNFGRQLRTANSLYEELAINVQKEAINPSDQVNKIKELCQVAKNNLEQASRNQARWYNLRHRDWSPEVNEIVYKKNHYLSNAANNFCAKLAPTYSGPYVVKNYISPTVVEVKEVENPRKVHRVHLKDLKQVDRSRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSA6</accession>
    <name>T1GSA6_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <authorList>
                <person name="Hughes D."/>
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        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA006558"/>
    </dbReference>
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    </dbReference>
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            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006558-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7961" checksum="69B0005E9FEBD0A6" modified="2013-10-16" version="1">MLIKRVTDHRFSRLKKGKGNTDHGNNGKRKNTLHSPLNQADVSFWEVFFFISPKWVACKDLEVMLGCRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSN5</accession>
    <name>T1GSN5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="1" key="2">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA006695"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3175940_0_0_1"/>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006695-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="47" mass="5700" checksum="00F5FF5C102CFB1F" modified="2013-10-16" version="1">MTGNPYSEIQLNLDWDCFQCCLMYSSSCNITFSIEMLKRLQFQRRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSU9</accession>
    <name>T1GSU9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="33"/>
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    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8133" checksum="3CF3A08C89CD36DA" modified="2013-10-16" version="1">MSRQDDELPIISDDEQDIQNRNNSNQRDNELERASGCGCCDPRSGVHRFIALIFMCLLGFEKRNQKTKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT18</accession>
    <name>T1GT18_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
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        <lineage>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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        </lineage>
    </organism>
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            <authorList>
                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175575">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT18"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006837-RA">
        <property type="protein sequence ID" value="MESCA006837-PA"/>
        <property type="gene ID" value="MESCA006837"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815378_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006837-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7864" checksum="59A712EA9D2C82D0" modified="2013-10-16" version="1">MINELSDSETVEAIGNAPSRNSKWKKWERLVFVSDIWIWRLQTFKKISSDVKLIDRDNIDEISANLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTT6</accession>
    <name>T1GTT6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007129-RA">
        <property type="protein sequence ID" value="MESCA007129-PA"/>
        <property type="gene ID" value="MESCA007129"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940520_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007129-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14970" checksum="14B846BA12963D91" modified="2013-10-16" version="1">MSISTTVRRFWDKVVVLVNYILQLTRGATRGGWNLCPSSKYLYETDIADAIEFAPIGLWCSFRDTTPIEQFYNNGKIAIQEFSSAFHNKEEETSPTRTIQRLKNNKSAGNVYNISIIEDDTDEVKNLFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTX3</accession>
    <name>T1GTX3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aconitase A/isopropylmalate dehydratase small subunit swivel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTX3"/>
    <dbReference type="STRING" id="36166.T1GTX3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007170-RA">
        <property type="protein sequence ID" value="MESCA007170-PA"/>
        <property type="gene ID" value="MESCA007170"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1717810_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="1">
        <location>
            <begin position="15"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007170-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="16984" checksum="7636ACD208338062" modified="2013-10-16" version="1">PFDKWDGKDLTDLTVLIKVKGKCTTDHISAAGPWLKYRGHLDNISNNMFIGATNIENNEMNKIKNQLTGEWAGVPDVARVYKSKGVKWVAVGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGLLPLTFANPADYDKVNLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU07</accession>
    <name>T1GU07_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393462">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007206-RA">
        <property type="protein sequence ID" value="MESCA007206-PA"/>
        <property type="gene ID" value="MESCA007206"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760696_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007206-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7844" checksum="E57710625D65A9DA" modified="2013-10-16" version="1">MTTSLENLNSRGTRIRGGISIKMSTICGTPICSASSDNLMDEHCEDLVNYLHQLCLCLRTQFCLSSHFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUF1</accession>
    <name>T1GUF1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IFT140 first beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02016227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUF1"/>
    <dbReference type="STRING" id="36166.T1GUF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007361-RA">
        <property type="protein sequence ID" value="MESCA007361-PA"/>
        <property type="gene ID" value="MESCA007361"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1046853_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000839">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056154">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF7">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 140 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23383">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT140 first beta-propeller" evidence="6">
        <location>
            <begin position="3"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007361-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="250" mass="28196" checksum="E0A5C5B17E512727" modified="2013-10-16" version="1">MTLYFDSKVQFIDDDAVSTIVKWHPVEPIFAVASYSSDRGGSVTIFDDSGEPLRDISYPVQSISQATALCWHPDKRILSTGWENGELYIWFNEHREFASVLGPHKAPIILMEFSEKGSRMVTADSMGLLTGWRCNGQYQFLTMFSHDLKDTLLHITFRRMVENQAKIELSNLTKAAVAGDESALDTLTSWRPKTASRNMVHSIVHDNHCFYVGTQSGCIFYLNQSGSCLEVYKSNNSSITQILWHPKGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUH2</accession>
    <name>T1GUH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007385-RA">
        <property type="protein sequence ID" value="MESCA007385-PA"/>
        <property type="gene ID" value="MESCA007385"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870183_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007385-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7116" checksum="F89DA78D147BCAC2" modified="2013-10-16" version="1">MEDIDEINALRKSATIIILLLKFCDCRFFETGNRRTSNQTGPRVADGGTLLCYGSKIDIVALTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUX4</accession>
    <name>T1GUX4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUX4"/>
    <dbReference type="STRING" id="36166.T1GUX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007546-RA">
        <property type="protein sequence ID" value="MESCA007546-PA"/>
        <property type="gene ID" value="MESCA007546"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111651_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00577">
        <property type="entry name" value="OPSINRH3RH4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007546-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="13232" checksum="2356393F41E0BF75" modified="2013-10-16" version="1">MMQICTNPVFTQPSKSPYKNASMLLEPLQFSNCRGSVNILRSGCDMTFLDDPYGNLTVLRPEARVGSSGSGERKFLGWNVPPEELQFIPEHWLTQYEPEKSFYKIYINFITYVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV22</accession>
    <name>T1GV22_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02140299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV22"/>
    <dbReference type="STRING" id="36166.T1GV22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007601-RA">
        <property type="protein sequence ID" value="MESCA007601-PA"/>
        <property type="gene ID" value="MESCA007601"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_2_1"/>
    <dbReference type="OMA" id="EWYVNIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF31">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="13"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007601-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="38929" checksum="3C43D91CF77F8C16" modified="2013-10-16" version="1">MTNKKSPEKLNPTVYMVFLSLLLDLLAFTMILPLFPSMLQYYKSNDSSGLYDYLSNYVKLFQNLVTGSPDSYNSVLFGGFLGSLFSFLQFVVSPIVGGFSDVYGRKPLMFLCTLGILSSYYLWTISTNFTVFIIARIVGGLSKGNISLYMAVITDLTNVQNRGRGMALVGIAFSIGFIVGPMIGAMFSKFVIKHPYCAICLTLANLLVIGFGIKESLPSEKRIAKFKSSMSYAVKFLNISSIFSFSIVNKLKAHEKPTLQRIGFIYFVYLFVYSGLEFTASFLMFHKFGFNSIDQAKMFLTTATSFVVTCMTTMVSKFGNVDEKGTILGVFRSLGALARALGPVFGSIVSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB3</accession>
    <name>T1GVB3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007704-RA">
        <property type="protein sequence ID" value="MESCA007704-PA"/>
        <property type="gene ID" value="MESCA007704"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657296_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588556" description="Secreted protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007704-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8933" checksum="03540D490587418A" modified="2013-10-16" version="1">MRNFTLRWSVSTWLIFFFMAPSGEAQVFLWILGCGKNVHYKAHTVACCWLSYSSYNVKHARLKKLYPDISPQVFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVV0</accession>
    <name>T1GVV0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02119498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007917-RA">
        <property type="protein sequence ID" value="MESCA007917-PA"/>
        <property type="gene ID" value="MESCA007917"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760713_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007917-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7977" checksum="32226D2A5BCE0A7D" modified="2013-10-16" version="1">MSSIISKNSTGCQFLYFYKDLLAFIMNTEIGTYTIHPKGRNFTDSSLKLLDNILPQLDLPDTCSIWCLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW51</accession>
    <name>T1GW51_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008022-RA">
        <property type="protein sequence ID" value="MESCA008022-PA"/>
        <property type="gene ID" value="MESCA008022"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673912_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008022-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="9220" checksum="BF78BF96EE315151" modified="2013-10-16" version="1">MSFHLQLFKSLMKETLLGIDVDLSGIKGRKPSHLRHIWKLEFVQRLWLNSSICRKEDLYEFLEWFRIRDKIWKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWB4</accession>
    <name>T1GWB4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWB4"/>
    <dbReference type="STRING" id="36166.T1GWB4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008087-RA">
        <property type="protein sequence ID" value="MESCA008087-PA"/>
        <property type="gene ID" value="MESCA008087"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940519_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008087-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="15150" checksum="402016B6E64DD84C" modified="2013-10-16" version="1">MTPEDFQKNLRGLNGNNDFDPEMLLEIYNSIKNEEIIMPAEQTGLVKENFLWKVLLRRGLTTEGIYHHIPDATFDKEVFKVIWGPSLAALSFMNDKSTEIGYQRTLKDSKWRTFCKSRCCFPLSICYNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWC9</accession>
    <name>T1GWC9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171248">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008102-RA">
        <property type="protein sequence ID" value="MESCA008102-PA"/>
        <property type="gene ID" value="MESCA008102"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867444_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008102-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15830" checksum="A856CD5D43AE7573" modified="2013-10-16" version="1">MGFFDSSHGNAKWPLDYSCHIRNIDGQSEVFNSSIKIIQNCNIEIPSSAVRRPKTRMFIKRYWSFPITTFALSTFERTNKRLKKRNKVSFPLHDGNEEKNHRGTPENLSDFVLFMFSCLCLRGPGMDIVGNLRNLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWS9</accession>
    <name>T1GWS9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WDR5-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008259-RA">
        <property type="protein sequence ID" value="MESCA008259-PA"/>
        <property type="gene ID" value="MESCA008259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_1_1"/>
    <dbReference type="OMA" id="CKGHDTA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000228">
        <property type="entry name" value="COMPASS-like H3K4 histone methylase component WDR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="5">
        <location>
            <begin position="131"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="214"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="296" mass="32748" checksum="D6BDD1D2AFA6ABF1" modified="2013-10-16" version="1">ADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSRLLVSASDDKTLKIWELSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDEPPPLPGQNALSQQSSNRSSSNAQRPNYTLKFTLAGHTKAVSASQWXDPISAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKCKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQSKEVVQRLQGHTDTVLTTACHPTENIIASGALENDKTIKLWKSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWW3</accession>
    <name>T1GWW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196921">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008297-RA">
        <property type="protein sequence ID" value="MESCA008297-PA"/>
        <property type="gene ID" value="MESCA008297"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1733588_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="17533" checksum="79A9D1E878B90FD8" modified="2013-10-16" version="1">MFWNFIQAQGWALSVIYKRNLRVNYTHGLSYVDFEPGDKRVRGSGRTTKYRDKSDNETESLPIKSLDKIEQICLRQHEETNIKIHGTWFGWGRSTQSTQSRKQLDFEVFKSDQNSAPGSKMRIRKWFVCLKSGQNSVTGPKKRNSGKLDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXU6</accession>
    <name>T1GXU6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02099810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXU6"/>
    <dbReference type="STRING" id="36166.T1GXU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008655-RA">
        <property type="protein sequence ID" value="MESCA008655-PA"/>
        <property type="gene ID" value="MESCA008655"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2229852_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF5">
        <property type="entry name" value="FI02094P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008655-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12278" checksum="25024CE927E96279" modified="2013-10-16" version="1">MDPNLLPSFMRKFTTSKEYLSVRPDIKERSRIIHAFEIKENLSNKINQYFDLRFGRSLRLFGSVMFFGNMITWLPITAYVPALTFNQVTGINVHVITPVVCAICTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYK1</accession>
    <name>T1GYK1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYK1"/>
    <dbReference type="STRING" id="36166.T1GYK1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008931-RA">
        <property type="protein sequence ID" value="MESCA008931-PA"/>
        <property type="gene ID" value="MESCA008931"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654141_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008931-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="16687" checksum="2743A059D4621466" modified="2013-10-16" version="1">MGAKASVPTSATTSAGGGQNPRTRTYSSSTEGGIVGGGVGSAGSGSGSNSNSSSGASATGFNLLRVLPSGLQQHTTPNQSQSDRQRARSLSSVPDLHNQQNNHLHHNQQNNNHNHHNHLHHQQSHNNHHHEDNNGGQSHSAGASGMLGLGGRMALRQHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYS1</accession>
    <name>T1GYS1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sterol carrier protein 2</fullName>
            <ecNumber evidence="15">2.3.1.155</ecNumber>
            <ecNumber evidence="16">2.3.1.16</ecNumber>
            <ecNumber evidence="4">2.3.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Acetyl-CoA C-myristoyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Non-specific lipid-transfer protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Propanoyl-CoA C-acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">SCP-2/3-oxoacyl-CoA thiolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">SCP-2/thiolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">SCP-chi</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Sterol carrier protein X</fullName>
        </alternativeName>
    </protein>
    <organism evidence="42 43">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="43" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="43">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="42" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="28">Mediates the transfer of all common phospholipids, cholesterol and gangliosides from the endoplasmic reticulum to the plasma membrane. May play a role in regulating steroidogenesis. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol. Also binds fatty acids and fatty acyl Coenzyme A (CoA) such as phytanoyl-CoA. Involved in the regulation phospholipid synthesis in endoplasmic reticulum enhancing the incorporation of exogenous fatty acid into glycerides. Seems to stimulate the rate-limiting step in phosphatidic acid formation mediated by GPAT3. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs.</text>
    </comment>
    <comment type="function">
        <text evidence="29">Plays a crucial role in the peroxisomal oxidation of branched-chain fatty acids. Catalyzes the last step of the peroxisomal beta-oxidation of branched chain fatty acids and the side chain of the bile acid intermediates di- and trihydroxycoprostanic acids (DHCA and THCA). Also active with medium and long straight chain 3-oxoacyl-CoAs. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol and transfers phosphatidylcholine and 7-dehydrocholesterol between membrances, in vitro. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>3-oxo-(9Z-octadecenoyl)-CoA + CoA = (7Z)-hexadecenoyl-CoA + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:47400"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:87695"/>
            <dbReference type="ChEBI" id="CHEBI:87698"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:47401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>3-oxohexadecanedioyl-CoA + CoA = tetradecanedioyl-CoA + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:40343"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:77081"/>
            <dbReference type="ChEBI" id="CHEBI:77084"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:40344"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>7-dehydrocholesterol(in) = 7-dehydrocholesterol(out)</text>
            <dbReference type="Rhea" id="RHEA:62960"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="34">
            <dbReference type="Rhea" id="RHEA:21566"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>butanoyl-CoA + acetyl-CoA = 3-oxohexanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:31111"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:62418"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="33">
            <dbReference type="Rhea" id="RHEA:31113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>choloyl-CoA + propanoyl-CoA = 3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholestan-26-oyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:16865"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57373"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58507"/>
            <dbReference type="EC" id="2.3.1.176"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:16867"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>decanoyl-CoA + acetyl-CoA = 3-oxododecanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:31183"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
            <dbReference type="ChEBI" id="CHEBI:62615"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="32">
            <dbReference type="Rhea" id="RHEA:31185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>dodecanoyl-CoA + acetyl-CoA = 3-oxotetradecanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:31091"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="36">
            <dbReference type="Rhea" id="RHEA:31093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>hexadecanoyl-CoA + acetyl-CoA = 3-oxooctadecanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:35279"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:71407"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="35">
            <dbReference type="Rhea" id="RHEA:35281"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>hexanoyl-CoA + acetyl-CoA = 3-oxooctanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:31203"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:62619"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="31">
            <dbReference type="Rhea" id="RHEA:31205"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>octanoyl-CoA + acetyl-CoA = 3-oxodecanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:31087"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="38">
            <dbReference type="Rhea" id="RHEA:31089"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>propanoyl-CoA + tetradecanoyl-CoA = 3-oxo-2-methylhexadecanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:46344"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:86042"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:46346"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>tetradecanoyl-CoA + acetyl-CoA = 3-oxohexadecanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:18161"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="EC" id="2.3.1.155"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="30">
            <dbReference type="Rhea" id="RHEA:18163"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.155" evidence="15"/>
    <dbReference type="EC" id="2.3.1.16" evidence="16"/>
    <dbReference type="EC" id="2.3.1.176" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02386065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYS1"/>
    <dbReference type="STRING" id="36166.T1GYS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009008-RA">
        <property type="protein sequence ID" value="MESCA009008-PA"/>
        <property type="gene ID" value="MESCA009008"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_0_1_1"/>
    <dbReference type="OMA" id="PSLYAMM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050633">
        <property type="term" value="F:acetyl-CoA C-myristoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000016">
        <property type="entry name" value="Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000001">
        <property type="entry name" value="Putative Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006102">
        <property type="entry name" value="PRK08256.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="43" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="39">
        <location>
            <begin position="2"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="41">
        <location>
            <begin position="236"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="SCP2" evidence="40">
        <location>
            <begin position="395"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00030851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00031275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00032093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00032316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00033178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00045738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00045994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00047485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00049268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00049270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00049306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00049542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="41">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009008-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="43">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="523" mass="57003" checksum="4E707C050A2351A3" modified="2013-10-16" version="1">MAKEAVTKALKDAKIDFTEIKQAVVGYVYGDSTSGQRALYEIGMTGIPCYNVNNNCSTGSSALYLAKQLIDTGDNDCVLALGFEKMEKGSLSSKYFDRANPMEKHIETMSELADIGAGPMTAQMFGNAGEEHMKKYGTKPEHFAKIAWKNHKHSLLPIQNEYSLEQVMKSPQIHGVLTKLQCCPTSDGSAACIVANEQFVRRHNLESQAVEIVGMEMATDLESTFKGESLMKVVGYDMTKLAADRLFAKSQYKPTDVNVVELHDCFSANELITYEALGLCPPGKAGELIDNGDNTYGGKYVINPSGGLISKGHPLGATGLAQCSELCWQLRGEADKRQVPNCKLALQHNIGLGGAVVVGLYRLGFPKMAKNLTAAVNEIADSPEGFKVTPMMALLEQAMADDTDNLIEKVRAIYGFKVTNGPKGKTGYWVINAKQGKGKVIYNGKDKPDITFIVNDEDVTDLIAGKIPPQKAFFQGKVKIQGNMGLAMKLIDLQRSAASRLKLLKLSCGVVNKRYFLLFKCGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZG7</accession>
    <name>T1GZG7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02386742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZG7"/>
    <dbReference type="STRING" id="36166.T1GZG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009267-RA">
        <property type="protein sequence ID" value="MESCA009267-PA"/>
        <property type="gene ID" value="MESCA009267"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_6_2_1"/>
    <dbReference type="OMA" id="AGMSNTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009267-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="261" mass="28215" checksum="B1F2CE96F53721D7" modified="2013-10-16" version="1">MQRMAHPEGEVANAKACGKMGSIFMLSTLATSSLQELEAQAPETVKWFQLYIYKDRSLTETLVRTAEKCGFKAIVLTIDAPVFGIRRKDVRNKFSLPPGLKLANFEGIQAKGVKSSGASGINEYVASQFDDTLTWDDVKWLVNFTTLPVVAKGVLTKEDAILALHNGCKGVIVSNHGARQVDGVPATIEVLPEIVKAVGNQMVVMLDGGVSQGVNVFRALALGAKYVFMGRPAIWGLAVNGQTGVEHVLEIVRSELENTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZI1</accession>
    <name>T1GZI1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZI1"/>
    <dbReference type="STRING" id="36166.T1GZI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009282-RA">
        <property type="protein sequence ID" value="MESCA009282-PA"/>
        <property type="gene ID" value="MESCA009282"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545185_0_0_1"/>
    <dbReference type="OMA" id="MWRVNHQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009282-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9973" checksum="E40FEF9F8845AFFE" modified="2013-10-16" version="1">MWRVNHQSLIPVTRKIDSDSRYMSTEEQNRELTRSIRREVAKLSEQWNNLIDKSDNWKQRLDDFMTVSGKWYTIVIGLPCTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZJ9</accession>
    <name>T1GZJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a molecular chaperone for G protein-coupled receptors, regulating their biogenesis and exit from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZJ9"/>
    <dbReference type="STRING" id="36166.T1GZJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009302-RA">
        <property type="protein sequence ID" value="MESCA009302-PA"/>
        <property type="gene ID" value="MESCA009302"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026137_1_0_1"/>
    <dbReference type="OMA" id="YPMHQSV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000302">
        <property type="entry name" value="Ankyrin repeat containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021832">
        <property type="entry name" value="ANKRD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055285">
        <property type="entry name" value="ANKRD13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447:SF25">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11904">
        <property type="entry name" value="ANKRD13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Ankyrin repeat" evidence="10">
        <location>
            <begin position="182"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="318"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="318"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009302-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="477" mass="54634" checksum="3EEE503A72CE828F" modified="2013-10-16" version="1">MSVNGDQDSEDGYASAEEELPDQQKPQQKIDHEEEYPLHRSVFLDDKKTLSQLLRQPEKYDISQKDKHGNTALHLSVMLGRKALTHLLLAHHAPVKIKNSDGWSVLSEAISYGDRQTITSVLRALKAQTRMQMQERRGSLIEALNQIGDFYMELNGTFIHGCLSRILPSDVCKIHKCGSKIRLDTTLVDFTDMRWERGDISFIFRGDKNPTESLTVLDNEYKCFQRIRHEESENEIEDEVDVLMSSDILAAQMSTKKINFSRAQTGWFFRADRKELVAGQYNSDLFTINGLVLKSRKRREHLSSDDLQKNKMLVESFTKGSNSSSGSLTPNGSLKNEPYEVSRRSSLIPPPETNVTWEDYINAEVGEMPSIGRKMVYKESSKHFKATVSMSTDFPLSIEMLLNVLEVIAPFKQFTKLREFITLKLPSGFPVKIEIPILRTVSAKITFQRFDFQDNISEELFEVPSDYVEDSRRFPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H059</accession>
    <name>T1H059_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H059"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009527-RA">
        <property type="protein sequence ID" value="MESCA009527-PA"/>
        <property type="gene ID" value="MESCA009527"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742954_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009527-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7862" checksum="112558740E8ADB56" modified="2013-10-16" version="1">MEFFLSLKALNVEPYNVGFIIFGNDKVIKEGDIALDNAIDAIINDGQDENKNQTNAMKSCWCNEIGIGQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H076</accession>
    <name>T1H076_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H076"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009547-RA">
        <property type="protein sequence ID" value="MESCA009547-PA"/>
        <property type="gene ID" value="MESCA009547"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3144420_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009547-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="49" mass="5582" checksum="2ED5FCBBBADA32D1" modified="2013-10-16" version="1">MSCCCAFTLLAKAVLVCQLIFKVFPWLYQNVAPKFRDPKSKSRNSENGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0J5</accession>
    <name>T1H0J5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379592">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0J5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009674-RA">
        <property type="protein sequence ID" value="MESCA009674-PA"/>
        <property type="gene ID" value="MESCA009674"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815345_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009674-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7619" checksum="9C2F42A189CFAE1F" modified="2013-10-16" version="1">MKLVYVNNYFNIEKSFGFTVIKPLDLNNHGGNLALNILNMGIISTSAKEKYYLSDEGNWKKCCPEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0T2</accession>
    <name>T1H0T2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the Mettl1-dependent formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the Mettl1-wuho methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit. Required for binding of nanos mRNA and repression of translation by the mei-P26-bgcn-bam-sxl complex. May cooperate with mei-P26 and nanos to derepress the BMP signaling pathway. May cooperate with mei-P26 to suppress expression of a subset of microRNAs. May cooperate with mei-P26 to regulate bam expression levels in germline cells during gametogenesis. Required to promote mitosis to meiosis transition during gametogenesis. May regulate germline cell division in part by regulating ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a heterodimer with the catalytic subunit Mettl1. Interacts with mei-P26 and weakly interacts with bgcn; required for the function or formation of the mei-P26-bgcn-bam-sxl complex. Interacts with nanos; may be involved in mei-P26-dependent derepression of the BMP signaling pathway. Interacts with Myc; the interaction may be mediated by mei-P26 and may be involved in the regulation of ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0T2"/>
    <dbReference type="STRING" id="36166.T1H0T2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009772-RA">
        <property type="protein sequence ID" value="MESCA009772-PA"/>
        <property type="gene ID" value="MESCA009772"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412094_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036265">
        <property type="term" value="P:RNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028884">
        <property type="entry name" value="Trm82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288:SF0">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288">
        <property type="entry name" value="WD40 REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009772-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="21570" checksum="6D1841BE5C5AB079" modified="2013-10-16" version="1">MTHMEHELVIALNRKIIFVNPNDLQIFKEVEIESDLVPLGLKLSKDDNDDESKTKKPKSQEEAKIQHVALSDDLQWLAVTTSGQKALLLFKCLPVHAKLVSIRPIPRASSALIFSPSGSSVLVCDKTGDCYQFDVSEDSQNPGKLVLGHLSIVYDVLFPPGENHIITSDRDEKIRVTNYPNSYEIANYCLGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1J0</accession>
    <name>T1H1J0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02378501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1J0"/>
    <dbReference type="STRING" id="36166.T1H1J0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010052-RA">
        <property type="protein sequence ID" value="MESCA010052-PA"/>
        <property type="gene ID" value="MESCA010052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="OMA" id="QQYKETD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="53026" checksum="E5A2DF4743AC4549" modified="2013-10-16" version="1">MGLIMPILLIISIFILVITYLSWNYNYWKKRGVNGPRPVLVFGNFPKTGRGEAHMIYEIDKYYQSSEKFIGLFWARTPQLLLLDPKLIKDILITNFKAFHDNDSSHWPIKNVDRMAVSNPFMVAGDAWKDKRIEVVSGITSAKLKTSYPIMKNAATKLSNYLKENLNKTSELEAKNFSQKFTGEIIGDFLWGIEAGAFTSPDDDCPIVLSAQDTDKFFLNIQKEAMAMRLNSKHKRGDMLDHLLQIQEKKHISTVDITGHSLTVVLDGFESSGLILSQTLLFLSRNIGAQQKLRQEIMAHLDDSGFLSFEDLNDLPYLDQCVNESLRILPAVPILGKICTEPFEFENSNRKRVKINKGLISLIPIYSLHHDPDVYPDSESFIPERFDEENGGVKKYKESGHFMPFGDGPRICLGMKLTLVEIKTGVVEIIKNFELTCSDKTKPGNEANDDTAYLLSFKGDIVLNMKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1W7</accession>
    <name>T1H1W7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1W7"/>
    <dbReference type="STRING" id="36166.T1H1W7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010190-RA">
        <property type="protein sequence ID" value="MESCA010190-PA"/>
        <property type="gene ID" value="MESCA010190"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_4_4_1"/>
    <dbReference type="OMA" id="THEYRCP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06168">
        <property type="entry name" value="LSMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034110">
        <property type="entry name" value="LSMD1_Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF5">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 38, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="18"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010190-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10698" checksum="F2BB2821BEBB8774" modified="2013-10-16" version="1">MENDPILKPAPPAEALSECRENLRKWLGKPFRVVITDGRILQGIFNCTDKDANIVLTMSDEYLEEGQEARHLGSVMIPGKHIVSVHVDSSMLVNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2A4</accession>
    <name>T1H2A4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2A4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010341-RA">
        <property type="protein sequence ID" value="MESCA010341-PA"/>
        <property type="gene ID" value="MESCA010341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725070_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8367" checksum="33D64665AB5C62CB" modified="2013-10-16" version="1">MTKSLRLRIAIGTFGYGTLKLELKSDGLFKKTDMMFWNNFEENIVTNLKKENPVKVELQLRLLEALEKDVCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2E2</accession>
    <name>T1H2E2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187188">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2E2"/>
    <dbReference type="STRING" id="36166.T1H2E2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010385-RA">
        <property type="protein sequence ID" value="MESCA010385-PA"/>
        <property type="gene ID" value="MESCA010385"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294856_0_0_1"/>
    <dbReference type="OMA" id="QLTIATM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043083">
        <property type="term" value="C:synaptic cleft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001507">
        <property type="term" value="P:acetylcholine catabolic process in synaptic cleft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001445">
        <property type="entry name" value="Acylcholinesterase_insect"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF13">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00880">
        <property type="entry name" value="ACHEINSECT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010385-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11277" checksum="77EFF61FE0647905" modified="2013-10-16" version="1">MDRLVVQTSSGPVRGRVVNVQGREVHVYTGIPYAKPPVDELRFRKPVPAEPWHGVLDATRQPSTCVQEKHIERLILVNVICSSALFGKSRRNRPIGLAILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3H5</accession>
    <name>T1H3H5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3H5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010803-RA">
        <property type="protein sequence ID" value="MESCA010803-PA"/>
        <property type="gene ID" value="MESCA010803"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888131_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010803-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="14945" checksum="CEAEC95B08539E0E" modified="2013-10-16" version="1">MISTKIVGDGKQSNTSIVLAIASITFLWTGHIMPSFKAAGAEKIWGDAIEEVIFGKNGCWYLLVVSWIHMVIAKASISVALRELRKLAPVSMSSIIIGSGCQILIFLKDYGNWHLRCHFLASRWLLLPRSRSASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H455</accession>
    <name>T1H455_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121040">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H455"/>
    <dbReference type="STRING" id="36166.T1H455"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011056-RA">
        <property type="protein sequence ID" value="MESCA011056-PA"/>
        <property type="gene ID" value="MESCA011056"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309192_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011056-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11578" checksum="4C4C7B951E4ED93C" modified="2013-10-16" version="1">MKSNELSTNIGSLLNNCNNLSPIRDEQQIQEHHINNTTNANWNSDSWADGEFEPIEEPLTGNPKLDEARRKREEKKLQRQRELEARRAQKGPMKLGARKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4G5</accession>
    <name>T1H4G5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4G5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011174-RA">
        <property type="protein sequence ID" value="MESCA011174-PA"/>
        <property type="gene ID" value="MESCA011174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2405686_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="44"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10453" checksum="FF72C43D4D9ABC01" modified="2013-10-16" version="1">MAHCMFTFTTDTFQVTNHNYLILAMKLILAAFLFLGLSGNQVLGQNCNGKTRGYYVTPNSCNQYYYCDGGKIWTYNCATAPSGWAKDFFCTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H573</accession>
    <name>T1H573_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium load-activated calcium channel</fullName>
            <shortName evidence="14">CLAC channel</shortName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Calcium-selective channel required to prevent calcium stores from overfilling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TMCO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02149657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H573"/>
    <dbReference type="STRING" id="36166.T1H573"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011448-RA">
        <property type="protein sequence ID" value="MESCA011448-PA"/>
        <property type="gene ID" value="MESCA011448"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081121_0_0_1"/>
    <dbReference type="OMA" id="GMFGDFK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008559">
        <property type="entry name" value="TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917:SF0">
        <property type="entry name" value="CALCIUM LOAD-ACTIVATED CALCIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917">
        <property type="entry name" value="PNAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023322">
        <property type="entry name" value="DUF841_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 14" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011448-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="20856" checksum="E78E0DD81F100964" modified="2013-10-16" version="1">MWSDTLLILFISVCTALLGEGLTYVLVYRTEKYIKLKNEVEKASKKLEKRKEHQNQNDKSQKKKIERDEEKLKNNNRDLSLVKMKSMFATGFAFTALLSMFNTIFDGKVVARLPFTPISWIQGLSHRNLSGEDYTECSFIFLYILCTMSIRQNIQKLLGFAPSRAASKQGNSLFAPAPGQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5A8</accession>
    <name>T1H5A8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5A8"/>
    <dbReference type="STRING" id="36166.T1H5A8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011485-RA">
        <property type="protein sequence ID" value="MESCA011485-PA"/>
        <property type="gene ID" value="MESCA011485"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1125644_0_0_1"/>
    <dbReference type="OMA" id="ECNICQF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF67">
        <property type="entry name" value="FI01116P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011485-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="28190" checksum="65ACCB3CA46FA414" modified="2013-10-16" version="1">MKVHKNWRSKVVRPIKKQKPPKPTTSKEFAVPQTKYYSEKTCEICERKFVNGKTLSKHVKSVHLKIKPFICNVCGFKCSRKSVLEVHSRQHKKEKPLECNICQFKTRDQSSLQTHKKRHLSHATQNHKCFVCDYTAIQTSNLKTHIRTKHPENFKDISCDLCSFVTVSKEKLAYHKNDHTKGLVLKGSNDDDDKKNESNGIQNKTLGANEISSDCFLPMESTDSINHEPALDTGGVTIPAHSEDAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5M3</accession>
    <name>T1H5M3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5M3"/>
    <dbReference type="STRING" id="36166.T1H5M3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012006-RA">
        <property type="protein sequence ID" value="MESCA012006-PA"/>
        <property type="gene ID" value="MESCA012006"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_8_1"/>
    <dbReference type="OMA" id="TIYEMEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012006-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8438" checksum="7DCE20533F524477" modified="2013-10-16" version="1">EEALKLANDTRRGLAGYFFSEDMRQVFRVAKKLEVGMIGVNEGLISTAEAPFGGVKESGIGREGSHQGIDEYVDVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5U5</accession>
    <name>T1H5U5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5U5"/>
    <dbReference type="STRING" id="36166.T1H5U5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012078-RA">
        <property type="protein sequence ID" value="MESCA012078-PA"/>
        <property type="gene ID" value="MESCA012078"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631257_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="87"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012078-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18671" checksum="763BE6D3E2C47559" modified="2013-10-16" version="1">MVVNSNGPSNDTNSWETQVFAPPEDLIATSQLEFELPGLEPKITLVLRDLGTQPSSQIYTVKTPPERTITPPTISDYRPDFQDIMKNVEDPELNASQTNSTWLRLGWKKMSDEQMEYVDGLQIRYKELTAMLYDATPLIHRSLNSYTIENLKPDTGYEFGLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H609</accession>
    <name>T1H609_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H609"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012142-RA">
        <property type="protein sequence ID" value="MESCA012142-PA"/>
        <property type="gene ID" value="MESCA012142"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_10_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008089">
        <property type="term" value="P:anterograde axonal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732:SF7">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012142-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21890" checksum="09C0EE1B19358A58" modified="2013-10-16" version="1">MLALINRILDWFKSLFWKEEMELTLVGLQFSGKTTFVNVIASGNFTEDMIPTVGFNMRRITKGNVTIKVWDIGGQPRFRSMWERYCRGVNAIVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXREICCYSISCKEKDNIDITLQWLINHSKSSHIIYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H655</accession>
    <name>T1H655_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear respiratory factor 1 NLS/DNA-binding dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRF1/Ewg family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H655"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012188-RA">
        <property type="protein sequence ID" value="MESCA012188-PA"/>
        <property type="gene ID" value="MESCA012188"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617640_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039142">
        <property type="entry name" value="NRF1/Ewg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019525">
        <property type="entry name" value="Nrf1_NLS/DNA-bd_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20338">
        <property type="entry name" value="NUCLEAR RESPIRATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10491">
        <property type="entry name" value="Nrf1_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Nuclear respiratory factor 1 NLS/DNA-binding dimerisation" evidence="7">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="444" mass="48900" checksum="EF6C49FC9A38B892" modified="2013-10-16" version="1">QAIVLVATPGKPNTSFKVFGAKPLEDVVRNLKSVVMEELETALANQAPPPQQEDPTLFELPPLIIDGIPTPVEKMTQAQLRAFIPLMLKYSTGRGKPGWGRESTRPPWWPKELPWANVRMDARTEEDKQKISLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLHQGEGGATIQTVQSLTDANGHENMTVDLTEATLGQDGQIIITGEDGQGYPVSVGNMITLPVSSSIYQTMMANIQHIQPNGDGTVCIAPMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H718</accession>
    <name>T1H718_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 185A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H718"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012500-RA">
        <property type="protein sequence ID" value="MESCA012500-PA"/>
        <property type="gene ID" value="MESCA012500"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053027_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019396">
        <property type="entry name" value="TM_Fragile-X-F-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568">
        <property type="entry name" value="FAM11A, B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10269">
        <property type="entry name" value="Tmemb_185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012500-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29859" checksum="08E427D1CA98A5A8" modified="2013-10-16" version="1">VGADSYIQFKAMLVSISLHLILLMFEILACDNLSSGGRHHWIIVFVPLILGSIASIKACVWAVKHDRSFELELFLAVNALQFISLPLKLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLLLAILSLIVLSFCAKGGNKWWFGIRKNFYQFILSFLPFLQEYGNISYRPNKCQFRKWPKCTIDFEEESEERREKIECNKPVVAIHSIYSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-11-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H738</accession>
    <name>T1H738_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H738"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012520-RA">
        <property type="protein sequence ID" value="MESCA012520-PA"/>
        <property type="gene ID" value="MESCA012520"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645112_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012520-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8842" checksum="5562CF504C05C414" modified="2013-10-16" version="1">XQTILCILLERLLYTEFITVVCNEHQITLLRVDSYKKLSESSSNIHKEKPSKPSCSLFVIKDFPAVDVVKVHLRNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H741</accession>
    <name>T1H741_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H741"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012523-RA">
        <property type="protein sequence ID" value="MESCA012523-PA"/>
        <property type="gene ID" value="MESCA012523"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874317_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01465">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="1">
        <location>
            <begin position="15"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012523-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7568" checksum="FC16FB1FD6638DB7" modified="2013-10-16" version="1">TIYRRLLEAQKNKQHVDPEVTLQFLKSAIYYFLTDKENSQGHLKAIESILEFTEQEKNNISKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H772</accession>
    <name>T1H772_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H772"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012610-RA">
        <property type="protein sequence ID" value="MESCA012610-PA"/>
        <property type="gene ID" value="MESCA012610"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_574387_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052267">
        <property type="entry name" value="N-DRC_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690:SF0">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012610-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="53388" checksum="8E58BC6B69B8555B" modified="2013-10-16" version="1">FPSEENGGSLHIFSRPGTESEMSRSSAISSKESRKTKDKSKSPARSADLNVEDNLEEGFRPGQSVVLPDMFNDVWKNKDAGLVKQSYYEDMVYNEKYAEVERELRKIVDDLMRQELELLQSAIGKKIKKSNKKARRSGKKNKKKKEKDLTPDRTPESLYEELVTNGIIRKYPDIWLKDFCGDKSYAERSGTNPSPGDIRQLIKEYIILPLGSDVVRNSCPVIRSILFAGSKGSGKRTLLHAICNEVGATLFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H796</accession>
    <name>T1H796_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H796"/>
    <dbReference type="STRING" id="36166.T1H796"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012634-RA">
        <property type="protein sequence ID" value="MESCA012634-PA"/>
        <property type="gene ID" value="MESCA012634"/>
    </dbReference>
    <dbReference type="OMA" id="DNYEANS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF2">
        <property type="entry name" value="SPLICING FACTOR YJU2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012634-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19448" checksum="BA29CBE3AC9FAD9D" modified="2013-10-16" version="1">WDPLQNEQIVPETKETQKKLFDDPMYKLEHQSKDVQAADDAKPAIEKLYLRNSDVWKDNYEANSLLRAQFRKTKKDLKAKEDLDKKLLMKSSLSIELLPENDQDRQMASLMTLQSRSAKEREEEKRLDLLIKPALPSSTMTSFGGLKRQKLLSSKLSVEELGIKKKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7C3</accession>
    <name>T1H7C3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7C3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012661-RA">
        <property type="protein sequence ID" value="MESCA012661-PA"/>
        <property type="gene ID" value="MESCA012661"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_895950_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="311" mass="33802" checksum="73AB55358AA630C6" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASCNGDTWASELGTVLGTGNPFLITTLKRVPKGTNGGISIIGLIVSFLGGTLVGLAYFLTILYTDDNRLLNPPQWPIILIGGFSGLFGSVLDSFLGATFQYSGLDTDGKIADRPGKQTRHISGLSILDNHSVNLISCFLTAVTIPIIAVKVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAC4</accession>
    <name>T1GAC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAC4"/>
    <dbReference type="STRING" id="36166.T1GAC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000179-RA">
        <property type="protein sequence ID" value="MESCA000179-PA"/>
        <property type="gene ID" value="MESCA000179"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026186_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="18417" checksum="FBA5FD31F0824075" modified="2013-10-16" version="1">MAQSDLESIFSPYGKIITSRILSDNVSDGSKDMGLSKGVGFIRFDTRQEAERAIKELNGTTPKNATDPITVKFANNPSSAAKALGALTAYITPSSSRGRFPATATAIHTSGAGRFGYNPMANDLLTNAIIPGNTMSGSGWCIFVYNLAPETEDNVLWQLFGPFGAVQSVKDQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAI4</accession>
    <name>T1GAI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000241-RA">
        <property type="protein sequence ID" value="MESCA000241-PA"/>
        <property type="gene ID" value="MESCA000241"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006532_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576762" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="41"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000241-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13583" checksum="A765930680EDBBA0" modified="2013-10-16" version="1">MKMSGKQLTIYLLLALTRCVLSEGDGCPLKCSKADCDRELLPDCGTGTSIYPYVHEKNCRKYTECISGKEISVECASNQYAKISGSSVQCITYDDNNPPSCIASPKNVCQRVGYLPDPDDCKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAS6</accession>
    <name>T1GAS6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAS6"/>
    <dbReference type="STRING" id="36166.T1GAS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000337-RA">
        <property type="protein sequence ID" value="MESCA000337-PA"/>
        <property type="gene ID" value="MESCA000337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009276_1_0_1"/>
    <dbReference type="OMA" id="IENESSC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="150"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="346"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="443"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="504" mass="55531" checksum="EB2C882A12022D3C" modified="2013-10-16" version="1">MALNIKLKEAFEVEKQYGAFYTGGNICWTSDGSQFLCLENNKINVVDIDSGEVVQNIAAPKEAEEDLEQDTLYTFDLSSDDESVITAHKSGLLKLWSKINGEQIKIWKSGHKGPIPRVVFRSKGGIVATGGSDSTVRVWDYEKKACLSALRGSQGVVSLVVFHPDVSKSFIYAAGDDNKINCWNYATKDHQKSVIPLYEALEGVVVLPKIGKLPNGFNLESPDNKDHVFAAIAGESGKIKVWECTGSKIVYSQTESYVSKAEEEGGLAITQLLHCPKTTQLAVVTADHNILVHNISTFHCSKQLIGFSDEILDICFVGKKSRYLAVATNSNDIKFYDTTNMNCQILKGHTDIVLAVSSHKNFLLSSGKDHTIRLWEVDPGTFKVKCLAKGSKHTSSVGSISFGKVTHTICASVSQDSCLKVWDIPKKFEDQEELLSLECKATQIAHEKDINCVTMSPNDQLIATASQDKTAKIWSASKLELLGIFKGHRRGVWSVRFSPVDQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAZ4</accession>
    <name>T1GAZ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02072416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAZ4"/>
    <dbReference type="STRING" id="36166.T1GAZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000412-RA">
        <property type="protein sequence ID" value="MESCA000412-PA"/>
        <property type="gene ID" value="MESCA000412"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_802401_0_0_1"/>
    <dbReference type="OMA" id="DDRVRGQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000412-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="347" mass="39579" checksum="91FB0DCB480C7B2E" modified="2013-10-16" version="1">MVWTVFNIVSQQSAAINFHGVLITSLDFNWKWINSSLYNFIGSKTVQNPRTKRIINRLFSHYYFVYYLGIFLSKIVPPMIRAETSCFGKNGCYPAVFGALGSIFMMSWIVFLIGKIYYKDERISDENVILKFFGCIKHALVTKMKSSSSEERKEHWIEYAEGRYSLEFIDDVHTVIKITKLFIPLPIYYALLAQQDSSWTFQASQMDTTVFGFRIEPDQAKAMGPVFLFTMIPIWTYLVTPILKTCDIEISALKSMVVGGFVSALSFICAGILQIEINSSVEKTVNILWQIPQFFLIMFGELLLSIPGLEFAFKQAPESMKSVLTAAFFINNAFGNLIVVLITEIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCY6</accession>
    <name>T1GCY6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TRC8-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02141651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02141652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCY6"/>
    <dbReference type="STRING" id="36166.T1GCY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001159-RA">
        <property type="protein sequence ID" value="MESCA001159-PA"/>
        <property type="gene ID" value="MESCA001159"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2284470_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025754">
        <property type="entry name" value="TRC8_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13705">
        <property type="entry name" value="TRC8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="TRC8-like N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001159-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11512" checksum="38F80DB7EC24160D" modified="2013-10-16" version="1">MKLALEIMKFILDCEVSSLVSSACIFMLWTRHLVIVYMFLASVGLIFLSYWSNVSALTSMDNGTYLIEDLLSLNMNRLLDPGGVAISILPHLVAQWFMGFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD12</accession>
    <name>T1GD12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED17</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02090229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD12"/>
    <dbReference type="STRING" id="36166.T1GD12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001191-RA">
        <property type="protein sequence ID" value="MESCA001191-PA"/>
        <property type="gene ID" value="MESCA001191"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028003_1_0_1"/>
    <dbReference type="OMA" id="HMSYEPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001191-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="587" mass="65984" checksum="CD4DA400458A6076" modified="2013-10-16" version="1">MSVNISVETPNENQIHEIAYDGTETYQPKSSLEDLKKEDNKDGVPVDQFQPSLWPWDSVRNKLRDALTEVCVLQDVLSIAKDRKYLILDPIQQEPPESKPMVQVYARKKALAQAASVLINGADRLKASNVEQKNKSNFHIELLRLRQNWRLKKVSNGIIGDLTYRKAGSKFWHNGTFEVTKSEDDPEAEEEAASGPSTPTSTIPPTIKQTSAIKVNVPSELQGVAYIKVITQKDHEDLCTAKINLMGYGRNTAQQSNLWQTVLERAQNVLFCKELFNQLAREAIQLQAPIPHVVIGNEIRASLFPQVQLIISLVHTISTEKSSPNQDNDSPASGPLGPSKKRMLAGPLAADRFELLEMTKSETILEQIIAQAQHAFLRRRTQYVLDTLARDVRDPQIVSHWNAMNSPTMSCVKINIVTHGYETMHRTALLIHVKDRTLKCIGRDGRVMHLSYEPQELRDLILCQVNQHQITSLLSLAKCLAWQVLSNSNHLGIGSVEPLGNASACLLSSPNGDRMIAVELRSYNSLEARVFIAKSPRQDFFQIPLVPEKIWENLGGHFREVRFDKIEGKSFLMKMEYLMASLTSASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDL7</accession>
    <name>T1GDL7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurotransmitter-gated ion-channel ligand-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Synaptic cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02085867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDL7"/>
    <dbReference type="STRING" id="36166.T1GDL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001406-RA">
        <property type="protein sequence ID" value="MESCA001406-PA"/>
        <property type="gene ID" value="MESCA001406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018074_7_2_1"/>
    <dbReference type="OMA" id="VWNASEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577244" description="Neurotransmitter-gated ion-channel ligand-binding domain-containing protein" evidence="11">
        <location>
            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="12">
        <location>
            <begin position="26"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="16869" checksum="A925D219917AB61D" modified="2013-10-16" version="1">MKQLWIFGFLFLAFITLHSVNSSEDEERLVRDLFRGYNKLIRPVQNMTQKVAVRFGLAFVQLINVNEKNQIMKSNVWLRLVWYDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLIYPSGEVLWVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDM5</accession>
    <name>T1GDM5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001414-RA">
        <property type="protein sequence ID" value="MESCA001414-PA"/>
        <property type="gene ID" value="MESCA001414"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010718_0_1_1"/>
    <dbReference type="OMA" id="VFWIRVL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF56">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="33"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001414-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="296" mass="34197" checksum="79AD1D4C2F072293" modified="2013-10-16" version="1">MYQDIIPRMEILLKDSHFGAKMYYAQRQPIYTIAFEDLKVKGFTTAVRQDLLDEEHCRMIMSKLGQFHATSMVLAQRDPSVINKYTGGMLGSVTVNQSDTFKKMFGGQLRYLGNLAKTWPGYEQIGEKLLRFYDNFNNNLVNSAARRPTDITVLNHGDLWVNNFMFKYGQGQRPIDSLLVDFQLCYYGSPGHDINFFLNSSVKLEVLKRLRESFVETYYNSLKSTLEECQFEKIPTLADVKKQVRSRERVGLFALFAFLPIVTMDEETSKESSIDNLSNEENANKVFSKIFKNEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDU2</accession>
    <name>T1GDU2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02022219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDU2"/>
    <dbReference type="STRING" id="36166.T1GDU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001492-RA">
        <property type="protein sequence ID" value="MESCA001492-PA"/>
        <property type="gene ID" value="MESCA001492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1115467_0_0_1"/>
    <dbReference type="OMA" id="CDPMVAL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF2">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="283" mass="32026" checksum="84DA56C78C01799B" modified="2013-10-16" version="1">MECAAYCVSRVQSVTVVCKEDLPLQNAFGTKVAQRIMKLFEENGVKFITKAQVERFQGRICDVYSVILKDGSSVPCSVVILGTGTILNTKFLQRSGLPINEDGSIDCDWNLKTEIEDIYVGGDIANAPIFPANRKAHIGHYQIAQYHGKMAAWNMIGEKDEESLKIKTVPFFWSMLFGKSFKYSGFGISENVRIHGIFYFDENEKVIGISSCGKDPVVAQFAEFQRQERALYKLDLEDDPLAWTKDILCPTKKVENVFDNLCPFLTDAYNEVENEYFKLQNTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEA4</accession>
    <name>T1GEA4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Jun subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEA4"/>
    <dbReference type="STRING" id="36166.T1GEA4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001662-RA">
        <property type="protein sequence ID" value="MESCA001662-PA"/>
        <property type="gene ID" value="MESCA001662"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057007_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14696">
        <property type="entry name" value="bZIP_Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005643">
        <property type="entry name" value="JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF35">
        <property type="entry name" value="TRANSCRIPTION FACTOR JRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03957">
        <property type="entry name" value="Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="179"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="25360" checksum="7340E4EF5943A53A" modified="2013-10-16" version="1">MDENGQSQSVPITTNNMKRPISLELNSGKPNAKKPKLSGIPLQNTFSLDSTNITTPDLEKIILPLLPTPSNNVRNVLYATNQNPTNEKEEFSMGFEKALQNLYKNDSQQQIQQQQQQQQNQQQQANSQTTSTTHTNGMSGGSFTYTHLPDEHFPVVVKEEPSANSTPPHSPIDMETQEKIKLERKRQRNRVAASKCRKRKLERISKLEERVKCLKGENPNLVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEP8</accession>
    <name>T1GEP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02162906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEP8"/>
    <dbReference type="STRING" id="36166.T1GEP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001819-RA">
        <property type="protein sequence ID" value="MESCA001819-PA"/>
        <property type="gene ID" value="MESCA001819"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001918_1_0_1"/>
    <dbReference type="OMA" id="LQCNGST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18491">
        <property type="entry name" value="BACK_ABTB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18297">
        <property type="entry name" value="BTB_POZ_ABTB2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000030">
        <property type="entry name" value="Ankyrin repeat and BTB/POZ domain-containing protein BTBD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059008">
        <property type="entry name" value="ABTB2/3_histone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052089">
        <property type="entry name" value="Ankyrin-BTB/POZ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46071">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46071:SF2">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26281">
        <property type="entry name" value="Histone_ABTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="658"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="967"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="119"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001819-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1141" mass="125442" checksum="AD46D25C63D0E1B1" modified="2013-10-16" version="1">SSAFSPQLQNVGLPQRQTPQFPLPHESGRIQSPASSTAAPRISKEVAQKITNEFQQSEAIQTASLVAERDLSSLPAVPNNVVLQRPTPCRPIPGPVRLTVQSTPVKSHSTMISSMLSFNQQSKSHDQTSQNSRTHNHDFKPLQGVQEGHSSSDENRSSGHASMSDTGGVGSSSPTGVKLGPLPEDRLAADVSSKSTKSKSNTNHSRSRHRALPGKAPWSGTGLEDIKHAIKQMTIRSQTSTSTYSSLSAGSESSEPMRRMGRYSSMETVNTNVTTADEFIWVDSQNRLVEVQHTPWTQNCILKRVLVRISREIQRLSSGFGLCSKHEVVTAFKIILCPALADSCIKACLRAAAMFAAPGDSALKQSKSSRAGLHFPVGKFHRWMADSRLGRFIHEYAAVYLCAGIENMLEEILLQCFSTITDNTTTLTTTLLEHSIGSSADLWGLLQSFNHLNAGRIASGALTMPRWASQSSIGSESLNHTHEKQSNIHMEPCLLTTCVGNMPELKDLVNRAQQKFQHIRLSNAAIVCLFYFMRCSQLEHNDGNGNARNASGNNVQELCNERAYVVLPPLIEWLRVASAHAEYRSGVLVDKDDIMQAARLLLPGVDCPIRPVEETKEFGRRATIALAFKMMLTGRAELLAQASTILPPTTRYDTLNHNGMTALMIACIRNDEIAIQSLLDAGCDPNIEIPSVGTPGCPAIHPETQYWSALTFAACKGNYQACRMLMERGGKVEGGSRLSEEKLTLTPLQVASGSTSCEIVALLLAHGANPFLSTTQKDIMCFSGSAQRGCYCAISVASSHGQRASLRKLLSHPLKPGTKDVLSLEEMLAESDPSVRPLGAIPERQPEIPSTLSKTQVKSLQEAMYHSAENNHLDITIELRTLGVPWTLHCWMHALAAAHDLRLDGVIDQLLQDFLQVKNEGTILLLADIFATCFGWETISPIKEAAMEQPVSGARIDPRFVNNPELSDVTFRVEGKIFYGHKIVLVTASPRFQNMLSSKLSDGVNAPTIQINDIRYQIFQMVMHYLYSGGCNNLTVSNSDVLELMAAASFFQLEGLLRFTETKCSEMIDIDNVVAMYIHSKVYNAHKLMEYCQGFLLQNMVALLTYDDSVKRLLFAKKIPNHDVLGGLLTTLQNRIQRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEU4</accession>
    <name>T1GEU4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEU4"/>
    <dbReference type="STRING" id="36166.T1GEU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001865-RA">
        <property type="protein sequence ID" value="MESCA001865-PA"/>
        <property type="gene ID" value="MESCA001865"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1134674_0_0_1"/>
    <dbReference type="OMA" id="FMHHTRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001865-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="28377" checksum="0BCBF383C2FD1F54" modified="2013-10-16" version="1">MNDLSDLILRDGNENQQQILKNVFYRQFLPASDTKYEFLLSAFSLFELNSKQNRREVLQNLWKKCDGYLVLVEEGTYAGFRLINEARDYLLSLGDGEVFAPCAHESKCPRMVDLASKIPCNFQLHYMPLGLGEKVTEHKGYRYTYLVMKKGINSNVERWPRLVQPTLVRSKHTICRMCTAKGNLQEVIFTQSKHGKFAYRCARSSNWGDRLPIEVGDEIVEEKPEKVRKVFVQEDPDDSSIRVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF17</accession>
    <name>T1GF17_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter family G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF17"/>
    <dbReference type="STRING" id="36166.T1GF17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001946-RA">
        <property type="protein sequence ID" value="MESCA001946-PA"/>
        <property type="gene ID" value="MESCA001946"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1055892_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF61">
        <property type="entry name" value="SD03967P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter family G" evidence="7">
        <location>
            <begin position="34"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001946-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="264" mass="30063" checksum="30C76C5241F9C0AD" modified="2013-10-16" version="1">MFLFSGLDSSSCSQCVSLLKMLAKQGHTIVCTIHQPSALIFEMFDKLYTVVDGNCMYQGPTRELIPFLADQGLHCPSYHNPADFLMEVAVGEYERDLNKLINAANKKYYEDSDLYHLSNIRNGIRHVTDHKDQEKKTGLFYKLCGNNKLSLNFNTKNDVEEDPETCESLQPKKGQEILDMEKLIQPASFIMQYFLLLNRLLIGVRRSYFLLIARIVAHVVIGTIFGYLYMNVGTKATTVLGNYVYFYGTVLLLVYMGKMAVVMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFN3</accession>
    <name>T1GFN3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myelin transcription factor 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02044347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFN3"/>
    <dbReference type="STRING" id="36166.T1GFN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002178-RA">
        <property type="protein sequence ID" value="MESCA002178-PA"/>
        <property type="gene ID" value="MESCA002178"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3162649_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000001">
        <property type="entry name" value="Myelin transcription factor 1-like, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF15">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="48" mass="5025" checksum="79555CDCF708FDE8" modified="2013-10-16" version="1">MSSFLSLFVADGSKCPTPGCNGQGHSTGLYTHHRSLSGCPKKDKVSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFY6</accession>
    <name>T1GFY6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFY6"/>
    <dbReference type="STRING" id="36166.T1GFY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002289-RA">
        <property type="protein sequence ID" value="MESCA002289-PA"/>
        <property type="gene ID" value="MESCA002289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848075_0_0_1"/>
    <dbReference type="OMA" id="SRIRDCC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000130">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="12">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="38075" checksum="E5C7F21FD5029B12" modified="2013-10-16" version="1">MYSINLKTCRVCLVEPADEKCSSTYLEFSKDEDLKQKFKAITGIQLTPEDPDKICPQCKVKLNMAYAFRQNALSTHKYLRKFIAKSKSIFEDMKKSVKLVEVYEDYEFLDSEIQEQPKEPASKSEQHLKQEELIIDEVTEPEYELAEVVEEVNIADQEMAENVITDQYFDEIYEVEEEVLESNQETAESLVTEEIEEIIEDDDLFNEDTKSTLGNRDKDMSMSDSDGGILQCDECQKQFKTKTNLKVHKKLHTGDKPFACEYCQKRFATKGVLKQHVYIHTAKSHINVNTVTEVLHKARVWWLTREDTLAKNHIHVHIVNFILGLETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFZ3</accession>
    <name>T1GFZ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02088132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFZ3"/>
    <dbReference type="STRING" id="36166.T1GFZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002296-RA">
        <property type="protein sequence ID" value="MESCA002296-PA"/>
        <property type="gene ID" value="MESCA002296"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_5_1_1"/>
    <dbReference type="OMA" id="CINGGKH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF172">
        <property type="entry name" value="LIPASE MEMBER H-A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002296-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="25281" checksum="A147651D68412D31" modified="2013-10-16" version="1">MKNVAKCLAESLTFLRDSGLKIDKTTCVGHSLGAHVCGLISNFLKFRAERIIGLDPARPLIKPGNMNRLDSGDAKAVHVFHTNAGYYGETGKVGHVDFCINGGKHQPYCQYTSNTNLCSHIWSICYLAQSIFQGFEPYAEPCSRRCPSGLRGLRSLRGRNLARYAVRNAIPMGMTTPMNAYGSYCIKDDQPPFCPTSPDSIGDQRCCLDYPPEDVDENTLRRRRFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGC9</accession>
    <name>T1GGC9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4780 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02016899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGC9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002445-RA">
        <property type="protein sequence ID" value="MESCA002445-PA"/>
        <property type="gene ID" value="MESCA002445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_795217_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031961">
        <property type="entry name" value="DUF4780"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16012">
        <property type="entry name" value="DUF4780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4780" evidence="1">
        <location>
            <begin position="233"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="349" mass="40103" checksum="E167D682D70C89A7" modified="2013-10-16" version="1">MFIIFKYFEFILQHINGFTYVSRHGPPYNRFSPIIGSSLRMDNFSSKSWDKNMDYIKQVKQQTSNVGSSRSIPDLEQKRLIVDNSAKLIKSYKSKLRKARFFVEKTKKTIASFTPLQLSEHRNDLWAKEVLAKEEERLSKNISLNSFVKDPSQAELARKTDRLVDVIGHPVKRKSDMKDISNLAQKTMKTSSPSNNQISRVVPLAKAPSEIPTIRYGKEDTNTNRVDCSKITQQDLKVSIVDTGASDLNISTTNFSLVEEGIQHEMYKRICEGESFAPFQMKERYMGFRIIECESEAALIFLRKAVSNLGELWSSAKLELRHLNELPVSVHIKVNLPVRSIDMKKIMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGV8</accession>
    <name>T1GGV8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGV8"/>
    <dbReference type="STRING" id="36166.T1GGV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002639-RA">
        <property type="protein sequence ID" value="MESCA002639-PA"/>
        <property type="gene ID" value="MESCA002639"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913033_0_0_1"/>
    <dbReference type="OMA" id="YFISCAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF129">
        <property type="entry name" value="PROTEIN WHITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="9">
        <location>
            <begin position="126"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002639-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33522" checksum="F2D390B12E563CF8" modified="2013-10-16" version="1">MFQNLVSGVAYPGELLAVLGSSGAGKTTLLNTLAFRTTKGVQLSPSTLRMLNGNPVEAVEMQARCAYVQQDDLFIGSLTAREHLIFQAMLRMPRNTSYQRKLERVDQVIQDLSLGKCKNTIIGVPGRTIAELPLFLTVPFVFTSIAYPLIGLRPGVDHYFIALALVTLIANVSTSFGYFISCASTSTSMALNLGSPLIIPLLLFGGYFLNSRSVPPYFKWLSYLSWFRYGNEGLLINQWSDVQAGEIECTNSNSTCPSSGQVILETLNFQASDLPIDFIALVSLIVGFRFFAYLALLIKTKKVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH73</accession>
    <name>T1GH73_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002763-RA">
        <property type="protein sequence ID" value="MESCA002763-PA"/>
        <property type="gene ID" value="MESCA002763"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415767_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002763-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10511" checksum="899FDF172FB08DF1" modified="2013-10-16" version="1">MSVYFLGKSRKPQSFKTTEQKKKSVKNCCALEKFGKLFCSIENAMDSESTFCDCCELRKGCIIWSYRPGFLIPAMQLHLCLYVLQGTSTSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHK8</accession>
    <name>T1GHK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uracil phosphoribosyltransferase homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHK8"/>
    <dbReference type="STRING" id="36166.T1GHK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002912-RA">
        <property type="protein sequence ID" value="MESCA002912-PA"/>
        <property type="gene ID" value="MESCA002912"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_1_0_1"/>
    <dbReference type="OMA" id="NLFCTPM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000026">
        <property type="entry name" value="Uracil phosphoribosyltransferase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002912-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24355" checksum="065DA54E96AF9ACF" modified="2013-10-16" version="1">MCSGLSSTEDSSEYGPNLKILTPNPQIQELQTIIRDKNTTRSDFKFYADRLIRLVIEESLNQLPYSDCHVETPTGAIYDGLKYRSGNCGVSIIRSGEAMEQGLRDCCRSIRIGKILVESDSDTHIARVVYARFPEDIDKRQVLLMYPIMSTGNTVLQSVNVLKEHGVPEQNIILSNLFCTPQAAKTLVRAFPKLKILTSELHTIAPNHFGQKYFGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI68</accession>
    <name>T1GI68_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CAQQ02124231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI68"/>
    <dbReference type="STRING" id="36166.T1GI68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003136-RA">
        <property type="protein sequence ID" value="MESCA003136-PA"/>
        <property type="gene ID" value="MESCA003136"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006812_0_0_1"/>
    <dbReference type="OMA" id="GHTINHR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd20812">
        <property type="entry name" value="C1_KSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000061">
        <property type="entry name" value="Kinase suppressor of Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF253">
        <property type="entry name" value="KINASE SUPPRESSOR OF RAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="12">
        <location>
            <begin position="27"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="326"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="86"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="230"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003136-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="55078" checksum="B76B87E825CA9EB9" modified="2013-10-16" version="1">MENPNILQVPPRSPCLVNPPVMGHTINHRFTTAIKLMATCDLCQKQVFLGLKCTECKYRCHKDCKPNVPPSCGLPKELVNEFIRTTLSHPTPPPSTGNTSPSSSWRGGRKHNHNPDSSSPGSSCNSSSPSSPAPFSSQYSGGSSTSGGGTMSKTPLSATKMVSQFNFPEVTVTSQHGNNNTCTKTLHSSSSSGIDNNSSYEDICSTTPTQNESSTLNTLINSNCSNISTGTFQSTTSINSSATGYSDLSRRRMKTPVIVQMHTDLSDTHKSNDSDKTASQSGSTSASTDSDRTPVRLDSTEEGDSGNWHRQNSTSMKEWDIPYTDLNFQEEIGKGRFGTVHRAHWHGDVAVKILNENYLNDERNLESFRLEVANFRKTRHENLVLFMGACMNPPNLAIVTSLCKGKTLYTMIHIHNEKFALNKITIIAQQICQGMGYLHARGISHKNLRTKNIFLENGKVIITDFGLFSSIKLQYCDLGLGIPYGWLCYLSPELIRSLTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI76</accession>
    <name>T1GI76_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003145-RA">
        <property type="protein sequence ID" value="MESCA003145-PA"/>
        <property type="gene ID" value="MESCA003145"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1391664_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003145-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22453" checksum="64D804317E2D7383" modified="2013-10-16" version="1">MKVFISIIVHSFHIPCPCLDLFNYIWMQWALSVVYKDGPYGPHPPQSAAVIITIWFLCLIMEIPRFLNLYNFCRDTVWIGKVKYLTIYGWKALLPHMHAAMRTTRISSEPAIFLFLSFWIAIRTSSINDVDGVLCLVTPSQHFLVFLALRPLSFCFCSPHSCFECSSGCISDRFPMHLICTKLPITLGVQVWGNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI79</accession>
    <name>T1GI79_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI79"/>
    <dbReference type="STRING" id="36166.T1GI79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003148-RA">
        <property type="protein sequence ID" value="MESCA003148-PA"/>
        <property type="gene ID" value="MESCA003148"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093595_0_0_1"/>
    <dbReference type="OMA" id="ITTEIEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01824">
        <property type="entry name" value="Phospholipase_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035547">
        <property type="entry name" value="Phospholipase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038885">
        <property type="entry name" value="PLB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325:SF31">
        <property type="entry name" value="GH22081P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325">
        <property type="entry name" value="PHOSPHOLIPASE B, PLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="23841" checksum="7690BD148802D9AC" modified="2013-10-16" version="1">MRSLFEKNIESGKLQDRVNHFIPFPCNTTNGRSFVRPTNINKLKPGDIDVIASIGDSLTAANGGMSSNILHVLNENRAISFSGGGKGTWREFLTLPNILKEFNPRLYGYSVDDVLALDKSSRLNVAEPMIMSRDMTYQSKALIKRMKADKKIDMKRDWKLLTIFVGNNDICSDMCHHNPQSNFADQHEKDLESTLRILRDNIPRLFVQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIB9</accession>
    <name>T1GIB9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cuticle protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIB9"/>
    <dbReference type="STRING" id="36166.T1GIB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003189-RA">
        <property type="protein sequence ID" value="MESCA003189-PA"/>
        <property type="gene ID" value="MESCA003189"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1497937_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF196">
        <property type="entry name" value="CUTICULAR PROTEIN 72EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588326" description="Cuticle protein 6" evidence="2">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003189-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19611" checksum="FA4DF25EAD3124DA" modified="2013-10-16" version="1">MRIISPIAIEVLLGALVLVYAQEPTTEAIQPYEFGFNDGLSTRQEQQTADGVVKGSYSYIDAYGEVQKVSYSADAVNGYVVHTDKDSQKVAIPTAATNLDKTKFIQTINEGPKKPVEISKIEVVDGHVVDTPEVAAAKALHFKAFREAEATAANDEKPEKPILRKVPAVVQPKFALLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIS9</accession>
    <name>T1GIS9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 169</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104683">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003364-RA">
        <property type="protein sequence ID" value="MESCA003364-PA"/>
        <property type="gene ID" value="MESCA003364"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_833050_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029386">
        <property type="entry name" value="TMEM169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31777">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31777:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15052">
        <property type="entry name" value="TMEM169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003364-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="38038" checksum="6361967A587990A8" modified="2013-10-16" version="1">FAKNCLLRLQALKRTFKDFIDSKEQTTTAKNSNSDNIYDVQGQHSKSNNGNNTNPNKNTTYSQNSLDAKLSKSQDCLTTRSIKKRVNIRTDDLVMGQRPVPKPGHSSGDIINNYEDIEAGIEAAITNSTAQTPDNAMNSLDRFNTRRSMDSNYLTMTGTIKRGKKKGQSIDLQVNISRDELEKIIDTEAKRKEKSCCVCNLETGLHIFLFSFLCMPFVCLITSVYSFYIGTLTWYNMFTYFTEERSYFHKLFLSPLIILLYPFMILLCTFGLGLYAGISQLSVKFSTWWNEVGDIEKGFYGWLCNFVNLSDCAPYEVNFKFSTGSSQVLYSWDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIZ2</accession>
    <name>T1GIZ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02029086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003429-RA">
        <property type="protein sequence ID" value="MESCA003429-PA"/>
        <property type="gene ID" value="MESCA003429"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1742640_0_0_1"/>
    <dbReference type="OMA" id="MKGVIAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003429-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="13877" checksum="BC7FCFB809517013" modified="2013-10-16" version="1">MEGLIHDGVLFAILALSNAQRQQGGFGGGSQSNFGGSPQGGGGSGGFGGQPGGANFGGSSGGSGLGAGNSFGGNGFGGGAGGFGGSPGGFGGSPGGGGFGGGSTFGRDPSQGSFGPGSRPQGRPGFGGKADQAEGFLQDLQEGFLDKCGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIZ6</accession>
    <name>T1GIZ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02109080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIZ6"/>
    <dbReference type="STRING" id="36166.T1GIZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003433-RA">
        <property type="protein sequence ID" value="MESCA003433-PA"/>
        <property type="gene ID" value="MESCA003433"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326663_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003433-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11629" checksum="BDFC4A3333177322" modified="2013-10-16" version="1">SEKYNKEHFVNSLILPLFFNSIFIVGVYRATCVYLACKVEEFNISIKQFSENIKGDREKAEDIILSNELLLMQHLNYYLTVHNPFRPVEGLLIDIKKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJE1</accession>
    <name>T1GJE1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02070451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003587-RA">
        <property type="protein sequence ID" value="MESCA003587-PA"/>
        <property type="gene ID" value="MESCA003587"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1422992_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.420">
        <property type="entry name" value="AHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003587-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="22153" checksum="739A9A0C4CB406F1" modified="2013-10-16" version="1">MGGPKVSLKYREQLSDYLDKLFGVFNQQNNFKNENSLVTSNYSKLAATIEAKEYYSCEMEEKLKSNTGLKSEDLLKFHNKIKSKAIEKFIKVLKQINVQYQSFLDDLNKSLEELFEIYKTQNETSLVASDDKFKEKHMDIIYNITSSYAEYMNDILDRNANLSSEALEIAHLKISAIKMKELIPFLKHSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJX0</accession>
    <name>T1GJX0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02123220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJX0"/>
    <dbReference type="STRING" id="36166.T1GJX0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003779-RA">
        <property type="protein sequence ID" value="MESCA003779-PA"/>
        <property type="gene ID" value="MESCA003779"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004826_3_4_1"/>
    <dbReference type="OMA" id="MYSCNCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048495">
        <property type="term" value="F:Roundabout binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010631">
        <property type="term" value="P:epithelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010160">
        <property type="term" value="P:formation of animal organ boundary"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048732">
        <property type="term" value="P:gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008347">
        <property type="term" value="P:glial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000729">
        <property type="entry name" value="Blast:Protein slit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000080">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051355">
        <property type="entry name" value="Notch/Slit_guidance"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836:SF4">
        <property type="entry name" value="PROTEIN SLIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836">
        <property type="entry name" value="SLIT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003779-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15302" checksum="2DC5676E5C4F2860" modified="2013-10-16" version="1">MFTIRQYCDSKIKFCTEEFNPCTNGAKCSDHFTHYTCECQPGFHGTNCTENIDDCQNHMCQNGGTCVDGINDYVCKCPEDYTGKYCEGTPMVAMMYPQTSPCQNHECNRVCVSNQIHPVRTTCASVIQVILGNGVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKE6</accession>
    <name>T1GKE6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKE6"/>
    <dbReference type="STRING" id="36166.T1GKE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003969-RA">
        <property type="protein sequence ID" value="MESCA003969-PA"/>
        <property type="gene ID" value="MESCA003969"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1519591_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF17">
        <property type="entry name" value="LD24139P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003969-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="19175" checksum="46D45710CE6DA0FE" modified="2013-10-16" version="1">MLAIRIDENHNALGPNVNLASHNIEVVLYNELFVFFHRDPSTSSRAGKALSENDIVTSLPEESEERTGRLIDMAMQSAVDFFSTHNLNVKVPAETTQEIARSIEEGRGKLKKVAGPLVMAIGAKLITIIPLILGIGGYNANQWSGSNGWSSGSGSYPYARSLSDAQDLAYGKQVQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKH5</accession>
    <name>T1GKH5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein ROP</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02184586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKH5"/>
    <dbReference type="STRING" id="36166.T1GKH5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003998-RA">
        <property type="protein sequence ID" value="MESCA003998-PA"/>
        <property type="gene ID" value="MESCA003998"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009210_2_0_1"/>
    <dbReference type="OMA" id="RYKHIAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051650">
        <property type="term" value="P:establishment of vesicle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003998-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="56445" checksum="B745525D8043B638" modified="2013-10-16" version="1">MALKVLVGQKIMNEVIKYKPPVNPKKAAGAAAKPGGVEWRVLIVDQLAMRMVSSCCKMHEISAEGITLVENITKKREPLPTMEAVYLITPSEESIRALMSDFDHPSRPMYRYAHVFFTEVCPEERFNELCKSCAARKIKTLKEINIAFLPYECQQIATLCATLGEYPQVRYRVDWDKNLELAAAVQQKLDAYKADEPTMGEGPEKARTQLLILDRGFDCISPLLHELTFQAMAYDLLPIVNDVYKYAQSPNGPDKEVLLDENDELWIEMRHQHIAVVSTQVTTNLKKFTDSKRMSASDKASMRDLSQMIKKMPQYQKELSKYSTHLHLAEDCMKFYQNNVDKLCKVEQDLAMGTDAEGEKIKDHMRNIVPILLDQAVSSFDKVRIIALYVMIKNGISEENLTKLFTHAQLTPKDQDMVRNLNFLGVNVYADNRKKNYNIPRKERISENTYQMSRWTPVIKDIMEDCIEDKLDTNILLICKEVLEMPSIMLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKR7</accession>
    <name>T1GKR7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175069">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKR7"/>
    <dbReference type="STRING" id="36166.T1GKR7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004094-RA">
        <property type="protein sequence ID" value="MESCA004094-PA"/>
        <property type="gene ID" value="MESCA004094"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827529_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004094-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15586" checksum="68A09A541D61D2AD" modified="2013-10-16" version="1">MYTYIIIFPYFQDSFLNVRVKVPQLNTLSSIQGANLVNGIGFEVTDPDVSGEDIFGRLVPMEAHEGSSLYSEEKAQLLRTIGAKVEQKNVELTTYMSSLNLENLNISEERANKIPQGIVDRCAALNANTEAIPDLISSMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL34</accession>
    <name>T1GL34_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL34"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004221-RA">
        <property type="protein sequence ID" value="MESCA004221-PA"/>
        <property type="gene ID" value="MESCA004221"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1557044_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004221-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="19486" checksum="07FF32FA58354515" modified="2013-10-16" version="1">MVWTQQLEKDVPLPTTEVRSAIQRLKTNTSAGSDGIPAELLKAAGINFIPAFHQLLEKMVWTQPLEKTQNLPFEENRGNLLTSTSLHHEITASSALSTKNETRVTAKTTKKSAQHSIQNRRIHRDKAPETPLLRSKQLSRKTKVRLYHQLVFAVLLYGYESWSLTSADEQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL59</accession>
    <name>T1GL59_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004249-RA">
        <property type="protein sequence ID" value="MESCA004249-PA"/>
        <property type="gene ID" value="MESCA004249"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624821_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004249-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9361" checksum="3077725F2C115E70" modified="2013-10-16" version="1">MQTVDDDQLVKDIENNLPSLPIAYWSKIKIFLNKRLTNVQNIHQYSNWNSTIFIGKHYVLQMGHFNYMEHTMTYEKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLA9</accession>
    <name>T1GLA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004308-RA">
        <property type="protein sequence ID" value="MESCA004308-PA"/>
        <property type="gene ID" value="MESCA004308"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673922_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004308-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8232" checksum="02E8C8C8952283D9" modified="2013-10-16" version="1">MLKCAVSICSQKMSFTSMGSWGHILVISCVDPGVPSCYLNSLSILVSVSIAMYNFPVSYPKHFVVFQSHSVSEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLJ3</accession>
    <name>T1GLJ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acyltransferase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLJ3"/>
    <dbReference type="STRING" id="36166.T1GLJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004397-RA">
        <property type="protein sequence ID" value="MESCA004397-PA"/>
        <property type="gene ID" value="MESCA004397"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_957408_0_0_1"/>
    <dbReference type="OMA" id="ESANCAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="2">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004397-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="291" mass="33974" checksum="C4149CABDA36C52F" modified="2013-10-16" version="1">MKAISMMWIIFGHTYMWNLLAPLDNPIILQESWPKTFFSMFVQQSVFSVDTFFLLSGFLLAWTAFREIERRKGKLNAGLMFFHRIIRTAPLKIVLVFVVVSVFRYFGSGPFWETYSRPLQFYCEENWWKTMLFIQNYAQPEEMCLGHTFYIAIDMQLYFLSPLVIIPLWKWGKKVIPALIILCLLSVVAIIIIFYHEGYDEVIGVQEFENSNILKLTYYATHTRFPVWLIGIIVGYLMYTTKTKPLNIPWYVIISGWAVCLGTMLAIVIGPINVTKEGHNKSDIRPLFTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM20</accession>
    <name>T1GM20_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM20"/>
    <dbReference type="STRING" id="36166.T1GM20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004588-RA">
        <property type="protein sequence ID" value="MESCA004588-PA"/>
        <property type="gene ID" value="MESCA004588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726264_0_0_1"/>
    <dbReference type="OMA" id="LICCIAS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024882">
        <property type="entry name" value="NUP58/p45/49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437">
        <property type="entry name" value="NUCLEOPORIN P58/P45 NUCLEOPORIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437:SF2">
        <property type="entry name" value="NUCLEOPORIN P58_P45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15967">
        <property type="entry name" value="Nucleoporin_FG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="381" mass="42988" checksum="01B228D9FD2AD2C5" modified="2013-10-16" version="1">MDDFLFMEEHIPGSNRFSHYHGNTRKVEECEDDGDEIGSRFLYELDVLHYRQRLHSSYIEVVQIPCFNATLLLICCIASIFAVLFALDSSTFVVESGESLGLTEELSSFTFGFLVDKGLTTTTVAPPAFVGLGGIDVNTSSQPKQGDQTQANVKVKETLVPDEIAKTVEDLKAYIKQQKTLSSDIGRASLSKLYSVTNDIQTINIALQEVTNSVEQTYSQTKSLRRETSKAIQSAEIAQRTNDTPSGLQFENTAPFFYFVSLVEKYEQDLIVYRNQITAIENHMKSIMNPQGINPTELKNGLQHINECFISLAGRLHEIHQKVETQKEQFLNLRKYRLRDCENNKLQSKTAADNINGFPNFTPNHIEKESQIQELLLSLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMG1</accession>
    <name>T1GMG1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="3">2.3.2.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02007000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMG1"/>
    <dbReference type="STRING" id="36166.T1GMG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004735-RA">
        <property type="protein sequence ID" value="MESCA004735-PA"/>
        <property type="gene ID" value="MESCA004735"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1201023_0_0_1"/>
    <dbReference type="OMA" id="RWNSENF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF6">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588406" description="glutaminyl-peptide cyclotransferase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="7">
        <location>
            <begin position="130"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004735-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="231" mass="26425" checksum="D696F7A3284DDC2C" modified="2013-10-16" version="1">MVWFSMFCLLKILPTHENFLSVGYQIITELFDVVIGDVILAMLSNGPNPPTPHRSRPISNEHLKSINDLSDIGKFKETLEPILVPRIVGTPNHEKVRNYIVDEMKRLNWSVELDTFDDKTPNFGTKTFHNIIAKLNPNAKSFLTLACHYDSKYIKDVEFVGAIDSAVPCAMLLHMAERLNGYFESSKTSDLSLMFVFFDGEEAFQEWGPEDSIYGARHLASRWNSENFFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN08</accession>
    <name>T1GN08_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN08"/>
    <dbReference type="STRING" id="36166.T1GN08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004947-RA">
        <property type="protein sequence ID" value="MESCA004947-PA"/>
        <property type="gene ID" value="MESCA004947"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1327711_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004947-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23568" checksum="79736D4998F64D7E" modified="2013-10-16" version="1">MMNAMDTVQLFSKGEYMVIYPDMMTYSQTEAEKYLWETGKMQCDESETFKRRARSLLVVVSTPPTENYENFTKIVREYNAKEPFNFTTPSLFHLKNISKKCSDHMASSIPTFARTEPPDTQSENDAGVGLKQDANAQMLIVRSIAAVSKLYAMKSEININFLQQSFFDFKKSPEGGIHVHISNGHNENFDDFGSRIQTFVLIVGIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GND9</accession>
    <name>T1GND9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GND9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005086-RA">
        <property type="protein sequence ID" value="MESCA005086-PA"/>
        <property type="gene ID" value="MESCA005086"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592518_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005086-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9467" checksum="C83D5C01B1391BB3" modified="2013-10-16" version="1">MRYNENSLSNYFILFPNHPPKDYDVGDEVKFVLLSDIISIWFVYTYFGKNRPVTIVSFYEKTTIQSRALPGPFHKLRVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNH3</accession>
    <name>T1GNH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mortality factor 4-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNH3"/>
    <dbReference type="STRING" id="36166.T1GNH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005124-RA">
        <property type="protein sequence ID" value="MESCA005124-PA"/>
        <property type="gene ID" value="MESCA005124"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039566_1_1_1"/>
    <dbReference type="OMA" id="GLQTYFD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18983">
        <property type="entry name" value="CBD_MSL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.30:FF:000001">
        <property type="entry name" value="Mortality factor 4-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000024">
        <property type="entry name" value="Mortality factor 4-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF48">
        <property type="entry name" value="MORTALITY FACTOR 4 LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038133">
        <property type="entry name" value="HAT_Nua4_EAF3/MRG15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="21"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="70"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="351" mass="39903" checksum="F753D41830FFE9FD" modified="2013-10-16" version="1">MTPKFKFADGEKVLCFHGPLIYEAKLLKSQVTKDKQIKYFIHYAGWNKNWDEWVPENRVLKFTDQNCQRQKEVSKQHAAASKNNRKSTTKGKKGDGKDGDSRASTPSKESGSSTPTVPSSRGRGSKSASKNSADIPDEEVPKKKRGRADTSNTTNNTSNNTTPTSNNTTSLTTNSSTAKTRKNIIKIPLPVELKQILVDDWDAINRQRKLLEIPAKITVEDILENYVAFKKQSKTSNINNIRTDITAGLIEYFNVMLGSQLLYKFERPQYSETLQAHPNTPMCKLYGAYHFLRLFVNLGKMLAYTALDEKYIQTLQTSISDIFKYLIKNSSQYFDSKNFINSSPEYHRRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNK4</accession>
    <name>T1GNK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005158-RA">
        <property type="protein sequence ID" value="MESCA005158-PA"/>
        <property type="gene ID" value="MESCA005158"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_842754_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588404" evidence="1">
        <location>
            <begin position="17"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005158-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="330" mass="38413" checksum="BF62C678E4913526" modified="2013-10-16" version="1">MKYVFCFLIFIALAKAIKIQKIESNGVYFKDLGYVERNFGVWNCIIQKIDDFKKGIKYKSVNNSINFLQISCFENILNSSENLKNEDIFSFENDFEKCLMNIKSNNCNNLLTLDQLRQRIQSLKISVDSGSVFNNLWKTCKISRRKTFNGKIEISLKFQILDESRFFASTLENVNLERLNREILINNFENFKGLQQRSCIVELFNNQTGKHCNYVRLSRTKEMFQNIGDYQYLFSILDGSHYSFKCPGMEHNIGSNELIENTGILKLNQGCSFRTQYTQLEAQKVDLYFQNGNVINPKAPIDSLEIYNSSTFSPIDLLRNDFDFNSSLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ09</accession>
    <name>T1GQ09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02025760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ09"/>
    <dbReference type="STRING" id="36166.T1GQ09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005706-RA">
        <property type="protein sequence ID" value="MESCA005706-PA"/>
        <property type="gene ID" value="MESCA005706"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_971710_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005706-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="33230" checksum="422013EA132B9023" modified="2013-10-16" version="1">TEESSFQDFLRTAELAGTEFQAEKLNLQFLIPKANAGVLSDNQTRELREKHKANEDLLKIPRRPKWTAETTGEELQKAENEAFLEWRRGLSVLQEDEGILMTPYEKNLEFWRQLWRVVEKSDVIIQIVDARNPLLFRSEDLEKYVKEVDKNKMNMILINKADLLTEQQRVHWADFFDKEGMRVAFFSAILVEEENQSAAKDVEIDEKTKIEIPESDDDDDDDESDEEEVEDKSEDIKESTRKLSESLSEIEEQVSDILLCDCPGLVMPSFVLTKADMVLNGILPIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQQ8</accession>
    <name>T1GQQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02065370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQQ8"/>
    <dbReference type="STRING" id="36166.T1GQQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005971-RA">
        <property type="protein sequence ID" value="MESCA005971-PA"/>
        <property type="gene ID" value="MESCA005971"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031900_0_0_1"/>
    <dbReference type="OMA" id="YEWFHWK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15585">
        <property type="entry name" value="PHD_ING3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000103">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042020">
        <property type="entry name" value="ING3_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF103">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="342"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="109"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005971-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="399" mass="44412" checksum="DAC25763C80E8568" modified="2013-10-16" version="1">MDSLDRRSKHFFSECKRGDLKDQDADNEFQNLRKDYYKVLEEADEKVALSTQMHELVERYLRRLDNELFKFKCELEADNNGITEILEKRSLELDGGSSMSHLGTQKENRYFGSIGNNHHQSGSSSMSLQQKEHRYRHRAEKRRDSGSNAVPAQHTSTPNYPTSAPSTPSIVRTASAASTNANANHAQPPNVAQNINAPVFNSNIAIAAAASQAIVATQQMQQGRRTASLKASYEATMGGAVNGPHEIIIGRELAGATHNAIQAVERDHQRKKKKIPSIASNQHPSTSAAAIQQPAPIQTANLAIPQVVTNPHIEVVNTRIENGLVIEQTADGEEWSYDPNEPRYCICNQVSFGDMVACDNDTCPLEWFHYSCVGITQPPKGKWYCPKCTQSMKRRNRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR50</accession>
    <name>T1GR50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006123-RA">
        <property type="protein sequence ID" value="MESCA006123-PA"/>
        <property type="gene ID" value="MESCA006123"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415782_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006123-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10246" checksum="7E6E88E93C7951FF" modified="2013-10-16" version="1">MKDVFFPAYDMKVVLGDLYAQHFLGTIGSLHDESAAKTSKKKKKKNAETPFSSSTLRCNLIRIDLNKTCALIGLFSLLPSFNQHIIQVSISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR88</accession>
    <name>T1GR88_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02045400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR88"/>
    <dbReference type="STRING" id="36166.T1GR88"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006165-RA">
        <property type="protein sequence ID" value="MESCA006165-PA"/>
        <property type="gene ID" value="MESCA006165"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1620927_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006165-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="18716" checksum="321741A1B454DA98" modified="2013-10-16" version="1">MLTTSMLWKHEIGCRKGGYGQMVVKPCKCSQAEEREKSQPPGPESHHGKVQLRGYEQFIRYTLATVHAVRKSIRGYIVEHNVSALALVDVKNCEGEIDGLTDNNIPRRLGSKRVIKIRKLYNLVKEDDVLVLQRKRSRLALRKKHQVASKEAAANYLKLLAQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRB3</accession>
    <name>T1GRB3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRB3"/>
    <dbReference type="STRING" id="36166.T1GRB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006193-RA">
        <property type="protein sequence ID" value="MESCA006193-PA"/>
        <property type="gene ID" value="MESCA006193"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278940_0_0_1"/>
    <dbReference type="OMA" id="KVESWID"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006193-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24698" checksum="33DFF1DE46F7743D" modified="2013-10-16" version="1">MLFKILVVNQYYGADILMEYVIKGNAAILKCSIPSFVADFVKVESWIDEEGIELRASENYVVSQYYITEAENEYVIKGNSAIVKCKIPSFVTDFVSIEAWVDEEGNELWKNNVTNYVVIQSYEAEADNEYVIRGNSVIMKCEIPSFVADFVFVIIVVQQFYETRVIDEFILKGNSAILKCIIPSFVADFIEVYSWLDNEGVEYLTDLVEKGNTIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRP5</accession>
    <name>T1GRP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IFT172 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRP5"/>
    <dbReference type="STRING" id="36166.T1GRP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006333-RA">
        <property type="protein sequence ID" value="MESCA006333-PA"/>
        <property type="gene ID" value="MESCA006333"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031325_1_0_1"/>
    <dbReference type="OMA" id="ELEMAEH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF2">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 172 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006333-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="41891" checksum="A6784A4AD6AED96A" modified="2013-10-16" version="1">MQLKYLQTLIDGQDQINRVAALAWSPNNQKLAVATADRHVLIFDENGERRDKFSTKPSTPGNGKNSYLAIAQSDCIVFVYKFGDSWNDKKVICNKFVQPTAVTTLIWLSSGPIIAGLDNGKVRALHCKTNKSQTLYNGESLTISLSSNSKGNGFLSGHDDGTVIRFYVSDEGAEASGKLLQHHVPPIALAWPQGGVAVGGCDKKIYFYDNQGRQVKVFDYSRSDIERDLNIASSSPNGQAVAFGGFDRVRIYSWSPRLSAWSESVMKEIENIYTVSALTWRKDGSRLALGSVSGAVVLFESVLRRTIWQDKFELIFVAPSQLLVKSLQEPSEQMIIQSKLGLEIDDVRIMGKDNYLLARTEDSLILCDLTRNLISEVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRQ0</accession>
    <name>T1GRQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRQ0"/>
    <dbReference type="STRING" id="36166.T1GRQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006338-RA">
        <property type="protein sequence ID" value="MESCA006338-PA"/>
        <property type="gene ID" value="MESCA006338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_332408_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12289">
        <property type="entry name" value="RRM_LARP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034880">
        <property type="entry name" value="LARP6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024642">
        <property type="entry name" value="SUZ-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12901">
        <property type="entry name" value="SUZ-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51938">
        <property type="entry name" value="SUZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="216"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="314"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ-C" evidence="6">
        <location>
            <begin position="673"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="553"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="697"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="82684" checksum="90E9E3A8396EF815" modified="2013-10-16" version="1">MSSQAIPIKYVNAKDYELNHERIEKEEIQLHQRNEENERNNTISAVPLNNNIKSSKNNESESSKVTTITQPQQQQQQYQPQQQQQENQRPADVVTTRKSSSSASDILASIENQLNIIMPLSKSSKVVTEKLIEFDETAPASEAEDELKPLIIDNHNPVEKPLTTTQFNANAPVFSPSYRTQLLLADKRDSMDSNCSEDTNKMYLSHSPVGSVPDVLEIDTIEPSEMPSDLFSESSLKLILKDAFLLKHVRRNKEGFVSLKLVSSFKRVRQLTKDWRVVGQAIRKKGRRIELNDICTKIRRIEPLPNFDETMPSRTIVACDLPLDKLTIEKVSDMFSQCGEIALIRILKPGMGIPVDVRQFMNKYPEMQEKECALVEFIESNSARNAKNLPYQVYDMVAPKKKTGRKMPNGNAPEGRMTKMIETYKAYEHPHHNYERNRGGSFSAPHNSPPTENKYIQKYQQNHHHNHYNPYQQVQLQQQAAQHIFQRNNSSYNMGDNYNRRFSNGSVTMLEDRRFSSCSEGYSSCSDMSRRPSGYSTDESRRNSAACSDMHCSCGGSRRPSQCSERLSNGSDYGRRFSNGNTPHQLERSYSNPTTEAFLSRRKDSLDSSYDRKLSVGSQGSMCESLNSFHNFQNYRKCSNGFDRKMSNDSHFINGRRLSSDSGYDRRMSFGSDSENYGSPRSRANSFLTTGSQHKCTDNIVRSPMGPDGSKGFASRARKFGQIVPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSK5</accession>
    <name>T1GSK5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006665-RA">
        <property type="protein sequence ID" value="MESCA006665-PA"/>
        <property type="gene ID" value="MESCA006665"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815351_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006665-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7311" checksum="EE6ADBB857014977" modified="2013-10-16" version="1">MMGYSQSSANVIEISKSLENSLLEDSEAAMDIDTEVDKEGSNVKAMDAAPKQGTDQEKTLTYSKCIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSZ5</accession>
    <name>T1GSZ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSZ5"/>
    <dbReference type="STRING" id="36166.T1GSZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006811-RA">
        <property type="protein sequence ID" value="MESCA006811-PA"/>
        <property type="gene ID" value="MESCA006811"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_804849_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="6">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006811-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="345" mass="39169" checksum="69C036FB797FA304" modified="2013-10-16" version="1">MGYRCIQDIVGNPVQQTTINLFYLLNTLQGAKLNHFFYGDLLVPGTIDTALSKCVALIMKAVELQLVVDSCAMDMKAFWSWLSVALVSITDETITDDSNQMSQEGRMNLAEILNNIEEYFSLKENGNGRSKFNMEKIGQYLKHENLKESYTAEMTENNSWLKWLDENKCLENCPKIYMPAKKLSLVQTLDEFTESVLNIFKNAEVVLSKNFVLKKYITLGTGLRNPVFGKFKHSELNLIDLKFYNEEIISICLSAEEEDKKLQTYFVQFSLDLLKHKWIPRQFDGPIDLSNMVGNDVATAFFLELADLDSATKVENDSVAIEVSGSRKVASILSDTPPPRLTFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT63</accession>
    <name>T1GT63_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02110501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT63"/>
    <dbReference type="STRING" id="36166.T1GT63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006884-RA">
        <property type="protein sequence ID" value="MESCA006884-PA"/>
        <property type="gene ID" value="MESCA006884"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145800_0_0_1"/>
    <dbReference type="OMA" id="HNARIVY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF438">
        <property type="entry name" value="CADHERIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cadherin" evidence="6">
        <location>
            <begin position="7"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="6">
        <location>
            <begin position="55"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006884-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12823" checksum="E82BF57EF3F1346D" modified="2013-10-16" version="1">MHTGELTARSLDREQKSRYLLQIQAQDRGTPVMFEDQCNISVIVEDQNDNDPIFEQPKYVTNIPEDIEIGSSVLKVRAIDKDLGHNARIVYSLANETTWLFTIDNKSGLISTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTC1</accession>
    <name>T1GTC1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NUDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nudE family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTC1"/>
    <dbReference type="STRING" id="36166.T1GTC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006949-RA">
        <property type="protein sequence ID" value="MESCA006949-PA"/>
        <property type="gene ID" value="MESCA006949"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1215983_0_0_1"/>
    <dbReference type="OMA" id="CREYINE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007100">
        <property type="term" value="P:mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF1">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUDE" evidence="9">
        <location>
            <begin position="80"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006949-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="26152" checksum="8CED39332C2CE9BC" modified="2013-10-16" version="1">MDKSKEELISENHKLKLEYDEFIEDSKQLEAEMEATIENQKLEKQLESVLEEKEQLKTYCRQLEQKNDDLERAQRILGESIADIRQMLDVAYEKNALLENEVDEKEVLQQKIQRVMDENRDLKQELNVQIIMRNSKTSNSPTTNGDSLSISGTNNILTHATTAINDCNSLKNADNVLNGNTNSFSASSKSSALNVVAEMLKKLNGMESKLTAYRQQVRHQHHLHHPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTK1</accession>
    <name>T1GTK1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTK1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007035-RA">
        <property type="protein sequence ID" value="MESCA007035-PA"/>
        <property type="gene ID" value="MESCA007035"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402161_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007035-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10166" checksum="C39042CE2CE53B99" modified="2013-10-16" version="1">MKASKIPRVSASVNVDQKICLAMIILPATGRPFRISKGIFGKQTLAGMKIVRKCGNILKDTDFPESTENILETHERVGSLTAEACGELFEMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTN8</accession>
    <name>T1GTN8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007076-RA">
        <property type="protein sequence ID" value="MESCA007076-PA"/>
        <property type="gene ID" value="MESCA007076"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349071_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007076-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11438" checksum="AD4DBCFEB94EDE2D" modified="2013-10-16" version="1">MNENGGVTININYEDQKIQKAVYRQMTTDVFLSDHEVSEWKVKALHRKEFPQHFQLFSKILLISFLVAFPSLQVLHSYFTWANSSSPLDWQTIDQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTS7</accession>
    <name>T1GTS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2</fullName>
            <ecNumber evidence="1">2.1.1.296</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(ribonucleotide) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(2'-O-methyl-ribonucleotide) in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67024"/>
            <dbReference type="Rhea" id="RHEA-COMP:17169"/>
            <dbReference type="Rhea" id="RHEA-COMP:17170"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167612"/>
            <dbReference type="ChEBI" id="CHEBI:167614"/>
            <dbReference type="EC" id="2.1.1.296"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.296" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02004347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTS7"/>
    <dbReference type="STRING" id="36166.T1GTS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007118-RA">
        <property type="protein sequence ID" value="MESCA007118-PA"/>
        <property type="gene ID" value="MESCA007118"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_283905_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004483">
        <property type="term" value="F:methyltransferase cap1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120550">
        <property type="term" value="F:methyltransferase cap2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12760">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025807">
        <property type="entry name" value="Adrift-typ_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050851">
        <property type="entry name" value="mRNA_Cap_2O-Ribose_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121:SF2">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51614">
        <property type="entry name" value="SAM_MT_ADRIFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adrift-type SAM-dependent 2'-O-MTase" evidence="8">
        <location>
            <begin position="716"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="823"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="716"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="783"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007118-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1096" mass="127190" checksum="FF67AFCF24792D3B" modified="2013-10-16" version="1">MVFEASRIRTPNAAKVDLEYENFFNKKRTLFKNPEWKLPDISKAFSSGEYNFEDQDDSSIIEMSELLSEVLDKYLSNDFKDMAQNVSLNSSIVKFLKYEFKDIGVTRNWVKGFEILSTFPLIKSGTLNTIHSGDAHGGFINALNHYLHQNFENVNWNWKAFCENPYYEGSTTSKIFNDRFISNTLEHWNFGVDFTGDIETVENIDFFSRKSQEMGNIDLAFTIDCPLVDLEYDYRIRFAGIVASLKALSKGGNAVILLGHNNLFYSRYISLMYFLVLAFEKVHILNPATHPYTLTECFVVAMNFQKSDIIEQYIETMKLKVDLDSWESGPLFPQDIIPKDFLVQINQCANLITNGLISDIKLVLLYYEKPENEISKKRKFILKTIPQKFMKKFKIKEIAKEKYLIQDINLLKAVNRTKESLDLQFCRNQQCNLTAETLSELVEWPYEVEETSILNLNPVELCLEYGKPIDRILKTMFVCPASMFFYAFLIEEECKASLFSSFPKGEVSCVFDDSISIIYEYQQHFGNAEKTFFFSIFEKWIELKPKKVYFKNVLFLTHFSVSFLRILAQFYESIKVNMNGIIVLENRLPQNVDSNLIDDLKTHFEKSFDFIMCLINLKILKEGKFFELILNYNSQLIKHYVIELVQQFVALFDKKSKEWKLISPEECFQGSEYSFQSLQILKEQLNSVKSQLNNYAIEEWHQHTSQRNPGKAVPSWNWRASTLNPYYEGNSLNMITDDRLLLYTLENWIFAKDFTGNIMCTKNIKEINEECLKMGSIDLVTADGSIDCTETPDIQEEVVFNLKYSEICAALNMLSEGGSFVIKVFTLFEACTVSVLYLLNCIFKEVSVFKPATSSCGNSEVYVKMLEKVDETFISTPIFPIEAIPKEGLVKWPYSSSSINFCSLGTAIEIEIVTCKAIDNVLNSKFIGFELLKHIRKEIENNFGKSLFSYFTKDPIEFGADNKKIMFKMKDSFGKSEKDFFQQILLFLRKHKPEKVYLRNFIFLTHFSVSLLRILAAVFEDLWFTDSGDIILDKCKHSEWNVLNSVECEENIFSFLDIKILHKNDFNEALTNFNDQLLFRFIENALITIEKNLSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTX9</accession>
    <name>T1GTX9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007178-RA">
        <property type="protein sequence ID" value="MESCA007178-PA"/>
        <property type="gene ID" value="MESCA007178"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052277_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13344" checksum="472E271963FC607A" modified="2013-10-16" version="1">MVNAQNATAAAAITPTYITTTTQPQRSKCSPVCDPTCPLNTPHTPHNTSPPKKQMKTIELKTEVKTPEPELISCKKESDDEVSSLDSPINFKEECLDDFGDWEHGLTIDEYLQMNGNFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTY2</accession>
    <name>T1GTY2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02181516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007181-RA">
        <property type="protein sequence ID" value="MESCA007181-PA"/>
        <property type="gene ID" value="MESCA007181"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534304_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577132" evidence="1">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007181-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20278" checksum="A85C1EA1A13294D0" modified="2013-10-16" version="1">MSWLFVCWYWLYHCGKGQFMRHFSKDCPQENKNELLRLLLIAAANYQKVIINTTGNHSLKGKTTPPLQTKFPPASLPPKGQNILLSRDTILYRSSLLCSLKNNSLSNRRCFCGLRSLQSRRKHFRRMYCMGANFLIRYNRKEFAIKVPNFFIQIRNLAPGANVRCNNGHCTGADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU04</accession>
    <name>T1GU04_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU04"/>
    <dbReference type="STRING" id="36166.T1GU04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007203-RA">
        <property type="protein sequence ID" value="MESCA007203-PA"/>
        <property type="gene ID" value="MESCA007203"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_554660_0_0_1"/>
    <dbReference type="OMA" id="CRRYYQY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF275">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="7">
        <location>
            <begin position="324"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="492" mass="56469" checksum="6B416EA2EF7701D6" modified="2013-10-16" version="1">MSDHGFKDFTSLRELNVSGTRLNSLKQSWFLVRSAMESLDISHNYLNKVFRNSFINLSNLKHLNLSHNDIDSVEINAFADISFLNNLDLSFNDIKELSFGECTRLKSINLGNNLINVIPQYAFKGMPNLEKISITYNQIIILDLSGNSLSELKSGMFFGLRLIRKIYLNNNDIKQIEANSFNGLLNLDTLNLSGNNLDLLSKDIFAPSLPKLRKLLLSTNNIKTISSDLFANTPYLEYISLSHNELKQLEKNTFKILRRLRKLHIGHNIIENLDESMMNDFEGLSEFLLDHNKITFIPDCKHQFPNLIKTAIEGNPWQCPCLDEVIKFLEQKKVQYRKKFYFEGTKPVCVITSSKTCVKDFEFTKNEKVIDTYESITSGDEDDENIADSDLIYTETLMQRGIENYEGKEICILGGGDGALLYELLKEAPKHVVMIEIDVDVMDACNKYMNSICGDVLEKRKGHNYEIIVGDCMEYMKMFQQQGRKFDYVFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU98</accession>
    <name>T1GU98_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007308-RA">
        <property type="protein sequence ID" value="MESCA007308-PA"/>
        <property type="gene ID" value="MESCA007308"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457349_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007308-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10378" checksum="F91E0F0DBEACD24F" modified="2013-10-16" version="1">MLERSVKAPMCVLERRIDNQNLVESDRIWKICPMGRTKKLFGLDVYNKRANLAGLHPSGGRYKYAYRNVTSETKDYEKQQHTSETKQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV59</accession>
    <name>T1GV59_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007643-RA">
        <property type="protein sequence ID" value="MESCA007643-PA"/>
        <property type="gene ID" value="MESCA007643"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973003_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007643-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="13506" checksum="20D06AADF65225CB" modified="2013-10-16" version="1">MEAAEVEVALMSFPIHNESEELMILGSPMSESLATDTSELGDSFMDPIMDTQLDADFGSLDFDNTYRYTDVSTPCSSLSPASSGPLQSPASYSLAPEPPMSSPSPPPSAKELTDFLLISVCSSKTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVK8</accession>
    <name>T1GVK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">15-oxoprostaglandin 13-reductase</fullName>
            <ecNumber evidence="2">1.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">15-oxoprostaglandin 13-reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133374"/>
            <dbReference type="ChEBI" id="CHEBI:133409"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:50590"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15-oxoprostaglandin E1 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133408"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:50586"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15-oxoprostaglandin F1alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:79072"/>
            <dbReference type="ChEBI" id="CHEBI:133411"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:50594"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADP-dependent oxidoreductase L4BD family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02024432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVK8"/>
    <dbReference type="STRING" id="36166.T1GVK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007817-RA">
        <property type="protein sequence ID" value="MESCA007817-PA"/>
        <property type="gene ID" value="MESCA007817"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_29_3_1"/>
    <dbReference type="OMA" id="YPIKNIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047522">
        <property type="term" value="F:15-oxoprostaglandin 13-reductase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006693">
        <property type="term" value="P:prostaglandin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="8">
        <location>
            <begin position="110"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007817-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="285" mass="31539" checksum="5279394E2D2462AC" modified="2013-10-16" version="1">MEESKVGSVILGDQIARVLKSANYKYPEGSIVFGKFGWTTHTILKPHDEGWNHQNPLHSIYLLPDLKKKPISYALGVLGIPGNTAYFGLLDILKPKPRETVAVSAAAGCVGSLVGQIAKIKECKVVGFTSSQEKCEYLVRVLGFDYAFNYNSSDLSQQLKTTLPNGIDCYFDSVGGKLTNTILEHMNHFGRIAVGGCISLYNNCAESAIAPITHPKLLSKELKVEGFNVHRWNKSWADGIQQNLDWLREGKLIYNETISLGFENIPKAFIDMMNDENIGESIVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVT1</accession>
    <name>T1GVT1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVT1"/>
    <dbReference type="STRING" id="36166.T1GVT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007897-RA">
        <property type="protein sequence ID" value="MESCA007897-PA"/>
        <property type="gene ID" value="MESCA007897"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_352447_0_0_1"/>
    <dbReference type="OMA" id="DAEFICY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF19">
        <property type="entry name" value="MITOCHONDRIAL AMIDOXIME REDUCING COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="365"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="652"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007897-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="798" mass="90374" checksum="63C6259B278CB8AC" modified="2013-10-16" version="1">MVSDSLKLKMIVGLGIGASALAASTAAYYKYRNKKLQTPPSKWKNIGTIEDIYIFPIKSCAPIKLDAAHCDNLGIIEQKVRDRALMLIDVNGKVVTARTYNHMWKIQPKILANGTLLITAPGMDDIEFDHLTVGAQSNGDIKANIFGTDVYAMPCGKNFDQWFSKYILQKDEGIRLVYYPFPMPTKKIAEPIFDNGIFLTSDTVNSVKLSVLLGIGASLSAALAASVYFTQKRRKLQTPPNSWKEVGHIQEIHFFPIKSCAPIVLESVNCTNLGILEQGFVDRALMLVDKTNKMITARTYNHMMKIQPKIISHGKLLLSAPGMEDIELDVFTTFGEDLDNWFSRFILKKENGLRLAHYPHPVATRPIVEPIFDNGVFLKSDTGTFFDVSSYMLIHENSVNELNTRLEEKVEPLNFRGNFVIKTNQAPYAEDHWNWVRIGEEAIFRSLAPCYRCIFPNINPHTAERNKDNEPLKTLKSYRSWGNYKAPRFGIQMGLRKEGVLKKGDPVYVEGLEGVPNKVTEQPRVVKSCAPIVLDSATCTNLGVSEQGFLDRGIMIVDRNNKFITGRTFNNLMQIKPRIISEGKLLLSAPGMEDMELDFNEKESEDIKVEIWNAKVWVKTISGHVDRWFSKFLLNEDVGLRLVYYPYDKATRPILKPAVFNNGIFLKSDTGTLFDVSSYLLTHKNSLDELNSRLSEKVGSLNFRGNFLVKTTQAPFGEDSWEWIQIGQAVFRSLSPCYRCIFPNINPQSSERNKDNEPMKTLKSYRSWGISQFPRFGIQMGIRKEGMLKKGDAVYIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWA8</accession>
    <name>T1GWA8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase M13 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWA8"/>
    <dbReference type="STRING" id="36166.T1GWA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008081-RA">
        <property type="protein sequence ID" value="MESCA008081-PA"/>
        <property type="gene ID" value="MESCA008081"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725102_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF238">
        <property type="entry name" value="FI07649P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008081-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8069" checksum="4A5B106BB65E54BE" modified="2013-10-16" version="1">MRPEAIKNKLKTAVHSPGKFRVIGTLSNSVDFAREFSCPIGCPMNPTHKCSIFEYTLSQCKRYELGLLGYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWY0</accession>
    <name>T1GWY0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWY0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008315-RA">
        <property type="protein sequence ID" value="MESCA008315-PA"/>
        <property type="gene ID" value="MESCA008315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515219_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="10164" checksum="6B865D44A44A4283" modified="2013-10-16" version="1">MDNEIKDYVDTRSITRFKESTEDKWRRHRRPSRDPMFGVGFAVRGKGRYFVTRRTTINERLCMLRTFTRELYACILSTIPPTAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX66</accession>
    <name>T1GX66_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008408-RA">
        <property type="protein sequence ID" value="MESCA008408-PA"/>
        <property type="gene ID" value="MESCA008408"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009652_0_0_1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008408-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14204" checksum="5200FAD73AF8B641" modified="2013-10-16" version="1">KQSEIKEWKDYNSAIESTKDTARLRKILSKIHSSLAFVKNLHGSWAESSKERNEILLETHFPGCQQIATEESDCGSKLITYEKAINSFDPFNSADYIGNTQHAYVKGLPGDFRNFFEENEEIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXE1</accession>
    <name>T1GXE1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
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        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="MESCA008488"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008488-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10550" checksum="0A20285900258E3C" modified="2013-10-16" version="1">FSRSIQDNHKFTVTPSLEGFVHFFWNSIAHNHIAQIYWEIKIFDLPIIRKQSHIIVGQHKQYPVSLVEDYVVGFDICYNKFKQNVEEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXF0</accession>
    <name>T1GXF0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
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        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA008498"/>
    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="16831" checksum="6DC75AEABE93123E" modified="2013-10-16" version="1">MLHWAIFPNRSESVNRVVAVNVEIGDSSSLSESESLESEEEPVFLVLFSPSIFIRRSFLCLPLAFSPGGGNINFSSTCIIECHLKVPDPVAQMIPVLCARQAITAVNLAHYRVLVAVLIHLQSHLKGQLVVLSIGMINLAFGHLRVQLFAPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYB2</accession>
    <name>T1GYB2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149577">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA008836-RA">
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        <property type="gene ID" value="MESCA008836"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998254_0_0_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="76"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008836-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="13565" checksum="222FE83DEE8D941A" modified="2013-10-16" version="1">MSPDCCPQILPHKIATVPIITQPKVLKNKSESRVNTFKDHFKSYPTVANANQLIQMTTSLIENFKNDNLLSQSIDGTSSFSSRTSCNSSVESTPRTMTPSLYSDQNQHSGYSAGGPLAAASKMVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYL0</accession>
    <name>T1GYL0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <dbReference type="EnsemblMetazoa" id="MESCA008942-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="16727" checksum="924B0CA11B14460D" modified="2013-10-16" version="1">MDWKKLTVGIVVPTYGVISGKKSINLHGFIGVLKVDLAKRGGRFSTLHLHYFGTPGIDFFGGMHYFRFPSLISSPKNLATPWGLVQRSLVVCRPAHSIEENPVFENTNGNLLPIKQNSHSHESNFLKFMKLELLCSKNLSFILYSSSFD</sequence>
</entry>
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    <accession>T1GZD6</accession>
    <name>T1GZD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="synonym">Phora scalaris</name>
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        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="8098" checksum="3EA97033E20ADB41" modified="2013-10-16" version="1">MKPKRYGKQHTIKTSNSPLKPKVKRGESVSAFGKRICEVAYQYTSKAHNVMPNDFNEDQELRQNRRNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H061</accession>
    <name>T1H061_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02169960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H061"/>
    <dbReference type="STRING" id="36166.T1H061"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009529-RA">
        <property type="protein sequence ID" value="MESCA009529-PA"/>
        <property type="gene ID" value="MESCA009529"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174428_0_0_1"/>
    <dbReference type="OMA" id="ICHCIED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009529-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="10556" checksum="2D87779BE47729AC" modified="2013-10-16" version="1">MTFLKKYAYSATGFTLFVAALIVQWAILIKGFIKMEDGLIRFNQLGDLIDADIAAAVPLISMGALLGRTTPIQLLFMGIIEVALFAANEYVALDILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H086</accession>
    <name>T1H086_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H086"/>
    <dbReference type="STRING" id="36166.T1H086"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009559-RA">
        <property type="protein sequence ID" value="MESCA009559-PA"/>
        <property type="gene ID" value="MESCA009559"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444391_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21460" checksum="70BDCAA881A79716" modified="2013-10-16" version="1">MNTEKIEKIRVALAKFFFACNISFRSVESRQLAEFIELLNPDFLPYIPNRRNLSNELLDTVYEECIKKDKKQIREYSNMLVDGWTHSVTQTEWNTSLIHNADGTSAFVLAEELDIKTAIETSKSVGDAINKAKDLYGTEVYCLETDNAATMVSMGDNLKHKVWHIRCNAHTANLLVQDIIEKDLEGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Y6</accession>
    <name>T1H0Y6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Y6"/>
    <dbReference type="STRING" id="36166.T1H0Y6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009833-RA">
        <property type="protein sequence ID" value="MESCA009833-PA"/>
        <property type="gene ID" value="MESCA009833"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433193_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009833-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10184" checksum="6FEB70FC55589AB9" modified="2013-10-16" version="1">MAKKKNEEYADSGEDGEYQEEPNFEDPDGFVDDINDEDLLGDLLKQKPCETDGVENVVVVDNIPKVGPTRQDKLKTVIFKLFSTFGEIVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H193</accession>
    <name>T1H193_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. PYROXD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02162574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02162575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H193"/>
    <dbReference type="STRING" id="36166.T1H193"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009947-RA">
        <property type="protein sequence ID" value="MESCA009947-PA"/>
        <property type="gene ID" value="MESCA009947"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026335_0_0_1"/>
    <dbReference type="OMA" id="MCENLIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041575">
        <property type="entry name" value="Rubredoxin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF2">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18267">
        <property type="entry name" value="Rubredoxin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="10"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="236"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-rubredoxin oxidoreductase C-terminal" evidence="8">
        <location>
            <begin position="376"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009947-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="463" mass="52048" checksum="46F0B36505EBFF13" modified="2013-10-16" version="1">MEKETFLETKFLIVGGGIAGVSCAENVSFLCPDEKIILITESYLIKTVTNLRPLGKMVQSFDIKEEHIQTSSINQKVTVIEDLVTEIISRENKILTKSGKSIHYKFLCLCTGAQPKLIEQGNPYVIGIRDAESVLKFQEKKTVVVGNGGIASEIVYELDNIDIHWVIKDNYISSTFVDPGAAQFFKKRLEGSSDKPKKTIVKRMTYAKEDVLSGIQKGAALGPDWHRKIDISGYQSNHSLNIHYNSEVNDVHLTTNEDFPLRVTLSNGEIIYCDFLVSATGVEPNIKSILSDNLFDISQDGGIKVNEMMETNISNIYAAGDVCSADWKWASNWFQMRLWTQARQMGAMAGKSMAAKFKDERIYQDFCFELFTHVTNLFGYQVVLLGNFNGQGMENGYEILLRCTPEIEYIKFVIKEGRLKGAILIGETGLEETCENLILNEIDLTPFADDILNPNIDIEDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1B9</accession>
    <name>T1H1B9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1B9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009978-RA">
        <property type="protein sequence ID" value="MESCA009978-PA"/>
        <property type="gene ID" value="MESCA009978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239664_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12105" checksum="22B8CD3B17D8FF35" modified="2013-10-16" version="1">MLIGFTCGALREFENWAKFGKFWTKGCSFGHDTIKISNIGNIYLFGKETIDKHLFLERDNPINTSEKREFGVGFAVRGKARQYVTRWVDSSNSMYRPSEARVSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1R9</accession>
    <name>T1H1R9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02088280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02088281">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1R9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010137-RA">
        <property type="protein sequence ID" value="MESCA010137-PA"/>
        <property type="gene ID" value="MESCA010137"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3093468_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010137-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="5169" checksum="FC8908CA2613CD36" modified="2013-10-16" version="1">MLLPSSGLIIGSNGANTVGLCRQQQTPPPPNLISGLGPIGHHSTPPPVSGAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1X4</accession>
    <name>T1H1X4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1X4"/>
    <dbReference type="STRING" id="36166.T1H1X4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010198-RA">
        <property type="protein sequence ID" value="MESCA010198-PA"/>
        <property type="gene ID" value="MESCA010198"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052004_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029397">
        <property type="entry name" value="Tube_Death"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14786">
        <property type="entry name" value="Death_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010198-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="265" mass="29661" checksum="C6B8300CAE89A284" modified="2013-10-16" version="1">MYSRNDELRKVDSKHLRRVYSALDSLDSWQVLMGRIPKNLVSKTFTPRYNKDHVKIITEASKHRSPTEVLFDEWGTSGRIRPTVETLLDLLIEINMFRAADIVAKDLLGDKNVKKEVNIKSSSNINNDNDLSQNNIPDLSALNVEHERSSDNEIISDNIPILSELDVICNDEGYNFPDLSVLQGSSKEVILNLPEISALQNSIFNGTQIPELSALGNKNSSENLPEISALQNQVIPENLKSLNLPEISALKSVPECFLPELSGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Z3</accession>
    <name>T1H1Z3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Z3"/>
    <dbReference type="STRING" id="36166.T1H1Z3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010218-RA">
        <property type="protein sequence ID" value="MESCA010218-PA"/>
        <property type="gene ID" value="MESCA010218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087553_0_0_1"/>
    <dbReference type="OMA" id="GMFQASD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF1">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE EPSILON SUBUNIT HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="33"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13728" checksum="1A5F6B02394047AC" modified="2013-10-16" version="1">MLNPLEYHQVKGEKEKKDMLVIWKILIHLVDEFDILQIVGEGWFGKILLVEHRASGTEMVLKAVPKPYVSLRDFYREFHFGLHLGIHRNIVTTYDVAFETAGFYVFTQEYAPLVLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H279</accession>
    <name>T1H279_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H279"/>
    <dbReference type="STRING" id="36166.T1H279"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010315-RA">
        <property type="protein sequence ID" value="MESCA010315-PA"/>
        <property type="gene ID" value="MESCA010315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925081_0_0_1"/>
    <dbReference type="OMA" id="CDIKVGQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6881" checksum="1B7C33C747F5FB94" modified="2013-10-16" version="1">MSKATSIKEALKRWEEKTHENSTEAKVIDLQFQWPPIEKLDASLSQLVNCDIKVGQGEPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2E6</accession>
    <name>T1H2E6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2E6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010391-RA">
        <property type="protein sequence ID" value="MESCA010391-PA"/>
        <property type="gene ID" value="MESCA010391"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388732_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010391-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10745" checksum="1F6EE166D9066F45" modified="2013-10-16" version="1">MHTYQNKGGCKAEEVEKRNSAKSKFFRDFNSIGTEIRRGNSIKTSINNGKITPQHSPPVRMSYLFWTFATCECPITTGRSPIYCDDLLLLDIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H316</accession>
    <name>T1H316_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H316"/>
    <dbReference type="STRING" id="36166.T1H316"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010626-RA">
        <property type="protein sequence ID" value="MESCA010626-PA"/>
        <property type="gene ID" value="MESCA010626"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1995211_0_0_1"/>
    <dbReference type="OMA" id="CAWWVMV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF2">
        <property type="entry name" value="PROTEIN RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010626-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14139" checksum="CEA437CCEA93F586" modified="2013-10-16" version="1">MINYHSMRYGLLRLGAILLFASCDFGFAIYARYAALDNPLLPSVSYVAHLTGAFAGLTIGLLVLKNFEQKLHEQLLWWIALGIYAAFTIFAVTFNIFNSVEMRRIKNEYPNLVGAEDEMVFHDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H324</accession>
    <name>T1H324_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED18</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02126420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H324"/>
    <dbReference type="STRING" id="36166.T1H324"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010635-RA">
        <property type="protein sequence ID" value="MESCA010635-PA"/>
        <property type="gene ID" value="MESCA010635"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084570_0_0_1"/>
    <dbReference type="OMA" id="ARGYMFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010635-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="212" mass="23696" checksum="92DF4152C3FBAED0" modified="2013-10-16" version="1">MSGVPSTQDLLSQALNSKIIPNQEYLLQGSILDSASDHLFQRLKGLCDNVESGPQLFNDHELCLSLRAPNQPQPLMLRVRRSLDVDAPYQLRYIGQPEVGDRTRPTLVRSSIDVGCTSTVIDFLTELGCKIDFEYFSKGYMFRKGRMKITMSKILKVGKGDEALSQSQLVELSVLAPTGQDAIGEEMKSFAEQLKPLVQLEKIDYKRLGTMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3B7</accession>
    <name>T1H3B7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3B7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010739-RA">
        <property type="protein sequence ID" value="MESCA010739-PA"/>
        <property type="gene ID" value="MESCA010739"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533979_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010739-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9873" checksum="AEE18E816985580F" modified="2013-10-16" version="1">LVLGIEIWKAFTIDRLIAICNWINSLVAYCNLCSSSHFYASFRNKPLHHHSNCLCYLYILYLCGEFLKITLLILIDMRLQKSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3F5</accession>
    <name>T1H3F5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3F5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010781-RA">
        <property type="protein sequence ID" value="MESCA010781-PA"/>
        <property type="gene ID" value="MESCA010781"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640963_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010781-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8815" checksum="AD5B9154EA81E58E" modified="2013-10-16" version="1">MVCSSFEGSKNKSVSRSSRSFHVDVFFVILFLVRDGIFFFNSCYRGHCTNCNESARISKNSQSKYIMVACIRKTTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3N0</accession>
    <name>T1H3N0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Maestro-like HEAT-repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3N0"/>
    <dbReference type="STRING" id="36166.T1H3N0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010861-RA">
        <property type="protein sequence ID" value="MESCA010861-PA"/>
        <property type="gene ID" value="MESCA010861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777505_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048465">
        <property type="entry name" value="Maestro-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045206">
        <property type="entry name" value="Maestro_heat-like_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120:SF0">
        <property type="entry name" value="MAESTRO HEAT-LIKE REPEAT FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120">
        <property type="entry name" value="MAESTRO-RELATED HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21047">
        <property type="entry name" value="HEAT_Maestro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Maestro-like HEAT-repeats" evidence="1">
        <location>
            <begin position="156"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="357" mass="39714" checksum="BBCBA4E7B765510C" modified="2013-10-16" version="1">IVPRCIDTNATVRQISVDILQKTLEISCIYDTLTIAGSDDDWVKQLEHIKEFIISDDPKEIDKMAGEIAKIIALRMSNFQYLQFCKTLLGGLQDHELTSSIGSAGGGEIFSIFSFVKLFLEDCKIMNLHLLLDLLGGGCIPELVNESFSAIKYIDVSRARAKSGVLKSIVALTKHHPKLVCNEMLSQSLPFEENVVKYWNTVCLNEELTAQIIDNFLAILMASCLYEPSNDNAGDRQKIASVQPFAIFCAMREIFPSKEIKIKKFPELFAMLLTSLATYTNLAPPMLSVKSTSPTSNKTTSTSKSKFGFIPNKDSIKMNPCQVVLDTFLAFLNNLEMEQLFNVLTVCPSLAKSADLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3R8</accession>
    <name>T1H3R8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Calcium pump</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02089720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3R8"/>
    <dbReference type="STRING" id="36166.T1H3R8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010904-RA">
        <property type="protein sequence ID" value="MESCA010904-PA"/>
        <property type="gene ID" value="MESCA010904"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_10_7_1"/>
    <dbReference type="OMA" id="TSMDQYG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000003">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000005">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="291"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010904-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="343" mass="37124" checksum="770F91A509507293" modified="2013-10-16" version="1">MSSYCTPLKSSKLGNGPKLFVKGAPEGVLDRCTHARVNGQKVPLTSTLKNRIFQLTAQYGCGRDTLRCLALATADSPMRPDEMDLGDSTKFYQYEVNLTFVGVVGMLDPPRKEVFDSIVRCRAAGIRVIVITGDNKATAEAICRRIGVFGEDEDTTGKSYSGREFDDLSPAEQKAACARSRLFSRVEPAHKSKIVEFLQSMNEISAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLADDNFSSIVSAVEEGRAIYNNMKQFIRYLISSNIGEVVSIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRKADEGLISGWLFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3W7</accession>
    <name>T1H3W7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389127">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3W7"/>
    <dbReference type="STRING" id="36166.T1H3W7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010964-RA">
        <property type="protein sequence ID" value="MESCA010964-PA"/>
        <property type="gene ID" value="MESCA010964"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2284466_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010964-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11888" checksum="3C661BF4241044C7" modified="2013-10-16" version="1">MFNEWNHNIFCPKEEKTVIVITSAFLSVGCLKFEEKFSWVNVEYNWPSEEVHQEALASKEYIPEHNVIWRNGVASGLNYIELPSNEMSPKLNPYPSWNANLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H420</accession>
    <name>T1H420_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 181</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H420"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011019-RA">
        <property type="protein sequence ID" value="MESCA011019-PA"/>
        <property type="gene ID" value="MESCA011019"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248069_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011019-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="25633" checksum="EAB383C808512E93" modified="2013-10-16" version="1">MEQRNRKVKFNEKLEEYEPGTTVDDFFDIIDCDETTPKLKFQDIKEEEATIQEVEELLLGEIVESVLPKIDSDIEIDEISEEISEMSFGSKVRPKSASAIRDEPVVSSDENEKYIESCIENQEEEITDDNEDILSTLVASYVPENIPIPARKPQKEVQSKTQKSKTKQTKYGLTKEQLDAREKQIEIKKAKYMEKNLNATKAQIDKKCENEAAFTIWLKNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H468</accession>
    <name>T1H468_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EB1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02131600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H468"/>
    <dbReference type="STRING" id="36166.T1H468"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011071-RA">
        <property type="protein sequence ID" value="MESCA011071-PA"/>
        <property type="gene ID" value="MESCA011071"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840131_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EB1 C-terminal" evidence="7">
        <location>
            <begin position="94"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011071-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16310" checksum="DBE76F55B39D2EEF" modified="2013-10-16" version="1">MLTVESPHYQNNWFLSSITDQLLQGGFTKIKESKNVPIDRLIKGKFQDNFEFLQWFKKFYDSSEGQSSTSLDVNNNKDNCKTKPRPITAPKETTNEGSLNALKDLEEEKSKLTEQMEAIEHERNFYYGKLQMIEMLCNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4C3</accession>
    <name>T1H4C3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4C3"/>
    <dbReference type="STRING" id="36166.T1H4C3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011132-RA">
        <property type="protein sequence ID" value="MESCA011132-PA"/>
        <property type="gene ID" value="MESCA011132"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415762_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040259">
        <property type="entry name" value="Mesogenin/MesP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937">
        <property type="entry name" value="IP14615P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937:SF3">
        <property type="entry name" value="IP14615P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011132-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10598" checksum="04EA0F17EF4521A5" modified="2013-10-16" version="1">MRDMNVAFDLLRSKLPVKKPSGKKFSKIESLRMAITYIKHLQLQLGTPVEGEEVAHYYNLRSSSQISGNSTPNWALNLSKNRNYSRGEKEWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4H0</accession>
    <name>T1H4H0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4H0"/>
    <dbReference type="STRING" id="36166.T1H4H0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011179-RA">
        <property type="protein sequence ID" value="MESCA011179-PA"/>
        <property type="gene ID" value="MESCA011179"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418435_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031434">
        <property type="term" value="F:mitogen-activated protein kinase kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF13">
        <property type="entry name" value="TRIBBLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="22167" checksum="3B354F996802426F" modified="2013-10-16" version="1">TQIQYESLEGSIILNNPEDDTLYEKIGCPLYTAPELLTPNSTFQGKPADMWSLGVILFTMLLGQYPFNEKNGHSLFINIRNHNIVIPNALSGKVRCLIRSLLRRNPEERLIADDIFYHPWLKSAGKVRCLIRSLLRRNPEERLIADDIFYHPWLKSAPKSVQFLEQKYDVDMDDHCVPEMPSMECDDDVEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4X7</accession>
    <name>T1H4X7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4X7"/>
    <dbReference type="STRING" id="36166.T1H4X7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011348-RA">
        <property type="protein sequence ID" value="MESCA011348-PA"/>
        <property type="gene ID" value="MESCA011348"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_10_1_1"/>
    <dbReference type="OMA" id="VYERMKW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="93"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="189"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="293" mass="33142" checksum="928682A168774273" modified="2013-10-16" version="1">MTIDCTRLSWCQKIGGGDRLILQSTDGPALSKEVPYSTKGAVDFLKQIYIREGFFALWRGNSATMARIIPYAAIQFTAHEQWRKILKVDQDGDTKGRRFVAGSLAGITSQSLTYPLDLARARMAVTDRYTGYKTLRQVFIKIWVKEGPKTLYRGYSATILGVIPYAGTSFFTYETLKKEYYDLTGNKSPNTLITLACGAAAGIAGQTSSYPLDIVRRRMQTMGIHKNSKDSYPNILKTLQKIYREEGIKNGFFKGLSMNWIKGPIAVGISFSTYDSIKQFLRELMYLRRTRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5J6</accession>
    <name>T1H5J6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
            <location evidence="8">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5J6"/>
    <dbReference type="STRING" id="36166.T1H5J6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011574-RA">
        <property type="protein sequence ID" value="MESCA011574-PA"/>
        <property type="gene ID" value="MESCA011574"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071690_3_0_1"/>
    <dbReference type="OMA" id="LEWAENY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000002">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="10">
        <location>
            <begin position="168"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011574-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="284" mass="32942" checksum="5D2947296FB3D22A" modified="2013-10-16" version="1">MGKKDPPPPPEPPETIKFDPRFPNQNQTRHCYQSYIDYHSCINLRGEGYQPFSLTKTDRKGLAWKQLIIDDVKRCIEKYPNVFIFSVENMRNNLLKDLRQETKKNSRFFFGKNRVMQIGLGRNEAEEIEPELHKLSERLTGQCGLLFTERSKEEILDWAKDYWAVEYARGGFEATETVILPEGPMEEFSHSIEPHLRKLGMPTKLEKGIVTLYKSYTVCEKGKVLTPEQARILKLIAKPMAKFKLTIKCSWSKTGGFESYVNEDSGNEEGEDEDEEDVEMEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H600</accession>
    <name>T1H600_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycoside hydrolase family 2 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H600"/>
    <dbReference type="STRING" id="36166.T1H600"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012133-RA">
        <property type="protein sequence ID" value="MESCA012133-PA"/>
        <property type="gene ID" value="MESCA012133"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168226_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019391">
        <property type="term" value="P:glucuronoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066:SF67">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="3">
        <location>
            <begin position="10"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012133-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="10089" checksum="DE6D265E9B0F20C4" modified="2013-10-16" version="1">KIVYSSHPMYEEFQTTLCKHFKAFDILRKTGWFIGEFVWNFADFKTAQTITRVGGNKKGVFTRSRQPKAVAHLLRKRYFAIGQEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6B4</accession>
    <name>T1H6B4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6B4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012247-RA">
        <property type="protein sequence ID" value="MESCA012247-PA"/>
        <property type="gene ID" value="MESCA012247"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140399_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF1">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012247-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="244" mass="27839" checksum="863C8C23CD902F0F" modified="2013-10-16" version="1">KSSNSSFEDLSNLNEAETAKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFEEYGDLKSFKIRKEYGNRKRERANFWYNRNEFNTAIQIYRRALEYLDHKDGDPDNVDGELDITNENLQSLLDDRLIVYNNLAMAQIKVSAYDAALASVENVLRCQPNNSKALFRKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6K5</accession>
    <name>T1H6K5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="3">4.2.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hydroxymethylbilane hydrolyase [cyclizing]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Uroporphyrinogen-III cosynthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>hydroxymethylbilane = uroporphyrinogen III + H2O</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="T1H6K5"/>
    <dbReference type="STRING" id="36166.T1H6K5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012338-RA">
        <property type="protein sequence ID" value="MESCA012338-PA"/>
        <property type="gene ID" value="MESCA012338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051874_1_0_1"/>
    <dbReference type="OMA" id="IHGADTG"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006785">
        <property type="term" value="P:heme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10090:FF:000003">
        <property type="entry name" value="uroporphyrinogen-III synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390">
        <property type="entry name" value="UROPORPHYRINOGEN III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390:SF0">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="27391" checksum="9382D6C2444EFB51" modified="2013-10-16" version="1">TVILLKSETESSDVYGRLLEANNFKAIFVPTLGFGFKKLDELKKKLEHPEKYAGIIFTSPRSVDAVRGALGEDPNARMLCNYSVGEVTHNLVMSTLNQLFTKGKETGNANNLSEFIKDGFDGNKSLPFLFPCGNLRTDTLITKLSECGFQLDAFEVYETICHPDLGENLKKALEEENVEFMAFFSPSGVNCTYEYFQKNNLSFNGKKLIAIGPSTRKCIEGKGLEVYSVAEKPTVDYLIKVLINPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H706</accession>
    <name>T1H706_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">eEF-1B gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H706"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012488-RA">
        <property type="protein sequence ID" value="MESCA012488-PA"/>
        <property type="gene ID" value="MESCA012488"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_3_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012488-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="25794" checksum="E6815A06FFF5FD4D" modified="2013-10-16" version="1">TLYTYPENFRAYKALIAAQFSGAKVKVAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIMPQERRRTAKKDVTRVLEVLNKHFLGNTFLVGERITLADVVVFSNLLHLYQYVLEPSVRAPFGNLNRWFNTILNQPQVLAVLKNFQICAKQLQFDPKKYAEFQAKSGAAPAQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H709</accession>
    <name>T1H709_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FXNA-like protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H709"/>
    <dbReference type="STRING" id="36166.T1H709"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012491-RA">
        <property type="protein sequence ID" value="MESCA012491-PA"/>
        <property type="gene ID" value="MESCA012491"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237726_0_0_1"/>
    <dbReference type="OMA" id="SHAFINQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000008">
        <property type="entry name" value="Endoplasmic reticulum metallopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M28" evidence="15">
        <location>
            <begin position="34"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012491-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22275" checksum="282C8672C480F44B" modified="2013-10-16" version="1">ESRQDLYDIDYDIQEASGSYVIFNMVNAYQSIQNVVVKFSAKDSNSTNYLLINSHYDTVAESAGAGDAGVMITTMLEVLRKISKSSNTLRHPIIFLFNGAEENPLQGSHAFINQHKWANNIKALINLDSAGSGGREILFQSGPNHPWLMKYYKRCAKHPYASTIGEELFQHNFSRYRFPSFRLRRDSWTGHGSHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7I8</accession>
    <name>T1H7I8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7I8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012725-RA">
        <property type="protein sequence ID" value="MESCA012725-PA"/>
        <property type="gene ID" value="MESCA012725"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2801351_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTF2" evidence="1">
        <location>
            <begin position="10"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012725-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7666" checksum="985CAF42B53EEA4C" modified="2013-10-16" version="1">MALNPQYADIGNGFVQQYYAIFDDPAQRANVVNFYSVSNFXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA80</accession>
    <name>T1GA80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA80"/>
    <dbReference type="STRING" id="36166.T1GA80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000130-RA">
        <property type="protein sequence ID" value="MESCA000130-PA"/>
        <property type="gene ID" value="MESCA000130"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798657_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000130-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16879" checksum="D4C3AF5DCF904252" modified="2013-10-16" version="1">MYREIKELTKSKKRKQPKEESTELKRSKKSDEENSGDEADQPTAEQLKESEKPENQPDIQTVRQKKKKKHQKNVEMSKESSEQREILKNSELIGGSSTKGFKTIWIRNKIIVKTIERNPNYPLQNQPRIKLNFSCNPEKHNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAT5</accession>
    <name>T1GAT5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391540">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000346-RA">
        <property type="protein sequence ID" value="MESCA000346-PA"/>
        <property type="gene ID" value="MESCA000346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1162309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577217" description="VM domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="25027" checksum="DF4DF3AE14CBAD8B" modified="2013-10-16" version="1">MIKFMVLAVLSVASVQCGIVPVASTFSAHRINHAIAHPVAAAPVAPAPVNFAVPQAVPFGGHYHGHVPVPAPVNFAAPAPVNFAAPAPVNFAANFAAPSPYYPHYHVPYNYPQYGFPPQQYAFPGAQNAPVPQGPQPMNDAAPASASPAPVNDAPQQQGPVSFSSQIFQMGPGALNGQNFNAPSPAPAQVDEQQAQAAPAQQAQADAAQQQPQQAQQQQQPQVVDHQQQAFASPFFSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAV0</accession>
    <name>T1GAV0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAV0"/>
    <dbReference type="STRING" id="36166.T1GAV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000365-RA">
        <property type="protein sequence ID" value="MESCA000365-PA"/>
        <property type="gene ID" value="MESCA000365"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_563347_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000010">
        <property type="entry name" value="Potassium voltage-gated channel subfamily B member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF254">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="304"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000365-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="485" mass="55750" checksum="41F0C10F0B3B1683" modified="2013-10-16" version="1">MVGERDRDREAVRWELGDISTPQYNNGGSQMVGGTGTLCGGQQVAYTQQQQQRLLQQQQQQQQQQQVAAQQQLLQQQVLQGNWTAESEQIRRNESEIDDQQFYDTTAGNIGWPRNNPNYGYIDNFDVGVVGVGANSYNVPQFVPVGSSSNYIIILIQHHWVLVLITTVLVKLRLLVQQLVSLVVRKSVMLHYLLLHRIHILQILQLPNNKCQCNHSNSFNNNSNHNNIGSCTIPRQQLQFYNINSSTNATTTVAPAATTVTTATSPTAGGCYCRYIIFTTDTFQIYASIPAPEPFMIAQAKQQSRVLINVGGVKHEVLWRTLERLPHTRLGRLRQCTTHEAITELCDDYSLVDNEFFFDRHPKSFSSILNFYRTGKLHIVDEMCVLAFSDDLEYWGVDELYLESCCQHKYHQRKENVYEEIRKEAESLRQRDEEEFGEGKCAEYQKYLWELLEKPNTSLAARVSIPFRGWVFFISKTLAVTWRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAW5</accession>
    <name>T1GAW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAW5"/>
    <dbReference type="STRING" id="36166.T1GAW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000380-RA">
        <property type="protein sequence ID" value="MESCA000380-PA"/>
        <property type="gene ID" value="MESCA000380"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_517093_0_0_1"/>
    <dbReference type="OMA" id="HVVYPPY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576773" evidence="2">
        <location>
            <begin position="25"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000380-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="527" mass="58294" checksum="61D478D4920906E5" modified="2013-10-16" version="1">MDYNYALLSQKLFVVLAFVAVASADVSHLLGGSSHQGYNYPAPQPSVLQPHHVSPPAPQPQQGYNYPKPQPQHIPPPQPQQQYLPPAPAPQPHIPAPQPHIPAPQPHIPAPQPHIPAPQPEYLPPAPQPHIPAPQPHIPAPQPHIPAPQPEYLPPAPAPQPHIPAPQPQEPGYHYNAPHRRFRYRNGTASAWIWIIIDFPQKLFLVLAFTAVASADVSHLLGGQSGYNYPKPQPQHISPPAPQPKGYDYPKPQPPQPKGYDYPKSQPPQPKGYDYPKPQPQEISHPAPQPKGYDYPKPQPQTPTTSIERIDYPKPQPPQPKPQPKGYDYTKPQPQPISPPAPKPEYLPPAPQPHIPVPQPEYLPPAPHIPAPQPEYLPLVSQPQEPGYHYNAPQRRFLLALVAVAAADVSHLLGGGSHPGYDYPKPQPSAPESQYIPPQPAPQPSIPEPQYLPPQPAPQPSIPEPQYLPPQPAPQPSIPEPQYLPPQPAPEPSIPEPQYLPPQPAPQPEEPGYHYDAPQYRLFRFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB22</accession>
    <name>T1GB22_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000442-RA">
        <property type="protein sequence ID" value="MESCA000442-PA"/>
        <property type="gene ID" value="MESCA000442"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690642_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000442-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8434" checksum="9AE6FEC35665B27A" modified="2013-10-16" version="1">MACPCPKKRRSRRPKKRACRKRPRPRRRACKSRPKRKKNPCKPKKKFCGMTPSQTVVAGAKAGIEWISSASLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBM5</accession>
    <name>T1GBM5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GBM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000661-RA">
        <property type="protein sequence ID" value="MESCA000661-PA"/>
        <property type="gene ID" value="MESCA000661"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1035435_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF4">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="160"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="269" mass="30237" checksum="A46940536407E285" modified="2013-10-16" version="1">MSEDYDTLLGFGMLSHRHAKALWKSCVEHHSFFRLKRPHRMTRFLNISLGSKFYYSGRTELQTVQECKQSSVVPKAFVRSPSKRLLNGPNDGTSSLNGSNKNPILTITKTSHRPHDNKVTSKQSDSLPRKAWEHSDNEEDGSENHTPIASICDSDNQLVTIKLIADEQGRYGFNVKGGIDLSLPIQVSKVVPNTAADNASPKICEGDEVVRINGKDVCNLRHEEVVEMIRSSTRDGLHLTVRPVHSIVGLMEEEEEPLYQYVPETDEMX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC48</accession>
    <name>T1GC48_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02172761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC48"/>
    <dbReference type="STRING" id="36166.T1GC48"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000846-RA">
        <property type="protein sequence ID" value="MESCA000846-PA"/>
        <property type="gene ID" value="MESCA000846"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_16_5_1"/>
    <dbReference type="OMA" id="FKTIWTL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000449">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000657">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="210"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="390"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000846-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="545" mass="61911" checksum="4525D40BE92D5DF5" modified="2013-10-16" version="1">MSGNSEVPIKRYRREDPKSSDEDDQDDDKYVPYISVKERKKNHLLKIGKIQMLPSENEKTQIKPVSEDESEEEANTWGRKYNISLLDQHTELKKIAESKKISEVEKQLKEEEKILESVAEKKALMGVSELAKGIQYEDSIKTSWKPPKYIADMSEEKYDRIREKLRILVEGDNIPPPIRSFIEMKFPEGILRGLKAKGITKPSPIQVQGLPAVLSGRDLIGISFTGSGKTLVFVLPIIMSYGLIICPSRELAKQTYDIIKFFCLQLMEHHNFPEIRHCLTIGGVPVSEAIEVINKGVHIMVATPGRLMDMLDKKIITLDVCRYLCMDEADRMIDMGFEEDVRTIFSYFRGQRQTLLFSATMPKKIQNFARSALVKPITINVGRAGSASLNVKQEVEYVKQEAKIVYLLECLQKTAPPVLIFAEKKQDVDSIHEYLLLKGVEAVAIHGGKDQEERIRAVESYREGKKDVLVATDVASKGLDFPTVRHVINYDMPDDIENYVHRIGRTGRSNTKGLSTTLVNKTTEQSVLLDLKHLLMEQNKVFLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCK9</accession>
    <name>T1GCK9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCK9"/>
    <dbReference type="STRING" id="36166.T1GCK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001024-RA">
        <property type="protein sequence ID" value="MESCA001024-PA"/>
        <property type="gene ID" value="MESCA001024"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047758_0_0_1"/>
    <dbReference type="OMA" id="RQFQAPI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001024-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="30404" checksum="3F51F02D6588E1D5" modified="2013-10-16" version="1">MKNHLSTVITAHVFISHTKQTASAASVHTVEDDSDMLMEPDETSHVSIVRSISPDIRRHFVDDAPQPPQGDNYPQAAQNYYPPFFRDNQPLGHSRPFLAPPIGKSIPSHGPRSFDPSILGSGDFGVIRGGTFYSEEDPAPSSHGEDYYASESNGHGRPQSEPYIQKYTYPEEQFANFRDFADINTPQDTGFSQFVIVYAAKNSTSSHPNPKNIFEQLELLDKEKAQEEKKMKKNQKSKSKAKLEKTKLEKRFKKQFGPKDLDYEPLLALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCM2</accession>
    <name>T1GCM2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001040-RA">
        <property type="protein sequence ID" value="MESCA001040-PA"/>
        <property type="gene ID" value="MESCA001040"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576597_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001040-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9161" checksum="7B7F4925875F8B6D" modified="2013-10-16" version="1">MTTASIKNFGAVKIRKGMQQKDSLSPLLFTTALKMYFLRGIDIFGNKLKKLRFANDIILISNKCCRTFATNGLPTGPFWEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCP9</accession>
    <name>T1GCP9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCP9"/>
    <dbReference type="STRING" id="36166.T1GCP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001069-RA">
        <property type="protein sequence ID" value="MESCA001069-PA"/>
        <property type="gene ID" value="MESCA001069"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023330_0_1_1"/>
    <dbReference type="OMA" id="MILPREK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19601">
        <property type="entry name" value="serpin42Da-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.580">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="31"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001069-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="347" mass="39759" checksum="15FFE0DA122DDF38" modified="2013-10-16" version="1">MLLKLKTNLGICNIYLTFHFLFVFGETNFERKIFNVLSKEKSSENLIFSPISIEVALALGYFATTGETRAEIGNLLDLHGNSSEVERLYDSVIDGAKDKYQMKILSKIWVDESVKVRPRFQRIAKKSFNSEVSNGDFTNSRKVAGEINKWVAQKTNNKIQNIISEESVNQDTLLVLLNAVYFKSEWEHLFKLQKRPGRFWNNGENMVEVLTMSSTRNYLYGYLKELNSFAIEIPYKNNELKMMMILPREKNGLQELEDKINNINFMDLMKTRMTYREVKVILPKFTISNEFDLVQVLSKIGVNKIFVNSGDFGGLFESPRTTKVSSARHSVFIDVNETGTEAAAATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDI8</accession>
    <name>T1GDI8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001376-RA">
        <property type="protein sequence ID" value="MESCA001376-PA"/>
        <property type="gene ID" value="MESCA001376"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932819_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="13856" checksum="4BC168E5B063D3DF" modified="2013-10-16" version="1">VTGIGVTPINNNLVSTEIGTTFSLPPVIEISSPSALSIPSFSDENSTDSDIIPMSPSNILRDVDTADPRWMDEYSMSSLLGHLDELANTSDTCNTPARSNESSSLSIISETSVDYVTKFAELAAAEENGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDQ5</accession>
    <name>T1GDQ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001448-RA">
        <property type="protein sequence ID" value="MESCA001448-PA"/>
        <property type="gene ID" value="MESCA001448"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017844_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001448-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14395" checksum="63F7A044A4B9E975" modified="2013-10-16" version="1">MKLKKKLTQNIQVVPDSLLKSNQSTAIANFLQRRPDVSLIESQMINHLMFPKLQRTPPLQHRATITQLQPAQQQHSLLSSIGYVQQQQQQQNYQFSRCPLCSRNYRSQAFLNEHMRKEHSVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDQ6</accession>
    <name>T1GDQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045170">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001449-RA">
        <property type="protein sequence ID" value="MESCA001449-PA"/>
        <property type="gene ID" value="MESCA001449"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852249_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001449-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="6120" checksum="0645300E6AA1A60D" modified="2013-10-16" version="1">MKKERIGVASGVGGVPLGGSFSGNNQSSSTTSATSTGGSGNTGTGSGNSGSGGWGGAYTCERCEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE82</accession>
    <name>T1GE82_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE82"/>
    <dbReference type="STRING" id="36166.T1GE82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001640-RA">
        <property type="protein sequence ID" value="MESCA001640-PA"/>
        <property type="gene ID" value="MESCA001640"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940510_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF8">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001640-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="15576" checksum="4E0F52B46CD4DC99" modified="2013-10-16" version="1">MEWVEIIEPKTKDVMFANLQTGEVVWEEPENVLIKRANNYQWWELYDVKTKRHYYYNTQTEETVWHKPSNYSEIIPLAKLQNYKHPDSKKKHNFVAVVNNRNDVVRSSSSNDVDFHHHHTGSSKNSFKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF47</accession>
    <name>T1GF47_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 11">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 11"/>
    <dbReference type="EMBL" id="CAQQ02065608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF47"/>
    <dbReference type="STRING" id="36166.T1GF47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001976-RA">
        <property type="protein sequence ID" value="MESCA001976-PA"/>
        <property type="gene ID" value="MESCA001976"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085513_0_0_1"/>
    <dbReference type="OMA" id="FKLNFIT"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.20:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014929">
        <property type="entry name" value="E2-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08825">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01181">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E2 binding" evidence="12">
        <location>
            <begin position="198"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001976-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="294" mass="32961" checksum="3AA26D9EAB657D12" modified="2013-10-16" version="1">MLVSLLNYEDGVLDPSSLIPMIDGGTEGFKGNARVILPGMTACIDCTLDLFPPQITYPLCTIANTPRLPEHCIEYVKIIQWPKESPFGDAALDGDDPQHVMWVYEKAQDRARQYNIAEVSYRLVQGVIKNIIPAVASTNAVIAAACVTEVFKIATQCYNPMNNYMVFNDLEGIYSYTYEAEKREDCLACSNIPREVNIEDPNSMTLEDLIKYLCENHDFQMKNPGITGVIDGKQKTLYMATVKSIEEKTRSNLTQSLFELGLKDGHELFVSDCTSPNTITLKLKYKGNEVEMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF81</accession>
    <name>T1GF81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF81"/>
    <dbReference type="STRING" id="36166.T1GF81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002013-RA">
        <property type="protein sequence ID" value="MESCA002013-PA"/>
        <property type="gene ID" value="MESCA002013"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087498_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF23">
        <property type="entry name" value="NEURAL-CADHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="76"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002013-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="27090" checksum="E94173D627038A52" modified="2013-10-16" version="1">MIDQTPHDDSKVFSLDPLTGSLTLIKALDYESQKEYILVIQAIDQSKNVTQRLHTTVTALILIQDVNDNRPVFVSPSKDTTITLNSGIQVGQLVTHVLAIDKDTDGDNGRIKYAILSGNDGGHFKMHERTGFVELIKPLPIMRSGEGDINVSFNKFEMVVSATDGGAPLPLEATLNLKFIIHGTSSSPPRFLQSYYYANISEDARPHIDGKSGVVSTRGHFNREHVDKYTIPIYVFETTTSASAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFP5</accession>
    <name>T1GFP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002192-RA">
        <property type="protein sequence ID" value="MESCA002192-PA"/>
        <property type="gene ID" value="MESCA002192"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608764_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588256" description="Cuticle protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002192-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8716" checksum="879CE0C3C32F2988" modified="2013-10-16" version="1">MKIIFVLALFLAAATAGYVPLAYSSYPVAHHHHHVAPIHHYSPIVAPVVKVVPVITPVVKVAPIAHYVPVAYQPWIKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG46</accession>
    <name>T1GG46_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02194788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG46"/>
    <dbReference type="STRING" id="36166.T1GG46"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002355-RA">
        <property type="protein sequence ID" value="MESCA002355-PA"/>
        <property type="gene ID" value="MESCA002355"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_0_3_1"/>
    <dbReference type="OMA" id="VECSEAI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577360" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="45"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002355-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="296" mass="33093" checksum="621722E8E9DB4A25" modified="2013-10-16" version="1">MMNIVCIFFLYIVSNISCTSYIGDLQRSLPVPPKCGLPTSIVKRSVGGGPTSVSHFPWTVFLIYDDLNIENDPEQGFCGGSLINSRYVLTAAHCIDPEPVSVLLGTTDLRNYVRYVDVDKVLANPRYYQLSDTVFDGLYDIGLIQLAVNVEFSNSIYPICLPFQIENYEPPNTNTTFLASGWGTTKYINYKYILTVVKVKLVDFEECFKVFSTQDQTLHSKVFCAGGTIGTDTCFKDSGSPVVREINGTWILEGIVSSGLDKSCGTLNPALYTKVLKYEKWIREQILNDFYFSGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG47</accession>
    <name>T1GG47_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02194789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG47"/>
    <dbReference type="STRING" id="36166.T1GG47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002356-RA">
        <property type="protein sequence ID" value="MESCA002356-PA"/>
        <property type="gene ID" value="MESCA002356"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_1"/>
    <dbReference type="OMA" id="YENWIVE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="78"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002356-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="316" mass="35298" checksum="56F8A6ACDFC94C4B" modified="2013-10-16" version="1">MNDYLLNNKVFCAGGDLGVDTCLGDSGSPVLRKIGNNWFIEGIVSSGLDNTCGTLNPVVYTNVSKYEDWIVENIVYGWQENLDYKDDPRLCGGSLINSKYVLSAAHCMDPDPVTIILGTTDFRNYTISSKVSKTLAHPNFKLVSESEFELLYDIALIKMEDSVKFSQKIYPICLPFQIENYEPPNRNTKFLVSGWGITKHIFENYILTAVEIKFYDFELCKKIFSMNYDFLNSKVFCAGGELGVDTCLGDSGSPVMRKVGNIWVIDGIVASGLDNFCGTLNPAVYTNVSKYENWIVEHIFYGFGSGESSKFLSLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG92</accession>
    <name>T1GG92_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02122287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002404-RA">
        <property type="protein sequence ID" value="MESCA002404-PA"/>
        <property type="gene ID" value="MESCA002404"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834065_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002404-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7886" checksum="48DA5B0AD09FEB8D" modified="2013-10-16" version="1">MYRHQISSISSQKQSYQYLTIFSLRLIRQLGDATHYRDFKDSFVEVQIVDEILKIPRIKNPGHQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH90</accession>
    <name>T1GH90_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH90"/>
    <dbReference type="STRING" id="36166.T1GH90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002780-RA">
        <property type="protein sequence ID" value="MESCA002780-PA"/>
        <property type="gene ID" value="MESCA002780"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534293_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01241">
        <property type="entry name" value="PH_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000404">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039026">
        <property type="entry name" value="PH_PKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF8">
        <property type="entry name" value="PROTEIN OPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="15"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002780-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20198" checksum="79E3633F4141FEB9" modified="2013-10-16" version="1">MMSTEEKSSSSTAANVVKQGWLYKRGEHIKTWRARYFILKDDGQLLGYRNNNDPANNVEPTNNFTVKRCQIMTLDKPKPFTFIIRGLQWTQIIERTFHVDTEEERQSWTEAIRNVANRIEPSESIPHGLSHEVDMNDVDMASIAEVELLQPFQLQVGTSTGKVSGKKKVNYYLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHH4</accession>
    <name>T1GHH4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002875-RA">
        <property type="protein sequence ID" value="MESCA002875-PA"/>
        <property type="gene ID" value="MESCA002875"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608752_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002875-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9047" checksum="9E28BE3E156F2F1D" modified="2013-10-16" version="1">MNQIEWSVVHLIVVNAGTRLQSGCSRRYLSSISLIEKYFVEKKRAFFSNSIEQFYVKTQEHHPKATSNTTKAIQISSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHJ9</accession>
    <name>T1GHJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02131848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHJ9"/>
    <dbReference type="STRING" id="36166.T1GHJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002903-RA">
        <property type="protein sequence ID" value="MESCA002903-PA"/>
        <property type="gene ID" value="MESCA002903"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141232_0_0_1"/>
    <dbReference type="OMA" id="PHMAFLH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577373" description="Cytochrome P450" evidence="3">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002903-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13085" checksum="746F51CF357387AD" modified="2013-10-16" version="1">MSPLLIGAIVILLLIKAYKWSTYRPEGCPPGPPRIPIFGNYLYLLLLNWNHLHKATSWLCKFYNTKIISLYLGPIMAYAVSDPDGIREVLNSSSYDGRPDLLLGRMRHPELKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL5</accession>
    <name>T1GHL5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL5"/>
    <dbReference type="STRING" id="36166.T1GHL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002922-RA">
        <property type="protein sequence ID" value="MESCA002922-PA"/>
        <property type="gene ID" value="MESCA002922"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328806_0_0_1"/>
    <dbReference type="OMA" id="ETNETEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="698"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="378"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="479"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="741"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="741"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002922-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="874" mass="100334" checksum="A9210F701D20A805" modified="2013-10-16" version="1">MFSNKKDCKLSVNKNIPSRYECTTTIEFAPYNFILYRVNGLSKKPLNQSFDINLLNVTVPPKVEFDSVRTNISSVEFNFSINESANYVYNSKNYLIFLVSLRTKLGILDQSEYQYEVKKAIGDIFSFKIENLPYPNYVYNVTFRCKMLLSPNSSDLYFSEPTHYTFMTNPTIPYRPPRTNLGAFEIIRPNLGNSNGNSILTIFWEKLEEYEFNGPNFEYDITVYKNNVPSDMKPSSKNHYSATYEIPRGAEYFTTDYRFEILSTNSKGNSPNSSRVNPNPIEDVWKVTNDFEANLTWSLQKDDDDLKSFTVYWCNARSRDEYSTCNDSIYFEEIPKHERVHTFNNTIVNYGIAMNYQKNSSGIVWNTCTSRKDKKLEQVKDFKHDPVQKREINLRWSTDCQFKSVINGYFLEYCLLNNISKNCTGKIHNVTLGNKNASHTVKHLKPYSRYKFGISMYRGQERAESSYLTIETKQDAPSCPQDLIVRNVTSSTVYLEWSKPEEMNGEFKFYKVFFNDKKGFNNSDYKSTTATNITLMNLDGNTNYLLYVVAHTVEDSEPSNVVSIKTLIGTPSSPKLADQPFKYQSSNIVANWYAPEKPNGLVDFYEISLGDRVSLVRNLSCTVDICDDPKGMDFKIIAVNVYESHNSTFEETFPNIYLNSLCAEDISYDISQRFTSNYKFYKSVALTNQLPCSRGTPILYVIILAVCIAISFVFFMSFYKIRKMQDIKVVLPPGLGYGSSPIKNNGTNTSQTQDEAESDQTIETNETECSTLTGHEYFKSLEFEEISEKPKTPFYLTNTAAPVIDNFTKQENNSGYIKIQPVSEYIQPNTCGYVTLPDLQKIKNESEYLPIETEESIPTPMMSGYVTPGGNFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHM1</accession>
    <name>T1GHM1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHM1"/>
    <dbReference type="STRING" id="36166.T1GHM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002928-RA">
        <property type="protein sequence ID" value="MESCA002928-PA"/>
        <property type="gene ID" value="MESCA002928"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834060_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF1">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002928-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7887" checksum="46BDE7B77422A441" modified="2013-10-16" version="1">MGFEFDVRVCDPCFKQLQFVERPSLATFHDAKHSIVFMDMDEERKRLLTVGQDRIIKIWDLSPIWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI18</accession>
    <name>T1GI18_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI18"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003085-RA">
        <property type="protein sequence ID" value="MESCA003085-PA"/>
        <property type="gene ID" value="MESCA003085"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3417468_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003085-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="26" mass="2942" checksum="839827F039768F6F" modified="2013-10-16" version="1">MLSTKKNPKPRAYIGNTTVNSCYQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIA5</accession>
    <name>T1GIA5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02095524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIA5"/>
    <dbReference type="STRING" id="36166.T1GIA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003174-RA">
        <property type="protein sequence ID" value="MESCA003174-PA"/>
        <property type="gene ID" value="MESCA003174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1246616_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003771">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="222" mass="23089" checksum="6B061AEF1CB63929" modified="2013-10-16" version="1">MLDFCLVLQETSELALGLSASMCGGSSGGSSANNSSSITAASTSAAADHGVLFTYSLKFREHQSHLLSLLPHDLQHFPHRPLDALDPSEHPSATAPMDIDFATAVATAANFQNMVAAAAGSSGVGGNSSLNISTNSGVVGSAPSSSAASSVMPSCSGNDPNSVVLCKFCGKSFPDVTSLITHLPVHTGDRPFKCEFCGKAFKLRHHMKDHCRVHTGKYIVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIP1</accession>
    <name>T1GIP1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIP1"/>
    <dbReference type="STRING" id="36166.T1GIP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003324-RA">
        <property type="protein sequence ID" value="MESCA003324-PA"/>
        <property type="gene ID" value="MESCA003324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_198661_0_0_1"/>
    <dbReference type="OMA" id="WLCLEAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7840" checksum="6AEBBA7F615B48F3" modified="2013-10-16" version="1">MTTKCRHIEKVEPSSIRLILEKSVNKCSICENGGPNLWLCLEANCLQIGCSEAHKDHSTMHFQVTRNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIW7</accession>
    <name>T1GIW7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02043791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIW7"/>
    <dbReference type="STRING" id="36166.T1GIW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003403-RA">
        <property type="protein sequence ID" value="MESCA003403-PA"/>
        <property type="gene ID" value="MESCA003403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415185_0_0_1"/>
    <dbReference type="OMA" id="GFWLYHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13858">
        <property type="entry name" value="CuRO_1_tcLCC2_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577407" evidence="5">
        <location>
            <begin position="19"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="65"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="196"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="428"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="601" mass="68790" checksum="8337F7827C7C4AE9" modified="2013-10-16" version="1">MNLLIVFSFLSLILITEASSKHTCDRVCSCDPSTFKLCKFNFRVHYSVMASEHCGDCRNNPEDCENENCIFGDGNRTQIIAINNIFPGPKIKVCEGDTIEVKVKNELLEETIIHWHGILQKKTPWMDGTPYVNQYPINPGMTYTYKFETDGAGTFLYHSHMNFQRAYGYSGPLIVECQDYTVYIHDLAVNLSWTKVRNVFINGKAGIQETDLTVSNKSLHTYSDFQFHTGECYILRLIPAFYTNFLAEFSIDSHNLTVFDSDGVCIEPVQVESILLNSGERFSVKIEATKESGWYWIRVRSNVQGDGIQGAIIKYNGEDGFLESFSIDYILEGPQFNVVWDDRLQGKDSGDIPIINARSCEEKIPCNPEKVFYMTFDERDGTLVAYDFSMDDIFSQHFKNLSLLQTQGMINEKEIFCNKETLRKDGINCTNPNRCECHHMIFAPCNTCIEMVFTSPNFEAHSIHLHGYKFFVVGQGYLSVEDLQKFQETDKVSPYPRQTESPLLKDTFLVPAYGYGIIRFFTDNPGYWILHCHMEAHLELGKYVIIKICEHEDMLDPPEIKLIGFLINFKVYEVGVFGKRKGKENAKLNLKINIVDANALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ01</accession>
    <name>T1GJ01_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras-related protein Rab-14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ01"/>
    <dbReference type="STRING" id="36166.T1GJ01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003438-RA">
        <property type="protein sequence ID" value="MESCA003438-PA"/>
        <property type="gene ID" value="MESCA003438"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_0_1"/>
    <dbReference type="OMA" id="NKMNYVE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003438-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="25823" checksum="7055F57C525D1463" modified="2013-10-16" version="1">RRPGRPKQGGLRLKDDVKLLNIPDWKRTAQNRRAWKNISTKRNLPEVMANCPHTIGVEFGTRIVEVADKKVKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDIARRSTFNHLSSWLTDTRNLTNPNTVIMMCGNKTDLESTREVTYEEAKQFADDNGLYFFETSAMNGQNVEEAFIEMARIIYQNIQEGRLDLNASESGVQRRTTIASGSLNGKDPDVKENCQCVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ39</accession>
    <name>T1GJ39_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ39"/>
    <dbReference type="STRING" id="36166.T1GJ39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003480-RA">
        <property type="protein sequence ID" value="MESCA003480-PA"/>
        <property type="gene ID" value="MESCA003480"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052004_0_0_1"/>
    <dbReference type="OMA" id="DYACNIP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08308">
        <property type="entry name" value="Death_Tube"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029397">
        <property type="entry name" value="Tube_Death"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14786">
        <property type="entry name" value="Death_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="51"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003480-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="305" mass="33740" checksum="26F94A578559D351" modified="2013-10-16" version="1">MYGRNTELRKLSESDIRKLGDILDLSNDWKLLIGHIPKDATTSLETYYQAKYNNEHIRLIENVSEQQKRSPTEILLDEWGTSGKKRPTLEVLLNVLVKAQLLRAADLVALNFLNEPPPERPKRGPGAPIPIDIPMDTEEIREIEADLNRGSYPGTDTLNDNANVSLLLNNNRDLYDKYRNSQHISSIENENIPILSALATSDDEDIENPSKEWPPQEVPDPNVPDLSALQHSTALPDLSSLLSNDDASDNSSSSSVNSENVESSISSNVTPNINDSSLTTITETSGDNSFDMSNGSFKSSENIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJT8</accession>
    <name>T1GJT8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJT8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003742-RA">
        <property type="protein sequence ID" value="MESCA003742-PA"/>
        <property type="gene ID" value="MESCA003742"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944359_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003742-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6917" checksum="A3A95EA4E53BCDB6" modified="2013-10-16" version="1">MGRSNGQRCQRTRNTLFLLAGFCRRIINIKVKQQDASDTESQYMSRKSHGQRDKNSNVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKN6</accession>
    <name>T1GKN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02042045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKN6"/>
    <dbReference type="STRING" id="36166.T1GKN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004063-RA">
        <property type="protein sequence ID" value="MESCA004063-PA"/>
        <property type="gene ID" value="MESCA004063"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1620936_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF105">
        <property type="entry name" value="CYTOCHROME P450 4P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004063-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19459" checksum="A316FB28CBCBABE5" modified="2013-10-16" version="1">MTRGLYLGILIFFQKQKYSPEKVRSKQQKPLIYQLMSVVGTLSLPCCLLQQNMRLFRYIRNLSNKYQESFLLHVVLGSAYQIIRAEDLEDVMKEPDLITKGIYYHFLEDFLGEGLLLSTDKKWHSRRKLLTPSFHFNILERTCYVSAIDYFLYHNQFEDVEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKV4</accession>
    <name>T1GKV4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ion transport N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKV4"/>
    <dbReference type="STRING" id="36166.T1GKV4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004135-RA">
        <property type="protein sequence ID" value="MESCA004135-PA"/>
        <property type="gene ID" value="MESCA004135"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870278_0_0_1"/>
    <dbReference type="OMA" id="YSNCNES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ion transport N-terminal" evidence="1">
        <location>
            <begin position="93"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004135-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15317" checksum="4032DDA4D9B26881" modified="2013-10-16" version="1">LLGANSTESVRCSYYSNCNESDLRLSGDNTCTDSLVTALDDEALLICDAGSEMARTKVHFDDVSLYGTPKEEPMPTIPVVSEKVSANFLKNQLQSWFQPTDNRLAMKLFGSRKALVKERIRQKTSGHWVIHPCSSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL21</accession>
    <name>T1GL21_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004207-RA">
        <property type="protein sequence ID" value="MESCA004207-PA"/>
        <property type="gene ID" value="MESCA004207"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253104_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004207-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12520" checksum="562CD50E20394F73" modified="2013-10-16" version="1">MKRCHSFTSLSCQPKTFFFRQPSSRREINESAVIYDFGYFTSLNLKFLVTILAFFLDEASPKAAENITDYQKFEKNIHTQIDYRNRHWLKPQTKEDNEMRQKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL41</accession>
    <name>T1GL41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF243 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02108837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004229-RA">
        <property type="protein sequence ID" value="MESCA004229-PA"/>
        <property type="gene ID" value="MESCA004229"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051251_1_0_1"/>
    <dbReference type="OMA" id="RHAQHEI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588376" description="DUF243 domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="87"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004229-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="26635" checksum="C2616D115EE53FE7" modified="2013-10-16" version="1">MNFFVCVLALLAVASAKPQYDYNPGPISAPQVPFAAASGQYQVSSPDAISAPQYSAPQASGSHFSGSQLSAPHFGGAREVIQSHEAPLVTKQFFLHSAPEERSVFQKTKHIVLGRPQKNYRVVFIKNPSNNNANVHLSAEFAPQEEKTVIYVLSQKDSALDIDNIATPAPTQPSKPEVFFIKYKTPEEARHAQHEIQRQYDVLGGQTQFSDEGTAPVSSVVGSFSTPVGQAVSNLVNEYLPPRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLS0</accession>
    <name>T1GLS0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-L-fucosidase</fullName>
            <ecNumber evidence="3">3.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 29 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02035042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLS0"/>
    <dbReference type="STRING" id="36166.T1GLS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004483-RA">
        <property type="protein sequence ID" value="MESCA004483-PA"/>
        <property type="gene ID" value="MESCA004483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1333283_0_0_1"/>
    <dbReference type="OMA" id="IRANHSH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016286">
        <property type="entry name" value="FUC_metazoa-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000933">
        <property type="entry name" value="Glyco_hydro_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057739">
        <property type="entry name" value="Glyco_hydro_29_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030:SF37">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01120">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00741">
        <property type="entry name" value="GLHYDRLASE29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00812">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 29 N-terminal" evidence="8">
        <location>
            <begin position="60"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="206" mass="24259" checksum="1B6B3CC5F4BCBA10" modified="2013-10-16" version="1">MPSSYKPLKWGEEGNFRESNLPQEEVVMLRPERKKFAFNLFIVLVFVNFLELALCSKCRAKSEDCSKEYNPTWESLDTRPLPQWYDNAKVGIFVHWGVYSVPSFGSEWFWINWKNGEKKYVDFIEENYPPGFTYQEFAHEFKGELFNATQWAKLFRDSGAQYVVLTSKHHDGFCLWPSTRSFSWNSVDIGPKRDIVELESGEQCYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM04</accession>
    <name>T1GM04_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013683">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM04"/>
    <dbReference type="STRING" id="36166.T1GM04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004572-RA">
        <property type="protein sequence ID" value="MESCA004572-PA"/>
        <property type="gene ID" value="MESCA004572"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1074759_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004572-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="259" mass="29665" checksum="04183C888D733F19" modified="2013-10-16" version="1">MLKLGDIGAHEANFMKMLIISTFLYSLTITQVYDSGSILSRSLGHQNRGLAPQIPIGINYRQYPFLLAIATATCVEFKEATSKEYIKDPSEFTAATDVPIARKKPRKKNLNPSMMTWASAITDTTTICRSTERVQYSTETLKLETIPTDLDIESNWKLCADGITVVSEYIGDDEYDRAVVEKVYQKSRGRLESRKFYQGVIKCRNGYTLTSFCKDRSGKLKNREFLVVRAICYLRRILQRSAMYDQSRMNPKKNRLFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM70</accession>
    <name>T1GM70_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM70"/>
    <dbReference type="STRING" id="36166.T1GM70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004638-RA">
        <property type="protein sequence ID" value="MESCA004638-PA"/>
        <property type="gene ID" value="MESCA004638"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087568_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22198">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22198:SF1">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004638-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="12976" checksum="9C9BD13F9B86F039" modified="2013-10-16" version="1">MINTAQTDPSNFYSGIRVASLSKFEPKNLKYTAAHTLDLYSEVAAICRPPLVIAPNEAGGLKKHQTPSTGYIVSVFKMKISYLILTIMARNGVFVKDYKSRKNPGIYHGAIQEIKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM75</accession>
    <name>T1GM75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="4 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="18">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4 18"/>
    <dbReference type="EMBL" id="CAQQ02176024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM75"/>
    <dbReference type="STRING" id="36166.T1GM75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004643-RA">
        <property type="protein sequence ID" value="MESCA004643-PA"/>
        <property type="gene ID" value="MESCA004643"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_0_2_1"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000029">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="17 18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 18" id="KW-0408">Iron</keyword>
    <keyword evidence="14 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="40"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004643-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="286" mass="32521" checksum="B108CA90D4B57617" modified="2013-10-16" version="1">MALVNGSRVILARNGIRALSTTSISNQEAQPKPARVKTFEVYRWNPEDGKKPHMQQYKINLNECGPMILDALIKIKNEMDQTLTFRRSCREGICGSCAMNIGGTNTLACISKIDTNTSKSTKIYPLPHMYVLRDLIPDMNNFYEQYRSIQPWLQRKNEATEQKGKAQYLQSVEDRTKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWIIDSRDESTQQRLDKMKDKFSVYRCHTIMNCTKTCPKGLNPGRAIAEVKKLLSGLATKPAPKMETAALHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM82</accession>
    <name>T1GM82_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02039543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM82"/>
    <dbReference type="STRING" id="36166.T1GM82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004650-RA">
        <property type="protein sequence ID" value="MESCA004650-PA"/>
        <property type="gene ID" value="MESCA004650"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151042_0_0_1"/>
    <dbReference type="OMA" id="RDMKNEG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000124">
        <property type="entry name" value="Cyclin-dependent kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF233">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="43"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004650-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12314" checksum="CD923304F61FEAB6" modified="2013-10-16" version="1">MSSRPREGRPDDQHGSQKHLTLAEKQKYIENYDFKYCDESSKYEKVAKIGQGTFGEVFKAREKKTNKKFVAMKKVLMDHEKEGFPITALREIRILQLLKHENVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN07</accession>
    <name>T1GN07_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120069">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120070">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004946-RA">
        <property type="protein sequence ID" value="MESCA004946-PA"/>
        <property type="gene ID" value="MESCA004946"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545205_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004946-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9584" checksum="06A406B8A94918B5" modified="2013-10-16" version="1">MDSPGAVGRRRGTSRVNAEDQALDQIAKEKSKKSICISRQTYLSFGREENIICGVFRRINHLNDIFFLYGKETRFLIIYLSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNI0</accession>
    <name>T1GNI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GNI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005131-RA">
        <property type="protein sequence ID" value="MESCA005131-PA"/>
        <property type="gene ID" value="MESCA005131"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_1"/>
    <dbReference type="OMA" id="WINLENA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="32"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005131-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="358" mass="40796" checksum="1E6DE8763EA68155" modified="2013-10-16" version="1">FRSYHQFRVEAEPINYPVRIVSYKDPIDELRNYYKILGVERCASQSEIKNAYYYLAKQYHPDNKKSEKHQKLFQEIVEAYNVLIDDNKRNEYDKYGEIKDIKGYLKQIGEREKTKHVNENIKLSDLMQNGCTRTGKSDFSVGLAPNEATINIDFIQSVQGLKKEFVIKCLRKCPRCSGAAELKSHTSKCHKCNGTGIYRLYAKTHTTNKVCDTCKGKRVLKKSCCKFCDNKGYLFQNQPVYIVIPPGTCSGDVINVKNPNGEIPNKNIAVKXISYIKVAVANSVTIKNKVPSWDLFCNVINVKNPNNTIHGLVDVKIPPGVESNSKIPLSGRSHIVTFKLEVPKTINENVRKALTEWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNT9</accession>
    <name>T1GNT9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02109671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02109672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GNT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005250-RA">
        <property type="protein sequence ID" value="MESCA005250-PA"/>
        <property type="gene ID" value="MESCA005250"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1125646_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005250-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="27698" checksum="D9164E1B4F9F26D9" modified="2013-10-16" version="1">MSSLDHLWKHNCSYNXXXXEEKKSLITRNLQETLGEDKLNVILKERDLEDLLGLTSLVTQHDAEKAGAEVVKQVEYPLLSGLFYPGLQALDEEYLKVDAQFGGVDQRKIFTFSEKYRPALGYEKRINFMNPIVPGLAGGKMSSSREDSKIDLLDSPANVKKLKKAFREPGNIADNGLWSFINHVLFSLCKEGEGFTVSRKPENGGEKTFTQIGYSRMVLCTSNEDHTEVEPIILPASVQPGDILLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNW9</accession>
    <name>T1GNW9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="9">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the BCKDHA family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="9"/>
    <dbReference type="EMBL" id="CAQQ02020837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNW9"/>
    <dbReference type="STRING" id="36166.T1GNW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005281-RA">
        <property type="protein sequence ID" value="MESCA005281-PA"/>
        <property type="gene ID" value="MESCA005281"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_2_1"/>
    <dbReference type="OMA" id="GMFRGVN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000015">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="10">
        <location>
            <begin position="95"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="10">
        <location>
            <begin position="302"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005281-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="414" mass="46745" checksum="358EF150B6B9AA44" modified="2013-10-16" version="1">MSFLRNSLKLFRTVPSATRTFRCFASQSSQSNQVPLDDPSRFPGAHAPFVSVPNLTLPENVAPVPIYRVMNRDGVIEDPSQDPNLSQEVVEKMLRSMISLNTMDKILYESQRQGRISFYMTNFGEEASHIGSAAALENQDLIYGQYREAGVLVWRGFSIQQFIDQCYGNSDDIGKGKQMPVHYGSKDLNFVTISSPLATQMPQAVGAAYAFKREPNNDRCVVCYFGEGAASEGDAHAAFNFAATLDCPVILFCRNNGFAISTPSHEQYVGDGIAGRGPAYGIATIRVDGTDVFAVYNAMKVSHHSTSDDSTAYRKAEELEVWSTVESPIYKLKNYMAKKGWWNEESETEYVKDIRKQVLKQINVSEKKQKDYYGEMFQDVYEEMPQHLKEQLKQLEEHVARNKQHYNLSEFKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPE3</accession>
    <name>T1GPE3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005471-RA">
        <property type="protein sequence ID" value="MESCA005471-PA"/>
        <property type="gene ID" value="MESCA005471"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3417471_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005471-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="26" mass="3304" checksum="1FEC49CAD30C19CE" modified="2013-10-16" version="1">MEKYGIWKKNSRNVDFKIKIHLFFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPE9</accession>
    <name>T1GPE9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005477-RA">
        <property type="protein sequence ID" value="MESCA005477-PA"/>
        <property type="gene ID" value="MESCA005477"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148724_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005477-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="13142" checksum="AF4346EDB38B8EC6" modified="2013-10-16" version="1">MLKKNSKSREKRWLLFPRTSPTRTQFIAGIGIPVEDLLYESITSGYVFKAEYYLPYLAEQWHTFTSMKIGELPIQGRRRRSSETMSLDMLTKTLPIHVENFMVLLPMKSNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPM2</accession>
    <name>T1GPM2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPM2"/>
    <dbReference type="STRING" id="36166.T1GPM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005556-RA">
        <property type="protein sequence ID" value="MESCA005556-PA"/>
        <property type="gene ID" value="MESCA005556"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329234_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000025">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005556-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23102" checksum="583DB6EC2858B53D" modified="2013-10-16" version="1">MHQPKRVSLMNHYIFRINHFSHRNIAFVRGIRRLGCEMFTNDGATILRSVGVDNPAAKILVDMSKVQDEEVGDGTTSVTVLAAELIREAEKLVDQKMHPQTIIAGWRMATAVARETLQNAAMDNSKDDAKFKEDLLNIARTTLSSKILYQHKDFFANLAVDAILRLKGSGALNAIQIIKKSGGTLEDSFLDEGFLLDKKPGMHQPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPQ5</accession>
    <name>T1GPQ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02057061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005595-RA">
        <property type="protein sequence ID" value="MESCA005595-PA"/>
        <property type="gene ID" value="MESCA005595"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707608_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005595-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8200" checksum="25BED6954BCAEB8B" modified="2013-10-16" version="1">MNALKWTSGISPTRGEILPDSNMMLLHFSGVFSSISSIYRPYDATPFLRRLFLDQQSPSAFIKSDCNVGNSVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPY8</accession>
    <name>T1GPY8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02141903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005684-RA">
        <property type="protein sequence ID" value="MESCA005684-PA"/>
        <property type="gene ID" value="MESCA005684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_842757_0_0_1"/>
    <dbReference type="OMA" id="IECANKA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007931">
        <property type="entry name" value="TsetseEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05267">
        <property type="entry name" value="DUF725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588426" description="Protein TsetseEP domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Protein TsetseEP" evidence="3">
        <location>
            <begin position="60"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="330" mass="35651" checksum="7B752209B684E800" modified="2013-10-16" version="1">MNIKVILLTIFALSVNGLPRAFPVSLTNFKPTHVLSYVQQLAAQQALMSRMAVSGRMSNAECYQKSSAALIECANKAEEGNFKCENILNVAQAKADDQLEQARKSLDIQSREIQSNLNSCKSSDSFTAVESLECYETQGQLNVSPVSTLSSKARSYRRAVDNIINAATTESSGCSFDVSTNSEAVSAKIYNSLNQCIANGEWIDVEIPDFGPVETPSETTLKPDTTTKSTETTHAEDTTRPQETTTTQTTETTKGIDTTKSTETTKATETTKATQTTLDTTKASVTTLQPTTAQNPETTAPATVPENPEPNTTVKDVPDATNEPKPENEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQN8</accession>
    <name>T1GQN8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005949-RA">
        <property type="protein sequence ID" value="MESCA005949-PA"/>
        <property type="gene ID" value="MESCA005949"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1557047_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005949-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="19743" checksum="F56FC3215E5E370B" modified="2013-10-16" version="1">MRYCILHKFEWKWGGFEYDEYVLCIVNGYGFEIVKSKNSLTSMVKEVNTALSNCQEILGIPKYKLISSNPKIPTIYIFYITHTIGTRRIIANVDSPTSKIAKFLVQKFNCLRPPEGLFVKNSFEVADKLSKLHLDLNEEMVLFDVTAIYPNVLIDEALDCFFKAALQKKVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQP4</accession>
    <name>T1GQP4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Congested-like trachea protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02008937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQP4"/>
    <dbReference type="STRING" id="36166.T1GQP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005955-RA">
        <property type="protein sequence ID" value="MESCA005955-PA"/>
        <property type="gene ID" value="MESCA005955"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_6_1"/>
    <dbReference type="OMA" id="CEAGICD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF4">
        <property type="entry name" value="CONGESTED-LIKE TRACHEA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="42"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005955-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14429" checksum="D46381267F3174F7" modified="2013-10-16" version="1">MRSVYKGFCATLMRDVPGTGAYFLAYEYVQDKVKEKSGSQTLSTLSTIFAGGIAGIAYWVIGMPADVLKSRLQAAPEGTYKNGIRSVFAELMKNEGPLALYKGVTPVMIRAFPANAACFIGVEVSMKLLNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR73</accession>
    <name>T1GR73_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">von Hippel-Lindau disease tumour suppressor beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VHL family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02080644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR73"/>
    <dbReference type="STRING" id="36166.T1GR73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006149-RA">
        <property type="protein sequence ID" value="MESCA006149-PA"/>
        <property type="gene ID" value="MESCA006149"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323040_0_0_1"/>
    <dbReference type="OMA" id="INISGRH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05468">
        <property type="entry name" value="pVHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.780:FF:000001">
        <property type="entry name" value="von Hippel-Lindau disease tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.780">
        <property type="entry name" value="von Hippel-Lindau disease tumour suppressor, beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024053">
        <property type="entry name" value="VHL_beta_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037140">
        <property type="entry name" value="VHL_beta_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036208">
        <property type="entry name" value="VHL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022772">
        <property type="entry name" value="VHL_tumour_suppress_b/a_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01847">
        <property type="entry name" value="VHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49468">
        <property type="entry name" value="VHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="von Hippel-Lindau disease tumour suppressor beta" evidence="2">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006149-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11555" checksum="C218CE3297C8A095" modified="2013-10-16" version="1">MQPRLISGESVQEVFLLFINKSGRSADIFWINYNGEQKYFGTIEPGAQKKINTFSNHPWVFRSKITGEKLHIENKDILWQLQAFIMAEQLDVSFVFTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRI5</accession>
    <name>T1GRI5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat-containing protein on Y chromosome</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRI5"/>
    <dbReference type="STRING" id="36166.T1GRI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006270-RA">
        <property type="protein sequence ID" value="MESCA006270-PA"/>
        <property type="gene ID" value="MESCA006270"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1715343_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF6">
        <property type="entry name" value="EF-HAND CALCIUM BINDING DOMAIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006270-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17372" checksum="C18E44D89185C034" modified="2013-10-16" version="1">MLQSYGDLTRHFAYESRLAYYYNRDSRELVVAGRIVVAIKCCAKLRLDLTDGNTHSAPVSVVLYNKLFKNIVTCGLDSYIIVWDPFTGKRQLLMKNCHTRVIYGENVDIEITAACFNTMAQFLLTGARDGSVKIWNYNNATCIRNMNIESGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRI7</accession>
    <name>T1GRI7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRI7"/>
    <dbReference type="STRING" id="36166.T1GRI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006272-RA">
        <property type="protein sequence ID" value="MESCA006272-PA"/>
        <property type="gene ID" value="MESCA006272"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_9_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006272-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6793" checksum="CC5EA0EFBEB891BC" modified="2013-10-16" version="1">MPGEVIDIRVNVGAKVEKGQPFVLSAMKMEMVVQSPKAGVVKKLEIQKGMKLEGEDLILIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS83</accession>
    <name>T1GS83_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS83"/>
    <dbReference type="STRING" id="36166.T1GS83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006533-RA">
        <property type="protein sequence ID" value="MESCA006533-PA"/>
        <property type="gene ID" value="MESCA006533"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_751377_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055113">
        <property type="entry name" value="Med14_RM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056879">
        <property type="entry name" value="RM3_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22981">
        <property type="entry name" value="RM2_Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25065">
        <property type="entry name" value="RM3_Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM3" evidence="4">
        <location>
            <begin position="81"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00032007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006533-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="369" mass="42556" checksum="78C27163F82C7321" modified="2013-10-16" version="1">DRLDENIKIDEYIPGVKLTVSYWRELTTKDPKSELGYKLTIQTDPNDPTRTLSVIHIPSIGQKESNEIADRAIHSDHLSMERLIVHTVYIRSVQRLTDLKAEFNKFLKNIEYFIQGTPAILTIPILNPCLRAEQIHVTVDTHTGMLKCHVPKHLDCPIMPEFQLALNGDTSKLAQLSTELRYWITHKRCEITLQHLPAVIMENLPFLSPPTLPLLKSGRQKIFVKLKRHPQIVLILHLKEKENNPNEIDYTFHLGFLNFVSQEDPAEGQQQVPHPPEIPRFYTKLLKLVEFDTFVATHGPGTHVNDVPSYKRKLGDFIGQPASKQAKTIIRRTSSQNWLMSLQCVMRRFRSLLCLENSPREIYHTVAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSZ9</accession>
    <name>T1GSZ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSZ9"/>
    <dbReference type="STRING" id="36166.T1GSZ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006815-RA">
        <property type="protein sequence ID" value="MESCA006815-PA"/>
        <property type="gene ID" value="MESCA006815"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015769_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11787">
        <property type="entry name" value="SH3_SH3RF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11783">
        <property type="entry name" value="SH3_SH3RF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF51">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="110"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="170"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="319"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="242"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="559"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="564"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006815-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="604" mass="66830" checksum="3875C5CF84820304" modified="2013-10-16" version="1">VIVSSHSELRCPECRVVVECKIDELPPNVLLMRILEGMKNAVKPPSQPPDSSVTQASSTMQNHQISTNCPQTPPVHIQNHQANKLHQQQILLQQKARLQAFQMQPQPNRRVVPHAFAIFDFASKESSDLNFKKGELIVLKRKVDSNWYFGECNGREGMIPSNFIQVMVPLPVPQCTALYDFKMGPQEEEGCLTFEKGAMIKVLRRVDQNWVEGRIGASIGIFPIAFVEMNPLAKKLMETHIDTSKNHPNEPRKLPSLPTEEPSQSSSSSNSSSNPTSPQNRAFSGVKYRDYKDKRLSLNANISTRVPNVPLCNILINVPLPWGYLALYPYKPKKPDELELKKGCVYIVTERCLDGWFKGKNWRDKTGVFPGNYVTPLRSRDQQQLIHQGNITQTNIITSSGDNSATSTAVPELPPRSAGTTGTASLLDSIFHRKPSEPQQQQNNAATNSTAVNLMKRLNIMKRSKSPVQGNPNPAFEDTTVVATPAVVTSSPATKQVAHVRSGSCPSQLLQNVPVDVGGASTTTGCGTLFGFGSQRHKPQKEKVSVLTVRQQMDNHANHRKSLSMDQNVAQSKPQNTSPSAGMRHSNVTGQPVVQQMKKSELFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTG7</accession>
    <name>T1GTG7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006998-RA">
        <property type="protein sequence ID" value="MESCA006998-PA"/>
        <property type="gene ID" value="MESCA006998"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203565_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006998-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12675" checksum="F6C54EF5A5ABEB27" modified="2013-10-16" version="1">PEKGTEFRINLPEGFNFTSLKDTDKLPNVIDPATYHVKIFEFLGDEAQFVLITGERCKYRYNSTSENYERQFCWNKPSFCRGNIIRCFQNDGPESLCYDESQDKVTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU03</accession>
    <name>T1GU03_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007202-RA">
        <property type="protein sequence ID" value="MESCA007202-PA"/>
        <property type="gene ID" value="MESCA007202"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_772309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007202-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="359" mass="40556" checksum="151B07E7BFE01E06" modified="2013-10-16" version="1">MIHSTSNITSCTVLNEGKTNFLTDNEEELLKQIRNYGKFNFDSMNIAFNDVFKNEDYNDPKVDHETMYKCLSDGGAIADEMNANNEQIIVDFSANRRLLRDSIINITVKETKELIECQNKDKTDVSPLNLSNFISPTDSKTTTKTLDTTSTKSSQTDVSVKKKRSFRPKITINNCNGIINLRNIANLTINCAKESSIRKEKSSAESNSLNKECSVKEEETVSSTTKDESDEIEGNCEFYNRLLNEIKKSLIKKTQTYPLPSSGEEENIDVPVEEDVLATPDSTDEIENTANHKFVLKNFENLKVIFENSNNCSKIVRIKFQISPVGVSEIGISVNPQKRSRIFFLVAGTFSYTPYNPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUH4</accession>
    <name>T1GUH4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ataxin-2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021489">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021490">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUH4"/>
    <dbReference type="STRING" id="36166.T1GUH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007387-RA">
        <property type="protein sequence ID" value="MESCA007387-PA"/>
        <property type="gene ID" value="MESCA007387"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248072_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007387-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="24428" checksum="1EE7963B695D611C" modified="2013-10-16" version="1">KQQIQGQNIKTHAEQPQTQQSNTPSQPTTQQIAQQDQQQSYQMQTPPTNVDSQSNQIHSAGSNLKPGVNTVTDSPSEKSTPPTVAKKHVLNPAAKPFTPRSPSTPTQSRPHTPQTPPYQQMVRYYSEPPPQPVQFLAQTPPSTTPSPGQHQQFHQPQPQPSPQTGAPQQFVPGGQQPPPQPTHYQVVPVIPQQLVSHYYQGAAQHPQQAQQFQIVMPGHATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUL1</accession>
    <name>T1GUL1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007426-RA">
        <property type="protein sequence ID" value="MESCA007426-PA"/>
        <property type="gene ID" value="MESCA007426"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007426-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16927" checksum="0A534140ED243818" modified="2013-10-16" version="1">NKELEEKQHTLQQELHEKDEKVKLLQKTVDDLKTTRNIIESKMKTWGGGNEKIGRLSKLVKVLEDESKTKDSRLENIFREASNYQNISKKLEAECQELKAHLESLKNTNMKLDQALNDKQKEFDAIAKKLEHLEHVEKEIKIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV44</accession>
    <name>T1GV44_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV44"/>
    <dbReference type="STRING" id="36166.T1GV44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007626-RA">
        <property type="protein sequence ID" value="MESCA007626-PA"/>
        <property type="gene ID" value="MESCA007626"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278942_0_0_1"/>
    <dbReference type="OMA" id="NNCELNS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007626-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="24006" checksum="646D53FEAD4168A3" modified="2013-10-16" version="1">MPFIDDALLWCPDNDGRMVGGLVDISTCLQDTSQNVNVSQNQSTDVNSSNNCELNSLDNLGDSSEELFRQFDGSFEIESLLSDLTEIKQEDNNNNLSLLPEHQQQQQHHQHHQLLSSNQQQNQFQQQTQYNQTETALLALAASVTAANSNQQHQQQISSLHRNYVSSTTGDNPNQNNSVNNNGSSSSSNNSNGSRYSIAANPLLAEKLLYQIVLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV56</accession>
    <name>T1GV56_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007639-RA">
        <property type="protein sequence ID" value="MESCA007639-PA"/>
        <property type="gene ID" value="MESCA007639"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3417473_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007639-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="26" mass="3325" checksum="61D10C165F04A7D1" modified="2013-10-16" version="1">MYADTKWHHMDIWQFTILCTNNIKKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV94</accession>
    <name>T1GV94_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02060829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV94"/>
    <dbReference type="STRING" id="36166.T1GV94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007684-RA">
        <property type="protein sequence ID" value="MESCA007684-PA"/>
        <property type="gene ID" value="MESCA007684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_802375_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="141"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="346" mass="40345" checksum="3940841D10B1D46C" modified="2013-10-16" version="1">MGDWGAHIWSEECKDFPIIRMDNDKKQCTSGCWSTQRCSLFFTGGKDGKLEFWDLLLDFKKPIYIVDTQYPILCVAISEDGKYVSCGLVNGDFKVYEVDEAFQHCTAKEKMLTTAMFERELQRLKNLDGRLREIILREKALQKAMKENEKVKKKNLDGEEEEELEEEEEELVEAMGEEFEGEEDEYIDPDPVLTAATKNFYDLSEQIRIKRNSSIVLIAYLKARESFLSDFFPSKLYTENDYYLPSHLQPLHICKLADDEIDDKSTILREERTYDKKGESFDNKKYELVTVLELKLAFQLEVDFLPRNLKNKSWAFIAKYEFITVPGMRIGEHSCMIPSPSGKLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVS4</accession>
    <name>T1GVS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007890-RA">
        <property type="protein sequence ID" value="MESCA007890-PA"/>
        <property type="gene ID" value="MESCA007890"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592515_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007890-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9206" checksum="85F9EAC2F9246326" modified="2013-10-16" version="1">MHIPINGFVSGQRIPITCLRPDSMSKLKLLVLIRILQDMFPLLWARSHWLPSSSQLHNNISHQLLYNQLAQLAHPMKVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVY5</accession>
    <name>T1GVY5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vps16 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GVY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007953-RA">
        <property type="protein sequence ID" value="MESCA007953-PA"/>
        <property type="gene ID" value="MESCA007953"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029487_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040057">
        <property type="entry name" value="Spe-39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13364">
        <property type="entry name" value="DEFECTIVE SPERMATOGENESIS PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13364:SF6">
        <property type="entry name" value="SPERMATOGENESIS-DEFECTIVE PROTEIN 39 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps16 C-terminal" evidence="6">
        <location>
            <begin position="121"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007953-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="447" mass="51288" checksum="C77C2D0CC831EA0C" modified="2013-10-16" version="1">MDSAEAYWNCSTSNAFSFDDDDTDGKRNSKYLFDDDLPAPKVEILESTNSNIHDIISDEDLKTILDEQSLESYTVPKGISSEDELKLLRRRLQYVLHSPSIRFTLHKLLLGKRCLLEAYKSLEDKEKLLESLIETRNGDGILAVLIFLSKTLNEKQFNRILLTYRDACDIYVNYLIEMKYTKAVDLLVMLGRSQEAIMLQIKTELGRNNKLKLRQLLNEYVNSPGVIPIYCQILNNAISLAELLESSGKDDMSPVEVLYTACSRHHWKDQNLSDPWSPYKLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPIQFEWVGLNERARSKAFMDLESIFEKTSFLVKQKQFHISFSLDIAILRLLLLEAPQAVLYLFLSKVNNNSKKLTLAKQVKCNKAIVDSLVALKEISELEKFKLSLTEGTEDFFYCEQSIKNLGSKKWSNPNLKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWI4</accession>
    <name>T1GWI4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008161-RA">
        <property type="protein sequence ID" value="MESCA008161-PA"/>
        <property type="gene ID" value="MESCA008161"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1262827_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008161-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="25336" checksum="E08CC9F4A4515CD7" modified="2013-10-16" version="1">MADYCFKESQLNGFSEGCLFEKFLDSVRSVFSSNFDIPEALDYFTLVVERYMDTMDYDSRLFERYTEVVASMSQNSINSLTSPANWVDISIPKLKKATLVRCYLVLYSSQISNPLIWLNPLIDAFAVKTEDCLFFERHFAITLHAFNDDKNACAWLMELMIQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTRFSFYSIFSFSPLKYSLDVEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU6</accession>
    <name>T1GWU6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWU6"/>
    <dbReference type="STRING" id="36166.T1GWU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008277-RA">
        <property type="protein sequence ID" value="MESCA008277-PA"/>
        <property type="gene ID" value="MESCA008277"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023330_0_1_1"/>
    <dbReference type="OMA" id="KVNTMYQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19954">
        <property type="entry name" value="serpin42Dd-like_insects"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="8"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008277-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="362" mass="40794" checksum="C130D0756247D984" modified="2013-10-16" version="1">MEEPLFSVELYKCIDIKNNLVFSPLSINACLGLAYMGAGGDTAEEFSKRLRFGSLSKEEVATNFFKIFGESKAMRSVQIATKIFINKEFNISDRFSALSKRSFNSSVENMDFSNSSDCEKSINSWVEKETNSKIKNLIAPGTVKAETSALLVNAIYFKGEWEKPFSQSNSTKMPFFSTENKSVEVDFMIHNEPDVHFGDLKDLESSAVELKYKGTDLSMVIVLPDKNEPLTKLAGNIQNNYDLMGISGKLSSRQLDIYIPKLEIETEIDLESPLFKLGIKTAFTDCADFKEMFQGTIKPMKITKAVHKAFIKVDEGGTEAGASTEITITMRSLPRVFKADHPFFWFIKDTNYIYFMGQVTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXQ2</accession>
    <name>T1GXQ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008606-RA">
        <property type="protein sequence ID" value="MESCA008606-PA"/>
        <property type="gene ID" value="MESCA008606"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834042_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008606-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7511" checksum="815B5BC8B52E3E3B" modified="2013-10-16" version="1">MDARNDAKKTYHLFLQTLPFKIGYLVVEDAFRLTIKDFSGYVSDDDPIKSLIDKLSSDYGVLGFDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXZ0</accession>
    <name>T1GXZ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389015">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008704-RA">
        <property type="protein sequence ID" value="MESCA008704-PA"/>
        <property type="gene ID" value="MESCA008704"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280588_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008704-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11564" checksum="DA8863E61B07C105" modified="2013-10-16" version="1">MDLSIGTDTYKKQYIIVVKTTATDILGIYKPQNFSGWIDEEYDKAVAKKKRSALSENRPKKDEGYERSVSKQKKGGCEIGQPSLECWSLWEIGIMPKNSTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY10</accession>
    <name>T1GY10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008725-RA">
        <property type="protein sequence ID" value="MESCA008725-PA"/>
        <property type="gene ID" value="MESCA008725"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815381_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008725-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7412" checksum="8D1044C27E0BA1AC" modified="2013-10-16" version="1">MSTKFVSISPNLGIRVPSESTSSATSSDEILSVGCRGKKRRLDHLTWEEKKIEESSCCSNIKRPQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYJ3</accession>
    <name>T1GYJ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYJ3"/>
    <dbReference type="STRING" id="36166.T1GYJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008922-RA">
        <property type="protein sequence ID" value="MESCA008922-PA"/>
        <property type="gene ID" value="MESCA008922"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063523_1_0_1"/>
    <dbReference type="OMA" id="NMPYGEQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19744">
        <property type="entry name" value="bHLH_TS_dAS-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF153">
        <property type="entry name" value="ACHAETE-SCUTE FAMILY BHLH TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="64"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008922-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="233" mass="26120" checksum="9B9C66D11F99CC03" modified="2013-10-16" version="1">MALGQTYSHNHQSAMKPLAIAPAPVSRPTTVLGMNGINLPQQQFQVGGKQQKYNYRTAPYDQPASVVKRNARERNRVRQVNCGFVNLRQHIPQSIVTELTNGGRGASKKLSKVDTLRLAVEYIRRLQDLIEESDSEASNSSICSQSSTAGSPPPPVYYPSSPESMQSVNQYHQYSETSISPTPSFHSDIDNCMMQTTSSVQQQQQQVFKFENFQADSPNDDELLDYISMWQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYM4</accession>
    <name>T1GYM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008958-RA">
        <property type="protein sequence ID" value="MESCA008958-PA"/>
        <property type="gene ID" value="MESCA008958"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954210_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008958-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14839" checksum="9524B02A7885C4E7" modified="2013-10-16" version="1">MTVIKQYLVLHPKQTSYDSHQRIIGTSAPKDRSLTKSCTYIPKNLPVTVIKDYICQIVDLEERSSRQVSESEKTIFSPCIVTSQANVLEMLENFSKCLRKGLRKYAPASLIFFVTERDIHKENGQYPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYR3</accession>
    <name>T1GYR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-glutamyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYR3"/>
    <dbReference type="STRING" id="36166.T1GYR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009000-RA">
        <property type="protein sequence ID" value="MESCA009000-PA"/>
        <property type="gene ID" value="MESCA009000"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1690693_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000001">
        <property type="entry name" value="Gamma-glutamyltransferase 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF72">
        <property type="entry name" value="GAMMA-GLUTAMYL TRANSPEPTIDASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009000-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17567" checksum="58777757420CCB4D" modified="2013-10-16" version="1">GWEEPTVAKLKGDYTVYSTPVPGSGSVLTLILNIMNNLLTSDEELLWHRVIESFKHAYGYRTQLGDLDFTPEAKEVFETMIDPEFAKSIAELKVFDNTTFTDFEYYGGNFSSPKDSGTANMAVLHPSGDAVTVTSTVNSYFGCKVRSPSTGIILNDEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZF8</accession>
    <name>T1GZF8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phospholipase D-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02070993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZF8"/>
    <dbReference type="STRING" id="36166.T1GZF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009258-RA">
        <property type="protein sequence ID" value="MESCA009258-PA"/>
        <property type="gene ID" value="MESCA009258"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943427_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009258-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14691" checksum="3CF03FE9CB55F4A4" modified="2013-10-16" version="1">MIDIAWLLGHYYFAGCLDSNLLVLYGDESEDLKTISKFKPQVTAIKIPMPPFSTHHSKVMLLGYKDGSMRVVVSTANLYDDDWHNRTQGIWISPTLKPLPEEANTTDGESPTGFRHDLMAYLAQYKLGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZJ8</accession>
    <name>T1GZJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZJ8"/>
    <dbReference type="STRING" id="36166.T1GZJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009301-RA">
        <property type="protein sequence ID" value="MESCA009301-PA"/>
        <property type="gene ID" value="MESCA009301"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1462917_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF69">
        <property type="entry name" value="SI:CH73-59F11.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="1">
        <location>
            <begin position="13"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009301-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="21874" checksum="77425EAC0D0B4505" modified="2013-10-16" version="1">MSWRNIDNDEKYKLIEMVKERSILWDSRLDEFKGADTLKMNAWDEVAKEFNICSNKAQRSFRILREHYRKHLANNNEDSWPYFEMMDFLRPIIKLRKKYSYSSSFETKTGKNVESFDDEEIPSSTMIEIPVHAELSDNEHTSRNGAGDMSPKCNIFPENPPKLKFLSLISRTFEISLKTKFENIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZQ6</accession>
    <name>T1GZQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009360-RA">
        <property type="protein sequence ID" value="MESCA009360-PA"/>
        <property type="gene ID" value="MESCA009360"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349044_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009360-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="10629" checksum="6FB4939EEB827F0D" modified="2013-10-16" version="1">MTPNLAVQRKVKTASKSVEGGQSGFHRGGGGLRDYNDTFSGRSFSRVLPSFQGKEVQRKPNYLEWEEIEAGNGAFKLRNVLAKEKVIPGYIGKPDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZU0</accession>
    <name>T1GZU0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009397-RA">
        <property type="protein sequence ID" value="MESCA009397-PA"/>
        <property type="gene ID" value="MESCA009397"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136297_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009397-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="13686" checksum="C5F1EC32F8F2B7FA" modified="2013-10-16" version="1">MKSCSIFIIPRKIDRRLMTQTEINIWVEMVPETFRILFITSRVEMFRNYHFHWNFFKHIVLNFYQHIDCTITLEMCWLREWQGNNFWTDGRFLIPCNGLILGILAGYPGLPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZW4</accession>
    <name>T1GZW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZW4"/>
    <dbReference type="STRING" id="36166.T1GZW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009422-RA">
        <property type="protein sequence ID" value="MESCA009422-PA"/>
        <property type="gene ID" value="MESCA009422"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1497926_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009422-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="180" mass="20021" checksum="CF8B9867F48955F9" modified="2013-10-16" version="1">MRARNTTAVFLCLSPSPCESIGSVSDDVFFEKENGNAEADEIGKPTSTTRPKSQIVENSDIQEYLHSFERRNSDTSFLVNRRSSVGERMNLAEEIRKLSDHLMMLAEINKTLGKDKEKEKDNSGELTPTPENSVSQPSFLPKPKTSKLSVRLQKSIEETPTLTTTSSTSSTSSVQKMVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H085</accession>
    <name>T1H085_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H085"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009558-RA">
        <property type="protein sequence ID" value="MESCA009558-PA"/>
        <property type="gene ID" value="MESCA009558"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500495_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009558-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9919" checksum="4042D5EEB06E01C3" modified="2013-10-16" version="1">MRKCLLCRKTSIEVPLHAFPKNPAIREKWLQFCNVPEVTKSAVLCELHFKDGYRSAVSVPDCNLYNLHLLEGVTLKRKEKINDSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0C2</accession>
    <name>T1H0C2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02054515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H0C2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009597-RA">
        <property type="protein sequence ID" value="MESCA009597-PA"/>
        <property type="gene ID" value="MESCA009597"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001283">
        <property type="entry name" value="Eukaryotic translation elongation factor 1 alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="430" mass="47238" checksum="D8C719D45CC69EF0" modified="2013-10-16" version="1">MGKEKVHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYNGQTREHALLAFTLGVKQLIVGVNKMDSSEPPYSEARYEEIKKEVSXXXXXXXXXXYSEARYEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMSWWKGWAIERKEGKADGKTLIDALDAILPPSRPTEKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMSVEMHHEALTEAVPGDNVGFNVKNVSVKELRRGYVAGDTKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRSGKTTEEAPKAIKSGDAAIVNLVPTKPLCVESFSDFPPLGRFAVRDMRQTVAVGVIKSVNFKDASGGKVTKAAEKAGAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1A7</accession>
    <name>T1H1A7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1A7"/>
    <dbReference type="STRING" id="36166.T1H1A7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009964-RA">
        <property type="protein sequence ID" value="MESCA009964-PA"/>
        <property type="gene ID" value="MESCA009964"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_203431_0_0_1"/>
    <dbReference type="OMA" id="QNCYSSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032034">
        <property type="term" value="F:myosin II head/neck binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051893">
        <property type="term" value="P:regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15551:SF3">
        <property type="entry name" value="LIM AND CALPONIN HOMOLOGY DOMAINS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15551">
        <property type="entry name" value="LIM DOMAIN ONLY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009964-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8236" checksum="C1CC3D43B0E35F46" modified="2013-10-16" version="1">MDHKIRLLFDGRGAAMIIESLRLFYHINCFKCCVCHIQLGDGLNGTDVRVRNQKLHCQNCYSSEDGIKFSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1M5</accession>
    <name>T1H1M5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02097808">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1M5"/>
    <dbReference type="STRING" id="36166.T1H1M5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010090-RA">
        <property type="protein sequence ID" value="MESCA010090-PA"/>
        <property type="gene ID" value="MESCA010090"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742951_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010090-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8117" checksum="0075133C60019720" modified="2013-10-16" version="1">MLDNPPGVPLCELSTSLFFVIPMILIFVLYVRMGIQIKQRTTEKLGVQQGSMQKESRHQQSRKAVIRMLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H226</accession>
    <name>T1H226_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02076198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02076199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H226"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010257-RA">
        <property type="protein sequence ID" value="MESCA010257-PA"/>
        <property type="gene ID" value="MESCA010257"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349057_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010257-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11223" checksum="3A25B89F4F1D5583" modified="2013-10-16" version="1">MNKGSGRISDCNKQNIKEIPVLLSRQLSPKSRSEFLAVFLWVHLFIDFRQAYVTPPRDELFRYADDIDVIGSTKLDVESIFLEIEKVKSTFDLMAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H281</accession>
    <name>T1H281_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02087897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02087898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H281"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010317-RA">
        <property type="protein sequence ID" value="MESCA010317-PA"/>
        <property type="gene ID" value="MESCA010317"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760705_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8058" checksum="CF50740F108DF19D" modified="2013-10-16" version="1">MLNATFPLEIIGQCKFNTTALSYGRIVLKWGEHLRNLNLADLFSKSLDVKRAQEVSKKLRDVACHRRTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2L1</accession>
    <name>T1H2L1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2L1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010460-RA">
        <property type="protein sequence ID" value="MESCA010460-PA"/>
        <property type="gene ID" value="MESCA010460"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126756_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005712144" evidence="1">
        <location>
            <begin position="17"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010460-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14285" checksum="9020FEB41055D0FE" modified="2013-10-16" version="1">MYFKVILIALLSFSNAERLENCKVGHKFGNTTMAKFKLSDFENNKPRSTEVLRTKVYFKYSLPVFSIFNSPTQFTFGAPRLNTTFGIYKSHRDYKKEMNPCSSVENITYFSGTQYVGAVFILES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2S0</accession>
    <name>T1H2S0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2S0"/>
    <dbReference type="STRING" id="36166.T1H2S0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010524-RA">
        <property type="protein sequence ID" value="MESCA010524-PA"/>
        <property type="gene ID" value="MESCA010524"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1167016_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="209"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="83"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010524-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="27425" checksum="03F3C962CFF86D2E" modified="2013-10-16" version="1">MLKDVTYHQLKAILDYIYNGEVNIPQDQVDGFFRAAEFLQIKGLLRNSLEDTIIDPTEPSFKEEKSSQDSIFALDRLSKNVGGDLPLTSRPVPPLVPRRFDPLVPSTSSEASTSSRKRTRRCRSENKDTHFEDPAIAYPSIVLYEHNEWEHESKNFLEEDINNMGFEIPTNPGTEFSKTNDLRPYGCDCGSLIKTKKHQTHECGVEPAFCCDYCSKKFKRQWDLKMHVSRRHVPKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2S5</accession>
    <name>T1H2S5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">nitric-oxide synthase (NADPH)</fullName>
            <ecNumber evidence="5">1.14.13.39</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NOS family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.39" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02188883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2S5"/>
    <dbReference type="STRING" id="36166.T1H2S5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010529-RA">
        <property type="protein sequence ID" value="MESCA010529-PA"/>
        <property type="gene ID" value="MESCA010529"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1490663_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004517">
        <property type="term" value="F:nitric-oxide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050607">
        <property type="entry name" value="NOS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410:SF1">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfite reductase [NADPH] flavoprotein alpha-component-like FAD-binding" evidence="15">
        <location>
            <begin position="89"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010529-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20678" checksum="18A82254B43D8ABB" modified="2013-10-16" version="1">MCAQEQCFRKWAPEVFKVACDSFCLEPDVSVMEMKSESLNINTVRFLPLKNSEPLEKLLAKYHNRKVFKFHAKHTPKSLTNLGENIKATICLEISSPGVKYEPGDHVGIFPANHWKLVDGIISRLTGCDFPEDIVQLQILKEKQSLEEANDEVKDIFYEPLDISYSTIHAFDMKIVIGDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H330</accession>
    <name>T1H330_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Antistasin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H330"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010641-RA">
        <property type="protein sequence ID" value="MESCA010641-PA"/>
        <property type="gene ID" value="MESCA010641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898631_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577338" description="Antistasin-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14935" checksum="11982896D329ED8F" modified="2013-10-16" version="1">MFTKICIVLLLANCFGNYVQTNKTTLCGTYIEECKTNSSPGWPLIKICYEDTNCSTCRCPILPYCPNDSLEKCCKSRSPECKCTCPRYCASEEENKYCCIADTTIPPNFNCECICLLGIQPFFSPNTLKSAAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H352</accession>
    <name>T1H352_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H352"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010667-RA">
        <property type="protein sequence ID" value="MESCA010667-PA"/>
        <property type="gene ID" value="MESCA010667"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032409_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010667-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13560" checksum="827F3357FC185CA9" modified="2013-10-16" version="1">NVSGVFILSKVEEGTSIGCIGWVQISVFIITERNLTPRCQVVKASRLSGLGSVVSGKVEVTNLVVSEADSMLLNLKWIVCLHAVQTLTPTKTWSERAYVVESLRLQVLIRILNNLLMSWIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3J6</accession>
    <name>T1H3J6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3J6"/>
    <dbReference type="STRING" id="36166.T1H3J6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010824-RA">
        <property type="protein sequence ID" value="MESCA010824-PA"/>
        <property type="gene ID" value="MESCA010824"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1042994_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010824-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="263" mass="28995" checksum="78EC9EBAED80A8AF" modified="2013-10-16" version="1">MFVIFPADELILMTYGSAIPVVKPRPTLDCLREPLVPIGDRSYSPSEILARSTPTPSSSSIQALMSSSSNYGIPDATKQRLQQLKASNVKLTPKEEEMLGAVSYSSDENLLLSDYGDRQGRGRSPSVFLGAPSSAYSSRPTSPSSSKSGKSGQQSQTQSKSKFRPPKLHISLGRTKPDLTESKRTKEDHILQVDNPAFTSENIRERNFDAFFEAGQSVYKLHPKESSEILDDRGAMGYSSEADAEVPQKGDRCHKNIYYECTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4E1</accession>
    <name>T1H4E1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="36166.T1H4E1"/>
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        <property type="protein sequence ID" value="MESCA011150-PA"/>
        <property type="gene ID" value="MESCA011150"/>
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    <dbReference type="HOGENOM" id="CLU_824617_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
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        <property type="entry name" value="VWF_A"/>
    </dbReference>
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        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
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        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37860:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37860">
        <property type="entry name" value="AGAP008810-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <feature type="domain" description="VWFD" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="151"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011150-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37822" checksum="902625AAB6BB9FF8" modified="2013-10-16" version="1">MNNEPFDDMIASNKVIQTKEEEFVNSWALDQCKGASKKVLSRPTPEIVNLCNQFFKSPHFTPCTHVINPTPFIDMCLDMGMNSIATILKENHPAQKGACTAALGYIEACASAKIPLRIPDQCVHCDLPNGTYVPEGTFLTLRDQNILDSTDIVFLIEAKPCNKDLFSTKSFQTLLESINDELSSIKMEKNRFSVMTFGGRVPFDKPRSIVYKNEVFTYDIRDLKQQIEHIEFMNGSNNDAFQALMAASKLIFRPGASKTIIMLPCTTCLSREMMYDYTSMLQLLNESGINLHILTDVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSILCNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4P5</accession>
    <name>T1H4P5_MEGSC</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4P5"/>
    <dbReference type="STRING" id="36166.T1H4P5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011261-RA">
        <property type="protein sequence ID" value="MESCA011261-PA"/>
        <property type="gene ID" value="MESCA011261"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444394_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039190">
        <property type="entry name" value="TTC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184:SF9">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="21659" checksum="FC4743FBFD81EE56" modified="2013-10-16" version="1">SVADGIEHFKEGRHTEAFQCLNKALSIDPRNVEGLVARGALYANSSNFHKAINDFETALKLNFYHMNARKYLSETLVALGRNYEDENNFEEASKAYQNCLNINPNHQEAKSSLDNLKLRFDLAIVDNKRSIPKLMTSEEKHFIEKAGDSKGESGKQPYYLSILKNDKSDYDKKVVYFKQVVHYYKHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4Z9</accession>
    <name>T1H4Z9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4Z9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011372-RA">
        <property type="protein sequence ID" value="MESCA011372-PA"/>
        <property type="gene ID" value="MESCA011372"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239657_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011372-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11751" checksum="C5755F5DE41061DC" modified="2013-10-16" version="1">MLCQQSKVSIAPDPSSYLLKSFELHKVDDTEALQQNQLLLQIPTPTTKSSISQILWQFGRILPAFGTFCANRGSTGKRSQNKIHYLHTEAPRNRTNITLGSHRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H578</accession>
    <name>T1H578_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02113294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H578"/>
    <dbReference type="STRING" id="36166.T1H578"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011453-RA">
        <property type="protein sequence ID" value="MESCA011453-PA"/>
        <property type="gene ID" value="MESCA011453"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707619_0_0_1"/>
    <dbReference type="OMA" id="CEHELMF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8465" checksum="26CEC3CAFD4ABD0A" modified="2013-10-16" version="1">MSVSSMEKHLFNLKFAVKELERSSKKCEKDEKLEKAKAKKAIQKGNMDVARIHAENAIRQKNQSLNYLRMSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5T7</accession>
    <name>T1H5T7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pleiotrophin/Midkine C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pleiotrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5T7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012070-RA">
        <property type="protein sequence ID" value="MESCA012070-PA"/>
        <property type="gene ID" value="MESCA012070"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121104_0_0_1"/>
    <dbReference type="OMA" id="VKGQWSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048332">
        <property type="term" value="P:mesoderm morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.90.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.90.10">
        <property type="entry name" value="Heparin-binding Growth Factor, Midkine, Chain A- C-terminal Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020090">
        <property type="entry name" value="PTN/MK_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038130">
        <property type="entry name" value="PTN/MK_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020091">
        <property type="entry name" value="PTN/MK_diS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21050">
        <property type="entry name" value="MIDKINE AND PLEIOTROPHIN 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21050:SF1">
        <property type="entry name" value="MIDKINE AND PLEIOTROPHIN 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01091">
        <property type="entry name" value="PTN_MK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57288">
        <property type="entry name" value="Midkine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pleiotrophin/Midkine C-terminal" evidence="7">
        <location>
            <begin position="47"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012070-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="152" mass="17070" checksum="1C796775E5C38F2B" modified="2013-10-16" version="1">TADVQETPLALPVAEQTQPTTSAQGEIWEEDDHEILIRNERGTKSDGLSCRYGKNPWSECDLKTNTRSRTLTLKKGDTGCDATRTIQKKCKKACRYEKGSWSECTTGQMTRSDKLKPTSDPSCEATRIIKKNCKPGKSKDKSAKEQRKNKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6B1</accession>
    <name>T1H6B1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcium channel flower</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Homomultimer. Associates with the dally/ magu complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the calcium channel flower family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="MESCA012244-RA">
        <property type="protein sequence ID" value="MESCA012244-PA"/>
        <property type="gene ID" value="MESCA012244"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654151_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10233">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012244-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="17057" checksum="847B56372F78F319" modified="2013-10-16" version="1">MAIEAPVCCMFIEHVKMVTDKIESRPLWNKAALYCVAAIPPIIICPGLGSIFGCGLVFATGILYGMMSLGKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASAAEMRSAAVNSTIRQVTTNDRAGIVSNAQPFSFTGAVGTDSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6F1</accession>
    <name>T1H6F1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="MESCA012284-RA">
        <property type="protein sequence ID" value="MESCA012284-PA"/>
        <property type="gene ID" value="MESCA012284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF7">
        <property type="entry name" value="NADB-LER2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20430" checksum="6C56F18540C000FD" modified="2025-10-08" version="2">MNNFDKYNDLVKEIDQVVQDEGLNLLINNAGIAPKSQIIFSKKDDMISTFITNTVAPTMLSKXXXXXXXXXXXXXXXXXXXXXXXXXXXNMSSVLGSIEKNVEGGVIAYRASKAALNIVTKSMSKDLKESSIIAVAIHPGWVRTSMGGSNAPLDVDTSTENIVKTLLSLDKSHNGGFYQYDGQKLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6L6</accession>
    <name>T1H6L6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6L6"/>
    <dbReference type="STRING" id="36166.T1H6L6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012349-RA">
        <property type="protein sequence ID" value="MESCA012349-PA"/>
        <property type="gene ID" value="MESCA012349"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3074724_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
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        <property type="entry name" value="RBD_domain_sf"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN BOULE"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012349-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="53" mass="5903" checksum="20F00E544BFD6A04" modified="2013-10-16" version="1">NDTTETELCRVFSAYGNVKSTKIIVDRAGVSKGYGFVTFETEQEAQRLQNDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6X3</accession>
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        <recommendedName>
            <fullName evidence="1">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
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                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA012455"/>
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        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="IP5"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
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    </dbReference>
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        <property type="entry name" value="LD06095P"/>
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    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
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    </dbReference>
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            <begin position="201"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="322" mass="37032" checksum="7FD7B193B8173218" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYVFWFGDLNFRLAGNDSPEEVWKKTNSRAERDQLVVRREKKAFEYLEERLPEFPPTFKFKEGTSIYEYNFRRRPAWTDRILYNVQKNNYANVTLELDQKSYKCHPGYNISDHKPVTSEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA60</accession>
    <name>T1GA60_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000106-RA">
        <property type="protein sequence ID" value="MESCA000106-PA"/>
        <property type="gene ID" value="MESCA000106"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087555_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000106-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13450" checksum="34A3C50B39F118CB" modified="2013-10-16" version="1">MLEQSPSSAGEQLQKTMKYSCVVHNIDIEIVHDFPKSATVKDLSERGEFEALRYDGSENFTVNRKEVDTSIVLQLHLSYLPTVEVENTLQVPLIFFVTELDIHKEYSQYPRLVLTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAT6</accession>
    <name>T1GAT6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAT6"/>
    <dbReference type="STRING" id="36166.T1GAT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000347-RA">
        <property type="protein sequence ID" value="MESCA000347-PA"/>
        <property type="gene ID" value="MESCA000347"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1572424_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000347-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19371" checksum="CC4C70073FF2072D" modified="2013-10-16" version="1">MVVGTYRIRVPDAAEVGGFTYRIPHCGCKPANLGEISGFRFQIVTRRSVDCLLLAATFRKPPNLSSETDPQLTTHKDYSIIRDTLYESVAAMLQASIEKKRNFLETLELKIGLKNYDHQRTIVSQEPSTFLEIYNILMKLSRTTTYAEALNKWNKNKKLVKNLANILMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBG2</accession>
    <name>T1GBG2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000594-RA">
        <property type="protein sequence ID" value="MESCA000594-PA"/>
        <property type="gene ID" value="MESCA000594"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877780_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577191" evidence="2">
        <location>
            <begin position="20"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000594-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15769" checksum="3BA305E0D0FAFC9F" modified="2013-10-16" version="1">MTEIAFIIFVIFASTFVWTHPPAPATSHKNCKELQYLDKEAYNDFKNYSSLFESEGLGKLEDYFGKVRCRRTTTRKPETTTKTTENPTTTTTTTPNILSPHFLQLQNQQQRQPLAVQQRRQLLSLQLQQLIEDSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBQ6</accession>
    <name>T1GBQ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Polyprenal reductase</fullName>
            <ecNumber evidence="2 9">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8 9">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.94" evidence="2 9"/>
    <dbReference type="EMBL" id="CAQQ02128611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBQ6"/>
    <dbReference type="STRING" id="36166.T1GBQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000693-RA">
        <property type="protein sequence ID" value="MESCA000693-PA"/>
        <property type="gene ID" value="MESCA000693"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375274_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="120"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000693-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="23366" checksum="A89CDA9F3C7C76F1" modified="2013-10-16" version="1">MLALIESLHINLVDFMFINFSMMIVFAGALMNFLEPHLPQFITQSFRYGKHESKGEKNPLVCLLEVPKSWFKHFYVFALGWSALALYLTVKGIIFRTSAPEFVLRTHGQDFSFRNLRMGQIICCILFHLVWREQYKSNMIFVNLRKDKSGKQVTEDHLIPSGRLFHLITSPHFLCEILMYVLIFCIIPQSSSWMYCTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC50</accession>
    <name>T1GC50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02083172">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC50"/>
    <dbReference type="STRING" id="36166.T1GC50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000848-RA">
        <property type="protein sequence ID" value="MESCA000848-PA"/>
        <property type="gene ID" value="MESCA000848"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_30_1"/>
    <dbReference type="OMA" id="HEGTIKI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000848-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15856" checksum="D13715AA5EAB6A5E" modified="2013-10-16" version="1">VELREHEHTVECIAWAPDTATAAINEAAGADNKKGHHQGPFLASGSRDKTIRIWDVGVGLCLFTLSGHDNWIRGISFHPGGKFMVTASDDKTIRVWDLRNKRCTKTLYAHQHFCTSIDFHKTHPYVISGSVDQTVKVWECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCJ5</accession>
    <name>T1GCJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">carbonic anhydrase</fullName>
            <ecNumber evidence="2">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 11">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02033553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCJ5"/>
    <dbReference type="STRING" id="36166.T1GCJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001010-RA">
        <property type="protein sequence ID" value="MESCA001010-PA"/>
        <property type="gene ID" value="MESCA001010"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039326_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00326">
        <property type="entry name" value="alpha_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF265">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588191" description="carbonic anhydrase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="8">
        <location>
            <begin position="15"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="30819" checksum="FCAAAFAA1018D002" modified="2013-10-16" version="1">MMKAFAIISFVISVSAWNYKDQDTWSQEYSQCNGLQQSPIDLLIESTIEKDSIPIEFIRYNKIFKGYDMILENNGRTIQLSFSENIPDEFRPSIIGGILGNETFILDQIHFHWGSGTGRGAEHTFNSKRYELEVHLVHRNSKYDSVEGAAGEHNGLTVLGILGGFREISDCLQFIRYSGNSTSLIYNFNPGKAFPVDKMEGFFNYVGSLTTPECSENVNWIVFNDVQRISEIELEKFRSLKTEDGSDLMDNWRVGQDLNGRNVYFIKNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD24</accession>
    <name>T1GD24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dynein heavy chain AAA module D4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD24"/>
    <dbReference type="STRING" id="36166.T1GD24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001203-RA">
        <property type="protein sequence ID" value="MESCA001203-PA"/>
        <property type="gene ID" value="MESCA001203"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_272808_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF22">
        <property type="entry name" value="DYNEIN CYTOPLASMIC 2 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="4">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="2">
        <location>
            <begin position="287"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="5">
        <location>
            <begin position="434"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein 2 heavy chain 1 cytoplasmic ATPase lid" evidence="6">
        <location>
            <begin position="757"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="3">
        <location>
            <begin position="874"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1183" mass="136495" checksum="F9F7CCA463DF32E6" modified="2013-10-16" version="1">MNNKKMLDIGSKWVDFNENFRIVLITKSTKIDIPEETKAYLTLIQFTTTLIGLTDQLISKTILHKNPKLEEKRVELLKMEGSLLQQKIDMEDNLLEELSNSQGDILKNEKLLRTLNEVKESSLKIDKSLQESFNLKSALEEEYKQYRDICSKAASLFLGINRKYELSSIVFSNLYLKTLEMKLDDLESYRFFVKSVFLYLCRSTTKEEHIILGTFICKNAFPDQIPLKKWELFVTNFMVTRDGESNYSIPAWVNKDYVSKVMALISQVPKLEEGGGPRRLSEEGGNSKPILLITEPENDPSDEIRNIAAKKLGSSDNYIELSVGKGMEKTLLELLRKHSTSSKWICFKNIHLMPLWLIDLDREFEMLKKAENFRMWLICEHTRNFPETVTYKFINILFEYPNGIKNKSKRFIQQEYGASLTKMIKDARSTKINILLYILTAVLQERRKYIPQGWSQKYEFGNADLKAAQNIVGWLENSFNSSKMDWSVLQNLCHSVAYGGRMNNSRDLEVLKKYLKLFFNNECLSNKWSPIDLGVNIPTTGNIQDVYNESRDAVEFYESDNSNSPNQQLLHTAQIKVYLDVIKMFLENLTPFIICGPSAAAKSLLIEEAVKEFSGYQLVDIHCSMQLTPSYILHILKQNSLTVSGIKGREYKPKQFRLILFFRNLDLCDIDSWGTSDVVELLLSLLEKQGFYNESLEWITVSGIHICASISESLLTTKKLSPRFMCRVGHLRIKHPSDSDMEIVLTNSLQPILVKFKKSYTTDVVESLTAFFRKVQESFMGKGESSHYHFTPKTLIKLINGLYNYPEDNFNEALLCEITSIFKDRLANQEDITIYNDIFRETMRRFLWVEKEKIFFIPKQSNIESFEALDPVTKELLDQTAKITRALSRPKNHLVILGEPGSRYLDSIYISASMYQAKVSIPQMTRNYSLLEFYNDLKIAMTSAAIDNQIVCMLLEQSLISFQPDVMKPIEAIMEESEIIDLFGEDLDSIANQLKSAAQLEGFQDSLSSFFMSRVQQNLHLIVVLNPKSSNTSKFFSQYPTFRRNAEFLYIRNMSEETVLSLPKMVIESQSEKVPISSYFTEILTNQYNKQPPKLAYQMINCYYYIYKSYNGNIQRNLHKLQTGVDKLASAHKVVGELKASALKQEESLAEKRKMANEALEKISSTMRNANEQKSTMLELRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD35</accession>
    <name>T1GD35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA001214-RA">
        <property type="protein sequence ID" value="MESCA001214-PA"/>
        <property type="gene ID" value="MESCA001214"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_732104_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21974">
        <property type="entry name" value="RE15880P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21974:SF2">
        <property type="entry name" value="RE15880P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001214-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="364" mass="40661" checksum="7615B5D6D6FA3384" modified="2013-10-16" version="1">MCPGPRLATAEAWVDHLQMKLNTLQESIPAVPKANGTVQLHNNENGGSSSSTINVLSNSKSLPNGINSHYIDQTTDIARLALNLSVLEDVQKASSNEDFFAKLSQTSLDEMTIKFYEEIIDHAKDRAKGLLAAFTQLSDLYVEQDGIIASISGGTYLTTFEEATDAELEGARDVRDKLGSAKXXXXXXXXXXXXXXXXXXXXXXXXXQWRTCAVLLKASSKSAESILSMWTDVNNARLPRDKIGKVLDCRQMIQASLISLECAQLAIPHIEIKHLSNRQILAVKHANTYLVTDVADKNRFEHTYKILEKYIESINKASEWIYETFNKTLKVDFDKAEATVQTLSETLREHRVELFKGKLPRRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDJ0</accession>
    <name>T1GDJ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001378-RA">
        <property type="protein sequence ID" value="MESCA001378-PA"/>
        <property type="gene ID" value="MESCA001378"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239678_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576833" evidence="2">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001378-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12431" checksum="9637C8ADA3FDD958" modified="2013-10-16" version="1">MIWWKVFLAAIFYMEGFVAQRSHCDKQCGRICMIELVCLRDKLDDKTLMFYESCEPCRLRCLYPYRYSKNKICSSVNSNNSNIQSIKTTKNNPKNSYKSLSKNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDW2</accession>
    <name>T1GDW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl hydrolase family 31 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02060724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDW2"/>
    <dbReference type="STRING" id="36166.T1GDW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001513-RA">
        <property type="protein sequence ID" value="MESCA001513-PA"/>
        <property type="gene ID" value="MESCA001513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944328_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF4">
        <property type="entry name" value="MYOGENESIS-REGULATING GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6852" checksum="837A0BC19767B088" modified="2013-10-16" version="1">MLGDVFLVAPVIVQGQTERDIYLPKGEWVDGNNVNVVHIGPKWIMSYPAPLRKLPYFQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEY0</accession>
    <name>T1GEY0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue found in the consensus sequence C-X-S-X-[PA]-C in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEY0"/>
    <dbReference type="STRING" id="36166.T1GEY0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001904-RA">
        <property type="protein sequence ID" value="MESCA001904-PA"/>
        <property type="gene ID" value="MESCA001904"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041919_1_0_1"/>
    <dbReference type="OMA" id="EDDCMFP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035252">
        <property type="term" value="F:UDP-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045747">
        <property type="term" value="P:positive regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="7">
        <location>
            <begin position="146"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001904-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="400" mass="46661" checksum="20F398F7DEAFEBCD" modified="2013-10-16" version="1">MNAFLCTKVPSVLVSSIGLLAARKFVSYPLTPGTSVSVKWCECDSEPESTNLYDKDFNKWLQLISSQNKSYIPCINGKEDCNCFLGQIDKDLQHFQHGIQESQIESAKYRGTKYQIIDHKLYRDSSCMFPSRCSGIEFFLKQTLDHLPDMEFIVNTRDYPQVSKAFNEKLPVFSFSRTNSDLDIMYPAWSFWAGGPAITLHPTGIGRWDKHRNSIAKAAKKFPWSEKESIGYFRGSRTSDERDSIVKLSRKHSDLVDAQYTKNQAWKSKEDTLGRDPASEVSFEEHCRYKYLFNYRGVAASFRFKHLFLCKSLVFHVGNEWQEFFYSDLKPWIHYIPLDSYPSEDDHDELAERIALNGYKQIWSNLKLKDITCYWKNLLEKYSKLIKYDVKKDENLIKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFY9</accession>
    <name>T1GFY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02181628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFY9"/>
    <dbReference type="STRING" id="36166.T1GFY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002292-RA">
        <property type="protein sequence ID" value="MESCA002292-PA"/>
        <property type="gene ID" value="MESCA002292"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1490660_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF135">
        <property type="entry name" value="CLIP DOMAIN-CONTAINING SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002292-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="19555" checksum="2ACDF9D7E0E97AA0" modified="2013-10-16" version="1">MSAALRSSAGTFVVLKSLDFSVLGDATLPPACGGGDSQRTGGNYKPTENRGPSSSDSDLIPEPGVCGNVITNKIYGGVETRLDEYPWMALIEYQKPGNKKGFHCGGSMISNRYVLTASHCINARSIPADWKPISVRLGEWDTATDRDCDAGECAPPHIDVPIERSLVMKIIILMLRINQMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG62</accession>
    <name>T1GG62_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG62"/>
    <dbReference type="STRING" id="36166.T1GG62"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002371-RA">
        <property type="protein sequence ID" value="MESCA002371-PA"/>
        <property type="gene ID" value="MESCA002371"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929976_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002371-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14071" checksum="CAF4AC3A6E127C23" modified="2013-10-16" version="1">MVYYDNFKVTPSTSHQAQQQHHHQHQAHQPQQPSTSSQNTSSSSQLSQNLSPEVLLSLVQAGHLPQDVLLKLVQSGDLHLHTEEGGQYITIPIPVASTSSKATSSNKGNTNRKGGGGNSGSSTFIKKETSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG78</accession>
    <name>T1GG78_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Deltamethrin resistance protein prag01 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002388-RA">
        <property type="protein sequence ID" value="MESCA002388-PA"/>
        <property type="gene ID" value="MESCA002388"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592510_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031973">
        <property type="entry name" value="Deltameth_res_prag01"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16020">
        <property type="entry name" value="Deltameth_res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deltamethrin resistance protein prag01" evidence="2">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002388-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8768" checksum="B58A4D0CD2EB6D1B" modified="2013-10-16" version="1">MSTMSYILSRAVLRRAPVAARSYGGGHGHKVTMNDLPVPQGDWKEAHSRANSKLRPVESSNSTTVHQNPLTKLILRTISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS8</accession>
    <name>T1GGS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGS8"/>
    <dbReference type="STRING" id="36166.T1GGS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002609-RA">
        <property type="protein sequence ID" value="MESCA002609-PA"/>
        <property type="gene ID" value="MESCA002609"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_804851_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002609-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="345" mass="37833" checksum="7E680645A74953EF" modified="2013-10-16" version="1">MSLTGMVDKRTLEKYEREAREKSRESWYLSWALDTNQEERDKAFHNFGAPGHKSFVPNMIAGAAQADLAILVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNWDENRYNECKEKILPYLKKLGFNASKDLTFMPCSGLTGFGLKEQIDDKVCPWYRGPAFIPFIDELPSLNRKADGPFIMPIVDKYKDMGTVVMGKVESGCARKGQNLLVMPNRRKKHLPFSAAAFSSAARFCGTVPGASGEDVFVVLGESLAEFVVGVMGNVEGGVATTGDSSLSVVGGWADAATAVEFSCSWLLLVLFVVEEDRKLGTNSMAFTFNVENLALNSDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGY7</accession>
    <name>T1GGY7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleoporin Nup188 N-terminal subdomain III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGY7"/>
    <dbReference type="STRING" id="36166.T1GGY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002668-RA">
        <property type="protein sequence ID" value="MESCA002668-PA"/>
        <property type="gene ID" value="MESCA002668"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1499975_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="8">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002668-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="180" mass="21071" checksum="330C175789D807F5" modified="2013-10-16" version="1">RVINLNLLPQITNVSLDYKQYSNGVCFDSGLVGMYLVNIEKKNEKYSFVKAYLKFLRTFARLETRNIFQTEIPGLIFLIREVFPHLHTWRFQDEQERNHIYQEIMAFFCDILDGSVKCNQKSILRDICVISLLNLENGLVLLRFIALGNPYLQLLMEMESNWIAPSANTILPLVRFSMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH96</accession>
    <name>T1GH96_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Voltage-gated Na+ ion channel cytoplasmic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02156503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH96"/>
    <dbReference type="STRING" id="36166.T1GH96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002786-RA">
        <property type="protein sequence ID" value="MESCA002786-PA"/>
        <property type="gene ID" value="MESCA002786"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140397_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Voltage-gated Na+ ion channel cytoplasmic" evidence="2">
        <location>
            <begin position="109"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002786-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="244" mass="26381" checksum="12FDCA399A4BDFCB" modified="2013-10-16" version="1">KKAEEEEAAEEEALREAEEAAAAKAAKLEARAAQALADAEAALHPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKISLRSDGLESVGEMQKTHAIYASSTKVRKVSTTSLSLPGSPFNIRRGSRGSHQFTIRNGRGRYGGPVNDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGTILVPMYYGNLGSSTQDINTAYLILFPFLFILKTGSRHSSYTSHQSRISYTSHGDLLGGLAGIGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHF6</accession>
    <name>T1GHF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02037127">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037128">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHF6"/>
    <dbReference type="STRING" id="36166.T1GHF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002853-RA">
        <property type="protein sequence ID" value="MESCA002853-PA"/>
        <property type="gene ID" value="MESCA002853"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1295670_0_0_1"/>
    <dbReference type="OMA" id="RMCEAVI"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="9">
        <location>
            <begin position="58"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002853-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="24305" checksum="5536F098BEF970A5" modified="2013-10-16" version="1">MKLYFNSESRNNSKYFPSLKNANLLPPKEGGLKKDFKMSEMLLKDEGLWFNIFCFFPDLYQITMAYAYWKSGKTDNVAVFDLFFRTNPFHGEFTIFAGLEECLKFLDNFKYSDSDLRNLTAKDITLYAIEEGSVAFPRVPIIKIEGPLIIAQLLETTLLTLVNYASLMATNSARYRMVAGKNIQLLEFGLRRAQGPDGGLSASKYAYIVFKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHN1</accession>
    <name>T1GHN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS4 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02112319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHN1"/>
    <dbReference type="STRING" id="36166.T1GHN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002939-RA">
        <property type="protein sequence ID" value="MESCA002939-PA"/>
        <property type="gene ID" value="MESCA002939"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097281_0_0_1"/>
    <dbReference type="OMA" id="FRIKHEQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002939-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="20918" checksum="EB1BE889B77A7585" modified="2013-10-16" version="1">MVRKLKFHEQKLLKKVDFINWKVDNSAHENKILRRFHIRKKEDYTTYNKLSREVREFVEKIAALDPSDPMKAECTDKLLIKLYNMGVVDNKLDLETASRVSASSFCKRRLPCINKLSENLKNATELIEQGHVRVGTEMIKDPAFLVTRNLEDFVTWVDGSAIKKHVEEYNDMRDDFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIS3</accession>
    <name>T1GIS3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003358-RA">
        <property type="protein sequence ID" value="MESCA003358-PA"/>
        <property type="gene ID" value="MESCA003358"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624848_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003358-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9316" checksum="85B3E28F36D2C0A8" modified="2013-10-16" version="1">MKEKKICFVEKYAFDENDGNSIGFIRNGGQKIFFSFFTLKKKKRNIYNSSFYTGTFLKNNIIFFCFFLFSSKLKMDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ12</accession>
    <name>T1GJ12_MEGSC</name>
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        <recommendedName>
            <fullName evidence="4">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1GJ12"/>
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        <property type="gene ID" value="MESCA003451"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metallopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000158">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
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        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="237"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003451-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="31017" checksum="A6FF6445B9574DCC" modified="2013-10-16" version="1">MNTYRIPFDEDDKDKSVWFLDHDYLESMYAMFKKVNARERVVGWYHTGPKLHQNDIAINELIRRYCPNSVLVIIDAKPKDLGLPTKAYISVEEVHDDGSPTSKTFEHVPSEIGAEEAEEVGVEHLLRDIKDTTIGSLSQKITNQLQGLKGLNAQLKDIKNYLEKVGSGKLPVNHQIVYQLQDIFNLLPDITSEQFTDTMYVKTNDQMLVVYLASMVRSIIALHNLINNKLANRDAEEGIKKDSEKRKRIKMGRKLKKPQKNPRKALRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ72</accession>
    <name>T1GJ72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02167975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GJ72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003516-RA">
        <property type="protein sequence ID" value="MESCA003516-PA"/>
        <property type="gene ID" value="MESCA003516"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_242060_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor HIVEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001151">
        <property type="entry name" value="zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051969">
        <property type="entry name" value="Zinc-finger_DNA-bd_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944:SF2">
        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="494"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
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        <location>
            <begin position="957"/>
            <end position="986"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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        </location>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="1031"/>
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        </location>
    </feature>
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        <location>
            <begin position="1073"/>
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        </location>
    </feature>
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            <begin position="1133"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1344"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1588"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1635"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="592"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="683"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1033"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1086"/>
            <end position="1099"/>
        </location>
    </feature>
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        <location>
            <begin position="1101"/>
            <end position="1110"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="1192"/>
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    </feature>
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        <location>
            <begin position="1353"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1635"/>
            <end position="1658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003516-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1664" mass="186322" checksum="B1EC716EE1355543" modified="2013-10-16" version="1">SATESLNDNLTKTSVEATTTQQRESLESTTVVQPLIQSNGHVNGNSNHILSSSSSPNSTTARTPTPPQTTALSVDTVETSFDGFSKSSSGASAPPPVKDMYNKYKFCPYCDTFFSKPSVLEKHIRTHTNERPYPCEPCNLAFKTKTQQQEERELHKHSKRFKTQQQESPVKQATITPLPIVPQVPLVQHFKFPPINQITAFNPLTLSMVADVADKNIPYVPGIPGPNTLLSIPSVLPPPQNLLRPSPNNAKSISPNKSNNQIESTFNMRLKPIGSSNSHSQSIAWNPVKDKKSFNFARMADNLSPKKSKTELHESEAYNKYYDKQKPELKTLKPLSIDVIQASSSPPSTHVSDDEKKKNKFLRPSSLPLKPGTFTPKRHHGITPTANTLPLISPETPRPSKSCAQMYLNGHAYTYLGLKCSTKMFYCTVNCPQPTYVANNHRLSMYSKWQAVPDSKPHPMKLDQKKNSGGGLLIGGYESNEDYTYIRGRGRGRYVCSECGIRCKKPSMLKKHIRTHTDVRPYTCKYCNFSFKTKGNLTKHMQSKAHYKKCLELGIYKGPMPADSEFPENDNDIDQQSINSGGGRTSSIFGESDSDDFSDNDEESSAAHCLLSLSMTKGTFNSFPQSSCQQNLDTIQALPSKRIVSSYSSPKPPYEFKHDGKYYPKPEVSKSKMIMKSDECTKPIDLTKPKKNNEQPTPINCSKKMPIKPTPVHKPIKYREVIGEISTETGMLKSLVSLTDMVRGTIESSNINTDENILPQNYLTEQALHQSKIKQCQMQQPSTNLTVSINTVKSTAIESLTIPPSSTSSATENAQTSLVINRQNDIFDSLKRGDKQIDSTSNFSNLEKTMEESDDSLSDQELFIPPITSSNSNDFINLFGARKVVVGEEGNFKNPSSNTGDSVQPMFPRTPISEDGKPVCTMTQGHLQKHERSEAHKNKVMMTSTFGVPTTTNPRPFECTDCKIAFRIHGHLAKHLRSKTHVQKLECLQKLPFGTYAEIERAGINLTEIDTTDCENSLIKIKNILKKLTQRVNSSNPSPPLVQQQQQQNQSNELIKPYKPKFHNAALYTSKEEVLKEQHPSKPLSPFQTPNKMVSNHNSPTPTPTPPPQTPQITIFTSNNIQILQQQRIQPDNVYHQKSPSSQQQSQFGQQSFPKHISPQQQQKQLLITSPHNELYQISRPSSTQPSSTISLNRDRMSPNKNLFGNQRILTQQIDVSSPYHVLSKQQQQKHQQQLQTNVSTYTNSKVFRSQDIEVGSNTIAPMNPSTYFQQINEQQKMSNSSQQQDVVDKKRINLVEINNVGNRNDIIKSIIITNLEERINNLITQNEVILENKEPLLKNKKSKTLNRQKSITSMPNSAPPSSSTNTKLAQTITMKQQQQQQYLQAQASTHQQKLIQQVNGFYPQKITARPQVQIVQQMEQQPISKLCLPSTNTSQPLNLSKLNESSPIKNKNDEPVPLNEASSLASAAAAVLARNPERSIIKNLLLAKEQQEKIDRTDSTQAYMCTVCQMAFRSSDELKNHTNYCTSGLNHQSGSRSPRDSPSYKYFNSNTFMSIPDVTNPSSLLTLATSAMSKSIAKLAWSQLSQRTKPTSPALIDTSLPSPGPRLGKTALVESYQPTADIKKNDDVIISKVHNDEISVPQEKQKEQHQQQEEKELHKHSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJG2</accession>
    <name>T1GJG2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003608-RA">
        <property type="protein sequence ID" value="MESCA003608-PA"/>
        <property type="gene ID" value="MESCA003608"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280596_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003608-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="12112" checksum="28AF707A6DB8844E" modified="2013-10-16" version="1">MQCLVRRNWSIATTTKCMHCFPLCEQISYSEQRKPTYNYGGLDFSLTLKILNNKACFVFENIEYTILTKNSQKLVNRASKVDYIEIYFDYIEHCIRGENHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJW6</accession>
    <name>T1GJW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003773-RA">
        <property type="protein sequence ID" value="MESCA003773-PA"/>
        <property type="gene ID" value="MESCA003773"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006515_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003773-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14415" checksum="3C79FC38E2F4913A" modified="2013-10-16" version="1">MDGDGWWILPSKDKRTTKAQVQMAEKKEYMFTSLRTSNQERVGPKINIGHYDFEVANNFIYLGTEFTSDNIRGRKKNHTRQQMYIGLEQTNAFKTHISKNQGQALPPANYASLILWKWEMGSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK23</accession>
    <name>T1GK23_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023332">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK23"/>
    <dbReference type="STRING" id="36166.T1GK23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003837-RA">
        <property type="protein sequence ID" value="MESCA003837-PA"/>
        <property type="gene ID" value="MESCA003837"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999551_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003837-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="279" mass="31397" checksum="772A4EFB9417C690" modified="2013-10-16" version="1">MRPEKSTDGINILKRYYCQLHSLTHRFPKPVKSLFTFTWTDKYSNTTMEYNDINYEMASVLFNIGALHTQVGSGVKRTDVDGMKLACTHFQCAAWCFTELKERYGNLINYEEFMSEELLIFMQQISFAQAQECILEKSLIDNRKPIIVAKVTSQIISYYNTGMSALIAAKDKSEPKSVQQIKKVEEKKPEKSSVLDLLADIDFSEGQAPLQPALIATKIETTRRVSLPSSDSLEGENDTINSSIPIKTAQPPAQTIDRKQSTDNLSICSEVSSIAIDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKC2</accession>
    <name>T1GKC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKC2"/>
    <dbReference type="STRING" id="36166.T1GKC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003943-RA">
        <property type="protein sequence ID" value="MESCA003943-PA"/>
        <property type="gene ID" value="MESCA003943"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_304690_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06745">
        <property type="entry name" value="PDZ_SIPA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000002">
        <property type="entry name" value="Signal-induced proliferation-associated 1-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF22">
        <property type="entry name" value="RAP-GAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="6">
        <location>
            <begin position="242"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="579"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="704"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="769"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="793"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="903"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="721"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003943-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="975" mass="111273" checksum="413DE4B75F9290CE" modified="2013-10-16" version="1">MLQDYRPVVMGVIGTDQRSPGPGDFIKEETSSNATASSNDYDTALNISQDVIERYRRRRFAHYDCQSLVACLKYAAKLKESLEQRQNIVTGASAAHDYKKNFINVTDDVSNELIQSCQYFVNEIGGEIEKDVSLSRVNSLNANLNSGGIIHKPCLAYGVSILDLNVDNNNWKSNTCPCTVQQPKIDFVDHGSKYYRDFFYGKDHQNFLGTDDDLGPVVISVKKEKCNANVDASSCCYQYRQIMKLDEQAITNKYKVGILYCKKGQSTEEEMYNNEQAGPEFMEFLEIIGKKVRLKGFENYKGGLDTKTDSTGLYSLYTSYENNEIMFHVSTMLPFMTNNKQQILRKRHIGNDIVTIIFQEPGSLPFSPKNIRSQFQHVFIIVRAVYQPYSCNTYYQIAVSRSKDVPIFGPPLPNNLFSKNDVFYKFLLTKIINAENAAHKSTKFYTMATRTRQEYLKDLIKNFSSLTPLDTHVLNKFSIFQQKPKKVTTKNTTLNEKIVQTGAYCWDVVLQDQSNGYGSIPVNCLLGISFDTFVLMELKNKQIVGMFEISVAYNFENRDVNVEIIERLKKVTQGCSTVELNLKRDLSGHLGFHVQSDGVVTKVDSNGQAWLSGLRPGYRIVEICKIVVATLSYDQLVDLLKTCDYINITVIESSNESTNLPRTGCYNLNILPRIKEYDKYDISLYTPDKYETDLMTPDRIVKSRKASKQMNRKLNFSPPRSSNSSGYGTSSSSRSFGVNGLSSSVNISKLTTEQNLDLKGGEHKYLKEVKSRPKSIHDPTDIFSENHRKYPFNNSEINGEHSPNHSRVSSNNPTLEKVEVKVNRKKSMNTHLKKHSTFQKDLLNLINPDYEDNSREAQKGYSPEYPAQKIKEEYLYKLNNESVNKSEDCLSVANKAISDGYLTKDENKTMDQLQAHLNSLETQILKDKQRRKSLEDTVQRLSEENRRLQSESEAAVQQLRTFTEWFFQTIDKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKC3</accession>
    <name>T1GKC3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KIF-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKC3"/>
    <dbReference type="STRING" id="36166.T1GKC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003944-RA">
        <property type="protein sequence ID" value="MESCA003944-PA"/>
        <property type="gene ID" value="MESCA003944"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186946_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF2">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D11, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003944-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12928" checksum="706E464341F41127" modified="2013-10-16" version="1">MGKCCKLKTLFFIKFLYQVDDDYSIVHQLSRAILSYLVSMYGKDDALYPADIRTRALVDARIQFDLGTLYARYCDYFKYLKILKANSKSELEMSKYYFTTFKKILSTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKL1</accession>
    <name>T1GKL1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02027736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004037-RA">
        <property type="protein sequence ID" value="MESCA004037-PA"/>
        <property type="gene ID" value="MESCA004037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1082941_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="257" mass="29688" checksum="48701EDFA30559A4" modified="2013-10-16" version="1">MKRCLSTKRNLPEASEETTNLEIYLKTFLSLETVLTLNIHTYDEYEDTEISKMEFVECCELLLITVNNSYSLKSILKWRKTFNHILAKLTIISYSERIPSMTLPLTFSYSQVLFILLGKTAKGIAVANLFTLFTTAEEFIATDPYEVPELYWPALKLGYPGFSIADFPLLIGSEVPTTGLLVYFSKAFQKYYENRRDKHKIKNVHFEKLEIKSDISDFSDGYPLKISKVCFMVPVIDEISQEDFLKKPFQLGFGFYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLA8</accession>
    <name>T1GLA8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Queuine tRNA-ribosyltransferase catalytic subunit 1</fullName>
            <ecNumber evidence="17">2.4.2.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>guanosine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:16633"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:18571"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="EC" id="2.4.2.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a catalytic subunit and an accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB3 subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.64" evidence="17"/>
    <dbReference type="EMBL" id="CAQQ02026328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLA8"/>
    <dbReference type="STRING" id="36166.T1GLA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004307-RA">
        <property type="protein sequence ID" value="MESCA004307-PA"/>
        <property type="gene ID" value="MESCA004307"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022060_1_0_1"/>
    <dbReference type="OMA" id="CTRFSIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012576">
        <property type="entry name" value="NDUFB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08122">
        <property type="entry name" value="NDUF_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9 17" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="12 17" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="18">
        <location>
            <begin position="23"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="17">
        <location>
            <begin position="258"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="17">
        <location>
            <begin position="282"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004307-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="509" mass="56329" checksum="1326084292D2EB2D" modified="2013-10-16" version="1">MTTATATKAPPLAYQVMAKCSTTKARVGNLVLPHHHVDTPVFMPVGTQGTLKGILPDQLISLNCQILLGNTYHLGLRPGTEVLKKVGGLHKFMGWPRNILTDSGGFQMVSLLDLAEITEEGVKFRSPYDESNECMLTPEHSIGIQNVIGADIIMQLDDVVKTTTTGPRVEEAMNRTIRWIDRCLEANERDHDQNIFPIVQGGLNPELRKQCVEHLTKRAVRGFAVGGLSGGESKDDFWRTVHICTDLLPDDKPRYLMGVGFAEDLVVCVSLGIDMFDCVFPTRTARFGCALPIDENCSCSTCKTYTRAYLHNIATVESVSSSLISIHNVAYQLRLMGEMREAIHNNTFPEYVKGFFKTLYPNELPPGWVCDALQAVGIDGTLKQLIVIKKRADKMGGHDHHAAPYTVPKASIYKVEACPELMEVQCALARQGLRDPWLRNEVWRYDPKLHGTHASRVRAFFGRGFLLGVGAFALTVAAEYALGIGDDHGHGDHHGDDHGKIRSFCSSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLB7</accession>
    <name>T1GLB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysosome-associated membrane glycoprotein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLB7"/>
    <dbReference type="STRING" id="36166.T1GLB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004317-RA">
        <property type="protein sequence ID" value="MESCA004317-PA"/>
        <property type="gene ID" value="MESCA004317"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079983_1_0_1"/>
    <dbReference type="OMA" id="EHIDRND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072594">
        <property type="term" value="P:establishment of protein localization to organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002000">
        <property type="entry name" value="Lysosome-assoc_membr_glycop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506:SF40">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18067" checksum="17FB374A04A4250F" modified="2013-10-16" version="1">MLAVSKKCDLLTITPGGERWYINNVELRYHTSNTLFEHIDRNDLNVRLTSSAKQSMYFPTPVGKSFECDEQEIIMFAPMESGDMSGHVAKLFLRNLKLQSFMYKQSGAFGPPFQCTATGSYRDEAAPLYVGTALALATLATIGGYSGFRYFKVKNVQYGSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLQ4</accession>
    <name>T1GLQ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLQ4"/>
    <dbReference type="STRING" id="36166.T1GLQ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004467-RA">
        <property type="protein sequence ID" value="MESCA004467-PA"/>
        <property type="gene ID" value="MESCA004467"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006332_7_1_1"/>
    <dbReference type="OMA" id="LAHESYF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032506">
        <property type="entry name" value="SGSH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF33">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16347">
        <property type="entry name" value="SGSH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="N-sulphoglucosamine sulphohydrolase C-terminal" evidence="4">
        <location>
            <begin position="172"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004467-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29412" checksum="73180065A930B971" modified="2013-10-16" version="1">MLKELESFGFDKDTFVMYTSDNGPPFPGGRTNFYEAGIKEPMIIFSPENGVRRNEVTASMTSLLDIFPTVLDVFKISTNTTKLTGKSLLPLLVQEPPRNDNDAIFGSQTFHEVTMSYPMRMIRTRRLKLIHNINYNSYFPVDQDLYTSPTFQEILDSILNKTYLPWARDLKSYYQRDEWQLFDTKIDPMEKTNLVQKPKYKKIFEDLKERLMNWQKATHDPWRCAPHGVLQEQGIFKKDPKCFTLGHEVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM03</accession>
    <name>T1GM03_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004571-RA">
        <property type="protein sequence ID" value="MESCA004571-PA"/>
        <property type="gene ID" value="MESCA004571"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429580_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004571-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10073" checksum="30170BDC84F492B3" modified="2013-10-16" version="1">MAAVSLEILYSTIAFVSHSITDSRTCWLSWRGKVSTLEECATLSSVVCDGSHRLSLKQAVLLGVHSALISVNNLANHFEKTYKLSSYIQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM07</accession>
    <name>T1GM07_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004575-RA">
        <property type="protein sequence ID" value="MESCA004575-PA"/>
        <property type="gene ID" value="MESCA004575"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471641_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004575-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9949" checksum="E0DBD44B9A4DF987" modified="2013-10-16" version="1">MFDRKTKILTRKPTHFELTKNINVDIRSIIYNSNTFVRLSGVGSMVDLGLKRKTLERALLDERPSGTDTEAIETCGEGLTDYEVAAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMF9</accession>
    <name>T1GMF9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMF9"/>
    <dbReference type="STRING" id="36166.T1GMF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004733-RA">
        <property type="protein sequence ID" value="MESCA004733-PA"/>
        <property type="gene ID" value="MESCA004733"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059870_1_0_1"/>
    <dbReference type="OMA" id="GIHEYQD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000043">
        <property type="entry name" value="Syntenin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051230">
        <property type="entry name" value="APP-Binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345:SF3">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345">
        <property type="entry name" value="SYNTENIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="51"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="135"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004733-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="236" mass="25494" checksum="DA2D006D87C38FE9" modified="2013-10-16" version="1">MIRANMPEFAEGALILSPNGSAPSGNAGVVAPLSGNSVVPARAEVSHNIREFILCKDAKGKVGMRVQAKDNGVFVCIVVKGSPAALAGIRFGDQILSLNGTLVSGFSVDKIHSMLKKSDKNNISVVIRDRPFERTITLMKDSHGNLGFQFKNGKITAIVKDSSAAKNGVLINHNLLEVNGQTVVALKDKEITKIIQESPENFVKITIVPTFIYEHLTKKLSTSFLRGIMDHSIPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMK4</accession>
    <name>T1GMK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004779-RA">
        <property type="protein sequence ID" value="MESCA004779-PA"/>
        <property type="gene ID" value="MESCA004779"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760694_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004779-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7983" checksum="18ED94BAD38CB257" modified="2013-10-16" version="1">MELGSLRIANSLLYFGMISLNDTFLQVVRDIFENQAHHILVVKFAQDRTTATIEIRFILILVIPTQPPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GML2</accession>
    <name>T1GML2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02089426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GML2"/>
    <dbReference type="STRING" id="36166.T1GML2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004788-RA">
        <property type="protein sequence ID" value="MESCA004788-PA"/>
        <property type="gene ID" value="MESCA004788"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1837382_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF326">
        <property type="entry name" value="ACID-SENSITIVE TWO PORE DOMAIN K+ CHANNEL DTASK-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="9">
        <location>
            <begin position="42"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004788-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="15702" checksum="77A69BB537C57953" modified="2013-10-16" version="1">MELYLHAPSNYVIQNVKISLHCRQTAASELDLICVVTTLSSLTIAGGAAAFSKFENWSYFDSVYYCFITLTTIGFGDMVALQKDNALNRKPEYVMFALIFILFGLAIVAASLNLLVLRFVTMNTEDERRDEAQAIQVIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMN2</accession>
    <name>T1GMN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154545">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GMN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004809-RA">
        <property type="protein sequence ID" value="MESCA004809-PA"/>
        <property type="gene ID" value="MESCA004809"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412100_0_0_1"/>
    <dbReference type="OMA" id="REDECYL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004809-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21888" checksum="A2A5B2EFE2F00E80" modified="2013-10-16" version="1">MSVLPITHLFHIPFQQVFFIYTYFCSDQPSGRFRLVDKVSPGASILSCXFYNNSIDTTKSWTDDLPKCKHTGVGSTGNNYTTSQNPTKITDRFFNCEISENTCLYVIFSGHNGSEVADLAMQHMPAEIIFDQLRDKNTDEEIREVLKQSFVAVEKSYQNSIDSILAERTSLQCNIAENQGNKQETLSKLTHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMQ6</accession>
    <name>T1GMQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004834-RA">
        <property type="protein sequence ID" value="MESCA004834-PA"/>
        <property type="gene ID" value="MESCA004834"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1572429_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004834-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19218" checksum="BA96975D3EA3793A" modified="2013-10-16" version="1">MSDESDEPDLAYEKTDEVILRPRFKKTTLELPLETIHQKQPLRNVQSVPHLKSLVVENDNKVFTISHAESLSRISMTPEVPSISFANLPKNYEDTPTIEKFRNSTSLYSIQTNNEAKATEKNELSMPRYYRKSGLVGSPSSENFKTISEESIAVSSSSRKHLVEKAKGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNK3</accession>
    <name>T1GNK3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNK3"/>
    <dbReference type="STRING" id="36166.T1GNK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005157-RA">
        <property type="protein sequence ID" value="MESCA005157-PA"/>
        <property type="gene ID" value="MESCA005157"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152069_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005157-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="13510" checksum="FD66EC084C06BB45" modified="2013-10-16" version="1">MTENYENDTIVFAKRLSESNDLQQQHQQQQNQNQMICNERAKEYIRELNAERNRMDKKFPIADRLIEIEIERVQATGRIPQSSREQKYADIYREKPLRITQKVLVPIREHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNX0</accession>
    <name>T1GNX0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02062048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02062049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02062050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02062051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNX0"/>
    <dbReference type="STRING" id="36166.T1GNX0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005282-RA">
        <property type="protein sequence ID" value="MESCA005282-PA"/>
        <property type="gene ID" value="MESCA005282"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010513_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005118">
        <property type="term" value="F:sevenless binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002956">
        <property type="entry name" value="Bride_of_7less"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01223">
        <property type="entry name" value="BRIDEOF7LESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="8">
        <location>
            <begin position="228"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="533"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005282-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="608" mass="68245" checksum="D16D780F4A8BF188" modified="2013-10-16" version="1">MDAVKEEKICLMHFKSSSTLYVLLNDESIKEFKEEGQVFAVLDGGMEGDIIKDLPNNSYIFHESSIELPTSDVNATNELQLSGKLIYISHVLSMARPLIDLTEQIDNALKTKCKLIQGSYYLETCIAFLAFTKTWEPVYLTTYMDVIETLDLNELKQHMNFNIVKNVIDENDQFRKSYDQFINSLPLRQRSVEQVLKFGDIYKCVSSNPNHPYYFNGESVMHWGLKGEIWVAAGLTSATLGIIVTLAILVFIVVRISVGDVFEGNPIGTIMLLLSLVLLFASFIPFAIEYTADHKNSQVTLQDTQIRNTLCAVKVFLVTLCYSITFSIVLCRSIMLASIGSEGGFLSHVNGYIQTIICVFSTLVQLGLSTQLLIVMQLSTQNLGDSVQCDSILYGNWLWGLLAYDWMLLLFWVGLIPFIHKSQRNYREGILLVIGAILCFIIWVTWSVLCQFGYEWREASIPLGAQSIGWALLIGVLIPRTFFIIRGIERSDMAQALPSLTSLSFGQTNNQYAAKCHECVNPAMRSQDSGMCEMEQSPSEAPTLPLRGGHNTNRRPIGFIAHTNVPPPPNKQKQVRKQKMMDSPRSSQTSLELTPPGEGEGNNKVTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP09</accession>
    <name>T1GP09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02198713">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP09"/>
    <dbReference type="STRING" id="36166.T1GP09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005323-RA">
        <property type="protein sequence ID" value="MESCA005323-PA"/>
        <property type="gene ID" value="MESCA005323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003154_0_0_1"/>
    <dbReference type="OMA" id="EIAIAKW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="10"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="32047" checksum="E45C709941C37A6C" modified="2013-10-16" version="1">MTPLEPETIENIKEHDDSMILRKREQTNKKSSESSHSESESEASRSSTGKDFEIEYAGPGEQPEVEKPSKDEYKVAKWMKKNIKTKKTKFLSHNVEYFSAGKTLDALMNSKKVQVHPDSKQKNVEKDLPKEDKSEKTKSDSENVNVQSNEEPSKTKEKRKRKIRLDMHSEQVFVDGSEAYVWIYDPIPIHYWIFGLILLLGAILICLFPLWPPLLRKGVYYLSVAAAGFLVFILALVVIRFIVFITIYLGSLTKLHFWILPNLTEDVGFLASFWPLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPH5</accession>
    <name>T1GPH5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPH5"/>
    <dbReference type="STRING" id="36166.T1GPH5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005505-RA">
        <property type="protein sequence ID" value="MESCA005505-PA"/>
        <property type="gene ID" value="MESCA005505"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770005_0_0_1"/>
    <dbReference type="OMA" id="HGFANKW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018788">
        <property type="entry name" value="Proteasome_assmbl_chp_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053720">
        <property type="entry name" value="Psm_Assembly_Chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31051">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31051:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10178">
        <property type="entry name" value="PAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005505-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="13843" checksum="49CEDF8D613E9CEA" modified="2013-10-16" version="1">MNSPGFDSQLCAKIADVSTEFVVQNFKNQIFIIISQYGKMGNLYSVKFDKVEDNFTAPAKVGIGIPITINCNFGPDSDEVRAALTFLVNKTKLGEMQKEFLISFGLKEINGKVLRELASVLEKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPX9</accession>
    <name>T1GPX9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005675-RA">
        <property type="protein sequence ID" value="MESCA005675-PA"/>
        <property type="gene ID" value="MESCA005675"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963421_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005675-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="7159" checksum="E666EA5C26B072DF" modified="2013-10-16" version="1">MRQRSSSQKYPNFPEQSSKEHKQGFFVENGSLDFSILPKKSRFEEEFLNEVRRPLQYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQB7</accession>
    <name>T1GQB7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine decarboxylase antizyme</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with ODC1 and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02049595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQB7"/>
    <dbReference type="STRING" id="36166.T1GQB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005821-RA">
        <property type="protein sequence ID" value="MESCA005821-PA"/>
        <property type="gene ID" value="MESCA005821"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1437842_0_0_1"/>
    <dbReference type="OMA" id="NTVIRWE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF10">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0688">Ribosomal frameshifting</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005821-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="20816" checksum="550FD43C5D06943E" modified="2013-10-16" version="1">IQQSQVSVDSSLSNNSSDFSDINFAESTRDDLSTCSSDSYSSSGSSGSSTDGVAVVAGDGSALCENVVRKITQSKQPLRVEIKLLLCNNKFTLWETVLNPLNNTLYIAIPPNFAPELSKHSFLSLLEFAETKLDVDAVVLIMKKHQPNRSQLAKTFLVLGFHQLENDSSYVPTYTNIDDCMCFIYNIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQC0</accession>
    <name>T1GQC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02390806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQC0"/>
    <dbReference type="STRING" id="36166.T1GQC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005824-RA">
        <property type="protein sequence ID" value="MESCA005824-PA"/>
        <property type="gene ID" value="MESCA005824"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_418138_0_0_1"/>
    <dbReference type="OMA" id="QRSIMHY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF814">
        <property type="entry name" value="MEPRIN A SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147028" description="Metalloendopeptidase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="143"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="273"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="481"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="579"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="578"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005824-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="656" mass="74013" checksum="E5380650D80E48DB" modified="2013-10-16" version="1">MVLCVIMNKLCVFLAILALAHAFPTREQDPELAGGFFEGDLDLNKDQLKQLQSPISGRNGLINLEKRWPDNKVYFKVTEDLDDAHYNHIIKGLRIIESLQHRMPLQGRIPKQRSTIELPTLSIRFWVLQNWNHHPRIPSCSWIHHQQSTHDRDQFVRIATENIEKGKEGNFNLYDETVVTDFGIRYDYGSVLHYSQYAFSKNGEKTIIPLYDEGNDIGQRKGMIIMKLVNLVVIAAVCCKVALAFPTINQDPELTGGYYEGDMILSNDLQGRNGLRDLSARWPDKTVYFTIADDIDDAHYNHIIRGIRNLEAISCIRFLPADSSTRGYINVTANPGGCFSAVGYTGKVQPLNLELGPLDAGCYRQSASDRDDFVTIVEDNIIPSTKGNFNKYDESYITDFGIEYDYGSVMHYGPYAFTQNGEMTIVPKKEGVEIDMLRFGLVALTIFAVAYGFPTGSNVEDPELTAGYFQGDMKMKIAGRNGLRDPIDHWPGGIVYFNVSDSIDDAHYNHIIKGLRIIESVSCIRFLPATAKTQGYIYVTAEEGGCYSEVGFLNEPQQLNLAMAALDSGCYRIGTIVHEFLHALGFFHQQSATDRDDYVTIVEENLIPELAYNFEKYNDTYITDLESNTIMEVSCITHQSLSVLMDFQLLSLSKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD9</accession>
    <name>T1GQD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005843-RA">
        <property type="protein sequence ID" value="MESCA005843-PA"/>
        <property type="gene ID" value="MESCA005843"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530072_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="21"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005843-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="10145" checksum="051510B39B037DDE" modified="2013-10-16" version="1">MGDNLLNKYDDYFKKAKHPVDRWQYLQKLHQKFCVSWKKEYLHRLNTRSKWKKEEIAYETGDLKIVTVTESKVTMDCMNSGRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQH1</accession>
    <name>T1GQH1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02120107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQH1"/>
    <dbReference type="STRING" id="36166.T1GQH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005878-RA">
        <property type="protein sequence ID" value="MESCA005878-PA"/>
        <property type="gene ID" value="MESCA005878"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_2_1_1"/>
    <dbReference type="OMA" id="MIVFTIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="10">
        <location>
            <begin position="11"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005878-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="316" mass="36067" checksum="D4277567C6B0A0A4" modified="2013-10-16" version="1">MCNSFDYKIHKLKDFPLLESALFVLMSYSTFLIAEVTELTGVVAVLFCGICQAHYTYNNLSNDSRLRTKQLFELLNFLAENFIFSYIGVSMFTFPKHHFDPVFITGGFMCAAIGRAVNVYPFSWLLNLNRKPKISKNMQHMLFFAGLRGAMSFALAIRNTVSEARQTMLTTTSLIVIFTVVVQGGATNFLLNWLNIPFGIEDENEFLNANQYRNEGDYEGGTTRKNDKALVARIWGDFDKSYMKPLLTHSRPTLLDTMPVYCNPLARILTTTEQLTQDGINRNSDTDSEIYQINDSLQNRRDSLNRATCFEFEDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQH6</accession>
    <name>T1GQH6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02395053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQH6"/>
    <dbReference type="STRING" id="36166.T1GQH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005883-RA">
        <property type="protein sequence ID" value="MESCA005883-PA"/>
        <property type="gene ID" value="MESCA005883"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_984443_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588468" evidence="2">
        <location>
            <begin position="24"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005883-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="283" mass="30123" checksum="E1C48B5BE27E7F04" modified="2013-10-16" version="1">MLYFKVFIIGILLVAGLNAECSGRRKCPPGEAGKPGNVGDVGDKGMKGEKGDPGKDGISGLPGSPGNRGPQGDKGEKGNMGQSNMDTNKLEEELLKIFKNDSKGSFLDELLDRINEISKDNGEIGDKGDEGDCNCNDGSIVFPDDSTTAYPKYKSSSCKWNSRKCRTGMAGEPGMKGNQGASGPKGDSGDAGDNGKSGQPGHPGQSGQKGETGVRGDKGDVKKMDKELLRKTIIELMEKNPKFKEMFLKSVEKHITWRGAKGSQGAKGEKGYCPDIDIRQNFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQK4</accession>
    <name>T1GQK4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pro-resilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQK4"/>
    <dbReference type="STRING" id="36166.T1GQK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005913-RA">
        <property type="protein sequence ID" value="MESCA005913-PA"/>
        <property type="gene ID" value="MESCA005913"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073946_1_0_1"/>
    <dbReference type="OMA" id="WTHAEPP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF98">
        <property type="entry name" value="CUTICULAR PROTEIN 56F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005913-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="21592" checksum="DC36B373FD74E999" modified="2013-10-16" version="1">VLLAAICSAEPPLPSNQYLPPSEPSNEYLPPVQTYQAPSNNYLPPQQNARRQPPAQKPSNTYLAPDVTSAIFPNQGNNGYPAARNGNGLGGNGNGNGNGNGNGHHQNDEDSWGPAKYEFKYDVQDDESGNNFGHMEQRDGDLTTGRYYVLLPDGRTQIVEYEADGNGYRPTITYEGVANNGGNGNGNGFNGGSNGFNNGAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS7</accession>
    <name>T1GQS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS7"/>
    <dbReference type="STRING" id="36166.T1GQS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005992-RA">
        <property type="protein sequence ID" value="MESCA005992-PA"/>
        <property type="gene ID" value="MESCA005992"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629429_0_0_1"/>
    <dbReference type="OMA" id="NAMNINQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016698">
        <property type="entry name" value="Numb/numb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368">
        <property type="entry name" value="NUMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368:SF2">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="105"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005992-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="436" mass="48454" checksum="C3EAA68997EBDEBD" modified="2013-10-16" version="1">MLKSIKIKFHKIMGNSSSHTHEPLERGFTRGGKFGDVKNSKAASFRFSSKKSPKKMERLRRSFEIPSEGKGIEYLSHQSHISGVLMNKQCGPIRESKRRPVRGLLHVSGDGLRVVDDDTKGLIVDQTIEKVSFCAPDRNHERGFSYICRDGTTRRWMLHGFLAIRDSGERLSHAVGCAFAVCLERKQRRDKECGVTMTFDVKNSTFTRQGSFRQTTMTERLQMNERGVDTGFVAPQGPSSVSKPFNPFAIERPHATPSIWRGKDHSGHLVQLVLNPHSKDKCLCVLMIFHQMLNGRRQVSPPQPSKQALTPVSPVDTVSQLCQELSQGLSILTHKDEILDDMNFNNAMNINQNILAAELRYTETQSPTTPTKSPSNGYSSMYEKTPPTAEVSPQSATHASSPSTPITSTPISCLADAQTPDPLPSTPTTTKPRTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRA6</accession>
    <name>T1GRA6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sphingomyelin synthase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02120220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRA6"/>
    <dbReference type="STRING" id="36166.T1GRA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006184-RA">
        <property type="protein sequence ID" value="MESCA006184-PA"/>
        <property type="gene ID" value="MESCA006184"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1379557_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006686">
        <property type="term" value="P:sphingomyelin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF27">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:CERAMIDE CHOLINEPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="10">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006184-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22447" checksum="9135411B73B8DD06" modified="2013-10-16" version="1">MNYEVQKKSIWFLLLLVSYFWIGLGFHTTCWASLRPSVHGWGKYIVMRRIFLIVAVLYFMRSITMYVTVLPVSSTTYFCSPKSNNTTPLELAKRVFQLLSGFGLSINGKHTYCGDYIYSGHTVILVLGYLIIAEYAEVDGRSSRACDDNPPLELNTFHSHLLIGYVHNSLTCTTTCSLGSPKSMPPPRTEVHYISTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRG8</accession>
    <name>T1GRG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRG8"/>
    <dbReference type="STRING" id="36166.T1GRG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006252-RA">
        <property type="protein sequence ID" value="MESCA006252-PA"/>
        <property type="gene ID" value="MESCA006252"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_1_1"/>
    <dbReference type="OMA" id="ECASKEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="14"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006252-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="24314" checksum="149D51058C240F20" modified="2025-10-08" version="2">MVSAGTVPFPDGKIVGGIPTTIEQHPYQVSLEKYGSHNCGASLYRTNIVVTAAHCLQGNINAKYLSVRVKSYINHPDYNGSTYSNDIAVILLEEHAVLNSSVRTIELATTTPRTGTPAVVTGWGTEEFGVRVYPNHLIEVELKIVDAKECASSKYRYGRRIESNMVCAVAKGKDACQGDSGGPLVADNKLVGVVSWGNGCAFDGYPGVYADVAVFHTWIEDTAESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSJ9</accession>
    <name>T1GSJ9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006659-RA">
        <property type="protein sequence ID" value="MESCA006659-PA"/>
        <property type="gene ID" value="MESCA006659"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2099649_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13659" checksum="5A36230EE6379C38" modified="2013-10-16" version="1">MYAISISSWKSVQLSDMINENKSRQILGYAEDINVIGRTILDIECIFLEIEKVASKWRKYQKHDLKQKREKSQPTRPEGQHGKVQHRGCEQFRLPGFRSPLQRWALHDQNTKSHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT78</accession>
    <name>T1GT78_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02200222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT78"/>
    <dbReference type="STRING" id="36166.T1GT78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006901-RA">
        <property type="protein sequence ID" value="MESCA006901-PA"/>
        <property type="gene ID" value="MESCA006901"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_2_1"/>
    <dbReference type="OMA" id="HFIATME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="40"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="226"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="404"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006901-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="54964" checksum="EB80F83E1EB5EA24" modified="2013-10-16" version="1">MSVYNSVEKVWYGSKETPAFHPDVSAGKVLEFVMKQHYPRIGLIFEPTGEQWTYEELHKTASTIAQNILAKGLTQDDVIGICASNTPYVTTVALAAFMTGIPISSLDPSFDKEGIQHIYGITKPTIMFCDGKILETVTESLRGINLETSIFVVDEKRGAGTIDEFLREIGGTFECPALKYGGDHSAIYLPSSGSTGLPKAVCISHKTLITTSFIEPTSTDTVFCFNYMLSVIEKYQVSVLASPPSQLVLIGQSSKLGTTNVSSVRYYLLAGSAIPFHVLQKIKEYFPNTIFSAGYGCSELGSCCCTGEVTAPNSNGHVRENYQIKIVDEDGNNLGVGETGEIVVKRPFPWLGYKGNPEASKDTYIDGQIFMGDLGYFDENGTLFVIDRKKDILKYKNFQYSPNEIEQVILELPDVVEVSVCGIPDLIATDLPAAAVVIREGSQLTESDVYNYVAKRMVEFKHLHGGVYFVDSVPKTVSGKNLRRVVKEKLIKLYDARKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTD4</accession>
    <name>T1GTD4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02097228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTD4"/>
    <dbReference type="STRING" id="36166.T1GTD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006964-RA">
        <property type="protein sequence ID" value="MESCA006964-PA"/>
        <property type="gene ID" value="MESCA006964"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_1_1"/>
    <dbReference type="OMA" id="ASEWVHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="9">
        <location>
            <begin position="43"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="9">
        <location>
            <begin position="370"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006964-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="569" mass="65714" checksum="4101280069EC9E51" modified="2013-10-16" version="1">MLTLFKKIPVHRFTRLTRPITQATTSFDIPEPDNKTKIAIEKYWADKLNHGSFKSQSDADKFYVLSMFPYPSGNLHMGHVRVYSISDTVARFYRMNGKNVFHPMGFDAFGLPAENAAIQRNVSPADWTNQNIAQMRTQLKQLGCSFDWNHEVSTCNADYYKWTQKLFLMLYKEGLAYQNEALVNWDPVDKTVLADEQVDANGCSWRSGAKVEKKLLKQWFIKTTKFAEKLLEGLSDPSLRDWKDIINLQRHWIGECNGYSFQLHFQGKPLSLRVWTESPEKLLDLNSFIVLKTTHPLKKALVFHDEVEFPEKCDVYLASPHYKESDRTLRASLNLPFQTNDLAESDEANRKVVLKIADLIGNGGYKVSSKLQDWLVSRQRYWGTPIPIIHCDSCGAVPVEEKDLPVRLPPADESLNRDIASLKCSCPKCGNENAKRETDTLDTFVDSSWYYLRYLDSQNPDKIYDEDIINKFMPVDLYIGGKEHAVLHLYYARFMNNFLYSKGLSPHPEPFQRLLVQGMVMGRSFRVKGSGKYLQEHKVEIIDAKKNKAICKETKEPVIMTWEKMSKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU78</accession>
    <name>T1GU78_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU78"/>
    <dbReference type="STRING" id="36166.T1GU78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007286-RA">
        <property type="protein sequence ID" value="MESCA007286-PA"/>
        <property type="gene ID" value="MESCA007286"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111647_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158:SF5">
        <property type="entry name" value="NAD KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007286-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="13375" checksum="1773879F2022D8E8" modified="2013-10-16" version="1">MLLRRKLRFLSEAAFNGCKKYGTVAAASTVSSSHEQPSAKSFKLQKALIVTKLSRYEYEESRNRELNSQELEKKLRNRGTDYDGMLYLHNLHKKFEMRVIKSFQDVGCDVKVINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU85</accession>
    <name>T1GU85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007294-RA">
        <property type="protein sequence ID" value="MESCA007294-PA"/>
        <property type="gene ID" value="MESCA007294"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673926_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007294-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8862" checksum="58AD7960ED4F5F60" modified="2013-10-16" version="1">MIEKQTSRQIKDIRTDNGLEYCNEPYVYNRTSSRILEYFKCPEEVYDGKKPNLKLKFLVLIKSICTSINTISPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUB2</accession>
    <name>T1GUB2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02097782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUB2"/>
    <dbReference type="STRING" id="36166.T1GUB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007322-RA">
        <property type="protein sequence ID" value="MESCA007322-PA"/>
        <property type="gene ID" value="MESCA007322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027611_0_1_1"/>
    <dbReference type="OMA" id="NEHLLIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="313" mass="34752" checksum="680CB29CCEFD5C80" modified="2013-10-16" version="1">MALVGFCLIMTFVYSKLVKFIFYPLLIVYAIIAVVIPITTKEVPVQILFEYLTSDLRKIFLLLIYFGLVVATICVISFQSNEGKASTRTRKLFHILTVLVFVPGTLFQCQFLYLATGVAFAAMTIFEIIRVADIPPFGNYLREAFTNFCDEKDCGILAVTPFYLLIGCSLPFWISPCPCLISDTVSKKDLLPLLSGVLTVGIGDTFASVIGSKFGRASGQVNIRNFYCDSKYIQNFFLFLGNNRSVEGTLAFIVSVSAVIAMLYFANLVQIDSVFKIVIISLAVLITSLVEAFTDQVDNLVLPLVFYTIVSCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVL7</accession>
    <name>T1GVL7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVL7"/>
    <dbReference type="STRING" id="36166.T1GVL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007828-RA">
        <property type="protein sequence ID" value="MESCA007828-PA"/>
        <property type="gene ID" value="MESCA007828"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157661_0_0_1"/>
    <dbReference type="OMA" id="MASACIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11960" checksum="298D0CD3A57AF5BA" modified="2013-10-16" version="1">MHRFYAFHSFTHFHRNGMASACIFLSCKVEEQPRKLEHVIRAAQICTNPEQGSNLQKEVYNEKAQDLVFNENVLLQTLGFDVAIDHPHTHVVKTCHLVKDDDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVM1</accession>
    <name>T1GVM1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02395045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVM1"/>
    <dbReference type="STRING" id="36166.T1GVM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007832-RA">
        <property type="protein sequence ID" value="MESCA007832-PA"/>
        <property type="gene ID" value="MESCA007832"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232546_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007832-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="225" mass="23995" checksum="2AADE9F02A856057" modified="2013-10-16" version="1">MAVATKDEDYLQWLQRKTRASLSIKYRIKRTEASKLCRQKKLRFARKEILRDRMSGQTPTTPTTTAMSSQSPISPNETSGLPLSPSGSSGVATGALSPGSASQTSTNAGPQTNPCCENGRPMIADPVTGQTVCSCQYDSARLALSASYSRIPASSVGVYGTPYPSNDQNPYPSIGMDSSAFYSPLSNPYTLKDSAGITDMSAWTSAGLQPTSGYYPYDSTLIPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVT0</accession>
    <name>T1GVT0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02004747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVT0"/>
    <dbReference type="STRING" id="36166.T1GVT0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007896-RA">
        <property type="protein sequence ID" value="MESCA007896-PA"/>
        <property type="gene ID" value="MESCA007896"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1148353_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019321">
        <property type="entry name" value="Nucleoporin_Nup88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10168">
        <property type="entry name" value="Nup88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="138"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007896-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="27584" checksum="06D297C0AB118807" modified="2013-10-16" version="1">METPQIMFSKPSHVEYIVCTKIDNSEKINPILGLAIHQHPSGLISFLSSGEVISLKLLFDSSSLNVMEKPNKEMYEPTSIETPIEGQFAAHIKNILQRKVSQPILSLNKSSQLCPQKAYEVLTQSIQVLKENYIKNHNEALKEILKRIDLQIKIKEQQEREIKNLEKERDIISQKAHLLAEKFEECNNKQSLISKLSYNLLKIGNVSLPNNIIAENAFTTKVNNIQATTKGFFEDLKVSRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW95</accession>
    <name>T1GW95_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008068-RA">
        <property type="protein sequence ID" value="MESCA008068-PA"/>
        <property type="gene ID" value="MESCA008068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760716_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8308" checksum="0DEFB1CABE015189" modified="2013-10-16" version="1">MTVQKWALSVTHRIQKVPLNCDIDTYWKQCVEMVKNLPLRYLVCNPRHDNMNGEDGEEYDRIIAENKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWC5</accession>
    <name>T1GWC5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097131">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02097132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02097133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWC5"/>
    <dbReference type="STRING" id="36166.T1GWC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008098-RA">
        <property type="protein sequence ID" value="MESCA008098-PA"/>
        <property type="gene ID" value="MESCA008098"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1196031_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008098-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="232" mass="25738" checksum="2D0EDD675585FCE4" modified="2013-10-16" version="1">MIKANFERSETEGKARLESRLDECMRETSALRRRLQEEQDNFRVEVEYQVPTSSQREYDSITVDSDEEDGCISEENNAEMDEGNGEGENEGYDEGYEQNQEDMDDVEEGDQSNDIDQSNVQAGDNEVETSSENADEIMIEADAQSSQVEAPQIQAISSGSSEVESSWRQAIPSTSAGLSRQQQATLLMLQKGCNETDGGSSEVSQAPQIQNHYVASQGQARPEISKNQTVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWE2</accession>
    <name>T1GWE2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWE2"/>
    <dbReference type="STRING" id="36166.T1GWE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008116-RA">
        <property type="protein sequence ID" value="MESCA008116-PA"/>
        <property type="gene ID" value="MESCA008116"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1706293_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="10"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008116-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17905" checksum="315C29025AE39923" modified="2013-10-16" version="1">MDEFLEIRLSQKSAPTYVYIYDHKGYGSLTEAVGGGDFFYGVCHADELQLLYPIGSVLYPTGVPSERDILMRDSFIDMWVNFATYGNPTPSGSKLPKWNETKGYPWNYATIGSTDNDKWFILKNEENYVRDRFNFWKSLKPHLHNKETINRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWH4</accession>
    <name>T1GWH4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008151-RA">
        <property type="protein sequence ID" value="MESCA008151-PA"/>
        <property type="gene ID" value="MESCA008151"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600343_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008151-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="456" mass="50133" checksum="BADDD43B06E081E6" modified="2013-10-16" version="1">MGLNKADSDTTVENQQQQQLPQNNISPLPINIITQNKNSKINKKILKEKNSHTNVIINNNNNSSSITNNNNNSKKIFDLNHKPNDSNLGVGGKTTMAPALTEPLRSANQLTSSSSPNAASASPRPHTATTNTNSSKKNGNTRLLNEFPMGPMSEPNTNITDTTSSMGTNNFENIIENIPITLVHLENDFEEAISAEICMKGGVVLNNSKDKFINNQQTEEPQNEQQQQRRQEPDLLLPSSPPASLASQHNIGRFWGFYERIGADGSIRKKSYSIHFEATCNSYDWRSRCLRCTAKSAIKSVIAAQKAAIKSERSSMQSILPGFKKNEKGELVPKRKYKKKIQVPSDFYSDDGGGSGSSTPYPANKGSKSSKKHHSRNNHSGEQGHSSSMYHQNLNSTIPTSSSAQRNKIRKISSSTSFLNSSSISNNGNSFGGNDSFDNLMDKIQFRNIPSIPQAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWK4</accession>
    <name>T1GWK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="MESCA008181-PA"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008181-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9549" checksum="13662EDA4A0F8AA1" modified="2013-10-16" version="1">FRISREWRQKIESWLQSNENDEKQNEEYKRRRLLANNSRRSLENETESDKSKLDPLPEEKPNSNNPDSKRVYKDWKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="synonym">Phora scalaris</name>
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            <taxon>Brachycera</taxon>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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        <property type="gene ID" value="MESCA008240"/>
    </dbReference>
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    </dbReference>
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            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008240-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="4893" checksum="E6D4621CD7A8636B" modified="2013-10-16" version="1">MRKEKKILRKKLMTITNLINRRGKGTTPRKHPSLVQVKQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU3</accession>
    <name>T1GWU3_MEGSC</name>
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        <recommendedName>
            <fullName evidence="1">DUF4704 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
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        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    <reference evidence="2" key="2">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="DUF4704" evidence="1">
        <location>
            <begin position="30"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15787"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17722" checksum="0D87A57EF18E19D3" modified="2013-10-16" version="1">SAICLKPETNNLHFKYPFINFYFIILRFPQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMSHDGCSDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLSLTKYLVTCLSANSDLLLKQMDEIEHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXB7</accession>
    <name>T1GXB7_MEGSC</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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    </dbReference>
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        <property type="gene ID" value="MESCA008462"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13462" checksum="F569826DBB94D127" modified="2013-10-16" version="1">MTPMDNGMRLIATAAAHNMSLLSLGSTPHKFITLNSLFPLSKIGEVNLEVEKAQFRGKLWLVSWMGKGKYLVNYLHLPTEGIFSSICITIICFLNDNYDAVFQIQEYSIHILKTSFTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXG5</accession>
    <name>T1GXG5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Brachycera</taxon>
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        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008516-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="18468" checksum="4159649898086FAF" modified="2013-10-16" version="1">MSNESRLQKKRTTHKKPQPLQLAFWIHLISSVFIVSLGFIDILPSSGMHMKSFQQFWKDSGSTFLCHGGISQYGIKNFTRNERINGEENVEILLPNLNIQNNEHYHRNKRILHLHNSSGGSAGSLGTHGSHSSENRHHLKNIIHGKLIEHEHIWVKLTPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXM5</accession>
    <name>T1GXM5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Megaselia</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA008578"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443344_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008578-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10202" checksum="26C4CE2DA220D320" modified="2013-10-16" version="1">MHFKIAVGTPAFLATLLLLPDFFFMIRRLPFYVLSENGSRNKSARTSLCVHFESTVGAKGAKRDIKRKGGKLPNFIDKREEDLSKKKSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY19</accession>
    <name>T1GY19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02027966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY19"/>
    <dbReference type="STRING" id="36166.T1GY19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008739-RA">
        <property type="protein sequence ID" value="MESCA008739-PA"/>
        <property type="gene ID" value="MESCA008739"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1671334_0_0_1"/>
    <dbReference type="OMA" id="HLDANIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033370">
        <property type="entry name" value="COG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008739-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="18000" checksum="93ED50FFCBA13DF9" modified="2013-10-16" version="1">MYETIGNEKFIESNQNAALQIYFDLKFIQTIFTKRDDRNILDRLQKISDTLKTFIDPFDFELFHTHLDANIKKCVVRMRAQLGILIPYPEKVDLLLSRNEVKLSSSSSSSSSPAGGEPNVICLSSSGSTSTWFPLLPIIVQEEKVLEEPKKDVQEIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY81</accession>
    <name>T1GY81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sodium-dependent nutrient amino acid transporter 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY81"/>
    <dbReference type="STRING" id="36166.T1GY81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008803-RA">
        <property type="protein sequence ID" value="MESCA008803-PA"/>
        <property type="gene ID" value="MESCA008803"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010359_0_0_1"/>
    <dbReference type="OMA" id="XTITTTI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="10"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008803-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="276" mass="31826" checksum="4786FF86201E4815" modified="2013-10-16" version="1">FQSPLPWSKCREEWGPYCVDSDEGNVDVVKNLTNMSLSYSSELYFVKEVLHERENINDGIGLPNWELVIGLFVSWAVIFLIIIRGVKSSGKASYFLAIFPYVVLLILLIRALTLEGSIDGIIYFIKPQWEEILNPKVWYSAVTQDTEFMIGLKVGVYWRICWSIITPAIMTVILVYTFIAYEPLTYKDIVYPDWAYSIGWTISIFGILQLPIWAIVAIYRQPEDSLKEKIFQAFSPKKDWGPTDPLLREKYLKEMSLETSRTEDGFLSFVKNNVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY94</accession>
    <name>T1GY94_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY94"/>
    <dbReference type="STRING" id="36166.T1GY94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008817-RA">
        <property type="protein sequence ID" value="MESCA008817-PA"/>
        <property type="gene ID" value="MESCA008817"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1024103_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008817-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="272" mass="30138" checksum="BFC126ABC237C98E" modified="2013-10-16" version="1">MKCNCPRVITPQKCGDVYVYDLIPSPPPSATISSCNSSQLFTLPNETVNDYDVPKPPTPILSTQLSYDFPKSSWCRSSFTQHNDMSTPPSSLVSRYSHQNSLPQLQEESYDVPRSSPNLKVLRQLQQSNITPSSSNSSLHLSDSLGSSISSSNRSSFMNASDYDVPRRNPIPVKKPYPLPNSSYIRSSCPSPSPSTSSTSGLATYDFPSPNPKLVLNKIEIAPKELPLSYNKRLKLWINCKMKLLPRLQELNENGQDIVSRFLSSGNISGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYV0</accession>
    <name>T1GYV0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYV0"/>
    <dbReference type="STRING" id="36166.T1GYV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009037-RA">
        <property type="protein sequence ID" value="MESCA009037-PA"/>
        <property type="gene ID" value="MESCA009037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504281_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9287" checksum="0AC1259F7DA77792" modified="2013-10-16" version="1">MARDFKTLLWDYIVFVLFILLSTLLPFWSKICGYLRTKGGGDAALATQTQNGGGKKADFVFATGSVSLLAIMLSIARGTLGIRSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZG3</accession>
    <name>T1GZG3_MEGSC</name>
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        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZG3"/>
    <dbReference type="STRING" id="36166.T1GZG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009263-RA">
        <property type="protein sequence ID" value="MESCA009263-PA"/>
        <property type="gene ID" value="MESCA009263"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1242805_0_0_1"/>
    <dbReference type="OMA" id="MENEHAI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF43">
        <property type="entry name" value="HECTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="48"/>
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        <location>
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            <end position="141"/>
        </location>
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            <begin position="153"/>
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        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009263-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="25221" checksum="E9EF291C96FFC382" modified="2013-10-16" version="1">NYFWVFCEGLHLHLALVVVFAKDSIATSWFLIIGWGLPLFYILFYALFSLYLTNGHPKCGMENEHAIWILSIPVSLSLICLFIILVNLLIVLYNRIQSQSKQSPMSLKRAIKTTFILVFNIVEKNICLLNVPLFGLQLFLIPFRPSENSAYEVYYELVSVFLVSLQGLFVSLLYCFANPDVICAFRVFLNRVMPTTFAPVPLTIHSGTGHIGTTTPSNAEVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZW6</accession>
    <name>T1GZW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZW6"/>
    <dbReference type="STRING" id="36166.T1GZW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009424-RA">
        <property type="protein sequence ID" value="MESCA009424-PA"/>
        <property type="gene ID" value="MESCA009424"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1911902_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006150">
        <property type="entry name" value="Cys_repeat_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028150">
        <property type="entry name" value="Lustrin_cystein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14625">
        <property type="entry name" value="Lustrin_cystein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00289">
        <property type="entry name" value="WR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="14660" checksum="4ADAD749912F11D2" modified="2013-10-16" version="1">GTCECRNFCDDISCSTGEECQLINVECVDKPCPKMPICIPKRESVCPEGSPLKQGDIEVTCGPHNEFEVCPTTHSCQLNPVTHRGVCCTKTRDVCFEGLDKTCVAKGDSEKNSTKWRFNPKVNKCVQVNINSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0M9</accession>
    <name>T1H0M9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0M9"/>
    <dbReference type="STRING" id="36166.T1H0M9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009710-RA">
        <property type="protein sequence ID" value="MESCA009710-PA"/>
        <property type="gene ID" value="MESCA009710"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005171_1_1_1"/>
    <dbReference type="OMA" id="HKYFATH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF8">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MyTH4" evidence="3">
        <location>
            <begin position="267"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="443"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="72667" checksum="4A0B760C8B25D91F" modified="2013-10-16" version="1">MEQRQSSWHDYGECVVLQCLQNPTRIDDIDLIGRSSVHVVESLLRLERAFERWLKLVEDDEIQEHLLPLHNYIIEQAKLSGTWIFGDLDEESLYSDSHSEHSMSGHEPDNEDSDHSDSGDYIKYNVIKLYDDIAQSIHHSDRTEDDDVEYLLEKENSKIYGSMDFGMPVLKSEETAYSKTLPTSKLMKPPTNLEETTNLTNITNTNTGKPESRTNNSKEENKAVLQKINNFATFYEYDLERFAKDNLNFHSKGLFRKKASVRDMIRWTSEAINKPMLALPHDKIEKKMAIDVFKLIQIYMGDRKARPGMTLNSVLMIFYKLQTTIKLGMKYICKYAVKRRRIPQKNPCYEVGNCWQFFAFQPALLNYINKHYDNTFITKFPDVKRWPVHVQISHYATVCRKRLDRLNARGGSFVKKCSTDDIEHSRNQIIRDSMFGNTLVVIMDLQKSMKLMDSDLPWIQVTLSEMILELGGRETEGIFRVSADADEVVRVKCMLDNFQIPAKSDSLDAHTPASVLKLWYRELEDPLIPEPLYNECISTTDADKARSIIDRLPYLNKRVFLYLLSFLQKFTHPSVVFNTKMDCSNLAMVWAPNCLRCPSNDPKVILENARKECMFLHLLLTQNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0S4</accession>
    <name>T1H0S4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0S4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009763-RA">
        <property type="protein sequence ID" value="MESCA009763-PA"/>
        <property type="gene ID" value="MESCA009763"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712018_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009763-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8394" checksum="BA5867B1F00DCA81" modified="2013-10-16" version="1">CWTSSFQKIHQYFASPQSLEYLTEESQKETVKSGSSIVVSTFQKREFGIHGESQMTDIFPFHLSLFSEYIPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H145</accession>
    <name>T1H145_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H145"/>
    <dbReference type="STRING" id="36166.T1H145"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009897-RA">
        <property type="protein sequence ID" value="MESCA009897-PA"/>
        <property type="gene ID" value="MESCA009897"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929965_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF89">
        <property type="entry name" value="POU DOMAIN PROTEIN 2, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="70"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009897-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14612" checksum="1B40F456ED7E3EF8" modified="2013-10-16" version="1">MLTSPLTNPEHMGRRRKKRTSIETTVRGALEKAFMINNKPTSEEIASLSDQLCMEREVVRVWFCNRRQKEKRVNPLEPDSPTGTPLSHPSLFLNIPPLSSSNNGFDSSSLGSISPNYSSPSGPISLKQQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H180</accession>
    <name>T1H180_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02375722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H180"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009934-RA">
        <property type="protein sequence ID" value="MESCA009934-PA"/>
        <property type="gene ID" value="MESCA009934"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654131_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009934-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="18888" checksum="D0A4D8776E0BEA5F" modified="2013-10-16" version="1">MQEIIWSEELEWGAGYYAESRRNYFNNSINNQLGITMPYPNCRETPNFPRAYLISETMKDKGLFKNIHYFSEFLKNMYELFLYLNIGNIQNQNSNVPIVSKESSKQIGNKTMDLDWVNKNRCMPCFTEHVIYFLSMFDDGFGVRATYILYHDILGCCKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1B1</accession>
    <name>T1H1B1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium-activated potassium channel BK alpha subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02087752">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02087753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1B1"/>
    <dbReference type="STRING" id="36166.T1H1B1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009968-RA">
        <property type="protein sequence ID" value="MESCA009968-PA"/>
        <property type="gene ID" value="MESCA009968"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443357_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcium-activated potassium channel BK alpha subunit" evidence="11">
        <location>
            <begin position="40"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009968-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10308" checksum="F2AD2727EEC668EF" modified="2013-10-16" version="1">MRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTVSIYVPIVIVHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1B5</accession>
    <name>T1H1B5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DNAJC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1B5"/>
    <dbReference type="STRING" id="36166.T1H1B5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009974-RA">
        <property type="protein sequence ID" value="MESCA009974-PA"/>
        <property type="gene ID" value="MESCA009974"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055735_0_0_1"/>
    <dbReference type="OMA" id="WFWRYTV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000036">
        <property type="entry name" value="dnaJ homolog subfamily C member 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044632">
        <property type="entry name" value="DNAJC25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44176">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44176:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577906" description="J domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009974-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="317" mass="37935" checksum="9B12D05EB5180B26" modified="2013-10-16" version="1">MLTFCLHLLVLLPTSSALLEGLYCGTENCYDVLGVTRESSRQEIVKAYRSLARIHHPDMHRTQEKKLEAEEMFKKIATAYEILRDEESRSDYDYMLDNPSAYYSHYYNYYRRRVSPKVDIRLVIIVTITLISVFQYYSGVSRYEEAITYFTTIQKYRNKAMEMAKDEINEKVNKKGKSKLSKSEQKSELEKIIRKVIEEKMDIQGAYAKPSIKNTLWVQLVLLPYTLGCYILWNIKWIWNFNILKKPYGDEEKLYLIRRYMSLGQNQFDAKEESEKQEYLEMELWIKENFLEWKQIQRKNRRSCWLIIPDTNPTEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1D0</accession>
    <name>T1H1D0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1D0"/>
    <dbReference type="STRING" id="36166.T1H1D0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009989-RA">
        <property type="protein sequence ID" value="MESCA009989-PA"/>
        <property type="gene ID" value="MESCA009989"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830172_0_0_1"/>
    <dbReference type="OMA" id="WNYENNI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF143">
        <property type="entry name" value="SERINE PROTEASE GD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009989-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15397" checksum="407CF01408ADBADD" modified="2013-10-16" version="1">FNSFKRPICLWNYENNIRGVINRKGVIAGWGSDENNLVSTEPKMLEVSIADPDWCLRSNDEIARHTTNRTFCAGDRNGKGPCHGDSGTGISIIENGRWTLRGLVSATLNKRKSDDCDFKEFTIYTDVAQFIPWILAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H224</accession>
    <name>T1H224_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136534">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H224"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010255-RA">
        <property type="protein sequence ID" value="MESCA010255-PA"/>
        <property type="gene ID" value="MESCA010255"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545209_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588685" evidence="1">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010255-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9115" checksum="C521DBC52CC8881B" modified="2013-10-16" version="1">MKLIVTIALLVVAGAALIDAKGPPEFMAQFKECMESNGLDPKQGKEIFKKLHSGQDLEGDEKKNFGCANKCMMEKQGVWKDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2P6</accession>
    <name>T1H2P6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2P6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010499-RA">
        <property type="protein sequence ID" value="MESCA010499-PA"/>
        <property type="gene ID" value="MESCA010499"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253085_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010499-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12472" checksum="66147AAFB8B77486" modified="2013-10-16" version="1">MSAKFTTFSTFNIKIYRKFTSKSNQIEPLFTEIVYSKLFYSQLDNFLDCSITIKCKYKQFQATCWKECKLSRTTTTNLKSRIFEYSNSKCLSQRKKKQFSKIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2T1</accession>
    <name>T1H2T1_MEGSC</name>
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        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02052662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2T1"/>
    <dbReference type="STRING" id="36166.T1H2T1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010535-RA">
        <property type="protein sequence ID" value="MESCA010535-PA"/>
        <property type="gene ID" value="MESCA010535"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898626_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010535-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15183" checksum="3DFAE5802BFE0930" modified="2013-10-16" version="1">MELVGSSKRTVCGISFQAVFAGGIMLVAGWGALINDRVILQVVYGLHALILIPHWWIMDESPRWLWMQGRKSEAIDIVAKAGNIENFGTNFIIYYKNYLKNEKLLRISFAKILDELNSKNLIISLAFTIKGLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2U3</accession>
    <name>T1H2U3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02372970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2U3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010548-RA">
        <property type="protein sequence ID" value="MESCA010548-PA"/>
        <property type="gene ID" value="MESCA010548"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815342_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010548-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7565" checksum="A1397678BEB29F82" modified="2013-10-16" version="1">MTGVRRKLSCSALLLGLPKNDIQKTPEQTILNDTLMCPNGRSTLNRVEMAYAREAYNYNNLELHGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X0</accession>
    <name>T1H2X0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2X0"/>
    <dbReference type="STRING" id="36166.T1H2X0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010576-RA">
        <property type="protein sequence ID" value="MESCA010576-PA"/>
        <property type="gene ID" value="MESCA010576"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_877965_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010576-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="317" mass="35434" checksum="AC7526B2B8DEE7CE" modified="2013-10-16" version="1">MYTSPYASADNLYMTPSVHSTNFYPVSENLFHQYRLQAGVGGYYTDYHHTSTSASYLTNGFVSYEGYGIQVTSNSNKEEKWQDSNKYYSGYSSGYGSPSSTPSLPLKTPKSSPQQIMEVNEGSSPTNQQIVSNSVQTPANGTIIVAPKIENSPPEPFERQTVLMWGAPNSSEQSVTPGTNTSTRSPSSTPTSNNELYVETKLDEQQSNHLKWNGTMVKNTPTSTTVDIYQMPHQPHSDNSIYPQHIHNDDIHSAENSHLHQPHLQSSSLSGCEVWSPAPYSSQYQYFSYHHHATNNQHANTQWCHKLTKGFSFLLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Z0</accession>
    <name>T1H2Z0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Z0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010597-RA">
        <property type="protein sequence ID" value="MESCA010597-PA"/>
        <property type="gene ID" value="MESCA010597"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963400_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6875" checksum="6C652EC4AE2032F1" modified="2013-10-16" version="1">MMIDQLSKCSPKHRRDQEGRGRQRTRDYGGQDKLNATQTVFCREQQGLCCLVFTHCSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3F7</accession>
    <name>T1H3F7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3F7"/>
    <dbReference type="STRING" id="36166.T1H3F7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010783-RA">
        <property type="protein sequence ID" value="MESCA010783-PA"/>
        <property type="gene ID" value="MESCA010783"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691157_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012768313" description="Immunoglobulin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="2">
        <location>
            <begin position="22"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010783-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="16590" checksum="FF3273FE1EA474C4" modified="2013-10-16" version="1">ILNRICLFLAHLINAQKIEVHPENVVVKEGGSTTITCRSPGQQITFCLFRINGSSALNVRDGFNQNGISYVGAGLASGECGINIERVQSINNGQVQCTLGVEGGPNGVIEGYGQIIVAKAAEPQIQVEYGQRNGLFSTEVPLRASCIVEHARPKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Y2</accession>
    <name>T1H3Y2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">60S ribosomal protein L18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Y2"/>
    <dbReference type="STRING" id="36166.T1H3Y2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010979-RA">
        <property type="protein sequence ID" value="MESCA010979-PA"/>
        <property type="gene ID" value="MESCA010979"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1490659_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="28"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010979-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20876" checksum="0ECE3C290DC9D9BD" modified="2013-10-16" version="1">MVLHVVPFLSYQQKIQQDYVETLVYEQDQPMSLQRVAIFLKKGGQKDVTAFVVRTVTDDIRLLQYRKSSSTYHQYCKVLTFDQLDLKSPTGKKTLLIQGKHTACEAHFSLPHSPICPLQGAQIRMYSKCIYLKLTSQKCSAKKTKRTPNLVSKGSFQRSTKCLEYKKDTNLIDIDPERIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H481</accession>
    <name>T1H481_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein 194A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385713">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H481"/>
    <dbReference type="STRING" id="36166.T1H481"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011084-RA">
        <property type="protein sequence ID" value="MESCA011084-PA"/>
        <property type="gene ID" value="MESCA011084"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025225_0_1_1"/>
    <dbReference type="OMA" id="MTETRIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598:SF1">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011084-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="26356" checksum="700C6824A873AA42" modified="2013-10-16" version="1">MLYIQNTLFYYITGILIGIFASFLVLLYFLGKLLPKRKMVYGALIGGYAVGIYLVQFLWENLQLIMISYQTYVFWYIFVTGIISFIVCYRFGPPKNQRSKNLIKWGLQLAAVMMIFFSSHFEEATTAIILLTTALHYFPQSFTKNIRSWYKRKFPPKRRLLTNEEYFEEGVRETAIALNKLRDFCSSPDCKQWKIVQKLKDPMRFASFIEGESHLTVQKCCLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4U9</accession>
    <name>T1H4U9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02042754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4U9"/>
    <dbReference type="STRING" id="36166.T1H4U9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011317-RA">
        <property type="protein sequence ID" value="MESCA011317-PA"/>
        <property type="gene ID" value="MESCA011317"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057768_0_0_1"/>
    <dbReference type="OMA" id="IMAHERE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="303" mass="34468" checksum="7878DB85DAE2A174" modified="2013-10-16" version="1">IVEDLWTPFKDLQSIVERCTTQPSKITIQELDTVLRRHKQNFTSLLRNPPKNESSRNQLKAWLTEGKNIPGHGHILLSKDLADETIIISDMYDLEEFMALEMLCTAQQQMPQHPGLPRGLVAILLYYDGRRALACTLRDLFGAKLGVTWHIDSPKKITYVITSFVENLVENSNILERIIDLLEELDISKELAILTKNRALGPPKHHRHVLDLFEDIRLALATALFNWSAQSGLPKAVTTKLINYFSKYKPTEAWGGMDKVNTTLLMSLLYAFDTSVLQRYEDGDRRVQSLPIISEAGYAQHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H569</accession>
    <name>T1H569_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H569"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011444-RA">
        <property type="protein sequence ID" value="MESCA011444-PA"/>
        <property type="gene ID" value="MESCA011444"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725072_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011444-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="7973" checksum="38E2435F5434F5CE" modified="2013-10-16" version="1">MWLKDTNTLSSSKHRSTNSPNYKEYKGANYETSKVPDIHNFTSMSIAIALSLGISKSLASRSSRTTHLNLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H595</accession>
    <name>T1H595_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02380936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H595"/>
    <dbReference type="STRING" id="36166.T1H595"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011470-RA">
        <property type="protein sequence ID" value="MESCA011470-PA"/>
        <property type="gene ID" value="MESCA011470"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194033_0_0_1"/>
    <dbReference type="OMA" id="MQAEVQH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051234">
        <property type="entry name" value="TAO_STE20_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011470-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8527" checksum="D2DD22A687828396" modified="2013-10-16" version="1">MLQKQSLRLDESQEQECQQLSDTLQYELDILRAYQTKNKNQAEAQRDRERKELQERVSIRRSLLESKVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H621</accession>
    <name>T1H621_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H621"/>
    <dbReference type="STRING" id="36166.T1H621"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012154-RA">
        <property type="protein sequence ID" value="MESCA012154-PA"/>
        <property type="gene ID" value="MESCA012154"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077196_3_1_1"/>
    <dbReference type="OMA" id="WRWYVDG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000001">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 21 kDa subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012154-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="18087" checksum="F538D8176856EA63" modified="2013-10-16" version="1">SQRKSTNAEAPIVETNLVLATPEELKQKNKLAGTITVPKQVNIAPITGHPEEHVKTRRVCISRPAKNSMQSGTNNLDYWKIDFDNRERWENPLMGWASTGDPLSNMQVEFNSKEEAIAHCEKNGYKWFLEQEEQAKPARVKNYGSNFSWNKRTRVSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H684</accession>
    <name>T1H684_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H684"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012217-RA">
        <property type="protein sequence ID" value="MESCA012217-PA"/>
        <property type="gene ID" value="MESCA012217"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203576_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008496">
        <property type="entry name" value="TMEM222/RTE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05608">
        <property type="entry name" value="RTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012217-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12328" checksum="F325B597CCDA9865" modified="2025-10-08" version="2">MPIPELHGKNYPFLIINYTILIFNIFRFFPFIGHMGIALSNGVIRDFAGSYFVSEDNFGFGKPPPHIFLDKKCVKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6D2</accession>
    <name>T1H6D2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ras-related GTP-binding protein A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6D2"/>
    <dbReference type="STRING" id="36166.T1H6D2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012265-RA">
        <property type="protein sequence ID" value="MESCA012265-PA"/>
        <property type="gene ID" value="MESCA012265"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044099_1_0_1"/>
    <dbReference type="OMA" id="LIPNMQD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000002">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000276">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012265-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34670" checksum="CBBB52AA67648ED7" modified="2013-10-16" version="1">VLLMGKSGSGKTSMRSIIFANYIARDTSRLGATIDVEHSHVRFLGNLVLNLWDCGGQESFMQQYFTSQRNNIFQNVEVLIYVFDVESREIDRDVHHYQSCLEALLCNSPNAKIFCLIHKMDLVPEEQRESIFQGRETDLKLLSKPGEVTCFRTSIWDETLYRAWSSIVTMLIPNVAALENSLSYFANVIEADEVLLFEKATFLVISHCQGERYSDTHRFEKVSNIIKQFKLSCSKLGAKFQSMEVRNSAFAAFIDTFTKNTYVMVVMSDPNLPSEAALVNIRNARKYFEQLENPNNQTHQYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H749</accession>
    <name>T1H749_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">deoxyribose-phosphate aldolase</fullName>
            <ecNumber evidence="4">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphodeoxyriboaldolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIM1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DeoC/FbaB aldolase family. DeoC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="4"/>
    <dbReference type="STRING" id="36166.T1H749"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012531-RA">
        <property type="protein sequence ID" value="MESCA012531-PA"/>
        <property type="gene ID" value="MESCA012531"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968296_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046386">
        <property type="term" value="P:deoxyribose phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF3">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012531-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="32300" checksum="DDAE9726208F7BBC" modified="2013-10-16" version="1">KMYELYDPCTVMFFFRNKHIMIDLGTGNNNKINWPMEDKQEMIDIVETVYRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHTAAICVYPSRVADAFEAFKQLKKESVIPIAAVATGFPTGQYSLKSRLEEINFAIESGAKEIDIVINRTLALQEKWEEVYEEIVQMRKACGDKARLKAILAIGELGTMENVYKASMVAMMAGSDFIKTSTGKESVNATIPVGLVMINAIQDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Z9</accession>
    <name>T1G9Z9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02026689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02026690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Z9"/>
    <dbReference type="STRING" id="36166.T1G9Z9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000041-RA">
        <property type="protein sequence ID" value="MESCA000041-PA"/>
        <property type="gene ID" value="MESCA000041"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_1_1_1"/>
    <dbReference type="OMA" id="MWLDFVL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF937">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000041-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="19354" checksum="7BC00FBFDB11C9D3" modified="2013-10-16" version="1">ETKCSNQCTCIIDQIFTGCLQSDVVCQSCRGVSTTIDPFWDISLDLGVVNQTSTPSLLDCLERFTRPEHLGSSAKIKCSTCKTYEESTKQFSIRTLPIVASFHLKRFEHSTSLQVDKKISTYISFPAQLDMTPFMSKNHENAYVRHHKDVWVKCDDHILSIASLKQVLDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAM4</accession>
    <name>T1GAM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000283-RA">
        <property type="protein sequence ID" value="MESCA000283-PA"/>
        <property type="gene ID" value="MESCA000283"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654143_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000283-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="19299" checksum="4B1163E83EEA8B0B" modified="2013-10-16" version="1">MLIQVTMENNTMPRIRKRDPLCFKKFSYQKHISPAPQNLYSDMEPPKWYVHKPDRPCYLSHAMHQTSLTFIVYTPFVFAGYFKRIKFDEKASLKCFGHKIKERCRYKLRPPPSSIVDVITFVECIDHSEMRVKFGWGYDEYDRAEKKAYKKYQRLKEQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAY5</accession>
    <name>T1GAY5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Minor histocompatibility antigen H13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02136629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAY5"/>
    <dbReference type="STRING" id="36166.T1GAY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000402-RA">
        <property type="protein sequence ID" value="MESCA000402-PA"/>
        <property type="gene ID" value="MESCA000402"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092505_1_1_1"/>
    <dbReference type="OMA" id="EASEMWN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000402-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17347" checksum="D334948BA14BCD31" modified="2013-10-16" version="1">MVTVAKSFEAPIKLVFPQDLLSNGLNASNFAMLGLGDIVVPGIFIALLLRFDNSLKRKVKLYFYSTFAAYFLGLMATIFVMHVFKHAQPALLYLVPACLLTPILIALLKGDLKRLFAYEDNPEEKAKKEKAEKESNTNKDKESSSSKKKDSKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBN9</accession>
    <name>T1GBN9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Mediator of RNA polymerase II transcription subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Mediator complex subunit 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED11</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBN9"/>
    <dbReference type="STRING" id="36166.T1GBN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000675-RA">
        <property type="protein sequence ID" value="MESCA000675-PA"/>
        <property type="gene ID" value="MESCA000675"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123010_1_0_1"/>
    <dbReference type="OMA" id="WHRIQHV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019404">
        <property type="entry name" value="Mediator_Med11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22890">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10280">
        <property type="entry name" value="Med11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000675-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13768" checksum="11119F33542EEB69" modified="2013-10-16" version="1">MNTNEIFKIPGNALLELSKEKSSQKNAENHTQAFLKNLAQIEKQLSEQINYLSQVSTGHPHEGSSYASAKVLQMAWHRNSQTRERLMELEELRTKYSQVNAQRQQQQQQQMQQQQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBS5</accession>
    <name>T1GBS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02034320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBS5"/>
    <dbReference type="STRING" id="36166.T1GBS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000713-RA">
        <property type="protein sequence ID" value="MESCA000713-PA"/>
        <property type="gene ID" value="MESCA000713"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_432533_0_0_1"/>
    <dbReference type="OMA" id="CSVAYNG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000024">
        <property type="entry name" value="Low-density lipoprotein RecePtor related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000012">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000078">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000030">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000062">
        <property type="entry name" value="Terribly reduced optic lobes, isoform AI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF14">
        <property type="entry name" value="MEGALIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="17">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="386"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="405"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="529"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="556"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="568"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="593"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000713-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="633" mass="70973" checksum="68AA4100F41D8709" modified="2013-10-16" version="1">MDGTMRETIISTKIYWPNGLTLDITTQRVYFADSKLDFIDFCYYNGTGRQQVLAGSHYLLHPHSLSLFEDTLYWTDRQLNRVLSANKFQGKNQTVVSHLISQPLSIHVHHPSLQPLHNNPCEGSHCQHLCLLSPSSKTGFTCKCKPGYKLLDDGHCVEEENPFLMVVKGSQIVDVPLNGGDARAGSLTPKAIVDKDLSQPSGLAIDYDERKLYWTDAVREKIERANLDGSNRENLISATIYPFAISVFRNYIYWTDLQLRGVYRAEKHTGANMIEMVKRLEDSPRDIRVYSKDRQKCSVNPCHINNGGCAQSCHPAPNGKAECKCDDNSKLVNEGRTCAPRNHTCESSKFYCQNGKCISRMWACDGDDDCGDNSDEEPNYCSYHSCSPNEFRCNNGRCIFKSWKCDHENDCKDGSDEVGCTYPPCVDGEFTCTNGRCIPMAQVCNGVNDYDCRDNSDELPENCPQCNPESDFKCANNRCVPKQWTCDFSDDCGDGSDEVETLCKGSYRECSESEFHCGNGKCISSRWRCDHEDDCGDNTDEQNCEGYQCKNGTFQCASGHCIASYFRCDGDKDCRDMSDEVGCPPRYPGGRYCPESRFQCNNNLCVSPSDLCDGTDDCGDGSDENPEVCSNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC12</accession>
    <name>T1GC12_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC12"/>
    <dbReference type="STRING" id="36166.T1GC12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000807-RA">
        <property type="protein sequence ID" value="MESCA000807-PA"/>
        <property type="gene ID" value="MESCA000807"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2549519_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000807-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9482" checksum="2E64282CFF050BEA" modified="2013-10-16" version="1">MTIYNINVKPLLPFRSYLHLPPEIVQATLKDPARPETRRPRRITTPRSDSNKTSEDSSNYRCEPGDVDKKRNVGSGDSNLEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC83</accession>
    <name>T1GC83_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">inositol-phosphate phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Myo-inositol monophosphatase A3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02032442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC83"/>
    <dbReference type="STRING" id="36166.T1GC83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000887-RA">
        <property type="protein sequence ID" value="MESCA000887-PA"/>
        <property type="gene ID" value="MESCA000887"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034742_1_1_1"/>
    <dbReference type="OMA" id="FSNTRED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008254">
        <property type="term" value="F:3'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052834">
        <property type="term" value="F:inositol monophosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000012">
        <property type="entry name" value="Blast:Putative inositol monophosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF4">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000887-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="19301" checksum="57E5E638264EC4E9" modified="2013-10-16" version="1">MMNVRINRGAVLIIGVFVFVALIVLLYSNDNEANELYTNLRHSRQVNLRKLLIASIKASKAGGIEVVQISKSHDLQQKSKGKTKEGVDDPLTAADSRSHCVMKSGLHRLFPKVEIISEEDKTHGGCQDITSYFDLDPTVIHENVLIPDEMVNVDDVTVWIDPLDATKEYTGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDN7</accession>
    <name>T1GDN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDN7"/>
    <dbReference type="STRING" id="36166.T1GDN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001426-RA">
        <property type="protein sequence ID" value="MESCA001426-PA"/>
        <property type="gene ID" value="MESCA001426"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014864_0_0_1"/>
    <dbReference type="OMA" id="EEVWIIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF174">
        <property type="entry name" value="GH06923P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="262"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="295"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001426-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="67879" checksum="5B3DE654003519DC" modified="2013-10-16" version="1">MMEEVSLTELETIQYSNLFSYCDVEKTGKVPMSKAAELFETSNLSDEVIRQITSLSGIPETALHISRSTAQQSGVPIRFELLASSISLPPPKFSWKEPLSPTPPQVSRKNSAASSIAASTYSNASSGTIVDVNGLCAQSVSRDSTLRRGVYPRSPDLIELAAQLDSAVSDIQSTDSEVEHNETITTRRRRRCGSPEAWSTNSDSPTPTNSVAERPWAKESLWQGLLGDEHRQLLGTEEESSDRHSSDDEGEEDAIFQMTAEQKDYYYKQFIALQPNPKGLLSGHLARVFFEKSKIPTEELRIIWHLSDVTRDGSLSLEEFTAAMHLIVLRRHNIPIPSVLPLCLHPSVILGEVREADLLHLNDDVDLDKTLVDTTPKGNDGASIVVGGVVAGTISSTKITPPPIPPPIKNRSVSNSPPPSLPPVEPKSPPPRLKEVPKELNNQNREWTKFPESPTSTVSSPGPKPVNFDMKITSQAIVSDPQILHPVPLRVTPVGAETTQNQDSEVSHSPRPPQHAAPPVPAPRESIPNSNHSSAIQRPQAKKLPQKSIGGIPPPPQREPSIGGTNGEVPDSMISSQIQPPNMIPPLPPPRPHRHTRSSSLDLNKLKLSTGSGGGGNGSKPTQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE09</accession>
    <name>T1GE09_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02167168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE09"/>
    <dbReference type="STRING" id="36166.T1GE09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001566-RA">
        <property type="protein sequence ID" value="MESCA001566-PA"/>
        <property type="gene ID" value="MESCA001566"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1919451_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001566-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="14187" checksum="9F503BD6140C3F6F" modified="2013-10-16" version="1">MAAFGGNSMISPPSSNSNGDEPFGQLPPLPPPLRSTEILQPLTVFPVSNLSEDSYDYVFGGRRETPPVTSSIKLTSPPIRIRTDEHTATLIGEPTSSTSHLQHTSYSHCKVVHSSSRFPPRTPPRTTSASAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEC3</accession>
    <name>T1GEC3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEC3"/>
    <dbReference type="STRING" id="36166.T1GEC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001683-RA">
        <property type="protein sequence ID" value="MESCA001683-PA"/>
        <property type="gene ID" value="MESCA001683"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_299627_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="695"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="802"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="900"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="947"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="948"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="982"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001683-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1001" mass="115237" checksum="C798B2577333C7D6" modified="2013-10-16" version="1">MHSHNSNITYFKANILNYEISFRFIQEMATNWTKLKRLDIFISHSIDFTRRQLKYDLDQTPAFNNLETFEFHSDLVNLSMYFFKYFRMPNIKNLDIDLQFYMHAERNGTKERSNKIIPLIVKNSPLIESLSIYYGIHITPKTISNIVKNLPNLKEISLDKCANLPLLTIQNILKYSKSVQVINMKYFKDIKILRENVKIQNFIRKFCASLTPPLRPVTDNCNIINKLQRDGVDVILDPEYFGIQPYCSDSESDLDLDSDDGDDDDEDDDGQDGNGDELEEYSLTLIQKLKMSQSLFSRLNIFVTLTTLKQNRQWTENNQQPPPPLMSVHVEKPDEIGESEEPPKDSAELVLPKALEDVFALKDIRQAQFPGVEEQIQAEADKTSGNFADDDRDSDEENNANGENGDLDKKLKQSKAEKTKEKRNKQNRKLRQQVAKNEPPREEEDEEASKDPKSKTNSEETDDLDDIDVEYVPERITIADLAPMHRQFYRVFEIFKLENKPKASDKDKVVESEDKKKKSDDKMADDEDDDVEEEPKKEDKEKLSKRKLKKLTRLSVAELKQLVTRPDVVEMHDVTARDPKLLVQLKAHRNTVQVPRHWCFKRKYLQGKRGIEKPPFDLPAFIKKTGIMEMREALQEKDDAKTLKSKMRERVRPKMGKIDIDYQKLHDAFFKWQTKPRMTIHGDLYYEGKEYETRLKEKKPGDLSEELRIALGMPVGPNAHKIPPPWLIAQQRYGPPPSYPNLKIPGLNAPIPEGTSLVTMQVDGVNLLSMRMENHCMEMYLEPVSSTWIGIDEGDIERGQWGELESESEESSEEESESEGEEIPNIPDQSGLVTPAEGLVTPSGLTSVPAGIETPENIELRKKRIENEMEENETPVLYQVIPEKRTDRIGASMMGSTHVYDISSVNKPPPPMRPPGASGGDREGMVELALDPSELDMDNDAMAQRYEQQMREQQSHLQKEDLSDMLAEHVARQKSKRKRQQATESSTSSKTGNKKYKEFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GED2</accession>
    <name>T1GED2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Condensin complex subunit 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02096767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096768">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GED2"/>
    <dbReference type="STRING" id="36166.T1GED2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001693-RA">
        <property type="protein sequence ID" value="MESCA001693-PA"/>
        <property type="gene ID" value="MESCA001693"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_277815_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23731">
        <property type="entry name" value="ARM_ECM29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="6">
        <location>
            <begin position="486"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="5">
        <location>
            <begin position="838"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001693-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1141" mass="129076" checksum="228018355B09FB6C" modified="2013-10-16" version="1">MSSEEATELKNNVKFASFHLLSFILRDSSQKTKLREQAALCLGSLANGDSDFYSTRVLNKFLNIIKLQKDAVINIAIGNAFAMAINSESSSESSKEKYLKEYLKILIKLADDPNPHSRESVSVWLLSIVKNCSNYACFKDVRIYIQIAFTSLLADNSEFVQDVSSRGLGLLFSMADKKTQDNLANDLLNQMIGGKRSVNLVDMDTTLFPEGMLGKTPTGGNITTYKELCSLASDLNKPDLVYQFMQLANNNATWTSKLGAAFGLQSLSIEDKSKLEPYMRTIVPRCLLLGHYERNFRNITFNEWRIRIACCLAVRDLLKRKDGLKLRVDTENTISKDVPEPELKDLWFKLFRVMDDVHEGTRLAAKGTTELLGKLCSQTCSSDNRRRTQAVVKTIIPFILETGITHIVPEIRQISIKTLTEIINTSESLIVPQLKNIVPALLIAAGELESSQLSYLSTRLEADSDAQETVDTLRAESIKNHKISIHLGSSMQFLVGRYLKVCLTGINDRNPTIRKYFAKSIGNLIGIAKDASIKNLFTKFQEIYFENPHKGIPAAIFEINNKHPELLKDYFENILPLIFFAIHEEVSEETKSTVEMWKQLWDDISPGDAGISSNLNQILELITKNIVSEKWDVRVQSGAAINTIFMKLNHTIDKELRLTLINYILNAIHGRLFKSKDVFLKALVSASFGIRNTDEISKTIMEAVMNECKRKDIQYKTKAIEACGKIAEELEVDAWEEFFGLVSSILDSTDFQSLNASEETVCISLGRCWPKKSVGTQNKFQFQFLQKCEQCIVNSTRQVQIACLIALSKFTERIHVFDKNYDPTLNEYNLEKTDKINLMRNISTKILEIVSNSVSINNTSIKQECLTVLKSWLNRVLEEHNKSLIKVGDFKVELTNILKNLQKDNSPSLVYNVEQFENNLITNGFWINKYFKSSCKNSEEPVDVQNATYLALGQLARSCPAVFNQDLKIVLSYFNNLKIANPELHGPIKDTLISIAPSFACKTSATNFVLSPQLNLLLAMLRDNVESDNLIVLNATREFLTICFPEYFPESRYLLLIIAGKRNSIYETILFSLYGSTTKDHINYSSLTSKDSTENDSESKICLPSFNDMVNYVSEVIQKYSYSKDEKKSKVIPFNSTTFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEJ5</accession>
    <name>T1GEJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEJ5"/>
    <dbReference type="STRING" id="36166.T1GEJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001760-RA">
        <property type="protein sequence ID" value="MESCA001760-PA"/>
        <property type="gene ID" value="MESCA001760"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008987_0_0_1"/>
    <dbReference type="OMA" id="CIENAPG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000082">
        <property type="entry name" value="Plasma kallikrein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040479">
        <property type="entry name" value="CLIP_SPH_mas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF140">
        <property type="entry name" value="BCDNA.GH08420-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18398">
        <property type="entry name" value="CLIP_SPH_mas"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588217" description="Peptidase S1 domain-containing protein" evidence="2">
        <location>
            <begin position="31"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="662"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="527"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001760-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="910" mass="98712" checksum="B0039468BE384670" modified="2013-10-16" version="1">MGKTNNYNFIVSIVLAILVLAAHAPTTVNAQDDSLAGSFLSGLLDTITSTADSKDCPGVCVHTLATLICYEVLDDIPCPSANMKCCIESAPASKSPAVTHEPPPPPTTTTTTTTTTTTPKPKPTTKRTTQPPKKTTARPPVKTSAVAETSEKTSDGENACAGVCVADRIAEYCEAYLKTPGLCKSGTKCCVSIDEYEGKNTNDLYIPAKHMSPNHTITTTTSKPKKSTTQEPAEQKTNPNPKPSKNSLKKVVTTEPPLADDEEEEEETSNGQGKRIVKETVSMAFCPSDESCCITRTSDASKKPPTPGKPEKKTSPPPPKNKIPIVTSPRPPPPSQTSSISGDLLSQIWNIAETTLTNNLSPAQAQSPPPPQKCPGFCLLNIMAAFCERPSVVVPYPTSCKNGNICCDNTRAPPRRPPPPPPTTPAPVTPPPDPREECPGSCIVSLLSFTCFRNAEMTEVFKCPKAGQMCCAPKTPPMYPPQPQQPYPPPPPQQPFTAIPPTQKPQYPPPPPQSQYYPNQGAPVYGQQYPQQYAPQPYPPTTTTTTTTTTTTTTPRPAVYSKYVCGVKGNRRTTRSAETLDYETYKYEKYGRASRQLRIMENSTVSKSTERLILGTEIVPIQINNDKLGDLVQRFDETDDEILRRRFARMTQNNTLIRRARVVGGEDGENGEWCWQVALINSLNQYLCGAALIGTQWVLTAAHCVTNIVRSGDAIYKVGKSRSSDLKSCTTYIHHNHNSQTLDNDIALLKLHGQAELREGVCLVCLPARGVNHAAGKRCTVTGYGYMGESGPIPLRVREADIPIVSDTECIRKVNAVTEKIFILPASSFCAGGEEGNDACQGDGGGPLVCQDDGFYELAGLVSWGFGCGRVDVPGVYVKVSSFIGWINQIISVFVKLTNEMYIIFKIFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEN2</accession>
    <name>T1GEN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine protease gd N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEN2"/>
    <dbReference type="STRING" id="36166.T1GEN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001800-RA">
        <property type="protein sequence ID" value="MESCA001800-PA"/>
        <property type="gene ID" value="MESCA001800"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623132_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031986">
        <property type="entry name" value="GD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16030">
        <property type="entry name" value="GD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577317" description="Serine protease gd N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Serine protease gd N-terminal" evidence="2">
        <location>
            <begin position="25"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001800-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="19053" checksum="87484F3F0532FF19" modified="2013-10-16" version="1">MKNKLLILLLFIADAYLKELKIPISPCPDIFHYAYDGTEVYGIFNFEGAELNKIYNLKLHMVYEDTVYIKGKGKLALIEDQESALLNITRNKPVSYKIKFPIQTEIPEVTEIYMNNILLCFNEDSIEQSNTQIIYHNIFTTFGLTKYKSFTDFADPDFLRSQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF35</accession>
    <name>T1GF35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001964-RA">
        <property type="protein sequence ID" value="MESCA001964-PA"/>
        <property type="gene ID" value="MESCA001964"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_807229_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001964-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="344" mass="38587" checksum="1876B876A3683DFE" modified="2013-10-16" version="1">MENSEEKNEVHTTVRPENDGDKEISESGTQTGSHGTEIQNSEEQIEEHTTVQPENDQDKTIVENVIPMEHQEIGSLDSDDSTTIIPENKLHISSEDHKLESDTNLGSKDSNQIVSEYNENITDDKHTTEYPTNVEGVTTSHDSKVDTGIQHEELSTERPVKTDNNLADDILLTTNQLHSEKPTENFTTVHPDVDEQNKLSEPDDNKMDSEKQFEELTTSNPINEVRKEIIPNESANNKLDNQFEDFTTVHPFETNEIASSEEVTTVHAPEKDNELVENNAIPDSSDLNKEENQEQESTTLSSTFDNKSDSPNDIIVDGNVGVDSSDNIKLDSKKDDVDLLQFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF71</accession>
    <name>T1GF71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002002-RA">
        <property type="protein sequence ID" value="MESCA002002-PA"/>
        <property type="gene ID" value="MESCA002002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309196_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="12020" checksum="228BE8418CB4A358" modified="2013-10-16" version="1">MTLFIIFEKLHGNDKELGIFIIRFAYIFVSDLLIYSSHQEFNTQFQMLEFMGNRNKTRKFYANIGKRSYERIEVCYQVETKAIPRHYDFDNNMKVDACLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF83</accession>
    <name>T1GF83_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="7">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02184400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF83"/>
    <dbReference type="STRING" id="36166.T1GF83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002015-RA">
        <property type="protein sequence ID" value="MESCA002015-PA"/>
        <property type="gene ID" value="MESCA002015"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1262834_0_0_1"/>
    <dbReference type="OMA" id="QITCAIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF185">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002015-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="24681" checksum="6B2A91CECCC9962A" modified="2013-10-16" version="1">MVVGECIAPEIIPIAIGVSAVIAGVVFYHIFFNKKSANKSVRSNRTTFHLIATINGELVIRPYTPISSDDDQGFVDLVVKVYFKNVNPKFPDGGKMSQYLEEMKIGDTIAFRGPSGRLQYLGNGAFSIKKLRKDPPKTVKAKRVNMISGGTGITPMLQLARDIMKHNDEDKTQCSLLFANQTEKDILMKKELDELAAQYPDQFKVWYTVDRAEEDQYFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFP8</accession>
    <name>T1GFP8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133909">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002195-RA">
        <property type="protein sequence ID" value="MESCA002195-PA"/>
        <property type="gene ID" value="MESCA002195"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834069_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002195-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7498" checksum="E83D0667E182A71A" modified="2013-10-16" version="1">MSIPLQVSSSKFPNNAWKLTQLVQFLVVHYSPLTLLATSIRSLKRGLMNIFSKMYNPLNLSLLFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFS4</accession>
    <name>T1GFS4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">60S ribosomal protein L10a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFS4"/>
    <dbReference type="STRING" id="36166.T1GFS4"/>
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        <property type="protein sequence ID" value="MESCA002224-PA"/>
        <property type="gene ID" value="MESCA002224"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199447_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002224-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7940" checksum="ECF7677BE7BEFD6F" modified="2013-10-16" version="1">TDKKRNFLETDELQIGLKKYDPQKDKRFSGTVKLNHIPRPKMKVCILGDKQHCDEAKQNNIEWMLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG11</accession>
    <name>T1GG11_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cupin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG11"/>
    <dbReference type="STRING" id="36166.T1GG11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002315-RA">
        <property type="protein sequence ID" value="MESCA002315-PA"/>
        <property type="gene ID" value="MESCA002315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068137_2_0_1"/>
    <dbReference type="OMA" id="SHIDHVP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF13">
        <property type="entry name" value="LD14533P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24641" checksum="A25356DDEF6A157B" modified="2013-10-16" version="1">SIDGPLVLQNITRSDFAPFAYSSQPIIIKNAVAHWPALKLLNYSYLKDLYEKVPGAMDEDCQFLHFRSADIKSLKDFFSMPKSRVEMKDGSDLTWYVGWSNCHPVVLEELRKLYPRPHFLPEDAEMPNTDYIFLGFEQGAFMHLDYIPRLMWQAQLQGNKTWVLAPTPECDSVCHTFSFYVEPGDAVLVDTRMWYHGTSIPKGQFALTIQSEYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG23</accession>
    <name>T1GG23_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG23"/>
    <dbReference type="STRING" id="36166.T1GG23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002328-RA">
        <property type="protein sequence ID" value="MESCA002328-PA"/>
        <property type="gene ID" value="MESCA002328"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013563_0_0_1"/>
    <dbReference type="OMA" id="HAWRRNH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06720">
        <property type="entry name" value="PDZ1_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06793">
        <property type="entry name" value="PDZ2_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000007">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000017">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051230">
        <property type="entry name" value="APP-Binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345">
        <property type="entry name" value="SYNTENIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345:SF16">
        <property type="entry name" value="X11L, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="71"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="162"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002328-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="27289" checksum="15F8BD362B4DBCDA" modified="2013-10-16" version="1">MICHVFESEEAQFIAQSIGQAFQVAYMEFLKANGIEDHSFVKEMDYQEVLNSQEIFGDELEIFAKKELQKEVVVPKAKGEILGVVIVESGWGSMLPTVVIANLMSTGAAARCGQLNIGDQLIAINGLSLVGLPLSTCQTYIRNAKNQTAVKFTVVPCAPVVEVKIKRPNTKYQLGFSVQNGVICSLLRGGIAERGGVRVGHRIIEINNQSVVAVPHEKIVNLLATSVGEVSFLNYFILQKHIFLNLQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGR2</accession>
    <name>T1GGR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGR2"/>
    <dbReference type="STRING" id="36166.T1GGR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002588-RA">
        <property type="protein sequence ID" value="MESCA002588-PA"/>
        <property type="gene ID" value="MESCA002588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_531667_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF60">
        <property type="entry name" value="STRIPE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="480"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="513" mass="55081" checksum="B158DB19854773D9" modified="2025-10-08" version="2">MKSVSQSSALSSTPNITSPVTASNFILNQQSLPQQSASSVRTSVTSSGGAAEAEGSIPNLKRPISVLKQQQQQPSNHLSLIPTVSASTNQKNKDNLEDLNTPISTSSIPSFFGPSSVIEPPPITGSIESEDLGLEVNSSELCSNLKSERTTPRLIVKEESSNINSSYNSNSSTMYPTQSNQNSNNSNTLNNISSNSPHHSISQSTGATMTGINFNVGVGATSAATATVPVSSMNPVQSSDATQVTVFPPQISPSLGLGNNWVLPSPDKSLFQPPLFSLIGATNSNSHLTTHAPSPSPSHNIPLVPFSSSSNSDKQPPIELLGLNMDCSNLLIKQPMPNYSGHHGDIQNQHGCHDVYGGSRQHLGTSIVPKYQWLEATPDYGQLVVGSPTTSNTSYGVIPKQETAQSFSSPTNIPNNMGQSQSNENYSAVQLAEYTPSTSKGHEILSQVYQQSSLPLKLVPVKPRKYPNRPSKTPVHERPYACPVENCDRRFSRSDELTRHIRIHTGQKPFQCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGT7</accession>
    <name>T1GGT7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199213">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGT7"/>
    <dbReference type="STRING" id="36166.T1GGT7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002618-RA">
        <property type="protein sequence ID" value="MESCA002618-PA"/>
        <property type="gene ID" value="MESCA002618"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323054_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042051">
        <property type="term" value="P:compound eye photoreceptor development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052097">
        <property type="entry name" value="SET-MYND_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165:SF7">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002618-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11215" checksum="56820C4902A711BF" modified="2013-10-16" version="1">MKESGNAAFKAKKWLDAMILYSQSLIAVPLKENEEFASIVVANRSAALYHMNKFEDCLKDVARAFKLGYPKDKSYKLYEREAQSHLGLKNNEPAIAAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH01</accession>
    <name>T1GH01_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicastrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nicastrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02069696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH01"/>
    <dbReference type="STRING" id="36166.T1GH01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002683-RA">
        <property type="protein sequence ID" value="MESCA002683-PA"/>
        <property type="gene ID" value="MESCA002683"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024257_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007220">
        <property type="term" value="P:Notch receptor processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041084">
        <property type="entry name" value="Ncstrn_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008710">
        <property type="entry name" value="Nicastrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092:SF0">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18266">
        <property type="entry name" value="Ncstrn_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05450">
        <property type="entry name" value="Nicastrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Nicastrin small lobe" evidence="10">
        <location>
            <begin position="102"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002683-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="645" mass="73369" checksum="6263B40A809BEC24" modified="2013-10-16" version="1">MKKLHSYIHDEHEPSNRFCLVYIAFPPRPVLSQVLQMNKSNLIVTFVGNDGNFIGHILSIDNELDVNHFGKTRRPNVSKHNWSYLFSTLECNPSDRVFLQRGRQHGVLYVIEKQQDFEYLLNSPTSPPYAPVVPPELFTRENLIKLKSAAKNISVVVLINKFDKLSSFSHELACPNQYSGINGTQGSCDLSRPETLWNPQGTGLMHEDFPFPIHYVADPSEIEKIVKCFKKFNDFEYENHPMRSLCSIEVKSFMSAAVNSEICIRRSNFINNLSPVKYCDPLEGKNIYATLFPRPQSVERVIDSNEKVILLTCRMDSTSMFDEVGLGAKDSLASYATLVSVAHMLAKILPNNSGITKNVMFVVFNGESYDYLGSQRFVYEMKKNNFPYVHTKTRPLTLENIDFMIDLGVLDDFDNLDIYTLTDTSKITQSFLREEQKFPVGTLSSKPSNAFYHSIYDDTVNLMNYTYKNKSLDFTTLMSKDEALKYFNETDVQMKVRNVASVIAITLYESLAGKTYQGNEFVNPILVDEFLFCFLESQDCPLFRASLRYTKGVILPAIPPPRYVSVANALRDAAIWSYRILGLLVSQKVEGKAMENCTIPPLNWYSGFSGAGECRLTTQNMTHARSPAFDIEELITNIVDIVKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH13</accession>
    <name>T1GH13_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002696-RA">
        <property type="protein sequence ID" value="MESCA002696-PA"/>
        <property type="gene ID" value="MESCA002696"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2948604_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002696-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6829" checksum="00910170102D474D" modified="2013-10-16" version="1">MYMDNNTPNSSSDAESANEAFMGMGTLDFIIIGVIALGSIWYFFLRNQKKEEEPIRTYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH46</accession>
    <name>T1GH46_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094066">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH46"/>
    <dbReference type="STRING" id="36166.T1GH46"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002731-RA">
        <property type="protein sequence ID" value="MESCA002731-PA"/>
        <property type="gene ID" value="MESCA002731"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127267_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045197">
        <property type="entry name" value="NUP210-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019:SF0">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN GP210-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002731-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12450" checksum="1BEB795AC2BBE9C3" modified="2013-10-16" version="1">IPSASTTSQELDNCCWFQHSNISPRWSSSRCKHYLFTMESTLITGIKVGNTRVTGKCVGINPSTGTEIVFSEDSVEVHVVPIEAIKIRTPLTRIKSGAVMPATIWGSPDLSPMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH75</accession>
    <name>T1GH75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH75"/>
    <dbReference type="STRING" id="36166.T1GH75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002765-RA">
        <property type="protein sequence ID" value="MESCA002765-PA"/>
        <property type="gene ID" value="MESCA002765"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026811_4_0_1"/>
    <dbReference type="OMA" id="GAGCLTM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042805">
        <property type="term" value="F:actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060537">
        <property type="term" value="P:muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09326">
        <property type="entry name" value="LIM_CRP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000001">
        <property type="entry name" value="Cysteine and glycine-rich protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF35">
        <property type="entry name" value="MUSCLE LIM PROTEIN MLP84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002765-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="10850" checksum="102C2DF12C6B1963" modified="2013-10-16" version="1">NSNNYIQNFSPDGRPFAGAKSANGCPRCGFAVYAAEQMISKSRIWHKRCFYCSDCRKSLDSTNLNDGPDGDIYCRNCYSRNYGPKGVGYGLGAGALQMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHA1</accession>
    <name>T1GHA1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamate synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHA1"/>
    <dbReference type="STRING" id="36166.T1GHA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002791-RA">
        <property type="protein sequence ID" value="MESCA002791-PA"/>
        <property type="gene ID" value="MESCA002791"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_12_0_1"/>
    <dbReference type="OMA" id="GESHINE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015930">
        <property type="term" value="F:glutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000314">
        <property type="entry name" value="Uncharacterized protein, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate synthase central-N" evidence="3">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamate synthase" evidence="2">
        <location>
            <begin position="299"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamate synthase" evidence="2">
        <location>
            <begin position="528"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002791-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="732" mass="80803" checksum="4730E1D053767D44" modified="2013-10-16" version="1">MGNDAPLACLSTFQPLPYEYFKQLFAQVTNPPIDPFREKIVMSLQCPIGPESNLLKPSKDQVHRIWLRNPILSITDTDMLKNNKYRDWKTKVINTTFNFKEGAEGYINCIRRICKEGQQAAKNDYQLIVISDRVVDEKNVPVSALLALGALHHHLIETRLRMKVGIIVESGEPREVHHICVLLGYGADAICPYLAFELAQSLRDDGVLDLNMTNEDIYSSYSQAIETGISKVMAKMGISTLQSYKAAQIFEADRWSKLNVLACEGLTRYMMAYPEIEKDSKILRNPGHYHWRHGGESHINEPGAIAALQEASTKNNKNAFEQYRSITHDSVKKCTLRGQLEFQYSENKIDISEVEPASEIVKRFATGAMSFGSISLEAHESLAITMNKIGGKSNTGEGGEDAERYLDQDPLNNKRSAIKQVASGRFGVTASYLANADDLQIKMAQGAKPGEGGELPGYKVTKDIAKTRKSVPGVGLISPPPHHDIYSIEDLAELIYDLKCSNPNARISVKLVSEVGVGVVASGVAKGIKNAGLPWELGIAETHQVLVLNNLRSRVIVQADGQLRTGFDVVVAACLGADEFGFSTAPLIVMGCTMMRKCHLNTCPVGIATQDPVLRKKFAGKPEHVINFFFMLAEDIRSIMANLGVSKFQDLIGRTDFLKPSENRSLKGNMLDLSLLLKPALELRPNTNIIGGSVKQNFELEQRADNDLINKVQSPPPLMPPGPGRILSLFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHC8</accession>
    <name>T1GHC8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHC8"/>
    <dbReference type="STRING" id="36166.T1GHC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002823-RA">
        <property type="protein sequence ID" value="MESCA002823-PA"/>
        <property type="gene ID" value="MESCA002823"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183246_0_0_1"/>
    <dbReference type="OMA" id="KSTIPQC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF3">
        <property type="entry name" value="ARFGAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002823-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7696" checksum="5DC91774B149495F" modified="2013-10-16" version="1">MPELIQVYDFIASAKEDYDSPTTSTFCKSTIPQCRQTLQILDERLEYDKEGLTKLKKAVKAVHNSGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHG2</accession>
    <name>T1GHG2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002859-RA">
        <property type="protein sequence ID" value="MESCA002859-PA"/>
        <property type="gene ID" value="MESCA002859"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560876_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002859-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9067" checksum="99CD3C8F9ACE9CB5" modified="2013-10-16" version="1">MVWVYTFNPVFSLIQSKKLVTLTRTAGASPSQPPPETKEFIPICSYVSKVTKGPPESPLQIDFPPVATQMLSSSTVSSKYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHP7</accession>
    <name>T1GHP7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="5">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02131679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHP7"/>
    <dbReference type="STRING" id="36166.T1GHP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002955-RA">
        <property type="protein sequence ID" value="MESCA002955-PA"/>
        <property type="gene ID" value="MESCA002955"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_0_1"/>
    <dbReference type="OMA" id="KEHEWIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="16"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="4">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002955-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13700" checksum="A3EAC15279649895" modified="2013-10-16" version="1">RLYTKKHEWVQVEGDIGTIGISDHAQEALGEIVYAQLPDVDTKFQQSDECGALESVKAASEVYTPVSGVVTEKNTAVEESPGLINTSCYKDGWLFKLKLSDSGELKKLMNESQYQEFLKTDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHT3</accession>
    <name>T1GHT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL21m</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02163523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHT3"/>
    <dbReference type="STRING" id="36166.T1GHT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002994-RA">
        <property type="protein sequence ID" value="MESCA002994-PA"/>
        <property type="gene ID" value="MESCA002994"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061463_5_1_1"/>
    <dbReference type="OMA" id="NFTNAFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_bL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_bL21"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002994-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="23631" checksum="A6FCB060085418B4" modified="2013-10-16" version="1">MSFLMKNFASLTRTAASPNFTNAFRNLSITSQLSVRQNLNYKPPKIIAKPITPILPQRSPSEDQKGCSNVISQVNKVIAEKTEGRLFAVIHLCGKQFKVTAGDIILVEGYWPPTIGDQIRLEKVLLVGGNDFTLIGRPIVDKNLVDVQATIVEKTLTHTKTHFKKKRRKQYMRINFQRSPNTMIRINSIDIIGQLDQTPLEPEDPTKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIJ3</accession>
    <name>T1GIJ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIJ3"/>
    <dbReference type="STRING" id="36166.T1GIJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003272-RA">
        <property type="protein sequence ID" value="MESCA003272-PA"/>
        <property type="gene ID" value="MESCA003272"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_14_5_1"/>
    <dbReference type="OMA" id="CAICEAF"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF475">
        <property type="entry name" value="KELCH-LIKE PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="2"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="BACK" evidence="5">
        <location>
            <begin position="87"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003272-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39223" checksum="DEC389F4F3E079A5" modified="2013-10-16" version="1">MRDSDLLQPQETLKTRRQLERHQGVVTLHDVDVEALRQLIEYTYTGELTITEDNVQVLLPASSLLQIQSVREACCKFLLRQLHPSNCLGIRSFADAHSCKELHSRSHKYALQNFQQVVSTEEFLLLPFDEVRDLISNNQLNICSEEKVFLAVLNWVKHDLVERKKHISELMGHVRLPLVSREFLMSCVESEGLIREDSHCKELLLEAMKYHLLPEQRSLMSNKRTIERRPDGMKPYVFAVGGGSLFAIHNECEVYNPRTGNWSSISPMLWKRSRMGVTSLHKQLYVVGGYDGVSDLASAEFYNPLNNRWNYITPMGTKRSCLGICAFDGLIYLAVDMMGHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIL5</accession>
    <name>T1GIL5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CVC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIL5"/>
    <dbReference type="STRING" id="36166.T1GIL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003296-RA">
        <property type="protein sequence ID" value="MESCA003296-PA"/>
        <property type="gene ID" value="MESCA003296"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867436_0_0_1"/>
    <dbReference type="OMA" id="HHQKGPV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023339">
        <property type="entry name" value="CVC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052294">
        <property type="entry name" value="VSX_homeobox_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46892">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46892:SF3">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51496">
        <property type="entry name" value="CVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CVC" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003296-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15221" checksum="8006E99C7759DDE6" modified="2013-10-16" version="1">MAEYGLYGAMVRHSLPLPDTILKSAKEDESVAPWLLGMHKKSIEAADILKSDESDRETPTSDDTNTSYSAGSTHNSPISSFSISRLLFDGQSSNNNQHNHHHHHHHQKGPVKPAPPLPLHNLKQETHQQPLQAPESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIY9</accession>
    <name>T1GIY9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIY9"/>
    <dbReference type="STRING" id="36166.T1GIY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003426-RA">
        <property type="protein sequence ID" value="MESCA003426-PA"/>
        <property type="gene ID" value="MESCA003426"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105020_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003426-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="28916" checksum="2E927FFB76997778" modified="2013-10-16" version="1">EIIKDSIDTDEENDVSTIKAKDKRRISFAETDQVKYIDNKESVDSMFPKPEKDVIKLDLPVLKPEPVIVDKKKAILEKVEQNLKFQEENSSLSDFNMVDKIISNSKGKTLEIKFTNSKTEVQNKKIEGVVNDPSKLIEFLSGNNLENEEIKIPFLSEDRRPEAYEDVRAKFSVPSKSILKNREYVPPKEEVVEVPKNVKKQDTQKYIGDFSMIMGDVVENTIPDPLPEKFVDANAPKKRISKFKQRKMGIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ08</accession>
    <name>T1GJ08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02033189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ08"/>
    <dbReference type="STRING" id="36166.T1GJ08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003447-RA">
        <property type="protein sequence ID" value="MESCA003447-PA"/>
        <property type="gene ID" value="MESCA003447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_516098_0_0_1"/>
    <dbReference type="OMA" id="NCENEIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042063">
        <property type="term" value="P:gliogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000061">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000064">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000230">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF10">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 2 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="53"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="133"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="190"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="228"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="266"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="528" mass="57789" checksum="83AA46AC35496F97" modified="2013-10-16" version="1">MKIPNNLLHCKPGWKGALCDECETHPSCLRGYCTLPGTCICNEGWGGQFCNQDLIYCTHHKPCKNNGTCFNTGEGYYTCKCEPGYFGDNCENEITTCMDNPCANNGKCIDEISKNTYRCECPPGWKGKNCQQQIITCSEKPCMNNGKCLPRNNGFHCDCTQGFGGQTSRNSTGGYRCDCIGDYIGDRCELYDLCATNPCENGGTCLLKGNQFQCKCKPGFIGSMCSDEVNLCIAKPCANGGTCRNLDNDYQCTCRSGFTGKDCSIDIDECSSAPCKNGGTCVNRINSFECLCPHGYEGKECEFESYSASYGAQLYDTQSSSMNKDDGLSSGQVVLIAILSVAMPVVAVIAACVVVCIKRKRKRDQDKHDAEARKQNEQNATAHHHIHTHRGSNSGLPFDNSNPNIIKNTWDKSVNNMSSSNASVEDTLLNSSLYGAGTYSDNNNANGEAFMVVPSLQRVKSQKQLNTDPTLMHNHRASQMLSQQQSQQAHLMDSKRLSVWVVWVMVSNVTNSNNNSGGVNKMEPLAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ64</accession>
    <name>T1GJ64_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ64"/>
    <dbReference type="STRING" id="36166.T1GJ64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003508-RA">
        <property type="protein sequence ID" value="MESCA003508-PA"/>
        <property type="gene ID" value="MESCA003508"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770181_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003508-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16650" checksum="00CD22F958FFBC20" modified="2013-10-16" version="1">MPYTVNGPPPDRCKLCAIRVILWQSSQTLSVVQQINALKSGIQKVRGIIVTHNGRTIRYLDLLNKANDTIQLDLTNATTVVKRERHSRSFNTVHIKDIQDRSRSRSFEQGQRHHSIGLDHWISKDTSSSPVCAKSPSLERVTSLRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJN3</accession>
    <name>T1GJN3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Chromodomain-helicase-DNA-binding protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02105483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105486">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105489">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GJN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003683-RA">
        <property type="protein sequence ID" value="MESCA003683-PA"/>
        <property type="gene ID" value="MESCA003683"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000062_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18663">
        <property type="entry name" value="CD2_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17995">
        <property type="entry name" value="DEXHc_CHD6_7_8_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051493">
        <property type="entry name" value="CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056342">
        <property type="entry name" value="HTH_CHD6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850:SF1">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23078">
        <property type="entry name" value="HTH_CHD6-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00592">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160481">
        <property type="entry name" value="BRK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="1552"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="1653"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1943"/>
            <end position="2099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="79"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="591"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="775"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="983"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1294"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1383"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2192"/>
            <end position="2228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2332"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2467"/>
            <end position="2544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2608"/>
            <end position="2636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2886"/>
            <end position="3073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="3225"/>
            <end position="3272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="3405"/>
            <end position="3426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="3549"/>
            <end position="3591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="3694"/>
            <end position="3747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="3783"/>
            <end position="3830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="3855"/>
            <end position="3896"/>
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</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJX1</accession>
    <name>T1GJX1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Laminin G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02123219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJX1"/>
    <dbReference type="STRING" id="36166.T1GJX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003780-RA">
        <property type="protein sequence ID" value="MESCA003780-PA"/>
        <property type="gene ID" value="MESCA003780"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1564650_0_0_1"/>
    <dbReference type="OMA" id="FRNWHIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000134">
        <property type="entry name" value="Slit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF85">
        <property type="entry name" value="SP2353, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Laminin G" evidence="2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003780-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="19323" checksum="1333F268694E497E" modified="2013-10-16" version="1">MEPLRTKPEANVTIVFSSTQQNGILMYDGNNEHLAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHSVELLAIKKNFTLRVDRGLARSIINEGSKDFLKLTTPLFLGGLPSEPGQQAFRNWHIRNLTSFKGCLKEVWINHKLVDFGNAARQQKINPGCLTKTDTIDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJX2</accession>
    <name>T1GJX2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJX2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003781-RA">
        <property type="protein sequence ID" value="MESCA003781-PA"/>
        <property type="gene ID" value="MESCA003781"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760703_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003781-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8032" checksum="B42AE5DAB2167A34" modified="2013-10-16" version="1">MEWKGTGRIFNITATKTKVNKESGEFITELEDIRPASSDDDYETGTGTGNVLDYETNICSWNNKLHYFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJZ4</accession>
    <name>T1GJZ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJZ4"/>
    <dbReference type="STRING" id="36166.T1GJZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003804-RA">
        <property type="protein sequence ID" value="MESCA003804-PA"/>
        <property type="gene ID" value="MESCA003804"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357668_0_0_1"/>
    <dbReference type="OMA" id="VQKWYEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="89"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003804-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="22768" checksum="51F4588E47D323AC" modified="2013-10-16" version="1">ALGFLNTFLEGQTYVAGENFTIADISILATVSTFVLAGIDLSPFPNVQKWYELVNKTAPGTELNQQGLDEAKKWFDKKYGKDDSLYPKEAKKRAVVDQRLYFDMGTLYQRFAEYFYPQKLEEALGFLNTFLEGQTYVAGDNLTIADISILATVSTFVLTGINLSPFPNVQKWYELVDKTAPGTELNQEGLVEAKKWFDSVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJZ8</accession>
    <name>T1GJZ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003808-RA">
        <property type="protein sequence ID" value="MESCA003808-PA"/>
        <property type="gene ID" value="MESCA003808"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362114_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577436" evidence="1">
        <location>
            <begin position="19"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003808-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10874" checksum="AB5E99188CAAEFD7" modified="2013-10-16" version="1">MIRLICVVLGFFNCGILAMPPCYSRCTNPHTVLITDNICIRDTLTGIEFTYPNICGLIVLNCLGTMSNRYQEIDYYICHAKMSRPFSLPLRADNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLJ7</accession>
    <name>T1GLJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLJ7"/>
    <dbReference type="STRING" id="36166.T1GLJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004402-RA">
        <property type="protein sequence ID" value="MESCA004402-PA"/>
        <property type="gene ID" value="MESCA004402"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_4_1_1"/>
    <dbReference type="OMA" id="MAITNIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004402-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28626" checksum="3B2818BE5C10D02F" modified="2013-10-16" version="1">MSCNEITIAILIVILLVLYLTRSPDLIPGWEKVIGESMQIKGALPTYLIVLAIYILPSSYKFLRNCHWGTSNIPRRVPPLMTWEYINLNIPWSLMFVVGAGLATVKANNVSGLIPKIADRLLLRVTIKPLELQIFEIIVVSIFLTAFSSNVSTAYQLFELLAKISNAAVKHPILIFYPAVLGCSMAFHLPVSTPPNAIACGYGNIRTKDIILSGIGPTIAAIILLETFGLFWAEVIYGKGVISVWPEWANSFNVSYSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM77</accession>
    <name>T1GM77_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004645-RA">
        <property type="protein sequence ID" value="MESCA004645-PA"/>
        <property type="gene ID" value="MESCA004645"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1505162_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004645-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="20597" checksum="C7AFE193C332A287" modified="2013-10-16" version="1">MLIREKLKLKYNFEQFLEPDKKKTSKNPHNLVDKQQVGGDDRAGVEDLFLYDVVIVNTHIDRFHRFTRGEIETNWVRVETVFFSQFNNSVLSIVTTVDSQSFWDNKERFSESLNSQTHLAFDGVLVFHKSVIGTNLKSSSTWNDTLVFDGVVYSAETISDGVLDLKKSVLVWSLHQNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMB6</accession>
    <name>T1GMB6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongator complex protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMB6"/>
    <dbReference type="STRING" id="36166.T1GMB6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004686-RA">
        <property type="protein sequence ID" value="MESCA004686-PA"/>
        <property type="gene ID" value="MESCA004686"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092581_0_0_1"/>
    <dbReference type="OMA" id="ISCKWLA"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004686-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="21020" checksum="3E94B79B6EA9A88D" modified="2013-10-16" version="1">MAASLLIACGLQEQKLPKHIHISEESSIDGSFLISSILGQRVRVPNSKTLLVCLHQNFKHYLNAGLRLGYNLQMHNGKSLTVIDVVNDIGTQPISCKWLADNPTETLYKAICDELAKLQGSVTVIIDNLEGLMNLGASAQDLYTLCVKLQKFLKESSFESHTLATKLNNCNLFEMTDTNLEHLSELHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMG2</accession>
    <name>T1GMG2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02007000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMG2"/>
    <dbReference type="STRING" id="36166.T1GMG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004736-RA">
        <property type="protein sequence ID" value="MESCA004736-PA"/>
        <property type="gene ID" value="MESCA004736"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_1"/>
    <dbReference type="OMA" id="GRFGFNM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF119">
        <property type="entry name" value="FI03731P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="5"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="89"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004736-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="31513" checksum="A0149AF7EB269D0A" modified="2013-10-16" version="1">KFQKVVVCNNIPPTREDTLQKIQGVDGVLWAHTDKVNGEVLDAAGKQLKVISTMTSGIDYVEIPTVKERGVLLGNTPGAVNVCVAELAIGLMLAAGRRFQHGRRIIETSTWETNHIDWMYGQEIRGSVVGFFGFGGIGQAVAERLKPFGVKKMITIQEGSFQMKTNLEQVIESDFIVVASPLTEQTREIFNEAAFNKMKKNCVFVNVGRGQIVEQNSLIKALKEGKIFSAGLDVMTPEPLPASHELMTLENCVVIPHLGTATLQTTTDMALIAAQNILNGLEGIPLPAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMS6</accession>
    <name>T1GMS6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004856-RA">
        <property type="protein sequence ID" value="MESCA004856-PA"/>
        <property type="gene ID" value="MESCA004856"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530066_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004856-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9441" checksum="1F47AA2CCD099773" modified="2013-10-16" version="1">MVQYALYFSQLTNFKIPSLAKLGQISRENRRILNLRDLHGILKISYIILDNYKGISEPPTSKANSNNVSTSSISPGMSDKQLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN25</accession>
    <name>T1GN25_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase RNF10</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING finger protein 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNF10 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02392567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN25"/>
    <dbReference type="STRING" id="36166.T1GN25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004964-RA">
        <property type="protein sequence ID" value="MESCA004964-PA"/>
        <property type="gene ID" value="MESCA004964"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848809_0_0_1"/>
    <dbReference type="OMA" id="NTENAQY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16536">
        <property type="entry name" value="RING-HC_RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004964-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="328" mass="38499" checksum="EFA1902A09C49B92" modified="2013-10-16" version="1">KLSVCYKWVCKYERNNFVADTLIDWNLIEQINIQTTEEQQCPICLFPPIAAKLTRCGHVYCWPCILHYLALSDKTWRKCPICYEAVHSLDLKSTAIIQKSNFNVGNEISFQLMRRKKGSIVVEKANTKEKVLDKIPHLSEMDESRLFSKFLISDLDEILTIIEREKTELLFDNDPSCPEFIFIQQALDLLEQRKNEVLKNTDILKMHPKLEETLEENDGERYYYFYQSIDAQNIFLHPLNVKMLSSMYSSLSDAPDIIKGNILQKESQSMDDILRKKFTCLGHLPLTCQFEIVEIDIKPPYISKEIIEVFKGNFLFINIFLFFLFCFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNA5</accession>
    <name>T1GNA5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="T1GNA5"/>
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        <property type="protein sequence ID" value="MESCA005049-PA"/>
        <property type="gene ID" value="MESCA005049"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962110_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF56">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005049-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="15309" checksum="AF981939A684AF1E" modified="2013-10-16" version="1">MSYYGSPGLDLNFFFNTSVQLSVLKDKRTSLEEEYYNQLQMSLKKLDFDRIPTLKAIQQEILDKEFYGFWAMVQSFPMTSFSRDDTNIELYNDMNEIHLKRKMMFSSNRMTDTLKYSLLRFDELGIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNA7</accession>
    <name>T1GNA7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNA7"/>
    <dbReference type="STRING" id="36166.T1GNA7"/>
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        <property type="protein sequence ID" value="MESCA005051-PA"/>
        <property type="gene ID" value="MESCA005051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1273521_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24195" checksum="53D06C360A664427" modified="2013-10-16" version="1">MKCKCKNWSNNLNNFVVRKLKYFKISGQQVLINKMPAMPKEKTSPLTLENSFSDKKVKEQYVPHDCNTTKNEMAKESNNSTDNKEMISTMAVSSDSVLSPKKQPNNLRKYIDDCKINSSKDGCLSSPPQTFKSVVSANNNISAVPARNQNQSQQSTKSTTTTTSSPTATLANNAKTPSKKTANDFIFGKYIGEGSFSTVYLAREINTHREYATRVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNA9</accession>
    <name>T1GNA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02065763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005053-RA">
        <property type="protein sequence ID" value="MESCA005053-PA"/>
        <property type="gene ID" value="MESCA005053"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925234_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005053-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7065" checksum="742D6DB297D63000" modified="2013-10-16" version="1">MDDNAAALCRQRARWVDLHGNQQLETVNSWRHTAGFSRRIINNKVNFMEKFGENSSKCLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GND0</accession>
    <name>T1GND0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GND0"/>
    <dbReference type="STRING" id="36166.T1GND0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005077-RA">
        <property type="protein sequence ID" value="MESCA005077-PA"/>
        <property type="gene ID" value="MESCA005077"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2819934_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005077-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7560" checksum="D5E870325EA1E50A" modified="2013-10-16" version="1">MALSRRQKSWDILDQSALSFARHHKVQQHHQEEQGSRSSVSNESNVLLDPEVLPDLSIQALVLTILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNH0</accession>
    <name>T1GNH0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02053947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNH0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005121-RA">
        <property type="protein sequence ID" value="MESCA005121-PA"/>
        <property type="gene ID" value="MESCA005121"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778752_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005121-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7812" checksum="7C44E0A286248702" modified="2013-10-16" version="1">MRTSHICWTFATCASVQYILNIRHLCESPITIGNSPKPIQNHTAGVVLTFGHEYISMPVCFVWGFLLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP98</accession>
    <name>T1GP98_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP98"/>
    <dbReference type="STRING" id="36166.T1GP98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005420-RA">
        <property type="protein sequence ID" value="MESCA005420-PA"/>
        <property type="gene ID" value="MESCA005420"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2099662_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000863">
        <property type="entry name" value="fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005420-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="12817" checksum="5B1BDCBDD1925947" modified="2013-10-16" version="1">MHTHNDKKPYTCKVCAKGFCRNFDLKKHMRKLHDTNGNSSRKAQHHQDPELCSSPLSHSPPINVTSPSPSSVDSSSTSWHSSALVTGLPSYAANSFMHLANNTNQRMENVPFIAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPR1</accession>
    <name>T1GPR1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tox-GHH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02100809">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPR1"/>
    <dbReference type="STRING" id="36166.T1GPR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005602-RA">
        <property type="protein sequence ID" value="MESCA005602-PA"/>
        <property type="gene ID" value="MESCA005602"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707617_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tox-GHH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005602-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8678" checksum="6FFD2F19FEB1753E" modified="2013-10-16" version="1">MHHAKLTAVRKAWHREKEILRNGLTSGIEWTQQEIDEILKQGYANNYEGEYIHDVSLYHELSEDPYNISCKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPT2</accession>
    <name>T1GPT2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPT2"/>
    <dbReference type="STRING" id="36166.T1GPT2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005625-RA">
        <property type="protein sequence ID" value="MESCA005625-PA"/>
        <property type="gene ID" value="MESCA005625"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_2_0_1"/>
    <dbReference type="OMA" id="QYMHENH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000076">
        <property type="entry name" value="phospholipase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588446" description="Lipase domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="7">
        <location>
            <begin position="69"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005625-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="353" mass="40533" checksum="FA9CB8DA3193187B" modified="2013-10-16" version="1">MERFQVFLLILSISFDVIYGDEDLSDILEAEVEDDQIWRVPQFDGTFKMMTEQEAKDITLRTEVRGRFKNITNLLFKDKRKKVQFFLHTQKNIIKWQEIDMERPETLHRSFFNKNHPTRIFAHGWLSGMPQMQSYEIIDSHLLNGRRENYNIIIVDWSVLGKTLNYYAARLRTRKAGLKVAQFIDWLHTEGGLDFETTHVYGHSLGAHVAGFTGKQVTKGRIKTIVGLDPAMPFFRTKNPQNRLADTDAEYVETIQTNGRELGFYHPIGQASFYPNGGLRQPGCGKDYLGGCAHIRAPTFLAEAIARGKHNSFDTVKCKDWTEMREKKCAGNSEGVRMGDPENYKKAKGNNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQJ3</accession>
    <name>T1GQJ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005902-RA">
        <property type="protein sequence ID" value="MESCA005902-PA"/>
        <property type="gene ID" value="MESCA005902"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309178_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005902-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11479" checksum="1C38FBB94A7DD7E8" modified="2013-10-16" version="1">MTVHKICFTSTPKTKKNKETTYEMAQIKSCSPIPKDLYQFSNQILYAATCHQEPTYDRSLTRSCTSSKKTVKNLNLPMTVIKRYLVLLPQRSSSQNQSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQK0</accession>
    <name>T1GQK0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02057369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02057370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02057371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQK0"/>
    <dbReference type="STRING" id="36166.T1GQK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005909-RA">
        <property type="protein sequence ID" value="MESCA005909-PA"/>
        <property type="gene ID" value="MESCA005909"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009579_3_7_1"/>
    <dbReference type="OMA" id="YESFICI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF170">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005909-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26633" checksum="027782C6E94A5CE9" modified="2013-10-16" version="1">MRHVDKIFTPHFCPSRSPINPCSTYQIDVSFYVTLKPPSRCQCWMAFTWISAGLLCCPPLLGYSVPLYNKEAYVCMLDWGNMAAYSATLGILVLGPSVISIVHNYGYIFVMMRRLRSGEPIHDKEYATALAENLANPSHMMSFVLVFAFWASWLPLIFVRIYQGITGYVIQNTMVNFGLVWIGVLNSFWKIVIMTAMSPQFRIALRVFCLTICCKTKGRLQAELIGLDPDDWSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR00</accession>
    <name>T1GR00_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02100822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02100823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006068-RA">
        <property type="protein sequence ID" value="MESCA006068-PA"/>
        <property type="gene ID" value="MESCA006068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186943_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12276" checksum="0D2CDDB42E010F07" modified="2013-10-16" version="1">MITKAAKIDTKNMINNESTEILGYTDVIDVVGRTTKKEAMILKTDEDLSVFLFPYSNKIPSKRVNCLEGLGSGHDFSQEMIRVFVNFEISKSRSCTLLMFPVNSGSQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRT0</accession>
    <name>T1GRT0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02109128">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRT0"/>
    <dbReference type="STRING" id="36166.T1GRT0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006370-RA">
        <property type="protein sequence ID" value="MESCA006370-PA"/>
        <property type="gene ID" value="MESCA006370"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770183_0_0_1"/>
    <dbReference type="OMA" id="MTIVWRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006370-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="17152" checksum="E8A2FFEB27530662" modified="2013-10-16" version="1">MTIVWREIFPSKFSVIIFILYMALFVNQGILVTFSQNKSNNYEFSTVAVVLLTEVTKLLVSCVLYCKDNTVKSLFSNVQKDFNVLLLYFVPSFLYCLYNNLAFINLSKFDPTTYYLLLQFRVVIVGILFQIKLTILTSFFYYVTQCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRY9</accession>
    <name>T1GRY9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006436-RA">
        <property type="protein sequence ID" value="MESCA006436-PA"/>
        <property type="gene ID" value="MESCA006436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963413_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6949" checksum="4B0D197C3ED0F24E" modified="2013-10-16" version="1">MNNYWGRQTFATCANVQFILDIRHFFFLILLQLFKSTFTYFVADGTLVEKTLTIRIHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSF5</accession>
    <name>T1GSF5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02044163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSF5"/>
    <dbReference type="STRING" id="36166.T1GSF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006610-RA">
        <property type="protein sequence ID" value="MESCA006610-PA"/>
        <property type="gene ID" value="MESCA006610"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1403931_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006610-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="22382" checksum="7ABE01990F03E380" modified="2013-10-16" version="1">MNMILQNRFGITYNELCSITNGYGIICGIQNVAESYYGIPILILFALNFTYIIIELFLFWYASFERPKGQDRPFHRVAVTARIFYNIVQMISISELCQRIAHENRKIGDNLFKILDSKILKNVEEKSLDLLLQVDSRKMEFSAGGVFKINRSLMFTISGAATTYLIILVQFRLANLNQESIMKSLNCTKLPHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT93</accession>
    <name>T1GT93_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT93"/>
    <dbReference type="STRING" id="36166.T1GT93"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006918-RA">
        <property type="protein sequence ID" value="MESCA006918-PA"/>
        <property type="gene ID" value="MESCA006918"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043212_0_0_1"/>
    <dbReference type="OMA" id="WIHENAG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000073">
        <property type="entry name" value="Trypsin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577610" description="Peptidase S1 domain-containing protein" evidence="7">
        <location>
            <begin position="17"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="39"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006918-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="493" mass="53614" checksum="0B5E098AD55E271F" modified="2013-10-16" version="1">MNFLIVLACCLAAASAVDFTPIPEPTPFKLPTIPGNGRITNGQVATRSQFKYQVGLSLRAADGSYWCGGTLISDRWIVTAAHCTDGILSVNVYLGAWNRKDATEEGQQIVFAGQRYIFVHEGWDTVETKNDIALIKLPSPIKFNDYIQPAQLPKYRPDGDYPTYEGVVATASGWGRTEEGTSPDTLRYIEKPIMKLSSCSQKQSTCNGDSGGPLVVEEDGKPVVIGATSFGIILGCQSGWPGVFTRITGYLEWIHEKTAISALGLSEKITNGQVAERNQFKYQVGLRLTGENAIYWCGGTLLSERWVLTAAHCTEGILSVNLYLGAWNRSDPQELANRYYLQGNNDIALIKLPAAVRFNEYIQPAQLPKYRSNGSYSSYEGVPATASGWGVTETGNISDTLQPILKLKSCNRWYLGALTASQQICISVTNKQSTCNGDSGGPLVVQEDGKTVLIGATSFGVVFGCQSGWPGVFTRITGYLPWIHEKTGLVEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU28</accession>
    <name>T1GU28_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392031">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU28"/>
    <dbReference type="STRING" id="36166.T1GU28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007229-RA">
        <property type="protein sequence ID" value="MESCA007229-PA"/>
        <property type="gene ID" value="MESCA007229"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_406889_0_0_1"/>
    <dbReference type="OMA" id="FREYTHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="6">
        <location>
            <begin position="477"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007229-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="676" mass="76439" checksum="18D25E7E0C5B9012" modified="2013-10-16" version="1">QNALRQTKLSFSSNTTIAVPVPEQSSRQKQISNALALFLASEPKVSAEMLTREPFQHLANVLFDGKMLKAAPIQSANTLRKKMMDTFEDIKVKAIQYIKEKAPKVIAVIFDLWTDSSSFSRNFLGIQLQFSLNLEITNINIGTFIMEGPKTGMAIAEIIFKTLQDFGITDRFIFFVTDDGSNCMSAVSILLSQFGIKGERLPCLAHQLHNLVTSVILKKKNVDAVSDVTNVIEKLSKIKTALRWRTQEIVEKDKNLRLIKALSVTLNGSEEDEEALEELLNSQSVSSKVAVNDVLVGKNLDLLISRSDDIVLSDLSIILSPINDFVTKIQSKTNGTLRQVMNGYKKLKDSFQNNDDIGTEAGKMLQLQFGMKLNDRFNIVALYQASQAVEPGMLGWDISLNEGRVLIKNYAKTFEIFSEDIFEEQVTNESAPKSNEDSERISRLKERFALDLVNEKSSKLDIEITAFANLSASEALLNYVEFWEQNKSKFKIIYKLFDLLYGIPATSSSIESAFSVAGNVVNKSNGNLSDEKVAAKCFVKINYQFLQKIGAIPSVKFHNSRKRRAMNRVKTTNKKSIENREMARKDDFVFKSEDFQNVKSFNSLISQHKDDIFERFSIKTQNSINSTKYLSKIWNYFGYLYVTDEEGNSRHVLTKRNFCMECLNDLKNNNGNIFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU41</accession>
    <name>T1GU41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU41"/>
    <dbReference type="STRING" id="36166.T1GU41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007243-RA">
        <property type="protein sequence ID" value="MESCA007243-PA"/>
        <property type="gene ID" value="MESCA007243"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1095342_0_0_1"/>
    <dbReference type="OMA" id="RVFENIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007243-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="29664" checksum="E278614B7E7AF107" modified="2013-10-16" version="1">MQRMLEKLESCETFYLQCQTSEKILSNANVELIEVKPDEFIFDNNETQKQEDESEIEVDALQLEEPKIEDESYPDSSQEDALQDLNQDDQDSDFELSEIDEKPDLPNPEVKIEKPPKTAKVKKADNSMSMCDICGNCFKSARLYSHLRRHNNIKPHVCEICSKAFTTSSELCRHMRVHTGERPFACQYCDRRFTDKSTHQNMKELTLEKGRFRATNAENLSHILRVFENIWLFILAKKIMLVFHVIKHFLGHIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUA2</accession>
    <name>T1GUA2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUA2"/>
    <dbReference type="STRING" id="36166.T1GUA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007312-RA">
        <property type="protein sequence ID" value="MESCA007312-PA"/>
        <property type="gene ID" value="MESCA007312"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036543_0_0_1"/>
    <dbReference type="OMA" id="FIKFCER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007312-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="269" mass="28744" checksum="623404AF96F9F1D5" modified="2013-10-16" version="1">MSCISSNFSSFFLDSLNDPSEFAKYLSNGDNRMVPIVTSTSYDDYNGGQSFHHQNHHHLQNHHPHHHNGTNGVCDSSEMSGTGATSSYNLPPTSYNHQSSTSVTPTNATNAPDKQQAAHPTPQTVIAATAAGATSSQNSQHFDGSFEFLKYLRYSADYGGGEDSVHSIQSNTTNSSLIDLDSTPSSTHKSKGRNGNLELDSTYDHDSKHNIFDLHHQTNSCSSTNNTPHLSASNSNPSSECGSDNSNNLINHNSSSTTTTSSSTTAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUJ8</accession>
    <name>T1GUJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02005771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUJ8"/>
    <dbReference type="STRING" id="36166.T1GUJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007412-RA">
        <property type="protein sequence ID" value="MESCA007412-PA"/>
        <property type="gene ID" value="MESCA007412"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="OMA" id="VAGCQKG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588542" description="Peptidase S1 domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="31"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007412-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="271" mass="29449" checksum="AF392586B6762789" modified="2013-10-16" version="1">NNGNFIRFIIFSSSFLLKTAETAAFSGDARIVGGKRAQLGMFPYQVAVFIYDKENKSYICGGSIIDENWILTAGHCTYDRVRAIVIAATVDLSGPTPNQQVIFVNDTSDLMTHPEYKREKVVNDIALIRLPTSIQTNEYAGVVTLRSNDKSSYIGKNVTASGFGKTSDSSQPSQHLMWTTLTVISNTVCARSYQPGLVDNTKLCTSAGNIFRPHSTCQGDSGGPLVLTETYEQIGLVSFGGDSCQRGTPVAFTRISAHKQFIEGTTGLTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUL9</accession>
    <name>T1GUL9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007435-RA">
        <property type="protein sequence ID" value="MESCA007435-PA"/>
        <property type="gene ID" value="MESCA007435"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243247_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007435-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12202" checksum="750D389CBACAF5A8" modified="2013-10-16" version="1">YVRYHQIGRIRDTLRSINERYRNRDGNDLTTSRDSATATDNNIVMDFEEEEVLDSSDGFTTNEDSLHTSDVETLESMEEDGGDVMDYDENLTGEHKYLEEMERVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUV5</accession>
    <name>T1GUV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4794 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02059900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007526-RA">
        <property type="protein sequence ID" value="MESCA007526-PA"/>
        <property type="gene ID" value="MESCA007526"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534295_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577158" description="DUF4794 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007526-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19963" checksum="2138AB04F4C895D3" modified="2013-10-16" version="1">MKSLVSLFFCATFVLQLVQSQYSADYGWERVLDKPRPIKFFPKPVYIPAKSRKSPGIIDVDGPQRSIKKDKPRNRPHRVIRPVNGTPYIRPKRSPEIIEVDASPVIVPGATYNNQPQARPFRPDIIQVNPHTRPPYGRFPIPRQNFPVPAGDIPSRPWDIGAIPPVSFKYFILTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV08</accession>
    <name>T1GV08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">adenylate cyclase</fullName>
            <ecNumber evidence="3">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02036157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV08"/>
    <dbReference type="STRING" id="36166.T1GV08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007582-RA">
        <property type="protein sequence ID" value="MESCA007582-PA"/>
        <property type="gene ID" value="MESCA007582"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169356_1_0_1"/>
    <dbReference type="OMA" id="CHVETIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF16">
        <property type="entry name" value="ADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577744" description="adenylate cyclase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="13">
        <location>
            <begin position="14"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007582-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10412" checksum="817F1F5D9F92E7C5" modified="2013-10-16" version="1">MSSRKTIVVVCNILILFADICGFTSLSDQCTAEELVRLLNELFARFDRLAAEHHCLRIKLLGDCYYCVSGLPEPRPDHAHCAVEMGLDMIDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV52</accession>
    <name>T1GV52_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02060077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007634-RA">
        <property type="protein sequence ID" value="MESCA007634-PA"/>
        <property type="gene ID" value="MESCA007634"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3147470_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007634-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="49" mass="5520" checksum="9AA6CFC776D9EAD0" modified="2013-10-16" version="1">MAKKKINWVHNCPGTPHSGGAWEILIKSVKRLIDISLKDCIPTLDTFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB6</accession>
    <name>T1GVB6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02053258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB6"/>
    <dbReference type="STRING" id="36166.T1GVB6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007707-RA">
        <property type="protein sequence ID" value="MESCA007707-PA"/>
        <property type="gene ID" value="MESCA007707"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_518064_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF21">
        <property type="entry name" value="DISCONTINUOUS ACTIN HEXAGON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="210"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="404"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007707-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="60587" checksum="3D23E0034DB364D3" modified="2013-10-16" version="1">MMTFLWRIFDPQRTHSIFLIEIKEVLLILCKVSSIQHLTSEMFALVCNQNRCISLPRLQLLLAIFIKFLVEIGEESAYGSHNIPEIMNQCHSLYPGVLGIDEYQFQCLWSGKQTRFLIYANLLALVKRMNDTEKLIHKTQCFGCHTERIIGIRFKCKKCTLKDDRCYCDSEISENDKLSYTLCPIRCSKNKSQYCGGVDAMSYYVMNGKRVGNFPKIINRTETSLTIEWDYIRRKHGITEYTIKADILKTYAKESFYSPPEFVVASDQDRYEISNLHPGTEYNITLTAKCGAEVCDSVSIKDETLIENTHIHVSLGLVSERFDIVRIRYGPASHDHDTVEDFQNFKFADFEEGQNSIIALSVACVILAYDAQSSSRTLDFRSELNKIIDNLDRSQRYPRNAIRLNVNTILADGEFGEVVSGTLYTDSGAIESQVHVISDDMEKKSQYTFLQDFQNLIRVMPHEKFVNFLGVSASPDWFYLLFENKHTSLKNFLLDSRNNLVLHEGVILQIIYDISSAMEYLEKIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVH6</accession>
    <name>T1GVH6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hexosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus</location>
            <location evidence="1 9">Golgi stack membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CAQQ02178288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVH6"/>
    <dbReference type="STRING" id="36166.T1GVH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007780-RA">
        <property type="protein sequence ID" value="MESCA007780-PA"/>
        <property type="gene ID" value="MESCA007780"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264374_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF13">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007780-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="25463" checksum="713C768207F34B7D" modified="2013-10-16" version="1">HNSAIIHPLTNVDDFYRLHVYFSKNHLNDLREKNRKLEHDTYKIANGSISNNILEIRWPLGVPEQGVAETRYDINVWQHLNKTHMFIPNAESAVEPLSRIDSMDFQKVLEVAISYARSKYPKIYYESIHTAYRRFDPTRGMDYHLHLNFRAKSQDTILKSFEIVKPLGRVEVVPSPYVTESTRIAVLLPSLTLIKLDSFGQTLLGEIYIANTPKYYVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW78</accession>
    <name>T1GW78_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW78"/>
    <dbReference type="STRING" id="36166.T1GW78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008050-RA">
        <property type="protein sequence ID" value="MESCA008050-PA"/>
        <property type="gene ID" value="MESCA008050"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170555_0_0_1"/>
    <dbReference type="OMA" id="VIIMRHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033181">
        <property type="term" value="C:plasma membrane proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008050-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9652" checksum="37ADC3EE20EC0D1B" modified="2013-10-16" version="1">MYPVYIVLPCFTLFWCLVGILGPIVTPRGPNQQIIRCMLMLTAASCYLMWFCVYMSQLNPLTGPKLSKNKYILMQWEWNRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWF0</accession>
    <name>T1GWF0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWF0"/>
    <dbReference type="STRING" id="36166.T1GWF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008124-RA">
        <property type="protein sequence ID" value="MESCA008124-PA"/>
        <property type="gene ID" value="MESCA008124"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830196_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16357" checksum="FF5AB12ADDDFC95D" modified="2013-10-16" version="1">MNTNVQGVRGRFSKFLFFSNIFHAAIERGDPAYIYDTRWETVARTTTGPMQEFTVSFQSLLPSTSYSFRVISYNKYGISYPVYSKDSILTPSKLYLEYGYLQHKPFYRQTWFMVSLAATSIVIIITIIAVLCVKSKSYKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWL4</accession>
    <name>T1GWL4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02076212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02076213">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWL4"/>
    <dbReference type="STRING" id="36166.T1GWL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008191-RA">
        <property type="protein sequence ID" value="MESCA008191-PA"/>
        <property type="gene ID" value="MESCA008191"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294839_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008191-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="10205" checksum="5CE83F099367BC73" modified="2013-10-16" version="1">MDNSTERALNDANRALVNIIGELKHIGWLSKRLGGSTSGSVGGGTGSGPGSASSSGGGGIVGITSSAGNGCDQSGRSSSESSEENDKWQSIFVAITEREFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWW2</accession>
    <name>T1GWW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195188">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02195189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008296-RA">
        <property type="protein sequence ID" value="MESCA008296-PA"/>
        <property type="gene ID" value="MESCA008296"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_291465_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000060">
        <property type="entry name" value="Acrosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577718" description="Peptidase S1 domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="39"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="306"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="555"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008296-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1048" mass="113820" checksum="DD184A0CDD6B44EC" modified="2013-10-16" version="1">MKTFAIVSFLLIGAAFADVVPAKLPEQFLKKLYNIQPRVVNGNDAIPKQFPYQVGISTRRGDAFYWCGGSVISESWVLTAAHCVVGDIHSLNLIFGATNRLDTYQVGQARQNISRFEEGQVIVHPLYNPSVILNDVALVRLPQKLEFSEYIQPIRLASSNSFQTFSNSPAVISGWGKVANNGSVVQHLQYGYVTILDLNACKSSYNPGLVTQGNICLDVKKSGVSSCGGDSGGPLVSXXXXXXXXXXXXXXXXXNCYWRIDWSYFFRIGCWLRTRALFLGAALADVIPAQLPQEFLRKMSNISPRVIGGSIAVEKQFPYQVGISTKKGGNFYWCGGSILSERWVLTAAHCVVGDVHSYLLIFGATDRLNPYQEGQSRQNISNIDDDKVIVHPLYNPAKITNDLALIRLPQKLEFSEHIKPVKLPSNKSYNTFANSPAVLSGWGKTANNGSVVQHLQYGYVTILDLNECMSRYNPGIVTQGNICLDVAKSGVSSCNGDSGGPLLSTVTGELIGVTSFGSAAGCEHGAPTGYSRVTYFRDWIKILTKLHKKVLQPRVVGGNYAAAKQFPYQVGISTQKGGGLYWCGGSVISEEFVLTAAHCVAGDIEALTLIFGATDRNNAVQTGSSRQKISHFKNGQIIIHPLYNPYEITNDVALVRLNQKLEFSEYIQPALLPEPGRFQTFANSPAVLSGWGKTEDSGSVVQYLQYGYVTILDLNECMAFYNTPEYNDANICLDVKNSGVSSCGGDSGGPLVSTTTGELIGVTSFGSSAGCEKGYPTGYSRSTMKVLAVLAVCFLSAALADNILPAELPKEFYAKLQTIQPRVTGGTNAVPKQFPYQVGVSTKKNGGFYWCGGSVISEEWVLTAAIALQDRLDAYQVGSSRQNITNVKGQIIVHPLYNPNEITNDVALVRVNQKIEFSDYIQPAKLPENNQFKTYTNSPAVLSGWGKVQDSGSVVQHLQYGYVTILDLNECMAYYSIENYTEGNICLNTKDSGISSCNGDSGGPLVSTATGELIGVTSFGSIFGCTGGAPTGYSRVTFFRDWIKYHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXP8</accession>
    <name>T1GXP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02375324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXP8"/>
    <dbReference type="STRING" id="36166.T1GXP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008602-RA">
        <property type="protein sequence ID" value="MESCA008602-PA"/>
        <property type="gene ID" value="MESCA008602"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099298_1_0_1"/>
    <dbReference type="OMA" id="NIRDFED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008602-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15748" checksum="41E1EA2C1FB40F71" modified="2013-10-16" version="1">MWPEDEGCDVLYDKFDFIVVGAGSAGSVVGARLAVNPQYKVLVIEAGESPPIESEVPGLLLTLQNTKYAYNYTTEHSDKYCLAFNNGSCIWPRGRMIGGTGSLNGMIYLRGNPGDYNDWNVEGWKYEDVLPYFEKSQQFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXR4</accession>
    <name>T1GXR4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calx-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02055327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GXR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008619-RA">
        <property type="protein sequence ID" value="MESCA008619-PA"/>
        <property type="gene ID" value="MESCA008619"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012872_0_1_1"/>
    <dbReference type="OMA" id="ETNANHF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF65">
        <property type="entry name" value="NA_CA-EXCHANGE PROTEIN, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588604" description="Calx-beta domain-containing protein" evidence="13">
        <location>
            <begin position="27"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="14">
        <location>
            <begin position="388"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="14">
        <location>
            <begin position="501"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008619-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="576" mass="64846" checksum="9401BC94C69BA154" modified="2013-10-16" version="1">MAGSLIFGKRKLFLVICIVLFGIITAENNDGDDVSNNTPNISHNIVKELGNMSYLHRPNDIEFEDEDDIKVGPHDKGRAVCQEGLVLPVWMPQENLTVGDRICRGLVYFLAMIYLFIGVSIVSDRFMAAIEVITAGDLGPGTIVGSAAYNLFVIIALCIFVIPNGEVRKIKHLRVFFVTATWSIFAYIWLYLLLAVISEGEVEVWEGVVTFMFFPATVLTAYIADRRLLIYKYLDKNYRMNKRGMIVASERDATLEMNGGDELKGLEDQSMNDEMKDFXXXATLEMNGGDELKGLEDQSMNDEMKDFEEARREYISTLKDLRKKYPQSDLEQLEMMAHEMLINKGPKSRAFYRVQATRKIIGSGNIMRKIQERAQSDLSEIKAELHRVDTDAQEDDTIPRIFFEPGHYTVMESCGDFEIRVVRRGDLSTHVTVDFETEDGSAEAGADYIGKKGTLSFPPGVDEQKIRIEVIDDDVFEQDEHFYVRLSNVSDPAILATPKVATVMILDDDHGGIFAFASADHELIESVGVYELKVQRFSGARGKVAIQYWTEDGTAKAGKDYEAQSGELVFENNETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXV7</accession>
    <name>T1GXV7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02028902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXV7"/>
    <dbReference type="STRING" id="36166.T1GXV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008668-RA">
        <property type="protein sequence ID" value="MESCA008668-PA"/>
        <property type="gene ID" value="MESCA008668"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021695_1_1_1"/>
    <dbReference type="OMA" id="SHAICIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.519.10:FF:000005">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF8">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008668-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17023" checksum="9F1CE11FCC46E522" modified="2013-10-16" version="1">VRIKGKVVRCICILDHPIPNTKDALSTQIIIPQKQVGRKSDIYVSLVSSTHQVAAKGFFIAMVSTTVESENPEAEIKPGLDLLGPIAQKFVSVSDYYEPTDKGLESQIFISESYDATTHFETTCLDVLDIFKRATGEDFDFSKIKHEIGDEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXX1</accession>
    <name>T1GXX1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02169153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXX1"/>
    <dbReference type="STRING" id="36166.T1GXX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008684-RA">
        <property type="protein sequence ID" value="MESCA008684-PA"/>
        <property type="gene ID" value="MESCA008684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124087_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="2-oxoglutarate dehydrogenase E1 component N-terminal" evidence="9">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="20677" checksum="AF4143BD114F4C84" modified="2013-10-16" version="1">MASPKKIATILFNGHKTRNLLPVLSAKSYNSAASEPFLNGSTSNYVEEMYNNWLRDPTSVHTSWDAYFRSNTYAAPPNLAPTMRNMVPLSAVSGSAMAVGQPDEKIIDDHLAVQAIIRSYQSRGHLAAELDPLGIINPESSISKDGVARRANEAVLRQHSWFMMGNLFLHILLKAITIFVKFGVISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXX3</accession>
    <name>T1GXX3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXX3"/>
    <dbReference type="STRING" id="36166.T1GXX3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008686-RA">
        <property type="protein sequence ID" value="MESCA008686-PA"/>
        <property type="gene ID" value="MESCA008686"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_761385_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008686-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41700" checksum="2BDA8EA952D73B6F" modified="2013-10-16" version="1">MQCMRYENEVDKRFKGSSKISEIDDRFPSASSNTPCVAPNDQFSSVDEIKYPKERPKIQCSNCHEPREWESPNTQLSLHLNSNLTDVSPIHVLQRPLESKSVVPSVPKLSNPPSLHERKVRYEQRRKEIFANVDSIAKVDLPKNSVVGVRQRFRLWRTVSKNIAETLLDKPSDMRKYALVKINGKSIEGLLDSGASITCLGRGSHNFLESLGLSITLRKSKVKTANGTSAEIIGSFKAEVSFKGFTRELLIFVAPNLTQNLYLGVDFWNSFKLWPSEISENLEKSHKRYEKCYNLRSRPVKFHQGQEVFKRNVILSDKNKHINAKLCPKFMKCKVRNVLAENRYELETEKGKFLGIYHAQDIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYE0</accession>
    <name>T1GYE0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008865-RA">
        <property type="protein sequence ID" value="MESCA008865-PA"/>
        <property type="gene ID" value="MESCA008865"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_751376_0_0_1"/>
    <dbReference type="OMA" id="SAERICW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038877">
        <property type="entry name" value="THSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16311">
        <property type="entry name" value="THROMBOSPONDIN TYPE I DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16311:SF3">
        <property type="entry name" value="THROMBOSPONDIN TYPE-1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008865-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="369" mass="41871" checksum="F447E27F1676ECCA" modified="2013-10-16" version="1">MKLIWGFTCPITINTNANTTRSGSTSSEEIIIPSDRRHSSSNTPAHLLISRLENNKKDLINRIKVYPDIRNNVTQIHIPCRNFQRGGVYELQIVEDGLEADSQTLDINDERLKQQLDVRWPVPELKVSPQKFGTYPENPVKVLVSFPDVECELTRATALKANISEKQLPEFWLELIYCGEENLCDGSNSSINSISGTNTKSSVLYAEQILGFPQERRITLKCELFGFAGHYAIVLRPTKPTPSFVTAISYVQADWSKKFVFNVHARSIFPCDPHTGVGVLFEYPACILDKSDRVRLYAKLRADVASLKPPTSLHYVTEQRVNKGKHSLHFDCDLFSEKYVEYCFVYVSQAITDAGPTFEWTVFQRSPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYT4</accession>
    <name>T1GYT4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYT4"/>
    <dbReference type="STRING" id="36166.T1GYT4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009021-RA">
        <property type="protein sequence ID" value="MESCA009021-PA"/>
        <property type="gene ID" value="MESCA009021"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197445_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009021-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="232" mass="26145" checksum="0B8344169F0A8319" modified="2013-10-16" version="1">QNKTFSTVCLCCASILLPSIDERNSKGKAISLNFHLRYQNRTRIERKLHPKIRTGEFLESKKMVVAMLKYPLILLFLSIPTSMLLWLGNVGFSKISNSPDHDEDGVIPPTRWLFSILCPMMLMLYALKRKSVCASGAFLGIIVAFILSIANHAFFACLVAFFFSSSRATKFRGHIKKKIEKDFKEGEGQRNWIQVFCNSGVATLLAILYLIDCGSGEKPINFKISTDLPGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ27</accession>
    <name>T1GZ27_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02194592">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194593">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ27"/>
    <dbReference type="STRING" id="36166.T1GZ27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009118-RA">
        <property type="protein sequence ID" value="MESCA009118-PA"/>
        <property type="gene ID" value="MESCA009118"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118074_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF313">
        <property type="entry name" value="PEPTIDASE M12B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="146"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009118-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="249" mass="28196" checksum="753325B0DA2EA2AE" modified="2013-10-16" version="1">FYKDGDWSPCSVTCGEGIRRKSYRCKIFLEYSRTIATVNDTLCEGQKPQDEVERCVEDPCMLPSHGYDEQYPRDSIKVGVSEPGKTYVWQEQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYSPCSKSCGIGIKTREVQCIHEVTRGVKIQWLYQIDHRPLARTKKFKVSRKGALRILDIKFPDAGVYSCHAGLSTAEINIDVKAKPGMQPEDYEKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZI9</accession>
    <name>T1GZI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02375250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009290-RA">
        <property type="protein sequence ID" value="MESCA009290-PA"/>
        <property type="gene ID" value="MESCA009290"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870140_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009290-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7358" checksum="21184C09A666CF20" modified="2013-10-16" version="1">MRYPLHQDQRCNNRAISRTSAKYLERPKSWADDCGWELIPAKLSLFSFPGNKLQFSGNRNSGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0R8</accession>
    <name>T1H0R8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C39 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0R8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009754-RA">
        <property type="protein sequence ID" value="MESCA009754-PA"/>
        <property type="gene ID" value="MESCA009754"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064395_0_0_1"/>
    <dbReference type="OMA" id="NSTWTID"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018616">
        <property type="entry name" value="GUCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31400">
        <property type="entry name" value="GUANYLYL CYCLASE DOMAIN CONTAINING PROTEIN 1 GUCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31400:SF1">
        <property type="entry name" value="PROTEIN GUCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09778">
        <property type="entry name" value="Guanylate_cyc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009754-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18658" checksum="4D9E93170ACD38CC" modified="2013-10-16" version="1">MDGLPCHKEYNLTYSPQITNTDCGLTCVAMLLEPSSKQYLIDNLEEIRNAAGFGNSTWTIDLCYVLRRFNIKFVYFTKTIGVDESRISDPNYEKSCAKDKSRVKRRFMEAESNGISIDKRTVELSAIVRHLANDGPLILLINDSTLECGICRKNKVKDNVKVGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H264</accession>
    <name>T1H264_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H264"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010297-RA">
        <property type="protein sequence ID" value="MESCA010297-PA"/>
        <property type="gene ID" value="MESCA010297"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870143_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7285" checksum="968DDAE7B95AA3D7" modified="2013-10-16" version="1">MSTNITNVVQQFHIHGSRVGKLFLCKAIQNGCKILSSKVRKITKARIQMSFPQSSGHFLWHMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H280</accession>
    <name>T1H280_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02087899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H280"/>
    <dbReference type="STRING" id="36166.T1H280"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010316-RA">
        <property type="protein sequence ID" value="MESCA010316-PA"/>
        <property type="gene ID" value="MESCA010316"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152072_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF37">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010316-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13157" checksum="68D2D1D8AAFC5569" modified="2013-10-16" version="1">QYKPWRLFILVCGLPGFFSALCLFKFPESPKFTLSVGREAYTLKSLRTIYTWNTGKDPAEFEIKSLIQDEGEQRESCSYSKLQIFRSMWNQTAPFFRYICCHKESSFGSHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H285</accession>
    <name>T1H285_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H285"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010322-RA">
        <property type="protein sequence ID" value="MESCA010322-PA"/>
        <property type="gene ID" value="MESCA010322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103919_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="261" mass="30475" checksum="2F10E58B6996BE45" modified="2013-10-16" version="1">MDLDVDNQEYNEAVSVKDKQFEELEFMRDRNQAWKFYQNPMPNNLYYLVNHTISENENRIRFHEIQVNLTQNRFDHYVETVRHNTEMVERTISEFTSELTLFGFLNETNQNCVVKYARIPDVEAFTNKINICANTASSTFNSYNSVLQVALNNLANAKTYNGNIRWESQVCYNNYIQYNSEKMPSCIQSSIDLRNASIDTALLNVQNELAKALNTYDFVSRDTTECIYKVEFELGKLIDNQWDVLRTCQKIGKVSTFFGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2T5</accession>
    <name>T1H2T5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2T5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010540-RA">
        <property type="protein sequence ID" value="MESCA010540-PA"/>
        <property type="gene ID" value="MESCA010540"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3074721_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="53" mass="6376" checksum="0377C4670DC97F64" modified="2013-10-16" version="1">MAKSPKRIVGKIRTVQFTEKTRKFNYVKLWLMVQTILVHIPIRTYLQICIFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2W6</accession>
    <name>T1H2W6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Plexin cytoplasmic RasGAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2W6"/>
    <dbReference type="STRING" id="36166.T1H2W6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010572-RA">
        <property type="protein sequence ID" value="MESCA010572-PA"/>
        <property type="gene ID" value="MESCA010572"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004205_2_0_1"/>
    <dbReference type="OMA" id="MAYQART"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF70">
        <property type="entry name" value="PLEXIN A, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plexin cytoplasmic RasGAP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010572-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13913" checksum="1E813C56F5FD8B0E" modified="2013-10-16" version="1">FWVNLIKNPNFVFDIHKSNIVDSCLSVVAQTFMDSCSTSDHRLGKDSPSSKLLYAKDIPAYRDWVERYYRDIREMSSISDQDMNTMLAEESRLHTTEFNTNCALHELYLYAVKYKNNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Y6</accession>
    <name>T1H2Y6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Y6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010592-RA">
        <property type="protein sequence ID" value="MESCA010592-PA"/>
        <property type="gene ID" value="MESCA010592"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190180_0_0_1"/>
    <dbReference type="OMA" id="FAMCCFA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054721">
        <property type="entry name" value="GEO12453p1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22861">
        <property type="entry name" value="GEO12453p1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577839" evidence="1">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010592-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="7585" checksum="32E34B5D5E3FF5D0" modified="2013-10-16" version="1">MFKFFAICFLALVAIVAAKPGIVAPVAYSAYSAPVVSAYSAPLVASTYSSPYVAASPYVASAYSAYPGAYFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H346</accession>
    <name>T1H346_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H346"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010660-RA">
        <property type="protein sequence ID" value="MESCA010660-PA"/>
        <property type="gene ID" value="MESCA010660"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742928_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010660-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8057" checksum="B753BDB5FAD9856E" modified="2013-10-16" version="1">MILDYTPNNTHPCYKASIILRPLRHQEKPIILKLQLPKPDASDVESDWRMCADLLTSTALVLGPEKRSTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3D7</accession>
    <name>T1H3D7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3D7"/>
    <dbReference type="STRING" id="36166.T1H3D7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010762-RA">
        <property type="protein sequence ID" value="MESCA010762-PA"/>
        <property type="gene ID" value="MESCA010762"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138927_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032673">
        <property type="entry name" value="DNA_photolyase_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052219">
        <property type="entry name" value="Photolyase_Class-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211:SF0">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTO-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01083">
        <property type="entry name" value="DNA_PHOTOLYASES_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010762-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="19256" checksum="8D7A4EDDFCBFD449" modified="2013-10-16" version="1">FPPVILHPFECKGNCARIPWKTILKQFTFNKQVKPVKWANPGYESACRMLYNFCETRLEYFADCRNNPPENYVSNLSPWFHFGHISCQRAILYIKQFQSKFPHSVDVFCEEAIVRRELADNFCFYNENYDNLSGISCKKWFNIPDFVKIYGGKVYEKDFVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4A6</accession>
    <name>T1H4A6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02106850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4A6"/>
    <dbReference type="STRING" id="36166.T1H4A6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011114-RA">
        <property type="protein sequence ID" value="MESCA011114-PA"/>
        <property type="gene ID" value="MESCA011114"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1059943_0_0_1"/>
    <dbReference type="OMA" id="WPINGVE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052448">
        <property type="entry name" value="DnaJ_C16_autophagy_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011114-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="263" mass="30661" checksum="B18CAE73890A3F53" modified="2013-10-16" version="1">QGILGVFWNKVLFLTEYLYDNVQHEHFLAAISIVGTIASILIVGYIMTHLVKIEEEKLIKNGSINQKTKISSCNQVQELKLHELRVEKYNGLVRLLKPGCRTIILITDLNSRKKLIPDFHKAAWPYRKNKTLMFGHMLIEKGLSWYSELLRLSLSDSRTLQINPRNCIGTVIALNGHRKYFCVYHAKHPDSRMDNKRILKMTKYLVKDFDEEEGLPSWPINGVENNKISEENLLAGLSNWLDRLFEGMTDRYYINYWPDFSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5L4</accession>
    <name>T1H5L4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02114978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5L4"/>
    <dbReference type="STRING" id="36166.T1H5L4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011595-RA">
        <property type="protein sequence ID" value="MESCA011595-PA"/>
        <property type="gene ID" value="MESCA011595"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2549517_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000748">
        <property type="entry name" value="PR domain zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000833">
        <property type="entry name" value="PR domain zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011595-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9565" checksum="A90809C38191CF70" modified="2013-10-16" version="1">MRYMQEKKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCKGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Z6</accession>
    <name>T1H5Z6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="9">dUTPase</shortName>
            <ecNumber evidence="9">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="T1H5Z6"/>
    <dbReference type="STRING" id="36166.T1H5Z6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012129-RA">
        <property type="protein sequence ID" value="MESCA012129-PA"/>
        <property type="gene ID" value="MESCA012129"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068508_2_1_1"/>
    <dbReference type="OMA" id="RSGMGHK"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000004">
        <property type="entry name" value="Deoxyuridine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="11">
        <location>
            <begin position="29"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012129-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="166" mass="18263" checksum="07025A9F9BE9EA2D" modified="2013-10-16" version="1">SEQEPAPKKMRTDTKPILRFAKLTEHAFAPMRGSAKSAGYDLRSAYDIIVPARGKAVVKTDIQIHVPEGSYGRVAPRSGLAVKNFIDVGAGVVDEDYRGNLGVVLFNHSDTDFEVKKGDRIAQLICERIFYPELVEEKSLEETERGEAGFGSTGLQEIPEKNGKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6E9</accession>
    <name>T1H6E9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6E9"/>
    <dbReference type="STRING" id="36166.T1H6E9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012282-RA">
        <property type="protein sequence ID" value="MESCA012282-PA"/>
        <property type="gene ID" value="MESCA012282"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3034726_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012282-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="55" mass="6292" checksum="420E3D42A0A9F1DA" modified="2013-10-16" version="1">MKNKKNSQRQSNQQNNNNSSSSNHNHGQPPQPHHNPHAHLNLNMGLSHHTSKMHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6I2</accession>
    <name>T1H6I2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6I2"/>
    <dbReference type="STRING" id="36166.T1H6I2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012315-RA">
        <property type="protein sequence ID" value="MESCA012315-PA"/>
        <property type="gene ID" value="MESCA012315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167752_2_0_1"/>
    <dbReference type="OMA" id="KPDQKEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.820:FF:000001">
        <property type="entry name" value="Transport protein Sec61 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7626" checksum="7382238A36A95687" modified="2013-10-16" version="1">MDKVMKVCEPGRAFAKDSIRLVKRCTKPDRKEFQKIAIATAIGFCIMGFIGFFVKLIHIPINNIIVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6P6</accession>
    <name>T1H6P6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6P6"/>
    <dbReference type="STRING" id="36166.T1H6P6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012379-RA">
        <property type="protein sequence ID" value="MESCA012379-PA"/>
        <property type="gene ID" value="MESCA012379"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2139705_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012379-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12709" checksum="4E4500E29A346CD2" modified="2013-10-16" version="1">PEANSWLKDAQDSCCPNLTRLQRIIGFVLCLGMGVFCFSLSIFFIPVLVLKARKFAMLYTLGSIFFITSFCFLSGFVAFFKQFFSKQRLAVSLTYSSSMMATLYFSLIAQSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAA3</accession>
    <name>T1GAA3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRAL/TRIO N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAA3"/>
    <dbReference type="STRING" id="36166.T1GAA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000153-RA">
        <property type="protein sequence ID" value="MESCA000153-PA"/>
        <property type="gene ID" value="MESCA000153"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1706295_0_0_1"/>
    <dbReference type="OMA" id="YANWNVE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000153-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18161" checksum="F16BC5289D98589F" modified="2013-10-16" version="1">MLLFDEGNFDFCAEEIEKIQLLRKLVEASPELQIGTEDTYLSRWLYYANWNVEGAFIAIDKYFEFKAHNPSWFNNLKNIDELKDLIINVKTRYVLKKRDVHGRIVIVQLLNDAFDKYSDYFFDLVRLDDIIFEALLSTANVQKYGISIINDFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAA8</accession>
    <name>T1GAA8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176808">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAA8"/>
    <dbReference type="STRING" id="36166.T1GAA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000160-RA">
        <property type="protein sequence ID" value="MESCA000160-PA"/>
        <property type="gene ID" value="MESCA000160"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877786_0_0_1"/>
    <dbReference type="OMA" id="FVWVWLE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000160-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="14598" checksum="066E95FA2EF29927" modified="2013-10-16" version="1">MRVRTSEVLSYNVSAWQESGRTGAVSRKTSQNPTPTDTDSGPSDRATPASKNDSESKEETSSNGEKREKSNSDLTDQPKPPSAGASIRDSANKILGLAVKGEWTPVEQEMKKLEKYVANAGEDGNHVPLLGVHDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAD5</accession>
    <name>T1GAD5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000190-RA">
        <property type="protein sequence ID" value="MESCA000190-PA"/>
        <property type="gene ID" value="MESCA000190"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349063_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000190-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11287" checksum="CA746E78D9C283EA" modified="2013-10-16" version="1">MEPMDLILNNPGKSDQNLVSDTTIRICNNVQNHYGILRLKKQRKLPESVLLISSTSKFSRLIVISEMDKRDVRQELERKYRGNQKKDYAVSRQILGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAR5</accession>
    <name>T1GAR5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAR5"/>
    <dbReference type="STRING" id="36166.T1GAR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000325-RA">
        <property type="protein sequence ID" value="MESCA000325-PA"/>
        <property type="gene ID" value="MESCA000325"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639620_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000325-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18618" checksum="8A7DB981B5618511" modified="2013-10-16" version="1">MFWTNTKIYLNDIVIEKRNEAIDSLRTSENIPLLLSSQKEFIKNIAECVYLGKPVLLVGSSDSGKTKLIDSYCFLSGQICNNDIIDDSVTGTFQQIRADFNYNGPDFTERMLSILFSENGKEKEIPLNCLHLWKSKVCEIYEVCWSNLYLQHSDYDISMASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBT2</accession>
    <name>T1GBT2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBT2"/>
    <dbReference type="STRING" id="36166.T1GBT2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000720-RA">
        <property type="protein sequence ID" value="MESCA000720-PA"/>
        <property type="gene ID" value="MESCA000720"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072084_0_0_1"/>
    <dbReference type="OMA" id="HHSIRNG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF3">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="83"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000720-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="25223" checksum="BF54E320F47AF320" modified="2013-10-16" version="1">MIFSKAINNYRIRKIENIGKMTKMTQDEIISNTRTVIQGLEALRIEHHNLISGTSDNESNEKSHIIKKNIESLELGLGEAQVMMALASHLQNIEAEKQKLKTQVRRLYQENAWLRDELANTQQKFQTSEQTVAQLEEEKNHLLFMASMKKIEDENQDPEENSEKSRPDPVVELFPDEDHDDRNMSPTPPSHFANQTSGYEIPARLRTLHNLVIQYASQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBZ3</accession>
    <name>T1GBZ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389373">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000786-RA">
        <property type="protein sequence ID" value="MESCA000786-PA"/>
        <property type="gene ID" value="MESCA000786"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888310_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000786-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7181" checksum="CC38EA8A7A1B4BA2" modified="2013-10-16" version="1">MFPNVSPIKLELFIWNSLCLKFNSESAKLKLTPNQLTNSKILIFLKASKGVILSGLMRKRPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCJ0</accession>
    <name>T1GCJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gem-associated protein 5 second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCJ0"/>
    <dbReference type="STRING" id="36166.T1GCJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001004-RA">
        <property type="protein sequence ID" value="MESCA001004-PA"/>
        <property type="gene ID" value="MESCA001004"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_636628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056424">
        <property type="entry name" value="Beta-prop_GEMI5_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051510">
        <property type="entry name" value="SKI8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090:SF1">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23775">
        <property type="entry name" value="Beta-prop_RIG_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Gem-associated protein 5 second beta-propeller" evidence="5">
        <location>
            <begin position="90"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001004-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="431" mass="48698" checksum="40152DDC6779BC7B" modified="2013-10-16" version="1">MSKSKAKVFIENVELRKIVEEQTWPNKPEEEQFINASSITRRKQFAKRKRSNDATKKTSPSASVEVVKVSASCSTSTGRVEETTLQDVRNGIKLPQIKEASSVTVRNSMLIVGTQSGTIEYYKLKSKDNPEDIELVNKIFVSPKYISDINWSSIQLNRFAVCSYSNKIAVLRITAEDIMKWKVPESANCKNVCVKFSNRNENMLLSCGSDGGIRIWDLKQSPTKELIYVKWFTSPMNSALFSPIDENVVICCGKSVSIELFDMSKEPSDNILESKVSKTKSLNIQWAVTVLTAESTGNAKSRRNRKKVNGIPKKDEDEEDISEKLDDLKLEPKTSPLKTRHGFMKSEVTVLYLSHKETNRQDLQSLNLFLNNNSETHPLMSTKLFGKSCSFFLGSMSVKQNRFPFNGEMGPIMLMLMYSHLELGICCVTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD50</accession>
    <name>T1GD50_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001231-RA">
        <property type="protein sequence ID" value="MESCA001231-PA"/>
        <property type="gene ID" value="MESCA001231"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1134129_0_0_1"/>
    <dbReference type="OMA" id="VVTRKKW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040044">
        <property type="entry name" value="SRR1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626:SF3">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="64"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001231-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="239" mass="27791" checksum="E97B4A5A795CF3E8" modified="2013-10-16" version="1">MDHCKQEKERKTKIIKENLSNVVPGEDDHDFDLENFKKRLESIRKELSSSKFFENLIEKLISLPKSYENILCLGLGRISSCHIALHQLSLLLELKTALRTNSVTVFDPVFSKQEKEILNSLECKVPEENMEGKYNISEGTLAYFPHCPHELTDAFLSENFTKEKLLKIVLLCNSFERFTTSEPSRIIEERAPFIGKIVDFSNIFEIENNYKFEDVFNDTAIHSFLEDKLPEEGTDFWDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDD6</accession>
    <name>T1GDD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02125765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDD6"/>
    <dbReference type="STRING" id="36166.T1GDD6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001321-RA">
        <property type="protein sequence ID" value="MESCA001321-PA"/>
        <property type="gene ID" value="MESCA001321"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901478_0_0_1"/>
    <dbReference type="OMA" id="AWAPHRK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001321-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="14912" checksum="C487BD1B8B250FC6" modified="2013-10-16" version="1">QPHGRVYVAGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYEDPDRTALLRLAWNKQDPNYLATVAMDSCEVIILDVRVPCTPVARLSNHRACVNGIAWAPHRKCPVVTTNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDP3</accession>
    <name>T1GDP3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001434-RA">
        <property type="protein sequence ID" value="MESCA001434-PA"/>
        <property type="gene ID" value="MESCA001434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429573_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10669" checksum="014EB5E8E870616B" modified="2013-10-16" version="1">MLNTLETFNKVMLRAKQGSSRHILWSVKVSVPLGVGREYFSSKLRDNCNRRWKLLDSFEVTMKTWCRFDERASNSLITLGRNSLRKIVNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEE9</accession>
    <name>T1GEE9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02179291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEE9"/>
    <dbReference type="STRING" id="36166.T1GEE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001711-RA">
        <property type="protein sequence ID" value="MESCA001711-PA"/>
        <property type="gene ID" value="MESCA001711"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062797_0_0_1"/>
    <dbReference type="OMA" id="DIRSQPH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29059" checksum="4E0BCFD748F26CEA" modified="2013-10-16" version="1">MSTVATPLSIAGTRRYGQPVRTQNVMAAASIANIIKSTWVKKRDLKIIAKAKGAAFLTSLTNMDGEEGFNASIVGEAAQERICDDELILIKGPKVRAAASIILRGSNDFYCDEMDRSIHDALRWMCGSCSFNLLGTLCYLFKFKRTVAEFAKSLLVIPKLAVNAAKDATDLVSKLRVYYNSSQTKSEHADMKWVGLDLFKGVVRDNKKTGVLEPEMSKIKSLKFATEIAITVLRIDDMIKLHPKTRAENGSPCFGRDYISHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEI1</accession>
    <name>T1GEI1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEI1"/>
    <dbReference type="STRING" id="36166.T1GEI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001745-RA">
        <property type="protein sequence ID" value="MESCA001745-PA"/>
        <property type="gene ID" value="MESCA001745"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_1_1"/>
    <dbReference type="OMA" id="YSDWISE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000073">
        <property type="entry name" value="Trypsin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577311" description="Peptidase S1 domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="32"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001745-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="26260" checksum="4635AC36CD6A18C1" modified="2013-10-16" version="1">MIRFVLVSTLIACALAALPKIPKRHYGPGGKVVNGFDLDIEEAPYQVSIQYNGGPHFCGGSLISPDVVLTAAHCVEDDPRPGTIIIYAGSSDRLVGGQRRAAKNIIYHENYDPKEIFNDVALIRLRRPFEYSDKIQPATLSSSDPAPGSIGLASAPRHLQGVELDIISLEDCRRAYGTAAIQDSNLCAYTPDKDTCQGDSGGPLDVDGILVGVVSWGAGCAQRGAPGVYASVAAYSDWISETIKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEP0</accession>
    <name>T1GEP0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001810-RA">
        <property type="protein sequence ID" value="MESCA001810-PA"/>
        <property type="gene ID" value="MESCA001810"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796904_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001810-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7931" checksum="173F971356F7DC13" modified="2013-10-16" version="1">MNHSRAELEDITANKTNDTNFVIPQSTFSELRSRKKWISHKQINPHQIIAKSSSKIPNQQPNELLYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEQ9</accession>
    <name>T1GEQ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02078989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEQ9"/>
    <dbReference type="STRING" id="36166.T1GEQ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001830-RA">
        <property type="protein sequence ID" value="MESCA001830-PA"/>
        <property type="gene ID" value="MESCA001830"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_687514_0_0_1"/>
    <dbReference type="OMA" id="WWKETKG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF158">
        <property type="entry name" value="V-SET AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN 10-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="12"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="104"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="215"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001830-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="401" mass="44228" checksum="C9D7DF99E34EE5F6" modified="2013-10-16" version="1">MDSYASAASFGMQMVKSPESSVAPEGDEVIFECEFNLAPDKFIWRFQSEQNKDNEFKYLQKNGDGYSISNHEASSKLRIYVRPSNIGKYQCVAWYGDSAIVSLPANLTLASIQEDKSYANHKSVWKISPGNTVVIRCGSVTSNPSAVWSFYKNGEKLKNTDNLFRADSLVLNSVSHLDSGSYSCEAINSITRQQLRISHTIDLRIDSSNSSTFFPLTPSSIVRAIEGQSAILDCAGVGTPPPETIWVNQNKPNFLNKDVKILPIGLYFSNVTKTNAGIYECILNNGRVPTIKKTITLEVLTKPEIIKGISENEISAKEEQPETLSAKQLGSLNQEFDGIIQCFAENEVGQVFQANFFKVIPIVRNNGPITSSHRPTPGSKYTPPTTPIMNYGLREIGASVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFV9</accession>
    <name>T1GFV9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02040690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFV9"/>
    <dbReference type="STRING" id="36166.T1GFV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002261-RA">
        <property type="protein sequence ID" value="MESCA002261-PA"/>
        <property type="gene ID" value="MESCA002261"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_450021_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000056">
        <property type="entry name" value="NADPH oxidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF58">
        <property type="entry name" value="NADPH OXIDASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="271"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="541"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="607" mass="70481" checksum="EC2160276D78997B" modified="2013-10-16" version="1">MEFSEEQIEDLTMAMFDDADPQHKGSLTYEALKDQLQKHGGLLENLSITIDRWLVPQSKSQEDKNRKQFKFQIPHQLTKAYLKNNQKYSISPSNGYTILARACGQCLNFNCAWVLVLMLRHSLTFLRSRGFSSYLPLDHHIYLHKVTGTVIAVLSLVHTIMHLINFSVVVVNDPVLNAQNYTVCQWLFTSTPNLFGLVSGWANPTGFALFFILLVMFVCSQPFIRRKGSFEIFYWTHLLYIPFWILVILHGPNFWKWFIIPGLIYLIERIMRFVWMKSKRGKTYISSGLLLPSKVTHLVIKRPPNFTFRPGDYAFINIPNIATYEWHPFTISSCPEEEDYIWLHIRSVGEWTNRLYMYFEKEHEKLHNGEIEPYISDIRSEKSESKTPQLDFLAKNMSNIKRSDHNDLPPPMRPPRQGHGPSKLAIENATITQTMKKDARKDSGYIDEFHMNGKNKNKTPLEKSLSMPNIANRTHRRLRVEYNRSESAKSFDENKIRRAHVQNLGLAYLSPQNKSLAQSFRYMRNKPTIIAFKTPSLENCEKRDSSNSMASTVYSPKEAEEGKVRKSSNNRGVNYPVGKPLKLLNPLFCPEKIKIFLIMSRNNPLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGB3</accession>
    <name>T1GGB3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ada2b tri-helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02142044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGB3"/>
    <dbReference type="STRING" id="36166.T1GGB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002427-RA">
        <property type="protein sequence ID" value="MESCA002427-PA"/>
        <property type="gene ID" value="MESCA002427"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222311_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056267">
        <property type="entry name" value="Ada2b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24533">
        <property type="entry name" value="Tri-helical_Ada2b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ada2b tri-helical" evidence="3">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002427-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="25862" checksum="7C7B4087A2EFB295" modified="2013-10-16" version="1">MVNEQQVLYDNASQEINKLGNQVDTFSPFQHKVKMEEPDVKRENSSSIEPKHMLPIGDRTYSDGLSPSKNFSDNYRGLSGHQMIVLSPTTTTQLTAVNNKFPANSTTPVEAATTTSLPLITLIQNSNGNIKLGEDEKRHILQQRLARKQVQLQQQHHQQQHHQHPQQQTNEVLTNDIISIFLKNQLTLKQQQIPQNEENLLTHNEIKLCSKLNILPSEYISMKTVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGH3</accession>
    <name>T1GGH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02010144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02010145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02010146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGH3"/>
    <dbReference type="STRING" id="36166.T1GGH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002493-RA">
        <property type="protein sequence ID" value="MESCA002493-PA"/>
        <property type="gene ID" value="MESCA002493"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1572425_0_0_1"/>
    <dbReference type="OMA" id="CWITRRS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="9">
        <location>
            <begin position="2"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002493-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="19388" checksum="C7978C0E3955A881" modified="2013-10-16" version="1">MDIFNEIRSGAYRKLFNPNMLITGKEDSGSNYSRGYFTLGSEMIDYTMNNIRLLAENCNNLQGFMIFRSFGGGTGSGFGNLILEKLLDDYGRKSKVEIDIFPSPKMLKKFGTYQIAFIWHSLMSFLGMPNCFIALNSINLHNMLWFHFFQKLVPCPSITPNDKESLLAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH99</accession>
    <name>T1GH99_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002789-RA">
        <property type="protein sequence ID" value="MESCA002789-PA"/>
        <property type="gene ID" value="MESCA002789"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485911_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10190" checksum="ADE9599C58C290F4" modified="2013-10-16" version="1">MLKFFTCASHFDLFSLPSLWYVRSVISLILYSVLELFGYGPLLGPASLEELSFIGEYIFRSPYTNKEFSIMKIEKLMESNWPSWHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHG0</accession>
    <name>T1GHG0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHG0"/>
    <dbReference type="STRING" id="36166.T1GHG0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002857-RA">
        <property type="protein sequence ID" value="MESCA002857-PA"/>
        <property type="gene ID" value="MESCA002857"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF134">
        <property type="entry name" value="AT07769P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002857-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="6847" checksum="44969CC755F4C43D" modified="2013-10-16" version="1">DNICVATGGKSICSGDSGGPLVTLDTYEQIGINSFVSGGGCEGDAPAVLVRVTNFLDWIKENTGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIP9</accession>
    <name>T1GIP9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIP9"/>
    <dbReference type="STRING" id="36166.T1GIP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003333-RA">
        <property type="protein sequence ID" value="MESCA003333-PA"/>
        <property type="gene ID" value="MESCA003333"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064864_1_0_1"/>
    <dbReference type="OMA" id="NEWINHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15717">
        <property type="entry name" value="FYVE_PKHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051765">
        <property type="entry name" value="PH_domain-containing_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280:SF3">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="89"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003333-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25056" checksum="3E0BF58FF87B0AFB" modified="2013-10-16" version="1">YGNIVIGKKKYNKQHIIPLEEVQLESLEDSDQYRNGWLIRTTTKSFVVYAATSTEKQEWMAHINKCVEDLLRKSGKKPVENHAAVWVPDSEAQNCMLCKKSQFTMINRRHHCRNCGSVVCGPCSSKKFLLPQQSSKPVRVCLDCYDKLNKAKSDQKSNNNTTTTTTTNPNDSSGDDDSDDGEEGDNNRSQVQYDEAKFYGDTSRAVNSSLNESAGNATNQEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ52</accession>
    <name>T1GJ52_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003494-RA">
        <property type="protein sequence ID" value="MESCA003494-PA"/>
        <property type="gene ID" value="MESCA003494"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1196030_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003494-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26685" checksum="E3739298116DE1DC" modified="2013-10-16" version="1">MKLVFLLVFQYRRFARLSNRLLKVPFKGTNKFTLNELIAYFLIFTTVLINLGVGIYDIVMLRRYSMPFVNFMLFSALYLPHVALAGCVKYLNTLIWMLTSFFAEIREEIDKSTDRILNVDENGTTVITLTDSCGDLKKSRVTFVEKLDALIIIVNKLNERVQKQLYSLIGLNVICLVFAAYSLVYYNTTWHLITSEAFGSALRVTCILCLSAFWSTIFPWHSATVVLKRKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ93</accession>
    <name>T1GJ93_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA003537-RA">
        <property type="protein sequence ID" value="MESCA003537-PA"/>
        <property type="gene ID" value="MESCA003537"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097305_0_0_1"/>
    <dbReference type="OMA" id="YRKHITR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003537-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="29406" checksum="A424ADFC2CFFB3CF" modified="2013-10-16" version="1">TSEQLQKLIQAVQTSSSGAVAPSQSKGSFGNCEARFNGDRDHHVVEEFIINVTTYKEIEKVSDEDALRGLPLLFCGLASTWWQGVRKEATSFVHAMEMIRSHFSPTKPSFQVYQEIFQEKQGDGTVIDTFICEKRALLAQLPDNRHDEETQLDFVYGLLNIKYRKKIPRDEISNFKDLLLKGRLVELTVEETEAEQDSFRRGPKRCSHCNYRGHTYQNCRKRKAEDYENCRKRKDENXSFSECNELVLTFKKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJA3</accession>
    <name>T1GJA3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sarcoglycan beta/delta/gamma/zeta family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJA3"/>
    <dbReference type="STRING" id="36166.T1GJA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003547-RA">
        <property type="protein sequence ID" value="MESCA003547-PA"/>
        <property type="gene ID" value="MESCA003547"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043450_2_0_1"/>
    <dbReference type="OMA" id="LFHESIQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006875">
        <property type="entry name" value="Sarcoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039972">
        <property type="entry name" value="Sarcoglycan_gamma/delta/zeta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939:SF10">
        <property type="entry name" value="EG:4F1.1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939">
        <property type="entry name" value="SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04790">
        <property type="entry name" value="Sarcoglycan_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003547-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="16668" checksum="C28C3C31A2C809B9" modified="2013-10-16" version="1">MGQLKIIPHGVQLTGQTLIMDLLRASSIRSKHGQPITIGKLNVPEQEFWKPYLPEKGKFVKSSRNFSINTRDKKGKLQNHLYLGHDKFECLTSGGFRITNSTGKKLFSVNQNEVFIGVPTLRIEGDGGVLFHESIQTSHVRADPGKDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJA5</accession>
    <name>T1GJA5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003550-RA">
        <property type="protein sequence ID" value="MESCA003550-PA"/>
        <property type="gene ID" value="MESCA003550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870167_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="6998" checksum="6D61A95D1483651A" modified="2013-10-16" version="1">MEGKGVGKVLAKEAIQFAKAQNLKTETFPCPCRKVNRQVSHRSLLLASLQDVGQLVVSKMTSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJM6</accession>
    <name>T1GJM6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJM6"/>
    <dbReference type="STRING" id="36166.T1GJM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003676-RA">
        <property type="protein sequence ID" value="MESCA003676-PA"/>
        <property type="gene ID" value="MESCA003676"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906659_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003676-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6806" checksum="CFDD728331EA0709" modified="2013-10-16" version="1">MAFGTMTSLLGMIPILAIGMNYFRYAKIDPENKVSEPDQQSAIDKSHRLMSLMKGAISLTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKC4</accession>
    <name>T1GKC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylinositol-3-phosphatase SAC1</fullName>
            <ecNumber evidence="1">3.1.3.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphatidylinositol-4-phosphate phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Suppressor of actin mutations 1-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:55652"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:55653"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="EC" id="3.1.3.64"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:12317"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="3.1.3.64" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02394138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKC4"/>
    <dbReference type="STRING" id="36166.T1GKC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003945-RA">
        <property type="protein sequence ID" value="MESCA003945-PA"/>
        <property type="gene ID" value="MESCA003945"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_7_4_1"/>
    <dbReference type="OMA" id="ITKAQPV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="8">
        <location>
            <begin position="127"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00036807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003945-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="591" mass="67472" checksum="4EAEA39A4B8B5BDB" modified="2013-10-16" version="1">MVISTTAQENVIYDDLFLYEDVEKLYLSPSEGNELLIIDRGNGRTEVQEKKAQIEGLRPTKRVCGVLGLINLLGGSYLVLAAHRLYVGIINGQVVWKLAGATLMPLQHQRALTAKQQNQNDTYLSMVQKILDTPYFYFSYSYDLTHTLQRLHSMPPAFHQRFVWNGFLLENFNNAGINRFALPLLLGFVSINQASLNNSSFNWIIVTRRSVQRAGTRLFCRGIDDNGHVANFVETEQIVEFNGDKVSFVQTRGSMPFYWHQTPNLRYKPPPEMIPGKDHMTSCIKHFDQQLSFYGRQVCVNLVDQKRAEGELEKTFRILITQMNNPQVRYEAFDFHKECKGMKYQRANILIDRLAQDLEQFSVFHLRSDGVLLSVQNGVFRTNCIDCLDRTNVIQSMFARRSLNDTFVKLGLLRSGNKFENVSPSFEGVFKNVWADNADLISKQYSGTGALKTDFTRTGKRTKNGLVKDGANSLARYYLTTLVMVLGRFDAIDLFLGSYKVKSGEGSLSPSPLEYAKGWRYGTFPSILLFAIAMFFASALFPTEYDTENLLFLLFWGTMVGVTTMGIFRYGVEFVDWPKLNPLKRDFQQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKM9</accession>
    <name>T1GKM9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187546">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187548">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004056-RA">
        <property type="protein sequence ID" value="MESCA004056-PA"/>
        <property type="gene ID" value="MESCA004056"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576601_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004056-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9457" checksum="314D634DDD57ADB5" modified="2013-10-16" version="1">MILPTKRPVDSDEYDSVIWKNVSLCPVALQHFSPSFYYCYVLSLEGIGLAPIKIHYIQRFTIKIDLAKKFIQKKAKFLSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL30</accession>
    <name>T1GL30_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02182285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL30"/>
    <dbReference type="STRING" id="36166.T1GL30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004216-RA">
        <property type="protein sequence ID" value="MESCA004216-PA"/>
        <property type="gene ID" value="MESCA004216"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031683_0_0_1"/>
    <dbReference type="OMA" id="AVCEARM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000001">
        <property type="entry name" value="Maltase A3, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF234">
        <property type="entry name" value="MALTASE A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="7">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004216-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="31325" checksum="7F9AE83A46546284" modified="2013-10-16" version="1">MIKKANEIGIKIILDFVPNHTSDEHEWFIKSENREKGYEDLFMWDDGKVDPSNPNNRLPPSNWISVFRGSAWTWSSKRKQFYYHAFLDKQPDLNYRSPTTVQLMKNALKFWLDRGVAGFRVDSVPNLFEVAPRNGVYPDEPINLSQPDEDNYDHLLHVYVTDQPETIDMVYQWREVLTQHEQAHGGDERILMIETYSVPAYSNQMYGNKTTEGAQIPFNFNLITKVHQDTNAQGVVDAIDAWMQAMPSGKTANKGRLEIMIKKEHQQGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLE8</accession>
    <name>T1GLE8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposable element P transposase-like RNase H C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02008605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLE8"/>
    <dbReference type="STRING" id="36166.T1GLE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004349-RA">
        <property type="protein sequence ID" value="MESCA004349-PA"/>
        <property type="gene ID" value="MESCA004349"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559151_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048367">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21789">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposable element P transposase-like RNase H C-terminal" evidence="1">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004349-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="20003" checksum="05E173F475A9639A" modified="2013-10-16" version="1">NNFAKNIGNFFDTENTGSVHLTLWSPIGVTNDWFDVFNSSIPKKDSRQRTWAFGLSLDIQMEILDKTIGLYQSINRRHKTRDGQYRIGSIAPFQKGIIMNCRTLPLLLEDMKKKYGAKYILTRRLNQDYLEAFFGQIRAMGFQHGHPSPLEFCSRMRKYLLADISKDTIFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLF2</accession>
    <name>T1GLF2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02035503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLF2"/>
    <dbReference type="STRING" id="36166.T1GLF2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004353-RA">
        <property type="protein sequence ID" value="MESCA004353-PA"/>
        <property type="gene ID" value="MESCA004353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022631_0_0_1"/>
    <dbReference type="OMA" id="CLQYFPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058698">
        <property type="entry name" value="CUB_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33236:SF12">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33236">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 122 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26080">
        <property type="entry name" value="CUB_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577493" description="CUB domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="29"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="158"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="298" mass="32860" checksum="13B5E231EF2D387E" modified="2013-10-16" version="1">MSKTTLILVALACVGYANAGIFGGSKIECGSSTDKKSFTLINPSIPPNSCNYKVEAYSSYVCQLRIEFDMQLAQPTLPSAQNGLEYPECVDDYLEVGGYKFCGRELTAHRAGGSQLPTIVWDIRVEQLECPKGSPVRAFPIAQRASFTDGWWIAPVGCLQYFPDSTGTVTSFNYDNGLGTYQGNFDYAICFKRETSTEGIELDFDSFELGYRSDINEELGEACRPAQRNSGFNEDRLSIPDAEVEEDNINGNLFCGSSLSGKTVTSRAPGPLVLRFHSDKIYRADIPERGFTFSYNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-11-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLI9</accession>
    <name>T1GLI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004393-RA">
        <property type="protein sequence ID" value="MESCA004393-PA"/>
        <property type="gene ID" value="MESCA004393"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_797629_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004393-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="348" mass="38900" checksum="FFEE71062C811FB0" modified="2013-10-16" version="1">MFDNKRWKTKLKVVPTGFRSSAGMLSLPMPLLFLSLWICLSTSVLDGGAHHLGVVRGDVDTPTFLFQLRISILIQVDVLNSKGFPTLTIPFNFLIKFFPCLSKVSIYCSRILKWKARVMIFLLLNHFLPLLTSNPSFSHGSKSLYFSALSLNLLPPRINSTSLRSLVKMLNSGPIQTYNILIVLTFLGFQLIRNKSRCKSPPNTNYQQCLKKNVIIPIVKFQTRQVQWHPSPQSLPTITKSPDIICLSVHLLNTLIGDSTLLPNIFKLSSTGVTEAFLASSSSISAHSISKCPGNQQSLSELTLLSSFSDSSHVSTTPEDILKMHAMPPDYQYGSLCYSYLRRLYIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLV3</accession>
    <name>T1GLV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02108066">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02108067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLV3"/>
    <dbReference type="STRING" id="36166.T1GLV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004517-RA">
        <property type="protein sequence ID" value="MESCA004517-PA"/>
        <property type="gene ID" value="MESCA004517"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005966_2_0_1"/>
    <dbReference type="OMA" id="RPTIRIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00175">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000001">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="269"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004517-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="618" mass="70076" checksum="099CD7EAED724E51" modified="2013-10-16" version="1">MSRPSPQDFDIISPQLHEETKDDPFAWEAREALRKRLIGEEVVFTFEKPANSNREYGVVYLGKDINTGENIVEYMVSEGYLNVRKESRSSPELTRLVELEEAAKSAGKGKWNPNTQSSDHVRQIKWTQENPAQVVEHYGGKPVKAIVEHVRDGSTIRAFLVPDFYHITLMISGIRCPGVKLDADGKPDLTVKVGICQQRQFVGTILYPKGNIAEALLREGFAKCVDWSMAFMKSGADKLRAAERQAKEKRLRLWKDYQSNAQQFSGKEKDFTGTVVEVFNGDAINVKTPNGAIKKVFLSSIRPPREAGRVADEDGKLPPRPKNFRPLYDIPWMFEAREFLRKKLINKKVNCNLDYITEARDNFPEKYCYTVTIGGQNIAEAMVQKGLATLVKYRQDDDKRSSKYDDLLTAEAQAIKGQKGLHSKKDRPTIRINDLTVDHSRIKHQYLPSWTRALRSEAVVEFVASGSRVRLYMPKESCLAVPFSRVAALPPSVSSPKPFAVEYVLAFCQLPPDSDDKDEALRALADDVLNRKVLMNIESHRRNTNNLPEATLHNAETKADIGKSLIQEGLLIVEKRSERRLKEIIGEYKEAEASARKEHYGIWQYGDVTQDDAPEFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM73</accession>
    <name>T1GM73_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199545">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM73"/>
    <dbReference type="STRING" id="36166.T1GM73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004641-RA">
        <property type="protein sequence ID" value="MESCA004641-PA"/>
        <property type="gene ID" value="MESCA004641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707639_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062:SF1">
        <property type="entry name" value="LP12374P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8453" checksum="0690F6F67AF05F6F" modified="2013-10-16" version="1">MCFFKFFTAMGNIFSWQNISTSLIIWTLNVMVLLGCLVKLLVYGDPVLKSESKANQEYWKHKKKADAEFSKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GML0</accession>
    <name>T1GML0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02052988">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GML0"/>
    <dbReference type="STRING" id="36166.T1GML0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004786-RA">
        <property type="protein sequence ID" value="MESCA004786-PA"/>
        <property type="gene ID" value="MESCA004786"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203566_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537:SF8">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004786-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12749" checksum="365061C01C6D7F52" modified="2013-10-16" version="1">VFHAPDLQEEKPFEFRIRYKFISQSDAVVRYGLPDTLLELGRVTPGTYCTRQFDECHRNKCRLQSPNYPGMYPRNVTCYWTIRQKEVPTMKHAMIAVSQENQHKALVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GML6</accession>
    <name>T1GML6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02044700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GML6"/>
    <dbReference type="STRING" id="36166.T1GML6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004792-RA">
        <property type="protein sequence ID" value="MESCA004792-PA"/>
        <property type="gene ID" value="MESCA004792"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1437841_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004792-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="21352" checksum="BA537375AEEEB45F" modified="2013-10-16" version="1">RAQFNFDKEKDTQKVVIQKIINVFAPKFEKFHEFLLNPPVARTIQTTTGVLAPPFGLTRLQICRLARVLLTTGNETMVQAIANTDFMVTLLNFFKEYCWNNFLHEEVEKCLEHIFASKSCNLDTTIWNSTATDINGTMESDQNAENEIKVQNFEESGGGGGNSQLNSDQTSDSKTTNTDTKEITPLQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNR3</accession>
    <name>T1GNR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNR3"/>
    <dbReference type="STRING" id="36166.T1GNR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005222-RA">
        <property type="protein sequence ID" value="MESCA005222-PA"/>
        <property type="gene ID" value="MESCA005222"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_802376_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000273">
        <property type="entry name" value="Receptor protein-tyrosine phosphatase 10D, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="187"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005222-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39909" checksum="DE5BA7B9141CB435" modified="2013-10-16" version="1">MSEDEISIPTTAQYRTVPLRPTNVTIDRDTITSNSFKVVWESPKGVSEFDKYQVSLSTSRRQSTVGRTNEPKASLEFRDNLEPGKTYSIVVKTVSGKVTSWPATDQDNTSLILAFTIPLAIILVLVATFILFKKRRISSGCRKTVKDSNGNDNMSLPDSDIIQNRPVLIKNFAEHYMLMSADSDFRFSEVFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDEEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDHYWPHDTMPVFYGDIKVQILNESLYSDWNTTEFLLCRLDNDRIPTRETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNR9</accession>
    <name>T1GNR9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02069472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005228-RA">
        <property type="protein sequence ID" value="MESCA005228-PA"/>
        <property type="gene ID" value="MESCA005228"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852254_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7474" checksum="6D7B65FC2D64E796" modified="2013-10-16" version="1">MVPKRLVACKCTYSAFSFPSIPRSLLPLPRQCILGSKDTMSIRKKSKFVFAPFMPRPLVREKLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNS6</accession>
    <name>T1GNS6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MIF4G-like type 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNS6"/>
    <dbReference type="STRING" id="36166.T1GNS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005235-RA">
        <property type="protein sequence ID" value="MESCA005235-PA"/>
        <property type="gene ID" value="MESCA005235"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1143692_0_0_1"/>
    <dbReference type="OMA" id="NMFELWH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 2" evidence="2">
        <location>
            <begin position="18"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27607" checksum="2D0F2A83AB33CBAE" modified="2013-10-16" version="1">MMPETYSKLLPAIAQPTYKYSSENASSLPGTQVALQLILAIRQKCTPEEELPNPNESMETDNAEESFNPLKIDVFVQTLLNLGSKSFSHTFAAISKFHTVFRALADTEEAQICVLHNVFELWKHHQQMMVVIIDKLLKLQIVDCSVVATWIFSKEMTSEFTKMYLWEILHLTIKKMNNIDDEDEQSNKNKNDNVDKPTEEGPKQASSKPPNILSEANDGTNNDEDTKDKSKCYELFPRETTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP16</accession>
    <name>T1GP16_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit gamma, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02095507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP16"/>
    <dbReference type="STRING" id="36166.T1GP16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005331-RA">
        <property type="protein sequence ID" value="MESCA005331-PA"/>
        <property type="gene ID" value="MESCA005331"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2190541_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005331-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12026" checksum="541BA462385BCDBD" modified="2013-10-16" version="1">MKMVSAAKYARAERDLKQARPYGEGAQQFFEKAEVAPAETVEKKKLLIAMTSDRGLCGAVHTGVARTIRNELNEGNNADNTKIICIGDKSRSILQRIFGKNIIFVANEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPG8</accession>
    <name>T1GPG8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPG8"/>
    <dbReference type="STRING" id="36166.T1GPG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005498-RA">
        <property type="protein sequence ID" value="MESCA005498-PA"/>
        <property type="gene ID" value="MESCA005498"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880599_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15550" checksum="5499FE82D8A7CCC2" modified="2013-10-16" version="1">IIPISVEGGGLIKPSTSTASSEPKKNPFEDKFEHTFYNTGFRNNNLMRSRFSTKRRDDSESQSSDEEDDGFEILSAEKLFSTLLQRVKDLTNRLNINDVHYRGIANNSIFNNASVMPPTNWKFNRKLKVSQPFSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPQ3</accession>
    <name>T1GPQ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024531">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024533">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024534">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPQ3"/>
    <dbReference type="STRING" id="36166.T1GPQ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005592-RA">
        <property type="protein sequence ID" value="MESCA005592-PA"/>
        <property type="gene ID" value="MESCA005592"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_998497_0_0_1"/>
    <dbReference type="OMA" id="NETFYPN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF205">
        <property type="entry name" value="GH28721P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005592-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="31987" checksum="671D9667AC8494EC" modified="2013-10-16" version="1">MASSQSVRNQRIAMSFGFVILVIVLLYCLIGTVYSGAISRSRPARFQQSSKKDTLQLLHVVFRHGARTPADTYPLDPYFNETFYPNGWGQVFRHGARTPADTYPLDPYFNETFYPNGWGQVTNDGKDKLYKMGIWLHDRYGKFLGEYMPDSVYAQATGVSRTHVSLQTVLAGLYRPRNTALDWNRNLNWLPIPVFSQPLEEDSLLLVRTPCPRYYEARDDVYKLPEIASLHEKYKDMMKELSDHTGKHIENAEDVADVYTTLLAEKEFACPFLGPHVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPY6</accession>
    <name>T1GPY6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPY6"/>
    <dbReference type="STRING" id="36166.T1GPY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005682-RA">
        <property type="protein sequence ID" value="MESCA005682-PA"/>
        <property type="gene ID" value="MESCA005682"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1024105_0_0_1"/>
    <dbReference type="OMA" id="CERSGAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="11">
        <location>
            <begin position="86"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005682-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="272" mass="31136" checksum="2E82DFF5D33721AB" modified="2013-10-16" version="1">MIEVVFQVRKDGFKDHQSVIESLELVEEADQFTHFLMVDDVTTTEDVISNEEVRRTDDSNLITEFHNEQKIQNDSSLDVISSPPPREALITTAINFLQNERIRKSPINQKQKFLMSKGLTEQEIQIAFNRAGVFTFDPNHAEMGIAAQSNYLAISQENKSLFGKIKEAIQSMALISAVAYACYLFYKKYIERFLFGKKKKTVEEHLLSLEQQMDKKINDLSKEFTIVKDVVISNSHTEDVSQKLQSFKNDLDAIKGLLLNSIEFINCITLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQU7</accession>
    <name>T1GQU7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQU7"/>
    <dbReference type="STRING" id="36166.T1GQU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006012-RA">
        <property type="protein sequence ID" value="MESCA006012-PA"/>
        <property type="gene ID" value="MESCA006012"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1143687_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="25335" checksum="741E0AEFE14FB033" modified="2013-10-16" version="1">MVADFNDPDVGPGVPYVFKDEDENQASDPKGIVESSSNKDEVVVSTTTPTPILSSTTATPAQTFSVPTKGNKGHLASNSVFPSTATAITTHFQKASQHQQQNFQSQFISSSQNQNRVQVNRNSLSSTASSTFSSSPSPFKQSSFGSTSFQASTSPSFNQIPNSSTKFTGRFGGAPGILGEQVTPGFGVRPSAFSKPTTTPATLAPAASNVVERRVTSGSGRYTGGFGGPPGILVPFDNVKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR75</accession>
    <name>T1GR75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02169730">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR75"/>
    <dbReference type="STRING" id="36166.T1GR75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006151-RA">
        <property type="protein sequence ID" value="MESCA006151-PA"/>
        <property type="gene ID" value="MESCA006151"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_12_1"/>
    <dbReference type="OMA" id="HENAFRR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17993">
        <property type="entry name" value="DEXHc_CHD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000007">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="53"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006151-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="298" mass="35244" checksum="29DB88BF2BA4F563" modified="2013-10-16" version="1">MFNEREKSQNIPTRHAKVLKYRPKFVPMDSQPSFLSSGLVLRDYQVDGINWLIHSWCKNNSVILADEMGLGKTIQTICFLYYLFKCQHLYGPFLCIVPLSTMTAWQREFTIWAPDINIVTYLGDVQSREIIRQYELYSDEQTGNRLKFNAILTTYEILLKDKMFLGSIQWSVLLVDEAHRLKNDDSLLYKTVKEFDTNHRLLITGTPLQNSLKELWALLHFIMPSKFETWEDFEEDNENAADKGYTNLHKQLEPFILRRVKKDVEKSLPAKVEQILRVEMTQTQKQYYKWILTKNLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRH3</accession>
    <name>T1GRH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRH3"/>
    <dbReference type="STRING" id="36166.T1GRH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006258-RA">
        <property type="protein sequence ID" value="MESCA006258-PA"/>
        <property type="gene ID" value="MESCA006258"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105559_0_0_1"/>
    <dbReference type="OMA" id="DFAMCEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="142"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006258-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="18936" checksum="30710710C68D105C" modified="2013-10-16" version="1">MYTFTPCDRKKCFPVENRYIDGHLQDFTALGSYFSSWSMETEFLEAISDFHLLIYLYKMEMLPMKTQMQSLLEAVRTKNKDQAYEFKKNETWTLLESLVGNEVGMSEGAGPSRKNGATGRGSSISSENLSNSGDNDGAAGSGAGGSVSWTCDHCTFINGSVLSSCEMCGLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS94</accession>
    <name>T1GS94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphorylated adapter RNA export protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA U small nuclear RNA export adapter protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PHAX family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS94"/>
    <dbReference type="STRING" id="36166.T1GS94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006545-RA">
        <property type="protein sequence ID" value="MESCA006545-PA"/>
        <property type="gene ID" value="MESCA006545"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048946_0_0_1"/>
    <dbReference type="OMA" id="HAINEVD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006408">
        <property type="term" value="P:snRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1440:FF:000001">
        <property type="entry name" value="phosphorylated adapter RNA export protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1440">
        <property type="entry name" value="PHAX RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039047">
        <property type="entry name" value="PHAX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019385">
        <property type="entry name" value="PHAX_RNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038092">
        <property type="entry name" value="PHAX_RNA-binding_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13135">
        <property type="entry name" value="CYTOSOLIC RESINIFERATOXIN BINDING PROTEIN RBP-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13135:SF0">
        <property type="entry name" value="PHOSPHORYLATED ADAPTER RNA EXPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10258">
        <property type="entry name" value="PHAX_RNA-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphorylated adapter RNA export protein RNA-binding" evidence="12">
        <location>
            <begin position="101"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="30"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="208"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006545-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="343" mass="39336" checksum="606EA712EAA19AA9" modified="2013-10-16" version="1">MRGIGVAHGSRDDRSVETYDYKLKYRLEGRKRALSDNDQSDDSEGPSNKKSKCNNSSRMKKRPVKERLGSKKHSSESSDFENEPRILCDLTLTENDEAESLAREIAHNLFEEKDELILRLVSVVGTRMAIKLFQETQKIEKEGGMMIVNGKRRRTPGGVFFFLLKHDNNVTSDQRKMIFYEENKIAAKDRKSLQAMKRDQKVEELKKRLTEQDHLPLPNISSASSNENSVNLSNPPPSPVVGHDNSPEYKSHAINEVDKLEESVFNEAEKLEATEQSRNDSGLDGLTSYDDDFLDITCDDMELCKGIFLNKVSYKTDFLIRSLVFKEITKHEITHNKKSYIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSW0</accession>
    <name>T1GSW0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006772-RA">
        <property type="protein sequence ID" value="MESCA006772-PA"/>
        <property type="gene ID" value="MESCA006772"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113867_0_0_1"/>
    <dbReference type="OMA" id="FELMMIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF12">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006772-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="24830" checksum="0A9FF317E0FE2F36" modified="2013-10-16" version="1">MFRDGFKNFLELLKSNPEFSEYVPKFEKIVENKPLQKATKLIKAVSNGDKVHLLVLNHGDFHIKNIMYTEKDGELDDVRLIDFQGSAWGPAVFDLTYMLYMLLDDESRLNRRNEIIHYYFTHLIESLKNLKFEGELPKLTELYKDFLTYKDFELMMITTMLPFMTAFSNDDFDLNTMSNENMITGLYHNPKYVNFVRKALPDLLYKGYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSW7</accession>
    <name>T1GSW7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM41 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02184048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSW7"/>
    <dbReference type="STRING" id="36166.T1GSW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006779-RA">
        <property type="protein sequence ID" value="MESCA006779-PA"/>
        <property type="gene ID" value="MESCA006779"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038944_0_2_1"/>
    <dbReference type="OMA" id="CIKIPRD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045014">
        <property type="entry name" value="TM41A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220:SF18">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 41B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="25"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006779-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="20274" checksum="51EC49147BC3F617" modified="2013-10-16" version="1">MPQNQLPAGMVEYDLSSCSLQTFAIPGSLFLSILLGFLYKFPVALSLICFSSAFGASLCYLLASVVGRPLILHYCSSRTTTLTNYVNKHKDSLFNLMLFLRITPILPNWLINLCAPVVGVPLYPFVLGTFFGVAPPSVLAIQAGKTLQTMTTASGTFSWTNVAILSASAALAIVPIFFKNKVKNKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTE0</accession>
    <name>T1GTE0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTE0"/>
    <dbReference type="STRING" id="36166.T1GTE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006970-RA">
        <property type="protein sequence ID" value="MESCA006970-PA"/>
        <property type="gene ID" value="MESCA006970"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037158_2_0_1"/>
    <dbReference type="OMA" id="ENQHESK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000068">
        <property type="entry name" value="Islet cell autoantigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024114">
        <property type="entry name" value="Islet_autoAg_Ica1/Ica1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006723">
        <property type="entry name" value="Islet_autoAg_Ica1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164:SF4">
        <property type="entry name" value="GH23156P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164">
        <property type="entry name" value="ISLET CELL AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04629">
        <property type="entry name" value="ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01237">
        <property type="entry name" value="ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006970-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="359" mass="40919" checksum="EE913A593F1DCE60" modified="2013-10-16" version="1">MGKFLKEAGKRSNTTGKNMINTGKSISYCGQQRMTIRIPLLRLHHEVEVFQCRAVADTQHTLNNMEKERTEYRAALSWMKSASTQLDPDTGKGLEKFRKAQTHVRDAKKKFDRLSMDCIQKIDLLAAARCNMFSHGLVGYMNALLQFSKKASETYDLITSVISMKTQYEFTVLKDLKQEHIPNNDETSQDKVISKMDNDQMFFFQDDYEDKEVPIEENNCSVLKENQHESKVQNILIDNYKCDKCLSDKNDITEEILKDDPFPSLLLDDIPNKSSTDLDLLHLIPSSSALIGNPVQTNILQTNTYLINDNKQSKTSVNVKKEDTQKLSSWLELFADLDPLANPELAKKISGNEAYSQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTX7</accession>
    <name>T1GTX7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GTX7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007175-RA">
        <property type="protein sequence ID" value="MESCA007175-PA"/>
        <property type="gene ID" value="MESCA007175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025000_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12352">
        <property type="entry name" value="RRM1_TIA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12353">
        <property type="entry name" value="RRM2_TIA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000419">
        <property type="entry name" value="CLUMA_CG006354, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000087">
        <property type="entry name" value="Nucleolysin TIAR isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000317">
        <property type="entry name" value="Rox8, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="95"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="243"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="434" mass="47397" checksum="ECC2FA5EE540CE3B" modified="2013-10-16" version="1">MDDTQPKTLYVGNLDQSVSEELLVALFATMGNVKTCKIIREPGNDPYAFIEYASHQSAATALNAMNKRVFLEKEIKVNWATSPGNQPKTDTSSHHHIFVGDLSPEIETDFLREAFAPFGEISNCRIVRDPTTQKSKGYAFVSFVKKAEAELAIQTMNGQWLGSRSIRTNWSSRKPPPPRDNKSDSKGSNNNGNXXXXXXXXXXXXXXMFAGSNNNGNGSIGSNKLSNKSTYEEVYSQTTPTNTTVYCGGFPPYVITDEIMHKHFSTYGHIQDVRVFKDKGYAFIKFTTKESAALAIEQTHNTEISGYAVKCFWGKENGGFPSNSSNLAGGDNSLNSTNSSMVAGLGLGAGHMAGLGHGNVQANLLANHQMNAQVLTPQQAAQYPYAAAAAAYQQMGYWYAPAGYSTQMQGPYMQQGYYPYAAYTSAQQSGKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU29</accession>
    <name>T1GU29_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392031">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392032">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007230-RA">
        <property type="protein sequence ID" value="MESCA007230-PA"/>
        <property type="gene ID" value="MESCA007230"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815350_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007230-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7371" checksum="4829184BD9859C81" modified="2013-10-16" version="1">MVCIIPAGQHVFGFFSINIQSPRLGFIFLKGNLDVCCCPTNQINIVAITNYDEGTATTSLRVHLKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUB0</accession>
    <name>T1GUB0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007320-RA">
        <property type="protein sequence ID" value="MESCA007320-PA"/>
        <property type="gene ID" value="MESCA007320"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742953_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007320-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7729" checksum="C79B86CCE586B6D9" modified="2013-10-16" version="1">MDLRQTVENAIATFYKSVDELKTNPVVMDINQYGSQAFFAVYKGALDLIRLLIPLASPDAHVEGEGDAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUJ2</accession>
    <name>T1GUJ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUJ2"/>
    <dbReference type="STRING" id="36166.T1GUJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007406-RA">
        <property type="protein sequence ID" value="MESCA007406-PA"/>
        <property type="gene ID" value="MESCA007406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_1_1"/>
    <dbReference type="OMA" id="FKMHRQP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF106">
        <property type="entry name" value="CYTOCHROME P450 4G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="56473" checksum="A139AF235B1E51D1" modified="2013-10-16" version="1">MYQLAERLPGIKGLPIVGNLFDVFGTSHSVFKTVTTKGKHFYEVCKLWIGPRLVVFLFDPRDVEILLSSQTYIDKSSEYDFFKPWLGEGLLISTGYKWRSHRKLIAPTFHLNVLKSFVELFNENSVNVVKKLRKEGGKTFDAHDYMSEATVEILLETAMGVSKKTQNKSGYEYALAVMKMCDILHIRQRSLFLRNELIFILSKYYRKQGKLLDIIHGLTTRVIKSKKEAFSKGNRGSLAEISQHFAEDPGLTKNKDTVEGLSYGQSAGLHDDLDVEENDVGEKKRLAFLDLLLESAQNGALISDTEIKEQVDTIMFEGHDTTAAASSFFLSLMGIHQHIQDKVIAELDQIFGDSDRPATFQDTLEMKQLNEDLQLASNKDYIVPAGATIVVGTYKLHRQPTIYPNPDVFDPDNFLPERQANRHYYAFVPFSAGPRSCVGRKYAMLKLKILLSTILRNFRIHSDLTENDFRLQADIIMKREEGFKVRLSPRRPAKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUK9</accession>
    <name>T1GUK9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUK9"/>
    <dbReference type="STRING" id="36166.T1GUK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007424-RA">
        <property type="protein sequence ID" value="MESCA007424-PA"/>
        <property type="gene ID" value="MESCA007424"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1492689_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="19760" checksum="F70EF2CB49C82AB2" modified="2013-10-16" version="1">FRYLVRPSPFSAQKTFERHNSRRKSNISFSNLNVASGVSGAGSRRGSTLALNQLQLQAIPSLSGYGPPRAPPPIQQIKSNSLSLPDSPNNVVGTTRARSNSLRIDSDVLLRRSSSLRQGLPNVGINTGRRKSSLEEIGISHFTTLMQATNHSNPIKIALNGSIGLEDSQLKEVYKLCIWHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVC7</accession>
    <name>T1GVC7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007719-RA">
        <property type="protein sequence ID" value="MESCA007719-PA"/>
        <property type="gene ID" value="MESCA007719"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608762_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007719-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9252" checksum="9D0B7758A22598C4" modified="2013-10-16" version="1">MNSGQKFPFKILERKTDRQLHEKWAEISFRPKNENHSLLLSRITTEFSEIPSGISFIFEHFKGMGTACNVVVLYFIVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVL3</accession>
    <name>T1GVL3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007824-RA">
        <property type="protein sequence ID" value="MESCA007824-PA"/>
        <property type="gene ID" value="MESCA007824"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852281_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007824-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7518" checksum="A6401BA738087D78" modified="2013-10-16" version="1">MRWMPLHGAKRSVWHESVLNTQNLSGCFFSHSIMWSCDKIIDVDDADGCQLAFLELMLTLIYKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVR4</accession>
    <name>T1GVR4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVR4"/>
    <dbReference type="STRING" id="36166.T1GVR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007880-RA">
        <property type="protein sequence ID" value="MESCA007880-PA"/>
        <property type="gene ID" value="MESCA007880"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066247_9_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="6">
        <location>
            <begin position="45"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007880-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13155" checksum="6EA59A36A5B87B27" modified="2013-10-16" version="1">MDNLDNLDDYIIMEDENDDEDNEISDESGSGKHGSTSMLREQDRFLPICNIIKIMKMTIPDNGKIAKDARECIQECVSEFISFITSEAIDKSYSENRKTVNGDDLLYAFIALGFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVW1</accession>
    <name>T1GVW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02199606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVW1"/>
    <dbReference type="STRING" id="36166.T1GVW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007929-RA">
        <property type="protein sequence ID" value="MESCA007929-PA"/>
        <property type="gene ID" value="MESCA007929"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_2_1_1"/>
    <dbReference type="OMA" id="RTSEMVC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="8">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007929-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22046" checksum="C4F77C47754DD05B" modified="2013-10-16" version="1">QIGDKFSSRHGQKGVTGLIVEQEDLPFNDFGICPDMVMNPHGFPSRMTVGKTLELLGGKAGLLEGKFHYGTAFGGSKCSDIQEELFKHGFNYMGKDYFYSGITGESLEAYIYSGPVYYQKLKHMVQDKMHARARGPKAVLTRQPTQGRSREGGLRLGEMERDCLISYGASMLIMERLMISSDAFEVDVCTPPPIFQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWE8</accession>
    <name>T1GWE8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391646">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008122-RA">
        <property type="protein sequence ID" value="MESCA008122-PA"/>
        <property type="gene ID" value="MESCA008122"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471625_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008122-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10475" checksum="13E5407791429D24" modified="2013-10-16" version="1">MGPKALYDSRCTPRYASSSKQKFEKRHNKILVGFFMSLVQSKNRDRNGDLITNKNEVLMQWEEFFSELLNDNKQNIEEQAPFVFQKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWL8</accession>
    <name>T1GWL8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxiredoxin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWL8"/>
    <dbReference type="STRING" id="36166.T1GWL8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008195-RA">
        <property type="protein sequence ID" value="MESCA008195-PA"/>
        <property type="gene ID" value="MESCA008195"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204149_0_0_1"/>
    <dbReference type="OMA" id="VNWTPGT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxiredoxin C-terminal" evidence="4">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008195-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7657" checksum="EB91629BB2DBA7E2" modified="2013-10-16" version="1">MTFFELTSDATSIDKTYKVVATPVNWTPGTKVIILPHVSYAEAEKLFPGAVDMVSMPSGVNYVRTIEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX02</accession>
    <name>T1GX02_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX02"/>
    <dbReference type="STRING" id="36166.T1GX02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008339-RA">
        <property type="protein sequence ID" value="MESCA008339-PA"/>
        <property type="gene ID" value="MESCA008339"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944321_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577721" description="Peptidase S1 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008339-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="6817" checksum="6C32242F76C62666" modified="2013-10-16" version="1">MLLKFVIFFVVSTTFVECYSIGHVKNSNTVEYPYQVMLIGRSMWSNRILCGGTLLTTSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX21</accession>
    <name>T1GX21_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008359-RA">
        <property type="protein sequence ID" value="MESCA008359-PA"/>
        <property type="gene ID" value="MESCA008359"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500483_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008359-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9938" checksum="AFC2DFA91913F0DA" modified="2013-10-16" version="1">MNSTMVSYWLPLSLTCLLEPFQKITGHQESARSIDFLVLERISVNLFHSKLTYKNTTRKFSSDMVESGSVQFWKLPTPFNTKSFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXC6</accession>
    <name>T1GXC6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD repeat-containing protein 55 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXC6"/>
    <dbReference type="STRING" id="36166.T1GXC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008472-RA">
        <property type="protein sequence ID" value="MESCA008472-PA"/>
        <property type="gene ID" value="MESCA008472"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1205871_0_0_1"/>
    <dbReference type="OMA" id="ATERDYS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052301">
        <property type="entry name" value="SCF_F-box/WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381">
        <property type="entry name" value="DACTYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008472-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="26130" checksum="D88724DB48B98659" modified="2013-10-16" version="1">MFTGHHHVAIVSVQTGQYYASGSDQRFKIWKKEVEFDELVSLDLQYNFWNYFMCAKISRGKVFGGLYADVQQRRALREIDIETGNVDILHCNSSLKIKDDNILYTGHFDTTTRVFDRRTDTDEIIFIDPFDSSAYCIDHDGKYALLCGTKHHSRVNLYDIRVPNRVAQIYYPVRQKRKESSPVYSLCCDSRFLFIATDINLRVFDFKADWAKVKDYSNIISKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXE7</accession>
    <name>T1GXE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02168206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXE7"/>
    <dbReference type="STRING" id="36166.T1GXE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008495-RA">
        <property type="protein sequence ID" value="MESCA008495-PA"/>
        <property type="gene ID" value="MESCA008495"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629428_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035871">
        <property type="term" value="P:protein K11-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF27">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008495-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="436" mass="49171" checksum="1F2FC80FE0B55622" modified="2013-10-16" version="1">MVKNYLDCARNRFEASKSYNVDFDNNLICITPGCNNIYRSEENRLCFDCKELSDKQEDNGPALRYGTGNSKFYTHVDNSHHEKVKNLQPTRKPSDFDQTLYLSNSTFYNDQVPEFLNQHHEFSTESSPCRNAGSGFVYSESEWAKEWFELVSMASTEPRTKSRSRRISTTSNSNLTPSNSVDDCAIYESLEEIHILALAHVLHRTIISTDCSRGPLILAYDMAHFSALVPMDTGDDLPALIPLVSSSNTFLPIQFCIDPGENFTWSTYDGTEDCWALTEREQLALLKEYLDIVCKASSPHDIEIDFSEDYTDEELTRFQEAELIISTTVNLPDENGTITTFGRSNGNSKTTKQLQNVARSFGSIGKNMDIGSITRIGSSKGSSGSKNKHSTSTQSFTSSKIVCARLRIEKSTFQEEMVKNYLDCARNRFEASKSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY30</accession>
    <name>T1GY30_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02106275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02106277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008750-RA">
        <property type="protein sequence ID" value="MESCA008750-PA"/>
        <property type="gene ID" value="MESCA008750"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725087_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008750-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8232" checksum="55F4DE4C65767AF8" modified="2013-10-16" version="1">MLTDRQNHKEFCDAICSIVPRNDLDSNLEYVTMTQIATCPLSALYFLKLSGQQVWKTLTIHLVGAELEFEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY52</accession>
    <name>T1GY52_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BOD1/SHG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY52"/>
    <dbReference type="STRING" id="36166.T1GY52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008772-RA">
        <property type="protein sequence ID" value="MESCA008772-PA"/>
        <property type="gene ID" value="MESCA008772"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_975111_0_0_1"/>
    <dbReference type="OMA" id="SENAQMP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055264">
        <property type="entry name" value="BOD1/SHG1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31532">
        <property type="entry name" value="BIORIENTATION OF CHROMOSOMES IN CELL DIVISION 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31532:SF10">
        <property type="entry name" value="BIORIENTATION OF CHROMOSOMES IN CELL DIVISION PROTEIN 1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05205">
        <property type="entry name" value="COMPASS-Shg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BOD1/SHG1" evidence="2">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008772-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="286" mass="32450" checksum="5941AA980B58F80E" modified="2013-10-16" version="1">MESTADKIVAEVKSQGVFDQFRRDSIAQVDTKPAYINLIQRVQSTVNKFLSSKVWGPEMNKIQMLVNPKIATTFQPQIEKIAYGTLGIEKPKIKEEDKKSFQDFSSTMSLVIPNGNLMPEELEQISPDSDKMTIKSESKGSDSMDTSDDKTEDFESPEFEPIENNSNDNLEAEKKPEIKLEDKLEIKTENEKNEVTQESNLSQVSSLLSIISESEDTNLHASISENAQMPKFNENSNSFTEASTKTSNFTELAFNIKKDEIKFEGTERKMNFSEEKLEEIVKTEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYP5</accession>
    <name>T1GYP5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYP5"/>
    <dbReference type="STRING" id="36166.T1GYP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008981-RA">
        <property type="protein sequence ID" value="MESCA008981-PA"/>
        <property type="gene ID" value="MESCA008981"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1820629_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008981-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16425" checksum="B5BCDF676B099C8A" modified="2013-10-16" version="1">MFSTVLRYQFRRTILSSNISSIDNRYRTLFTTNALFKRRKTAEEKKSPKIIEYSPKKSANKTKVFGTVDIWRNITVRELANACDKSLDDVQEVMLYVKGADDIEPSARLEDIKIIREIVQKCGLKSRIVAAPEKEEDHDIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYP6</accession>
    <name>T1GYP6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008983-RA">
        <property type="protein sequence ID" value="MESCA008983-PA"/>
        <property type="gene ID" value="MESCA008983"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_920013_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008983-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="303" mass="35304" checksum="E8850477B7EE6138" modified="2013-10-16" version="1">STDNLIFWSQTQKDQLARHELRARTDQKKIKDEIENLKSGLNKIEETKLMNENNEIHLISNKLSRLSEDVYIFIEKQNSSNVNLFNTVQKSISDTLNEIAGGLTDVDKGVSKIIQETTLQGRIFIEKLSNETNAKIEDLKNELLVSFKDVILENIKKVNIKIDSENNKIEEIQNGFLLEIENKFKKLNTTIQKSSSKYEQEFQELNIYLQNTQNIIGKSEAKLEDMKNELTDNFHNEFRALNFSVNNINEKITELDVELDYKENRTLKEIQNIKLDSKDILGIVLKILVLLVKEFMIQWLRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYR2</accession>
    <name>T1GYR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="2">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02375316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYR2"/>
    <dbReference type="STRING" id="36166.T1GYR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008999-RA">
        <property type="protein sequence ID" value="MESCA008999-PA"/>
        <property type="gene ID" value="MESCA008999"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001613_7_1_1"/>
    <dbReference type="OMA" id="ACKHERW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18037">
        <property type="entry name" value="DEXSc_Pif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003367">
        <property type="entry name" value="ATP-dependent DNA helicase PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057437">
        <property type="entry name" value="PIF1/LRR1_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF7">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25344">
        <property type="entry name" value="PH_LRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIF1/LRR1 pleckstrin homology" evidence="5">
        <location>
            <begin position="14"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="3">
        <location>
            <begin position="189"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="4">
        <location>
            <begin position="427"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008999-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="643" mass="71439" checksum="9B38105B25F70F9C" modified="2013-10-16" version="1">MDQQCWQPGRKLFYKTATLRLIRSDLKEIFIEVTPEKVKPIQFRLKEFNIHKKFMAEGKASITFKTERLTMFLSNAPPGTLIMFLKTIFIKMTGGEKPDDASTLKNIRAMMLNGKPASFNDISPVTTAEMVMARKAAGVSKSTLTTPSPPQAKKRRFEELKASGEITGPAPKKLYQSSPLVEDSIKLCPEQMEVLNSCVAGKNVFFTGSAGTGKSYLLRKIISSLPPDGTMATASTGVAACLIGGVTLHSFAGIGGGDFGLKRCLEMASRPATAQNWRKCKRLIIDEISMVDGEFFEKIEAVARHVRKNDKPFGGIQLILCGDFLQLPPVVKRDFKNDNQQTEKRFCSKVLLGKHAFKLIGHVNDEISQRLMKTSKQKIEGNGILATQLCSHTNDANSINESKLDHLPGEKVLFKAEDSDPSSTKHLDSQMTVPSQLYLKIGAQVMLLKNINVSNGLVNGARGVVVRIEKSVPIVRFKNNFEYACKHERWIIKQASGASVIRRQVPLKLAWAFSIHKSQGLTLDCVEMSLGKVFEAGQAYVALSRAKSLDSLRILDFDSKQVWANPQVLQYYKTFRRRLMDTTMTPLGERNRNNSGDKKKISALAKIKKSLMDKPLVSIGRSISLYVDSQAAIKVLESKFKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ37</accession>
    <name>T1GZ37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ37"/>
    <dbReference type="STRING" id="36166.T1GZ37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009129-RA">
        <property type="protein sequence ID" value="MESCA009129-PA"/>
        <property type="gene ID" value="MESCA009129"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888142_0_0_1"/>
    <dbReference type="OMA" id="VIVNECH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF36">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009129-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15423" checksum="B58BABFEAA8C1E7B" modified="2013-10-16" version="1">MSIILKDFKNALFFPSNVRQFISDFSVIIAIFSMSFLDFAVNVATPKLEVPHEFKPTLETRSWIIPPFTNNPIWTIFLAVFPAMLGTILIFMDQQITAVIINRKENKLNKGCGYHLDLFVLAILIEICSIMGLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ56</accession>
    <name>T1GZ56_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ56"/>
    <dbReference type="STRING" id="36166.T1GZ56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009151-RA">
        <property type="protein sequence ID" value="MESCA009151-PA"/>
        <property type="gene ID" value="MESCA009151"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1054820_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588624" evidence="2">
        <location>
            <begin position="24"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009151-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="264" mass="30087" checksum="847324133C71E9EC" modified="2013-10-16" version="1">MMTSKVFVIFTISLLEVFSAAESDYVDEFLGGQDLPQMRLNYPPALRRRPNQFNDMSSIDFDQEFLDQLVDEQARRRFHKHMEALSRQFNYNLNSRLRDRLSFKNPFPSEGEPSTSYNSPDFEYNLPKASSSNGDIVFGLTPSDSRYKVDEEVESYDNDDFKPQQQHSASPDVAENEQQVLFKFINPQKINNFKTQSIFNPGSVGQDIKTVPHLQRPLAENNNFVMKESMGLESDTNVYVVALIAGVTAAVTVGLIVLVVAWYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H098</accession>
    <name>T1H098_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H098"/>
    <dbReference type="STRING" id="36166.T1H098"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009571-RA">
        <property type="protein sequence ID" value="MESCA009571-PA"/>
        <property type="gene ID" value="MESCA009571"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044998_2_1_1"/>
    <dbReference type="OMA" id="TINALNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="95"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009571-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="18129" checksum="E1144C3B41B1254B" modified="2013-10-16" version="1">MTCHSTNMILALQSKLAVMSTDFKQILEIRTENLKIQKSRHDQFTQNSNNYGNKMHVTSLLLEDNNHPSLAPSEQSPLYPKPDQTQLMIYSEVSDNYVEQRAETMQNIESTIVELGGIFQQLAHMVKEQEEIVERIDTNIADAEMNIDAAHSEILKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0G1</accession>
    <name>T1H0G1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0G1"/>
    <dbReference type="STRING" id="36166.T1H0G1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009639-RA">
        <property type="protein sequence ID" value="MESCA009639-PA"/>
        <property type="gene ID" value="MESCA009639"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253110_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009639-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12228" checksum="1D49701FF1D62EF9" modified="2013-10-16" version="1">MVVLYRMGVRSRHQREFHNHLQTISEFLDRQEEPQTTVDNTPPPDYEAPPDYDEVIKIGFDNDISRNRSCQIQTIEQQPTTSRSRNDEIVSNIEVAISRPGYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0N7</accession>
    <name>T1H0N7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disintegrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02182221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0N7"/>
    <dbReference type="STRING" id="36166.T1H0N7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009718-RA">
        <property type="protein sequence ID" value="MESCA009718-PA"/>
        <property type="gene ID" value="MESCA009718"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431371_0_0_1"/>
    <dbReference type="OMA" id="CEDARDD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000002">
        <property type="entry name" value="disintegrin and metalloproteinase domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049038">
        <property type="entry name" value="ADAM10_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF3">
        <property type="entry name" value="KUZBANIAN-LIKE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21299">
        <property type="entry name" value="ADAM10_Cys-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disintegrin" evidence="2">
        <location>
            <begin position="10"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009718-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="20809" checksum="21947A94BAB43880" modified="2013-10-16" version="1">PKGCFTEPQTSICGNGVVEPGEQCDCGWEEDCKDTCCFPMSRHPRIDEKPCTLTPRAMCSPSQGPCCTTDCKLKFGDKCRDDNGCRDPSFCDGRMPQCPPSVNKPNKTICNKEFVCYMGDCTGSICLAYGLESCQCIPTPDDPKTKSCELCCKQPGEGNPCKSSFEWNEPPFDVPDMFAKPGTPCNDYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H151</accession>
    <name>T1H151_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ERCC1-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02016208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H151"/>
    <dbReference type="STRING" id="36166.T1H151"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009904-RA">
        <property type="protein sequence ID" value="MESCA009904-PA"/>
        <property type="gene ID" value="MESCA009904"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1397802_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="8">
        <location>
            <begin position="98"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009904-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="21686" checksum="88D18A051B3F88DF" modified="2013-10-16" version="1">MFEDDDLDDDLANISILTAPSAPKQSKPNPENAVSSEWFDDTFAVEKESIESKVPQNTLEFPKPPITIKPSEKKLVGPSGVAPVIAAASSKAPNPRSVLVNPKQRLNPALRHVHKVPMEFCDTIKADFVVGRTSCILYLSLKYHALNPEYIFNRLQLLGQQYELRVLLVVVDTPDLATTLLNLTMRELLLKIITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H174</accession>
    <name>T1H174_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H174"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009928-RA">
        <property type="protein sequence ID" value="MESCA009928-PA"/>
        <property type="gene ID" value="MESCA009928"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3089611_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009928-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="5953" checksum="FA0D9104DF3D8C78" modified="2013-10-16" version="1">MTSAKYRGRPVTENTLQDYVNELVKKYIQSNIPQWQVLIIPISGDGWALSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1I5</accession>
    <name>T1H1I5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1I5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010047-RA">
        <property type="protein sequence ID" value="MESCA010047-PA"/>
        <property type="gene ID" value="MESCA010047"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1005727_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010047-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="277" mass="32434" checksum="A494A193841AA43E" modified="2013-10-16" version="1">MFLNLHRELNHQPSMSGAHQQMTMILPLLIVFKLQSLKKLSLENVRRSLKLTVLKKDSVVVQMVRQLHKVHSTRDVQLLRHVRIQNMDVVQMEYHQLKDLRIRVVQNHNVLKHSMDVVLTNTHLLKEMTTKDVQFQQLSHQLLPKKLIPQLLHLVKMIQLLNLEFRNHALSMNLDVALTVRHQLKDLNSRVVMEYMMKRTAIILSTAVVTMEKLQLPLLIRKIVLHVLKSHLDVAQMVPHQLTDTMEKDVVAFMNLDVVKIILLQLVVQALKYHKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1P8</accession>
    <name>T1H1P8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the selenium-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1P8"/>
    <dbReference type="STRING" id="36166.T1H1P8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010115-RA">
        <property type="protein sequence ID" value="MESCA010115-PA"/>
        <property type="gene ID" value="MESCA010115"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226195_0_0_1"/>
    <dbReference type="OMA" id="HCKTICC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0711">Selenium</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010115-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="11628" checksum="7584D8FE4955FC4B" modified="2013-10-16" version="1">MEGSIVHVPHGVIVPMASSDGKKCCKGCGPGYPTPLEAMEKGQREKLLYTITIQPDLNHPLGDYLSTVDVDPESPTYSQVIHRTFTKKTGNELHHSGWNACRVVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1T8</accession>
    <name>T1H1T8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial inner membrane protease subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02179082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1T8"/>
    <dbReference type="STRING" id="36166.T1H1T8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010157-RA">
        <property type="protein sequence ID" value="MESCA010157-PA"/>
        <property type="gene ID" value="MESCA010157"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2984989_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010157-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="58" mass="6457" checksum="F3E072C9DAA580F8" modified="2013-10-16" version="1">IKRVVGLQGELVKTLSYKKEFVRVPEGHCWVEGDHTAGGGLSTELPKKRIPLKLSELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2K5</accession>
    <name>T1H2K5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. RGK family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2K5"/>
    <dbReference type="STRING" id="36166.T1H2K5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010454-RA">
        <property type="protein sequence ID" value="MESCA010454-PA"/>
        <property type="gene ID" value="MESCA010454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2596954_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF1">
        <property type="entry name" value="RAD, GEM_KIR FAMILY MEMBER 3, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9262" checksum="1EA46391D8A62F12" modified="2013-10-16" version="1">MFKQTYNISKLYNKKFHFVNEELAKAHAFLVVYSVIDKSSFQNAEKILTDLQDMDLIRTRPVILVGNKIDLARSRAVSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2N9</accession>
    <name>T1H2N9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2N9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010492-RA">
        <property type="protein sequence ID" value="MESCA010492-PA"/>
        <property type="gene ID" value="MESCA010492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925238_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6788" checksum="AE0D28C3463B503A" modified="2013-10-16" version="1">MSFFSTLPRLFKAVALAYADDINLICQILCLCGRIFPELERAAESVGLQVNGDKKVQASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3B4</accession>
    <name>T1H3B4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3B4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010734-RA">
        <property type="protein sequence ID" value="MESCA010734-PA"/>
        <property type="gene ID" value="MESCA010734"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870147_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010734-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7461" checksum="2B426DD5167E18B2" modified="2013-10-16" version="1">MKNLLFPMDDTANDRQSSTSKESQLITSGREVNDFIKYIAKYSTDGLRCYNLRSQIYEIPFSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3H2</accession>
    <name>T1H3H2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polycomb protein VEFS-Box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3H2"/>
    <dbReference type="STRING" id="36166.T1H3H2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010799-RA">
        <property type="protein sequence ID" value="MESCA010799-PA"/>
        <property type="gene ID" value="MESCA010799"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2612870_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21551">
        <property type="entry name" value="VEFS-box_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019135">
        <property type="entry name" value="Polycomb_protein_VEFS-Box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF0">
        <property type="entry name" value="POLYCOMB PROTEIN SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09733">
        <property type="entry name" value="VEFS-Box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Polycomb protein VEFS-Box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010799-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9265" checksum="75D7226301CEA12E" modified="2013-10-16" version="1">LYHHTETCLPVHPKEIDIDSEGENDPLWLQQKTIQMIDDFTDVNEGEKELIKMWNLHVMKNGYTLPSYRNLFTCTSKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3K8</accession>
    <name>T1H3K8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3K8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010836-RA">
        <property type="protein sequence ID" value="MESCA010836-PA"/>
        <property type="gene ID" value="MESCA010836"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2783235_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010836-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7562" checksum="E2D50463B6272030" modified="2013-10-16" version="1">MSIKPLTTPNLNITKFFDNNGKVGVSTSIVGAQYHQLNGDEINGKIKQELELDVDNDLESPSPRPNGDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H409</accession>
    <name>T1H409_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H409"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011007-RA">
        <property type="protein sequence ID" value="MESCA011007-PA"/>
        <property type="gene ID" value="MESCA011007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2628955_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9171" checksum="B48275E6B8D13765" modified="2013-10-16" version="1">MVSTSKRCSKLRTRRFQWAGHVQKMEENISARKCFSTNPIGYRKKEDLKRGVTSYQVMLEELLSPTSEQQQKTVKYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H436</accession>
    <name>T1H436_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3BP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02059388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H436"/>
    <dbReference type="STRING" id="36166.T1H436"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011037-RA">
        <property type="protein sequence ID" value="MESCA011037-PA"/>
        <property type="gene ID" value="MESCA011037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1351887_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007940">
        <property type="entry name" value="SH3BP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423:SF12">
        <property type="entry name" value="LD13933P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05276">
        <property type="entry name" value="SH3BP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="6"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="23105" checksum="E1E83C0053202515" modified="2013-10-16" version="1">PYYETRANYNGLLKVQREKIVNLENKVSESKMTYNEALKKLEQISEEIHKMRQDRKNFFNMLDEPSTHEREFPQKINSIDEYYDVPLKLSTKSSPIHQKSLDSHECPHLLQDFEDSLQISSSTGGGHQTSSGIMSADDIDQWTEIRLTNSDSTSSGYSHNDDQTNLSVSDSNSSYSDDQVQRRRFTCVGLKNPLKCAISGAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H483</accession>
    <name>T1H483_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H483"/>
    <dbReference type="STRING" id="36166.T1H483"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011087-RA">
        <property type="protein sequence ID" value="MESCA011087-PA"/>
        <property type="gene ID" value="MESCA011087"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118859_0_0_1"/>
    <dbReference type="OMA" id="TICEKYD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF13">
        <property type="entry name" value="LP10922P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011087-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15884" checksum="0BA4949203738EB3" modified="2013-10-16" version="1">MYGMSLARYKLCPDVKTKGIFGMKPLVLFTSEESHYSFKKDAHWLGIGTDNCIAVKTNNQGQMSPLDLEDKVIRALAEDKQPFFVNATAGSTVLGAFDDLNAIADVCEKYKLWLHVDAAHGGSVMLSKNHKHLLKGVERTISLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H494</accession>
    <name>T1H494_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139248">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H494"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011099-RA">
        <property type="protein sequence ID" value="MESCA011099-PA"/>
        <property type="gene ID" value="MESCA011099"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1002174_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011099-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="30751" checksum="3B2EA2FF0B4A6E79" modified="2013-10-16" version="1">MVEDMETELSEISKENNIVNESGNGSESNCMEICEESNIKDGSNIKELDSTSSEQQSLLSLTQESENHKDNVEDMDTVEINKESTDTELKPSNIESMEVEAESPSVPKIISVITIHPPSDVDDCFDSPDLIEPKSLTITNAIERNEDYCGRQYQYSGTENCGSRSPSPMPLSAMKTIVVVNTSIQERKIVSKLLTLRKIELDEQGHKTNKTLPIANQQLDSRENGVYCPLRSLLNSSSSSPIISLSTPFFHTAIATSYRRMSTCLKHIIEQAVSGGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4A8</accession>
    <name>T1H4A8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4A8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011116-RA">
        <property type="protein sequence ID" value="MESCA011116-PA"/>
        <property type="gene ID" value="MESCA011116"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011116-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7145" checksum="783403F439CAB7A0" modified="2013-10-16" version="1">MKLVLGDFNSKVGEEDIYKGTIGTDIYSELPQSVCLMFTHYNHVVIIRINKVLTLEVRLSSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4F9</accession>
    <name>T1H4F9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4F9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011168-RA">
        <property type="protein sequence ID" value="MESCA011168-PA"/>
        <property type="gene ID" value="MESCA011168"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751809_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="16448" checksum="9B3E67966AB3E3D9" modified="2013-10-16" version="1">MSHLSICIHKPGTKSLDLNEIFKICEQSGKITSLIPTSVFLFINFKAISNIDSCVRKLQRAGYSAEESRKNRKMRLKYGVPEIQPSNESCSEDEVESVESDSGKGSTSSFGVKEVESSVTGKCKNSGCLRNTSSVCKRCSDFFCSIDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H575</accession>
    <name>T1H575_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S16</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02138876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H575"/>
    <dbReference type="STRING" id="36166.T1H575"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011450-RA">
        <property type="protein sequence ID" value="MESCA011450-PA"/>
        <property type="gene ID" value="MESCA011450"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2043919_0_0_1"/>
    <dbReference type="OMA" id="EVIKPHM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011450-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="12936" checksum="5C835AE3A8078C33" modified="2013-10-16" version="1">MMILQGIEISSADASSVIKCVCDIAEKGGSAINAGDISDKTATAVAYCKRGRGLLRVNGRPLDNIEPKVLQYKLQEPLLLLGKEKFAGVDIRVRVSGGGIVAQIYAIRQAISKALVAFYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5G0</accession>
    <name>T1H5G0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">adenylate cyclase</fullName>
            <ecNumber evidence="3">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02381022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5G0"/>
    <dbReference type="STRING" id="36166.T1H5G0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011537-RA">
        <property type="protein sequence ID" value="MESCA011537-PA"/>
        <property type="gene ID" value="MESCA011537"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_977579_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF8">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="13">
        <location>
            <begin position="116"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011537-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="285" mass="32030" checksum="46BF8B28CD7B5CFC" modified="2013-10-16" version="1">MRNSLAVITGMCFVGIAIGQLTSYKPMKVDLFANASRNLSEFTTEEMKWFIVFLNREFEIGYRLTFYGNAVANKDKMQVQNLKNQADMLLHNIIPKHVAEELRKQAKYSENHHNVGIIFASIVNFNEMYDESYLGGKEYLRVLNELIGDFDELLSRSFEASMTTGNFGIHFVQHLLLQLGLGLGSLLLHFEPSPPPLFPMQQISASSESRFFGRDLAVTIIFGGVLIFLGSSFSFRFKDDTLGRDERMGEGFKEAEMAPTGDAGGTIGWITDKFVALCDSVTPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5I0</accession>
    <name>T1H5I0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5I0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011557-RA">
        <property type="protein sequence ID" value="MESCA011557-PA"/>
        <property type="gene ID" value="MESCA011557"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533966_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011557-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9618" checksum="F494830F20577CF8" modified="2013-10-16" version="1">MSWELDLLSEAIPSLHELLNRDKNRVTPQEEDSLPFDNTEKLWKKLKPALEEVETAIHNLKNNKSAGSDGIKDGQKPYLDYLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5L9</accession>
    <name>T1H5L9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha-endosulfine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5L9"/>
    <dbReference type="STRING" id="36166.T1H5L9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012002-RA">
        <property type="protein sequence ID" value="MESCA012002-PA"/>
        <property type="gene ID" value="MESCA012002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125025_3_0_1"/>
    <dbReference type="OMA" id="SKYPGGM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="3 4" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11089" checksum="922E0575E4BCF7D7" modified="2013-10-16" version="1">ENPRELEKIEEEKLKAKYPVTGLRGPGGHSAFLQKRLQKGQKYFDSGDYQMAKQKGGGIKQVFANKVPTGEAIPSAIPTPETVPARKTSIIQPCNKFTTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5P6</accession>
    <name>T1H5P6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prefoldin subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5P6"/>
    <dbReference type="STRING" id="36166.T1H5P6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012029-RA">
        <property type="protein sequence ID" value="MESCA012029-PA"/>
        <property type="gene ID" value="MESCA012029"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113004_1_0_1"/>
    <dbReference type="OMA" id="CYETERK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012029-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12753" checksum="ED811FE86700E4C1" modified="2013-10-16" version="1">MQQKQLRLKEEIIAEFQNMRNEQRMLATKLNTLEMDLKEHKTVIELLKTVDENRKCFRLIGGVLCERTVKEVLPQLVENKDLMEQAAQKLTENLQTIEQINKYKEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Q8</accession>
    <name>T1H5Q8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone chaperone asf1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ASF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5Q8"/>
    <dbReference type="STRING" id="36166.T1H5Q8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012041-RA">
        <property type="protein sequence ID" value="MESCA012041-PA"/>
        <property type="gene ID" value="MESCA012041"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060354_1_1_1"/>
    <dbReference type="OMA" id="DYADQEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012041-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17691" checksum="547C87E41EF608C3" modified="2013-10-16" version="1">MAKVHITNVVVLDNPSSFFNPFQFELTFECIEELKEDLEWKMIYVGSAESEEHDQILDTIYVGPVPEGRHIFVFQADPPDVSKIPEADAVGVTIVLLTCSYRNQEFVRVGYFINNDYSDPELRENPPPKPLFDKLTRNILATKPRVTRFKINW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6H9</accession>
    <name>T1H6H9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6H9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012312-RA">
        <property type="protein sequence ID" value="MESCA012312-PA"/>
        <property type="gene ID" value="MESCA012312"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138769_0_0_1"/>
    <dbReference type="OMA" id="RRMARYC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000098">
        <property type="entry name" value="Transcription factor LBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051892">
        <property type="entry name" value="LBX_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336:SF8">
        <property type="entry name" value="LADYBIRD EARLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336">
        <property type="entry name" value="TRANSCRIPTION FACTOR LBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012312-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="13093" checksum="8221CF0B4C5A7467" modified="2013-10-16" version="1">SFFADDSHMNLFSTRPQQKKKRKSRTAFTNHQIFGLEKRFLYQKYLSPVDRDEIAASLGLTGAQVITWFQNRRAKLKRDIEELKKDVESVKMMSGHSASFLENLNELNILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6K1</accession>
    <name>T1H6K1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EnsemblMetazoa" id="MESCA012334-RA">
        <property type="protein sequence ID" value="MESCA012334-PA"/>
        <property type="gene ID" value="MESCA012334"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080253_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012334-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="25018" checksum="F364A2B179EC71AB" modified="2013-10-16" version="1">MKIRESVETHQSVVLSLLATIGLLTKFIDVCPPGASDLTKFLSAAKSTELFGSLSMLYSAVVPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEIVSVESLSLKLLDVITILLKYCGNQCNNSENSETQAVIVDLIATIGFFCANNKGNQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFLDEYSKSEKAKKNRLISVLNPDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6L1</accession>
    <name>T1H6L1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6L1"/>
    <dbReference type="STRING" id="36166.T1H6L1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012344-RA">
        <property type="protein sequence ID" value="MESCA012344-PA"/>
        <property type="gene ID" value="MESCA012344"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123539_1_1_1"/>
    <dbReference type="OMA" id="FCMEPVD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052804">
        <property type="entry name" value="UEC_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359">
        <property type="entry name" value="GEO07743P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359:SF2">
        <property type="entry name" value="GEO07743P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="38"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012344-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10139" checksum="BD6776D00ECC418B" modified="2013-10-16" version="1">LTAEEQIKIAKRLVLIQHLPIGTFAFGGPVGAEELRECVICMIEFVTGDQIRYLPCLHIYHMNCIDDWLIRSLCCPSCMEPVDAALLVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H754</accession>
    <name>T1H754_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H754"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012536-RA">
        <property type="protein sequence ID" value="MESCA012536-PA"/>
        <property type="gene ID" value="MESCA012536"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954221_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF520">
        <property type="entry name" value="ALX HOMEOBOX PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="3"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012536-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14809" checksum="9B768F4B1F4F56E7" modified="2013-10-16" version="1">VWFQNRRAKYRKQEKQLQKALAPSVIPSCNGMMRNIQGYSVSRGYQPYPHHNSINRYPQDLFQMGASTYPGMGQPFSMTHGTNMSSVXXXXXXXXMSAEDEWYNKSLSALRMNTSHHPNLSAPMLQYQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9X6</accession>
    <name>T1G9X6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159546">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9X6"/>
    <dbReference type="STRING" id="36166.T1G9X6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000018-RA">
        <property type="protein sequence ID" value="MESCA000018-PA"/>
        <property type="gene ID" value="MESCA000018"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173849_0_0_1"/>
    <dbReference type="OMA" id="QDWKISK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052615">
        <property type="entry name" value="FGFRL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890:SF10">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000018-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12500" checksum="79E638D2CCE7740B" modified="2013-10-16" version="1">MINYGVTVQEVVYMYENNIFFSLKQPEAQAALVSLVEREGHEALITQVIAETAADDEKVMASTVGFNAFIKMIETCDVSVEETILKFAREDFITQDWKISKEVRLLTAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC11</accession>
    <name>T1GC11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02087869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000806-RA">
        <property type="protein sequence ID" value="MESCA000806-PA"/>
        <property type="gene ID" value="MESCA000806"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266803_0_0_1"/>
    <dbReference type="OMA" id="ICSAQIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577198" evidence="2">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000806-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="9985" checksum="D1549ECF02016ECA" modified="2013-10-16" version="1">MKVSLVILLVFVAICSAQIGYGGGHGGFAGGFGGYRGRYVGGLIGHPAFGPSYKPGYGGYLGRGGGSSSQSQASASSQSFNNGFGGSSSSSSASASSSSSNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC70</accession>
    <name>T1GC70_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000870-RA">
        <property type="protein sequence ID" value="MESCA000870-PA"/>
        <property type="gene ID" value="MESCA000870"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1471785_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000870-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20282" checksum="D7209F3BC8DDD26A" modified="2013-10-16" version="1">AATCLHEGYIRLLTKLSKKLKWPPTINNSLQLRSWILKGLSIMLPPSPSGVNLCGLGEKGTPPGGILSPLVWLLVVNTIRNLSIYTALSMISNWDKSCGLAVNPSKTESLIFTKNRIPSYMDLFDDKVFSLSCHTKYLVVILDPRLSDALKPPMLAGNPSVRIVSFDNAVIRPVLMYGSIVWNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCN1</accession>
    <name>T1GCN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxisomal membrane protein 11C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02032882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032887">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCN1"/>
    <dbReference type="STRING" id="36166.T1GCN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001051-RA">
        <property type="protein sequence ID" value="MESCA001051-PA"/>
        <property type="gene ID" value="MESCA001051"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100620_1_0_1"/>
    <dbReference type="OMA" id="PIEKICW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026510">
        <property type="entry name" value="PEX11C_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20990">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20990:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23384" checksum="266B8755CD7AEA1A" modified="2013-10-16" version="1">MKGLCYSAKLVSGLQKTRNPEWSRKFGIISSKISGARATLRLIDDIPMIKYALDYGLGFQEKDAVMASLGITANVVDLLYYPIEKICWLAEHKVMNVEDPDKWDILSSWFWVASIYLNLMRLTRNFTKLEEKRRLMNERSEELIKKQRLEVISMIRGGLDLVHAVSTLPKGYLWGGKLETWHVGLIGTTSAALGLYQIFLKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCN5</accession>
    <name>T1GCN5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121554">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001055-RA">
        <property type="protein sequence ID" value="MESCA001055-PA"/>
        <property type="gene ID" value="MESCA001055"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906665_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001055-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7139" checksum="47438EAE8DFF5F95" modified="2013-10-16" version="1">MANVYFENFEQRLDVHATGTILGCTSFSTHPSRKSRPNFLEFRLGSQQEKWMNLIAVCDTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD55</accession>
    <name>T1GD55_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001236-RA">
        <property type="protein sequence ID" value="MESCA001236-PA"/>
        <property type="gene ID" value="MESCA001236"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888139_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001236-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15429" checksum="C87F0BCBBC054654" modified="2013-10-16" version="1">MEEEMHKERNTSIICPIHKIVTSLHPRKLVAISSSKIFLLVSAHSSSCNLIVLIKILIPDVESQNDCHEASFCFLIITIIRTSLENLYLETLAFQPYITLMKSHGNKKIREKWENTEAVATLHNPLLYFKIIVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDC2</accession>
    <name>T1GDC2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDC2"/>
    <dbReference type="STRING" id="36166.T1GDC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001306-RA">
        <property type="protein sequence ID" value="MESCA001306-PA"/>
        <property type="gene ID" value="MESCA001306"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_630536_0_0_1"/>
    <dbReference type="OMA" id="CPICCAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001306-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="435" mass="50211" checksum="E71C122C87F66B19" modified="2013-10-16" version="1">MLIIFSHFPHTSDAVVHYIKTKAPNSDITTVASTIYEFVKQYKLRWKKSNFRLEYFKKHNFSWLSKVLIIEDKLVLNTESELNIDTINNNFALPNSNMPSTSNYRVLDQIPSVEEIQFSQDLLPSYKKITEAKNRACPPIVSSEQGCWVEIQTMLDETTARLFQSLSPGSYELIWKMGMDSTSGLNSFHQKSNEEINESTLLTISLVPLVLEYMTKQIMWFNPVCGSTRFCRPLEISYTKETKPTIKEKYDFYQSKIKELSSTVIKDSHIKHKIFWTMLDGKCVNCITNVDDKSCSTCGSPISHIQDTNRSFIPVDDNLKFGIPILHSKLNTLRNILEVAYKLPFSDDYRKMQWLKKEAKTKLLKDLDEKVKDNKNRGSNWFKVDKIVQGKGTSNTGNVARKFFSQYEKISEITGKMQVELTAVLPSLYELINIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDK5</accession>
    <name>T1GDK5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001394-RA">
        <property type="protein sequence ID" value="MESCA001394-PA"/>
        <property type="gene ID" value="MESCA001394"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742958_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001394-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8506" checksum="D55ED41A680E81F2" modified="2013-10-16" version="1">METVRLRTEAQVDETCTTAATLRTTSLEFVLLFCVTRWKPINERLCNMYAPYKSRMFLHYHNLRSCFQGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDX6</accession>
    <name>T1GDX6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001528-RA">
        <property type="protein sequence ID" value="MESCA001528-PA"/>
        <property type="gene ID" value="MESCA001528"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1671329_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001528-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="17858" checksum="E07855A6875AF5A8" modified="2013-10-16" version="1">MLLSFILRIHITGPVGSVGGTIKFLFSSIYGSIEGDFGGHQSIYCQNNSSVDFNSYFVIGCNFQPLANKVFDYRDVIGTHGDDWLYCGELRVESMYIVLAPFTCRPTDSAARSKCRKKLQENVLTYRNPLIQEPLNYYPVFVSEIQIVLRRPGKVNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEK0</accession>
    <name>T1GEK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02038298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001765-RA">
        <property type="protein sequence ID" value="MESCA001765-PA"/>
        <property type="gene ID" value="MESCA001765"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1300864_0_0_1"/>
    <dbReference type="OMA" id="VAYTTAH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001765-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="184" mass="18856" checksum="B248A4D2BC12193C" modified="2013-10-16" version="1">MNRFPSKQRVEPSSLVEKKQEKRGIYYNGLSGAGLGGTGLGYEGLGVNGLGYSGYSGLGGVGGYNSVGGYNSLGGYNSLGGYNSLGGYNSLGYQPGTVGLVGLGGVGSSFGGLGTSGYYNPYYNSRVAGLGGLSGGYNGYTNGYYNGYSRNAYSPYNRGYSYNSYPSTIGSGYNYGPGISGSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEQ4</accession>
    <name>T1GEQ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF243 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02062493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEQ4"/>
    <dbReference type="STRING" id="36166.T1GEQ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001825-RA">
        <property type="protein sequence ID" value="MESCA001825-PA"/>
        <property type="gene ID" value="MESCA001825"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098085_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576867" description="DUF243 domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="69"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001825-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="22612" checksum="F4B66F044BD1F785" modified="2013-10-16" version="1">MHKLSIITLCLISVSSASPVPDIGLQAQGANDYNIPPMMTSALEDSYSNAAAAGYDDSQGGYDLPQMKPIVHKHIYVHVPPPSIEQEEEQPRIKSRPVTPQKHYKIIFIKAPSPSISPILPPVQNHEEKTLIYVLHKKPEIGDIVVPTPAPTQPSKPEVYFIKYKTKKEEKPVYGPPPSEVMHEKSALDAIIPEDIPLQYGAPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEW5</accession>
    <name>T1GEW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001888-RA">
        <property type="protein sequence ID" value="MESCA001888-PA"/>
        <property type="gene ID" value="MESCA001888"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815353_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001888-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="8036" checksum="7B260A30E6EDCCB8" modified="2013-10-16" version="1">MARLFNQLINGTDSRVPSIRTHFSFPQFRKRPLLTILEKESITYKIREEYRKIHRKLLINVTFSSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF12</accession>
    <name>T1GF12_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02055123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001940-RA">
        <property type="protein sequence ID" value="MESCA001940-PA"/>
        <property type="gene ID" value="MESCA001940"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834055_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001940-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7607" checksum="A8C3AFD554DBC012" modified="2013-10-16" version="1">MFFLFGYLPVDQFLKIGGKGCCEIGEASNSKRSLFLHKETIYYTMDKKHKSTPQNTDFSDIQLIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFH3</accession>
    <name>T1GFH3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02079076">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFH3"/>
    <dbReference type="STRING" id="36166.T1GFH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002115-RA">
        <property type="protein sequence ID" value="MESCA002115-PA"/>
        <property type="gene ID" value="MESCA002115"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1171790_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002115-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="237" mass="26869" checksum="D39FF9B109BB6A2D" modified="2013-10-16" version="1">MFPASSNASIFEANRERPSNVFTQYSSHSSVHGVQYLGDPKRPILESFPPEYLLGSNIKKESNWDLENGYDSSNKSYPLRATGNGFKSGLKTYLSMYKDDNDPMCRFNENGFKVILHAPNEIPMVNEDFILISNQERSMISITPKVIETSEEIKKYDISRNRTTPVCGAIMKECMYKAELSLLDTRTQGDEITCNCLPTCISITYLYETSQATYDMKLKARLGVRTTDSGKLEIFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFN6</accession>
    <name>T1GFN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002181-RA">
        <property type="protein sequence ID" value="MESCA002181-PA"/>
        <property type="gene ID" value="MESCA002181"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_984446_0_0_1"/>
    <dbReference type="OMA" id="CREMEFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577304" description="Fibronectin type-III domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002181-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32056" checksum="038EEE1952CDE617" modified="2013-10-16" version="1">MKMLNLRLIFLKTLLCGLVFGQQQQHQQSQSSGTRDLFKCRQMCYQKFIQEWHQCMDYVDCKSMCITPPNINSYIPSEAFSILCWNNCDDNLGPFTLSVSSAFVQGNLVLTDIAWDQAITNQSKQCLVTWEVSGGGLMGNLLTDSSTVELSLWPNTVYNVEVTCKHKETGGMRRSLKLIVDTSKLIGNSVILHQPNIDINQISSSFPSTSTNDEDRRRILKDSSKDDFSSSYNPKIIQKYSKSQREEVRVNEIHRSKLTTTSSTLFSMEFTTETVILAMVVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFU8</accession>
    <name>T1GFU8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFU8"/>
    <dbReference type="STRING" id="36166.T1GFU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002248-RA">
        <property type="protein sequence ID" value="MESCA002248-PA"/>
        <property type="gene ID" value="MESCA002248"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675416_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002248-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="408" mass="46558" checksum="1674A16943C67A73" modified="2013-10-16" version="1">MSNDLNISIHDMFIPWMRKAKHKHRHRRNSDNESEISHTSGRSGRSGRSGRSGRSHNSYRKRRRSRHRRGDSGSENNSIRTRARSYSGHRVSSSCSVGSTELIDSTNQWLEVQRRQANGEINNGIQQASVSKSSYVVKSLHSNDSYSDSHSYHKSKRHRKHHNSSSKLPSDSKNRIWSSELAKHLQFDLVDTAGMTEDQLREIPYTVVETKSKNKRISSTNFKLSKSLSSGNTSSTFSKECNNGSKEKDRVDRIRGMMSSMSMGDFVSPSISSLSPQENSTLRVSHEHTDSGLGADQDYAYSSERSSDSTRYGTNKSSGTSSGSNNKSNNVPYSSLLSQNKKSVSNTMKTHSVQHYENLKPLQSQMMRHHGQQHLRQHLQHEHEQEQQQYQHQYTKKQQPQQQQRIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG09</accession>
    <name>T1GG09_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002313-RA">
        <property type="protein sequence ID" value="MESCA002313-PA"/>
        <property type="gene ID" value="MESCA002313"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798652_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002313-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16585" checksum="0482F3F15FEF7B49" modified="2013-10-16" version="1">MGDRNQATVLRERKRKGFSTTTSCCKNKNGDLITNKEEVLIQWEDFFKELLNGNESRTSPQGNQSQKFKNNEDAQNSWTSADFKLLSVYDRGVLQLIFGGVCINGEWGSGYNQELYELYNHPDIVKKIRTRRLRCAEVTREPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG40</accession>
    <name>T1GG40_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175625">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002346-RA">
        <property type="protein sequence ID" value="MESCA002346-PA"/>
        <property type="gene ID" value="MESCA002346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560896_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9523" checksum="BB474526FF2BDFE3" modified="2013-10-16" version="1">MSELTFNMFTLKEAHQYPLLNLNVQRIHCIQHLDNKLAILGQTKVCDILTLKLYQSNCERFPFAFDTVRIPSTRPSSKLITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGL8</accession>
    <name>T1GGL8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02062853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="MESCA002541-PA"/>
        <property type="gene ID" value="MESCA002541"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898628_0_0_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576931" evidence="1">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002541-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15110" checksum="5FEB4BB7E3214716" modified="2013-10-16" version="1">MKFLAITTFIILTLKFENVLGQSKCSIGHEVIHMNDGSFYETSLKDYTHNEPKSTEVLRIKAFHIGTGINIQYSGNQRNLYFANHNSSHIALYKNVADYYSKITYCDTAPHSGFLSEHSAAEIDLIITSKCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGN8</accession>
    <name>T1GGN8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005533">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005534">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002561-RA">
        <property type="protein sequence ID" value="MESCA002561-PA"/>
        <property type="gene ID" value="MESCA002561"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560878_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577353" evidence="1">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002561-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9532" checksum="7BD28EAC01CA60D9" modified="2013-10-16" version="1">MVSFICTILLVIFWSSSIKKLLEDSNMTYKGTFKIRDPNINSRNSVTPWQTVNERLNIFGMTTYCKFKDKAEYSIPGKLSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS2</accession>
    <name>T1GGS2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02100906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02100907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGS2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002601-RA">
        <property type="protein sequence ID" value="MESCA002601-PA"/>
        <property type="gene ID" value="MESCA002601"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925247_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002601-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7291" checksum="F8CD2CB4A0DB7CF4" modified="2013-10-16" version="1">MELKGFIDIFKLNSLELTPAPVPKEDFKPFVYTSYYPSRCGMVDIMQYFRDTDDQNNFHNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGV6</accession>
    <name>T1GGV6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002637-RA">
        <property type="protein sequence ID" value="MESCA002637-PA"/>
        <property type="gene ID" value="MESCA002637"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500471_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002637-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9138" checksum="80635E8B7929E479" modified="2013-10-16" version="1">MAAAGINCTPVGEMAVVVVISELVYNYTTTMSCIGSTGRAFRGACTRKYAGDKAIVSGESLTCVNSLRFLKLTELKHFIPAKNTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGZ8</accession>
    <name>T1GGZ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGZ8"/>
    <dbReference type="STRING" id="36166.T1GGZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002680-RA">
        <property type="protein sequence ID" value="MESCA002680-PA"/>
        <property type="gene ID" value="MESCA002680"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173840_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="18"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002680-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12612" checksum="FC85BCCE7B7B94CE" modified="2013-10-16" version="1">MYNILYVKTKLPQRKINAAHCFLYNSGKNVKVRLGSTYLNSGGVVVNVTNITVHPDFNYVSFKYDIAILKLEPAPPPNLLQAHTYLFLDGAQHQRLLIPSQNYFKKLKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHU7</accession>
    <name>T1GHU7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02181903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHU7"/>
    <dbReference type="STRING" id="36166.T1GHU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003009-RA">
        <property type="protein sequence ID" value="MESCA003009-PA"/>
        <property type="gene ID" value="MESCA003009"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085834_1_0_1"/>
    <dbReference type="OMA" id="DLEQNWL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905:SF1">
        <property type="entry name" value="AT07410P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905">
        <property type="entry name" value="N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003009-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="20985" checksum="CB7BC0739DF04A4D" modified="2013-10-16" version="1">MAEIIYRVANESDKEKVGKFLYDHFWPDEPTFKAAGVLPNKEENDDVLKCIDYGTCTIAEDQNGTIAGVRLAKVRVPADIEKPAEKPWTQLDKILNSCSMLICVSRDFRGKGVGLKLYSENMELGKRLGFKAYVCDCTSAFSAKLCEKLGMEVIAAIEYTECLDENGEQLFKPEAIHDKIRMYGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI29</accession>
    <name>T1GI29_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02171159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="T1GI29"/>
    <dbReference type="STRING" id="36166.T1GI29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003096-RA">
        <property type="protein sequence ID" value="MESCA003096-PA"/>
        <property type="gene ID" value="MESCA003096"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_8_1_1"/>
    <dbReference type="OMA" id="FTTMPAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF92">
        <property type="entry name" value="MODIFIER OF MDG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003096-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11501" checksum="8D111287D635A3E4" modified="2013-10-16" version="1">MADDEQFSLCWNNFNTNLSAGFHESLCRGDLVDVTLAAEGQLVKAHRLVLSVCSPYFRKMFTTMPANQHAFIFLKDVSHSALKDLIQFMYCGEVNVKQDALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIA0</accession>
    <name>T1GIA0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003169-RA">
        <property type="protein sequence ID" value="MESCA003169-PA"/>
        <property type="gene ID" value="MESCA003169"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500504_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003169-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="10002" checksum="A47E02C62820FEED" modified="2013-10-16" version="1">MQIEVNVLTKQEAKNSKYEQNILKKYYYQSETPGSNLHNIYFLLRFVSFDCLHEPGRVLLTISKNPGMPNWFIARKSLSLVGVSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIC2</accession>
    <name>T1GIC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIC2"/>
    <dbReference type="STRING" id="36166.T1GIC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003192-RA">
        <property type="protein sequence ID" value="MESCA003192-PA"/>
        <property type="gene ID" value="MESCA003192"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3108801_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003192-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="51" mass="5810" checksum="57539658E953A40C" modified="2013-10-16" version="1">MSQLADKAKSVVRDLDPSNDLTFLRVRSKKHEIMVAPDKDFILIVIQNPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GID3</accession>
    <name>T1GID3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PLD phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GID3"/>
    <dbReference type="STRING" id="36166.T1GID3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003206-RA">
        <property type="protein sequence ID" value="MESCA003206-PA"/>
        <property type="gene ID" value="MESCA003206"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027021_2_0_1"/>
    <dbReference type="OMA" id="DINEERP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09106">
        <property type="entry name" value="PLDc_vPLD3_4_5_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050874">
        <property type="entry name" value="Diverse_PLD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185:SF17">
        <property type="entry name" value="GM01519P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185">
        <property type="entry name" value="PHOSPHOLIPASE D - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003206-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="294" mass="33609" checksum="BF47A75D772F4AE5" modified="2013-10-16" version="1">MFSYVLTKYLFFYISFRWKRSGWCKPSCIPITVILTLIILVVLLPLLEHANDKSSHTKTSGSSFICTDQCRIQLVESIPEGLNYSHDSPKFMSTFEAWNILLDITNESLDIGSFYWTLRGSDIFNHTSAQQGEKIFQRILNTGIQKKYPIRIAQSAPTPGNPNLDTEYLVKKNAAEVRSVNFPRLIGGGVLHTKIWIADNQHFYIGSANMDWRSLTQVKELGVLVTNCTCLAKDMSKIFKAYWFLGKNNSHIPAHWPDEYSTKFNMNSPITVNYNEEFNLNTFITVSIPPPQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIK0</accession>
    <name>T1GIK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003280-RA">
        <property type="protein sequence ID" value="MESCA003280-PA"/>
        <property type="gene ID" value="MESCA003280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123884_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12656" checksum="830A5A5245D4BDFB" modified="2013-10-16" version="1">MYSTIQPASRYLNMLMENLLGNLSLAITAFVKYIAFKSEISSLLRAFTTTPSVTDNVSGVFGPSKAEVPISSFIITERNLAPRAKVLSSVFDVHKICLTYSPFFDFPQLKCAYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIL2</accession>
    <name>T1GIL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 58</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIL2"/>
    <dbReference type="STRING" id="36166.T1GIL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003293-RA">
        <property type="protein sequence ID" value="MESCA003293-PA"/>
        <property type="gene ID" value="MESCA003293"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_724216_0_0_1"/>
    <dbReference type="OMA" id="MSEWTSK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003293-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43637" checksum="11AA675DFDD21B13" modified="2013-10-16" version="1">MGGNLLTEVPDTVGNLHQLQALVLCDNLISSLPTSIARCKNLKSLLLHKNRLRHLPKDIVALQNLTEVIEFERKSTSRPFRSGHLSSPPSLMELSGRMVKSANVPYGPRDLPRSVIEYLNCAKCCVNPNCQGVYFDNRVEHIKFVDFCGKYRVPLLQYLCSSKCIKERPNSSVEPQPGASGYMMRKTKQKSIKMMEVYTSDSSDTDSRELKTLSFSRENLTYIRLDDEFVHYAQTNSKSLGDIETILLNHNRLIRIPINIKQFYNLRTLDLSSNNLGELPEAIFQLGLVTLIAKNNHLTNDSLPKSLLPSKNTMSTLKELNLSGNMMTEFPEQCLDLHFLKYLYIGGNKIRSIPKDVWKMTRLVFVFDTIEWGNYLYNIYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIR9</accession>
    <name>T1GIR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02092816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIR9"/>
    <dbReference type="STRING" id="36166.T1GIR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003354-RA">
        <property type="protein sequence ID" value="MESCA003354-PA"/>
        <property type="gene ID" value="MESCA003354"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023330_0_1_1"/>
    <dbReference type="OMA" id="KGVWETR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19954">
        <property type="entry name" value="serpin42Dd-like_insects"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588338" description="Serpin domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="6">
        <location>
            <begin position="27"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003354-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="367" mass="41214" checksum="207BD497ABAD6010" modified="2013-10-16" version="1">MKIFLKLGILVVMSLSAYSSEEPLFATDLYKAIGENGNMVFSPYSIQACLGLAYMGAGGQTASEMAKGLRFGSMTKDQVADSFSKVLKDSQSSNMLKIANKIYVNKGSQINFAKSIESEKVINDWVEKETNGKIKDLLSPGTIDQQTKAILVNAIYFKGHWESPFPPKSTRKLDFYSSSSKAVQADFMYDDDRFLYGSLDDLDATAVELKYKDSPYSMVFVLPNEKDGLPKLVKKIGNNYDLLSVSKKLSKQKIYIYLPKFEVEYDIELKPALSKLGMPSMFSRKADFNSMFKDSESIYISKAFHKAMIRVDEDGTEAAAATCKGTNLKAYPMSMFLGQKTFKADHPFLWYIKDESNIYFMGQVTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIV3</accession>
    <name>T1GIV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02193684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIV3"/>
    <dbReference type="STRING" id="36166.T1GIV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003388-RA">
        <property type="protein sequence ID" value="MESCA003388-PA"/>
        <property type="gene ID" value="MESCA003388"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_998496_0_0_1"/>
    <dbReference type="OMA" id="EACHVIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003388-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="279" mass="30442" checksum="EA86497C9601F91F" modified="2013-10-16" version="1">MCILQYRFILYSSLSSFYIPCIMMVFLYWSIFKALRNRARKQKAAKRPHLSEITCGSVIENVAQTRRYAETTLDSSKSVSRIVPDEPATNTGSGSNEDEDENAISPEEIEACHVIVNDKSTEFMLATVVEETGKKFPSSVVAQISSNPQLVVSSDANGNHDSGYVPSNIGDSVVIANPSISSPPQSPEPSGSKSIAPSSTASVSAGNTNNNKQPQQQLKGKEVDNSSESPKKDSLSMAMKPLSYHVKYETPDTLDRNDSTMSAFSKTSRKDKRNSQASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIX5</accession>
    <name>T1GIX5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003412-RA">
        <property type="protein sequence ID" value="MESCA003412-PA"/>
        <property type="gene ID" value="MESCA003412"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576591_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003412-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9406" checksum="FBC6A48F4B2B75A3" modified="2013-10-16" version="1">MLLFILPYVIDVFTAAINRFFEESTLPKNWKQAKVIAVPKKLEYANNVFDRIVYNITIWTTPSKKCITKIQTTLSTSLPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJU8</accession>
    <name>T1GJU8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02153944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJU8"/>
    <dbReference type="STRING" id="36166.T1GJU8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003752-RA">
        <property type="protein sequence ID" value="MESCA003752-PA"/>
        <property type="gene ID" value="MESCA003752"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1742637_0_0_1"/>
    <dbReference type="OMA" id="HEPPRSW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576981" description="Palmitoyltransferase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="37"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003752-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="17706" checksum="6480341EE83E9EAD" modified="2013-10-16" version="1">MAFFLLLNLSTNWIFCIVTDTSNNDIEPPLDPVELSSWRFCSDCKKHEPPRSWHCKICQSCILKRDHHCMYTGCCIGHWNHRYFLMLLVYMTYSSTYVTVLTFKYIWSYKYDEFFNLNTIFKLFCPVSMLVLDSASFLPSCFLLPTCLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKG6</accession>
    <name>T1GKG6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GED domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093346">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKG6"/>
    <dbReference type="STRING" id="36166.T1GKG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003989-RA">
        <property type="protein sequence ID" value="MESCA003989-PA"/>
        <property type="gene ID" value="MESCA003989"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3160501_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GED" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003989-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="48" mass="5645" checksum="A514AA4436A42775" modified="2013-10-16" version="1">MHFLVNYVKDNLQSELVTHLYKSDSEDLLNESDHIAIRRKEAADMLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLJ5</accession>
    <name>T1GLJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component Sec3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02122769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLJ5"/>
    <dbReference type="STRING" id="36166.T1GLJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004400-RA">
        <property type="protein sequence ID" value="MESCA004400-PA"/>
        <property type="gene ID" value="MESCA004400"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015381_0_0_1"/>
    <dbReference type="OMA" id="REIWANN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588380" description="Exocyst complex component Sec3 C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec3 C-terminal" evidence="3">
        <location>
            <begin position="142"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec3 C-terminal" evidence="3">
        <location>
            <begin position="337"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004400-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="450" mass="52052" checksum="90C84D70B0A7EE96" modified="2013-10-16" version="1">MNRICRIVFCSCHFILKLFNLCKSNSTGALSNISRIRASREDLLDTSTSRKASAVKVTVPYGTLGASREIWANNVESAERDKFDATLEKVLAELEPVALQEQMFCINFFQLDVLSPTGKNTQTTLDPEEKPTSSETDPNISEYLNSSQFPQKKIDRQINEEVRKMMIALFGILEAELINFIQSFEKLDSFYTLYVLVRLTQHVMSAQDTHSFLSMTFASALVHVKRNFDRFMQQQVQSIVECKLPKKSKCGILPYVENFEDFASTAENIFKKTERRADMDKWYLQIVNKIFEYISVHSNDHPKTPSQVIKMENYHHMFSLLSQLKVPGLDAAKKDLFFEGVQQKVAQGVKETEISYQMAFSKQELRKVISQYPAREVKKGLENLYKKVEKHLCEEENLLQVVWRAMQEEFIAQYNYLEERIQKCYAGAMITLEFNINDILNFFSDIAKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLK8</accession>
    <name>T1GLK8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLK8"/>
    <dbReference type="STRING" id="36166.T1GLK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004415-RA">
        <property type="protein sequence ID" value="MESCA004415-PA"/>
        <property type="gene ID" value="MESCA004415"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053597_4_0_1"/>
    <dbReference type="OMA" id="NGHECML"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398:SF21">
        <property type="entry name" value="AGAP004005-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398">
        <property type="entry name" value="AGAP007094-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00718">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004415-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="21357" checksum="434DDAEB8E89AEE3" modified="2013-10-16" version="1">LAIRDKRWLIFPEQDPQRHQFVIGVGIPLEDLEFEALTTGYVLKAEYFLPINESDFRKDPFVIHFIEMNAENQQKRSLDSPMSRFDIYKSFEMLLNRRGLDGKQCLMVAICEAGSISFQEGFDTLGDIFHLFLTPSSTEDEISQSVDNDYIQAEIFGRAGGNCVKRFNRCNLSIMVSFHLLFKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLS3</accession>
    <name>T1GLS3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLS3"/>
    <dbReference type="STRING" id="36166.T1GLS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004486-RA">
        <property type="protein sequence ID" value="MESCA004486-PA"/>
        <property type="gene ID" value="MESCA004486"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476788_0_0_1"/>
    <dbReference type="OMA" id="MTINRMS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004486-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20348" checksum="D3EE6EAAB47FC37D" modified="2013-10-16" version="1">MAFGTPSTTSEPMIIWSMEPTQPPVNRRTKMPNMKAPIMSNDDTLIIGIVGGVVAFIAILIIVICIVRLRMTSMDYPPPGPMMGMPAMPLGPGSVQMSYKGGPPTPLYAVQNYATLPHKGIHHSQQNLSSQRQPSYMTINRMSYFGNGQQQHTLPPPSQQQQSVQSQQHNQPYMIYSDEKAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLU0</accession>
    <name>T1GLU0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heat shock protein 83</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">HSP 82</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLU0"/>
    <dbReference type="STRING" id="36166.T1GLU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004504-RA">
        <property type="protein sequence ID" value="MESCA004504-PA"/>
        <property type="gene ID" value="MESCA004504"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006684_1_3_1"/>
    <dbReference type="OMA" id="HMERLMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000003">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="8">
        <location>
            <begin position="12"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="109"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004504-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="528" mass="60806" checksum="0A6400170E376B23" modified="2013-10-16" version="1">MMKLIVNSLYRNKEIFLRELISNASDALDKIRLLSLTSKEELNTNPDLNIKIKMDKDNKILHIMDSGIGMSKDDLINNLGTIAKSGTADFLSKMQDPDSTSDLSDMIGQFGVGFYSAFLVADRVIVTSKHNSDEQYVWESDANSFSIIKDPRGDTLKRGSIISLKLKDEASDFLQEDTVKNLIQKYSQFINFPIYLWSSTEVEVDEEVEEADSKPSSDETDDEANIWTKSDNIKLFVRRVFITDEFNDIMPNFLNFIRGIVDSDDLPLNVSRETLQQHKLIKVLKKKIVRKVLDMIGKIDKDAYKIFWKEYSTNIKLGLMEDAGNRARLAKLLRFQSSSNDFTSLTEYVERAKENQEHIYYIAGSSKAEVTKSPFVERLLKNGYEVLYLTEAVDEYCISSLPEFDGKKFQNVAKEGFKLPEETSNIEELKSTFEPLTKWLFNVALKDLVLKVEISERLYSSPAALVAGMFSWTGNMERLAMSNAHQKSDDSQRSYYLNQKKIFEINPHHQVIRELLRRMKVMKMIHWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW6</accession>
    <name>T1GLW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004531-RA">
        <property type="protein sequence ID" value="MESCA004531-PA"/>
        <property type="gene ID" value="MESCA004531"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1405727_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004531-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="22840" checksum="FAF51891A4318CD9" modified="2013-10-16" version="1">MTQQIYLNFGKGYHMDMSQHPHYQQAQHQSHIYINSGDKRTSHIQSQQSQQQSQHSSSPSAERGHSSNQHHHPQPPQHHSHHHQQQQQQQQQQQQQQKSMPSPKPVWSYDKNQKIQGFHRMEYANESIDMTRTPPLPPQQPPSPRWRKSQPPPPNQGPMPMTYKYPMSMDDRYIEMMVQDGRSSPTPRFITMPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM62</accession>
    <name>T1GM62_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM62"/>
    <dbReference type="STRING" id="36166.T1GM62"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004630-RA">
        <property type="protein sequence ID" value="MESCA004630-PA"/>
        <property type="gene ID" value="MESCA004630"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1706286_0_0_1"/>
    <dbReference type="OMA" id="IMYEHER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004630-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17451" checksum="4BC18679132FB5CB" modified="2013-10-16" version="1">MDELKYFTDIQSDGVQGVPLDNYGNWQGCILGPPGSPYEGGKFFVYLYFSELYPFTPPTLRFLTKIFHPNVSRHGDVGIDTIQHNWSVSLTLSKLLLSVQSLLTDPYTEICMEPILGIMYEHERDRFSELLGNGQKSMRCLNGCHPVKLIGPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNJ3</accession>
    <name>T1GNJ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005147-RA">
        <property type="protein sequence ID" value="MESCA005147-PA"/>
        <property type="gene ID" value="MESCA005147"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796869_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005147-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7503" checksum="7436A71CBDA10927" modified="2013-10-16" version="1">MTNHGHSGEVQTRTGVIGANSHRLEIQKKHIACKQMSVQPDQTTTILTVVPQNQSYGSESWNLTSVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNV6</accession>
    <name>T1GNV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02145466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNV6"/>
    <dbReference type="STRING" id="36166.T1GNV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005267-RA">
        <property type="protein sequence ID" value="MESCA005267-PA"/>
        <property type="gene ID" value="MESCA005267"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002633_2_0_1"/>
    <dbReference type="OMA" id="DFQHHQI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000014">
        <property type="entry name" value="Putative regulator of nonsense transcripts 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000092">
        <property type="entry name" value="SD07232p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="59"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="426"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="630"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="849"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="898"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005267-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1108" mass="127913" checksum="58FA8F76D5F0E076" modified="2013-10-16" version="1">MADEEPSQVEDVTAKEKEELQTFINELQAKIESKTNLRRENMNCVRPSEDYFSKLDSSLKKNTAFVKKLKQFTSAQLDTLIKDANKICQALVEAKLKLTDVASIIILCSKLHQTYADFNVQFFEAWQRNFATKPGEKLSNSSKLRVDIRLFAELVSSGVINQKQGLNLLGNVLTHLISQDKEEHSSYNIILSFCKHCGEEYAGLVSQKYHDLAVKYEVQMPKSNFLPPEKQQALRGLLKEYFKTLSTHLLSEHQEIQSISRGIRKALESKGEVSDERKEKCELMQQQFDKFLTSAQTLSDLINEPLPILPKDKEEISGTVLDSISEGDLDPWRGDEETKSFYCDLPDLRQFLPNYAPKQKDIQLEEPSITEEQLDGEIDTDQLDVDDPPSSSSDVPNEAETDNPLQKKILLLLQHHLSGNNKEAFEQFLRHLNNCVNTELIDSAAIEFLLNFNTKNNRKKVGNYIFSVQRTRLDLLPFFSRFVAIINLVHADIANDLAHKLKMEFKWHIKRKNQLNIESKIKIVRFIGEMLKFGLYQKLEALYCLKILLQDFQHHQIEMACALIEVAGVYLYNCKDTRLRMNVFMEQMIRLKTNTHLDSRHSAQVENAYYLVKPPEVGPQEKKTRPIIHEYIRYLIFEELCKQNVDKSIKLMRRLNWDDPEILADLVSGIVSYQEKAIQKVIDNVFEDIRAGLEIHSVKLSQRRLAMAKYLGELYNYRLIESSDVLNTLYSIISLGVSFEEGVESPLDPTDSTFRLKLACVLLETSGQYFTSVLSRKRLDYFLVFFQHYYWHKKNLRAFSDYLLPRIVEDMYKDCLAVVRPKLKLFKSIEEAREAIDKLRRELYPTLFSQPSSRDGAGDTESEGNLETIREDSENDDMTGGETSEALVESDGDIRGNDDEEGEDYDDEYNDKEEESRESPEMLDDIEEMEVVDPAVTQEDLEFEQLKTEGIFKNHRKDIPVPMTSKTIKKSYDQIQSGKIAASTTPSPVPVKDESVPFVLMVRGGKGKQQFKTFEAPSDSALAINLRVQEQKNRDEKEMFKRITLNITERIEEEDYAIEQWKQKQNVAPNRFPNQNKSQKFKHQKGAPDVEAIFECFNGRIKNFTNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP76</accession>
    <name>T1GP76_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02127681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005395-RA">
        <property type="protein sequence ID" value="MESCA005395-PA"/>
        <property type="gene ID" value="MESCA005395"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3415388_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005395-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="27" mass="3106" checksum="5F9BB958D2E8F283" modified="2013-10-16" version="1">MLVPEWRRSIGATCGRLLWQMVPSSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPA7</accession>
    <name>T1GPA7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPA7"/>
    <dbReference type="STRING" id="36166.T1GPA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005432-RA">
        <property type="protein sequence ID" value="MESCA005432-PA"/>
        <property type="gene ID" value="MESCA005432"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105581_0_0_1"/>
    <dbReference type="OMA" id="SWFLGQI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.170:FF:000001">
        <property type="entry name" value="Translation machinery-associated 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005432-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="20306" checksum="728C565B4F3BFC18" modified="2013-10-16" version="1">MGNLKKEFEKCKHPNSRRIKSVSKKAKRMNNKHKKQLGHAIKSNMMGEKLSWFLGQIEEDRAEPLTPQEFIDLIDEYLHRFDDELEQIKIKQSISKNRSTQHFARESAIKMTLDREVGEFNGGGMEFLNLCDPIKFKSLMDWDGNAINIQHLKFELISRGSLERLKAKGEMDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPH4</accession>
    <name>T1GPH4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005504-RA">
        <property type="protein sequence ID" value="MESCA005504-PA"/>
        <property type="gene ID" value="MESCA005504"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476787_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF11">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="6">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005504-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="21419" checksum="40601FDCAF6B9071" modified="2013-10-16" version="1">MRNQSWKEWSQIYQREESVPIIQCSGSPLIKMVGCSVQGCKSSQYNSRPENGGKKKHFFRFPKLRKDICDIWVERCRKEKINIGTAKICHLHFTKDDFILLYYNKVRLKEDAIPTLNLPVVPKLEPEEEIFLKISSLKWERRTCTRNDRTHQNSNGLRLCSIQELRDKESPSSLDQSSNMGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPS0</accession>
    <name>T1GPS0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02178103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPS0"/>
    <dbReference type="STRING" id="36166.T1GPS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005611-RA">
        <property type="protein sequence ID" value="MESCA005611-PA"/>
        <property type="gene ID" value="MESCA005611"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_2_1_1"/>
    <dbReference type="OMA" id="TWAIRKL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000084">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF140">
        <property type="entry name" value="FI02848P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577070" description="Peptidase M14 domain-containing protein" evidence="12">
        <location>
            <begin position="18"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="91"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005611-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="390" mass="44062" checksum="081C7401245EA3DB" modified="2013-10-16" version="1">MWLKLLSIVPLISASLALKGYQVIEIEPKFHDDFNEIENLQESYDFLKSSRTGKSQILVSPSESKLLQGTLENLNISYEIINKNILSDILQEKEDNDIFCVSARLGTDCYRSHSEINNYIEDLYQDIQTGHLSSKLGTPMNVVIFVDAGMHAREWISPATGIYVIQQLVENFEENQVFLKDFDWVVMPMINADGYEFTRSHPDNRMWRKNRQPYTEKCTGTDLNRNFDYQYGVSGTSSDPCVDTFKGSGPFSEPESVVLRDVLHSLKGRISFYLTLHSYGPQFLHPWSYAKVDAPTDAELSDVGRAGYEAVLDYSGRVYQYGNTAKLTYAAAGESSDYAYAVAGARISITMELPGGGKRGFDPPPKDIKEYVEESWAGIKAMAKRVIEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ84</accession>
    <name>T1GQ84_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ84"/>
    <dbReference type="STRING" id="36166.T1GQ84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005788-RA">
        <property type="protein sequence ID" value="MESCA005788-PA"/>
        <property type="gene ID" value="MESCA005788"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022670_0_0_1"/>
    <dbReference type="OMA" id="CAPVFPM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02396">
        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="74"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="279"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="358"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="447"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="362"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005788-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="511" mass="52688" checksum="6D12BA052805E01B" modified="2013-10-16" version="1">QEVGSIIGKKGEIVNRFREESGAKINISDGSCPERIVTVSGTTSAIYTAFSLITKKFEEWCSQFNDVGTSGGKTQIPIRLIVPASQCGSLIGKGGSKIKEIRNTTGCSIQVASEMLPNSTERAVTLSGTADQITQCIYQICLVMLESPPKGATIPYRPKPQVSGPVILANGQAYTIQGNYAVPAQETCTVFPLALAAGNHLHAGISGLTDPLVKGAHMGPPGAMPPHHIQHIPDVSKNPLANLAALGLAGIAPTTAGLNPTALAALAGSQLRTNNANRSQQQHEMTVPNDLIGCIIGKGGTKIAEIRQISGAMIRISNCEERDGGNTDRTITISGNADSVALAQYLINMSVELQKANLQEASSSTSSNGNQTSPTTSPVNNNNNNSSSPTSTSISNNSAIASAIPIAQLLSKPGALNNALGGLNLNTLGSLTDLITSLVTQCQAQQQTSSDNNTQDSNGSASSVANSATTNTTTNSTSTANAAAQLLTNNVLGQAFNNRQNSNGKSGTGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQK6</accession>
    <name>T1GQK6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vein beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQK6"/>
    <dbReference type="STRING" id="36166.T1GQK6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005915-RA">
        <property type="protein sequence ID" value="MESCA005915-PA"/>
        <property type="gene ID" value="MESCA005915"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_954998_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057777">
        <property type="entry name" value="Beta-barrel_vein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24700">
        <property type="entry name" value="Vein_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vein beta-barrel" evidence="2">
        <location>
            <begin position="224"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005915-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="292" mass="34284" checksum="4FEF9E20A0C87B54" modified="2013-10-16" version="1">MNARILRKWGLQKFVMSSQWIAVLTILVVIFVDKCATIDNNRRIEYPNMRPFRRKPPSLSLLPSFSESSVAAAAVPSVPKFHSQNIHNDNLKSFETSLTSSANIDVRNAFLNIPPTYIHNNIKNNNFKNLNSQLHLHPLITKLTPVNDINLNYKEPDDSSYRYRRNSMRSSQASSSYQHHQQQQQQQRRINNHQHQPHRRQLHFKSRPPKRRNRRRYCSARDPEQNGSGECDIYRESLKPRGLVRENELEIGRLYYLFVEQIDLANFTILGQPIRKTKHTKEKLERGINSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR39</accession>
    <name>T1GR39_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR39"/>
    <dbReference type="STRING" id="36166.T1GR39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006111-RA">
        <property type="protein sequence ID" value="MESCA006111-PA"/>
        <property type="gene ID" value="MESCA006111"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1410308_0_0_1"/>
    <dbReference type="OMA" id="AGPSHEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006111-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="21179" checksum="77B5808C9F6B92C8" modified="2013-10-16" version="1">MDPCIVSQASQIVSYFPRLLAFYCAISPPDLWPTDFGETGSIHGFPTYDFIIVGAGVGGSVLANRLSANPKWKVLVIEAGDNPPVDSQIPGAPPFEGENSTSTWNFYSQPSSKFGLGYKGRKLYWPSGKSIGGSGSINGMAYVRGNRLDYDIWESKGNKGWGWSSVLPYFEKSLKLHGDSRLILSRFPYKNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR45</accession>
    <name>T1GR45_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bardet-Biedl syndrome 7 protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR45"/>
    <dbReference type="STRING" id="36166.T1GR45"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006117-RA">
        <property type="protein sequence ID" value="MESCA006117-PA"/>
        <property type="gene ID" value="MESCA006117"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018704_1_0_1"/>
    <dbReference type="OMA" id="KGEGCFK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056335">
        <property type="entry name" value="BBS7_hairpin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056333">
        <property type="entry name" value="BBS7_pf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056332">
        <property type="entry name" value="Beta-prop_BBS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16074">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16074:SF4">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23349">
        <property type="entry name" value="BBS7_hp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23361">
        <property type="entry name" value="BBS7_pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23743">
        <property type="entry name" value="Beta-prop_BBS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BBS7 beta-propeller" evidence="3">
        <location>
            <begin position="21"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 platform" evidence="2">
        <location>
            <begin position="459"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 helical hairpin" evidence="1">
        <location>
            <begin position="559"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006117-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="77649" checksum="E6C7D4BA38C1D2D5" modified="2013-10-16" version="1">MELELTRIDYGNIGTSSANCLKLIPNEYKKQQKVVVGDLDGSLQFFSIKKSEAQIAYQSYVGKKVACIQLGGAPGTTMDKVFVATDNVVKGYNKKGKMFLSFDTNLTENIKCMYVSGTDLITCGNHVYSHYRDCKDVGTYLCGDTIVDVVALCQIIVLRVLEHCRVRQTIELESVPTVLHVPRSFPTDRILCGFADGKVSIFYFGNFTGDVKQEVLIKNELNSSAITCLDTYDLDGEGKDQLIIGRRDGTVQIYTMPMGNELDVEPRQIYKEVFPESISAIQAGCVYSNGFIEIVVLSYNGSIFGLTTQVISVSLSDKEVIKKIVKRPDDTNAIESQVIEGNDENQTISLVKPKENYKINSQLMNLGVSSTNLLDITSQLNLSQESGTMNLFLEIPYSIETVTLQSEVSLELAYFDKSIGEIIDSSTPTGYQFSAKITLKTETNRFEANFQTIEGQYGNQDRPYNTLSLKGSFSLAEIHNWLEQCIPEIPEKLGGANEPNKFYFKNVLIGSLLECKFSKGQANFKSDNLTTISILKDFLTKEATKKRIKLEISPEINNASIPHILKLVAPKIIDFLHIRKELKFLRALRQVDIKTEEDFNCLMPEYQSILNRAEEIEAAAAKHIPTELNRYFGILTDYYIDCFKFKGIFVKDRVNQLLEVLDEFELDKVIAFFNNSGQLDSSGSSGAANIEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRG1</accession>
    <name>T1GRG1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRG1"/>
    <dbReference type="STRING" id="36166.T1GRG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006245-RA">
        <property type="protein sequence ID" value="MESCA006245-PA"/>
        <property type="gene ID" value="MESCA006245"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076445_0_0_1"/>
    <dbReference type="OMA" id="QSPYEIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056953">
        <property type="entry name" value="CUT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF2">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25057">
        <property type="entry name" value="CUT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZP" evidence="1">
        <location>
            <begin position="61"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="316" mass="34860" checksum="1378649C6008AD81" modified="2013-10-16" version="1">MLTFNVDFSRCHSYDYGDTGDMVCRLSHHSRATLSDVQDPYLEVPEAATYELSSCYNVSIECGAVDMIARIRTSKMFNGKVYAKGSPKSCAVDVKNSLDFELRMNYNDLECNVRQQGAGRYINDVIIQHHSMIVTSSDLGLAVTCQYDLTNKTVSSDADLGVKGDIEPALSEEVIVDSPNVVMRITSRDGGDIMRTAEVGDPLALKFEIVDAQSPYEIMVRELVAMDGTDNSEITLIDSRGCPTDHFIMGPIYKSSLSGKVLLSHFDAFKFPTSEVVQFRALVTPCMPSCEPVQCDQEDISGELKSLISLDVKNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRP4</accession>
    <name>T1GRP4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">alkaline phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02391970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRP4"/>
    <dbReference type="STRING" id="36166.T1GRP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006332-RA">
        <property type="protein sequence ID" value="MESCA006332-PA"/>
        <property type="gene ID" value="MESCA006332"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_4_0_1"/>
    <dbReference type="OMA" id="EDAEFWH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF83">
        <property type="entry name" value="ALKALINE PHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006332-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="485" mass="53204" checksum="F19FF1E18EFFE994" modified="2013-10-16" version="1">MPIHDTEHLELRRSDDSVKMMQLTITHLAKCHHGREVPKNEDSIFWLQNGRDTLNNRLRMISKEDNLKAKNIILCLGGGMGLTTITAARIYKGQVAGQSGEEASLVFDNFPYSGLAKVYNTDTQVPDAGSGVTAIYTGTKTKQWALSMDNTCNLQNSKAGSLSTFMEWAQEAGKRTGFVTNTRISNPPLAGLFSKICNSRWECDSSIPQSFRGVYNDSSLQFLENGRKFNVVLAGGLSSMGAYDSEEQLSKILPDPNPPFCLFRNGHMSYSIDRQAEEPSLTEMTQKAIEILNVPDSKGFVLLIENGHIDHAHHMNLAEVALNEVLEFDRAIQLALKMTNESETLVIVTSDHSQPMTIIGDPKRGNDILGYTDESKTYETLTYSNGPELGLLPTGASQSTGNKIFRSHRSLYITNISTHSGEDVPVFAKGPGSALLGGVYEQSFIGHVISYAGSMGPNKEAAILSGVDVLKGVWYILAMCFVFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRQ8</accession>
    <name>T1GRQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-terminal amino-acid N(alpha)-acetyltransferase NatA</fullName>
            <ecNumber evidence="4">2.3.1.255</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-alanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50500"/>
            <dbReference type="Rhea" id="RHEA-COMP:12701"/>
            <dbReference type="Rhea" id="RHEA-COMP:12702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-cysteinyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-cysteinyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50512"/>
            <dbReference type="Rhea" id="RHEA-COMP:12707"/>
            <dbReference type="Rhea" id="RHEA-COMP:12708"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:65250"/>
            <dbReference type="ChEBI" id="CHEBI:133372"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-terminal L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50504"/>
            <dbReference type="Rhea" id="RHEA-COMP:12703"/>
            <dbReference type="Rhea" id="RHEA-COMP:12704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-threonyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-threonyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50516"/>
            <dbReference type="Rhea" id="RHEA-COMP:12709"/>
            <dbReference type="Rhea" id="RHEA-COMP:12710"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64739"/>
            <dbReference type="ChEBI" id="CHEBI:133375"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-terminal L-valyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-valyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50508"/>
            <dbReference type="Rhea" id="RHEA-COMP:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12706"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64741"/>
            <dbReference type="ChEBI" id="CHEBI:133371"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal glycyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50496"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133369"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.255" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02048390">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRQ8"/>
    <dbReference type="STRING" id="36166.T1GRQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006348-RA">
        <property type="protein sequence ID" value="MESCA006348-PA"/>
        <property type="gene ID" value="MESCA006348"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_7_2_1"/>
    <dbReference type="OMA" id="TISHRWR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="42"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="211" mass="24446" checksum="6C5ED9BA08238F30" modified="2013-10-16" version="1">MLVSHIKLKKCIECGLIEILELLRKKIRLRKSIQQIYKSIKMNIRCAKPEDLMNMQHCNLLCLPENYQMKYYFYHGLTWPQLSYVAEDDKGNIVGYVLAKMEEPEPGDESKHGHITSLAVKRSYRRLGLAQKLMNQAGQAMVESFDAQYVSLHILEIEPKYYADGEDAYSMRRDLSEMAKEICPDRDFSANKTETNRGHGHDHAHHDGHCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSA9</accession>
    <name>T1GSA9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HAP1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSA9"/>
    <dbReference type="STRING" id="36166.T1GSA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006561-RA">
        <property type="protein sequence ID" value="MESCA006561-PA"/>
        <property type="gene ID" value="MESCA006561"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185535_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF13">
        <property type="entry name" value="BICAUDAL D-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006561-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9813" checksum="77810EB584142781" modified="2013-10-16" version="1">MDKKKDFALDMEARSNQRTENADVWTQLQQKEADILLAAELGKALLEKNEELVKQQEKLIEEYNAKLEVGFRFLFQLIILMGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSC5</accession>
    <name>T1GSC5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSC5"/>
    <dbReference type="STRING" id="36166.T1GSC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006578-RA">
        <property type="protein sequence ID" value="MESCA006578-PA"/>
        <property type="gene ID" value="MESCA006578"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987159_0_0_1"/>
    <dbReference type="OMA" id="CIFDEYP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015375">
        <property type="term" value="F:glycine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF313">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006578-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="13832" checksum="D92053AB25CC3106" modified="2013-10-16" version="1">CLFCRDSLIVAVSNIATSFFAGLVIFSIIGFLAHELNVDVEKVVDQGAGLAFIVYPEVVTRLPISPVWSILFFVMLLTLGLDSQFALMETVTTAILDKFANLRNHKFWVVLIVAIFGYLGGLGFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSZ8</accession>
    <name>T1GSZ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006814-RA">
        <property type="protein sequence ID" value="MESCA006814-PA"/>
        <property type="gene ID" value="MESCA006814"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2298724_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006814-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11264" checksum="8BF2B06703AD67B6" modified="2013-10-16" version="1">MASRMCSQPATTTAEKLFLLTGNENNGGANMAVRNNTMSTWHQRMCHQNKDNLHYLPHITILTGTSADMANIVYVSCSSLYCNAEIRKMKQKLSSSFSITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTF2</accession>
    <name>T1GTF2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTF2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006982-RA">
        <property type="protein sequence ID" value="MESCA006982-PA"/>
        <property type="gene ID" value="MESCA006982"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1995208_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006982-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14342" checksum="C6341A77DFA467B6" modified="2013-10-16" version="1">MVMVPTESVTDVSSADLICRFLTLLLEALIGLLPMVINIWRNQQLFHLSVTPYDSSADHILHFNTLNYPRPSTSVLDYSTLFSLPSLVFGCNMQALFLHQLVLESTPLLFRYSRHQYQLQNIDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTH5</accession>
    <name>T1GTH5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02101667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTH5"/>
    <dbReference type="STRING" id="36166.T1GTH5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007007-RA">
        <property type="protein sequence ID" value="MESCA007007-PA"/>
        <property type="gene ID" value="MESCA007007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041844_5_1_1"/>
    <dbReference type="OMA" id="YPLYAFY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF24">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 3, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="15"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="286" mass="32840" checksum="76F73D38CBD54476" modified="2013-10-16" version="1">MDEEDLKKYDGQENVLLMMNHTYEIDWLAGWQFTEKMRVLGNCKAYAKKVISYVPVIGWTWYFSEFIFLNRSYDKDKEIIDKQLREVFSFPDPVWLLLNAEGTRFTEKKHEASVKFAQEKGMVPLKHHLIPRTKGFTASLPGLREKCDAIYDINLAFKKDADQVPEKEEEAAKFLQNLFVEKDKIIDSFHNTGSFFTESGFKEPASKLMVPRIYTLLNFIVGALVSILPLMYYLINSLLSANWIGFFIVVSILVGITLMMKKSIGMSKISKGSAYGSSVSHHQKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTP4</accession>
    <name>T1GTP4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02016040">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02016041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTP4"/>
    <dbReference type="STRING" id="36166.T1GTP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007082-RA">
        <property type="protein sequence ID" value="MESCA007082-PA"/>
        <property type="gene ID" value="MESCA007082"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212943_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF125">
        <property type="entry name" value="DOPAMINE D2-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12621" checksum="B29A59DE188E23E6" modified="2013-10-16" version="1">MLLYDGIITSLRVKDEPEDIFIRKKEDCGKDRVVRKTSCVFGMLVAILHLQHFRCDVYKVRTGLQSRNNMFILTTWLGYMNSFVNPVIYTIFNPEFRKAFKKIMHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW40</accession>
    <name>T1GW40_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02108550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02108551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008011-RA">
        <property type="protein sequence ID" value="MESCA008011-PA"/>
        <property type="gene ID" value="MESCA008011"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834062_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008011-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7108" checksum="66A9EEEB3F36410E" modified="2013-10-16" version="1">MKSTSPLHPVNICSSSHNEHISICRGDSGGPFVKEFPNGSPVQGARESKTLRLYYVEKSMGCNAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXR3</accession>
    <name>T1GXR3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008618-RA">
        <property type="protein sequence ID" value="MESCA008618-PA"/>
        <property type="gene ID" value="MESCA008618"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888133_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008618-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15904" checksum="A48ED7CB94CB0D51" modified="2013-10-16" version="1">MILTSFDREGEDVENESSFENIFFNYFIRKMVDEKDYYHSTILFINIGILLYELAKKNLILEYTPPFKGSGFDDWQFRVKMYMKSSNLLAALTEDEPAEVNAKTEFNKMNDKAMNRLVSFVHNDCLCHTREKETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY38</accession>
    <name>T1GY38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diacylglycerol kinase</fullName>
            <shortName evidence="7">DAG kinase</shortName>
            <ecNumber evidence="7">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02155315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GY38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008758-RA">
        <property type="protein sequence ID" value="MESCA008758-PA"/>
        <property type="gene ID" value="MESCA008758"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003770_3_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF118">
        <property type="entry name" value="DIACYLGLYCEROL KINASE EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="8">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008758-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="30087" checksum="A71EA65B38A3B542" modified="2013-10-16" version="1">MEISNKGPSEALKLTIKSCPTNSKILVAGGDGTVGWVLNTIKQMNIVPHPSIAIMPLGTGNDLSRVLGWGPEPPSTLDPLKILRSIQKAEPIKLDCFDMIVKQHKRLSLPKKSFTSKYQIYNYFSIGVDALVTYNFHKTRESPFYLLSSRLFNKMIYFWTVVKSNIAFLMGKWKXXXXXXXXXXXXXXXXEHSISDGKMEVFGVASSFHVAQLQVKLSKPIRLGQGRNIKITLKKCVAVQADGEPWLQKPCDIFISSTGQVEMLQNRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY57</accession>
    <name>T1GY57_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008779-RA">
        <property type="protein sequence ID" value="MESCA008779-PA"/>
        <property type="gene ID" value="MESCA008779"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906642_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008779-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6717" checksum="E2CE670CA03E10E5" modified="2013-10-16" version="1">MSSLSASVENLSGSDHQSSQKSNDSSSMCLELALEARNIFLKGTPINFINKTRNIMFGIPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYS3</accession>
    <name>T1GYS3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009010-RA">
        <property type="protein sequence ID" value="MESCA009010-PA"/>
        <property type="gene ID" value="MESCA009010"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852280_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7830" checksum="5AA1E3A2AF167D66" modified="2013-10-16" version="1">MNDVYFTELEIVINKEFGFVAAYLPDSGPRPFITCYEPEVYDILARVQYIGLTGRDANAVRYIYDCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ60</accession>
    <name>T1GZ60_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009156-RA">
        <property type="protein sequence ID" value="MESCA009156-PA"/>
        <property type="gene ID" value="MESCA009156"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3282412_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009156-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="4036" checksum="9C1EB3561D297684" modified="2013-10-16" version="1">ATGPLLKTPLSQAQQAYTAATTYTAVRTAFGAAAAAAQPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ62</accession>
    <name>T1GZ62_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zasp-like motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ62"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009159-RA">
        <property type="protein sequence ID" value="MESCA009159-PA"/>
        <property type="gene ID" value="MESCA009159"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1911906_0_0_1"/>
    <dbReference type="OMA" id="RVFQKIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009159-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15167" checksum="DCF5EC3B04709EF4" modified="2013-10-16" version="1">TPSGNTTPNQSKSFRILQKITSTEDGSDDFINQMNQPAELQKPHYSRQMMATPQQQTNNAAIEQLQRMQQNRESGRQGPPMKYPQPPTYVQQRAPYQNDNEQYIPPSQQQAPEPKIYTGSAIPSRSFKLLQAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZI4</accession>
    <name>T1GZI4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009285-RA">
        <property type="protein sequence ID" value="MESCA009285-PA"/>
        <property type="gene ID" value="MESCA009285"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062715_0_0_1"/>
    <dbReference type="OMA" id="VEPDCKP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF29">
        <property type="entry name" value="IP17226P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577258" evidence="2">
        <location>
            <begin position="19"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="253" mass="28897" checksum="96F23F4C3250BF3D" modified="2013-10-16" version="1">MKYLFVLSLLLVFQNSSALNAKEIEQFLEDLRLRMCHPIEKLGLPALDPMTKKNLEEFNVNIKDFLMIDGWTRRFELLGLSDFIIQDIKFPGLLKKSLVIDLLFPNTTFFSDYKANGTAGYVFPIDGRGDLQGYVTDFHFRVEGKVKVGLKGISMSDFKLIFGVKEIYLSAERFVPDERINDFLHAVVNELGLELVQYIWNETHETLTKLSKKINQYLKDKPLSEIIGGGSGEPIFGEDAPPPDCKPDWNPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZL1</accession>
    <name>T1GZL1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Salivary secreted peptide</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02372840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009314-RA">
        <property type="protein sequence ID" value="MESCA009314-PA"/>
        <property type="gene ID" value="MESCA009314"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152785_3_1_1"/>
    <dbReference type="OMA" id="NVTAQIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031734">
        <property type="entry name" value="MBF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37685">
        <property type="entry name" value="GEO11136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37685:SF1">
        <property type="entry name" value="GEO11136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15868">
        <property type="entry name" value="MBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577853" description="Salivary secreted peptide" evidence="1">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009314-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13459" checksum="8AC6CDB80ADB03B2" modified="2013-10-16" version="1">MTFLYKLTIFSLFFASVLAFDRSWGHRQNEDYLVDSEEVFAKANGENGRVSVEIVFPRYGKELSETITYIEVVDNFRNSSNGDVILFQGGPNFHYAAFLFDSQKGYGIDYTLKIWAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZP0</accession>
    <name>T1GZP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TRPM-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZP0"/>
    <dbReference type="STRING" id="36166.T1GZP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009344-RA">
        <property type="protein sequence ID" value="MESCA009344-PA"/>
        <property type="gene ID" value="MESCA009344"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763441_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF1">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL TRPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="TRPM-like" evidence="6">
        <location>
            <begin position="2"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009344-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="16950" checksum="806A0BDD0B1D3000" modified="2013-10-16" version="1">FCYRTDAEKAQRLLTCELHSWSNQSCLSLAVAANHRALLAHPCSQVILADLWMGGLRTRKNTNVKVILGLLIPIFIKKLDFKSREELQQMPQTEEEHLENQNLDYEDSERSHPDAETPINKAKRSISLRYRKGSNASQVVDKFINCIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZR4</accession>
    <name>T1GZR4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009369-RA">
        <property type="protein sequence ID" value="MESCA009369-PA"/>
        <property type="gene ID" value="MESCA009369"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006510_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009369-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14700" checksum="EB3F83144FF5D589" modified="2013-10-16" version="1">MSPHKALFGHDKICHGSSYELLKKLDCLNEPDFKVYSDPDKIKKIQNSIMDKIRMAHERNEKRYNLRVRKPLWKIGQVVFRRLFPQSSMIKNFNANVTSPLPCEYKTLLRVTNINKYFSIKMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H034</accession>
    <name>T1H034_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H034"/>
    <dbReference type="STRING" id="36166.T1H034"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009500-RA">
        <property type="protein sequence ID" value="MESCA009500-PA"/>
        <property type="gene ID" value="MESCA009500"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011689_0_0_1"/>
    <dbReference type="OMA" id="FARWDTE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09566">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09569">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000007">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037618">
        <property type="entry name" value="LIPB1/2_SAM_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037619">
        <property type="entry name" value="LIPB1/2_SAM_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF14">
        <property type="entry name" value="AT31531P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="61"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="144"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009500-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="40736" checksum="699C307AFD15CA47" modified="2013-10-16" version="1">LYFTGIKHIWGKLRRCNSGNLEEYLLDDSFRRGSNIRSTTNGRIEYPTKLNEASLKSYRLWNLQDICNWLSHLQMDIYINDCRRWLGKGNHQDIFDVSPIDISRELNLKHQLHRKKIILALNELVGKETDITATNAGKLNNIWVIRWLDDIGLPQYKDTFMEAIIDGRMLHKLTMDDLNQLQVTSCLHIASIKNAIFVSGRLNNWKPDSLIRRSKEDNSSNENSKVYLWTAHRVMEWLKAVDLAEYAPNLRGAGVHGGLIYFEPRFNADLLADLLSIPSSKTLLRRHLATHFKELLGHEKIKEKRDAESNPNIQSLTIAAKMKFPKKTQFSLKRRKSSKSLKILIGLTMFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Q3</accession>
    <name>T1H0Q3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BLOC-1-related complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORCS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Q3"/>
    <dbReference type="STRING" id="36166.T1H0Q3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009738-RA">
        <property type="protein sequence ID" value="MESCA009738-PA"/>
        <property type="gene ID" value="MESCA009738"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150749_0_0_1"/>
    <dbReference type="OMA" id="HLNYQQE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032143">
        <property type="entry name" value="BORCS7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31397:SF1">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31397">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 7 BORSC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16088">
        <property type="entry name" value="BORCS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009738-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11307" checksum="753CCCD0F201224B" modified="2013-10-16" version="1">MTSASSSSAKHLFADSKNRLAERVNQNVSNIGSVARQIVRGSKSNEILGQAAKHMAQCETLMDNTATTLHKIELIRQHIGYQHEAIQDSIEQLDYLQEQVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H116</accession>
    <name>T1H116_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02372968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H116"/>
    <dbReference type="STRING" id="36166.T1H116"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009867-RA">
        <property type="protein sequence ID" value="MESCA009867-PA"/>
        <property type="gene ID" value="MESCA009867"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_608626_0_0_1"/>
    <dbReference type="OMA" id="WLAIFLC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF31">
        <property type="entry name" value="IONOTROPIC RECEPTOR 68B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009867-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="45917" checksum="E9959BFCD2F66B67" modified="2013-10-16" version="1">MTKNFYSWGNLKRVIVDENITLICDRNLDNFPKHFNGFPQRVFMFQEDPVAVNWDRFPKSFQNSYLGKHRKQNPMTAGYNGLTMAGLVEYINFTPLYIVPKLEADKYAYKDNEGLIKGSGEVSYSKFDIVGMLHFITDNQGMDYEFLHPIFFDNYCVVAPIANSMPLFIASMKCFTLGSWIFIFAIPYISAFILKILNKFNPSHDGLKVSNFVQLTGLLTFVLISITANIKTKSSMQRWLLSGYKRYNEINDLRSLSESSFDIVINSPFMYNIAFGDESRAVDPIMKKLQSKLRLGVDSVYQTVERKACVFRMKSDYEIVNMKIQSQFGEEKLHLVNECPRSYHSTFLVRKGWSFSPLFNRRIINLVESGLIDFWKESSKFALNLNSTIELHEHGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H166</accession>
    <name>T1H166_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H166"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009920-RA">
        <property type="protein sequence ID" value="MESCA009920-PA"/>
        <property type="gene ID" value="MESCA009920"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856322_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009920-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7465" checksum="0B87A48CBCBD44B9" modified="2013-10-16" version="1">MTSENDAIAYRSEWLELINSAAHSNLDNLKKLAFSGSLKNTKFRTISWSILLRVLSNDPSEWRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1A1</accession>
    <name>T1H1A1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">KELK-motif containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1A1"/>
    <dbReference type="STRING" id="36166.T1H1A1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009957-RA">
        <property type="protein sequence ID" value="MESCA009957-PA"/>
        <property type="gene ID" value="MESCA009957"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_693673_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031597">
        <property type="entry name" value="KELK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF66">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GENGHIS KHAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15796">
        <property type="entry name" value="KELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KELK-motif containing" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="158"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="360"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009957-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="398" mass="47273" checksum="897D79D9FF8DE03C" modified="2013-10-16" version="1">QQEKEDLEKEKVDASDRLKQQELELNDALCQRNLAMKEYSEVTDKLSELRSQKQKLSRQVRDKEEELETTMQKVDNIRNDLRKSDKMKRDLEAKVEDLMADLSKERKVRERAEEYCRQLQTEMRSINHPSDFGSSSSLGISSAESTRLELERIDVQYNEKLNQQQTRFNMELSSLREQLNEAEKHRDLLYRELQSVREKFDSNRLENLNDSEETLGELKRNFEQEKSLWSEERQKLYEEMEEHKENIRRLQIQQQNDMEEIRHKNHCIIQWEHQMGEILQWVSDEKDARAYLQALTSKMSDEVDYLKHSSEFIPGLLHKNSSASDNKNWKNRRSQKLDKMELLNLQSSLQSEIQAKAAISEELSRTRADLVAALKNLEETQQKCDVIAQEMKTKDMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1L8</accession>
    <name>T1H1L8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1L8"/>
    <dbReference type="STRING" id="36166.T1H1L8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010081-RA">
        <property type="protein sequence ID" value="MESCA010081-PA"/>
        <property type="gene ID" value="MESCA010081"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161223_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039857">
        <property type="entry name" value="Ift122/121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12764:SF5">
        <property type="entry name" value="LD29485P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12764">
        <property type="entry name" value="WD REPEAT DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010081-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="13228" checksum="82357892CA12A3D8" modified="2013-10-16" version="1">MRRERNDNNVATLHPVTHDLAVDLRSTLCDWFRVVQLYRLGTGTSDQQLEKAWQEIGKHFANLNSWESAKEYFQKSHDMEGLIDALYHLEQYDELYECVGRVPEKLHFFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1M8</accession>
    <name>T1H1M8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02376917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1M8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010094-RA">
        <property type="protein sequence ID" value="MESCA010094-PA"/>
        <property type="gene ID" value="MESCA010094"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256775_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="50"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010094-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11215" checksum="2E3F5232E1C5033E" modified="2013-10-16" version="1">MVRGHLFFLVFLKDLSEKCVGGRFFGNKTPFIGSYTYDCDIRKLVNPTLQICAGGNGSDTCQGDSGGPLMFLDRTSFVKPYNILIGITSFGSICGGPVSGVYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1N4</accession>
    <name>T1H1N4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1N4"/>
    <dbReference type="STRING" id="36166.T1H1N4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010100-RA">
        <property type="protein sequence ID" value="MESCA010100-PA"/>
        <property type="gene ID" value="MESCA010100"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_0_3_1"/>
    <dbReference type="OMA" id="ISADEKC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="34"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010100-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="30737" checksum="BDCBEB07B9E55191" modified="2013-10-16" version="1">MGLIYYIVPPATGVRAKAACTAYKNRGLYLRNFIYNSESAEYGIFPHMAALGYSHNSEPYKWNFGGSLISRQFVLTVAHCIDDSLKVVRLGLADLTEENHSWNDYYIKAPYIFPNYQKRLPYNDIALIRLNKNVEFHNFVHPACLNTDFEKKFNLSLTLTDPDYKSKKIPILQKVNLTVINFEKCKEIIGRDNRLILGLHQSKLCTNDFGRRDTCRGDSGGPLQTLQANISVSTIIGIISSGVYCATNVPSLYTNVSYYLDWIENIVWPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H200</accession>
    <name>T1H200_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H200"/>
    <dbReference type="STRING" id="36166.T1H200"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010228-RA">
        <property type="protein sequence ID" value="MESCA010228-PA"/>
        <property type="gene ID" value="MESCA010228"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001442_6_0_1"/>
    <dbReference type="OMA" id="DNSWNLN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="289" mass="34020" checksum="E4709EE02F10FA5B" modified="2013-10-16" version="1">MCKIVPPSEWLPRRIPYNINDMDFSIKTPTEQVFKQKSNGVYKLKNQIIDKLSLVQFKDMTYLKKYKPPKFSSFEEIESLYWEEISNKNAVHPIYAADIQMTLMDFNLKYWNLNSLNSILDLVDKEYWIDIDGLWYVIPPSYGKQFESLLKKLYPKEFEECSSFMRHKTIMVHHSILKKHGIPYNTVLQEAGDIIITFPFAYHCGFNLGFNAAESTNFATERWIEYGKRTKFCKCFVDTVKISMQIFVQRFQPDKYEAWLSGKDYGSHPEEPDVICPANKATINIYVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2G7</accession>
    <name>T1H2G7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2G7"/>
    <dbReference type="STRING" id="36166.T1H2G7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010412-RA">
        <property type="protein sequence ID" value="MESCA010412-PA"/>
        <property type="gene ID" value="MESCA010412"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1157569_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF24">
        <property type="entry name" value="IONOTROPIC RECEPTOR 60A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010412-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="28405" checksum="4EFFF205882283F5" modified="2013-10-16" version="1">MVNNRICDITFGGYYPDNEVHEDFWTTDNYLEDPFRFYIKLAGLEEPWRGLATIFEWDIWVLSFFTLVICWILWFLFGMISKRESSDHRSQSLTALNTFALSINVSVFERPEHNPLRLLFMALTLFALNLTCIYSSKLIDVFTRPAYVHQIDTIEEVMELGIPFGGPKESRDWFENDDDIEIFKLITILNYLIFDHQIWQQLQEAIACYWSTECICFKMQTTVKSMLFPILLLLFLQKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2H5</accession>
    <name>T1H2H5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02372793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2H5"/>
    <dbReference type="STRING" id="36166.T1H2H5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010421-RA">
        <property type="protein sequence ID" value="MESCA010421-PA"/>
        <property type="gene ID" value="MESCA010421"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388700_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010421-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="11050" checksum="C52A1C60313D98C2" modified="2013-10-16" version="1">MISNAHFHNWWQRHVKILFNQPAPKIPRRNHRSIKVKQIFWFDHCIPVSPLGSGLTTGFACSIGIDMDNRRRNKSVESRQIDIVPYQREEDPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2S8</accession>
    <name>T1H2S8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TBC1 domain family member 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. The TSC-TBC complex acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02376303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2S8"/>
    <dbReference type="STRING" id="36166.T1H2S8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010532-RA">
        <property type="protein sequence ID" value="MESCA010532-PA"/>
        <property type="gene ID" value="MESCA010532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143732_0_0_1"/>
    <dbReference type="OMA" id="MIYWIAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039842">
        <property type="entry name" value="TBC1D7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13530">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13530:SF3">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="16332" checksum="12663B371249A6B8" modified="2013-10-16" version="1">MFDDDVMIYWIAKEFYVFSLEIKTDFYKHLDLFKNLLEKEDPPLFSHFINLNLLEDLPLEIWCTNGFAGVLSSPALVRFWDKICGGSRKIVAFVLLMLFKSIKKHVMEMSLASEIKDFVEKGNHQNDLIVNKAIEMWHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H322</accession>
    <name>T1H322_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H322"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010633-RA">
        <property type="protein sequence ID" value="MESCA010633-PA"/>
        <property type="gene ID" value="MESCA010633"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906641_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6601" checksum="2DB7142CA20C1D8E" modified="2013-10-16" version="1">MYFAYGTYNSVTSLTPCECKTLIRVTNIHQISSSFAPSKGGEHGSLCSGRHLAGHGTILAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H374</accession>
    <name>T1H374_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H374"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010693-RA">
        <property type="protein sequence ID" value="MESCA010693-PA"/>
        <property type="gene ID" value="MESCA010693"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3280072_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010693-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="4590" checksum="1981B565B40DA83F" modified="2013-10-16" version="1">MKLDAQAQGVIVTSLGDEAILHILNYETPGVMWLKLESIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H439</accession>
    <name>T1H439_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enhancer of rudimentary homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as an enhancer of the rudimentary gene. Has a role in pyrimidine biosynthesis and the cell cycle.</text>
    </comment>
    <comment type="function">
        <text evidence="5">May have a role in the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the E(R) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387213">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H439"/>
    <dbReference type="STRING" id="36166.T1H439"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011040-RA">
        <property type="protein sequence ID" value="MESCA011040-PA"/>
        <property type="gene ID" value="MESCA011040"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125703_1_0_1"/>
    <dbReference type="OMA" id="ESRTWSD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2260.10:FF:000001">
        <property type="entry name" value="Enhancer of rudimentary homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2260.10">
        <property type="entry name" value="Enhancer of rudimentary"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035912">
        <property type="entry name" value="EHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000781">
        <property type="entry name" value="ERH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12373">
        <property type="entry name" value="ENHANCER OF RUDIMENTARY ERH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12373:SF0">
        <property type="entry name" value="ENHANCER OF RUDIMENTARY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01133">
        <property type="entry name" value="ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016393">
        <property type="entry name" value="Enh_rudimentary"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143875">
        <property type="entry name" value="ERH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01290">
        <property type="entry name" value="ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011040-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12465" checksum="FF377230EEB259C2" modified="2013-10-16" version="1">MSHTILFIQPSQKPESRTYSDYESVNECMEGVCKIYEEHLKRRNPNTPTITYDISQLFDFIDQLADISCLVYQRSTNTYAPYNKEWIKEKIYVLLKQAAGTKINMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4A2</accession>
    <name>T1H4A2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4A2"/>
    <dbReference type="STRING" id="36166.T1H4A2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011110-RA">
        <property type="protein sequence ID" value="MESCA011110-PA"/>
        <property type="gene ID" value="MESCA011110"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387337_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011110-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="21907" checksum="7E69BAFA7509BD1F" modified="2013-10-16" version="1">PPYRPPSPRCRDCRQSLNASRTSSQTSLPSLNGIMQIAGMEQYASLRHPDGASDPNLPKTERNNDDEFNEKSSLLSKIPSAEQDDKSLLTDLPIISQLPLLPLNTNEAYDSGHDSSTPRTSKHSGISRRAESGYHSVATARDSDESSFTSASISKDHHNRITMKKSKQRHDKPSLCNWLMNPFTCTYPETEGEISDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4D0</accession>
    <name>T1H4D0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4D0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011139-RA">
        <property type="protein sequence ID" value="MESCA011139-PA"/>
        <property type="gene ID" value="MESCA011139"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199973_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011139-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12244" checksum="888FDEFD6C172A57" modified="2013-10-16" version="1">MDQTTYQFSGKFYRQCFGTSIGNPLSCFTANNFMANLAAQDVEAVFEVYKDRMVSSNPIIPRIYALYKTNKPGLRPIILKINSPSSRIAKFSSLKPPQGFYIKNSFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4J0</accession>
    <name>T1H4J0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein quiver</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382590">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4J0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011199-RA">
        <property type="protein sequence ID" value="MESCA011199-PA"/>
        <property type="gene ID" value="MESCA011199"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560872_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577985" description="Protein quiver" evidence="1">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011199-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9225" checksum="218A12072B7E9CF7" modified="2013-10-16" version="1">MYNVAISSLNLVVTSALHCWQCSSDTLGAEDFCGEVFKKDSIPEDVQRRNYLDVTKHCNSSGIHSEFLIPVCRKTVEENICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4V1</accession>
    <name>T1H4V1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein 198</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM198 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4V1"/>
    <dbReference type="STRING" id="36166.T1H4V1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011319-RA">
        <property type="protein sequence ID" value="MESCA011319-PA"/>
        <property type="gene ID" value="MESCA011319"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043600_2_0_1"/>
    <dbReference type="OMA" id="NHTMIDL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025256">
        <property type="entry name" value="TM7S3/TM198-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040236">
        <property type="entry name" value="TMEM198"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31247:SF5">
        <property type="entry name" value="DUF4203 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31247">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 198 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13886">
        <property type="entry name" value="TM7S3_TM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="TM7S3/TM198-like" evidence="9">
        <location>
            <begin position="36"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011319-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="38654" checksum="3E5DDA7A104967AD" modified="2013-10-16" version="1">MKNKLFSYSSLDLKGIMENPNCAPKSPDPLSALFWTTIAVFGIVYALLGYRCLRAIGFLSGLLVGANIAFVLQDFKVTLMGSPADSAFAILSGLIGAVLGSAHPIASALVSAFAGALLAATTMVVCVATMPDHTFGSKEIYVAIIGGAIIFSVLTLCCVKFITILASSIVGTAMILAALDFFLNDLETLKWLVTMAPNPQPPPCYGGILIFSWPVSVIVSLMVQCFITGWRVDHRKYVPRYRHHPPPRLPPLPPNSSRGNSMRNDVQMNTRQRKYRYLYQVRTARGDIISQNFVSALQKRVQQHGYGTPPSTASIKTSSHDRNTMRSDRTHFTNIPDTEMYDNKFDLVNERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4Z2</accession>
    <name>T1H4Z2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4Z2"/>
    <dbReference type="STRING" id="36166.T1H4Z2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011365-RA">
        <property type="protein sequence ID" value="MESCA011365-PA"/>
        <property type="gene ID" value="MESCA011365"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1528926_0_0_1"/>
    <dbReference type="OMA" id="NERICHR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF91">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011365-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="19863" checksum="2CD99BAAEB2C02E4" modified="2013-10-16" version="1">MQLKIADFGLATQLKRADEKHMTMCGTPNYISPEVVSRTSHGLPADVWGLGCMFYTLLVGRPPFDTDAVQSTLTKVVMSDFYLPEHLSFEAKDLIVKFLKKNPMERIPLENVMSHPFMVKNCNQYGEYNSTVSSMDSGIITFSSNNSSQPHQYRAPMKPLLSSTQHNQMQYDSIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H505</accession>
    <name>T1H505_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02384000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H505"/>
    <dbReference type="STRING" id="36166.T1H505"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011378-RA">
        <property type="protein sequence ID" value="MESCA011378-PA"/>
        <property type="gene ID" value="MESCA011378"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984097_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF59">
        <property type="entry name" value="INTEGRIN BETA-NU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0401">Integrin</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="9">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011378-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14275" checksum="36BF973AF519533D" modified="2013-10-16" version="1">MHFAGDGKLAGITQRNDKTCHLDESGEYLGSLLYDYPSLEEMYRELIKNKVSVIFAVTKSVLGTYQRIHELMPEISNVEMLTLDSSNILELLKSSYEGFIVGVCTIDSKKKMFAQMLKLGRNLTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5D5</accession>
    <name>T1H5D5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02134428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H5D5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011512-RA">
        <property type="protein sequence ID" value="MESCA011512-PA"/>
        <property type="gene ID" value="MESCA011512"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005078_1_0_1"/>
    <dbReference type="OMA" id="YCHDDAR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22091">
        <property type="entry name" value="F-box_FBXO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000003">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000011">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000087">
        <property type="entry name" value="GG17321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047505">
        <property type="entry name" value="F-box_FBXO11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF20">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00722">
        <property type="entry name" value="CASH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="916" mass="100262" checksum="B2D4679B0E9A4AF7" modified="2013-10-16" version="1">AAPSTNGPNTSSATAHQSSSSSATSSSASSSSSTSSSSKSSAPIPQNIDGGGSAASEGSSSSCHHQSPYDLRRKIPQDLWNGSSSATSSASTSNAGNPSSSSSCVPVNQTNNQAAFSPSFLEESSSSSSSTVNTSAQPLRKRPRTATVTSSSATTPSATGTSCSSQQVFESSLPPAAHYLQYEMPDEVLLTIFSYLFEQDLCRLSLVCKRFNIIANDTELWKRLYQSVFEYDLPLFNPEICKFIFLKPEDSEYSNPWKESFRQLYRGIHVRSGYQNKPYKSRKIIYFDTIATALEYPEDLKQKQTQSVLRFDQFDEDKNSSNTSNTNNQPIIFLHSGHYKDDYLFIDSDVAWIGAAPGSNVADNVILERESRANHAYLGFXSDFSPDNTSTVQHHKHYCLEIGENCSPTIDNCIIRSSSVVGAAVCVTGSGANPTIRNCDISECENVGLYVTNYAQGTYEHNEICKNALAGIWVKDYASPIMRENHIHHGRDVGIFTFENGMGYFEKNDIHNNRIAGFEVKAGANPTVVKCEIHHGQTGGIYVHENGSGQFIENKIHSNNFAGVWITSNSNPTIRKNEIYNGHQGGVYIFGEGRGLIEHNNIYGNALAGIQIRTNSDPIVRHNKIHHGQHGGIYVHEKGQGLIEENEVYSNTLAGVWITTGSTPVLRRNRIHSGKQVGVYFYDNGHGKLEDNDIFNHLYSGVQIRTGSNPIIRGNKIWGGQNGGVLVYNGGLGVLEQNEIFDNAMAGVWIKTDSNPTLKRNKIYDGRDGGICIFNGGKGILEENDIFRNTQAGVLISTQSHPVLRRNRIFDGLAAGVEITNNATATLEYNQIFSNKFGGLCLASGVHPITRGNKIFNNQDEVEKAVSGGQCLYKISSYTSFPMHVFIGVKRAILPIETLSVLTVSRIVMLAMMLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5V4</accession>
    <name>T1H5V4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bladder cancer-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a tumor suppressor; induces growth arrest at G(1)/S checkpoint and apoptosis via RB1-dependent and p53/TP53- and NF-kappa-B-independent mechanisms. Modulates expression of genes involved in the regulation of proliferation, cell cycle and apoptosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BLCAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5V4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012087-RA">
        <property type="protein sequence ID" value="MESCA012087-PA"/>
        <property type="gene ID" value="MESCA012087"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181908_0_0_1"/>
    <dbReference type="OMA" id="PCHICSI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009598">
        <property type="entry name" value="BCALP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13259">
        <property type="entry name" value="BLADDER CANCER 10 KD PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13259:SF1">
        <property type="entry name" value="BLADDER CANCER-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06726">
        <property type="entry name" value="BC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01396">
        <property type="entry name" value="BC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012087-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7147" checksum="07379B2D1AE56A40" modified="2013-10-16" version="1">MYCLQCLIPVLLIPKPSNPALLQTHVMFITLYLIGFFLERKPCTICSIVFLTAVFLICNSGIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H629</accession>
    <name>T1H629_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">40S ribosomal protein S4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H629"/>
    <dbReference type="STRING" id="36166.T1H629"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012162-RA">
        <property type="protein sequence ID" value="MESCA012162-PA"/>
        <property type="gene ID" value="MESCA012162"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_1_0_1"/>
    <dbReference type="OMA" id="GHIQLNL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="11"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 central region" evidence="8">
        <location>
            <begin position="57"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 C-terminal" evidence="10">
        <location>
            <begin position="152"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012162-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="22832" checksum="83FBBCDF7FCA6A64" modified="2013-10-16" version="1">TGPHKLRESLPLMIFLRNRLKYASAQVTKIVMQRLIKVDGKVRTDPTFPAYMDVVTIEHFRLVYDVKGRFTIHRIPEEAKYKLCKVRKIQLCHKGVPSAITHGRTILYPEPFIKANDTVWDLTTGKITDYRVGTVVNRERHPGSFDIVHIKDTQGHIFATRLCNVFIIGKGNKPYISLPKGKGVKLSIAEERDKRLAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6K3</accession>
    <name>T1H6K3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6K3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012336-RA">
        <property type="protein sequence ID" value="MESCA012336-PA"/>
        <property type="gene ID" value="MESCA012336"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091077_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012336-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="21638" checksum="639DFF0867547C9B" modified="2013-10-16" version="1">MSMYPKPPGQNDEAKYEVLRNPNLDGLARHFIGNLHRYRENIIIPKSMGPVQIKTNEEKLVESVFESCTFKSVVACVIGYGLGAAIGLFSASVNPNVADPFFAEKRQTAREIFRDMRASTHSYGKNFAIIGAVFSAVECCIETXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Q1</accession>
    <name>T1H6Q1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6Q1"/>
    <dbReference type="STRING" id="36166.T1H6Q1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012384-RA">
        <property type="protein sequence ID" value="MESCA012384-PA"/>
        <property type="gene ID" value="MESCA012384"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661610_0_0_1"/>
    <dbReference type="OMA" id="VFEIFIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012384-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="9099" checksum="FF0E6CAED6C75CF8" modified="2013-10-16" version="1">MPKRRDTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHKQGDVYEKSLKYINMGFTGMFSVETVLKIIGFGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Q7</accession>
    <name>T1H6Q7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Synaptic plasticity regulator PANTS</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Plasticity-associated neural transcript short</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Synaptic cleft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0545 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6Q7"/>
    <dbReference type="STRING" id="36166.T1H6Q7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012390-RA">
        <property type="protein sequence ID" value="MESCA012390-PA"/>
        <property type="gene ID" value="MESCA012390"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130047_0_1_1"/>
    <dbReference type="OMA" id="CHLYKDE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043083">
        <property type="term" value="C:synaptic cleft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012390-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12883" checksum="4647E79402A717BA" modified="2013-10-16" version="1">IRPCLIYKDEYSECTSIKGRFHQYFIHGENQDCSQWKKDFDNCIAYNESDQRNNQAAEEIIRSEENRRQIRLKAHFANDTWSKRKSPPEDWAAPLPEWIVKKNENTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H769</accession>
    <name>T1H769_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-fold modifier 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H769"/>
    <dbReference type="STRING" id="36166.T1H769"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012607-RA">
        <property type="protein sequence ID" value="MESCA012607-PA"/>
        <property type="gene ID" value="MESCA012607"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175114_0_0_1"/>
    <dbReference type="OMA" id="MEHAVGK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01766">
        <property type="entry name" value="Ubl_UFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000044">
        <property type="entry name" value="Ubiquitin-fold modifier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005375">
        <property type="entry name" value="UFM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825:SF0">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03671">
        <property type="entry name" value="Ufm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038027">
        <property type="entry name" value="Ubiquitin-like_Ufm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012607-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9038" checksum="3B1E2A63EC00FC0A" modified="2013-10-16" version="1">KVTFKITLTSDPKLPFKVLSVPEGTPFTAVLKFAAEEFSVPAATSAIINDGIGINPQQTAGNVFLKHGSELRLIPRDRVGHSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7A1</accession>
    <name>T1H7A1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sec1 family domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7A1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012639-RA">
        <property type="protein sequence ID" value="MESCA012639-PA"/>
        <property type="gene ID" value="MESCA012639"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_677203_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000002">
        <property type="entry name" value="sec1 family domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012639-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="46070" checksum="F7037EEF6BE41CB7" modified="2013-10-16" version="1">MLTLRQRQINAIKQMLNLNQQQPKGLAAEPVWKILIYDRVGQDIISPLISIRELRDMGVTLHVQLHSDRDAIPDVPAIYFCAPTDENLDRIQQDFQRGLYDIYHLNFISPIPRQKIEDLASAALASGCVANIHKVYDQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFCFXILCYLQNLPITKLTEQVMECRSGVEKDDYLYLDPKLLKGGDVMPKNRAPFQDAVVFVVGGGNYIEYQNLVDFIKQKQNANTTKRIIYGATTLNNSKQFLKELSELGREIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Z5</accession>
    <name>T1G9Z5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin I-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Z5"/>
    <dbReference type="STRING" id="36166.T1G9Z5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000037-RA">
        <property type="protein sequence ID" value="MESCA000037-PA"/>
        <property type="gene ID" value="MESCA000037"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592516_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9154" checksum="44C71A3B9D90908D" modified="2013-10-16" version="1">MYPEYYKDAPHFHRIGPEGPEYRLEIPNAKLDFTGTYSIIAKNCHGEAKAIISLQIMAKVLSVQRITIGLTCRNIDRFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA53</accession>
    <name>T1GA53_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEAT repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02061088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GA53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000098-RA">
        <property type="protein sequence ID" value="MESCA000098-PA"/>
        <property type="gene ID" value="MESCA000098"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001128_3_0_1"/>
    <dbReference type="OMA" id="CATQIDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 10 N-terminal" evidence="2">
        <location>
            <begin position="220"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Utp10/HEAT1 HEAT-repeats" evidence="3">
        <location>
            <begin position="1164"/>
            <end position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000098-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1424" mass="162594" checksum="A52350699EAE2853" modified="2013-10-16" version="1">MSTSLAEQLRRLAAPQTSILRDSKKRPSILFDAKEAATKDRQTIYDLAITGLHELTNLNPLFHQFEKTLFQKSSINMERSVETSEVNKQLDKTIKQFFYLASPYFMLHPTHLCLEWLIRRYHIHEFNRDEFIALVLPYHETAIFVKCVQLLNLKNPNDRWYWLKPIQKPGVPLPTSTIVNRAGSDNRIGQQSHMLQTAINFYCITVLGALESTKQITESHITTVLPSILKGLSSYVLDFTCAAFMIVTQIMVKCPALNEKLLNSWIARISVVPFENMRSEIILILSCMFQRQSTNMEFFPSEHLVKFVANRWVPESISELYAMGVNTKSLVEVLVKSCLKVIQGGNKEDAEVCKNFLEKLLGDVRFSAEDVKGVVSVTFNCYKPPEEKSTKPIKDGDVIELDSDEDESMDEGFHSWYCEFVQRLERQYPSEFDMIIKDLLRDQSVSNDVKQALKIVLSYRLDTATVSSNDATVFENLYHYNARNHKSSSMLADTILDRLKDDNPKVLTEVLKLPTSKLIEKVGQENFVNALAAIIEASQIKGGQFSSLVPKVISHLSKCKGNSLEVVLAALPLSKPIAGKQSADLKALPKIDSVKFDDVLNFNYSHWTPFKVSYYLTLLEVTIPKNISEEQSSKAFEKVEDLLKKLKVVYIEKDNEEDILASWRNNKIPLFLVLNFYTQMVKVSKKFSNTVYKLFATKAFTKDGLEKQDYVLSLKTMLEFVFPTAQQKLEFFANFFAYKEIDGFAVPPELSVISIRLFNLVLQGKDQEYEITDLLLLKIIISLCSEYSVIHLLGKSEEIQMDGEQLPLIISALMKKKSEKVISKLLGLLKDSSVDIYLKSKILNILKYVNSPEILTQIIPIAYESLNSEVTEGQLKFIEGPHVQILENVISRFDESCIEVVLDESKAWDFLKTLISKNMTFLGHKGNIVPISAMVIDIFDEDVYAKFPESMKGELLQSIVSSISSAGNNNLFTKGNKFLKKCGLNGKHVHQILEKMLSCTGEQSKKTKRRSVTDAIGTEVLNCVQWKEGITLLELMENKKKIENSSNTIPLLFEILKTCLLIQEQSLVEYSKQLILSALLHLCQKYQEDQRSESSNSQPSIVASVPCAIVYPQQVLHNIMDIFTFMGSSVVRHDDVFSFQIISNIIESVIPTIVKKNNKESVELVIPVFKVFADILLDVPEHRRLPLYTKLITTLGTGKYLWMFLAVVFESHVIHNSEDEGKKKGQPKARVENPLQRIDICLRIVKSFKPQEVLETCIQLMSYLKTLPEETNSEKPNKMEVDLTDTKLFDPDVRSLKQLRHYRYIILQFMSNLTSSADFIHNIALLSEEETKFMKPFYQNLIIKTLSYIPNVSSMSEKFEDSKYWKVCLTHCYDVLDNSISLLSPDMFVVVVNGLMKHKLLIIRKKVIDLLNNKLQHQGEYFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA96</accession>
    <name>T1GA96_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA96"/>
    <dbReference type="STRING" id="36166.T1GA96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000146-RA">
        <property type="protein sequence ID" value="MESCA000146-PA"/>
        <property type="gene ID" value="MESCA000146"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150417_0_0_1"/>
    <dbReference type="OMA" id="DVTYITI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06669">
        <property type="entry name" value="PDZ5_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000070">
        <property type="entry name" value="Multiple PDZ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051342">
        <property type="entry name" value="PDZ_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964">
        <property type="entry name" value="MULTIPLE PDZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964:SF20">
        <property type="entry name" value="PATJ HOMOLOG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="19"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000146-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="15333" checksum="276075744A3279B3" modified="2013-10-16" version="1">TKSVENVSGLALWTNDVTYITIEKTERGFGFSILDYQDPLDPDGTVIVIRGLIPGGSAEATNQIFPGDRLVSVNDSLLHGLSLDEAVSILKGIPLGFTKLGICRPLSSSDNNDSSPEDPDPDVDENDSLRSNSHSNSNTNSNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB81</accession>
    <name>T1GB81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB81"/>
    <dbReference type="STRING" id="36166.T1GB81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000503-RA">
        <property type="protein sequence ID" value="MESCA000503-PA"/>
        <property type="gene ID" value="MESCA000503"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF147">
        <property type="entry name" value="EG:BACR7A4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="32"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="241" mass="26624" checksum="F8E3942957F97DAA" modified="2013-10-16" version="1">MDEIKWNSNSGIVLKKLAPLSNYTSGSIEFKCGGTLIDLQFILTSAHCVDHINFVILGDRDISLKSSSNSRQYFNISKHIPHELYLPTMKYHDIALIKLSRPAMISENVRPALLPSIDGNKDRNDSIFIIAGWGAIGPYEGFEEKLRKVELKSYTNDYCKKVGSSGLPYGINETLLFCAGDENKDTCEGDSGGALVTNSSNLFTIFGIATSGQGCGLGVPGLYVNVFHYVNWIKETIRRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB90</accession>
    <name>T1GB90_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB90"/>
    <dbReference type="STRING" id="36166.T1GB90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000513-RA">
        <property type="protein sequence ID" value="MESCA000513-PA"/>
        <property type="gene ID" value="MESCA000513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_2_1_1"/>
    <dbReference type="OMA" id="AILMYPA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000084">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF140">
        <property type="entry name" value="FI02848P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588157" description="Peptidase M14 domain-containing protein" evidence="12">
        <location>
            <begin position="18"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="108"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="407" mass="46061" checksum="2C466AB63B778C70" modified="2013-10-16" version="1">MWTKTFCLLFCLSSALALKDYKILELFPSNDHQSDLLHKLSEDSKFDFIKLTKSEAQVLVSPESHKVISSLLSKYGIGYKVVNNDVLADAIKESEENESSQERMGTDCYRSHGEINNWLDELQAKYPQRVFVKQVGFSYERRPLKTITITNGDGRSGKKVIFVDAGMHAREWISPSTGIYVIQQLVENFEENKALLKDHDWIVMPMINADGYEYTRSDPKNRMWRKTRQPYKTCAGVDPNRNFDYMFGHAGTSTLSCMDTYRGPQPFSEPETSVLRDVLLSLKGRISFYLTLHSHGAYLIYPWGYDKVLAPNNDELQDVGQAGYDAIYAYSGRKYLVGNAAILMYPAAGASDDYAYARADARISITMELPSGGKQGFDPPPSDIKPYVEESWIGIVAMANRVIAKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBY4</accession>
    <name>T1GBY4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000775-RA">
        <property type="protein sequence ID" value="MESCA000775-PA"/>
        <property type="gene ID" value="MESCA000775"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1637351_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588176" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000775-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18381" checksum="5C09C63AA25A5A5D" modified="2013-10-16" version="1">MSGKQFTIYLVFILTRGILSQNGINSTSKTVPILQVSSKPTAKDCKITGFILDPQSNCKNYFTCTKNPITGRMEISEESTPCPYGLFDEDIQDCITDNCPSKCYNLNVIRNFCQTVARILQFIHISMKRIAESIQIAKQEKKLLWNVLPTNMHRFLGQAYCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC29</accession>
    <name>T1GC29_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC29"/>
    <dbReference type="STRING" id="36166.T1GC29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000825-RA">
        <property type="protein sequence ID" value="MESCA000825-PA"/>
        <property type="gene ID" value="MESCA000825"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1215988_0_0_1"/>
    <dbReference type="OMA" id="DRISHYH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF41">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007365883" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="20"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000825-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="26479" checksum="512ACB519BEED9A5" modified="2013-10-16" version="1">MKFVHVFAISLLVISVVSDEHTHLVIHGQGGGGLWMNTVGPYHNRQETYAYFSLPFCSGPKDRISHYHESLSEALQGIDLVFSGYEMDFKEDVPRKTLCMVELTKDKVETFSYAVSKEYWYQMYIDGLPIWGKVGEKNPSDNTLSIYTHKKFEIGYNGKQIVDINLNTEEKKVLAVGEKIEFSYEVTWKPSAVKFENRFDKYLDPNFFQHRIIKRWRHLAVLSGNEIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC41</accession>
    <name>T1GC41_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM129 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC41"/>
    <dbReference type="STRING" id="36166.T1GC41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000838-RA">
        <property type="protein sequence ID" value="MESCA000838-PA"/>
        <property type="gene ID" value="MESCA000838"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318296_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018801">
        <property type="entry name" value="TM129"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31322">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TM129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31322:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TM129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10272">
        <property type="entry name" value="Tmpp129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577252" evidence="6">
        <location>
            <begin position="27"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000838-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="24025" checksum="07AE57037D8BF8EF" modified="2013-10-16" version="1">PGFWRFAHSLCLFAVFFIFPLTYLHCVSNNWEKHPISIKMLPFSNSNGNFVDVASDISTEFRRNNIVVIKMNTTERLVVTDNWIIKISSLSIDFAHQSDSSLVLDKNETYKNPEDSSELVQMLSMKVTSMRSNVKEFRIRINASAFEDLQDRLSRPITVNPGFHLHKTVMDRFIEVFKEQVSQNPIYSTDITSDQCFACMISPPPLGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC53</accession>
    <name>T1GC53_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pellino FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC53"/>
    <dbReference type="STRING" id="36166.T1GC53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000851-RA">
        <property type="protein sequence ID" value="MESCA000851-PA"/>
        <property type="gene ID" value="MESCA000851"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590545_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008592">
        <property type="term" value="P:regulation of Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006800">
        <property type="entry name" value="Pellino_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048334">
        <property type="entry name" value="Pellino_FHA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PELLINO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098:SF2">
        <property type="entry name" value="PROTEIN PELLINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04710">
        <property type="entry name" value="Pellino_FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pellino FHA" evidence="1">
        <location>
            <begin position="92"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000851-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19762" checksum="B4E1238BA5B39257" modified="2013-10-16" version="1">MKRLTTRKGPEEDPITTKHSSPFATHLQEKVVKIQKSYFESTTKFVNIRYQVEQITFRRTNDYNYLLRNHKTVDCLIIVVWSHQNSTLPGKYNGYLPLGDRGRRRSKFVLYKRNEPNGVKKSKDYLVQSPQNSRAILDTNQHSISYTLSRNQAVIVEYKEDAETDMFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCB0</accession>
    <name>T1GCB0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02186415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCB0"/>
    <dbReference type="STRING" id="36166.T1GCB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000920-RA">
        <property type="protein sequence ID" value="MESCA000920-PA"/>
        <property type="gene ID" value="MESCA000920"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_3_1_1"/>
    <dbReference type="OMA" id="TIEWHIN"/>
    <dbReference type="UniPathway" id="UPA00796">
        <property type="reaction ID" value="UER00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033320">
        <property type="term" value="P:UDP-D-xylose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="14">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000920-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17950" checksum="9F11F20533B1D889" modified="2013-10-16" version="1">MHMNDGRVVSNFILQALQNKTITVYGNGKQTRSFQYVSDLVDGMILLMESNYTQPINLGNPVEHSIEEFASIIKASVGGKSEISKTEAVEDDPQRRKPDIARAKKYLNWEPKISLKDGLQRTIDYFKMELERSNQDFSELADGRLRDLDPRKISEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCK5</accession>
    <name>T1GCK5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCK5"/>
    <dbReference type="STRING" id="36166.T1GCK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001020-RA">
        <property type="protein sequence ID" value="MESCA001020-PA"/>
        <property type="gene ID" value="MESCA001020"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009579_11_6_1"/>
    <dbReference type="OMA" id="GTKQVMM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF185">
        <property type="entry name" value="DOPAMINE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="96"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="278"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="304"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001020-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="438" mass="49326" checksum="90AB4E842DA04D7D" modified="2013-10-16" version="1">MVTMSINTDHRLLSQAGTELFFNLNTNQTIFESYNVSDGQYNFIDGFTEIPPSFNTTELSYVPAHLDLIEYLESLSNNKASLLAFLFLFSFATVFGNALVILAVIRERYLHTATNYFIGSLAVADCLVGLVVMPFSALYEVLDNTWFFGTDWCDIWRYWAITDPFTYPMRMTTKRSMFLICAVWVCSSAISFPAIVWWRAARATEMPAYRCTFTEHVGYLVFSSTISFYLPLVVMVFTYCRIYRAAVIQTRSLKIGTKQVLMASGELQLTLRIHRGGTTREPSTAHHQLTHHHRGSSSHHHFNHPSSGSASGALPGTLGNHMGGTTTSTPEEPDDEPLAALHNNGISRGHRQMGKNFSLSRKLAKFAKEKKAAKTLGIVMGVFIVCWLPFFVFNLLSGFCVQCIAHEEIVSAVVTWLGWVNSCMNPVIYACWSRDFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCM1</accession>
    <name>T1GCM1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02107115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCM1"/>
    <dbReference type="STRING" id="36166.T1GCM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001039-RA">
        <property type="protein sequence ID" value="MESCA001039-PA"/>
        <property type="gene ID" value="MESCA001039"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_1"/>
    <dbReference type="OMA" id="RIVKTFW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000052">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="11">
        <location>
            <begin position="13"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="10">
        <location>
            <begin position="186"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="12">
        <location>
            <begin position="419"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001039-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="547" mass="60848" checksum="1371B5E468D7FF8E" modified="2013-10-16" version="1">MNVAEKDFLYGHVYGVSGPVVVAEDMSGAAMYELVKVGHFELVGEIIRLENDMATIQVYEETSGVTVGDPVKRTGKPLSVELGPGIMGSIFDGIQRPLQEINRVTNSIYLPKGVNVPSLPRDKLWEFSPEKLFVGSHITGGDVFEVVLETEYQGEKTEHTMLQVWPVRQPRPTTEKLRANHPLVTGQRILDSLFPCVQGGTTCIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEINGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSSSRWAEALREISGRLAEMPADSGYPAYLSARLASFYERAGRVTCIGNPVREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYLNALDDYYEHNFPDFIALRNKMKLILQDEEDLSEIVQLVGKGSLAEKDKITLETVGMLKNIIRFYDMAIQCVETTSHSDDKITWQIINETLYSTVMYRLTQMKFANPNKGEQKVLAEYDQIYEDMVQAFRSFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCM4</accession>
    <name>T1GCM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082730">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCM4"/>
    <dbReference type="STRING" id="36166.T1GCM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001042-RA">
        <property type="protein sequence ID" value="MESCA001042-PA"/>
        <property type="gene ID" value="MESCA001042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451119_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="20921" checksum="5D95A55F9BA86D9F" modified="2013-10-16" version="1">PIVPIEETSKVSEENNVLKENIPETTILDKSVEEVSVLNENTTETEKLTENTEIISGEAQVKKKRKRRKNELAAIVADQLLESFKEVDKSRIDDLKMLENLAYEKSEDLLLTGMRLTPTTKRKQQSESLPATPTPQKKAVPRKKSQKEKPLETVKPTTKTRSTVIGITGAGWLVGRGQSITTPIENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCV6</accession>
    <name>T1GCV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02035150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCV6"/>
    <dbReference type="STRING" id="36166.T1GCV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001129-RA">
        <property type="protein sequence ID" value="MESCA001129-PA"/>
        <property type="gene ID" value="MESCA001129"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011722_1_6_1"/>
    <dbReference type="OMA" id="LTATWCH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000056">
        <property type="entry name" value="Potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF14">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="11"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001129-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="24673" checksum="E5DEE3B55EF30CE8" modified="2013-10-16" version="1">MVVLSVGSNGQVFATSAIRGIRFLQILRMLHVDRQGGTWRLLGSVVFIHRQELITTLYIGFLGLIFSSYFVYLAEKDVVDADGKNDFSSYADALWWGVITVTTIGYGDAVPKTWMGKIVASCFSVFAISFLLYQRGFALKVQQKQRQKHFNRQIPAAAMLIQCLWRCYAADKSFNSEATWLIHVKDGESTSINANNSLNAVLPTQLGKVDRCPQECHSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCW7</accession>
    <name>T1GCW7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146332">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001140-RA">
        <property type="protein sequence ID" value="MESCA001140-PA"/>
        <property type="gene ID" value="MESCA001140"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278941_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001140-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="24444" checksum="F08AA03AC13912C9" modified="2013-10-16" version="1">MINHEITQEQYKTYKCRTVKEKIYSNPNSMLLPFTIHDKKLENLVRKSKVRNWQPPNLSTVVVVPKSSWSSSFNFFNKSSFTEVSGIRSSIPGLKRESGIHAIHPMKVLSSTFEGLETSEISLLLKELFFKAQRKEELYIVVSHERSVFCPFGGSEAAGNLVCNGGCNIIMQCNAMPSFHAFHSKDSLYAMKTMKNRLEILNQATTTSAMANAQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEA6</accession>
    <name>T1GEA6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brinker DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEA6"/>
    <dbReference type="STRING" id="36166.T1GEA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001664-RA">
        <property type="protein sequence ID" value="MESCA001664-PA"/>
        <property type="gene ID" value="MESCA001664"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_687516_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018586">
        <property type="entry name" value="Brinker_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09607">
        <property type="entry name" value="BrkDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brinker DNA-binding" evidence="2">
        <location>
            <begin position="40"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001664-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="401" mass="43659" checksum="1BBE01E8B6E1878D" modified="2013-10-16" version="1">MNSNSQVPINSTKSSIVVGAAAAVAMNASSTDTTPKAKMGSRRIFKTEFKVQVLDSYWNDNDCKGNQRATARKYGIHRRQIQKWLGNEKSLREQLKNQNTNNVVKPHQFHNVAYGNVAAAAVVAASAVKVSSTSPQTASKLMSLGQARHCVAPTSTTSLSPSSTSSSSSLSLSPSAVGTNTSTAITPIRPIPHQHPHTPVTIVPPTHFTQQHYHYHHSQLPHFMPPEQAPAKSSTSTSAPSVLAVDLTCRKRQNQSVADEDCTPEKVQKIKEENADDDDEEVDVDVEVDDGIEVQVAKPIKLFKPYLLDEEEENKDRSPIFPSSPSSLGAFSPAAACPKGSPVSVSGYESSSSTSTFGEYPTYNYSIDFKVNNYPTARSDNHSHVQRWLDHDANLRQGIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEC4</accession>
    <name>T1GEC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PBZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HPF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02034565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEC4"/>
    <dbReference type="STRING" id="36166.T1GEC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001684-RA">
        <property type="protein sequence ID" value="MESCA001684-PA"/>
        <property type="gene ID" value="MESCA001684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053037_0_1_1"/>
    <dbReference type="OMA" id="HKELHML"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072572">
        <property type="term" value="F:poly-ADP-D-ribose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019406">
        <property type="entry name" value="APLF_PBZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019361">
        <property type="entry name" value="HPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13386">
        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13386:SF1">
        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10228">
        <property type="entry name" value="HPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10283">
        <property type="entry name" value="zf-CCHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBZ-type" evidence="7">
        <location>
            <begin position="3"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="433" mass="50189" checksum="FA36503E6660545A" modified="2013-10-16" version="1">MDKENCKYWDKCYQKNPAHIAKYNHPSKTNDSPDEAGQSSPKKQSTSPNSRKRHHDESPKTSPSKQEDKIQSDQTREEMDITEEYNEVMKNLEGKNFLEIYERRVKLSAKAEYLELLKTNEFIRYKFLVEMPNDFYEFWEFCKTISSETPENAFSSVGLKLVGPFDFLAGKFHKHIFMNQYHRGECAFLNVAFVSNLRDYIRHWRFFYDPAEFQTVFVKEKSSVHYGYWRDSPQDKENLLIARNETAKGCEFDFVAGNLFEALNYFLDNDFAKNPFNQASIKKIKKGMEDFLVGKSFGTLQETKKVRDKQVVAKTFHKCGIVVPYDRKTQLGYRTLMESDATIKKYLKMLETGDNLDVVMEKLQPIISAASIAADECDFGAGLELGIDLFCSGHTNLHDIVRSLLIPAYSLLKRPQFIAISKAHMDPERKAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEC5</accession>
    <name>T1GEC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02034565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEC5"/>
    <dbReference type="STRING" id="36166.T1GEC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001686-RA">
        <property type="protein sequence ID" value="MESCA001686-PA"/>
        <property type="gene ID" value="MESCA001686"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_0_1"/>
    <dbReference type="OMA" id="GYTPCFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000002">
        <property type="entry name" value="Serine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="190"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="286"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="359"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="11">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001686-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="463" mass="52057" checksum="F293759A3F4F52BF" modified="2013-10-16" version="1">MVLDLDLFRADKGGNPEKIKENQTKRFKDVGLVETVIAKDDEWRKRRKEIGEKMKKKEPVGDANEEVPDAIKADVTSVTSEVLKTLTVNQIKKVRLLLDEAMVENERLLVEAESQRNTALREVGNHLHESVPVSNDEDENKVERTFGDCTTKGKYSHVDLIVMIDGMNGEKGAVVSGGRGYFLTGAAVFLEQALIQHALHILHDKDYTPLYTPFFMRKEVMQEVAQLSQFDEELYKVVGKGSEKADENVPADEKYLIATSEQPIAAYHRDEWLPEASLPIKGIFRVHQFEKVEQFVLTSPHDNKSWEMMDEMIGNAEQFYQTLGIPYRVVNIVSGALNHAAAKKLDLEAWFCGSGAFRELVSCSNCLEYQARRLLVRYGQTKKMNANVDFVHMLNATMCATTRVICAILETHQTETGVKVPECLKMYMPAKYRDEIPFVKPAPIDLETAAAAKKGKGGKGQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEW7</accession>
    <name>T1GEW7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02119763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001890-RA">
        <property type="protein sequence ID" value="MESCA001890-PA"/>
        <property type="gene ID" value="MESCA001890"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576594_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001890-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9476" checksum="709591B75B67BD28" modified="2013-10-16" version="1">MDGLRLFNLNLRNFSFKTHKFPINIFDETKSTYLTKPSDSGDLSELEQSKIIQITGEIPVPANIFRFFKEKIIDDFITNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF96</accession>
    <name>T1GF96_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002031-RA">
        <property type="protein sequence ID" value="MESCA002031-PA"/>
        <property type="gene ID" value="MESCA002031"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078928_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002031-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13905" checksum="E988B4EF6879C946" modified="2013-10-16" version="1">HKRKVYILQAGFENNSQTVSQGKFSFYADKDNFLIAFFCCCIPLIHFQRLEEADGICLNVRRYKRKRKEENYRREIEWEASNLMEMGFIARDARVVGDERPFLQCAALSCRFSHIAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFA5</accession>
    <name>T1GFA5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="13">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="13"/>
    <dbReference type="EMBL" id="CAQQ02389586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFA5"/>
    <dbReference type="STRING" id="36166.T1GFA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002042-RA">
        <property type="protein sequence ID" value="MESCA002042-PA"/>
        <property type="gene ID" value="MESCA002042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014329_1_2_1"/>
    <dbReference type="OMA" id="VVRTENH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000003">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000001">
        <property type="entry name" value="Putative Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21292">
        <property type="entry name" value="EME1-MUS81_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="14">
        <location>
            <begin position="134"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="369" mass="42442" checksum="5950A64C291F783A" modified="2013-10-16" version="1">MSTQRIQIRLKDPNPVFTKWLEKWYQHAKEKNSKSQTNLKTALESLKKFPLELKSGKDCYILEGFGAKLCEMIDQELKTYQNQPPSTFESDVKEVIKKVKKTASTKSKAAQKAVAQQSQPLQQVVMNSGTFRIILLVDTAETSGKSKKDLDDTRLFLESFKVQYEVRRLSVGDFAWICRDSSGNEFVIPYILERKRMDDLAGSIKDGRYSEQKYRLKSSGIPNLIYLIEDHKNNDRLGLPLGNLKQAAVNTQIHSGFQIKHTRTHKDSMMYIKELPNLNKQDYVIGMLSFSDFQNSTAKNKGIQIREIFIKQLIQLKFLTTEKAIAITEIYPTPRALLEAYDECDSIEEKERRCLTVRDLNTITASYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFJ8</accession>
    <name>T1GFJ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 23</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02013781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFJ8"/>
    <dbReference type="STRING" id="36166.T1GFJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002142-RA">
        <property type="protein sequence ID" value="MESCA002142-PA"/>
        <property type="gene ID" value="MESCA002142"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2378723_0_0_1"/>
    <dbReference type="OMA" id="XERLLEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021629">
        <property type="entry name" value="Mediator_Med23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12691">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12691:SF10">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11573">
        <property type="entry name" value="Med23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002142-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10889" checksum="35F310F02C613199" modified="2013-10-16" version="1">PTHALIETYSRLLVYTEIESLGIKGFLGQLLPTVYKHQAWGILHTLLEIGSYRIHHIPTHYRVQLLSNLHSLASVPPSNKMQLNLWSSNLLRFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFU5</accession>
    <name>T1GFU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02094958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFU5"/>
    <dbReference type="STRING" id="36166.T1GFU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002245-RA">
        <property type="protein sequence ID" value="MESCA002245-PA"/>
        <property type="gene ID" value="MESCA002245"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_11_1"/>
    <dbReference type="OMA" id="LITFWMP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000325">
        <property type="entry name" value="Transporter, major facilitator family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF279">
        <property type="entry name" value="VOLTAGE-GATED PURINE NUCLEOTIDE UNIPORTER SLC17A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="48890" checksum="19F26BA3EBF06470" modified="2013-10-16" version="1">MQVEKLKYSLLRDSSDTQNLWSRSEKRQWFLTLIFGTCMLYSTRTTMPLLIPAVAKEQKWSKTDCVIGGYVSDRFGGQKVILLAALGWSLITFWMPSIIYAGPKYFKNNSISFIVTIRILNGAFQGVHFPSMISLTSQFLWVVNIRFSDGNSPHWNSRILCPGLLRMVNGISSNRTLGLSGTLILRYYTMSVNRNRVINVFNPSRMCTTKGGPDMEPVPWLKLFTRLSFWACVLTHACEMNCFFVLLSWLPTYFHDGFPQAKGWVVNMIPWLALPPCTFFAKYLSSYLISKEYSITTVRKLIQSLCFLSQNIALFCLSRTNSFQTALICMTVIIGGSGFHNNAVTVNPQDLAPNHSGSVFGLMNTVGAIPGFLGVYLAGHILELTQSWPVVFSTAAGINLVGWIVFVVFGSSEPIHKRRLFYLQNRGFIKLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFX9</accession>
    <name>T1GFX9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFX9"/>
    <dbReference type="STRING" id="36166.T1GFX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002281-RA">
        <property type="protein sequence ID" value="MESCA002281-PA"/>
        <property type="gene ID" value="MESCA002281"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_405061_0_0_1"/>
    <dbReference type="OMA" id="SIRETWW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2140">
        <property type="entry name" value="Beta-1,3-glucan-recognition protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031756">
        <property type="entry name" value="BGBP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043030">
        <property type="entry name" value="BGBP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF31">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15886">
        <property type="entry name" value="CBM39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51969">
        <property type="entry name" value="CBM39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577319" evidence="6">
        <location>
            <begin position="20"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="CBM39" evidence="8">
        <location>
            <begin position="22"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="TRUD" evidence="7">
        <location>
            <begin position="401"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002281-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="679" mass="77487" checksum="93C6D98F3157AF7D" modified="2013-10-16" version="1">MIFILKLLIITLIIHGVFSATYVIPAPKVELLSPKGFQVSIPADVDIKLFAFHGSIGTPLNSIEEGDFNGEITEPENGRFTYVNTKNTLESGDVLYFWVHVQKGNLGYKKISQASKILPSGEPSETYTVGDEPESPPKTKKPTTTFIFPSTAVTGSNNNQVDLDAFEAILSLHRSCLPNSFTGGCLPIFPEILDLTSTKEEFKDVVTEDIWSKIEEMVKFNPNKSSESEFPAPIDIKVNDLSKEKRTQIHKLVKQTFNNKIISSTVNPPDSEDKFIRISRAKGGKGDNRNRWTWDGDYVHFIVRKENIDTLQAASEIANALKLRPSQVNYAGTKDKRGITTQMLCIKKREPQQIHRSCRHIRNEVLKLGQLKGNQFKIALRHLKGDQNEIEKSLENIRDFGFINYYGLQRFGNCASIPTYEVGIAILKADYKLAAELILKPREDDLSFLKSIRETWWKNRNSSEALGMFQYKNNKFIEYRLLEGLAENNETGYANALRKIPRNMLLLYIHAYQSLIWNRVASRRIKEFGMKLIPGDLVYNDSTEIEEILDSVEEDEEDQQPKESIFKQKVKKLTEEDISSGKYTIFDVVLPQPGFDITYPENEVGKWYEEYLAENDLTSEKLKNNVKTFSLPGAYRKMLVKPGNMTWEFKTYNSPTDNLIETRFEKATVCENGQLKALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG58</accession>
    <name>T1GG58_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02018208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018209">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002367-RA">
        <property type="protein sequence ID" value="MESCA002367-PA"/>
        <property type="gene ID" value="MESCA002367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429587_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10774" checksum="F28058B9DB1D2047" modified="2013-10-16" version="1">MSRIQKRKGIHGYRRHGQRDQNIDLENNVHPRLEHIFISKKCNLPTFKHQLITNRPLQKNLIYGLSICVLIHQDICVLRDFVLKGEKVFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGC8</accession>
    <name>T1GGC8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein 21A/21B fourth ARM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGC8"/>
    <dbReference type="STRING" id="36166.T1GGC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002444-RA">
        <property type="protein sequence ID" value="MESCA002444-PA"/>
        <property type="gene ID" value="MESCA002444"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1410300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056836">
        <property type="entry name" value="ARM_TT21_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040364">
        <property type="entry name" value="TTC21A/TTC21B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699">
        <property type="entry name" value="STI2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 21 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25068">
        <property type="entry name" value="ARM_TT21_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fourth ARM" evidence="6">
        <location>
            <begin position="18"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002444-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="21675" checksum="0CAE4B7FDE26D66C" modified="2013-10-16" version="1">MPGRGTLAQNSLKNPRISNETDSSALQIRTKQLLLLARVREKSGNIPGSLNTLKEARENQYKLQKRISIEQPGSLLEHYKVMSKICTLLAEQSISLRENEQALQYYKEALKFSPEDVQILCSLGRLYMQMNLMDLCRDICSQILRIDSNNETASVMMAGGGGLMSSQAIFLRKVSITEQLQCIIIVLGLVVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGK5</accession>
    <name>T1GGK5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGK5"/>
    <dbReference type="STRING" id="36166.T1GGK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002526-RA">
        <property type="protein sequence ID" value="MESCA002526-PA"/>
        <property type="gene ID" value="MESCA002526"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046192_0_0_1"/>
    <dbReference type="OMA" id="FYFQQNN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002526-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="364" mass="41298" checksum="F0A1D7A7CC01C758" modified="2013-10-16" version="1">MFEKPQFEGASYLMALDLGYNKLTEIPRNIFLGADNLRTLSLEFNQIKSIHNQSFLALKELQSLSLEGNELEYVTNTMFAPLRKLEVLNLARNKLTVVPVGIFNRNTHLQHIKVGFNMLRSVDANVYRTLTKLKTLDYSGNIMPHVVLNFTRLENFVATDCEIMRLDIFGTVIDINLRNNSLEFIPAFPDAGNLTKLDISSNPLAKIDTNTFRKFTGLKRLNLSSSNLHELPDGSLETFYFHQNNWNCDFLEHLMNSFVKRGEIVFQEDTIAPELVDDFRDGIACWYESNGYTYRISKKFGVATNPRGGMEGGGGGPSGRGGGSCDDSVIELSIVRNDIKHFFEVIEKKFIKLSKTLDDIQMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGY2</accession>
    <name>T1GGY2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002663-RA">
        <property type="protein sequence ID" value="MESCA002663-PA"/>
        <property type="gene ID" value="MESCA002663"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349064_0_0_1"/>
    <dbReference type="OMA" id="MVEHESW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002663-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11554" checksum="3E6B77BEE3AA2FEE" modified="2013-10-16" version="1">MNHEVAAALRFLVHLDPTKNNMETTAWFLNKVCHWHDLVTSRSRALSLSLFNKEKNQSALLFLKEFQKIINRKETKSNSFTKRNFPYNRILYEIIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH27</accession>
    <name>T1GH27_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390955">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH27"/>
    <dbReference type="STRING" id="36166.T1GH27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002710-RA">
        <property type="protein sequence ID" value="MESCA002710-PA"/>
        <property type="gene ID" value="MESCA002710"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120649_0_0_1"/>
    <dbReference type="OMA" id="FRTDYAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576937" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="72"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="18614" checksum="89F4617BA4B908F6" modified="2013-10-16" version="1">MRNLWLPIAFCILFLTHFSDQQRNRGQHKYNFTGKKPVVIQHRTQDAVNYYDHENGAKIIKSSHFELDYHLGRKITFFCMAEGSPRPTITWFKDGAELYQHRFFQVHESMVGENIIKSKMEIDPTTQMDAGYYECQADNLYAIDKRGFRTDYVMINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH89</accession>
    <name>T1GH89_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02037119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH89"/>
    <dbReference type="STRING" id="36166.T1GH89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002779-RA">
        <property type="protein sequence ID" value="MESCA002779-PA"/>
        <property type="gene ID" value="MESCA002779"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006332_9_0_1"/>
    <dbReference type="OMA" id="HVFTRAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004423">
        <property type="term" value="F:iduronate-2-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16030">
        <property type="entry name" value="iduronate-2-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035874">
        <property type="entry name" value="IDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45953">
        <property type="entry name" value="IDURONATE 2-SULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45953:SF1">
        <property type="entry name" value="IDURONATE 2-SULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="52"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002779-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="511" mass="59525" checksum="56E33665C38C80E5" modified="2013-10-16" version="1">MVASVIEVFAKVKRTSNLSSKFQMNRSTLAKLFLVFAVFSLKITPTPSKKLNVLFVMFDDFRPAISGYGDKLSKTPNLDWFMQKSFHFTNAYSQQSLCAPSRNSLLTGRRPDSTHLYDFYNYWRNFPEVGNFTTLPEYFKNHDYLTHSVGKIFHPGISSNFSDDFPYICPDGTGYLRKNLICPVKLKTQPMKTLPDIESAKEAVRFLSKPKEKPFFLSVGFFKPHIPFRFPKYFLNKFNKDDFKNYTQDIFKPLDMPHVAWNPYMDIRKRDDFKNLNISFPYGPVPEYQRISIRQAYYASVSYVDDLFGYIMKNVNFNDTIVLATSDHGWSLGEHAEWAKYSNFDVALKVPLIVYSPEFKLDSPKKVENIVELLDIFPTLADLAHLSPLKKCTRKAEKTCGEGNSLYSLLQSGNSKYAKNFALSQFPRPSEFPKRESDKPRLKHIKIMGYSIRTTYFRYTLWIKFNHKSFTKDWNYVYGEEMYDHRADKDEEHNLVGNRKISILELSLGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHA4</accession>
    <name>T1GHA4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHA4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002794-RA">
        <property type="protein sequence ID" value="MESCA002794-PA"/>
        <property type="gene ID" value="MESCA002794"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906668_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002794-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7243" checksum="E62D2245E5C4F8DC" modified="2013-10-16" version="1">MICQVPCLKREQNCCPNFLLPLFCHFEFQSKNNINGFEGQGVKVNSISKDIDYMHTRLINEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL7</accession>
    <name>T1GHL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002924-RA">
        <property type="protein sequence ID" value="MESCA002924-PA"/>
        <property type="gene ID" value="MESCA002924"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3351621_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002924-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="37" mass="4216" checksum="8DEF1B52DB9C6287" modified="2013-10-16" version="1">MPYLSGFCISRVMYILSLLFTLILIAETTGTGVNYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHM8</accession>
    <name>T1GHM8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHM8"/>
    <dbReference type="STRING" id="36166.T1GHM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002935-RA">
        <property type="protein sequence ID" value="MESCA002935-PA"/>
        <property type="gene ID" value="MESCA002935"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867439_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002935-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15356" checksum="645E9FEDDBEB5496" modified="2013-10-16" version="1">MSFSQVARSLSKSANLFRSGRILAGTVRPQIRMISQSFAPVSYTITSNKNFQSLRLYSSEGGKKPSLVEITERVLKVVAAYDKVTSSNNQVSLWQLQSTRLYSGKPPLNLKLIEERVVLVLNLYDKIDPAKLLNLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHZ2</accession>
    <name>T1GHZ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium-dependent multivitamin transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02182688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHZ2"/>
    <dbReference type="STRING" id="36166.T1GHZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003058-RA">
        <property type="protein sequence ID" value="MESCA003058-PA"/>
        <property type="gene ID" value="MESCA003058"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_11_1_1"/>
    <dbReference type="OMA" id="MAQFFNS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003058-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="58169" checksum="9E4B70FF1D90074A" modified="2013-10-16" version="1">MATFSSYDYAVLAVTLISSSLIGVVYGFLEKKHKTTRNYLLADRSMKVIPVAFSVTASYISAISLMGTPSEIYQFGSIILYAMLALVLATPTIAYGILPVFMNMGATSIYEYLELRFGVLLRVTVSLIFTFQQILFMAIAMYAPALALETVTGINRFLAISVIGLVCTFYSTIGGIKAVIATDILQGILMVFPLLGAFIYATIETERANQVQVQRFSTVEGLRQSQKCVWWNLLFMTVLQGLIFLGSIAIFWYYHNCDPLLEGRITSRDQLLPLFIMDTMSHIPGLPGLMMSAIFCGALSSVSSVINSMSAIVLEDYLKPLNKVCCKVELTARLTLLTTKAISLTLGIIFTASAFLGDCLGGLLEAALTTSGVVAGPILGVFLLGMLTMNANHIGTLFGLVIGLCFASFMAFAQPKATLPILELSTESCINFEPQEYFVESEEEYFWFYNVSFMWYSVIGFLATFICGYFLSWILYWCRLQSNEKLYIENKNFNAMLLSPVFVETLVFFGWRKYEGSDEDLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI05</accession>
    <name>T1GI05_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02146527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI05"/>
    <dbReference type="STRING" id="36166.T1GI05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003072-RA">
        <property type="protein sequence ID" value="MESCA003072-PA"/>
        <property type="gene ID" value="MESCA003072"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_1_1"/>
    <dbReference type="OMA" id="RMHIERW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="13">
        <location>
            <begin position="6"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003072-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="21864" checksum="28E67F091A4E873E" modified="2013-10-16" version="1">KEWKGSYEVILVSDGSQDKTVELGLKYSKKYTSAKFRVLDLLENRGKGGAVRLGMLSTRGKFCLFADADGATDFKDFSKLNELMNELTGNDFNTEAIVIGSRAHLEKESIATRSFFRTVLMHGFHFLVWFFTVKSVKDTQCGFKLLSRSAVQKLFTIMHVERWAFDVELLYLAEKMKIPIKEVAVNWQEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI95</accession>
    <name>T1GI95_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02109037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003164-RA">
        <property type="protein sequence ID" value="MESCA003164-PA"/>
        <property type="gene ID" value="MESCA003164"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1483640_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003164-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="21032" checksum="E115218249EF7EAE" modified="2013-10-16" version="1">MGLCTLHVTPEKYFNGISLGHAYSIHTCLCLQKWIGSEDCSNARRKMFAPFIFPVFRAAEIYATGTSKAKDYQKYSLQPPYLQWFSFVYNEGYLCDRNCGSVSEDSWLSIFPISEITAKKYTKKSYICNLLSKVLKLYNSELMFHNTLSYAKNFKCFSTEKDVTYYTLPIHDIMDGHSEVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIV7</accession>
    <name>T1GIV7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GIV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003392-RA">
        <property type="protein sequence ID" value="MESCA003392-PA"/>
        <property type="gene ID" value="MESCA003392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_5_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46331">
        <property type="entry name" value="VALACYCLOVIR HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46331:SF2">
        <property type="entry name" value="VALACYCLOVIR HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="46"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="200"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="269" mass="30212" checksum="BEC5DD324701DD88" modified="2013-10-16" version="1">MLRNISGFSKIFLRRLSSQTPIEKKVKIPSGHEVNVVQSGNGQKSVLLMPGALGGAWSDFRPQIEQLPQLIPNYTIFAWDPPGYGKSNPGKRYFPVDFYNKDSQWAHELMQELGISTYSIIGWSDGGVSGLISAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGVDGFPKLWSAWVDGVLDIIKKNGSNLCKEEILKIQAPVFILHGKKDPMVDGEEHMPYLKEKIKNVTYYEFPDGKHNIHLKYAKEFNELVANFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK72</accession>
    <name>T1GK72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02012814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02012815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02012816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK72"/>
    <dbReference type="STRING" id="36166.T1GK72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003890-RA">
        <property type="protein sequence ID" value="MESCA003890-PA"/>
        <property type="gene ID" value="MESCA003890"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880594_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF5">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="25"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003890-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15574" checksum="15035CDCF9343321" modified="2013-10-16" version="1">AKDDDGSSVKSALDGSSLHGLSKKQRKARTAFTDNQLQTLEKSFERQKYLSVQDRMELANKLNLSDTQVKTWYQNRSLVTTSVIFSEKIRFRTTSFHMKNWKWKSMCSGVTKELGKKEENIHLNAVEGPIAPHIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKH1</accession>
    <name>T1GKH1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="4">SDH assembly factor 2</shortName>
            <shortName evidence="4">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with the flavoprotein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SDHAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKH1"/>
    <dbReference type="STRING" id="36166.T1GKH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003994-RA">
        <property type="protein sequence ID" value="MESCA003994-PA"/>
        <property type="gene ID" value="MESCA003994"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103054_0_3_1"/>
    <dbReference type="OMA" id="TFAGKYL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003994-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="18635" checksum="CACCBA98DBE52B52" modified="2013-10-16" version="1">MLRQILKTNSTYQRTAFLARYFSSDKNGPKTINNADIPIVDFEDPEYLPLPQYPEKVNEPLEKRKQRLLYQSRKRGMLENDLLLSTFAARYLKDFTATQTEQYDKLINGVTNDWNIYYWATNVRETPEEYNNEIMDMLKKHTMNENKESRVRQPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKQ2</accession>
    <name>T1GKQ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004079-RA">
        <property type="protein sequence ID" value="MESCA004079-PA"/>
        <property type="gene ID" value="MESCA004079"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906660_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004079-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7589" checksum="C506716C92C8B4FB" modified="2013-10-16" version="1">MKYLIFHLFRNYKFQRHQKTVIPLRKLDPTSNRLLPPGGFWAKLEEIFFELVSGTKFCNFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL17</accession>
    <name>T1GL17_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02200224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL17"/>
    <dbReference type="STRING" id="36166.T1GL17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004203-RA">
        <property type="protein sequence ID" value="MESCA004203-PA"/>
        <property type="gene ID" value="MESCA004203"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867609_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF81">
        <property type="entry name" value="GH12661P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="321" mass="37382" checksum="1D25B3812D1B071B" modified="2013-10-16" version="1">FNRDGFYVIEDFLTPEETEELHKTGKTLCIEAPKENRKIFSTTNSESNQNRENYFLESGDKIRYFFEKDAVGQEGELLVDPLHALNKVGHALHTDHPTFNKFTFSNRVKEICWQLGYRRPAVPQKNAVLEFLILETEMLCRKLITSAQKRVFPSSILIFRGTKTKVVPNLHGAETKVVVPEKTLINKFFGKYLLITNTVSSGLLMIAGDVICQEIEFRRGELKKRYDYKRIWQMFLVGSLQGPMHHWFYGWLARIMPEATVKNAVKKILWDQFVMSPACILMFFYPACLLENKPFHEATKEIKDKFLTVYVVSKKLISFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM65</accession>
    <name>T1GM65_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004633-RA">
        <property type="protein sequence ID" value="MESCA004633-PA"/>
        <property type="gene ID" value="MESCA004633"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199653_0_0_1"/>
    <dbReference type="OMA" id="YGGGYWP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588401" evidence="1">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11023" checksum="5810522D5A2E3EC8" modified="2013-10-16" version="1">MNKLIVCALFLSCFALVLAVPRPAPQRAAVAESKIEDLEAGDSEKDDLKGSASYGYGYYGGLGGYYGGGYYPYHGAYGYPYYSYGLGYPYYGGYGLHGHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMN0</accession>
    <name>T1GMN0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YPP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GMN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004806-RA">
        <property type="protein sequence ID" value="MESCA004806-PA"/>
        <property type="gene ID" value="MESCA004806"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_750716_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="298"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004806-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="369" mass="41921" checksum="8F9154B669FC8641" modified="2013-10-16" version="1">MIHCHHFCPLXLCYESLGLFHEGLHHSKQALAKXXXXXXXXXXXXXXXXXXXXXXXXELKGLRPSRSQLYVGIGYQQIANQSNLNSDREKYHKLALEAFQKAVEDDENDHLSAYYLALEYALLNNXXXXXXXXXXXXXXXXXXLEYALLNNIPEAMHNIRCALALRAEHSPSLHLFTLILTATEKHFEALNVVEDALEEFPENLNLLHVKAHLELYMEEPHSALKTVQSMLNIWRDHYESQILNGNGTNGGGILALTENADISERHSDTKTDVYLAVDQPEEASNCINEASQIYPLAYQIMLTRGQYFVHIGKLEDAKENFFNAVAANPCNTESLRALGEVYYTLGDYRMAEKFTRDAAKYNPDCYKSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN68</accession>
    <name>T1GN68_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IMD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN68"/>
    <dbReference type="STRING" id="36166.T1GN68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005011-RA">
        <property type="protein sequence ID" value="MESCA005011-PA"/>
        <property type="gene ID" value="MESCA005011"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1242806_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF4">
        <property type="entry name" value="FI21477P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005011-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="24296" checksum="B97B53644D53ECA6" modified="2013-10-16" version="1">MIEERTRYCAFVNLLQPVVKEECEVMFELGHLQEAMQSIAIVTKDPGTLPQASEELIMDAKSNMNLYPESPGGNSGSHGCSNSLGSRKSSVCSISSINSSGSSSSPGHHHYQRSISQFISPSIRLKPGESSDSVDQIYEDSIQELNNLIGELDSFQREHEAKEKLRMYSNSSSSSNNGHHLNQIPVNGNNGVFGTSDAESDKISLGSLNYSTHSTKYSDSEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GND7</accession>
    <name>T1GND7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02127647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GND7"/>
    <dbReference type="STRING" id="36166.T1GND7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005084-RA">
        <property type="protein sequence ID" value="MESCA005084-PA"/>
        <property type="gene ID" value="MESCA005084"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2446472_0_0_1"/>
    <dbReference type="OMA" id="WIGRFIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577043" description="Ion transport domain-containing protein" evidence="12">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="13">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005084-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11822" checksum="17B43BACDBAC2653" modified="2013-10-16" version="1">VVLVFILSIASLIIYFIDASSEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGRFITNINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPI9</accession>
    <name>T1GPI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02103557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPI9"/>
    <dbReference type="STRING" id="36166.T1GPI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005521-RA">
        <property type="protein sequence ID" value="MESCA005521-PA"/>
        <property type="gene ID" value="MESCA005521"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533972_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005521-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9286" checksum="E9DB519EB481C976" modified="2013-10-16" version="1">IYNQAKNKVVDIVQPHLNKVCALRVAGQQKASSLKDLSWQKANEVLATQYGSMAVSGVDSTSALAERLLDYYFPKADNDIEDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPV6</accession>
    <name>T1GPV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exoribonuclease phosphorolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPV6"/>
    <dbReference type="STRING" id="36166.T1GPV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005651-RA">
        <property type="protein sequence ID" value="MESCA005651-PA"/>
        <property type="gene ID" value="MESCA005651"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433186_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="5">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005651-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="9875" checksum="BEBE0DDDCA8A2813" modified="2013-10-16" version="1">RLDGRKATEYRSVDIALGSDATCVVSLGATKVMAHASCELVQPKAFRPNEGILTISVHLDNQGPDDSEHISNVDIVCLNRILEKLLKDSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQG2</accession>
    <name>T1GQG2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005867-RA">
        <property type="protein sequence ID" value="MESCA005867-PA"/>
        <property type="gene ID" value="MESCA005867"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1791699_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005867-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="17186" checksum="456CA95799CDD240" modified="2013-10-16" version="1">LTPKIQRIFEDENHKLDLYSTKTTQNLFTKLKDKIPVMDKTNEIYKIDCKNCEGKFVSRLINDYLVKIDTKKHHIDNKNTVQYFKFPFIKGLTPKIQRILEDENHKLALYSIKTTQNLFTKLKDKIPVMDKTNLIYKIDCKDCEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQT4</accession>
    <name>T1GQT4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat THOC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02104176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQT4"/>
    <dbReference type="STRING" id="36166.T1GQT4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005999-RA">
        <property type="protein sequence ID" value="MESCA005999-PA"/>
        <property type="gene ID" value="MESCA005999"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060667_0_0_1"/>
    <dbReference type="OMA" id="FTEDWLL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000347">
        <property type="term" value="C:THO complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000346">
        <property type="term" value="C:transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042626">
        <property type="entry name" value="THOC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44411">
        <property type="entry name" value="THO COMPLEX SUBUNIT 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44411:SF1">
        <property type="entry name" value="THO COMPLEX SUBUNIT 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="135"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005999-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="310" mass="35050" checksum="6330D4FF67DA3CD9" modified="2013-10-16" version="1">MKESKLRKLYNNVVSQTISSSGKYLFAGNVFSDIFVFEEPQKHPNIYPQHSGVISCLEFHNDFLIVGAKGSIYGLKFDELKNHLSKDKSWEVSLAEVKNVNCFWLDKAINHIFVGCDNNIIYQVSLEDGRVLRKFEGHKDFVHCLTGSDDQIYSASQDGTLKIWDPRENLHVNEIEPNKKDELKRPELGGFLGACAISNDWLICGGGPKLSLWHTKTLDFSQIDFAFPGKIHCASFIDDNIVVGGVNSSVQIYSINGSLKYDIPIENSPATYSIVFQSDPYKFMSLTGYSNKLNILSDFKYVDIVLDFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRH7</accession>
    <name>T1GRH7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRH7"/>
    <dbReference type="STRING" id="36166.T1GRH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006262-RA">
        <property type="protein sequence ID" value="MESCA006262-PA"/>
        <property type="gene ID" value="MESCA006262"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010025_1_0_1"/>
    <dbReference type="OMA" id="QMNERNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF78">
        <property type="entry name" value="EEF1A LYSINE AND N-TERMINAL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="5">
        <location>
            <begin position="9"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006262-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="379" mass="43202" checksum="B6AD8B9A91244298" modified="2013-10-16" version="1">MDMYDCGFQNMTNIDISEVVIKQMKELNKNKRPVMKFLQMDATSMSFLNEDFTVVLDKGTLDALMTDENQETISTVTKYCNEICRVLKTGGRYVCISLLQEHILKFLLAYFPAHGCMFRVIRCFDAEQKTLESSSDDVMAMPVFVVVITKFKALPTPILETCLGGDKIQRKAALVCNGLAKGNLLDCNEVKMDLLKKTSEGESPRYTVYILDQPYSRGNGKYAVFIVPQGREIEWMFSTPEGRKKVLSSAKHNRLAIVVMHRDQKYESLDDVKNELNDSVRNFAPRGLKDLIPYLTIGSDVGHRETLISGFSNLSGEFRIEEVEGNNGQLFRRLIFLNNQFVIQSEALIQTSKYAFLFQKLRGYRSPNPIRFLFGSTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRL5</accession>
    <name>T1GRL5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006301-RA">
        <property type="protein sequence ID" value="MESCA006301-PA"/>
        <property type="gene ID" value="MESCA006301"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760715_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006301-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7857" checksum="383381C0FB609919" modified="2013-10-16" version="1">MTPCDVGLQAIDEELLSSRKETSHRKLGQSVTIRSHQFQAVHKTSFTWVPRETVLGCKANSSFSQPELNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRU4</accession>
    <name>T1GRU4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRU4"/>
    <dbReference type="STRING" id="36166.T1GRU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006387-RA">
        <property type="protein sequence ID" value="MESCA006387-PA"/>
        <property type="gene ID" value="MESCA006387"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471658_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.200">
        <property type="entry name" value="Translin, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016069">
        <property type="entry name" value="Translin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006387-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9896" checksum="CB923CE8A0AF650C" modified="2013-10-16" version="1">MSSWDDLQKELTYESEGGQKTYCLVDPMEFILGVGDLTGELMRKCINSLGSGDVQTSFAIGNSLKAFFTYYKGLNVHHRELEKPNQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS43</accession>
    <name>T1GS43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine--tRNA ligase, cytoplasmic</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132809">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS43"/>
    <dbReference type="STRING" id="36166.T1GS43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006491-RA">
        <property type="protein sequence ID" value="MESCA006491-PA"/>
        <property type="gene ID" value="MESCA006491"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_3_0_1"/>
    <dbReference type="OMA" id="RNIAREH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006491-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="28811" checksum="60BA8DE9C1F623B5" modified="2013-10-16" version="1">EFFLNVKDLTRHILTEKPSKHFDEWRKQESELQEKYNSSRKQVHEALCDNVDTRTALENLRDLVSTSNIYIRDNKDSLNALLLRKIAQYITDMLHIFGVISGPRGGIGFPVGGNEDSTDILKLCDEIRDEILPNLGVRLEDKDGGAFAVKLVDKDTLLKEKEAKKRAEQEKAAEKEKKKAAAAALAAAKEAQKKIDPKKMFLTETDKYSAFDENGLPTLDKDGKEVSKGQLKKLQKLQQQQETKYKEYLASVTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSU1</accession>
    <name>T1GSU1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02142390">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSU1"/>
    <dbReference type="STRING" id="36166.T1GSU1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006753-RA">
        <property type="protein sequence ID" value="MESCA006753-PA"/>
        <property type="gene ID" value="MESCA006753"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="OMA" id="WPIASCK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006753-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="45559" checksum="14C0877ACF128692" modified="2013-10-16" version="1">MTRKPQILIIDPKLSREILVENFKHFSNNVSSKWSTAKDDVLRMCSPFVCTGNDWKSRRTEIVPALSSTRMKGFHPKTKRICQKLENFIDSKLARDEVSMDVKALAYNFTAEFVADFIWGIDAGAFLESAPCEILRMKDAIIQQAFRCVRAYFLSDLLPFFGNRRFFSKISDKFFVSLLRNLLAKDATFGRSDFINHLEKLKVAKDLNESELTGHTTTVVVDGIETAGAVIAHCLLLLARNENSQEKLRQEINDTLDETLRIFPPLPFLNKICTEPFELENNKGKIVKVSEGDEILISAYAFHHDEEYYKDPEEFIPERFDGDGIKDFRVAGVLIPFGDGPRVCLGNKFGIYEAKVAVVEVIRKFKISVDTTKTRKDNICDPGGFLLSLDSDIWLKFNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSX5</accession>
    <name>T1GSX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSX5"/>
    <dbReference type="STRING" id="36166.T1GSX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006789-RA">
        <property type="protein sequence ID" value="MESCA006789-PA"/>
        <property type="gene ID" value="MESCA006789"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047975_0_0_1"/>
    <dbReference type="OMA" id="NSHECPS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF5">
        <property type="entry name" value="ARFAPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="2">
        <location>
            <begin position="112"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="257" mass="29292" checksum="7982C5A9FD5C1050" modified="2013-10-16" version="1">MTSIKGERSIHEMLNSHECPSMNESQSSILQETDLSGKQVRSQAVRTNLSLSAPCTPISAASPSIPQNGNNDFLRVGALKIDSLKNWSISTYKCTKQSILEKLGKSQRTVDSDLEAQIEQLRETQRKYLSILRLIRAFNSHFHHAVVTQHALAEAFSELAQKSPELQEEIEFDAYRMDLETMSPSQNLDETQQNFTRYKENYEKLRDDVTVKMQFLDENRIKVMHKQLILLHNAIAAYFSGNATALDNTLKQFNIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT61</accession>
    <name>T1GT61_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006882-RA">
        <property type="protein sequence ID" value="MESCA006882-PA"/>
        <property type="gene ID" value="MESCA006882"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834050_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006882-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7753" checksum="46A5BFADDAAD8A6A" modified="2013-10-16" version="1">MSDYILYSPSIQTSPKMMRITQNKKVATHNVNADFYCYAMVTDHEETDIESDEKLWTLYLSKSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT64</accession>
    <name>T1GT64_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT64"/>
    <dbReference type="STRING" id="36166.T1GT64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006885-RA">
        <property type="protein sequence ID" value="MESCA006885-PA"/>
        <property type="gene ID" value="MESCA006885"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_757755_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051115">
        <property type="entry name" value="LAPTM_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479:SF10">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="7">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006885-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="366" mass="41151" checksum="312350043B6DB866" modified="2013-10-16" version="1">MVKKKRQSDLHEGSSTESSEEMKTSSLAAENLCHHTKKAVDTNRLRKVLKPIGLQKTCSECDKNTTQTNGVGGEHGPDPSPDDEEKKLMYALYGIVEHSGGMYGGHYTAYVKVRPKLSPTDKRWKFLPQGSKAELDQEDEQKQKLEELCARQKARSLRNSMCQDAENDSDDLTTSSSSEPEEIDETEGAVGGQNFNDPENIEPPPGKWYYVSDSRVQEVPESTTSFEIQIKMSLKKCCCFDLKTGTIIIGGLDLVFAILSGLKSLFVKGNNNEYSSPIFELFVVTLCISAAILLLVGVKLERPRYFLFYIILKVIEIVLIGIIIILLLITIVALDEEDIKNNMNMRQYENLRLIFVFTEIFLIAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTE3</accession>
    <name>T1GTE3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dehydrogenase E1 component domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02192080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTE3"/>
    <dbReference type="STRING" id="36166.T1GTE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006973-RA">
        <property type="protein sequence ID" value="MESCA006973-PA"/>
        <property type="gene ID" value="MESCA006973"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827888_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="5">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="5">
        <location>
            <begin position="191"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006973-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="336" mass="37404" checksum="1A9A8B168F93A963" modified="2013-10-16" version="1">MGTSAERAASNTDYYTRGDALPGIWVDGMDVLAVKAATSFARDYVTKNGPIVMETFTYRYSGHSMSDPGTSYRTREEIQEVRQKRDPITSFKEKILELGLLTQEEVKAIDVKVKKEIDDAAAKAKADVEIPLEELYTDIYSTCLEPKLRDCVGYNQAHRVLNKGPYKMYNIDECPSHSAKISKEMGIKYLRDMMLIRRMENECSNLYKSKLIRGFLHLCVGQEAVNVGLHAAMRPEDHLITAYRSELAGREAGCSRGKGGSMHMYAPKFYGGNGIVGAQVPLGAGVALHEKILNKKGVCFALYGDGAANQGQIFEAYNIAHLMKLPVIFVCENNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTZ2</accession>
    <name>T1GTZ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTZ2"/>
    <dbReference type="STRING" id="36166.T1GTZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007191-RA">
        <property type="protein sequence ID" value="MESCA007191-PA"/>
        <property type="gene ID" value="MESCA007191"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507098_0_0_1"/>
    <dbReference type="OMA" id="RIDSKCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007191-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="20214" checksum="CCFD39521E2D17DF" modified="2013-10-16" version="1">VPICRAIAPKSTSGIATNVLCHPTLSNFFFVCGERHLRVWSILREQRKLNVHDVNMGKLQRKFTGMRIDSKCEYLYLGTMTGDVIKVRLNCCDPENVTKKGMNSLIIGAFAKHNPRKPKGKDCVQYVCGVRDLYVLNECTFLIGAGDGTVDMVVERDVCLKDYPNPTWPLYRATKTVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU01</accession>
    <name>T1GU01_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU01"/>
    <dbReference type="STRING" id="36166.T1GU01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007200-RA">
        <property type="protein sequence ID" value="MESCA007200-PA"/>
        <property type="gene ID" value="MESCA007200"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1231143_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007200-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="225" mass="25771" checksum="1424DDDD238BD36A" modified="2013-10-16" version="1">MSSVEGPCTIINSGDIDPYNGMQLKQDLEKGKLFGFLVDNKKDKRPPLRQYFMDGDFFIGASLCVTLTKLALRYIELEKNEVEQNRFCARMMLVMSSILHLGKSGFPTKPITNDDTDRIFLCLKTLSERTPETVEVFQHFCRDALGKMLEAQHDEEAQALKEKQKSASKVQADDPVQFAQLSSGKESQLGENVFESSLMKEEKEIFLNRRIFVKTRDSQVDFFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU14</accession>
    <name>T1GU14_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007214-RA">
        <property type="protein sequence ID" value="MESCA007214-PA"/>
        <property type="gene ID" value="MESCA007214"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064124_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007214-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13492" checksum="D678A36CAF597E5E" modified="2013-10-16" version="1">MCRRAIQCYKCSSYSDLNCAKSELLKSSSMANVVNCDFDRRPNYILSSAPVTKCAYMTIKDAAGVVVKRDATMKTTHKDLICAMLDQMWTLANARFAREISATLEPEWLLESLHWPPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU66</accession>
    <name>T1GU66_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007274-RA">
        <property type="protein sequence ID" value="MESCA007274-PA"/>
        <property type="gene ID" value="MESCA007274"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742961_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007274-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7881" checksum="1C2D9A111356EDD3" modified="2013-10-16" version="1">MTNSKFFFRIGIDIVATLIPTYQVASSKLQVAALFTKKLLDGYLDGGTMNAIKLSLRNIGFMNSIFIAYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUF6</accession>
    <name>T1GUF6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188040">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007368-RA">
        <property type="protein sequence ID" value="MESCA007368-PA"/>
        <property type="gene ID" value="MESCA007368"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500498_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007368-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="10070" checksum="418F84ED6F5B2419" modified="2013-10-16" version="1">MIPRYLSSAKVNFTPHVLENVINNEERATVESRDRMVFTYHRAHGRFEFLSCRHVISMYFTKKGNYPQPQKKKCVAGVSSRPVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUJ7</accession>
    <name>T1GUJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUJ7"/>
    <dbReference type="STRING" id="36166.T1GUJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007411-RA">
        <property type="protein sequence ID" value="MESCA007411-PA"/>
        <property type="gene ID" value="MESCA007411"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_854111_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="139"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007411-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="326" mass="37527" checksum="774E8A276E7F283F" modified="2013-10-16" version="1">FQEKHVTRTELVQYIQKLLKSDSNVQTDYTSTQYALTTSLSSISIKNTSTSSNSFINLPNVKVVFLMHEKLTANMRRRYENQVTQSMSSTLIQFAKKESFWVLVVDLPPSELNALVGAINPREVTKKETESEINVVIESLPKISLEESKGISDTLLEGLLQNLNEQAASLKGEVMIYDLTQTVQSFLHAHNKPPPGSLYDQMLEERKKRQEELLMEKMQKENEHRQALQEEIQRRKELFQKENRRREPRRSMSESNRQASSSESSENSSPYFRGHVVSKCLDHRSTETLYFPKIGRQIRRGCCVGHSQKGCIAYSGLIWILVNFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUP7</accession>
    <name>T1GUP7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GUP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007465-RA">
        <property type="protein sequence ID" value="MESCA007465-PA"/>
        <property type="gene ID" value="MESCA007465"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237704_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cadherin" evidence="2">
        <location>
            <begin position="30"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007465-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="25166" checksum="DA601865E25BA6FE" modified="2013-10-16" version="1">MGKGCSATEAQHISQINTPPVMYVDQHWRITEKEENGSIIVRVRAEDAENDILQFGLEPHYLNRGKNIDSYPFRIDSSTGVVYLNESLKGRGGESFFLYITVTDGQLTAKNEVLVDILKSDDNSKLYEIRNPSFTQTVRNLSQVLPPFHLLPGVINSRPLNNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV67</accession>
    <name>T1GV67_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007651-RA">
        <property type="protein sequence ID" value="MESCA007651-PA"/>
        <property type="gene ID" value="MESCA007651"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125656_0_0_1"/>
    <dbReference type="OMA" id="KICANDH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577751" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007651-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18186" checksum="BC887DB85355000E" modified="2013-10-16" version="1">MTKIIFSVLFCCFLAESLACNKFKLNLRSVQNCAGDDAIVKVENGLRVTLTTECTIKVSGCADFKGFSTATAHYVIKKGILTVKRGSEDVCARLAELPADLKAQGAPDKCPVAANRVCVSDYTIDISQYKQYLPLAKGRSFLDINVDHDTGKSCFRVEADVTKSWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB4</accession>
    <name>T1GVB4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007705-RA">
        <property type="protein sequence ID" value="MESCA007705-PA"/>
        <property type="gene ID" value="MESCA007705"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186935_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007705-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12458" checksum="64B1BF1EEA90021A" modified="2013-10-16" version="1">MNQFGIPKLIKLYQITLRNTWSSRFSTRRRTIMIKKQKSGQFLTYANDIDVIGRTPSEVIDKFLAIEKLANSVGLNVSGSKTKFVISSNITFGHRTDKIKNKYNKSVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVQ7</accession>
    <name>T1GVQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVQ7"/>
    <dbReference type="STRING" id="36166.T1GVQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007873-RA">
        <property type="protein sequence ID" value="MESCA007873-PA"/>
        <property type="gene ID" value="MESCA007873"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1074758_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039919">
        <property type="entry name" value="ARHGEF10/ARHGEF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877:SF7">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="190"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007873-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="259" mass="28940" checksum="8090B8AEE7BECB1B" modified="2013-10-16" version="1">MNESLGDVFLKAFSQPQVLEIYSGFINNFSAAMELAKMEAKRKSALADFFKVKQISAHDRLSFFGLMVKPVQRFPQFILFLQDLLKNTPQGHHDRTSLQLALTQLESLAEMLNERKREAEQYQAFKEMLGHISGTFNTRSFSSASHKGASDLGNISEMMSAEIHEEKLTKDEETQQMAPAAATLKRQKNKANAAANSKNSPNSEGPTTVITLMGGRGYWKHVWYNSSGTSPSTGKNNSSSNSRFVNSNDAHIVIWEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW54</accession>
    <name>T1GW54_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW54"/>
    <dbReference type="STRING" id="36166.T1GW54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008025-RA">
        <property type="protein sequence ID" value="MESCA008025-PA"/>
        <property type="gene ID" value="MESCA008025"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631254_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008025-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18098" checksum="D498913BAB28C495" modified="2013-10-16" version="1">MSIIGTEICSPQHKSKQNHKKKLANHHIIGVLGASATNTPTSTSTPPMDVTPPKSPSFLMDTSAKNLNTYHEMMSNSSEVGKNLLETEDDETINMPIYNSHVFKNNNVDDDDDEEDDQEQEEQKKEEHMNAAIDLSAQSSTPIKDDEETKVSMEEESSTNTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWM8</accession>
    <name>T1GWM8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008206-RA">
        <property type="protein sequence ID" value="MESCA008206-PA"/>
        <property type="gene ID" value="MESCA008206"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1612704_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008206-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="18034" checksum="86817F377457DE71" modified="2013-10-16" version="1">MIRGFPQGEELHCSFFDILFIVSWRKALGSGFGDSSFSIFIAMGSENLNTIFSLNGLVFGNENCCESDFILEDIEFGRNFEHISLRGLKFQQVASTKSSTIPATKSNSIAATNLKPTPFAQHVASKVTDLSTKDIVSSASISNLKPTSFTKHDASTKLLTTLQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWS0</accession>
    <name>T1GWS0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin-related protein 2/3 complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARPC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWS0"/>
    <dbReference type="STRING" id="36166.T1GWS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008250-RA">
        <property type="protein sequence ID" value="MESCA008250-PA"/>
        <property type="gene ID" value="MESCA008250"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187809_0_0_1"/>
    <dbReference type="OMA" id="XETILAR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008384">
        <property type="entry name" value="ARPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629">
        <property type="entry name" value="ARP2/3 COMPLEX 20 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05856">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008250-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="9142" checksum="818D0BA29D1CBB76" modified="2013-10-16" version="1">MMRRAESFIILRRKPIEGYDISFLITNFHTEQMYKHKLVDFVIHFMEEIDKEISEMKLAVNARARICSEEFLKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXF8</accession>
    <name>T1GXF8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02031219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008508-RA">
        <property type="protein sequence ID" value="MESCA008508-PA"/>
        <property type="gene ID" value="MESCA008508"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515243_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008508-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="10025" checksum="7150CED4BF77D9E1" modified="2013-10-16" version="1">MACVIECITYIWFLPISSGETHVGRWIWGEGSIYCLIDKDTLLREVEKYVDKVFDERFLYPDRMPTVQLLGKCCGKEGYEDYQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ6</accession>
    <name>T1GXJ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008548-RA">
        <property type="protein sequence ID" value="MESCA008548-PA"/>
        <property type="gene ID" value="MESCA008548"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3351623_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008548-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="37" mass="4219" checksum="6AE85168FA233A77" modified="2013-10-16" version="1">MLQVPTSTILPNFIHIQKSYGKTVECNKPSKDQMNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXP2</accession>
    <name>T1GXP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dehydrogenase/reductase SDR family member 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185752">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXP2"/>
    <dbReference type="STRING" id="36166.T1GXP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008595-RA">
        <property type="protein sequence ID" value="MESCA008595-PA"/>
        <property type="gene ID" value="MESCA008595"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_19_1"/>
    <dbReference type="OMA" id="ERNIAGH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008595-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18950" checksum="4CC7B26057610E7F" modified="2013-10-16" version="1">MLKPEDISNAIVYCISTPPNNEMDRFRNKIAVVTGSSSGIGQACAIALVKSGMIIKALARRENKLEEIKDSLPADFRDNFHPIKCDVSKEDEVIKVFQDINEQHGGVHVLVNNAGIYRPEHQLTFRNNTNDILDTINTNIMGTMYCTREAFHSMKERNIAGHIINMKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY26</accession>
    <name>T1GY26_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Cytochrome c oxidase subunit 5A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Cytochrome c oxidase polypeptide Va</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02002342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY26"/>
    <dbReference type="STRING" id="36166.T1GY26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008746-RA">
        <property type="protein sequence ID" value="MESCA008746-PA"/>
        <property type="gene ID" value="MESCA008746"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707631_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008746-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8269" checksum="865B6D0E6D17EF0F" modified="2013-10-16" version="1">MLRTGAVRASHGSSETDEQFDQRYVDYFNRKDIDGWEIRKGMNDLLGMDLIPDPKVIEAGLKACVNEISLILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYL6</accession>
    <name>T1GYL6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYL6"/>
    <dbReference type="STRING" id="36166.T1GYL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008949-RA">
        <property type="protein sequence ID" value="MESCA008949-PA"/>
        <property type="gene ID" value="MESCA008949"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577831" description="Sushi domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="4">
        <location>
            <begin position="45"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008949-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22647" checksum="6087B7E959EEFBA9" modified="2013-10-16" version="1">MKIYFILFLFQVLSQGLLIFAYDDTSQDFLETLDASCIASRVPAPDINNGYVIKYDRKSFKDISYVIALYACDDNFEFEGNTSNVVYCSKDNWIGNLPKCKGTEDYNDYEEDDEDDEPSKNENYDSSESENNLVYQNEKNDLYENSEQERQTVIQSEGDDISNVINIEVAEEASVFEKKPIECPSNHECEHNCISAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYQ6</accession>
    <name>T1GYQ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYQ6"/>
    <dbReference type="STRING" id="36166.T1GYQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008993-RA">
        <property type="protein sequence ID" value="MESCA008993-PA"/>
        <property type="gene ID" value="MESCA008993"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_2_2_1"/>
    <dbReference type="OMA" id="DLYNFPM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008993-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16086" checksum="16D5DE78B6FE29EF" modified="2013-10-16" version="1">MTYLAEKYGKIDSLYPKDPAKRAVVNQLMYFDMGTLYQRFADYYYPQIFGSGSADDEKSKKMQEAVEFFNKFLEGRTYAAGNNLTLADLTLLATISTYEVAGFALKKYPNVKKWYDKVRKEAPGADINQKGADEFKQFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZM3</accession>
    <name>T1GZM3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02118258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009327-RA">
        <property type="protein sequence ID" value="MESCA009327-PA"/>
        <property type="gene ID" value="MESCA009327"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815370_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009327-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7707" checksum="A6F0DC144A81832F" modified="2013-10-16" version="1">MLVLFWGLTLFIVSVIGVFNTQLENNGLMAFYLTFLLLTQLIFTFYLSLNRNEAFTTLKKLLGVIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Q5</accession>
    <name>T1H0Q5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calnexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the calreticulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02065477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Q5"/>
    <dbReference type="STRING" id="36166.T1H0Q5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009740-RA">
        <property type="protein sequence ID" value="MESCA009740-PA"/>
        <property type="gene ID" value="MESCA009740"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171326_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009740-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12987" checksum="B311EF1B7D612C38" modified="2013-10-16" version="1">MDGEWEAPLIDNPICEKASGCGEWKAPQIDNPNYQGKWRAPLIDNPNYQGKWSPRKIPNPDFFEDLNPFHMTPISAVGIEIWSMSDDILFDNLIITDDVEFKEIADYFVVRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H122</accession>
    <name>T1H122_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02028440">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA009873-RA">
        <property type="protein sequence ID" value="MESCA009873-PA"/>
        <property type="gene ID" value="MESCA009873"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375276_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009873-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="22097" checksum="95364F54E963B964" modified="2013-10-16" version="1">MGVFSEHLPAFSFSSGAMEACLAVKNKNQFGTALRRHVRNAAKRLKVSQPHPISKDNLVNNIDADIAIVEKYIPNIDLCETRDELKGELSWFKYSKSVFNMAKNKKDEKVDAFVSLISDDEDDEMSDNDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTVQNSITTISSTPEPMEVQSAPPPTPIETPTSTVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H132</accession>
    <name>T1H132_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H132"/>
    <dbReference type="STRING" id="36166.T1H132"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009883-RA">
        <property type="protein sequence ID" value="MESCA009883-PA"/>
        <property type="gene ID" value="MESCA009883"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021286_0_0_1"/>
    <dbReference type="OMA" id="SWINIGY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF24">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="3">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009883-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="14559" checksum="5133C1F7719DD568" modified="2013-10-16" version="1">MECVGRLHTIKLAMIPCIAGWIAIAMADNFYILLIGRILTGLGCAIGTSPAIVYITEVARPDLRGSLMSTAPTIASFGMIIAYAEGAFMHWRLVSWINIGYTIIPCILVNSLYQNHLFGWFLRDASRMLGNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H158</accession>
    <name>T1H158_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H158"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009911-RA">
        <property type="protein sequence ID" value="MESCA009911-PA"/>
        <property type="gene ID" value="MESCA009911"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3414391_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009911-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="28" mass="2999" checksum="0C879CC7987FEB1F" modified="2013-10-16" version="1">MIACLGETTGDLSLSKNLELMKSTEEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H175</accession>
    <name>T1H175_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H175"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009929-RA">
        <property type="protein sequence ID" value="MESCA009929-PA"/>
        <property type="gene ID" value="MESCA009929"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725071_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009929-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8481" checksum="FAD5A261E12DBD50" modified="2013-10-16" version="1">MLITYGGNVLKWCKYLPLRLVPPNYVKSNDYHLALLQGGNRCYHSWRILAYYRAHMVVVAVRGPVRYKQKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1B8</accession>
    <name>T1H1B8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1B8"/>
    <dbReference type="STRING" id="36166.T1H1B8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009977-RA">
        <property type="protein sequence ID" value="MESCA009977-PA"/>
        <property type="gene ID" value="MESCA009977"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_835628_0_0_1"/>
    <dbReference type="OMA" id="RAICINE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="1">
        <location>
            <begin position="124"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009977-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="333" mass="37646" checksum="715829690254C59B" modified="2013-10-16" version="1">MHAKENCDGKTRMHNVRFYGLSPRGIKCMALNEETKKLALSRNDASIEIWDMNFAPFLERVIPGEKNSSIESLVWAQTQNRLFSAGLTGELVEWDLQTLQKRNRQLATGSAIWCMAIDKDETQLAIGTEEGYINVFDITNEELVYKTLFDKQDGRILCCKFNNNGNFLVTGSIDVIRIWNCKTGHALKKMTMSRYEAKKETVVWSLLVLRDFTIISGDSRGVVTIWDGNNGTYIESHQALKSDILAVAMNSTEDMIMVTGVDPVIRIYAETVIKKQDTEVSSWVKYLQRSVHDHDVNSLIVNNDNIISGGVDGYIAEWIANLFLIHQCVLVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1J6</accession>
    <name>T1H1J6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1J6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010059-RA">
        <property type="protein sequence ID" value="MESCA010059-PA"/>
        <property type="gene ID" value="MESCA010059"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712875_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010059-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="388" mass="42718" checksum="F73493754336A36D" modified="2013-10-16" version="1">TEAIEQSDIKSENEEQVNEAENKDNNANNEPISSDNSESISNPEKAVSNIEAPKETKKEENGLQTKNYEDDSVIVTDQSFSSTNTGTIEQSLIKPENDEQSDSNVSEDAETVGKGLKIMKEGDKIIESEIKTENTENQGNTDESKIMSNPVEQSEVNTFEKKYSNDNSESISNPVETVKMSENCTTGLEVDSNKDIEAVSADLGSNLENPESMINMDKVPSDCDTSGKLETENTSQKEDEPEIMESRTTNSESITKIEHSPDKNIPKADINKIEIDSIQDNIEVSADLRINPDPETNPENPESKENVDHVILESDVIGKAETENTSLKSVEAEVTESKASDSESITNLENSSDTNVTNDNTITGSLQEVKKDEIDNVPEIKEVRDKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H202</accession>
    <name>T1H202_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02062397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H202"/>
    <dbReference type="STRING" id="36166.T1H202"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010231-RA">
        <property type="protein sequence ID" value="MESCA010231-PA"/>
        <property type="gene ID" value="MESCA010231"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2489742_0_0_1"/>
    <dbReference type="OMA" id="WAYWHIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001735">
        <property type="entry name" value="Opsin_RH1/RH2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00576">
        <property type="entry name" value="OPSINRH1RH2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010231-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10235" checksum="D8825858329AD982" modified="2013-10-16" version="1">MYTIFVYYIPLFLICWAYWHILSTVSAHEKAMREQAKKMNVKSLRSQEDCDKSAEAKLAKVALVTISMWFCAWTPYMIINTLGLFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H227</accession>
    <name>T1H227_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02076197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02076198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02076199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H227"/>
    <dbReference type="STRING" id="36166.T1H227"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010258-RA">
        <property type="protein sequence ID" value="MESCA010258-PA"/>
        <property type="gene ID" value="MESCA010258"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533969_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010258-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9273" checksum="24A1D0E8D1114B35" modified="2013-10-16" version="1">MLSTSNFVLPMTSMSSAYRKSSSLGGTSTALTGILLCLAKNQDKQELLRQEVSQIKNKSLTANDMNNLPYLRACIKEGIRMYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2M4</accession>
    <name>T1H2M4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 216</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02123485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2M4"/>
    <dbReference type="STRING" id="36166.T1H2M4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010474-RA">
        <property type="protein sequence ID" value="MESCA010474-PA"/>
        <property type="gene ID" value="MESCA010474"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135948_1_0_1"/>
    <dbReference type="OMA" id="LRIEYIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019184">
        <property type="entry name" value="Uncharacterised_TM-17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531:SF0">
        <property type="entry name" value="GEO07735P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531">
        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09799">
        <property type="entry name" value="Transmemb_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010474-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14256" checksum="7337FF9360338AED" modified="2013-10-16" version="1">MNSSLFYEIFMYLNSFYFGMFAFSELSIVNESCFLFGICVLEISRIILGRKGSLSEYSWKVYLSVVLTIPSLCGVLYFLLYQTYILKLEYIICALLVALHAAEIMFALVFFVTLCRPSEYYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2V9</accession>
    <name>T1H2V9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101248">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2V9"/>
    <dbReference type="STRING" id="36166.T1H2V9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010565-RA">
        <property type="protein sequence ID" value="MESCA010565-PA"/>
        <property type="gene ID" value="MESCA010565"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2405688_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="3">
        <location>
            <begin position="9"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010565-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10370" checksum="ABCEB82A4B6FF8C5" modified="2013-10-16" version="1">MNHQNFTDSPKPFPPWKGIRSAKKDGSKCTKCYGSKPDDPTEGSEDCLFLNVYTPPLKRISKHGLPVIVWIHGGSWLGGSNDHKIYGPDFLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3G1</accession>
    <name>T1H3G1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3G1"/>
    <dbReference type="STRING" id="36166.T1H3G1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010788-RA">
        <property type="protein sequence ID" value="MESCA010788-PA"/>
        <property type="gene ID" value="MESCA010788"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1171793_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010788-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26310" checksum="777BD5E25D77FFC4" modified="2013-10-16" version="1">MGSVWFEKNLNTFFVHILELVANPKAASSHVDAVYSRKCINFILRSVTGKMLGEKAQTSACKELILIIAKQMKSIDFTPENAKDSNQETLFSQHLLVCALQELGCLTLGLGTTTQNLINDTEELLRSASQNSRMSLHRTQAGWLLIGAIMTLGSPVVKNLLPRLLLLWRNSFPKTTKDLESEKARGDSFTWQVTLEGRAGALSVMHSFLQNCNDLVTDDIIKKIFSPIESALAMLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Q4</accession>
    <name>T1H3Q4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Q4"/>
    <dbReference type="STRING" id="36166.T1H3Q4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010888-RA">
        <property type="protein sequence ID" value="MESCA010888-PA"/>
        <property type="gene ID" value="MESCA010888"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067237_1_1_1"/>
    <dbReference type="OMA" id="AGWYCEA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002461">
        <property type="entry name" value="Zinc finger matrin-type protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="7">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010888-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="22045" checksum="47F8E79E8798ABA3" modified="2013-10-16" version="1">MRREVELNSPRTSDVTNEKQFASTSEVAADVERPNLSKEENLKTRDFQVDFDAKLGKSVVINKNTPGSSQGYYCSICDCVIKDSINFLDHINGKKHQRNLGMNMKVERSTVDQVKERFKLNKIKMETKQKDYELETRIKEVKEEEERYKEHRKEQRKDRKRKIEETEDDGNDELRAIMGFSGFSSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Y0</accession>
    <name>T1H3Y0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Y0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010977-RA">
        <property type="protein sequence ID" value="MESCA010977-PA"/>
        <property type="gene ID" value="MESCA010977"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433188_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010977-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10209" checksum="D53015394CB7244D" modified="2013-10-16" version="1">MFHEIIAMIIDVIKIIASIVACGVIGSSENGRTYSKYSNYEYYSYESNSNNGLVGALAVFAVFNIGFFALRTYIFIGIWSLFKNIRRGNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H464</accession>
    <name>T1H464_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H464"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011066-RA">
        <property type="protein sequence ID" value="MESCA011066-PA"/>
        <property type="gene ID" value="MESCA011066"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2090797_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011066-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13137" checksum="9931A54A4C34C478" modified="2013-10-16" version="1">MSSDNDFLQGNSVKLNKVECGGSERYIQNFICSTKLFEKETILKIELDLMFILNNFTAKLQLFKQQLGGFKPFVLNIKAEGCGILSGKSKSPHPILTIIRKVIMENLNISKSCPIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H487</accession>
    <name>T1H487_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4794 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H487"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011091-RA">
        <property type="protein sequence ID" value="MESCA011091-PA"/>
        <property type="gene ID" value="MESCA011091"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_597567_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577871" description="DUF4794 domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="50026" checksum="E36BCA113EC0C3C6" modified="2013-10-16" version="1">MKCVLSVLVLVAVGALAIPHGGYMYYKPATQGIMYVQYQKNGVGSQSDGVSAFVAGESGNTKRLSMMYLLVEVQTGEDVLSDEIAAKVEGQQTISVAQGYNSESIAEAYPAEESVNDYDGQAKVEVDAEVPKEYLPPNNEYLPPVNQEKAIFNEISNQESVSLTKTEDVVEEAVADDVAVVQEDVIPVSVVESEVNEQVKKVEVSDAVTEIKSVQTEYGVPKVEDSDREPKNIAEETPAVEVSEVKNVEEKVETEETVKTVSEDEESVKVAEVPVENVPAVVESEDVPLADDDSTLVVEEPTPVVKDIPQPEPVPFVPVAPKPTKRTRLYPAKRPSFVPQRKKVYVQLEQENEEALEYVPAVHNRQVAKPKKEVVAVDDEEEQQSPLPLGELSSQLALVEPAEELLPLPTVSAPAKEVHQRVKLLPTEVPVIHSEPKTHPNTLKPIAIVRKIEESGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H490</accession>
    <name>T1H490_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H490"/>
    <dbReference type="STRING" id="36166.T1H490"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011094-RA">
        <property type="protein sequence ID" value="MESCA011094-PA"/>
        <property type="gene ID" value="MESCA011094"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_684398_0_0_1"/>
    <dbReference type="OMA" id="RNINDGW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035195">
        <property type="term" value="P:miRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052068">
        <property type="entry name" value="GW182_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13020:SF25">
        <property type="entry name" value="PROTEIN GAWKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13020">
        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="192"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="364"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011094-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="403" mass="43482" checksum="4BF744A9CA7D44E6" modified="2013-10-16" version="1">NLSDGWADSTNDNENKDWPTTQASPAPAFTDLVPEFEPGKPWKGSQMKSIEDDPSITPGSVARSPLSINPMPKDAAEIFTTNQTNKNSPTDLPPLSLSSTTWSFNPTNLSNQNFARYIIRQQNATAAELWGAPINKTSRGPPPGLGSGSKNVAGGLNKSSANGWIGSGRGTGNSGAVSWASSGNTSWNTNSNWLLLKNLNNQIDGSTLCTLCKQHGPLLNFQLYLNQGIALCKYTTREEAQKAQMALNNCVLGNTTISVESPNETDVQNILQHFPLVNSGGNNGNNVPTNSGNSGNGNSSRQQQQVRSVGGPGRDDVDPYDIFSNLVCAIIPSKYNFKISRKIKWWSTSRFLEREFLWQTGGTSSSSLWTLETSSSGNGGNQNERTTPSNLKSLLPENLLELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4H6</accession>
    <name>T1H4H6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylate kinase active site lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4H6"/>
    <dbReference type="STRING" id="36166.T1H4H6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011185-RA">
        <property type="protein sequence ID" value="MESCA011185-PA"/>
        <property type="gene ID" value="MESCA011185"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_1_1"/>
    <dbReference type="OMA" id="TIAHFST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="7">
        <location>
            <begin position="126"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011185-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22091" checksum="A6A18E39D55E252B" modified="2013-10-16" version="1">MSSKLFKAIILGAPASGKGTISKRILDKYGFKHIASGDILRMNIANNTELGVAAKKYIDEGKLVPNDLIIKCIMTRIDECNSSSWLLDGFPRTFEQAKQLHSVHKVNAVINLNVPFEVIVDRVKGRWIHLPSGRVYNIGFNEPKVPGKDDETGDELVQRPDDQPDVVRKRLEVYQDTINPVIEFYEKLGLVQGQDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4P7</accession>
    <name>T1H4P7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4P7"/>
    <dbReference type="STRING" id="36166.T1H4P7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011263-RA">
        <property type="protein sequence ID" value="MESCA011263-PA"/>
        <property type="gene ID" value="MESCA011263"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_666624_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2130">
        <property type="entry name" value="DEAH helicase family, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042035">
        <property type="entry name" value="DEAH_win-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF237">
        <property type="entry name" value="ATP-DEPENDENT DNA_RNA HELICASE DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase associated" evidence="5">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011263-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="413" mass="47806" checksum="25C97C68B8705947" modified="2013-10-16" version="1">MSATFSFETFSKYFENCKVFQVQGSLFPVEEKYLEDAIEETQFYKFPEPRYSTKNKRSREDKFSSEFDGLIAPYARNLRGYSRRTTEAIMNPESEKINIELIEKLIFHISETKDQGGILVFLPGYGTISDLLQALTKNPRYNSSRFWILPLHSMMQSSEQKRVFDPAPNNVRKVCTDYDIERNCQTLEEQFTTLSNSRQRRGRAGRVRPGICYHLYTRAREMVLDNFPTPEIQRSRLEGVILNFKVLHLNNVEEIFKEMIDKPNEQTVLNGLTLDHLMVANVVGGGVEEQFTTLSNSRQRRGRAGRVRPGICYHLYTRAREMVLDKFPTPEIQRSRLEGVILNFKVLHLNNVEEIFKEMIDKPNEQTVLNGLTLLKRIEALDENETLTPLGLHLAHLPVDPQMGKMLLLGALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Z9</accession>
    <name>T1H5Z9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5Z9"/>
    <dbReference type="STRING" id="36166.T1H5Z9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012132-RA">
        <property type="protein sequence ID" value="MESCA012132-PA"/>
        <property type="gene ID" value="MESCA012132"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055193_0_1_1"/>
    <dbReference type="OMA" id="VRWPKAV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="2">
        <location>
            <begin position="113"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012132-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="25156" checksum="9E4270A41C7A57B0" modified="2013-10-16" version="1">TKKVAPAALAVKKKVEKVVNRLFEKRPKKIVIGQNIQSLFVRLPKYIQRQRAVLLKPFNQFSRALDKALKFKLLEKYRSESVVSKWKIVEAKGKSQVVQPKKKPIFLCAGTNIAHDADPIEMVLVLFLPALCRKMGVPYCIVKGKARLGLLVRRKTCSAIALTATEAGDRANFSKIVEAVKTNYNDRHDEIRKHWGGGILGSKSMARIAKLEKAKARELAQKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H650</accession>
    <name>T1H650_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit Tim16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H650"/>
    <dbReference type="STRING" id="36166.T1H650"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012183-RA">
        <property type="protein sequence ID" value="MESCA012183-PA"/>
        <property type="gene ID" value="MESCA012183"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101461_3_0_1"/>
    <dbReference type="OMA" id="AKYLIQI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012183-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13825" checksum="E26167A0B52BE148" modified="2013-10-16" version="1">MAKYLAQLIVLGGQIVGKAFAKAIRQEIRMSQEAAKRAGGGRQGDKSAVSNSKTGLTLEEAKEILNVSKLDDPQQIQKNYEHLFQVNDKSKGGSFYLQSKVFRAKERIDDELKNIKIQESKAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6G1</accession>
    <name>T1H6G1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA-directed RNA polymerase I subunit D</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6G1"/>
    <dbReference type="STRING" id="36166.T1H6G1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012294-RA">
        <property type="protein sequence ID" value="MESCA012294-PA"/>
        <property type="gene ID" value="MESCA012294"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_4_2_1"/>
    <dbReference type="OMA" id="ITHATFC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07029">
        <property type="entry name" value="RNAP_I_III_AC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerases I and III subunit RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033898">
        <property type="entry name" value="RNAP_AC19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="8">
        <location>
            <begin position="12"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012294-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10522" checksum="1347D199C2BC9670" modified="2013-10-16" version="1">LAGDENSDEGCRTFVITDEGHTLGNALKCIICRYPEVDFCGYTIPHPVENKMHFRIQSKGRAVDILRRGLEDLEKLCDHTVETFEKEFQSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAJ4</accession>
    <name>T1GAJ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The WWE domain mediates non-covalent poly(ADP-ribose)-binding.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">Ubiquitinated; autoubiquitinated.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02165413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAJ4"/>
    <dbReference type="STRING" id="36166.T1GAJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000251-RA">
        <property type="protein sequence ID" value="MESCA000251-PA"/>
        <property type="gene ID" value="MESCA000251"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1103835_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072572">
        <property type="term" value="F:poly-ADP-D-ribose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.50:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033509">
        <property type="entry name" value="RNF146"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018123">
        <property type="entry name" value="WWE-dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13417">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13417:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00678">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="9 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WWE" evidence="12">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000251-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="28197" checksum="0A0965D8F487BE76" modified="2013-10-16" version="1">MCRREIPPEFLDHPQLVNGIQDICNTKATEDGYQWFYEGRNGWWQYDDRTSSDIEEAYKKEEKSCTILVAGYVYIVDFEGMVQRRQTDPSRVRHVKRDLATIPKKGVAGLRIEGNTVTTDSNFANQIYQDQEEQVFEVENENVGFLSSISAAHAAIRIANDIIGSTLSLEEPVQTNSRSVNGRSESTSSNRSRLSSRNGSSSSQESSSTSDLVSANENEEILLDGLDARGSSDLQISELLICSNKLWIISDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAQ5</accession>
    <name>T1GAQ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Peroxisomal membrane protein PEX16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peroxin-16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAQ5"/>
    <dbReference type="STRING" id="36166.T1GAQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000315-RA">
        <property type="protein sequence ID" value="MESCA000315-PA"/>
        <property type="gene ID" value="MESCA000315"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1483644_0_0_1"/>
    <dbReference type="OMA" id="ETTAKWI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013919">
        <property type="entry name" value="Pex16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08610">
        <property type="entry name" value="Pex16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="20971" checksum="3E00B144AA445403" modified="2013-10-16" version="1">MKSVKDANIQLKELFQNYKKFVSSNPQTISDLETTAKWISYFIAGRISDSSVTSELVYALSNMLVFCNDRIIENSQNPKAEVNPTAQKIKVMLTTLEYTEVFIEIGSRRLWGKKARWYFIVAIQIIKSKVSRDTLYESVAAMLQSSTDKKRNFLETVELQIGLKNYDPQKDKRFSGTVKYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB84</accession>
    <name>T1GB84_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02048203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000506-RA">
        <property type="protein sequence ID" value="MESCA000506-PA"/>
        <property type="gene ID" value="MESCA000506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_947621_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="294" mass="33775" checksum="BF89C7462C8A69A8" modified="2013-10-16" version="1">MLNITSDFDFTPKHHKFSESPDLKLDNPEDLFDVERILEALDTRPDLQLRTPSPQEEIYISSQELRGEAQSQQHNLKTPSDLKFECSEPKTPPRTPVKRKALKVINPGKEFNDEGEVLIIKLKSSFNIPGILLYRDIATEFTVLRVLTKSCSIEDIAKELWKIFSNFGFPSKLQTEGKLYKLYPGNGVTMNHNPIMRPIYNRKTKDQVRLLINQWIVENLSNDLEMACHKVQWMINTSTVNYGPCPLYGVFLGKVGFVHENPEQDSFFRAVGYTSKRLSRNSFVDSDSDRSSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCJ4</accession>
    <name>T1GCJ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCJ4"/>
    <dbReference type="STRING" id="36166.T1GCJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001009-RA">
        <property type="protein sequence ID" value="MESCA001009-PA"/>
        <property type="gene ID" value="MESCA001009"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000526_4_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="13"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001009-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9881" checksum="C62909D028D117DA" modified="2013-10-16" version="1">MEKLMCNDGLIFKPKGQRNEIDIQIGQLVLIIDEDLSPTTWPTAIVTELFPGKDNVTRVATVRTSSGKTYKRPVVKLRILPCNTPLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCJ9</accession>
    <name>T1GCJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Snake toxin/toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001014-RA">
        <property type="protein sequence ID" value="MESCA001014-PA"/>
        <property type="gene ID" value="MESCA001014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126188_0_0_1"/>
    <dbReference type="OMA" id="ITPCINY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577267" description="Snake toxin/toxin-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13525" checksum="E37E560E894E3E77" modified="2013-10-16" version="1">MLRTPVYFFIIISINIQFINGLKCHMCGQYNEGGGSITPCLNYSESHANSFLKECSKPTEKFCVKYVSELSPVRDCATECTEKEVWQSKTFCCTEDGCNTANILQLSILNAFLMLFIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCZ2</accession>
    <name>T1GCZ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001166-RA">
        <property type="protein sequence ID" value="MESCA001166-PA"/>
        <property type="gene ID" value="MESCA001166"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909071_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001166-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15194" checksum="DDDC0CC1150CC8A2" modified="2013-10-16" version="1">MTHSSQTFSCDILDDIWIWYSCLRITFLNRTKILRSTSFFDAPPCVIQSHMTESNDPNRFITINSSLNSMFNIQAWVNPSTPFDQNVFLMPLGLVIKPVTESFNSPRIDKYSIKEFACSRSSAAAERGGFRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDE8</accession>
    <name>T1GDE8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001333-RA">
        <property type="protein sequence ID" value="MESCA001333-PA"/>
        <property type="gene ID" value="MESCA001333"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1361816_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577287" evidence="1">
        <location>
            <begin position="17"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001333-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="22636" checksum="CB8DB615CFEDBDF7" modified="2013-10-16" version="1">MTKIIGFIAIVGVVGASQLHTRVRPRSAYDDLVDPIYPPLKDYHQFSSQGYDIGIPSSEEYAADFPQSFDHQISGHTNVNTDLSAHTLDFQNEFDTLTADYKPIHEPNVEDYETQQFENKVFHQVPAPQLGKSYNIGFDTSAQDFSELASTPHLESHGRSIESVHKNLFRPADPYESAYNTGQLLKYTESVLKSKNLAIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDU6</accession>
    <name>T1GDU6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001496-RA">
        <property type="protein sequence ID" value="MESCA001496-PA"/>
        <property type="gene ID" value="MESCA001496"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707627_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001496-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8166" checksum="16A328D5A78575C3" modified="2013-10-16" version="1">MVSYGTIISSCNFITLSIDIEALYLPSVNILVEPIPFCCWENFSKGTSPYNRGLRSSINHRVMSTYVSTIFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE55</accession>
    <name>T1GE55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAFII28-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE55"/>
    <dbReference type="STRING" id="36166.T1GE55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001613-RA">
        <property type="protein sequence ID" value="MESCA001613-PA"/>
        <property type="gene ID" value="MESCA001613"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088696_0_1_1"/>
    <dbReference type="OMA" id="ASKQFFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000025">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="7">
        <location>
            <begin position="93"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22933" checksum="C80819540BCA90EC" modified="2013-10-16" version="1">MEDILSSDYSQDVKHNSSTDNDSFLEDSKDENIKSEDEEKADPKTDADDEESARIRQEIEEELAEALRQDAIKKQQQKSRREMEEEERERMQVLVSNFTEEQLDRYEMYRRAAFPKASVKRLMQTITGCSVSQNVVIAMSGIAKVFVGEVVEEALDVMEENGETGGVQPKYLREAVRRLRNRGRLPIGKYQKPYFKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GET3</accession>
    <name>T1GET3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AGC-kinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02059394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GET3"/>
    <dbReference type="STRING" id="36166.T1GET3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001854-RA">
        <property type="protein sequence ID" value="MESCA001854-PA"/>
        <property type="gene ID" value="MESCA001854"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_26_1"/>
    <dbReference type="OMA" id="DWNALEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="6">
        <location>
            <begin position="16"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001854-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9803" checksum="B0F78E1FE0FBBAA6" modified="2013-10-16" version="1">GGGQAPEEIKGHSFFRAIEWEKVENREEEPPFVPALKGRKDLAHFDTDFTAATTDLTPTDKLFMMNLDQTDFIGFSYLNPEFLTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEX3</accession>
    <name>T1GEX3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclin-dependent kinase 2-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDK2AP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02045821">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GEX3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001896-RA">
        <property type="protein sequence ID" value="MESCA001896-PA"/>
        <property type="gene ID" value="MESCA001896"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962098_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017266">
        <property type="entry name" value="DOC_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22607:SF3">
        <property type="entry name" value="CDK2-ASSOCIATED PROTEIN 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22607">
        <property type="entry name" value="DELETED IN ORAL CANCER 1/CDK2-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09806">
        <property type="entry name" value="CDK2AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001896-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="13999" checksum="E2FE9E693A57F8CF" modified="2013-10-16" version="1">MQPEQEPRIQISPIHHNDEYIRQKSSSNHSSSNASASAAYQMAALSAMSSGLSISTGNSNPNGPSKYAQLLEVIEEMGRDIRPTYQGSRXXXXXXXXXXXXXXXXXXXXXXXXXXEFNGTPEKGYSPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF36</accession>
    <name>T1GF36_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF36"/>
    <dbReference type="STRING" id="36166.T1GF36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001965-RA">
        <property type="protein sequence ID" value="MESCA001965-PA"/>
        <property type="gene ID" value="MESCA001965"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_508641_0_0_1"/>
    <dbReference type="OMA" id="NCDHENE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052424">
        <property type="entry name" value="Kielin_Chordin-BMP_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46698">
        <property type="entry name" value="CROSSVEINLESS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46698:SF3">
        <property type="entry name" value="TENECTIN ISOFORM 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFC" evidence="5">
        <location>
            <begin position="180"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="469"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001965-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="536" mass="60414" checksum="C4BCFD6499538A8A" modified="2013-10-16" version="1">PLQESTDLQEYSEGCYYNYNHYNEGDRISTNEPCLNCTCHNKMLMCYLRVCPFTKPIGHDCVVEKREDQCCPIITCPEVPVELNHQSDLSTELAIPEIPSNPNKPCELCYCIKNQTSCLMQECTLHIDGCQPIYNKNSCCPVRYNCDHENELIEDLSTTTTTVRPTTGFMLTTVSSLTSADCYHDDKTYPDGSLIKTSDSCENCYCMRGDIVCAVQKCEVPTMEVEGKVCKAGEKLEGECCPSNYICEDIDRNDPEKIHKISQEDVELQQHIDEENVYTTEEKDDDAKDKEDSINIDETTVSTSEENYVTETTISSIDSNKDILTNESQENKTKTESNLIFNTDSPDQIKDDTLEDIEDIPISIPGEGNCLVDDETYSNNTRVPTSNECEEYCLYNTNKLECCPVYICDSNNTQYLPPKEDNLLHNVDSTEKSVDENIEITTLSEDHLPTSASDVLEIEKTTHKSLEVSSTESHLVDEKLESSTDISDISSSEKIENDFESSGSGEIYTTTTKSSIYEEHAKETENTSEEKENVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGB6</accession>
    <name>T1GGB6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGB6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002430-RA">
        <property type="protein sequence ID" value="MESCA002430-PA"/>
        <property type="gene ID" value="MESCA002430"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2102977_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002430-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13008" checksum="30DBA3882E34784F" modified="2013-10-16" version="1">PIIQNNRPNKKKGFKNKPNKKPNQSSDVPVADFDYKSANFNMFKGGAQKVQGTQLKNGKFQAKARQKHQKCSGVMLSTSCPRLPTNLETEILILFPWIPILGSKASKGLRYVKFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGE0</accession>
    <name>T1GGE0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gamma-glutamylcyclotransferase AIG2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002458-RA">
        <property type="protein sequence ID" value="MESCA002458-PA"/>
        <property type="gene ID" value="MESCA002458"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565003_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002458-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9360" checksum="133A1E2F010DA999" modified="2013-10-16" version="1">MKNQGTRKGIGTLKDYMLGFNSWINVWDGGVATIMKKPGRNVMGAIWEIDLDLIKSLDIQEAYYNRFLVNIELDSGEIVKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGH7</accession>
    <name>T1GGH7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02084078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGH7"/>
    <dbReference type="STRING" id="36166.T1GGH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002497-RA">
        <property type="protein sequence ID" value="MESCA002497-PA"/>
        <property type="gene ID" value="MESCA002497"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_919165_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF303">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GABA TRANSPORTER INE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="255"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002497-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="303" mass="34238" checksum="330BD3E787452D9B" modified="2013-10-16" version="1">MGRALTLEGANEGLEFLFRPDWKLLGNANVWINAASQNFNSLGIAFGSMISFSSYNKYSNNVLHDTIAVSMVNAVTSILVGIFAFATLGNLAWEQNSSVSSVISDGPGLIFVVYPQVMAKMPYAQLWAVMFFFMLLCLGLNSQFAIVIYINYILSLLLITYFHYYKQISIYMGGGIGWMFTSFSLVCIPAFAIFVIYHQTDKKFCEKILSSIRPRIYECKICGEHHCEHDFPEEEQFSQEPSTPIYTPKILLRSPPIPHSKDKTYNPLIEKPSSSEIEENETNFTVQNTVINDDTTKSKDEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGN1</accession>
    <name>T1GGN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02091589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGN1"/>
    <dbReference type="STRING" id="36166.T1GGN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002554-RA">
        <property type="protein sequence ID" value="MESCA002554-PA"/>
        <property type="gene ID" value="MESCA002554"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2892483_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF18">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002554-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7077" checksum="D47B17EEA53B3999" modified="2013-10-16" version="1">NTKIYISFFRGFGFITFSDSSSVDKVLSQGTHELDGKKIDPKVAFPRRAHPKVSTNMVLLHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHE1</accession>
    <name>T1GHE1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynein heavy chain hydrolytic ATP-binding dynein motor region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHE1"/>
    <dbReference type="STRING" id="36166.T1GHE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002837-RA">
        <property type="protein sequence ID" value="MESCA002837-PA"/>
        <property type="gene ID" value="MESCA002837"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_919164_0_0_1"/>
    <dbReference type="OMA" id="ESIHEQM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF72">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 3, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="1">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="2">
        <location>
            <begin position="211"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002837-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="34388" checksum="AADBF4E4A173A82E" modified="2013-10-16" version="1">MLTFEENLTISAMISAEQEEVALIRRIDPNSANGLVELWLKDVETTMLESIHEQMRLAWENYYKTTRINWVLSWPGQVVQSISCMAWTYETNLTNLLPTVLGKLNLIAVLLSGRIAIETNDLSNYLAKCVAQISELVYLVRTDLSSGERIAIEALIVLDVHDKDVVKYMLDEGVSSIAEFDWISQLRYYWKVNDENMEYVCVSMVVTDVKYGMEYLGNVPRLVVTPLTDRCYRTLMGALKLYLGGAPEGPAGTGKTETCKDLAKAVAKKCVVFNCSDGLDYKALGKFFKVCIFFRNIFIRFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHH9</accession>
    <name>T1GHH9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02005212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHH9"/>
    <dbReference type="STRING" id="36166.T1GHH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002881-RA">
        <property type="protein sequence ID" value="MESCA002881-PA"/>
        <property type="gene ID" value="MESCA002881"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_669650_0_0_1"/>
    <dbReference type="OMA" id="FEGSAIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="82"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="198"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="285"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002881-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="414" mass="46901" checksum="A4697E8258ED5ABC" modified="2013-10-16" version="1">MAPIILYHNEISAPSRFVLMVIKSLNFDVKSKEQLKPEFLKINPQHTVPTIDDNGFVLWESIAIAEYLIDTYAPGNSLYPKDTKERALVNQRLLFFVATLLIRVRVITHPIMKDGEKDIPESKRTMLYEAFKLADGFVSRNDWMAGNQLTIADMAIFSVISTIVAFGADISEYKNLCSWVERCKAIAGYDENMEARMAKIILYHNVLSSPSRFALLAIRNLGIDVEIRIVDLMKKEQLKPEFLKINPQHTVPTMDDDGFILWESGAISQYLAETYIPDSPLYPSDTKKRAIINQRLQFFLGTLLPRMRQVTHPIMFEGLKEVPEAKRDQLYEAFELLEGFLTTSAWVAGCNVTLADLAVLAVITTIVSFGADISGFPKLSAWAEKCKSLPGYEENMEGVKAFTKLFKNFYDGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI19</accession>
    <name>T1GI19_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI19"/>
    <dbReference type="STRING" id="36166.T1GI19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003086-RA">
        <property type="protein sequence ID" value="MESCA003086-PA"/>
        <property type="gene ID" value="MESCA003086"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009661_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003086-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13564" checksum="B2FDDAA1935D365D" modified="2013-10-16" version="1">MSTPIELNDRETLIKSASTSLNSKNIKVIQSLGGTIEDTEMINGLVFTSRASGSNAPKKVEKAKIGLIQFCISPPKTDMDHSVIVSDYAAMDRVLKEERAYILNIVKQIKKAGCNVLLVQKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI54</accession>
    <name>T1GI54_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI54"/>
    <dbReference type="STRING" id="36166.T1GI54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003121-RA">
        <property type="protein sequence ID" value="MESCA003121-PA"/>
        <property type="gene ID" value="MESCA003121"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_7_40_1"/>
    <dbReference type="OMA" id="HEMCRIA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF611">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE TRANSMEMBRANE RECEPTOR ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003121-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11862" checksum="9F9B79C7A616643B" modified="2013-10-16" version="1">MIARDVAEGMKYLQNMNIIHRDLAARNILIDNSRAKISDFGLARVADSYGFYKVQTMDREIPVKWYSPETIETNTFSFYSDVWSFGVTLFEMFSRGETPYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIN0</accession>
    <name>T1GIN0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIN0"/>
    <dbReference type="STRING" id="36166.T1GIN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003313-RA">
        <property type="protein sequence ID" value="MESCA003313-PA"/>
        <property type="gene ID" value="MESCA003313"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1541863_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003313-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="18710" checksum="19896EC78250460F" modified="2013-10-16" version="1">MPSQTSFDVETTGFADFTKFSEEVNECNSKPSTATPPRPTVSSIQSNQRVSAFEVYKKPTITPRSSQSPVELRQALPPPQIYPPSLDYEKKVSAINETLRNLEQNSLLLRLCHDLSEELLSVQHRKEEIRSKLETASGVTANSGATSTSIVNSGGITFVTANSTGGSGGSASMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIR8</accession>
    <name>T1GIR8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIR8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003353-RA">
        <property type="protein sequence ID" value="MESCA003353-PA"/>
        <property type="gene ID" value="MESCA003353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624820_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8905" checksum="5DD431A7B0F4F620" modified="2013-10-16" version="1">MKVGNPCEGGCWETMMRTVKRVLCYSSRPLTHVPINVTEDEPLTQNHFILGSANRVQTPSIYDKPNTNLRNQRSLWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIU4</accession>
    <name>T1GIU4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003379-RA">
        <property type="protein sequence ID" value="MESCA003379-PA"/>
        <property type="gene ID" value="MESCA003379"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149651_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003379-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="28525" checksum="97CD99F7FD33B9FE" modified="2013-10-16" version="1">MIFRREEKDPIKMCPAIEELILSRNEEIWKRFEENNANTEQYSYILKKFKYTQNSVKWPFKSESETLKNLVPVDLVLTFGKPIEKILKTIFVLPLNMYGLIGQIEKKLENSVEKFVSVSANLPENQIICTFNSDLSIIFEFKDNFGTSEREFLANVFKKWIAVKPKRIIFKNFPFLTHFSVSLLRMLTSCYKSFTADLNCFVELNDLETEKCKDFEKLENIFKNQSFHLIMCFTELTKVNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIW0</accession>
    <name>T1GIW0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02195296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIW0"/>
    <dbReference type="STRING" id="36166.T1GIW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003395-RA">
        <property type="protein sequence ID" value="MESCA003395-PA"/>
        <property type="gene ID" value="MESCA003395"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_9_1"/>
    <dbReference type="OMA" id="FITHRYI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003395-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="12254" checksum="B087B2384B7EE2FC" modified="2013-10-16" version="1">ALVVRFITHRYIGEYTNLEKYTYNHIKTVDNDNVRFEVLDTAGNHCNDLEKMGLRSHIKWADGFVLMYSISDLCSFNECSRLKFLINYSKRRKKSKVRLFLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ84</accession>
    <name>T1GJ84_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02073650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ84"/>
    <dbReference type="STRING" id="36166.T1GJ84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003528-RA">
        <property type="protein sequence ID" value="MESCA003528-PA"/>
        <property type="gene ID" value="MESCA003528"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003528-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="24084" checksum="509E03D636E4FCB6" modified="2013-10-16" version="1">MTSSKSELRKLSEDSLTQPAEKVFDIICKLGEGSYGSVYKAIHKESSSVVAIKLVPVESDLHEIIKEISIMQQCDSPYVQTLKEEEIATILSDTLKGLEYLHLRRKIHRDIKAGNILLNLDGYAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIEEIGYDCVADIWSLGLDVNSKGNLGILFANLEPKPYFYIIKQQDGHQDLGHRDSDASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJM2</accession>
    <name>T1GJM2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJM2"/>
    <dbReference type="STRING" id="36166.T1GJM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003672-RA">
        <property type="protein sequence ID" value="MESCA003672-PA"/>
        <property type="gene ID" value="MESCA003672"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072885_0_0_1"/>
    <dbReference type="OMA" id="PDWIGAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003672-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="21369" checksum="8F7C0CF41E209D78" modified="2013-10-16" version="1">MNSYFEQTGFYGHPHQTTGMGMGSSGHHDQTSYRGLPFSLGMPYTNPHLQRSTQDSPYDASISAACSKIYDGSYKQDCAKAANLSGGSGDGTNGYKDVWNTSGSNGSAGNPGSGSGSGSGSGPNNSVPVRPSACTPDSRVGYMDTSGGSPVSHRGGSANAVTGSAWNANCTISGAAAAAAAQSTGGLHQANHTFYPWMAIAVQEKLQWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP8</accession>
    <name>T1GJP8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02079003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003698-RA">
        <property type="protein sequence ID" value="MESCA003698-PA"/>
        <property type="gene ID" value="MESCA003698"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592499_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003698-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9132" checksum="84AD6E543BE965E3" modified="2013-10-16" version="1">MCELEFCIRTRILAPVPISDIMRTLATVPNSDEKFLNMHPHSLEPYFLQDGPKHTYLAFSFSEGNKALSSLIAIMFIRCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK26</accession>
    <name>T1GK26_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK26"/>
    <dbReference type="STRING" id="36166.T1GK26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003840-RA">
        <property type="protein sequence ID" value="MESCA003840-PA"/>
        <property type="gene ID" value="MESCA003840"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984114_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF24">
        <property type="entry name" value="THYMIDINE KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003840-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14909" checksum="FD59E4BE39635C59" modified="2013-10-16" version="1">MLEIHLKTIKSSIKVMERSIYSAKEVFTKSLLDDGYVSQVEYNKMIKKCEDIIQSNEITTDMIVYIRTDPSVSFSRIKERGREEEFTITFKQIEKLHNLYEDFIKSNGNQGYRDVLKDFKLLLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK94</accession>
    <name>T1GK94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Eukaryotic peptide chain release factor subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK94"/>
    <dbReference type="STRING" id="36166.T1GK94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003913-RA">
        <property type="protein sequence ID" value="MESCA003913-PA"/>
        <property type="gene ID" value="MESCA003913"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2981419_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003913-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="58" mass="6454" checksum="092937EE30A8559C" modified="2013-10-16" version="1">MISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKEGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKL6</accession>
    <name>T1GKL6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKL6"/>
    <dbReference type="STRING" id="36166.T1GKL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004042-RA">
        <property type="protein sequence ID" value="MESCA004042-PA"/>
        <property type="gene ID" value="MESCA004042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1125650_0_0_1"/>
    <dbReference type="OMA" id="CCISVIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1210:FF:000002">
        <property type="entry name" value="MOXD1 homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1210">
        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.310">
        <property type="entry name" value="Copper type II, ascorbate-dependent monooxygenase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036939">
        <property type="entry name" value="Cu2_ascorb_mOase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008977">
        <property type="entry name" value="PHM/PNGase_F_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF40">
        <property type="entry name" value="MOXD1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49742">
        <property type="entry name" value="PHM/PNGase F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588378" description="DOMON domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="2">
        <location>
            <begin position="41"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="28377" checksum="C7463516551A3043" modified="2013-10-16" version="1">MDSSSFIKRLISSLAVILTINSLCPSMVSAIHWDHAVDLNEDFRILWTIINQDITFEIQARTLGYVGFGFSPDGNLPGSDIAIGWVDRGQTYFQDRHIKSYGDTEPVVDPSQDYILMLGYENSTHTVLRFRRKLDTCDTAHDIPITNDTMRIIYMYHEGDPRHGSVAPGTLPNPHDAVRPYRSVVLTQKFQPKIQRGDERIRMLELRNEDVELPDGDDTLYWCKMFKLEDINRKHHLIRVSIQTGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKY9</accession>
    <name>T1GKY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3'-phosphate/5'-hydroxy nucleic acid ligase</fullName>
            <ecNumber evidence="1">6.5.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02193881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKY9"/>
    <dbReference type="STRING" id="36166.T1GKY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004172-RA">
        <property type="protein sequence ID" value="MESCA004172-PA"/>
        <property type="gene ID" value="MESCA004172"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093621_0_0_1"/>
    <dbReference type="OMA" id="IERISDC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="17940" checksum="C8107C9A648A11CD" modified="2013-10-16" version="1">MVVRSYDEELSYIERISDCCWRIKKGFQPNMNVEGCFYVNKKLEALMFEELRNSCRPGMTGGFLPGVKQIANVAALPGIVGRSIGLPDIHSGYGFAIGNMAAFDMENPESVVSPGGVGFDINCGVRLLRTNLYEKDVQAVKEDLAQSLFDHIPVGVGSKATCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW8</accession>
    <name>T1GLW8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab3GAP regulatory subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101683">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GLW8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004533-RA">
        <property type="protein sequence ID" value="MESCA004533-PA"/>
        <property type="gene ID" value="MESCA004533"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_608731_0_0_1"/>
    <dbReference type="OMA" id="WNAHIKI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029257">
        <property type="entry name" value="RAB3GAP2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14656">
        <property type="entry name" value="RAB3GAP2_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3GAP regulatory subunit C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Rab3GAP regulatory subunit C-terminal" evidence="1">
        <location>
            <begin position="131"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Rab3GAP regulatory subunit C-terminal" evidence="1">
        <location>
            <begin position="227"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004533-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="450" mass="51828" checksum="DB9B49C18D18026F" modified="2013-10-16" version="1">MILECPCALRGLVFAIVCKHISVRFLRKDENDMSQDEGNWEQVSHEAAQWTLLIGKLDDIAILGAILSSSQSSSLSSKMVKISETYEKPNCSLKALLCGSKSIVSELTANWLINSQIDPKLIVENDEDLPEDPVLIHLAHLRKHFPFSLESGMILCQMVWEFMSHWSKNLSSLEHLEAGLKCLDAFRKQDQALKHGLCCLVWNAHIKIPLEATKKLINXLDAFRKQDQALKHGLCCLVWNAHIKIPLEATKKLINKAGRLPKDKLCQQDLGISVTAVADFLSYSIDFLENHFQASMEFDQQKDIKHEEILSGTQGEQFQLQFLALQQNHAILEILKLHIEMCKVLHLIAVFQLKLPKPMNFLFDAMSNQTFFFEINKELQYYLPIPDKILQNQRKEFLCKAVTASVDLVREDLEHLYIEDHEDYMNKIENLADMWKLDKKRIKRHQVLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM27</accession>
    <name>T1GM27_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072576">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004595-RA">
        <property type="protein sequence ID" value="MESCA004595-PA"/>
        <property type="gene ID" value="MESCA004595"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1291821_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF149">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C X DOMAIN CONTAINING, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="95"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004595-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="214" mass="24996" checksum="BFEF5A49038A0DB2" modified="2013-10-16" version="1">MFLRVEETVDHNSWYFGEFNKSSRCHSVWAVYLNSSGRIVASTCLMVHPRWLNNLLPNIGFRRFRDLIIPGTHDSASYKEGFEIPQVELPVTRYSLTQDDTILGQLYQGARYLDIRPSFYKNLPNKWYVNHGITIQQTLDNVMDQVKKFVNETGEPVIFGLKEFPVGFNTIDIHRNLVQYMESYFGNYIFKPKDMDEPWKFTLNDILGGPQQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM59</accession>
    <name>T1GM59_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02005828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM59"/>
    <dbReference type="STRING" id="36166.T1GM59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004627-RA">
        <property type="protein sequence ID" value="MESCA004627-PA"/>
        <property type="gene ID" value="MESCA004627"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1120904_0_0_1"/>
    <dbReference type="OMA" id="FQEFRVK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004627-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="29812" checksum="CEFCBE102EBF2F53" modified="2013-10-16" version="1">MEEIDGNERVLHLIFPDGLPENLQDNPELQIEQLKKEQSRIQEEHKNLVEQTQDLAVSNYKTFIQTAEKSKDLSQDFGNTEKQVENLIEKFPKCEENCREFLAKSSETIEKRRLNALTLKKNSQLLEILELPELMEKCVDEEKYEEALELAFHVHRMGVKQENIPIIQRIVGIVQTHWHKMLLKLLDQLKTDLQLPKCLQIVGFLRRMQAFDNKELKLKYLQARDAWLEKCLEAIPNENHDLYAPNLSIPLMIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMV3</accession>
    <name>T1GMV3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004887-RA">
        <property type="protein sequence ID" value="MESCA004887-PA"/>
        <property type="gene ID" value="MESCA004887"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707609_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004887-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8288" checksum="B1359116DE928FE0" modified="2013-10-16" version="1">MVEAFPGSCLLKKNKNPPWWNREIATKSIDRRSLIITKREHSALLFLLLQNQLRTPASLGEAKIEIVLTSSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMY1</accession>
    <name>T1GMY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMY1"/>
    <dbReference type="STRING" id="36166.T1GMY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004917-RA">
        <property type="protein sequence ID" value="MESCA004917-PA"/>
        <property type="gene ID" value="MESCA004917"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008163_1_2_1"/>
    <dbReference type="OMA" id="CEENECE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="13">
        <location>
            <begin position="224"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="13">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="4"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004917-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="296" mass="32973" checksum="894CCDC73885275F" modified="2013-10-16" version="1">MFTCGNGRCINNLWVCDHDNDCGDGTDEGKFCNSKYKTCSPEDFTCQNFKCIRKRYRCDGEDDCGDHSDEVDCKKENVTCPAGKFQLSDCSDDSDEPAHCNVDECAKVEIHQCGHKCVDTLTGYYCDCNQGYKLLADGKACADIDECLEKPGVCSQHCSNTPGGYYSISVDWVADNLYWAEVDRTGSKPRGRIMVAKTDGRYRRAIVSAGLEIPTSVVVNPQLGRMYWSDAGSAPKIEVSWMDGSKRRPVITEAIRHPAGLTIDYAQDHTIYWVDTKLNTIESMKPDGSIRKTIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN16</accession>
    <name>T1GN16_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN16"/>
    <dbReference type="STRING" id="36166.T1GN16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004955-RA">
        <property type="protein sequence ID" value="MESCA004955-PA"/>
        <property type="gene ID" value="MESCA004955"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519025_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588413" evidence="1">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004955-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9244" checksum="D02DE86F4CF35BB5" modified="2013-10-16" version="1">MNTFNTLLVLVGFVAFVQSASITKEMQEKFMVVAKECQGQTGASDADLANLIKHNPTGTKEGKCMLSCIMTKVDTQDSNGKLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNP5</accession>
    <name>T1GNP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Required for pre-mRNA splicing as component of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02030776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNP5"/>
    <dbReference type="STRING" id="36166.T1GNP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005203-RA">
        <property type="protein sequence ID" value="MESCA005203-PA"/>
        <property type="gene ID" value="MESCA005203"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022457_1_1_1"/>
    <dbReference type="OMA" id="KIPTANW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22718">
        <property type="entry name" value="FHA_SNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000008">
        <property type="entry name" value="smad nuclear-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FHA" evidence="13">
        <location>
            <begin position="106"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="26544" checksum="A7AF98492AC4E766" modified="2013-10-16" version="1">MRGGESRSPRRPKNFPNRRNDEDDKDYEWGKKDSNEKKDDEKPVEKEKPNFGLSGKLAEDTNKVNGVVVKYSEPLEARKPKRRWRLYPFKGEKPLPTMYIHRQSCYLVGRDRKICDLAVDHPSCSKQHAALQYRLMPFEREDGSTGKRVRPYLIDLDSANGTFLNNKPIEARKYYELMEKDVIKFGFSSREYVLLHENSKEDQEDDDVNEEVQVVETDEKPIKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP21</accession>
    <name>T1GP21_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear inhibitor of protein phosphatase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Protein phosphatase 1 regulatory inhibitor subunit 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP21"/>
    <dbReference type="STRING" id="36166.T1GP21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005337-RA">
        <property type="protein sequence ID" value="MESCA005337-PA"/>
        <property type="gene ID" value="MESCA005337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069628_0_0_1"/>
    <dbReference type="OMA" id="HREMPPP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22674">
        <property type="entry name" value="FHA_PPP1R8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000012">
        <property type="entry name" value="Nuclear inhibitor of protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="40"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="283"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="343" mass="37573" checksum="184642B7215460BE" modified="2013-10-16" version="1">MANSYEIPSWAGKAPVGHHLDVLKDDKLIQKLMIDEKKCYLFGRNPQMNDVCIDHASCSRVHAALVYHEFLKKSFLVDLGSTHGTFIGSIRLEGHKPTQLQIDSVFSFGASTRKYILRERPNATIPMGSSLMDDMQVEGGEGALLGLPESQTELDDSIRKPNTNSKRKRKHITFNDEEIIINPEDVDPSVGRFRNLVQSTVLPAKRMKYETNSMGIPHFTKPATIQPIISGADKTLQSSLYHGIPDAAGASGSKPHHPMDIDVTQVALGSKLGLLLPNPAPDVNPEEPISVPKVQFSPAPVTSGAHTYEESMMVEAPSTSGGPQKKKYAKESWPGRKPHAAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPP2</accession>
    <name>T1GPP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPP2"/>
    <dbReference type="STRING" id="36166.T1GPP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005578-RA">
        <property type="protein sequence ID" value="MESCA005578-PA"/>
        <property type="gene ID" value="MESCA005578"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1157570_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="137"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005578-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="28266" checksum="5CD821E88EDADF38" modified="2013-10-16" version="1">MYVNLIENLEIYKQLSQDKSFDIKPQVKLLINNAEFIKKIQTEIDIQNPICELINVCQKSSTNVGDAAKLWLNLKPHLSFNKKQKSSFDERVKTILTDCALSSYFLNPYNDLSVLSTEHLRLITNFMIKELPGNFIDQYLEYKKLDGIFKSYKAKASNVFDYWSLIQNRYPEIARIALRLLKIPASSAQLERVFSAWQHQHSELRNRLNFENSTMLMHIDYTYMLLDDENLDYDLEETEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPU7</accession>
    <name>T1GPU7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Collagen IV NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005641-RA">
        <property type="protein sequence ID" value="MESCA005641-PA"/>
        <property type="gene ID" value="MESCA005641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123904_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12103" checksum="084248F2CAF50DF5" modified="2013-10-16" version="1">MDSETKNIIQLLPKKTFSIKQASKRKEWNNSIICLSTRKETRVTAKTTEESHPINGGAGQHGSGPQGPQGPQGPHGPQGPQGPHGPQGPQGPHGSLHGQFPQRDPGLYGIGFAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ19</accession>
    <name>T1GQ19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02191047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ19"/>
    <dbReference type="STRING" id="36166.T1GQ19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005717-RA">
        <property type="protein sequence ID" value="MESCA005717-PA"/>
        <property type="gene ID" value="MESCA005717"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_7_0_1"/>
    <dbReference type="OMA" id="TYSPFFW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="8">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005717-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="550" mass="61719" checksum="FF23FBE0FDE5E1EA" modified="2013-10-16" version="1">MYITAGSAGSVVANRLSANPNWKVLVLEAGGNPPIESEIPSMPYLMQKTDATFNYFTDPSPDYCLAYKNNSCYWPRGKMIGGSGAINAMVYVRGNKEDYDNWERLGNKGWGWNGVLPYFEKSRKFVDGSGLALNRYDESDKILEIVKKSALELGNKEVVDFDGLNDIGYTETPSTQENGRRMSTGKTYLAKAKDRSNLHVIKNAVVKKINLSRESKTADSVTFIYQGSKEFTVKKEIICSAGAVDTPKILLHSGIGPREQMKKFGIPIVKDLPVGQHLQDHSMIPVMFKIQEKSAKPFDLKQKLVDLQNYLLENKGPIGRLGLSLVGFVNTDPDSDSPYPDIGTYHLPIRRQDPFIDVFLNAFNFKEEVVKPVRDAHDKAHLVVVYLCIQRPTSIGEIKLNSRDYNEDLSIVPNYLGTDYDVDVMLRGLKYQLSLENSKTFKENGGEFIKLNIPECDDFEFKSDKYLRCFMKYMITTIYHPIGTAKMGPDSDPLAVVNSNLKVKGINNLRVIDASIMPFMISANTNGPTIMIAEKGTDFIINDWNAKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQB5</accession>
    <name>T1GQB5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQB5"/>
    <dbReference type="STRING" id="36166.T1GQB5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005819-RA">
        <property type="protein sequence ID" value="MESCA005819-PA"/>
        <property type="gene ID" value="MESCA005819"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_2_4_1"/>
    <dbReference type="OMA" id="FCIVASK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000103">
        <property type="entry name" value="Troponin C Ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005819-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="14806" checksum="486FEE5303AE472B" modified="2013-10-16" version="1">MADELGKEQIELLKNAFNAFDQEKKGYIDTVMVGAILGMLGHPLDDDMLAEIIAEVDEDGNAEAMQQELKEAFRLYDKEGNGYITTAVLREILKELDDKITNEDLDMMIEEIDSDGSGTVDFDEFMEVMTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQY7</accession>
    <name>T1GQY7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CAQQ02156475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQY7"/>
    <dbReference type="STRING" id="36166.T1GQY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006053-RA">
        <property type="protein sequence ID" value="MESCA006053-PA"/>
        <property type="gene ID" value="MESCA006053"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_2_0_1"/>
    <dbReference type="OMA" id="RECTANA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF38">
        <property type="entry name" value="HISTONE DEACETYLASE 6, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="93"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="446"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006053-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="693" mass="78831" checksum="BB288CE1DA53D4FD" modified="2013-10-16" version="1">MVKSVESRKPNAALLEAKRRAKNLKMLEQEQIVEPVKDVFSKALSSLKMVRSETGIIYDDSMDQHKCFWDEEHQERPERFTEIIKRCQELDLLDKSTSGVTDQEHLEDTSSNYNAIYIHPSTFDLSLLSAGCTIDLVDNIIKKKVQNGMAIIRPPGHHAMKAEFNGYCFFNNVAIAARHALDVLNLKKILIIDWDAHHGQGTQRFFYDDPRVLYFSLHRYEFGEFWPNLRESDYDWIGEGSGKGFNFNIPLNKKFMQNEDYLSVFQQILLPVAFEFQPELVIVSAGYDAAFGCPEGEMALTPAFYSHMTRSLMHLADGKLAIVLEGGYCVRSLAEGATLTLRALLDFPSPTLVDKWEVPSPEICETILNCIYVHRPYWKCLNVQDTYTLEELNNKNPQPDLHKVVQVYKGKLYLKKTTDLECSKNKLCYVYDDKMCEHKNVDEEGHPECPERIKKIKSKFEEFGLTQRMQLLPSRIATTDEVCLAHTRLHLNFVRRLTPKANLKEIGSKFNSVYLHEKTFDCATLAAGSVLNVVDNILTNQFRSGVCVIRPPGHHAEPDQPHGFCIFNNVAIAAQYAIRNGVNKVLIVDWDVHHGNGTQHIFENNPNVLYISIHRYDNATFFPKKTDANYDVVGKGAGEGFNVNIPWNKKGMTDMEYIMVMQQIVLPIAYEFNPELVLVSAGFDAAIGDPLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQZ3</accession>
    <name>T1GQZ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF753 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006059-RA">
        <property type="protein sequence ID" value="MESCA006059-PA"/>
        <property type="gene ID" value="MESCA006059"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714319_0_0_1"/>
    <dbReference type="OMA" id="TTTNSEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008472">
        <property type="entry name" value="DUF753"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721">
        <property type="entry name" value="GH09876P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05444">
        <property type="entry name" value="DUF753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577634" description="DUF753 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="2">
        <location>
            <begin position="248"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006059-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="387" mass="42798" checksum="AADD441EECD2DD1E" modified="2013-10-16" version="1">MSRVKQVTFLVFIALFVQGLQSTCIECNSDNESACISSPNAVKTTCPYNNCYSYQHPETKYVERGCLSNGIPEVCSNLENCNICSNADLCNKESFKVDCLECNSATNADCKDKPEAITSFCQSETCYFRENIENVVSRGCLSVDTEQSCVEDKDCTSCKDEQHCNIKQDTTPECFSCQGDGCSEIDFATQTRVKCNEGSKCITQLNNQGLVVRGCTQSAQEKCLDENTCKICDGRLCNGEIFPTDRLKCIQCNSTAQGDECSLASEKFSRTCNIYNKEDQCFTYADEKGLLYRGCASDKNQACTEDNCLKCSGDNCNTNGLQVPNKLKCIKCDSSKGEECEADKIKAEAVTCTGNHNLNERETCFVQKVTSEDNKVTTKRGCFFELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSB3</accession>
    <name>T1GSB3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSB3"/>
    <dbReference type="STRING" id="36166.T1GSB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006566-RA">
        <property type="protein sequence ID" value="MESCA006566-PA"/>
        <property type="gene ID" value="MESCA006566"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1664794_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="8"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006566-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="20167" checksum="565C8D07327D935C" modified="2013-10-16" version="1">MDLKLTLTIVVASRLVYRKGIDLLAEVLWRFKDRSNVRFLIAGDGPKRDLLEEIREKANMQERVDIIGAVEHSKVRDVLVKGDIFLNTSLTEAYCMAIVEAASCGLQVVSTKVGGIPEVLPAKLIIMKEPTVDDISEGLEEAINIHYTGDSSKILCPYKRNEMVKNLYNWENVTCRTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSQ0</accession>
    <name>T1GSQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02060933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02060934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02060935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02060936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSQ0"/>
    <dbReference type="STRING" id="36166.T1GSQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006711-RA">
        <property type="protein sequence ID" value="MESCA006711-PA"/>
        <property type="gene ID" value="MESCA006711"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006771_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06669">
        <property type="entry name" value="PDZ5_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000070">
        <property type="entry name" value="Multiple PDZ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051342">
        <property type="entry name" value="PDZ_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964">
        <property type="entry name" value="MULTIPLE PDZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964:SF92">
        <property type="entry name" value="PATJ HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="182"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="349"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="494" mass="53647" checksum="805C0A77E778A9DB" modified="2013-10-16" version="1">WYIEIIDGQSLQGFSNHQAVELLKKSGKIVNLKLERYLRGPKYEQLQQAIAANDKVPSSIPSTPSRSLALHYPSQIPQHASSDDFSTSEIQSDVFSPDLISEHSNLKLVNSIGKKTEQPSKIPKKKIIMDAIVAPVTTINENSEKIEISKSKYYVDPMTIEIESSIIRKWRSIIGSEVEIIVAQFQKLTATSGLGISLEGTVDVEEGPVGQNGKLKSGDELLEVNGQRLLGMNHLEVVAILKELPQDIRIVCSRGQQPVFQSSIVMGPLGGSMKKLNSNSNLDNVLPISSDRLVKAKSDGSLATTSSSVIGFSSVLGGCDPDDSFSNKLKSRSLEPLTGLAMWSSEPQIIELIKGDRGLGFSILDYQDPIDPNDTLIVIRSLVPGGVAQNDGRLIPGDRLLFVNNINLENASLDHAVQALKGAPKGIVKIGGIVKIGVAKPLPLQDNLIKTEQLSDNSGISNASTAEPFDGKKNKKLNKCYNTVYLQIIFNYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSR0</accession>
    <name>T1GSR0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSR0"/>
    <dbReference type="STRING" id="36166.T1GSR0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006721-RA">
        <property type="protein sequence ID" value="MESCA006721-PA"/>
        <property type="gene ID" value="MESCA006721"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402165_0_0_1"/>
    <dbReference type="OMA" id="EERTRMH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577671" description="Cathepsin propeptide inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="2">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006721-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="11205" checksum="DE7A5372AB9C63EF" modified="2013-10-16" version="1">MKVYLFCIFVLIFLGFYTVSSVEISDEEWTNFKEKFNKSYDEKEDLERREIYQKSKDKVLEHNKMYEEGKSSYNLELNAFADLKPREKLRALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSZ6</accession>
    <name>T1GSZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSZ6"/>
    <dbReference type="STRING" id="36166.T1GSZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006812-RA">
        <property type="protein sequence ID" value="MESCA006812-PA"/>
        <property type="gene ID" value="MESCA006812"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1397800_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="5">
        <location>
            <begin position="20"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006812-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="22123" checksum="7DA1B255C265C106" modified="2013-10-16" version="1">MSSGMKALNIRNVGTKVTHMQWNNKIDLLAIATEKSEVVIQRLNFQKLFSFPPPTCHDDPHKTMEITCISWHVSEPLLTVGYDSGTIFVLDVEKQAPQHCCCVKQRPVAIRCTQIEDNATIFEDEYRPKYEKVEIPFENFLPSFDKLCTVDQGRQSTQRIESYQCLSESISPIFLVVSCEDSISLITLGGLSNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSZ7</accession>
    <name>T1GSZ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSZ7"/>
    <dbReference type="STRING" id="36166.T1GSZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006813-RA">
        <property type="protein sequence ID" value="MESCA006813-PA"/>
        <property type="gene ID" value="MESCA006813"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_315747_0_0_1"/>
    <dbReference type="OMA" id="NDINSHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039005">
        <property type="entry name" value="CSPG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051561">
        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739:SF12">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 4-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16184">
        <property type="entry name" value="Cadherin_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51854">
        <property type="entry name" value="CSPG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="4">
        <location>
            <begin position="77"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="4">
        <location>
            <begin position="208"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="858"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="858"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="869"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006813-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="925" mass="105000" checksum="E43DB3282649A878" modified="2013-10-16" version="1">MQYSQEKMGYSSCKVFNLIDLSTDKIKYVHNGAENSDDHATIDLQIYGDTSHVPHNFLGKHRFLLHANITAVNDPPILQIPQNKILRLIEGIPKNINNDLLTIFDPDSPPHVLIYTVLPTNDPIASYGRFELNGKYTLTFSQADVNAGKVTYIFNNTMADSTSYQIQLQVSDGIETSEAVKNEISFFVNDGLFNTSDQILEIYPKPVVVTEVHNENLHVFPLTKKQVLRDHLQYMCSDESREIKYNITISPSLGRLLYEDYESGISKEISEFTQEDISEGRIFYEHTAVMHQFRINDSFYFDVIGDKAERLLDQKFNIEISVSSGGLLRFLPVSKVEVEEGDSAPIKLDFSKILEYLNSRAGISNPELYIESIQTPSFGKVELVDDITHTTRFHTSDFATGKVIYSHDHSDTLEDTILMSVFLVQGRIFLCNLTIPVSITPVNDQPFVLMTQTPHMTVWSHNWVFNEDISSRNAQEIIIYSNQFTQADINNDRIMYVHYGPPQSTTFYFNVSDGQYKPAYEIFNIKVAPITIIPHEDFVPISVQQGATSSVLRTSNLGFRTNAQLSRVQYNISNSPLHGIMLLDHQPTLRFTQQQLENGQITYMQTNMNKSNDTFQLVAYIPDTVYGTSMDVKVVIEPVLNKTNPKFVIYNPPKQGSIRKIIRSSGDNEAHTENIRSITVFTYKELKSGVIFYVTNSKPKLGQTDSFEYNLLIKSVQPAQSLVKIEYRIEDDMNEPTVMMSSPNLNFNYIAIIFLLIGIIVLIFVVLLMLKLRNVMRKKNLKQKDHPPSLPCPPDLTSSVGPIHNYASSEIDSMPVTAASTPIPGFSNIPHCKVIPVENFKNVDYSEYLGDMMGVEDDEEDDMDDPGETQQMVPNSNRYNNTYLDQDGWSSSCDMGNDFGYASVQQNLQTPNSNNPLLRKNQYWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT44</accession>
    <name>T1GT44_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT44"/>
    <dbReference type="STRING" id="36166.T1GT44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006864-RA">
        <property type="protein sequence ID" value="MESCA006864-PA"/>
        <property type="gene ID" value="MESCA006864"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017703_0_1_1"/>
    <dbReference type="OMA" id="FYCLVER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002997">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF72">
        <property type="entry name" value="HEPARAN SULFATE 3-O SULFOTRANSFERASE-B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="112"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="326"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006864-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="354" mass="41130" checksum="E7E01838A17FD2F4" modified="2013-10-16" version="1">MKMFVRPSCFSNRFLIVVLIFCISFLYFAYNFHWCLLLSIQRTWLKLNSSNNNNTILQCSSTTTANPPEAENATEIMSIKVIPFLLNANRSDLPSKYRFLQQQGVRPVRNLPDALIIGVKKGGTRALLEFIRLHPDVRASGCEVHYFDRHYSKGLQWYRHRMPYTIEGQLTMEKTPSYFVTKGVPHRVHKMNPKTKLLIVVRDPVTRVISDYTQAASKKSDMKKFEQLAFTNTTKAIDTTWGPLKIGVYARHLEKWLRYFPLSQLLVISGERLISDPAFEIGRVQDFLGLKRVVTEKHFYFNSTKGFPCLLKSEARPRPHCLGKTKGRNHPYIEPNAIERLRAFYRPYNTNFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU61</accession>
    <name>T1GU61_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02143542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007269-RA">
        <property type="protein sequence ID" value="MESCA007269-PA"/>
        <property type="gene ID" value="MESCA007269"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624826_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007269-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8723" checksum="74C6E8345684CF7A" modified="2013-10-16" version="1">MRFSEEHEPKRLDVFEGGVTLTSVSLVVCGLDRDLLTFPSLPEEIASSTLQDYESPLFLKLWMEAPESGLLMCGFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUA1</accession>
    <name>T1GUA1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007311-RA">
        <property type="protein sequence ID" value="MESCA007311-PA"/>
        <property type="gene ID" value="MESCA007311"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039880_0_0_1"/>
    <dbReference type="OMA" id="PRFNWTS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF149">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C X DOMAIN CONTAINING, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="79"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007311-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="315" mass="37162" checksum="BD2CDAF8A6610E0B" modified="2013-10-16" version="1">MHRWAVNNGKDKILTQVSPKFNNGWFVSQLKYNYKESAKVTKETRCYAYWVTYLNVEGQILKTQCLRAEPTWMNEMKSFIGNLRMRDLFIPGTHDSGSYRPGFKPRWYESPITKYALTQDEDIRGQLYHGVRYLDIRPAYHKKSKTNFYVNHGITKQRPLMEVIEHVRDFVRDTNEIVIFGVKEFPVGFGKDLTIHRLLVNLLKEEFGELIIDPSLSWSVKLNDIWNRKQNIFLAYDKQEMIDEFPNVLIQSVQQRWGDVRNWKDLEKYLRGLAVYDKDRTSDRPVADMAEMTANVFGVVLDKWGGLRRMAMKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUB4</accession>
    <name>T1GUB4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUB4"/>
    <dbReference type="STRING" id="36166.T1GUB4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007324-RA">
        <property type="protein sequence ID" value="MESCA007324-PA"/>
        <property type="gene ID" value="MESCA007324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888324_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7127" checksum="6F55DC9239EDD3AD" modified="2013-10-16" version="1">MSSSPKSMNSNSVRMHNYEELNVIGTGAYGIVYRARDLRNPGKVVALKKVRVTLMKMAFLCRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUM5</accession>
    <name>T1GUM5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007442-RA">
        <property type="protein sequence ID" value="MESCA007442-PA"/>
        <property type="gene ID" value="MESCA007442"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856327_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007442-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7482" checksum="7C4058CC8E24C09C" modified="2013-10-16" version="1">MASLFYFPKPCDWGHYPPPVKRSENFQVVSKYVLPFIIASIVLGIWGMNICVRMINGIVMESKMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUX8</accession>
    <name>T1GUX8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02117842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUX8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007550-RA">
        <property type="protein sequence ID" value="MESCA007550-PA"/>
        <property type="gene ID" value="MESCA007550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128551_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012813651" description="Farnesoic acid O-methyl transferase domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="2">
        <location>
            <begin position="52"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="180" mass="21384" checksum="C6D000EF52C21EBE" modified="2013-10-16" version="1">MKFIILLFIFVEIYGFNKNINISKLSGCRELRLDGTGNNLLSFPITSFINNHANDNEKLRMKFYVIGYDFYFYFKYSETQNHQFRACIEGMKRSTCKIQSYINEKKDLQVVNCENLTHDFYYTELTLIIAKNGVIQFYKKNENIPFLFADASFGFPLTHLSFDRYENETVDTRLFFDCPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV31</accession>
    <name>T1GV31_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02145735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007612-RA">
        <property type="protein sequence ID" value="MESCA007612-PA"/>
        <property type="gene ID" value="MESCA007612"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778759_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007612-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7999" checksum="7BEC13AE23CBEAE6" modified="2013-10-16" version="1">MSFSNYTSLGCATFSFHFTLRFSINFCPLSLWENFWKYLGDFRLDTSNQEVLSLGFLVKLEISILEIGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVQ0</accession>
    <name>T1GVQ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007864-RA">
        <property type="protein sequence ID" value="MESCA007864-PA"/>
        <property type="gene ID" value="MESCA007864"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976056_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007864-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14827" checksum="6D5F6CAEBD956F1D" modified="2013-10-16" version="1">PRGKPFDPKTYYNPNYSSERRTFTSEGASGRRGYGSVGRNADEERSYYEPYRPASQTYDDQLRWKPCYCVSSSQDRRRRSGGDTVKATSSVIQVVDDKLDGPFKRSQRRFREEKSNLSRSWFKKTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW18</accession>
    <name>T1GW18_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">STPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW18"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007986-RA">
        <property type="protein sequence ID" value="MESCA007986-PA"/>
        <property type="gene ID" value="MESCA007986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1541861_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048998">
        <property type="entry name" value="STPR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21107">
        <property type="entry name" value="STPRs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STPR" evidence="2">
        <location>
            <begin position="101"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="20603" checksum="7F3ABE606DAC0E2A" modified="2013-10-16" version="1">MSAKGKSVAHILEPQVICGEMEHQGRGRPPKPQEFLTHSGVEGLHMSRDFDDDSKKLDLRFEIASQQQYLSYLESTVNDIPELKAQKEVDIELVRSKIRKLKLRLSQRNRLATENQEQREHRLEKLRNNQRKRLANETPEKRQKRLQAMRTYALQRASKLRVKKKKKHCNHFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX00</accession>
    <name>T1GX00_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenylate kinase active site lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX00"/>
    <dbReference type="STRING" id="36166.T1GX00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008337-RA">
        <property type="protein sequence ID" value="MESCA008337-PA"/>
        <property type="gene ID" value="MESCA008337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_0_1_1"/>
    <dbReference type="OMA" id="FECKSIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22472" checksum="F9D00E1FB5A89FC7" modified="2013-10-16" version="1">MLRTIRRANDAFVFFQVNVPIIWVLGGPGCGKGTQCAKIVEKYNFTHLSSGDLLRAEVASGSEKGKELAAIMKEGKLVSNEAVLQLLDCAIKKAAASSKGFLIDGYPREKDQGAAFEKQISPADLILFFDCADQTLVDRILARAAASKEVRADDNEETLKTRIATLEAIPMIFWLNIQRRPLSSTLKDQLMKSLPMLLQLLMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX29</accession>
    <name>T1GX29_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RAWUL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX29"/>
    <dbReference type="STRING" id="36166.T1GX29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008367-RA">
        <property type="protein sequence ID" value="MESCA008367-PA"/>
        <property type="gene ID" value="MESCA008367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925252_0_0_1"/>
    <dbReference type="OMA" id="INKPMEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAWUL" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7194" checksum="891E2FB79C115C10" modified="2013-10-16" version="1">MRLTLDLGNDLPQELLNFCIYVAPQPSHLVILNGNQTLHQVNEKFWKINKPMEMFYSFKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXP6</accession>
    <name>T1GXP6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7 9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7 9">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02107402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXP6"/>
    <dbReference type="STRING" id="36166.T1GXP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008599-RA">
        <property type="protein sequence ID" value="MESCA008599-PA"/>
        <property type="gene ID" value="MESCA008599"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1212412_0_0_1"/>
    <dbReference type="OMA" id="FWSSADK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="11">
        <location>
            <begin position="84"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008599-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="229" mass="26105" checksum="50F67F9E2A990C83" modified="2013-10-16" version="1">TSSSKSEEPKPKKGRTTKKIKESEEVAPIKDVASNENIEDDFQEPSPKKSKTKSPKAEKMNSTVTNYSDIDFSLTKEFNLKISSWNVAGLRSWLNKDGLKYLEHEKPDILCLQEIKCEKEKLPDEARIPGYHPYWLCNKGGYAGVAIYSKIMPINVEYGIGDKEQDENGRIITAEYEKFFLVCVYVPNSGRGLVTLDKRLRWNTLFKDFISNLNSKKPVIIAGDMNIWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY06</accession>
    <name>T1GY06_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY06"/>
    <dbReference type="STRING" id="36166.T1GY06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008721-RA">
        <property type="protein sequence ID" value="MESCA008721-PA"/>
        <property type="gene ID" value="MESCA008721"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922333_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008721-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="15126" checksum="595D059C1FDF6D72" modified="2013-10-16" version="1">EIIETPDGPFNCPVCGRKLQTIAALKRHTASKHSSRLSKHDCSKCNKSFKTKWSLSTHNSRFHRDARLKEEIKLHIVELAKLVELLFCMIIFCIATVSEDLLPRIIHLLISALTQIYEVLHDLDVMIVHIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYJ5</accession>
    <name>T1GYJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYJ5"/>
    <dbReference type="STRING" id="36166.T1GYJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008924-RA">
        <property type="protein sequence ID" value="MESCA008924-PA"/>
        <property type="gene ID" value="MESCA008924"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_2_3_1"/>
    <dbReference type="OMA" id="PEDWPGF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF398">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="234"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008924-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="430" mass="49557" checksum="81D366365EEA7DAE" modified="2013-10-16" version="1">MKSTARIVIIGAGASGISAATKLVSFGFKNIIILEAQDRIGGRIYTIPFGDNFIDMGAQWCHGEKDNVVYELVKDKGYLDTTGNIYEDYEVIRSNGEKISDKTSEKLKEIIDIYLLDKKKDLPGFGGSLASYLRDKFLKTLKYPEYSDIDRKVAEEFLKFYHKYEVSMECCDSLFDVSGEGHLDYWNCEGDIGLNWKDKGFTMILRSLLNSNGTDFGILNKKIFLRKEVTDILWDPTGAIQGLGVGTVNKAFIHFEERWWDKDWCGFCILWREDDLKDIRESEFFWLEDVFGFYTVKYQPNVLSSWITGTSAKYVESLPKQKQMEGFIYLLERFLKRRIPKPIDFIATSWYTNKFIKMSYSHRSMETDHLKTGASELAEPILSPNGRPLLQFAGEATHNHYYSTVHGAVESGWREADRLNQYYSDTKSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU3</accession>
    <name>T1GYU3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009030-RA">
        <property type="protein sequence ID" value="MESCA009030-PA"/>
        <property type="gene ID" value="MESCA009030"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760687_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009030-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8437" checksum="85DC3CAB8976FEA3" modified="2013-10-16" version="1">MGKANLIYMEDSPVGIKVILNRLSYRKESEIRNTLEKCYRLVYMFLKFYQIYWTFSDILTNIDNVTEEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ70</accession>
    <name>T1GZ70_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02130992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ70"/>
    <dbReference type="STRING" id="36166.T1GZ70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009168-RA">
        <property type="protein sequence ID" value="MESCA009168-PA"/>
        <property type="gene ID" value="MESCA009168"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631252_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="17295" checksum="0263210D46D200DC" modified="2013-10-16" version="1">YTQLQPQRLLLQNQQQPQNPAQNQQQNPQQTQASCKYANDPTTTPTGNNNQSISSPQDLSTRDISPKLSPSSVVENVARSLNKSVVGGGGSGGNVTSTSLSSSLNNNHNTNIPIPMHSPTGDSDSESDSGNEAGSSQNSGNGKKGNPPQIYPWMKRVHIGQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZG2</accession>
    <name>T1GZG2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02196478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZG2"/>
    <dbReference type="STRING" id="36166.T1GZG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009262-RA">
        <property type="protein sequence ID" value="MESCA009262-PA"/>
        <property type="gene ID" value="MESCA009262"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2694943_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009262-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8454" checksum="D5E680FDA7686491" modified="2013-10-16" version="1">MGTVIGACVPPDTDNNTLYYCNHAYSNSNQPRVYCCIKCPVCSKFVLPDDIECHLVMCLTKPRLSYNGEYFFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZU6</accession>
    <name>T1GZU6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009403-RA">
        <property type="protein sequence ID" value="MESCA009403-PA"/>
        <property type="gene ID" value="MESCA009403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963401_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="7104" checksum="D3F4A3EF9CC09C99" modified="2013-10-16" version="1">MRSKEELELYPMKEACSASWEEIINKRQAGKSEHDLIDVQFYRCKKWGIMLPRGKLREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H022</accession>
    <name>T1H022_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02375159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H022"/>
    <dbReference type="STRING" id="36166.T1H022"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009486-RA">
        <property type="protein sequence ID" value="MESCA009486-PA"/>
        <property type="gene ID" value="MESCA009486"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943422_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009486-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14189" checksum="5F701C643CBE9052" modified="2013-10-16" version="1">MNATTDTLSGFESAGYAAYSTALSVTIAIGCSLLILNVLIFAGVYYQRDKTRLEVKSLQKQYQQRTGVPLHQQVQFDPIKHAHYHLGHAQSASAVIVDVENHDQGSIILSGDIKSPHICGNTMQITTNNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H055</accession>
    <name>T1H055_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02372878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H055"/>
    <dbReference type="STRING" id="36166.T1H055"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009523-RA">
        <property type="protein sequence ID" value="MESCA009523-PA"/>
        <property type="gene ID" value="MESCA009523"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2364864_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043653">
        <property type="term" value="P:mitochondrial fragmentation involved in apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016543">
        <property type="entry name" value="Fis1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247:SF0">
        <property type="entry name" value="MITOCHONDRIAL FISSION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009523-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="13093" checksum="622E99A0CC83A11F" modified="2013-10-16" version="1">MSAEMEDFLKDTVNPKDLEKFEKNYRRELEANNVSHSTQFEYAFCLVRSQYARDIQTGILLLEDLMTRHVEEREITYIIWPLETQESNNTQMQLNTVKPSFKSNPTTHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0G8</accession>
    <name>T1H0G8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02091395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0G8"/>
    <dbReference type="STRING" id="36166.T1H0G8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009646-RA">
        <property type="protein sequence ID" value="MESCA009646-PA"/>
        <property type="gene ID" value="MESCA009646"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006586_4_2_1"/>
    <dbReference type="OMA" id="HYLLMAP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147032" description="Carboxylic ester hydrolase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="28"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009646-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="22994" checksum="6692D00CC1711849" modified="2013-10-16" version="1">MHPSGSLVTVIFFFQACGALASNRNLIIKLNHGGSLEGIELKTETGRSFLGIPYAKAPVGELRFKSPVPYPGWEGQRSATEDGSSCTQAFALLADEGNEDCLFLNVYTPPLENITNSGLSVMVWYHGGAWFAGSNNHLFYGPEYILDHDIILITVNYRLGALGFLSSETLDCPGNFGLKDQVEALRWVKEHISSFGGNPNDVTIFGESAGGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0P3</accession>
    <name>T1H0P3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATPase F1/V1/A1 complex alpha/beta subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0P3"/>
    <dbReference type="STRING" id="36166.T1H0P3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009726-RA">
        <property type="protein sequence ID" value="MESCA009726-PA"/>
        <property type="gene ID" value="MESCA009726"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444393_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009726-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="20355" checksum="33D571DE33642369" modified="2013-10-16" version="1">QPLPLLPVNSAPKADLKETGHVLNIGNGIACVYDLKNTQADEMEEFSPILKVMMDLNLEPDNAGVVVFGNDKMVKQSDIVKSTVQTFRNVTLILGNAIKGPINIKDRFRVGIKASGVIPSASNLESMQTGIRNCIVSTSILVKNCSTVAQVVKRLTDVGAMDYIIIISATAIIIIGSILWMRHGRILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0W3</accession>
    <name>T1H0W3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0W3"/>
    <dbReference type="STRING" id="36166.T1H0W3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009808-RA">
        <property type="protein sequence ID" value="MESCA009808-PA"/>
        <property type="gene ID" value="MESCA009808"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009808-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12967" checksum="BAF0441738D5FEEB" modified="2013-10-16" version="1">MAPADINPSQREVTPEQINSWCENNKMFTYLETSSKTATNVTEAFSLAVKQWRKLEKQTERELRDQGDTIDLTKGVTLPQNRICCSHLSEIVDLIPQQLQLPELELIIHSTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Y0</accession>
    <name>T1H0Y0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit C N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Y0"/>
    <dbReference type="STRING" id="36166.T1H0Y0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009827-RA">
        <property type="protein sequence ID" value="MESCA009827-PA"/>
        <property type="gene ID" value="MESCA009827"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161244_0_0_1"/>
    <dbReference type="OMA" id="YMAGHEF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit C N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009827-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="13199" checksum="8B915FC81973834E" modified="2013-10-16" version="1">MVTAKPKELLAQGLLPQRQHKRSEKQEKFEKQRLMPFHMPINLELLECVYLVSAMILEIPYIAAHEFDARRRMISKTFYQKLRSSERQSLVEMRKLACSNFIINMKMNTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Z1</accession>
    <name>T1H0Z1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02040113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Z1"/>
    <dbReference type="STRING" id="36166.T1H0Z1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009838-RA">
        <property type="protein sequence ID" value="MESCA009838-PA"/>
        <property type="gene ID" value="MESCA009838"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_785940_0_0_1"/>
    <dbReference type="OMA" id="CCKSMSS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009838-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="353" mass="40755" checksum="4897F65F236CC891" modified="2013-10-16" version="1">MDATQLYLDGNNFKEISSHAFIGRKRLNILYLNHSHIDTIKNRTFYGLLELEILELSHNNLDKLYGSEFHGLDNLKELYLQYNKITTIDNLSFANLHQLKILRLDHNLIVDFAIWSFLPSYMINLQISGNPWRCDCVFNYRMSEYLARHEFITDRDFLKCDTSICSSTSSSSNNVALPTTTEQLSPSNDPMTKLVLQPHVIQASNEILIGMTCGFLFIVFIFIILFIFRQEVRIYCHSRYNIRLGLCCKSMSSMSSTEDNDFNKSERDKPFDAFISFSSMDEPFIMEQVLPALEDYRICVYHRDADCPDSIVHAIDTSRRTILVLSQISLKRNGVDLNSNQPIIKCYVIGGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1G8</accession>
    <name>T1H1G8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02091234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1G8"/>
    <dbReference type="STRING" id="36166.T1H1G8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010029-RA">
        <property type="protein sequence ID" value="MESCA010029-PA"/>
        <property type="gene ID" value="MESCA010029"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932815_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF2">
        <property type="entry name" value="PROTEIN RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="28"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010029-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14455" checksum="5B7E4F73CCEF6018" modified="2013-10-16" version="1">MTTKTQYDPSSGSIPAESLLIYRPDRRHEIWRFITYMVLHANWFHLGFNIVVQLIIGLPLEMVHGSIRIAAIYIAGVFAGSLGTSVVEPLTSLVGASGGVYALLAAHFANVLLNFNYMKYGILKLASILIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1U3</accession>
    <name>T1H1U3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1U3"/>
    <dbReference type="STRING" id="36166.T1H1U3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010163-RA">
        <property type="protein sequence ID" value="MESCA010163-PA"/>
        <property type="gene ID" value="MESCA010163"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443374_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10504" checksum="574B2E294110F4D8" modified="2013-10-16" version="1">MNNWFSPNAEFLKCCVRDFKTHFYECDFRIGPNGGDNHMLKEFLEGSYMCHQGRCYQRPLEKALKKTCVAEVHEIFKDHGDPVIKAFDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1V1</accession>
    <name>T1H1V1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1V1"/>
    <dbReference type="STRING" id="36166.T1H1V1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010171-RA">
        <property type="protein sequence ID" value="MESCA010171-PA"/>
        <property type="gene ID" value="MESCA010171"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1890968_0_0_1"/>
    <dbReference type="OMA" id="IAIMDFW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002426">
        <property type="entry name" value="ADH_Ceratitis-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF8">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01169">
        <property type="entry name" value="CERATITISADH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01167">
        <property type="entry name" value="INSADHFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010171-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="14535" checksum="DC7E4E542B1FE295" modified="2013-10-16" version="1">MTDLKGKNIVFVAGLGGIGLASCTSFLKRKVANLVILDVVEKPEVIHNLRSNYTETNIDFMAVDVSKKSNIELCLNEIIKKIQYIDILVNGAGIVADQNVELAIAVNLTGLINTTLLAIPLMDKSKNGRGGIIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H207</accession>
    <name>T1H207_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TATA-binding protein interacting (TIP20) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAND family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H207"/>
    <dbReference type="STRING" id="36166.T1H207"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010236-RA">
        <property type="protein sequence ID" value="MESCA010236-PA"/>
        <property type="gene ID" value="MESCA010236"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007157_1_1_1"/>
    <dbReference type="OMA" id="AYIPHFQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010265">
        <property type="term" value="P:SCF complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039852">
        <property type="entry name" value="CAND1/CAND2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013932">
        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12696">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08623">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25782">
        <property type="entry name" value="TPR_CAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="4">
        <location>
            <begin position="702"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010236-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="783" mass="87604" checksum="E08634ABFA83A5D2" modified="2013-10-16" version="1">MDTEDEEYGESEEYSDDDDMSWKVRRAAAKCLEAVISTRHEMIDEFFRTLSPALIMRFKEREENVKSDIFHAYIALLNTMRHTSDDSIPNDPDSMDQVSGTMSLLQNQVPAIVKAVEPLMTEKSIRTRQDCFALLKELLIILPGALGAHIPQLMSGIVCSLSDKNATSNMKIDALGFVCSLLQSHNPEVFHPHIQTLVDLIVKAVFDPFYKIATEALVVLQQLVKVIRPLEAERIQHNFDIALLDQIYSATLQKLKSTDVDQEVKERAIACMGQIISNMGDILKNELIVCLPIFLDRLKNEVTRLSAVKALIMVASSPLKIDLKPILQEIIPILGSFLRKNHRALRIQSLQLLNTIVENYSDSYNPQLLQGAIQEINPLLSDSDLHIAQLSLILLTCAARYQPQALMGGIHEQFLPAVLILLKSPLLQGAALQCTLDLFQALVRAKLPNLSYNQILKKICCRLTVECPNEAVPLAMKLLQELRKATNNDAQLVFCLLSIGEIGRHFDLSSIDQLPQTIIECFNSVSEDVKGAASLALGAVAVGNLSTYLPLILREIESQQKKQYLLLHSLKEVISSLSTTPNGLNQLLPSVPIIWDQLFKNCECQEEGSRNIIAECLGKLVLVDPEQLLPRLRAALKSQSALMRTTVVAAIKFTISDQPQPIDTLLKQCIGEFLMALRDPEPSVRRVSLVAFNSAVHNKPSLVRELLPSMLPSLYEETKVNESLIREVEMGPFKHTVDDGLDIRKAAFECMYTLLEQGLDRVDIMLFLDHVQAGLRDHYDIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H334</accession>
    <name>T1H334_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Elongation Factor G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115697">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115698">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H334"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010646-RA">
        <property type="protein sequence ID" value="MESCA010646-PA"/>
        <property type="gene ID" value="MESCA010646"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801294_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation Factor G" evidence="4">
        <location>
            <begin position="64"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010646-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="15667" checksum="39E1F8415C5E1829" modified="2013-10-16" version="1">EPLADEYREIGEVCAGGVAVCSGLKNTITGDLLTSSTSSLKSAESKIAKESEDESNILSLKPYIPDAVYFCSIEPPSLSFQTPLEIALKQLQREDPSLRVVFDETTGQTVLGGMGELHMEVXXXXXXXXXXXXXXXXXXXTWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H382</accession>
    <name>T1H382_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H382"/>
    <dbReference type="STRING" id="36166.T1H382"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010701-RA">
        <property type="protein sequence ID" value="MESCA010701-PA"/>
        <property type="gene ID" value="MESCA010701"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533973_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010701-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9064" checksum="1E54A830F999DB0E" modified="2013-10-16" version="1">MFVDRGNQEIFYKPSQISSGDQTAVILCSSGSTGLPKAVTISHNVMKTIGMLFSETCEDVFLSFSSLFWISGLNSMINTAMTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H392</accession>
    <name>T1H392_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H392"/>
    <dbReference type="STRING" id="36166.T1H392"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010711-RA">
        <property type="protein sequence ID" value="MESCA010711-PA"/>
        <property type="gene ID" value="MESCA010711"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204762_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="46" mass="5183" checksum="27ADC079D91DCFF9" modified="2013-10-16" version="1">MNDESGQEKGEVDKKIVHVYPLIKHSDMGEEMKTEAIELSMTACET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3A6</accession>
    <name>T1H3A6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3A6"/>
    <dbReference type="STRING" id="36166.T1H3A6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010726-RA">
        <property type="protein sequence ID" value="MESCA010726-PA"/>
        <property type="gene ID" value="MESCA010726"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1557039_0_0_1"/>
    <dbReference type="OMA" id="SAMNNIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF1">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010726-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="19559" checksum="91BFB91C982522F1" modified="2013-10-16" version="1">MTTEDVVLEFKEKGNESFKNGKWEEAIEYYTKAIINGEHHKQLAVLYKNRAAAYLKINDFDSALADSTATLFRRSQAYESIGKYEEAYKDAVDLLKSDPNNKTVQPILERLHKITQQRATENAQTSTKVNKMINLAFDLTQPIDKRKSAMNNIVVLAREDVGADLLVKEGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3E8</accession>
    <name>T1H3E8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pro-resilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3E8"/>
    <dbReference type="STRING" id="36166.T1H3E8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010773-RA">
        <property type="protein sequence ID" value="MESCA010773-PA"/>
        <property type="gene ID" value="MESCA010773"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_892232_0_0_1"/>
    <dbReference type="OMA" id="FGHEENR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF85">
        <property type="entry name" value="PRO-RESILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577849" description="Pro-resilin" evidence="4">
        <location>
            <begin position="18"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010773-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="32467" checksum="57F24B865A42B785" modified="2013-10-16" version="1">MMKLVCLLLIVSTIVLARPEPPPQTYLPPSTNYGEPEPGQRGSSGRGSGSGFGFGGSGGGGTGSGRPSSSYGPPSGSSGLGDGNTPSDSYGTPEFGGGQSGSGGGFSSPSSEYGPPNRQSDFGSGSNNRQKPSSTYGTPEFGGGRNGDRNGIKKPSSTYGAPGQGRNGGNRFGGQSGSGGSSNDEPAKYEFSYEVEDAPSGLSFGHSEMRDGDFTTGQYNVLLPDGRKQIVEYEADQGGYRPQIRYEGEANTPPPDRVDLVGEHHLKVMVRPKVVVEMAFQEEVDLPPPPPPPPPPPPPPPPPPPWTKWIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3I4</accession>
    <name>T1H3I4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein yellow</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major royal jelly protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3I4"/>
    <dbReference type="STRING" id="36166.T1H3I4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010812-RA">
        <property type="protein sequence ID" value="MESCA010812-PA"/>
        <property type="gene ID" value="MESCA010812"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192441_0_0_1"/>
    <dbReference type="OMA" id="LLECYFI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="MRJP/yellow-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009:SF12">
        <property type="entry name" value="LD43175P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PROTEIN YELLOW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010812-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26542" checksum="4196A90F00C83CD1" modified="2013-10-16" version="1">TTTKSPALIPYPSWQSHNLQDEEPEIVSPFRMRADKCGRLWVLDTRVDNLLGEKKKYGNAQLLIYDLHNDFLQRRYVFPDSQVKSESFFANIAVEDGDCDNTFAYAGDLGAPGLVVYSWKLQDSWRVNHHYFHPDPLAGNFTINGTNFQWDDGIFGLAISKQQPDGFANLYFHPFSSTMEFSVSTKVLRNKTHAESSDNYDDFKILGSRGPNGESGASFLTSLLECYFIHYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H441</accession>
    <name>T1H441_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H441"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011042-RA">
        <property type="protein sequence ID" value="MESCA011042-PA"/>
        <property type="gene ID" value="MESCA011042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239662_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12104" checksum="C0C5BF9D89F00142" modified="2013-10-16" version="1">MAKESQPQTRYSYYGKSSRRPMDSDDITRFARAIDEQQQKTEKYGRQLLINLIEGSPSWICMYQYLLHNCEISNASFDEIFLSSKIDSIVLVYISASSPGGRELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1H477</accession>
    <name>T1H477_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02136195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H477"/>
    <dbReference type="STRING" id="36166.T1H477"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011080-RA">
        <property type="protein sequence ID" value="MESCA011080-PA"/>
        <property type="gene ID" value="MESCA011080"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_5_2_1"/>
    <dbReference type="OMA" id="WENAYLL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000008">
        <property type="entry name" value="Cathepsin D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577869" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="60"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="91"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011080-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="22709" checksum="C8FD942EB687E105" modified="2013-10-16" version="1">MIKILVLSLLSIALVGATSLPRVAFKKTLLPSKTSPRSYRYVNGQGVGIVPQNNSQDVYYYGEITIGTPPQPFVVLFDSGSANLFVPSIDCDSANYPCSQHHKYNASASSTYKDLNTPYNMADWATGYLAQDVVNVGGIDIKYQVFAQATQMGSSFDSDIYDGLLGMAFVPIAVDNIVPPFINMIDQNLLEEPIFSVWMDRNAEASNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H488</accession>
    <name>T1H488_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyl transferase 64 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196698">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H488"/>
    <dbReference type="STRING" id="36166.T1H488"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011092-RA">
        <property type="protein sequence ID" value="MESCA011092-PA"/>
        <property type="gene ID" value="MESCA011092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013906_2_0_1"/>
    <dbReference type="OMA" id="GRERWHH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF5">
        <property type="entry name" value="EXOSTOSIN GLYCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase 64" evidence="6">
        <location>
            <begin position="112"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="322" mass="37463" checksum="DCAE6D3B7E8BF829" modified="2013-10-16" version="1">MANNYILPFEDVIDWNLAIIRIHENNFGSILKILEGVSSQKIKELKEQGSWLFKKYFKDLKTITMTMFDILNDRVFPHQAKSLKQWNTRTYSTPSAQNPLFLPMQAPKSQGFTAVILTYDRIESLFTLIQKVSVVPSLQKILVIWNNQKKSPPHPSMFPKISKPLKVIQTKANKLSNRFYPYDEIETEAILTIDDDIVMLTADELDFGYEVWREFPDRIVGFPSRIHIWDNTTQRWKYESEWTNQISMVLTGAAFHHKFWSYMYTNSMPGGIKEWVDEHMNCEDIAMNFLVAILQTNLRLKSLPERSSSAPNAQTPKCCPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4B3</accession>
    <name>T1H4B3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4B3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011121-RA">
        <property type="protein sequence ID" value="MESCA011121-PA"/>
        <property type="gene ID" value="MESCA011121"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834076_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011121-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7693" checksum="38D7721CC73BD11A" modified="2013-10-16" version="1">MHFKGQYQFFKSAKKITMEKSKLNLLRLFETPKQEIYVTTEASSRVVSRALTNIKNVAQNEVEIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4C9</accession>
    <name>T1H4C9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4C9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011138-RA">
        <property type="protein sequence ID" value="MESCA011138-PA"/>
        <property type="gene ID" value="MESCA011138"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2090799_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011138-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13426" checksum="4BE2B0D603012EF8" modified="2013-10-16" version="1">GTEFIIFTDPNPIIFATQHPDNTSPRQLRLLGIISQFSTDIPYKSSLAFKFLAIQNSDKLPQCDTSCDRIRPWVPKTLRKRVIENIYSIAHSERKAILKLIKDIVFLPEEEEKVAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4S2</accession>
    <name>T1H4S2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4S2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011290-RA">
        <property type="protein sequence ID" value="MESCA011290-PA"/>
        <property type="gene ID" value="MESCA011290"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161231_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011290-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12546" checksum="A6ACF5A260C17DFF" modified="2013-10-16" version="1">MDIKNIDTSYCSNSVDIHINIIVANIINVNTSDNTNITNSDDTNIGYIKNITNNIGFSTNNVHVNYQILETNFPNPIHVPDHHVLVNHWEDANNFYSMFPMVNKLESSAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H520</accession>
    <name>T1H520_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H520"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011394-RA">
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        <property type="gene ID" value="MESCA011394"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1566808_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011394-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="19149" checksum="BC8B88D0A90DA9BD" modified="2013-10-16" version="1">KVESEKKSSPVKTEASLTDSQKSKSSEKTEEDSKEVIDESKSKSPVKDEKAEKSPVKESKSPNSKSPVKKGKSPQKDSKSPSKSSKSPSKDETEDDVIEIKDDETEPKNTRTMWITAAMSTPYFSNTSRDPVDRKIGDSQILRNTEDADEEVKDLFYDNGFIAYSFEESCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H534</accession>
    <name>T1H534_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <taxon>Megaselia</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H534"/>
    <dbReference type="STRING" id="36166.T1H534"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011408-RA">
        <property type="protein sequence ID" value="MESCA011408-PA"/>
        <property type="gene ID" value="MESCA011408"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984108_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011408-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14461" checksum="5F4808011A2BDCF9" modified="2013-10-16" version="1">MQKYSLEYHHATCLIFMQHSLSTSPKNDHFCVFMSRSSETVKSFTSALGLPTVSGAYGQEGDLRQWRDLTEDKQKYLLQVMPPVDMIPQVVRSIVKYQNITNAAILYDDTIDTYILSIFLKLGKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H566</accession>
    <name>T1H566_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
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            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1H566"/>
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        <property type="gene ID" value="MESCA011441"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415765_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011441-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10175" checksum="34AC3B051B07C289" modified="2013-10-16" version="1">MRILNSGNIIVSTLPAISTEGLTKDDVQVLIDNVHKQMSAVYKDTSAEVLNKVSLQHKTKEALKSFKKTIPNTTAVEKLSVYNNFKDTVIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5D2</accession>
    <name>T1H5D2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5D2"/>
    <dbReference type="STRING" id="36166.T1H5D2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011509-RA">
        <property type="protein sequence ID" value="MESCA011509-PA"/>
        <property type="gene ID" value="MESCA011509"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393317_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588733" evidence="2">
        <location>
            <begin position="22"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011509-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="21308" checksum="4D67F450C1027FD7" modified="2013-10-16" version="1">MALKLKTIFLICAICALAVYGEDTTTSTTPKTTSSSSSSSTSSSSSSTTTTTTSTSTTTTTSTTEAPISTTTKAPPLPAPKVGEWNSTCIKLKFAAQLSFSYNTTDNTTKKSAYNFPTEIITVGDFECNFNETESIILQWVPSNETKVVNSAKFTFYKDEKSYRLGMIHIKLEPKSIFPDANNDSVQLIYANNKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5E9</accession>
    <name>T1H5E9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1H5E9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011526-RA">
        <property type="protein sequence ID" value="MESCA011526-PA"/>
        <property type="gene ID" value="MESCA011526"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127866_0_0_1"/>
    <dbReference type="OMA" id="WTANFRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF154">
        <property type="entry name" value="PROTON-COUPLED AMINO ACID TRANSPORTER-LIKE PROTEIN PATHETIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011526-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="17988" checksum="8B029C1D2B00DBE6" modified="2013-10-16" version="1">MSWVTLIYMLLGFMGYLRFRDKAEKSITLNLPVHEIPVQIVKIAIALAAYCTFGLQFFVCLEIVWNPLKDKCPPYCSCNSCSVPTIAPFMVLIGAFCFSILGLIMPVFVEIVTDWETGFGAYNWKNVLIVLLGVFVLIFGSESAITDILKQLGGSGATDGTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6J9</accession>
    <name>T1H6J9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6J9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012332-RA">
        <property type="protein sequence ID" value="MESCA012332-PA"/>
        <property type="gene ID" value="MESCA012332"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067276_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012332-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13191" checksum="6DF70A8684410A97" modified="2013-10-16" version="1">TKAAGFVIFRRISEKVEYLLLKASYGSFHWTPPKGHVDPGGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6S4</accession>
    <name>T1H6S4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="3"/>
    <dbReference type="STRING" id="36166.T1H6S4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012407-RA">
        <property type="protein sequence ID" value="MESCA012407-PA"/>
        <property type="gene ID" value="MESCA012407"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000001">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028356">
        <property type="entry name" value="UDPglc_DH_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374">
        <property type="entry name" value="UDP-GLUCOSE DEHYDROGENASE/UDP-MANNAC DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase N-terminal" evidence="9">
        <location>
            <begin position="130"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase dimerisation" evidence="8">
        <location>
            <begin position="204"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="293" mass="32134" checksum="3A393977E1AD9386" modified="2013-10-16" version="1">SNVCCIGAGYVGGPTCSVIALKCPNIKVTVVDKSADRIAQWNSEKLPIYEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKVQFLIMQILKANQKHNVRYDILSNPEFLAEGTAINDLLYADRILIGGEDTPEGHQAVDNLAWIYQHWIPKENIITTNTWSSELSKLAANAFLAQRISSINSLSAVCESTGADVSEVAKAIGLDSRIGSKFLQASVGFGGSCFQKDILNLVYICEGLNLPEVAAYWQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H735</accession>
    <name>T1H735_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H735"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012517-RA">
        <property type="protein sequence ID" value="MESCA012517-PA"/>
        <property type="gene ID" value="MESCA012517"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1202442_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012517-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="231" mass="24676" checksum="E87CC906860ABF9E" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGGGAPAAAAAAYGQQLGCWYSPTTAAVTPATAASVQNQYLQGIQGYXXXXXXXXXXXXXXMGVQIPGTWQNATTPTAAPQIAQSVGGAIPQTAGVVAYPMQSFQVSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7K5</accession>
    <name>T1H7K5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7K5"/>
    <dbReference type="STRING" id="36166.T1H7K5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012742-RA">
        <property type="protein sequence ID" value="MESCA012742-PA"/>
        <property type="gene ID" value="MESCA012742"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086803_0_1_1"/>
    <dbReference type="OMA" id="NRIMARY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14829">
        <property type="entry name" value="Zeta-COP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000013">
        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="8"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012742-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14679" checksum="917B3C42358E07B1" modified="2013-10-16" version="1">MDGYLNIIKGMCILDNDGRRIMAKYYDKNVLPTVKEQKTFEKNLFNKTHRANSEIIMLDGLTCVYKSNVDLFFYVMGSTQENELILLSVLNCLYDSISLILKKFVEKKSVMENLEIIMLAFDEICDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA26</accession>
    <name>T1GA26_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Magnesium transporter NIPA2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIPA family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA26"/>
    <dbReference type="STRING" id="36166.T1GA26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000070-RA">
        <property type="protein sequence ID" value="MESCA000070-PA"/>
        <property type="gene ID" value="MESCA000070"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645098_0_0_1"/>
    <dbReference type="OMA" id="HNSFYIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF92">
        <property type="entry name" value="SPICHTHYIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000070-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8486" checksum="D8E82C98E3536B93" modified="2013-10-16" version="1">MSTQALGSTEKSFADAYNTKEFYVGLSLAVSSCFFIGSSFIIKKLGLLRLSRHGEVRAAAGGYGYLRDWIWWAGLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAG3</accession>
    <name>T1GAG3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAG3"/>
    <dbReference type="STRING" id="36166.T1GAG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000219-RA">
        <property type="protein sequence ID" value="MESCA000219-PA"/>
        <property type="gene ID" value="MESCA000219"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998247_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577209" evidence="1">
        <location>
            <begin position="26"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000219-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="13948" checksum="0E5022BE1C6A4D6D" modified="2013-10-16" version="1">IQNQKPFHFQLFGVALASAAPVAGGEGKYTHGSYDNGKYNPKTRNSGRYVHDVMPYMHIRIYHHIPNPYDGGYGPYFGIQNPYVHDGRGTYVPPPVSTRAPQYIDIPVPAIKLEYGFPDISPKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBP5</accession>
    <name>T1GBP5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000682-RA">
        <property type="protein sequence ID" value="MESCA000682-PA"/>
        <property type="gene ID" value="MESCA000682"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1435960_0_0_1"/>
    <dbReference type="OMA" id="MERKCAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052417">
        <property type="entry name" value="Dachshund_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12577">
        <property type="entry name" value="DACHSHUND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12577:SF6">
        <property type="entry name" value="DACHSHUND, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000682-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="22018" checksum="F5180831CF06321B" modified="2013-10-16" version="1">MDVIREREVKDSLERQLVDERKLRILYQKRYRRERKARIRLQQQMDGKRKTNSHITDRERDIIKGSSENCKNNNNNNQEIDCKPSDKTSEKSQSSERHRRSESPSFQRESSERAVERDRESTADRESASSTLTQNPDGSSTPGNNNGKGWNYPGIDLMATGAFWQNYSETLAQEIEMERKCANKLGTEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBW9</accession>
    <name>T1GBW9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02086267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBW9"/>
    <dbReference type="STRING" id="36166.T1GBW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000758-RA">
        <property type="protein sequence ID" value="MESCA000758-PA"/>
        <property type="gene ID" value="MESCA000758"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943428_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000758-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14563" checksum="B0D3ECBED3CD5384" modified="2013-10-16" version="1">MKVEYMTIQVGNTMINSKDIIKYIAVMIYNRLIFEFHVESAIEKASKIQSMLDRMLPIIGGPKYQKRILLSRFLSSAHFSCICNMVESGESGRLAALKIISGYRTISGGAVYVLAGRKAQPLVSFEDYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC88</accession>
    <name>T1GC88_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC88"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000892-RA">
        <property type="protein sequence ID" value="MESCA000892-PA"/>
        <property type="gene ID" value="MESCA000892"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173838_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12320" checksum="CF3BE6C09A9CC753" modified="2013-10-16" version="1">MKSANISKAAIVAKKFRANRIAWPTVGHVRHILPICAEHSSVMKQFDVKIVFLIMYGRSLIDIQRVTQNLVYPLISQIFGLRCTTRGHYPIPVTCLVGDTPAENKLISMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCE1</accession>
    <name>T1GCE1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dipeptidase E</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S51 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02114883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02114884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCE1"/>
    <dbReference type="STRING" id="36166.T1GCE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000952-RA">
        <property type="protein sequence ID" value="MESCA000952-PA"/>
        <property type="gene ID" value="MESCA000952"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044162_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03146">
        <property type="entry name" value="GAT1_Peptidase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005320">
        <property type="entry name" value="Peptidase_S51"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003642">
        <property type="entry name" value="PRK05282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20842:SF0">
        <property type="entry name" value="ALPHA-ASPARTYL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20842">
        <property type="entry name" value="PROTEASE S51 ALPHA-ASPARTYL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03575">
        <property type="entry name" value="Peptidase_S51"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000952-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="29772" checksum="147754A2BB3E02C4" modified="2013-10-16" version="1">DQIETVLKPKDVKTVLFIPYALKNHDNYTKTVSDALTKWGYKVEGIHTHSDPVEAVKKSEAIFIGGGNTFVLLKTLYEKKLIETIRSRVLEEHIPYIGSSAGTNVATRSIHTTNDMPVAYPPTFDALKLVPFNINPHYLETDPSTKHKGETRDERIGEKDVKTVLFVPYALKNYDNYTKTVSDALTKWGYKVEGIHTHSDPVEAVKKSEAIFIGGGNTFVLLKTLYEKNLIETIRARVLGDHIPYIGSSAGTNVATKSIHTTNDMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCI8</accession>
    <name>T1GCI8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02123061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCI8"/>
    <dbReference type="STRING" id="36166.T1GCI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001002-RA">
        <property type="protein sequence ID" value="MESCA001002-PA"/>
        <property type="gene ID" value="MESCA001002"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530048_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF5">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001002-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="10086" checksum="F4BF9CBEF3BBD8EC" modified="2013-10-16" version="1">MSPTFEFYSLKQGIDWQQFVRNKLNGWEEMNLDWAKNFTGDLKIVFYDDLVRDVEGTLRDILHFINYKIDELLHACMHEGDKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCX5</accession>
    <name>T1GCX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001148-RA">
        <property type="protein sequence ID" value="MESCA001148-PA"/>
        <property type="gene ID" value="MESCA001148"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530031_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="39"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9747" checksum="3BF019AE0BC2B2A0" modified="2013-10-16" version="1">MEFERYPGAMSFYISGEEKDCNINNGWTKKFTRTIPAATPENITLTFISPTSVQISWQTTADIKSIEKYLVQYKPANDRWFSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDB1</accession>
    <name>T1GDB1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001294-RA">
        <property type="protein sequence ID" value="MESCA001294-PA"/>
        <property type="gene ID" value="MESCA001294"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511218_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001294-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="533" mass="62188" checksum="8FBEF6D540BD14F5" modified="2013-10-16" version="1">MEERNFETKDLGENPSTNDIIKVIEDIIKKLYKDEVPGTSGTQGHATDDNKKTKQIEDYHDNYQRYRRSRSPSRENRKRKHYRRSSSVERSHQKYRERSSSRSSHRRSPHRRRRSSSRDSYDYKYSKRHRFLRSSSLSRCYDCDYNFGWKYKHFIENERERDEFKEMMEFYKQLRAKESCKRDVFKNRLETIIEEPVVCQKVHREIKPKHQNLKSESSSPDKVIIVDDDSDEDTIPHRRDFVQKTSKPVNDQDKIHGILKTKKSVRFLANLFEEPVEKKEDVTKKSKNSSSPINISVSDRKRKFELDNNIEVPPKLKAKMAARRKSFESVRSLFENACDFDNLSMISNISTASTIKNENIPRKAVDPTPSTSKNCTKNIATSTPILNKNIPERKASFEKEQKPIINPPKPSSKVVPKESPVQKSTQVKLKPQEASTPSTSKEAMTKIKVQETSTPSTSKEAMSKLKTQEPSSVEDIAVLEINFDSGNPPRNFQVFLQNIEDILGIAPHSKAFYNKNTLYLWLKRSYGTWIMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDD7</accession>
    <name>T1GDD7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM76 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001322-RA">
        <property type="protein sequence ID" value="MESCA001322-PA"/>
        <property type="gene ID" value="MESCA001322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880602_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032017">
        <property type="entry name" value="FAM76"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46176">
        <property type="entry name" value="LD21662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46176:SF1">
        <property type="entry name" value="LD21662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16046">
        <property type="entry name" value="FAM76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="14787" checksum="EAC2386490FEDA4E" modified="2013-10-16" version="1">IASLLKEVANLKKSNKKNGIGSSKADKEKDKDSSSAKDSATSKPEIKQSNSGNSSGSGNGGMSNFTAMMATPMVDPNSSDHVVAMTQLKEKLVSLGKKLTQKDKELLDKDKLITELKSKNFENEIELRNKLKDTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDV5</accession>
    <name>T1GDV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02139783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GDV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001506-RA">
        <property type="protein sequence ID" value="MESCA001506-PA"/>
        <property type="gene ID" value="MESCA001506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_6_1_1"/>
    <dbReference type="OMA" id="QCFWGAE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000004">
        <property type="entry name" value="Peptide methionine sulfoxide reductase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF13">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="6">
        <location>
            <begin position="42"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="28724" checksum="8B39A2E472152067" modified="2013-10-16" version="1">MIQADENIQFAFDKIKDKVFIQNEQTEFNVTPTHKLDKNFETITFGMGCFWGAESLFGATKGVLRTTVGYMGGTTDQPKYTKLGDHVEVLEIDYDPEVITYKELLDIFWNNHEYGLSTKVKRQYQSFIFYHTEEQKRLSEISKAEEEIKRNPEIITTEITEAVKFYPAEDYHQKYRLQEHKEFMNSLGINNTEILHSSYVGAKMNGFLVGVGDFLEEVDTLGLTPTQKEYAKHYFEKNQGRGLYCXK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEM4</accession>
    <name>T1GEM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001792-RA">
        <property type="protein sequence ID" value="MESCA001792-PA"/>
        <property type="gene ID" value="MESCA001792"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1322252_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001792-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="23303" checksum="7FFEC33DBE461A3A" modified="2013-10-16" version="1">MMRSIKLQLLEPFRSTGSTSSWYSLTFVFLKSCLHRRSLLRLYRHILDNPSSSTLVLLSDVTSEPKSIYATHKGDLETCTMSNGKQVEILMKDVIFSKNFCMNLMSIKRLQANGLEVHFENSNCSTYILAGASPILGLVVEQQKSPLTAHHQILLFVFAYSSSHNFAENDRSSQRAVIVDVVRSNNCCLLFKSKSGSAPVHKSDPVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GES4</accession>
    <name>T1GES4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Larval cuticle protein LCP-17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GES4"/>
    <dbReference type="STRING" id="36166.T1GES4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001845-RA">
        <property type="protein sequence ID" value="MESCA001845-PA"/>
        <property type="gene ID" value="MESCA001845"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_6_1_1"/>
    <dbReference type="OMA" id="DGTHRHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF233">
        <property type="entry name" value="CUTICULAR PROTEIN 47EB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588233" description="Larval cuticle protein LCP-17" evidence="4">
        <location>
            <begin position="17"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001845-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13368" checksum="4115B9B1DD414FAD" modified="2013-10-16" version="1">MIKLIVVLAFIGFLAAGPTQTTTKPSSIAILETSRKHDEDGSWNFSFKSSDGHTREEKSVVTDPGSKDEEKKFSISGSYKYIDANGKEVEVHYTADENGFVPIGTGIPESISNAAKGIDVYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFI8</accession>
    <name>T1GFI8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02012002">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFI8"/>
    <dbReference type="STRING" id="36166.T1GFI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002130-RA">
        <property type="protein sequence ID" value="MESCA002130-PA"/>
        <property type="gene ID" value="MESCA002130"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032403_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002130-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="14409" checksum="78DD55EEEE5D6352" modified="2013-10-16" version="1">NNKQHLNFILGRHRRAELENVEILLICGYPRRKMNPSTLLTGFSRINLLQIEYGNLTEFQGNFPQMNNLETVNITWTNLSELKPSIFSKVVNLKYLDLRYNSLNLFERPLELSKTFQKMHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG10</accession>
    <name>T1GG10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002314-RA">
        <property type="protein sequence ID" value="MESCA002314-PA"/>
        <property type="gene ID" value="MESCA002314"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485916_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002314-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10212" checksum="6B27959D6A06E27A" modified="2013-10-16" version="1">MSVSLIRLLRSKQLSRKIKVRIYHQLILSVLLYGTEKNGGLGTIRYVIYNHLDVDLNKTFALGRICRNDGRFCRRFVRNKVKVLIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGG5</accession>
    <name>T1GGG5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGG5"/>
    <dbReference type="STRING" id="36166.T1GGG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002485-RA">
        <property type="protein sequence ID" value="MESCA002485-PA"/>
        <property type="gene ID" value="MESCA002485"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_607315_0_0_1"/>
    <dbReference type="OMA" id="QHCSAHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006616">
        <property type="entry name" value="DM9_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649">
        <property type="entry name" value="AGAP009604-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649:SF10">
        <property type="entry name" value="IP19903P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11901">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00696">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002485-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="465" mass="50983" checksum="BDEB7FCF01A796D2" modified="2013-10-16" version="1">IYHHNWVHSSIESPLPELAVVGGHDSDGTTKDNPSNRSAYTCCEGYEIFKNHYEVLCGTGYSWMQCENNIIPGNAVSCGRTCEGEQVYIGRGHFDRSLTIGKLFRGDDCLYIPYGGRERRVHRYEVLIKDSFVVPAQTYETSYTTTYTKYEPGTTYVTPANTDGYYKPSAATTYVTPASTGGYCKPEVDLLISPAQTGAEAPPLYEKTPSYAGLYPPVPPTSSTDAFLKNEATTPYPPPPPVGFIKPEANYPAPTLTPSPILTANTASAPATEMPMPIPPPPYTLKEEVAVVHKKPPAFGIQVLPPPYQKEIEDTNNVIWLKWKSDIVPRDAVYAGVDTDLSTLYVGRCFHNGDLLPAKVNISKACAYVSFNGHEVLKEDYELLTGSGFEWIPSHDGYVPQDAVIGGRTAGGETLYIGRGQHMKSITPGKIQPSENCLFIPFGGGEVKLQNYDVLVKRDGKILNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH07</accession>
    <name>T1GH07_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002689-RA">
        <property type="protein sequence ID" value="MESCA002689-PA"/>
        <property type="gene ID" value="MESCA002689"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3126722_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577377" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002689-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="50" mass="5819" checksum="1B0D86D071D03775" modified="2013-10-16" version="1">MSLWLLLLVIIAACMIPDYTIKMCREALKMPKMELFPKPKGFGKRQSTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH31</accession>
    <name>T1GH31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189636">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH31"/>
    <dbReference type="STRING" id="36166.T1GH31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002715-RA">
        <property type="protein sequence ID" value="MESCA002715-PA"/>
        <property type="gene ID" value="MESCA002715"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012979_0_0_1"/>
    <dbReference type="OMA" id="ANCESYQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021910">
        <property type="entry name" value="NGX6/PGAP6/MYMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319">
        <property type="entry name" value="FIVE-SPAN TRANSMEMBRANE PROTEIN M83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12036">
        <property type="entry name" value="DUF3522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="581"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="384"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002715-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="635" mass="72220" checksum="C49D352B42B3B199" modified="2013-10-16" version="1">MRAKGSRLAFSSALASERNWTNPNNERIEQQGLAFTKSCITKLKSELFVNTIDPIFLTNSKEVRNTLNDQKGNQNNIYSILIPEKVEKATIRILFLKPINDTPLLKVFIKSKSTPTNLNYKYMFKLDPRNYTQSDFNISVISNAWHFITVEASTKNSSFEVQYSINISFTQSAENIIENEFGYRMVRFQDIDFYETKRVNQMDFFLFDVEIFNSNGSSTDFFNLTSGINYGFRFHVYDCQDIGGTLTSLISLVNSDENIVFNKASDRMAEGPDIIFEQSSIKPEKLVLKSPDRISEQNLTVIKIAIICMNLEEPGIPELPGKCRYGNTFHNASAIVKTGEWFLTIQVFCIIDQKGKSLTDLLVESYSNENEVDECNGTFIISISSMPCGPNQCGSFGSCYHYMSGGFVYTTCVCRNGYRGWDCSDDSDVHSLGLILFSILLLTLSNLLFIPSVYLAIKRQYFTEAFIYFCSMFFSSFYHACDSGEDVYSFCLVRLSVLQFCDFYCGLLSIWVTLIAMANLRIKYVSLAHMIGAIGLAFGTEYDKQSLTVFLAPALIGICIISICWIYKCKKTKALYPSKKYLTIWLPIGAVLVMFGLICYAFLQTKQNYHIIHSIWHVVMAISILCLLPNRKIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHF9</accession>
    <name>T1GHF9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHF9"/>
    <dbReference type="STRING" id="36166.T1GHF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002856-RA">
        <property type="protein sequence ID" value="MESCA002856-PA"/>
        <property type="gene ID" value="MESCA002856"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142458_0_0_1"/>
    <dbReference type="OMA" id="TDTTFCF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032222">
        <property type="term" value="P:regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23591">
        <property type="entry name" value="TFP_LU_ECD_Crim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031424">
        <property type="entry name" value="QVR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050975">
        <property type="entry name" value="Sleep_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562">
        <property type="entry name" value="ATILLA, ISOFORM B-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562:SF28">
        <property type="entry name" value="PROTEIN QUIVER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17064">
        <property type="entry name" value="QVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002856-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="17224" checksum="E864F834BBD2C4FA" modified="2013-10-16" version="1">MYEDEENLSRAINEAIWCYRCTSATPGCGETFNWKGIGILGEHCPEPDDVCVKVTERRGATEVITRDCLSALSFRTDIPADKYEGCRKAAHDPKLAHYVNHTIKEHDVKRTFYNEIEFCFCFLDHRCNSSKLHMISVPLLILVAAILRISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHN0</accession>
    <name>T1GHN0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02112321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHN0"/>
    <dbReference type="STRING" id="36166.T1GHN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002938-RA">
        <property type="protein sequence ID" value="MESCA002938-PA"/>
        <property type="gene ID" value="MESCA002938"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_7_2_1"/>
    <dbReference type="OMA" id="CWHPELA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.830">
        <property type="entry name" value="Ile-tRNA synthetase CP2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="9">
        <location>
            <begin position="1"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="10">
        <location>
            <begin position="553"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002938-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="728" mass="82577" checksum="BFFBD8223D35A6A3" modified="2013-10-16" version="1">MNGKKINYIPGWDCHGLPIELKALAEGTKASPLEIRTKCAAFAKEALERQKSGFKEWGVAADWELETSTYQTNRPGYVSNQLQLFYEFYQKGLVFRDLKPVFWSPSSKSALAEAELEYDNNHESPSVFLQLKLENVPDAFAVIWTTTPWTLPSNQAVCYNPSLEYALVYLDGNTSKKYLIATSLITSFSETTNMTIDISETISGKDLQGFRYQHPVDSKILPFIAASHVEDSKGTGLVHTAPAHGPDDFLISLEHKIPVLSLVDEEGKYTSKAPDFLVGKQVLEEGNKLVMEKASEQWFMNTESLKTRATEEIEKIKIYPRVNEEANRKMLMTQVQKRPYWCISRQRSWGVPIPVFYKKDTKEIVLNREIVDCIANLVSKEGSIDFWWSKNPSEIISPDILGRLEISPENLVKGNDIFDIWFDSGTSWSSVLGENKIADLYLEGYDQFTGWFQSSLLTSVAHRNCAPYKSLFVHGFTVDDKGLKMSKSLGNVISPSEIIKKFGTDSLRWWVAAHGTQHMSITVSNKLLQQSAESVSKIRATLRYLNGVLGEGDRLYCGMDEDLAQIKSVLKECYLILNKAIWPVTPFLVEESWSYFDSSAPFYKNKSCVENDWENKEAEQIVTEALNLKRKINQMESTNSWLLHCSVNCTSEQLQYLSKLQPKLSEFVNNSELCEILQVGSVKLEESMESSIELKISETPLCPRCRRFQCHQGGVCERCLIVMKSKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIH0</accession>
    <name>T1GIH0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIH0"/>
    <dbReference type="STRING" id="36166.T1GIH0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003249-RA">
        <property type="protein sequence ID" value="MESCA003249-PA"/>
        <property type="gene ID" value="MESCA003249"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="OMA" id="ICKMATC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003249-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="24733" checksum="D0048682E64B5053" modified="2013-10-16" version="1">MKVLKKASIVTNQKDTAHTRAERNILEAVKHPFIVELVYAFQTGGKLYLILDEIILALGHLHSLGIIYRDLKPENILLDAQGHVKLTDFGLCKEHIQEGIVTHTFCGTIEYMAPEILTRSGHGKAVDWWSLGALMYDMLTGVPPFTAENRKKTIETILKAKLNLPAYLTPEARDLIRRLMKRQVPQRLGAALKIQTLYALIHSSNTSTGTMCWPDICLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIR4</accession>
    <name>T1GIR4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIR4"/>
    <dbReference type="STRING" id="36166.T1GIR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003349-RA">
        <property type="protein sequence ID" value="MESCA003349-PA"/>
        <property type="gene ID" value="MESCA003349"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2489751_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042508">
        <property type="entry name" value="FBXW5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20995">
        <property type="entry name" value="F-BOX/WD REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20995:SF17">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003349-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10047" checksum="8811400B5D3038ED" modified="2013-10-16" version="1">MLMETLKEIYLIAIIFCSGAEDMHAYLWDRYYGMCLAKYKHMDVVNSVAFNPKDSEMLVTTGDDFKIKVWRSKNRAKEYGIEAAPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIW6</accession>
    <name>T1GIW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02043791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIW6"/>
    <dbReference type="STRING" id="36166.T1GIW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003402-RA">
        <property type="protein sequence ID" value="MESCA003402-PA"/>
        <property type="gene ID" value="MESCA003402"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_669556_0_0_1"/>
    <dbReference type="OMA" id="FEDSTCI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003402-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="411" mass="46388" checksum="7C8475E1AC17AF08" modified="2013-10-16" version="1">MLVAKNIFISILGGKVQLVGSSPNSFQTDEGTLFSVISGIDPDTIKSNIFLFEDSTCINSRFTFIAINGIYAGTYFFVRTFLLKDVEIELPVIQEKRYIKIRSKLYSSLYNSLFKSLMPTFFYILFYWSFGGVFNRRIALVFGSQIDGELDSLIEIVSNIRLILYVWILSSQILTNMNLINSFFTMLLTEEIQFPIEKSIQLSDGATLVEVIGLDNLPIAKKLASRDLFNLSNESFSGRRQQVFSLSSPGNHPHTWNNLSAQCLVIINSYVDELKNSLTKISNLKNPIPGVRLCADSATLNAQKLLIRQYNQSCSIRRMMIDEPVSSQPQSTQQENILETIKKFIEQSVKNTMYAIPGLLYMFGEPDGAKTKYLINNSQEIVLVTQGLAGICDLPSLKINMELSKLNLEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKT9</accession>
    <name>T1GKT9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02027525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKT9"/>
    <dbReference type="STRING" id="36166.T1GKT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004119-RA">
        <property type="protein sequence ID" value="MESCA004119-PA"/>
        <property type="gene ID" value="MESCA004119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058697_0_1_1"/>
    <dbReference type="OMA" id="MHPRDEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053225">
        <property type="entry name" value="Acyl-CoA_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="GCN5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20958:SF10">
        <property type="entry name" value="GH05617P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20958">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="144"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="272" mass="31540" checksum="4B0616E8EF2364D4" modified="2013-10-16" version="1">MSYEYSLAEIPEHHYPKLRELFLEDWPSNYISFSVTEHSARHDSKSSQIYSVGGNIHDGLVHIDKIGNILHFCEFGNNLEKLKKALEDISFKRFEFIYFKSPKLIPLIPHLEIGEHRYVQETHGFFFLEKAKAFNLNIQERKDVFLKPLTLEDADIVNDLWPPKFPGSINYVEYLIKTDYSMGAFCKTTGNLVAWILRYPIGCLGILQVKREYYRRGLGKLVVTAISKKLGEEGLDCSVTINVKNEPSTKLFKSVGYEKPYDIFLYKNLNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKX8</accession>
    <name>T1GKX8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02118584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02118585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKX8"/>
    <dbReference type="STRING" id="36166.T1GKX8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004161-RA">
        <property type="protein sequence ID" value="MESCA004161-PA"/>
        <property type="gene ID" value="MESCA004161"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_547015_0_0_1"/>
    <dbReference type="OMA" id="CDIANTT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000033">
        <property type="entry name" value="teneurin-a isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF69">
        <property type="entry name" value="TENEURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Teneurin 1-4-like FN-plug" evidence="7">
        <location>
            <begin position="2"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Teneurin NHL" evidence="8">
        <location>
            <begin position="123"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004161-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="53405" checksum="C3FC4324968D933A" modified="2013-10-16" version="1">MRLTGETIPSTLTHVHVGVEIEGSLHVKTYEADPNLTHTFAWNKRNVYRQKVYGVTIARISVGYQHSTCKEPVWNTQTAKLQGYDVDISDIGGWGLDIHHHYNFHEGILQKGDGSTLHMKEYPRTVKVVMGTGLQRALNCPDHSLASGPDGSLYVGDFNLVRRITPDGKVFTILQLSATQVSYQYYLAVSPADGRLYISDPERHQILRLLSLDKVKDPSINSEPIVGSGQRCIPGDESNCGDGGPAIKARLSHPKGLAIAADRTMYIADGTNIRAVDPKGYIHTLIGHHGHHNHWSPAPCSGTLMANQAQLQWPTGLALSPLDGSLHFIDDRLVLRLTSDMKIRVVAGTPLHCNANSDKNKTSENVLGTVLAMAFSPFGDLYIADSDSRRVNSIRVVNTDGHMRYFAGKQENSGSMPCDCGALNGGGNHSTTYPTTVNPSRNNGSPGGTTPSLMSMITSTITGTGSGAGGSSTASNTNIGGCICAGNGVSLGTSTTSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL13</accession>
    <name>T1GL13_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004198-RA">
        <property type="protein sequence ID" value="MESCA004198-PA"/>
        <property type="gene ID" value="MESCA004198"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239665_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004198-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11941" checksum="0354B825E478B932" modified="2013-10-16" version="1">MQSQKLRSDHNPRRITMSLCALSHKVEILSPTLCPLSGECATRYTSTGSTISWNDTQRRYTRQSTRTRNGRDKSSNLEHKDHVDTRSRINVAPPTTTPVIAKYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLE9</accession>
    <name>T1GLE9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02198168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLE9"/>
    <dbReference type="STRING" id="36166.T1GLE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004350-RA">
        <property type="protein sequence ID" value="MESCA004350-PA"/>
        <property type="gene ID" value="MESCA004350"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006586_13_2_1"/>
    <dbReference type="OMA" id="RNACKNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147027" description="Carboxylic ester hydrolase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="19"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="76"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004350-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="468" mass="53307" checksum="7084525A28A379AE" modified="2013-10-16" version="1">MKYLLFGAVFLPLVNCYKTEIKISNGKIRGLEKSNYISFESIPYAEPPIGLNRFGPPKPYTQNWTDYFNATSSPVDHSLPVMVYIHGGAFSWSSGGINRPDWIMDHGKMILVTFNYRLGSLGFLTTEDDVIAGNMALKDQQLALKWIRTNIEAFGGNTEQIMLTGWSAGGASVQFHLLSSKSRQYFTSAVSFSGSVLTPIRTPSPRETAKKLARILNCPVTEDSKQIKECLISLPADKIVECHKHFLKIFYNPFVVFGPITENHSDHAFITESVTNLMTKPTNIPWITSYTADDGVYASAEFMRLNENGRDYMSYLNENWEDIAPYLFFYHGLNKDEQNKESKRLYKKYFGNGGISPFIWCSFECFCVCYIFNYPSPFGYGNLLSGRTDINFGCGHGDEVLLLFKTKIRDSYPLSMEDRCIQKKLLDMLYEFASNRLPVYDGKPLPSNTPFQCEIQYFKVPNYTNELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLG7</accession>
    <name>T1GLG7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLG7"/>
    <dbReference type="STRING" id="36166.T1GLG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004369-RA">
        <property type="protein sequence ID" value="MESCA004369-PA"/>
        <property type="gene ID" value="MESCA004369"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083127_0_0_1"/>
    <dbReference type="OMA" id="PENSEIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053027">
        <property type="entry name" value="AGGF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23106">
        <property type="entry name" value="ANGIOGENIC FACTOR WITH G PATCH AND FHA DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23106:SF24">
        <property type="entry name" value="ANGIOGENIC FACTOR WITH G PATCH AND FHA DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="90"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="3">
        <location>
            <begin position="261"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004369-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="32646" checksum="447A33A56F8534D7" modified="2013-10-16" version="1">MHRNGTLSKNDRLERPKKKKKLEPKTGENGDLSEEGEICSSDEEATEVEENGIFEDIAKKYPPSLRLVVQETNLEKIQIGTLFIVTYKGGSLGREGNHDVIIPDINVSKYHLKFQYDEGSSMYKCIDLGSKNGTILNGKRMSSSMQESEEYELVHGSVVQLGFTKLLCHIHSGSSTCGLCEPGLLMKEKKVENENTTSATLTHKEQLKKLQKKYGLEDDKYRENQGDSTYKDRAATRRVKVGSSHVKEKTQSSSLNTAIESNNKGFQMLSKLGWNKGDTLGKSSDGSWNLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLS8</accession>
    <name>T1GLS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA004492-RA">
        <property type="protein sequence ID" value="MESCA004492-PA"/>
        <property type="gene ID" value="MESCA004492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1148351_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052317">
        <property type="entry name" value="Viral_replicn-host_int_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44665">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44665:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="129"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="28302" checksum="A1848DF4C539615A" modified="2013-10-16" version="1">MELLNALLSQIFENFLKLLSWLFYLIYDIVVIGFSMAYEQSLFYYSIYVVRLKSWIKDIHQNSDKPNIWIKAKWKKFDARFPKTSKWAFWRNFYKKKPIEASSEFYKNGRLPSTAEEAMYSLLNCKGKDAYSILGVQADCSQEQIRKHYKKIAVLVHPDKNKQAGAEEAFKVLRRAFELVGEPESRKTYDRSLLEALNAEKAWSELHDLLSQLQTKISEAANTIRXXXXXXXXXXXXANTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLV8</accession>
    <name>T1GLV8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02167961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004522-RA">
        <property type="protein sequence ID" value="MESCA004522-PA"/>
        <property type="gene ID" value="MESCA004522"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134025_0_1_1"/>
    <dbReference type="OMA" id="YNTFSAP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685:SF2">
        <property type="entry name" value="825-OAK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685">
        <property type="entry name" value="825-OAK-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577501" evidence="1">
        <location>
            <begin position="18"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004522-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="13002" checksum="98F944971A6D67C5" modified="2013-10-16" version="1">MFKLTLAIFAVIACAAAKPGLVAPLAYTAPVVAAPGVVTATSSQFIARNHHGIVAPVVAPVAAKILAPAPLAYTAPIVKAPLAYTAPVVASAPIVKAAAPLAYTAPVAYSSPLVHSHLASPLTYSAPLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLY5</accession>
    <name>T1GLY5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02004158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLY5"/>
    <dbReference type="STRING" id="36166.T1GLY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004551-RA">
        <property type="protein sequence ID" value="MESCA004551-PA"/>
        <property type="gene ID" value="MESCA004551"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1351892_0_0_1"/>
    <dbReference type="OMA" id="LEFHESM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000010">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="16">
        <location>
            <begin position="38"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="14">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="14">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004551-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="22543" checksum="E429312C09E37537" modified="2013-10-16" version="1">RFSPFGRFKLNSNDNTEEDALNLSSAIWFAWGVLLNSGIGEGTPRSFSARVLGMVWAGFAMIIVASYTANLAAFLVLERPKTKLSGINDARLRNTMENLTCATVKGSAVDMYFRRQVELSNMYRTMEANNYETAEHAIQDVKNGKLMAFIWDSSRLEYEAAKDCELVTAGELFGRSGYGIGLQKASPWTDAVTLAILDFHESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN10</accession>
    <name>T1GN10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043457">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004949-RA">
        <property type="protein sequence ID" value="MESCA004949-PA"/>
        <property type="gene ID" value="MESCA004949"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515248_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004949-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9649" checksum="180BA4B639B3AE90" modified="2013-10-16" version="1">MERVFILRGLNYMISLAYGFLSIHSSSLHENLQTFGAASDCSLFFSSVSGLSTVLLRLHIDFLSNYHLQCKISHKLLTTYYDLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN28</accession>
    <name>T1GN28_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase subunit b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02005784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN28"/>
    <dbReference type="STRING" id="36166.T1GN28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004967-RA">
        <property type="protein sequence ID" value="MESCA004967-PA"/>
        <property type="gene ID" value="MESCA004967"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1333277_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0138">CF(0)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004967-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="206" mass="23765" checksum="9B7E44BF3C7B9D73" modified="2013-10-16" version="1">MLTRKLFSFTRNQSRSFFFKKKKQCECEKELDDCIKGVTSSDVPDIEVPYMRPVRFEAPKVRLGFLPDNWFTMLYDKTGVTGPYVLGAGILTYLFSKEIYVCDHEFYGGLSLLIAIIYVVKKLGPKLANYCDKEVDKIETAWKNEKKVLTDMLNKDIEMEKMSQFAAEGGLLMLDAKKETCNCNWKLNIEEGSTMLIKKSNIDWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN87</accession>
    <name>T1GN87_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005031-RA">
        <property type="protein sequence ID" value="MESCA005031-PA"/>
        <property type="gene ID" value="MESCA005031"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3052733_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005031-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="54" mass="6159" checksum="493C633C54098E81" modified="2013-10-16" version="1">MIRLLSSVDPTLTPELMENATVSDTPPTNWDTIQSLNLILKFQIQRNHLLLLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNI2</accession>
    <name>T1GNI2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005135-RA">
        <property type="protein sequence ID" value="MESCA005135-PA"/>
        <property type="gene ID" value="MESCA005135"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870177_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005135-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7426" checksum="D566573557557AD7" modified="2013-10-16" version="1">MKYLVNYLQAFVDCPGGQCLPIWGLSNILTTWLILSQRYIDQEFPENSVPFLNIFCYNNSFATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNR7</accession>
    <name>T1GNR7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02107396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNR7"/>
    <dbReference type="STRING" id="36166.T1GNR7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005226-RA">
        <property type="protein sequence ID" value="MESCA005226-PA"/>
        <property type="gene ID" value="MESCA005226"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1422996_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005226-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="22492" checksum="65A1D454B69C6A06" modified="2013-10-16" version="1">MNDFKYSLEYGNSTRYIEHFMKIQDKIKSDLKNSPFIKCLWENKNQDCSKIFKYFVTEDGICYTFNSFHPSDIYRKESVLKEFTFGEEASRQWKLDIGYEEYNLETEVYPRRAIGSGVNSGLNTYLIDFINQNDALCRFNLDGFKVILHMPNEVPRVSKDYFQVPLKQKILVSVSPTVVNTSEELKDYDEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP85</accession>
    <name>T1GP85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GP85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005406-RA">
        <property type="protein sequence ID" value="MESCA005406-PA"/>
        <property type="gene ID" value="MESCA005406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004193_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF466">
        <property type="entry name" value="RING CANAL KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="79"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1119"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1194"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1000"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1119"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1138"/>
            <end position="1147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1221" mass="136276" checksum="D4A9CF66F38A0147" modified="2013-10-16" version="1">MNNQNGNMQPQNPAAEGNIIDRGSCLLRYASQNSLDESSQKPMQRPSGREKSSGSYRNDQHTNRSFLVMNEMRKQNLLCDVTLVADGMECPCHKMVLASCSPYFYAMFTSFEESRQDRITLQNVDFQALQLLVEYVYTSLVEVTEENVQVLLSAANLLQITDVRDACSDYLQTQLDASNCLGIRAFADLHGVIELTNYADSYIEQHFHEVILFDEFLNLNNEQVINLVRNDRISVPNEETVYECVIAWLKYDPEERSKYISELLQHVRFPFLSKEYLAQKXXXXXXXXXXXXXXXXXXXXXXVDKEPLLEESIDSKXKALRYHLMPNETKSARTVPRKPAVRPKVLLVIGGQAPKAIRSVECYDLREEKWFQAAEMPNRRCRAGLSVLGDKVYAVGGFNGSLRVRTVDVYDPATDTWSSCNGMGARRSTLGVAVLNGCIYAVGGFDGSTGLNSAEVFNPSTQEWRFLANMSTRRSSVGVGVVNGLLYAVGGYDGAARQCLCSVEKYDPERNIWTPAADMGYRRSGAGVGVLSNILYAVGGHDGPLVRKSVEAYDPERNEWHSVAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDGMLYVVGGDDGTSNLASVEVYSPKTDSWRILPATMSIGRSYAGVNNSTAATGDDDNSQAEGTNDGGYLRPIDLPAAGAAAANRQPVYENIYESLEQYNNAVEDPVANAQDAVENAIAPAENIPQNQQNAQQQEAAEHEQVINYQVPNPIPQPVSAASTPNPTRSNFIYRNDFYDRSHITYDVPRSARMVRRNFQIDLNRPGSSRCSYGTCGRNPSFDEDPHQYYNVNYKNHSNIYEQINDDPIYRKAKVSNPNSRVMGKLNVIGHGVGRIERHLSSSCGNIDHYNLGGHDYAVLGHSHYGTVGHIRLNSSSTTTTTTTAQQKDSGVKTSLXSGVKTSTSFFSCISGDNAQSISSINRSSSETTTTTSSTTARCTGAIPKSKIASNQLASKMKAKPALPNRSQSPENCSTLNRISKNSLQWLLVNRWMPLWISQGSDCKIIDFNFMFSRNCGECEYENVVGPERPRVLPFSQDLVRYRRDRLFKPIKSTLNKLKETEKPESNYENVHVQFQNFEFGRSREDRPTLTRARSESPTFSQRLKRQQNHHVKFPDPFKNYELNTENNSFKPKALQAKIQKVEPSEKPVDPIEIVEEEGAVGGIDFVEAPEDQPSTETETNDKNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP88</accession>
    <name>T1GP88_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sodium-dependent nutrient amino acid transporter 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP88"/>
    <dbReference type="STRING" id="36166.T1GP88"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005409-RA">
        <property type="protein sequence ID" value="MESCA005409-PA"/>
        <property type="gene ID" value="MESCA005409"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639624_0_0_1"/>
    <dbReference type="OMA" id="SANETMH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="112"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="23"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005409-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18277" checksum="FC9AA40AFF420987" modified="2013-10-16" version="1">SLNSIIGHYIYSSFKSPLPWAKCREEWGPNCVNSDGTPSTFKFDAFMAIASNKSISSSELYFNNIVLQERDNIDDGIGVPNWPLVITLFLTWVIVLMAIIKGVRSSGKASYFLAIFPYIILIILLIRAVTLEGALEGILFFIKPQWDKLLSPQVSLFYLFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPW1</accession>
    <name>T1GPW1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPW1"/>
    <dbReference type="STRING" id="36166.T1GPW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005656-RA">
        <property type="protein sequence ID" value="MESCA005656-PA"/>
        <property type="gene ID" value="MESCA005656"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973018_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14208" checksum="609972DD303505D1" modified="2013-10-16" version="1">MDGSILPCFVLFCSPRVNRYSPKTPPTYNTFPVRGITISSGSYFCNQFMCNAEIDGLSQLSSGTYKCEVSEDAPEFKLIDQTANMTVGATCCFGGKSYAKRFVALNWREYKGHRGDSLRIGHRQTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPW4</accession>
    <name>T1GPW4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPW4"/>
    <dbReference type="STRING" id="36166.T1GPW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005659-RA">
        <property type="protein sequence ID" value="MESCA005659-PA"/>
        <property type="gene ID" value="MESCA005659"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568964_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF2">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="9">
        <location>
            <begin position="70"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="480" mass="53292" checksum="3E43BCE60A15A946" modified="2013-10-16" version="1">MIRRFCQINKILRAQPISRRLNLRCLASDGIMSYILKSIFPFLYPLDTTSRPQSYEPKSCDEMDGNFVEEVVCKITDINDNEMKALPFGEDNKILLIKENGEFSAIGDKCSHYGAPLQTGALGNGVTGDIEDFPGLDSIPCYKVKVEKNGEIRVRAKKSELEKNKRIKNMAKRCESDERTYVVVGGGPSGGVAVETLRQEGFTGRIVLICKENALPYDRIKISKALDTTVDKLQFRQASFYEEHGIEVLQGVEATKLNTSEKTVQCSNGYSIKYDKIYIATGSKAVKPPIKGADLGNIFTIRDVTDSNAISKIINKDMEVVCLGASFVALEAAAALVNKVKKVTLVGREEMPLKASLGPEIGQSVLHLFEQKKVNMLMKSGIEECIGGEDGNVKSVMLKDGSIIPCDLLIMGTGTRFYTEFLRNSDVAMNQNGSIDTDMYLMSNIPTCMLEVILQMHQYSRLPIKELQLDIINWPSTMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ53</accession>
    <name>T1GQ53_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Synapsin ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ53"/>
    <dbReference type="STRING" id="36166.T1GQ53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005754-RA">
        <property type="protein sequence ID" value="MESCA005754-PA"/>
        <property type="gene ID" value="MESCA005754"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1505153_0_0_1"/>
    <dbReference type="OMA" id="RMAIVCK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020898">
        <property type="entry name" value="Synapsin_ATP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841">
        <property type="entry name" value="SYNAPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841:SF17">
        <property type="entry name" value="SYNAPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02750">
        <property type="entry name" value="Synapsin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Synapsin ATP-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005754-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="19724" checksum="822B4693B94BF6AE" modified="2013-10-16" version="1">MLEQMPVSDKHKMWIEEISKMFGGLVLCEISFLVDRNGVEYLHSFKDSSFPLIGDSQEEDRKNIVDIVSSKMQVRKLICTPGAVMSRKNSVDNSQTTPLRQPPLGGPPPIPERISPAIGSIGRLSSRSSISDTPAPTLNLARRDSQSSTTTESAPTQPEDTEDTMKNLRKTFAGIFGDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQU0</accession>
    <name>T1GQU0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006005-RA">
        <property type="protein sequence ID" value="MESCA006005-PA"/>
        <property type="gene ID" value="MESCA006005"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2336007_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006005-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11250" checksum="BB9B383826149F85" modified="2013-10-16" version="1">MSNSSTSKDESFCDGNFAGLSDHQIYGRGLEWAKLIEKLSMRTKNESIENSIGLDEFEVLFKDQDQDNETDDDEAVEDNSDILKIIEQEEHNEDFNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR16</accession>
    <name>T1GR16_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR16"/>
    <dbReference type="STRING" id="36166.T1GR16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006087-RA">
        <property type="protein sequence ID" value="MESCA006087-PA"/>
        <property type="gene ID" value="MESCA006087"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_650984_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006087-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="422" mass="50546" checksum="F72D74C1CE00B5AF" modified="2013-10-16" version="1">MEPRNPEDESDLPPPASTNEPSESSDPAEECAPAKPKSRRRCSDLSNLEQWGWHKNKRYSTRKKSNQEKEVDTSVNGYLRKIFARHFEETFDTQSPFLLEKRNEENGMDAITEEDEQKADSTMKDFETEKKEDFEKFLKEARESHLDLILIIYLWIKYVSNYWNQELPEDIKKIYYEIYGFYLNHYDLVSWNMLSNEDFNTSYSTTLFFLEMDIERENATFQEHCREINAQWKMLFYHLKFNSGNSFVVCEEYDINMLRLFHLEYAWAKFENRLNNSSEGYVFSLPNFKDFKCSTLENVTNLRVELQRKIGLKNVKTLYENNKFEDLVEILKDSLIYSTTIKNSDDNVMKLQTQFEVLLESFWNVEKYEECLVWSERCLKYSVDNIVDLFPDTFRYKEWADCINFILVYIEELILKDGFDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR72</accession>
    <name>T1GR72_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006148-RA">
        <property type="protein sequence ID" value="MESCA006148-PA"/>
        <property type="gene ID" value="MESCA006148"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075828_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13304" checksum="89010B4BE14126F5" modified="2013-10-16" version="1">MAKLNITVNDNYVLPIINITKPTDASLNAKTIKADIFSYDYDYKDELDKYPDTQSRHSNLNISSSIYSEMPDESDAMQLPDLPIIEDDDVTVSELPKPMKNSRITMTKKPQKTSSGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRV3</accession>
    <name>T1GRV3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRV3"/>
    <dbReference type="STRING" id="36166.T1GRV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006397-RA">
        <property type="protein sequence ID" value="MESCA006397-PA"/>
        <property type="gene ID" value="MESCA006397"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136301_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006397-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12920" checksum="9CECD05E3C836DEA" modified="2013-10-16" version="1">MSPYKTSKETLKNVNRVAFRSLEEERRIDNAFTPIKDRTHSENIHPVHKDSEKYKKLTHQSFRISNSLEPPEPTTSSACNSKNMMWKDSRFYRKSKIAKSNDSLLTTGGGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRV6</accession>
    <name>T1GRV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-dependent kinase 7</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division protein kinase 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02039569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GRV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006400-RA">
        <property type="protein sequence ID" value="MESCA006400-PA"/>
        <property type="gene ID" value="MESCA006400"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000097">
        <property type="entry name" value="Putative cyclin-dependent kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006400-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="28705" checksum="B418774B61224DB8" modified="2013-10-16" version="1">MDTDLEIIIKDPKIILTQANIKAYMIMTLQGLEYLHMNWILHRDLKPNNLLVNTNGVLKIGDFGLAKFFGSPNRAYTHQVVTRWYRAPELLFGARQYGVGVDIWAVGCILAELLLRVPFLPGESDLDQLTKIFQALGTPTEETWPNMKDLPDFMQFKIFQGTPLTHIFTAAPDDLIDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCKEALDMPYFSNKPGPSMGPGKLPMPQSLKDKIAEERPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRX6</accession>
    <name>T1GRX6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Otopetrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006422-RA">
        <property type="protein sequence ID" value="MESCA006422-PA"/>
        <property type="gene ID" value="MESCA006422"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011508_3_0_1"/>
    <dbReference type="OMA" id="AWKIRTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF61">
        <property type="entry name" value="PROTON CHANNEL OTOPLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006422-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45506" checksum="AE8C01FFD2FAB659" modified="2013-10-16" version="1">MIRPSKAETRADALATLISALYGKLLVVMGVAFPMAEQIKVYKHKILARFGLMHMIGTNLAVWLNVLIQETKHEILTFYNPDNRNLSIFNRLGHRQQQQQAHMHHTVVSVDHNVTPIDASPFLFPCTIEYSLICAAILYVMWRSISRPQPEHRSRTESNIHSPLKRSPHHYSVDCARAHKGLFVGILILVLTIISLILFFVLISRAEFISLAVTEVTICELFIYATSSIATLIGMIQIRNLQYDAARSFELDDILLVGAQTGSFLYNTFTVIAGHFTLRKDTILILVNAMASIVQTAFQTMFILDASRRQAVKPEDIRKKPGREIVTFMLVVNLAMWAISTLEKSRAESHPIQLNFYGLWAWTIITHVSMPLAIFYRFHSTVCLCEIWKRAYKLKPTYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS36</accession>
    <name>T1GS36_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS36"/>
    <dbReference type="STRING" id="36166.T1GS36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006484-RA">
        <property type="protein sequence ID" value="MESCA006484-PA"/>
        <property type="gene ID" value="MESCA006484"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2090798_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006484-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="12038" checksum="BD68918C24D461B2" modified="2013-10-16" version="1">MIKDNLRKFFGLLIELGTILYILLKDFTNPSLPPGEPGADIITGAGGLMNANDLLLLAARQRQPLTGDKIVNGLMGNGGPGVIPQFALGIGAPLTGAYSSGIPSLSAFTLTNTGLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS41</accession>
    <name>T1GS41_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006489-RA">
        <property type="protein sequence ID" value="MESCA006489-PA"/>
        <property type="gene ID" value="MESCA006489"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1290297_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006489-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="214" mass="25408" checksum="4261E949AF7B8C0E" modified="2013-10-16" version="1">MKALNISKLLYKEEDEIKISCFLNTKIQTMAELNSEGVSSLITELCHHLCVKSENPNLKLIPKLKRKSLEIILHWRESDATELHLQSEWEQLKLKDRSKILQDEILENQLVNDAFTFMLALENSKNDEQENDDSERITTIFRPGPFTLHDWSYRKKENDLICKTENMFFAKPKYFQGVDKFDKDLYQEKNQEVNFMKERCFMKFLKPPDVPEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS92</accession>
    <name>T1GS92_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006543-RA">
        <property type="protein sequence ID" value="MESCA006543-PA"/>
        <property type="gene ID" value="MESCA006543"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078919_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006543-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13551" checksum="9BED867FBEB4BC1B" modified="2013-10-16" version="1">MLYINDFQVYDSLGPHSGGNAISAFYYKFLGLPSYLKSKILFIFVAILTLAKYDNSGIHLICGFKKGLNSTYYCRFYLTPKSICQHLIDVIGKCFSTSKEVCFYLNLKILEKHHKETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSN2</accession>
    <name>T1GSN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NR LBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSN2"/>
    <dbReference type="STRING" id="36166.T1GSN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006692-RA">
        <property type="protein sequence ID" value="MESCA006692-PA"/>
        <property type="gene ID" value="MESCA006692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485919_0_0_1"/>
    <dbReference type="OMA" id="GRVICIE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF473">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 75B, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10008" checksum="63AA678330CB4453" modified="2013-10-16" version="1">MRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKKCLQTIINENRPDQPEFMAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSN8</accession>
    <name>T1GSN8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSN8"/>
    <dbReference type="STRING" id="36166.T1GSN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006698-RA">
        <property type="protein sequence ID" value="MESCA006698-PA"/>
        <property type="gene ID" value="MESCA006698"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010718_0_1_1"/>
    <dbReference type="OMA" id="THMALRK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF13">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="128"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006698-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="380" mass="44264" checksum="BA779F7E067D45C4" modified="2013-10-16" version="1">MPELNPEWFSKDLLEDVLRKKYSSKNLEIKDFKVTNFGKGDNYASQMKRVYVEYSLNEELKSGHFLLKSCFEQDEFIMNILTPYNVFNHEMRMYDEILPRLSSILRSAGDPTNLSPETLAVFHKKGSILFEDLSKNNFTMEDRIKGLNFKQTKLVLQKVAKMHAASAVLKEKEPNVFENCDRGIFNKYTRGFSPYFEANTRICGELFSNMEGFEYIGEKLLKLVPHLMDYGEEAYQPDPSHFNVLYFQLSSWGSPAIDLFYLFSTSLQKDIRGRTDELIQYYYYELLNTLKKLKFKGKIPTLTELRSQLLMKSFIGFTAAIASQPVMLNSETEDADFHAMMKNDERGVKFKNLLFQNPLIQESLKSSLPYFERLGVFDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSQ2</accession>
    <name>T1GSQ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
            <location evidence="1 9">Extracellular space</location>
            <location evidence="1 9">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02060930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSQ2"/>
    <dbReference type="STRING" id="36166.T1GSQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006713-RA">
        <property type="protein sequence ID" value="MESCA006713-PA"/>
        <property type="gene ID" value="MESCA006713"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105018_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF97">
        <property type="entry name" value="PROTEIN WNT-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006713-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="28897" checksum="3C7229868A3DEAE0" modified="2013-10-16" version="1">MAVFNSTGKNAGPCRYITATRKQNHQCRKDLGLPDTLIEARRLAVTHCEDQFKYERWNCSIETRGKRNIFKKLYKETAFVHALTSAAMTHSIARACTEGRMTKCGCGSSNKNTHINAKSNWKWGGCNDNLKHGKRVTRAFLDLRGSSNVDEITELLRHDSEVFISYVLLFLKGYIFLGWYRNSIITAKDKCKCHGVSGSCSMKTCWKKLADFNETARALRHKYHQAVRKPTEIRMARSEFLAGGRLRRPRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSY7</accession>
    <name>T1GSY7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02064333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006802-RA">
        <property type="protein sequence ID" value="MESCA006802-PA"/>
        <property type="gene ID" value="MESCA006802"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624816_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006802-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8907" checksum="F2993CD38BAE8029" modified="2013-10-16" version="1">MFPSPQNLSANRLYRIHKNGQNNFQVGRKLKLYESAKFLGVFSTPSLNWKPHKQLYMPVEAVLGKHGLQLGQYSCMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTK9</accession>
    <name>T1GTK9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007044-RA">
        <property type="protein sequence ID" value="MESCA007044-PA"/>
        <property type="gene ID" value="MESCA007044"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3280064_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007044-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="4401" checksum="AAB41A1270EC200F" modified="2013-10-16" version="1">MTEVLSITLKGPQRPETWPVTAGGKFLLAGGPPRAKFHTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTM6</accession>
    <name>T1GTM6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02163586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTM6"/>
    <dbReference type="STRING" id="36166.T1GTM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007064-RA">
        <property type="protein sequence ID" value="MESCA007064-PA"/>
        <property type="gene ID" value="MESCA007064"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006383_1_1_1"/>
    <dbReference type="OMA" id="MRQYEPI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000001">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF65">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2, 6-BISPHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="227"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="457" mass="51407" checksum="3174C238B23A2860" modified="2013-10-16" version="1">MFLRKMFHSARRRGCSLDSSRGNSGVTGFNNHSQTPCPLRAPLVVTIVGLPCRGKSFAAHKVARHLCWKGELAKVFTVDSDATTETLSDVLEFFKNGNNVAIIDGMHLTRQSRQFIASFCTERIYHHLIVEFDSDAKSVESNINEMTNFFAKQNQANADIAGPKIQDVTALYERCEASESPMITVHNSSNPILHSVTARAVQGALQTSILGVLSHPVIHEQLFYFSRHGESEYNVLGRIGGNADLSPRGWKYGQRLAKFFRENQKCYPGPQLIWTSELQRTVHTAQDIPAPRAAIGDLNEINAGICEGLTYEEIQEQFPQEFAWRDQDKFKYRYPHGESYLDLLQRVDSVVQGLLTKTQVLCVSHQAVLRCIMAYFHGTKPDQVPYINVPLHTLLVVRTNGFNFEVEKVPLQVECVDTYRVKPKNCNVERTSSDALDTVPAHFDAPLPKVVQELTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTP1</accession>
    <name>T1GTP1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Runt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTP1"/>
    <dbReference type="STRING" id="36166.T1GTP1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007079-RA">
        <property type="protein sequence ID" value="MESCA007079-PA"/>
        <property type="gene ID" value="MESCA007079"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006530_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013524">
        <property type="entry name" value="Runt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF31">
        <property type="entry name" value="SEGMENTATION PROTEIN RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51062">
        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Runt" evidence="5">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007079-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13898" checksum="2B5FC535B8A8723F" modified="2013-10-16" version="1">MVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGERPPPHQELKRLYQNPDVVQKTLTSDVDSSFATSSAYAKGVFCHRVLTKSVETEHRASTTLKEAKLAETRPAKPEFIAQVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ6</accession>
    <name>T1GTQ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02012083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02012084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ6"/>
    <dbReference type="STRING" id="36166.T1GTQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007097-RA">
        <property type="protein sequence ID" value="MESCA007097-PA"/>
        <property type="gene ID" value="MESCA007097"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2339509_0_0_1"/>
    <dbReference type="OMA" id="IERCINV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007097-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11223" checksum="21CF0A6FAA3D206F" modified="2013-10-16" version="1">KGLQDEGLYRKSGVSTKITKLIQLALDKNTTDSPFCNEDTYKDLLDSNTVANALKTYLRHLREPLMTFQLYEKFINAAKNESVPIRIFEVHKLSCSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUL7</accession>
    <name>T1GUL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007433-RA">
        <property type="protein sequence ID" value="MESCA007433-PA"/>
        <property type="gene ID" value="MESCA007433"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375153_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007433-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10339" checksum="CDA66627E9A1B908" modified="2013-10-16" version="1">MGFPKSYFLLPSQIHKLPPLVLKSFLRDLVSKDSICISYPLGGGLTPPRGTIWKRNSLISAKLAGGGRHKLLGTHLSHGVLATVDAYYFIVYGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVI5</accession>
    <name>T1GVI5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007791-RA">
        <property type="protein sequence ID" value="MESCA007791-PA"/>
        <property type="gene ID" value="MESCA007791"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471624_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007791-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9765" checksum="98D099A46E6280BB" modified="2013-10-16" version="1">MALAGGYLICINKEEHLKTEVPGETFSIMQNLTEALFSLDETSYALETVGDSTCIFHTIQRISESHLAEFDSGKAHLQLEYNTLGANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVI8</accession>
    <name>T1GVI8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVI8"/>
    <dbReference type="STRING" id="36166.T1GVI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007796-RA">
        <property type="protein sequence ID" value="MESCA007796-PA"/>
        <property type="gene ID" value="MESCA007796"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1273518_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007796-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="23591" checksum="D0747C1600AEEFA1" modified="2013-10-16" version="1">METAFSSIQFIITSQHFHHGKMKIRCAAHIHDVYWQTTEKSIEEDRHLYQSVHGNTIDFMSRSYDSESSDENNYLTDLHIQADETACSTADETSCSTADETACSTADETACSTADKIAFSTAGDVSSLNAANNTSETMGKLSRTTTIVISLIFIAISQVLVKIHLSLLLLPSVSATQKQLSERISSSSSASSSSSSSSATSATRSVRKMELHQFINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVR0</accession>
    <name>T1GVR0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVR0"/>
    <dbReference type="STRING" id="36166.T1GVR0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007876-RA">
        <property type="protein sequence ID" value="MESCA007876-PA"/>
        <property type="gene ID" value="MESCA007876"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888356_0_0_1"/>
    <dbReference type="OMA" id="NWNKYFL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007876-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7308" checksum="03DF14D9DE16D6EA" modified="2013-10-16" version="1">MNEVLSRDDMIELLGPRPFKEKSTYEEFVEGTGSLEEDTSLPEALKNWNKYFLIQGIQSIKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVU2</accession>
    <name>T1GVU2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVU2"/>
    <dbReference type="STRING" id="36166.T1GVU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007909-RA">
        <property type="protein sequence ID" value="MESCA007909-PA"/>
        <property type="gene ID" value="MESCA007909"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925270_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007909-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7150" checksum="454A63A744DE0B9F" modified="2013-10-16" version="1">MLKEITDLAGQRERVAETFEEKIIHGILILAKTLKDERKVTTQKTNMQINYRKLTIYNTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVZ7</accession>
    <name>T1GVZ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum metallopeptidase 1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02117514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02117515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVZ7"/>
    <dbReference type="STRING" id="36166.T1GVZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007965-RA">
        <property type="protein sequence ID" value="MESCA007965-PA"/>
        <property type="gene ID" value="MESCA007965"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007536_1_0_1"/>
    <dbReference type="OMA" id="RTHIFEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053973">
        <property type="entry name" value="ERMP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22248">
        <property type="entry name" value="ERMP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum metallopeptidase 1-like C-terminal" evidence="5">
        <location>
            <begin position="357"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007965-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="528" mass="60514" checksum="D32E8332C3F6C0D6" modified="2013-10-16" version="1">MRHYHQSVVNPFATTLGEEIFQAGLLPSDTDFRIFRDHGHVPGLDFDFMTTDSPIILNTTNLMKFLEEPFKTQEITCLLWSIANGKELEDPEPYAEGHVIFYDVCGWFLVFYTKTVGIALNYTFSVIGLIAITASVWSMAKKCGQSNLTVAKNFGIIFAIQLVSVLLAIGLNFAMAAFMDAVGSPLVWYGHPWALFGLYVCPTLFGLALLPSLYLEHTRKEILSLNAKSQLFAHSNCVVMILFTIIGTAMGIRSSYIFMLGVLFHTICIILNLLTMFQNKQFLWIFWNIMSQILPFLYFAYLGTFLIRTLIPMKGRGGMSGNPEIHICLLCFVFTWLATGFLTPVLYLFKMTRTITLNKIISENKNLILNEIGEDCKNEVLCKFPFFNHRWFKNRNSAYYFKAETQPKLPYFPKLTLKNKQTSSGKVKYEFQLTGPHHMGIFLAPVENSKISSWTFNTTMLDKNWKPPYFMYMSWGVDSSPYEFFVELETQSSNEPVLEIGIGGHWIFEPELFTEEYKQFLGLFPKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW09</accession>
    <name>T1GW09_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW09"/>
    <dbReference type="STRING" id="36166.T1GW09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007977-RA">
        <property type="protein sequence ID" value="MESCA007977-PA"/>
        <property type="gene ID" value="MESCA007977"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_2_1_1"/>
    <dbReference type="OMA" id="DSHAIAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF4">
        <property type="entry name" value="FI01423P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="91"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007977-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="24064" checksum="6F2813D7B7868F61" modified="2013-10-16" version="1">MSKPILYGIDPSPPCRACLLTAKAIGLDFEYKVVNLMTGEHMTEEFLKKNPQHTVPVLEDNGSFITDSHAICTYLVQKYGSEKDQLLYPADLYTRALVDQKLHFDTGVLFQKVKGVTKYVMTGKKVPEEAVSAVYEGYDFLEKFLANSNYLVGNSLTVADLCCIATISSLVFAPIDPSKYPKLTEWIKRMKVLPYYETNEKGASQLLAMIKPSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW19</accession>
    <name>T1GW19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007987-RA">
        <property type="protein sequence ID" value="MESCA007987-PA"/>
        <property type="gene ID" value="MESCA007987"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718228_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007987-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="385" mass="43218" checksum="A6987C8BA640AA56" modified="2013-10-16" version="1">MDEKNAKSNVGTKEDAKNGPSKKPGTSKDASQESRKNIKPDPDEDFKRRGKRNRNHRKDDQKRPRFDKPKDTQTRYKLRGASKNYNKPLATTKIPLDDPYWLPCYENIPLPSAFGPGVFFIDQFVILSDNVRANLRFFEDGTIIVERRGETICLAKPESAFIAWRRCEQCTKNDHTIQSCPNVSCFLCGQKGHVGTSCKDPILGPFCLACRQKDHTHNQRAKCARLVYGPKMGRVCCLCGGRNHSVDNCRQSKCGLCGDSGHIGNACTKVLPPVLANAQYLPDQDLDSDEAVLQCMLQVKIGQYTYAAVCNDQDRFSEINPVHVRIKGFDGRNPAVRIPVRIGNIIHEVTFFVNGELPTPVSLGLDALHQFEIRIFIGEVQILDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW22</accession>
    <name>T1GW22_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.256</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc(2)Man(9)GlcNAc(2)-PP-Dol to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = a alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29543"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19512"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="ChEBI" id="CHEBI:132523"/>
            <dbReference type="EC" id="2.4.1.256"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:29544"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALG10 glucosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.256" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02053083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW22"/>
    <dbReference type="STRING" id="36166.T1GW22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007991-RA">
        <property type="protein sequence ID" value="MESCA007991-PA"/>
        <property type="gene ID" value="MESCA007991"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017053_1_0_1"/>
    <dbReference type="OMA" id="VIMESIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106073">
        <property type="term" value="F:dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016900">
        <property type="entry name" value="Alg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989">
        <property type="entry name" value="ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989:SF10">
        <property type="entry name" value="DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04922">
        <property type="entry name" value="DIE2_ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007991-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="331" mass="38025" checksum="85E734E5381EFAF6" modified="2013-10-16" version="1">MISLIGSVINVVFILRIRNRGDETNIWNMILDTFNISILPPILGLYYYWSEKKHFKATVAGAASILMRQTNVIWVVMVIGITSLDELCKNYGLAKRVKPSAVDIFKPYTFYDLLINYPLVIMESICNILRQYHIYISVIASFLVFVIFNGSIVVGDKSAHQAVIHIPQMFYFAIFSLIFGAPAFKDYMKPTLNSLATNWKLALRLTALISIAVAYNTLVHPYLLADNRHYTFYIWNRFFGKYSWFKFTMIPAYVFGLFLCGNIIKHKPKNFQVMFWICSFVVLVPQKLLEIRYFLIPFLLLRLNCAKVGRKASINFFETSLTSSSLSSVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW45</accession>
    <name>T1GW45_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW45"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008016-RA">
        <property type="protein sequence ID" value="MESCA008016-PA"/>
        <property type="gene ID" value="MESCA008016"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963408_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008016-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6928" checksum="57A3AA20783237DD" modified="2013-10-16" version="1">MELEHNLPSPQKSINRKNPNELSQKVLLTYTNPLATDFVLTANYHRLFNLSSRDYESMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU5</accession>
    <name>T1GWU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glucuronosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189746">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWU5"/>
    <dbReference type="STRING" id="36166.T1GWU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008276-RA">
        <property type="protein sequence ID" value="MESCA008276-PA"/>
        <property type="gene ID" value="MESCA008276"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012949_2_1_1"/>
    <dbReference type="OMA" id="ISWIITH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008276-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23277" checksum="3A392BFACF78EA05" modified="2013-10-16" version="1">MYINIISQHLGEFLDTAENGAIFFSLGSTAKSSDLSPETVKLFFNVLSKLPQKILWKWDDVENVPGKSDNILFDKWVPQNDVLAHKNLRLFITHGGKGSVVESQYHGVPMIVIPLFGDQTFNAKEIEGKMYGISINHKTVTKEHFEKVVNEVLENRKYLTNVKLFSKVYKDRPITAKDNAVFWMEYVLRHKGARHLQSPAGELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX82</accession>
    <name>T1GX82_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008424-RA">
        <property type="protein sequence ID" value="MESCA008424-PA"/>
        <property type="gene ID" value="MESCA008424"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3016597_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="56" mass="6685" checksum="982027F94C78C548" modified="2013-10-16" version="1">MLYNRKLPPPQPVRHIVEPLKKHIFSHQLRPKESHVQELNLKVQLISTLKLQLQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXA3</accession>
    <name>T1GXA3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein heavy chain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXA3"/>
    <dbReference type="STRING" id="36166.T1GXA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008446-RA">
        <property type="protein sequence ID" value="MESCA008446-PA"/>
        <property type="gene ID" value="MESCA008446"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2216485_0_0_1"/>
    <dbReference type="OMA" id="VKWMSKS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF70">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 2, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008446-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12482" checksum="0993FBF9BE379127" modified="2013-10-16" version="1">MTPELEVIIQSMNYKKIPVKWMSKSYPSLKTLGSYITDFVARLNWLQKWYDVGKPPTFWISGFFFTQAFLTGAMQNYARKYEIPIDTLTFDFQILTVKTKAHPAEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXG4</accession>
    <name>T1GXG4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02007692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008515-RA">
        <property type="protein sequence ID" value="MESCA008515-PA"/>
        <property type="gene ID" value="MESCA008515"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1760868_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF75">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN SB1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588584" description="Peptidoglycan recognition protein family domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="5">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008515-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="16935" checksum="95C9833CE6254191" modified="2013-10-16" version="1">MKMLKLTLLVFAIHSALALPIISRNECKTDLKNIQRDHKNNRKWSDIGYNFAIGGDGNIYEARGFGKQDPILTYACLVWLQALLKNVHYARLEGVLNSNHASRGVTKFKTYRSGGGKYHGQKVLLAYRVWVFPRKELWLQGDSLRISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXV3</accession>
    <name>T1GXV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXV3"/>
    <dbReference type="STRING" id="36166.T1GXV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008662-RA">
        <property type="protein sequence ID" value="MESCA008662-PA"/>
        <property type="gene ID" value="MESCA008662"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119591_1_0_1"/>
    <dbReference type="OMA" id="QCLKNEI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF68">
        <property type="entry name" value="FI14601P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="30"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14758" checksum="80DEB662350C7CC4" modified="2013-10-16" version="1">AYGYDSYNGYVTTSTNHLISTSQAPVRVVKRRNTANKKERRRTLSINTAFSSLRNRIPNVPSDTKMSKIKTLKLAILYIEYLGKILDGGQDPSNEFKAELKPLSRRSHSERKALQKNELQVIKKNIHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYE6</accession>
    <name>T1GYE6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYE6"/>
    <dbReference type="STRING" id="36166.T1GYE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008871-RA">
        <property type="protein sequence ID" value="MESCA008871-PA"/>
        <property type="gene ID" value="MESCA008871"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_974077_0_0_1"/>
    <dbReference type="OMA" id="WYVGTEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048919">
        <property type="entry name" value="Bdbt-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040478">
        <property type="entry name" value="FKBP_N_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21603">
        <property type="entry name" value="Bdbt-like_TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18023">
        <property type="entry name" value="FKBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BDBT FKBP like N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Bride of doubletime-like TPR" evidence="2">
        <location>
            <begin position="122"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008871-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="277" mass="32118" checksum="168D46F5CB6E3F97" modified="2013-10-16" version="1">MPIPKFTEKWEDTKKGIGKTTISFDSKHLIAPTEVSKVSVIFSNVQNLENRKSKFLEKIDSKVEIQMGSALNPIDFYIEILLGHLKKGELAKCFIKTSAMEITFNIKIIEIENVKHLFDLKLKEIFELAKVYKANGVEMYKKYPKFAEDYFCKGAKLMLSCKPFDHLTEKDDGVEGKDFLNSFLISTITLQLIRDGKFEDVVYLTQSVEESVENASEKAVFRRALALCNLQKHEEAKNILDQFPNKNNEMLKLYKRIQSEWKVSDEKYANMVRKMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYG5</accession>
    <name>T1GYG5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYG5"/>
    <dbReference type="STRING" id="36166.T1GYG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008892-RA">
        <property type="protein sequence ID" value="MESCA008892-PA"/>
        <property type="gene ID" value="MESCA008892"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_6_2_1"/>
    <dbReference type="OMA" id="XTFHIRI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000079">
        <property type="entry name" value="Calcium and integrin-binding family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051433">
        <property type="entry name" value="CIBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791">
        <property type="entry name" value="CALCIUM AND INTEGRIN BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791:SF6">
        <property type="entry name" value="CALCIUM AND INTEGRIN BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="13518" checksum="0D3E4EFA043692B9" modified="2013-10-16" version="1">MPKKDCTFFTRKEILRVHKRFRELRPDLVPRQMNEGQAASVKVPRECIEKLPELRENPFRTRICEAFSRDGQGNLSFEDFLDFLSVFSEQAPRDIKVFYAFKIYELSFITFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYP2</accession>
    <name>T1GYP2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYP2"/>
    <dbReference type="STRING" id="36166.T1GYP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008978-RA">
        <property type="protein sequence ID" value="MESCA008978-PA"/>
        <property type="gene ID" value="MESCA008978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191888_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF86">
        <property type="entry name" value="CCP84AC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="8755" checksum="A74652C5B5944DFC" modified="2013-10-16" version="1">MEKLPMVKNSKTQEETRDGDVVRGSYSLIEPDGSRRTVNYFADSINGFNAVVSKDLPVAVAPVAPVVAKQVVAPVVAKHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZE5</accession>
    <name>T1GZE5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SNARE-complex protein Syntaxin-18 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381040">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZE5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009244-RA">
        <property type="protein sequence ID" value="MESCA009244-PA"/>
        <property type="gene ID" value="MESCA009244"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657290_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019529">
        <property type="entry name" value="Syntaxin-18_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10496">
        <property type="entry name" value="Syntaxin-18_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SNARE-complex protein Syntaxin-18 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009244-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8381" checksum="351255671E4B0DCA" modified="2013-10-16" version="1">MDITAVFKASVMAVKLRNKQVAAMGGATTANGHEKISTEKRISPRHRSDPNALQAKDICHKITELKNFFSTIEQPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZS3</accession>
    <name>T1GZS3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009378-RA">
        <property type="protein sequence ID" value="MESCA009378-PA"/>
        <property type="gene ID" value="MESCA009378"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009378-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="13048" checksum="E3F43A889DF8E57E" modified="2013-10-16" version="1">MTWKRYVNQAAKKYKICGQPEECITPEHFWCSCPGLSVKRMKFLKTNSINCLEEQKSIDGECKINFILNSAVIDRPQPTNHSSPLVGVAGLKSIKGWCGYLKFNLMMCGIRYGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Z4</accession>
    <name>T1H0Z4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Z4"/>
    <dbReference type="STRING" id="36166.T1H0Z4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009842-RA">
        <property type="protein sequence ID" value="MESCA009842-PA"/>
        <property type="gene ID" value="MESCA009842"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763433_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009842-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="17088" checksum="5A3CCB2DF19A7057" modified="2013-10-16" version="1">MFMPMAWGRKNLCSPVRTWTSYGCEITICDANLSEQNLAEYSKHYEIGGVFDLYKTYYSDIKLLDKLSRISFPKVEYFVVFGYSSEENVSKKDVIFDRILYGLSGKFVQMDMDNISSCISDLDYLLGNIYNEVSRKVISKDHSSVETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H173</accession>
    <name>T1H173_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H173"/>
    <dbReference type="STRING" id="36166.T1H173"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009927-RA">
        <property type="protein sequence ID" value="MESCA009927-PA"/>
        <property type="gene ID" value="MESCA009927"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_633987_0_0_1"/>
    <dbReference type="OMA" id="AANHIHK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF136">
        <property type="entry name" value="KEKKON 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="202"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="338"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009927-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="433" mass="48433" checksum="C87FB35D0BAFCE98" modified="2013-10-16" version="1">MSGNNLQTLSKDQFIRANLLNLQKLFLRNCKIGEIEFETFNGLTNLVELDLSYNLLVSIPSEALTFLPSLRDLTIAANHIHKIDAHTFEKIPSLHELDLSHCDIQTIAPQAFEGLNSLTSLKLNGNKLTELRPKTIETFGQLHAIELHDNPWVCDCRLRAAKLWLTEKNIPYPVAPVCSGGPERIIDKTFAELHVDEFACRPELLPINRYVEAYLGENASIVCRASAIPSPVINWYWNGRLLSNGSAFSPYQRIYVYEDGQFEKKSRLILTNSQETDSIRLRRPTFLETKTPNHLEVIKNVNQNQSTSGSNKSHHQHQQNVNGGVVIANGGIINSTLETDTLERKKNDDSSFTNPLQKPPRLTTDIHPTTSTANAVNDCCSSTIASPTGSGNNPDLINDTRRFGDDEEFADLKIPAVLTTNINELTKTGEYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H242</accession>
    <name>T1H242_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acyl-CoA oxidase/dehydrogenase middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02073270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H242"/>
    <dbReference type="STRING" id="36166.T1H242"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010274-RA">
        <property type="protein sequence ID" value="MESCA010274-PA"/>
        <property type="gene ID" value="MESCA010274"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840129_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="4">
        <location>
            <begin position="95"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010274-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="14893" checksum="9510EB47D0A1AFA1" modified="2013-10-16" version="1">MIVPALRTLFPRSNGVLRQFQNSRKIACLASLSETHQMLQKTCRDFTDKLSRGSASVGVISAVTQLYLSFIQDNATEAQKEKFLTPFTATGDVCSFALSEPGNGSDAGAASTTAVLDGNNYVINGTKAWITNAAESVVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H245</accession>
    <name>T1H245_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02103107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H245"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010277-RA">
        <property type="protein sequence ID" value="MESCA010277-PA"/>
        <property type="gene ID" value="MESCA010277"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363405_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="8">
        <location>
            <begin position="40"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010277-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="22288" checksum="3110859F78930C95" modified="2013-10-16" version="1">MKRLRVMTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTWNSFIATSGSRDGSIIHHDVRHREHKVATLNGHSQEVCGLKWSTDYKYLASGGNDNLVNIYQAVPGGSTTNSEPLHVLNQHQAAVRALAWCPWQPNLLATGGGTADRCIKFWNGNNGSLLNSVDTKSQVCALLWSKTYKELISAHGYANNQLTIWRYPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2F9</accession>
    <name>T1H2F9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02030815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H2F9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010404-RA">
        <property type="protein sequence ID" value="MESCA010404-PA"/>
        <property type="gene ID" value="MESCA010404"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_759278_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010404-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="365" mass="42637" checksum="4C16F1B83B41E07A" modified="2013-10-16" version="1">MSLGPKEVNFNEVWGNIRETAKAVITLENVNREVWNNRFSDVYQLCVALPEPLADKLYFETKQFLENHVKNMLITRVQMNSNYNNDSGKFDLLQSYFDAWSEYNTEFVYGSQSPNHQEQMEIGELGLDIWRTYMIQSXXXXXXXXXXXXXXXXXXXXXXQSLGDKLVKQILEGIKADREGDPSITIQRAQVIHGVIHSFVQVQDYKQRSSLKLYQELFEIPMIIASGEYYRNEAAKLFQTCTVSEFMEAIIIIQEEETKRAQKFLHSTSAVKLRKECEEAFVNEKLDFLYSECKDMVSQERRKDLRNMYIILKPIPDNLKNILIQTFLDHIKNEGIETISTLKGENMHIQFVENMLKVIFFHLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2I6</accession>
    <name>T1H2I6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2I6"/>
    <dbReference type="STRING" id="36166.T1H2I6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010434-RA">
        <property type="protein sequence ID" value="MESCA010434-PA"/>
        <property type="gene ID" value="MESCA010434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576573_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9733" checksum="66B4C8D2C94EAB0F" modified="2013-10-16" version="1">MRSPKDQEKRDKNTIACRMSRRLKKLEEVAIEEQYKTFKDQYMANLLETQRIVHYMKELIKLSYPFMSSLPKYVGNITLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2T9</accession>
    <name>T1H2T9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Complement component 1 Q subcomponent-binding protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAM33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2T9"/>
    <dbReference type="STRING" id="36166.T1H2T9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010544-RA">
        <property type="protein sequence ID" value="MESCA010544-PA"/>
        <property type="gene ID" value="MESCA010544"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083914_1_1_1"/>
    <dbReference type="OMA" id="TRTLWIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010544-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23192" checksum="D7E1260B7A908ACD" modified="2013-10-16" version="1">MSALMNATRKFMGISGALKNVINKREFTRTLWIMSKTPETQTHSIGCSCGCGAKHMHTKGERELVEFLTEEILAERKAQKIKTIPTEIDGFKVKLDGADVELTKQDAKEKIVIKFNVNHTVDTEEAEAEVDAQNDKADFGEMKSKPQFEVDIVRGNTTLSLTCSFLNEPAQEGEYNDVFGIDEMTIFNGEWNDKVYAVAGDVLDGVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H302</accession>
    <name>T1H302_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H302"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010610-RA">
        <property type="protein sequence ID" value="MESCA010610-PA"/>
        <property type="gene ID" value="MESCA010610"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2612864_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010610-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9396" checksum="38F6F1E2BC18A966" modified="2013-10-16" version="1">IEANKIYLSNGNPYLKYLKGIPCKIKQNKWDIVLKGNEEVCFPTYINIPYMDEMIHCQSALILYEVYRIYDSIKYQIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3K0</accession>
    <name>T1H3K0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02167378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3K0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010828-RA">
        <property type="protein sequence ID" value="MESCA010828-PA"/>
        <property type="gene ID" value="MESCA010828"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375170_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012813646" evidence="1">
        <location>
            <begin position="16"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10728" checksum="A5A149035685FF2E" modified="2013-10-16" version="1">MKLLVLLSIVSIVATQQTIKYSNPKPVTISVYNAQFPKPRGILRPTLLPDKLFSPAVNFVDINKKSLTSESIRVTKSTYQSGYIYEKPTYKSFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3K4</accession>
    <name>T1H3K4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3K4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010832-RA">
        <property type="protein sequence ID" value="MESCA010832-PA"/>
        <property type="gene ID" value="MESCA010832"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3360239_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010832-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="36" mass="3775" checksum="71FB7F7B3C2B9769" modified="2013-10-16" version="1">MFKITLAIFAVIACAAAKPALVAPLASLYQSLLPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3K7</accession>
    <name>T1H3K7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3K7"/>
    <dbReference type="STRING" id="36166.T1H3K7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010835-RA">
        <property type="protein sequence ID" value="MESCA010835-PA"/>
        <property type="gene ID" value="MESCA010835"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037252_1_0_1"/>
    <dbReference type="OMA" id="VFKWIMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042507">
        <property type="entry name" value="TBC1D19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16110">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16110:SF1">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="154"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010835-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="413" mass="47982" checksum="D0053F580D0E78EB" modified="2013-10-16" version="1">MNKSDPLAYLRRAGVQWDRRVRKSLNSMCNELKMPLQGQMRLPSDKEELQSKWDELSNYAVDLSSYRPVYAPKDLLDVLLSLKGPAKGDNGENIPKWEFSHIQLPVKNLMDLRNHYNDLLRSDNTSSLAISTQRQLILQYKHSPLCQSILKKGNTPPIYRGEIWAYVLGSHLNFNESEHWEKLKNHVLNTDHIVDKLIFKDVQLTASNDDQYFVFEDVLYQVMLCFSRDTEINDMIQFEMYPVKGKSYEGPPSGVVPFHGFVSPFCYLYDSPKMLYFSLRAFYIRYCHRLTTINTHPQGIVSLCLLFEKLLQTHEPELWSHFRELEIQPIRVVFKWLMRAFSGHLPPDQLLILWDLVLGFDSLEILPLFAIIIISFRKDSLLQVTSLDCIEAILADLSSIKVLPLIQLALSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4F4</accession>
    <name>T1H4F4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036598">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4F4"/>
    <dbReference type="STRING" id="36166.T1H4F4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011163-RA">
        <property type="protein sequence ID" value="MESCA011163-PA"/>
        <property type="gene ID" value="MESCA011163"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388734_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10445" checksum="EA5DB76C0C44CC97" modified="2013-10-16" version="1">MMKVESTTENSENSVLKRQTVTEEVSVADLEDSFGKGDINKDNQSQLPAKKKNGFYLLADWNSFLEVGDGDEKVVVRLNKTIGDPSLFIPVKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4H7</accession>
    <name>T1H4H7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 24</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4H7"/>
    <dbReference type="STRING" id="36166.T1H4H7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011186-RA">
        <property type="protein sequence ID" value="MESCA011186-PA"/>
        <property type="gene ID" value="MESCA011186"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_931505_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021429">
        <property type="entry name" value="Mediator_Med24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11277">
        <property type="entry name" value="Med24_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011186-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="299" mass="34291" checksum="90EB32DDDD849F32" modified="2013-10-16" version="1">MLILTINLIEKITKNEFIMGIYYIGKQEDMEISNKIAMKHNLIKKYSHITKQYTFLEAKLLLLTSLDSSNLEMKRLTEPQVESICYCTQPLLSMEIYLHLSADTQYYVSEVLMIQKLKNYSMSRLMYEILRACFISLSNINENTEDTSWAAFTFFKLPKIFKQLQAINSSNANPNLDYIPEVVEALKMLLDDTPLFDHLEQKCFGNNMEFLFKDWVKQKIINEKYCAHFQAERSAQSSQVMKQDVVSPIQSLITSVHRAAAPLTAIIKTLNSDSNKVQEGVCNVLGQALVGKLLCISII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4V3</accession>
    <name>T1H4V3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4V3"/>
    <dbReference type="STRING" id="36166.T1H4V3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011321-RA">
        <property type="protein sequence ID" value="MESCA011321-PA"/>
        <property type="gene ID" value="MESCA011321"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962114_0_0_1"/>
    <dbReference type="OMA" id="GACCQSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22247">
        <property type="entry name" value="MCM8_WHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056875">
        <property type="entry name" value="MCM8/REC_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25051">
        <property type="entry name" value="WHD_MCM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM AAA-lid" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="MCM8/REC winged helix" evidence="2">
        <location>
            <begin position="56"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011321-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14323" checksum="6CCA34EF90EA3697" modified="2013-10-16" version="1">MEGLIRLAQARARVELAIEVSVDHVKEVISLVKFSQTNLQVDEMEDVQKMARLPSGSSSRTSQLKKFLRLLETRASALGKTIFDRDELKDLATRGGITTGPTELIDVLNDQGVLLKKGNNIYEFLMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H553</accession>
    <name>T1H553_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H553"/>
    <dbReference type="STRING" id="36166.T1H553"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011428-RA">
        <property type="protein sequence ID" value="MESCA011428-PA"/>
        <property type="gene ID" value="MESCA011428"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078926_0_0_1"/>
    <dbReference type="OMA" id="NIAKTLW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011428-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13502" checksum="2A0F7A466BD5D583" modified="2013-10-16" version="1">NISESCSKDAKFFTLRSFKEFIVIAHNRHRNSLAGGSIPNHKSASRMGTVSWDDDLSYLSSLNVKRCKFGLDQCRNTEKFRASGQNIAKTLWDTRGSAISNLQTQIDKWFNEYKFSNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1H585</accession>
    <name>T1H585_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H585"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011460-RA">
        <property type="protein sequence ID" value="MESCA011460-PA"/>
        <property type="gene ID" value="MESCA011460"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778767_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588735" evidence="1">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011460-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7745" checksum="CD4A570BB899A0FF" modified="2013-10-16" version="1">MELFKFLIFVILSISLFSKSSSLPGNSLESTTPNSTKIKIQQSFTILSSPCRVGFRPDHNQKCRKIILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5H2</accession>
    <name>T1H5H2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02145573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5H2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011549-RA">
        <property type="protein sequence ID" value="MESCA011549-PA"/>
        <property type="gene ID" value="MESCA011549"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429586_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011549-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="9983" checksum="1861D6AC1BFE64F6" modified="2013-10-16" version="1">MRKTYSKKPEFTSSSSISTTTNPATTLNCPLIPMKDTSPKLTPPLSTIEIYSLFTPELNIPPSLKCVAHPKAIFLPGMAPDVSLEEVSIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5K7</accession>
    <name>T1H5K7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional purine biosynthesis protein ATIC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">AICAR transformylase/inosine monophosphate cyclohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:22193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>10-formyldihydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + 7,8-dihydrofolate</text>
            <dbReference type="Rhea" id="RHEA:59144"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57452"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:59145"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:18447"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Associates with internalized INSR complexes on Golgi/endosomal membranes. Interacts with INSR; ATIC together with PRKAA2/AMPK2 and HACD3/PTPLAD1 is proposed to be part of a signaling network regulating INSR autophosphorylation and endocytosis.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02022699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5K7"/>
    <dbReference type="STRING" id="36166.T1H5K7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011588-RA">
        <property type="protein sequence ID" value="MESCA011588-PA"/>
        <property type="gene ID" value="MESCA011588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_2_1_1"/>
    <dbReference type="OMA" id="CVNLYNF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGS-like" evidence="7">
        <location>
            <begin position="23"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="23339" checksum="D156E84B53E80C44" modified="2013-10-16" version="1">MKIVISYQNLVEVRISIGKSKEIVMASGAKLALLSVSDKTGLLEFAKALSGFGYQLVASGGTAKQLRAAGLVVRDVSDITGAPEMLGGRVKTLHPAVHGGILSRTTESDLKDMRAQSFDLIDIVACNLYPFVETISKPEVTIADAVENIDIGGVTLLRAAAKNHDRVTVVVDANDYSKILSEIQQTGNTLPETRQNLALKAFTHTASYDEAITDYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H660</accession>
    <name>T1H660_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NF-kappa-B inhibitor-interacting Ras-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H660"/>
    <dbReference type="STRING" id="36166.T1H660"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012193-RA">
        <property type="protein sequence ID" value="MESCA012193-PA"/>
        <property type="gene ID" value="MESCA012193"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_17_0_1"/>
    <dbReference type="OMA" id="IMDRANN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032794">
        <property type="term" value="F:GTPase activating protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032484">
        <property type="term" value="P:Ral protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042227">
        <property type="entry name" value="KBRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46152">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR-INTERACTING RAS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46152:SF3">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR-INTERACTING RAS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012193-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21984" checksum="2935D48A9AC2BAC3" modified="2013-10-16" version="1">MLTTKQSKSAKVLLCGMKNVGKTCLIEQLIYGNVTLETKYNPTIEDIYIASVDTGKSPRESLRIFDTAGIQGKAEYPKHYHQFPDAYILVYDPQDPASLDMLADIKSDIDRNKEKKEAVIIVLANVHSNVPDSGIDSHKANNWCAREKIKHYTVNAMERASLYEPFVSLTIRLHPAQSKSTFTQFSKVMQKTKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6D9</accession>
    <name>T1H6D9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL34m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">39S ribosomal protein L34, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6D9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012272-RA">
        <property type="protein sequence ID" value="MESCA012272-PA"/>
        <property type="gene ID" value="MESCA012272"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129938_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3980:FF:000001">
        <property type="entry name" value="Mitochondrial ribosomal protein L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_bL34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012272-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="49" mass="5985" checksum="5C72C9CA11AF671C" modified="2013-10-16" version="1">FNRAILKNKVRTHFPKPREIKRINVHGWETRMSTVEGRRTLMRRILKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6G3</accession>
    <name>T1H6G3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6G3"/>
    <dbReference type="STRING" id="36166.T1H6G3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012296-RA">
        <property type="protein sequence ID" value="MESCA012296-PA"/>
        <property type="gene ID" value="MESCA012296"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081098_1_1_1"/>
    <dbReference type="OMA" id="PFERILC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012296-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12395" checksum="9F6A2615E6201331" modified="2013-10-16" version="1">SSALFFQNTQLETAYHILIDTNFINFSIKNKLGIIQEMMDCLYTKIHCDPFERILCLHKGTNADNCLVNRVIQHKCYIVATKYKGLNKIRKIPGVPIMYVDSHKYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7I3</accession>
    <name>T1H7I3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60S ribosomal protein L8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7I3"/>
    <dbReference type="STRING" id="36166.T1H7I3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012720-RA">
        <property type="protein sequence ID" value="MESCA012720-PA"/>
        <property type="gene ID" value="MESCA012720"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_0_2_1"/>
    <dbReference type="OMA" id="HPYKFKM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="8">
        <location>
            <begin position="24"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012720-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="19650" checksum="1F648AF03395A231" modified="2013-10-16" version="1">KELFIAPEGMHTGQSLYCGRKATLQIGNVMPLSQMPEGTIICNLEEKTGDRGRLARTSGNYATVIAHNPDTKKTRVKLPSGAKKVVPSNNRAMVGIVAGGGRIDKPILKAGRAYHKYKVKRNSWPKVRGVAMNPVEHPHGGGNHQHIGKASTVKRGTSAGRKVGLIAARRTGRIRGGKKYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA56</accession>
    <name>T1GA56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA56"/>
    <dbReference type="STRING" id="36166.T1GA56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000102-RA">
        <property type="protein sequence ID" value="MESCA000102-PA"/>
        <property type="gene ID" value="MESCA000102"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062828_2_2_1"/>
    <dbReference type="OMA" id="WIKINLA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000005">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="11">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="12">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000102-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="14672" checksum="CE25E18D57C67F03" modified="2013-10-16" version="1">MSAGKAGKDHGKTKHKAVSRSARSGLQFPVGRIHRHLKNRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIVKKTDGGDDGGKLYSQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAP0</accession>
    <name>T1GAP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NHR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAP0"/>
    <dbReference type="STRING" id="36166.T1GAP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000299-RA">
        <property type="protein sequence ID" value="MESCA000299-PA"/>
        <property type="gene ID" value="MESCA000299"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1564647_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF8">
        <property type="entry name" value="NEURALIZED-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="13"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000299-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="19217" checksum="333294ADFFCE9E48" modified="2013-10-16" version="1">MNQNNFEISADSQISDDETNDGNAANQQFYTRFHDYHGDNIVLSDENTVAYRKSREIFLVEIEENERGWSGHMRLGLTQLDPKTMALSARGLPPFALPDLANLGSSWVYPITKFIEDNRSTTQHGTQGEGSNEGSRENGRKSSANHKLYSRHNGVNSFSTQVFPFPLSFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBE8</accession>
    <name>T1GBE8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02147737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBE8"/>
    <dbReference type="STRING" id="36166.T1GBE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000580-RA">
        <property type="protein sequence ID" value="MESCA000580-PA"/>
        <property type="gene ID" value="MESCA000580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039221_4_0_1"/>
    <dbReference type="OMA" id="TDHWKNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="146"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="31303" checksum="B2FEB8E38737409A" modified="2013-10-16" version="1">KFLIIFENKSLLYFPGQFLTGRVLLELQDDTPTLGLHFHVVGEGVVRAGTARQERTYDKENYIDFRMKLLGDTDQGPTVLSPGIHSFPFKLGLPTGLPSTFLGRYGWIQYYCKAALRETNGLIHKNHQVFIVMNPIDLNLEKPILATIEYHARGKVIQTEKRALAVLVRGKIRPGDKDEWQGDSLYVPPLPPTNLRGCHLIKISYDVFFVIEPKSLEKEIKVQLPIVLATYPFRSNANGSDITHTWPEAVLKPDTHYPSTLPIFRPWLHEKTEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBH9</accession>
    <name>T1GBH9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial-processing peptidase subunit beta</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02103929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBH9"/>
    <dbReference type="STRING" id="36166.T1GBH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000611-RA">
        <property type="protein sequence ID" value="MESCA000611-PA"/>
        <property type="gene ID" value="MESCA000611"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_4_2_1"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="53"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="235"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000611-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="453" mass="50382" checksum="50B164BD49AA974B" modified="2013-10-16" version="1">MALKVLFCANNLKQLTRGAQIIARSKSSYAPDLKKALLNIPATQITTIDNGLRVASEDSGAATATVGLWIDAGSRYETEANNGVAHFLEHMAFKGTAKRSQTDLELEVENLGAHLNAYTSREQTVFYAKCLSKDVPKAVEILADIIQNSKLGESEIERERGVILREMQEVESNLQEVVFDHLHATAYQGTPLGQTILGPTKNIKSIGKSDLQDYISNHYKASRIVFLQNTYDGKPPAVSPCRFTGSEIRVRDDSLPLAHVAIAVEGCGWTDQDNIPLMVANTLIGAWDRSQGGGVNNASTLARHSAESNLCHSFQSFNTCYKDTGLWGIYFVCDPLQCEDMLFNVQQEWMRLCTMITEGEVERAKNLLKTNMLLQLDGTTPICEDIGRQILCYNRRIPLHELEARINAVSVQNIRDVCMKYIYDRCPAVAAVGPVEALPEYGRIRSSMYWLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBV9</accession>
    <name>T1GBV9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000748-RA">
        <property type="protein sequence ID" value="MESCA000748-PA"/>
        <property type="gene ID" value="MESCA000748"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3433233_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000748-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="16" mass="1850" checksum="EDB7896F2A82CB0D" modified="2013-10-16" version="1">MPQTKFVAVYVAFHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC57</accession>
    <name>T1GC57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit g</fullName>
            <shortName evidence="10">ATPase subunit g</shortName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase g subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC57"/>
    <dbReference type="STRING" id="36166.T1GC57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000855-RA">
        <property type="protein sequence ID" value="MESCA000855-PA"/>
        <property type="gene ID" value="MESCA000855"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152793_0_0_1"/>
    <dbReference type="OMA" id="CIGKRHF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016702">
        <property type="entry name" value="ATP5MG_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12386">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017835">
        <property type="entry name" value="ATP-synth_g_mitoch_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 10" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000855-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11227" checksum="77D380BD97AE4484" modified="2013-10-16" version="1">MASIATKLLEFWLVNLPNQARPQLQTFLKYAKVELTPPTPGDIPAIRQGISKIVSGARTGAWKNVTVREAWLNTLVTAEVIFWFYIGECIGKRHIVGYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCT8</accession>
    <name>T1GCT8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCT8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001111-RA">
        <property type="protein sequence ID" value="MESCA001111-PA"/>
        <property type="gene ID" value="MESCA001111"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2280592_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001111-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="11617" checksum="DD159182199394A7" modified="2013-10-16" version="1">MQNAESDNSTKNISDDEPENLQRPINNEKDKDVQKQLLNMMEKEGRLSGEEMSKLRNVVLKDWAPGTEVKVVRANPKESEVISDESENNSNVQNFKSLKNNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDH1</accession>
    <name>T1GDH1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serum response factor homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDH1"/>
    <dbReference type="STRING" id="36166.T1GDH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001358-RA">
        <property type="protein sequence ID" value="MESCA001358-PA"/>
        <property type="gene ID" value="MESCA001358"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402182_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001358-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10224" checksum="62BC9323DE84BE57" modified="2013-10-16" version="1">MYIYGYDFKAYELSTLTGTQVMLLVASETGHVYTFATRKLQPMITSEAGKQLIQTCLNSPDPPNVGSGGDQRMSATGFEETELSYNIADEDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDL3</accession>
    <name>T1GDL3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDL3"/>
    <dbReference type="STRING" id="36166.T1GDL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001402-RA">
        <property type="protein sequence ID" value="MESCA001402-PA"/>
        <property type="gene ID" value="MESCA001402"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052006_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="8">
        <location>
            <begin position="195"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001402-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29353" checksum="83ACEFD2DCE234D0" modified="2013-10-16" version="1">VVVNTCGPYRFYGEQVVEACIKAGTHHVDVSGEPQYMETMQLKYNEEAAKKNVYIISACGFDSIPNDLGTVFVEKNFNGVVNSVETYLETGSSGNKSSGASLNYGTWESAVYGLTHAKELKGIRSQLFKDQSTISDIEVDTIEVKGPTSISVPGYEKNVTFGVLYDVAYKIIGTDEEIVVSTTRLKRSLEMWQSLNVDAQVWHPFRNEPIPIIFDLGVDKDFGTGAVKITPAHDKLDFDVGNRHLLNPIQVFTEDGRVDNHSVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDT0</accession>
    <name>T1GDT0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392995">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDT0"/>
    <dbReference type="STRING" id="36166.T1GDT0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001476-RA">
        <property type="protein sequence ID" value="MESCA001476-PA"/>
        <property type="gene ID" value="MESCA001476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_12_1"/>
    <dbReference type="OMA" id="WQENILA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002204">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF246">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 63E, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14057" checksum="8FEC71BB85F86510" modified="2013-10-16" version="1">LFQLLRGLSYCHKRRVLHRDVKPQNLLISENGELKLADFGLARAKSVPSHTYSHEVVTLWYRPPDVLLGSTEYSTSLDMWGVGCIFVEMVTGMPTFPGIRDTYDQLDKIFKLLGTPTEDTWKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEA7</accession>
    <name>T1GEA7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001665-RA">
        <property type="protein sequence ID" value="MESCA001665-PA"/>
        <property type="gene ID" value="MESCA001665"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760701_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001665-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7822" checksum="3392AB0E3CA02ABB" modified="2013-10-16" version="1">MDFTSPVVRVSICPGYFKKQIVQPPPVNPSRTKRAQVAMVSPQNFYRQHELPLTMSSNTNCPTSGLAERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEF6</accession>
    <name>T1GEF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine = phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25325"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-methylethanolamine phosphate + S-adenosyl-L-methionine = N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25322"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.103" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02017129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEF6"/>
    <dbReference type="STRING" id="36166.T1GEF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001718-RA">
        <property type="protein sequence ID" value="MESCA001718-PA"/>
        <property type="gene ID" value="MESCA001718"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039068_7_0_1"/>
    <dbReference type="OMA" id="WTRKIKD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000234">
        <property type="term" value="F:phosphoethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="8">
        <location>
            <begin position="59"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001718-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="30565" checksum="99924D96A826C21B" modified="2013-10-16" version="1">MPTLTQSVETQKFLDTVQYSHKGIHKYELIFGDGFISTGGLTTTQHLCEKYLDGKLPENPRILDVGCGIGGGSVYLADRYNATVVGIDLGTNVINICNERYANKKNVSFSVQDALFSEYKAGEFDMIYSRDAFIHVAEKEKLFKLFYKWLKPGGIVFISDYCCGEEEVLTDAFKAYVAQRNYSLTTWQNYEQFLRNAGFTAKGENVTEWFRSILQMEKDRLLEPKMKAKFLETYSVDEMEGLIAGWDEKLVTTTQGNHIWAVFFAVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEM8</accession>
    <name>T1GEM8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEM8"/>
    <dbReference type="STRING" id="36166.T1GEM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001796-RA">
        <property type="protein sequence ID" value="MESCA001796-PA"/>
        <property type="gene ID" value="MESCA001796"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329229_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF4">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="104"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001796-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="22194" checksum="D5C06A2112FDC8F0" modified="2013-10-16" version="1">MVGGSSGGEGALQTSGGSPLGFGSDIGGSIRMPAFYNGVFGHKPSSNIVSLDGIFPESQTGEQKSFNVIGPLSRFAADLKPVMKVIAGEKAKTLNLDEPLSSLEVMEAFIARCKEINPLLNCVVDNRFEDALKEAKEVDDLIESGKYTVEELKEQKPFLGVPISTKDNVGIKDLLLSAGIWSRREVRAEEDSEAMSLMRKAGAIPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF50</accession>
    <name>T1GF50_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02054393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF50"/>
    <dbReference type="STRING" id="36166.T1GF50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001979-RA">
        <property type="protein sequence ID" value="MESCA001979-PA"/>
        <property type="gene ID" value="MESCA001979"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212936_0_0_1"/>
    <dbReference type="OMA" id="DHALLEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000437">
        <property type="entry name" value="Beat-IIIc, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF8">
        <property type="entry name" value="BEATEN PATH IA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001979-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12152" checksum="0BBEB4B59C2004F2" modified="2013-10-16" version="1">MEDDTLYSVKWYKGRREFFRYTPKEKPSMKIFSHPGINVDQSESNESHVLLTSVSLNITGKFSCEVSADAPTFHTAFHTGEMEVVGKLYRTQGDLLWGSNISILFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFJ6</accession>
    <name>T1GFJ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02102080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002138-RA">
        <property type="protein sequence ID" value="MESCA002138-PA"/>
        <property type="gene ID" value="MESCA002138"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925248_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002138-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6482" checksum="4F536F6D5898F167" modified="2013-10-16" version="1">MNITTINGGVVKQGKAGTVPIPVRSSTISIIPNPNICSIFCENFVQSPTIELGLLIIKPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFY1</accession>
    <name>T1GFY1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02099527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002283-RA">
        <property type="protein sequence ID" value="MESCA002283-PA"/>
        <property type="gene ID" value="MESCA002283"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888143_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002283-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15672" checksum="3C09856431B965E7" modified="2013-10-16" version="1">MDTALIIIPSSMLKNKLDSASPLRSIHIVGNLLRFRLQLLLQMFRSIIWLSLMSLSRMPKWLIARNSSYVVGVLFAYLKSMKSVYDDSFVTVIPVIRIVMIPFTLIWILILNTLFIRATLMFLGKDQKFEYLLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGT3</accession>
    <name>T1GGT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02103594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGT3"/>
    <dbReference type="STRING" id="36166.T1GGT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002614-RA">
        <property type="protein sequence ID" value="MESCA002614-PA"/>
        <property type="gene ID" value="MESCA002614"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="OMA" id="HIFAEMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000024">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="32"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13967" checksum="179FB9CEB2D3E0C0" modified="2013-10-16" version="1">GGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL8</accession>
    <name>T1GHL8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002925-RA">
        <property type="protein sequence ID" value="MESCA002925-PA"/>
        <property type="gene ID" value="MESCA002925"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778760_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002925-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7843" checksum="93C609BF3F99DD0F" modified="2013-10-16" version="1">MEISVIKIVQRIITFLESFTRNTYIVKELAEAATKSTDDLIMHTRKSLDDMADSARKSYEDLSTTASNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHT9</accession>
    <name>T1GHT9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHT9"/>
    <dbReference type="STRING" id="36166.T1GHT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003000-RA">
        <property type="protein sequence ID" value="MESCA003000-PA"/>
        <property type="gene ID" value="MESCA003000"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015131_0_0_1"/>
    <dbReference type="OMA" id="MGYYSHL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06713">
        <property type="entry name" value="PDZ_tamalin_CYTIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052122">
        <property type="entry name" value="Intracell_Traff_Signaling_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963">
        <property type="entry name" value="GENERAL RECEPTOR FOR PHOSPHOINOSITIDES 1-ASSOCIATED SCAFFOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963:SF5">
        <property type="entry name" value="SHORT SPINDLE 6, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="233"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="416"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003000-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="616" mass="70131" checksum="5E8F565B99768F58" modified="2013-10-16" version="1">ATYILKSDRSTLTKPDEDRRRRTIIIEKKNSSYGFTLQSYGIHYKKEQEVEMITYVDYVEYDGPAYRAGMREGDVILSINGTDMEKADHKTLVNFIKGCENRMRMVVLFEDCVRKVELHMRYIQLQNVLRSKMNELERICLREREILDGKWKTHRASSSGLNTSTEIPTTVYQRPALSTEDVTARQATQGGNPIIPPPAQFMLTYQYLDPMYRYVVRPSTSASSGEFIVPVDTEEPQNDMERRASQPEATMQANEKENKTKHKSRHCHGHSCNPCIGHFSKKSPPLNGSNGNGDNVSLDAYDLASPCCNAQCVPTKKRTKHHKEHHHKHKHRDKELKTEKSRSKSQTNPQPPHHHHHHYSSAQHEHFRQQAQRQRNYDLSVGLASHCSLHTCGSNSEYAPQDSTASYTTSLSTDTLYWDPQSEGHPSRQPSTKSRQSSYHPTTQQPTIQHYTFTPIYQPIMSAANRANVNYGYLDTVPRMPNCKPHPPHYQIGKNVYGRYSTYGDPGVENYAPPPSQFLEERITTTTTITTKSTENLLENESASGGGGGGSLNRRCECMKEIKTPQFQLQHDPHAVHTHRQCRVGYYSNLPRPLTNASTNMREDGVAATISEITRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI43</accession>
    <name>T1GI43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134462">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI43"/>
    <dbReference type="STRING" id="36166.T1GI43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003110-RA">
        <property type="protein sequence ID" value="MESCA003110-PA"/>
        <property type="gene ID" value="MESCA003110"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392227_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003110-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10825" checksum="12D6C0CE3AEF03F9" modified="2013-10-16" version="1">MSVQKVFRKNPIEDRRRDHLKQTWIRLMEGDDDRKSQYTCVAENSKGKREVTAEITVESPQGPPRLQFEPYNMDAFTGTTIELPCKAEGDSPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIG9</accession>
    <name>T1GIG9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 179</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM179 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003247-RA">
        <property type="protein sequence ID" value="MESCA003247-PA"/>
        <property type="gene ID" value="MESCA003247"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103794_1_0_1"/>
    <dbReference type="OMA" id="AQASLWC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029673">
        <property type="entry name" value="TMEM179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059010">
        <property type="entry name" value="TMEM179-179B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26158">
        <property type="entry name" value="Claudin_TMEM179-179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003247-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20380" checksum="714A0AB7AB05C293" modified="2013-10-16" version="1">MVLANFLILSQIAGHVILAILSLLVIIPMSMNLNEFNHHCILFATGNWREDDGLFEVRWPSAGFCKFPIFTGYFVLIVSILQIYKFGRLTHKNEEGGFLGLFIDVLLGIFMTMFGIISAIMVTLGFIVWCSDMTERFPSCDMAAGQNITDQSQNIDTSGFYFEMGTSQYIVTEHKLIILVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ48</accession>
    <name>T1GJ48_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GJ48"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003490-RA">
        <property type="protein sequence ID" value="MESCA003490-PA"/>
        <property type="gene ID" value="MESCA003490"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_935603_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003490-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="298" mass="32609" checksum="3035F061C012FE3F" modified="2013-10-16" version="1">MPLPQSPVVPLVTAVEFSICHCHKAHHRLCPISRQLEQDRXNNATATVTSSTTSNSSRILDLPLPQSTPSSVPYQSATGARPKVPQSASMSTTLANSGGASSSAADSLSSKMKRSPRFSSFDSQASLAEAATSDLPTSSQQNYANPTVENAYDHVQRSYSHYDMDYPRSRNLNGHRRNSPTSTSPHNRDRASNPEFAAAALPDFVQDHWMDQWYYKDHQHQHNLNSPPNSPTSPIRIIPFMEDFADVNPQPQTLPDFLSDGPIIHSSGRLADVASGLPNLDSPEDDSPNLLASRLQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJD7</accession>
    <name>T1GJD7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aralkylamine N-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.87</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dopamine + (9Z)-octadecenoyl-CoA = N-(9Z-octadecanoyl)-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51380"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31883"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:51381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dopamine + acetyl-CoA = N-acetyldopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51388"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:125678"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:51389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dopamine + hexadecanoyl-CoA = N-hexadecanoyl-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51376"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:134058"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:51377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>serotonin + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = N-[(5Z,8Z,11Z,14Z)-eicosatetraenoyl]-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:132255"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:51397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>serotonin + (9Z)-octadecenoyl-CoA = N-(9Z-octadecenoyl)-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51392"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:134064"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:51393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>serotonin + acetyl-CoA = N-acetylserotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25217"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17697"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
            <dbReference type="EC" id="2.3.1.87"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:25218"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>serotonin + hexadecanoyl-CoA = N-hexadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51384"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:134059"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:51385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>serotonin + octadecanoyl-CoA = N-octadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:134065"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:51401"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the acetyltransferase family. AANAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.87" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02134837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJD7"/>
    <dbReference type="STRING" id="36166.T1GJD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003583-RA">
        <property type="protein sequence ID" value="MESCA003583-PA"/>
        <property type="gene ID" value="MESCA003583"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085834_2_0_1"/>
    <dbReference type="OMA" id="MECIYSI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000046">
        <property type="entry name" value="Dopamine N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905:SF1">
        <property type="entry name" value="AT07410P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905">
        <property type="entry name" value="N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="5"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003583-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="23749" checksum="A93B2C313F3EA28F" modified="2013-10-16" version="1">MQTIITYRLAESKDEPSVLKLLREHFYTDEPLSKSQGYHDAADEQFAIGTIKFNTCTVAECDGHIVGFRLAYPSNKDNQVAAAYSGRPNTAFEKLMGFVCALERKSNVFARYQVDTVMQGHMLCVHSDFRGKGIARRLYEENMELAKQKGFPVYVCDCTSLFSARLCEKLGMEQTATMEFHEYCDENGKPYYSPEPPHDYARSYAKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJN5</accession>
    <name>T1GJN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02054675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJN5"/>
    <dbReference type="STRING" id="36166.T1GJN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003685-RA">
        <property type="protein sequence ID" value="MESCA003685-PA"/>
        <property type="gene ID" value="MESCA003685"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818002_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035060">
        <property type="term" value="C:brahma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071565">
        <property type="term" value="C:nBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021906">
        <property type="entry name" value="BAF250/Osa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12656">
        <property type="entry name" value="BRG-1 ASSOCIATED FACTOR 250 BAF250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12656:SF5">
        <property type="entry name" value="TRITHORAX GROUP PROTEIN OSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARID" evidence="2">
        <location>
            <begin position="37"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003685-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16621" checksum="6A5D586B64EAF46E" modified="2013-10-16" version="1">MDVISGFNSHVQQETFRPTPSNKKSDSLGKLYEMDEMPERRPWLDKLLGFMEERKTPITACPTISKTPLDLYRLYLYVKERGGFVEKLYVVNIRLSFLNGGDDHQLSYRNHHMKSLKFFCVNVVVPPRNPYKTPNSLRLKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK96</accession>
    <name>T1GK96_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK96"/>
    <dbReference type="STRING" id="36166.T1GK96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003915-RA">
        <property type="protein sequence ID" value="MESCA003915-PA"/>
        <property type="gene ID" value="MESCA003915"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF135">
        <property type="entry name" value="CLIP DOMAIN-CONTAINING SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="24"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003915-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="22371" checksum="2D21094B93C94163" modified="2013-10-16" version="1">MVFKFSNGVTLEDHRQATGKLIIVLTSAHCTQRDGHSPDFVRFISSNLSLLNGFDFKIISIIVHPDYDPNEIQHDIAILVLDKYNPLPPACLFQTLDPPFREVLALGYGATEFGGKSSTALHRVELDLYTNSECQNFYTQETIPITLTDYEICAGDIFGERDTCQGDSGGPIIVKIQRQLERIPYIIGLTSFGDQCASGKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKD4</accession>
    <name>T1GKD4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003957-RA">
        <property type="protein sequence ID" value="MESCA003957-PA"/>
        <property type="gene ID" value="MESCA003957"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323049_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003957-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11205" checksum="B327106E15E95C5F" modified="2013-10-16" version="1">MGTMSMGFYEYTGDDGRVYRVNYNSGKGGFMPMGDHIPTIPPQIKRALEYTARKEAERAAAKAALATPAPILAEEQVQEYVESPEESESRYRPIHQRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKI6</accession>
    <name>T1GKI6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts (via cytoplasmic region) with ILK.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02095585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKI6"/>
    <dbReference type="STRING" id="36166.T1GKI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004012-RA">
        <property type="protein sequence ID" value="MESCA004012-PA"/>
        <property type="gene ID" value="MESCA004012"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076656_0_1_1"/>
    <dbReference type="OMA" id="YHDEKAV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="143"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="24446" checksum="4CC08E3DEA92B549" modified="2013-10-16" version="1">MVFYFTSNVVSPPFDMYMGLDKYENEDLIRWGWPEDVWFHVDNYSSAHVYLRLKKNQTIDDIPQSVIDDAAQLVKANSIQGNKINNIEVVYTMWSNLKKTPSMEAGQVAYHKDKEVRKVRVEKRINEVVNRLNRTKREEFPDLRAQREERDAEERQEQKKLLRQQKDKEKEEMKRKAEEAELRSYTKTLRFSELPEDIKGTLLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL12</accession>
    <name>T1GL12_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02118505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004197-RA">
        <property type="protein sequence ID" value="MESCA004197-PA"/>
        <property type="gene ID" value="MESCA004197"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006523_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004197-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14591" checksum="DEF7AE728E8B5279" modified="2013-10-16" version="1">MVMQWLLNPFASEFNPNDGFNPIDGINKKFHHPWILFFSSKEGYKYYYYNNNIYVLLYAMYITSTCKIMNRSIHDCTLKEELMHMRPPPPESLGPIFTIGDYDLRFEALRLKGGYCLQTDVCLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL25</accession>
    <name>T1GL25_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL25"/>
    <dbReference type="STRING" id="36166.T1GL25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004211-RA">
        <property type="protein sequence ID" value="MESCA004211-PA"/>
        <property type="gene ID" value="MESCA004211"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009659_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004211-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14202" checksum="35162B67B452A14F" modified="2013-10-16" version="1">SRQSRKERNEGRIAKERERERAHKRKININKFSSYTFFMSEDNAGACYLSTQEYSCEICGMAGLSDDLMREHTRTHHVEGSAQCPFCGFSGVVPAELLLHVNQAHLDYLTPENELMSFIDDQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL78</accession>
    <name>T1GL78_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL78"/>
    <dbReference type="STRING" id="36166.T1GL78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004273-RA">
        <property type="protein sequence ID" value="MESCA004273-PA"/>
        <property type="gene ID" value="MESCA004273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_492847_0_0_1"/>
    <dbReference type="OMA" id="MQFNQID"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="553" mass="63052" checksum="6FB29BFC4FE15D50" modified="2013-10-16" version="1">MIASLSFISIPERPFRDMSSVVILSFSDNELDEIPKHALSHFPNVSTLDFAKGKINFISRDDFKKNQDLRHLILVNNNISRIDCDAFPNKLRQIHLAHNRINSLNGTLKELESLESLFVNNNNISSLEGELPNSPFLRFILAQHNELEKVPKLATYPEMLAFNQIESINSDEFKDCRSLEDIDISNNNLKSTNGSMKSVMELRISNYSYNKIKAFNLDEIKGLKFLKTLDLSHNEIEYLYQEDYSEIHDKFHFTFEFHLEYNNLKSLNSAFKGINSLRSIILTHNKLEILSSEDFSGLPNLETLDVSHNVIVTLEQLAMTFLPNMKTLRASDNNITSLDKDFHNMPTLCQAHLNNNQITSVSYELVSNTQCTNLGVAGKLEVWLENNPIMCDSNTYTELCSQLARRNSRLRGKSQCYENDMEVCLDDPIDIINVENSNSYKIHHIPIMKAGGTPSTTKQRIATLTPTPQTIANPNQMMATIQTMYAQAMTKPLIIDTNNNNNNIIFNNNTLWKPEVITQPTPLPEPTILTVQDPMMSKRNTKEDNNDIKLDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLF1</accession>
    <name>T1GLF1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLF1"/>
    <dbReference type="STRING" id="36166.T1GLF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004352-RA">
        <property type="protein sequence ID" value="MESCA004352-PA"/>
        <property type="gene ID" value="MESCA004352"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2352600_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11009" checksum="B922B7D85CE8DE4A" modified="2013-10-16" version="1">MSWIYYGVDRPLSCYCVKAENRLEVDAFRFSDPCLINPSDNESKMSGLMQDGAQQVQGTAADTDSNSNNKFFLALMEQINLLHETNSKICRNLHETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLU2</accession>
    <name>T1GLU2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004506-RA">
        <property type="protein sequence ID" value="MESCA004506-PA"/>
        <property type="gene ID" value="MESCA004506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169769_0_0_1"/>
    <dbReference type="OMA" id="GHDPDKY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051738">
        <property type="entry name" value="SAF_Modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15683:SF8">
        <property type="entry name" value="SCAFFOLD ATTACHMENT FACTOR B, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15683">
        <property type="entry name" value="SCAFFOLD ATTACHMENT FACTOR B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SAP" evidence="5">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9392" checksum="44E1D040790357C7" modified="2013-10-16" version="1">MDAANDKELKLLSDLRVCDLKVELEKRNLDTTGNKTVLVERLSAELERLGKDPKTFNFNETSDESTEETSSGKEKDIQEESES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW1</accession>
    <name>T1GLW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02038195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLW1"/>
    <dbReference type="STRING" id="36166.T1GLW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004525-RA">
        <property type="protein sequence ID" value="MESCA004525-PA"/>
        <property type="gene ID" value="MESCA004525"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004313_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00136">
        <property type="entry name" value="PDZ_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043545">
        <property type="entry name" value="GRIP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46227:SF2">
        <property type="entry name" value="FI03335P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46227">
        <property type="entry name" value="GLUTAMATE RECEPTOR-INTERACTING PROTEIN GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="149"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="233"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="424"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="539"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004525-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="624" mass="67950" checksum="A0890151AC930C0E" modified="2013-10-16" version="1">MGPLLIEIERPMNDKLGLVLSHYSPAAAATQLGPLDPTKLDEVSSAEMGVYISSILPASIADRHRTFVIRPEEVMALLDTNSGRGYTQLQIMPSHTLARRGYYMGNPKYSCGTLESRKCRPKKFVRKSSLPLEFSQSNLIGVCRSETVLVALDCSQGSGIILGAQSANGKGILIAQIIPDSVAERTGCIQKGDRIISVNKMYNLASQRYGNFLEMLDQSPVQFIKTQAGGVFNVKLAKTGKADLGITVINSNNGAFIISEVKPGSPAHRTGSLRAGDILLAVDSHPLQHFNLDVLLKDNKNDFTTLTIKRDSIPEFYFDGQQKAMGSLCFNDLYGNNYNQMKATTPEYFQNMQVPMRQKTPKQMTPSGGRPPFAEEISENVAENIYNEMSSYDTDYLSDKFTTLSCRIPPPMENKNYGAGHTTIVNIQLEPNGGPLGITLAGSEEISKPITISGVTPGGVADKTGEIFVGDSLVAINGEPVKGIPLSKAKKMLQNLTNDVIHLKLIRNFDYSSEGNGNVAQGQTLYAKVQRPRPRSPSFTDLQSNGSGSDLTMKFRILNVSLGVYINRMRPGGPAAMSGLLKPFDRILQVNGTKTLDFDCCLTVPLIAAAGDQIELVIQRLITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLZ6</accession>
    <name>T1GLZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Timeless N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02188421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLZ6"/>
    <dbReference type="STRING" id="36166.T1GLZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004563-RA">
        <property type="protein sequence ID" value="MESCA004563-PA"/>
        <property type="gene ID" value="MESCA004563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007646_0_0_1"/>
    <dbReference type="OMA" id="RMHLVIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009649">
        <property type="term" value="P:entrainment of circadian clock"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007725">
        <property type="entry name" value="TIMELESS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF5">
        <property type="entry name" value="PROTEIN TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05029">
        <property type="entry name" value="TIMELESS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="5">
        <location>
            <begin position="25"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Timeless C-terminal" evidence="6">
        <location>
            <begin position="726"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="186"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="929" mass="105557" checksum="F8380F2CB45A0A66" modified="2013-10-16" version="1">MDWLLSSPQLYSVFSSLGKQQGDSYIVSPDALNFLEEIHCKLTYEDQTLRTFRRAIGIAENVRVDLIPLLVNAKDPSVIDASIRIIVNLTVPVECLFSVEVMQRTEVGRQTIFEINKLLLSSKEAFTDGKATKSGIDKLLLYLMTCPKRTCWSVTMVQLIAVLYKDQHINTLQKLLNIWFEEAISESSEDNESNTSPPKQGSGDSSPMLTLSDPTSDSSDNGGGRKNDNSDEDTRQASPTEATLQEVCRKGQEYQDAMEATEEQKIENTPEFREPPSPSSLKTKSPHGGNMKLSVGKSTPQKRECPSSQSELSDCGYGTQVEPHESISTSSNDDEGPTKCPVHQKPPTNQRTKQRTVLTEQQRKDIRKKKLVKRSRSNLINMKGLIQHIPTDEDITNILKEFTVDFLLKGYDYLLEIDLENLRSVLSFKIISYIVYEGVSICEQVELNSQHKGNDLKPYLRRMHLVITAIREFLQALESYKNVSDLTEDDTIHLTTLRSKICSSTDLPNLFILLLRRFNPSMHSKQYLLDLIITNHLLLMVLDDRNCSFNLKDHLSQFATVEIMDNYGLILEDFLENGSYVNDCLFTMMHHVGGDLNRVTTLFRPSILKSFTRIWETEYEVIDDWSDLIEYVINKFMNTQQKESLSIAPELNDIASEAIATNAGSWGQEDMNNLYWYYVQSKKADDVVDATYQRNLLQNPSVADSGIDLKDSQECKPSDDIQILKELIIKENKRNILQWLQHVLLECIYAKLMIKEREDPFTPPPSASILRPKRGFIIEPVAYHCISKNKSIPIVPWNSDHSAMMQYHPFVLLLHKLGFHLPADSGSVYPRVPEFWTADLMYNIALKLGSMDKGTLKFEISNDENFQQISGDSLGKDESSKSSSDSINNFSLQVQKSQMGIRYIPDPSSKPSVPNWLQLVMKSKSLSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM12</accession>
    <name>T1GM12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02117644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM12"/>
    <dbReference type="STRING" id="36166.T1GM12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004580-RA">
        <property type="protein sequence ID" value="MESCA004580-PA"/>
        <property type="gene ID" value="MESCA004580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111641_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14839" checksum="EE1F53569F6B8229" modified="2013-10-16" version="1">MPFATSKFNFQNKTFYLIGVMGTSLGRAVVAHSQSFELTVAAFAWIGMNKAFRIIFTQLIIPGYVPLNRLPAAAGLQLLITGIFTLGCGPIVGMIKDATNYTVTIHFLNGICFLAGAAWVIEDLFFKPKKQMDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM55</accession>
    <name>T1GM55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02132330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM55"/>
    <dbReference type="STRING" id="36166.T1GM55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004623-RA">
        <property type="protein sequence ID" value="MESCA004623-PA"/>
        <property type="gene ID" value="MESCA004623"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112964_2_1_1"/>
    <dbReference type="OMA" id="QYYELYR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="8">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004623-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12387" checksum="07B1322CAD598413" modified="2013-10-16" version="1">MSYQLYRNTTLGNTLQESLDEFIQYGQITPQLAFKVLIQFDKCINNALNQKVKARVTFKANKLNTYRFCDNVWTLMLNDVEFREVHEFEKVDKVKIVACDGKSSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMP7</accession>
    <name>T1GMP7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor Tu, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02141778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02141779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMP7"/>
    <dbReference type="STRING" id="36166.T1GMP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004825-RA">
        <property type="protein sequence ID" value="MESCA004825-PA"/>
        <property type="gene ID" value="MESCA004825"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_880795_0_0_1"/>
    <dbReference type="OMA" id="MECELIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000001">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF36">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004825-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="316" mass="35127" checksum="3CED462A36C74B53" modified="2013-10-16" version="1">MPQTREHLLLAKQIGINHIVVFINKVDAADQEMVELVEMEIRELMTEMGYDGDNVPIVKGSALSALEGKNPEIGAEAIKALLKEVDTFIPTPARELDKPFLLPVENVYSIPGRGTVVTGRLERGVLKKGQECEFIGYNKVLKSTVTGVEMFHQILEEAQAGDQLGALVRGVKRDDIKRGMVMCKPGSVKALDQVEAQVYILNKDEGGRTKPFMRCKNTDDCGGGRHFDSDMDGKMIPRKRGYDQQEIKTEMGEKQQPYNFITQEVIEASIQCMLAQAEDCKKSGKSVEETELRVIEEFGRCLVEIISFSIKTSGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN19</accession>
    <name>T1GN19_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-conjugating enzyme E2-18 kDa</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02054651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN19"/>
    <dbReference type="STRING" id="36166.T1GN19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004958-RA">
        <property type="protein sequence ID" value="MESCA004958-PA"/>
        <property type="gene ID" value="MESCA004958"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_3_1"/>
    <dbReference type="OMA" id="ADLHTWH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23801">
        <property type="entry name" value="UBCc_UBE2L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000011">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004958-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17978" checksum="1FE06BAC71FD8B01" modified="2013-10-16" version="1">MTNPHRRLQKELSDLKAAGLKSFRDITVHDDNILFWTGLILPENPPYNKGAFRIDINFPAEYPFKPPRITFRTKIYHPNIDEKGQVCLPIISTENWKPATKTDQVIQALIALVNDPEPEHPLRADIAEEFLKDKKKFNKNAEEFTKKNSEKRPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN40</accession>
    <name>T1GN40_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN40"/>
    <dbReference type="STRING" id="36166.T1GN40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004979-RA">
        <property type="protein sequence ID" value="MESCA004979-PA"/>
        <property type="gene ID" value="MESCA004979"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_264758_0_0_1"/>
    <dbReference type="OMA" id="IWCHICN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="882"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="939"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="967"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004979-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1028" mass="117710" checksum="98E009C120D649F2" modified="2013-10-16" version="1">MNIHSGEPIPPGFENDLSRSAEIRPAIDRYKSGALIGLEYTVEVYTPKEKEPFYMCMLCEKRGDPRTIFAHWVSLNHRNKYLEKYFPTVYKELNSTRSSNLPGAIQVVIEAIEKRYGRLYPFKSRLYLYDIEDSEHFNERQGDTFVSFVDRKLLDPSGSNDKTSSSFNKDPSPPIVKPPVKRPGPIKVTLNTKSLPNESLDAISSEEENGGKKKRREQSPAYRVTPRVHRSPPFDEVTAKKDINNSKDKNPFPSPRELSLQAGQIAQEKYKWEKYRCSAELAEKDLLKKLKEFEKNPEKHPMYPEEWKKFWNRRYKELQMQKKDPNNYDYKPEWIKFWTVRMKELHDKDLEKKKVEIKLKLGLPPDGEEKTDVLKSQYKIDNPKQKSDSVEQVIDISDEEDSFVDIKRRSVRNSRDRNSRDRNSREGISKDRSSRDRRGSRDRRDNTKDRDSRRKRRSFSRSLSPLSDDVAVIEERKEIVTDQGLNHVQFQEKERSEWERKYPPGPHPYPYPPFARAFPPLYRPPLGYPPYGAPYRPPYSKYPKYEDYEENSKSPASSEHKKKEAPPAQEEEPETEEPLTVVSVLRLLTALEDYLGSLGPKVVDMLGKALALEKIRSNSADDLLINEDNCVFFETIKEKLKGQLLANVLDDQLKIKAVKKAIKNIAGIIYQVSSKVSKNEQASVQNVPTDKAIAPKTAKKISDLPFERSVVSSKLAAALITEGKEDVSTEEMDKLIFLLILMTKISKGNDKRVNSKEICEFLKIQRSRSPTPPPVATKQVIDIFSKDEIESMKMVVNSKDSKPSRKEEPRFNDSVKDTSGASGLDCLTDSDLQTLLQNFKHLSSEEQHHLIAHLKKLESTDPLRVEKLRKYVNISDDEISSKKPSFEIDEDDDSRSINESPPPPPSCKTDSRKINISDDDEDEEDYNFEDVVKSITAKNLQPKQALNPPRPVASTSNAKPTPPISIADTQNLIANLMGSLQKTNPPMPQTPQQQPPPPSQPPAPQMKSHPNNPQILCHSTNKTLSNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN59</accession>
    <name>T1GN59_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005001-RA">
        <property type="protein sequence ID" value="MESCA005001-PA"/>
        <property type="gene ID" value="MESCA005001"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256786_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051944">
        <property type="entry name" value="BEACH_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108">
        <property type="entry name" value="BLUE CHEESE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108:SF4">
        <property type="entry name" value="BLUE CHEESE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005001-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11976" checksum="14ED0BF37F5FCE92" modified="2013-10-16" version="1">MRKKTMKNDLFYLHYPYRPEIDLPDNKFSDPKTDPHSVKLLTLVRTLNNTREENLVCLTILLSARDKAIVVSTNETLLTPSKSMGDWEPEGGDDNNARIWCPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNI6</accession>
    <name>T1GNI6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02165286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNI6"/>
    <dbReference type="STRING" id="36166.T1GNI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005140-RA">
        <property type="protein sequence ID" value="MESCA005140-PA"/>
        <property type="gene ID" value="MESCA005140"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1070749_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019427">
        <property type="entry name" value="7TM_GPCR_serpentine_rcpt_Srw"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053071">
        <property type="entry name" value="GPCR1-related_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47023">
        <property type="entry name" value="SEX PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47023:SF1">
        <property type="entry name" value="SEX PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10324">
        <property type="entry name" value="7TM_GPCR_Srw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005140-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="260" mass="30079" checksum="FA6CF93DA67CB761" modified="2013-10-16" version="1">MYDKYTNKYIFVCHAPMARTWCTMQRVKRCTFCILVAAFLHQVPRILDQKYNIIQIPWENSTAEVCHVETSSWVFDFGVDIYYMIFFMFRIFFVHLIPCALLVTLNLLLFRAMKKAQDRRKKLLHDRNKDCSKTRDANCTTLMLIVVVTVFLIVEIPIAVITSLHVMSSMGYLSFLAIQSTLRYTVECPGSSGLHLKSFSSRIQVQLVKNTPWSMVLEPVPMKQYYSMFGSQQFLFVVLTSNFGTQIHDLDAMMALDGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNI8</accession>
    <name>T1GNI8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Malic enzyme N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNI8"/>
    <dbReference type="STRING" id="36166.T1GNI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005142-RA">
        <property type="protein sequence ID" value="MESCA005142-PA"/>
        <property type="gene ID" value="MESCA005142"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011405_1_0_1"/>
    <dbReference type="OMA" id="PINIMIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000004">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF80">
        <property type="entry name" value="GH17657P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577507" description="Malic enzyme N-terminal domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="6">
        <location>
            <begin position="71"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005142-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="289" mass="32917" checksum="C3F2A63056B7087E" modified="2013-10-16" version="1">MSKSWWMRALWLIPPFTLGLAFTLEERQLLGIQGLLPAVVRSEEEQVKHCLILLDRLENDLDKYMYLNNLAERNERLFYKVLSSDVAKMMPLVYTPTVGLGCQKFSLVFQYPKGMYISINDKGHVYDVLRNWPETDVRAIVVTDGERILGLGDLGANGMGIPVGKLSLYTALAGIPPQQCLPVTLDVGTNTQSILDDPLYIGLRHKRVTGQEYDDFIQEFMEAVVRRYGQNCLIQFEDFGNANAFRLLEKKYMEGLEILRDRNEDFIEMSKGFVPISFACIIKMVTSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNX9</accession>
    <name>T1GNX9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho guanine nucleotide exchange factor 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNX9"/>
    <dbReference type="STRING" id="36166.T1GNX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005292-RA">
        <property type="protein sequence ID" value="MESCA005292-PA"/>
        <property type="gene ID" value="MESCA005292"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018788_0_0_1"/>
    <dbReference type="OMA" id="GMHEAHP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11877">
        <property type="entry name" value="SH3_PIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026:SF1">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="82"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="267"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005292-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="49985" checksum="B24FF1FCA444532C" modified="2013-10-16" version="1">MDADRRLIVQAEFNFRGGNNDELVFNKGDLITVTQKEEGGWWEGTLSDRTGWFPSNYVKEYKGPSPSSAPECILNPIELLEHRNLVLKDFIETEESHVAELKGLLENYLIPLKSTDILNEQEYNQLMATSIEIKDGNTRIGKTFLGKAGILKKVHEAYCTTHPKAILILDKYKESLEEFMEKQGASSPGILVLTAGLSKPFRRLQQYSSVLKEFERYMDIDHVDRGDTQRSIVVYNELCTTCTKIRRQKELELQIMTGPIRGWQEKTLIALGEIVHMDLVSFVKDGKDKYMVLFPQLILILSVSQRMSAFIFEEKIQVFGIKVNRMEDPETFEIITPTDEKILVKCKNTSEVDKWMEHLDTGESNGKISQQFQHKRSLSFNHYLSYNQFQNREKQKSIYQKPSERINFVDNDKRVKWTIANLKCISPLRPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP17</accession>
    <name>T1GP17_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02131246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP17"/>
    <dbReference type="STRING" id="36166.T1GP17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005332-RA">
        <property type="protein sequence ID" value="MESCA005332-PA"/>
        <property type="gene ID" value="MESCA005332"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061027_1_0_1"/>
    <dbReference type="OMA" id="PNYREYM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000026">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005332-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="28021" checksum="5D9AFB0BAF69AA45" modified="2013-10-16" version="1">MVKLSPELINQSMQCINPCRERELDLRGYKIPQIENLGATLDQFDTIDFSDNDLRKLDGFPYLPRLKCLLLNNNRLVRIDDLLHESIPNIESLIFTGNNIQELGDLEPLTHFKNLQTLCLLVNPVSTKPFYREYMAYRFPNLRLLDFRKIKQKHKKEAQEFFKSKKGKDLLKEISKKISKPSNDGRGGGIMRGASPEEIQKIREAIKKAKNLQEIERLTQILQTGQIPENFFNGNGVEMMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP81</accession>
    <name>T1GP81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02035086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP81"/>
    <dbReference type="STRING" id="36166.T1GP81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005401-RA">
        <property type="protein sequence ID" value="MESCA005401-PA"/>
        <property type="gene ID" value="MESCA005401"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1429539_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000043">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="3">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005401-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21742" checksum="C0791621E5D84822" modified="2013-10-16" version="1">MPRTHRARTRSMHCAGRSSRSISRRHRKRVQTYGKYIRKIFKRIYPDMSISKRAVSVMNSFMNHIFKLIAVEAGTLVHYKKQQTICSAEIKSAVLLLFPRILAQHAIHSGEKATRNLIYSMNIRKSTASKEVYEYISNVFIDFLLNRAIVIYCLSGVIKFGNSSEKVEVKFKIAQSQVYSLPPSGAISLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPV5</accession>
    <name>T1GPV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DnaJ homologue subfamily C GRV2/DNAJC13 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040069">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPV5"/>
    <dbReference type="STRING" id="36166.T1GPV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005649-RA">
        <property type="protein sequence ID" value="MESCA005649-PA"/>
        <property type="gene ID" value="MESCA005649"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161256_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000641">
        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044978">
        <property type="entry name" value="GRV2/DNAJC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045802">
        <property type="entry name" value="GRV2/DNAJC13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19432">
        <property type="entry name" value="RME-8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C GRV2/DNAJC13 N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005649-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12515" checksum="69E752B9570F7889" modified="2013-10-16" version="1">MIPLKDNVDLECYLVTKHSWKGKYKRILSVGSAGISTYNPEKFEVTNRWSYSDIISIAPSKNSSAYKYHWSGISLPTVLEVTACSLNQLDPTSNQLLTSYYFKDIEGLIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPZ4</accession>
    <name>T1GPZ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPZ4"/>
    <dbReference type="STRING" id="36166.T1GPZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005690-RA">
        <property type="protein sequence ID" value="MESCA005690-PA"/>
        <property type="gene ID" value="MESCA005690"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029344_0_0_1"/>
    <dbReference type="OMA" id="ATHTNIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF21">
        <property type="entry name" value="NA[+]_H[+] HYDROGEN ANTIPORTER 2, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="7">
        <location>
            <begin position="10"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005690-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13605" checksum="8ABBB670E790B858" modified="2013-10-16" version="1">MRIIFLFVGAQISGIIVTFIKLPDMLGMLFFGVFYTNVGLADFSGYNGLEAVLRDMALINIMLLAGLGLDPKAFKKLWFMILRLTLVPTIVEVAIIAVLGYFLLSMPWLWGILLGTKLQSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQV3</accession>
    <name>T1GQV3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006018-RA">
        <property type="protein sequence ID" value="MESCA006018-PA"/>
        <property type="gene ID" value="MESCA006018"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963424_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006018-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6766" checksum="48E1CEAF190B7495" modified="2013-10-16" version="1">MSLKSVFGERATAYMLVTSSLKIELNGTYLYRPPLRMQTRQLAEISRFRFQIVLRGGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRC5</accession>
    <name>T1GRC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRC5"/>
    <dbReference type="STRING" id="36166.T1GRC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006205-RA">
        <property type="protein sequence ID" value="MESCA006205-PA"/>
        <property type="gene ID" value="MESCA006205"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085834_2_0_1"/>
    <dbReference type="OMA" id="YSHITAV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905:SF1">
        <property type="entry name" value="AT07410P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905">
        <property type="entry name" value="N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21149" checksum="23D47D47EE901DFC" modified="2013-10-16" version="1">MCDSKVNFRVGTSEDKPKVAQFMKKFFWPGEPICKSENLVISDEINDEAVSILELGTCTIAEDEAGNLIGLRLAEPRVPEDVEKKKVENPQNTLDKLGKLVYDLAIGAKVFEKYNVDKAMYSSALVVDENLGYPIYVCDCTSAFSAKACANLGFTEIFEIVFKDYQDESGKPVLHPDSPHDRAVVYVKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRD8</accession>
    <name>T1GRD8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02002436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02002437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02002438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02002439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRD8"/>
    <dbReference type="STRING" id="36166.T1GRD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006218-RA">
        <property type="protein sequence ID" value="MESCA006218-PA"/>
        <property type="gene ID" value="MESCA006218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913897_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000004">
        <property type="entry name" value="2-amino-3-ketobutyrate coenzyme A ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="24"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="305" mass="34165" checksum="4113EBEAF7B8E71C" modified="2013-10-16" version="1">MSCNRVLCKTGRGTEEFHNILGRVDIINSTLGKALGGASGGYTTGPKELIDLLRQKSRPYLFSNSLPPPVVATGIKVMDMLMNSEELSQKVSSNTKRFREAMTKAGFTISGQDHPICPVMLGDARLASVFADKMLSRGIYVIGFSFPVVPHGKARIRVQISAAHTTEQIDRCIAAFIEVGKELKILSRKMEGREHAYRGETILILNRLGPQQELEAVPHGVQQRNHMSTGDDVIHTGKRGKKLPLRYLVCNHRQDNVDEEYKRAVVEKKKSYQKDIQQKARTFKETLRKKGWEHKEDVTPPTASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRE8</accession>
    <name>T1GRE8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023590">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006229-RA">
        <property type="protein sequence ID" value="MESCA006229-PA"/>
        <property type="gene ID" value="MESCA006229"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349070_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588484" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006229-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="11130" checksum="9DAF8D325291D0E3" modified="2013-10-16" version="1">MKVSSNFLSLHLHFYIQVVVGSSNIEAKEARAPWTRYNNNNLVRTLQRCRIYATSPYKTPLQRKYNIEAFKTTEKISTQATLRATGGCKADISAYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRK1</accession>
    <name>T1GRK1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRK1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006286-RA">
        <property type="protein sequence ID" value="MESCA006286-PA"/>
        <property type="gene ID" value="MESCA006286"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545214_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006286-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9343" checksum="C4AE9637282B78DC" modified="2013-10-16" version="1">MVLRISWRDQVSTKNSTKFCWKFGIVKRCLKNGVIASSALSIRKETNGAKCISIFPLRGTNMDSDQNRFRISSSNTPDTLKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS51</accession>
    <name>T1GS51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS51"/>
    <dbReference type="STRING" id="36166.T1GS51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006499-RA">
        <property type="protein sequence ID" value="MESCA006499-PA"/>
        <property type="gene ID" value="MESCA006499"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_4_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577586" description="Peptidase S1 domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="29"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006499-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="26629" checksum="5D36185C74CD3312" modified="2013-10-16" version="1">MVSKVALVALLAFVATAAATPLFSIQGRIAGGSDGNSGDVPYLASLRVDDVHVGSCTLISETQCLTSAHLIIEHGKTTIAASRLSVRVGSINIFAAGKIVGVKKVAAHEEYGNFINDLAIITFAEKLVKGDKISFIQVASEEPAQDTVINIAGWGSTEIGGSNSFRLQKGTGNAITAQDCEEKIGFGYEHILCVASPAGQGICNGDAGSPAVNNNVVYVLPVSQLVPVPPNSQMFTPILFNIRNGSLRTLSKFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS85</accession>
    <name>T1GS85_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166576">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006535-RA">
        <property type="protein sequence ID" value="MESCA006535-PA"/>
        <property type="gene ID" value="MESCA006535"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624832_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006535-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9205" checksum="A737FE6E8EE776DA" modified="2013-10-16" version="1">MNLLKRLSKKHALLLKETQHLTMDLMRVLELSGGLTFYYLTDDCFPITSLERIFKGHARKTMFSPRSCSTLFKQKLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSI9</accession>
    <name>T1GSI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF753 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006649-RA">
        <property type="protein sequence ID" value="MESCA006649-PA"/>
        <property type="gene ID" value="MESCA006649"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399492_0_0_1"/>
    <dbReference type="OMA" id="TCINIAD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577098" description="DUF753 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006649-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="20325" checksum="02B8FA7581E14E03" modified="2013-10-16" version="1">MKAAILLFLAFGVLQSSSALQCYTCINIADCAKKTLEVEMCGEKTTSPDSTVTAPTTTTTESISPDPATNSTGSSPTTTTISTTNSTPPVSTTENITVTTLLPTTTTDTPASTTLNTTDTPTPTTLNTTDIPGPTTLNTTDITVPTTLNSNTTDSTPEPTTPNTNTSTIETTTPPTNQPPPPEDPQISIARRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSV6</accession>
    <name>T1GSV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSV6"/>
    <dbReference type="STRING" id="36166.T1GSV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006768-RA">
        <property type="protein sequence ID" value="MESCA006768-PA"/>
        <property type="gene ID" value="MESCA006768"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1095338_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052224">
        <property type="entry name" value="THAP_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927">
        <property type="entry name" value="AGAP005574-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006768-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="29197" checksum="3D65453182A4DC55" modified="2013-10-16" version="1">MPSFCVVAGCPYKETHSESINFHKFPVAKDLLARWKQFAEKPDNWQPTRLSAICSNHFTLTNFRNVKNKYLKSSAIPTIKTPVVEFNADEATAKFLDQKRPREEMSVRKPVFIHSSDLFPEHRVQKKIKIPEKFKKEFPNISNQKVKIPLEYEDEGENMEETPQPSSKQEPSNDGSFYEQFLQNSGNSDVLNQNSQNYETIEVLAEDVMVQADSTENVGEEVQTAEIMEVTEDYLKKTRKSIHIVGFVGKFVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT41</accession>
    <name>T1GT41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT41"/>
    <dbReference type="STRING" id="36166.T1GT41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006861-RA">
        <property type="protein sequence ID" value="MESCA006861-PA"/>
        <property type="gene ID" value="MESCA006861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_5_1_1"/>
    <dbReference type="OMA" id="CHEVESD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="307"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="613" mass="69407" checksum="9A37BD67AA04DDA0" modified="2013-10-16" version="1">KEPSPSEDLDEDNDEGGTVKTAAQKKKEKKERQKREAALAKQKAKDQPPASKEPTPAPKVEPEPEPEPVEVEDKEKSEEPENDKKNKKKKGKDKKAAAEEKEAEKKDTKKKGMSAAMVAAMQEKLKLIKEEEERLRKEEEEKQRQEEERERQRLEAIRLEKERKEKKKQKEAEKKARLKAEGKLLTKKQKEDRARAQNMLEALKAQGLDIPDPVEKRPRPGTRIRNNKKKVGTQKSEEEVKQDSQEEVSKESTPSVDEKKKEEEIKDSWDAALMKMTILKKSLKKLLPHINPKKFLRFRTQASWSSFIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTLESINLLKQKKCPFIVALNKIDRLYDWQTFQRRDIRDVIKNQQANTQLEFQQRTKEVILQFAEQGLNAALFYENPDPRSYVSLVPTSAITGEGMGNLLYLIVESCQSLLPKRLMYSEELQAIVLEVKAIQGLGTTIDAILINGRLREGDTMIVAGTDGPIVTQIRSLLMPQPLKELRVKSAYVEHKEIMAAQGVKIAAKDLEKAIAGINLVVANNPDEVEIGREEVAGELKNALSHIKLKQLGVYVQASTLGSLEALLDFLGASKIPNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTD2</accession>
    <name>T1GTD2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Alpha-mannosidase</fullName>
            <ecNumber evidence="10">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02097228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTD2"/>
    <dbReference type="STRING" id="36166.T1GTD2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006962-RA">
        <property type="protein sequence ID" value="MESCA006962-PA"/>
        <property type="gene ID" value="MESCA006962"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004690_2_0_1"/>
    <dbReference type="OMA" id="QVMGIMQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000018">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017849788" description="Alpha-mannosidase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="11">
        <location>
            <begin position="362"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006962-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1009" mass="115489" checksum="6704604860A0AC22" modified="2013-10-16" version="1">MAALKYLLLCSLALSALANPTRNARTCGYKGCPKGEKGMINVHLVPHTHDDVGWLKTADQYYYGSRNGIQRAGVQYILDSVIQALIQDPNRKFIWVESEFFFKWWAEQTDELKEQVKILVEEGRLEFTGGAWSMNDEATVHYQSVIDQFAFGLKKLHDTFGECGRPRIGWQIDPFGHSREMASIFAQMGFDGQFFARLDWVDKGTRMKDKTAEIIWHGSPNLGSSSDLFTGALYNHYSAPPGFCFDTLCQDEPIIDSKSHVRNVELKVKHFLDYIQNLSTKYRTNHVMVPMGDDFNYQEANMNYKNMDKLIKYVNEMQIKNNSNVNVFYSTPACYLKSLNDAKITWPEKHEDFFPYSTDPHTYWVGYFSSRPNLKRFERDAKLSSKDSKYSENLDQLRDIMGVMQHHDAVTGTEKQHVSNDYEKLLTDGFVACEQNVRAALREFTKLENANFQSCLNLNISACAVSENMSKFVVTLYNPLSKPVSQSVRIPVHEGTYEVKDSSGKIVPSQIVPIPSSVTKLPFRDSTSLKELVFNANILKLDSFLIQRTSNVEETIKVENPTAKFVVKNSNVYLEFDDTGRLSLVRTNGVEVPVKQDLLYYDGAAGNNAIFANRSSGAYIFRPNGTIHQIGEKIELKVIDGPLVKEVQQKFNDWISQVIRIYEDKSYVEFEWLVGPIPIEDNIGKEVISRFSIFYTDSNGRDMLKRTRFEREDFEAIYDEQESSNYYPVTANIALEDSRIRMAVLNDRAQGGSSLSDGTLELMVHRRLLRDDAFGVGEALNETAYGKGVIARGKHYLMIGANNKQTKINERKLQLELLLPHWKIFSSDTAYKSKPNVNQAFSSLTSLPENVNVMTLEPWNDNEVLLRLENILEKPEGSNVTVNLSKICKQLRFIEIRETTLDGSMWLSDLKRLRFTEDNGVIDTTLQKSPYTPFFATSASNLKDFDIVITPMSIRTFIKKYKHRRNPFPPQQANGNIKRDIDNLKIKPHTICCRNNKKRKSFTKETNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ8</accession>
    <name>T1GTQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02018143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ8"/>
    <dbReference type="STRING" id="36166.T1GTQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007099-RA">
        <property type="protein sequence ID" value="MESCA007099-PA"/>
        <property type="gene ID" value="MESCA007099"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_0_3_1"/>
    <dbReference type="OMA" id="SDARFWQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000179">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577622" description="Lipase" evidence="4">
        <location>
            <begin position="21"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007099-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="390" mass="44848" checksum="DC8795BEE71F1D4A" modified="2013-10-16" version="1">MMKVYALLVISAYLVLFVEGAKDLVCQELQIHNYPCESHEVLTEDGYVVTLHRIPPRNLTYAKELKPFILMHGLIGSAADFILSGKQRSLGCMLHEKGYDVWLPNARGTTYSKRHMHFDSSSESFWNFTWHEIGYYDLPAAIDFISNKTGHQKLHYVAHSQGSTIFLVMLSERPEYNEKIISASLLAPVAFLANLESPPLRLMASESEKIETLLNHLGLHELFPSTALNQLGGHLFCGKGAPTQNLCILVTYLSVGFSDYEMDRSLFPKIFETTPAGISRKQFQHFGQLINSGKFQKFDYKSKEENYKRYGRKTPPEYNLRNIRVPLNLYYGNKDFLMAKQDVFRLATHLQNSKFTMTEIRGFNHIDLLYSKEAPRFIYKKILSDVQRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU09</accession>
    <name>T1GU09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02016773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU09"/>
    <dbReference type="STRING" id="36166.T1GU09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007208-RA">
        <property type="protein sequence ID" value="MESCA007208-PA"/>
        <property type="gene ID" value="MESCA007208"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521543_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007208-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19398" checksum="BF46072126926CA0" modified="2013-10-16" version="1">MNTNRAQKFKRECSATKQKECSDIRTSNITAAKAVSDAIRSRAQNMKALLEAAEKLLQKEIHLIAISDSFQCCAYKALNILTEMSTPIDQECIRQNPGPTISIICRGSNKLTLEEAVRYLHDANTLDGVNACCFRAFAEALEVIPLSLAENAGLNPIATVTEFRNRHANGDNTAGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUI3</accession>
    <name>T1GUI3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUI3"/>
    <dbReference type="STRING" id="36166.T1GUI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007397-RA">
        <property type="protein sequence ID" value="MESCA007397-PA"/>
        <property type="gene ID" value="MESCA007397"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778758_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007397-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7565" checksum="9782E4C493989735" modified="2025-10-08" version="2">MQGLVGAFGGGLSTLGGYNGLPSVLSGLDESMSDVGHRKKYPFELEKAIHNVMFIQHHMQRQDEFNASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUJ3</accession>
    <name>T1GUJ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUJ3"/>
    <dbReference type="STRING" id="36166.T1GUJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007407-RA">
        <property type="protein sequence ID" value="MESCA007407-PA"/>
        <property type="gene ID" value="MESCA007407"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838560_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051320">
        <property type="entry name" value="Viral_Replic_Matur_Polypro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064">
        <property type="entry name" value="POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064:SF37">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7469" checksum="8AD1C042F8FC65F8" modified="2013-10-16" version="1">MDELLKKVKVNKSKCRFLQHEVEYLVHLISAEGIRPGVEKLTDIKNASVPKDVSQLPSFVGMLTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB5</accession>
    <name>T1GVB5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEAT repeat-containing protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02053256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02053257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB5"/>
    <dbReference type="STRING" id="36166.T1GVB5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007706-RA">
        <property type="protein sequence ID" value="MESCA007706-PA"/>
        <property type="gene ID" value="MESCA007706"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_890196_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025283">
        <property type="entry name" value="DUF4042"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052107">
        <property type="entry name" value="HEAT6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366">
        <property type="entry name" value="MALARIA ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13251">
        <property type="entry name" value="DUF4042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4042" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007706-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="313" mass="35462" checksum="210885917AFDD82C" modified="2013-10-16" version="1">MILIMYEQLTHALANENSIPVLTQSLKCLSLLIQATPFHRMKTGFVPLFLQHIKSLVHHKDQSVQVASLMVMEFLVLSPGITKEISNLVEIPTMGKLSKHTEKLDEDLDEEYLSGEESEILPEEVAEPTPTNNNYMPWLLEVVVENFGVCVRHPELKRTTKQVSIRIESLQVLTALCKHFQMLKDHLSIIAIVLQKELYDPQDEVRIYTAKCLDTLAHEMSNYLEIAPNDIEDCLHFWRSMLPSVITIIQDPSTSHVIKSSLCDFFSNIGVNIFEKLDHSDHMKLVELLSGVSCSEEPTVKSASVRVLAVYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVC5</accession>
    <name>T1GVC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02059569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVC5"/>
    <dbReference type="STRING" id="36166.T1GVC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007717-RA">
        <property type="protein sequence ID" value="MESCA007717-PA"/>
        <property type="gene ID" value="MESCA007717"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1125645_0_0_1"/>
    <dbReference type="OMA" id="MELAMYF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007717-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="27049" checksum="7C329C77EE9E981E" modified="2013-10-16" version="1">MVTSMLPIALLPMLGILGSEDTCRSYFKDTTIVMMGGIIIAMAVEYSNLHYRLALLIIKRFTCYPRVLHLALMGITFCLSTTMSNTATAALMSPIVRAILENLNDEEVMKIYLEMEKNDSNENRRPTPQAIAFYLGVAYSATIGGLIFEVIMLSMFLTAFGSNVSIVDQSFGFLAEASLNVKKHPLILFYPAVLASSMAFHLPVSTPPNAIACGFGNIKTKDIIYAGIGPTLSAILLLEIFVFIGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVM5</accession>
    <name>T1GVM5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007836-RA">
        <property type="protein sequence ID" value="MESCA007836-PA"/>
        <property type="gene ID" value="MESCA007836"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123893_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007836-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="13166" checksum="95DC1FE0FA6813B5" modified="2013-10-16" version="1">MQAQLPKGNHEDIEEVRKKKKDIDEKVKSERSSVAEMEKLYEQNQSNLNTLINRRNELTIIGVLNRTSVGEHDSSFNLLCDFVVVRNLGSQVNEVHNLEIVMTYVELRSYSFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVN7</accession>
    <name>T1GVN7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVN7"/>
    <dbReference type="STRING" id="36166.T1GVN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007849-RA">
        <property type="protein sequence ID" value="MESCA007849-PA"/>
        <property type="gene ID" value="MESCA007849"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_720763_0_0_1"/>
    <dbReference type="OMA" id="ITIQQCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000017">
        <property type="entry name" value="Raw, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039470">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36300">
        <property type="entry name" value="RAW, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36300:SF1">
        <property type="entry name" value="RAW, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15891">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007849-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="384" mass="43788" checksum="014439EFC0B96335" modified="2013-10-16" version="1">NEIIEEINNYSLDSDSESTGLTFEQFCILAADFKRLRGSTNIIRANPKKHSLSKSSSFINDEIRVKNATNKNIFKRSSSSPADRLGPEVFLGGSCNPTTWRADVAIPILQKLGISFYNPQVSEWTPDLIELEQKAKEKAKVLFFVMDSQTRATAGAIEAAHIAAQNPRQLVLVLHPYKMNQQIFKETISIQEYYDLQRNQQILKEMVTRRGLFVLEDISSGLERTKEIVTGARESSSNVRTVLNVVWRAFTRVLNLKERSITIQQCEKALRSLGYTQNLLTTLNLTQVIFLSQQYKQKSQLKKMFTNKFQIFFEDFCIITAYFSILQQEVYDSGCTSPIKGMIAPHPPIYLANVQVVPFILPFATFLQLVIHSFFACMLTRSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVS8</accession>
    <name>T1GVS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007894-RA">
        <property type="protein sequence ID" value="MESCA007894-PA"/>
        <property type="gene ID" value="MESCA007894"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429576_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000479">
        <property type="entry name" value="CIMR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00878">
        <property type="entry name" value="CIMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MRH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007894-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10451" checksum="4295D71420E63A88" modified="2013-10-16" version="1">MKKYKSYGKSSNLTYDKEQGLVMEFNSDVQCGDNKNYSSTILFICNNGDGKPEVTIKGCRIIFEWRTKYACNPKPCEGVHSESGVRFMYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW49</accession>
    <name>T1GW49_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA008020-RA">
        <property type="protein sequence ID" value="MESCA008020-PA"/>
        <property type="gene ID" value="MESCA008020"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_948557_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008020-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="294" mass="33351" checksum="901F98068DEDBA21" modified="2013-10-16" version="1">NHYENPHFLKLNKDDSGLIDLKVLPNEKDIKRNVSTTNFVYNDPIDHISVCVENEEENDVIVKSQATKTLVNAAITTNSKSDNYSGESIVIRISADQDNENECIPPTYSIPSTISSLMNKKVQLKQQQHQQHQNITKESIDSALENLNTVSSVSINKSDLTYKIQKTSSKKISPPPKPPRKAFNAPSRSSSFSEFHFQVKPKVEKNSNSTRFLEESAEDEEHDYHSYTCEPENEIYNDVHLLSSDSTIPYPLRVKTNPFTMQKELYAVNLGXXXXXXXXXXXXXXXXXLIKTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWE4</accession>
    <name>T1GWE4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWE4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008118-RA">
        <property type="protein sequence ID" value="MESCA008118-PA"/>
        <property type="gene ID" value="MESCA008118"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161255_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008118-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12977" checksum="6929AA8771DEAD40" modified="2013-10-16" version="1">MKEIHDLLTSKEILQRFFKNLLANFLCLVPQYLEVLDFLQFEWQNVYNTTEDQVYLNDPQGSPSSETFLNTEDVLPKLIFQRCPSKVDDIPSVLIAQPSTAHYMPDPKERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX26</accession>
    <name>T1GX26_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX26"/>
    <dbReference type="STRING" id="36166.T1GX26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008364-RA">
        <property type="protein sequence ID" value="MESCA008364-PA"/>
        <property type="gene ID" value="MESCA008364"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645104_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008364-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8003" checksum="4102072E09B1B0D4" modified="2013-10-16" version="1">SFKILDKVASIGANTLNFTDLGSPYEGPPSTPQSGMDAPDAPHTPKYMDGGSTTATVPGKSAFVELQQHGENVLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXY0</accession>
    <name>T1GXY0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Translocator protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXY0"/>
    <dbReference type="STRING" id="36166.T1GXY0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008693-RA">
        <property type="protein sequence ID" value="MESCA008693-PA"/>
        <property type="gene ID" value="MESCA008693"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1557046_0_0_1"/>
    <dbReference type="OMA" id="KWIFVES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033013">
        <property type="term" value="P:tetrapyrrole metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="entry name" value="TspO/MBR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057:SF0">
        <property type="entry name" value="TRANSLOCATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008693-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19640" checksum="B09A69197BEE7B1D" modified="2013-10-16" version="1">MKTKIAVRFYNLPGKHANPNFKNMTGVLLPSLTGIRVHSMNKNKLTELQQLKFLKQPAISIPWSVEKYIWPALNSSIGYASYLVYKEGGGLKGPAAIPLLSYGLHLTVNAFWTNEPLRWSIFQMFVSTSTAAVTGCQFFDVSKPAGYIFLPYVTWLTYYIYYLMYLHRINLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXY3</accession>
    <name>T1GXY3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02113252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXY3"/>
    <dbReference type="STRING" id="36166.T1GXY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008696-RA">
        <property type="protein sequence ID" value="MESCA008696-PA"/>
        <property type="gene ID" value="MESCA008696"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2139698_0_0_1"/>
    <dbReference type="OMA" id="HETNIMN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008696-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="13104" checksum="4673D20D939F44F8" modified="2013-10-16" version="1">MLDFKCRQDDLWVVTIPKCGTTWMQETAWLVQNDFDFDKANSILLTERSPFVEIEGLQDGICKSFKISDDLPSPRLLKSHLPASFLPKEIWQKRSKIIYVARNVKDTVVSFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYQ3</accession>
    <name>T1GYQ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytosol aminopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02146811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYQ3"/>
    <dbReference type="STRING" id="36166.T1GYQ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008990-RA">
        <property type="protein sequence ID" value="MESCA008990-PA"/>
        <property type="gene ID" value="MESCA008990"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009658_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF48">
        <property type="entry name" value="DIPEPTIDASE B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008990-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13526" checksum="EC4157B33499F8AF" modified="2013-10-16" version="1">MITARSGVRLRVGNTDAEGRMAMVDSLCKMRERVEKENLPNAHLFTVATLTGHACIAAGDGYSICIDNSVARNEGHCYKLQEVGNAVGDPFEVSYLRKDDFDFNCGKVLGEDILQSNNAPSSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZA0</accession>
    <name>T1GZA0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZA0"/>
    <dbReference type="STRING" id="36166.T1GZA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009198-RA">
        <property type="protein sequence ID" value="MESCA009198-PA"/>
        <property type="gene ID" value="MESCA009198"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047174_3_0_1"/>
    <dbReference type="OMA" id="LIANIMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009198-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="237" mass="26400" checksum="C2CE88BAAC85A292" modified="2013-10-16" version="1">MVGLGCLHSARVMQNLLLKSVLRWPMALFDTTPVGRILNRFSNDINTMDNALPELLLLLVSQIFTVIGTIVVISISSPMFLVVVIPIFILYYFIQRFYVATTRQLTRLESITRSPIYSHFGESISGVVSIRAYNCQKSFIEENDGFVDRNQTCKYPSVIAARWLATRLEMVSNLIVLFASLFAILGQQENAALVAPLQILVSLYLLWGVLGPSVLAGLAVMLVMIPINGWMANATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZK3</accession>
    <name>T1GZK3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009306-RA">
        <property type="protein sequence ID" value="MESCA009306-PA"/>
        <property type="gene ID" value="MESCA009306"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212953_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009306-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12428" checksum="2FC019685CEF90DE" modified="2013-10-16" version="1">MIKNGFGVNANKTELVIGYLRLLLVDLNLEELTKRRDLRLTRKAQEKVIGLDNKQPERDLIRCIKYEETRNGRTKGSNTDEAKEHQLTLQFLLRLPNKNLEKPAKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZN6</accession>
    <name>T1GZN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009340-RA">
        <property type="protein sequence ID" value="MESCA009340-PA"/>
        <property type="gene ID" value="MESCA009340"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560870_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577854" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009340-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9256" checksum="B7FC72F76E843A9D" modified="2013-10-16" version="1">MQTGFFTANHTCPLMMVALVRALHGRGTNLLHEVACPGSLFYIRNYPDVEWIVQECSPIRNSGTRIVSSPVISRILQLHYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H028</accession>
    <name>T1H028_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H028"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009492-RA">
        <property type="protein sequence ID" value="MESCA009492-PA"/>
        <property type="gene ID" value="MESCA009492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925225_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7222" checksum="45B5EB7E69509528" modified="2013-10-16" version="1">MSPLDENLPVLLKKISIGFSWEAQSKSVNENFDLRNKIQTKKNLQKLCERKRKKYIICLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H092</accession>
    <name>T1H092_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TTI1 N-terminal TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388128">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H092"/>
    <dbReference type="STRING" id="36166.T1H092"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009565-RA">
        <property type="protein sequence ID" value="MESCA009565-PA"/>
        <property type="gene ID" value="MESCA009565"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873111_0_0_1"/>
    <dbReference type="OMA" id="FVCINIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="1">
        <location>
            <begin position="3"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009565-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="36832" checksum="BF18B7AA8DA93BDC" modified="2013-10-16" version="1">RKDTLNTSTLQQIRNIIKNHSIRNVNELKVLLSISLREIKINESSSEKSEEHIEAGIDIIFNSLNNSYSDVIEEFYKEESIPILSNICLEFVKIIENHKKKKLKEKTVQCLMLLYFVDSSCDFDDKLIRNQAANCLYLFIPKIIKILVQIATGDAVLGDQLKTVSLEAFSKIICLVFEDYNITSTESYPTFEDFLKIIGPKKQNDVTINDEVLNIKKNMKTIRNRSKEWLEAASTKLTPLFKKLIPLSVSSVTTIRKEMCNMCQQLLLNCFENLNINTPYLLESLIVLSEDEDNDIKLRVLEICRKLSFVSRLIIMKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0C3</accession>
    <name>T1H0C3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02054515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0C3"/>
    <dbReference type="STRING" id="36166.T1H0C3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009598-RA">
        <property type="protein sequence ID" value="MESCA009598-PA"/>
        <property type="gene ID" value="MESCA009598"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962099_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009598-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="13952" checksum="9238F512CABE226E" modified="2013-10-16" version="1">MAHPFHQDMLVDGSNMLSPCHPEIGTKATAAGLGINDGNIVLGGFEFPQSNIDGDTTFTFSLQFVQNPGVFERSFAHFLSFLLEFLDGTLVNTSAFVDQMSGGGRFTRIDVSNDDDVNMNLFLTHGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0G5</accession>
    <name>T1H0G5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113070">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0G5"/>
    <dbReference type="STRING" id="36166.T1H0G5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009643-RA">
        <property type="protein sequence ID" value="MESCA009643-PA"/>
        <property type="gene ID" value="MESCA009643"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313159_0_0_1"/>
    <dbReference type="OMA" id="HEIDTAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031151">
        <property type="term" value="F:histone H3K79 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009643-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="10960" checksum="86432F29C5AD73AA" modified="2013-10-16" version="1">MAQPAIKDLILRSPAGSSEVITFSWPLQFGSGADKHDNGLDIIETIKYVCNDIPGIKSAFEETIFHEIDTACFKTMTNLVDKFNKAVDSIVNLEKGTSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H149</accession>
    <name>T1H149_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RNA helicase aquarius</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Intron-binding protein of 160 kDa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in pre-mRNA splicing as component of the spliceosome. Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19. Within the spliceosome, interacts with SNRPA1, SF3B1, SF3B3, SF3A1 and SF3A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the CWF11 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02031316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02031317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H149"/>
    <dbReference type="STRING" id="36166.T1H149"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009902-RA">
        <property type="protein sequence ID" value="MESCA009902-PA"/>
        <property type="gene ID" value="MESCA009902"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043993_0_0_1"/>
    <dbReference type="OMA" id="KWNERSQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000396">
        <property type="entry name" value="RNA helicase aquarius"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF5">
        <property type="entry name" value="RNA HELICASE AQUARIUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="19">
        <location>
            <begin position="23"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009902-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="32214" checksum="DDFBCE2D5D503DDF" modified="2013-10-16" version="1">MIGDHHQLPPVIKNMAFQKFSNMEQSLFTRLVRLGVPTVDLDGQGRARSSICTLYKWRYKTLDDLSHILNNEEYKRANAGFLHDYQLINVEDFKGVGESEPSPFFYQNLAEAEYIVAVYMYMRLLGYSNEKISILTTYNGQKHLIRDVVKMRCGSNPLIGWPHKITTVDKYQGQQNDFILISLVKTKAVGHIRDVRRLVVAMSRARLGLYVFGRARLFKNCFELQHTFNLLTSRPTTLQLVENETYPANRPISENVKNFKNISNMTEMAEYVSKIYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1I3</accession>
    <name>T1H1I3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1I3"/>
    <dbReference type="STRING" id="36166.T1H1I3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010045-RA">
        <property type="protein sequence ID" value="MESCA010045-PA"/>
        <property type="gene ID" value="MESCA010045"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645097_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010045-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8592" checksum="80CB4DB955A39066" modified="2013-10-16" version="1">WCKEKSLINSIKNSNGSSSVAKDRLEARTKYRAPGLKTKRSMTRDKYSSPSSEVTKSTPKLNNNMSVSCTLPKAEHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1L4</accession>
    <name>T1H1L4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1L4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010077-RA">
        <property type="protein sequence ID" value="MESCA010077-PA"/>
        <property type="gene ID" value="MESCA010077"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873114_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010077-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="35654" checksum="81ACEDB6E75E6E22" modified="2013-10-16" version="1">EKQTSASGASSTSAPVSPGPYSLDFLHQVGVQMTAGAVNQQLSLPQSHPHHLQVLAQANSSGNRAYNSPSISPLRHHHPSYLSLRHHRPYFNQDNSQMVLSDGTILSNNSNGGKSNNFEIPILYHHHPQHHFPPHRNSGIMNSPIAAESFPPFHHFHHQPASSHNYQNFLNPQINQQNPHYQQYNGNQGNNGGNLSRSNSSHMIQQKKSWNSWKSKNHHHHHHHHNNSQKNRANSSDHHSNSSCSSSSSTSSATSRNNDSNSKGNQNVRTLTYVSTDYVNSRSPTPSPKSISPIFQGSKRLPQNNPQLPHRLPYISWTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2I0</accession>
    <name>T1H2I0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02143843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2I0"/>
    <dbReference type="STRING" id="36166.T1H2I0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010427-RA">
        <property type="protein sequence ID" value="MESCA010427-PA"/>
        <property type="gene ID" value="MESCA010427"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052289_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010427-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13599" checksum="D58EB7B308AC9926" modified="2013-10-16" version="1">MKAICDLKTNSNGFQEGDLVILYNPKRKKGLCPKVQRNWKGPYKVLKRINDVVFRNHGMVLNHISLLVGNLGLVHVLCYNVVSSSGTNNGKRVKTGYGVFVFFLVYRSFGSWYSSRKGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2V1</accession>
    <name>T1H2V1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2V1"/>
    <dbReference type="STRING" id="36166.T1H2V1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010557-RA">
        <property type="protein sequence ID" value="MESCA010557-PA"/>
        <property type="gene ID" value="MESCA010557"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_0_2_1"/>
    <dbReference type="OMA" id="WHPAQSI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF4">
        <property type="entry name" value="RE04130P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010557-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="238" mass="26297" checksum="73668EFA7DFDF9F0" modified="2013-10-16" version="1">MLNSQKLTSKTPRQILEMTTAEKLSFLNSFDLVISDLDGVLWTEAGIIPNAGDGFNALKLGGKKVIFVTNNSVRSPKDYISKFSDAGVDFNPEEMVHPLTSIIDYLNITKFEGPIYCIANEVFKKAIKDAGFNVIEDQSKVFPESFTELGKYIFDKEKPNAVIVDVDFNLSGPKLMRAHQYLTRNPDCKYIVGATDKILPIRPGVQVMGPGCFQDALTQAVNRTPIALGKPGFELSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H304</accession>
    <name>T1H304_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H304"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010613-RA">
        <property type="protein sequence ID" value="MESCA010613-PA"/>
        <property type="gene ID" value="MESCA010613"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608770_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8994" checksum="4484394DD1FD2C07" modified="2013-10-16" version="1">MSADKSCQGRTKVIMNYWVRSSQLETMTSSSESWTLTYADAAANLWRCSIHQCRMADQVQLRTVRKKTFALGRACKKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H311</accession>
    <name>T1H311_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384752">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H311"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010620-RA">
        <property type="protein sequence ID" value="MESCA010620-PA"/>
        <property type="gene ID" value="MESCA010620"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929415_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010620-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6812" checksum="4630303FBCDA5E8B" modified="2013-10-16" version="1">RAVQQYKFDLPEDKAKTILTKCIADNKKEGASPFDYIVPVQKCLEASEIGDKLKAALSKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H327</accession>
    <name>T1H327_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379171">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379172">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H327"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010638-RA">
        <property type="protein sequence ID" value="MESCA010638-PA"/>
        <property type="gene ID" value="MESCA010638"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530026_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010638-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9608" checksum="9C22A196A69B43DD" modified="2013-10-16" version="1">MLLTDCEAIKKLPFMEYTNAKPSVLIGLNHSFLGCALETVEDEIRMGYKLGWSLQSWFKSEIFKFIENCGTKTVPNNQYKGNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H337</accession>
    <name>T1H337_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein heavy chain hydrolytic ATP-binding dynein motor region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H337"/>
    <dbReference type="STRING" id="36166.T1H337"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010649-RA">
        <property type="protein sequence ID" value="MESCA010649-PA"/>
        <property type="gene ID" value="MESCA010649"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041873_2_2_1"/>
    <dbReference type="OMA" id="MFRCYIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF1">
        <property type="entry name" value="DYNEINS HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010649-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="14983" checksum="F5E7D5C870D780DC" modified="2013-10-16" version="1">GLAQSGAWACFDEFNRIELEVLSVVAQQILTIQRAIARKVVKFFFEDTMLKLDPTCTSFITMNPGYAGRTELPDNLKVLFRTVAMMVPDYAMIGEITLYSNGFDEARPLAQKIVHTYKLCSEQLSSQSHYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H391</accession>
    <name>T1H391_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H391"/>
    <dbReference type="STRING" id="36166.T1H391"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010710-RA">
        <property type="protein sequence ID" value="MESCA010710-PA"/>
        <property type="gene ID" value="MESCA010710"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_761387_0_0_1"/>
    <dbReference type="OMA" id="HYENGLE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16470">
        <property type="entry name" value="RING-H2_RNF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000215">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039133">
        <property type="entry name" value="RNF25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13198:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13198">
        <property type="entry name" value="RING FINGER PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="6">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="84"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="204"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="257"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41653" checksum="D8DF4C06F81CF254" modified="2013-10-16" version="1">METTILNDNNLQITLQVIPSESYPDVSPLFKLLRPRGLDDQRLDDIKKACIAKLEESVGFPVVFDLIEVIREHMTQSNLPSGQCVVCLYGFEEGDEFTKTSCFHYLHSYCLARHLAASKKNYEEEQDKLPSWLRKTSSPYQPNCPVCREPIEDCSEKDLRQLQLKMASLFLIQKSKGAIIDVDAENSGNLISIQTEEEARLELERKKAASETNGHYENGLENGVNDDSENGEEEEEEEEEDDCANYSQNYTEYFKNFNNKRSNKPNSSEDSSNNTTDPHLQTTNGATMPFAPNVQQSQRIADPTNNNYDRNHHYNRRHHYRGGRRHQYHKHRYDRYESSGHQNDAAATGESQKGQCANTSGNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3B1</accession>
    <name>T1H3B1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3B1"/>
    <dbReference type="STRING" id="36166.T1H3B1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010731-RA">
        <property type="protein sequence ID" value="MESCA010731-PA"/>
        <property type="gene ID" value="MESCA010731"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1295667_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF27">
        <property type="entry name" value="OSIRIS 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010731-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="23448" checksum="7F6A8BA4AAE9B497" modified="2013-10-16" version="1">MDTNQRYYDYQTDGYLPEEPSTARVVASRYLLPFLLGIKFNLATIIPLLFGAVVLLLKKATFLAKLAVYVSTLFGVGGAFTLANLGGGLGGGLGFGGGGHFQHHSFGGGGGGGFGGFSPIKSSASYGAADSYDTGYGLLRNERQFKFDKPRSTNEPVSTYDKFYEYEKNETSNWTFVMVWICAEDGRKCPVKKMFLYKPEWISKERKAWNQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3B8</accession>
    <name>T1H3B8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Complex I-B14.7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase subunit B14.7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA11 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3B8"/>
    <dbReference type="STRING" id="36166.T1H3B8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010740-RA">
        <property type="protein sequence ID" value="MESCA010740-PA"/>
        <property type="gene ID" value="MESCA010740"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134185_2_0_1"/>
    <dbReference type="OMA" id="SIEQGWE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039205">
        <property type="entry name" value="NDUFA11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21382:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21382">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010740-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="12338" checksum="4733BC0452696BA5" modified="2013-10-16" version="1">MSLLKSQYYDSPEGTDAFGKIVATNKYAVLGGLAWGTIDVLMISKPKGYLPILARYAYNVGPMMGMASAFTLGTLVATNVRGKDDRLNYFIGGACAGGVYGAWRRSFHAGAVAALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3C9</accession>
    <name>T1H3C9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3C9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010753-RA">
        <property type="protein sequence ID" value="MESCA010753-PA"/>
        <property type="gene ID" value="MESCA010753"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2475300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010753-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9380" checksum="C0E15C946E7A23BE" modified="2013-10-16" version="1">SAPVVVTTATTTANPAPGVPLAQTTYYATQITPAVVSQTGTNYPVQQSMVQTTPYPVGNQTMPMPQNQGFNQQPPSYDQVQSNANMYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3W1</accession>
    <name>T1H3W1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3W1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010956-RA">
        <property type="protein sequence ID" value="MESCA010956-PA"/>
        <property type="gene ID" value="MESCA010956"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707620_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010956-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8462" checksum="5B7A6AFC68076485" modified="2013-10-16" version="1">MRRILIGSSNKQTYLAYHNQPQLYSTDNFWSSKKSRGIYFSSKIWGKPPYTINLIYGLVKFTSLKPSPTELSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4L8</accession>
    <name>T1H4L8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Lysophospholipid acyltransferase 5</fullName>
            <ecNumber evidence="16">2.3.1.23</ecNumber>
            <ecNumber evidence="17">2.3.1.n6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.23" evidence="16"/>
    <dbReference type="EC" id="2.3.1.n6" evidence="17"/>
    <dbReference type="EMBL" id="CAQQ02087261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4L8"/>
    <dbReference type="STRING" id="36166.T1H4L8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011231-RA">
        <property type="protein sequence ID" value="MESCA011231-PA"/>
        <property type="gene ID" value="MESCA011231"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536303_0_0_1"/>
    <dbReference type="OMA" id="HMAYLLM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071617">
        <property type="term" value="F:lysophospholipid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF14">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00026120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011231-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="19677" checksum="6D15A9B8E1304043" modified="2013-10-16" version="1">MINIVGFIASKANAPEPAIRLLITILAGYPIALFYKSFLEGKINKICKHLYFLVLGVLLCIFNYGSDTFHSGIAVIITYFLSILLNGSLLVQVNFVFHMAYLLMGYYFTESNDYDILWTMPHCVLVLRLIGYGFDVADGKSDETKLSKDQKENGIKETPSLIELAAYSYFPSSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Q7</accession>
    <name>T1H5Q7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5Q7"/>
    <dbReference type="STRING" id="36166.T1H5Q7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012040-RA">
        <property type="protein sequence ID" value="MESCA012040-PA"/>
        <property type="gene ID" value="MESCA012040"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083737_1_0_1"/>
    <dbReference type="OMA" id="SENENNW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012040-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16910" checksum="83B2B01D39F033F7" modified="2013-10-16" version="1">DEIDNFMSQSENENNWEKTLQKLDEQHGKYKFMEFNLEARKRKLKQQIPDLERSLQMIEILREQKEDKDTQFLLSNQIFCKTTIPPTKTVCLWLGANVMLEYPLDEAEGLLKANMESAVANLKVVEHDKDFLRDQVTTTEVNMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5W5</accession>
    <name>T1H5W5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipase B-like</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Putative phospholipase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the phospholipase B-like family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="T1H5W5"/>
    <dbReference type="STRING" id="36166.T1H5W5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012098-RA">
        <property type="protein sequence ID" value="MESCA012098-PA"/>
        <property type="gene ID" value="MESCA012098"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027106_2_0_1"/>
    <dbReference type="OMA" id="RPVRNDM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007000">
        <property type="entry name" value="PLipase_B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370:SF3">
        <property type="entry name" value="PHOSPHOLIPASE B-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370">
        <property type="entry name" value="PHOSPHOLIPASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04916">
        <property type="entry name" value="Phospholip_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012098-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="511" mass="58375" checksum="E45117950CC8B775" modified="2013-10-16" version="1">RNPKTAETMLKVVGASVNKTRIGTYILVGAGMLVIGAFFIAGMERPQYDGTYCAVANYTKNIGYKFHYLKQQTDLVNIPKGLARICYKDSIYENGWAQLELETQRNYPDDIQAYAAGFLEGAQTWFQINNQWRNTISSICKISDDSERFCEWLRGVLLTNYEKMMEHAKDNANRDPYWHQIFLYYKQLEGMKEGYDAWTRAKPDIDVEEIPLEDFLILNSVSDISDFKVYYDNYILDAESIPSDFELPGSTMFVKIFKSLESSGWFTLFGHTSAGSYSSMLKIHKRYKLRYNFNKSFSAVHGMDISFTGYPGILASTDDFYIVKGKKIHSLISGVPIKNANLTLWMSKKEKIDEAVPLSARIMAANRLADNGFKWSKYMSRYRVTGSKQWAFIDLKKIDSLPQSSENEGDSSSLKDAVWISEQLPGYWHSESVKEEILLNNFTWIGSGIPYFNKTLELSGISNNENIATVDECFSYFGLMDKFLRNISFRGDLLHGSDGQPIPYGNIDIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6T3</accession>
    <name>T1H6T3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6T3"/>
    <dbReference type="STRING" id="36166.T1H6T3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012416-RA">
        <property type="protein sequence ID" value="MESCA012416-PA"/>
        <property type="gene ID" value="MESCA012416"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023008_11_2_1"/>
    <dbReference type="OMA" id="ICKTVRI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000353">
        <property type="entry name" value="Synaptotagmin 7, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000140">
        <property type="entry name" value="synaptotagmin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF344">
        <property type="entry name" value="SYNAPTOTAGMIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="5"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012416-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="22122" checksum="CB84F62FCE652FF1" modified="2013-10-16" version="1">DNEEYVGQIHFSVEYDYQNTTLVLVLQGKELPAKDLSGTSDPYVKVTLLPDKKHRLETKIKRRTLNPRWNETFYFEGFSTQKLQNRVLIKKSKVLLIIIIQVIHLHVFDYDRFSRDDSIGEVFMPVCQVDLSRKQTFWKNLKSPASDKCGEILISLCYQPSSSILTLSLIRARNLKAKDINGKSGEWSKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6V7</accession>
    <name>T1H6V7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6V7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012439-RA">
        <property type="protein sequence ID" value="MESCA012439-PA"/>
        <property type="gene ID" value="MESCA012439"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016144_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
        <location>
            <begin position="413"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012439-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="711" mass="78080" checksum="28696D898D1B3E35" modified="2013-10-16" version="1">PTFSERSQLKYARRLVVKLGSAVITREDNHGXXXXXXXXXXXXVAECHLEGREVMMVTSGAVAFGSQKLTQELLMSLSMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNVGRVLRRTKLAENLELKQVTVPIGVLLVIFESRPDSLPQVAALAMASANGLLLKGGKEAAHSNKALMDLVKEALASVGAENAISLVSTREEISDLLSMEDHIDLIIPRGSSELVRHIQEQSVHIPVLGHAEGVCHVFIDKDADLDKAIKIVRDAKCDYPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATASKFQSSIDSACVFHNASSRFADGFRFGLGAEVGISTARIHARGPVGVEGLLTTKWILNGTDHTAADFAEGGPRTWLHEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7H0</accession>
    <name>T1H7H0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7H0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012707-RA">
        <property type="protein sequence ID" value="MESCA012707-PA"/>
        <property type="gene ID" value="MESCA012707"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275061_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012707-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24276" checksum="C77CD7F2252195CA" modified="2013-10-16" version="1">LHKVHCTHEEMQVQIVLQSQEDKSASHVYLEGLKGYPDVRCQPVIEGGLAEFKLSLKDFFECGVTRMVNKITXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGTPLSMEIYLDDLSAPVYGLGVTHMQVTDTNTQEETIIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA27</accession>
    <name>T1GA27_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393326">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000071-RA">
        <property type="protein sequence ID" value="MESCA000071-PA"/>
        <property type="gene ID" value="MESCA000071"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226197_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000071-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="12123" checksum="F615CB78F3CA9A62" modified="2013-10-16" version="1">MNGLIEAFQHLRTVTNRASIPTATSSSYSTIFNWICWHASDPYNSAGSQGYQLDRFGRTSPPFQLFVFLKETFSILRKEDRKAGMKASRQDNGRGTHPTERSKYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA70</accession>
    <name>T1GA70_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA70"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000118-RA">
        <property type="protein sequence ID" value="MESCA000118-PA"/>
        <property type="gene ID" value="MESCA000118"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3144425_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000118-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="49" mass="5512" checksum="63EEB930CA29604D" modified="2013-10-16" version="1">MISKDLHKYNYNSLDMLRGKSVIEDSDTLLAKGLIPIIDLAHCGTEEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAY2</accession>
    <name>T1GAY2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02141232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAY2"/>
    <dbReference type="STRING" id="36166.T1GAY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000399-RA">
        <property type="protein sequence ID" value="MESCA000399-PA"/>
        <property type="gene ID" value="MESCA000399"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393316_0_0_1"/>
    <dbReference type="OMA" id="PCDIREK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000399-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="23100" checksum="16BC08CA6F64CE0C" modified="2013-10-16" version="1">MFSAKRHMVHDFDDIYKKYKNKERFVGVYNWREPQVLITSFHSDWQLISPALNSNRIRAYYPIIEETSKRLVNFLNKTCKNPPKDGLEAKELAMMYTLDIVGAGIYGIDSGTFSEDHPTELFKMAKNLFNQDLFFFVYMSIVTMFPKISNFFIVSFFSDKVGKFFLNVVKETVKYKTDYTETRANFLGYLMELNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAY9</accession>
    <name>T1GAY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">CDP-diglyceride pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">CDP-diglyceride synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02037786">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAY9"/>
    <dbReference type="STRING" id="36166.T1GAY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000406-RA">
        <property type="protein sequence ID" value="MESCA000406-PA"/>
        <property type="gene ID" value="MESCA000406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023471_3_0_1"/>
    <dbReference type="OMA" id="CTIPLSF"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF8">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="25749" checksum="4C1A8D879BEB7AB5" modified="2013-10-16" version="1">MYCVGFVWFVLSLVKKYYMKQFSLFAWTHVSLLIVVTQSYLIIQNIFEGLIWFIVPVSMIVCNDVMAYMFGFFFGKTPLIQLSPKKTWEGFIGGGFATILFGFLISYFLCHYQYFVCPIEYSEELNSMTMACDPSPLFTKQEYSLALFGLKSTSIKMYPYVWHSLSLSLFSSIIGPFGGFFASGFKRAFKIKIFSLKPEQQLQIFDVLQENLKGRNLLNLNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB26</accession>
    <name>T1GB26_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000447-RA">
        <property type="protein sequence ID" value="MESCA000447-PA"/>
        <property type="gene ID" value="MESCA000447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1067825_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="261" mass="28950" checksum="8E05592DB9625526" modified="2013-10-16" version="1">MGDITGTNRSSRERDSIISSSAAIESSPVEEPEEKAGSSRKGEEVGGGEVNVMDLGNKMNLENNNICSAVTPTTATTTTSSFLTPPNKDVVMISSPTGESTPHNMISSTSIALNQKYKRFIRKSVDGNQFFGDVKSPLHYLDSSEVMQHHVQGQPQGQNQTPIMPQHIQGQSQSQPQNIQHVQGPSPHVTQPQMIHVQNQQQVMQQQPQVLQHMIQNQQVVQHQQMIQQPIQQQMVTQNQTQNYQQSSSQVQASQGYQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB61</accession>
    <name>T1GB61_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000483-RA">
        <property type="protein sequence ID" value="MESCA000483-PA"/>
        <property type="gene ID" value="MESCA000483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778780_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7740" checksum="E649E6978A5360D6" modified="2013-10-16" version="1">MESCSETARFCNFHLLVPRSTTLPTPDNSSRNKPQNPSSNMGLCIILKYSLSPIMVLDVGNLNDMKRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBK3</accession>
    <name>T1GBK3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SLC26A/SulP transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02022558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBK3"/>
    <dbReference type="STRING" id="36166.T1GBK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000637-RA">
        <property type="protein sequence ID" value="MESCA000637-PA"/>
        <property type="gene ID" value="MESCA000637"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478623_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="6">
        <location>
            <begin position="36"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000637-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20232" checksum="F48A0FA9CFD47707" modified="2013-10-16" version="1">MVDPRFCSRSYSWIDFCSTVNSLWSCSWTTSSIWSYSAFMSSFMYIIFGTCKDLTIGPTAIMALMINAHAVISPDYAVLASFLCGCVMLLLGFLNLGFLVQFISIPVVTGFTAAAAATIGSAQINTLFGIKNNRSNELVDAWINFFSNLDKISLYNALLGVLTLVFLILMRFFKDVKFLPKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC05</accession>
    <name>T1GC05_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 188</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNEP1R1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02029470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC05"/>
    <dbReference type="STRING" id="36166.T1GC05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000800-RA">
        <property type="protein sequence ID" value="MESCA000800-PA"/>
        <property type="gene ID" value="MESCA000800"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138149_0_0_1"/>
    <dbReference type="OMA" id="ITTRARC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019168">
        <property type="entry name" value="NEP1-R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996:SF1">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09771">
        <property type="entry name" value="Tmemb_18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000800-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13784" checksum="1DB28B1163E86079" modified="2013-10-16" version="1">MESSACDDLKSFERRLTEVVTCYQPAAKKWRIILAVIASATAIGAYYWLKDPDTSIVPFNESLFNHKIFSLAIISLIMLFLAGVDNLVIAPKIITSRTRTVLSNYNMSCDDTGKLILRPRPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCG6</accession>
    <name>T1GCG6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell adhesion molecule Dscam1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCG6"/>
    <dbReference type="STRING" id="36166.T1GCG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000978-RA">
        <property type="protein sequence ID" value="MESCA000978-PA"/>
        <property type="gene ID" value="MESCA000978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_689977_0_0_1"/>
    <dbReference type="OMA" id="HHYERIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021012">
        <property type="entry name" value="Dscam1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12355">
        <property type="entry name" value="Dscam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Cell adhesion molecule Dscam1 C-terminal" evidence="3">
        <location>
            <begin position="289"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="400" mass="44464" checksum="6F4F61034404E465" modified="2013-10-16" version="1">MHRTIAPLDDGYYGPGNGNTRIRMPAWMPEWLDINFMVPLIATVIVVAVGILVICVALSRRRSDDFNGGQKDVYYDVVYNQTMGPGATLDKRRDIRDELGYIAPPNRKLLQFLDQTITPAIDLNEELTMRSNHSTWDPRRPNLYEELQPPPVPRRGHGHSHGAEPCYHTHRPGMEDEICPYATFHLLGFREEMDPTKAMNFQTFPHQNGHNGGPVHAGTMGPPGHPGHNHSRSGSQSMPRANRYARKNSQGGNSSIYTPAPEYDDPANCAEEDQYRRYTRVNSQGGSLYSGGPEYDDPANCAPEEDQYGSQYGGGYGTPYDHYGSRGSVGRRSIGSARNYNGSASPEPPPPPPRNHDMSNSSFNDSKESNEISEAECDRDHGPRGNYGAVKRSPPIKDPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCP3</accession>
    <name>T1GCP3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001063-RA">
        <property type="protein sequence ID" value="MESCA001063-PA"/>
        <property type="gene ID" value="MESCA001063"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822050_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001063-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="39246" checksum="CDA45B331CBBF3DF" modified="2013-10-16" version="1">MDTLLNYYPVITTLCGKLSYRELKSLSCASKGWWEATNSLIVNKTLLKIDKPCCVKFQRKYKYVKIDKIDFEMVLPTLPDSIAIFNFKQIQIKDFQIFKRFKKLKGLWFYLCDFPAEIPNKEALIFGLESLHIMLSNTNEIALVDNFIKANNKIEDLQIQVSIYDDESSAEIMVNTVLANIHLPYLKEIYIRGWDNSDFNCGLIGIFKNISSIKSCKLKYLKLSDTIFENIHNTCKKLETLSIVKCENLTGHFLKDIKKVPTLKHLELFKSEINVEELQKIYIPQLTSFRVSLSEDSPVSGMNSIQTQSMIKHMINLTVLDLTIYKACIKRQFWTIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDI1</accession>
    <name>T1GDI1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001368-RA">
        <property type="protein sequence ID" value="MESCA001368-PA"/>
        <property type="gene ID" value="MESCA001368"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707636_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001368-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8271" checksum="EEA19F22ACB6F7A5" modified="2013-10-16" version="1">MEAGLKLKKPEKGGELDCPYQELIGGLLLAARITRPDINFAVNYFLAWSEANPSIFERNAFFQNNTYFVADEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDQ7</accession>
    <name>T1GDQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001450-RA">
        <property type="protein sequence ID" value="MESCA001450-PA"/>
        <property type="gene ID" value="MESCA001450"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_42_23_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001450-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="13324" checksum="CBD3B8BAB9D54904" modified="2013-10-16" version="1">MNRISRDFTHNYSENRGSTYRQPKSEFKPNSRYYNQSYELRYPCQVCGKSYLRKPLLKRHELYECIGIPPKFTCEYCPSRYRRNEDLKRHLYKVHNIITQKNISLKHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDU4</accession>
    <name>T1GDU4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDU4"/>
    <dbReference type="STRING" id="36166.T1GDU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001494-RA">
        <property type="protein sequence ID" value="MESCA001494-PA"/>
        <property type="gene ID" value="MESCA001494"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2564992_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001494-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="8955" checksum="44B11AA9EC3C084C" modified="2013-10-16" version="1">MAAEIKPAVSKANDRFNTSKKPELLSKLEGSADDVNAALMIPGENAVISVSDDKFCDLKSLYENIDFIDFVKDFSISGNQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE01</accession>
    <name>T1GE01_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001556-RA">
        <property type="protein sequence ID" value="MESCA001556-PA"/>
        <property type="gene ID" value="MESCA001556"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852256_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001556-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7828" checksum="54062B1582FE4817" modified="2013-10-16" version="1">MLRPLIIGPYIDMSLYHGWALSIIYGFHMERPEYNKAVTSKDKLKWRSIHIKTRSKRREVVKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEG7</accession>
    <name>T1GEG7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001729-RA">
        <property type="protein sequence ID" value="MESCA001729-PA"/>
        <property type="gene ID" value="MESCA001729"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323039_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001729-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11627" checksum="C7D22595293E9DCF" modified="2013-10-16" version="1">MKLRQIPASNAIDHHLLWWHYLRVPINIFIEILNCLAIQSNIKTILNLSGSKDNLITSVHGLRVFSLLWTILVHTYLQSFFIGENKVLRKLKNKIFYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEN7</accession>
    <name>T1GEN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEN7"/>
    <dbReference type="STRING" id="36166.T1GEN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001807-RA">
        <property type="protein sequence ID" value="MESCA001807-PA"/>
        <property type="gene ID" value="MESCA001807"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087571_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588221" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="21"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001807-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13201" checksum="92A67D4D53B8FE90" modified="2013-10-16" version="1">MHASLVFCVLALSWSGVLCRPQITSITQETIVSEGDSADFNCTVKDHGSSSLEWKKTNLDKRDDSEVLLALRDTLNFPDSRYTVKIIPSKDGTVIYHFNIKKIEASDMGQYKLLEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF24</accession>
    <name>T1GF24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001953-RA">
        <property type="protein sequence ID" value="MESCA001953-PA"/>
        <property type="gene ID" value="MESCA001953"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1483639_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001953-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="21139" checksum="160BE279563FD258" modified="2013-10-16" version="1">MASQNQFEASQTRPKQFYFNQSVNRESYSSIYLSSKRAWYNNNTCDIIENGKYEHEFGKPEYGSRSENLPRSSVRNGNTGDRRDTTISRNQIKFNRVSSDAICSLFAKRAENCPDSEGFQQLVVQPLYRKFSRGNPRSFSQFRHRARAVEDYKRELSAGRKQFGGVKHVCGRNRRHGRVSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFA0</accession>
    <name>T1GFA0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002036-RA">
        <property type="protein sequence ID" value="MESCA002036-PA"/>
        <property type="gene ID" value="MESCA002036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402163_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10837" checksum="D119D48B97C60F02" modified="2013-10-16" version="1">MVPSPQNPSQLQVPPEGTKEKKVREMLEFMEDCNYRHESNNERLQIGGDLYREMNQSSKHTIITHQLKANDAEPYLKFCKRCSVKDEESPKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFR9</accession>
    <name>T1GFR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">alkaline phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 2 Zn(2+) ions.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02039026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFR9"/>
    <dbReference type="STRING" id="36166.T1GFR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002218-RA">
        <property type="protein sequence ID" value="MESCA002218-PA"/>
        <property type="gene ID" value="MESCA002218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032411_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF91">
        <property type="entry name" value="ALKALINE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588249" description="alkaline phosphatase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13795" checksum="F8D81C8622AB82F2" modified="2013-10-16" version="1">MKVFLFIIAALAVISPLFASESDHKMHPLFSFKKRLQKRAMPLIAFNPANTEESGDYWRSVGQELLKQQLKKNTINTNKAKKVIMFLGDGMSIPTLAAGRVYMGGEEKQFSFEKFDYSGLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFX3</accession>
    <name>T1GFX3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFX3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002275-RA">
        <property type="protein sequence ID" value="MESCA002275-PA"/>
        <property type="gene ID" value="MESCA002275"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1919450_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002275-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="15022" checksum="0B90AF9398B4A8A2" modified="2013-10-16" version="1">MPLKVKTWSCALQVRTEFAAIRMTHQIHKFHSTFFIVILFLTILSLSCVLSLEINEESSIIAEETTDVIETSTGSSDLIEKDEDDEENNNDNISFWGFALDIVESVITNWYSGNNTSHQVLSNFIIFHSSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG32</accession>
    <name>T1GG32_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002338-RA">
        <property type="protein sequence ID" value="MESCA002338-PA"/>
        <property type="gene ID" value="MESCA002338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485932_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10012" checksum="2C135BD5A4DA0702" modified="2013-10-16" version="1">MFYLRVTPSVNREVNSNVVQSKLWWIMNFKGGSEIPQILNEENRGVQSISKPSTSNLLRYSDSLKGNRILDKFNRKAKRGSPLQTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGJ2</accession>
    <name>T1GGJ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Metalloendopeptidase</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02051875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGJ2"/>
    <dbReference type="STRING" id="36166.T1GGJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002513-RA">
        <property type="protein sequence ID" value="MESCA002513-PA"/>
        <property type="gene ID" value="MESCA002513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017286_2_3_1"/>
    <dbReference type="OMA" id="NSIMHFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="8">
        <location>
            <begin position="52"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="231" mass="26764" checksum="52B4D73E1EBCBDB3" modified="2013-10-16" version="1">MIRLKPLGLVGHIECPKAISHKIKNDSYLLEGDMIVSIKQAEWLFSKKLNRYVISSPFSKWPGKTVYYQFVEFFPEKVIKLVKESLNEVMRFTCLQFREGENSDGKHISIKNYENKCWSSVGYSEVSEQIIVLSETCTSKDIIHVFLHAAGFFHQQSSPERDNHVEIKWENILEGYKRNFDKYAIDTSFGQPYDFNSIMHFRRDAFSSNGQPTILAKTFPESLLMGEAKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHK5</accession>
    <name>T1GHK5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002909-RA">
        <property type="protein sequence ID" value="MESCA002909-PA"/>
        <property type="gene ID" value="MESCA002909"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073687_0_0_1"/>
    <dbReference type="OMA" id="VREQKWV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002909-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="262" mass="29583" checksum="B9B72587356A8DB1" modified="2013-10-16" version="1">MEGFEYVVVPPKQPKVCFGSGIVRECLPSTGPALSSFMRKNIGETREYPGPCDFKIKTSLIKRFANRKGYTLLASKSARMPKDLVKNKIPPLGTYDVEPQKPVKSAFQPFGSNTERDTFKDHSNTPGPPTYSKSKDTSQKICLCFGTDKFNYPSVQIVCTPINLAVCEKCLETPIGDYWYRESTDQTYCRPCMKEELSALKSCGKRNAAMNRKLADLGKFVRYRYCGFFHDHAGTSAAVQIMSKKDLIYKIRKEDYLASFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHR2</accession>
    <name>T1GHR2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155648">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHR2"/>
    <dbReference type="STRING" id="36166.T1GHR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002970-RA">
        <property type="protein sequence ID" value="MESCA002970-PA"/>
        <property type="gene ID" value="MESCA002970"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453152_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002970-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="603" mass="65160" checksum="5984329D83FFAE05" modified="2013-10-16" version="1">MCKKNLEGQSFYNKGGQLRENVPLAFIDASPSPTPRPTTETPQFETEQHIEQQRQLISSTNAKSVPIIVEDRSGPVTMTFQSLDSHLQQEDHSRIRSLTPCSQINKPAPIIPFYQAPEKLLFEECDPTKSQLFDRRSVSPYSNRAKSPAPGPPPNPLNSIRAPRLKSPVREGFGGFVANIPRPQAGSIMTGQSAQGQLISNYQASNISGSQSFVNKPEIIQQGTIGDMSYERRENESRMAEEKRAQMESKTTTQVGNAQIQRHRKAADLTKFPQHQFQTDAKFPTKSLLPSYDEPKQPAYVVPTATPNKVAGQQPGYLAQSQNPYAPSSGYSASSIQQQQKQCSSLMSSSSLNATNSLTNSSAFANTNVSAPSIKPPFGGSIGGGQPIYKPPDSQQLQQRQSSISSTNQNINISSDINSGGAGSNKGGAISARSAPKRGYGILNKAGSMQLTNKPAEAAPIQKSTADFLRTLGRAPKQTVQNEQMNEAGKVISDMLAARLAKNAPKDGPPQEMFKVSKFVPQTTYDIPNPIVAQNASSTFNIPKPSSYVTPTPGNNIPKPPAPPQSTYQASGNIPKAPTPPQSTYQSNVTIPKPPTPPQSTYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI61</accession>
    <name>T1GI61_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UFSP1/2/DUB catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041745">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041746">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI61"/>
    <dbReference type="STRING" id="36166.T1GI61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003129-RA">
        <property type="protein sequence ID" value="MESCA003129-PA"/>
        <property type="gene ID" value="MESCA003129"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1435961_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="2">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="2">
        <location>
            <begin position="111"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003129-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="21273" checksum="07DF3D45B4B9D4A7" modified="2013-10-16" version="1">MDDQGNFRKQSAAAMQRAVYDREMSVTDYYERQVGLRAAESHGVDNATSCTKTVAPRVMSLSASSPGNIKSMVCSSVDHYASSYGDKGWGCGYMNLQMMWNLSSLLQGSEQLGCKLHNTRKWIGATEIVTVLSWLRINCQLVDFHRPTSSDGKHTELFNWVLRYFEEPRVHTPPLYLQHQASSAEVKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GII7</accession>
    <name>T1GII7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GII7"/>
    <dbReference type="STRING" id="36166.T1GII7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003266-RA">
        <property type="protein sequence ID" value="MESCA003266-PA"/>
        <property type="gene ID" value="MESCA003266"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_519346_0_0_1"/>
    <dbReference type="OMA" id="LENIDMC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052283">
        <property type="entry name" value="GenomicStab_NeuMorph_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15739:SF5">
        <property type="entry name" value="LD23158P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15739">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003266-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="59106" checksum="633C133D9E89A207" modified="2013-10-16" version="1">HESSAANSTDQSKNLKNVKNNHSEKSIETQKVSNKRKNDNSKDFEIHKEKKVENTKYIPQSLAVIKGKKYVVIKRPTVHQNFKKKPQDVIKKNKGAKSIAKEIISTNSTSSSITTASATAELLCCARVCRLWSLVSNHKLLWRTVRMKNSYVGNWKGFANALQSNSTVHLDMRKMITTNSDKMWLDFSNNISCVQSLENIDMCRCPTNVIENLFENNKNLKVLNACSISANKGLCLKNLSNMENLVELRLRGIEPLTLKSNELGCTTLPNLVQLSLTTFANLTSEDLTFLKHLTKLVSLELGDCSLLSSKFPKDILPSLKTLKRLRLENGKDDCPTEEILETISTMTNLTQLELINFDLKAYLDKKLALCQNLKRLLLIPTYQQQSATTNHIILNGILKLTSLEWVVTIELIRVTALYVVQYDEGSRTSKSQKRKSSTGSTGSSNSKDGYDECIPILKPIPGVVSEEEYFDPIPLLQPNNPDVMPQIEILPLEKIKNILHTGLPATNFNIIKTPYANTWKQNLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIT6</accession>
    <name>T1GIT6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02395012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02395013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIT6"/>
    <dbReference type="STRING" id="36166.T1GIT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003371-RA">
        <property type="protein sequence ID" value="MESCA003371-PA"/>
        <property type="gene ID" value="MESCA003371"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_346928_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14089">
        <property type="entry name" value="STKc_MAPKAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000156">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="13"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="580"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="640"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="706"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="706"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="726"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003371-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="814" mass="92669" checksum="6AAF30767B31695C" modified="2013-10-16" version="1">MSYKKQLLVDDYEISSKILGLGINGKVVECISRSTGKKYALKILLDSPKSRREVSLHLRAKQCQQIVEVIDVYENSYGGEKCLLMVMEKMEGGELFQRIQEKTEGSLSFTEREAAEIMNEICSAVYFLHKRNIAHRDLKPENLLYTKRGKDAVLKLTDFGFAKETVNEGLSLQTPCYTPYYVAPEVLGSSSYDKSCDIWSLGVIMYILLCGFPPFYSNTGLAISPGMKKRIRTGQYEFPDPEWKNISQSAKDLIKGMLNVNAESRLSIEQVMMNEGFLDFLLFLKLRYLLANFYEKGKIIDNGIDEPDDVEVEEKEFITYAKIGDIYKNNRLIEFSPHTDLEDANKKSFYQIQEAFLYFKVTPNIGDDEYSNKNNKHLESAVLKIWIFQIIDDQTLNLISCQPVKTFSWQKIDITFVIKNWYSTLSFFYALYVCSNYYTKNCNLFFSYLGLTRPTIYPFPTSQYTYSMLSPEMPQVASWHTPSVYNPTASGFRNPYPSSLPINSSLSSDFSFRFSQSLLPSVHTPPHHMINTHSTMVQSSKDLANNLNTNGSAESTNQRYIREEQAKYYELARRERQLHMQMYPDWSSRTNSSRGKKRKRKQEGNDGNNMKKCRARFGLDQQNQWCKPCSPGLSTVHATGGTGLQNSSTSSSTSSNSSSNPNQLQILSSAGQAGLCLQPSPQPPTYVNLRKKKCIRYMETDCDESNLTNSHNSLQNHKSNQNPSDGELDDLSGDDEEDDIDLDQDTESLSTNSPATTAPLPLERLPVGANPRDKNNPLSVNQLTGDFLPKQLTKIMHLLWQLLQLLHHFITISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIT9</accession>
    <name>T1GIT9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003374-RA">
        <property type="protein sequence ID" value="MESCA003374-PA"/>
        <property type="gene ID" value="MESCA003374"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963437_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003374-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6434" checksum="49EAA147D9B7B061" modified="2013-10-16" version="1">MLSEQQQIHLSSPYVPPSQMLCDPHSQLCLEAQTIGTAHFPKGGVIAKLYTPVGRSDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ21</accession>
    <name>T1GJ21_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ21"/>
    <dbReference type="STRING" id="36166.T1GJ21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003460-RA">
        <property type="protein sequence ID" value="MESCA003460-PA"/>
        <property type="gene ID" value="MESCA003460"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1699903_0_0_1"/>
    <dbReference type="OMA" id="SSTHCGN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004084">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="85"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003460-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="18260" checksum="699A8F18ED64A007" modified="2013-10-16" version="1">MYKTERCLKVHNLVHLEQRPFVCQVCSKSFISNSKLKQHLNIHTGERPYKCNYCARDFTNFPNWLKHTRRRHKVDHKTGELLEKIPSYCSKQKQAKSDTNANANTKPKRKTAKRKEENPPKEKTPLEQQPVKISSPPQHSEIPKTKFELNIKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ77</accession>
    <name>T1GJ77_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Runt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02061538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ77"/>
    <dbReference type="STRING" id="36166.T1GJ77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003521-RA">
        <property type="protein sequence ID" value="MESCA003521-PA"/>
        <property type="gene ID" value="MESCA003521"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041058_1_0_1"/>
    <dbReference type="OMA" id="TPYAMPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013524">
        <property type="entry name" value="Runt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF31">
        <property type="entry name" value="SEGMENTATION PROTEIN RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51062">
        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Runt" evidence="6">
        <location>
            <begin position="56"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="298"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003521-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="430" mass="46762" checksum="C2FF46A0078B1B7A" modified="2013-10-16" version="1">MHLPAGPTMIATPPVTPQSNNGSNCQTPPSSNNNNSSSANSNMANFPPAMQEMFQSWVDILQEYHGELAQTGSPSILCSALPNHWRSNKSLPGAFKVIALDDIPDGTPVTIKAGNDENFNGELKNNTTLMKNNVAKFNDLRFVGRSGRGKSFSVTIIIGTLPTVQIASYIKAIKVTVDGPREPRSKQNFGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAVAAAAVHHPSLASKGSPQPVLPAAPVTQQAPEYHANGGSPHNPSSAFISQFPFNANASQSMFYNLNVLRMRPNNSDPTVIPPAHISPSSSRPSSSSPPSNHSMSRLNTSVDTSSIHDQSSEDSDDEQIDVVKSAFIPILRPQFSSSDERILQVDDSVLPSDARIKCELKAPSSMKPVLHESSQLKNTNETKLKTPAQERKTVWRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ85</accession>
    <name>T1GJ85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NK-2 homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02075124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ85"/>
    <dbReference type="STRING" id="36166.T1GJ85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003529-RA">
        <property type="protein sequence ID" value="MESCA003529-PA"/>
        <property type="gene ID" value="MESCA003529"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049543_0_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000101">
        <property type="entry name" value="NK2 homeobox 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF82">
        <property type="entry name" value="HOMEOBOX PROTEIN VND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="115"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="117"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003529-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="256" mass="29322" checksum="0F69A46852AD3E2E" modified="2013-10-16" version="1">LFDSKITPSQRSSGFHISDILNLDNQDLKVNAVLNNNNESTNQNDLNGSNLPLTNIPPVNLPNYPNIPSDLNAYHQLHRNWMREQHTEQHFEEIDDDEGHDSSGGIGNGSDGIPHKKRKRRVLFTKTQTFELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKTKRAQSEKGMINDYSFAALHHPHPSTIPSPRRVAVPVLVKNGKPCEDGRGQEFQLPGGLTAGHLAYQHHQLLHGPHGNKPWWQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJE6</accession>
    <name>T1GJE6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:43816"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43817"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:43818"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the taffazin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02086847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJE6"/>
    <dbReference type="STRING" id="36166.T1GJE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003592-RA">
        <property type="protein sequence ID" value="MESCA003592-PA"/>
        <property type="gene ID" value="MESCA003592"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046747_4_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577419" description="Tafazzin family protein" evidence="14">
        <location>
            <begin position="24"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="15">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003592-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="150" mass="17258" checksum="431F2DEDB8053B70" modified="2013-10-16" version="1">IKLNNNIYVFLFLLGKCIPVIRGAGVYQPAVDLCIQKLNLGDWVHIFPEGKVNMTKEFLRFKWGVGRIIYESTKIPVILPVYHLGMDDKCTMNIGKPIDLSNLILDLKDRNVPEPEARKIITDAIQVEMEKLRVETESLHYKHNNNVIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJI1</accession>
    <name>T1GJI1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJI1"/>
    <dbReference type="STRING" id="36166.T1GJI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003630-RA">
        <property type="protein sequence ID" value="MESCA003630-PA"/>
        <property type="gene ID" value="MESCA003630"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_3_1_1"/>
    <dbReference type="OMA" id="FLYHEMM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003630-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="32478" checksum="11FA83503B5A1927" modified="2013-10-16" version="1">MDAIRNGWFSELQDTLWPGQSFSLKVKKVIHEEKSNSKTSKFWKQTFGMCLVLDGIIQCTLRDEFSYQEMISFLPLCCHPNPEKVLIVGGGDGGVAREVVKHPLVKEVHQVEIDDRVVELSKQYLPSMACGFKDPKVKLTIGDGFDYMKNHKNEFDVIITDSSDPVGPAISLFQESYYELMKNALRPGGIVCSQGGTYWTDLEHIKKTIEGCKNHFAQASYALTSVPSYPCGHIGFVIGSLDGKANLKEPAVTFTKEQIDKLNLRYYSSDVHRSAFAVPRYVEKFLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJN9</accession>
    <name>T1GJN9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJN9"/>
    <dbReference type="STRING" id="36166.T1GJN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003689-RA">
        <property type="protein sequence ID" value="MESCA003689-PA"/>
        <property type="gene ID" value="MESCA003689"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270476_0_0_1"/>
    <dbReference type="OMA" id="YAGITNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577426" description="Peptidase S1 domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003689-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11161" checksum="E2C73608AE8F5F81" modified="2013-10-16" version="1">MKAFIAIILLASSVLTKVVPGIPNGRIVNGVNATVKQAPYIVSISSYKTYDHICGGSIIDKEWILTAGHCLPRSGKVQIYAGITNTTNRTNGQQRFSDYAIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK64</accession>
    <name>T1GK64_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK64"/>
    <dbReference type="STRING" id="36166.T1GK64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003881-RA">
        <property type="protein sequence ID" value="MESCA003881-PA"/>
        <property type="gene ID" value="MESCA003881"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_32_1"/>
    <dbReference type="OMA" id="HEAMTSD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003881-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9629" checksum="D93AD805CBDA0F96" modified="2013-10-16" version="1">MSHTNLTNSGQQPIVKIGHYLLGATLGIGTFGKVKIGEHILTKHKVAVKILNRQKIKSLDVVGKIRREIQNLKLFRHPHIIKLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKF0</accession>
    <name>T1GKF0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003973-RA">
        <property type="protein sequence ID" value="MESCA003973-PA"/>
        <property type="gene ID" value="MESCA003973"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576587_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003973-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9050" checksum="71B064285EE25972" modified="2013-10-16" version="1">MINLLYESQWQSTVTKPNYDISSVLLEISHMAAIIGSNVETKNMTKNHGAGTANRTAEEIRKIMLIIRSAIPMDSDPVFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-01-19" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKQ6</accession>
    <name>T1GKQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GKQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004083-RA">
        <property type="protein sequence ID" value="MESCA004083-PA"/>
        <property type="gene ID" value="MESCA004083"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1416635_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="22365" checksum="545B6CB6F432F1E3" modified="2013-10-16" version="1">MNKIRNTPKISSKDIDNLHLSLMANVNKVTVALNNKLKEQRQAEEQERLRKVQLELDMQRKAKEEEERKLREEEENRRLKAEMEARRKAEEAQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQERRDHELAMRLANESNGQVEESPPMIRNGLQNEVSSFMAPNKLIRYCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKZ7</accession>
    <name>T1GKZ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004180-RA">
        <property type="protein sequence ID" value="MESCA004180-PA"/>
        <property type="gene ID" value="MESCA004180"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707604_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004180-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8364" checksum="815D2E3FE7F71799" modified="2013-10-16" version="1">MIRDDFGFYLIAFNSSILPVSETSTLDAVLPTRDHRFSRLEKGKGNTDLGKNRKRKNTLYNNNNIAYKILATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLK5</accession>
    <name>T1GLK5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLK5"/>
    <dbReference type="STRING" id="36166.T1GLK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004412-RA">
        <property type="protein sequence ID" value="MESCA004412-PA"/>
        <property type="gene ID" value="MESCA004412"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144571_0_0_1"/>
    <dbReference type="OMA" id="ACCELDE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF190">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004412-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15231" checksum="6A3ECF423C59B73E" modified="2013-10-16" version="1">SVLLRRNNKIILYCKGADNVIYDRLSTSQTTIKTQTQEHLNKFAGEGLRTLALAERVIEENYYYNWIERLQHASVALDKREEKMNVLYEEIETDLILVGVTAIEDKLQDGVPHAIANLQMAGIKIWVLTGDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLQ7</accession>
    <name>T1GLQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02011315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLQ7"/>
    <dbReference type="STRING" id="36166.T1GLQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004470-RA">
        <property type="protein sequence ID" value="MESCA004470-PA"/>
        <property type="gene ID" value="MESCA004470"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182497_0_0_1"/>
    <dbReference type="OMA" id="STHRAYT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004470-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7532" checksum="23B014C20032309E" modified="2013-10-16" version="1">MYDDMSPIRPHTKTRSASICATSGSMEAAIYNSVAGTATPENGTPGYRRRRPATRSQSARITGARAIVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMJ8</accession>
    <name>T1GMJ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082683">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004773-RA">
        <property type="protein sequence ID" value="MESCA004773-PA"/>
        <property type="gene ID" value="MESCA004773"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870276_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004773-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="16148" checksum="7CBF02A421251F1F" modified="2013-10-16" version="1">MANRFLNFLSNTSLSHFNMSSSLLFPDEDGNSVPEKNSTLMGHFEKLPNFEFKNSKNLSKVIKFIKRKQHQITIHNTKIFNKALDCIFMNYILMCSSKIQEFEPDIKPEEEQDFFKYALSFLNHVFTFIPREEILRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMR8</accession>
    <name>T1GMR8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02102409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02102410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMR8"/>
    <dbReference type="STRING" id="36166.T1GMR8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004848-RA">
        <property type="protein sequence ID" value="MESCA004848-PA"/>
        <property type="gene ID" value="MESCA004848"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_431034_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF1">
        <property type="entry name" value="PEZ, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="6">
        <location>
            <begin position="86"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004848-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="635" mass="73707" checksum="6883211D2B722E14" modified="2013-10-16" version="1">MKRILSTFQCNELSNKLKEDLSMEYSQIPIQKTNAEYKIANLDENKILNNNQLYLPYDDNRVRVTPTMENKCGYINTSYITATIGNIQKFYIIGQTPHNKLQAIAFWQAVYESDVYLIINVGDENTYSPKEFNTIDFGQGTTDKMRWVDLDRPLSRQLKKYGSNQRIFLRVMYYVRSGVNLLTDEQTKYYYFLQLKSDLTDGKLFCDVKEVLILGLLTRQSEYDREQNKNLTYYLKTFVNSMAENFKIDADALSDAFIESIIEKNKSILYLTQSQAEVLYIQRCQQLEGYGQERFKAKKSSGQDVTLCITLSGILIITSYYANQTSEYISWHNVQNVVNIKRIFKIEQCDKTLSFVFAESELSRYFWQLCVLQHKFFMKFEKDHHDESNNNENLLEYIPRWTNKKQPQPIDMQCTPKIVTSNISLSTVNTNNNLSYNGETIKLKSLLPGYRPAPDYETAVRQMQNSMELDNINYNCNANRNNNKMMNPLLRTYPDITKDLNSTLNIESFDRSRHLYYTAHNYNFSSTPDLHLYKNNGAQYVSGSSPDLLCFNNVGKLPEKTRESINKSEALLRNKIEPIYENIPANTVFNDSANESSTSDAKLKNVSTSSNKSKKKIWKILSRKSNTVPRGFGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN71</accession>
    <name>T1GN71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005014-RA">
        <property type="protein sequence ID" value="MESCA005014-PA"/>
        <property type="gene ID" value="MESCA005014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148723_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="13088" checksum="4B86ACEB5629C39F" modified="2013-10-16" version="1">MSDITADIGTGANFQYARETYMFFVFFVLTYGNTQILVSQQTCNSLCFVLHVQWEEQEECNQLTTEITCTLYIDFGYWEPLQDIFGSLKNLPRMRQKETGLKIYIKQSIKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN95</accession>
    <name>T1GN95_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTF2-related export protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157236">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157237">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN95"/>
    <dbReference type="STRING" id="36166.T1GN95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005039-RA">
        <property type="protein sequence ID" value="MESCA005039-PA"/>
        <property type="gene ID" value="MESCA005039"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818011_0_0_1"/>
    <dbReference type="OMA" id="FKHIMNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="2">
        <location>
            <begin position="17"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005039-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16151" checksum="02686DD01E16C95F" modified="2013-10-16" version="1">MNSDPAVKKLFEECNRTAQKFTSLYYQAFDKRRHDLGAMYLDNGLLVWNGNGASGRDQIQSAVLQLPTTNHRLNTLDAQPIIDETTVGQATFCITCGGTVKYNGHEDFLHNCVGEIRRSIALKVVKTLFQHRWEPYISYIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN98</accession>
    <name>T1GN98_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02109403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN98"/>
    <dbReference type="STRING" id="36166.T1GN98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005042-RA">
        <property type="protein sequence ID" value="MESCA005042-PA"/>
        <property type="gene ID" value="MESCA005042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998253_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14706" checksum="349345DE43A02959" modified="2013-10-16" version="1">RLSTDLADCKSDVINLKRKLKDSEKEVERLKQQLKQYVQEVKKAEDLLMEKICQFLEQFYYIFMLQSVFLFVGRPISLRLTLQYNLHRKKLQSTAFLKPNRFLILSTISSVFSNLGQKASLSRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNB1</accession>
    <name>T1GNB1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393446">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393448">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005055-RA">
        <property type="protein sequence ID" value="MESCA005055-PA"/>
        <property type="gene ID" value="MESCA005055"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471627_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005055-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10427" checksum="75B06A9F8DF73399" modified="2013-10-16" version="1">MDDQVQPRTVTNIKSSRYKYVYLTPFHFRILIENVLQPFPKNQYFSSQGAELVAIGENIFYTGCNTRDIDSFHKFRKLEVVVLYIRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP35</accession>
    <name>T1GP35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02054449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005351-RA">
        <property type="protein sequence ID" value="MESCA005351-PA"/>
        <property type="gene ID" value="MESCA005351"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707607_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005351-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8008" checksum="25E303389BDD0AC0" modified="2013-10-16" version="1">MTLSLKIFELASASSVLVEVMQLLPEYESPFLLCDIILTIASLAVRRELCVAVENANGLEYVFDEKTSGPSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPC2</accession>
    <name>T1GPC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">carboxylesterase</fullName>
            <ecNumber evidence="8">3.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CAQQ02123924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPC2"/>
    <dbReference type="STRING" id="36166.T1GPC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005447-RA">
        <property type="protein sequence ID" value="MESCA005447-PA"/>
        <property type="gene ID" value="MESCA005447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161097_0_0_1"/>
    <dbReference type="OMA" id="HVANRTE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588414" description="carboxylesterase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="10">
        <location>
            <begin position="28"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14075" checksum="57BD1691BDA2BDEE" modified="2013-10-16" version="1">MPRMKSILCFLCLFVVDFALGSVSETLKVQLNHGGVLVGRHLETTKGRHIRSFLGVPYAKPPVDDLRFKSPVPFPAWEGVRVATKDGSICPQIDRMQGAILTGNEDCLFLNVYTPPIDVIKKSGPLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPM9</accession>
    <name>T1GPM9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005565-RA">
        <property type="protein sequence ID" value="MESCA005565-PA"/>
        <property type="gene ID" value="MESCA005565"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_825329_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005565-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="337" mass="39109" checksum="DDD8DE0DE52E712F" modified="2013-10-16" version="1">MNYKTIIFVLCIYFVYEANSLKILHNLDKHGIVKRDGVVFSKLTTEKYFESSYKDLKSLKVIDILNLEKIFSYFSKRRNNISDLNANLNNIYQLCDVNRENYINEFSFNSLSFEKADLIAATVNSSTILTELMDFKRGNFSPEIESHLRNNLHVKGLEFDIYKHKLKFKTTMNIAGSLRVAVDVSYDVPTLKIVDLYKITYIPEKYYYRNTIETLTKQKYKYLIDEDRYGNRCDFKPLGYDEFTSIGGGKFFFLFKDESNYKYKCNVNEETGTIQTGILQMEKNCVLTTAHSIVNGTNGLYQVIKTDNDELISQDFWNNFPWEMVGIIGGSILGAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPT9</accession>
    <name>T1GPT9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPT9"/>
    <dbReference type="STRING" id="36166.T1GPT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005633-RA">
        <property type="protein sequence ID" value="MESCA005633-PA"/>
        <property type="gene ID" value="MESCA005633"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130088_1_0_1"/>
    <dbReference type="OMA" id="LWFKEND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF28">
        <property type="entry name" value="SIDESTEP IV, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="13766" checksum="6A72DDC7CD99D351" modified="2013-10-16" version="1">MVPIISVEGVLKRQASLPCDITPLEHEDVVAMVLWFKENDGEPLYSFDARGRQFNHAKLWSSPTVFGARAFYTTAIHPSLLKIDDVTLEDEGVYRCRVDFKNSPTRNVKVNFTVIGKLFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPZ9</accession>
    <name>T1GPZ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02035455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035457">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPZ9"/>
    <dbReference type="STRING" id="36166.T1GPZ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005695-RA">
        <property type="protein sequence ID" value="MESCA005695-PA"/>
        <property type="gene ID" value="MESCA005695"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1086996_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNase Z endonuclease" evidence="11">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005695-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="256" mass="28469" checksum="66B8262C77F6DE8B" modified="2013-10-16" version="1">MNVIPYNITVILKDGKKLIRTSFLCKGRRSLEHDCPCNHRGYTSSLVAIVISYLFNCGEGTQRLAHEHRTKLARLEHIFLTRTSWSQVGGLPGLTLTVQDAGVPNLTLHGAPGLDEIFQGMRRFVILKDLKVETIDNSNGEIYEDNVMKVRAVPIRKAASQKIEAIEELDIETDETDYYSHENGNHKCPGDSKMNIVKKPRREENISEFFLAAAVSFLQTLVGPVKTFGLPFKSVTALLSLASLLKSLESSGSPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQA6</accession>
    <name>T1GQA6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit SSRP1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SSRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQA6"/>
    <dbReference type="STRING" id="36166.T1GQA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005810-RA">
        <property type="protein sequence ID" value="MESCA005810-PA"/>
        <property type="gene ID" value="MESCA005810"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017374_3_1_1"/>
    <dbReference type="OMA" id="HIPITET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000001">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SSRP1/POB3 N-terminal PH" evidence="12">
        <location>
            <begin position="59"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="SSRP1 dimerization" evidence="11">
        <location>
            <begin position="124"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SSRP1-like first PH" evidence="13">
        <location>
            <begin position="216"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005810-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="346" mass="38912" checksum="564985CF454C3E68" modified="2013-10-16" version="1">MKEKYTETALHTSIALVKKAINHHQITLTVILDIEGAFNKVSTDAIQEALEHVGTSSTVSQWINNKTGKVEQINASDIELVNFQRFVGTWGIRIFTTNGTLHRFSGFKDSETEKDKVAKLCLKGWNWGTAKFNGSVLSLDVDSKTTFEIPLNNVSQCNTGKNEVTLEFHHNDDAAVNLMEMRFHIPITETTEDDPVESFYNNVIDKASVLSASGEEIAIFKEVYCLTPRGRYDIKIFQTFFQLHGKTFDYKIPMSTVLRLFLLPHKDGRQMFFVLSLDPPIKQGQTRYHFLELSGPVYEVMGKIMKVLINRKITGPGNFIGHSGAPAVSCSFKAAAGYIYPLERGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQV8</accession>
    <name>T1GQV8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02057027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQV8"/>
    <dbReference type="STRING" id="36166.T1GQV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006023-RA">
        <property type="protein sequence ID" value="MESCA006023-PA"/>
        <property type="gene ID" value="MESCA006023"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014923_3_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018300">
        <property type="entry name" value="DNA_pol_alph_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="6">
        <location>
            <begin position="189"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006023-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="438" mass="49406" checksum="E29BF383FEDB0F08" modified="2013-10-16" version="1">MLGSQTPRTQTEGSGKIVYTFGNPSLIKSASWSSSKPQNIIPKQLIQHEGQTLTSKTKYMYDNLSERIAIDADKLFEIGQKICKKKFGEDTSLFKLVNVDEVSQDEVLTIGRIFCEFGGKLDATSTMLLGSNEFVCRTQLEAFQCISRTDRYDFWIKSSWRHFNGERNYHRQISFKSCGHPEIEQDLSIVVAAGPFTNSEDLHYDPLNDLLLYCKDNKPDCLILIGPFMDVDHAVIRGEIVLDKSFETFFEEMLASVVEVVGSDVTICCVSSYKDVCADSVYPTLPMSLKKQYPNVHMLPDPSIIELKSGLVLGITSVDVTDHILEYELAVNAGDKVKRVVNHLFNQETFYPLQPPADEEMCFDGDLASKFTKIDQIPNILILPSNQNRYIRDINNCLTINPGRLTDAKGGTFARMMINPPKEGDKLMNYVACQVRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRH2</accession>
    <name>T1GRH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRH2"/>
    <dbReference type="STRING" id="36166.T1GRH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006256-RA">
        <property type="protein sequence ID" value="MESCA006256-PA"/>
        <property type="gene ID" value="MESCA006256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="OMA" id="FAYQYET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF217">
        <property type="entry name" value="CUTICULAR PROTEIN 49AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588457" evidence="3">
        <location>
            <begin position="17"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="234" mass="25462" checksum="CC7EDAF3A1740F00" modified="2013-10-16" version="1">MLKFILVASLVCLVFGRPEGDLKKEVDQIIKQDSEIKEDGTFAYQYETSNGISAQAQGYLKTVTIPEYEGLDGKVVPQHQEQVLVQSGSYVYTNSDGQPINVTYVADENGFQPVGDSIPKAFTLILATALSAALARPQDPIPIISQESNIEPDGSYRYSYETGNGIKGEETGTLKKATNPDNNDVIIADGSVSYTDPDGNLISLTYTADDERGFVPVGDHLPTPPPILQQYRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRM3</accession>
    <name>T1GRM3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02031205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006309-RA">
        <property type="protein sequence ID" value="MESCA006309-PA"/>
        <property type="gene ID" value="MESCA006309"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_631029_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="291"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006309-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="435" mass="48014" checksum="40CCA16214DED27D" modified="2013-10-16" version="1">MTGLWFAEYMNGAGAGFISGKDRGEFPFPNKEYKTYKSNFFGFPNTPNEQVGHFGGLMREAQQRVGCALYDCGKVKSRPHSYLLACNYDAGHDAGRVYETSDIPGSKCKRRSKRYCGLCLTDFEPEDDTGPCLKVDYDLPTMWLKGKDPHNCGPDSHTGSTRKPEVITEKPPNENESDDDIHPSHEDNTGGGGGRGDADGESDVIGGGDTNINIGESDAEDMAREGGDDLAPGDFIPANTFVPPDEFCENATKVCGPNKHLFCGGSLEHTGVLGKVPNKIELNNAMKQVILRKHNELRNIIACGEPLTWDNELEWMADLNAKTCAFDHDCALSENFFTPGQIIGRASSVLPQKTRKFLTEILNFHFAEYLNTPLDVIDKYSEKVIQNPIDDVHAKIIKRSKLVKETSKIVKNKNLATIINDKIEKVGCAFYECGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSL2</accession>
    <name>T1GSL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase/retrotransposon-derived protein RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006672-RA">
        <property type="protein sequence ID" value="MESCA006672-PA"/>
        <property type="gene ID" value="MESCA006672"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608775_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="1">
        <location>
            <begin position="31"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006672-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8619" checksum="DCF4C836C829097C" modified="2013-10-16" version="1">MNSAGYTLISIRTAECLLKITTTDFVSKISKALKLLIKRLTIVLATDASEYGMGSALLHKFDDGSLHPIMHFSVTFNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSP2</accession>
    <name>T1GSP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02167413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSP2"/>
    <dbReference type="STRING" id="36166.T1GSP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006702-RA">
        <property type="protein sequence ID" value="MESCA006702-PA"/>
        <property type="gene ID" value="MESCA006702"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181182_0_0_1"/>
    <dbReference type="OMA" id="VTHMTNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000093">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="4"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006702-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="10964" checksum="8B5FDC76AE2FE0E4" modified="2013-10-16" version="1">PGAPPRNITAEATSPTTIAISWSPPPVDRSNGKIIYYKVFFVESARLDNEASVSTLNATNIVLDELKRWTEYKIWVLAGTSVGDGPKSHPLLVRTHEDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT67</accession>
    <name>T1GT67_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000015102"/>
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    </evidence>
    <sequence length="2156" mass="239951" checksum="9479509CF492984B" modified="2013-10-16" version="1">MTTSSVDDSRDVKETTIANADEVNSNYTAKDLDINTSKAVQEDSKEKQENNIGVIKNLGEDISAESSEDILDKFNTITDNNEPLEMPSSSVENNVDKEASRDVGKVNDENIEEPEIIKEVHGSSEGNDLDIDDSKDVQENMEERRISKEEQRDINSITGNAEPLEIPSSSQAEIIGNSGNIPFKGQDKETNASTDEVGSNLDIDTSKTVQEDSREKQEENVVIKNISKEEAGEPLEMTSTEAEVNNDKEGSEDVVKYKDINIEKAEDFDNTIPIPQRVHSNSAEDDLDIDTPKHVQENTTEEEQENIDVTKNLVSEISKEELGEQRDTNLGKNNEIASSTPPESSILNDSGILPDFQENINEDNGLQEIIHEYKITHPNSGHHEHHHHHHHLHKDPSIDLPLHKDPSVDLQSKEEPETEISSSESEDDEEVEGLEQLINENHSFIFDKNTLLKLEGESVDTNLESADKIKSQESSEDISKVDKDFVEKAGEELSKADRPEIKAEAYLTDRSDTMRSSMEEVSKEIGASEGSLSEYDMDSSKPIRVDTFDNEVSRPSNEVSDAGAKNDEKAHIDLINNEKMHSVESRKNEENVPETHKENSQNDEFDIDNSKLIRFDTIRNEVSHVSSEDSNEFVNNDEKLQTETLDSDTINENEKIHITESKNEQHLPESHKDELIDSNSDSISTDSIEASVNKNGANLENEITRPNSDGDKSSSSVEELLRNDSEELGSVVLVNKNHQDNRDGLVELLGKLLTETKDPLGVSMKEPYDAEKEKIDSRKDSVEELERKNSDEEGSITLTNAPKNFITNEYKFDRSKLEKQEDEVKQESFVVLDSSNISSKSVAEDSTSDHIKTNTSKDLTEEEIAKNTPGINESKPGEPQISKAEYDFMKHRIRKGMSDNVAVIIPTLRESTVPIKKKAHSAEGQTTRSRSPTKAVVKKPVEKKPAPKKKKLVEQDSEDNDVVIVSGRSVVSKKPLREKKWSKLENEPIKKNPPRIKRSIDYVKQNAENVASSSRARSKSKDISNISEKILAADAVLNASKAIRGSASRKSKDSVSQRVSEEEVGGDSLKGSEKEGSSSKDKQFQGIEAAISSSGNQVLIKNNKDSEKEGLPCIPSPDVLPKTNITDPNLNNNEASKLSSKNQELIEASNKNGADESRISPREQFKLKGKHVSFDESPATDIMKTLHSSVNEPELPQIESRINIEEDTKPIQEENISKIVANVKTDLPHISDNIEIDEKAEHIEKIDATKFVQDARPEVPQISNNVEIDEITKYTDIEESVSKSVEDALSIGLNIIKGVDPLNKTFSKEAVLEDRLQEEIANSKKESQNVSKYISDRLSATKKFLDESLAQLSKETHKERLSKEKVNKAEKDRSINLVNMVPDVSENFSGENREINEELEKDLQKGVMDSSAKLDSGNEDQKIRNIDDKDISIKEVSNEIESMLSKPLEDTKEELSSPSMLVKDELKELVQQGSKSKSDIDKSLISVEKEIDKAVNHGNEKATEIISDQANALDKEAKQLKENIVEAGSSIQKSLDQNIDQTLEVTYNEVNNTLSGMDKNIDKILKDESQEFQNSIKDESEGVKEIAGKGGSIIKEAVQDVGQKIKEVEKEVDKVVDHGTESVNEVSEAADIIGKSLSEDLQSVEKLIKDDSKKLKDNLENEASGVEEKVKKAELSVQDKSEEVKDSLEKEGSTIKESVKDVGHKIKDIGTSVKNIFKHKSEAAEKEVDNVVDHGTESINEVSERADIIGQSLSEDLQSVEKLIRDDSKKLEDTLQNEASDQYIDQILEDESESAEKEGNIVKRAVKDVGQKVKDVGTSVKNIFKHKTEATAKEVDEVVNQGTESVNKVSEGAESIKMSLSEDLQSVEKSLKDDSKKLKESFDQSIDQMLEDESEEVKKSLEKEGSFIKETVKDVGQKIKDVEAAEKDVNDVVNQGTECVNEMLEEASEKSGKVKKALPEELESIEKSIKDDSKKLRDTLQNEANDIEKKVKEVKEVGSSIQESLNENIDATLKDESEEVKEGLKKEGSILKEAAKDVNQKIKDVGQSVKNIFKRKSEATENEVDTEVDSGTDTVKKVSEGLDQIVQNEASEVEEKIMKVKEIESSVQKSLDQNIDKTLKDESQEGLKKEGSIIKEAKSEATENEVDKEVDSGPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT79</accession>
    <name>T1GT79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sepiapterin reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02200220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT79"/>
    <dbReference type="STRING" id="36166.T1GT79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006902-RA">
        <property type="protein sequence ID" value="MESCA006902-PA"/>
        <property type="gene ID" value="MESCA006902"/>
    </dbReference>
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    <dbReference type="OMA" id="CEKAPFW"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004757">
        <property type="term" value="F:sepiapterin reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051721">
        <property type="entry name" value="Biopterin_syn/organic_redct"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085:SF2">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006902-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26069" checksum="520B9294EF4BBD95" modified="2013-10-16" version="1">MTTDLNKKTFFLISGASRGIGKTMAIECSKKFKSGSVLVLIARSLTGLEATKSEILSSTQGLTVLIYSVDLSTANSSDYEQLLAKSLKDSSTTAGDFEQAFVIHNAGTIGDVSIKSRNLNDVNQWDRYLRTNVSSAANVPFASFTEYCTGKAARQMYFKVLAAEEKETLVLNYSPGPVETDMTVDVQKNSHDSGVRGMFENLRSEKTILTTEQTTAKFLQVLEKNQFQSGDQVDFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT86</accession>
    <name>T1GT86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histone acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02005805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT86"/>
    <dbReference type="STRING" id="36166.T1GT86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006909-RA">
        <property type="protein sequence ID" value="MESCA006909-PA"/>
        <property type="gene ID" value="MESCA006909"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015901_0_0_1"/>
    <dbReference type="OMA" id="YFQTKMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009464">
        <property type="entry name" value="PCAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06466">
        <property type="entry name" value="PCAF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="390"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="627"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006909-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="719" mass="82579" checksum="4776139910517334" modified="2013-10-16" version="1">MSADPGNSSGGNAPNHPPLQAKQHSLQKIFQRKQKVFNLPLEQKLAKISTFSACQTPECRCSGWKIPQENRHRDIESDYCPDFGDECRTAACKHPLSSHISHLSDITNEQMNEILGAIIDTENLFISMTKVEDEDTKKVYAFLFRLLRQCVASRQQPVVRGPVGDPPFETPSIAKAITNFVYSTGTQAMTEVATTFIHFVNHWNFETPSQRKTDATAEDGSSYKINYTRWIIFCQVPSFCNSLPHYETANVFGREFLRSVFKVIAQQINHKCTVEKDRFPPEKRAMLTQVPKFLDALKMELLKSDSPIWNESFKPPPSFLAQQRKRQQELVQKRGPSTSAMASNEPAFKRLKKEDDQVDLTDEAVLEAMKAISENKANDKSVILQTISDNVSRDANAKQEEYMKAIEFHVLGSSLTSPVSKQVMLWLIGIRSVFGQQLPEMPKEYINNRPIGGICFRPFESQGFIEIVFCALTVCEQMKGYGTHLMNHLKDYSIQKGVHHFLTYADENAIGYFKKQGFSKDIKLNRPIYAGFIKEYDGATLMHCELHPSIVYTQFISVVRKQRQILDELINQRHKEVQKIRPGLNCFKEGVRCIPVESIPARNSRPEESADPDKLAHQFVQVLQSVKQHTSAWPFQKPVSVADVPDYYDHVKYPMDLKTMTERLKRGYYVTRRLFMADMARIFSNCRFYNGPDTEYYRCANNLERYFQTKMKEIGLWDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTT4</accession>
    <name>T1GTT4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004834">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTT4"/>
    <dbReference type="STRING" id="36166.T1GTT4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007126-RA">
        <property type="protein sequence ID" value="MESCA007126-PA"/>
        <property type="gene ID" value="MESCA007126"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1919455_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007126-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="15128" checksum="7349726FAB80DE33" modified="2013-10-16" version="1">MEAGYLLVTVNEIIFYTSPAKVSLVAQLHLKTEPCYNANCVQIKETKYLWGDLFTYSQVWKKVLVPAPCTFDKGASEAVYSIFPWGIVYHHISPVIFMKARKNQAEREGMRRAHVKDGAAMCEAMFNFEQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU76</accession>
    <name>T1GU76_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007284-RA">
        <property type="protein sequence ID" value="MESCA007284-PA"/>
        <property type="gene ID" value="MESCA007284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264372_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="25980" checksum="E074D1D6C6D8975D" modified="2013-10-16" version="1">MTKTPLKCGLIEFNEYEQFVLFCSICQKSFEVPEFFFSHVLETHIYQKSPKPQEKVYKTYSRSKYKDLFIGNIVFEESNLKDSDDFLNFEEEKHYYLYQYNSKDPFYKNYEICNIKEIIEPKMGKHNLLGFGSIEKWESYIHSIEKVPENISQNLRIESTEHRNSSEENKDLEDFEFDIDLSGIPNPIQILEIKDEYLPSLNDDLLFKDASNKIPDIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUT2</accession>
    <name>T1GUT2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02064111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUT2"/>
    <dbReference type="STRING" id="36166.T1GUT2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007502-RA">
        <property type="protein sequence ID" value="MESCA007502-PA"/>
        <property type="gene ID" value="MESCA007502"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077496_0_0_1"/>
    <dbReference type="OMA" id="YEKTPER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF12">
        <property type="entry name" value="PANCREAS_DUODENUM HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="138"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007502-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="24189" checksum="5309FC9D55B20E2A" modified="2013-10-16" version="1">MSRSFLMDSLLSDIPVKKEESVDLRTTHLPMLPYPASYVGSYLFSLGIQQQQLQQLHQQQQAFLSPHAFLAQKRPSFDNLSSSSCSPPHESCNSPTNSTNGYDDVFGPPSSKRFRESPSKSPSAAIPVYASVPADYADSRIRTAFSSDQLLTLEREFHSNQYLTRLRRIEIANRLKLSEKQVKIWFQNRRVKEKKGGSDSPTPMDGRSGSPVEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV11</accession>
    <name>T1GV11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02100875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV11"/>
    <dbReference type="STRING" id="36166.T1GV11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007587-RA">
        <property type="protein sequence ID" value="MESCA007587-PA"/>
        <property type="gene ID" value="MESCA007587"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682314_0_0_1"/>
    <dbReference type="OMA" id="HIGDRIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007587-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="18074" checksum="596870B0A3D68932" modified="2013-10-16" version="1">MSNRHIKRKLNELQYHHIGDRIHNMEIEQRRLANANFNLSRQIANLDKLHSSMMELLENVEGIQSQIDKTVPELRGEIAKLEFTSAQLNTEQSLIREEGHNVAKSVQAVAVSVSTLQNERDIIKEIQNSIGNLQRDVEQLKSLTEIQKSLTQKQIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV63</accession>
    <name>T1GV63_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV63"/>
    <dbReference type="STRING" id="36166.T1GV63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007647-RA">
        <property type="protein sequence ID" value="MESCA007647-PA"/>
        <property type="gene ID" value="MESCA007647"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898635_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007647-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15161" checksum="9BF649D17DE6C6CB" modified="2013-10-16" version="1">MMKNGEEKSFPNGIIPFDGQVAGHIYEPGTKIIGILQDKKDDSVIYKSFGKPACGLRETKFYMSLENDCQQKNLLLQRQYFLNGADDSENTENSDPEKCEILNELKSFIPKFYGHVKFNFNGSEGNDEVIIIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVZ2</accession>
    <name>T1GVZ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007960-RA">
        <property type="protein sequence ID" value="MESCA007960-PA"/>
        <property type="gene ID" value="MESCA007960"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827297_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007960-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="15341" checksum="9821DB4909A031FA" modified="2013-10-16" version="1">MQDSTDFSPAERNFDRNLRIEEIKYICELAKRELEISSQRQNNYYNFFQVEVVYKKNYYLFNAANVFAIKIAPNYSGPYVVTNFISPTVVELIGVATPKKFIPALVKDLKEMSRAPYNHPLLLMELTTFHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX04</accession>
    <name>T1GX04_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GX04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008342-RA">
        <property type="protein sequence ID" value="MESCA008342-PA"/>
        <property type="gene ID" value="MESCA008342"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_817081_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008342-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="340" mass="38612" checksum="D47509DF32CB598C" modified="2013-10-16" version="1">MQLHVVANRXMQLHVVANRGQAAQFAQQQQQHQHPRILATPRAFNYPADPAAQQQLQQQQQQQQQQLQSIAFPERGVPEGAAAVSLTDGKNITSPTSSQLQLNQPQPATNQQPGSQTQQPLPQPQPIALQQQGIVQQQQQQQQPAQHTERTWAYYQDGTGAEHQHHHQTTIHPGYYQTLPAAAFRHAATTAAAYHGHEESLMYQNCQSVTMEQHYSHQYARSPSRRPESPPPLRNYHQTMVLIPHNAETYLQYAAVATDPKLATAHHQIHRQNILEYQQTPMKKSKNSFMAYSTIPDYDMKLILDDINSKVCREDICKGTLDMQIKLTTYYVTSDMEHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX32</accession>
    <name>T1GX32_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retroviral polymerase SH3-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008370-RA">
        <property type="protein sequence ID" value="MESCA008370-PA"/>
        <property type="gene ID" value="MESCA008370"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429563_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retroviral polymerase SH3-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008370-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10675" checksum="68F95FC759C16D7A" modified="2013-10-16" version="1">MVQVPDEKRKKLDNKAWTGIIIGCSEDSKGYRIFDPCTKKICISRNEGVFYYKSEDFLKHISINEDQNVVYFDSRSVVDLAILFYLMKELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXI8</accession>
    <name>T1GXI8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02141865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXI8"/>
    <dbReference type="STRING" id="36166.T1GXI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008540-RA">
        <property type="protein sequence ID" value="MESCA008540-PA"/>
        <property type="gene ID" value="MESCA008540"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146938_0_0_1"/>
    <dbReference type="OMA" id="MEMIMKF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11576" checksum="BA638A99E1FB1ED5" modified="2013-10-16" version="1">MQALSEEMRLGEPDADIKFTTIYPYMVDTGLCKKPKMRFADAMKLVKPHEAAAAIVKAQRLGVIEESIPKHFVYMEMIMKFLPAKAIYAIADFMDSGVESDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ7</accession>
    <name>T1GXJ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXJ7"/>
    <dbReference type="STRING" id="36166.T1GXJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008549-RA">
        <property type="protein sequence ID" value="MESCA008549-PA"/>
        <property type="gene ID" value="MESCA008549"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3299931_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008549-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="40" mass="4182" checksum="DD87EEDE54779495" modified="2013-10-16" version="1">MTMAAEIKNRVGPPSSSTDTKTNVKIDSGRAVNEPKSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXN0</accession>
    <name>T1GXN0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXN0"/>
    <dbReference type="STRING" id="36166.T1GXN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008583-RA">
        <property type="protein sequence ID" value="MESCA008583-PA"/>
        <property type="gene ID" value="MESCA008583"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608747_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="3">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008583-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9339" checksum="943623E77440F4E0" modified="2013-10-16" version="1">MIEERLFYHSGEESTSEFKQLNDIGLVEAIIKFVGPFVGEDKCQSLHTQKTKQFFYTPENDFWIVMVSLYFIIFFTLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXV4</accession>
    <name>T1GXV4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXV4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008664-RA">
        <property type="protein sequence ID" value="MESCA008664-PA"/>
        <property type="gene ID" value="MESCA008664"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2765146_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008664-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8170" checksum="B6CAA73A450BF8FC" modified="2013-10-16" version="1">MSLTNMFLLLRQKFGSYNVVHNYPPSYLQKIVGNEKIIFICKQDKSFRSSNEYIPKTTPVKREIDLDLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXW3</accession>
    <name>T1GXW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008675-RA">
        <property type="protein sequence ID" value="MESCA008675-PA"/>
        <property type="gene ID" value="MESCA008675"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530053_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008675-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9868" checksum="A3880814201A6A0A" modified="2013-10-16" version="1">MLEQLASKSGEQQEKIVKYERQLLIKRNLSGGSSSLEINRDQPKQTWKLVAYGTNFEERIPVRFKVDEKAIAIIENLQRHEIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXW6</accession>
    <name>T1GXW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02035196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008678-RA">
        <property type="protein sequence ID" value="MESCA008678-PA"/>
        <property type="gene ID" value="MESCA008678"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3034725_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008678-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="55" mass="6420" checksum="F041E50A7AEA09AF" modified="2013-10-16" version="1">MVTIKMDSTINSNHQHHHTFMQHLPMCIKKISISTIIQYHLVIITITISMTIFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY36</accession>
    <name>T1GY36_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY36"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008756-RA">
        <property type="protein sequence ID" value="MESCA008756-PA"/>
        <property type="gene ID" value="MESCA008756"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852238_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008756-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7507" checksum="F8CC23AA4E2522C2" modified="2013-10-16" version="1">MYLAVACFPSPIRYFKRTSTRDELFRGMHQFDIPSKLMKLSQMTLGNAWSCVEAASRISNNFKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZE7</accession>
    <name>T1GZE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein Diedel-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120373">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009246-RA">
        <property type="protein sequence ID" value="MESCA009246-PA"/>
        <property type="gene ID" value="MESCA009246"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152773_0_0_1"/>
    <dbReference type="OMA" id="GCKITIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025061">
        <property type="entry name" value="Diedel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13164">
        <property type="entry name" value="Diedel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577848" description="Protein Diedel-like" evidence="1">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12270" checksum="230ADCC56683C4C4" modified="2013-10-16" version="1">MKVVKKVLLFAILLGFLFQVKADCCRRTRVSFKLNDPINDSCRNYDADLAAMPPHFVDTEILQQHRRCEIQVCGDGEKPGEGIYCGIGACNLFGCNCDDGCIPGDPVESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZU3</accession>
    <name>T1GZU3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009400-RA">
        <property type="protein sequence ID" value="MESCA009400-PA"/>
        <property type="gene ID" value="MESCA009400"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624838_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009400-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9086" checksum="4F2C4C9C75755E86" modified="2013-10-16" version="1">MTVADEQLLASFERKILRIIWAGHHGKDSQKGQSMGYNLWSKCQPKQKRELVLFTRKLKLRALLNQVLELTISNKVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0B5</accession>
    <name>T1H0B5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vang-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Vang family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H0B5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009589-RA">
        <property type="protein sequence ID" value="MESCA009589-PA"/>
        <property type="gene ID" value="MESCA009589"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015742_2_0_1"/>
    <dbReference type="OMA" id="ESHNERF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009539">
        <property type="entry name" value="VANGL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20886">
        <property type="entry name" value="VANG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06638">
        <property type="entry name" value="Strabismus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009589-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36913" checksum="DC1C913089C3FCC5" modified="2013-10-16" version="1">CSSECKGLLVSLAARLVLLALGLWALFLRRSIATMPRIFLYRSLVILLVTVSTFAYWLFYLVQVTNGAKIVVETGGEGVDYKSLVAYATNFVDTLLFIHYIAVVLLELRHQQPAYFVKVVRSPDGKSKSYSMGQLSIQHSSHNERFYEEHEYERRVKKRRARLITAAEEAFTHIKRIHNEPSPTIPLDPHEAAQAIFPSMARALQKYLRVTRQQPRHTFESILKHLAHCLKHDLSPRAFLEPYFMESPILQNEKERRSLQSWSLVCDELLSRPVFSECTFQLIQNDVSLIVSIHKLPHFNISEEVVDPKSNKFXSKMTCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0C1</accession>
    <name>T1H0C1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0C1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009596-RA">
        <property type="protein sequence ID" value="MESCA009596-PA"/>
        <property type="gene ID" value="MESCA009596"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3413313_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009596-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="28" mass="3141" checksum="25B9C4C72462772F" modified="2013-10-16" version="1">MDFYGRNKHRSHKTGRGCFAAGRSTENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0F0</accession>
    <name>T1H0F0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139357">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0F0"/>
    <dbReference type="STRING" id="36166.T1H0F0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009626-RA">
        <property type="protein sequence ID" value="MESCA009626-PA"/>
        <property type="gene ID" value="MESCA009626"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623135_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009626-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="17982" checksum="DBB8081C7B35AE60" modified="2013-10-16" version="1">MCQCLIFLEDPLAVAEVLEKLTSSNSDQNTLMAYQIAFDMYESATQEFLGQVSQALKATAPIPGALPTTFKPQGTQASTSSTNEKEAEEKVVEEERTIDSLNDSEKAHQKNIEKLISILSGEITIDLQLQFLIRSNHADLQILRGTKEAVRVSICHTATVIANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0I6</accession>
    <name>T1H0I6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02152901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0I6"/>
    <dbReference type="STRING" id="36166.T1H0I6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009665-RA">
        <property type="protein sequence ID" value="MESCA009665-PA"/>
        <property type="gene ID" value="MESCA009665"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009665-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7699" checksum="3C4F2AF450BA9CF1" modified="2013-10-16" version="1">MSTSTINKAEMNVNAKVLALSVLGLGSLIFGLLPSCTRIGRYPLVTSVLLCFGAGVLLATSLVHMLPDTTFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H128</accession>
    <name>T1H128_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Electron transfer flavoprotein alpha/beta-subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02179149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H128"/>
    <dbReference type="STRING" id="36166.T1H128"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009879-RA">
        <property type="protein sequence ID" value="MESCA009879-PA"/>
        <property type="gene ID" value="MESCA009879"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_2_1_1"/>
    <dbReference type="OMA" id="TQFKFTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="3">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009879-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="19853" checksum="154813F6704F976D" modified="2013-10-16" version="1">MFSSSSKGLVRTNILRRLKSTLVVAEHNNETLNPVTLNTISAAKKIAGDDVTVLVAGTKCGSVSDALAKVPGVSKVLVAENEAFKGFTAESLTPLVLAAQTQFKFTHILAGATAFGKNVLPRVAAKLDVSPISEIIDVKSEDTFVRTIYAGNAVLTLKSKDPVKVITVRGTNFEAAKTEGGSAAVEQAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1R8</accession>
    <name>T1H1R8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1R8"/>
    <dbReference type="STRING" id="36166.T1H1R8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010136-RA">
        <property type="protein sequence ID" value="MESCA010136-PA"/>
        <property type="gene ID" value="MESCA010136"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2419348_0_0_1"/>
    <dbReference type="OMA" id="ELYNCEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505:SF12">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="1">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010136-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="11245" checksum="5A0591D345B32EF5" modified="2013-10-16" version="1">MSMASQVPIPSEWTREKTIQLINEYRKNRGLWDMTHEDYRKKDVKRELLHKVAQNIDNGKIPPLEIEKKFHTLRTQYHREIHRMKRSEPYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1T4</accession>
    <name>T1H1T4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047069">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1T4"/>
    <dbReference type="STRING" id="36166.T1H1T4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010152-RA">
        <property type="protein sequence ID" value="MESCA010152-PA"/>
        <property type="gene ID" value="MESCA010152"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536302_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15586">
        <property type="entry name" value="PHD_ING4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000016">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="63"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010152-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="19655" checksum="B4C83701889B5D4B" modified="2013-10-16" version="1">AKETTAKKRKANVANDVGTSGKVAGSVARKKPKKQDALEEIVSEIKASHASDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVGLTNKPKGKWFCPKCNQERKKTKILGIALLFFFQSNNCSNNIITISMKIRKHQPEVSFVARGESALERQFLQHHNQRLCIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H211</accession>
    <name>T1H211_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H211"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010240-RA">
        <property type="protein sequence ID" value="MESCA010240-PA"/>
        <property type="gene ID" value="MESCA010240"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742929_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010240-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8427" checksum="247037B25B7D653B" modified="2013-10-16" version="1">MFIDISLTYLGVEIPKYQFRRQDANLTSSSEARTNQGTLYQALPHTTKLFFYFTFTFMLTILRYPSKVLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H240</accession>
    <name>T1H240_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02094208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H240"/>
    <dbReference type="STRING" id="36166.T1H240"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010272-RA">
        <property type="protein sequence ID" value="MESCA010272-PA"/>
        <property type="gene ID" value="MESCA010272"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_4_1"/>
    <dbReference type="OMA" id="LEYEECK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF95">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588686" description="Peptidase S1 domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="28"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010272-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="20379" checksum="8D18C195F3DCAB72" modified="2013-10-16" version="1">MKSIIAIIFLACVTLAFASKIGPPKGRIVNGVEAKVGQAPYIVSISDDDGESYYHSCGGIIIDKEWILTAGHCLIESGKVRVFIYAGVTDNSNRTGGQERATDYAIIHPKYPNDGSSAPYDIALLHLEKPLEFNELVKPIALPYPAEFFSGKGKLYGWGRTDLNQPSPNILQTVESDILEYEECKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H258</accession>
    <name>T1H258_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02191765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H258"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010291-RA">
        <property type="protein sequence ID" value="MESCA010291-PA"/>
        <property type="gene ID" value="MESCA010291"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690667_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010291-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8790" checksum="7E08D2327D641F1C" modified="2013-10-16" version="1">MPRPTKERGPSRERRSGGKSPLNNFKHNFSFRKGKKAHIPMILEANLFQIKPKHGFWIELHKNNSKEHDIWMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Z5</accession>
    <name>T1H2Z5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Z5"/>
    <dbReference type="STRING" id="36166.T1H2Z5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010602-RA">
        <property type="protein sequence ID" value="MESCA010602-PA"/>
        <property type="gene ID" value="MESCA010602"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654142_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010602-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18977" checksum="883EE28EF65FC8D1" modified="2013-10-16" version="1">MLKFQKFSNIPMLEKAGELELNDVKFQVNKEGFVIVFVNSDSHHSVFFDFRHILNVEQPNNDEFATIHAINAAAKFGVKKLCIGTKSKLILEDFRNWLYNKSRKRNQYGEQLEKYMNFIEFDKILERIEIEWIDSEGYLYPWKNKKLAEESFVSQMAQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3L1</accession>
    <name>T1H3L1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3L1"/>
    <dbReference type="STRING" id="36166.T1H3L1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010840-RA">
        <property type="protein sequence ID" value="MESCA010840-PA"/>
        <property type="gene ID" value="MESCA010840"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1416633_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF98">
        <property type="entry name" value="PRE-LOLA-G, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010840-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="21200" checksum="4741A5198036FEF8" modified="2013-10-16" version="1">MYNGEVHVGHEQLPDFLKTVQLLQVRGLADVNGSNSRTTAIPSSVSSSTPTSQLASLTNSLPNPMQSSVSSVSTPAIQELKASPSPHNWESERGNHLTPPPQKRIKSADLFRAQHGISPERFVMEREFPLGQHPLTRERDRSRGERDISRETSNDRSLDLRDSLLGQALESGQGQGSYVKTEKIVYDLLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3U5</accession>
    <name>T1H3U5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02092376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3U5"/>
    <dbReference type="STRING" id="36166.T1H3U5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010934-RA">
        <property type="protein sequence ID" value="MESCA010934-PA"/>
        <property type="gene ID" value="MESCA010934"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177359_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010934-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12984" checksum="B645AC0364C29521" modified="2013-10-16" version="1">GLPSVEEKIVEDTELLKILYSYLENEPPLNPLLSSFFSKTISMLLTKTPDKDWFLYQKTCLQLLEYLKSRENFIDLIIRHFCTPVIPDLIMQMLRELQGAPLKKNLYELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H410</accession>
    <name>T1H410_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7041 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H410"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011008-RA">
        <property type="protein sequence ID" value="MESCA011008-PA"/>
        <property type="gene ID" value="MESCA011008"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888338_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055469">
        <property type="entry name" value="DUF7041"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33327">
        <property type="entry name" value="ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33327:SF3">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23055">
        <property type="entry name" value="DUF7041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7041" evidence="1">
        <location>
            <begin position="11"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011008-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7138" checksum="24367A710B9998FD" modified="2013-10-16" version="1">MNAAANIQKTPPFWKECFKLWLIKVGSIFSIAGITRDGTKYNYIVGSLDSSILQFISDKLKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H431</accession>
    <name>T1H431_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H431"/>
    <dbReference type="STRING" id="36166.T1H431"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011030-RA">
        <property type="protein sequence ID" value="MESCA011030-PA"/>
        <property type="gene ID" value="MESCA011030"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318291_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38337">
        <property type="entry name" value="AGAP010540-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38337:SF1">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011030-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="23291" checksum="36B5D4B78DE1C85E" modified="2013-10-16" version="1">MFNGIEIFQSTNGYSPISSSSDRHQDNYNYHINIFHQQQSPPPPQARQVSSVAAGASSRAISYQNMEPTTSSSLASTTTALATISSSSSSTVPNQYHAQHATNHNQYGFSSNLRYEQFTRHNTRASSLNAIGDSMSEIILDENNEPTSAILEFCKKRFLQPYVTILGLIGLNPIPTDSTVCKTFLHFLYSFVIFIVISMSYLMMYLVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4P0</accession>
    <name>T1H4P0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed DNA polymerase family B exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CAQQ02028136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4P0"/>
    <dbReference type="STRING" id="36166.T1H4P0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011256-RA">
        <property type="protein sequence ID" value="MESCA011256-PA"/>
        <property type="gene ID" value="MESCA011256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962115_0_0_1"/>
    <dbReference type="OMA" id="GALENNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="2">
        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14156" checksum="30350BE96A010725" modified="2013-10-16" version="1">MRKPIPGLDPTSCEFRGREIKRVPVIRIFGANSDGVKTCMHVHGVFPYFYIPYDSSDLENIEAVQYQVAGSIDRAINVSMGQGSSTNSHVFKVQLIGSPPLGALENNCWSIIQPFFMSNPINTFGADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5A0</accession>
    <name>T1H5A0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RSE1/DDB1/CPSF1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5A0"/>
    <dbReference type="STRING" id="36166.T1H5A0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011476-RA">
        <property type="protein sequence ID" value="MESCA011476-PA"/>
        <property type="gene ID" value="MESCA011476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1528923_0_0_1"/>
    <dbReference type="OMA" id="LCIGTNY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="20077" checksum="CB8D0C82CE498BCC" modified="2013-10-16" version="1">MVTDAEKNLIIYMYPPPPIVPECSIELDEWEHVTAFKIVSLAYEGTRSGLKEYLCIGTNYNYSEDITSRGRILIYDIVEVVPEPGKPLTKFKLKEIYKKEQKGPVSAITDCLGFLVTSVGQKVYIWQLKDGDLIGVAFIDTNIYVHQMISVKSLIVIADVYKSISILRFQEEYRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5H7</accession>
    <name>T1H5H7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02022712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5H7"/>
    <dbReference type="STRING" id="36166.T1H5H7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011554-RA">
        <property type="protein sequence ID" value="MESCA011554-PA"/>
        <property type="gene ID" value="MESCA011554"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038177_4_1_1"/>
    <dbReference type="OMA" id="GACHEEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000081">
        <property type="entry name" value="Syntaxin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011554-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12800" checksum="7782E5CD086DAF65" modified="2013-10-16" version="1">MTQQQLLLFEEENSKMAQHREEEVNKIVKSISDLHDIFKDLSHMVQEQGTVLDRIDYNVEQVQTRVSEGLRQLQRAELYQRKNRKMIAIMVLAGITLFMMTLLIFTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5I2</accession>
    <name>T1H5I2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5I2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011559-RA">
        <property type="protein sequence ID" value="MESCA011559-PA"/>
        <property type="gene ID" value="MESCA011559"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457367_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="1">
        <location>
            <begin position="14"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10803" checksum="997B02DFD30CCCB7" modified="2013-10-16" version="1">MTRFKLNQSQVTEFINMYQERECLWNQNLAIFTDSRRKHRALQEIASHFMVLHLVITTFRHMYKLISQDFENWAFICSNPSQRVSILNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5L0</accession>
    <name>T1H5L0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5L0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011591-RA">
        <property type="protein sequence ID" value="MESCA011591-PA"/>
        <property type="gene ID" value="MESCA011591"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485901_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011591-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9841" checksum="C0EA51254709FDC6" modified="2013-10-16" version="1">MFLSAISFKVLITTSWIIRRAKSNMIFVGIKFTADDSSSSSILWTIRVLFVTSVYFSIDMVFTIISTSVNFVNINFIDNITLDNTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6C6</accession>
    <name>T1H6C6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EnsemblMetazoa" id="MESCA012259-RA">
        <property type="protein sequence ID" value="MESCA012259-PA"/>
        <property type="gene ID" value="MESCA012259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504283_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9942" checksum="F46AB2F83390CED1" modified="2013-10-16" version="1">KTTHANLXXXXXXXXXXXXXXXXXXXXXXXXXXXXSNEQPNRVEIYEKTVEVLSRSKETPKLYVFSTQRLKHFLEKSDFAMLKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H751</accession>
    <name>T1H751_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exportin-1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H751"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012533-RA">
        <property type="protein sequence ID" value="MESCA012533-PA"/>
        <property type="gene ID" value="MESCA012533"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_423676_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exportin-1 C-terminal" evidence="7">
        <location>
            <begin position="285"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="618"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012533-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="647" mass="73535" checksum="DCEAEA5BAAD33E2A" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVHTFYEAVGYMISAQVDQVQQDLLIEKYMYLPNQVWDEIISRASKNVDFLKNMAAVKQLGSILKTNVAACKALGHSYVLQLGRIYLDMLNVYKIMSEYIVQAITASGVNVNNQPLIKVMHVVRKETLTLISEWVSRSNDNQMVMDNFIPPLLDAVLMDYQXXXXPLAREPKVLSTMAVIVNKLRNHITQEVPKIFDAVFECTLDMINKNFEDYPQHRTSFYELLQAVNQHCFRAFLNIPSAQFKLVFDSVVWAFKHTMRNVADMGLNILYNMLKNLELHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEVTGEDDSDLYLEEREATLREEQNNKRQMQRNIPGMLNPHEMPEDMQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H773</accession>
    <name>T1H773_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H773"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012611-RA">
        <property type="protein sequence ID" value="MESCA012611-PA"/>
        <property type="gene ID" value="MESCA012611"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_732772_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015482">
        <property type="entry name" value="Syntrophin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554:SF1">
        <property type="entry name" value="FI16515P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554">
        <property type="entry name" value="SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012611-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="378" mass="42676" checksum="4F95515FF76A73A8" modified="2013-10-16" version="1">ERMVQITRQRIGGLGLSIKGGAEHKLPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPLMMAYVTRYIYGTDKLRSNAFEVRAMNGTSSGIIHCDDLAILSQWLKLITDNIFGLTHLQMKLFNRNFAVGERIEYMGWVNEGLITSTITWQSYKPRFLILKGTEVMLFESPPSTSIIINXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWHYKFSQLRGSSDDGKSKLKLHFQDHETRAIETKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA00</accession>
    <name>T1GA00_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02121318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000042-RA">
        <property type="protein sequence ID" value="MESCA000042-PA"/>
        <property type="gene ID" value="MESCA000042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534301_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19425" checksum="87CA9F91B23D4423" modified="2013-10-16" version="1">MPAVISAIFSAIYAALAKKDDYKGTLDEIFPAMTDPQQIEAMNALSGTNATELFLGGYGRTASQQGGYQLFGIALTVAVAIVGGLITVYKGIFLYRNTPVTGDMNPKYEAKVSDPYQNVIIVHYCRKQKRALPTKQKRALPTIGLLLKTSCIRNLDKEEQHEDDQYWEVPEEKEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA98</accession>
    <name>T1GA98_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000148-RA">
        <property type="protein sequence ID" value="MESCA000148-PA"/>
        <property type="gene ID" value="MESCA000148"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443372_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10352" checksum="7F73CDC398CE251A" modified="2013-10-16" version="1">MLSRQIYSKFSLRLHRSQFSLNLEEKYFLPTARGIETFVAQDISYVDPAFLKVRVNSIHTGTGEVVGQKTSKGDQLICKKNYLPFVRLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAL6</accession>
    <name>T1GAL6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAL6"/>
    <dbReference type="STRING" id="36166.T1GAL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000275-RA">
        <property type="protein sequence ID" value="MESCA000275-PA"/>
        <property type="gene ID" value="MESCA000275"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_318656_0_0_1"/>
    <dbReference type="OMA" id="HINATPE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000275-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="913" mass="102512" checksum="D96761AE7A084B49" modified="2013-10-16" version="1">MSCESDFIKSRTQNLSSASKTYRNFKKRIIQQNKFNSIQPYSDWSKKFYMMQSELPEIFMEITSTGKSKNKIGKVIPHKANKDLRNSTSMGNSSNSGNTNSSNSNEHEHIFDKSLKSIKLAKLLTVIRGLVSLLLEMDFTCNMDLFLLTCKVIARLVSVCRPSVQLSKIITSSQLEQLIRLAVWNDQQQPWAIHAITCLLEDLLDADKNFKESGDNMSESSECKEHKPMETEEQQQKQQISQPIFNYTEMDEIAAEIEILSRCKPKKDATNTTITLNKNSLNYFCKSVSCAMDSRLEIGLNTNIEINICRLTMTSSLDMYSSLPQIPTNEHINATPELLNWSDSLTNYWSNSEYNSGISTYDMFKNVFDSILMDLNIQNSWIHLENVLQLWLSLNSELSERSYQSRFFSRMDIPKIEIGANAIKGLLSALSWHCDIKSRTWCLSFECLILACNTYLDKLANGAPIDDIPKIENIINDENFEKMLLRFFSGYGMGSNMLTNRCAGPTISKHLHILLMNLQMGTLLTFNNIVNSDKVKCQRACDTNGGSNSFGSLFATVLGSDNSKQTHPVSDNAILISLLTLSISLARTEVSKISDDKIVCDDFMSTNSQSDEIKIERDIQDYKKPKCIADFVLLQSTTMNRLLSSLSCCSNSSVLLNSLSHTVDHNSIFEAETVADALYTLLIVLADKASDQSLIVEPLYAFLESNVSSRNAMPKLQISEPFLWYILKVLEVPGAVKVFTKLGGIQILCQNIVKSNKVIVNLQPGLISLIMQHMTKYSQLKLNNFASSSSSSSSSKKSSSNQMQKNQDGLINFAPFCTISCEKTAAQPADVLIQAPVASQRRARTPAWSYMFYPNESWVELVITLPSAILLKEVQIQPHISTLATCPSAVALEISRDFSLGSYQLDKHYLRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAY0</accession>
    <name>T1GAY0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAY0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000397-RA">
        <property type="protein sequence ID" value="MESCA000397-PA"/>
        <property type="gene ID" value="MESCA000397"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963442_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000397-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6702" checksum="6C6F46EAE2290470" modified="2013-10-16" version="1">MIANRNCGSVSEDSLHMTSMPTAFSKIWLYLFSLIVDAECWKVGRSHDHFQEDVEIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAY4</accession>
    <name>T1GAY4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02135700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02135701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAY4"/>
    <dbReference type="STRING" id="36166.T1GAY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000401-RA">
        <property type="protein sequence ID" value="MESCA000401-PA"/>
        <property type="gene ID" value="MESCA000401"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1637357_0_0_1"/>
    <dbReference type="OMA" id="TTLICEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF197">
        <property type="entry name" value="FI04477P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000401-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18949" checksum="43F624CB6F2BB0D1" modified="2013-10-16" version="1">MKFQIFNITSKKKFCRVATDWALVLSMSLIILILELFIQPYQRGFFCDDENLRYPVKPNTYPAWSLGVITAIPVVIICVTEYLIDRCSLKSFCLQIYKQIGVFMFGFLVHFLLVDYIKYCGGRLRPHFYELCQPVFPDGKRQSNRNFFGVIQIIMMTSDLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB25</accession>
    <name>T1GB25_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02028452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028453">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000445-RA">
        <property type="protein sequence ID" value="MESCA000445-PA"/>
        <property type="gene ID" value="MESCA000445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888357_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7215" checksum="CE5CF7915F32CCB9" modified="2013-10-16" version="1">METEDLVVVAKHTELVKKSLKSKCLEELCQPSGICLKVRRCLRVLQKHSTTVVLNFGKYLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCY5</accession>
    <name>T1GCY5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02141650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCY5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001158-RA">
        <property type="protein sequence ID" value="MESCA001158-PA"/>
        <property type="gene ID" value="MESCA001158"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3406724_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001158-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="30" mass="3649" checksum="733EF181EF20A96E" modified="2013-10-16" version="1">MIKSDFSNLFCLCFNYSLREMEPKRVLLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD81</accession>
    <name>T1GD81_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the muscleblind family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02063837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD81"/>
    <dbReference type="STRING" id="36166.T1GD81"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001262-RA">
        <property type="protein sequence ID" value="MESCA001262-PA"/>
        <property type="gene ID" value="MESCA001262"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191707_0_0_1"/>
    <dbReference type="OMA" id="RPINSAF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF12">
        <property type="entry name" value="PROTEIN MUSCLEBLIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001262-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7348" checksum="3109037CD5244019" modified="2013-10-16" version="1">MAMISMNNLLNGKDSRWLQLEVCREFQRNRCTRQDTECKFAHPPTNVEVQNGKVTACYDSIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDI4</accession>
    <name>T1GDI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02006007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006008">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006009">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDI4"/>
    <dbReference type="STRING" id="36166.T1GDI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001372-RA">
        <property type="protein sequence ID" value="MESCA001372-PA"/>
        <property type="gene ID" value="MESCA001372"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388465_0_0_1"/>
    <dbReference type="OMA" id="TECIEYS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000093">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001132">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001715">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001849">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF6">
        <property type="entry name" value="CONTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="101"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="204"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="382"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="534"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="622"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="633"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001372-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="711" mass="78159" checksum="C764E7379E3C6B52" modified="2013-10-16" version="1">MTKVGAGPTKTATIQSGVEPVLPHPPTSLALSNIEPFSVVLQFTPGLMVTHQSFGGQLKVKHQEMSLGSQLRLIATNVAGSSEPSEPTKEFQTIQARPMHAPFNVTVRAMSATELRVRWIPLQQSEWFGNPRGYNITYRQAENDPKLPAMSVIIDDHTANSHVLDGLEEWTLYEITMKACNDVGNSVSSPKAIERTREAVPSFGPIGVQANATSSTTIVVRWSEVPKEHRNGQIEGYKVFYAASSRGGLVLHKTISNNASFTTTLTELKKFVVYHIQVLAFTRLGDGVLSSPPVRVQTFEDTPGAPSNVSFPDVSFSTARIIWDVPEDPNGEILAFQVTYSLNGNRTFRASLQSVTAQTRLGWGKTATALVYTTNNRERPQPPSPPQISRSQIQAHQITFSWTPGRDGFAPLRYYTVQMRENDGPWTSLPERVDPSAASYTATSLRPHTTYQFRIQATNDLGPSAFSRESITVRTLPAAPMEGVSGLKVVPITTTSVRVDWNPLEESVWNGDAATGPATPPVAVYVGEAVPTGEPRSVEAAPISSTEVRLRWKPPKQNMQNGDLLGYKIFYLVTDSPQELEEGRKYEEEIEVVPASTTAHSLVFLDKFTEYKIQILAFNPAGDGPRSPPITHSESENESVRSDPHSFVNHYANVNDSLRQSWKKTKPMRNYSSYTDSEPEGSGVMSLNGGQIIVNNMARSRAPLPGFSSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDU3</accession>
    <name>T1GDU3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043391">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001493-RA">
        <property type="protein sequence ID" value="MESCA001493-PA"/>
        <property type="gene ID" value="MESCA001493"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1099567_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001493-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="253" mass="29758" checksum="B236C2AA31CE1C6E" modified="2013-10-16" version="1">MEGLIIEPQLSSLNAKNYLRKFTTLEQVFGNQKNLESLNISNSEINGNFLEVLSQNKYLTHLKFSNCSLASDAVNFRFVQRLTNLTLNWSDNLMCACWKHLMKLEYLHLAHNESNSKDLSLELDFVYLPRLKHLEIANDIYTNLIVRQLIAPNLSFCCTGFKVCKNIPQTKVLKIKDFMDKNKSKELFKALKEQNPTLDELILSYQWINVDLLKMYLKSELDIKTLRVELFFWKSSSMYSSMWKSHKLKRIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDZ9</accession>
    <name>T1GDZ9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDZ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001554-RA">
        <property type="protein sequence ID" value="MESCA001554-PA"/>
        <property type="gene ID" value="MESCA001554"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340506_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001554-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23105" checksum="D528CD737920355B" modified="2013-10-16" version="1">SDQQIINNIQKNNTTVKKVPNVTTKIHQEQNGQSLQQKEINVNKNPTSPNVKKVINNLPNSQNPLKTSILQSIQPQVSNVNKNAPKNDNVPFCSKRRIYKNHQVYVKLPQATNVKNSSIQIQDTPTMMKTISTNASKESNVHLNCPKPSVLLSPNQNIGLEQGSLYGDGCKNSCICTSKKNLSEYLKNTFEFMTNHWTCEEKEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE34</accession>
    <name>T1GE34_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE34"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001592-RA">
        <property type="protein sequence ID" value="MESCA001592-PA"/>
        <property type="gene ID" value="MESCA001592"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592514_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001592-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9519" checksum="60DEE9EB7E88C2C7" modified="2013-10-16" version="1">MEAMCIRTSITEVIGLQKQQRGSGWKDQEYNETVSMKDKLYRRSIQIISRATKEAYRSKRREVVKMEIEKETLRKTTSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEJ2</accession>
    <name>T1GEJ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02001912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEJ2"/>
    <dbReference type="STRING" id="36166.T1GEJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001756-RA">
        <property type="protein sequence ID" value="MESCA001756-PA"/>
        <property type="gene ID" value="MESCA001756"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019279_2_0_1"/>
    <dbReference type="OMA" id="RDHETHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001123">
        <property type="entry name" value="CK1/CK1/CK1-D protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001756-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="23706" checksum="CDDE0D6E371FC4F3" modified="2013-10-16" version="1">MIGRLEFIHIKCFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKFRDPRTRQHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGILPWQGMKANNKKQKYEKISEKKMSTPIEVLCKGFPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRMYFVLRDFEMILPFEDRSLLNRKFEKQRYKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF46</accession>
    <name>T1GF46_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF46"/>
    <dbReference type="STRING" id="36166.T1GF46"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001975-RA">
        <property type="protein sequence ID" value="MESCA001975-PA"/>
        <property type="gene ID" value="MESCA001975"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002568_1_0_1"/>
    <dbReference type="OMA" id="CFHGWSA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11280">
        <property type="entry name" value="gelsolin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000033">
        <property type="entry name" value="FLII, actin remodeling protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000020">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000031">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF51">
        <property type="entry name" value="PROTEIN FLIGHTLESS-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="187"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="4">
        <location>
            <begin position="524"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="4">
        <location>
            <begin position="625"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001975-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="973" mass="111268" checksum="F71AEC748BAAA151" modified="2013-10-16" version="1">MANTGVLPFIRGINFTKNDFSDDKFPEAIKLMTRVQWLTLDRTNLTEVPEQMGALNKLEHLSLKHNELEKLFGELTELSCLRTLNLRDNQIKSSGIPPELFKLDELTTLDFSHNKLKEVPEGLDKAKNLLVLNLSHNQIESIPTALFIHLTDLLFLDLSHNKLETLPPQTRRLANLQTLNLSNNPLELFQLRQLPSLQKNEINEISTSVENWQKLETFNLSRNELTNLPSALCKLGNLRRLFVNDNQLNFEGIPSGIGKLGSLEVFSAANNKLEMVPEGLCRCGSLKKLNLSSNRLITLPDAIHLLEDLDQLDLRNNPDLIMPPKPTDSAMKGAGVEFYNIDFSLQTQLRLAGANVPASIPQAQTQKSDPIARKIRLRRGPRNDADQDQAKILKGMKDIAKEKDAAADDSFENGFGKPESLKPKRWDESLEKPPLDYSKFFEKDDGQLPGLTIWEIENFLPNKIDEVTHGKFYEGDESDEFLALFDTELTYIEGGRTATGFYTVDDMIYTTRCYRVHSASTSIHMEPVGVEFASLDPRYVFVVDCGLKIYVWYGRCSKNTLKSKSRLLTEKINKIERKNKADLMIENQGEETEEFWKHVDENFTPHLPRLYQVQLGMGYLELPQIEIPEQKLTQSLLNSKNVYILDCYSDVFVWFGKKSTRLVRAAAIKLSRELFSMINRPEYAMVTRINEDTETQIFKSKFVGWDEVMAVDFTRTATSVAKTGADLTKWAKQQETKADLAALFMPRQQAMSLQEAEQFEEEWNYDLEAMESFVLEGKKFVRLPEEELGHFYTGECYVFLCRYCIPPEEDEENPEDESKRPPQDDEIQCVVYFWQGRDAGNMGWLNFTFTLQKKFKVMFGEELEKGQSVEICRHKAPVEFYHLRSNGGALCTRLIQISPDASHLNSAFCYILFVPFETEDEAQSGIVYVWIGSKAAPEESKLIQEIAEEMFNNPWVSLQNPANNSMSSRVHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFJ9</accession>
    <name>T1GFJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFJ9"/>
    <dbReference type="STRING" id="36166.T1GFJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002143-RA">
        <property type="protein sequence ID" value="MESCA002143-PA"/>
        <property type="gene ID" value="MESCA002143"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="OMA" id="KWTFSFW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000042">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF93">
        <property type="entry name" value="CYTOCHROME P450 310A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="53958" checksum="FF181041645D40CF" modified="2013-10-16" version="1">MFIPLLLGAVTLLIYFVWKTFKHWDNEGIPFDKPSIPFGSLKPLIKKERSFGMAIYDVYTKYKEPFLGIYLMFRPAILVRDATLCKDVLTKDFANFHDRGVYVDEEKDPFSGNLFSAEGQSWKSLRTKLTPSFTSGKLKAMYPTILETADKLTNSLNEKLPKGDSKIIEIKDLMNSFAIDIVASVIFGIEIDSFKNPNNEFRIAAYHANAPDFLSNMRGAVLFLYPKMEKVFRFFRVPNITADFMKKIVNADDDNWKFQTVAENLKSMNMEKLSGQGFVFFAAGFETTSATASYCLYELTQNPELKVRVVNEINEVLTKYNGQLTYEGLHEMKLLDRCVMETNRKYPGLPILNRICTQDYKVPGSKYTIKKGTPIVISLMGLHRDPEYFPNPMKFDPDRFENGNYNQIAYMPFGEGPRNCIAFRMGKVSAKTAIVTVLSNFDIECSEKKELEIDTHGIGILPKGGVNIKFTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFV2</accession>
    <name>T1GFV2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFV2"/>
    <dbReference type="STRING" id="36166.T1GFV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002252-RA">
        <property type="protein sequence ID" value="MESCA002252-PA"/>
        <property type="gene ID" value="MESCA002252"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608766_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002252-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8891" checksum="1F513FB85A683165" modified="2013-10-16" version="1">MNKPKITFGKIGFAPKPTETKSEDSKDEPETSGFKKFGKEEHQKKVEEVSEELEQQSLKDVMGITGFGKKAKVFDIKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG67</accession>
    <name>T1GG67_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG67"/>
    <dbReference type="STRING" id="36166.T1GG67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002377-RA">
        <property type="protein sequence ID" value="MESCA002377-PA"/>
        <property type="gene ID" value="MESCA002377"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388722_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052227">
        <property type="entry name" value="Arf-Rho-GAP_ANK-PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 15B, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899:SF2">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 15B, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002377-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10995" checksum="221B32E697ACA558" modified="2013-10-16" version="1">MKKEKNGSNTIVKEIFLHNTTVYLGCEKKRDCPWSWSLTFIENLNKDIVRTRETPFVGHVVAGSEWADRIMWFASIWYNFYGENALPPAEIILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHD8</accession>
    <name>T1GHD8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Envelope fusion protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002833-RA">
        <property type="protein sequence ID" value="MESCA002833-PA"/>
        <property type="gene ID" value="MESCA002833"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_483397_0_0_1"/>
    <dbReference type="OMA" id="YIDSCEG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022048">
        <property type="entry name" value="Envelope_fusion-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12259">
        <property type="entry name" value="Baculo_F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588290" description="Envelope fusion protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002833-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="67147" checksum="B9DF186C3F56E110" modified="2013-10-16" version="1">MMKCFLIALQITASMAVIQPQSSSGIFYEYLGTVKPYHDEASLKFYINLTEYRVLFENISQQSSETKMLCNKIDELFCKYEICSKPKKSYNCNQFLEDIFRDLKIMNESLSFFTPIEPANQRSKRSLLDGASSLLKFVIGTPTEEDTAYYSEQIKTLQGRSKILNEVNDKTSTLLSEIFETYSNLTKNLQNNLEVALSHNFGEMEKFVNKTIDREKIIQKSTELDSKMKFLSSHVGQMRSQLSKILNALSFAENGVLHTPLITPKQFLELLKESELPIVIDESFELFQVYSFPIIAIDGNVAKIDPSNKYYIFSKDNSEFSPLDLEKCKLLVDNNWVCLLKEMTSNSNDSDICEVALRNGDASKCNMLLLKINLFIVFPLQNNEWLIMTTNAENYIDSCEGKGKINPGNYKVSLKSGCSFKIGKKELLATNIIGKEKVFHEFPEVEILDKSNFTKYLRDNIKEFSFENITLIENIKSWEDLSKNITKAIQDRKEIQVHYAKQEVWKMVIISLFGSLILISVIYFVIKPIYKRRKELQKSKLNYKEQLLENTNSINSKEKLEENVKNKGDLLTILKKEKDIALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI65</accession>
    <name>T1GI65_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003133-RA">
        <property type="protein sequence ID" value="MESCA003133-PA"/>
        <property type="gene ID" value="MESCA003133"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725082_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003133-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8156" checksum="43464EEAB396FEA8" modified="2013-10-16" version="1">MYLEPTVAYTGGGVQDFRAVLIEYKADISALQEIRWTGEVPSSPTSAYTKKHGVHPDQCQSSWYDYAEFSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIE4</accession>
    <name>T1GIE4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02057161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIE4"/>
    <dbReference type="STRING" id="36166.T1GIE4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003218-RA">
        <property type="protein sequence ID" value="MESCA003218-PA"/>
        <property type="gene ID" value="MESCA003218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024840_3_1_1"/>
    <dbReference type="OMA" id="YYHPQRN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF7">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="292" mass="33752" checksum="B7700FF3A00B4EFB" modified="2013-10-16" version="1">LISVWCVNEWRKIGSPSPFQLIELGPGRGTLMADVLKVLSHFKLNKNMSIHFVEVSPYLSKLQAQKLCYKHSEISSQDSPHYREGETISGSKIFWYNRIEDVPQEFSIVLAHEFFDALPTHKLQKDSNGLWKEILVDINPERIEELRFVISKSQTPISKIFKPYKDDLRDCLEYNIETERIVRHLATRFERDGGFGLFMDYGHFGEKNDTFRAFKKHKLHDPLVEPGSADLTSDVDFKHIKQVAEEDGKLITFGPVEQGTFLNNMEGPVRLQILLEILYQKIKQISKVAIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIW4</accession>
    <name>T1GIW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003400-RA">
        <property type="protein sequence ID" value="MESCA003400-PA"/>
        <property type="gene ID" value="MESCA003400"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006528_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003400-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="14524" checksum="166F0326DE8FB611" modified="2013-10-16" version="1">MKGTLTYGIKLILQGSLFEDKLLASGEVYTPTRELEIHIIIRDSRIISSFKLYKFLISKLVFDVLCYVLNRGSKGFSDMVNSMLTSRIVISKIEWTYEFLVWWQALRKKRENCKKVEVVTPKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ61</accession>
    <name>T1GJ61_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ61"/>
    <dbReference type="STRING" id="36166.T1GJ61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003504-RA">
        <property type="protein sequence ID" value="MESCA003504-PA"/>
        <property type="gene ID" value="MESCA003504"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_11_1_1"/>
    <dbReference type="OMA" id="RIRNICY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="26"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003504-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23985" checksum="D21535B468D60668" modified="2013-10-16" version="1">MLFHSSDHGDNLSAALYALKTKKQFCDITIITDEGELIAAHKNVLSSTCLYFEAMLNGNFKDGASNTVRIRNICYQTLHECINFLYTGDISITEDNVFELLESSVFLQIEWIKEKCCSYLIRIMTVDNCLDILNIAENFSCDNLYQSAFIYVARNFSRIILKPGFLNISLKLVSKAFFICTNFFINILYKFKKNKKIFFIIFYRILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJI3</accession>
    <name>T1GJI3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L27 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJI3"/>
    <dbReference type="STRING" id="36166.T1GJI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003632-RA">
        <property type="protein sequence ID" value="MESCA003632-PA"/>
        <property type="gene ID" value="MESCA003632"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712095_0_0_1"/>
    <dbReference type="OMA" id="MVMIGSK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.470">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015143">
        <property type="entry name" value="L27_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09058">
        <property type="entry name" value="L27_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L27" evidence="1">
        <location>
            <begin position="36"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003632-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9557" checksum="51EA289B6D1E6386" modified="2013-10-16" version="1">MVMIGSKRRKDSSTLPELVDLLMIMLIFADTIINSSWLKAHRALELLEDYHSRLSDPSDRALRMAIERVIRIFKSRLFQALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP0</accession>
    <name>T1GJP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02030518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP0"/>
    <dbReference type="STRING" id="36166.T1GJP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003690-RA">
        <property type="protein sequence ID" value="MESCA003690-PA"/>
        <property type="gene ID" value="MESCA003690"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_353466_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000036">
        <property type="entry name" value="Trypsin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF95">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588340" description="Peptidase S1 domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="28"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="293"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003690-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="678" mass="73612" checksum="890851A4EE281936" modified="2013-10-16" version="1">MKSIITILLLACATIAFSSKIGPPKGRIVNGVEAKVGQAPYIVSISEDGESYYHSCGGSIIDKEWILTAGHCLIESGKVRVFIYAGITDNSNRTGGQDYAIIHPKYPNDALLLPTILLCCTWRNHWNSINWKIVWMGKTDLNQPSPDIMQTVESDILEYEEFCRGDSGGPFVKEPPNGVTQLVGITSWAYTCGKDNYPSAHTKVASHDQQLSACNGDSGGPFVKEDKNGVTVQVGVTSWVYTPCGEHNFPSAYTKVSAFIEWIAEAKAEYYKNIKFCNSFTFSLKAVPQKGRIVNGVNAVEGQAPFIVSLSCDDDPTFYHNCGGSIIDKEWILTGAHCLSSSGKQKLRRNCFVAFRETIKIQQIGQVYSSAISRRILYCHYCGGSIISKQWILTAAHCLTGSGPVRRAVFAGITNVADRAKGQEVIANYAYAHEKYSGGVGPYDIGLLHLETPLVFNDIVKPIALPYKSEIVEGEASLYGWGQTNSSLPSPDPLQRVDTKVIEFKECKAALPSGANIHESNVCSSSLNSKVSACNGDSGGPFVKEDKNGVIQQIGIVSWGYVPCGEANLPSVYTRVSSYTEWIVQPIALPYETEVVEGKGSLYGWGRTNSSLPVPEPLQRVDTNIMQFKECKAALPSSAPIHEVNVCSSSLNAQVSACNGDSGGPFVKEDKNGVTQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKP2</accession>
    <name>T1GKP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02125998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKP2"/>
    <dbReference type="STRING" id="36166.T1GKP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004069-RA">
        <property type="protein sequence ID" value="MESCA004069-PA"/>
        <property type="gene ID" value="MESCA004069"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023263_0_0_1"/>
    <dbReference type="OMA" id="CVFRNES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577453" description="LRRCT domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004069-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="56704" checksum="D627A7FB3E97A259" modified="2013-10-16" version="1">MILKYLLVIFCTFQAILSAGIRSLLESCPNECACGNTFFHDFSVSRWIIGINEPTSILESENVPMDRHPNSVHHESEPFDDNEVFYANCIIQDSTVISELIGNLSTNLEALIFLSTNEDVNLTLSSSLLKPLTFMNTLEIRSKIQGRLTFIIDEPLPQLENANFEFIVIDNTARKLNLSFEKFEEIKHYDEYILERKKPTRKPPSFSGWDNLKILRIYKSNLETLQWEHFEGLQNLQHLSLERNNIKIITEFALFGALNLKTLSLANNEILDLHYRALAGLLELKTLDLSNNKMVHLSEYTFPPFPKLEYVNLKGNPIRYLFPTTFIVMNQTKQMSLDSSTDMSLMFEVPYYEEMWLFQDLKLLQTLSLSRIDLGVLDDSIFRHLEDLKVLEINHSKIKRITFDAFTELTELRELYLSTCSIEELSMDTFNGLENIEIIDLSHNKLKYIPPGLFSGLPKIREIFLNNNKLKYLPNEFFTVVSQSLKCYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL32</accession>
    <name>T1GL32_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphofructokinase</fullName>
            <ecNumber evidence="4">2.7.1.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02160132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL32"/>
    <dbReference type="STRING" id="36166.T1GL32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004218-RA">
        <property type="protein sequence ID" value="MESCA004218-PA"/>
        <property type="gene ID" value="MESCA004218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020655_3_0_1"/>
    <dbReference type="OMA" id="YGHERFA"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="361" mass="40100" checksum="A10B714C41A421FA" modified="2013-10-16" version="1">MHIGAPACGMNAAVRSFVRNAIYRGDTVFGIHDGIQGLVAGNIKEMGWSDVTGWVGQGGAFLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGFEAYHACGQISDQRSNFPEFCIPMCVVPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVQRGLILRNEKASENYNTDFIHRLYSEEGGSPTPFDRNMGTKMAAKGVEWLVAQCKQNMQPDGTVKCTSNESACLLGIVRRQYRFTPLVDLISETNFEQRIPKTQWWLKLRPLLRILAKHDSAYEEEGMYITVEEEQKTDAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL48</accession>
    <name>T1GL48_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL48"/>
    <dbReference type="STRING" id="36166.T1GL48"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004237-RA">
        <property type="protein sequence ID" value="MESCA004237-PA"/>
        <property type="gene ID" value="MESCA004237"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1306124_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052118">
        <property type="entry name" value="Rho-GAP_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 100F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150:SF3">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 100F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004237-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23716" checksum="C20863D09E3ACEBF" modified="2013-10-16" version="1">MEIVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGGRGTSSPAPTTLSRPEQKPPPVVVIKRDLREDEMDDPERIPRSRSSRERRTGDGREMTESRSRLGLGLNNYSPQSEQLDLYYNNRPNALNDPPSWGYKPPPPPPPVITEQPKPHTFAPSHSYYQNAGTLESLAEKVHSFYPGTTRRMSAGTAGVGFGQQQHHMRFPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLC6</accession>
    <name>T1GLC6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004326-RA">
        <property type="protein sequence ID" value="MESCA004326-PA"/>
        <property type="gene ID" value="MESCA004326"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834092_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004326-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7651" checksum="C1C42E24D7BB5901" modified="2013-10-16" version="1">MRQNSVLGTIVIFLRGNNWIPNAGNLYGIFTKSSKGIFIHYTVHYEILCYFCIECITFTQKDETKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLD7</accession>
    <name>T1GLD7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLD7"/>
    <dbReference type="STRING" id="36166.T1GLD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004337-RA">
        <property type="protein sequence ID" value="MESCA004337-PA"/>
        <property type="gene ID" value="MESCA004337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015992_0_0_1"/>
    <dbReference type="OMA" id="ANKYFRH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21675">
        <property type="entry name" value="SMP_TEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="9">
        <location>
            <begin position="322"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="625" mass="70543" checksum="97F7949868C48095" modified="2013-10-16" version="1">MNEINNYDPNNYHISMTRSVYIKLEGSSLRIANTTARIPKREMWNEVPTDINKLIFTNIRTYNLLGCKIEMLPRGLARKRYFGRKYPIQLIIKNNNNSRNSVDQNIPTETYNSDNGFVELSLPKTEKPKKMSAGKIDILLGDGDFSESSKKSDNDNVMDFGAVVLNSDTTPNIESEEPNIPCGDETRLLLFARGDREKEDWYRRFLAASQGHVEDRDVRLPNVTFVDDSEVKAAAKAATFIPDNEKSVDNSSPNKTPDEKLRRLILQTRQKSTKRTKACSKADDSIPLVRKTLVPSDSKSRREKRQKDELWKGIDQSLFLGPCGSVVWANVLVGRILYSCLNDALLQSKIQEFLQKKLSAIRLPGFMEDVSITQIYLGETPPIIHRVSQPVLDERGTWIDADLTYEGLMHMTITTKLNLLRLKRQHSRNNMDSVGASGTPINEQTQTLDDLSSVLDPSFANCAIYDSNAESSGASSSESESPTYAAVDANTEQHTNPGNARKIFKIVDRITASNIFQYATELSYVQRAMENMSTNITLRVHLKGLVSRVAINIPPPPSDRIWLAFRGPPRLWISAKPAVGDHTFDWSMITNVIESKLCDEVYKYLVYPNMVDIIVPFLGQSTYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLP1</accession>
    <name>T1GLP1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA004454-RA">
        <property type="protein sequence ID" value="MESCA004454-PA"/>
        <property type="gene ID" value="MESCA004454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_258576_0_0_1"/>
    <dbReference type="OMA" id="RICERNQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="791"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="865"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1338" mass="155484" checksum="1924BFD6F2A070C2" modified="2013-10-16" version="1">MDEIVKKDSQFNEESTETKNDEIENDEKEKHSLQEQSGESEAKKLGNEADLSGNKSLNGSDNKPKILDVTVIQVSQHEVPTPSEAENMENTNPSEKAEIKISAEKVPDQQPEISKSPVTGKAQENTHQIESNNISLTQNPIPMEVNTFGNENSKEAEIIDVDLEDDSNEKEQTTHSSKNENNEAPKNSNNEDIPKDQPNSSMEVEKSFNEIQAFIDLVETGEYDNLVEKTSQHALAKDESKEPKNAVSKIFKSPIKKPVMEVIKTADFIEVLSDDDDDEEPTETSVVEPEKQTSFLRVHDIPFKEKNQNETESFTEQASLNEHNMNISKIDESAQDETLMVIEVPPSNNDNKSEEILSIDDDEEQKFSEEKNPKETKDLENEIDSESKNDDEHGSSNKDEVPSKLLQDLIENPPKVTTEPIVGNFGRHFPPIAVQNVEGLKTDFKVEKHKPVKTKLVIQRNLGDVILDQEEMKRLKRERMKEFMRLKDKERAYNSQKFRKKSPTSNEKPAKKDLLLNPFAIIPKFDSVKKPPSTTSKSKSPLSYDLTDDDAVISSNEIEKNLSKIRDSVTATKRSTTSLPTSNHFPTNTNKPERKRSRFSSPTLPSTSQEPPSKSSTISVFDIINDVSKDMRRNRPSEPSHRRESPPRLNRNERFGDARMGRSSRERKRSPSPRTEFKLTANRIERNIRQQKQANSDSRRDVDFREMRFSVKNDLSLEERERMREHLERERMDEEHHAPPSPPRSAFREDFDHRHHPSEFPGRREFYPRDFPVRDERDEFMDRNRRFDSDYRPEYSRHSPEMRDFPDRRNDFPTRRFDRSPERDDFNYRNRFSREHDFHPRNRSRSPRDFDRRDIGNMNNFTLQRRFPSNSPPRRFSIPRDDDMVQQNINRFENNRRPGDMVPQNINRFEDNRRYGYGDIGNQNINRFEDNRTPVENLNRFDDNRRNNFEDRGSMFKVSNPRNIENFEMTPNMLQSSSPRMVPPPADFDLDERSNPNKPSIFKRLNSANRTPSPPPPPIYNPEDILTSRSQSVSPQIPNFNNVGQNMQPFDWDRSNTVQQFEEEDYLIIVYCEDERQFLTPQNCSQILDHLEMLINFKFNSTAYKDECLFIQTSKSKGDWIIKVLKSCNINFCPKLTAEVCIGALPSSDSYQVSVKIVSSLMNNPLKASNFFYVYELMNNIIGQHPNWSQAINKNRVTFYKNSINIFCDQNLDLAKSLKDVLSTIRVETNMCPKLAIYIDGDFVDMTMVLDAQLKKVGVIMNAIRSVYPQFNVNSWIISSINFTSGLFHLKVPVRDVTLLCKQKLVLNIKWQQNCRGRLVFEFIDQRIERRILKNMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLR5</accession>
    <name>T1GLR5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLR5"/>
    <dbReference type="STRING" id="36166.T1GLR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004478-RA">
        <property type="protein sequence ID" value="MESCA004478-PA"/>
        <property type="gene ID" value="MESCA004478"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_1_1"/>
    <dbReference type="OMA" id="YELLHCW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000182">
        <property type="entry name" value="Cytochrome P450 3A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF187">
        <property type="entry name" value="CYTOCHROME P450 4AE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577475" description="Cytochrome P450" evidence="14">
        <location>
            <begin position="19"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004478-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="56723" checksum="A991F5FF2685945B" modified="2013-10-16" version="1">MFMIIEFLILVLTVALLADYAVKKRRTDIVKDIPGPDTLPIFGNALMYYGKSLESIMGFIQANREKYGPFYRVWIFNQLAIFSSDPRDVEVVLSSQKLITKNRLYDLLLPWLGTGLLMSTDKKWHSRRKIITPTFHFTILEQFVEVFNQQGDIMIEKLKAKADGKTVINIQPDICLAALDIIAETAMGTKIHAQTDANCQYARAVNDTGSILALRFVNVWFRQDFVFRLFSPTLFKRLKDDIKLMHDFTEKIIKERRDTLVKEQHATSLTSLDDDNKDTDLDVAVGSRRKMALLDVLLQSTIDGKPLTNEDIREEVDTFMFEGHDTTTSGLSFALHEISRHADVQQKIYEEIVEKLGADKTSSMSLRDLGELKYMECVIKEALRMYPPVPIIGRYFYEDVNMNGKIIPRGTNYTVGIFTMLRDPEHFPDPHSFKPERHLLENLDEKANPYAYVPFSAGPRNCIGQKFAMLEMKSTLAKIIRNFELLPLGETVKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLS1</accession>
    <name>T1GLS1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLS1"/>
    <dbReference type="STRING" id="36166.T1GLS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004484-RA">
        <property type="protein sequence ID" value="MESCA004484-PA"/>
        <property type="gene ID" value="MESCA004484"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2967785_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004484-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="58" mass="6195" checksum="FF82412DA68BCE2F" modified="2013-10-16" version="1">MTAAAKNSMSDWTKSRKELNNKEIEKMTDNASKFLGGVLGDISSRSAYTQILLGAASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GME3</accession>
    <name>T1GME3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02013248">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GME3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004715-RA">
        <property type="protein sequence ID" value="MESCA004715-PA"/>
        <property type="gene ID" value="MESCA004715"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608767_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004715-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8535" checksum="2EA362C235862AD5" modified="2013-10-16" version="1">MGDKYAKLKNDSVAVFEQIRKSCQAIKNSGSKMEYSDIPEPEPKKAEAKNVASSSRAVSFMADSSKSKSMSSLLLTTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GME7</accession>
    <name>T1GME7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GME7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004721-RA">
        <property type="protein sequence ID" value="MESCA004721-PA"/>
        <property type="gene ID" value="MESCA004721"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197018_0_0_1"/>
    <dbReference type="OMA" id="IRVIEYM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2080.10">
        <property type="entry name" value="Insect odorant-binding protein A10/Ejaculatory bulb-specific protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005055">
        <property type="entry name" value="A10/PebIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036682">
        <property type="entry name" value="OS_D_A10/PebIII_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11257">
        <property type="entry name" value="CHEMOSENSORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11257:SF12">
        <property type="entry name" value="EJACULATORY BULB-SPECIFIC PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03392">
        <property type="entry name" value="OS-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100910">
        <property type="entry name" value="Chemosensory protein Csp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004721-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9061" checksum="8A0CA7BCA5A160A5" modified="2013-10-16" version="1">MDQGKCTPDGRELKKNLPDALQTECSKCSDKQKEGTEQVLKYVIKNKPKEWEALQAKYDPEHIYFKKYEAEGKAKGIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMN5</accession>
    <name>T1GMN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5'-AMP-activated protein kinase subunit beta-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02065119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMN5"/>
    <dbReference type="STRING" id="36166.T1GMN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004813-RA">
        <property type="protein sequence ID" value="MESCA004813-PA"/>
        <property type="gene ID" value="MESCA004813"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070949_2_1_1"/>
    <dbReference type="OMA" id="SMSRERH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000139">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="9">
        <location>
            <begin position="75"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004813-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="225" mass="25176" checksum="144AA944E254CEE5" modified="2013-10-16" version="1">MRQSQEDDEEPYYTKPVSSVSTNIGSNTTSNNQSLEKVPRQRSSTISEGTNKRPSIESMSSSADDSAVPQNDALPTVFKWDGGGKQVFISGTFSNWKALPMVKSHKDFVTIVDLPEGDHQYKFFVDGEWKHDPKMKNIENDIGTKNNMISVRQSDFEVFQALAKDSEDAANNGQKEYSQEIPQSKPWEKVSGPPVLPPHLLQVILNKDTPLSVCTFLYSILFLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN01</accession>
    <name>T1GN01_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Seven cysteines N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN01"/>
    <dbReference type="STRING" id="36166.T1GN01"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004940-RA">
        <property type="protein sequence ID" value="MESCA004940-PA"/>
        <property type="gene ID" value="MESCA004940"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1059942_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013980">
        <property type="entry name" value="MANSC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011106">
        <property type="entry name" value="MANSC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46876">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46876:SF1">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07502">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00765">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Seven cysteines N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004940-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="263" mass="29393" checksum="552BBE965D61BAF6" modified="2013-10-16" version="1">QFDVHHNTIIRTEESLEIENCDAFVFEEKKDGYCYLFQCGPPENMHCKFTKHANYTSSLLTPVRPTIPSTTHKPLISTYISQQEIQLSNLKKAPKEDKKPMTSTTQAPEIPAKFELPRLLPTSKSIPPNSSSCGRFQFACHSGECIAVYNACDGIPQCEDGSDEGPECSGANAKSASNIVATSVDSPTKIVQQPIQTIQNQYKINNREDPLSSPPVWSTGNELDHTRTSYYRAIVPVHRKGTSFLFHTFIMAKIYYGQVTIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN41</accession>
    <name>T1GN41_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004980-RA">
        <property type="protein sequence ID" value="MESCA004980-PA"/>
        <property type="gene ID" value="MESCA004980"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870189_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004980-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7562" checksum="E6CA2CB95E2A2036" modified="2013-10-16" version="1">MVHCSILLIPIWSVIRLPKIDEEDLYECEKLVGVRFSRYKTELIGANNGDYNKLKWNSSFFVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GND8</accession>
    <name>T1GND8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175730">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GND8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005085-RA">
        <property type="protein sequence ID" value="MESCA005085-PA"/>
        <property type="gene ID" value="MESCA005085"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286127_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007931">
        <property type="entry name" value="TsetseEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05267">
        <property type="entry name" value="DUF725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577502" description="Protein TsetseEP domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Protein TsetseEP" evidence="3">
        <location>
            <begin position="30"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005085-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="23907" checksum="4A51DF3BBDBC5898" modified="2013-10-16" version="1">MKSLPVFVILILFSVVSAQFPFLFWKKMDTVVCYNFYGECLTKYSNRAARESEICESTRNETLKGILVDFNGVLGELLRSIEIVKNNIDCGSKNETEQKLCLNNIARNNINSLKDIENLSINAYNRRSHNEIIANSVCNGCLSDVNIKASEAIQEIDSILRSCYLTGKYINPKHEWFYGDLATSTTKSSTILSTTSSPATNTIGSSSTTTTVEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNG2</accession>
    <name>T1GNG2_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02086607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02086608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02086611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02086612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005113-RA">
        <property type="protein sequence ID" value="MESCA005113-PA"/>
        <property type="gene ID" value="MESCA005113"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592500_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005113-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8766" checksum="C0C4BC82DDB41BE0" modified="2013-10-16" version="1">MDERVCTIDEIVLPPPPPPPAKSPPNGDTNVVVCLPPPASFSNNPYPNNHQSSQHPSNSMHPLNFQNQNQQKSSNVLKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNM8</accession>
    <name>T1GNM8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNM8"/>
    <dbReference type="STRING" id="36166.T1GNM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005186-RA">
        <property type="protein sequence ID" value="MESCA005186-PA"/>
        <property type="gene ID" value="MESCA005186"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874316_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005186-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7619" checksum="D44F726E0A859BB2" modified="2013-10-16" version="1">NRNRPQFGDDEEYEHAKKNLNRYGKIRTLSKSEWEATSLYLVCAFQKCKNTWDANGKPVYEFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP50</accession>
    <name>T1GP50_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="CAQQ02392280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GP50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005367-RA">
        <property type="protein sequence ID" value="MESCA005367-PA"/>
        <property type="gene ID" value="MESCA005367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_0_0_1"/>
    <dbReference type="OMA" id="KPCMGIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.140:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="16">
        <location>
            <begin position="224"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1420"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1523"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1594"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1430"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1452"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1523"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1540"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1550"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1612"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1634"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1650"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1671"/>
            <end position="1690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1691"/>
            <end position="1706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1746" mass="192929" checksum="6D8A8935FADB93F9" modified="2013-10-16" version="1">MATNDSKAPLRTVKRVQFGILSPDEIRRMSVTEGGVQFPETMEGGRPKLGGLMDPRQGVIDRSSRCQTCAGNMTECPGHFGHIDLSKPVFHVGFVTKTIKILRCVCFYCSKMLVSPNNPKIKEIVIKSKGQPRKRWRRHGCWATKNGMDPNDPNKKQGHGGCGHYQPSIRRTGLELSAEWKHVNEDSQEKKIVLTAERCWEILKHITDEECFILGMDPKYARPDWMIATCLPVPPLAVRPAVVMFGAAKNQDDLTHKLADIIKANNELKKNEASGAASHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNMTFPELVTPFNIDRMQELVRRGNSQYPGAKYIIRDNGERIDLRFHPKPSDLHLQCGYKVERHLRDGDLVGQSIALVHFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEIENIHITPRQIITPQANQPVMGIVQDTLTAVRKMTKRDVFITREQMMNLLMFLPTWDGKMPQPSILKPMPLWTGKQLFSLIIPGNNVNMIRTHSTHPDDEDDGPYKWISPGDTKVMVEHGEHIMGILCKKTLGTSAGSLLHIVFLELGHEIAGRFYGNIQTTINNWLLLEGHSIGIGDTIADPQTYSEIQDSIKKAKDDVIDVIQKAHNMELEPSPGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVGQQNVEGKRIPFGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGENVEFQNLPTIKLSNKSFEKRFKFDWSNERYMRKIFNEDVIKELSESGYALQELESEWEQLTKDREALRSIFPSGDSKVVLPCNLQRMIWNVQKIFHINKRVPTDLSPLKVIQGVRDLLNRCVIVSGTDRISKQANENATLLFQCLIREIESRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDPDPQRTVIAEDQEFVNVYYEMPDFDATRISPWLLRIELDRKRMTDKKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKFQDGAEEDMDKMEDDMFLRCIEANMLSDMTLQGIEAIGKVYMHLPQTDSKKRIIITETGEFKAIGEWLLETDGTSMMKVLSERDVDPKVCGKGNERCFVFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVLLDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCKNGIDFPTTMGALGLGNAGVFFGVGSTPARTPWAATGATPSYFSSPGQMAGMTPGPSFSPSAQSDISGLSPVWSPAHPGSSPTSPGMPMSPMSIPMSPSASPAYSPSSPNYAASSPGAASPNYSPNSPNYSPTSPYYYPGGGSTSPFIQSASYSPNSYSGNSNFSLKSPNYTGGASNSYSPSSSYAYSSPHYSPTSPGYSPTSPMYSPAGSAYSPSSLVILGSSPPTYSPQSPAYDGSSPKYSPTSPSSPTYSQTSPSYNASPQYTPGSPQYSPASPIYSPTSPLYSPTSPVHSPGHQYSPSGSQFSSPRYSVNNMSYSPSSSSYSPASPAYTPESNNYSPTCPRYSPTELGYSPTNAVYSPSSPPFEDKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPF1</accession>
    <name>T1GPF1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(+) ADP-ribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02151536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPF1"/>
    <dbReference type="STRING" id="36166.T1GPF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005479-RA">
        <property type="protein sequence ID" value="MESCA005479-PA"/>
        <property type="gene ID" value="MESCA005479"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_437003_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17747">
        <property type="entry name" value="BRCT_PARP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08001">
        <property type="entry name" value="WGR_PARP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038650">
        <property type="entry name" value="PADR1_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012982">
        <property type="entry name" value="PARP1-like_PADR1_Zn_ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08063">
        <property type="entry name" value="Zn_ribbon_PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01335">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52007">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="109"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="WGR" evidence="8">
        <location>
            <begin position="254"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="340"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005479-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="626" mass="69686" checksum="18F92418329788D2" modified="2013-10-16" version="1">MTRVQMQELLEENGQEPVTVCKEGQFIFNNSGYLCTGSISEWAKCANLVKEPPRKRCKVPDSFKESFSFLKKVKKSPENRFIRYIAPSASTLAKRVKKEDTLDGPKVKRERPPLYNMTFCLVGIKDSDGEIKKKISSLGGVLSSSLNEKTTVIISNEKEVEKMGSRMEKAHEMGIHVVPLEYLDAVKEDPSGAISFINSKTLCDWGTDPAARIPQDELKSGKSKSIYTKSVPKSMKVTLKDGLAVDPDSGLEDIAKVYVKGKDKYTVTLGLTDIQRNKNSYYKLQVLVGDTKNKFWLFRSWGRIGTTIGGNKTESFNTLTDAINEFERLYLEKSGNEFSDHSGVYSPSPANTASSSSPTSTHHSSISSPQTPTGGGGGSGTNERTSGHKNSLKGTKLARRARSFKDDLFERISQIRTPTNTIGRAHSPHSPRNKHSSSASQMKFFVTSEDKNKTSYQLETHEKHIKNALRHLCDVISKKKFEVLPGNGTILLDLITSLHAVMQKVIMNENSTTFISATTKMYYSLGKLIKLCDEFLLNEDENDCSSLNTENVTGVAEEVEVAVQQLVQLATEKAKEKEKEKDGQKQMNNNTLKPTVVDIAAQRTSLPDIPLTPKERDILRNLHQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ03</accession>
    <name>T1GQ03_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005700-RA">
        <property type="protein sequence ID" value="MESCA005700-PA"/>
        <property type="gene ID" value="MESCA005700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888344_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005700-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7178" checksum="304C286943B2B658" modified="2013-10-16" version="1">MGQHLDVYHGIGFLANSIISGHIRVGKMMKLMNLRNNYECRFCEDLYAVECLKHLAGEFCSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ08</accession>
    <name>T1GQ08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02025759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ08"/>
    <dbReference type="STRING" id="36166.T1GQ08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005705-RA">
        <property type="protein sequence ID" value="MESCA005705-PA"/>
        <property type="gene ID" value="MESCA005705"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011554_1_0_1"/>
    <dbReference type="OMA" id="HIKVWIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000075">
        <property type="entry name" value="Crooked neck pre-mRNA-splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000327">
        <property type="entry name" value="Pre-mRNA-splicing factor CLF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02184">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="8">
        <location>
            <begin position="306"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005705-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="610" mass="74160" checksum="B7EB54D2C3D356F0" modified="2013-10-16" version="1">MERPQKMPKVAKVKNKAPAEIQITAEQLLREAKERDLEILPPPPKQKISDAAELADYQQRKRKFFEDGLRKNRMVVSNWIKYAQWEESQKEIQRARSIWERALDNDHRNLTIWLKYAEMEMKNKQVNHARNLWDRAVTIMPRVNQFWYKYTYMEEMLENVAGARQVFERWMEWQPEEQAWQTYINFELKYKEIDRARSIYERFVYVHPDVKHWIKYARFEENHGYINGAPFAKFEEGQKEHERARVIYKFALDNLSKERTQQIYKAYTIHEKKFGDRAGIESVIVSKRKHQYEQEILDNPLNYDAWFDYIRLIEDEGIVDNIRETYERAIANVPPSKDKNLWRRYIYLWINYAFYEEIEGEDLERTRQIYKTCLELIPHKTFTFSKIWLFYAQFEIRCKNLQQARKILGMGLGMCPRDKLFRGYIELEIQLREFDRCRILYEKFLEFGPENCITWMKFAELESLLGDDERARGIYELAIQQQRLDMPELLWKGYIDFEVNQGEFENARQLYERLLEKTQHVKLRQAGDKESRVLILEAWRDFEKEHGDKENYEKVLQKIPKRVKRRQKIINDGVEEGWEEVFDYIFPEDELSRPNLKLLAAAKMWKKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR19</accession>
    <name>T1GR19_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006090-RA">
        <property type="protein sequence ID" value="MESCA006090-PA"/>
        <property type="gene ID" value="MESCA006090"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087573_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006090-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13714" checksum="3C9BC17D38717F5A" modified="2013-10-16" version="1">MDLQSFYQEYISPHFVRAFIVCISFKMLQFQKTLVILLAMLMGFAFYYGIQLFDFIFEDTNLFNIDLFKKSIAVWTGTILGMLSYSLTMGRSLFQTYIFALIGSFISTLYIEIKQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR93</accession>
    <name>T1GR93_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393346">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR93"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006171-RA">
        <property type="protein sequence ID" value="MESCA006171-PA"/>
        <property type="gene ID" value="MESCA006171"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906647_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006171-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6914" checksum="9445B94F58541B95" modified="2013-10-16" version="1">MEVRSSTLEGMKTPETSPVTKGRSKKELYRIVSCERSVFCPFGVRETAGNLLLNDMGFEDQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRB2</accession>
    <name>T1GRB2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02126260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126264">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRB2"/>
    <dbReference type="STRING" id="36166.T1GRB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006192-RA">
        <property type="protein sequence ID" value="MESCA006192-PA"/>
        <property type="gene ID" value="MESCA006192"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2336002_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0472">Membrane</keyword>
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    <feature type="domain" description="MSP" evidence="6">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006192-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11061" checksum="07A11CC57D5F39C6" modified="2013-10-16" version="1">MKVKNLEKLYDFFDTDEMLRITPCDLIVFSGDSEIHGSVEICCIGRESITYKIKTTSPEKFLVRPSSGILQPGTSAKINVTLQKNFDLVNVNKDKFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRY2</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDAH family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA006429"/>
    </dbReference>
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        <property type="term" value="F:amino acid binding"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:positive regulation of nitric oxide biosynthetic process"/>
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    </dbReference>
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        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737:SF9">
        <property type="entry name" value="DIMETHYLARGININASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737">
        <property type="entry name" value="DIMETHYLARGININE DIMETHYLAMINOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006429-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23127" checksum="40B7CECAEC3E4891" modified="2013-10-16" version="1">MKFILSIPSMFFSSSKQLMSFFNNFLRLSDKPGHANQKCSGNDALKIVLKKELDIPTVDVQDETAYLDGGDILFTGREFFVGLTERTNEGGAVAIAEAFPEYPCVPIKVSGDKCLKYYISMAGPSLLAVSGSEYSQDILKRIERVASFSYQTLTLDEEFAANMLYINGHLIHRSPLEIPESHRIIKSKIDTPSRNVDISEISRFSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRY6</accession>
    <name>T1GRY6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA006433-RA">
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        <property type="gene ID" value="MESCA006433"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
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        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
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        <location>
            <begin position="109"/>
            <end position="144"/>
        </location>
    </feature>
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            <begin position="152"/>
            <end position="229"/>
        </location>
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        <location>
            <begin position="256"/>
            <end position="372"/>
        </location>
    </feature>
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        <location>
            <begin position="400"/>
            <end position="476"/>
        </location>
    </feature>
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        <location>
            <begin position="539"/>
            <end position="598"/>
        </location>
    </feature>
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            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="642" mass="73720" checksum="68E063F3A0911A97" modified="2013-10-16" version="1">MVKKSESNSSAHEMAKNIAEKLSNLKTKAKSRQSLNKSINDLHQTADVPDISLITSTPPPIRRESAGTPGSQKMELLKQQMEVNRQKLAERKNSKKEIEEMVSQLRANFSSAQQSLEHSSELGRSMGDLSSLSYTPGKSKVKSTTDLSFTPLNLDKERIKFLENRIRHLEKQQEKVVSSPDEIVALQKRIQELEESLSEKEDLENDNQVQTLQARIKELEETLSNQEDNQIKDLKFQLEERLKEIEHLKTGDNIEIEDLRYQLDEKIHQIESLKHQSGSQEEIDSLRALLDQKEHDIQELKKLKEKIQVQAVTTIFDDEMEQQLRDEMEDLKQKLEKSADENESLVKENKELKEVNDMLETLRCDLKVKNSELEEKIVQMEESQRFEIVTKEEVETTQIGDEFMSKIRELESKIQLLEQENEELKGSQNLSQISDENAVDAIKEMESTLDQQRQKIQSLEDQLAEKVIECNVLNANFSVLEEKLKSTATPKTLFPSADEASDAEIVKLKTQLDEVNKSMIKMKVKSKQQQKQIDNFKKMSSANDEIIKLNEEIEKLQGKIKELESSQIESIDIDEVEINALKEKVSQLTAENAELHHKLSDKASSSEKAEGEEKCLETCERVSFLEEQLQKTIDEKDGISSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSE1</accession>
    <name>T1GSE1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSE1"/>
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        <property type="gene ID" value="MESCA006596"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
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        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
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        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="135"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="303"/>
        </location>
    </feature>
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            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006596-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="333" mass="40605" checksum="27E8CA760673AC48" modified="2013-10-16" version="1">MYLPEVGGGHDHNDHHHHECSCPPGPPGPPGQPGIPGIPGRPGINNNNNNNIRRRYYEDSGYTKIYEDRYRPSHHYRPPPRDYDRRPIPPPRRDHDIYPETPVVGYDERDKYREDHKRPMPPLREYEDDKEEMSEDDKRPIPPPRKDYDDRMPEDNRRPYDDRDRYPEDDRRPYDERDRYPEDDSRPYDDRDRYPEDDRRPYDDRDRYHEDDRRPYDDRDRYPPHREYDDRDRIHEDDRRKPHREYDRDRIPEDDRRRPLRDYDDRNRITENHRKPPRREYEHNESSSANSTSSSNSSSSSSSHVLYDDDIRIPEDAPDDDFKNRQNTGRGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT37</accession>
    <name>T1GT37_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006857-RA">
        <property type="protein sequence ID" value="MESCA006857-PA"/>
        <property type="gene ID" value="MESCA006857"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592524_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006857-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9351" checksum="6EFD7BCC161CD010" modified="2013-10-16" version="1">MRDRETHPKEDKRNKPYLYEANEFVGGLSVQLRNIPFLRSIQLEGSNLFQSFHILYCFQHSIAFPLKYIAVTSSSISCIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTN5</accession>
    <name>T1GTN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WW domain-binding protein 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GTN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007073-RA">
        <property type="protein sequence ID" value="MESCA007073-PA"/>
        <property type="gene ID" value="MESCA007073"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1075937_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361:SF1">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007073-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29256" checksum="435A2A0B6B9319F9" modified="2013-10-16" version="1">MVRTTALKNKDPSQILDEMEKIDEMEYNVMQDSPLNEKVLKDKRKKLKETFDRVMKVYHQEEPEHWAELKRREVEYEKKRLKKEQYYESVKHAQSVQIDEIPLPQMSTMPAPGIPQQRVPPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMESDRSEESDEIDSDEETELPPIPGMNTAVPSIAPALSALPTNLPPHPTAAAIAPPIVFRPPPLRPGLPNFGIRMPPGPPVGPRPGSVAPRMNIRMPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTU5</accession>
    <name>T1GTU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA ligase (ATP)</fullName>
            <ecNumber evidence="2">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02060439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTU5"/>
    <dbReference type="STRING" id="36166.T1GTU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007139-RA">
        <property type="protein sequence ID" value="MESCA007139-PA"/>
        <property type="gene ID" value="MESCA007139"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004844_2_0_1"/>
    <dbReference type="OMA" id="IMLQHRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005958">
        <property type="term" value="C:DNA-dependent protein kinase-DNA ligase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07903">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044125">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029710">
        <property type="entry name" value="LIG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997:SF1">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="13">
        <location>
            <begin position="308"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007139-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="707" mass="82142" checksum="A57A8F7489FAD976" modified="2013-10-16" version="1">MHETNFILICEILEKIQESKSIQRKENILKEFILKFSGEKYYTYPAIRLLIPILDSDRPKSGLAVNSIAKIYIKSLQLSNKSIDAQKIFLITKPRISIKQSECTLEEINKFLDIVCNSSRKDVEFEFKKFIEKLSSLEQKWILNIILKKLDLGLSAEKILNTIHPNASEFYNRKTDLKFLCDQFNKGKLNFGVCGTSLFNFVKPMLCQRVDSKKINEILSKNDEFWAEEKMDGERFQLHYQKLNSKFILFSRKGIDYSQKFFKTIEILSKLFKGNIENCILDGEMMVWNSQSKTFKVKGEFIDVKNLVSSNINNPCFVCFDILYINNKSLIDLSYSDRLQLVKEHFKTSLGQFHILNQSKIRNVSEFTSYFNKSLENNCEGIVLKQVSSTYKPGQRVNDWIKIKPDYIDGLITDLDVLIMGVNTRSNYIESFYVGVLDEKGTLHCIGKVSSGLSESKKKYLTNKLKPFYMVKKPSNAEFGNEKPYMYLNIKKSIILQIRASELVLSDSFKTPYTLRFPRIDSIRDDKEWDSCLKLSDFEHLYQSLSSVAKIHKRKTCDEDFSGKSFKVNKEKLMNPYQTVISHPTEVLSEKFADLNFCILSPTKNHTVSEAKEIPLKCICIAGELTPKVKSLLNLRKYNIICFDWLLRQYNNPKTTIKISPKDLICSTSELTEQMKEYYDEFCDSFDDFISENELKEILININSNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU06</accession>
    <name>T1GU06_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU06"/>
    <dbReference type="STRING" id="36166.T1GU06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007205-RA">
        <property type="protein sequence ID" value="MESCA007205-PA"/>
        <property type="gene ID" value="MESCA007205"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152066_0_0_1"/>
    <dbReference type="OMA" id="NDCISCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF30">
        <property type="entry name" value="SUGAR BABY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="8"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12303" checksum="AB3EE76EFC026A7A" modified="2013-10-16" version="1">MKPKINKELLPMKGHYFLFSAGTAPVVPFMPTLARQMGFSSVVVGTIYSILPIIGLLTKPIFGFVSDKFQSHKLLFILAQGLTAVAFFCIMFIPNIPTTVDFHCHGGESVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUA7</accession>
    <name>T1GUA7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007317-RA">
        <property type="protein sequence ID" value="MESCA007317-PA"/>
        <property type="gene ID" value="MESCA007317"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533980_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9812" checksum="A2CE45126A3522A5" modified="2013-10-16" version="1">FFGYHSISILPALSKKHVNTTTAVLNITDDIRFQIDNNLYCFLFLLDLSRAFDMIDHNILLYKLRKKLKFLNVACKLLKSFLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUD8</accession>
    <name>T1GUD8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pcf11 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GUD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007348-RA">
        <property type="protein sequence ID" value="MESCA007348-PA"/>
        <property type="gene ID" value="MESCA007348"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_250973_0_0_1"/>
    <dbReference type="OMA" id="CETEMRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pcf11 C-terminal" evidence="3">
        <location>
            <begin position="1009"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="860"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1295"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1399"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="864"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1301"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1401"/>
            <end position="1410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1457" mass="163690" checksum="41C6A4D3E1CDDA0D" modified="2013-10-16" version="1">MGPPIDPSNAEVAKTISEKQRELLELQKRKLELELEETKQKLLQQEMVNHNAGVMLPGARMAGPGIRGHFPSQYRGPVQRPPNSGQTGHASLLRKERSKSIDERSKRTESRSKSVDERQKNRSSSSSSSSRREREKSDRSDRSGSESGSKSSPTRVPRSNSRSKSSHRNREEKTKSPEKKKSSSSSSSKSKSSSSRSDGSGDEKGSPTKFKEVKGSSIKNRNYIRKNMGSEGSFSPKEDVDLRSLEASPEKRLKLSTIPEDEQNGVVKDLVNNNNSVNIAEEDLKKRSLTDVPGENQPSPKRVKNDKLVDDLFGSSDVDLRAMPAIFDPEDSPLEKDKKPKSFDDVRAKLGKAVKPRSKDPLKKITQIDINEDSQEGKEANMMTILAQAQEMVENEAIGKDQYNNLIQTVIHLNETNKLKEAKRRESLNINENPKKSKEAVTNDSDERTELKSILSSLDANVTDPKPNRVTEKVVAPTITKRRPSKWSPINPAWEEQNQPPVRQQWIPNQRPPPIQMPPQQPHPMQQIPHMPPQQQPQHFAWEPPMLFNNQNHNQNPVNQVPPMQVIESLPAPCNSVNNPQEDVIRSITIDGSCRETRMYGKTAVVFMEWDKPIELGFKSGMRRIWIDDMEPVCLSFNNDYVPVNIGGATYPVKFGVPSRELYIGDHWYECFFGGRQIQIPIDGRMHNLRVEGPPPQVNLGNIRQDLVVAKINMIIDAKFVIPIFLDSKPQVFVVDDEKHVIQFADNLKTALIDGDPINVSYGDLPKHMKLGERRNVYVKDMIYINDSPREEPKEEDHLMKEPTVDLTEEAPVSVAPEVAAPVTVAPPPVVPSVAPPPVVNVDELLQKLISSGIINNQLTIPKPAEKPKEKEEEPVKEPVKPPIQYNPINFSKPETIKTRQQGIVDRLMFTGMQCSSCGLRYPPEQTIKYSQHLDWHFRQNRRERDLVRKAHSRKWYYDLNDWKQYEEIIDLEENTKNYFEAQEDGNSGDFVDDSSNQRSNLSPPPSCPAGPEDVDRACDVCHERFEQYYNEDLEEWHLKHAIRMDDRFYHPLCYEDYKASLTMDESAIANISADDNAVDVRLKRRSRLLQKVNILWKYLKHSYYLQTNIDYIYDKLETIDDDDDDDDVIVVPIEEPSITEIPDDDDNDEYVPQVVPKDTSKTEETIDDEESLLHDSMETDVVIQEKTIPFLDLDNFDESAMSPVVYQPVKIKEEPKDEDEVEEDDGFEDVGTVVEGAVDEITIESDSIDFANANTDSQPATGEAMPLVDTAMTMDGNTVAPQSITSVNKIRINITKPKSTTDPAAATSSTSGGSSSGTTTGNIDESNSNCNNSNIPVLCSSGVSNIQTIVSLNSNSSNNNICSLSNNINIINNKLNNNSNEPDADTCNSGIDGGVEFLSNHHQSSSSADTAAAPEEEDSSLLFPPPVFKLEMQNVDFKKMPKTQVGLETSGLCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUK0</accession>
    <name>T1GUK0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 242</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Scaffold protein that participates in the c-ring assembly of mitochondrial ATP synthase (F(1)F(0) ATP synthase or complex V) by facilitating the membrane insertion and oligomer formation of the subunit c/ATP5MC3. Participates in the incorporation of the c-ring into vestigial complexes. Additionally influences the incorporation of subunits MT-ATP6, MT-ATP8, ATP5MJ, and ATP5MK in the ATP synthase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM242 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007414-RA">
        <property type="protein sequence ID" value="MESCA007414-PA"/>
        <property type="gene ID" value="MESCA007414"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2040737_0_0_1"/>
    <dbReference type="OMA" id="KSPAWFN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009792">
        <property type="entry name" value="TMEM242"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13141">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13141:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07096">
        <property type="entry name" value="DUF1358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007414-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="13276" checksum="370F4593BA32AADF" modified="2013-10-16" version="1">MTTKTEIDKAEEDRKHRIRGAAFLGLVGGVSALFGFSKTLTKAKRQDSKLIEKGGREALILMDEGSALALRALGYGTAYAVLGTGLFCFGVWKLSGAKDVSILIYLDDNDMNQRFIPFERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV51</accession>
    <name>T1GV51_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP synthase F1 subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase inhibitor family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02108457">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV51"/>
    <dbReference type="STRING" id="36166.T1GV51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007633-RA">
        <property type="protein sequence ID" value="MESCA007633-PA"/>
        <property type="gene ID" value="MESCA007633"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147479_1_2_1"/>
    <dbReference type="OMA" id="WIAPSAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000007">
        <property type="entry name" value="ATPase inhibitor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007648">
        <property type="entry name" value="ATPase_inhibitor_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48417">
        <property type="entry name" value="ATP SYNTHASE F1 SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48417:SF1">
        <property type="entry name" value="ATP SYNTHASE F1 SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04568">
        <property type="entry name" value="IATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64602">
        <property type="entry name" value="F1 ATPase inhibitor, IF1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="11003" checksum="717C8EA9095EC4FC" modified="2013-10-16" version="1">MFQLQRTSRFLPTVYRCCQMSGAGTIRQAGGSFGKMEAAHEEEYFRRKQFEALQKLHSDQIHQIEFHKHQIKEHQEAIKRHQEFITNLEKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVG6</accession>
    <name>T1GVG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007769-RA">
        <property type="protein sequence ID" value="MESCA007769-PA"/>
        <property type="gene ID" value="MESCA007769"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415790_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007769-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10911" checksum="8316915BC602E4A5" modified="2013-10-16" version="1">MVIITSNNPFMKKFSRDNRMCSNDLCPAPQKLKDFSSGYTYCCNLDEFVYNMKRKYNTEFNGIIFPQKDIKFFEQESKKKTDLGDNLQRIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVQ8</accession>
    <name>T1GVQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVQ8"/>
    <dbReference type="STRING" id="36166.T1GVQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007874-RA">
        <property type="protein sequence ID" value="MESCA007874-PA"/>
        <property type="gene ID" value="MESCA007874"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1273517_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000417">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF28">
        <property type="entry name" value="UROMODULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007874-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24686" checksum="62EF162963316FD0" modified="2013-10-16" version="1">MKRRLQGKHLPLPPTMNGTQWGQATMGKNILKGCISNNMCLNVVCPDPFECNNIWMGYECSCGDGRIKSPDGRRCLDRNECLEDKPCMNGGTCINLEPRRRYRCICPDGFWGENCEILQEEQTNSKIYYSSLAAILVITVLLIVSDGSVNWIPDENDFCGYIYIYRVWMFSDAILMNFNSDASLWEISFLRNRTVGNAGNGNNEKSIELERLQLQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWF7</accession>
    <name>T1GWF7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWF7"/>
    <dbReference type="STRING" id="36166.T1GWF7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008132-RA">
        <property type="protein sequence ID" value="MESCA008132-PA"/>
        <property type="gene ID" value="MESCA008132"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023062_5_1_1"/>
    <dbReference type="OMA" id="NSHQCNL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08839">
        <property type="entry name" value="ArfGap_SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044732">
        <property type="entry name" value="ArfGAP_SMAP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577705" description="Arf-GAP domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008132-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="377" mass="40708" checksum="9D7FA38B126A75AB" modified="2013-10-16" version="1">PRWASWNLGVFLCIRCAGIHRNLGVHISRVKSVNLDTWTPEQVLSLQQMGNSRARAVYEAQLPDGFRRPQTDNALESFIRAKYEHKKYIAREWVPPSPPKVDWNKEIDEELEKQKKKKKPTGGIGLVTGLNSSLEKKSISNSSISNVKTSPLPAPLPKPKVSAASNSPKNTTTSGSMDLLGLTSPVSQPSQQPIASDSFTGFLSAPTNGASNGQTQESNKSSQASNGGAGGDDNLSLAKEEEDFFKQTTSLPNDGGGIGKMSKDSILALYGTAPSGMQFQSNPLSSTFPQQSGNTGWGQPQAHGQFMQPPNSATIYNAPVMTAPAAFSHPQMMMGMQQSMEDLLNSHQCNLSNSNNLLCPPIRLVGRKLWAVDLRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX16</accession>
    <name>T1GX16_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX16"/>
    <dbReference type="STRING" id="36166.T1GX16"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008354-RA">
        <property type="protein sequence ID" value="MESCA008354-PA"/>
        <property type="gene ID" value="MESCA008354"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1490664_0_0_1"/>
    <dbReference type="OMA" id="SIHEIGM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577722" description="Partial AB-hydrolase lipase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="2">
        <location>
            <begin position="29"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008354-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20955" checksum="66A47FAB66915B2C" modified="2013-10-16" version="1">MQASTTHILIVLFLRTISIDGTTYNRAEYKIIEDGFPLEIHNITTDDGYILKAHRIPNPGRPVMLLNHGFVASSDAFLWLGKNHALPYQAFEANFDVWMLNCRGNWYSSQHKKISSLHPKFWKFSIHEIGMYDLPAAFDYILKTTNRTKLHYVGHSQGTAVFLIFQSERQSIINLWPQLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYD1</accession>
    <name>T1GYD1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GYD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008856-RA">
        <property type="protein sequence ID" value="MESCA008856-PA"/>
        <property type="gene ID" value="MESCA008856"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1043121_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="2"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008856-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="267" mass="30795" checksum="D9A5A8A351994A6B" modified="2013-10-16" version="1">MEQKQLILDTHNELRNIIATGKPQLVNIAGELFPKASNMHKLIWSDELEWQADLNAATCSANHDCPSTENYMSASQNLDTILNWFYEFIYTPLEAIDSYSSKTVKNTDKLTEIEAERVKNLTGSSFFFNLNSHFTTVVKAKTSKIGCSFYSCGQVRDLPYSWYFVCXSKIGCSFYSCGQVRDLPYSWYFVCNYEFMNMFGEATYKHNVPENCPRSKKYCGLCSEENEEELSCLESDLDFQSFKKSSAKLNYVSMYLMLIVVLFRFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYD5</accession>
    <name>T1GYD5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYD5"/>
    <dbReference type="STRING" id="36166.T1GYD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008860-RA">
        <property type="protein sequence ID" value="MESCA008860-PA"/>
        <property type="gene ID" value="MESCA008860"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199970_0_0_1"/>
    <dbReference type="OMA" id="TIPKQLW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008860-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12163" checksum="C7CE2F7879FAB18D" modified="2013-10-16" version="1">MQIHLQEPPGDILLFLTGQAEIDTACEVLYERMKNVGPDVPVLFILPIYSSLPSEMQTRIFDPAQAGSRKCIVATNIAETSLTIDEIFYILQDLSNKKITIPKQLWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYN4</accession>
    <name>T1GYN4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYN4"/>
    <dbReference type="STRING" id="36166.T1GYN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008970-RA">
        <property type="protein sequence ID" value="MESCA008970-PA"/>
        <property type="gene ID" value="MESCA008970"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2055582_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008970-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13384" checksum="2B1FBC7E756194DA" modified="2013-10-16" version="1">MQHTHLTQPILLNNSVNNNCQQLQVNSSSPGSTTSDSDNHLNNIHSNIANNQGSNNNYNEGNMNMKSLQGHEHMDTLQQKLGKQIIAKPLPSRPTPFIAHNINHPHLHSLLAHCRNPYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYN9</accession>
    <name>T1GYN9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02139675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYN9"/>
    <dbReference type="STRING" id="36166.T1GYN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008975-RA">
        <property type="protein sequence ID" value="MESCA008975-PA"/>
        <property type="gene ID" value="MESCA008975"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_1"/>
    <dbReference type="OMA" id="DGHIMES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097254">
        <property type="term" value="P:renal tubular secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017085">
        <property type="term" value="P:response to insecticide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002695">
        <property type="entry name" value="ABC multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000009">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000479">
        <property type="entry name" value="Multidrug resistance protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="410"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="184"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="503"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008975-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="746" mass="82576" checksum="E4DC86B314B48340" modified="2013-10-16" version="1">MANAHDFISKLPKGYETRLGERGSQISGGQKQRIAIARALLKDPKILLLDEATSALDPTSERKVQDALDAASINRTTLVVSHRLSTITNADIIVYVKDGQMAEQGTHNELMAKKGLYYNLSMINRKQENDLEGKQRKPSYKDEDDDDEMIDDIFSVGGEKIKEKYKISLYSLMKLNAPEWKLILVGCIAACLHGATFPIWAVLFGEFYGVFASLDNDYVQQQSDKYSYYFVAVGIVSGLGIFMQTYLFTIAGVKMTSRLRQMTFKAILSQEMGWFDLKKNSVGALCSRLAGDCSNVQGATGQRIGVLVQAIATLVVGAIISFVYSWNLTLVTLITVPLLLGSVIIESKYMEKSTMKERNAIEVASTIAVEAISNLPTVTSLGQEPYVLKRYCDQIEIATRACEKSVRFRGLVFGFGQSMPFIAYGISLFYGGYLVADGLEYKNIIKSIDIWLLDLGQALAYAPNVNSAIISAGRLLDLFKRVPKMENCAPSPFNAVEKIEGDIKYENIEFKYPTRQETPILQGLNLEIKKGQTVALVGPSGCGKSTCIQLLLRYYDPDFGSLNLTGTPSTMFPVDVLRSQLGLVSQEPVLFDRTIAENIAYGNNFRDDIPMAEIIESAKKSNIHEFIKSLPQGYETSLGNRGAQLSGGQKQRIAIARALVRNPKILILDEATSALDNESEKIVQEALDAASTERTCITIAHRLTTIQNADIIYVLKNGEVHEKGTHTELIQLGGIYKQLYKMQQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYV1</accession>
    <name>T1GYV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYV1"/>
    <dbReference type="STRING" id="36166.T1GYV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009038-RA">
        <property type="protein sequence ID" value="MESCA009038-PA"/>
        <property type="gene ID" value="MESCA009038"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_759274_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009038-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="365" mass="42103" checksum="51BE661197F72AE5" modified="2013-10-16" version="1">MLNADKCLISLEDARSYIADLYLGYVKKPTFLVKRSFVHIKIERSELISNDVLLTLDIEGTLSTWDISNEKKPAIKRTSSTKLVNSLNAMSSIKNKTSIQDLNDNFSDKVKSFHINKDGRLLIGDSKGEITMMKWNERTKRFRKLEVQKIKTKIINMKYIHDLGDHHCIVVDHDGKIFLLAITTGLRDRTFLQIYAITIDFKWSYEQSEYEHFPRNIYLKSGNQDFSASYSSYHNDCEMFAIDSENKIHNFNKDEKNYKRIPVQVTSIASGLRTFYGCVNGDIYNFKTDTKLQLNQIVSPISYMKVIGNILVASADSGYTILYEIHENITYIINTGGKLLDSWFLNNNVVVLFNTNEICKEKWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZB1</accession>
    <name>T1GZB1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02094780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZB1"/>
    <dbReference type="STRING" id="36166.T1GZB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009209-RA">
        <property type="protein sequence ID" value="MESCA009209-PA"/>
        <property type="gene ID" value="MESCA009209"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043552_3_2_1"/>
    <dbReference type="OMA" id="ENICMAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000014">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009209-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23676" checksum="F479EB5D60858E32" modified="2013-10-16" version="1">MENKQQIQHPIKPSQPYENNKPSWINNDDSDDIHVLFMVLKKGDTPGHGLQQSLHFVGRCATLEQWWSLYCHLVRPSALHPYRELSLFKHGMKPMWEDPANSKGGQWVVRLKKNRIDRAWENICMAMLGEQFMVGPEICGVVLTTKYPEDSLSVWNRTATDQTCTARVRDTIRRILNLPLNTPMEYKTHCDSLKYLAMAKGPLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H004</accession>
    <name>T1H004_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-1,4-glucuronyltransferase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">I-beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Poly-N-acetyllactosamine extension enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-O-[beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP-alpha-D-glucuronate = 3-O-[beta-D-GlcA-(1-&gt;3)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46860"/>
            <dbReference type="Rhea" id="RHEA-COMP:15023"/>
            <dbReference type="Rhea" id="RHEA-COMP:17482"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:142405"/>
            <dbReference type="ChEBI" id="CHEBI:177336"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 49 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H004"/>
    <dbReference type="STRING" id="36166.T1H004"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009466-RA">
        <property type="protein sequence ID" value="MESCA009466-PA"/>
        <property type="gene ID" value="MESCA009466"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_635505_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043189">
        <property type="entry name" value="B4GAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420:SF1">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009466-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="432" mass="50202" checksum="A7F16A6C1296398C" modified="2013-10-16" version="1">WVKDTNESPETHVSHNVTNFEFLYEPFYVATDLVPTHDERFMGYGFTRNSQVYEMYVAGYQFQVLSPIFTIHWGLQQKKTRPAWREQQNNMNRKQFEMFKKEIILKYSKTNKSTVAPQTPLPSQIPPHSSLDELKVEIPSLNMESEADLASVPMDLLNIDLSIGRWDNKRMYKIFDFALVGEKFEQLSEKYLVTLATQTSVERLHFLTQVAKHWTGPISVAVFASGADEFRILQHYITYLRLCYDNIRDNISWHLAMPKERIPNKVKIDSSQSFECDSPEKILKNLLSTRSLETIKWRLKNVYPQNIMRNLARKGCQTEQVFLTDVDIIPSVNMTDSLDSFLRSVTTNGRHVSKIAYVIPTYELDLRVKFPESKPELLRLVKKGLARPFHEKVFIYNQFATNFTSLERIDSSSSGFRHNLITTNRSSPFGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H093</accession>
    <name>T1H093_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H093"/>
    <dbReference type="STRING" id="36166.T1H093"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009566-RA">
        <property type="protein sequence ID" value="MESCA009566-PA"/>
        <property type="gene ID" value="MESCA009566"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_456563_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040112">
        <property type="entry name" value="WetA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22934">
        <property type="entry name" value="PROTEIN ESC1/WETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22934:SF23">
        <property type="entry name" value="ZF-C3H1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009566-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="598" mass="67115" checksum="6CE0CF45877F0134" modified="2013-10-16" version="1">MNIDDEHPNEPHPLEFINEPELRKPNVLEVSYFQDSTNVIPERKPAKTSYLEKIKTRVRANFKSSSPDKDCGTFTSITTSGVLAKRTVLNSAPATGRRKNSLSKSQIDSSEYISRERGLTDHQKSEYRLNVFTNQTDSQLLSSLESDVASLKTAQTKNDWIQEWARNARARSVATDCNYSNEYDYSSSDPNLAKLMSPRPPKSPTKIPSPMHTTRLASARTRASLQNLIGIELKLKSEELPQSQQPNITKKSSAHTTPGLSLEHKRNLSLDYHIPNRNQSHQLSPRNRAIPKQQNKYTIDQTTSPLRRSSSFSVKPLSASSSSMVTPNGRRTYQPQAPAPVRRGVPLQKSASSNSFRKMYSDYDDNVEYYINDEDDLIDNDYYSSDEEEEFGATSGDYYDDEELELDQEQQKVQQPQAPTSAPLGGCRNTPEMPRRNVNSAGVRSSMRQSMPRDTSLNRLKNEPSCVPSSLASAKKQLLQSANTSNSNARVQPKYMDISKYKPAQSANFLRKNEAKSTLKPEIKRSPSNVSSTTVNSGFSRNDSARISNRSVKSAGYKPSLQGNNNNRMRDSSGEEWTDSLVINDFNLVIFTDALNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0P5</accession>
    <name>T1H0P5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128530">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128531">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0P5"/>
    <dbReference type="STRING" id="36166.T1H0P5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009728-RA">
        <property type="protein sequence ID" value="MESCA009728-PA"/>
        <property type="gene ID" value="MESCA009728"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631251_0_0_1"/>
    <dbReference type="OMA" id="DENCSIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF91">
        <property type="entry name" value="FI01817P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="4">
        <location>
            <begin position="52"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18633" checksum="9379820349EB9745" modified="2013-10-16" version="1">MIELQMTSTNLDFWLLSRNQSIVSVSPGMQNVFEDRLKDLEAPYDIDDLISNDENCSINGEYYINSIAARYPEYVRLEIMGYSTEGRAIPGISISIHGHHRERKIAYIQGGAHGREWICTPTILYTVSEILANIHAFRSILSDTRLYFVPLVNPDGYEYTHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H117</accession>
    <name>T1H117_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02156043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H117"/>
    <dbReference type="STRING" id="36166.T1H117"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009868-RA">
        <property type="protein sequence ID" value="MESCA009868-PA"/>
        <property type="gene ID" value="MESCA009868"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2580682_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009868-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8698" checksum="FDAFB78146BC41B7" modified="2013-10-16" version="1">MAATQPTSSSAAAATTTTSSLSQEDETIIHNQSQENYKVPSTSKVSVDKLIRVGYYEMEKTIGKGNFAIVKLASNIVTKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H178</accession>
    <name>T1H178_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ARF7 effector protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H178"/>
    <dbReference type="STRING" id="36166.T1H178"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009932-RA">
        <property type="protein sequence ID" value="MESCA009932-PA"/>
        <property type="gene ID" value="MESCA009932"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105549_4_0_1"/>
    <dbReference type="OMA" id="KANMSYS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029264">
        <property type="entry name" value="ARF7EP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46536:SF3">
        <property type="entry name" value="ARF7 EFFECTOR PROTEIN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46536">
        <property type="entry name" value="ARL14 EFFECTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14949">
        <property type="entry name" value="ARF7EP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARF7 effector protein C-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009932-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="102" mass="12259" checksum="7A0240D389249A00" modified="2013-10-16" version="1">MLRNIKKANMSYSQYRDERRGRKNPKQQNLYDEYGRMKEYDFLDICDCMSEECPGCWGICPKCKLSEKCGPVCRKNRNFYFESISTDGKEEVINNEYFQQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1D7</accession>
    <name>T1H1D7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1D7"/>
    <dbReference type="STRING" id="36166.T1H1D7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009996-RA">
        <property type="protein sequence ID" value="MESCA009996-PA"/>
        <property type="gene ID" value="MESCA009996"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220893_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009996-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="24861" checksum="D618A9F3F015A10B" modified="2013-10-16" version="1">MPILPIIPSVNTCMSLTTTTTTSCTIAGNSKPSPAVPEFNTLQLQAFADVCSTVNTSTTMSNSVMNSLVSATKIVLNDNIPNTIASIATTNIEIPKKIPISTIPVPVMKIGSTHFIQDENNESNSLEMTEDIKGKDPQFMNEEDEELSELKIENEKKDLKLIEIAKGREENEIIDDENLEEPMECGSSVASQTSPNNMVKTLSEDVIMSDTLKNEIVEMSDEETLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1E4</accession>
    <name>T1H1E4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Frizzled/Smoothened 7TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1E4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010003-RA">
        <property type="protein sequence ID" value="MESCA010003-PA"/>
        <property type="gene ID" value="MESCA010003"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3071065_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Frizzled/Smoothened 7TM" evidence="2">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010003-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="53" mass="5844" checksum="DFAE4DEF56625E98" modified="2013-10-16" version="1">MNPQLSVQQMYWNNLAQLAAWGLPAVQTVAVLVARVVDADELLESFGKEDPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1F3</accession>
    <name>T1H1F3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373489">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1F3"/>
    <dbReference type="STRING" id="36166.T1H1F3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010012-RA">
        <property type="protein sequence ID" value="MESCA010012-PA"/>
        <property type="gene ID" value="MESCA010012"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294831_0_0_1"/>
    <dbReference type="OMA" id="IICTSKF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577800" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="28"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11228" checksum="C8460BD8DD1279A4" modified="2013-10-16" version="1">MANIVKHMVDLVFACILSLPIICTSKFCELGNFSIHMTLRNLRPAGSKVRKIPVPDANPLSKLFNFVSCPNYTYEIGAWLCFSLMTKCFPALLFTAAGLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1I7</accession>
    <name>T1H1I7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1I7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010049-RA">
        <property type="protein sequence ID" value="MESCA010049-PA"/>
        <property type="gene ID" value="MESCA010049"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624799_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010049-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8917" checksum="07FCE9059084A5F8" modified="2013-10-16" version="1">MQKPCGDREKKFLREINMSDVAAATIVESTKEQKITEAKELYSKGSRNFLVKSYDEAADQLSQCAPYTRNFMENFATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1M6</accession>
    <name>T1H1M6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1M6"/>
    <dbReference type="STRING" id="36166.T1H1M6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010091-RA">
        <property type="protein sequence ID" value="MESCA010091-PA"/>
        <property type="gene ID" value="MESCA010091"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582573_0_0_1"/>
    <dbReference type="OMA" id="WCEGVNI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11801">
        <property type="entry name" value="SH3_JIP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000032">
        <property type="entry name" value="Putative C-Jun-amino-terminal kinase-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047178">
        <property type="entry name" value="JIP1_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="97"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="19058" checksum="AA403ADCD4CEDF34" modified="2013-10-16" version="1">FHNAFAWTNQFSRGTQTPQTHLYSSTSQRNFLSLKCGYLLDEDSSPDSERLQSLGDVDSGHSTAHSPTDFKSMSPQITSPIAQSPIFGGVPFSQLEMLEATHRGLHKFIPRHHDEIEIEIGDPIYVQKEADDLWCEGVNIRTGRQGIFPSAYAVDMDYNDFDPTQQQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Z1</accession>
    <name>T1H1Z1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02132988">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Z1"/>
    <dbReference type="STRING" id="36166.T1H1Z1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010216-RA">
        <property type="protein sequence ID" value="MESCA010216-PA"/>
        <property type="gene ID" value="MESCA010216"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020840_9_2_1"/>
    <dbReference type="OMA" id="MNDESWI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Post-SET" evidence="11">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010216-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7171" checksum="36094EA044090D17" modified="2013-10-16" version="1">MVQEQMPNCYAKVITIESEKKIVIYSKQPIGVNEEITYDYKFPLEDEKIPCLCGAQGCRGTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2G4</accession>
    <name>T1H2G4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02372880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2G4"/>
    <dbReference type="STRING" id="36166.T1H2G4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010409-RA">
        <property type="protein sequence ID" value="MESCA010409-PA"/>
        <property type="gene ID" value="MESCA010409"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_960718_0_0_1"/>
    <dbReference type="OMA" id="CNIQDIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF168">
        <property type="entry name" value="GH22922P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577947" evidence="3">
        <location>
            <begin position="20"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010409-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="33223" checksum="DF04B4BB7C4EC58D" modified="2013-10-16" version="1">MFLKIIIVFAFFLWIGGNCENIEILDICDIIQDSSIGVSCNIQDITEDKTLITTYREGNYYARFLRIENSSNPNIPNNIFGSFQQLVTANFDFCNLNTLKSENFENANNLLELSIQGNNLRQLTSEVFSNVKALVKLELQKNQIEDLQQGCFNGIRFLKDLNLSGNRIRTLNANIFNDMKNLQVLDLSNNKLEGINRNLFKENSKIQVLDLSFNNIAYIHGSTFTHMNLNILDVSNNHFRKLVVHNCKNLYTSNSGIVEIVFKSREAMNELIANDNEIEQLSLSTKKYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2R7</accession>
    <name>T1H2R7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNAJC9 HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2R7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010521-RA">
        <property type="protein sequence ID" value="MESCA010521-PA"/>
        <property type="gene ID" value="MESCA010521"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545188_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNAJC9 HTH" evidence="1">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010521-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9742" checksum="1ABEB5462043498E" modified="2013-10-16" version="1">MTFAHLWKPPIYSQKHIHDLCCSIDTVPFTLRDIQQYKAKYVGSNTERADIKAAYLKHKGCLIHMSADILFMDKYSEPRSRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X5</accession>
    <name>T1H2X5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2X5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010581-RA">
        <property type="protein sequence ID" value="MESCA010581-PA"/>
        <property type="gene ID" value="MESCA010581"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375143_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10799" checksum="980E2C42F545826E" modified="2013-10-16" version="1">MKQSCLKATSKFCKSFTKKRGGGPTPRQIIGILNSLKSSTNSSNLLMVCEIFLILLGMSFRPTSEPQMFTSPLEYKYIINLWKLSANWEIFLSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H333</accession>
    <name>T1H333_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chorion peroxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H333"/>
    <dbReference type="STRING" id="36166.T1H333"/>
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        <property type="protein sequence ID" value="MESCA010645-PA"/>
        <property type="gene ID" value="MESCA010645"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1457936_0_0_1"/>
    <dbReference type="OMA" id="DEHCEAT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF106">
        <property type="entry name" value="CURLY SU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010645-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21445" checksum="B0C2665412EA2138" modified="2013-10-16" version="1">RPLHLKHPYCNEIRVPDDDYFYRLFNVKCIDFVRAFPSPRPGCKLGSRTPFNTLTGVIDANTVYGVTEKFAHKLRTGFGGLLRMNPVFEEYGLKDLLPLKLDIPDEGCTRPNKSMFCFEAGEIRVNEQLVLTCMHTLMAREHNRLANGLALVNPHWDDEILFQEARRINIAIVQHVTYNEFLPILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3I6</accession>
    <name>T1H3I6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospholipase A2-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3I6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010814-RA">
        <property type="protein sequence ID" value="MESCA010814-PA"/>
        <property type="gene ID" value="MESCA010814"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3175943_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="1">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010814-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="47" mass="5203" checksum="7DECF86514D70A18" modified="2013-10-16" version="1">MLFSCLKKTNTSASQLMGSIYFNLVQVPCLKNGKDGGLSYRQAREGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H407</accession>
    <name>T1H407_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H407"/>
    <dbReference type="STRING" id="36166.T1H407"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011005-RA">
        <property type="protein sequence ID" value="MESCA011005-PA"/>
        <property type="gene ID" value="MESCA011005"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873115_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011005-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="36325" checksum="3EF47F99DF654F34" modified="2013-10-16" version="1">MGQYNSTDTDPGDHHSIQRTDQDEEYMQYQKALLTRSPNLHGIRESLSFKRTTSGQSLNTLSMEQEDLLNQVNLRDELLNCEHKELFQFLQEDTENSNSYANYHTCEADTDSLLLDSKKEGSVFSPPRKASTSSLKSNLSYLSNSILQQLEQKQNSIAESIDKVFGRGDNSSNGHKLDEEPLVGNSEFDNIIESFERELEEIKQSTTSLERRLSTLSEPSPIEDDPSKTICSFLKDIESASITSGVQMRQNGSSSRSSVKLKRRSLEKQKKIDDDFNVANEIRKICDELQKPCMEMGATSPFQRRKHDPFSTTFDKIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4N9</accession>
    <name>T1H4N9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4N9"/>
    <dbReference type="STRING" id="36166.T1H4N9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011255-RA">
        <property type="protein sequence ID" value="MESCA011255-PA"/>
        <property type="gene ID" value="MESCA011255"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_4_1_1"/>
    <dbReference type="OMA" id="KLGMWEA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF155" evidence="2">
        <location>
            <begin position="135"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011255-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="355" mass="41299" checksum="792092B359812C1E" modified="2013-10-16" version="1">MRFLKSVVNLGLQSVNRITIQRNALVTVPQITVRWKSTDPASRPLQNIALQPKKRLVRSKKFGLSEEEQLKADGFLNVSAYSTAEEYDLEGLKDALKEQNLYSTKKLISTDSLGVEHDVLYVTANYQVDNEPRDIFFFREGSVVLWNCNEIESNNVLSFLRSFEKDKDNAVAKFRNGQIFLTGDENDFLEKYTFSNAIATSTKLGIWEANLERYIESMEYITEDLKKGRKLKITRSEVLRKTGELFALRHMINLSSDLLDTPDFYWDREELENIYLSVCNYFSIPRRTKVMNEKINHCLELAELVSHNLNDAHHIRLEWMIIILIMVEVGFEIVHYIDRYYSNDDEDKQAVVSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H598</accession>
    <name>T1H598_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02050008">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050009">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H598"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011474-RA">
        <property type="protein sequence ID" value="MESCA011474-PA"/>
        <property type="gene ID" value="MESCA011474"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830475_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF16">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin conjugation factor E4 core" evidence="6">
        <location>
            <begin position="38"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011474-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="335" mass="39124" checksum="A5408B0D40C1A37C" modified="2013-10-16" version="1">MCSQCNPNWSLQFRPNIWDLCSQKCTPQTQTLPLFYCHRLADLKEELGKFKEESLRNTLIEPKNGENLLKFFECSALWLTQITVKTEEDLAQMAAANSYAPKDQKNVSLPIPDYVPHYLRSIPEFLFTNIVNYLEHRRLYEDGGIRLPIXXXXXXXXXXXXYEDGGIRLPIDAQNEIFRWILIFMGSIKRTKSPHLRAQLAVAFRCLLPDPSVNSFQIHVFDNHPDRYQIVQNLLNVFVAIEMTGQSVEFELKFAYRRPMYAVMKFLWKREDQKTCFNESWTTMSQTDQQTLQRNLNHLTRQTATYNTLSKETIGMLVLITSEIKDIFCHASMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5A6</accession>
    <name>T1H5A6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminopeptidase N-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5A6"/>
    <dbReference type="STRING" id="36166.T1H5A6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011483-RA">
        <property type="protein sequence ID" value="MESCA011483-PA"/>
        <property type="gene ID" value="MESCA011483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3338056_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="38" mass="4591" checksum="A59D1EACE2599E66" modified="2013-10-16" version="1">MDDDMHGFYRSNYIDDNGNEKWLGSTQFQRNHARRAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5F8</accession>
    <name>T1H5F8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5F8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011535-RA">
        <property type="protein sequence ID" value="MESCA011535-PA"/>
        <property type="gene ID" value="MESCA011535"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1333272_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011535-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="206" mass="22790" checksum="19DB570DF15B3144" modified="2013-10-16" version="1">MDISKIAEENKTEEKVALPISSETPDDTKNDVKPSLNGDSEQTPQNSEQKVEEVAEENKINEKETVALNGAKEEIQEPIEVPSADKVVEEGIKLNGNVSEHESSKIEVNTKNDIITDPPTDTNTNTSAQTPPPLPLSPQPTQVMVFALSDNNEQHQQQEKENVEQEPKPLPTDLNVTPQLQIPSVIETAPTPEIEKREDTMKLKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Q0</accession>
    <name>T1H5Q0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5Q0"/>
    <dbReference type="STRING" id="36166.T1H5Q0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012033-RA">
        <property type="protein sequence ID" value="MESCA012033-PA"/>
        <property type="gene ID" value="MESCA012033"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3399819_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012033-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="31" mass="3798" checksum="D0AEC2D299395E14" modified="2013-10-16" version="1">MESMEYEMVRNLTILFFLERLLDKGEPRTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Z0</accession>
    <name>T1H5Z0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5Z0"/>
    <dbReference type="STRING" id="36166.T1H5Z0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012123-RA">
        <property type="protein sequence ID" value="MESCA012123-PA"/>
        <property type="gene ID" value="MESCA012123"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3244923_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012123-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="43" mass="4739" checksum="B6AB3836EAA419A6" modified="2013-10-16" version="1">MQGKSLKNGEITQEIKDMSRTYRLLGSDKVVALESQLVKSGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H614</accession>
    <name>T1H614_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein G</fullName>
            <shortName evidence="9">snRNP-G</shortName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H614"/>
    <dbReference type="STRING" id="36166.T1H614"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012147-RA">
        <property type="protein sequence ID" value="MESCA012147-PA"/>
        <property type="gene ID" value="MESCA012147"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_10_1_1"/>
    <dbReference type="OMA" id="MSKAQPP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01719">
        <property type="entry name" value="Sm_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000017">
        <property type="entry name" value="Small nuclear ribonucleoprotein G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034098">
        <property type="entry name" value="Sm_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF2">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012147-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8535" checksum="E70449C8421BAACE" modified="2013-10-16" version="1">MSKAHPPELKKYMDKKMTLKLNAGRQVTGILRGFDPFMNVVLDETTESCKDGTKNHIGMVVIRGNSIVMVETLDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H636</accession>
    <name>T1H636_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Autophagy-related protein 16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H636"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012169-RA">
        <property type="protein sequence ID" value="MESCA012169-PA"/>
        <property type="gene ID" value="MESCA012169"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013923">
        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08614">
        <property type="entry name" value="ATG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="1">
        <location>
            <begin position="28"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012169-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17642" checksum="C1BB696DD825729B" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKKLLSQQEELTELHRRKGENSQRIVDLNIKIEERQLINVKDRNASFRAEIKMYVTSMEELKRLNTTLKDEHTALQLAFSSLEEKHRSIQEENYSLLERLKDYKSRDADKLNEENESFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1H642</accession>
    <name>T1H642_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aspartate aminotransferase</fullName>
            <ecNumber evidence="14">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="14"/>
    <dbReference type="STRING" id="36166.T1H642"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012175-RA">
        <property type="protein sequence ID" value="MESCA012175-PA"/>
        <property type="gene ID" value="MESCA012175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_3_0_1"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="15">
        <location>
            <begin position="145"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="429" mass="47297" checksum="885E1FC25DAE1582" modified="2025-10-08" version="2">MXXXXXXELFAAPINAKIIVWNGPPGVFEFPNFAKGTKSIMDTVVEATKNGTVSIIGGGDTASCCAKWGTESQVSHVSTGGGASLELLEGXXXXXXXXXXXXXXXXXXXXXXXXXXXQWFNSIQMGPPDAILGITEAYKRDSNPNKINLGAGAYRDDNGNPWVLPSVRKQMDHEYAPIGGSAEFSKLASELALSKNSNRLKEKCNATVQTISGTGALRVGASFLSKFWPHNREVYLPNPTWGNHVPIFEHSGIKVQRYTYYDPKTCGFNFQGCLDSIKNIPEKSIILLHACAHNPTGVDPTFEQWKELSALIKSKQLYPFFDMAYQGFASGDIDRDAQAVRLFEKDGHEYCLTQSFAKNMGLYGERAGAFSVVTSNKDEADRIMSQVKILIRPMYSNPPVHGARIVTEILSDDELRSLWLKDVKLMADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6A2</accession>
    <name>T1H6A2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6A2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012235-RA">
        <property type="protein sequence ID" value="MESCA012235-PA"/>
        <property type="gene ID" value="MESCA012235"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009579_15_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035237">
        <property type="term" value="F:corazonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230:SF163">
        <property type="entry name" value="CORAZONIN RECEPTOR, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="351" mass="39406" checksum="BF7A89677B4B15CE" modified="2013-10-16" version="1">EHAPKLLKSTVIKVIVLSAMAIISLLGNIATMWNIYKTRMTRRSSRHSWSAIYSLIFHLSISDVLVTGFCIIGEAAWSYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSEKMFQGSKLAVYTNKPSVQTNRQRLIHKAKMKSLRISVVIIVAFLICWTPYNVMMIIFTFLNPDKRVGEELQSGIFFFGMSNSLVNPLIYGAFHLCPIGKTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6T4</accession>
    <name>T1H6T4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plus3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H6T4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012417-RA">
        <property type="protein sequence ID" value="MESCA012417-PA"/>
        <property type="gene ID" value="MESCA012417"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018644_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.200:FF:000001">
        <property type="entry name" value="RNA polymerase-associated protein RTF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Plus3" evidence="9">
        <location>
            <begin position="196"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012417-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="543" mass="62971" checksum="DB359E8972B796E3" modified="2013-10-16" version="1">SHSNSSSGSESSSDSEFDDGFDENYYGDEEDRARLNALSEKERETEIYKRTERRDMMRTRWEIERKLKQQRRNEKGAKSSSKKAKNLRKRRNLLKKKIAKVTPAAAPIVHPTPVEPIPEIVRQVTENHLLGGEKDNAQEYFDPKERSKERKKNVEMNRTDDKRXXXXXXXXXXXXXXXXXSSDSEAEEKQKKAIYISSREQLNQLRLSRYKMERFVNLPIFERVVLNCFVRINIGNNAQNQKPVYRVAEILGVVETAKIYLLGKIRTNLGLRLRHGTQERVFRLEFISNQEFTESEYHKWIEQCSTQNVTLPTLDLIDKKKKDIAEALNYEFKDEDVDRIVAEKNRLRNRPTNYAMRKTMLMKERDAALLRGDNDLAKELGEEIDGLESRASELDKKRSSSISLISYINDRNRKRNVEEAEKAIMEEARAAKGMKIMDPFTRRSTQPRMSFKASDKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTVKVIPKAIPKTNEVAPKRALNLEDYKKKRGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H737</accession>
    <name>T1H737_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H737"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012519-RA">
        <property type="protein sequence ID" value="MESCA012519-PA"/>
        <property type="gene ID" value="MESCA012519"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232548_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003949">
        <property type="entry name" value="K_chnl_volt-dep_EAG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01464">
        <property type="entry name" value="EAGCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012519-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="225" mass="25309" checksum="4FE412870F091998" modified="2013-10-16" version="1">TPLWLLLQVAPIRNERDLVVLFLLTFRDITALKQPIDSEDNKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSKFAKLARSVTRSRQFSAHLPTLKDPSKQSNLGHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLNFHTTFVGPGGEVVSDPKVIRMNYLKSWFIIDLLSCLPYDVFNAFDHDEDGIGSLFSALKVVRLLRLGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H776</accession>
    <name>T1H776_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H776"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012614-RA">
        <property type="protein sequence ID" value="MESCA012614-PA"/>
        <property type="gene ID" value="MESCA012614"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318298_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23159" checksum="6675574BE338E938" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLVDRILARAAASKEVRADDNEETLKTRIATLKNTNDILAQYTEKTAVINAERPVDEIFADVVTAIDGVLAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7J1</accession>
    <name>T1H7J1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of split malpha protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7J1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012728-RA">
        <property type="protein sequence ID" value="MESCA012728-PA"/>
        <property type="gene ID" value="MESCA012728"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106880_0_0_1"/>
    <dbReference type="OMA" id="ADRDLCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029686">
        <property type="entry name" value="Malpha/m4/m2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12254:SF0">
        <property type="entry name" value="BARBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12254">
        <property type="entry name" value="ENHANCER OF SPLIT MALPHA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15952">
        <property type="entry name" value="ESM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="17439" checksum="15058B3EF0947842" modified="2013-10-16" version="1">MSFIAREYRFDNSMTMKYQEILAVKGLKKVLKPIMKIIKKQQFNLKTIDDFQTDNDVNASWEDMQNENYANEMLEQRIFNEINQCEDDAAMYVVQGNRRHIVPVQKTQTFVPVNFVRTESGTFFWTSMETIQQQQFYSPRDRWAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7K9</accession>
    <name>T1H7K9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7K9"/>
    <dbReference type="STRING" id="36166.T1H7K9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012746-RA">
        <property type="protein sequence ID" value="MESCA012746-PA"/>
        <property type="gene ID" value="MESCA012746"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3379669_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012746-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="34" mass="3853" checksum="D164D480D1E8E2D3" modified="2013-10-16" version="1">PNNVLSGMSSFDCESLPDYSEFDSESVTLDYFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAR4</accession>
    <name>T1GAR4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAR4"/>
    <dbReference type="STRING" id="36166.T1GAR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000324-RA">
        <property type="protein sequence ID" value="MESCA000324-PA"/>
        <property type="gene ID" value="MESCA000324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_287185_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001384">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004102">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="488"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="796"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1074" mass="123309" checksum="CF029F3CBBF022FE" modified="2013-10-16" version="1">MFPKIREYNSRNDILENQISLSLICEIIFNDIKIEKEKLNKISAISYSHAVSLNLINDKLNNVFLRNLYEFLNEIKSEIKSIIPLKEITTTTYIKTLISFLWIVTALRAGEFISLEHVNFSSLAVLDRLNPIFEPNGKLLISEKGVSRSDECEVIEKHDNFQAFLTIDPKNGELSRAMRNRCIELALCTDNYTLDDLRSIIYINGIHDMNLIKTVLNIHNEINSLSEIQFFTISHLSKFSFLLSSYLKIGSSYLESMHYSVIEVYVNSCSVDLLGFGLKYYKNKVDEIIKINMEKQNFDKICLGKLCLTNITSLIDPVEKNRIKKEYIFEDIDILNDLHSSYKLFQTIMNFNGLGQELDQEISKQIEVLEDKKQKYDKKLAIRPKECLYGAVTEEIRYFLTSNGNPKNLLSLIDLVESKYKALHENEKCPEISDLNKRLDLWITNSQMFLHQLTTKYASYYKDLTRPVECALNTICFEFQRLKYKLEFNEPVLLVGPTGCGKTTVCQILSKIYKKNLRILNCHMHTEAADFLGSLRPYRGEKNKESSEKHRFFEWSDGPLILSMQEGNFFMADEISLSEDSVLERLNSVLEPERTLLLSEKGGFNNTEINIIKAHHDFKFLATMNPGGDFGKKELSPALRNRFTEIWCKPPENREDLINIAANALQEIVSKKKSVKYVEQVSTLLIDVICFMSEEIDTFKFSIRDILSWCNFFLVNKHLPFPIVSILGFETIFLDSLEMIELNSLKLNSIKCEENLFGIYPFYISKNESTTDNKDFLFGAPTTKQNLFRLLSAMSLKKAILLEGPPGVGKTSIVESLAKAVGFKIVRINLCEHTDLSDLFGTDLPAEDTDLDANNTGSFVWKDGPLLAALKSENTWILLDELNLAPQNVLEGLNAVLDHRREVYIPELNKTFVVGGENRIFACQNPLRQGGGRKGLPLSFLNRFTKVFLKKLSDGDLLFVLKDLYTEFFAKVKDTFSISLAEQMVKFSGLLATGISNLEFGYKGGPFEINLRDILRWCDSLTHPDTGFIFEVESNNLEKFYGVLFDRMKLIYYQRMRSAKDKEYILRSFSNVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB03</accession>
    <name>T1GB03_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CWF21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB03"/>
    <dbReference type="STRING" id="36166.T1GB03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000422-RA">
        <property type="protein sequence ID" value="MESCA000422-PA"/>
        <property type="gene ID" value="MESCA000422"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867608_0_0_1"/>
    <dbReference type="OMA" id="WETIDPQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21370">
        <property type="entry name" value="cwf21_SR140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047488">
        <property type="entry name" value="SR140_cwf21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CWF21" evidence="3">
        <location>
            <begin position="150"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000422-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="321" mass="36227" checksum="BA15DC6D6322294C" modified="2013-10-16" version="1">FIEKKEEPEDIDGAPLSGDEKDDEDLDGVPLDGAALLKSAMLRGMSDSTPKTPATSRIVVLSAIMMTMILMVFLVMDDDIDGIPIDSKDLKGGSGGFIPSKWETIDPQQVQAQAITTSKWDTLEPPEAPKFFNSDDDSDSNPDQSRNINEDHRSKLRDIEVRTMKYQDELESGVKDLKSGWSIDEQVEQYRRKLLNSRKRSMSPSESSKKMKKSRHPSPTSSHSSRSSPGRSLKRRSRSPKDSSRSRRNKSRSLSNSPRRDKYKERSPVYGKSSRYDSPMQRRRSPSPTPSRGRRELSPRSSSSRGASPSSSRKHKHKHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB31</accession>
    <name>T1GB31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zasp-like motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02025492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000452-RA">
        <property type="protein sequence ID" value="MESCA000452-PA"/>
        <property type="gene ID" value="MESCA000452"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600761_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576778" description="Zasp-like motif domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="52038" checksum="5DF05812C045F986" modified="2013-10-16" version="1">MKILSLIFCMCFGFSYAVKVNIDQTYDNFEVYAKDKRQSGNQDNIQQRQLVYIPHSLNRNEAVNKCSYLYGNKYPQEDPIENVHAPTRRIIEHQSNSNINRHSTKNSNVFLPIEHRQESENLQRLIPEEQLLKLIEDELAARGFPLASRPFPTPNHLQQTSGQNIALEIANKAREILPAYQTQSGNFVPILSPNYGYQNIDHNSGKLESKANNVNKASKVEESKYNNHFNQNPNQKLQQTNIQSTPLPSFLGHYNYLPFAQLNGYSTTPKSVKAAFTATLPKQLNIENRLLKSTAPQTFIGFNNNNNNNNNENGAGPVAHPTPRNNPYFSSQQYINPYIENFQKKAPVSKKDSIINPTVIQRQEYQQNDEQQLSPTKVPSNSHPEEFQALVDAGYPVTAVPVPVPYDEYIKQQQRLIEIPLKPKQQVKLTQSRLQNVLEPVHIQQQKQQEHNGGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB49</accession>
    <name>T1GB49_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dedicator of cytokinesis N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB49"/>
    <dbReference type="STRING" id="36166.T1GB49"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000470-RA">
        <property type="protein sequence ID" value="MESCA000470-PA"/>
        <property type="gene ID" value="MESCA000470"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909072_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dedicator of cytokinesis N-terminal" evidence="1">
        <location>
            <begin position="54"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000470-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15630" checksum="C6A5ABEA52A4F2B7" modified="2013-10-16" version="1">MSFYIYNIVLVGTDMTNSYIYVDEQICDTLGKFIDNESSSFLSPFFVFILITYDREPYKFNAIQKGMITLLERRRDLISSTLTQDQIAEMRMIIISKIDWGNSILLLFRTFPLQDKLFAFLMSKALMEFLQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB79</accession>
    <name>T1GB79_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000501-RA">
        <property type="protein sequence ID" value="MESCA000501-PA"/>
        <property type="gene ID" value="MESCA000501"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760734_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588151" description="Secreted protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000501-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="8152" checksum="D1C50CAB6D85B7E8" modified="2013-10-16" version="1">MIIVLIPLSISFLVQSCCVERWWFVTVFLPNYGTTPFMKCYEPEAITRVKYIAITGAHEKTARYIYDCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBL2</accession>
    <name>T1GBL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190540">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBL2"/>
    <dbReference type="STRING" id="36166.T1GBL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000646-RA">
        <property type="protein sequence ID" value="MESCA000646-PA"/>
        <property type="gene ID" value="MESCA000646"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688080_0_0_1"/>
    <dbReference type="OMA" id="CEEYDEH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000007">
        <property type="entry name" value="Low density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF5">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="188"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="288"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000646-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="401" mass="44816" checksum="EF364A2C2B6CF292" modified="2013-10-16" version="1">RDGTGRQTVLPPIEPYDPNASDWLGGIAIDWIADNIYWADSKRNVIEVARLDVQGYLFYVGDNKIGRIALDGSQPFILVNDTKTVSSIVLDIDKQKVYWTEMLSNMIWRVDYDGNLKTPKLKSSLSNPIALGLLDNYVYWADNTHNNGSICVAPDSDLKNFKQILFSEGAPIRDLKIFSSRIQSGYNGCAVKNGGCSQLCLFNGTNTICACSHSKVGRDGKSCEEYDEHLIFSYGSAVESIHLTDHTDKNHPIKKIQDTNFMRSTIHRPMDLKIYHPYRQAQPSSGNPCENANCSTLCLLTPDEPHYRCVCPNNFILGPDQKSCIANCTSAHFQCATTYKCIPFHWRCNKVDDCGDNSDEPDNCPEFLCSHGEFQCASKKCIQPSMICDGVSQCRDGSDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBL6</accession>
    <name>T1GBL6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the JMJD6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBL6"/>
    <dbReference type="STRING" id="36166.T1GBL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000650-RA">
        <property type="protein sequence ID" value="MESCA000650-PA"/>
        <property type="gene ID" value="MESCA000650"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016785_8_0_1"/>
    <dbReference type="OMA" id="ESWAANE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033749">
        <property type="term" value="F:histone H4R3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106140">
        <property type="term" value="F:P-TEFb complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF32">
        <property type="entry name" value="BIFUNCTIONAL ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="13">
        <location>
            <begin position="148"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000650-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="33310" checksum="9E03AC7D22CB7C31" modified="2013-10-16" version="1">MSSSEVKRLPKRSRKRVREVKRKARPELGTKESWSALKYAERFEAYKQFTDNVERIRESEVDCQTFIEKFEKPYLPVVIQGCQESWAANEKWTLQRLTKKYRNQKFKCGEDNEGYSVKMKMKYYIQYMESTKDDSPLYIFDSSYGEHHRRKRLLEDYTVPTYFQDDLFKYSGEDRRPPYRWFVMGPARSGTGIHIDPLGTSAWNALVFGHKRWCLFPTNTPKELLKVTGQMGGKQRDEAITWSVLFTQGLKWLIGLQSSSLWKSYRNLEKLYLCLEVGGMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBU2</accession>
    <name>T1GBU2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBU2"/>
    <dbReference type="STRING" id="36166.T1GBU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000731-RA">
        <property type="protein sequence ID" value="MESCA000731-PA"/>
        <property type="gene ID" value="MESCA000731"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_32_1"/>
    <dbReference type="OMA" id="NCETTRF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF152">
        <property type="entry name" value="UT01108P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="32"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000731-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="23542" checksum="6465979FC2446316" modified="2013-10-16" version="1">MPYHEIMRALKEEFTAKWNDRQPSPQGGLEDHEQLSVLGSGAFGTVRLCRHKQTNEIVAVKYLIKKDVVKRKQIKHVVNEKQVLKSIYHPGVVSLVFSRKDIDLLYLAMPFVNGGELYNYHKKLVKFNESQGKFYLSQVFLALDYLHNMDLIYRDLKPENILIDSNGYIKLTDFGFTKVSFQILNIHIQYNICQIYSHWFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC02</accession>
    <name>T1GC02_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02006258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC02"/>
    <dbReference type="STRING" id="36166.T1GC02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000795-RA">
        <property type="protein sequence ID" value="MESCA000795-PA"/>
        <property type="gene ID" value="MESCA000795"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_7_0_1"/>
    <dbReference type="OMA" id="HNEYRNF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034763">
        <property type="entry name" value="P14a_insect"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038921">
        <property type="entry name" value="P14a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SCP" evidence="5">
        <location>
            <begin position="47"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000795-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="27299" checksum="E9C4D39DD6BB832E" modified="2013-10-16" version="1">MYSEDLTAAQPTPMLSSETIAVKCPVTGPPELSSSCPSDAKFIDLTLMKDQIVDFHNDRRNQVAGGKIPKHLPAVRMATIQWDNELAYLAGINVKSCNYEHDECRNTHKFKFAGQNLAKDSWYGSKAQVEVLVLDQIEKWFSESEDSDMSRINNVGSTENPVTGHFTVMVAELSIKVGCAAVRSSEYMEGKKWETLTTACNYAHNNVVGSKIYRSGPAASECTTGTNPQYPFLCSVNESYDPNVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD98</accession>
    <name>T1GD98_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001280-RA">
        <property type="protein sequence ID" value="MESCA001280-PA"/>
        <property type="gene ID" value="MESCA001280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161227_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12341" checksum="55C232068252333B" modified="2013-10-16" version="1">MEGLLSIIQSLSKGPNEKAFPQFVKAYSEHQSLQLQNLSSNDGIIQHITSLMSKQETRQNGYLILTSCLSQIPLDIIEQKSTLWSNLAIKACSQKGSDDIAPLGFRLLSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDJ1</accession>
    <name>T1GDJ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="MESCA001379"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001379-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10799" checksum="DD8FEEC7AC9797C8" modified="2013-10-16" version="1">MKKSQEMELFNFYLRKSKVQNCNIPKTAMLGAKSNSFLQPVIYVNYKIYGCELLAKYGSNYLNTSLYPFMYLDLEVLKLANANSFSLEEGMPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDQ1</accession>
    <name>T1GDQ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA001442"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867446_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001442-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15846" checksum="2C264799F8DF9134" modified="2013-10-16" version="1">MDGGLTYCMKLVARGSLFYPDGDRIIQEFSQFPNPGTRLVASPAISSIENASLIFFGLLRSHKVHIAYIRILNSVQYTITIAKNSKKIDIERDIFYHNINLHVTKYFQEEYFFSERAIARSLKFGTYIHHRPVTRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE78</accession>
    <name>T1GE78_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001636-RA">
        <property type="protein sequence ID" value="MESCA001636-PA"/>
        <property type="gene ID" value="MESCA001636"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173853_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001636-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12894" checksum="3237FB240F87B346" modified="2013-10-16" version="1">MNFSAFRFLVIPWPQSVCPPYVFVQPSKQIFLLYNILLAKNVELIKRLSVPNHEIIHKYTIQRALPKTPNTNFLCRLENDKLKLMLKKKDEELVTAKAAVDRIATTMFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEL8</accession>
    <name>T1GEL8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Ornithine aminotransferase</fullName>
            <ecNumber evidence="6 11">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="6 11"/>
    <dbReference type="EMBL" id="CAQQ02026811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEL8"/>
    <dbReference type="STRING" id="36166.T1GEL8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001786-RA">
        <property type="protein sequence ID" value="MESCA001786-PA"/>
        <property type="gene ID" value="MESCA001786"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_2_1"/>
    <dbReference type="OMA" id="RKWGQLH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001786-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="280" mass="30475" checksum="852EA22F48344034" modified="2013-10-16" version="1">MNTGVEGGETALKLARRWAYSVKKVEKNQAKIVFAANNFWGRTMSAISASTDPSSYEGFGPFMPGFEIIPYNDSSALEKALQDPNVAAFMVEPIQGEAGVVVPDEGYLKKVRELCSKYNVLFIADEVQTGLARTGRMLAVDHEEVRPDILILGKALSGGMYPVSAVLADDEVMLTIKPGEHGSTYGGNPLGSKVAIAALEKRLNKLPKEIVTKVRGKGLLNAIVINKDFDAWEVCLKLRDNGLLAKPTHGDIIRFAPPLVINEEQVLECTDIIKNTILSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEP6</accession>
    <name>T1GEP6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001817-RA">
        <property type="protein sequence ID" value="MESCA001817-PA"/>
        <property type="gene ID" value="MESCA001817"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3089605_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001817-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="6110" checksum="8A10671C8977510D" modified="2013-10-16" version="1">MPFRAMPTKEISQSVNCRKCVWLCPLVARKVEKFKLWNQNTDSDFNTCKFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEV9</accession>
    <name>T1GEV9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02067685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001880-RA">
        <property type="protein sequence ID" value="MESCA001880-PA"/>
        <property type="gene ID" value="MESCA001880"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_499027_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="412"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="476"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001880-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="546" mass="61279" checksum="1EE93A0F01C68379" modified="2013-10-16" version="1">MPYEIFELSVNNENSSGVTTLPSFELSLKDYDAGDEFKLVLLSDIFSIHSLLVTILSTTRSIGHAYISTKQINNSYRGKPTISRDANFHTWNEKWCEFSLNQRRRKFARFAEDAPENTYENITSSNETEVVTEKSLPILKDDVSSTPEKNTSENITLSNETEVVTEKSLPNFKDEISSTPHNTQSVTSSNETEVVAEKGVPILEDEISQTSKNCSFIKKQFNKLLKHVKNTGSDEKEDARNSSLASSNSAIETNTSILRAESVHSKTDDNDDLTLVSTIPPIQMVTRNTDIAVSGASNKTTKGPNNESTEKAFSTTEGMFPLTTMITLYGNDTTIIVGSSGNATESPFYIATRRMLNSSNAPISTTLEKPINLGKYFRAAVNDNPPVSDENPLQATIVSDEPFDVLSPSGSSFSCVTSGFFKDPEDRSCTKYFFCAYKSKPGKFDLYQMRCPEFHQFNVQEQTCVMKQQPSCPTIRKYCERYGRYPDPEDYSCRKYISCFEDFASLYGKIVECPQNMQYNVLQRSCTKERQQGCPLIIDNINQVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFK1</accession>
    <name>T1GFK1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02395051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFK1"/>
    <dbReference type="STRING" id="36166.T1GFK1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002145-RA">
        <property type="protein sequence ID" value="MESCA002145-PA"/>
        <property type="gene ID" value="MESCA002145"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064868_1_0_1"/>
    <dbReference type="OMA" id="VIMIRTR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02962">
        <property type="entry name" value="TMX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037463">
        <property type="entry name" value="TMX2_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="132"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002145-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="343" mass="40410" checksum="65342D3082CDC18B" modified="2013-10-16" version="1">MTFKKDLKLLVKPYYWVNILLSLSYIIAKKTPGVCNYLFFNKEEMCEFGSREAEILFFLAIIVMIRSRKTGSTTMITYLKQSFLYNKGANLILWAWADYLYGVIYLLVFVVMAMLLPEPTYKGPEHVTYFRNAQSFEEELTREKRITWVVCFYTNWNPSCVNFASIFSQLSAEYHLENLRFGKIDVGRFPDSAQKYNINDGSLSRQLPTVILFKNEKEVDRRPSYDARGRLTKFYFSYDNVVAGLGLNNLYKECRENPIKKAKQRNQLPKRRLIRMNFKEVHEKLHISINFTFKKSLLKNFELPNLTHFIHSSHVKRTSNLALNKENLVQHFVITNSKFVQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFM0</accession>
    <name>T1GFM0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFM0"/>
    <dbReference type="STRING" id="36166.T1GFM0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002164-RA">
        <property type="protein sequence ID" value="MESCA002164-PA"/>
        <property type="gene ID" value="MESCA002164"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024950_2_2_1"/>
    <dbReference type="OMA" id="VLPDHGY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF247">
        <property type="entry name" value="PICKPOCKET 23, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002164-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="491" mass="56846" checksum="4D5B6D44064CA8FB" modified="2013-10-16" version="1">MKPKLRKTPTTKSIKSEVVHEAVIFRRSLIYQTKEFFQNSTLHGVRYIAETGRPNGEKFMWFCFTVIGTIAALVIIMSLWEKFQTNPTITGLDTDFHNQQVIFPTTVICPEEGYDETKVTDIAYKMFYDAESGERAEPFYKMLTGLSFDNLDDAFKLSLEVVEMGGSLDEMDLRKTAFDIHVKCEEIMGTASIEMKKLPAAHILIPELTNVQPHDLYETDKKWSLQFVPNRTSKIFLFSYEEHFGGDFKAQLEWAEGQESDILISKKNTYTTDDARQLSIQQRKCIFYNEFNLNYYPGDYTFSACMKECRTRKALQMCKCVPPFFKPVPGKNCRQCELSCSKTVYNIEKLNKNYDKAQDQHVQVEFLTWPIIRYKREVLFGWVDLLVSFGGIAGLFLGACCMVYRNKEILENIEEEIKKKPPKPVDLHVFPKKRRSIREQKVAPVLLGNTDGVLRPVRKPRNQKDFSGYARGFYNDSFVKDGEKRYGGYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFS6</accession>
    <name>T1GFS6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140015">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFS6"/>
    <dbReference type="STRING" id="36166.T1GFS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002226-RA">
        <property type="protein sequence ID" value="MESCA002226-PA"/>
        <property type="gene ID" value="MESCA002226"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3034424_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002226-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6178" checksum="161735394E85F184" modified="2013-10-16" version="1">MSEVTRNKLAAAGGYSIEPRGPIEIKGKGMMNTYWLLGKKGFDKHLPNPPPIGESHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFW5</accession>
    <name>T1GFW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DUOXA family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02078662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFW5"/>
    <dbReference type="STRING" id="36166.T1GFW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002267-RA">
        <property type="protein sequence ID" value="MESCA002267-PA"/>
        <property type="gene ID" value="MESCA002267"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045258_3_0_1"/>
    <dbReference type="OMA" id="FTWGMEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018469">
        <property type="entry name" value="Dual_oxidase_maturation_fac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31158">
        <property type="entry name" value="DUAL OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31158:SF10">
        <property type="entry name" value="LD27791P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10204">
        <property type="entry name" value="DuoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="191"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002267-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="280" mass="32368" checksum="638C77EEACF86325" modified="2013-10-16" version="1">MTKHYHEALRRGLPFPILTVAEYFSLSQEGFTWGMEYRQAGYFASILLWTSLASWMLMNLLLIAIWSIYESIYRCTTSLNNIGYYYLLPKRPLVIFLEGGTLEFSLGWCYWLLLVAGVLCFIVGVAISVMDLVWPHRFSTILEVYFDTPYDRHVILEESSDVRYRKRPTNKGLENPPGLGSRILRRLSSKATKETSLNNNGPNDGIENKGFQGDVPKSPWRYPFRRSQNMPQRNLRRTISQDSTSSIASASVQISPIHKQALSRMLPKTPHERIRDLDHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFW9</accession>
    <name>T1GFW9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFW9"/>
    <dbReference type="STRING" id="36166.T1GFW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002271-RA">
        <property type="protein sequence ID" value="MESCA002271-PA"/>
        <property type="gene ID" value="MESCA002271"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053503_3_0_1"/>
    <dbReference type="OMA" id="MANDEFF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF192">
        <property type="entry name" value="IP17501P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="4">
        <location>
            <begin position="23"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002271-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="25433" checksum="CDF083C11D5B1318" modified="2013-10-16" version="1">MLVDVYGGPDSYSVVDKFGIDFGTHLTTNKSIIYAKIDGRGSGLRGDKLLHEIYLNLGTVEVFDQINVTRMLQKAIPYIDENRIGIWGWSYGGYVSAMALAKDNNSTFKCAASVAPVTDWIYYDSIYTERFMQTPYTNPEGYKSARLSTLAPLFKNKDFLLIHGTLDDNVHYQQSVILAKHLERADIFFQQISYTDEDHGLINVRPHLYHSLSRFFDECFNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG17</accession>
    <name>T1GG17_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG17"/>
    <dbReference type="STRING" id="36166.T1GG17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002322-RA">
        <property type="protein sequence ID" value="MESCA002322-PA"/>
        <property type="gene ID" value="MESCA002322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_341728_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="830" mass="94824" checksum="F4D6BB9345A78655" modified="2013-10-16" version="1">MIYEINGNISLIVPRKNATAGLHRVNAGYMERNTETLELKEIFTFLRASFYAQMKIIEEGYLFIEENLVLPDKIKARTVEIDGDESEIGHIFINQDEWTEEDDQVDLLKLADMIEIFHNDIKLLEDVAENSTSRRKREAPLQFDEMEFDFVEVTDELILMDPGNSSFEADRLEIDGDLSVKIINDKDWKEFEKTLIRMDRNDTLENLVVDGDVIFENELIVENLNDKSLKNEYLWSNIESFETVKVSGLKRFENILTNDIDTDGFINGVRPSDFISLSGNQTIHGRLSSQISWSSKEQSMEEALKIFYQTMIYGQQESLSPDASLIQSNEIIEINSLKTFESLTATKVVVESGELNNIPLENFVSKTRPQELQITNFKGIIVTNFLDIKGLVDGVNPKEFFEDCVYTNDSNSDIHLNITAIFEEDIEADEFQVFKIFNNRPVHDLITTDNLTDHFEKFTFQKLRVDTMDCECDVEGVGILNGVDIRTVKARSFKAPAHPQHLYVDHLVLRKGLNVSRINTIPMETITKYLDSLQDLPEMLKSGRLSVDTIVVKGNALHFEKNMYINGQLNDDSQTFPEFISQIAFKTEDYIQLRGKSEFLYEVLCHDNIFVEEMNGIPFSDIAMKTTPIEFAEDLEIHGNLHVNNLVVKENLNNHSIAELPHLCRFDNYLNTWVFGGPLLFKGHVEIENLILRSSVNDVDDMEELLNSLTYINQISVLKGKTIFKNEVSINKGGFIKHLNGFDLMDVLNNMVFISGSHQVKISGPVVFQGPVKATHVTIKSGLISENINDCDVGEWVEDSVKINEDIKLNGMCFDYISCINKQIRIILNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG83</accession>
    <name>T1GG83_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002394-RA">
        <property type="protein sequence ID" value="MESCA002394-PA"/>
        <property type="gene ID" value="MESCA002394"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017860_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002394-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14100" checksum="59E96496EE78049E" modified="2013-10-16" version="1">MGIILVKTTATGILGMQKLQRLSGWRDEEYDKTVAEKDLLYLKKCPKKHESISKQKEDFFKELLSNEDIPRLTRVEVDKAIQRLKNNKSAVIFEVRAASKSLEGGESGFHDMSMRLLISESWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGD8</accession>
    <name>T1GGD8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGD8"/>
    <dbReference type="STRING" id="36166.T1GGD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002454-RA">
        <property type="protein sequence ID" value="MESCA002454-PA"/>
        <property type="gene ID" value="MESCA002454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_842755_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="330" mass="35823" checksum="ADC7167FC6FAB053" modified="2013-10-16" version="1">MFSNDDQVDFAQSAKKPQNKSTENLLDVGVVRNPTLTKRAMSVENLNDVSRSYFPSRSTNDSFQSLSSEFSQSYASLPRRTVPTLERRTSFSGQPLETETGRKVAVANILEQRRRSLSKLRGLVIPDRPQIPISNLEPILDLPEIKSRDSDKINNKFNGIPASYSTTVVKRETTKTMNSIKPAPTTYKSILANNRLPSGGFKKNSDLVPPVKPPRTSLGVSKSSAFEESDSDSVFSSKVSSPPTSPRLPVTVVPEKFALTRTLSSETNTSIASSTTSTLTSGSGSQASCSSAGSTPAIDMSRRQVKCTTDRCSLVLSTANTSDYGKQHDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGK4</accession>
    <name>T1GGK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002525-RA">
        <property type="protein sequence ID" value="MESCA002525-PA"/>
        <property type="gene ID" value="MESCA002525"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1887868_0_0_1"/>
    <dbReference type="OMA" id="PPTWSGE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002525-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="18667" checksum="B464EFA232C3F7FD" modified="2013-10-16" version="1">MPIKKKTDYVPIKCEGWDGKFVFAPGTTNNVSKVMRYNQNITNWNDFYGYCAEPPSPKVVWDGTFAKKRNRSDDSKFFAYNTEPEVLPPTWDGTFAKSGETYLKQERNQSDDSKYFSYNTEPEILPPTWDGSFKKSGETFIIPERNFSDARDYAEAKNFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGL6</accession>
    <name>T1GGL6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGL6"/>
    <dbReference type="STRING" id="36166.T1GGL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002539-RA">
        <property type="protein sequence ID" value="MESCA002539-PA"/>
        <property type="gene ID" value="MESCA002539"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_309795_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045189">
        <property type="entry name" value="UBR4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21725">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21725:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="754"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="754"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="780"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002539-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="951" mass="103268" checksum="5BA28C16DEDEE02E" modified="2013-10-16" version="1">MFLIASSGYIYYQPLDDESLAEHGDFYVTNTLELNHPLIKDLSGQIGGGGVSIYYSHMLQVLFFSYSLGRSFMAPLVNVNEGVKSVQQLQVNPVVSKNSSKGPPQPLCQWTEILGHPGLVCAMMHTSNNPVIFMIKPDTILTQEIKAQSSKAKIMDMVAIRHIVSGVEKTTLILLCEDGSLRIFTANVDVTNYWLSPEVLPIGNQINGVSKSSAKKNKKSSTAGKNQKSALSSSGQPVFPIDYFEHCTIMPDVEFGGNDLLQIYNTQKLKTRLMTTGTFVVCTKSTGFTLEVINSDPNMVIVGIRVQIGNQDPQRAPQSVTILGRTVMTPVRRARWFDIPLTREESLQSDKKLSIHFGRSHDVEDVTMLDSIEIYGKTKDVFGWPEDTEDVVGGTSSAQNTNTANQQSSSGTTDLQLQTVTSLDRMLTSMLEVLDSGLELLSGSNMDLDPQLRNKCIALSTILILNPCPNPVQNQAKCVLATLLGNRQSYHIYKDTMILKSVFDQLKELDAIESVQTIDPELFYRLVILTRSIALARSNSLAKICAENDYNILPLLLKLTKKLYEITPMLEEPTSVVRRGLSHVETTIHCLVEIIYAFALSDASKVEEMTNTKDAIIHLISPRLKRRKVVIVSPPACSTPTPPPSAVQDPEPPQAEAQPANNDFVPVEVDAIEPLGLEAVAAGGNNPFANLEAYLNAGRFPQLLDLQQDADDEAIMDIAIALSLQRHDADPQALQHGLASLANLQGIRNVAGSSANSNAAGSSGSGVEPSAGGSDDEASNAATDGSTLRTSPAEPAGSGGSESGGSGVESIGGASGRSSTYGDQPTQSPPRNNSGEVQQIAESVPSTSTAIPENIVSVDEVEQMETENLIKLHNLRCTILNKIIENLSFIDNVNGHQAIPLLQVAFQIELQLIHSQKFEFLMLPSQTRTTRACVQQLHLKGLCFQDLGFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGR8</accession>
    <name>T1GGR8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter family G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02007860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02007864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGR8"/>
    <dbReference type="STRING" id="36166.T1GGR8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002596-RA">
        <property type="protein sequence ID" value="MESCA002596-PA"/>
        <property type="gene ID" value="MESCA002596"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1742632_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF113">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY G MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002596-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16976" checksum="3F05D640D38BA639" modified="2013-10-16" version="1">MILLDEPTQYLNPLNAYLLISILSNISKKCGCGIVLSLEKPRSDVFPFLDKALFLCLGGVVYSGGTRTMLEYFHNIGFPCPQLENPLMYYLCLSTVDRRSRDRFVESSQQIDALIERFAREPIMSEINLPPKNVAKYPISYGKPTNSKFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH32</accession>
    <name>T1GH32_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH32"/>
    <dbReference type="STRING" id="36166.T1GH32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002716-RA">
        <property type="protein sequence ID" value="MESCA002716-PA"/>
        <property type="gene ID" value="MESCA002716"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270469_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002716-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11544" checksum="5ADD3E814021057D" modified="2013-10-16" version="1">SSYAGLHSLLKDGSKGAHSYYVSSIFYSAIFTSHPLNCVPTASVICPKFSEYLFERIGSADPDEWQDVSAACSFAVALMVFNMILYLFPLPKFVKKKFYKQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHB5</accession>
    <name>T1GHB5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02136489">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHB5"/>
    <dbReference type="STRING" id="36166.T1GHB5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002805-RA">
        <property type="protein sequence ID" value="MESCA002805-PA"/>
        <property type="gene ID" value="MESCA002805"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326667_0_0_1"/>
    <dbReference type="OMA" id="HFTEYEH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042982">
        <property type="entry name" value="GBX-1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334">
        <property type="entry name" value="HOMEOBOX PROTEIN GBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334:SF0">
        <property type="entry name" value="HOMEOBOX PROTEIN UNPLUGGED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002805-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11290" checksum="E3696D3C5A6FCDA6" modified="2013-10-16" version="1">MFENYDFFDYLEINLHFTEYEHSDSDGDCSEDGEGNMSSGVSGKGGALNAHSNSKSRRRRTAFTSEQLLELEREFHAKKYLSLTERSQIATTLKLSEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHU0</accession>
    <name>T1GHU0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHU0"/>
    <dbReference type="STRING" id="36166.T1GHU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003001-RA">
        <property type="protein sequence ID" value="MESCA003001-PA"/>
        <property type="gene ID" value="MESCA003001"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1580300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003001-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="18959" checksum="8CDD359B57ABF48E" modified="2013-10-16" version="1">MKIFFIGELCDFCLTPVKGSTNAFRRPCIGKSKCQGPGNSVIKNSPPNTSLPLVYFDMTANNQPIGRIVIELRSDVVPKKQKIFELCVHMKKDLVSGTHPSIASYQDFYVKEAISHMTMALVVNQFTESHLMTKTSFWSTADPVFCPWPALDQTQTYLSFISTFQKLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI00</accession>
    <name>T1GI00_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003066-RA">
        <property type="protein sequence ID" value="MESCA003066-PA"/>
        <property type="gene ID" value="MESCA003066"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072573_4_2_1"/>
    <dbReference type="OMA" id="FVSFMLN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03391">
        <property type="entry name" value="PAP2_containing_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="80"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003066-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="24933" checksum="02A9EF499C298DE3" modified="2013-10-16" version="1">MDPKTRLDDDDKRVVPPAIKKLLAWDVEMTKKFVSFMLNFVAFRSLKVHCKFLEYSCHGIVWLAGLMAYIWIVNSKASYQMQVNLMFGLLLDIVIVAIIKAATRRRRPTMNDEMFTIGMDKFSFPSGHASRAFFLLLFFTWLSPVSIIFWSPLFAWSFSVGLSRLLLYRHHILDVVAGAILGILEALLLCILWLGSDTCNWIMNTISEDYVPGGPEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIT3</accession>
    <name>T1GIT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIT3"/>
    <dbReference type="STRING" id="36166.T1GIT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003368-RA">
        <property type="protein sequence ID" value="MESCA003368-PA"/>
        <property type="gene ID" value="MESCA003368"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760732_0_0_1"/>
    <dbReference type="OMA" id="LMHNSAR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003368-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8254" checksum="FB5BDFE1915A7AE4" modified="2013-10-16" version="1">MAEVDEGLKRKRAFWKFTYRGVNFDQLVELMHNSARKQNLKRKPMALIKKLKKAKKEAPPMEKPSINNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIV5</accession>
    <name>T1GIV5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIV5"/>
    <dbReference type="STRING" id="36166.T1GIV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003390-RA">
        <property type="protein sequence ID" value="MESCA003390-PA"/>
        <property type="gene ID" value="MESCA003390"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_3_1_1"/>
    <dbReference type="OMA" id="FGCLFTF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000153">
        <property type="entry name" value="Endoplasmic reticulum chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000012">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003390-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="398" mass="44246" checksum="1C89C41AE2547FEF" modified="2013-10-16" version="1">KKKGKDVRKDNRAVQKLRREVEKAKRSLSGAHNVRLEIESFFEGDDFSETLTRSKFEELNMDLFRSTLKPVQKVLEDADMSKKDVDEIVLVGGSTRIPKVQQLVKEFFGGKEPSRGINPDEAVAYGAAVQAGVLSGEQDTDSLVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTAADNQHTKSQIFSTAADNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVSFEIDANGILQVSAEDKGTGNKEKIVITNDQNRLTPEDIERMVKDAELFADEDKKLKEKVEARNALEGLAYSTKNQLNDKEKWGGKITEEDKTKIEEACDEKIKWLEENPEADADTLKEKQKELEGVVNPIISKLYQQQGGAPPTGGEDMPEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ38</accession>
    <name>T1GJ38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02005844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ38"/>
    <dbReference type="STRING" id="36166.T1GJ38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003479-RA">
        <property type="protein sequence ID" value="MESCA003479-PA"/>
        <property type="gene ID" value="MESCA003479"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1181391_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051365">
        <property type="entry name" value="TOX_HMG-box_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45781">
        <property type="entry name" value="AGAP000281-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45781:SF1">
        <property type="entry name" value="HMG BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="80"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="80"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003479-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="235" mass="26070" checksum="3F1B743679BE81B4" modified="2013-10-16" version="1">MNIPTFHTPSFGDEDFDLVDEQEAEITPIPTSSFYGNSSFNTNDMIDYSPSGSTTSGDYHNNVVTSSSSSKPADSSTTEPVKPISPYASFFRDTVSSIQEQNPDISFQEISKIVNTMWEALPAVNKNIYKRQYEDEKREYVKAMNDFQNSQSQIDSKDNDEDDEEEEEENNVVTISPQVYNSDIPVEKVAPIHVSSSTPVTKTNLVTTNGDSTEDILSILSANICIRDGCGMTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ42</accession>
    <name>T1GJ42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ42"/>
    <dbReference type="STRING" id="36166.T1GJ42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003483-RA">
        <property type="protein sequence ID" value="MESCA003483-PA"/>
        <property type="gene ID" value="MESCA003483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_478670_0_0_1"/>
    <dbReference type="OMA" id="CASACMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000248">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1220.10">
        <property type="entry name" value="EGF-type module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF5">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="repeat" description="LDL-receptor class B" evidence="14">
        <location>
            <begin position="61"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="382"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="458"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="534"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="546"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="63972" checksum="7137D97B98A07D61" modified="2013-10-16" version="1">MRLDKENGKKCIPVDQVLLFVMENQIRGIDLQQPNHHIIPTISQSSQGLGPYNIDFIVAKNQIYWSDVFLNEVKTAKLDSGKIETVMNTDLIKPYGFAVDWIANNIYVSSGNQKCNIYATGLDGQFVTLIHKDLGSVKSIVLDPANGVMYWAEQELQSNRSKCNYDGTNRVILSNMVPKHPFSMAIYGDLIFWTDWVLHAVLRANKYTGSDVIVLRNDIERPMGVSAIQSITQNCASNECKILNGGCEDVCLLNKDGTASCKCSRGYLAKDGRRCLDRNKETNCTLDQFRCTNGECIPFFLTCDGLPHCSDESDEDIHYCAIRECPKNFFMCNNHRCIPFNETCDGETQCGDWSDEGDTVCKCEGEKFQCKSGQCIPNKYVCDGITHCKDGTDEWNCTHKECPTGDAQYIHCENTTGCYLPSSRCDGIADCLDKSDEEGCAHITSISCKTDQFRQYLCDGDEDCSDGSDERNCTCPSDSFMCANGKCIMNRWKCDGWNDCIDGSDENFETCENHKCHTNAPPPMRNCSESEFRCSNGKCIKSAHRCDGEYHCEDHTDEFNCNITCNANEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJC7</accession>
    <name>T1GJC7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003573-RA">
        <property type="protein sequence ID" value="MESCA003573-PA"/>
        <property type="gene ID" value="MESCA003573"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1637355_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003573-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18368" checksum="43C4DCFE6A1595AB" modified="2013-10-16" version="1">MKFADVVLEKSVSKKSTKSSRDIESDSDSDSSLDNYLVHPDKIDIATSFFERKENLPASTSKQSEEFNTGCLTQISTPKPESNIANFGHLIDHLQKVEDLKKKASDEVELKSRRPARYSLEDDIRETLAKMEAIPVPKSVKKKRKHGRDSEVESDWEEVEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJL3</accession>
    <name>T1GJL3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="9">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 9">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="9"/>
    <dbReference type="EMBL" id="CAQQ02187378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJL3"/>
    <dbReference type="STRING" id="36166.T1GJL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003662-RA">
        <property type="protein sequence ID" value="MESCA003662-PA"/>
        <property type="gene ID" value="MESCA003662"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_0_1_1"/>
    <dbReference type="OMA" id="ANASVMH"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF59">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="116"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="406" mass="44537" checksum="BC7FBE139671D4B7" modified="2013-10-16" version="1">MSGNSKLLHSKLAELDPELHGLIKKEKSRQRRGLEMIASENFTSLAVLECLGSCLHNKYSEGLPGQRYYGGNQFIDQIEILAQKRALEAYNLNPEEWGVNVQPYSGSPANFAVYTATSIFFESMPYKVNPTTGLIDYDALEANAKLFKPQVIIAGISCYSRYLDYKRFRQVADANDAYLFADMAHISGLVAAGIIPSPFEYADVVSTTTHKTLRGPRAGVIFFRKGVRKVKPNGDKVMYDIEGRINQAVFPGLQGGPHNHAIAGIAVAMQQAKTPEFVEYQKQVVLNAKRLSDGLISKGYNIVTGGTDVHLVLVDLRSAGVTGARAEYILEEVSIACNKNTVPGDKSALNPSGIRLGTPALTTRETSGPKLVDFKAAVDQNKDVLTLREQIENYSQNFPLPGFDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJV2</accession>
    <name>T1GJV2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02070025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJV2"/>
    <dbReference type="STRING" id="36166.T1GJV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003756-RA">
        <property type="protein sequence ID" value="MESCA003756-PA"/>
        <property type="gene ID" value="MESCA003756"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_17_8_1"/>
    <dbReference type="OMA" id="LTMEDTI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097254">
        <property type="term" value="P:renal tubular secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017085">
        <property type="term" value="P:response to insecticide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000479">
        <property type="entry name" value="Multidrug resistance protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF27">
        <property type="entry name" value="ATP-DEPENDENT TRANSLOCASE ABCB1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="693"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="737"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="791"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="903"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="945"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="43"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="388"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="697"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1001"/>
            <end position="1240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003756-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1245" mass="138582" checksum="409756F12814F4E1" modified="2013-10-16" version="1">MGSGQESESDSSKAPVEQKAPVKPVSFFSLFQYAKPLEYFWLFIGVIMSAIKALMLPAVVIIYSEFTAMLVDRTLMIGTTSTTYFLPILGGGKILVNATKAENNEELYKDSVSYGILLTIASLIMFISGVFSIDIFNLVALKIVTRIRIKAFYACMKKVLDGTTWFLTQTLLRRLQKKVAHFLNLIIGFVITVCISLAYGWKLTLAVSVYIPIVIVMNHYFGIYQSRLTTREQESYATAGNLAEEVLSSIRTVIAFGGEKAECSKFDKHLIPARIASKHKAMFASISDGILRSMLFLSCAGAFWYGVNLILANRFEEEKEYTPAILMIAFFGIIVGADNISRTSPFLQAFAMARGCATNIFKAIDEPSKIDPFSVDGKILNYGLRGVIEFRDVFFRYPSRPEKIIHRGLNFKVHEGETLALVGSSGCGKSSCVQLIQRFYDPVFGEIFNCWTRPVLFQGTIAENIRHGKPSATQREVEESAKAAGVHEFIANLPESYQTFIGERGVQLSGGQKQRIAIARALISNPKILLLDEATSALDYTSEKLVQEALDIASKGRTTIVVSHRLSAIRAADRIVYLDNGKVIESGTHDELMQLQGHYYKMVTAGDLKVQEDQSTVEEEMEEKFLKRKSLEIYEKSFELDTNNLDKNYKDFELPAMEKILNKEPKKKDDVEEEKTEFLKTFGRIFMMAKPEWVYLFFGALAAILFGFTYPGFALIFGEFYAALSETEEQVVLDKTANLSIACLIVGFITGFICFAQTYFFNLAGGWLTTRIRSSCYKTRLSGDAANVEGAIGYPLSGIIQALSNFIISTSFAFYYSWKLSLLCLSTCPIIIGTVVLEASLMSKQMLREKEALEKCSQISTEAITNIRTVAGLRREEYMIKKYNAEIRKVESLIRQKVRWRGVINSTGQAFMFFAYAVALCYGGVMVSRDEIPFQDIIKVSETLLYGSMMLAQSLSFAPAFTAALVAGHRIFKTIDRKSIIQSPTSHKGKALVKNEPFEGVRFSNIDFKYPTRPNIQILRGLNLDVLKGKTVGLVGHSGCGKSTCIQLLLRYYDPDSGKVFLDDDNIKTDLSLEAVRSKLGLVSQEPVLFERTIAENIAYGDNLRDVSLSEIMEAAKKANAHNFIINLPNGYDTKLGSRGTQLSGGEKQRIAIARALVRNPKVLLLDEATSALDLHSEKVVQDALDEACSGRTCIVIAHRLSTIQNADVICVVKNGIIAESGSHHQLMAKNGLYATLYKTQPKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK80</accession>
    <name>T1GK80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02156889">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003898-RA">
        <property type="protein sequence ID" value="MESCA003898-PA"/>
        <property type="gene ID" value="MESCA003898"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963443_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003898-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6612" checksum="EDD12E98885CCA41" modified="2013-10-16" version="1">MAEHSVEVETHIFQAIGSSLRPHCKFTMGSLSIKLNYYQLEVTKRFFPIASGTQPSIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKX5</accession>
    <name>T1GKX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKX5"/>
    <dbReference type="STRING" id="36166.T1GKX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004158-RA">
        <property type="protein sequence ID" value="MESCA004158-PA"/>
        <property type="gene ID" value="MESCA004158"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_13_0_1"/>
    <dbReference type="OMA" id="HDQASTN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032036">
        <property type="term" value="F:myosin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF49">
        <property type="entry name" value="FI08416P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="13"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004158-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17279" checksum="EF1CB293F733947B" modified="2013-10-16" version="1">MPMYDPPSKPKFSSVDEFQEAFNLFDNRGDGKIQLNQVGECLRALGQNPTESDVKKCTHQLKPDERISFEVFVPIYQAISKARSGDTADDFIEGLRHFDKDASGFISSAELRHLLTTLGEKLGDDEVEQLLSNQEDSQGNINYEEFVRMIMNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMN1</accession>
    <name>T1GMN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02165717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMN1"/>
    <dbReference type="STRING" id="36166.T1GMN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004807-RA">
        <property type="protein sequence ID" value="MESCA004807-PA"/>
        <property type="gene ID" value="MESCA004807"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019280_1_0_1"/>
    <dbReference type="OMA" id="YPWLDRS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000043">
        <property type="entry name" value="Scratch family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001484">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="168"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004807-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="509" mass="56732" checksum="78E3B849A11EB21A" modified="2013-10-16" version="1">MPRCLMAKKWKAYPWPDRSNSDESQEEDDEQQQQQQQQQTQKVIPTSIPTKTIVTNSQQKVKSQVTEDDEEIDVVGVGTSVIIANTTNVINVVGKQQEVGKSKLPTTSTCWGPSSPTEGTTAPSPPHIYRKQLLVIQLLYTTTIFYGSIHRLHKRLFHAFYSISTQDGERSNSNYIEPSSPHSSSSSPNPQYAYHHSPTPSTSSIASTSSSIDRYSPPVIHIKSEPNLSPTGRKVCYTSTGATLSLPPKKKDIYRPYSLDDRPSPKSERQYDNNALMRIPAEEDLHAAHAILDLSASTVLHPPQHPHQIHQHNLQMQQIASLQQHPSNTHQPQNQNPYGDNGSQSIEIVVPDELGLHHLHSHKSSSKKSPIVQNENSNSTINIVSPSSKECKTIAYTYEAFFVSDGRSKRKTPMETPEPKTKYTCTECGKQYATSSNLSRHKQTHRSLDSQSAKKCHVCGKAYVSMPALAMHVLTHKLSHSCGVCGKLFSRPWLLQGHLRSHTGEKPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMT1</accession>
    <name>T1GMT1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082009">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004863-RA">
        <property type="protein sequence ID" value="MESCA004863-PA"/>
        <property type="gene ID" value="MESCA004863"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925241_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004863-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6760" checksum="0DE3D9645C8EF3EE" modified="2013-10-16" version="1">MLQICLPVGQVLLLPSPDLIKKSGGEQHILLTPQDPQNIDFRRNLLTIGVHGLVHALKRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNT5</accession>
    <name>T1GNT5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNT5"/>
    <dbReference type="STRING" id="36166.T1GNT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005245-RA">
        <property type="protein sequence ID" value="MESCA005245-PA"/>
        <property type="gene ID" value="MESCA005245"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962113_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000001">
        <property type="entry name" value="GATA transcription factor (GATAd)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF337">
        <property type="entry name" value="GATA-BINDING FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="12">
        <location>
            <begin position="37"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14156" checksum="AB75D8DB233B41BF" modified="2013-10-16" version="1">MPPGVFSGSKPEGSIKLVVYRSMHSTVTCFHVGADLFTEGRECVNCGAISTPLWRRDNTGHYLCNACGLYHKMNGMNRPLVKQPRRLFCNSFLQVDMLIKKTDGADKAIKRSSTCLSLSSLPNNSNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNT6</accession>
    <name>T1GNT6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pickpocket protein 28</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNT6"/>
    <dbReference type="STRING" id="36166.T1GNT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005247-RA">
        <property type="protein sequence ID" value="MESCA005247-PA"/>
        <property type="gene ID" value="MESCA005247"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1125590_0_0_1"/>
    <dbReference type="OMA" id="NQANCEI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005247-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25745" checksum="F4CB5D0C288F9052" modified="2013-10-16" version="1">MSGVWEKKARGRQCYFPDEKYLRYFKVYNQANCEIECLTNFTLKSCGCVRYNMPRNNETLVCSSNMIECFNEAESKLLKEQFLEEVKYMKRSSNSNAGCKCFPLCTTITYEAETSESEDGYTFYEYMLRKQNNSLQNSFTSTAALFVSFEENQYIASKRSELYGTSDFLAAVGGLFGLFFGASMLSMVELFYYISLYIKGEASHLRKNSRNEPENERDLESGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPL0</accession>
    <name>T1GPL0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005543-RA">
        <property type="protein sequence ID" value="MESCA005543-PA"/>
        <property type="gene ID" value="MESCA005543"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029343_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005543-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="14087" checksum="67E7E3901258B55E" modified="2013-10-16" version="1">MTVIKRYLVLPPKQTSYDSHQRSTYDGPQATTWTSDPKYPLQPLENIESFFHKRTTELNRNIVLLLKGAIYESTRQSFPTKSSYTVLKSNLGPRPQRPMKAIIQQFGLDRLSLNKEEEIEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQM2</accession>
    <name>T1GQM2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02043376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQM2"/>
    <dbReference type="STRING" id="36166.T1GQM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005933-RA">
        <property type="protein sequence ID" value="MESCA005933-PA"/>
        <property type="gene ID" value="MESCA005933"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_3_1"/>
    <dbReference type="OMA" id="EHTFVDM"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00202"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005933-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="368" mass="41116" checksum="0624343E54BC35D7" modified="2013-10-16" version="1">MSFRSQPNGFASKAIHVGQEPEQWNSMCVAPAIITSTNYKQDAPGVHRGYYYSRGNNPTRNVLQKCLASLDNGKHGLVRTDVYGGINRLIRQVASRLGILYDFVDLTNFEALESAIRPSTKLFWIETPTNPLLKVIDIESVSKIAHTKANIVLVVDNTFLTPYFQRPLELGADIVFYSLTKYMNGHNDVIMGAAITNSDEIFEKLRFLQNATGIIASPFDCYQVNRSLKTLALRMEQHQKSAMKIAKYLEKHPFVEKVLYPGLPSHPQHGLALKQTYGCSGVFSFYIKGNLESSKRFFESLKFFTLAESLGGYESLAELPAIMTHASIPAEDRIKLGITDNLIRLSVGLEDVDDLIEDLEKALEASQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQW0</accession>
    <name>T1GQW0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQW0"/>
    <dbReference type="STRING" id="36166.T1GQW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006025-RA">
        <property type="protein sequence ID" value="MESCA006025-PA"/>
        <property type="gene ID" value="MESCA006025"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_642960_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006025-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="427" mass="47307" checksum="9FB2A0E1FE35B6A4" modified="2013-10-16" version="1">MTSNVSGRVAALTEKFNKIIEKDVNFLNEVKRNNGVLLSRAGHVYKIVEDKKQTTSTALVVTPPSPPQPPLQPPPQAPEQAMENNASPKHATVNNSVKETIKIFENLKAPTKVPPNRSTFKLDLILNSQELEKIDEVLTPVKDEGSLIAEDMHFDDETDKKILEAIDLVENKIAFLSKEHYDEEFKREEAEEVAVAKEVVEPVYAPLNPKPNESFLHSTKITSIPIIEAVNSSLIARKHIYDDENASNNGYSPIIYKEICNTYEYIKTAPTATDNDDAPETAEIIASACKDDIYEVCESKEKISQRTTTDELPPLPEPKRKTAPQEVARELPKIPSNCGAVDDLYDLGGEIEMEENIYATIRGYDGDNMSVISHNGYESIGGYQKIKKSPSISTISSDLKANSLYGVSMVPMVDGGESRTVLVKLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRM6</accession>
    <name>T1GRM6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-adenosylmethionine synthetase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRM6"/>
    <dbReference type="STRING" id="36166.T1GRM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006312-RA">
        <property type="protein sequence ID" value="MESCA006312-PA"/>
        <property type="gene ID" value="MESCA006312"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779551_0_0_1"/>
    <dbReference type="OMA" id="HINSCGE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="8">
        <location>
            <begin position="62"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006312-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="16430" checksum="D0D42E982CAA32CB" modified="2013-10-16" version="1">MNSVKEELGYGKSVVSLQHSENISLEDLCKEVSSKVIREVFLTQYFDENTIVHINSCGEFIIGRSKGDSLLTGRKIIVDTYGSWVAYGARAFSGQKFHENRSSVYAARLLAKSLLKAGIDKRCLGEKGERWVQQIRNELILQFKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS06</accession>
    <name>T1GS06_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vinculin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02086186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS06"/>
    <dbReference type="STRING" id="36166.T1GS06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006453-RA">
        <property type="protein sequence ID" value="MESCA006453-PA"/>
        <property type="gene ID" value="MESCA006453"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012338_0_0_1"/>
    <dbReference type="OMA" id="ANNLCEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.810">
        <property type="entry name" value="Vinculin, Vh2 four-helix bundle"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017997">
        <property type="entry name" value="Vinculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000633">
        <property type="entry name" value="Vinculin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46180">
        <property type="entry name" value="VINCULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00806">
        <property type="entry name" value="VINCULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00664">
        <property type="entry name" value="VINCULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="673"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="386"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="884" mass="98060" checksum="775EFDED76A41BA8" modified="2013-10-16" version="1">MPDLSQPVQAVSLAVSNLVNVGRGTINSSTDEILKQDMPAALNRVENSAQLLVEATSMLKNAPSSAQARKKLIDGSRGILQGTSALLCCLDESMVRKIIQECKRILHYLTLTEIMENENDLEKLITVISKSDFVQQIEARRLELTHQVHSDILGRCIKEFQNLLPVLFDSMKLYILLALEQQAPNMPTPHPDAIATAADNRNYLATRMSDEVQEIIRVLQLTTYDEDTSELDNITVLKKISLSGGFGEKALRQIIDDVHRVADYCLPQDAIPIRKMADDITAMANALCELRQEGKGNTPQAEALAREIRAKLANLNDAVLQAVVNLDKSGVPQNAHTIKGRLEQACKWLQNPNINDKGLGQRAIGLIVNEGRKVADGCPGHQKAEIQQLCNEVENLSDKYSNLCARGLGNTPEAQATARLLNEKLHELKSKIQNALVNRVVEDFMDVSTPLEQFIEAVNEGIRVKPFNEENFNHKANNLTNFSDRAAKTSRMVAASASGGNKRIAEALLASAAHIDSLTPQLISAGKIRVKCPWMGPAEDHLLNLKKQYADTILRMRNAIGQKSPQKMVDNTSNIARLVNRVLWVAKMEVHNSENPEFTAKIKSATAKLEEALPQMVQCAKGVALNINDPNAAHAWRESNKKLLNAVRNVGNAVVHTPEVPQPPDMSALRLNDYEHAPPRPPLPLEPEFVPSRPPPPAETDDDEDAFRAMPHANQPILIAARGLHQEVRQWESEDNEIIKAAKRMAKLMARLSELVNLKGSKSELIATAKAIAEASEDVTRIAKDLARRCTDIRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQGSEEDREATDMLVGNAQNLMKSVTETVRVAEGASIKIRYDQTHYRMRWVRRQPWYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT02</accession>
    <name>T1GT02_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NudC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GT02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006818-RA">
        <property type="protein sequence ID" value="MESCA006818-PA"/>
        <property type="gene ID" value="MESCA006818"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1403925_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025934">
        <property type="entry name" value="NudC_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14050">
        <property type="entry name" value="Nudc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NudC N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006818-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="21933" checksum="28F567ACB66E17D7" modified="2013-10-16" version="1">MGESLKMSLQEKHDNLFMEIFKAYQNPADFLDEVFGFLNRKTSFFDIYDEQRKVGLPPGTXXXXXXXXXXKKRKVGFPPGTATKIINDAMTKFQKVGIQNPKEFTSENENLKTMSMSGPELQITESKSDIKRTFSDSEHFNGAINDFYCWGQTEDDFEINVFLPNHISSTNDFKINPPPLVAVPGGNPTFLCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTA9</accession>
    <name>T1GTA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTA9"/>
    <dbReference type="STRING" id="36166.T1GTA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006935-RA">
        <property type="protein sequence ID" value="MESCA006935-PA"/>
        <property type="gene ID" value="MESCA006935"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="OMA" id="IQQKNNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006935-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="22122" checksum="D487EAE306AB1041" modified="2013-10-16" version="1">MRRQWSWEEALLNQYVNQTFYESFNSTIGVDFYLKNLKINKENIKLQIWDTAGQERFNAVCSAFYRGADCCVLVYDVTDRVSFENLELWRAKFISAANVALNDDFPFVLIGNKSDLQDREVSSELAKNWADFDDIPFFETSAKTAVDVDKAFLKVAQLVEKRQPWEVVHPIEYNEDTRPQSSTREKSNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTV5</accession>
    <name>T1GTV5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02060123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02060124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTV5"/>
    <dbReference type="STRING" id="36166.T1GTV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007149-RA">
        <property type="protein sequence ID" value="MESCA007149-PA"/>
        <property type="gene ID" value="MESCA007149"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944327_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007149-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="7055" checksum="2E6160E1685B0E12" modified="2013-10-16" version="1">MSKVKKLTTIYSRNIEYKITTNQCFKFNSRKFTFMEGSDICFEVVRRALAGFVYSEAVAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU02</accession>
    <name>T1GU02_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02037815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU02"/>
    <dbReference type="STRING" id="36166.T1GU02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007201-RA">
        <property type="protein sequence ID" value="MESCA007201-PA"/>
        <property type="gene ID" value="MESCA007201"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_0_1"/>
    <dbReference type="OMA" id="LLWFIWM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="74"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007201-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="442" mass="48802" checksum="A4A22DE4F75D3902" modified="2013-10-16" version="1">MGVRRAGYSRGYEEMTGSNVPNSNSTPDLSNNGNITPTLGPEFELSSVTVIPDDTFTVQQAVNALGFGWFQVKLSLCVGLCWMADSMEMTILSVIGPSLHCDWGITRYQQALTTTIVFMGMMLSSTFWAQLSDRYGRKPALTLCGILLFLYGLLSAVAPSFGWLLFLRGLVGFAIGCVPQSVTLYAEFLPAKNRAKCVVLLDCFWALGACFEVVLALAVANLGWRWLLGLSAAPLFIFSLITPWLPESARYHVSNGNTEKALQTLEGIAKDNKRPMLLGRLVVDGTTPIKGSIKSLLNPGLRRTTILLWFIWMACSFCYYGLVLMSTELFSSEVTENNTDCRPLLQTDYMDLLWTTLAEFPGIFATIAIIERFGRKSTMALQFMLFAICVMILPSTETRIALTLTLFFARGIIAGLFQAAYVYTPEVYPTALRSIGLEVVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUG4</accession>
    <name>T1GUG4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007377-RA">
        <property type="protein sequence ID" value="MESCA007377-PA"/>
        <property type="gene ID" value="MESCA007377"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742955_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007377-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8460" checksum="FD2F702974EEA3A3" modified="2013-10-16" version="1">MYASQHVDRLVLDFSNFPQIGKLPPKPNPLIPSKRRCKKLRKFLAWRGDLYLFLENAQIDQNRLKVFTKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUJ5</accession>
    <name>T1GUJ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007409-RA">
        <property type="protein sequence ID" value="MESCA007409-PA"/>
        <property type="gene ID" value="MESCA007409"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963411_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007409-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6644" checksum="2D12696D935781ED" modified="2013-10-16" version="1">MKGIHGNRHGHEKDNEIKILTDGTDGIPVEMLKTAGASFNRVLPYYSEEVPNEWNFSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUM1</accession>
    <name>T1GUM1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ApaG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GUM1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007438-RA">
        <property type="protein sequence ID" value="MESCA007438-PA"/>
        <property type="gene ID" value="MESCA007438"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051858_1_0_1"/>
    <dbReference type="OMA" id="IMPYSST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="entry name" value="ApaG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011722">
        <property type="entry name" value="Hemimethylated_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14289">
        <property type="entry name" value="F-BOX ONLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14289:SF16">
        <property type="entry name" value="POLYMERASE DELTA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="ApaG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApaG" evidence="2">
        <location>
            <begin position="152"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007438-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23804" checksum="3E1F6D7FECB64B5B" modified="2013-10-16" version="1">VYDRDISANHEKSRRNIKDAEKSTSAETKEKDKEPPNASSASKEIKAKLNTFYQVLIDSRDCPFIRAQTEAVTFLGNQDSNRSLYAIPGLDYVSHEDVMPYTSGEKSPVHHELFDKFLAHVPDKDPSFEGQPTLKAWHQKNHPWLELSDVHRETTENIRVTVIPFYMGCRDTPASSVYWWRYCVRLENLGELSVQLRERHWRXLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUM6</accession>
    <name>T1GUM6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUM6"/>
    <dbReference type="STRING" id="36166.T1GUM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007443-RA">
        <property type="protein sequence ID" value="MESCA007443-PA"/>
        <property type="gene ID" value="MESCA007443"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888145_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055577">
        <property type="entry name" value="DUF7153"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22198">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22198:SF1">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23672">
        <property type="entry name" value="DUF7153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7153" evidence="1">
        <location>
            <begin position="45"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007443-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15549" checksum="A9C156FE886DB3B3" modified="2013-10-16" version="1">MSSEDLNIVKICNMAKFQKTKTTVDPRIEFPRLFGALFSLGVVKNVFEGDDGERFEKNWLYWTGARMMYRYLPRTVGLRRISLHKSVSQRGDKMYLLICECSELLKELSSAAVLIPALRARLCGYTGLYRPIQAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUX2</accession>
    <name>T1GUX2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146457">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUX2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007544-RA">
        <property type="protein sequence ID" value="MESCA007544-PA"/>
        <property type="gene ID" value="MESCA007544"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2336004_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007544-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11531" checksum="03F0512B6EA0CC6A" modified="2013-10-16" version="1">MDGQILKCPLINFKIGFDFDIIDGQITLKKLFLRNTQIHLSLNSLFTYDTPMRDELYPAINHFGNKEKLIRLCQLKLRKTWSCVRAALSYSFLTSCWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV57</accession>
    <name>T1GV57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mediator complex subunit 28</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV57"/>
    <dbReference type="STRING" id="36166.T1GV57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007641-RA">
        <property type="protein sequence ID" value="MESCA007641-PA"/>
        <property type="gene ID" value="MESCA007641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088358_0_0_1"/>
    <dbReference type="OMA" id="NIGMPER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021640">
        <property type="entry name" value="Mediator_Med28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13512">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13512:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11594">
        <property type="entry name" value="Med28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="28692" checksum="3D351A1A1B88DC71" modified="2013-10-16" version="1">MASSTNGNGNLMDEFEEAFQACLHALTKKETNTGVTKDEIELEVHETTNKFIDIAHQMEVFFLQKRFLLSTKPEMLLHEENTDLKNEIVRKEGLIKKHYNNLDEWKKLLNQQQSHHSSQQMQMQQQQQIPGGEMWQGGAGGMNKPPGIPPNMMSPQMAGVGGMPINPMHQQNPQHYQQMQQQQMLHAQQQQQMFQMQQQQNFIGPNRSPASGFVGQPNSNRMPSPLTYLEKTTSNIDMGNMGDRSLSILVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVD0</accession>
    <name>T1GVD0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02026256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02026257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVD0"/>
    <dbReference type="STRING" id="36166.T1GVD0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007724-RA">
        <property type="protein sequence ID" value="MESCA007724-PA"/>
        <property type="gene ID" value="MESCA007724"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1322253_0_0_1"/>
    <dbReference type="OMA" id="IMHESIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007724-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="22877" checksum="A781BFCAF19A4F4B" modified="2013-10-16" version="1">MILNEVLSEIKLNSEPLPVATVKLFNIKCNQGIGFSRLLIIRELYNNLYKVTSVLNKCFGLSILVSVGNDFLSITSNFYWIFLTFQVSSNSTQDFLRIAGSAVWCIPHLFTVVLLSYLCHLTTQNASLISLNLHKIDIDVGNIHHNAIIAGATTTYLIILIQFHMSEQSGSANTVTSIATSVATSMATSPPTTTIPTITLQIFDNSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVH2</accession>
    <name>T1GVH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080632">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007776-RA">
        <property type="protein sequence ID" value="MESCA007776-PA"/>
        <property type="gene ID" value="MESCA007776"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152070_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007776-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12626" checksum="34F473F4E6362D21" modified="2013-10-16" version="1">MNQIFTTSFLTSPPPSDVDFPGMKPHWYGPTMCSTCDFRCSVTMVSPPPPPPPPPPPPPPPPPQKHGVKMPLSRMLWYPFLTATTSRMLYAILKIIYHIIPGFLIDTYLRMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWN6</accession>
    <name>T1GWN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongation factor EFG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWN6"/>
    <dbReference type="STRING" id="36166.T1GWN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008214-RA">
        <property type="protein sequence ID" value="MESCA008214-PA"/>
        <property type="gene ID" value="MESCA008214"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_9_0_1"/>
    <dbReference type="OMA" id="QNTVEHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000005">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor EFG" evidence="5">
        <location>
            <begin position="299"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008214-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="396" mass="43031" checksum="48D94643DF297666" modified="2013-10-16" version="1">PAPSERNEIYNCFGNDFVGKVFKIVHDKQRGPLTLVRVFRGDIKKGMRIMTSSGVAETLQKIYEPLADEYREIGEVCAGGVAVCSGLKNTITGDLLTSSTSSLKSAESKIAKESEDESNVLSLKPYIPDAVYFCSIEPPSLSFQTPLEIALKQLQREDPSLRVVFDETTGQTVLGGMGELHMEVIKSRILTEYKIDADLGPLQIAYKETIESELEETFVVEKEIAGTKQSFENDSSESFANVKKGALGALERGPRVGGNVIGVQVKLHKVTIGRGTADSFLMSAAGQCVQKILNDIGTKLLEPIMDLQIIVPSERVVSAVLGDLSRRRATINDVLPKGEQTKLILVHAPLAELSGYSSTLRTISSGTASMTMQPFGFQGMNSNDELVAIRRAQGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWV0</accession>
    <name>T1GWV0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWV0"/>
    <dbReference type="STRING" id="36166.T1GWV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008282-RA">
        <property type="protein sequence ID" value="MESCA008282-PA"/>
        <property type="gene ID" value="MESCA008282"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_633548_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008282-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="433" mass="49071" checksum="1BBF67636013478C" modified="2013-10-16" version="1">MSRQQQVKIQNVAKSKSSNGLPTFLRWFKKSKETVNPSGALSSASQISSSQDSLGDLENRNNFTTSSESLVSTATNGFAFIQPKNYEQTNGIGIKTTNIENHPVEESSSLLRRIRLHNTRRNHQINCRISLKEKYCLQESDTLPSNKHRIPEGIECTYENLDPNRRHTADNKNFCRNKRNNFRRTVSDSSKDISSGAYSHVKGKRRAPAPPKIEMPVQVEDQLERKSSVSVESTLSRKKRRAPPPPNLTPTSNDISDESSKQAPTSPPEVAKCSPRPWYKRSVAINKNSDIPFKREINLKTMEKRKKKDFNKKMEKEPLPDCANSRVSIFDANNKLSILLRNKQEEKDRRKSGIAMPNISELDKETDNFIKTRESLTLTNDIILQQKMFEQASSSTEQKPVQESTEPKSSKDLIAKFESQPSTSKSDDLDSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX28</accession>
    <name>T1GX28_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">kynurenine--oxoglutarate transaminase</fullName>
            <ecNumber evidence="2">2.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-kynurenine degradation; kynurenate from L-kynurenine: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.7" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02083727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX28"/>
    <dbReference type="STRING" id="36166.T1GX28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008366-RA">
        <property type="protein sequence ID" value="MESCA008366-PA"/>
        <property type="gene ID" value="MESCA008366"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_6_1"/>
    <dbReference type="OMA" id="HYMEAVA"/>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00726"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000021">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="17"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008366-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="21799" checksum="709806D84CC0AD4E" modified="2013-10-16" version="1">MLVGASRIRAPNAAVRKTFSLTGWKTGWAYGPKELMTNLQMVHQNCVYTCSTPIQEAIAIGFEEELAKLDSPECYFNSLPKELKEKRDFMYKFLKEAGMKPTLPEGGYFMLADWTSLENKVDLNSESDKYRDYRFTKWMTKNQGLQGIPPSAFYSVPNKNLGENFVRYCFMKKDENLTKAAEILKNWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX43</accession>
    <name>T1GX43_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008383-RA">
        <property type="protein sequence ID" value="MESCA008383-PA"/>
        <property type="gene ID" value="MESCA008383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592483_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8971" checksum="67766171BA926F98" modified="2013-10-16" version="1">MNNIPHLGKFEQIQSKGYNQKVFCKAGSRKFYIAVVLLQGGGPADVFSSHDLTPKMLEDLRLSESTFLVTRDFISSHAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXI6</accession>
    <name>T1GXI6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008538-RA">
        <property type="protein sequence ID" value="MESCA008538-PA"/>
        <property type="gene ID" value="MESCA008538"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1789065_0_0_1"/>
    <dbReference type="OMA" id="NAHAVNH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577226" evidence="1">
        <location>
            <begin position="20"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008538-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="14476" checksum="7F55B6B0F9E53B35" modified="2013-10-16" version="1">MMTKCMIFAVLALCACVNGGIIPAGVVAPYASTYNAHHINHAVAHPVVAAAPAPVHFAAPAIAAAPAIAAPAPVHFAAPAVAAPAIAAPAPVHFAAPAVAAAPAHFAAPAPFVAPSTSIHMLPQLWLLPHSSMDSQDTSRSLILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ0</accession>
    <name>T1GXJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DEUBAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADRM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXJ0"/>
    <dbReference type="STRING" id="36166.T1GXJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008542-RA">
        <property type="protein sequence ID" value="MESCA008542-PA"/>
        <property type="gene ID" value="MESCA008542"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369281_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEUBAD" evidence="5">
        <location>
            <begin position="52"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="82"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008542-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="20452" checksum="1F0EB151EC8CF3C0" modified="2013-10-16" version="1">RDGSSRSARTGTPSRSSAAASTAATPSTPENTTTAVPKVPAVMPSALSPAASHGNRGNIDLTSAITSSSDAVNKVISDPERVQKLASHLPDLGEAAPSTSNSPTEVKQQVKDTITSPQFQQAMSLFSNALQSGQLGPVVQQFEVTSDAVAAATTGDMEQFVKALEKKGSSTGSTSSGKESVETMEEDEPAASSAATEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY43</accession>
    <name>T1GY43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II elongation factor ELL N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GY43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008763-RA">
        <property type="protein sequence ID" value="MESCA008763-PA"/>
        <property type="gene ID" value="MESCA008763"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_543252_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF17">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase II elongation factor ELL N-terminal" evidence="2">
        <location>
            <begin position="27"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008763-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="502" mass="54564" checksum="5AF0C0CA4081E9A3" modified="2013-10-16" version="1">MASLCTGTNYGLSQLKNNNRGGATNNNNNSSGGGKEYIFVKMTDSAFQAVEEYQRQLAKNSNCGAATIQFMGNSGMLAFPVDNVKSSKFRFSVDLVDDSSIECIEQTRNHLDVVGAMSNRIRIQANDDVYEKTRNRMATIEENQKTKCAREIKPNQTDIGRKVKVKTGNNVMLNSISNSNSAIGSSQQFNINNYRSTSSNSSSLSXMLNSISNSNSAIGSSQQFNINNYRSTSSNSSSLSNQSAAAAAAAANSVNYKNGNSRTSTVPRTSLPPSSMTTNNHNNNTTASHQLQHQQQQQNHHLKNNLSANSVNSNNSTTTPISSTANSNNSNAPKSEISKRKIRERLIHLLALKPFKKPELIQRLNNEGLRDKERGLIRNILNEISQMKDNCYNLKHAIWNEVNENWPFYTEQEIQQLKRRKPQNLTPESSDGGSSSISGQSPSSAHNGCSPSMMGVKRPPSSSVGAINGGGSSGNGVSAGGVITDNSYDDISMMQGNRTILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY96</accession>
    <name>T1GY96_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008819-RA">
        <property type="protein sequence ID" value="MESCA008819-PA"/>
        <property type="gene ID" value="MESCA008819"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064134_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008819-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="14118" checksum="4CCA7AB0D5F1B500" modified="2013-10-16" version="1">MYYVLSVHTNVISGLENQHSNKLSHSGRPLRRPKKTISIRSIFLANRCLFNLNRLLLCNLYFQCFCTDLTVGSSTIKFVSYFERKHHFNHNHELYRLYGYPRIFSLIRTKLIRWSGHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYB1</accession>
    <name>T1GYB1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYB1"/>
    <dbReference type="STRING" id="36166.T1GYB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008835-RA">
        <property type="protein sequence ID" value="MESCA008835-PA"/>
        <property type="gene ID" value="MESCA008835"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075398_0_0_1"/>
    <dbReference type="OMA" id="MNIVIGY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF168">
        <property type="entry name" value="GH22922P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008835-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="251" mass="28721" checksum="202947A9C2712A11" modified="2013-10-16" version="1">MPSSLDIFAKTSKLQLIDLSGNDIEEPMLKRIHFKSLHALSSLDLSRNTNINGIDYLAFKFTALKALNMSSVRLMNLNKLGNDNITNIDFSNNEISQIPVNSFEDLKNLITLNLSCNNILELKEPVFRDTKNLKELRLQGNNLRKLDFKIFQNLVNLETLDLSSNCLESIPELMNLIKLQNLFLQDNFLKIIPSNRALPITLKTLDISNNSLRFIGKNTFQNIQSLNSLDISYTNNTYIDFDTNVYNPFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYL9</accession>
    <name>T1GYL9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NIF system FeS cluster assembly NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYL9"/>
    <dbReference type="STRING" id="36166.T1GYL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008952-RA">
        <property type="protein sequence ID" value="MESCA008952-PA"/>
        <property type="gene ID" value="MESCA008952"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_5_0_1"/>
    <dbReference type="OMA" id="SMVTEMV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000001">
        <property type="entry name" value="Iron-sulfur cluster assembly scaffold protein IscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008952-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="13008" checksum="B4B8907D9AB26EFF" modified="2013-10-16" version="1">VVDHYENPRNVGSFDKKDKNVGTGLVGAPACGDVMKLQIRVDDNGKIIDAKFKTFGCGSAIASSSLATEWVKGKSLDDAGKIKNSDIAKELCLPPVKLHCSMLAEDAIKAALEDYKIKQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYQ7</accession>
    <name>T1GYQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008994-RA">
        <property type="protein sequence ID" value="MESCA008994-PA"/>
        <property type="gene ID" value="MESCA008994"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515213_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008994-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="10056" checksum="599BE9D96ECEC642" modified="2013-10-16" version="1">MFKSKTFSTYIFYHTIPTFKVNLVIGESILRLISFTESRNTRACSPRKRKLIMERMERLDSILPSFKLMVRWNGVYCLQTDLCLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYS8</accession>
    <name>T1GYS8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009015-RA">
        <property type="protein sequence ID" value="MESCA009015-PA"/>
        <property type="gene ID" value="MESCA009015"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815346_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009015-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7833" checksum="503316E9CA6BC772" modified="2013-10-16" version="1">MICMQYVPNIGVLYSNNKTYPNLRVLVLLNVRPKFSLPTVSLFKEISSGHSINVHQIRHKQKDILYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZG4</accession>
    <name>T1GZG4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009264-RA">
        <property type="protein sequence ID" value="MESCA009264-PA"/>
        <property type="gene ID" value="MESCA009264"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429595_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10367" checksum="BC734B744E4E9FBB" modified="2013-10-16" version="1">MDTWVYSGRSSDLSQGWPLHVLYMMLEMLKSQTHSFIQPYNGIQDKIPFLMIPVKNLGAKFILQILAIFDSRTDTSLLNDKVVDHLGINGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZL7</accession>
    <name>T1GZL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384282">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009321-RA">
        <property type="protein sequence ID" value="MESCA009321-PA"/>
        <property type="gene ID" value="MESCA009321"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725066_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009321-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8676" checksum="A0027C3ED2E1646C" modified="2013-10-16" version="1">MNGKMIPMMMTSLIEPFCKRNSGWQKLKQKRSFQMKIFKENWTRSLKKSKTLTKKLQNITKKLRNSKQIKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZM7</accession>
    <name>T1GZM7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02117187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZM7"/>
    <dbReference type="STRING" id="36166.T1GZM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009331-RA">
        <property type="protein sequence ID" value="MESCA009331-PA"/>
        <property type="gene ID" value="MESCA009331"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172064_0_0_1"/>
    <dbReference type="OMA" id="DTNGSMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="7">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="5">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009331-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9019" checksum="8E021AE89368068D" modified="2013-10-16" version="1">SDGIVGTGTITSPWTPVSAGPPGPLGPADTNGSMADSKNLDVGDMSDDEKDLSSADAEGVWSPDIEQSFQEALQIYPPCGRRKIILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZQ3</accession>
    <name>T1GZQ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DEPDC5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZQ3"/>
    <dbReference type="STRING" id="36166.T1GZQ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009357-RA">
        <property type="protein sequence ID" value="MESCA009357-PA"/>
        <property type="gene ID" value="MESCA009357"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029150_0_0_1"/>
    <dbReference type="OMA" id="TSHEWER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of TORC1 signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045838">
        <property type="entry name" value="DEPDC5_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19418">
        <property type="entry name" value="DEPDC5_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEPDC5 C-terminal" evidence="1">
        <location>
            <begin position="49"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009357-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="339" mass="39855" checksum="DC9FD40E5B5EFBFC" modified="2013-10-16" version="1">MLVSFLLDSMERLICHASGDWEKPIIPGFYLYYIVTQDKKSKDYLPPLGDLRAFENEWMEVEIQFGNYFEMSTPKVSTVPNFLRETPLRDNYWYSVNRQMYRSVHLEIDANKKSDRMEWGHAKYHSVMVPNTAFEIVVDWVTASGPAVSDLINGWSRKASQYNFQLQPIPADPLAEPFTEKSDPLRGPIFIPLNTSCLLKDKECLFNEFHEETRLKGCYCFRKLFWCPKEYQYVHCSGNMFVLIRCPQSSFPTTGSPLRHEVNVSRMVYGHTFNTNVPKKVGFLWSWNHMIPNKKWKSFLIGGTQELFQLKMLKDFREFCSNQDDRLLGFWNESYKCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H023</accession>
    <name>T1H023_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02004951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H023"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009487-RA">
        <property type="protein sequence ID" value="MESCA009487-PA"/>
        <property type="gene ID" value="MESCA009487"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF54">
        <property type="entry name" value="CYP9F3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21719" checksum="8553D6A81B44FF06" modified="2013-10-16" version="1">MSVLEKCNGKLEYENIKDLXXXXXXXXXXXXXXXYENIKDLVYMDMVFNETLRMYPPVPIYMRKVLNDYKIENTDITLEGGRGLMIPVYAIQHDSEIYPEPELFNPERFTPEEVSKRHPLSFIPFGEGPRICIGLRFAKMQAYVGLVELLSKFKFVPSEKTPQKIEFIKDKFFLTAEGGIPLKMELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0B4</accession>
    <name>T1H0B4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0B4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009588-RA">
        <property type="protein sequence ID" value="MESCA009588-PA"/>
        <property type="gene ID" value="MESCA009588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576574_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9537" checksum="0BC8A824C80D44B5" modified="2013-10-16" version="1">MPNINLTTLRLPVNNIHEDSVRTAMSGSRYHVELNVERPLLLEYPDHWLFSSPRIAHKKFLRVVSPTEKKEYSQFFCIGHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0G6</accession>
    <name>T1H0G6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0G6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009644-RA">
        <property type="protein sequence ID" value="MESCA009644-PA"/>
        <property type="gene ID" value="MESCA009644"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1688736_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009644-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="18426" checksum="645BEDAA27CCF7EA" modified="2013-10-16" version="1">MLQSALRVVRVWTKIKGLVAKYLGVILDRWLSWEENIEERRKTSFIAWFACINCIGKSLGFSPMIIRWLYLSIKRPILTYAYLVWCMPYVKRFVVLDWREIKGSMCWHDELSKVNSYHLSPMDLVVESIVTKSALRLQRVGLFKERSYGHKAILPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0U9</accession>
    <name>T1H0U9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02139261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0U9"/>
    <dbReference type="STRING" id="36166.T1H0U9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009791-RA">
        <property type="protein sequence ID" value="MESCA009791-PA"/>
        <property type="gene ID" value="MESCA009791"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010647_2_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000446">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="182"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009791-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="307" mass="33946" checksum="5C0549AC4AE89C72" modified="2013-10-16" version="1">MDDLKKISDLRTPANWYPNARTITRKIIFHAGPTNSGKTYHAMQRFLTAKSGVYCGPLKLLATEVFYKANEKGTPCDLITGEERKFGISEDKPASHVACTVEMTSVNTPYEVAVIDEIQLLKDPQRGWAWTRAFLGLIADEVHVCGEAGSLELLQKICETTNETVELFNYNRLTNLTIEDSALVSLDNVLPGDCIVCFSKNDIYNVSREIEARGKEVAVIYGGLPPGTKLAQAAKFNDPDNSCKIMVATDAIGMGLNLSIRRIIFYSIIKPTMNEKGEKEVDTISVSNALQIAGRAGRYKTQWENGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H106</accession>
    <name>T1H106_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02375906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H106"/>
    <dbReference type="STRING" id="36166.T1H106"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009857-RA">
        <property type="protein sequence ID" value="MESCA009857-PA"/>
        <property type="gene ID" value="MESCA009857"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_1_7_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009857-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="19378" checksum="A5CF864D91224E75" modified="2013-10-16" version="1">SRDDTGISRGEWPWLAAIYVNNLTSLSYQCGGTLISSKIVISSAHCFHMYKKQYTANEVLVFLGRHNLKNWNEDGSVAAPCDDIFIHPDYDSNLKSYDADIAVIVLKNDVRFNMFIRPACMWSGSTSLDFIVGERGKIIGWGHKTNQFQLRDDCDLYYVCLTEEGHGKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1D9</accession>
    <name>T1H1D9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Interacts with CCDC32; the interaction is direct and mediates association of CCDC32 with adaptor protein complex 2 (AP-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02381001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1D9"/>
    <dbReference type="SMR" id="T1H1D9"/>
    <dbReference type="STRING" id="36166.T1H1D9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009998-RA">
        <property type="protein sequence ID" value="MESCA009998-PA"/>
        <property type="gene ID" value="MESCA009998"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061221_3_1_1"/>
    <dbReference type="OMA" id="QSNFVEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000004">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="12">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009998-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16964" checksum="CEC12B1920377942" modified="2013-10-16" version="1">MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYSVVDEMFLAGEIRETSQTKVLKQLLTLNSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Q9</accession>
    <name>T1H1Q9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-citrate synthase/succinyl-CoA ligase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
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            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="8"/>
            <end position="94"/>
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            <begin position="107"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010126-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="20223" checksum="B1AF0DF595A3B258" modified="2013-10-16" version="1">MDGNIGCLVNGAGLAMATMDIIKLNGGSPANFLDVGGSVKEDQVLKAFQILTSDPNVKGILVNVFGGIVNCATIAKGVVAASKTLELKVPLVNNLNYVAMDGNIGCLVNGAGLAMATMDIIKLNGGSPANFLDVGGSVKEDQVLKAFQILTSDPNVKGILVNVFGGIVNCATIARGVVAASKTLELKVPLVVRLEGTN</sequence>
</entry>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA010329"/>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10313" checksum="59F02FAD56730F20" modified="2013-10-16" version="1">MVESPLKNFLQNSITTKKILDQQELKWTKNRKVHLLPHLPKSAPEQRDVCLSTRARDSKQQKLQILIRNSINSLKNSGVEKSCLSNETST</sequence>
</entry>
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    <accession>T1H2H1</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        </location>
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            <end position="32"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="14965" checksum="444093182A881B3D" modified="2013-10-16" version="1">MGIENPAVITTDCEGRVNENPNTEDEERDEFDGEKHSGCFPESPFSLLGESEFDEDLGFPDSVTILSVGGNNPVLVQKKDDSADEAESTGELPITALYNPALKFNSLPLRLFCTNPVLLDTSSKPQKIAHYQLEYI</sequence>
</entry>
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    <name>T1H2N4_MEGSC</name>
    <protein>
        <submittedName>
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</entry>
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        <submittedName>
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                <person name="Hughes D."/>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="375" mass="43103" checksum="2EC332F3096D2B90" modified="2013-10-16" version="1">MVPITGNKQEENVVISTFEIDEIPTQLKQTQTLPNVSDMFVLTDDPENPVAFNNITEDEIMHNTPDNIILHNIDTISAEQPQSADISSNPLVTIKDVSSNPYVAIKAEVLSAVDNLPRRPKPIQSSSPSYLSARLDNSELAPVPNPPEEDDEIPLWKKWYDVLKNCPPREYVSPPKSPRKKRPRKKITTQIETERSMSPVFLPPEERNRTVIPSQRTIQRSMSPVFLPPEERNRAVIPSQRPIQGASESMSNVIQHPDKVNVHHEEMISSFNNQFKKFEAKWKKKIEAGEKSPNWWNRREMFFAGIVQQIEDVKNNEIYAVEKKNNLHLKDGESILYLLKELKIKKEIRISELLEKNEATFNPIINGKLYINIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Z4</accession>
    <name>T1H2Z4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02145636">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02145637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Z4"/>
    <dbReference type="STRING" id="36166.T1H2Z4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010601-RA">
        <property type="protein sequence ID" value="MESCA010601-PA"/>
        <property type="gene ID" value="MESCA010601"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444390_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010601-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21290" checksum="366BF2CE6FF622B1" modified="2013-10-16" version="1">MDKDFKTDLNNWALESIAFIALDTRLQCFDEKNKSPDADILINGMHKFFELSYELDKNLLYGDTFRHRNAAREKMKTQPPKAPTEMSILEKLIKIDRKTAVVMAMDMLFAGIDTTSTALTGILLCLAKNQDKQELLRQEVSQIKNNSLTANDMNNLPYLRACIKEGIRMYPVVSANARNISQDIVVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3P0</accession>
    <name>T1H3P0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02188734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3P0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010872-RA">
        <property type="protein sequence ID" value="MESCA010872-PA"/>
        <property type="gene ID" value="MESCA010872"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145021_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010872-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27190" checksum="A0443CE7BAC33A5C" modified="2013-10-16" version="1">MEKLPKADTINEASSEPVIADSVQPENVEEITEIAEEENASEEDMACKQSLEVVENSESLKENEELGIVPKEIENEENESNAVVNEIEQVTEGATPRRSSRRRSITSPLTTKTPRRKSIKITEVVEEVDEDTEKLPATDTIDQWNSEPTFEEIADEQRLDVTQNSKAVKEGMEHNTEKQPNDEQFEVVNDSVQDDDDAEIVKISEGVSAGVGIIEKSNIETIKESNEEQSNKDTQTVETNNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4Y4</accession>
    <name>T1H4Y4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02140896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4Y4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011356-RA">
        <property type="protein sequence ID" value="MESCA011356-PA"/>
        <property type="gene ID" value="MESCA011356"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1697542_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577364" evidence="1">
        <location>
            <begin position="28"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011356-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17859" checksum="3A95D5902090BB45" modified="2013-10-16" version="1">MIDMCFVLNVIKLITILYLLNCDLCHALSDEEITEITTEITVDSTTDIMEDFEEIKPPKISDSLKKILEKLNGTSDSGNNYEDIVFGNMESDEHYKNLYDYTMDENNICFLDNLKKNLLWWSHLNGSLITNGVILGQQFYYYTCKDNVSTLATLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H570</accession>
    <name>T1H570_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FMN hydroxy acid dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02193415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H570"/>
    <dbReference type="STRING" id="36166.T1H570"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011445-RA">
        <property type="protein sequence ID" value="MESCA011445-PA"/>
        <property type="gene ID" value="MESCA011445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631255_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="17251" checksum="D7CC5936FCC43E67" modified="2013-10-16" version="1">QWTFGPNIDVNDLKQFVQNTKLPVIVKGVLTGAGAVMAKTGGAKGIIVSNHGGRNLDSAPASIEALPEIVEAVGGSMTVMLDSGVRGGPEVFKALALGAKQVFVGSPEILLKSPPAMIKWEDFFSELLNGSNGIIARNFDNSTEETPPTRAIQRIKNSKTKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5C8</accession>
    <name>T1H5C8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5C8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011505-RA">
        <property type="protein sequence ID" value="MESCA011505-PA"/>
        <property type="gene ID" value="MESCA011505"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485915_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011505-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10046" checksum="EEF2E71E07917683" modified="2013-10-16" version="1">MPQHRRKIPFSNKKKKDQLLAKRQSRTFGKLLIHQRSKLSYLEQNKTRCCLLLNTSQCGLTPRSGNCILISSLILFDRSSACSKIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5M2</accession>
    <name>T1H5M2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5M2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012005-RA">
        <property type="protein sequence ID" value="MESCA012005-PA"/>
        <property type="gene ID" value="MESCA012005"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3020309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012005-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="56" mass="6607" checksum="5493D2E1E9A0F677" modified="2013-10-16" version="1">CMSKLLTLHQTVCLYLIRPDKQIHVFLSMVLLQLQTDNEFVRFPYSNPSGTYSPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Y4</accession>
    <name>T1H5Y4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. C/EBP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5Y4"/>
    <dbReference type="STRING" id="36166.T1H5Y4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012117-RA">
        <property type="protein sequence ID" value="MESCA012117-PA"/>
        <property type="gene ID" value="MESCA012117"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043327_3_0_1"/>
    <dbReference type="OMA" id="SPQMYDA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14693">
        <property type="entry name" value="bZIP_CEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334">
        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF69">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="210"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012117-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="287" mass="32912" checksum="C22A0EEB396797FD" modified="2013-10-16" version="1">NSANSILQKHIMQQYGHNELDELAAQEITLDLAHLIDDNFRESELGLFSDMVAATSPTTLSNTLQTNGVVAAAKVLQMQQQNTQQQWEYNLLAYTPQAVHSNATYTSNNSSDDNSSLASESSTIKEEPIDPQEYRRQFQEVTNNNFMSNNNVNGIYGLNAYGTNGCNNGFNGRTPHQNLPHLTGTHLAQLKHHRRYCPKSQAKQKSLDKTDEYRRRRERNNIAVRKSREKAKVRSREVEEKVKTLIKEKEQLLRRLEEMNNEMQVHQQIYLQLMNHANPEITRICGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H632</accession>
    <name>T1H632_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H632"/>
    <dbReference type="STRING" id="36166.T1H632"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012165-RA">
        <property type="protein sequence ID" value="MESCA012165-PA"/>
        <property type="gene ID" value="MESCA012165"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095001_1_0_1"/>
    <dbReference type="OMA" id="KDQHGYI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06526">
        <property type="entry name" value="metazoan_ACD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055269">
        <property type="entry name" value="Alpha-crystallin/HSP_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF34">
        <property type="entry name" value="PROTEIN LETHAL(2)ESSENTIAL FOR LIFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036514">
        <property type="entry name" value="Sm_HSP_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="74"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR036514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012165-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20882" checksum="761766ABE085B272" modified="2013-10-16" version="1">MSHVPLMYRDWWDEFDLPIRTSRLLDQHFGTGLKRDDLFSSAWTSSPSVLRHGYIRPWKRCNLQKQDSGSTLNVDSEKFEVILDVQQFLPEEINVKIVDKFVVVEGKHDEKQDEHGYFLDNSQDGTIYQAPTQTMLHLHSHLMVCLSAPMKAIPPPNVNRVIPITQTGPSQKDDHEKKAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6M8</accession>
    <name>T1H6M8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6M8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012361-RA">
        <property type="protein sequence ID" value="MESCA012361-PA"/>
        <property type="gene ID" value="MESCA012361"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830202_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012361-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15408" checksum="9D61BDF8FDE4BC6C" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVAERCKVLGITALHIKLRATGGNKTKTPGPGAQSALRALARSSMKIGRIEDCTPIPSDSTRRKGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Q9</accession>
    <name>T1H6Q9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diacylglycerol kinase accessory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6Q9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012392-RA">
        <property type="protein sequence ID" value="MESCA012392-PA"/>
        <property type="gene ID" value="MESCA012392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393321_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF54">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Diacylglycerol kinase accessory" evidence="6">
        <location>
            <begin position="14"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="21860" checksum="8E55021FD0B47D70" modified="2013-10-16" version="1">GGATQSEDNSQIFVMNNYFGIGVDADLCLDFHNAREENPNKFNSRLHNKSVYLRMGLKKMSRKSVDLSKELRLEVDGKVIELPTVEGIIILNILSWGSGANPWGPEKDDVFSKPNHYDGMLEIVGVTGVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXESEENNLDNNTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H759</accession>
    <name>T1H759_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H759"/>
    <dbReference type="STRING" id="36166.T1H759"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012541-RA">
        <property type="protein sequence ID" value="MESCA012541-PA"/>
        <property type="gene ID" value="MESCA012541"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_6_0_1"/>
    <dbReference type="OMA" id="WIARLIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012541-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18653" checksum="F50AAC339CCB6E13" modified="2013-10-16" version="1">VSTQGKMKLKFWHDSIDKCYSSEQSYVEDNPVLTELKNLQKALKRLVTSRDRPKNLGFLTTKQMEDYSEQTVSSLYYLLLEVWGVKDLNVDHAISHLGKAQGLTNLLRAIPYRGRNEALNIPQEVLMKHGVSQERVIRDKAGDKGVEECIFEIASIAHQHLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7C4</accession>
    <name>T1H7C4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">P53 and DNA damage-regulated protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7C4"/>
    <dbReference type="STRING" id="36166.T1H7C4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012662-RA">
        <property type="protein sequence ID" value="MESCA012662-PA"/>
        <property type="gene ID" value="MESCA012662"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2596905_0_0_1"/>
    <dbReference type="OMA" id="HRDLEHK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22860">
        <property type="entry name" value="PDRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030482">
        <property type="entry name" value="PDRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162:SF0">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10639" checksum="121BA4CF59F03911" modified="2013-10-16" version="1">AIREIEKNNEKKVWTTIGSLLVKLPREKSLELLRKDQIQIDTEINKLRSDQKVLVNKHRDLEHKTAYPGTHLKAMSHDEMSALKRNLPLGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA06</accession>
    <name>T1GA06_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA06"/>
    <dbReference type="STRING" id="36166.T1GA06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000049-RA">
        <property type="protein sequence ID" value="MESCA000049-PA"/>
        <property type="gene ID" value="MESCA000049"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_557344_0_0_1"/>
    <dbReference type="OMA" id="RKHENDI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="242"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000049-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="490" mass="55828" checksum="4320AD0E7D211C7A" modified="2013-10-16" version="1">MVARKLLSWGGGCEIDPKNKYSKLQIAIIKSTTSMDYDAIKYFNDLIEEYPDYVPARKGAAEAHFNLYAYNLTLNCLVEQKIIFKMQLNIWKKKVSAIWLWRLTGYIFYSAAKLPESLAFLKVSGSLVSRETVDTIVTVERKDLFDFAAKFLTFALKLSPNDNNLWYDISLCNYYTGVIFPEKSITKFDMALKSCMKSLQIQPTMWKSWNLLGVLNSTEIIDNLPMAQHCFIKALNLERKSAMVWVNLGSLYLKLTEIKLANEAFLRAQHAEPDYLCAWMGQAMVGELIGVDELEVMDLFRHCTQLNFHIESALGYAHWVCSILANEAKVKKNIYKYAIETMNAKALEKCVDTKERDKIRSNIGNILLKMDKPLEASEYFNSISEATFKSTIGLALAYYRANQHQESYSVYSSALEWLATSEEEKSLILIAIISLKNCPIQAFYSTCALGILHGDIQLSQTILGELLKIKDSEETCHHIAFLTSQVYKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAX6</accession>
    <name>T1GAX6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000392-RA">
        <property type="protein sequence ID" value="MESCA000392-PA"/>
        <property type="gene ID" value="MESCA000392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534296_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="19563" checksum="E15A91410160698C" modified="2013-10-16" version="1">MLNGGSSEVPKNFNSSSLKSPNVNWLSFAVSHRFGSSLIIITAGRFEFLLPIDVFGQSFLDFLEVEENKVFVDFFFTSMAHDVETFGCSLIMVVVLESPLFEIYEQISSSMLSTSISITVSAIEFTNDDEEVNSPIDPVCHLIFFGALTLPWNIMSNNDDWETAGFLRLGIFMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBV3</accession>
    <name>T1GBV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CTNNB1 binding N-teminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBV3"/>
    <dbReference type="STRING" id="36166.T1GBV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000742-RA">
        <property type="protein sequence ID" value="MESCA000742-PA"/>
        <property type="gene ID" value="MESCA000742"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870279_0_0_1"/>
    <dbReference type="OMA" id="RANIYED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000011">
        <property type="entry name" value="Pangolin, isoform Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF38">
        <property type="entry name" value="PROTEIN PANGOLIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTNNB1 binding N-teminal" evidence="8">
        <location>
            <begin position="16"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000742-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="14978" checksum="916E88883274A12D" modified="2013-10-16" version="1">MLEMPHNSHSSHTNASPGDDLGSTDEVKVFKDEGDRDDDKNSSENLFEEKSSLIDLTESEEKTGKITTRSEHNPVFGKIEPHTTGFNMGYFYSYTNGGAVANKMGLSPFFCHNGEALTTPPPAHCGIPPYQLDAKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCQ1</accession>
    <name>T1GCQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GCQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001071-RA">
        <property type="protein sequence ID" value="MESCA001071-PA"/>
        <property type="gene ID" value="MESCA001071"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1236299_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36695">
        <property type="entry name" value="AGAP008648-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36695:SF12">
        <property type="entry name" value="AGAP008648-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="1">
        <location>
            <begin position="70"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001071-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="25930" checksum="0BD838E29075F945" modified="2013-10-16" version="1">MRSFEKTSLLVLVICTLYGELVLGFNRNLPYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCKEYQIQGQYIYRDSFAISELKNNKPFDNEIFRLKLYVLTRNDAHIMITNEPGAQNGDAVYEMVLGGWSNTKSAIRDQKQRDNSVEKEGEVVSPLYPTEIVVVLRDDGELSVILPDFERPYMVYKDKSPRFWTYISFASWDDQPARWFYDCPLENDGPSEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCU3</accession>
    <name>T1GCU3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02035881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCU3"/>
    <dbReference type="STRING" id="36166.T1GCU3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001116-RA">
        <property type="protein sequence ID" value="MESCA001116-PA"/>
        <property type="gene ID" value="MESCA001116"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012949_1_3_1"/>
    <dbReference type="OMA" id="THTTIEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF27">
        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001116-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="346" mass="39809" checksum="FB9CDFEDC74EF2A4" modified="2013-10-16" version="1">MAGNPAPYSITPHFFSSFTDNMNIIERAMNTGLQVFSNMMHRIVVRLVYNKIQKHMGSNAIHPYELSKNVSFILQNGHATVSYPRALNPNVAEVACIHCKEAGPLPRDLESFINSSENGFIYVSMGSSVKASNMPERLRKMLVKTFARLPYNVLWKWEDSQMNHDIPPNVMLSRWLPQQDILGHKKLRAFITHGGLLSMYETVFHGVPVVTMPVFCDHDVNSAKAEVDGYAIKLHLETLTTDQLYKAVLKVIHDPTYKAAARARQRLLRDQRSSPLETAVYWTEYVLRHNGAYHLQSTARNLSWMQYYLLDVALLYFIVSYSMFHLAKRILRHDVIELLHKKHKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD08</accession>
    <name>T1GD08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD08"/>
    <dbReference type="STRING" id="36166.T1GD08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001186-RA">
        <property type="protein sequence ID" value="MESCA001186-PA"/>
        <property type="gene ID" value="MESCA001186"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="OMA" id="KEHFSTQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000042">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001186-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="51570" checksum="F6734787D9EC4245" modified="2013-10-16" version="1">MNYILRDFYEKFKNAGFPFSGFYLMSKPVAVITDLELVKNIMVKDFNNFNDRYMFFNEKQDPLTAHLFNLDGEKWKPLRKKMTPTFTSGKMKFMFPTIQGVSKELESAFEKSLNENSSVEIVDLLARFTTDVIGTCAFGVECNSLRDPNAEFRNYGRIIFTKGRHTFPPMRMFIMTFPRLARKLGIRVIPDDTHNFFMNVVKETVEYREKNNIQRNDFMNILMEMRKENESGMTIEEIASQTFLFFIAGFDTSSTTMSFALFELAHNQEIQEKLRAEILETLENDGGELQYESTNNMPYLDQVICETLRKYSVLSALTRMTQEDYKVPNTKYVIEKGVMVMIPIDAIHYDEDIYPEPNKFDPDRFTEENMKTRHPMTWLPFGDGPRNCIGLRFGKMQAKIGLISLLSKFKFSVCPETENPVTLNPRTFILSPLEGINLKVERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD78</accession>
    <name>T1GD78_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02110627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110632">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD78"/>
    <dbReference type="STRING" id="36166.T1GD78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001259-RA">
        <property type="protein sequence ID" value="MESCA001259-PA"/>
        <property type="gene ID" value="MESCA001259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109192_1_1_1"/>
    <dbReference type="OMA" id="EASIMMK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00916">
        <property type="entry name" value="Npc2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.770:FF:000001">
        <property type="entry name" value="NPC intracellular cholesterol transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033916">
        <property type="entry name" value="ML_Npc2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF68">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576831" description="MD-2-related lipid-recognition domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="7">
        <location>
            <begin position="22"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17319" checksum="6AA1E1947DB4234E" modified="2013-10-16" version="1">MNALLRIVFIVCLVTLGQATEVKQCPKSKTKRLSSDDVTLTSCTKGKCILKRNTDVDISMKFTPDRDFKDLNSDITGIILDVPLPFPGYYGSSACPFIHDAEGKEKVGCPLKAGETYTYKNSFKILPVYPTVNLVVHWGLGDKNGDAVCFEIPARIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDF2</accession>
    <name>T1GDF2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200237">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDF2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001338-RA">
        <property type="protein sequence ID" value="MESCA001338-PA"/>
        <property type="gene ID" value="MESCA001338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2475309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10229" checksum="4BEBD7EE33DCD9B5" modified="2013-10-16" version="1">MTILKFIRPIGIGIFDGLSVRSKRFEGACNMKDKKRSNRRSSKAFKERKDGNTIQLNRLKRSTAPLYQRSRDVILIRGIWRTMIMLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDL8</accession>
    <name>T1GDL8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDL8"/>
    <dbReference type="STRING" id="36166.T1GDL8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001407-RA">
        <property type="protein sequence ID" value="MESCA001407-PA"/>
        <property type="gene ID" value="MESCA001407"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944254_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="2">
        <location>
            <begin position="8"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7335" checksum="F4B40399B0E429E6" modified="2013-10-16" version="1">MFACMAAKTREDWKYVAMVIDRLQLYIFFLVTTLGTIGILMDAPHIFEYVDQDRIIEIYRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDP5</accession>
    <name>T1GDP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDP5"/>
    <dbReference type="STRING" id="36166.T1GDP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001436-RA">
        <property type="protein sequence ID" value="MESCA001436-PA"/>
        <property type="gene ID" value="MESCA001436"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091106_0_0_1"/>
    <dbReference type="OMA" id="NQDQPPH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000173">
        <property type="entry name" value="whirlin isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF29">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="33"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15037" checksum="D3877A2EF3E3C525" modified="2013-10-16" version="1">MQRLSNDQNGGPTTDQPIEHNPNVVPDHRGNLHVTIKKTKQILGIAIEGGANTKHPLPRIINIHEHGAAFDAGGLEVGQLILEVDGHKVEGLQHQEVARLIAERFADRNKADITFLVVEAKKSNLEPKNKALIFLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEJ0</accession>
    <name>T1GEJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEJ0"/>
    <dbReference type="STRING" id="36166.T1GEJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001754-RA">
        <property type="protein sequence ID" value="MESCA001754-PA"/>
        <property type="gene ID" value="MESCA001754"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004280_0_0_1"/>
    <dbReference type="OMA" id="WSCRCKF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010556">
        <property type="term" value="P:regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000019">
        <property type="entry name" value="18w protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000448">
        <property type="entry name" value="Toll-like receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000355">
        <property type="entry name" value="Toll-like receptor Tollo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF168">
        <property type="entry name" value="GH22922P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577278" description="TIR domain-containing protein" evidence="18">
        <location>
            <begin position="25"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="814"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="19">
        <location>
            <begin position="863"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1018"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1061"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1108"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1117"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1136"/>
            <end position="1170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001754-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1179" mass="134476" checksum="07981763B79B847E" modified="2013-10-16" version="1">MDYKFVVALIAILSLFGCPFTSLATAANQCSLEFNGTTSVTCNIRTIDKSGLDLLAADGTSRLDIKCNDVFLFESQLPQNVFARLQTLEELSIESCKLLQLPNNAFEGLSATKVLKVRTKNSEWGLGKSLELFPDSLIGLKQLTALHLKNLGFTNMVCNGGSELQVLDLSYNELKTIPENWGVSRFRRLQHLNLQNNNITELHAESFAGLSSLRVINVSNNHLETLPIGLFAGSKELREIHLQHNELYELPKGLFHRLEQLLVLDLSSNQLTSHHVDNTTFAGLIRLIVLNLANNALTRIDYRTFKELYFLQILNLRNNSIGHIEDNAFLPLYNLHTLNLAENRLHTLDDKLFNGLYVLSKLTLNNNLISVIEGSVFKNCSDLKELDLSSNQLTDVPEAIQDLAMLRTLDLGENQISTFTNGSFRNLHQLTGLRLIDNRIGNITVGMFWDLPRLSVLNLAKNRIQSIERGSFDRMWKSKQFDWIEISCQILMPNTFVHKGNLARVDLYANALNQLQLQLLRIAKIDEEQPQPEFYLGGNPFQCDCSMEWLQRINNMTTGHYPKVMDLQNIECMMPHARGSPQRPLTSLKSKDFLCRYEAHCFALCHCCDFDACDCEMTCPSNCTCYHDQTWGTNVVDCGGYQTSNLPKRVPMDSTIVYLDGNMFPILDNHAFIGRKNLRELYVNNSGVTSIKPKTFSGLTSLAILHLEDNKLKVLRLDGNKLHSIPIWQMQLSQFKTNIKSLYLGGNEWSCRCKFLQELTTFVADNALIIQDSQDIYCMDAVTGKKELDFNATSACSDYYAGGSVLQNGIPKTYLPLLAVALIFIFLFVILIVIFVFREPLRIWLFAHYGVRVFGPRCEESEKLYDAVLLHSAKDTEFVMQCLITELEAGRPPLRICLQHRDLSHEATHFQVLEAARVSRRVIILLTRNFLQTEWSRCELRRAVHDALRGRPHKLVIIEEPDVCLEAEMDVELVPYLKTSGVNRIRRNDRHFWEKLRYALPVDFPYRGNNYTIDHMTHEPPPPAYCPDGEDPNYSSATTATPSPRPSRRAVELLQAQFPQMSHHPNPHYQQSGHVRPPSEHIYSSIDSDYSTLDNENVMMMPQQQAAVNSLHRSMHQHQQQPQYNPHHTWRPAPSQDMQLQKQQQQQHHQQQQQQTPQQTTPQEVVPGQQGPHVQAYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGA7</accession>
    <name>T1GGA7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SLC26A/SulP transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02115994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGA7"/>
    <dbReference type="STRING" id="36166.T1GGA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002421-RA">
        <property type="protein sequence ID" value="MESCA002421-PA"/>
        <property type="gene ID" value="MESCA002421"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_824619_0_0_1"/>
    <dbReference type="OMA" id="RSINCEH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="6">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002421-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="337" mass="37661" checksum="82F174A7DC671532" modified="2013-10-16" version="1">MDFVSIQVSSGFTSACALIVFTSQMKNLLGIKAEGPTFLKMWTSIFQEIHHINWNDCFMGVGCIVFLLTLRFIGTLRSNKILWIFGISRNALAVGICLYIGYWSKSSGKNLFTLSGYIPAGLPEIKLPDFSIENQSFIELIQEMSSGLIVIPLMALLETYSACKAFAEGQSIDVTQELITNGVSNILNSFFQGYRINGGLTRSAINKASGARTQMSNFYIGFVVVISLLYLTPYFAYIPKSCLAAVLISAVIFMVQYKVIKPLWRSKKLDLIPGFAALLGCLIFPLHIGVFIGIGVDFIYLFYRFARPSIKVQVLKVSYSLHFRKIKNLKFLVPNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS7</accession>
    <name>T1GGS7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002606-RA">
        <property type="protein sequence ID" value="MESCA002606-PA"/>
        <property type="gene ID" value="MESCA002606"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017851_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577374" evidence="1">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002606-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13607" checksum="C48BD669F041CCEC" modified="2013-10-16" version="1">MLGYRIVLLACFFMIIASSSARPGIIPDLYKGIFGKSDKDYDKGYEQALKDRAYYGQMNVHPNQPYYYPANQVPVYPNQPHYQTVPLVSVHPATGHTVGGTGAPILGIFKSSLAIIMFGSNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHW5</accession>
    <name>T1GHW5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHW5"/>
    <dbReference type="STRING" id="36166.T1GHW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003028-RA">
        <property type="protein sequence ID" value="MESCA003028-PA"/>
        <property type="gene ID" value="MESCA003028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_11_1"/>
    <dbReference type="OMA" id="HMGEYDP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005353">
        <property type="term" value="F:fructose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990539">
        <property type="term" value="P:fructose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001511">
        <property type="entry name" value="Solute carrier family 2, facilitated glucose transporter member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF128">
        <property type="entry name" value="GLUCOSE TRANSPORTER TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="145"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="67"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="536" mass="60082" checksum="E76F8709A6E84ED6" modified="2013-10-16" version="1">MTHHFIVHFLVFPAKSTFLSHFDISNTNLFTYTFSLQTQVSIMSMNLSNKLDVLQRGDKHLETTVALNDIRTQLQELTKSVESCQSEVSEVKRDMVHIKHELDTVQQVKEEIEELREYVDRLEEHSQRRKLRLLEQGLTFSLTYAIFAAVLGMLQFGYNTGVINAPEKNIENFMKDVYKDRYGEDVTEDFIQMLYSIAVSIFAIGGMIGGFSGGWMSNRFGRKGGLLVNNVLGITGGLLMGFTKVSHSYEILFLGRFIIGVNCGLNTSLVPMYISEIAPLNLRGGLGTVNQLAVTVGLLLSQVLGIEQILGTNEGWPILLGLAICPAILQIMLLPLCPESPRYLLITNNGKKKQGRVTLRRLRASRMIDEDIEEMHAEERAQQAESHISPMELICSPTLRSPLIIGIVMQLSQQFSGINAVFYYSTSLFISTGLSEDSAKFATIGIGAIMVVMTLVSIPLMDKTGRRTLHLYGLGGMFIFSIFITISFLIKASIPNVYVYDTIYRSVNPTNLGKFVSSQREVERILKNKSNPKLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHX7</accession>
    <name>T1GHX7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02141587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GHX7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003040-RA">
        <property type="protein sequence ID" value="MESCA003040-PA"/>
        <property type="gene ID" value="MESCA003040"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027074_15_2_1"/>
    <dbReference type="OMA" id="LMKSEHW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008579">
        <property type="term" value="F:JUN kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003040-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8860" checksum="D90A630A2EC973C3" modified="2013-10-16" version="1">MREVFEDTSEFIGLTLNTGGVVLVHCNAGVSRSASVIIAYLIMKKGMSFDEAFKLVKDKRPAIQPNSGFLEQLXEGSSLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHY4</accession>
    <name>T1GHY4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHY4"/>
    <dbReference type="STRING" id="36166.T1GHY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003049-RA">
        <property type="protein sequence ID" value="MESCA003049-PA"/>
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            <dbReference type="Pfam" id="PF23415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003055-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3637" mass="404491" checksum="1CE693B62C981BFB" modified="2013-10-16" version="1">MDIQKESEIVAEECADERNGVNGDSEVKEDAPSNSGLVGPPSPLTGCYLMIILAEPHSNHHKDILIQRLIKGFLSWDSSDCHVDLAHELNTIAKQNPETEEGRNGEKLIQYASENLVTEILIHPTFSTLIQCMRNMLSSFTRHKHIIHSGYTFSGNGSWILQRVLYAYPNTITINIHCSDSGLWNNLPEKSYTKICKIKINPVDVLDSGDERINNFIDFLSPIIVPSSINDLLETSDVVGNIRFSHPTLYVFPGGQGDAALFGINGFNMLIDGGFNRKACFWDFVRHLDRLDAVLLTRLNNSTINGISAVLERKQDIQVYPQIGHFFCNIPETANSYSPDGGKDKDQLIVDLMERGYSLVQNLKNLNLKPHQCYNSSEPINLYHKVGHGKLDMYVIFTCSQSSLQALNISKSKKTSKASIIEKSGKGLPEKDIIDTNKPKSNNEIVVSNIKDNISTDDNIETTKKEEPNKVKTKTQKMTIKNKAPKTAEKKIMKEAVASEKKVSPATTPKKIMKYKSIPRTNKIIGDTPPKSTKDANNLKVLESKQKSLKSTKKDITKTTSEKKETKSERKPISRRPRGLSPVKKAPCSPIKTTKSKSDSKRSYIEKDGATADSSGISTPSIDDTSKKIDDTSKEIIDEEKQRELDDLKEEQEVVREIEAVFNRSEREAKRKIIKEDSTTEVEEEEEYLIIEKEEIEQYTEDSINEQESSVTKEEEFQKHQRDSQESEKQRKASTIENLNKELQEIISTARDIIINENEEAEESSQIIEVDEGKIEKIDIVESKEAVQQESVPEDKFSVTVESGATTTAPTLPEDERLTMDEIKENVVEEKYINEETKENEKDTKNDICKVPTTLTKCTLPIKMSFESKTVLLKDMVKTPDEVADLPVHEEVDLESYEKDDKSKDQNAELVTSGNEQLSTKEATNLLDNEKDNVGSNVDEENIVKDSNREATEERIDELVEEQVDKPVKVISDEVETKDEKEEEKQEKKLDVTEMEPKEDAEEEQEKKFDVAEIELKDEDLAEADIPKQEELDASASNIKDTIVSSPSDKKIPILELKQEEYDLEETKDKGLLFRKLNSTVSLTHLILDTTASSPIDKALPEIELLEKDIAETKTRDVTVSSPIDEAMSNIQLFSTEKGKNEEETDQYIIHSIKDTVSSPVDLQEFDISQAVIESELRETHITSVCSPIDDAKDKVSMDILHDIAEDDEERKLSTPSIELNVQADELRPKSPREEEIAKIVADVAKVLKSDKDIHEIIPDFYEKEASRSTSPKRESIKSSLESIEIKKEDISEENERKLDTEKEKSVEEDNVMNKNIAKIVFSEESDAKDVESEKEKLTHKDSKDDITVICDKQLDVEPEAEIKEASKLSSPRDLPAVDDDPDKEISKASSPRESLIEETFVEEDQEKKVSKNSSPRGSLLGESEKSVELEIEKDLNKSSSPRSSLLDEVEKTLEKVESEMEVSKSTRPSIVKEISSSSLEDKKEVVEQLKDVSKESSRRGSLHEDSIKPVEEDGKETSKASTPRSEKSKIEPISPSSSILAESEKSVCEEPAKEKSKDSSPKEELIKKEEEHFKETSPKSEKSDEAAGKISKELSPKEEESLKDMSEVVSPKSEKSKSEEPAELITKDFIPKEEMTENEELKKESTRIRAQQVLCLEKRTEFKEQSYLRDPVQEIHCLENSRNLDSLLGETDKAQAAELSKGSSPRDSLLGEIEKSVEEKLEKDISKTSSPRDSLHCEIEKSVEFEAEKKSSIASSPGSSILGKESDKDSLKGDEKKSSKVPSTEGSLFGESEKSVKPEEDKDREFETDIDKKSTPRVSLVDEVEKSTDLKIEKETSGVSSSKDTVSAELEKIVDVQIDKEASIMSSPRDSLPGESEISEKAETDITKDSPIKDVEKSEASKEDQQQTKSSSPRQSLGGESEKSTSIKEISKASSPRESLGGESEKSANLDKEIITSESDKHKESKGSSPRESLHGESEKSFEIAIGKEICKESSPRDSLTGDMDKHSGINKELSIAEDTVELKVMSQSSSPRESLLGEPEIQKSVEVELFKEPSKDSSPRESLKEELDKSVAECEKEDSKSLSPIGSLHAESEKSIDAEKSKDASKSSSPKGSLLGDLEKNKETEPEKEVSQSSSPRGSLLAESEKSMDTDKAKDLSKSSSPKGSLLGDIGNIDDIEPEKEDSKRSSPRGSIVAESEKSVDDEKIKDVSKSSSPKGSLHGDVEYLAQLEPEKEDNKSASPRESLHAESEKSADNEKIKDTSKGSSPRGSLLGDLEKDEEQEPEKETNKSMDAGKSKDVSKSSSPRGSVLGDLEKDKETEPEKEVSKSSSPRGSLLAESEKSMDTDKAKDLSKSSSPKGSLLGDIGNVDDIEPEKEDSKRSSPRGSLVAESEKSMDDEKVKDDNSSSPKESLHGDVENLAQLEPDKEASKSSSPRGSLHAESEKSIDSGKIKDTIKGSSPGGSLLGDLEKDEETEKEQEDSKITTPRSSIFAESEKSMENEKVNDISKSSSPKGSLLGDVDNLTEMELEKESSKTSSPRGSLIAESEKSIDVSKAKESSKSSSPMGSLLGDLEKDEKTEPEKETNKTSSPRDSILTDSEKVKDANNFDGIELENEAGKSSSPIGSLLGESEKSIDVEKVKDGSKSSSRRESLLGDMENNFGTEPIKETSPSPVGSILAGSEHSTGSEKVKEASKSSSPRESLHDIQSEKLTSQESLTKETKVISLTGDLDSSKEFVEEAIAIEDSPKAETEACLKYSEGSSPRGSLSGLDEIEESKKRPSHESASLSQTDKKDSIDSIKKDSLPDDLDQHTLEEQISKDLKEDESVKSESTSEKVTVSDISILQAVDDKKEISLALKDNCLTSAYVESDIKKDTIKQEIKITDKAPDKSIPTEKVKEGIASDSETENLKERINIDKTQEKDNEDKMSTKDMPIGQEDRKKDSMSSSPSVSVKDYSKGDICTSHESLIEECIIDVQEDNDKANINQESSVKTSSLKGELSTSEVIHEQVIDKQNLAVDKSKSVTSPQEYLNEDEKAIEDQSDKVNSDVLKESKECDKSSNEINIQISSIKSSKDSSPSVSEKDQIKDVRSSHESIAIQNVSPSEKNEINASNVSLSESKTVQIESKEESVTIPEKKDVEMKKEDIQLEPDNSKLKEFDVLKVSDKDCSISIEKHSQESLEIKLEGSSGDKQSDRVEEKESSTVSSPRKSEGDDLLELKSSQDDLVKDINIPKDTQNKELENVKDEDIENTKTDKSMSKEERSSEIKLSEVITTIKSVEIFKDSIADVKESRSMSVSTIIGKSSSEKEDETVHQTPPTAPVSPDKKIEESLHDDRDSKDLNEDEEEDDDCNLRLKYLKEDPMSTSVYGELADSLNIQGDSKEDSKELTDVYKFLDEAELNFKKALDEHVQLRGAEVMTTVTSKYEVTEFEPVKEIPGEKVVLDEAPKTSTKPDENDKKSKTEKDDDNDDKEVKEKWGKPSSLPSPAPEEFRTTPKKEKRAVLLKTKINNEKNLKKMSDISNKEKGGTPIYVDLTYVPHQGNGYYSHLEFFKNIRARYYYFRALTPIGRYSKLCLTPNSYGKIKVTIIPTYDTDTLGYWVSQNEELLAQNKIDLSPSASRCTINLQDHETSCSAYRLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI28</accession>
    <name>T1GI28_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003095-RA">
        <property type="protein sequence ID" value="MESCA003095-PA"/>
        <property type="gene ID" value="MESCA003095"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963449_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003095-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6779" checksum="57EE1A352AEF00A8" modified="2013-10-16" version="1">MVNEVTEDPYTSRYQITSRHSQQDDCLNHENAGIDRKNMITNKSSEILGYADHINIVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI92</accession>
    <name>T1GI92_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RDD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI92"/>
    <dbReference type="STRING" id="36166.T1GI92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003161-RA">
        <property type="protein sequence ID" value="MESCA003161-PA"/>
        <property type="gene ID" value="MESCA003161"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053631_0_0_1"/>
    <dbReference type="OMA" id="FPICVVM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039871">
        <property type="entry name" value="FAM8A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659">
        <property type="entry name" value="AUTOSOMAL HIGHLY CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659:SF5">
        <property type="entry name" value="PROTEIN FAM8A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="106"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003161-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="306" mass="35004" checksum="5C846851AB22F225" modified="2013-10-16" version="1">MEEKKLPEKSEKECYFEQLQKWVDMANLSQKAATNFPYFLLSNYPQLFQPSSPTSARFPIPNVQNGAAFRGRVPGNNENRMDDIFNNQARMESIIARNGGYEFVIAPFWKRVLAEVIDVLILMFLKLMVTFTIIDLFDLDLSIDFINLDSLKKTFEDDYSEFLSFSSDLLIFEVITKIAVCFYETLWTMYGGNNTIGGATPGKIIMGLRIVHVDAVVILPPQPQPQIYNLNNNGRQPIRALLYPAQNPGIRRAFIRALAKNFVITLMFPMCFFVLLFKNNRTAYDVLSKTVVVEENHMPVLRRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIR1</accession>
    <name>T1GIR1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003346-RA">
        <property type="protein sequence ID" value="MESCA003346-PA"/>
        <property type="gene ID" value="MESCA003346"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429570_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003346-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10494" checksum="BA7A917299E4B29F" modified="2013-10-16" version="1">MKALNDRLRNSVDNSRSVPRSFPRQNIPGAHSSHHSHHHHQHQHSKQQQDNAQPTHMMFTKDSVFIPPAIPLPYEPLNQTQLLQTQLILTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ94</accession>
    <name>T1GJ94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ94"/>
    <dbReference type="STRING" id="36166.T1GJ94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003538-RA">
        <property type="protein sequence ID" value="MESCA003538-PA"/>
        <property type="gene ID" value="MESCA003538"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_995957_0_0_1"/>
    <dbReference type="OMA" id="AMECIQD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000003">
        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="108"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003538-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="280" mass="31891" checksum="050C55BFF21715D7" modified="2013-10-16" version="1">MDKEPKPRIEPVVEPVNGIVQPPVIPPPERPGRVTNQLQFLSKTVMKFVWKHQFAWPFQQPDTPSFSSGPYSHSLVDGGTPNQLKSKEKLSEPLKYCNEILKELFSKKHSSVAWPFYKPVDAEMLGLHDYHDIIKKPMDLGTVKRKMDSREYKNAAEFAADVRLIFTNCYKYNPPDHDVVAMGHKLQDVFESRMAKVPEEQINNAFDKHHSAGSSDDDDSDTEEESHSDTERNAKLKLLEMKLIELQNEMSKLSEEAKNKKKAKKKNKKNSNISSAGLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ97</accession>
    <name>T1GJ97_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ97"/>
    <dbReference type="STRING" id="36166.T1GJ97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003541-RA">
        <property type="protein sequence ID" value="MESCA003541-PA"/>
        <property type="gene ID" value="MESCA003541"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_630537_0_0_1"/>
    <dbReference type="OMA" id="KHGENWE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015395">
        <property type="entry name" value="C-myb_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09316">
        <property type="entry name" value="Cmyb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="6"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003541-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="435" mass="48820" checksum="E4795AE7EB054732" modified="2013-10-16" version="1">MAQPSEPKYKENSLDEEEDQMIFQLHQQYGNQWAKIARRLPGRTDNAIKNHWNSTMRRKFEYNDGQRQRIIPLGQQLAANTTTQDLPKKPVDLDTSRTNLKALIKNGGHTKTSALQASLKNRQSIIQNFSKSRMPNILKSSKKMNLQPGCQISGSPQYTPSYPEGSVVYSEEMILPDSPKATPIKPLPFSPSKFLNSPNVNFEDFLSSSTPVRKIGSKEIKKELDTSSLETPNKQLLGPRTPTPFKDALAEMGKKRRGARYVPPSPSSLVEDIAEMIADDSRVEKDSNSSNEQINDSMKSGTSQPQPKRARKSLAASWVNDVKQEPQSEKKAPYETETPSKFLNSVDSAIFSPPSALKETLADSDLLINYRKENIPNSSKKRAQLEQQMVVDTRWAKQACGKTQDQIFMTNEAHLFLERKSLAPRSLNFFAGSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJV0</accession>
    <name>T1GJV0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJV0"/>
    <dbReference type="STRING" id="36166.T1GJV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003754-RA">
        <property type="protein sequence ID" value="MESCA003754-PA"/>
        <property type="gene ID" value="MESCA003754"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097687_0_0_1"/>
    <dbReference type="OMA" id="QNTHSET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901096">
        <property type="term" value="P:regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019155">
        <property type="entry name" value="CLEC16A/TT9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF0">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09758">
        <property type="entry name" value="FPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPL" evidence="2">
        <location>
            <begin position="47"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003754-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="249" mass="29288" checksum="D7FB51DAB9C66037" modified="2013-10-16" version="1">MLRSRSFFGWNRPKNRLSLEHLKYLYSLLEKNTTVSETNRSLLVESLRSIAEILIWGDQHDSSVFDFFLEKNMLSYFLHIMRQKSGGSSFVCQQLLQTLNILFGTSEMRLLYCGSKVLVIGRDYLLSNNHVNSIIVHKFDFSDEDVMGYYILFLKTLSLKLNPHTIHFFYNEHTNDFPLYTEAIKFFNHPESMVRIAVRTISLNVYKVENISMLQFIRDKTAAPYFSNLVWFIGKHILELDNCVRNDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJY2</accession>
    <name>T1GJY2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003792-RA">
        <property type="protein sequence ID" value="MESCA003792-PA"/>
        <property type="gene ID" value="MESCA003792"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1135897_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588364" evidence="1">
        <location>
            <begin position="21"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003792-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="27469" checksum="17EBF27A74257C74" modified="2013-10-16" version="1">MLKLITALLILQSNGAFVNSTPILGSLLNTVGNLIGSIISLPILDNLFGNDGTDLKQQFQSMRPVCYDNIINADTSICVDRINFHYSRIQEYMNLLYKSFLRSPVPSSYSCQMLQLWNLLVNLMRFVVQMLLNGNNLPLKLFISVFFSDSIAGVGLDLMNFANNILGSLTRLPTICQLSRSDLEYQLRTLFENFVAFMGSYRVASITRFPREGAAVICGISNYIQHNAMLISDGNAYQFTKNCES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK03</accession>
    <name>T1GK03_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aurora kinase</fullName>
            <ecNumber evidence="14">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="14"/>
    <dbReference type="EMBL" id="CAQQ02019963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK03"/>
    <dbReference type="STRING" id="36166.T1GK03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003813-RA">
        <property type="protein sequence ID" value="MESCA003813-PA"/>
        <property type="gene ID" value="MESCA003813"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="OMA" id="YVDHWCL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032506">
        <property type="term" value="P:cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="18"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="147"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003813-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="285" mass="33325" checksum="ED52934D55707EE2" modified="2013-10-16" version="1">MMKHPAYGQVYQWSPEDFELGAPLGRGKFGRVYMARERMSGYMFAMKIMFKTELQRGGVQRQVLREIEIQSRLKHPHILRLLTWFHDHNKIYLALEIAAQGELYKHLRNSPGSRFPEPRAAKYTYQVADALNYCHVNNVIHRDLKPENILLTADDNLKLADFGWSAHTLSNKRRTMCGTLDYLPPEMVEGNSYDDSVDQWCLGILTYEFLVGSPPFESNDQEMTYEKIKKLDIRFPEYLSTGSKDLISRLLRKPSTARITLIQVMTHPWVRENMRKYEAMKAMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK97</accession>
    <name>T1GK97_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151592">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151593">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK97"/>
    <dbReference type="STRING" id="36166.T1GK97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003916-RA">
        <property type="protein sequence ID" value="MESCA003916-PA"/>
        <property type="gene ID" value="MESCA003916"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161242_0_0_1"/>
    <dbReference type="OMA" id="ADWNESI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003916-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12667" checksum="ACA2DDB6396915C8" modified="2013-10-16" version="1">MKCPGSDGILAEMLKSGGDIPAEHIHEIITDIWRTDILPADWNESIVFPIFKKGDSKECKNYWGISILNTAYKILSRTLCERLKPYITTINGPYQCDFMPGKEMTKFPQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL44</accession>
    <name>T1GL44_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LIMR family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL44"/>
    <dbReference type="STRING" id="36166.T1GL44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004233-RA">
        <property type="protein sequence ID" value="MESCA004233-PA"/>
        <property type="gene ID" value="MESCA004233"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029445_2_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008075">
        <property type="entry name" value="LIMR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12625">
        <property type="entry name" value="LIPOCALIN-1 INTERACTING MEMBRANE RECEPTOR LIMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12625:SF0">
        <property type="entry name" value="PROTEIN LILIPOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004233-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11358" checksum="BDB0232FD9A2C58B" modified="2013-10-16" version="1">MISLEKDKISIFLLLLMMLYTGSYILLARFRRRDREDLFSTDDDEILVYRISLWMCTFSLAVAIGAALLLPVSIASNEVLLLYPNSYYVKWLNSSLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL97</accession>
    <name>T1GL97_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major royal jelly protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160921">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GL97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004295-RA">
        <property type="protein sequence ID" value="MESCA004295-PA"/>
        <property type="gene ID" value="MESCA004295"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220901_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="MRJP/yellow-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009:SF7">
        <property type="entry name" value="GH10609P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PROTEIN YELLOW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004295-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="25167" checksum="273AD0860B08535F" modified="2013-10-16" version="1">MDGMFGMSLTPQSLNGERYLYFHALASEKENAVPLRAVNNATMWSGNPEAHPELFKNIGHRGVQCAAEAMDSRGNLYCCLMEPISIFAWNINTPYQKKFFKDIATNPEKLQFASGMKVIKNKQGQEELWVSTNRLQKIYAGTIDYNQVNFRLLAGNINELNGGGFGPTRPPFPVPTRRPGGYGXXXXXXXXXXXXXXXXXXXXXXGGGSGGVNQDLGNNLXGWKWRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL99</accession>
    <name>T1GL99_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004297-RA">
        <property type="protein sequence ID" value="MESCA004297-PA"/>
        <property type="gene ID" value="MESCA004297"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3244920_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="43" mass="4688" checksum="49FD88793B2DD422" modified="2013-10-16" version="1">MNDCLHCVPVAPPPSEECSYENLDTINSDTAIELLAPMPTRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLM9</accession>
    <name>T1GLM9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026965">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004441-RA">
        <property type="protein sequence ID" value="MESCA004441-PA"/>
        <property type="gene ID" value="MESCA004441"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087574_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004441-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13381" checksum="A04D8C217AABFC00" modified="2013-10-16" version="1">MVLKYTSIMKWGIASFGSIAQNKLANISFLFRIECLKPTNRIDILPSRSLAASYTRSYRLGETCFSNKSRFIHPLHMTYPSQSDPLSNVWKLKQLVQLIIVPDYPFTVDAYGTKDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLN2</accession>
    <name>T1GLN2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear receptor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLN2"/>
    <dbReference type="STRING" id="36166.T1GLN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004444-RA">
        <property type="protein sequence ID" value="MESCA004444-PA"/>
        <property type="gene ID" value="MESCA004444"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122099_0_0_1"/>
    <dbReference type="OMA" id="GCNEGDR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear receptor" evidence="11">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004444-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="14181" checksum="8E6530F1A9A910BC" modified="2013-10-16" version="1">MFLIKYFNEKGLYFLCLFFFFFSIFCYLFQKGDRLLDIPCKVCGDRSSGKHYGIYSCDGCSGFFKRSIHRNRIYTCKATGELKGRCPVDKTHRNQCRACRLSKCFQSAMNKDEYIMFLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLS6</accession>
    <name>T1GLS6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLS6"/>
    <dbReference type="STRING" id="36166.T1GLS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004489-RA">
        <property type="protein sequence ID" value="MESCA004489-PA"/>
        <property type="gene ID" value="MESCA004489"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067273_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588384" evidence="1">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004489-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13486" checksum="866619EC1C0653F3" modified="2013-10-16" version="1">MIQKSLITLLISILYFISVNEALQLGLSTKTPKVTKHKPKEQQSLTSLYRVNSTSGTTCILMQTDGLISIRYRNKLKEDAEADLYLPDSPEMSAECVESDSETFTMKFKGFILEILFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLT4</accession>
    <name>T1GLT4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED9</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02026472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLT4"/>
    <dbReference type="STRING" id="36166.T1GLT4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004498-RA">
        <property type="protein sequence ID" value="MESCA004498-PA"/>
        <property type="gene ID" value="MESCA004498"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1817785_0_0_1"/>
    <dbReference type="OMA" id="ESQDCNH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011425">
        <property type="entry name" value="Med9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039242">
        <property type="entry name" value="MED9_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20844:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20844">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07544">
        <property type="entry name" value="Med9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13747" checksum="A08E56B143579DD0" modified="2013-10-16" version="1">METQTQDIKPIINETQPSQIQENTLKIADLDIEILPTIYEIIRCVEKDPSDNRNVSQECSQKILELKRRFDTARSQIKQLSGIDFNKEEQLQKRELLRSQLKQKQELIAKYKNKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMQ1</accession>
    <name>T1GMQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02181180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02181181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02181182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMQ1"/>
    <dbReference type="STRING" id="36166.T1GMQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004829-RA">
        <property type="protein sequence ID" value="MESCA004829-PA"/>
        <property type="gene ID" value="MESCA004829"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010631_0_0_1"/>
    <dbReference type="OMA" id="SPRFCET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000149">
        <property type="entry name" value="Cyclic nucleotide-gated cation channel beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF1">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL SUBUNIT B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="522"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="586"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="714"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="122"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="310"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="849"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="607"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="856"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004829-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="919" mass="105578" checksum="46A0A023AE8129DF" modified="2013-10-16" version="1">MDKIERLRKPLNFENELLLKSSPNILENFPYEDFKEAIGRKMTSPPASRKEPMFSETIEKNMFMKNVINMKGYSGRYASPKEKEASVRRSLEEISKDIKEMEDFITVTETLIQKEKERDKELYARERKRRQEEKRRKSLQSPNKENKSPPHRRFSHGTTSPPTFKINSFKRNKNGSPKYHKSKLYFRNGKIGCVEIENIFGSNIQSTHALVRNILKYENEKPLVSPDSVRRALTETNLPGQVDLFEDIMEEKMMEEIGGDKIDDMDIASIPVEDPRMVFTHLQMSTVGMLMRSKEQVRHLVRRFTARTKKVKSRLELPPTPSSSASSPSPTPTGQKDDIKSQSPINKLVTRNYFQADTPKSKLLINLCGGGDDKTLDPQGKFYISWLCVVSLSFLYNAWVIPLRATFPFQTPENTNTWLTCDFIADTIYLLDLVFVKHRVMYLFEGFWVKDKNLTRKNYMRKLQFKLDILSLLPLDLLYFVLGTQAVYVRFPRLLKIQSFWEVFKLLDRVISSPHFVRIAKTLTYMVYMIHLTACSYYAYSDAQELGSNRWVFSGKGHPYVRCFAFATKTATSIGKNPKPEKEGEYVFMTAAWLMGVFVFALLIGQIRDIISTATRTKNEYRQLEDETLEYMRRLNLPGDIQRRVKMWFKFTWDQQRTLDESNILDSLPINLKTDIAISVHIQTLSKVQLFADCEEALLRDLVLKLRAVTFLPGDCQVQVMGGPNNDIVLATLYEGSVFGEISLLGINGADRRTADVRAKGFANLFVLSKADLNEVIVYYPNAQAILKKRARSLMRKNAAREREEARKSAMKADVVIGNPKTPETPPKLLRTVIQALPTESPAVMLITKGSRKRNKEKENRNNEVNVKVENEKRPKSPDLLSSIQDELKTNNKNINLTDSEKALILSSASQTSLDKVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMR4</accession>
    <name>T1GMR4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMR4"/>
    <dbReference type="STRING" id="36166.T1GMR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004843-RA">
        <property type="protein sequence ID" value="MESCA004843-PA"/>
        <property type="gene ID" value="MESCA004843"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_0_1"/>
    <dbReference type="OMA" id="NISKWAP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF280">
        <property type="entry name" value="FI21844P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="19"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004843-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="396" mass="44147" checksum="2A8EDD98D704144F" modified="2013-10-16" version="1">MTMKLSLNIHNFNAFRYIQCGLLFLGLFSALFDRGSTPIIVTALSKESPSEWKFQDRQYILSSFYWGYVVTQILGGYLANKYGMKFLFLISILGPSLLSLLMPTVITFGGWKAYAAVNMFKGLIQGLMYPAIYYNISKWAPKPERNIMSNIVETGFSWGQLLSPFISGKIAASNISWPGIFYLNATIGIPRRFQIRHGKGEKPHSSKSRKRSMEDPSDKPKPIPWTSILTSPPFLALILVTACQELGYTFINSEVPQFFNALFNLDIESNGLNTTLIQIPTLVMMHVYAIVGYILLKRRIVGLTVLRKSYNAIACIIPTAIYIALTQLDGSQQTLTMVLIAIIASTLPAQDIAYWINVIDLSPRFSAVLYGIINTTTSIMSMLSPLIIGSIVSEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNA6</accession>
    <name>T1GNA6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005050-RA">
        <property type="protein sequence ID" value="MESCA005050-PA"/>
        <property type="gene ID" value="MESCA005050"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898634_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588420" evidence="1">
        <location>
            <begin position="24"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005050-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15106" checksum="31F5146F9387B8AD" modified="2013-10-16" version="1">MNSLKYLMLVALSFAMIFRNAEADFFTKGTVRTVTYNENGDQVIHQREMTPEETSNLDRTLNQAKDQILGVGQEVKRALFENTQKVGDIRSLGDLKGQWHSAGQEMRRSFEAGAAKMKAAAADMRKKFETPFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNB5</accession>
    <name>T1GNB5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNAAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNB5"/>
    <dbReference type="STRING" id="36166.T1GNB5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005061-RA">
        <property type="protein sequence ID" value="MESCA005061-PA"/>
        <property type="gene ID" value="MESCA005061"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429568_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005061-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10502" checksum="F11ADA0F42EC8A99" modified="2013-10-16" version="1">MDCFKPLEQLKILRISGNKISSVENINFPKSLEELDLSFNKLENVANKGLESLKNLKYLNLTFNNITHIEAELLNNLAKINNLDIREIEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPE7</accession>
    <name>T1GPE7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02017798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005475-RA">
        <property type="protein sequence ID" value="MESCA005475-PA"/>
        <property type="gene ID" value="MESCA005475"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690659_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005475-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8369" checksum="6F9012F6530FC17C" modified="2013-10-16" version="1">MLTILRQNPANNNSASRRVLSINSADKRIDPGQMDRPSDKNVKKMLGLPTGKSSPRQEEMFSSRFDKNIVFMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPP3</accession>
    <name>T1GPP3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02061580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GPP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005579-RA">
        <property type="protein sequence ID" value="MESCA005579-PA"/>
        <property type="gene ID" value="MESCA005579"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471631_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46276">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46276:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="3">
        <location>
            <begin position="49"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005579-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9471" checksum="EB79E81796AA310D" modified="2013-10-16" version="1">MVKTQEKALEAMKDGVFDVLPEGSLDYLTSEDLRLLLNGVGDISVSTLIXFWTGSPALPASEEGFQPLPSVTIRPADDAHLPTANTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ24</accession>
    <name>T1GQ24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ24"/>
    <dbReference type="STRING" id="36166.T1GQ24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005722-RA">
        <property type="protein sequence ID" value="MESCA005722-PA"/>
        <property type="gene ID" value="MESCA005722"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034326_0_0_1"/>
    <dbReference type="OMA" id="RSSIVFH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005722-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="528" mass="60718" checksum="5DA8DC29B44A3DE2" modified="2013-10-16" version="1">MLYDENKNFCTISNFVAESDQTVHFVNNVNKHNVKYMKCYDSKARYIPYKLFENFPNLQSLDISNSYIEDISRNSFAAADNLLYLNMSLNRIKLLGPSAFSGAAKTLKIELAANEISEISANAFNGLEDMDKLVLSGNLIKKLDKVVFSENVNLNHIYLDHNLLEEIHPDLFNYCKHLRIVDLSNNRIKALKPNTFEGNFVLEGLFLASNLLESFNLTNLLVLNAVKNVRANNNEIEYLSIRNYENFETMSLANNRISDITNITKLNNLISLDLSSNKVGPTINLKTFYNLTNLRELRLRNISLPGIQFGMFSKQTKLTVFDISLNKLRDLNLDMLAPYMTTVKQFYIDGNSLTEIKGRTTFYHAFPYLYSIGLSKNKFNCTYLHHIITPPFLSPNVELHIEPIDSDIEKTHIRDITCEHNLEDDQDYDDVIGDTSKPKPQHHFSEFSENERNILLSKIHDQQEVLNSHLLWMKITLLSIVIAFMCFMAFKSTDYNRRLWRSVQHQNHVGDSSVVFRSTTTVNTTGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ42</accession>
    <name>T1GQ42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lon protease homolog</fullName>
            <ecNumber evidence="10">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02064828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ42"/>
    <dbReference type="STRING" id="36166.T1GQ42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005742-RA">
        <property type="protein sequence ID" value="MESCA005742-PA"/>
        <property type="gene ID" value="MESCA005742"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_1_1_1"/>
    <dbReference type="OMA" id="WLTNIPW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000043">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000002">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000004">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="15">
        <location>
            <begin position="90"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="14">
        <location>
            <begin position="736"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="196"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="216"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="500"/>
            <end position="507"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005742-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="849" mass="96955" checksum="AC6C743F544800B4" modified="2013-10-16" version="1">MSAVGNLSRLIRSRNLLLVASRQSSLRFTRIPQRIHQLTNKTSFNLCNQFSRNFCSDRGKDDEIPPEDLAPEIDNHLPATVAIPEVWPHLPMLATKRNPVFPRFMKILEVTNPMLIDLLRRKVKLNQPYVGVFMKKDNENEAEVVQKLDDVYGVGTFAQIQELQDLGDKLRLVVVAHRRIKITGQILEELVPEKKVDEPEVTDRRKRRKTRSAKPPRTEKVPAEETAKKTEETQEPSAEKPASDTPQPVLMVEVENLKHNDYKQTEEVKALTQEVIKTIRDIITMNPLYRESLQQMLHQNQRVVDNPVYLCDLGASLSAAEPEELQKIMEEMDIPKRLLLSLGLLKKELELSKLQQKIGKEVEEKVKQQHRKYILQEQLKVIKKELGIEKDDKDAIGEKYREKLKDKNVPQNVMTVIDEELNKLNFLESHSSEFNVTRNYLDWLTSLPWGVTSEENLSLDQATTILDEDHYGMEDIKKVSSNLLLQLKGSTQGKILCFHGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKLIQCLKKTKTENPLVLIDEVDKIGKGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVLDTIPEPLRDRMEMIEMSGYVAEEKVAIAKQYLIPHARKDCGVQEDQITLKDDSLEVLIKNYCRESGVRNLQKQIEKVVRKVAFKIVKKESENFEVTKDNLTTFVGKPVFSQERMYEQTPVGVVMGLAWTAMGGSSLYIETAERKQNQDSKEGTLNLTGHLGDVMKESAQIALTVARNYIKTLILTINSWRQATCIYTFLKVVLLKMVQVLVSQSLQLYYPWQQESRSNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ97</accession>
    <name>T1GQ97_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dystroglycan-type cadherin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ97"/>
    <dbReference type="STRING" id="36166.T1GQ97"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005801-RA">
        <property type="protein sequence ID" value="MESCA005801-PA"/>
        <property type="gene ID" value="MESCA005801"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049700_1_0_1"/>
    <dbReference type="OMA" id="NRQDFET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008908">
        <property type="entry name" value="Sarcoglycan_alpha/epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048347">
        <property type="entry name" value="Sarcoglycan_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048346">
        <property type="entry name" value="Sarcoglycan_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132">
        <property type="entry name" value="ALPHA-/EPSILON-SARCOGLYCAN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132:SF14">
        <property type="entry name" value="SARCOGLYCAN ALPHA, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05510">
        <property type="entry name" value="Sarcoglycan_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20989">
        <property type="entry name" value="Sarcoglycan_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577551" description="Dystroglycan-type cadherin-like domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Sarcoglycan alpha/epsilon N-terminal" evidence="4">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Sarcoglycan alpha/epsilon second" evidence="5">
        <location>
            <begin position="121"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005801-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="44056" checksum="32350501ACBB3D34" modified="2013-10-16" version="1">MFRFFLLVFIFLFQKSTSIEQGTVGEVFVYKITPELFVWNKISNNSKEIEFRFRPTLKDHPELPSWIGYIYSKQHKIGYLYGTPPEKLADKKISLDIVALNKKTYETKAVILDIFINSKLPAPNEVQLRIHNFNLIQLLEPAQITNLMNVFKLDLWTESYKVSSETWGRVPLTPNDKEGVIVHLSSSALFSGKLKDLQEEVRPLYKHSSCNYKRTSVQKIFENVGFKLDWCAFRIVGKDIAPEVLYHQQSSLLHQKASNNWIGLKKKDVFERNYIDEIAISLAVPGVLFTIGFVTLFSILCLKSEKLGSPYPQGTSTVQMVQCSENQTTTTLKSLNLKDSNALLDNLSYQSQSPNNSQSNSPGNTYLRPKPPAYGTLGRNSTSGGVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQJ7</accession>
    <name>T1GQJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQJ7"/>
    <dbReference type="STRING" id="36166.T1GQJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005906-RA">
        <property type="protein sequence ID" value="MESCA005906-PA"/>
        <property type="gene ID" value="MESCA005906"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_904829_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF46">
        <property type="entry name" value="EPS15 HOMOLOGY DOMAIN CONTAINING PROTEIN-BINDING PROTEIN 1, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005906-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34953" checksum="7F281D50D0A41B95" modified="2013-10-16" version="1">MMNPLDGHVVWPVPDNHSISVTLFKDPRTHEMEDKDWTDMTKLTPNDVVKNCKLAFDTAENLGIPRVIEPTDMSLLAVPDKLAVMTYLHQMRAHFTGKQLHIEQYGETCEESSYVIGDYKSDMDHQNILNLSHLKSTIQSHRNSIDDDKISPPIQSPNSIKDFKDMFSPTAKNIVHKAALSPAKEKITSPLNNNSLNKDKEQQQQTQSQSTLHNNNSNNKNLNNGSLMSRRELNDPFGSDEEEKVLEKKPVETSPPTSPLIDSSATQKILNRHKEMSEKAKAMREKLNICNGDNKSDNDNERQQKLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQV1</accession>
    <name>T1GQV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQV1"/>
    <dbReference type="STRING" id="36166.T1GQV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006016-RA">
        <property type="protein sequence ID" value="MESCA006016-PA"/>
        <property type="gene ID" value="MESCA006016"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055524_6_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF521">
        <property type="entry name" value="IP01817P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006016-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="297" mass="33156" checksum="795B49F1EAC89983" modified="2013-10-16" version="1">MVLINSTSRLTHRIPLGLPEISRKYFQLESLDKKSPWISTVILSIFTPFQKLIICAIKLDKIAYLSVIYQLSVPTHQQHSSYLECQKYKQFIVGAGLITIGSLFLSGASELPSDYDFAPAAVLPVLTIVLGSIIFVVSFFGCCGAIKENSCMLTTYTIFLIILCILQIAFSSYVWATRQNLYDEAEVYVQQGWDNRKDNQKVLDAIQILGRCCGKDNKSDYGLEPLPASCCGSANEQDKCPANQAYDTGCFSGFKDYFSAYTNWIIVYGYIIAAIELAGVILACCLKGTLNRHRRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR44</accession>
    <name>T1GR44_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR44"/>
    <dbReference type="STRING" id="36166.T1GR44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006116-RA">
        <property type="protein sequence ID" value="MESCA006116-PA"/>
        <property type="gene ID" value="MESCA006116"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294841_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006116-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11592" checksum="FC62CF4014322D9B" modified="2013-10-16" version="1">MYERKQGENFPRTFECGPGKSLNTILKQVNAKAHSSSFNVEAMMRISSRKQCLLISKEKRNCSNFDRAQVHCRISVTPERSPEVWGAKITKMFTKLLTLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRW2</accession>
    <name>T1GRW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRW2"/>
    <dbReference type="STRING" id="36166.T1GRW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006406-RA">
        <property type="protein sequence ID" value="MESCA006406-PA"/>
        <property type="gene ID" value="MESCA006406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_9_1"/>
    <dbReference type="OMA" id="IFAMSTR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="4">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14080" checksum="A7DFC7F29A5DCAA1" modified="2013-10-16" version="1">MRQQPSLVDIHVPDGHKFTICGDIHGQFFDLMNIFKLNGIPSPTNPYLFNGDFVDRGSFSVECIFTLFGFKLLYPDSFFLSRGNHESSNMNQMYGFTGEVTAKYTSTMADMFTQVFNWLPLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRY1</accession>
    <name>T1GRY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calmodulin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRY1"/>
    <dbReference type="STRING" id="36166.T1GRY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006428-RA">
        <property type="protein sequence ID" value="MESCA006428-PA"/>
        <property type="gene ID" value="MESCA006428"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_898007_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016286">
        <property type="term" value="F:small conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015449">
        <property type="entry name" value="K_chnl_Ca-activ_SK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10153">
        <property type="entry name" value="SMALL CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006428-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34293" checksum="CB2EDC2B6D4A4C1D" modified="2013-10-16" version="1">MDQRKLMDNANTITDMAKTQNTVYEIISDMSSRQDAIEERLTNLEDKMQTLQDNLELLPEIITKCLTAHQERIEQRRNFLHPDTAVNAIPQTPAPMYNPNQPIIFPHSRSVPTSNTASQAYHWPTSPILPPISSRTPHLVPETYTMSLPSGNYPAAPSTTGAAGLATSGAHYANASGNNPQTDEESASILSNDDTNTTTSPTEYYTATDSTANEFEYEFEFSSPSNSPKSSTSLERYIDVEPRVDTETDSVTVVVLNPTSSQTPPKHHHHKQLEGTVVLAIITRTTNSPENATQIKVHRPLQEGHLKNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSK8</accession>
    <name>T1GSK8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006668-RA">
        <property type="protein sequence ID" value="MESCA006668-PA"/>
        <property type="gene ID" value="MESCA006668"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707600_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006668-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8717" checksum="9F25E54FAA332796" modified="2013-10-16" version="1">MKGNVPLIIPWNCVKDSLKLRIVNQPRKWEKVWLRWLNLEEWVCLAVRRRQNKGAGVPKTLGIEINKESLKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSL1</accession>
    <name>T1GSL1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSL1"/>
    <dbReference type="STRING" id="36166.T1GSL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006671-RA">
        <property type="protein sequence ID" value="MESCA006671-PA"/>
        <property type="gene ID" value="MESCA006671"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_71_0_1"/>
    <dbReference type="OMA" id="QEESCHY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006671-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="322" mass="36500" checksum="F5F98A0176B2B5E1" modified="2013-10-16" version="1">FAAKIINTKKLTARDFQKLEREARICRKLQHPNIVRLHDSIQEESCHYLIFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHLNGVVHRDLKPENLLLASKAKGAPVKLADFGLAIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKAVDIWACGVILYILLVGYPPFWDEDQHRLYSQIKSGAYDYPSPEWDTVTPEAKNLINQMLTVNPYKRITAAEALKHPWICHRERVASVVHRQETVDCLKKFNARRKLKGAILTTMLATRNFSSKSVIAKNKDESAGSQLKESADSSNTTLEDDDVKSRRQEIIKLTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSS7</accession>
    <name>T1GSS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02085445">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA006738-RA">
        <property type="protein sequence ID" value="MESCA006738-PA"/>
        <property type="gene ID" value="MESCA006738"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2801346_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051367">
        <property type="entry name" value="mRNA_TranslReg/HistoneTransl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254:SF16">
        <property type="entry name" value="CBP80_20-DEPENDENT TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254">
        <property type="entry name" value="EIF4G DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006738-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7536" checksum="01DE884987BB4932" modified="2013-10-16" version="1">HGAPPSLVLLTLLSKCCEDCVKPPIRSLSEIECLFFVLTCIGRDLETQLPKQLEKLLGSVRDAFXIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTM4</accession>
    <name>T1GTM4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007062-RA">
        <property type="protein sequence ID" value="MESCA007062-PA"/>
        <property type="gene ID" value="MESCA007062"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1919454_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007062-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="15078" checksum="4CDF14601921002F" modified="2013-10-16" version="1">MVLQDTWTSVKAAREFCDKFQTLRGFRQEMLCRVVSSNDIDVVGGNQVLERETRSRGLMVNEDKTKCMLSTRNQSRHELLDPIFTIGVDANRCLFRLIRLLGSKQLSRQLILPVLLYGSESCNLKSADVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTW5</accession>
    <name>T1GTW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007160-RA">
        <property type="protein sequence ID" value="MESCA007160-PA"/>
        <property type="gene ID" value="MESCA007160"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3175939_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007160-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="47" mass="5270" checksum="21116A920C46AD2C" modified="2013-10-16" version="1">MPQVANVIPILMKQLAINARGNVSTHVMLLRVWNVSSLEFALVLIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU87</accession>
    <name>T1GU87_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hemolymph juvenile hormone-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02017101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU87"/>
    <dbReference type="STRING" id="36166.T1GU87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007296-RA">
        <property type="protein sequence ID" value="MESCA007296-PA"/>
        <property type="gene ID" value="MESCA007296"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069908_5_0_1"/>
    <dbReference type="OMA" id="FENSHAR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF39">
        <property type="entry name" value="CIRCADIAN CLOCK-CONTROLLED PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007296-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19630" checksum="81EDD150F9D72E05" modified="2013-10-16" version="1">MGRGREFKAIFSELSVRGGSNFIIDKLVANTSTISFDVYITIPKISFVGKYYLRIKILLLEIGGKGNMTGTFENSKAVVKMRGSRYMKDDVEYVKFHKMPMRISVDKMKLNLENLFNGDKVLGEVGNNIINDNQDLYLQEIIPGLEKGLSKKFLDIANQILETATYDEMFPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU88</accession>
    <name>T1GU88_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02017106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU88"/>
    <dbReference type="STRING" id="36166.T1GU88"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007297-RA">
        <property type="protein sequence ID" value="MESCA007297-PA"/>
        <property type="gene ID" value="MESCA007297"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="OMA" id="LHEVTIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22842" checksum="DB4DDDDE07F19916" modified="2013-10-16" version="1">MTRKLHEVTILKQVDPMTKTFKIIILGDTGVGKTALLNQYVKQTFIQDHKSTVGVDFFFKNIYLHWEKVHLQIWDTAGQERFNSLSSSYFRGAHCCVLVYDCSKKSSFDNLLFWRDKFLKSDTMTFVENFPFVALATNVTLKSTSAKMRFGVDTAFCNIAEIVKEYQEKWEEVDMESLKDFPDFEIQNNKKGFCFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUZ0</accession>
    <name>T1GUZ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CLIP1 zinc knuckle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUZ0"/>
    <dbReference type="STRING" id="36166.T1GUZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007563-RA">
        <property type="protein sequence ID" value="MESCA007563-PA"/>
        <property type="gene ID" value="MESCA007563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_285800_0_0_1"/>
    <dbReference type="OMA" id="EPHHSEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032108">
        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16641">
        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CLIP1 zinc knuckle" evidence="8">
        <location>
            <begin position="1018"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="domain" description="CLIP1 zinc knuckle" evidence="8">
        <location>
            <begin position="1061"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="583"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1033"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="8"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="305"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="621"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="734"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1083" mass="123597" checksum="3BA1F607E5AAC044" modified="2013-10-16" version="1">MKTLLSQNENFQKQILTKDEAIERLEEKVKSLDSEVLQAELAKKVEEIKELTKKLTEIEDYRTELAKLKAIIKENEAKISEEKTEQMSAQEKKELEIVEMKEEISQLSSELTSYQTKINSLEEDLSKTRQLKEEIEKSVEDLKTQNDLEKEKCTSLETKVANLENVLGKLSSDQENSGAECEKLNLEINSLRKEILEQTSEIQTKVAEIEQLKTELETSQTNSSSIKDSLEKIKEESTAQMKVLQDALEAKGKEFEVASKEIRMLKLDLEEGKSSYESLKNTLEGSSQETVKHYEAKLCELQSSKAVSEQEVQQMLEKLSKINEDFSKEKEERSEAVIQIEELQKSLKEKEDKLKLLSEKALTTSQSAEITDISKKLQASLEEVKNFSTESSELKKKLEDLNNQHKEKEESLSKKVLEFDELTRKFQALNTKCESLVEQNAAVQKDLESSRNSSSDSNAEISKLSADLTAKQNAYQELLDKTTAEQISLQTQIQETHQKNNSLLSEIADLKISHAQNLEKTVALNEKALKDLKDQFEEEKKHLHESLEKVESLKLSGDENVKTLETMVKALQSEVENYAKNLNEKEKEWTSKEESLMSDLKGKGDSESAFQNKVVSLSDEITSLKQKLAEKDEHLKTKEAELTKVSSEKHNLEKELNDEISKKSLESGELQASLSKLQLDLESKDKKISEFEEKLKSSEEELKKSGEKMVENESAALKKYDELENHMKMVEAANFELAAKVSQLNEDLQKAEAEKVDIESNLELFQLEADKANKELHEKQENLLKECETLKLSGAEGTKAAQDLIEKNSAVLKECEELKETISKKDSEISSLSSKVDSLSTEIDSLKKNSSEKIGETEKKLIEAQNEKESLSKIVSELKEVLGKKDNELKALNEDNDVMKQLMQNEEEIQGQLEKYQEMAQEHGHCANHIKELQKSVKNSEEIAKKFERELLETKLKLQSLEKLQLNNNEYSKTEGQSEDMQKKNDTLKAKVEALEQGPIDFTKKNLFEITKRKPAPRKFCDICDEFDLHETEDCPKQSSDSPVPFEQPGEKKERKIPEPRKYCESCEVFGHEVGECPDDEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUZ8</accession>
    <name>T1GUZ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUZ8"/>
    <dbReference type="STRING" id="36166.T1GUZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007571-RA">
        <property type="protein sequence ID" value="MESCA007571-PA"/>
        <property type="gene ID" value="MESCA007571"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124632_0_0_1"/>
    <dbReference type="OMA" id="HENVPLV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF352">
        <property type="entry name" value="BCDNA.GH04802-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="9">
        <location>
            <begin position="91"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007571-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="21741" checksum="2DC03EE2B0A2D35D" modified="2013-10-16" version="1">MIRFFLLEKYDYTGYHNSYQQHQQQQHVPQQKKQHLQQVMPRGVGVDLIEENYDIDEGILDEDDEDEECRTGTRTATKNHENVPLVIVFLILLSYISFGTVIFAFWENWSLIDGAYFCFVTLSTIGYGDFLPKQTLLQQNTPSFQLFACCAYLLLGLVLVAMSFSILENQLLWKCKRIAVRLKLAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVB1</accession>
    <name>T1GVB1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVB1"/>
    <dbReference type="STRING" id="36166.T1GVB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007702-RA">
        <property type="protein sequence ID" value="MESCA007702-PA"/>
        <property type="gene ID" value="MESCA007702"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519020_0_0_1"/>
    <dbReference type="OMA" id="GRSHPIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007702-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9695" checksum="9DA24363EBDD7DDC" modified="2013-10-16" version="1">MQNKNQIMADEKEIQEKTIAEDIVVTKYKSAGEIVNKTLKTVIGLCVVDASVRDICTKSDNLILEETGKVSFRFYIFFKSSFVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW53</accession>
    <name>T1GW53_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW53"/>
    <dbReference type="STRING" id="36166.T1GW53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008024-RA">
        <property type="protein sequence ID" value="MESCA008024-PA"/>
        <property type="gene ID" value="MESCA008024"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519026_0_0_1"/>
    <dbReference type="OMA" id="LEICKCE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF275">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008024-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9574" checksum="C304CE002D85620F" modified="2013-10-16" version="1">MLSDFPIRLEQNFITELPPKAFASFRRLKRIDLSNNNITRIAHDAFTGLKALSTLVLYGNKIKDLPSSVFKGLTSLQLLLLNANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW56</accession>
    <name>T1GW56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Ken and Barbie</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW56"/>
    <dbReference type="STRING" id="36166.T1GW56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008027-RA">
        <property type="protein sequence ID" value="MESCA008027-PA"/>
        <property type="gene ID" value="MESCA008027"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019233_0_0_1"/>
    <dbReference type="OMA" id="QQFVYQW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002034">
        <property type="entry name" value="transcription factor Ken"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002059">
        <property type="entry name" value="transcription factor Ken"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051497">
        <property type="entry name" value="Dev/Hematopoietic_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993">
        <property type="entry name" value="B-CELL LYMPHOMA/LEUKEMIA 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="13">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="136"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="391"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="492"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008027-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="623" mass="69022" checksum="75A9BB0484E57AEA" modified="2013-10-16" version="1">MTVFETVGHLRHLVARMLMLHYSKHGECILQEINAAFRGEHPTDLIIVCENKETLHAHKLVLAAASPLIRMCLEESPFNEPTTTVYFPDINAAYFRSLLDFLDLHPIERCCSITDLLSLLQIKSTDWRTVNSQTAALTTTSTSSANSHSRLKNRRQASSNFEDNNDNDNDSEHRSGEKNLEICSDDDMASKDGDNGEQEDMVNKKNRKNREGSSTPCFDMVIKREDQENTNSSNPENNQHHEDTENEDCGSSNNSEKGQKDDQSLENNDNDDNNDSERRSLGPVNLSIGSRNRNIDNKDDLQQQQQQIQHEAAMQFFDHFKTKRKALYFLPDSSAHHLLKPPPDQDILQNSPENYVVTPHRKRRPGFHNSPSTNQPFVPYHPSFLSEIRQKSPAAFSGSSSTDLFRQNGKSPTGEEGGGGNGTPGDGQSSGSAPPALPLPVPFPYHQWSQSTHSNLANLHNSPLSKLSPSAANSIEMQLAQLPNVRNAPSNGGSGGNGGGEGGSGSAGSGNPSAANAAVREYRCQYCGKQFGMSWNLKTHLRVHTGEKPFACRLCVAMFKQKAHLLKHLCSVHRNVISVNNGPNTSNRYSCCFCPMCFESSQELVRHLSGHHNNLLLTKNLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWE3</accession>
    <name>T1GWE3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02032327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008117-RA">
        <property type="protein sequence ID" value="MESCA008117-PA"/>
        <property type="gene ID" value="MESCA008117"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000526_2_4_1"/>
    <dbReference type="OMA" id="FERCTTR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="48"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008117-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="17237" checksum="2C1AA926AB02C234" modified="2013-10-16" version="1">MNSRPYILESDDPADDEGVTPNDILVSKNISVLNPGVFSETDSILKRQWRISQYLTELFWKKFIKIYIPNFINRKKWIQTCPNLKVGEVVVLNDGTSRGDWLIGLVTQVFPGKDGXXXIVRTADVKTQRGLYRRPTTKLCRTDIEAASLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWP0</accession>
    <name>T1GWP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWP0"/>
    <dbReference type="STRING" id="36166.T1GWP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008218-RA">
        <property type="protein sequence ID" value="MESCA008218-PA"/>
        <property type="gene ID" value="MESCA008218"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087356_5_0_1"/>
    <dbReference type="OMA" id="MSQIMMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF18">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="6">
        <location>
            <begin position="16"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11162" checksum="FA57E7E64A436761" modified="2013-10-16" version="1">MTKVEDLSNVEMSELDWIQLRKDMGPVGETFSDKFMRKVKENPVVPVGCLATTMALCYGLYNFRTGNRKMSQIMMRTRIAAQGFTVAALIGGVVMSFSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWR5</accession>
    <name>T1GWR5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWR5"/>
    <dbReference type="STRING" id="36166.T1GWR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008244-RA">
        <property type="protein sequence ID" value="MESCA008244-PA"/>
        <property type="gene ID" value="MESCA008244"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340234_0_0_1"/>
    <dbReference type="OMA" id="VMIENTC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008244-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="23892" checksum="BF19F400EEDE1659" modified="2013-10-16" version="1">ETNINCLGQLNALAELQGIGSLTIDSEGNCLTKSSSSTTWRLYAIYRLHHWGLKSINGSDVTEHEIELSHKEFMGLSDLVLWSLPEQMLQPLLQRLRLDETCSASKLSAKDWLMRAEQSLRIVVGKEALQWKRNPMDASRDRGKTHFSVMIENTCNSVEKLNKLNDLWPNMFFGLASFELHNTGIPDIVTGANVRYYIGHWQQVQNSLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX07</accession>
    <name>T1GX07_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GX07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008345-RA">
        <property type="protein sequence ID" value="MESCA008345-PA"/>
        <property type="gene ID" value="MESCA008345"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1251919_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008345-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25176" checksum="34838D8FBE08CEC9" modified="2013-10-16" version="1">MYLGLSPLDHSNSPLLCILLTEFVVGNDFEEYVMRPEAHFKVNNTPDSIKSALLITLIGPDAFSILRINLYVDYRLSDNNLRVALLKEKNLTFESACNLTLTWEVAQFENGNISSIILLSILTKFECTKCNRWYYIPDDCPAKKWICNFCKKQGHIASRCRNKSQETPTEHLSHIEELKKAVESIKNVRHCPLENPIQVEIEVEGKNLEMEVDSGACVSXN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX17</accession>
    <name>T1GX17_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008355-RA">
        <property type="protein sequence ID" value="MESCA008355-PA"/>
        <property type="gene ID" value="MESCA008355"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087576_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588595" evidence="2">
        <location>
            <begin position="17"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008355-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13163" checksum="0E035CEC0A0A538D" modified="2013-10-16" version="1">MEHGTSVIMLFLFSLARYPSEYTSIPVFQHADAITTVGRVFCTPGTLTASLCKIPAHLLAGFDDKNINYISPECAISDFFCFWFITLQIPAVCHRLFVLYISAMFNVLFGSVFYAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXK8</accession>
    <name>T1GXK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyisobutyrate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02136564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXK8"/>
    <dbReference type="STRING" id="36166.T1GXK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008561-RA">
        <property type="protein sequence ID" value="MESCA008561-PA"/>
        <property type="gene ID" value="MESCA008561"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_6_2_1"/>
    <dbReference type="OMA" id="RITHCGH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000006">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="8">
        <location>
            <begin position="3"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="9">
        <location>
            <begin position="58"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008561-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="17539" checksum="80D98886589A469D" modified="2013-10-16" version="1">MISEQGARFVDAPVSGGVPGAEQATLTFMVGGTHDEYTNVKNILECMGKRITHCGHYGMGQAAKLCNNMMLGISMCGVAETMNMAVRLGLDPQVFMEILNSSTGRCWASEINNPVPGLLPNAPAGRQYAGGFSTDLITKDLGLASGVATASNTPIPMGSLAHYLNIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXT7</accession>
    <name>T1GXT7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02132965">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXT7"/>
    <dbReference type="STRING" id="36166.T1GXT7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008642-RA">
        <property type="protein sequence ID" value="MESCA008642-PA"/>
        <property type="gene ID" value="MESCA008642"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1186186_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF73">
        <property type="entry name" value="KAZACHOC, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="37"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008642-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="234" mass="26082" checksum="71F7DE06F0A8F22C" modified="2013-10-16" version="1">MLFFIQYLFYFIADEALNDQSQNLSLEKSYSLESAAFTNFNTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFYIGTTLAAAMYIVGAVEIVLTYMAPWLSIFGDFTKDAEAMYNNFRVYGTGLLIVMGLIVFVGVKFVNKFATVALACVILSIIAIYVGIFDNYHGNDKLFPNISELVDPRIFVKDTAFIQIPHFDFYAVGILYNCTQDFYFPLNVFFAWDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXV1</accession>
    <name>T1GXV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116598">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116599">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXV1"/>
    <dbReference type="STRING" id="36166.T1GXV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008660-RA">
        <property type="protein sequence ID" value="MESCA008660-PA"/>
        <property type="gene ID" value="MESCA008660"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_864866_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008660-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="322" mass="36183" checksum="9241C1DA447A9BB9" modified="2013-10-16" version="1">RTGRSTTGTHRSTGVSATPTYLPNITEHVQATTPPELNVVQSPQYFPGGTKQPVQPIYGTRWSSQAPDGYLPQTGARWSQETGSDNEQIHAGYHHNHKPISPNPLPNPDLTDTSYLHQHQNKMNMPPSILENIQNVRNDNVSNGYDMERENLYGSMRRKDYQDNYGQYKTSQNGGDGRGGTSGGGGGSQVVNHFRTFHNESPYLMEKQRPVAYLGNKTDSPYMSKERITPLPDIYGSREPTQTHYSMKSPPLYNESMLKHQHQQSLDYHSDRMSEGSDKNGYHFPYTAEEDHISSNALNRNHNHNIVNDVNNPGAMTINSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY31</accession>
    <name>T1GY31_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY31"/>
    <dbReference type="STRING" id="36166.T1GY31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008751-RA">
        <property type="protein sequence ID" value="MESCA008751-PA"/>
        <property type="gene ID" value="MESCA008751"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943425_0_0_1"/>
    <dbReference type="OMA" id="ANKSCIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="29"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008751-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14263" checksum="E5A48E47986D9C7B" modified="2013-10-16" version="1">MIISNQQSCPSSTLMESTRTMLEGGGNGANSVEVSLGAWNLTEKNEKGRQTLVANKSCILPHEKYNSKTLLNDIALIHLPESIKFNDYIQPAKLPKFNSNGKYKNYVGEIATASGWGKTEAGRISNILLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY39</accession>
    <name>T1GY39_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008759-RA">
        <property type="protein sequence ID" value="MESCA008759-PA"/>
        <property type="gene ID" value="MESCA008759"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796858_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008759-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7988" checksum="525434C371A4E59E" modified="2013-10-16" version="1">MAPMMLSLNPPLLTPSVDDKLQTVTTEQSHMRMFKTWIYVKRLFIIQSKYSTALTVQKMEKWACRLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYK2</accession>
    <name>T1GYK2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02184141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184142">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYK2"/>
    <dbReference type="STRING" id="36166.T1GYK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008933-RA">
        <property type="protein sequence ID" value="MESCA008933-PA"/>
        <property type="gene ID" value="MESCA008933"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141232_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF376">
        <property type="entry name" value="CYTOCHROME P450 15A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008933-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18330" checksum="693A7A5DAE6AD61A" modified="2013-10-16" version="1">MVSPILVVAIVAILIFKGYKYMVSRPCGFPPGPPKIPLFGTYLFLMSLNYWFLHKAAITFSKWYKSKIIGFYVGTAATVVVHDDKVIKKVLNSQVFEGRPDMLVTRLREPNFEKNGIFFHDGEQLIETRRFILRNLRRLSPKKFICRLVYLCLGRQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ49</accession>
    <name>T1GZ49_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ49"/>
    <dbReference type="STRING" id="36166.T1GZ49"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009142-RA">
        <property type="protein sequence ID" value="MESCA009142-PA"/>
        <property type="gene ID" value="MESCA009142"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1152860_0_0_1"/>
    <dbReference type="OMA" id="VITHAGY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="50"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009142-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="241" mass="26009" checksum="7A7325B80AB75A45" modified="2013-10-16" version="1">MIRKYITESRSLVGAVLFYFSSVPTMKICLVLSLLLAVASALHFPLNGRIVGGEIATRGQFPYQAGLSTTKKGKSTCGGDSGGPLVATRFNYLVGVTSFGATSGVTKFVVWLGVNNVNTVSLQPGGLKFTITDQDNVITHAGYKALTLVNDIALIKLPRKIEFTEYIKKIDLPPRANKYPSYDNEPVVASGWGKPTDAATAITTVLQFVDLTVLDLKSCGRYYLPGLVKSSNICKNADYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ66</accession>
    <name>T1GZ66_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ66"/>
    <dbReference type="STRING" id="36166.T1GZ66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009163-RA">
        <property type="protein sequence ID" value="MESCA009163-PA"/>
        <property type="gene ID" value="MESCA009163"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064127_0_0_1"/>
    <dbReference type="OMA" id="TIHCIDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13568" checksum="E0B24B144963FB7D" modified="2013-10-16" version="1">MPQHNTGMGNACNLRLIQEKFESMTNETKALIKDNRFHGTTADIWSSRGRSFLGVTIHCIDSETLKRKSRALAVKKLSGRHTAYNIGIELQRIFYDFQLHKNKNVVTVTDNGSNFSRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H094</accession>
    <name>T1H094_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="2">1.3.1.89</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53364"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="3">
            <dbReference type="Rhea" id="RHEA:53366"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53360"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:53362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="4">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Dus family. Dus3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02127194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H094"/>
    <dbReference type="STRING" id="36166.T1H094"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009567-RA">
        <property type="protein sequence ID" value="MESCA009567-PA"/>
        <property type="gene ID" value="MESCA009567"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798302_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102265">
        <property type="term" value="F:tRNA-dihydrouridine47 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009567-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16848" checksum="EBDA3A05C75C31E1" modified="2013-10-16" version="1">MRTGVYADKSVAHELFPKVEQWGASAVTIHGRSREQRYTKNANWEYIEECASKVNRIPVIGNGDILSWEEYNEKKQIAPHVSSVMLGRGALIKPWIFKEIKEGKTWDPTSSERFEILQKFTNYGLEHWGSDTKGVECTRRFLLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0H1</accession>
    <name>T1H0H1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein asunder</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cell cycle regulator Mat89Bb</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Set apart in position or space protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Belongs to the multiprotein complex Integrator, at least composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7, IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13, IntS14 and IntS15. The core complex associates with protein phosphatase 2A subunits mts/PP2A and Pp2A-29B, to form the Integrator-PP2A (INTAC) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the Integrator subunit 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02383703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0H1"/>
    <dbReference type="STRING" id="36166.T1H0H1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009649-RA">
        <property type="protein sequence ID" value="MESCA009649-PA"/>
        <property type="gene ID" value="MESCA009649"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012654_1_0_1"/>
    <dbReference type="OMA" id="SPMHEKV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019355">
        <property type="entry name" value="Cell_cycle_regulator_Mat89Bb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12955:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12955">
        <property type="entry name" value="SARCOMA ANTIGEN NY-SAR-95-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10221">
        <property type="entry name" value="Mat89Bb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="540"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009649-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="579" mass="65246" checksum="930A08C00E89BA49" modified="2013-10-16" version="1">MLDTNHKTIFILDHTQYFSIPSENYIDLDCLKSKGTSSYAPTLSKSLWTCSVEGAIEYSRIVWDLFPKGKLIRFVVSDTTAHIINTWQTQNLHYILNAMSIVGVPQTHRHPGSDNSVIHGLKAAIESLFEPTEQQKELIKKGNTIQNKGRVVCLTSARDDGSMKSLEEIFLTVLMKQNEIVLKQQSELLPLDFCELVFINIYPRSMESFVSKKPATKVSPILSVEVHSCQAGDIPDKLTHLLLNHYDLASTTVTGIPMKEEQMPTPAQTMISHSVICASELLLPKSIKEGSDYETVTLKWCTPRGNGGELQPCLTMHRVTPVDVTSRPSSCLINFLLNGRSVLLEMPRQTGGKINSHLLSARGGEIFIHTLMVTRSCLEEHPAITEGEGGRVTDYRIQDFVQFAKNHKLFPLRQMNDHIRDENLLKARSKLFRHTRYFPLTTSSTIVYNLRNILDVFLQCVEKDEINDSQLMQCRQLIFGIMNSAVKQEQLPNLGIRGKSNRVRDDQQQRLLWMELELILQNSGKSPGHKALLKTVQECRSRTGNGESTIKTEEPNGRGALLRTSTDSPQSPPHSVEFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0H2</accession>
    <name>T1H0H2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0H2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009650-RA">
        <property type="protein sequence ID" value="MESCA009650-PA"/>
        <property type="gene ID" value="MESCA009650"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690653_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009650-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="9052" checksum="42E80D17D272B89A" modified="2013-10-16" version="1">MDTTSEGIRKELLSRYEPWDRIKSDWKETFDYRRNEIMTKKLVDIFRRWPKYCKEKGKEFVSGNGFCPLISREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H153</accession>
    <name>T1H153_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02387210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H153"/>
    <dbReference type="STRING" id="36166.T1H153"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009906-RA">
        <property type="protein sequence ID" value="MESCA009906-PA"/>
        <property type="gene ID" value="MESCA009906"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1050845_0_0_1"/>
    <dbReference type="OMA" id="CTFANCN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
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        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
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        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
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        <location>
            <begin position="157"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009906-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="265" mass="30536" checksum="C22AF01AA1F13E56" modified="2013-10-16" version="1">MDDEIKIKQEVIDSEPASNCETQFTIEEVGDEGCFQKCPEYPQSPLKHVGENLLQAGEHLIGKIQESSVKLTPSGKPKKEFVCMVPDCGKVYTKASHLRDHSRIHTGERPFLCSWSGCTKSFTRSDELQRHFRTHTGEKKYNCDKCDKKFSRSDHLKKHANSHIKFEKNSRNRGRTKSEKDDNSEVNTSVSRKRGRTKSKKDENPEVFTLVPAVQIKEEPFVEDEPIIEQQLTIKNEPVDHSDKEDSLIKEEIVIKEEPEFMINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1G9</accession>
    <name>T1H1G9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1G9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010030-RA">
        <property type="protein sequence ID" value="MESCA010030-PA"/>
        <property type="gene ID" value="MESCA010030"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1316741_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010030-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="24253" checksum="20C73289999A08F9" modified="2013-10-16" version="1">METSTSSNPALNDISEHEESHNDSVLDTNYKQSSNRRSSLGIFRKAEFEKSSRRRTSLANPFVLFGRKDSHKITSLYENAVKFEKSDGSLNTSQDDYDESEDHFHTSSPNNDKSKKNGRFVYFNDSNTSIVSNEPDNEEPDIDLASSKSYRRRSSVQIKAERKKADTQLNYSVNDISSIEADQINAYYQQRQKRHSWWNIFANEPRKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H241</accession>
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    <protein>
        <submittedName>
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        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176645">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H241"/>
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        <property type="gene ID" value="MESCA010273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375173_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10915" checksum="2EB27EB5D3F9EB96" modified="2013-10-16" version="1">MLVVLIWSVLRLPKIDEDVGRVNPGSTYTILNLFLLTIIIQNFSRIHLSVKLKITGKLHREIFPRNIFLGAIFKVCVFEASFKVMVLIFSEHICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2M1</accession>
    <name>T1H2M1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2M1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010471-RA">
        <property type="protein sequence ID" value="MESCA010471-PA"/>
        <property type="gene ID" value="MESCA010471"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402147_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010471-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10441" checksum="9AB083A2B7E2B796" modified="2013-10-16" version="1">MEVEIYRIRAFNICRFRVYPDHTMHPVNAFNEMQAHLGIVALHIARLSTSRQCMKAITQSITGGVSLRGTHKDILDILQVGQAFALDFRGGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H303</accession>
    <name>T1H303_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146796">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H303"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010612-RA">
        <property type="protein sequence ID" value="MESCA010612-PA"/>
        <property type="gene ID" value="MESCA010612"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1273522_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577334" evidence="3">
        <location>
            <begin position="23"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010612-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24035" checksum="0EE05D4523EC9082" modified="2013-10-16" version="1">MNFLMLHEFDVMLTMVMTSSSATPINISVVDDAETDKIADKIADAEEVLKSLAEESNTSKNSSMIIPFNEIIRNMYNSPEDSISIENSASSPNSVIMETIDVSDDMNTLLNSTKETIPNYTNLDTQPTKSETFTMDASFLYEDVQSQCIHRPCQITPLLPHKAGPGRKKKTNGNIQQHPMSPSISVVSSSSPAQTVQLKEIQEIFSNTQIPIPQFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3D9</accession>
    <name>T1H3D9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ras-related protein Rab-21</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H3D9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010764-RA">
        <property type="protein sequence ID" value="MESCA010764-PA"/>
        <property type="gene ID" value="MESCA010764"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="OMA" id="YCKDQFD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04123">
        <property type="entry name" value="Rab21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000550">
        <property type="entry name" value="ras-related protein Rab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041833">
        <property type="entry name" value="Rab21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="200"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010764-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="237" mass="26542" checksum="CA15BB7C00D8D507" modified="2013-10-16" version="1">MAATAPSKDYNFKVVLLGEGCVGKTSIILRYVEDKFNSKHITTLQASFLTKKINFDGKRVLLNVWDTAGQERFHALGPIYYRSSNGAVLVYDITDQDSFEKVKNWVKELKKMLGNEVVLVIVGNKTDLEKDRTVDSQEAEDYATSVGAKYFETSAKMNEGIEXXXRKLKTMQQVSALTKMNEGIEDVFLDLSAAMITNQNEKDLSDPRNSTLRSSNRLVVDDSDDPPQQQASSKCCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3L4</accession>
    <name>T1H3L4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M13 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3L4"/>
    <dbReference type="STRING" id="36166.T1H3L4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010843-RA">
        <property type="protein sequence ID" value="MESCA010843-PA"/>
        <property type="gene ID" value="MESCA010843"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1020442_0_0_1"/>
    <dbReference type="OMA" id="RAYEMNE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF231">
        <property type="entry name" value="LD31822P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577344" description="Peptidase M13 N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="4">
        <location>
            <begin position="44"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010843-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="273" mass="31694" checksum="6BDD2D1A9AFACF41" modified="2013-10-16" version="1">MLGTFAVLFCVFVPSLYAKPTESPNTSTRYLEDILSYSNRNVDPCENFYEYACGNWKPSSFDSKKERNSLLMSSLNHVIKTINDFLVGNNADLDDVYPYQLGELRKYYKVCMKATAETPKKSDKYLNLLGEIGGFPALDPNWKPDDFDWVAMASHLNVYGCNNLLTEVVYPMFPFPFSFQKMELGFYVALNTRTFQNLSDIAYQVNSEEMLKILRLYGVDDEKSQIIIKDILQFIKRIMKFNIAMSEFYYADNFQEFIDDTKFESLDEVTKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H418</accession>
    <name>T1H418_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02091853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H418"/>
    <dbReference type="STRING" id="36166.T1H418"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011017-RA">
        <property type="protein sequence ID" value="MESCA011017-PA"/>
        <property type="gene ID" value="MESCA011017"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004416_5_2_1"/>
    <dbReference type="OMA" id="ANCILRI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053:SF25">
        <property type="entry name" value="TRANSPOSASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011017-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="330" mass="38126" checksum="39EF3EE8FD52A91B" modified="2013-10-16" version="1">MVELLKKKGINYPDVLTLNVNIDGLPLYKSSKGVFWPILGKFSEIKCMKPPRLFLNDFIDEMKSLANTSFLNTKIYPGMFIMDAPACAFVKQIVGHNAKKACGRCEVVGEFHGRMCYPQISNKSRTDEEFRNRVDKEHHKTYSNDDSEEAGTKSPLEDIEDVDMIDLAQMTKPTEFHRAIRSLEYLSFYKGTEFRNFLLYHGLVALKANVDDEIYENFKDLHCAITICLTNKHRKYVPAAKLIFEKYFRDFKKIYGKCMVSYNIHQTLHLADYVIKYGPLENYSAFEYESKLGIIGNLICSGNNPLEQGANRIIEHLELDMEELLHNQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4B9</accession>
    <name>T1H4B9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4B9"/>
    <dbReference type="STRING" id="36166.T1H4B9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011128-RA">
        <property type="protein sequence ID" value="MESCA011128-PA"/>
        <property type="gene ID" value="MESCA011128"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1242803_0_0_1"/>
    <dbReference type="OMA" id="DMWANIP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042477">
        <property type="entry name" value="HMGXB4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584:SF1">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="119"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="119"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011128-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="25402" checksum="A916603D921D40BB" modified="2013-10-16" version="1">MEKPGGDKDSQFDVTGISRSGRVRKKSYKLIDFEPDHNKSKRPLKGGVKVGRGRPRKNPLPSKDDKSDDEEDMFMSTETDDVEGNDDYSESSDEMSDDFENSNESSNVVYGKSYRKDKNKSRFTAYMLWSNEARKKIVANEPNLDFPSISRKMGDMWANIPKHEKNIWRRKAQNLGIKQKRTILKEVFNPSPASFKSHSASVLNRQKSRTKSLSIQSSATSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4D1</accession>
    <name>T1H4D1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4D1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011140-RA">
        <property type="protein sequence ID" value="MESCA011140-PA"/>
        <property type="gene ID" value="MESCA011140"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592529_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011140-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9131" checksum="7EBE65DC929DBAF0" modified="2013-10-16" version="1">MVQEVKRAVQDIEAVFGVYKDRMSHKPGLRPIISNINIPSSRIAKFLVSEFSSLKPPQGFYIKNSFEIADEIFGMQIQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4I2</accession>
    <name>T1H4I2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4I2"/>
    <dbReference type="STRING" id="36166.T1H4I2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011191-RA">
        <property type="protein sequence ID" value="MESCA011191-PA"/>
        <property type="gene ID" value="MESCA011191"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1227464_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011191-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="25595" checksum="72076A777C6A4AFF" modified="2013-10-16" version="1">MYQIRIEAHNVAGSSKSEFAFVTLTKDGKRPLHSSIENGLSRNYLYSNINIIVYIGITFVLIFCICCSFGFGYKFKKRKETKESLDNSHNLETSQRERYYATIHKVALQNSDKIPETSEDISPYATFQLSEVSALAHQSGPPNTLLHSFMYHERAPTEGCSSTPTSALCKTLSSERKNNLYQKITSKNRRRLHSKGLDSEESESDPDQINAGHIETIKPKLLKSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4I6</accession>
    <name>T1H4I6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase family 2 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4I6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011195-RA">
        <property type="protein sequence ID" value="MESCA011195-PA"/>
        <property type="gene ID" value="MESCA011195"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898632_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011195-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15581" checksum="11D17112D25CC6A7" modified="2013-10-16" version="1">MDSTRPVTAIINAFDIENNIDYQFAHYLDVISLNFELKDPSIDINNLAKTGIEWNEKYNKPVIIAKYGKEMYSNLDKFGDSFDIIKDHKALIGEILDSYRNFEEFCKYLTVNGTRFIEEIGSRYISFYPPGDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4U1</accession>
    <name>T1H4U1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4U1"/>
    <dbReference type="STRING" id="36166.T1H4U1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011309-RA">
        <property type="protein sequence ID" value="MESCA011309-PA"/>
        <property type="gene ID" value="MESCA011309"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038415_1_0_1"/>
    <dbReference type="OMA" id="QMHEFRG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000148">
        <property type="entry name" value="alpha-actinin, sarcomeric isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="198"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="239"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011309-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="292" mass="33917" checksum="A9F35CCDA742BADF" modified="2013-10-16" version="1">MEEIQGLIQAHDQFKATLGEATKNSTQSSIWYQHQIPGGLENPYTTLSAHDLTRKWTDVRQLVPQRDQTLANELRKQQNNEMLRRQFAEKANSVGPWIERQMDAVTAIGMGLQGSLEEQMHRLREYEQAVYAYKPHIEELEKIHQAVQESMIFENRYTNYTMETLRVGWEQLLTSINRNINEVENQILTRDSKGITQDQLNEFRSSFNHFDKNRLGRLTPEEFKSCLVSLGYSIGKDRQGEMDFQRILAVVDPNSTGYVHFDAFLDFMTRESTDTDTAEQVIDSFRILASDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4U5</accession>
    <name>T1H4U5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02164234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4U5"/>
    <dbReference type="STRING" id="36166.T1H4U5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011313-RA">
        <property type="protein sequence ID" value="MESCA011313-PA"/>
        <property type="gene ID" value="MESCA011313"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860242_0_0_1"/>
    <dbReference type="OMA" id="QYESSIA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.450.10">
        <property type="entry name" value="Glucose Oxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027424">
        <property type="entry name" value="Glucose_Oxidase_domain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF216">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011313-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15207" checksum="EFF2A2378E0E8A23" modified="2013-10-16" version="1">MKAFLNAGSELGYNITDINGEHGIGFSRVQANIRDGRRCSTSKAFIQPVKHRQNLHISLKSWATKILIDTESKKAQGVEFVKNKKKFSIKIKREVILSAGAIGSPQLLMLSGIGPKSDLNKLNITVLKDAKVGYNLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H596</accession>
    <name>T1H596_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H596"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011471-RA">
        <property type="protein sequence ID" value="MESCA011471-PA"/>
        <property type="gene ID" value="MESCA011471"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173833_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011471-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12348" checksum="30B84827431CAD4C" modified="2013-10-16" version="1">MSSMYNLTLLDAWSCVKSVGRVSEKFQTLRGFRQGYALSSSFFNILINPQIGKRRLVERIENQSHQESLAQSSPFDFDVVKDFIYMGSENVPQNESYAISPADTACAIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5E1</accession>
    <name>T1H5E1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5E1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011518-RA">
        <property type="protein sequence ID" value="MESCA011518-PA"/>
        <property type="gene ID" value="MESCA011518"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898633_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011518-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="14609" checksum="3A077CCAE4F2B1C0" modified="2013-10-16" version="1">MKIKLVKLGFFGPRILGPLSHGSCKKALSFIERISILSSLALKMITVLPFLCLTLFTALQLFVFRSGFVISRADKMSLISSSPGKAIFIFGGLGRSAKYLSWILAPFQTSPMDVIMVLSTSKPSCDSHVITPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5F5</accession>
    <name>T1H5F5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc transporter foi</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02129992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02129993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5F5"/>
    <dbReference type="STRING" id="36166.T1H5F5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011532-RA">
        <property type="protein sequence ID" value="MESCA011532-PA"/>
        <property type="gene ID" value="MESCA011532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_575258_0_0_1"/>
    <dbReference type="OMA" id="KHKYSAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF37">
        <property type="entry name" value="ZINC TRANSPORTER FOI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588737" description="Zinc transporter foi" evidence="8">
        <location>
            <begin position="22"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="54242" checksum="99FD2099B070AA33" modified="2013-10-16" version="1">MARHIMAVCVVCLLCADHVPCKEHLGLGSGPGTANFQLQNTNNLYPTSSSYGQYYDQYVDATNSLPENYDMIPKSRRKSKRHANHEHNDHQQHERSYLHRPEITNNFLKQIFKEYGEENGTMNMTGLQKMLNKLGLYKLIEKDTENNTCASKDEVISRIHSHQSKKQQQQKKPHCSTENTSSLKNSIPCDDDTKPVAKPNNFTLNTFDIFNLCPIFLYQILAPTDLQNHGCINSSILKEIDSHDHHNFHGHDHEDDGYSENMFYVWLYAILSVLACSVLGLVGVSILPCMDSKFYKEVIQFFVALAIGTMSGDALLHLLPHAIAGQTEHNIIFKGLTAMGGMIFFYATEHGLTMVSEWRKSCKKKDKQQNNSARVLRDPAENSMNNSTGDKLCKAKYSSYPYCYDEIAMDTKNDMHQPNANHIHRKIGNNKVENGNTVPAVIGSSEHDLHETAQSLLSENFTPVSDVAQNIPVAIPRRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5X0</accession>
    <name>T1H5X0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5X0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012103-RA">
        <property type="protein sequence ID" value="MESCA012103-PA"/>
        <property type="gene ID" value="MESCA012103"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010718_6_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF57">
        <property type="entry name" value="LD10016P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="360" mass="40984" checksum="998330FF0ED5BA21" modified="2013-10-16" version="1">MSLSDVSKVFNEKSLDEIIKKIGGVRHTSYEFAKTSEKKGDSYLSVDKPISINTIIKSIPDNAARRKIFRSPDFFRNEIHFYENVLPAFEEFQRKRNPKNPFTEYPRCFESCDGENDFIVLENINYLGYISPDRQSYITLEDCVLIIKALGRFHGMSIAFRNQDPVNFEKAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEDPEKIFPISEVRKELTYFGRFGCGMGIESLPMSMIDEEDVADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Z3</accession>
    <name>T1H5Z3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H5Z3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012126-RA">
        <property type="protein sequence ID" value="MESCA012126-PA"/>
        <property type="gene ID" value="MESCA012126"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020515_4_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13761">
        <property type="entry name" value="TGF_beta_BMP5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF310">
        <property type="entry name" value="PROTEIN 60A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="9">
        <location>
            <begin position="174"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012126-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="348" mass="39548" checksum="210A25B758E459A0" modified="2013-10-16" version="1">KSAPQFLLDIYHRINDIENPENNLLKSRSKRDVNDEENFVTDLDKKAADDSDVIMTFLNKNHHVGEGRHEHGRRLWFDTNDVETDNHLLSAEFRIYQNPSIGKWAGTGKEFVISVYKILKIDGGNKQLELLNSVNTTSDYHGWLEVNVTEALHQWTYNKKSNKGLYISAHVVNRPGKEVRLDEVGLVNTRGDDEYQPFMVAFFKGPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDWIVAPDGYAAYYCSGECEFPLNTHMNATNHAIVQTLVHLHQPKKVPKACCVPSSLGLLPVLYHQTEENVTLKKYKNMIVKSCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6A0</accession>
    <name>T1H6A0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6A0"/>
    <dbReference type="STRING" id="36166.T1H6A0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012233-RA">
        <property type="protein sequence ID" value="MESCA012233-PA"/>
        <property type="gene ID" value="MESCA012233"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929425_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF8">
        <property type="entry name" value="FORMIN 3, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012233-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7070" checksum="AC5D7BE67B3BE959" modified="2013-10-16" version="1">MDARIGLDYIVENREYISKLGAALDTNNFTVKKQVFELLSALCAYNLEGYQRAIETLEYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6L5</accession>
    <name>T1H6L5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H6L5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012348-RA">
        <property type="protein sequence ID" value="MESCA012348-PA"/>
        <property type="gene ID" value="MESCA012348"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045887_0_0_1"/>
    <dbReference type="OMA" id="FHIRCRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057475">
        <property type="entry name" value="CUT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056953">
        <property type="entry name" value="CUT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051962">
        <property type="entry name" value="Cuticlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22907">
        <property type="entry name" value="GH04558P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22907:SF54">
        <property type="entry name" value="GH04558P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25301">
        <property type="entry name" value="CUT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25057">
        <property type="entry name" value="CUT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="10">
        <location>
            <begin position="7"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="38948" checksum="04EA815EC9C2C580" modified="2013-10-16" version="1">EPTVRIKCLPGMLISIKDAPVSHESGSFSGMIYPKGLSKNSTCLSEYRDHTGTLKYKLPLRSCNTMPQETEDGGIEFFNTIVLQPHLKLVTDMGRGYHVRCRYKSREAAAIKENYHHNHKSDDGKKPXAGVEQRNRRNYGRSLGKLSSSNNGSEDDEENSSNALVDLTDDVPMPGCHMKIFNEDEKIAENVKIGDSLTLVINIDKQDIYGLHVTDCIVRDGLGWGEQKLVGDDGCPMDNEIMGPFVYNNDRLSANVTFPAHKFPYTASVYYQCNVRLCALQDPDCQKQTEDAICVSQRTFAIAIAIAGLILMLAVVAAVLCIMARRRTKTVSHSGSSIYSGPYTNTAFSHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6N5</accession>
    <name>T1H6N5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6N5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012368-RA">
        <property type="protein sequence ID" value="MESCA012368-PA"/>
        <property type="gene ID" value="MESCA012368"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369289_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="29"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012368-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="22619" checksum="692A7A7C7C12F36D" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDTLIWKTSSTTIAQFANRMPLLHNQRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDGEEGPWDQFPKIYTKNNLTTYQVSGLTPFSVYSFRILAVNKLGVSPPSKESYYIFTLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H764</accession>
    <name>T1H764_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H764"/>
    <dbReference type="STRING" id="36166.T1H764"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012602-RA">
        <property type="protein sequence ID" value="MESCA012602-PA"/>
        <property type="gene ID" value="MESCA012602"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051989_2_0_1"/>
    <dbReference type="OMA" id="AWNQYFA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01820">
        <property type="entry name" value="PAF_acetylesterase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11852">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11852:SF0">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE IB SUBUNIT BETA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="39"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012602-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="225" mass="25723" checksum="FE03FD8B034737E4" modified="2013-10-16" version="1">MNPCSIPTPANDIDGDNRWISIHKRFLSECLEKDPDVIFLGDCILENLVHTETWNQYFAPLHCLNFSIRNDRTENILWRVVNGELDNVNSKIVVLHVGTNNVDNTPEEVAEGIFEIVKKIREKLPETYIVLPTILPRGQQVNKLREQNSKVNNLIKEKCIGMNRVQIVSIGKGLIQPDGTISHHDMFDYLHLTNTGSKKVFEPVFDLLTQILNENEPEKDLTPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-11-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7F2</accession>
    <name>T1H7F2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7F2"/>
    <dbReference type="STRING" id="36166.T1H7F2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012689-RA">
        <property type="protein sequence ID" value="MESCA012689-PA"/>
        <property type="gene ID" value="MESCA012689"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127869_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032053">
        <property type="entry name" value="Ribosomal_mS34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16053">
        <property type="entry name" value="MRP-S34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012689-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="13808" checksum="4BB5DF3CEB2F0C40" modified="2013-10-16" version="1">ENRKVKVTVERTFRGVTSPKPVVIFSSSYKPDFRLIPKDEEAEYCKVLNNRKPLAPEIDLPPLLREFVCDETKEANPKMKLHFKTNVNKLSRVAKEGEKPTIEIPIGLGTPLSPKLYENCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7H2</accession>
    <name>T1H7H2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H7H2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012709-RA">
        <property type="protein sequence ID" value="MESCA012709-PA"/>
        <property type="gene ID" value="MESCA012709"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058671_3_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012709-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="25283" checksum="80A8E4CDD9B90E2F" modified="2013-10-16" version="1">PPSGGYAPEPGFIQPPPAGYNTYDNAIPPKNFSFDDQGIRKAFLRKVYSILMXQLIITFGIVSIFAFIDGVRDFAQNNMWILWVAFAVTLVTMICMACCENVRRTTPMNFIFLFLFTLAEAFMLGILSALYAPEAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVIFMIFGLVAMFWRGNTIRLVYSSIGAFLFSVYLIYDTQLMLGGNHKYSISPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA42</accession>
    <name>T1GA42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCDC93 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02098019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA42"/>
    <dbReference type="STRING" id="36166.T1GA42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000087-RA">
        <property type="protein sequence ID" value="MESCA000087-PA"/>
        <property type="gene ID" value="MESCA000087"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346177_0_0_1"/>
    <dbReference type="OMA" id="CIECCEY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039116">
        <property type="entry name" value="CCDC93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048747">
        <property type="entry name" value="CCDC93_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441">
        <property type="entry name" value="FIDIPIDINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21673">
        <property type="entry name" value="CCDC93_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC93 N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000087-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="204" mass="23829" checksum="A01FE14BE4784B2A" modified="2013-10-16" version="1">ERREDEEQNLKQQEIFDILVAAGYFRARIKGLSAFDKIVGGMTWCIECCEYGVDVDLLFHENLTIGQKISLTEKIVTVLPQMKCPYLLEPHQIQGLDFISIHPVIQWLVKKSVENRAERAENLKKYAETQFNAHFQFCSDKELSEKAIKDQEEINEKRKSEFPKRVYRRKDYGNEDEFTKVRITLLEYGNEGKIVGKDSVMSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA44</accession>
    <name>T1GA44_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bestrophin homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Forms chloride channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA44"/>
    <dbReference type="STRING" id="36166.T1GA44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000089-RA">
        <property type="protein sequence ID" value="MESCA000089-PA"/>
        <property type="gene ID" value="MESCA000089"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018069_0_1_1"/>
    <dbReference type="OMA" id="WSVTHVL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000615">
        <property type="entry name" value="Bestrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736">
        <property type="entry name" value="BESTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736:SF11">
        <property type="entry name" value="BESTROPHIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0868">Chloride</keyword>
    <keyword evidence="6" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000089-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="28806" checksum="DF05BE458A106AAC" modified="2013-10-16" version="1">MYRFGLPEPQKRIFEKIRTYTSASVDYMPSMLTFLLGFYVNLVVTRWWTQYKLLPWPDNLAMIISYAIPNSNGETGRLMRRNIMRYMVLAYVITLQRISLRVKRRFPTPQHLVDAGLMMESERKIFDTMNAKSPMSKYWLPLVWGTNIINRARKDNVITSDQIIQSILSELKDIRTRLGGLIGFDTVCVPLVYTQVASIACYTFFLSLLIGRQMIPTDEHATKTRDDERFPDLFFPLFAFLQYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA61</accession>
    <name>T1GA61_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02142529">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142530">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA61"/>
    <dbReference type="STRING" id="36166.T1GA61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000108-RA">
        <property type="protein sequence ID" value="MESCA000108-PA"/>
        <property type="gene ID" value="MESCA000108"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280534_0_0_1"/>
    <dbReference type="OMA" id="NCDIHER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050969">
        <property type="entry name" value="Dev_Signal_Modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949:SF56">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7, 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="4"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000108-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="23961" checksum="AF07AB2EA167243A" modified="2013-10-16" version="1">IATCNPPCQNNGICLEPGLCKCPDNYQGPLCQHKKVVCPSKPPVPKNSKVSCRDTNCNVECIKGYKFPDGSGITNIGCVDGQWQHTKTGQSVVPDCEPICDPPCQNSGTCVGFNVCQCSQKYKGPRCEYDYKICNITKAGFNGNFRCSYDSEQAKCTINCPETINFDIAPATEYTCKYSEGVFKPSPMPKCVYRKFNLFHYNFTKQYISCSVHVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAE2</accession>
    <name>T1GAE2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GAE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000197-RA">
        <property type="protein sequence ID" value="MESCA000197-PA"/>
        <property type="gene ID" value="MESCA000197"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712872_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF37">
        <property type="entry name" value="FIRE DANCER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000197-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="388" mass="42841" checksum="7DDBDD16BFE116E4" modified="2013-10-16" version="1">VAICDNLELPFRDDSFDAVISLAVVHHFATTERRILALKELSRILRVGGRVRITGCSNSLATTKEPKLHNCSDEEDDDDFLPPYHAYTEDSTNSSRSAGDGDSSSLSSSSPGESCYSFVRKAIQKLAGNRRHPWFLDSWNSKDTQKDSSLDIEDAKDLPIELRRLEDFEDLVEPPIAAAALKSRSLGCILNNNHPPSPRQIVRSRSSVPSLSGPTTSSHDNKYQESIATTTSSSSTTNNSSTTTSYGGSLSRRPKLIKQKQSLCDEDYQLPENPFYVLDSSNSSIENSKQSNSESPSNSKLFLRKQSSLNEELMAANRLREKERVRKRIQKQTSLNEAFLYRSVFGRKLQVIKEGFASKIKSSTGSLERMTKIGGGGGGGXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAF7</accession>
    <name>T1GAF7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02200060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAF7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000213-RA">
        <property type="protein sequence ID" value="MESCA000213-PA"/>
        <property type="gene ID" value="MESCA000213"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906692_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000213-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6967" checksum="2F01B46DBB49145F" modified="2013-10-16" version="1">MQVIIFNDDFKKDIPLCDEIRTDGGTQFQGNCQHIPSSPHYSQSNGEAESAVKIAKMIIERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAU5</accession>
    <name>T1GAU5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000358-RA">
        <property type="protein sequence ID" value="MESCA000358-PA"/>
        <property type="gene ID" value="MESCA000358"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2998822_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054721">
        <property type="entry name" value="GEO12453p1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22861">
        <property type="entry name" value="GEO12453p1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577170" evidence="1">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000358-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="57" mass="5817" checksum="4B45B9E660E66ABD" modified="2013-10-16" version="1">MFKFTVALFALVAFAAAKPGLVTPLAYTAEVVAAPAVATATAVNSSPETIMASQLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB58</accession>
    <name>T1GB58_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynein heavy chain region D6 P-loop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB58"/>
    <dbReference type="STRING" id="36166.T1GB58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000479-RA">
        <property type="protein sequence ID" value="MESCA000479-PA"/>
        <property type="gene ID" value="MESCA000479"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1158884_0_0_1"/>
    <dbReference type="OMA" id="ISWMGEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="1">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="2">
        <location>
            <begin position="204"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000479-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27567" checksum="E72E7BAC0C2326FC" modified="2013-10-16" version="1">MLLNNYEIILSWVEIYGLLLLQTFITNNMDASFVEPPPFDLHSSFLDSSPTTPLVFLLSAGSDPMASLFMYAKTRNMYDKTKTISLGQGQGPRAERMILDGVKWGHWVVLQNCHVAISWMGELERICNDSSIFKDSHPDYRLWCTSYPSSVFPVSVLQNSVKMTNEPPKGLKMNMMRSFNSDPLVRDKFFSNAFSKDPIKAKNWRRGVFALVFFHAVIQERREFGPLGWNIPYEFNESDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB73</accession>
    <name>T1GB73_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB73"/>
    <dbReference type="STRING" id="36166.T1GB73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000495-RA">
        <property type="protein sequence ID" value="MESCA000495-PA"/>
        <property type="gene ID" value="MESCA000495"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031685_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000495-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="29775" checksum="0D86D5DC77859958" modified="2013-10-16" version="1">MAATPCGSPMYMAPEVIMSLQYDAKADLWSLGTIVYQCLTGKAPFQAQTPNELKAFTLPEIGTTGGLQSLLEADGQDLPFHVPELSEDTLMDKEHNETLSKLNFVLALTDCIQEVADSRCAPLSAAILSSNDQNIPPHLPENCKRIERLVLLIRALQLLSSGLDLAAQKLREGSLNSSSSNVKNALLTMNSKYKSILYESKRLNGTGLLQKASTSNITADRILYEYATDMCQSAALDELLMNTKNCFELLNNQELRICSKLYRRPNPKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCC5</accession>
    <name>T1GCC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="T1GCC5"/>
    <dbReference type="STRING" id="36166.T1GCC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000935-RA">
        <property type="protein sequence ID" value="MESCA000935-PA"/>
        <property type="gene ID" value="MESCA000935"/>
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    <dbReference type="HOGENOM" id="CLU_055356_3_1_1"/>
    <dbReference type="OMA" id="ESSISYW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="AD-003 - RELATED"/>
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        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
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        <property type="entry name" value="Methyltransf_PK"/>
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        <property type="entry name" value="DUF858_MeTrfase_lik"/>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    <keyword evidence="4 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000935-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="188" mass="21330" checksum="1D12D1884B704CE7" modified="2013-10-16" version="1">MSEIKVPETDIPKMEDKETNQNSESTFYTDAKEYWSNIPATLDGMLGGFGYISSLDLNFSERYLRDILKSSQAPQKLRALDCGAGIGRISKNLLMKHFETVDLVEQDTKFSEQAKVNCTGSEESAKRLGTIFNIGLQKFTPEPKSYDLIWSQWVLGHLTDEDLLQFFQRIKLGLKENGVFKMKQISSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2021-06-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCC8</accession>
    <name>T1GCC8_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="MESCA000938"/>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000938-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="14" mass="1762" checksum="25ACBE0727E160D7" modified="2013-10-16" version="1">MVNYKSPFIKMLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCK4</accession>
    <name>T1GCK4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MESCA001019"/>
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    </dbReference>
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        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052830">
        <property type="entry name" value="RCC1_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
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        <property type="entry name" value="RCC1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46849:SF1">
        <property type="entry name" value="RCC1 DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00415">
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    </dbReference>
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        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
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        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="74"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="126"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001019-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="306" mass="33345" checksum="D62B6BBFA0546889" modified="2013-10-16" version="1">LRKRNIFGEIQTNGFPVDEDSIEHISCGSIFSVAVTRNNNVYNIPTKIFEFPKDERITQLASGFEHALALNANGDVYSWGEGLRGQLGDDSMKMRLVPAKVEPLAGIKIVSISAGGWHSAAVSSFGDVYLWGFNSHGQLGISLYKNSSNSENLIKNPSVYSIPQLVDLVCSCKKQETQQTTEAASADTESESTSQSKPEEEPSTISDSTEKIEDTKETTDSGMPLAENTNADSKETTDSEMPLAESSSTENRENIEENMENTSKDSNIQDAQSSKESQSNCNVIKAFCGSRHTIVQMECGKLFSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD67</accession>
    <name>T1GD67_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Histone-lysine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GD67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001248-RA">
        <property type="protein sequence ID" value="MESCA001248-PA"/>
        <property type="gene ID" value="MESCA001248"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_253279_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19174">
        <property type="entry name" value="SET_ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AWS" evidence="15">
        <location>
            <begin position="894"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="13">
        <location>
            <begin position="943"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="14">
        <location>
            <begin position="1067"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="116"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="339"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="393"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="443"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="590"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="744"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="591"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="621"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001248-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1418" mass="160242" checksum="CEEC1A12B82FC074" modified="2013-10-16" version="1">EEESECDDRENVLLNNKNINSKKRFSKAKVLSSPPPITLTLKKTCDSTFLGESSSSNVHDNNSYLSSSDNELPALVNAAIKCVESDSESNSETTPSKMTQQYTSSLLQDYMEKTQLLAQEPSPPNKDKKLKDVVKRKRGRPRKNTKRTLYPPPRSKKVSSSQHSVLSTSSLSTSIKNKLKEETVTLDPIWRKIDVNLKFRRPSLSGYKSDGGGVGTCSKILAAKSGYVSDYGNVRTHSKHGNREKDKDCSGYKSDASFKSRCSSVRSCSKVKKYRRKRDRTRSMLNNASSAVDQDQILQLAGLSLGQSSEESNEYLNPSTSSKKLEIDRFVKTGEYFGKSKSPSAAEDSNKIEKTSKTEPPNGSVLGNIADLDLSRKIKFRRSSAASYCSSYYSGTSRCHSRRKRRKSGLYRNSTGSYKNDPKLNSDIEAIINSFPKSLKKGRKVINDNDNTDSGNTPSTSGNSSKRRHKKTTSSSPDDHKLPLKKRHYLLTPGEKSDASMLLLKTIESSEAASTSKKRHRLDSTPQSGTESLPGIFQQSLELQIQIPNKLTDSIITKAEVESPLEPMSYPSPPKTERIESLLIRSNAHFMNKRKRKKINRTGFPTAKKKKPKPKKEDSISAGSVRSDSLTPISSRGRKIPSPNQNIQVKPFNEKRANLEKVLETMKGRKFFDKATKSPPVNLTYMVTGRGGRHRKTIVPNDDSVDNMSLAKRFRVTKDRDTNFKEPKRDSLPVTAVEKIKARTREKTKTPEISPQSSIIEKPITRRPGRPPKVSQEIKEHLEQEPLPIQEAIATDNESIFSDISIAGLFSDYYKVSQRKKSTIEEVKPNDNILPPPPYCERYFRRTVQDFKLPYDIWHAHMNSKLPTKVASWNFKKLRTNVYAPDCRPNTANIDHPTCNCKPDVGCGDDCLNKMVYTECYPSTCPNKEKCKNQKIQRHEIAPGVERFMTANKGYGVKTTRKIEKGTYILEYVGEVVNEKVFKERMNTIYLHDKHHYCLHLDGGLVIDGHRMGSDCRFVNHSCQPNCEMQKWSVNGLSRMALFASRIIQPGEELTYDYNFSLFNPAEGQPCRCETPMCRGVIGGKSQRIKPIDMSHKKIDFEKESSSQSPTKTTNKMKDVINSHLQPLTEKEKKILKTKKCFLRRNLEKVQRLKGKQTPSPVPIIIEQNKNDNRPMTPSTLAVQISAIRTPRNMKTRCLTQADPEVERMAKVAVSLKDICSSLEAIKDVKTDKPLISRILVGNKKKGKPITTLDFNTIQRNIEKGYYKEALCFDDDMRRLFAVAKDMASENQDKSDILDNLLKIYDAEWKKHAGDFGDPNGFIFAPEEETNEDIIGCICGLYNDEGLMIQCNKCLIWQHSECTKADINADYMCQKCDPHSKIDLEIELNETSEEGFKYYLALLRGHKGQCDTCQKAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDG5</accession>
    <name>T1GDG5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl transferase family 25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02182327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDG5"/>
    <dbReference type="STRING" id="36166.T1GDG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001351-RA">
        <property type="protein sequence ID" value="MESCA001351-PA"/>
        <property type="gene ID" value="MESCA001351"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_988924_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050211">
        <property type="term" value="F:procollagen galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06532">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF53">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="4">
        <location>
            <begin position="8"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001351-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="33394" checksum="1EB8FA251E422158" modified="2013-10-16" version="1">MGIKYLWEYEDIYYPRQMKTGEVGCFLSHYKIWEQMIERGEKEVLVLEDDVKFEPNFITNAMHILKQIREVPNWDFLYFSREDVQFSKEIIVPGTENIVYAEYSYWTVGYVITREGAKKLINSKPLEYLLPVDEYLPIMIRSDHNEDDSIAITKLWLKRVEPLYHNVDFAFDESQKTFYFSKNRVHWSNDRFAHIISLKEEAFQFARRSSTDFVFFCDVDVLLTNPKTLDILIKLNLPIVSPMLISANHLYSNFWALEEEYTIIMSQGVGEFKVSMVHSAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE13</accession>
    <name>T1GE13_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02135612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02135613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE13"/>
    <dbReference type="STRING" id="36166.T1GE13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001570-RA">
        <property type="protein sequence ID" value="MESCA001570-PA"/>
        <property type="gene ID" value="MESCA001570"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1549393_0_0_1"/>
    <dbReference type="OMA" id="VRECECL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001570-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="19944" checksum="FC4F9AB9D53994AA" modified="2013-10-16" version="1">MPRNQSIPICGGNLMECYQKAEIELSEDYSQELSCKCLPSCTTISYDIETSQTKYYKYPNYLVKEKDFLNKTIFSPIDVSSLHISFKEKDFIALRRSELYGFSDFFANIGGLFGLFLGIIDLLLWCPSPLGSTIAFFVAFSMRFNRLRGACFSSRFIYHLHMIRHLKALFQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEG6</accession>
    <name>T1GEG6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nose resistant-to-fluoxetine protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001728-RA">
        <property type="protein sequence ID" value="MESCA001728-PA"/>
        <property type="gene ID" value="MESCA001728"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1391663_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22147" checksum="A27AB9C2DBB1F095" modified="2013-10-16" version="1">MSEILIQISNLASSAYDSSGHFRGQILYGNNKWLGQKRFCQEISTNTSLKFAYSISSISVNITNVIKYSSILEIGECLPNVCSSKDINLILKADPQSNYISSLQLTQTFSILEVRTRETVKKWIDYRFKIFVSLTIFVFILVNLATIYVAFKENNELKPKTTNLEMQSTKDESNVVAGSKIKETVSKKSSKTFLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEG8</accession>
    <name>T1GEG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02018939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEG8"/>
    <dbReference type="STRING" id="36166.T1GEG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001730-RA">
        <property type="protein sequence ID" value="MESCA001730-PA"/>
        <property type="gene ID" value="MESCA001730"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="OMA" id="HENYGGG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF95">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577307" description="Peptidase S1 domain-containing protein" evidence="7">
        <location>
            <begin position="17"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="31"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001730-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="26150" checksum="DDBE4824B81ACC3C" modified="2013-10-16" version="1">MKVVFVLLALAVGAYSLKLPQLKAAFSKGDIIDGVEAQRGEAPFIVSLQYLSSHFCAGSVINERWVLTAAHCLIYNSFSVVAGLHVRSDKTGVQTRKVANNKQYIIHEKYGGGVGPNDIGLIYLTEPLDLNALKAGGAPVSAIKLPSGQYAAAGDGTLYGWGKNRAGSSPQTLHKLETRGDNKYEGACNGDSGGPLVKYTPFGVEQVGIVSWGYTPCHTSTYPSVYTSVASFKDWINTNIANYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GET4</accession>
    <name>T1GET4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02059811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059813">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02059816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GET4"/>
    <dbReference type="STRING" id="36166.T1GET4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001855-RA">
        <property type="protein sequence ID" value="MESCA001855-PA"/>
        <property type="gene ID" value="MESCA001855"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_394997_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Adaptor protein ClpS core" evidence="3">
        <location>
            <begin position="551"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001855-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="698" mass="79823" checksum="0F2C88910AF7D9A3" modified="2013-10-16" version="1">MHTFYYESIDKFVENNVLRFKKNPQSLNICRRASCILSDVRYILNFKPETWTDELRDGFMDGCKVLVKLLGVMQGMESVTRQTGQHMDYEPEWESAFNLHIKLAGVISLILDWCATDREVLTKLYALVAKKTVEKNIFLKKIDISEKKLAGHVARCNIYDVASKPVSIHLPVSRMFAGLYVLLGKFGLCYDNAPFTSTLNINRLSEEIIEPILCTQTMCAQVQAGMWRRNGYSLVGGGSAQSKFMSSNAEMFMDCEEEAQYSDNLDIEGAAAYNSTACLGFHRRFQQPEDPKFKCILCFEDAILHKDKPVLVYPAFIQKSKIIYPLAENKAVDNIEDCAPPSPHTSTCGHVMHGICWKEYFDNEVLKENRRLNRTRPPKNFQAEKREFLCPYCRCLSNAVLPLIPPQQNESNQFEENETMSFDNWLSVMHDFLNELYILEQKTVENAGVAFANTPIECEFPKLSKILEHCGLEVASMLTLCHPKDKLESALSLSKGKNSSSESKIITSRMKERCEIAFRAILTYSVKFLEIEADASLECLDNDNQSDESDEGQYCTVLYNDEGHTFEQVIQTLTRIIKCQQKDAMEIVTSIDRDGRAVAKCGTFEQCRQLQEQIEKQPMQYTSSIPRMQPLKVTVLHKRAVSCQQFALQLLTWFQDFLTRHSAFRAVFAKVIGDKTARSYNIAHILEYDSKLWKNARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF82</accession>
    <name>T1GF82_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002014-RA">
        <property type="protein sequence ID" value="MESCA002014-PA"/>
        <property type="gene ID" value="MESCA002014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087569_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13133" checksum="ED841E24AB87B19A" modified="2013-10-16" version="1">MDRVIIGKLFTAFSEIVIEVIKFTDLEESTKNILSDNIASICDIYALFYILKCVFEQIRNCVGQGNFHGACNIAHPILIKNKPKTDSKSETTQSQIKHVLCDIRHGTNILDSTPTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFB1</accession>
    <name>T1GFB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MOB kinase activator-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFB1"/>
    <dbReference type="STRING" id="36166.T1GFB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002048-RA">
        <property type="protein sequence ID" value="MESCA002048-PA"/>
        <property type="gene ID" value="MESCA002048"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038321_3_2_1"/>
    <dbReference type="OMA" id="VDNEQMF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000001">
        <property type="entry name" value="MOB kinase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002048-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25400" checksum="3B18A49A581A2862" modified="2013-10-16" version="1">MEFLFGSRSNKTFKPKKNIPEGTHQYDLMKHAAATLGSGNLRSAVALPDGEDVNEWVAVNTVDFFNQINMLYGTITEFCTEESCVIMSAGPKYEYHWADGQTVKKPIKCSAPKYIDYLMTWVQDQLDDETLFPSKIGVPFPKNFQTIAKTILKRLFRVYAHIYHQHFGEVVQLGEEAHLNTSFKHFIFFVQEFQLIERRELAPLQELIDKLTDIRKQADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG18</accession>
    <name>T1GG18_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG18"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002323-RA">
        <property type="protein sequence ID" value="MESCA002323-PA"/>
        <property type="gene ID" value="MESCA002323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770189_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16639" checksum="40D771C2A10004BE" modified="2013-10-16" version="1">MILDLNYEFPLTSTKDKRAALNFIGSRTFGVLAEDHAKLIKQEIENSRQNEIHLIKLLKSQTSFIESTLTSLKLAEKRFGNKFQQTNTILDKEFAPIENRNWYLAGELLGSGGFGSVSEFIGHSAILAERSLTCGEVCGNRREMVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG41</accession>
    <name>T1GG41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG41"/>
    <dbReference type="STRING" id="36166.T1GG41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002347-RA">
        <property type="protein sequence ID" value="MESCA002347-PA"/>
        <property type="gene ID" value="MESCA002347"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188617_0_0_1"/>
    <dbReference type="OMA" id="YMAPARV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002347-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="9598" checksum="27ABF2D9E2693ED5" modified="2013-10-16" version="1">MSSGKGLELIKEIMKRVHKGGFDRVIKNVEILSCADGKCSAKFNVSDEQVNMGGGLHGGFTATLVDNITTYALMSKGTHPGVSVDLHVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGA0</accession>
    <name>T1GGA0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zasp-like motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGA0"/>
    <dbReference type="STRING" id="36166.T1GGA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002413-RA">
        <property type="protein sequence ID" value="MESCA002413-PA"/>
        <property type="gene ID" value="MESCA002413"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913896_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002413-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="35416" checksum="DAAF42C608F3C527" modified="2013-10-16" version="1">PYRSTPLVLPGPKVKKDAPTTESYLRHYPNPKVRAHPGQHDYHDVLMKQRVADTMLHKVVGAEADSGRSNLMQIKHIFKNIFKIQKTCQCNEKLKSHNFHRFIFPGTKTQNSGATKIEATTGKTRKQKPTKLQRMSAFLIFWRTWNIFQQSVFPICLYFPYIYFSVCSPFFSFQMSMYKKTVVYDPSKSDTFRAIQEEGYGNEEYIQEVTTPVQPKVFSPSSKMVPGKKLPSLSPRPSIVDLWPPVMASLFLDYILAHFSIFQILSRSGRVDQPHFLKRVDYSHENAHFSIFCMFIYNVKCVCLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGL4</accession>
    <name>T1GGL4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGL4"/>
    <dbReference type="STRING" id="36166.T1GGL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002536-RA">
        <property type="protein sequence ID" value="MESCA002536-PA"/>
        <property type="gene ID" value="MESCA002536"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134790_0_0_1"/>
    <dbReference type="OMA" id="HRINAND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000203">
        <property type="entry name" value="Cyclin-dependent kinase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF233">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002536-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="23584" checksum="142C682E02210ADC" modified="2013-10-16" version="1">ADFGLARAFSVPKADSKNRYTNRVVTLWYRPPELLLGDRNYGPPVDMWGAGCIMAEMWTRSPIMQGGTEQQQLQYISQLCGSFTPEVWPGVDELDLYKTIELPKGHKRKVKDRLKPYVRDEHGCDLLDKLLTLDPKKRIDADSALNHDFFWCDPMPCDLSKMLSQHLQSMFEYLAPPRRSMGRYQQQNQIVKPQDNTFSDRVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGN3</accession>
    <name>T1GGN3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002556-RA">
        <property type="protein sequence ID" value="MESCA002556-PA"/>
        <property type="gene ID" value="MESCA002556"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329228_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002556-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23016" checksum="CD355D38DB534A33" modified="2013-10-16" version="1">KFFLVSNALVCWTCVDDDCLLPKVNWTYILCDQDYVNTSHHQISSIYGLEPSPPKNVKNFECVEIDTKLQFTNSSEKFASFKGCVENKFNISSFSPNSTIYLNEERNEFRTCDMEMCNKLEISRPIPPLTTPSTNTTTTPVVSTPAINNTTTPVVSTPASNITTSVASTPPSPSTPIPPPALKCYICTDEECSKTPKETIVPEKKWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHJ3</accession>
    <name>T1GHJ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394002">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHJ3"/>
    <dbReference type="STRING" id="36166.T1GHJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002897-RA">
        <property type="protein sequence ID" value="MESCA002897-PA"/>
        <property type="gene ID" value="MESCA002897"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060791_1_0_1"/>
    <dbReference type="OMA" id="KDMDPRW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002897-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="249" mass="28127" checksum="ADEA756F071A0533" modified="2013-10-16" version="1">MTELVIDPNIRVWVFLPIVLITFLVGIIRHYVSVLISSKKKTELTQVQDSQAMIRARLLRENGKFLSPQSFAMRKNFFNNEETGYFKTQNRPPVAPNSTAMLSDMVKGNFVNVLPMVVIGGWINWMFSGFVTTKVPFPLTLRFKPMLQRGVELASLDAAWVSSASWYFLNVFGLRSIYSLVLGENNQADQTQATQDQMTGAAMAMPQDPKAAFKAEWEALEVTEYKNALKNVENDLLAKISPNSQNHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ27</accession>
    <name>T1GJ27_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ27"/>
    <dbReference type="STRING" id="36166.T1GJ27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003467-RA">
        <property type="protein sequence ID" value="MESCA003467-PA"/>
        <property type="gene ID" value="MESCA003467"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1588350_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF2">
        <property type="entry name" value="IP13792P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003467-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19154" checksum="671DBFA2A44075AD" modified="2013-10-16" version="1">MDNDGPSGMMRPLSRDLHAAINRVANDFNAKRVNIRKMKWSLDISLSAMLTMENIETIYHKTEEGEKPKTICTETVKYFCGCSDSILPSVIFGHLQNFMKIIPNSRHKQLATIIEALKTEFRQLLGTDGILPAAFTQFNVFPSTIWLSFMSFEGMPNWFIDWKSSSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ71</accession>
    <name>T1GJ71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02010469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003515-RA">
        <property type="protein sequence ID" value="MESCA003515-PA"/>
        <property type="gene ID" value="MESCA003515"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1951235_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003515-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14195" checksum="C23ADCBA324ABD19" modified="2013-10-16" version="1">MISSPLANTIETTIAAVPLNSDHASAAASRVVSRGTTSITLSVRFTNNLGHRIDTGLLETSQIAFLLEESRHPLLVFSGDMEDWPLFESSILKSNEAFQYSDLENNMRLVECLKGKAKEALESLLIHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJA8</accession>
    <name>T1GJA8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJA8"/>
    <dbReference type="STRING" id="36166.T1GJA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003553-RA">
        <property type="protein sequence ID" value="MESCA003553-PA"/>
        <property type="gene ID" value="MESCA003553"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028381_0_0_1"/>
    <dbReference type="OMA" id="LNCGMLG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF44">
        <property type="entry name" value="LD35051P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003553-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="317" mass="37012" checksum="86C0350A139C6449" modified="2013-10-16" version="1">TAKLTELQIESGGSPKRNVIVTSWRSGSTFLGDILNSIDGTFYFYEPLMHYGVKRFNESASKEDKADIFNHIKKLLNCEFNNMAPYLNHAKEFPFQFEHNHRLWSDCNGTNPKYCFSPQFLEPYCSQFPFLTMKIVRTSLDIIQDLLSDESLNVRILLLVRDPRPTLHSRWKDNFCVPEKFPLCGKTELVCDQLISDFKTADFLSQRYPNTFMTMRYEDLALSYYEQSGKILSFFGMAFNEDVKQFLDSHTQSSKGNEFSTFRDTKKIALRWTKQLSWERVSKIQIAEQCAEAMNLWGYKIANSEEELRSEEFNPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKC7</accession>
    <name>T1GKC7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">KASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02138834">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GKC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003949-RA">
        <property type="protein sequence ID" value="MESCA003949-PA"/>
        <property type="gene ID" value="MESCA003949"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001444_0_0_1"/>
    <dbReference type="OMA" id="IMNTESS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000186">
        <property type="entry name" value="nesprin-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000195">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000188">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000230">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052403">
        <property type="entry name" value="LINC-complex_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 300 KDA, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535:SF1">
        <property type="entry name" value="NESPRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="365"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1333"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1591"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1782"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1996"/>
            <end position="2030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003949-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2144" mass="246895" checksum="7B1C81A66A33D49A" modified="2013-10-16" version="1">IQVNVQQVTSRFQSIQATTKEIHKKCEQSVADHQAFNDKYKQCSDWIASAQAKYDDCNDLSTVGSRDDLLKKQAAINDIQSQHGAATLLLNSTVELGEKCYPSTAPEGRENIRATLEELQQVFDQLFDNIGITSRKIQDKMAKWSGFDEIADKLKQWLADIENQLPNEIELKTTLDEKRAKLQAYRDILNDINNHQVEVTNITEIAANLPEQTDLVKEQITQIKDKYNKAQKRGQDFVQLMDTHEFIDATHNTIDLWGDLDLEQVSLHTNLGRLKNLQTSMADEFPRVDQVRSLGEKVIPGTIESGQINIRSQIDNTQQEWEGLLSAMSTIIESIENRLQQWSEYEQLRDECLNWVRDCDNKLHAIDLKDSLETKKQQFEELKALQGDVRAKELEIDNVSEKGQQLLKHGGSRISQGTELVSKYQQLSYKVKELNNRWQQYVSTHQDFQNQVGDCVQWMDGIKEKLDYCSDLSAMSQKELEKKLATIQDVILLKDEGSAKIQSLVEQAQAVLANTAPQGHEPVNRQIADLQEQWSAIALRMVDIKSNLDDSITQWSGFLEQVANVQRLEKRSQLDRIKATEEKVRMEKIDVDALKSQAKEMLESGQQSQAAFQAQKTLDKFDELVSKTEKLLSERQDQYRDHRLYKEAYDDLVSWISRAREKFPSLKQSSLSDKLAIEAAVQATENLLNKQAQGELLVEHLLHTGEVVVASTSPQGQEIIRNDIRALRDNFEALFREINQQKDSLESTTLQWRDFKEEYERLMEWLQQIDIIVKNHKLNLQPNLVEKNKQVADMKDVLDRLDKGNADIEKFTKSAAGLLKSHLDNYVQNQLRHLTSMYQVKVNLAKDVYKKVETNRDQHQEFEDNLNKAQGWIKDAWEIIRVCNLIRRREEGQNLVNSAINNGEKVVRNTRSDGRDTINSQIKDLQTEWDRLVKKMSTAKVHLETNLLQWADYSSSYDQLQQWINDREVKLQEACEKKIVKSQRGQPGLSSGLSERKANLRQTNNIVQDIVSFEPMIQSVTSKASDLQQGAPASEISNKYEQLTKQAKDLYEKQKNTIDHYQTLIDAGNDFAQWLRNAKERLSKCSEPTGDKEALASKTHQLKILESEVPEGKKKLEHALKEGEQACQHAEPGDREIIEEEVALLQEEFDNYIDALNKAKEFLEVGIVKWTDYQNQYADALEWLTKTEGLVQSYNKLQDKLFDWQKELDNLNVKAQLLLETCSDTRISNGIMQLTTKYNALLGLAKEVMRRLEMHYQEHQQHNSLYDECEAWIEKTRERLADCQNIPNTLSEVQIKLNTVKNLRQGFEHGQNKLRYLLELKEKVIMNTESSGAAKIQEDTENLKQDFEKLLVDLNDVKQKLNNRAAQLEEIFKLYKLLLEWLDEIEPNVKLADDFLNDLSEKRSALEKYRSIQREINSHSDMVEKINTRLDEDKNLNRDDFKEGLDRFDKIQEITGRNIENLENQVNNHEKYKQALNEIQDWLRKTSIEIEQCSDSHGEKEQVVGRLEKLKTIAETIPDGKDAINQEVKTVQNEWETLQSMLRDSQGNLESCLSMWNNFLNKFTTINKWIEDMSKKVASSSETENKTPEDLTQAKNLLKEVLDEKDNVEELNDQCELLMEQTACSRVRDETIETQANYTKLLTSAQGLVSKIEKSLSDHTEFLNYKNEMDNWINKAKEVLAECTGEGDLNMISHQLETINSLSSRLPEGQHLLSMVQDAYSKAIHVTPEDQQDKLRGLMTKVREDWDALGLEVKQKLVDPNNPKTDGPKAILGKPVDTKGELSEMKTLLERFKILLNEINQRGPQFENLQQEAKQLGSEIDEVNRLQGKWEKLKNDCNDKIKSIESEINEYNSYYQNLQDVEKWLLQVSFQLMAHNSLFISNREQTQEQLSQHENLLDEIQKYQTNIDDLNTKGQAQIRRYEEKTPSIRGTVEEQLKNIQESYNSLLHTSVQIKNRLLESLAKFQEYENTLDSIMKNLEEYEPIITNELDAPAITLEMAQTQLQTAQNLNNKLQAEKSRLAAAVQACEAATASISRPSSPLESAMQAIPERELVVRAKLEDLLDQVQSHLGGLTATVSELEQQQKQRAELQDWIKKQQSSVADWLSRPCKLRPEAAKQELVAMNDLLNAVGDKRSQLMTDMTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKM5</accession>
    <name>T1GKM5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02046904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004052-RA">
        <property type="protein sequence ID" value="MESCA004052-PA"/>
        <property type="gene ID" value="MESCA004052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888315_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7758" checksum="3E6D5010941D7F90" modified="2013-10-16" version="1">MGHTRDGHYLRKLNVTYRSKKTELSFRQQEPMWIKISRGNRKWRKDSHQMELEHNVSSFRKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKV3</accession>
    <name>T1GKV3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKV3"/>
    <dbReference type="STRING" id="36166.T1GKV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004134-RA">
        <property type="protein sequence ID" value="MESCA004134-PA"/>
        <property type="gene ID" value="MESCA004134"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657315_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004134-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8602" checksum="58B01709BBD30846" modified="2013-10-16" version="1">MTSWGNYVVFKLDDYILVYSGCTCNVNRFRHLDLRTGTAGLICFKSCHEAILFILSGNSQKSANRSCHDTTEYVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL75</accession>
    <name>T1GL75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02389838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL75"/>
    <dbReference type="STRING" id="36166.T1GL75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004268-RA">
        <property type="protein sequence ID" value="MESCA004268-PA"/>
        <property type="gene ID" value="MESCA004268"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253089_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF210">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="59"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004268-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11962" checksum="0F4841405EB4CA4B" modified="2013-10-16" version="1">MDFLCCLLPPPQNHFELNSTTAEMGKKNVARNLQYRELEIPSRQSATNLQSSRLQMDNFRLLSVLGRGHFGKVILSQLKSSNQYYAIKALKKETLSRETKWSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL80</accession>
    <name>T1GL80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02011608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004275-RA">
        <property type="protein sequence ID" value="MESCA004275-PA"/>
        <property type="gene ID" value="MESCA004275"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270470_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004275-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="12008" checksum="CE92641EA452954D" modified="2013-10-16" version="1">MKVNFERWNTVCKKDDLHSKLRALTYSNVEFDEECQRSNKTSGEDSWNSKEFVVINIKEDRSVKTIQTVSGDSRDDGEIMSDYEEFDNAREERSEEDEITGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLH6</accession>
    <name>T1GLH6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GLH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004378-RA">
        <property type="protein sequence ID" value="MESCA004378-PA"/>
        <property type="gene ID" value="MESCA004378"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1205977_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004378-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="26334" checksum="8438EC8D98F5F9CC" modified="2013-10-16" version="1">MVLHGCTVYFCCRNRKSAEEAVASIVKEKSSLGKQCKFIHLDLSTLKSVKQFCDELKSQISLINFLILNAGVFALPYTTTPDGLETTFQISHLSNFYLTQELSPLLDNRSRVVVLSSESHRFSNLNFENLDEQKLSPPASKYWSMMAXNVASIRQRFDSGSKMLFYGSESWSLTVAYEQLLAIYELFRKYIHPCIANMIRKNGLRERKETIATSSKVEESRKEQQRTGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMC2</accession>
    <name>T1GMC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 16">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with SCYL1. Interacts with JAGN1. Interacts with TMEM41B. Interacts with SVEP1. Probably interacts with PEX11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Golgi apparatus membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMC2"/>
    <dbReference type="STRING" id="36166.T1GMC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004692-RA">
        <property type="protein sequence ID" value="MESCA004692-PA"/>
        <property type="gene ID" value="MESCA004692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007565_1_0_1"/>
    <dbReference type="OMA" id="EMTYQKQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000010">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="5 17" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="18">
        <location>
            <begin position="342"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="20">
        <location>
            <begin position="610"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="19">
        <location>
            <begin position="821"/>
            <end position="1121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1121" mass="127210" checksum="166BE3AC13B2CC3C" modified="2013-10-16" version="1">MLTNFESKSARVKGLSFHPKRPWILASLHSGVIQLWDYRMHTLLEKFDEHDGPVRGVAFHCQQPLFVSGGDDFKIKVWNYKQRRCIFTLLAHLDYVRTVCFHHEYPWILSASDDQTIRIWNWQSRQCISVLAGHNHYVMCAQFHPTEDQIVSASLDQTSMGYFWIEEEECCSWTWRALEEHLKSGATDLFGQSDAVVKHVLEGHDRGVNWASFHPKLPLIVSGADDRQIKLWRMNEYKAWEVDTCRGHYNNVSCVLFHPRQDLIVSNSEDKSIRVWDMTKRQSLYTFRRESERFWILTAHPTLNLFAAGHDGGMVVFKLERERPAYTVQGNILYYVKDRFLRKLDFTTTKDVVVMQLRAGNKSPAHSISYNQAFNAVLIATRTNNLENSTYDLCTIPSEGDSEQEGGSDSKRSSGITAIWVARNRFAVLDRNSQLVIKNIKNEVTKKIPTNCEEIFFAGTGNLLIRDAEYVTLYEVQRMICCLVADMSLVALLCKHSVTICDRRLQYLCTIQENTRVKSGAWDDSGVFIYTTSNHIKYAITNGDHGIIRTLDVPIYITRVKGNQVFCLDRECSPRVMNIDPTEYRFKLALINRKYDEVLHMVRNARLVGQSIIAYLQQKGYPEVALHFVKDEKTRFGLALECGNIDIALEAAKSLDEKSCWDRLGQAALMQGNHQVVEMCYQRTKNFDKLSFLYLITGNLDKLKKMNKIAEIRKDVSAQYQGALLLGDVQERVNILKNCGQNSLAYLTAATHGLSEQAQQLAETITESGNELPQLNPTATLLKPPVPIQQAESNWPLLRAMSTRAVSNVRQALDMNEPVIDDSAAGEGWGDDADLGLGGDEDDEFKDPVGDVDAVGDGPGWNVEDDDLIVPQEILDKMKTTSISSEGFFAPNKGQSVQQHWSNNSSLVADHVKGGSFETAFRLLNEQIGVVNFKPFKELFMQLYAGSRTSFSAISNIESLTAYPNRNYSETVPKNMHPAIGVRLSDLVQRLQDGYQLTTGGKFTEAIEKFQNILFSIPLLVVENKTEIAEAQQLLKICMEYILGLKLETARKALPKSTLEEQKRLCEMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYKTAASFASRLLELGPRPEVALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2021-06-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMC9</accession>
    <name>T1GMC9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA004700-RA">
        <property type="protein sequence ID" value="MESCA004700-PA"/>
        <property type="gene ID" value="MESCA004700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3436028_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004700-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="13" mass="1391" checksum="5B2C3AE5B08CBB17" modified="2013-10-16" version="1">MFTSSLESTLSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMM0</accession>
    <name>T1GMM0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02052730">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMM0"/>
    <dbReference type="STRING" id="36166.T1GMM0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004796-RA">
        <property type="protein sequence ID" value="MESCA004796-PA"/>
        <property type="gene ID" value="MESCA004796"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078922_0_0_1"/>
    <dbReference type="OMA" id="HQANGRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004796-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13628" checksum="F6BF2FA23696B9AE" modified="2013-10-16" version="1">MRLWQVSNGGVRMSAESRSGRKRVTRLVVVVVVAFASLWFPIQTILLLRSFGWDTTTPFTVMLQIISQILAYTSSCINPLLYAFLSDNFRKAFDKVSIIIFSTLLYSIQYIQFYFLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMR7</accession>
    <name>T1GMR7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GMR7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004847-RA">
        <property type="protein sequence ID" value="MESCA004847-PA"/>
        <property type="gene ID" value="MESCA004847"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418433_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577030" description="Carboxylesterase type B domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="3">
        <location>
            <begin position="26"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="192" mass="21234" checksum="8FC452A20DF9893D" modified="2013-10-16" version="1">MKTIIIVLLKLLMGNHICAMPTDDLAVTLDHGGRLTGKYLESFEGRKIRSFQGIPYAKPPVGDLRFEPPIPFPPWTGTREATKEGNVCPQIDHMANGAEIGNEDCLFLNVYTPPLDIINKTGNLPVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMS4</accession>
    <name>T1GMS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004854-RA">
        <property type="protein sequence ID" value="MESCA004854-PA"/>
        <property type="gene ID" value="MESCA004854"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906658_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004854-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7070" checksum="4600FBCE5DC25602" modified="2013-10-16" version="1">MEKAKAYIKKNELRGGTLSATETPNYPPPSSSAPIHYPSSQQQYYHHYQQNLHNYLESISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMV8</accession>
    <name>T1GMV8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02023732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMV8"/>
    <dbReference type="STRING" id="36166.T1GMV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004892-RA">
        <property type="protein sequence ID" value="MESCA004892-PA"/>
        <property type="gene ID" value="MESCA004892"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_540353_0_0_1"/>
    <dbReference type="OMA" id="ASQCESW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577494" description="SCP domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="65"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="269"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="505" mass="56456" checksum="8531BCB810D9DBCA" modified="2013-10-16" version="1">MVSKIYIIIVLSTALLNIVLCAFTPGGDFCEKLKCAKETHLFCGAKLTPKGVLGNTPKKVELTDDQKKIILDKHNELRNILACGEPKMINLEGNIFPKPAKLPKVIWDDELEWLADLNGKTCAFAHDACIESPKYEKAGQNLGILKSTSPITPEEATVSNIEEYLVTPLDVIDGYSQNVLKPGENEEDVNKIKEAGFKVANSNGHFTSLVRDTTDTIGCVFYECGPIEDMTHSYYLEGCSGGTHAYCGGTLKHTGLLGKTPTKVNLTKAMKQYILDRHNELRNIIACGEPRMINIAKEVFPKAAHMVKFHWSTELEWAANLNGVTCSMSQNCPHTVKFPFGGQNQGYNISSTPKNISRFLDEQIQEFWNEYLNTPIEAVDKYSPKLIKNPIAGEEAMFIKDSKLTKYPSKITKNSRFAVLARDETEYVGCVVYSCGKVKAMEYSYYLVCNYGLSNINNKPIYKKEDSPNSECKNKSTEFCCLCMKSHYKEHGGFCRKSTLVLPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNC0</accession>
    <name>T1GNC0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005067-RA">
        <property type="protein sequence ID" value="MESCA005067-PA"/>
        <property type="gene ID" value="MESCA005067"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834056_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005067-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7411" checksum="EC33FFB5D9C3828F" modified="2013-10-16" version="1">MRSHKSLEITPPYCVPLLRPLLIPSLCYQQIIVGGWGTGNKKVNLLDLTVFPLSGANYCHSHEINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNX7</accession>
    <name>T1GNX7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DEAD-box helicase OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNX7"/>
    <dbReference type="STRING" id="36166.T1GNX7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005290-RA">
        <property type="protein sequence ID" value="MESCA005290-PA"/>
        <property type="gene ID" value="MESCA005290"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050429_0_0_1"/>
    <dbReference type="OMA" id="KMVICAG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box helicase OB fold" evidence="2">
        <location>
            <begin position="93"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005290-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="371" mass="43253" checksum="9F6B7CA7C1C0272B" modified="2013-10-16" version="1">MMMAETTGEESSAERSKRHGEVQMLKSLKRAQKFQEPRKKKFLKREEFTEESFDDQTKDIRDVDFRLSHDSNQLEILMKSCNIQRSFRDLVLLKMVICAGFYPQIAIPDEFNYCKGGTQQFFHTFLKPFVSMHPNSQFSKNFEILKLNESEILDKPRYYTAKNTLSSHHQVLCYQNLLETSRPYLMNSVRMPAAQTLLLFSFSIDTNSSVTNVVCDSWLSLDFPVPEMGREVLLRAVKLRRTWQKLLSKKLDEVEGVSSGDSKKESRRDKQIREDLWDDLIKFTSLNIVYSVKKHLPADLRNLYNQKELPLEKYSINPFAEDFPVSQNVEKGGLQVAENVVYGCLRETNWSEVLTWSFRSKPGINAKRNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP08</accession>
    <name>T1GP08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alkyl transferase</fullName>
            <ecNumber evidence="4">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02166771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP08"/>
    <dbReference type="STRING" id="36166.T1GP08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005322-RA">
        <property type="protein sequence ID" value="MESCA005322-PA"/>
        <property type="gene ID" value="MESCA005322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_4_1"/>
    <dbReference type="OMA" id="TKGQPDP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="225" mass="26403" checksum="3F607E82A995C7F9" modified="2013-10-16" version="1">MKIFLFFRNRRFAHSKNMEKIDGHTKGFEKLAEVLKWSLDLGIKEVTAFAFSIENFRRSDQEINDLMTLATEKFENFVKENVKLKENGVRLTVIGDLNLLPTKLQEVVSRAVSETKENNKLFLNIAFAYTFRNELVSACKNILETNYEGNNDEINDELLINNLQLKSVPDLLFRTSGENRLSDFLMWQLSSSILYFTKILWPQINIRHFFAAIFQYEIIKFKNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP37</accession>
    <name>T1GP37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02091696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091697">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091698">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP37"/>
    <dbReference type="STRING" id="36166.T1GP37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005353-RA">
        <property type="protein sequence ID" value="MESCA005353-PA"/>
        <property type="gene ID" value="MESCA005353"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099695_3_1_1"/>
    <dbReference type="OMA" id="YHNVIAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF304">
        <property type="entry name" value="DNA-BINDING PROTEIN D-ETS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="4">
        <location>
            <begin position="12"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005353-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7989" checksum="180900525DF8E085" modified="2013-10-16" version="1">MIIFHMSGSGQIQLWQFLLELLSDSGNAGCITWEGTNGEFKLTDPDEVARRWGERKSKPNMNYDKLSRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP64</accession>
    <name>T1GP64_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02177289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP64"/>
    <dbReference type="STRING" id="36166.T1GP64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005381-RA">
        <property type="protein sequence ID" value="MESCA005381-PA"/>
        <property type="gene ID" value="MESCA005381"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_3_1_1"/>
    <dbReference type="OMA" id="EVENPEX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF240">
        <property type="entry name" value="LD25378P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005381-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="27553" checksum="8A76D2F3FF24E015" modified="2013-10-16" version="1">MARVSIGDNLVPKESFGEIRDDLAKNESISSSSEGIKFKKQITLLDGVAIIVGVIVGAGIFVSPKGVLIHSGSVGQALIVWILSGLLSMVGALCYAELGKNLPKAICISMPVVTIIYVIANVAYFTVLTPDEMLSSEAVAVTFGDKMLGYMAWLMPFFVACSTFGSLNGAIFSSSRLFFVGARNGHLPAAISLINVHCFTPIPSLIFLGIISLVLLFIPNIYALINYVTFVEALFTLMSISGLLWLRRTQPKTERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP73</accession>
    <name>T1GP73_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02181602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GP73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005392-RA">
        <property type="protein sequence ID" value="MESCA005392-PA"/>
        <property type="gene ID" value="MESCA005392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000038_3_2_1"/>
    <dbReference type="OMA" id="CEWARNI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000223">
        <property type="entry name" value="Dynein heavy chain 3, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000044">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001328">
        <property type="entry name" value="Dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000006">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF66">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1801"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="38"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="645"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1269"/>
            <end position="1296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1826" mass="205852" checksum="18498202F1EBEFB7" modified="2013-10-16" version="1">MVNSSLPYGYEYLGNTTRLVVTPLTDRCYRTLFSALNLHLGGAPEGPAGTGKTETTKDLAKAVAKQCVVFNCSDGLDYIALGKFFKGLASCGAWSCFDEFNRIDLEVLSVVAQQILTIQRGINSGKNTLVFEGTTLKLDPTCAVFITMNPGYAGRSELPDNLKSLFRSVAMMVPDYALISEIELYSYGFLTAKPLAIKIVATYRLCSEQLSSQPHYDYGMRAVKSVLRAAGALKLRYKDENVPLFQGITSDLFPGVVLPEADYVLFNECVKLACEKNNKQVNDFFLEKVQQLYEMIVVRHGLMLVGLPFGGKTTCYRILAEALLYMNQRGHFETPAIYTIINPKAITMGQLYGQFDQVSHEWNDGILAVSYRQFAVSTTDERKWLIFDGPVDAIWIENMNTVLDDNKKLCLMSGEIIQLSPTTNLIFEPMDLEVASPATVSRCGMIYMEPSSLGWDVLVTSWKNQLPETFHTVNKQLISFMFNRFCPPLLWLVRKGGLTEIAPTSDANLVVALMNIFDCFLDDFNDPKYVESLSDLDIRSQIEGIFLFSAGLLEKVFPAELYTTFGFPEDIKVTNMQKAFIFPIPKQGTVFDYKYIKEGKGKWKPWEDEAAAAPPIPRDIPVNQIIVTTHENTRVTAIFDLLVRHSKPVMFVGPTGTGKSCYVMDYLLKKVDTEIYKPLFMNFSAQTSANQTQDIIMSKLDKRRKGVFGPPLGKKCVIFVDDISMPLKETYGAQPPIEILRMLFDHWMWYDRKNIVPLKLIDIQLHCAMGPPSTGNTVTPRFQRHFNVIAIDEFNEDLLKTIFNKIVLWHLDTRGFSKEFDPCIDEIVGATLDIYTKARMNLLPTPAKSTTFCSEAIEDMNSMRRLWVHEILRVYGDRLVDDGDRQWLFGALYSAMKNHLKSVPKVLFERIVKKGEDLVENDLRQLIYCDFTNPKADTKNYSEVLDLDELRATVEQYLVEFNNMSKKPMNLVLFTFAIEHLSRVCRIIKQPRSHALLIGVGGSGRQSLTRLASHICDYELFQVEITRTYGPNEWHEDIKLILKKISSSDLHGVFLFTDVQIKDESFLEDLNNLLNSGEVPNLFSSEEKNEICEKMRQIDKQRDKAVQTDGSQVALFNFFVQVCKDQLHIVLSMSAIGDALRIRIRKFPSIVNCCTIDWLQPWPEDALLAVSTRFLAKEDLTKLERRAAIDILKRHNYVTPTSYLELIQTFKSLLNSKRTSILQSKERYLTGIAQLEIAASQVGVMQEQLTALEPKLKISAEVVAEQVAKVQADSEIANQQRELVKLDEAAATEQAKIAQGIKDECDAKLGEAMPILNSALAALNTLTTADIAVVKTMKSPPIGVRIVMEAVCILKDVKPDRIPNPSGVGQIDDYWGASKRVLGDIKFLDSLLNFDKDNIPPRIMQKLQERILGQEAFDPEKVKSASTACEGLCKWVIALTKYDVVAKVIAPKKVALAQAEATYNAEVEWCTKKLQRAQELISGLGGEKTRWSATAKQLGETYNTLTGDVLIASGVVAYLGPFTIDFRISQTKKWVELCTHLNLVCNQEFQLATILGEPVEIRNWNINGLPTDSFSIESGIIIKNSRRWPLMIDPQGQANKWIKNMEKVNKLCVIRLNQPDYVRILENAIQFGLPVLLENIGEDIDPLLESVLLKQTFKQGGAWCIKLGDTIIEYNNAFKFYITTKLRNPHYLPEVAVKVTLLNFMITSQGLQDQVLGITVARERPDLEAEKNTLIVQGAENKRMLKETEDKILEVLSTSENILEDETAVNILSSSKVLANDINEKQAIAEVTEKQIDIARLAILLLLHILQYFSSLLVGINIYEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP87</accession>
    <name>T1GP87_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP87"/>
    <dbReference type="STRING" id="36166.T1GP87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005408-RA">
        <property type="protein sequence ID" value="MESCA005408-PA"/>
        <property type="gene ID" value="MESCA005408"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443377_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005408-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="9934" checksum="BF6FF0D16D6277CB" modified="2013-10-16" version="1">MVVATCYTLTQSCMACFNGRHACSSLCPKEQLPAPEDTDACRSPMLVEVPGHCCKLWLCENPIANAEAFEIGDDLLDITLRIYLFSGEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP99</accession>
    <name>T1GP99_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02127693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005421-RA">
        <property type="protein sequence ID" value="MESCA005421-PA"/>
        <property type="gene ID" value="MESCA005421"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017850_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005421-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="13746" checksum="3FBADF40D63B1ED7" modified="2013-10-16" version="1">MAACFGMTGCLSSTPTMSLEVFLNYRSQKYSSPTERGSFQGKKLWSQGDSFRMESIATKIVDIGKPNTDYIGQEYNFNFISNILIPERDSWKQGEILTPGELSVFTGSNGWIKDIGRYGFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPE2</accession>
    <name>T1GPE2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospholipase A2</fullName>
            <ecNumber evidence="3">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phosphatidylcholine 2-acylhydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02198093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPE2"/>
    <dbReference type="STRING" id="36166.T1GPE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005470-RA">
        <property type="protein sequence ID" value="MESCA005470-PA"/>
        <property type="gene ID" value="MESCA005470"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118255_0_0_1"/>
    <dbReference type="OMA" id="LAFYTHP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000002">
        <property type="entry name" value="Phospholipase A2 group III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12253">
        <property type="entry name" value="RH14732P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="13">
        <location>
            <begin position="7"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005470-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="16588" checksum="2B80FAF24DE8272B" modified="2013-10-16" version="1">MYSITLLSGTIWCGLGNAAPNDDALGIFRLTDNCCREHDNCPVSISANEEKFGLINTGFFARSHCDCDGRFYRCLKRARTIIAHKIGFTYFNLLRPQCFRKEHPIVTCLEGYKKSRDRCRVYLVEQSESKQWQWLTTNYSKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPF5</accession>
    <name>T1GPF5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate synthase</fullName>
            <ecNumber evidence="3">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02033610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPF5"/>
    <dbReference type="STRING" id="36166.T1GPF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005484-RA">
        <property type="protein sequence ID" value="MESCA005484-PA"/>
        <property type="gene ID" value="MESCA005484"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_3_1"/>
    <dbReference type="OMA" id="AYGRFWR"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000008">
        <property type="entry name" value="thymidylate synthase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="10">
        <location>
            <begin position="29"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005484-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="230" mass="26356" checksum="1D87B500CBAE5206" modified="2013-10-16" version="1">MAQTTFSNGESQTCPSDLKNGQSPNEDEQQYLDLISRIIAKGNDRSDRTGVGTLSIFGCQMRFNLRDQFPLLTTKRVFWRGVAEELLWFIKGQTNAKILQEKNVKIWDGNSTREFLDKCGFTDREEGDLGPVYGFQWRHFGAEYKTCHDDYSNQGVDQLNDVIQRIKNNPTDRRICMSAWNPVDLPKMALPPCHCFAQFYVQDDELSCLMYQRSADVGLGVLSILQVTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQE7</accession>
    <name>T1GQE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQE7"/>
    <dbReference type="STRING" id="36166.T1GQE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005851-RA">
        <property type="protein sequence ID" value="MESCA005851-PA"/>
        <property type="gene ID" value="MESCA005851"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533981_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005851-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9277" checksum="C505F00989E0E10A" modified="2013-10-16" version="1">MATQTSGINASLPPVSYTKAIDVWTGVCLTFVFGALLEFALVNYASRSDIHRENMKKKRRDLEQASMDAQSDLLDTDSNATFAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQF4</accession>
    <name>T1GQF4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQF4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005859-RA">
        <property type="protein sequence ID" value="MESCA005859-PA"/>
        <property type="gene ID" value="MESCA005859"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1112393_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005859-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="250" mass="27017" checksum="49E5EF41EBC668D2" modified="2013-10-16" version="1">MKQKQLNLKKLIDQIATGDAAGGSGAAAAVSGPGSVSVSVSEHNHHHQQQQHHFMMRDHVLYNGNAEFYNHFPTTDDYPEGYQRGYSSNYHSSAAAALMVVGGGNGGGTTTSSSSQDTSSFVNNCFGGDSAHHSSHHLSSHHSHILQQHHHQNHPHQLNYNNGSVPVLMQQPQQIIPSNLCQYNGGPSTGNSGLKSGLNDDDGHGATSHLKTINGDNDENEQMDEKIRDHDDDDNDNSNGGQLFVIKKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQI4</accession>
    <name>T1GQI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Receptor protein-tyrosine kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02155108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQI4"/>
    <dbReference type="STRING" id="36166.T1GQI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005892-RA">
        <property type="protein sequence ID" value="MESCA005892-PA"/>
        <property type="gene ID" value="MESCA005892"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054141_0_0_1"/>
    <dbReference type="OMA" id="ESCTRIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Furin-like cysteine-rich" evidence="4">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor L-domain" evidence="5">
        <location>
            <begin position="83"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="28345" checksum="F94D87961A768E17" modified="2013-10-16" version="1">MQRYNSVNYTWEHNPDGRYAYGATCVKACPGHLLKDSGACVRSCPINKIPKNGECVPCEGACPKRCPGVMNLVHSGNIETFRNCTVIDGSIRIIESTFKGYNEFFPNKTMSDFYPPLHPDQLEVFSNVKEITGYLDIQAFHKDFKNLSYFRNLEVIHGRILNEMHFAAFSVVQSSLESLHLKSLKRINSGTVLIQLNKNLCFVEGIDWKSIIKSSTPRIVIPPTNRKHEVCVAENKTCSGQCNYQGCWGIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR21</accession>
    <name>T1GR21_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02186408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006092-RA">
        <property type="protein sequence ID" value="MESCA006092-PA"/>
        <property type="gene ID" value="MESCA006092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1483643_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kazal-like" evidence="1">
        <location>
            <begin position="125"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="182" mass="20795" checksum="DC34F20F58D6053B" modified="2013-10-16" version="1">MINTISCWLNYFENFPISNGFAIEHFSPGALPTSDGSLFHYVNFHHPLYWNDMDFAAVFLNVKADYMNKLCPAICAKVYRPVCAFNDKLQCYKTKEECFLKSENCRRRHYNEPGGGVVQGECDPKVHKLCCPNNCPLDGPPVCGFNGNSFSKFINRCDMDVHNCKEKIMSPMYKQVDFAKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRE4</accession>
    <name>T1GRE4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein heavy chain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRE4"/>
    <dbReference type="STRING" id="36166.T1GRE4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006225-RA">
        <property type="protein sequence ID" value="MESCA006225-PA"/>
        <property type="gene ID" value="MESCA006225"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136567_0_0_1"/>
    <dbReference type="OMA" id="CTWINTD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000002">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF18">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006225-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="166" mass="19297" checksum="9D36F43458FE7FFA" modified="2013-10-16" version="1">YFGYDFECSAERGGGGGGETRESIVYQLADDMLRKLPPQYNAFEVRENLNRMGVLLPMNIFLRQEIDRMQKVIKRVQSSLSDLKLAIDGTIVMSPALKESLDAMYDARIPESWMKISWESTTLGFWYTELLERNSQFCTWINTDRPKVFWMTGFFNCQGFLTAMRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRF3</accession>
    <name>T1GRF3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006235-RA">
        <property type="protein sequence ID" value="MESCA006235-PA"/>
        <property type="gene ID" value="MESCA006235"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500480_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9400" checksum="AB4C85C4A6308761" modified="2013-10-16" version="1">MTSLSLAVKMQSSKRILRSHEIPIPQSSTSNSATVTGSSNTGTNILSSLYPNIHNSSGSGIGSGGNPSYFWLRYQQLDRHEYFHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTF0</accession>
    <name>T1GTF0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02123965">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTF0"/>
    <dbReference type="STRING" id="36166.T1GTF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006980-RA">
        <property type="protein sequence ID" value="MESCA006980-PA"/>
        <property type="gene ID" value="MESCA006980"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294845_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051583">
        <property type="entry name" value="YAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616:SF8">
        <property type="entry name" value="TRANSCRIPTIONAL COACTIVATOR YORKIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616">
        <property type="entry name" value="YES-ASSOCIATED PROTEIN YAP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006980-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11607" checksum="AF2190D875907058" modified="2013-10-16" version="1">MIQGRMECRGEAHSSPAQLQQFEPQSQKKFITNVDKSPTTSSSQQQQVAQQQVQQQQPQSMHSKQRSYDVSSIRFNNDLGELPMGWEQAKTPDGQVYYIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ4</accession>
    <name>T1GTQ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02049473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ4"/>
    <dbReference type="STRING" id="36166.T1GTQ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007093-RA">
        <property type="protein sequence ID" value="MESCA007093-PA"/>
        <property type="gene ID" value="MESCA007093"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1580297_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="32"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007093-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="18843" checksum="AE507918B22D123B" modified="2013-10-16" version="1">MEDVQYCNLRYSNHQSDLIFGIGELLFNNNLVDCTLAAEGKFLKAHKVVLSACSPYFSSFVLPSVSFCLSIILIDNEFWLLENLLGMKCLGISMTPVLSYGALVWWNTTKLGTRLNMLTSVQRLACLGIYEINTSGGVKSFVGHYVNRTAPEASKHNRRLNSKSKPGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU46</accession>
    <name>T1GU46_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104358">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU46"/>
    <dbReference type="STRING" id="36166.T1GU46"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007249-RA">
        <property type="protein sequence ID" value="MESCA007249-PA"/>
        <property type="gene ID" value="MESCA007249"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004312_1_2_1"/>
    <dbReference type="OMA" id="NWHLRFA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17301">
        <property type="entry name" value="PIPKc_PIP5KI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF101">
        <property type="entry name" value="LP03320P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="198"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="725"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="778"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="681"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="695"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="785"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007249-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="809" mass="89462" checksum="6979BBC86CB9BE8A" modified="2013-10-16" version="1">MTVLKLNLVFLAREQILYFFLNEEKFVFEIRSDDIPIQDDDKHLTSISEEPDQQQVGPRNTAIRSSKEGIKAQAHKALAGFKKSKDKEKDTSKNTPNKKDEAPPKPPSRKISTTSLVGSGPNVAKSNLDDDPPKPGPGRTASALKNALNQAANIGVASLNAAAAGAGSDDGAKLADKERKIGHRRVGEGGEITYKKIQTSTIMGSIQLGIQHTVGSLASKPKRDLLMMDFCEIESISFPPEGSTLTPAHHFSEFRFKIYAPIAFRYFRDLFGIEPDDFMMSMCSAPLRELSNPGASGSIFYLTDDDEFIIKTVQHKEVAMNNLLPSDVKMHLKYDLKGSTYKRKASKAERQKSSPTFKDLDFMEHHPNGIFLEADTYQALIRTMQRDCRVLESFKIMDYSLLLGVHNIDMANKEKQEANKNTAGTSGLDNRNRVPDTSYGSELESDLEDAPEADQCIAQDKAGNTALNRSRSINRQRLVAHSTAMESITDAKFEPIEEEGDEDTPTGGIPARNEKGERLLLYIGIIDILQSYRLKKKLEHTFKSIFHDGDTVSVCRPSFYAQRFQNFMEKTVFRKVPSTLKQQPSKKRNILRMAQKPSNPDSESSMPSGKFQTNAVPSCSSTPPPAFDDIDEKHNNPMKEETQQMVAPATPEPRISTPRKKDSVPPPPPPRSIHQQQTVMSSTTTTTKYISKSSNQASPSSGRIQQQQYDAVSISEIRLDTHLAVDTSSSNYGSRGALAWTPPASYEGSTPTWTEGTPSYTDSSDSCLEDLDLGAVANSPNHQIQYRSGSSQSYNRSNSSHSSGTGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU50</accession>
    <name>T1GU50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007257-RA">
        <property type="protein sequence ID" value="MESCA007257-PA"/>
        <property type="gene ID" value="MESCA007257"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657297_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007257-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8888" checksum="EE0A9BD5DD18E69C" modified="2013-10-16" version="1">MRIPTFINSFSGHEYIAFETAFSQTCKPPFRNPRKADWKKFKTIATARINQQQRDQPYGYSLLDTGNLNRMGIPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU77</accession>
    <name>T1GU77_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02172696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU77"/>
    <dbReference type="STRING" id="36166.T1GU77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007285-RA">
        <property type="protein sequence ID" value="MESCA007285-PA"/>
        <property type="gene ID" value="MESCA007285"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2190544_0_0_1"/>
    <dbReference type="OMA" id="ACPYIND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="27"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12493" checksum="A55F9E887A191547" modified="2013-10-16" version="1">MKKKSKSKKRGLQLRERDESKELVQAPHSFVIARGFACPYINDLVKDFRKVLEPFTAANLKEKKNNKIKDYISLAGVFHVSHLCIFNKASNQLSFKVVKTPRGPTLTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUH1</accession>
    <name>T1GUH1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC-2 type transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUH1"/>
    <dbReference type="STRING" id="36166.T1GUH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007384-RA">
        <property type="protein sequence ID" value="MESCA007384-PA"/>
        <property type="gene ID" value="MESCA007384"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_761389_0_0_1"/>
    <dbReference type="OMA" id="WICTRTA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="33"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007384-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="364" mass="41414" checksum="9533155B50EFE313" modified="2013-10-16" version="1">MTLMERSNISDIPELTSIANEYGKYIEDETSTANFKEINEGFEDYILNLYKSILVRVNTKYVAAATFYAQNITAWLNNQPFHAAPLTVNLIHNAMARAYLGHEYRISVTNSPLPYRPDSKFFQLLIGNSLGSQLSSNTCFCMCFVSAMYILFLIKERVSRSKLLQFVGGVKVWTFWLTQIIWDYFTYLVTALVFVGTIACFQEENFSTFSELGKYYFLLMIFGISVLPFTYLMANLFTEPAIGFGRISILNLFAGNALFIVIMVMKMPFLDTAPVANALQWVFNIFPHYALASSMSKLSNNIATRKVCDEVCSLIPILKDCTAEVLCEKMEGKNVVCNLSLDGPNLEFYLKFFTCWPVSLFYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUN0</accession>
    <name>T1GUN0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUN0"/>
    <dbReference type="STRING" id="36166.T1GUN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007447-RA">
        <property type="protein sequence ID" value="MESCA007447-PA"/>
        <property type="gene ID" value="MESCA007447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032401_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13465" checksum="E5C103DFA3281E38" modified="2013-10-16" version="1">MSNSLVNPLIYGAFHLCPLNRKDSKYGQYSLTRDNSQRSPSLMTAVTQIDCNGRQIRTFRQPSYYRTNSNNSQKEQITLLHSGLTEKHPSSTSPSPINRQHMSRHKSSNGLLGGTGPGTGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV42</accession>
    <name>T1GV42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">S-adenosylmethionine mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV42"/>
    <dbReference type="STRING" id="36166.T1GV42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007624-RA">
        <property type="protein sequence ID" value="MESCA007624-PA"/>
        <property type="gene ID" value="MESCA007624"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_0_1"/>
    <dbReference type="OMA" id="IGPRTMW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000018">
        <property type="entry name" value="S-adenosylmethionine mitochondrial carrier protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="87"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="182"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007624-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="30868" checksum="25636AFCC201E459" modified="2013-10-16" version="1">MSGENHYFRSLVSGGVAGLVVDIVLFPIDTVKTRLQSEAGFFKSGGFRGVYKGLAPAAAGSAPTAALFFCTYESCKSYLSTVSGNKNLPVVHMVSASIAEVVACLIRVPVEIAKQRRQALIVTGNQSAFKILYNAVKTEGIRKGMYRGFGSTIMREIPFSFIQFPLWEYFKLNWSEVTGTPLTPVSVALCGAVAGGISAGITTPLDVAKTRIMLSQQVGGRRMGVFNILSNIYREKGIQGIFAGFTPRVLWITLGGAIFFGFYDLTSRIIDSSRHENSVDGGSISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVA0</accession>
    <name>T1GVA0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007690-RA">
        <property type="protein sequence ID" value="MESCA007690-PA"/>
        <property type="gene ID" value="MESCA007690"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815391_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007690-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7513" checksum="0BE71F6A55B3CEBC" modified="2013-10-16" version="1">MMLMIEKIAYQFSSYRVLEPGKKPKKSRILQVAISRLSESASSCQFASSSKRIFGDSLFKAVVLNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVQ4</accession>
    <name>T1GVQ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVQ4"/>
    <dbReference type="STRING" id="36166.T1GVQ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007870-RA">
        <property type="protein sequence ID" value="MESCA007870-PA"/>
        <property type="gene ID" value="MESCA007870"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_4_1"/>
    <dbReference type="OMA" id="IRYNITR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000036">
        <property type="entry name" value="Trypsin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007870-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="200" mass="21630" checksum="338DA5423EC7F110" modified="2013-10-16" version="1">SIISNEHVLTAAHCLKGQKKSRLSILAGVTNLNQKSGSRHNVKSFAIHRDYKELVRNDIALMKVSPPFQFDGKAIGPIRYNITRKIGGGESVMLTGWGSISPSTLGPLPKDLQYLKLKTITNNECNRLVLNVSKTEVCAFRSPFSGACAGDSGGPLALEDQSLLVGVVSYGTLICAVGLPDVYTRVSVLSGWIEETMRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVX9</accession>
    <name>T1GVX9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02124930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVX9"/>
    <dbReference type="STRING" id="36166.T1GVX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007947-RA">
        <property type="protein sequence ID" value="MESCA007947-PA"/>
        <property type="gene ID" value="MESCA007947"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016631_3_1_1"/>
    <dbReference type="OMA" id="EEPNCTM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000013">
        <property type="entry name" value="E3 ubiquitin-protein ligase MGRN1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045194">
        <property type="entry name" value="MGRN1/RNF157-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058981">
        <property type="entry name" value="MGRN1/RNF157-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996">
        <property type="entry name" value="MAHOGUNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996:SF0">
        <property type="entry name" value="RE60872P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26192">
        <property type="entry name" value="RNF157-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007947-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="316" mass="35340" checksum="C6FCEE4EB8F28AB9" modified="2013-10-16" version="1">FPYPPPQANEPTKTLKSLVNIRKESVRFVKVPNDKPAEVEGASPQNTYNIDFTFDADAKVAITIYYFCSEEISPSGVTLVPKDSLTSGTFHYKKGYNQQFSQPTHIFNPNLFAEDDLSFNQDNTHFPVAIHCVVEESNEDCKHSHTTICVIDRHTDGTYTLRALKQKIFVDGLCYLLQEIYGIENKALNKVAIDDETDDNGSECVICMSDIRDTLILPCRHLCLCNSCADSLRYQANNCPICRAPFRALLQIKAVQKKMGSSNNYNTQSQPNNENIPNGYEVVSLIEALNGPPQYLNTSVISSPIEIDKLEDGGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW63</accession>
    <name>T1GW63_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02091077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02091078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW63"/>
    <dbReference type="STRING" id="36166.T1GW63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008035-RA">
        <property type="protein sequence ID" value="MESCA008035-PA"/>
        <property type="gene ID" value="MESCA008035"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="OMA" id="TSGSWWC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588560" description="Peptidase S1 domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="35"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008035-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="26203" checksum="97BFBE38C94E3D46" modified="2013-10-16" version="1">MKLFFLLSTLLVAASATNFRFPASKLKDILPDSRIVGGQLANAGQFPYQVGLSLSDAWVYAGTLNVNTLDSTAKAIQVTSASSFKIHANYNPNTLNNDISVIRLPSSLPMTYYIWPVVLVSRAEAGNTFEGQTAIASGWGKIADSTSSITNLLRWTDLNVEKQNTCKSYYMAGLVTDGVICTNTKGGAVSTCNGDSGGPLVLKSTGRQIGVTSFVSSAGCQSGGPDGFTRVTYYLDWIKQNTGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWA5</accession>
    <name>T1GWA5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02079173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWA5"/>
    <dbReference type="STRING" id="36166.T1GWA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008078-RA">
        <property type="protein sequence ID" value="MESCA008078-PA"/>
        <property type="gene ID" value="MESCA008078"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058290_0_1_1"/>
    <dbReference type="OMA" id="ERETHIY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="2">
        <location>
            <begin position="4"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008078-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="20378" checksum="F9394885D57307ED" modified="2013-10-16" version="1">MSERENNVYKAKLAEQAERYDEMVESMKKVASMDLELTVEERNLLSVAYKNVIGARRASWRIITSIEQKEENKGAEDKLEMIKNYRGQVEKELRDICSDILDVLEKHLIPSAVSGESKVFYYKMKGDYHRYLAEFATGTDRKEAAENSLFAYKTASDVAMTDLPPTHPIRLGLALNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWH9</accession>
    <name>T1GWH9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008156-RA">
        <property type="protein sequence ID" value="MESCA008156-PA"/>
        <property type="gene ID" value="MESCA008156"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_977581_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008156-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="285" mass="31490" checksum="0269219916264034" modified="2013-10-16" version="1">MTVSFPGNSNSTAHLVENEHVPLSHLDVIHSPHSQTNTPNGHEITQTHDSTLDEVALQQQQQLHQRPQKHSETDDDEDDDGDLTDNMDDGAGIEFKQFRSNQIRRHRWSFNLEQLENPTINYNLMKSASGSENVYSNILPVTAAQVQTSVVATAAAPKESETVDIETDPNLHLYSNINQENFVKSSKSTAQNNPLSTNNNIQSAYLESSDIMSSTLYNDDLDLDDPVQAFVNKSSLIPQSKSNISDGPEAHRLRLLHQSTMINTALDLDSIEDTSLGTSSQAAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX22</accession>
    <name>T1GX22_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the C-terminal section; belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX22"/>
    <dbReference type="STRING" id="36166.T1GX22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008360-RA">
        <property type="protein sequence ID" value="MESCA008360-PA"/>
        <property type="gene ID" value="MESCA008360"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_710363_0_0_1"/>
    <dbReference type="OMA" id="NENICAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000007">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="19" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 22" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="15 22" id="KW-0720">Serine protease</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="23">
        <location>
            <begin position="162"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008360-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="389" mass="42505" checksum="170353892762A616" modified="2013-10-16" version="1">MKPGKPMTFASNYDNGKFFFGLPGNPVSAFVTFHLFVLPALRYTSGWKKDKCSLAIVEVELLNDVIQLDSRPEYVRASVTSKNGKLFAKVNGNQMSSRLQSIVGADVLIHLPGSTESRPSALKGDIFKASVLRYDFISSYDFTCALGSSWSIDPTPADDGRIVGGFNIDITKVPYQISIQTPWESHFCGGSLISKNMVLTAAHCVDDNPDPKDVIIYMGATNRTAGGIRIRAENIVHHENYTEKPVMNDIALIKLSRSIKLSNKVKIIKLLTREPAPRSKALATGWGKLRQTDEDYQIPELLQGVNLDTISLATCTKVYGSNRIQNENICAYTDGKDTCQGDSGGPLAYRGKLLGVVSWGAGCAQKGSPGVYASVPKFLPWIRKTIASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX80</accession>
    <name>T1GX80_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02191087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX80"/>
    <dbReference type="STRING" id="36166.T1GX80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008422-RA">
        <property type="protein sequence ID" value="MESCA008422-PA"/>
        <property type="gene ID" value="MESCA008422"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_8_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF37">
        <property type="entry name" value="GH01208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008422-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10518" checksum="97FC502F9A134BB3" modified="2013-10-16" version="1">MILTISALDPAFPPFYMGKLTKHISKKDAEFVDIIHTDAWIYGAPFSTGHADFWPNSGKTLQPGCPRRNYKMLTDNDLSSHRRSWWFWAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXG3</accession>
    <name>T1GXG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4766 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02042441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008514-RA">
        <property type="protein sequence ID" value="MESCA008514-PA"/>
        <property type="gene ID" value="MESCA008514"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2139704_0_0_1"/>
    <dbReference type="OMA" id="ATACCAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577805" description="DUF4766 domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008514-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="10102" checksum="7EBD3DC5F8933F04" modified="2013-10-16" version="1">MAKIFVFFLIYLVVKCRSQGLGGGFGGGVQVGIGANSGGGGAGLGGGIGFGANAGIGAGGGYGGPPGGGGFSGGPPSDGQGGGQADFSGGYGGPGGDQSASNSPPNSAYGSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXS3</accession>
    <name>T1GXS3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXS3"/>
    <dbReference type="STRING" id="36166.T1GXS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008628-RA">
        <property type="protein sequence ID" value="MESCA008628-PA"/>
        <property type="gene ID" value="MESCA008628"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021022_0_0_1"/>
    <dbReference type="OMA" id="PQISAGE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008628-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="11625" checksum="A52CC6BA31E0F6A4" modified="2013-10-16" version="1">GSGGQDFGANGYSSGRPGGSGGQDFGANGYSSGRPGGSGGQDFGANGYSSGRPNGSGGLNNGRSGGNNRSGSQGQDNRNGSGYSSGRPQNGSGGGGRNGNRGNGSGFGRGGGNGQNDGSGYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXU7</accession>
    <name>T1GXU7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXU7"/>
    <dbReference type="STRING" id="36166.T1GXU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008656-RA">
        <property type="protein sequence ID" value="MESCA008656-PA"/>
        <property type="gene ID" value="MESCA008656"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2226194_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="11925" checksum="7A089BBB548C4F97" modified="2013-10-16" version="1">MNIMPPSSMPEVDTAGSTEDWSGFEASYSKSTGAYKYSDLENSFRLIEALKGPAKEAVDGLLIHPENVNEVMKKLRFEFRRSDLIVKCQHEKIMKLPAIDESDLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY00</accession>
    <name>T1GY00_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sugar phosphate phosphatase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
            <ecNumber evidence="10">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism. Has also been shown to have O-methyltransferase activity that methylates glutamate residues of target proteins to form gamma-glutamyl methyl ester residues. Possibly methylates PCNA, suggesting it is involved in the DNA damage response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>beta-D-fructose 6-phosphate = dihydroxyacetone + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:28002"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="domain">
        <text evidence="10">Subfamily III proteins have a conserved RTxK motif about 40-50 residues from the C-terminus; the threonine may be replaced by serine or cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EC" id="3.1.3.-" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02372794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY00"/>
    <dbReference type="STRING" id="36166.T1GY00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008715-RA">
        <property type="protein sequence ID" value="MESCA008715-PA"/>
        <property type="gene ID" value="MESCA008715"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1566807_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097023">
        <property type="term" value="F:fructose 6-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103026">
        <property type="term" value="F:fructose-1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039763">
        <property type="entry name" value="ARMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260:SF6">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="11">
        <location>
            <begin position="46"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008715-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="171" mass="20384" checksum="81EC49E23FC0A4C5" modified="2013-10-16" version="1">MSSEAGKDDFQLSKPEFPDRYNLIDSKTPPLTKLSGKFKRSFAYHTLNERIPVILTQLIDNLYKGVDDIARDISEDEEVKKYQKTLELFDENREKDDLDVFVWNNFIRKLPEDANTFYFTCWLYAECYVYRRIKQFFENFKVLGNFDYFHKQKFDALQSSLKPLSDILVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY56</accession>
    <name>T1GY56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitinyl hydrolase variant UBP zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02111650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY56"/>
    <dbReference type="STRING" id="36166.T1GY56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008777-RA">
        <property type="protein sequence ID" value="MESCA008777-PA"/>
        <property type="gene ID" value="MESCA008777"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909070_0_0_1"/>
    <dbReference type="OMA" id="TIDCQPE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitinyl hydrolase variant UBP zinc finger" evidence="1">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008777-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15173" checksum="C05C37EEB46DC2C7" modified="2013-10-16" version="1">MNYYQISSFNVYKDECLYSFDNPESSTGIYISLSSFLCFGEEFVDLYSKASGTHIFLHLHRTKKLVNKTELSTIDCQPEPKITKLAIGIEGGFQPNSNKFEYQDNYNIVVFPGKVIVPYPNEDLPLKGQIRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYI9</accession>
    <name>T1GYI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375598">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYI9"/>
    <dbReference type="STRING" id="36166.T1GYI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008918-RA">
        <property type="protein sequence ID" value="MESCA008918-PA"/>
        <property type="gene ID" value="MESCA008918"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329226_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039802">
        <property type="entry name" value="MTMR14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13524">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13524:SF2">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008918-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23179" checksum="47BE7F8BA8B1A39E" modified="2013-10-16" version="1">MTYLTLAYDWYLFGHQLPDRLKRGEDIMFFCFHVLKYITEDEFSIVEHRKRTKTTSSSGSSSVIILKSNGENGGDDDSIKDSDELDYIFNVDQDSNDSFSNVSNCDMSITENFYTSNITSRSPNPKRSKTSPISVPRQRHESTSSNGSWQVVTDTGSIDSIMNGSYMTRFIHQEHNGTNGANGTNGYQSGSSCNSNITAKDLRNAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYW2</accession>
    <name>T1GYW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02154302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYW2"/>
    <dbReference type="STRING" id="36166.T1GYW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009049-RA">
        <property type="protein sequence ID" value="MESCA009049-PA"/>
        <property type="gene ID" value="MESCA009049"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2405691_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="5">
        <location>
            <begin position="36"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009049-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10094" checksum="462ECD95B1AAE10D" modified="2013-10-16" version="1">MLYSEIKSTILGAKLSDQEANGAATFIPPMNFNYSDEMDWRQHGAVTAVKNQGQCGSCWSFAATGALEGQHFRKTGKLVSLSEQNLVDCTGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ42</accession>
    <name>T1GZ42_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009134-RA">
        <property type="protein sequence ID" value="MESCA009134-PA"/>
        <property type="gene ID" value="MESCA009134"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582576_0_0_1"/>
    <dbReference type="OMA" id="GACLESD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF12">
        <property type="entry name" value="OSIRIS 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588623" evidence="1">
        <location>
            <begin position="28"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009134-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="18855" checksum="25BEB9F0C61F58AF" modified="2013-10-16" version="1">MSIFKKIKFLLVFVLCGSVGQIQVLCASPVDSEFSPASRAMIKVYDECSQAEFGFVSCLKKKSITFIDRISNLDAITVSEGVRIIRLPDAKPTGKSLSENDLESSLPRTIEDRDGKLTTMLFDRLSRYFNGHTIMISFPKMSSEEIGRGLEEGRGKMKKMMGMMMMGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZH1</accession>
    <name>T1GZH1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009272-RA">
        <property type="protein sequence ID" value="MESCA009272-PA"/>
        <property type="gene ID" value="MESCA009272"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3020306_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577766" evidence="1">
        <location>
            <begin position="17"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009272-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="56" mass="5756" checksum="44EEA1202465F4D5" modified="2013-10-16" version="1">MFKLFVLSCLIAIAAAAPAPGVVVAAPIVHHAPILHPAPILHHAPIIHHAPVAVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZP8</accession>
    <name>T1GZP8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZP8"/>
    <dbReference type="STRING" id="36166.T1GZP8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009352-RA">
        <property type="protein sequence ID" value="MESCA009352-PA"/>
        <property type="gene ID" value="MESCA009352"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029359_0_0_1"/>
    <dbReference type="OMA" id="REDWSIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF60">
        <property type="entry name" value="STRIPE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13990" checksum="91C87C8E37D84C3B" modified="2013-10-16" version="1">MRIHTGEKPYICEVCNKAFARRDKLVIHMNKFKHITPTNIAPLGKRLNNIIKKKEPDIEESKIINIQQTVPQNITVHPSSSSVTTGILVSQQQHTTWSCELCGRLFASREDWSIHAKSHLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZS7</accession>
    <name>T1GZS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Medium-chain acyl-CoA ligase ACSF2, mitochondrial</fullName>
            <ecNumber evidence="4">6.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a medium-chain fatty acid + ATP + CoA = a medium-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48340"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:59558"/>
            <dbReference type="ChEBI" id="CHEBI:90546"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>octanoate + ATP + CoA = octanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33631"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02178855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZS7"/>
    <dbReference type="STRING" id="36166.T1GZS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009383-RA">
        <property type="protein sequence ID" value="MESCA009383-PA"/>
        <property type="gene ID" value="MESCA009383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617206_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.38.10">
        <property type="entry name" value="Luciferase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="10">
        <location>
            <begin position="54"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="183"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="444" mass="49114" checksum="667E7803C18B55F1" modified="2013-10-16" version="1">MFTGYYTDDSDSEMTSTLASLSADASTSAAAAGLMMISGDVSPTNCGSRGPYERAWTTDATRALVHIRGSMEEKFNDSRCKRTKLWSQVAKQLYKLGYKYNSAKVQKKWHNILITYNKNLNKKYATGYVHWEFFEEMYKYLQGKRASLIGTSTVTSSSSLHQAATTTSSNNNSGNLNNSPDDMLLRDDLPYEPERRIKTEYSINDSENNDLENSPCNIQFTSGTTGAPKAAVLSHYNFVNNSIHVGNRNEYRNERICCQVPLFHAYGVVISIMGGISHAATLVLPSAGYNPEASLQAIANEKCTVIHGTPTMYVDLIKKQKELNLEIGSAKYAITGGAACSPQLFEDIKNVLGLKKVKTVFGMTETSAVIFQSMQEETKDNVLHTVGHVQDHVEVKVIDANGNTVPFGTPGELCIRGYCNMLGYYGDEEKTKEIMSSDRWLKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0G7</accession>
    <name>T1H0G7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyl-D-glucosamine kinase</fullName>
            <ecNumber evidence="2">2.7.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GlcNAc kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type N-acetylglucosamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.59" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02388267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0G7"/>
    <dbReference type="STRING" id="36166.T1H0G7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009645-RA">
        <property type="protein sequence ID" value="MESCA009645-PA"/>
        <property type="gene ID" value="MESCA009645"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108972_0_0_1"/>
    <dbReference type="OMA" id="CKSIFRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045127">
        <property type="term" value="F:N-acetylglucosamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039758">
        <property type="entry name" value="NAGK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862">
        <property type="entry name" value="BADF TYPE ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862:SF0">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009645-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="19044" checksum="047A1D110B82C263" modified="2013-10-16" version="1">GTGSNALLKNPDGSFYGCGGWGNFIGDEGSAWYMAFRAIKLCYDDMDNFRPSIYSTERVWELIQQHFNITTRLEILDHCYAKFDKPFFAGLCAKLAEAAKEGDELCKSIFRETGEYLAKHIIALLPKVQDELVANGDLSIVCVGSVWLSFDLFQESFLKNYPNTNLNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Q1</accession>
    <name>T1H0Q1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Q1"/>
    <dbReference type="STRING" id="36166.T1H0Q1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009736-RA">
        <property type="protein sequence ID" value="MESCA009736-PA"/>
        <property type="gene ID" value="MESCA009736"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592522_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009736-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8864" checksum="E196219496C88CF9" modified="2013-10-16" version="1">MNSKFTKSGRNMPEWVSNTNGRIGPKPPPPPSGSPSDPKPSLPPKRNDKEPDYEVIEFGQYSNVQKPKTPGTFLDLLQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H194</accession>
    <name>T1H194_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H194"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009948-RA">
        <property type="protein sequence ID" value="MESCA009948-PA"/>
        <property type="gene ID" value="MESCA009948"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3426993_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009948-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="21" mass="2527" checksum="3470E832C8D64A55" modified="2013-10-16" version="1">MDSRVNPTTNFYNFEKHLLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H223</accession>
    <name>T1H223_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H223"/>
    <dbReference type="STRING" id="36166.T1H223"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010254-RA">
        <property type="protein sequence ID" value="MESCA010254-PA"/>
        <property type="gene ID" value="MESCA010254"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344637_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010254-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23702" checksum="EA265DF0BABF5EBE" modified="2013-10-16" version="1">MGRKMSEEGETDLLPFYQKVLSFGTIVILQPWFNIDFIIKRWKHYPEIFNAINMISNFSKSLIQGKIDEINSNKIADLEENSKKKSIFINQTLSMVQKNEFNWREVEAESNVMIFGAFETTASVLYSVLMCLSFYPEYQEKVYEEIMSVIPEDGDINYEHIKDLSYMEMVINETLRILPAVPLVGRRVAEDMNLGKLHVPKGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H233</accession>
    <name>T1H233_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02383504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H233"/>
    <dbReference type="STRING" id="36166.T1H233"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010264-RA">
        <property type="protein sequence ID" value="MESCA010264-PA"/>
        <property type="gene ID" value="MESCA010264"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742931_0_0_1"/>
    <dbReference type="OMA" id="KLLAMIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF431">
        <property type="entry name" value="TETRASPANIN 26A, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7837" checksum="6F3BEA9798FE01AB" modified="2013-10-16" version="1">MTGLVVLAIGIWAWTEKDMFSNISKLTFIALDPAFVLIVIGTITFIIGFTGSVGALRENTCMLYMYAIFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H286</accession>
    <name>T1H286_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H286"/>
    <dbReference type="STRING" id="36166.T1H286"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010323-RA">
        <property type="protein sequence ID" value="MESCA010323-PA"/>
        <property type="gene ID" value="MESCA010323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010358_0_0_1"/>
    <dbReference type="OMA" id="AHEAPND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000037">
        <property type="entry name" value="E3 ubiquitin-protein ligase nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="110"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="276" mass="32078" checksum="38E2273551EC90C7" modified="2013-10-16" version="1">LSGVQTPTSNNGLPPNWEERQDALGRTYYINHASRTTQWARPTLADTNNRVEAMTNDFQRRFHISADESEENRNQNEDQTDGENRSETSSPVAHEAPNDDGSQLSNEEEESLPPGWSMQVAPNGRTFFIDHTARKTTWIDPRTGRASPMPNQTRRVEDDLGPLPEGWEERVHTDRRVFYIDHNTRTTQWEDPRLSNPSIAGQAVPYSRDYKQKYEYFKSQLKKPNNVPNKFEIRVRRSSILEDSYRIINSTNKMDLLKTKLWIEFEGETGLEFIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2H8</accession>
    <name>T1H2H8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2H8"/>
    <dbReference type="STRING" id="36166.T1H2H8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010424-RA">
        <property type="protein sequence ID" value="MESCA010424-PA"/>
        <property type="gene ID" value="MESCA010424"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123879_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12810" checksum="2B7FEF0D4878FA30" modified="2013-10-16" version="1">MKHTIVLSRYHHVNGISVVETDHYRAVSILKACGAVLVLVVSREVTRLIGHPVFSDDGSVSQISVETRPTKYIPPPIQILPEHQTLVHPEETFQKEYIQAPEQQGVIENGKDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1H378</accession>
    <name>T1H378_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H378"/>
    <dbReference type="STRING" id="36166.T1H378"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010697-RA">
        <property type="protein sequence ID" value="MESCA010697-PA"/>
        <property type="gene ID" value="MESCA010697"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_862145_0_0_1"/>
    <dbReference type="OMA" id="ESHYFAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF116">
        <property type="entry name" value="FI16631P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010697-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="323" mass="35638" checksum="25A7B417BFA40A5A" modified="2013-10-16" version="1">MLRRSSYSSATQILKVDKAIVNTEYNRNTMKNDLALLHIEAPLKFNRWVKPICMPQKGRTNQNNDWKWGPTPGTICIVAGWGALREKGPASDPLKEVQVPIVSNCTERDDIEAGDICAGDVSGGRDACQGDSGGPLFCKSVSNEQEWYLAGVVSHGNGCARPGEYGVYTRVAIYLEWIKNSINYFESEAGATNLQCPGYVCIWGGKRCIPGSRRCDRVVDCLGGEDEIGCIYNYIPDVGSVLTTTSSYEEQDGIVENTSETIITSTPTLFSVNTFQNNLDENDVRIDISKSTESISSTEISEIMNNFFSESHYFAKQKLSNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3X8</accession>
    <name>T1H3X8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3X8"/>
    <dbReference type="STRING" id="36166.T1H3X8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010975-RA">
        <property type="protein sequence ID" value="MESCA010975-PA"/>
        <property type="gene ID" value="MESCA010975"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987152_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031213">
        <property type="term" value="C:RSF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028938">
        <property type="entry name" value="Rsf1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296:SF3">
        <property type="entry name" value="DIKAR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296">
        <property type="entry name" value="REMODELING AND SPACING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010975-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="15040" checksum="8B8E5ACAB4E99291" modified="2013-10-16" version="1">DLEQLLLDTTDAERNSNSLPELIIRFLRGFSIYKNYKNQITTSNYQMYLRRLFRKKCDKLYLENHFDSDVDFTSLPVRNKLLILYTLCHLRLEPENHNVSEVFNNLEADSIRVQPLGFDSKKSSYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H496</accession>
    <name>T1H496_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small nuclear ribonucleoprotein Prp3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024131">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H496"/>
    <dbReference type="STRING" id="36166.T1H496"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011102-RA">
        <property type="protein sequence ID" value="MESCA011102-PA"/>
        <property type="gene ID" value="MESCA011102"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256784_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd24163">
        <property type="entry name" value="RWDD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017359">
        <property type="entry name" value="Phi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059181">
        <property type="entry name" value="RWDD2A-B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955">
        <property type="entry name" value="RWD DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955:SF8">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small nuclear ribonucleoprotein Prp3 C-terminal" evidence="1">
        <location>
            <begin position="34"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011102-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12337" checksum="29C8561FC27E2237" modified="2013-10-16" version="1">MSWLQDNLEDYIKQDQCSEITSKDEELVDFERLWIYSHHIKSKTKRKNIIQNANELDLSGFMRPGKPGVICVEGLKSNTTEFYKIIKSWTWQKITIRSNEVKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H519</accession>
    <name>T1H519_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H519"/>
    <dbReference type="STRING" id="36166.T1H519"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011392-RA">
        <property type="protein sequence ID" value="MESCA011392-PA"/>
        <property type="gene ID" value="MESCA011392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108644_0_0_1"/>
    <dbReference type="OMA" id="MATYWAT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF494">
        <property type="entry name" value="GH04538P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="7"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18510" checksum="509439DC5262AFA8" modified="2013-10-16" version="1">MATYWATACVYVLFISTSFHDVINYDLEIDWDKRIYIAIVSVPIILIGQIRNLKFLIPFSASANFLIFMTFGITLYYMFRDPLVYSDKPLYAGYKTLPLFFSTVIFAMEGIGVVMPVENEMRTPKHFLGCPSVLNTVMFIVITFLTIIGFFGYADSTIVTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H544</accession>
    <name>T1H544_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02174458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H544"/>
    <dbReference type="STRING" id="36166.T1H544"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011419-RA">
        <property type="protein sequence ID" value="MESCA011419-PA"/>
        <property type="gene ID" value="MESCA011419"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1696983_0_0_1"/>
    <dbReference type="OMA" id="HIPITIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004307">
        <property type="term" value="F:ethanolaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF71">
        <property type="entry name" value="FI05338P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011419-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="17540" checksum="38BC782BBE96B9B2" modified="2013-10-16" version="1">MISYKYKTGKMRNYSEAMRPMYPFLIFMFLLILWPIISPNSICDKDPRVLFLLSGTIFSNISCRLIVSQMSNTRCEVYHWMMPVYIIFIILSLWIPQLERILLYTLCLITTLSHWHYGSRVVQVMCVHFNRACFSTNPRNAIDEPKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5S2</accession>
    <name>T1H5S2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5S2"/>
    <dbReference type="STRING" id="36166.T1H5S2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012055-RA">
        <property type="protein sequence ID" value="MESCA012055-PA"/>
        <property type="gene ID" value="MESCA012055"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_976496_0_0_1"/>
    <dbReference type="OMA" id="ANMMVRV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012055-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="312" mass="36496" checksum="BE4454FD79C53F8F" modified="2013-10-16" version="1">DMKTMEILVSIMSKVFPDFKIKWLVDETKLNLPIELDFLHEGKNCEKIAEMFKKEYKWLKVPKIYWEHSTSRVLVMEYLEGGHVTDVDYLKDNKIDPFEVSNKIGQLYSDMIFVKGFVHSDPHPGNILVKKNPETGKADIILLDHGLYANLTNKFRYEYSKLWLSILNVDRNAMKKHSMNLGIKGDLYGLFACMVTGRPWELVIQGIDKELLQNNSQMVIPQLSDVLEQVDRQMLLILKTNDLIRGIESTLGTKNRMTAFWVMSKCCVKSSYKEEISTEDRRIARWRLIFSEKWAIFKLNIYYLYLGLINYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H611</accession>
    <name>T1H611_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FOXP coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H611"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012144-RA">
        <property type="protein sequence ID" value="MESCA012144-PA"/>
        <property type="gene ID" value="MESCA012144"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036546_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050998">
        <property type="entry name" value="FOXP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032354">
        <property type="entry name" value="FOXP-CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45796">
        <property type="entry name" value="FORKHEAD BOX P, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45796:SF4">
        <property type="entry name" value="FORKHEAD BOX P, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16159">
        <property type="entry name" value="FOXP-CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FOXP coiled-coil" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="269" mass="31082" checksum="BD500C9C68E22D89" modified="2013-10-16" version="1">EHVLDEKSTAQARVQMQVVSQLELHLQKERDRLQAMMHHLFLKQSPKKREFKATEAHIRTPICPVGIPSRMLIAISLRSPIVNNANYRKRISDRNSLSLSGGLPYMLERAGLDVQQEIQRNREFYKNADVRPPFTYASLIRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNAIRTNLSLHKCFVRYEDDFGSFWMVDDNEFVKRRHLSRGRPRKYEPSSPTHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6H4</accession>
    <name>T1H6H4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6H4"/>
    <dbReference type="STRING" id="36166.T1H6H4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012307-RA">
        <property type="protein sequence ID" value="MESCA012307-PA"/>
        <property type="gene ID" value="MESCA012307"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053914_0_0_1"/>
    <dbReference type="OMA" id="FLTEITW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008116">
        <property type="entry name" value="SSDP_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610:SF12">
        <property type="entry name" value="SEQUENCE-SPECIFIC SINGLE-STRANDED DNA-BINDING PROTEIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610">
        <property type="entry name" value="SINGLE STRANDED DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04503">
        <property type="entry name" value="SSDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01743">
        <property type="entry name" value="SSDNABINDING"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012307-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="274" mass="28921" checksum="F13BC25717327E5F" modified="2013-10-16" version="1">YAKTKNSSVPSDAQARDKLAVYVYEYLLHVGAQKAAQTFLTEITWEKNITLGEPPGFLHTWWCVFWDLYCAAPERRDSCEHSSEAKAFHDYSAGLRPPPPSQMMPGQPFMGGVPRYPGPGPSGPRPNVRMPQGMGNDFSGPPGQPMMPNSMDPTRQGAPPGMVGPMNHRMNAPHGPNMSGGPMGPGSYGGAGMRGPTPGGPGVPPGMQMGGCGRPQMPPNTSSPMNYPLTSPGNYGGPSGPNGQPGTPLTPIMPSPQDSSNSGGNNIYQSMMKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6M6</accession>
    <name>T1H6M6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6M6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012359-RA">
        <property type="protein sequence ID" value="MESCA012359-PA"/>
        <property type="gene ID" value="MESCA012359"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_680750_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="7">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012359-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="405" mass="44667" checksum="A2F2DA7648210955" modified="2013-10-16" version="1">DIEKAFFLLLLVVLIGEFFSAPAVTLADSAVITLLGDDADKYGQQRMFGSLGWGISMFLVGIALDHSTKFPNRVCATERQEKNYNICFEIFTVLMTLAIISASRIPFKYSDPPTNATRKYKSRINPPTNNDDDILAAQLNLPSLASGSKPAFGAKTNSQPELGAESKIFASTNREMPEWVTVLSYFKDLKCASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVAEETSHLAPHGVPSNPIPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Y4</accession>
    <name>T1H6Y4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6Y4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012466-RA">
        <property type="protein sequence ID" value="MESCA012466-PA"/>
        <property type="gene ID" value="MESCA012466"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_605935_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF63">
        <property type="entry name" value="EARLY GENE AT 23, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012466-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="452" mass="50992" checksum="3F23F3B9FAEB0B15" modified="2013-10-16" version="1">MSCRKKFFSGLTLRETIEYTALLRLPEKMPYVDKMSLVDRILEILELKPCENTLFGDYLNRGLSGGEKKRANIACELLTNPLLMLLDEPTSGLDSYSAISLMKVLKKYAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLAKMFGELPLVITLPTIYLTISYPMLGCCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGVQSSYVNCKNNGTEFIDYKDILNYQNSYSPLWLNTLILFSFFLVFRILGYIVLRYYRCPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA09</accession>
    <name>T1GA09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA09"/>
    <dbReference type="STRING" id="36166.T1GA09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000052-RA">
        <property type="protein sequence ID" value="MESCA000052-PA"/>
        <property type="gene ID" value="MESCA000052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453645_0_0_1"/>
    <dbReference type="OMA" id="IHENTHS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
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        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="415"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="521"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="602" mass="68364" checksum="337A10A11CC89B27" modified="2013-10-16" version="1">MMFNKNVEISFQVFTNDGMPPYICNPCRFTFEHSYRFKQMCKKADTALRQFPLTGRWPAKLERPEIPSELLPPPPPPPQEVYQNAPIILNSLPIEVKKPQIVSVVNKPSVKFMVKRESTSGKTPQTTPVKKRLLNKESFKILNKEAKGHSEPIMTTPVVQTTSDGNIEIVSKILETEEGADEPDPIKTAIPIQTNVFPCSQCERSFPLKQLLDMHMNRHLRERNFDCDQCDKSFFSKYDLGKHIMTHTGEKPFKCVACGKAFSRSTLLHRHEKSHTDIAKFLCVYCEKPFISKEEMEKHAERHKKHRPFACSYCEKSFAFKQGLERHESIHSKLQPYPCQYCNLSFSTPSKLARHLTAHAGTRPYPCKLCKKSYHLSHHLTRHMRSHKGNQGSYKCVECDEVFTTRDDLIYHSAFACEFCDLIFMTDDKLILHCASSHASEMAEYTDDNEGNSNTTVTNEAEGRDVVVQEFVIDEAAENTQNNEDDMTFENQLFEELMNDEPSTSTGGVKILSNIKLEKTSPEAKRKLSPKKKTISSPPEKKAKISPGSSKKEVIEMTIGDKKVKVQKISITKSEADAMAKAGRIQMKDGAMILSKGKAKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAF1</accession>
    <name>T1GAF1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA000207-RA">
        <property type="protein sequence ID" value="MESCA000207-PA"/>
        <property type="gene ID" value="MESCA000207"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266801_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13772">
        <property type="entry name" value="AIG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000207-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="12034" checksum="427A978B396AD9B7" modified="2013-10-16" version="1">MLKNSARNRDDYMKIIKVNQEENYHRITVDIKLDSGEILECETYEFDDLYMMEASNEDFKYGLLPSTTYVKVIVIGAIESNLSKKYIDXKNTPKFCNYSPNFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAM0</accession>
    <name>T1GAM0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAM0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000279-RA">
        <property type="protein sequence ID" value="MESCA000279-PA"/>
        <property type="gene ID" value="MESCA000279"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197019_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000279-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8921" checksum="CDE42A1AD7927C20" modified="2013-10-16" version="1">MYLEPTVPLYRGSPFKILGDVQFPDIQNDQRKSSQILGYSDKIDVIGRTNLDVEGIFLEIEKVASSYPNDLMVNGNKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAM7</accession>
    <name>T1GAM7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAM7"/>
    <dbReference type="STRING" id="36166.T1GAM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000286-RA">
        <property type="protein sequence ID" value="MESCA000286-PA"/>
        <property type="gene ID" value="MESCA000286"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002495_1_1_1"/>
    <dbReference type="OMA" id="ECCVESK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03868">
        <property type="entry name" value="M14_CPD_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000020">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF57">
        <property type="entry name" value="CARBOXYPEPTIDASE D, B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576765" description="Peptidase M14 domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="11">
        <location>
            <begin position="34"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="11">
        <location>
            <begin position="458"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000286-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="576" mass="66036" checksum="78FB0D0E787765FF" modified="2013-10-16" version="1">MKVIFVATLLLGSWIVNTNTFIIREDESFLDNPFYHSQEDIEELFDKLAKHFPQNARVVHVGKSAENRNLIALHISQNVQSRSLLTPMVKYIGNMHGDETIGRQLLIYLAQYLLFNYDKNLDVGSLVNSTDLYLMPTMNPDGFFRSQEGRCDSLPAYVGRINANGVDLNRDFPDRLDDERTILLKSRSRQPETAAMINWIKSEPFVLSANFHGGAVVASYPYDNSIYHRECCVESKSPDDRFFKLMAHTYSNNHPVMKTGKDCNETFKQGVTNGAYWYELNGGMQDFNYAFSNCFELTIELSCCKFPLANTLPEEWRKNKKSLLELIELAQVGVKGLVRDTNGYPISDAQIIVEHIEDKPIRTTNRGEYWRLLDKGTYTIYATAFGYHSSKPQVVEIVDNRAPVILNFNLKPEDSNDGISQIYSPYFFRKANYQHLVVERDSIGKDGKVEFLSKPEFVHHNSSAIESFVKRISRDYPNITRLYSAGKSVNGRELWVLEIAKNPGRHTFGIPEFKYVANMHGNEVVGREMLVLLTELIVENYKINDRITKLVDSTRMHFMYSLNPDGYEISSEGEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB24</accession>
    <name>T1GB24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000444-RA">
        <property type="protein sequence ID" value="MESCA000444-PA"/>
        <property type="gene ID" value="MESCA000444"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415779_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000444-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10412" checksum="95DA8620160D0641" modified="2013-10-16" version="1">MECPGFFISVSRGFGIPLLSFFCRVSNQQNRDDKRGQSSKFRQSIVRSLATTVKVPPSVTQESRDHGQSFNFHQSIAMSLQMTVQVPSFVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBJ0</accession>
    <name>T1GBJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ras-related protein Rab-35</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBJ0"/>
    <dbReference type="STRING" id="36166.T1GBJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000622-RA">
        <property type="protein sequence ID" value="MESCA000622-PA"/>
        <property type="gene ID" value="MESCA000622"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_24_2_1"/>
    <dbReference type="OMA" id="RDANICF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000622-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11935" checksum="A82F110E53F09771" modified="2013-10-16" version="1">RSITKGVGKSSLLIRFSDDTFSGSYITTIGVDFKIRTVIIDNKRIKLQIWDTAGQERFRTITSTYYRGTHGVIIVYDVTNGESFANVRRWLQEIQQNCEVVNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBS8</accession>
    <name>T1GBS8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBS8"/>
    <dbReference type="STRING" id="36166.T1GBS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000716-RA">
        <property type="protein sequence ID" value="MESCA000716-PA"/>
        <property type="gene ID" value="MESCA000716"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_16_0_1"/>
    <dbReference type="OMA" id="YLMWASA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF1">
        <property type="entry name" value="LD38433P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000716-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="272" mass="30905" checksum="38720DDCD0E38B7C" modified="2013-10-16" version="1">MRGCTFRTGKEQSTMVSAGPMSRHAEDLLPLLKVLVCDEKKTSLRLDENVDLKKLRYFYISENGVVASTPVNSETQNQMKKVTRYFEELTEQDVVKTKLPGLEKSKNMWRYWMTQEPANFNRLMGNGDEINPIVELLKKLVGQSDFSMAGIFGLIDGILPAEKEKIVKDVTQKLEEALNELLGDDGILFFHSNPRTAPFHYHPLFLKFNDFHYFSIFNVLKVPVTQVPLGLDSNGLPLGIQVVATKNRDRHCIAVAQELERAFGGWVPPFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBY9</accession>
    <name>T1GBY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBY9"/>
    <dbReference type="STRING" id="36166.T1GBY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000781-RA">
        <property type="protein sequence ID" value="MESCA000781-PA"/>
        <property type="gene ID" value="MESCA000781"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_23_6_1"/>
    <dbReference type="OMA" id="FRMPTRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="58"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000781-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14294" checksum="0A4BFCED024113F0" modified="2013-10-16" version="1">MVIDPELEAIKARVKEMEEEAEKIKQMQSEVDKQMAGSAAGAATVPLTAEEKAEIDSRSVYVGNVDYGASAEELEAHFHGCGTINRVTILCNKADGHPKGFAYIEFGAKEFVETALGMNETLFRGRQIKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC79</accession>
    <name>T1GC79_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC79"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000881-RA">
        <property type="protein sequence ID" value="MESCA000881-PA"/>
        <property type="gene ID" value="MESCA000881"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815362_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000881-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7831" checksum="4F60D6386EFB7045" modified="2013-10-16" version="1">MDSGIISLQSKKKYNLMQESNEHRHCEDTLLQLSPPQRHCNLLLTSLYKLHQKHCNLPFPPINSDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCE6</accession>
    <name>T1GCE6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCE6"/>
    <dbReference type="STRING" id="36166.T1GCE6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000957-RA">
        <property type="protein sequence ID" value="MESCA000957-PA"/>
        <property type="gene ID" value="MESCA000957"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_2_1_1"/>
    <dbReference type="OMA" id="NCERRKY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000086">
        <property type="entry name" value="transcription factor E4F1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002467">
        <property type="entry name" value="Zinc finger protein 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000957-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="284" mass="32020" checksum="583478DFA8E87100" modified="2013-10-16" version="1">MDSMSQTVVLHPHEVETTELSIKKDSGHHPVKKRNQSHVTKCQKCNGTGIIYVGGTNKTCIKVETPKPFTCNICGGTFSRYSSLWSHKKLHSGEKNYKCTICGLAFAKAVYLKNHARIHTGEKPYKCQTCGMQFSQSPHLKNHERTHSGEKPYVCEVCDKGFARHATLWNHRRIHTGEKPYKCSICSSAFSQAAHLKNHAKVHSGEKPHKCDICSAAFGDRFALKRHRGIHEKYGRTNCTEKQEDTDDAMDDADQSGLMYVVEKQELQDMDDDAQQEIEIISGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCH9</accession>
    <name>T1GCH9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC-type glutathione-S-conjugate transporter</fullName>
            <ecNumber evidence="10">7.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.3" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02184215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCH9"/>
    <dbReference type="STRING" id="36166.T1GCH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000993-RA">
        <property type="protein sequence ID" value="MESCA000993-PA"/>
        <property type="gene ID" value="MESCA000993"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_1"/>
    <dbReference type="OMA" id="IRARITM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577263" description="ABC-type glutathione-S-conjugate transporter" evidence="13">
        <location>
            <begin position="17"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="98"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="342"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000993-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="574" mass="64315" checksum="6A05699EA4324576" modified="2013-10-16" version="1">MVFFAILSLYLGATRASKVIHAKSVSNLIRNPMSFFDSTPLGRILNRFTADVDEIDEELPSTIHALLNCLYLYFTNDYSNVPKFLVQPFGLLNAALVLHLKLLFNTLRVPMSFFDTTPTGRILSRFSKDIDAVDVTLPRIIIDCLWCLFEVLATIVVISISTPIFMAAILPIAVLYYLAQRFYVATSRQLMRLESVSRSPIYSHFGETVTGVSSIRAYNVQDEFIQENDDKVDRNQVCKYPNIVANRWLAIRLEMVGNLIILFSSLFAVLGGQENAGLVGLSITYALQITQTLNWLVRMTSDIETNIVAVERIKEYGETKQEAPWVLENSKLSPDWPEQGRVQFTDFKVRYREGLDLVLRGISFDVNGGEKIGIVGRTGAGKSRGKIFIDGIDISTLGLHNLRSRLTIIPQDPVLFSGSLRLNLDPFDTKSDEELWKALELAHLKTFVKSLTAGLNHEISEGGENLSVGQRQLVCLARALLRKTKVLILDEATAAVDLETDDLIQKTIRTEFKECTVLTIAHRLNTIMDSDRVIVLDKGEITEYASPTELLQNKSSAFYSMAKDANLVSGDKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCJ6</accession>
    <name>T1GCJ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCJ6"/>
    <dbReference type="STRING" id="36166.T1GCJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001011-RA">
        <property type="protein sequence ID" value="MESCA001011-PA"/>
        <property type="gene ID" value="MESCA001011"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2747387_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001011-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7980" checksum="55763C2747CC170C" modified="2013-10-16" version="1">MGFLDMTDRRATKISVMILGGALFSILMAYMVFGDNNDEQGLKTLRMISILFRHGDKNPSGFYPTDPHAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCU9</accession>
    <name>T1GCU9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02064192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCU9"/>
    <dbReference type="STRING" id="36166.T1GCU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001122-RA">
        <property type="protein sequence ID" value="MESCA001122-PA"/>
        <property type="gene ID" value="MESCA001122"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="OMA" id="MAIIVSC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="15"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001122-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="483" mass="52442" checksum="A6BE7D9366C6935A" modified="2013-10-16" version="1">MTHGNNAKSLPQILAALAAAGGALVAGTVLGWSSQIETELVTNDKYGFDISEEQFSWFSSITNLGAGFMCIPTGYLCGLIGRKFTLLGLAIPLILGWSLVTFANENGMILAGRFFLGMGSGGFCVAAPMYTGEIASKNIRGTLGSFFQLMITLGILVAYILGAYCSLFVYNIICFVLPFIFIITFAFMPESPRYLEAETKAALQRLRGDSYDITAELNELKSEDEKSKNENVNAFAALGKKVGIKSLFIGLGLMFFQQLSGINAVIFYSGSIFKDANTGMSMTICTIIVGIVQVVATFVSTLIVDKAGRKLLLIVSAVSMAICTICLGVYFYMQKQDEESVKDIAWLPLASLILFICMFSIGFGPVPWLMMGELFSHEIKSIASSLCGTTNWMLAFIVTKAYGPLKDGIGGGPVFWIFSGISLIGGVFVFLATPETKGKTLAEVQAHLEGSSANKSNPQKLKRTTIFVKYLKSVYFFYFKLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCY9</accession>
    <name>T1GCY9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02091365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001163-RA">
        <property type="protein sequence ID" value="MESCA001163-PA"/>
        <property type="gene ID" value="MESCA001163"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199961_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12316" checksum="511F61A7DA4F149F" modified="2013-10-16" version="1">MLNCQKAVGNHSADHWAANILSPSDGTIEMSPSLGVVVNQLKSTVDYHNKQKSNYKNSLDLTVRTNYEEVCERMLEKQEESVKVSRSQNNVFFMDSFRFLYGTSDFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDR2</accession>
    <name>T1GDR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001456-RA">
        <property type="protein sequence ID" value="MESCA001456-PA"/>
        <property type="gene ID" value="MESCA001456"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126756_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577250" description="Farnesoic acid O-methyl transferase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001456-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19316" checksum="13E02B8F5E85D959" modified="2013-10-16" version="1">MNRVLIISAVLTFTCILLANGGSFNNCKVNIFINTTDIKENYPQENELHRFRLYVNGRLPFIKLTSSGQTITFGEYKNETDFAIFREEDDYFKSLEPCSYQDNIHVFADSKFVEVWFILEKTGHFSIIAGNMDNGFALSCNTGFNFTQTSEDRLYTREPVLYDCGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE23</accession>
    <name>T1GE23_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001580-RA">
        <property type="protein sequence ID" value="MESCA001580-PA"/>
        <property type="gene ID" value="MESCA001580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052280_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="14156" checksum="F837E9DDED484414" modified="2013-10-16" version="1">MFALVRALHERGSNLLHEVELLILYSKRSRGHVWLLVHNSLQGTIIKPQRRRKTSYLLTLKILIIFIIIDGQHMIKNKNKLSSKHSLQNEKQFKRFITGKHQPGNRETTTWKLKYSSLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE54</accession>
    <name>T1GE54_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169392">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001612-RA">
        <property type="protein sequence ID" value="MESCA001612-PA"/>
        <property type="gene ID" value="MESCA001612"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944355_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001612-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="7116" checksum="20CC2C0B193ED979" modified="2013-10-16" version="1">MAKGWRDAEHDMAVEIWSLKVIAVRIEVNNFSDPNEHCSRWLNHLVFLWSFTSLWMVLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GED5</accession>
    <name>T1GED5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02086455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GED5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001696-RA">
        <property type="protein sequence ID" value="MESCA001696-PA"/>
        <property type="gene ID" value="MESCA001696"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3392736_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001696-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="32" mass="3674" checksum="2F6F9BC714726C97" modified="2013-10-16" version="1">MNLNHLPVLHSQSLNLCHFSQSNTNPQFPKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFF8</accession>
    <name>T1GFF8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HDAg domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166925">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFF8"/>
    <dbReference type="STRING" id="36166.T1GFF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002099-RA">
        <property type="protein sequence ID" value="MESCA002099-PA"/>
        <property type="gene ID" value="MESCA002099"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_480295_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037517">
        <property type="entry name" value="HDAG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052828">
        <property type="entry name" value="NELF-A_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328:SF4">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A NELF-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51838">
        <property type="entry name" value="HDAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDAg" evidence="2">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002099-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="568" mass="60762" checksum="FEB0C621FC795799" modified="2013-10-16" version="1">MLPLECLYLNKNALVSVVGQQSNPVKHFTLKRKPKSTNLRTELLQKSSDAQSSLKKTTAPTIPLRSRGMPRKMTDTTPLKGIPSRVPVSGFRSPNSTQNPSRPNLIRTPGGRKDGGIKIIELSEQPLGFAAAKKLYSTATIKEEIPDEKPQADGISCSESIMSTTTSSTPISTTSIINSNKKFIKEETFVITKKEIEDEDTDMMDNSKNSLSPTKCANLPFNSKQIKSEKGVTSTSMKSPEIIKTMTTLTTENLKHKNEQQKPSTSDPLINLPNNISVKITPTKIQNQTLVSGSSLIIKPTTPTTIKSIPIANQIKTATSSTGGPVIISQTIIQPAASSKSGGSIASSSSGFVLPSNASIIRTTTAGGQQKIILAHSSQSNTVPSLTSLSQQSPPNTQTKIILKTSAGGQLQPLVVTSRAQQNNPSGSQSNRAVTIQPASQAAQAQHLLLQQQQQKQDPQNFTQVVMTSTSNNSTSGKGKTLYIPQKAIVLQNIGGDMYKQIPISNVSNLQTLTATSSNQPPNLVQTTLTTSANKSAQQQIPALIPSSNLPVVIQQQPQSMQTIISAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFI2</accession>
    <name>T1GFI2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 40</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFI2"/>
    <dbReference type="STRING" id="36166.T1GFI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002124-RA">
        <property type="protein sequence ID" value="MESCA002124-PA"/>
        <property type="gene ID" value="MESCA002124"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_18_23_1"/>
    <dbReference type="OMA" id="WHILEPE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000116">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="42599" checksum="4253E516A1373231" modified="2013-10-16" version="1">MASKSRIHKSNLNPVFHKRNKDEDCAELTKELVKSAKKTGILCLSNKSLATIPDKVWHILEPEKPVEVTLDSLCENDEDELWWNQKELTNLDLSSNSLTSISPKIQNLYSLTVLNDLSNNQITSLPGGMGFLVRLTELNCSYNEINELPADFVNMRSLQKWDMMHNNLKSLPEDIGQLRKLECLYVQHNDIEELPIFDGCEQLKELHISNNFIKEIPADFCEKLPHLKILDLRDNKIEKLTDEIAFLQNLIRLDLSNNSINSLPNCLSTLAHLQSLQLEGNPIKSIRRDILQCGTQRVLKTLRDRAQENSQDMALVKKPIIGESESLPDRYKIRKNRCLSVTMMGLSEIPEQVFTDALEENAYMVDFSKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFM9</accession>
    <name>T1GFM9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002174-RA">
        <property type="protein sequence ID" value="MESCA002174-PA"/>
        <property type="gene ID" value="MESCA002174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909080_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15050" checksum="305B01B7D67ABE2B" modified="2013-10-16" version="1">MLPAGMGTSLVSAISSSSNGSCLLYVGGHILYSSWLYLNSPSKQTAQKCSKQSRLLLLSFLPHSISWAFASQMEKFKLIKLLYQNIDLRNIDFDFEQVKILDVESTRLKRNIPEMINIKLEEHTAMNDLIPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG42</accession>
    <name>T1GG42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02032122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG42"/>
    <dbReference type="STRING" id="36166.T1GG42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002348-RA">
        <property type="protein sequence ID" value="MESCA002348-PA"/>
        <property type="gene ID" value="MESCA002348"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027239_1_1_1"/>
    <dbReference type="OMA" id="NIWENEQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="62"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="331" mass="39628" checksum="A4F01A6FE1BB0F9C" modified="2013-10-16" version="1">MQSESKYFICTEENEEFNNLRLKDSSYLPQILQWDQEKLTKTKRMYPFEKLDYFHEFKVREDDIWIVTNDRCGTTWTQEMTWLILNNLDFDQARSVPLETRSPFLEQCLNSRLPTPSNFNFESVPSPRLIKCHLPMQLLPKEIWTKKPKIIYVVRDPRDAFVSSFYLTKLFMGVNSSLSLDMEDYIEKLIKFSCFWNHVLGFYKLRKQENILFYSYEQMKKDLKSVVLRVCNFVGINYKDEEIEKLLEHLDFKNMKDSESCSHKLEIEQVHKNLQLKDEDIDPSMKFIRKGKVGGHRSELSPEILKKFNIWENEQLEKYGLTLKDILFSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG72</accession>
    <name>T1GG72_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02116019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG72"/>
    <dbReference type="STRING" id="36166.T1GG72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002382-RA">
        <property type="protein sequence ID" value="MESCA002382-PA"/>
        <property type="gene ID" value="MESCA002382"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1046926_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002382-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="266" mass="30558" checksum="65C65E8A2802C6E4" modified="2013-10-16" version="1">MRLNMCTIPPTYLKVNKFTKAFQAIVDAYGVPNYKELNPAPYTIITFPFLFAVMFGDWGHGLIVFCLSLYLIFNEKNIAKADLNDIFKIMFDGRYIIMMLGAFSIYTGLIYNDIFSKDMKLMESSWRTDLYNITTIGSLREELALNPITDYVGTPYIFGFDPMIPLSGETSILIKNSIKMKMSIILGFFQMLMGIVLSAINAIYYKSYISLFLVFLPTMTFFCSLFGYLLSYMNISLLYNYSQLLSLIFKLVLPVNRKGSQKSQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG84</accession>
    <name>T1GG84_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002396-RA">
        <property type="protein sequence ID" value="MESCA002396-PA"/>
        <property type="gene ID" value="MESCA002396"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963410_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002396-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="7201" checksum="77A2E0BA27858DB6" modified="2013-10-16" version="1">MKSKKFYLPPFPQGGMLRKRNAGHFHFIMKKFALKKDLMYMDILMRHKYKMYILCTTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGD7</accession>
    <name>T1GGD7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Peptidyl-prolyl cis-trans isomerase E</fullName>
            <shortName evidence="10">PPIase E</shortName>
            <ecNumber evidence="4 10">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cyclophilin-type PPIase family. PPIase E subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4 10"/>
    <dbReference type="EMBL" id="CAQQ02195522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGD7"/>
    <dbReference type="STRING" id="36166.T1GGD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002453-RA">
        <property type="protein sequence ID" value="MESCA002453-PA"/>
        <property type="gene ID" value="MESCA002453"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_27_0_1"/>
    <dbReference type="OMA" id="KIVIYAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12347">
        <property type="entry name" value="RRM_PPIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000010">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016304">
        <property type="entry name" value="PPIE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034168">
        <property type="entry name" value="PPIE_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001475">
        <property type="entry name" value="PPI_cyclophilin_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="13">
        <location>
            <begin position="131"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="289" mass="31876" checksum="CAC531E586BC3BE9" modified="2013-10-16" version="1">MSSTNDKRTIYVGGLADEVTEQLLNAAFIPFGDIADIQIPVDFESQKHRGFAFVEYECADDAAAAIDNMNDAELLGRTIRPIWADDEWLQKHAGATLEDEEGDEKEESSKETTASVPATISKPEQRNPQVFFDIRIGGNEAGRIIMLLRADIVPKTAENFRQLCTHEQGFGYKGCVFHRVIPDFMCQGGDFTNHNGTGGKSIYGKKFADENFTLKHTGFGTLSMANSGSNTNGSQFFICTTKTDWLDNKHVVFGHVISGADVVRKMERCGTKTGTPNQKIVIFSCGELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGQ7</accession>
    <name>T1GGQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02198073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GGQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002583-RA">
        <property type="protein sequence ID" value="MESCA002583-PA"/>
        <property type="gene ID" value="MESCA002583"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716251_0_0_1"/>
    <dbReference type="OMA" id="RKIRQFN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="10">
        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="11">
        <location>
            <begin position="349"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002583-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="48255" checksum="BF3FDB554D4D2438" modified="2013-10-16" version="1">MTFLESNNICTEENFIIFIAEPEKYADKANEIISKISSDLEMEDANEFRKRIPYNTTDPQIIEQQKTLLEFLISNGICTEENFEIFIADYDNRKNEAAEVMNQFVASESTDGSELSSNVEMEGVEILPPLNQEKEQKLYPLFYADKAKEAYKKLSFETSRKIRQFNGGFGANQYQIXAKEAYKKLSFETSRKIRQFNGGFGANQYQIDAGQKEFGAKQCKDCGLIYTVHEPEEEKLHREYHDNLYILRFKGWIDEVVVGRFPEWSPDGRIIKLTHNDPIKRRQRIHEVLRVVVDKELGFSSYILPQEYVAYFAISKNKIIGMCLVQPLEKAYKYLCIDGVDCCSLESYDVKCGISRIWISPFYRRLKVGKKLINAVQSNTFLGNKLSMTDIAFSAPTEDGKRFAKNVSQKEDFFVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH19</accession>
    <name>T1GH19_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02087139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02087140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH19"/>
    <dbReference type="STRING" id="36166.T1GH19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002702-RA">
        <property type="protein sequence ID" value="MESCA002702-PA"/>
        <property type="gene ID" value="MESCA002702"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1534297_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002702-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="19361" checksum="9EEB316AD8073700" modified="2013-10-16" version="1">MQEGAVLAKLKTKWWKEKRGGGACVDDDSDGGATPLEVANLAGVFLVLYVGTVIAFICGIVEWLFVIKAEAKKEQVPFCVKFKEEFKFMTTLSETTREVEPEEKEESSGSSSSTRASQRPNPNQSPKRDKAPHPVLPQLIHLPPNAKLSLKICGLEMFPMSEQIRNLKKAQGNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL2</accession>
    <name>T1GHL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02095727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL2"/>
    <dbReference type="STRING" id="36166.T1GHL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002919-RA">
        <property type="protein sequence ID" value="MESCA002919-PA"/>
        <property type="gene ID" value="MESCA002919"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_286093_0_0_1"/>
    <dbReference type="OMA" id="CALYSAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13275">
        <property type="entry name" value="PH_M-RIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052223">
        <property type="entry name" value="Actin_Cytoskeleton_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039597">
        <property type="entry name" value="M-RIP_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271:SF1">
        <property type="entry name" value="PROTEIN OUTSPREAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="170"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="473"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="586"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="869"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002919-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1081" mass="123798" checksum="6CC168D7E8107F0E" modified="2013-10-16" version="1">MDSRVVRVNHMLTTSREELEELRQEILSFSEKYSIKCVENAALEEKLRSANQKLKNFQQMQQLELREPDYPLSSSQNLVTLRPKSLPLASNSTPAIVSAIVKKIPPVQQQSNEITNKSSPNRLQQHLRKLQKLHHERGDPDGGCNIDELSTNYLSSNSELRVCLATEDVINSKKGWLMKQEIGSGEWSKHWFSLRGAALFFYRDPVSEERGVLDGVLDVNSLTNVREINSPRNYAFQLTTWDKRRIILSGLSANSRNNWITVLTNVSGLKQQQQNSQQIDDIKKKDIQEKEVIIKDNTSDDIKREIEKDFIKAQRNQEIILNSITNNSNVPATEIKSTNTTPAVTPITPKSIMFSSDDEYRTASEGGRRDSADWGSPLSPSPPVPLSLFRSKEKSRPRTNSSPRLSKEVDHHLQVQGAAHVGSEDIPQIHPVQEEINDNDLELRLGLAEKERDLLKSEAREREVRMSELLTTLEKTEHELTSKVREMELARDKFSNELEAANRSADKIINHMTFELDDSHKKIKSLEDRLARGIEENEELYKKIREYEGCNPSGFNNLLSRTKIKRMDSLSDLTNITDIDPFCLDRNVLADEYNELKSRFEKAVNEIKAMKKELKDSQNDYDSLEISYASLKQDFERKQCNDKSQLKMMAERIQDLTLKYSGSERQVRTLKQKLAKSERRRSLSLKSKDQLTVSKEFEIKINDLETKIEEIEKMNALKRSATDSKIACKKTSNLRRKSLEGSSSTTLCILWFEKRYDSVHNNTLDSELMSITSSISSSPNISEHMIDRLRSLENILVSTKDRIEQNLQQIQTLRSSRTRRSVSPISDRKDSFKVIEKSLTEVVKVLRESCDQCVIENTLQESNPIKIALVQLENQLRSKLNNLLNQRRVLRDRNELTQNKDLCLIAERIAFESVCFGMLRDSLMTDQIYADNIGNNEIIETSHHISQLKARLSGKCSILKSIDSLEVLSTILAKRLMQSSYKSNKNTITEINRPVDPNLLENIIRQQNEINLIIKRYKNNTLEKLASGLAAETLSYISSKDLVQGAVQEAWRQAQEAVNAELIQSEISHIMLKNAERLENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHP9</accession>
    <name>T1GHP9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02116652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHP9"/>
    <dbReference type="STRING" id="36166.T1GHP9"/>
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        <property type="protein sequence ID" value="MESCA002957-PA"/>
        <property type="gene ID" value="MESCA002957"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041204_0_0_1"/>
    <dbReference type="OMA" id="ERTCINE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dumpy_DPY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22963:SF39">
        <property type="entry name" value="DUMPY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENDOGLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="DPY module"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="242"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002957-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="453" mass="50842" checksum="F23EF78A668EC88F" modified="2013-10-16" version="1">MELHFVHVDQTILVNLLIVDQNNPCESFCGAFSKCIVKNHIPVCYCIDNYEGNPYLQCSRKPERTEISHNIQESPCECGVNAYCENNICKCLDNFYGNPNAECKPECVYDNECGFDQKCVNMKCLQSCSLIECGENANCKVNYHISACVCNENYYGDPYSRICSCLDGYVGSAPACRPECLTDNDCSSERTCINEKCVSPCSICAYNADCSVIRHKPICSCPEEMSGNPYSYCWPKPYIKQEDDVCNPSPCGLFSTCTNNNNVPECKCLENYIGRPPNCRPECTSDFDCISTQHCRNQKCENLCSEGICGENAECLAKFHRVSCLCLPGYTGSPYIQCTKPRSEVSFLTPCEKYPCGMNAKCLNINGREHCVCLENYFGNPYVQCNPECMLNSDCSNNMVCIQQKCQDPCIGLCASNALCFVRDHIPRCTCQPDFDGNPYELCYPKRQSKVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI91</accession>
    <name>T1GI91_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI91"/>
    <dbReference type="STRING" id="36166.T1GI91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003160-RA">
        <property type="protein sequence ID" value="MESCA003160-PA"/>
        <property type="gene ID" value="MESCA003160"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161228_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:double-stranded RNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070920">
        <property type="term" value="P:regulation of regulatory ncRNA processing"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF3">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003160-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12482" checksum="8DE4393603B0355A" modified="2013-10-16" version="1">MSRYGDVKDSSVQSINNSYSQKVAQFHKTLKNSSGPKLNNLQLVCLKDKKLDAVKFLEEISQEQQFEVTYVDIEEKTFSNSSQCLVQLSTLPVAVCHGTGANTKRPRQMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIQ6</accession>
    <name>T1GIQ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIQ6"/>
    <dbReference type="STRING" id="36166.T1GIQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003341-RA">
        <property type="protein sequence ID" value="MESCA003341-PA"/>
        <property type="gene ID" value="MESCA003341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_2_1_1"/>
    <dbReference type="OMA" id="TICKMAF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="19833" checksum="3DD04C6506E65114" modified="2013-10-16" version="1">MEIVSMEVEEDPSKKGQFRCENCSRTYKRLTDYNTHKKTCSTTKNVQCTICKMAFFTIFGLRQHMRKLHALVKQYVCEECGRNFYVLEDLEKHRSNHKEQQCTECGKTYANIKQLRRHLDCHSSTSYVCTVCGISLNTRHTLNHHMKKHAEILNQFVIIVVKSLKCPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ90</accession>
    <name>T1GJ90_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02001012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003534-RA">
        <property type="protein sequence ID" value="MESCA003534-PA"/>
        <property type="gene ID" value="MESCA003534"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707612_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003534-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8888" checksum="69D27D2A11A08561" modified="2013-10-16" version="1">MELTLEEYTAFSTFQVEQLSGMERNKKTRAAQEAYKKKRKKVVKLTRQKKRRFGSNQFDEMEILGNRYYVRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJN4</accession>
    <name>T1GJN4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003684-RA">
        <property type="protein sequence ID" value="MESCA003684-PA"/>
        <property type="gene ID" value="MESCA003684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3093466_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="6105" checksum="B50F45D72C35BA98" modified="2013-10-16" version="1">MCNANKNFKETENFVGMYSFRQPQLLLLSPDSIKEVLIDKFHSFHDNDISKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJN7</accession>
    <name>T1GJN7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003687-RA">
        <property type="protein sequence ID" value="MESCA003687-PA"/>
        <property type="gene ID" value="MESCA003687"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888332_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003687-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="6815" checksum="141C004C4EB7AC0A" modified="2013-10-16" version="1">MHGYGKFQQILSEDKVDISPPLEFHGGKTRGGSAYFPRVPGGRSSSNQQQTKSGATKDYTIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJW4</accession>
    <name>T1GJW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJW4"/>
    <dbReference type="STRING" id="36166.T1GJW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003771-RA">
        <property type="protein sequence ID" value="MESCA003771-PA"/>
        <property type="gene ID" value="MESCA003771"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867438_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003771-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="15660" checksum="6EA9A7D6804E003B" modified="2013-10-16" version="1">MRGQTALAQKFICCVADILEKVVPLVTNPSESFLASLEEHLMFLVISFNQAVVSSCISCLSALVNKITKNYKLIRDCFVRFYKQMVKSKEHVLANPTVTIDKIYTPIFRRSLFTIGILMRYFDFKSRRVLGIDEGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK22</accession>
    <name>T1GK22_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurogenic mastermind-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
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        <lineage>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189245">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA003833-RA">
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        <property type="gene ID" value="MESCA003833"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600344_0_0_1"/>
    <dbReference type="OMA" id="NTGMKMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003833-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50824" checksum="38283F41E3CEA59B" modified="2013-10-16" version="1">MGVSHDMITRPIEDLDNGPDNFEPPHKLPHHHNGQQHQQQQQQQQQQQQQQNSSSTTNNTNNNNGNANTNNNNGGTENITKFSLEILEKAQPNGQFPDFPDLMGDDTNEDNNTFNDLINDLQDFNPDFLQGFEEKPLLDIKSEGDVKMELDALNIKTEPSQQPNHMSQFQNAFPQETNMNKMRQCTNNAPMSELSPAAQTLNKWPNNTSTKILLGPPQQPQNHQMIGNGNRFNDFSTFGNDFLGASSGNGAQQTSAQNQPLPMQPQKTGNGQPNQNFVDIKQEMFYSSQNEYDLKRLQQQQQQQQQQQQQNMQHMGHPGMQKMGVPPGPGNFQNKPQQQQQYSPFSMQSNNSAQQQQNPQSFISQQRNGPIIGNGGNTNIPGAQQSQGPNPQQQQQQGNTANSRQATNTQQNPNATNNAPQNNGGNGGATLQMKQSQQLHISQQNGNHGIQFLCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKF8</accession>
    <name>T1GKF8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034598">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034599">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKF8"/>
    <dbReference type="STRING" id="36166.T1GKF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003981-RA">
        <property type="protein sequence ID" value="MESCA003981-PA"/>
        <property type="gene ID" value="MESCA003981"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926136_0_0_1"/>
    <dbReference type="OMA" id="CECREHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028426">
        <property type="entry name" value="Huntingtin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10170:SF10">
        <property type="entry name" value="HUNTINGTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10170">
        <property type="entry name" value="HUNTINGTON DISEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003981-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="301" mass="34704" checksum="B1517DADD2352910" modified="2013-10-16" version="1">LKKLHQAQLLLCESSNVFNHPNLERDLSSRQNDNFIGTHNFWPNQYDLYYIWHQMEQSSQFQQSVDEGSEDFRVSDDFEAGTKIMYYCERSGIDFKSSAQVVFDVLSELLQTNPSLSLPVLVNFVEICECREHIEWIKDTIQRIQITIPVDDTISHQHIIYLMCKTQAILIPTLSEVKQLCAQITFYLKSSHIFVRNATLCGLLSLLECCTKTNTTIGKLSEELTALREVSTAYINFHISNSSIKCSSVHTRLVWTLNFCLIEWTSKFVPDCKLISTTIPIAKDILRTTNDEHLYLIVLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKW7</accession>
    <name>T1GKW7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004148-RA">
        <property type="protein sequence ID" value="MESCA004148-PA"/>
        <property type="gene ID" value="MESCA004148"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545210_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9575" checksum="A6FD52E02A625BD8" modified="2013-10-16" version="1">MDRPFNRGREEIRGYPMNDSHQLCSSSSIKDMRGNLGIPTHLSTKGGLLILKRRIWEDFHLKTKPEPQNSENLSIQKPDRPCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKZ2</accession>
    <name>T1GKZ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">L-lactate dehydrogenase</fullName>
            <ecNumber evidence="3 9">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>(S)-lactate + NAD(+) = pyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="3 9"/>
    <dbReference type="EMBL" id="CAQQ02161970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GKZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004175-RA">
        <property type="protein sequence ID" value="MESCA004175-PA"/>
        <property type="gene ID" value="MESCA004175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_0_2_1"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05293">
        <property type="entry name" value="LDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000018">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011304">
        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="10">
        <location>
            <begin position="45"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="174"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="333" mass="36594" checksum="D7B09214ECFBB7DF" modified="2013-10-16" version="1">MQNLQQQQSHRRRYRSSWYGXXXXXXXXXXXXXXXXXXXXXXXXXCAFSILTQNVSNEICLIDVSADKLQGEMMDLQHGSNFLKSPKVTAGTDYALTAGSRLCIVTAGVRQKEGETRLSLVQRNTDILKHIIPKLVEHSPNTILLMVSNPVDIMTYVAWKLSGLPKNHVIGSGTNLDSSRFRFLMSQRFGVASQSCHGWIIGEHGDSSVPVWSGVNIAGVRLRELNPTIGTAQDPEKWNELHKQVVDSAYEVIKLKGYTSWAIGLSVSNLASAILKNTNSVVAVSTLVQGEHGIGHEVFLSLPCVLGSNGVTHIVKQILTEAEIKQLQNSANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL06</accession>
    <name>T1GL06_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL06"/>
    <dbReference type="STRING" id="36166.T1GL06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004189-RA">
        <property type="protein sequence ID" value="MESCA004189-PA"/>
        <property type="gene ID" value="MESCA004189"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237701_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF131">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004189-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="224" mass="25244" checksum="344F614652190B8F" modified="2013-10-16" version="1">LLGIFPKQLPKREKRFHSEVPRVLRVNEICEEDNESHLAKCYASNSTLDKVVPETKLPSMKDFPKALMRLLKNRLLMFNNISAVFYILGASGYMTFLARYMEVQFLKSPQEATILIGPLTLFGMVLSIMLSGLVITKKKPRPSIVLFWNVFVGVLYMVGQVAYFFLFCEANTSTFLQGSALNMSMPCNLECQCDGVAYNPDVTPGTKSINDMKIVTALKIQTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLC4</accession>
    <name>T1GLC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 28 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Component of the ESCRT-I complex, a regulator of vesicular trafficking process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ESCRT-I complex (endosomal sorting complex required for transport I).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the VPS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLC4"/>
    <dbReference type="STRING" id="36166.T1GLC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004324-RA">
        <property type="protein sequence ID" value="MESCA004324-PA"/>
        <property type="gene ID" value="MESCA004324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076417_2_0_1"/>
    <dbReference type="OMA" id="CDEFPTV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1130:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 28 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.200:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 28 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007143">
        <property type="entry name" value="Vps28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017899">
        <property type="entry name" value="VPS28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037206">
        <property type="entry name" value="VPS28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017898">
        <property type="entry name" value="VPS28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038358">
        <property type="entry name" value="VPS28_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03997">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017535">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140427">
        <property type="entry name" value="VPS28 C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51310">
        <property type="entry name" value="VPS28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51313">
        <property type="entry name" value="VPS28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0967">Endosome</keyword>
    <keyword evidence="4 7 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS28 N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="VPS28 C-terminal" evidence="9">
        <location>
            <begin position="112"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="24643" checksum="70D22C6DA8BCA110" modified="2013-10-16" version="1">MQENRPELYEEVKLYRNAREREKYDNQADLFALITTLQNLEKAYIWDATTPQEYTAACSKLLVQYKVAFRQVKSDEFPTVDAFVKKFRLDCPAALERIKEDRPITIKDDKGNTSKCIADIVSLFITIMDKLRLKIKTMDELHFELKDLVDTMHRLSLIPEDFEAKKKVETWLATLNEMQASDELTDSQVRQFLFDLESSYADFNKLLHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLI7</accession>
    <name>T1GLI7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02097263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLI7"/>
    <dbReference type="STRING" id="36166.T1GLI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004391-RA">
        <property type="protein sequence ID" value="MESCA004391-PA"/>
        <property type="gene ID" value="MESCA004391"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_687515_0_0_1"/>
    <dbReference type="OMA" id="NCTIEDY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004391-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="401" mass="45242" checksum="CD96CC14F3F49B8E" modified="2013-10-16" version="1">MMKYDNPITPGTRRRNDVIKCLNDVWRPKSLPPSSSGEESSPKSNTSPKSPISKAFDFLPWPSRNKGQPNSKSNNTATQSQNEQNYHKNIFRNSGGIIRDILSDKLNKDKGNSGKKFKYTSRNKSLDINELINNCTIEDYGNKNYNNENNDLINSSNNDNNNNNNNNHSDNKKHILFNNENVVHIFNGKKSPCGEKSNGTSIIKARLKLQKMNSIPTPPLPPSSNQKSILTRNSVPSPGRASLPPNHPALNYQRSKSEIVIENRSMNEKDSERPKTVKPRKKLSFREPIASHLRMKYQDGRFQVESEKNHYLNDGGGGKGNGRPAFDNASTCESDLESQAMRVVSTVGQAFEVCHQFNLQKNSLDNNDETSDLSFELYDSERRSQNSDDDSSKKVIFITYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLL2</accession>
    <name>T1GLL2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLL2"/>
    <dbReference type="STRING" id="36166.T1GLL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004422-RA">
        <property type="protein sequence ID" value="MESCA004422-PA"/>
        <property type="gene ID" value="MESCA004422"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_928402_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030217">
        <property type="entry name" value="NXF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662:SF22">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004422-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="300" mass="34564" checksum="F488273344E7476C" modified="2013-10-16" version="1">MDRLEADEVLRFQCYSSIYLKTRDAVCYSGTSKFNRELAYRPDSWHQVKVMHNGDYSKEEILSAFFEIMRDTTFAPVSYYVGESLDSFVFPSSNKIVVKNMFRKSLKLTMPSGKPLHFSVTLGVAKTSSDQISLKKVLWKILDSELNKLRHYQGVKDVLFLEKLAIHPECRDICVSLGNPGTLQTLLEILITCFSKSVKNVKGINLRKNEITNLSSFKVLVEFVNELELLDLRCNEIPQESVAALRPLKIQNLLISKNRFSLSYDVLCLFGKELPYVIKWTEKIFLRILPSKLNLKKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLN4</accession>
    <name>T1GLN4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLN4"/>
    <dbReference type="STRING" id="36166.T1GLN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004446-RA">
        <property type="protein sequence ID" value="MESCA004446-PA"/>
        <property type="gene ID" value="MESCA004446"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779560_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004446-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="14987" checksum="508CBAC353F509A6" modified="2013-10-16" version="1">MASASKVASSSSSSITSQNQSNIMVNSSNASSIAAAQAAAMSQAQEAMAHAAAAQANANAQLQAAVHQKQLEDINRAMIQRFARQQTGPPIGMVPVASAGHMNLVQPILRGPAMAITPQGAYIGSNMIRPGPPGMGGMPTGRFNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLS7</accession>
    <name>T1GLS7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004491-RA">
        <property type="protein sequence ID" value="MESCA004491-PA"/>
        <property type="gene ID" value="MESCA004491"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212931_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004491-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12042" checksum="C4A90837D67096AF" modified="2013-10-16" version="1">MSHFVIPKKLIKLIESYIETGNIIYNSVELSANGSKTKLIISSKNQQRHNDLGSNITMRLQNIVIVKDFRTSEVASDNNKPRLEDASSFETNNKAERSFVIVSGLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLW5</accession>
    <name>T1GLW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02098494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004530-RA">
        <property type="protein sequence ID" value="MESCA004530-PA"/>
        <property type="gene ID" value="MESCA004530"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1543882_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004530-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="19241" checksum="B790545C56C96F28" modified="2013-10-16" version="1">VAIITEPHFGVDSRIKKDNVPDISDAKKDEEEKELDELRELNKPKSDVSENKTNASSASKIEVLPESSKTYHGGARLAFGQRPEVLPTSTSATTKIVLTTPPLVGNIPEEMLNRGTEPSLSSTEDSSEEETEEETVISEQKESNPNEKEQLSQLELSSNKTEERRSESGLYKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMG3</accession>
    <name>T1GMG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">superoxide dismutase</fullName>
            <ecNumber evidence="1">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02099135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMG3"/>
    <dbReference type="STRING" id="36166.T1GMG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004737-RA">
        <property type="protein sequence ID" value="MESCA004737-PA"/>
        <property type="gene ID" value="MESCA004737"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056632_4_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577519" description="superoxide dismutase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="37"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004737-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="17102" checksum="A79954365C36252A" modified="2013-10-16" version="1">MLKIFVTLTVVAVGVFHLVQTHSVTQAISVLTSDYGVSGVIRFSQENCSAPLVMNISFSGMNPGWRGMHIHEKGDLSGGCETVLKHYNPLNNAHGAPWDAIRHIGDFGNVYVDQNGNVNKVICDHVAIHNGTDDLNRGNTELSSTTGNAGTMVGCGVIGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMH3</accession>
    <name>T1GMH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Structural maintenance of chromosomes protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC3 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02150384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMH3"/>
    <dbReference type="STRING" id="36166.T1GMH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004747-RA">
        <property type="protein sequence ID" value="MESCA004747-PA"/>
        <property type="gene ID" value="MESCA004747"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_281220_0_0_1"/>
    <dbReference type="OMA" id="RTAHRIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03272">
        <property type="entry name" value="ABC_SMC3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1620:FF:000002">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000424">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
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        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041741">
        <property type="entry name" value="SMC3_ABC_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="15">
        <location>
            <begin position="77"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CARD" evidence="14">
        <location>
            <begin position="551"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="441"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="610"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="712"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004747-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1115" mass="128288" checksum="1AD81CDD0F778F2E" modified="2013-10-16" version="1">MMVAELVARQHPLGSPINTDPVNYNLGHCNVSSICSPDHHPQYIIQTMDYGHQVTTTVSGNLPQRKQREFIPDNKKDDSYWDRRRRNNEAAKRSREKRRYNDMVLEQRVVEVTKENHILKAQLDAIKAKYNICGESLVSIDQIMATLPTAEQVLSITKRPKLSKITKPTFVGPTPVINYQSFQAQPPPSNIFSNQDVDVDVLNLSSRRSIDDEDEHNMVYNQDTQSNNNSLPLKLRHKSHLGEKDAAATALLSLHSIKQERNLLATSNSHSPLLWEKEMDGGSMSDVSDSGISEWGRHKNPVSEENCHLKNQIARLQSEVTAGNRLFHHIVDTDRVGTQILKEMNKQKLPGEVTFMPLNRLQVKIQDYPEDPDSIPMISKLTYEEEYDKALRYIFGKTLICRNLERATELAKTTGLDCVTLEGDQVSSKGSLTGGYFNTSRSHYEKELAALKNELKQIETSINQVVSDMQRTETKQGKSKDVFEKVHADMRLMKEEVQRIEKNRNPKERSAAQCKASLEAMNNTKSSLESELHQELRATLSSQDQKEVDQLNEDIRRLNQENKEAFTSRMQLEVIKNKLDNLLTNNLFRRRDELNVALQEISVEDRKRKLINCKNEVVSADRRIQKVNEDLKETDRKVQDAEKAQKTLQKELEMWTQKEKDAQEKINEDSKKMEKWAAKENILITKIDECSEKIAGLGALPQVDPTYQKMSLKNLFKELEKANQHLKKYDHVNKKALDQFLSFSEQKEKLYKRKDELVKAHEKIKELMQIADNFTKVFKKLCEQGAGYLILKTNNDSRYNESLENVAHSDAFTGIGIRVSFTGKDAEMREMNQLSGGQKSLVALTMIFAIQKCDPAPFYLFDEIDQALDSQHRKAVASMIHELSEKAQFITTTFRPELLENAHKFYGVRFRNKVSHIDCVTKEQAKDFIIIQGFKSYKDQTIVEPFDKRHNVVVGRNGSGKSNFFYAIQFVLSDEFTHLRPEQRQALLHEGTGARVISAYVEIIFDNSDNRVPIDKDEIYLRRVIGAKKDQYFLNKKVVPRTEVVNLLESAGFSNSNPYYIVKQGKINQMATAPDSYRLKLLREVAGTRVYDERKEESFPPPYRHFIYIIIYVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN00</accession>
    <name>T1GN00_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004939-RA">
        <property type="protein sequence ID" value="MESCA004939-PA"/>
        <property type="gene ID" value="MESCA004939"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707603_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004939-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8504" checksum="9C910E5343A9F09F" modified="2013-10-16" version="1">MGDGLLKNQKYDKILKKCADSAPKKRIQILLKPQICIFFCDVAADYVKMKVVLEIIFEKKIFIIIYPRKPHPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN94</accession>
    <name>T1GN94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sodium-dependent nutrient amino acid transporter 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02069649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN94"/>
    <dbReference type="STRING" id="36166.T1GN94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005038-RA">
        <property type="protein sequence ID" value="MESCA005038-PA"/>
        <property type="gene ID" value="MESCA005038"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_8_4_1"/>
    <dbReference type="OMA" id="NQFCNDI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005038-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="20406" checksum="3A094FBE3C0878A7" modified="2013-10-16" version="1">GFFSGLVYLTPGGQWILNLVDTYGGTYVVFCLAVFEMVGIFWVYGLQSFCDDMEFMINRKVTVYWRVCWTLITPGLMAIMFLYSIISLERIQYSGWEYPDSAIVAGWLIFVIGLIQFPLWTIWVITHNNNKTVLQLLKPTEEWGPVDSDLRANWKLFKRDREDERKRAHKTNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNI9</accession>
    <name>T1GNI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02102526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005143-RA">
        <property type="protein sequence ID" value="MESCA005143-PA"/>
        <property type="gene ID" value="MESCA005143"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173843_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12865" checksum="F60F73BC9075D38F" modified="2013-10-16" version="1">MSPNAEMALTILTITSFCNDKSRTAKWRSNQNDPSRRTACFQLLFCGIQQNFSKQSYHNFHRCFPSAASQNLEYRNDENDTQKRRENYLILSSTDDVDDYELDRSTSDCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNN9</accession>
    <name>T1GNN9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005197-RA">
        <property type="protein sequence ID" value="MESCA005197-PA"/>
        <property type="gene ID" value="MESCA005197"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929971_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005197-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="15126" checksum="B5A7612CCA89F673" modified="2013-10-16" version="1">MSRLAVSEISQTKLHLMIEFSDVVRNCKNFLRSCDYDEYLNNPYLVKEVSGKLPLSKMIKRQKKASCLESFAMFDSADYICIMDKILANDLGITGPVTFELIFNARNSTFQKRRPFSYTDSSLPRYSSRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP42</accession>
    <name>T1GP42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endocuticle structural glycoprotein SgAbd-2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GP42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005359-RA">
        <property type="protein sequence ID" value="MESCA005359-PA"/>
        <property type="gene ID" value="MESCA005359"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_678357_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF173">
        <property type="entry name" value="CUTICULAR PROTEIN 47EF, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="379"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005359-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="37952" checksum="36E073B3CC37F116" modified="2013-10-16" version="1">MFASLLAIGACARLDNTYLPPPNAQSAGGSGLPAPGGGYPGSGGGAGGGGGFGGGGGAGRPGAGGGYGGSGGGGRPGGGGGFGGGGGAPGGGGYGGGGGGAGAPGGGYGGGAGGGAGSGGGFGGGGNNGLGGFGGAGGPSRPIGPSPGRPQAPQQPQQPSGPPIEILSYENENYGDGTYKFSYETANGIKAQEQGEIKNKGSDNEIPSVMGSYSYTAPDGQVITVTYIADENGFQPQGDHLPTPPPIPEAIAKALAAAEAQRAQGGGGGGSGGYGGGQGGGGGYGGGQGGGGGGSGGYGGGSGAGGSGGYGGGSGAGGQGGYGGGQGGRGGAGSGGGFGGGGAGGAGGRGGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGRGFGGGAGASGGRGGGAGGPGQQYVPPKQPGSGSFNPQTGYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP69</accession>
    <name>T1GP69_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02128945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP69"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005386-RA">
        <property type="protein sequence ID" value="MESCA005386-PA"/>
        <property type="gene ID" value="MESCA005386"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2164699_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005386-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12636" checksum="EBD41F82C17633CD" modified="2013-10-16" version="1">CSIENTISFFVVEQTDFNSKHITSYIENLLSKGEKDVNPRDSVWSGFDGSHKKCSGSDYNIHEGTKASVIITYFNETQVNLFNTILSVVKKTNRKYLAEIIIIDDCSYNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPA2</accession>
    <name>T1GPA2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02190966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02190967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005425-RA">
        <property type="protein sequence ID" value="MESCA005425-PA"/>
        <property type="gene ID" value="MESCA005425"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834088_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005425-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7288" checksum="3A155054B17E5136" modified="2013-10-16" version="1">MGWEVLANIQCKSLESSESGVKSNLPNGHINFQLPQPGGNLGFSEIQSQQAHLWKFIPPIELTVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPS2</accession>
    <name>T1GPS2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02125071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPS2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005613-RA">
        <property type="protein sequence ID" value="MESCA005613-PA"/>
        <property type="gene ID" value="MESCA005613"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239682_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11297" checksum="FF952CD9C218953B" modified="2013-10-16" version="1">MDDDDCGSASTTANNNNTNHINNNLTLTPPSSPESVRTTGSCGSINDSQHHLQQQQQHQQHHQKQFYTKYSTTGATVTTSSTISAVNPTSIAQRGIATQNPQVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPZ6</accession>
    <name>T1GPZ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPZ6"/>
    <dbReference type="STRING" id="36166.T1GPZ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005692-RA">
        <property type="protein sequence ID" value="MESCA005692-PA"/>
        <property type="gene ID" value="MESCA005692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_446400_0_0_1"/>
    <dbReference type="OMA" id="ITEYEMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="3">
        <location>
            <begin position="482"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="612" mass="70574" checksum="BA183EEB0FDBF56B" modified="2013-10-16" version="1">MILDEILDDFEGILNSCSVDGVQKVVSCLCSSENKICENTLELIFKKVAEVIGRFGSDAPYDCVANVLKYCRKHYPENLLQYSKNLLASDMKNPFLAQEIYSLCFNDELIFNNAKEEALEFLMSGLTYGEFQSGNDKIEFDYVLEELKKDLTSPWKNYTCFPSQVRAVCLNALSSNIKSDDILKDLKELLIQKSIEISTKKPRYFKNSPHHRLKLRVICALAVLHRNENIRFWDSRLLKTVFDENNQTDVTLIAERIVAMTIPKDVLLELVSKLPTMTASTQVSLLSMCFMFCKINNDKALSQQFIQTFLPYTMGQNFITRLNAQITIYYLLTLFEITEYEMIKTAILDSFKTQTNQEVNLLKLNESIQHTVWSKDGCLDDECLFGVMYSLDTPVDEFYFGLAVLMYSFDPNGSKFCQRMITADDTIYDSRKKIYAPAPPPEEQKLDEFAITSNVQRKANPIQVSTDFKVFQEDYEEDEKEMIVVASLIDKIPNLGGIARTCEVLGIENLVISSLKFCEGTEFRNVSMTAEKYLNLSEMKPKFLQNYLIEKKTQGYSIVGAEQTSNSVNFISFKFPKKCVLVLGHEKEEFLQIYWDYWTMLWKYLRLELYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQP3</accession>
    <name>T1GQP3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005954-RA">
        <property type="protein sequence ID" value="MESCA005954-PA"/>
        <property type="gene ID" value="MESCA005954"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294852_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005954-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11530" checksum="3EE50798FB71C314" modified="2013-10-16" version="1">MSGCLGYVKGKEYTAIDPDHEIAIAMSDVRTLNRVGTKQDLKTCFKDTRYTSRHCKRLDGKETTIRMIQSDPGQLQTIDQCTCDESGKQLETLISHRSFKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR12</accession>
    <name>T1GR12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006083-RA">
        <property type="protein sequence ID" value="MESCA006083-PA"/>
        <property type="gene ID" value="MESCA006083"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880590_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09531">
        <property type="entry name" value="SAM_CS047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039161">
        <property type="entry name" value="C19orf47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040772">
        <property type="entry name" value="C19orf47_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21359">
        <property type="entry name" value="DUF5577 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21359:SF1">
        <property type="entry name" value="DUF5577 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18017">
        <property type="entry name" value="SAM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="14872" checksum="6C847347A42FBDB5" modified="2013-10-16" version="1">MSSGGAGQWVKFFNTAGIPSPTAATYAHVFVENRIQVDMLMDLNKEYLREMGITAMGDIIAILRHAKSVHDQSARDKVLSTSESKISSGYLETLHRPLTKSVVVAPSRRRSDSVEEVEDVHIVKAKKKESASGTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRU7</accession>
    <name>T1GRU7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein HIRA</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRU7"/>
    <dbReference type="STRING" id="36166.T1GRU7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006390-RA">
        <property type="protein sequence ID" value="MESCA006390-PA"/>
        <property type="gene ID" value="MESCA006390"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004372_0_0_1"/>
    <dbReference type="OMA" id="AWVHHDD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001071">
        <property type="entry name" value="Protein HIRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011494">
        <property type="entry name" value="HIRA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF0">
        <property type="entry name" value="PROTEIN HIRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07569">
        <property type="entry name" value="Hira"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="12">
        <location>
            <begin position="3"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="11">
        <location>
            <begin position="552"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006390-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="801" mass="89263" checksum="78F94C71B0D6483E" modified="2013-10-16" version="1">MILLKPEWVNHDDKPIFSVDIHPDCNKFVTGGLGGGDGSGLVRIWNIHPVLTVEAEADPSIPKLLCAMDTHLACVNSVRFSKNGMYLASGSDDKLVIIYHLAKGSSSGNFVCLHLRGHAGDVLDLAWSPTDRWLASCSVDNTIIVWDAQNFPTIVKVLKGHSGLVKGVAWDPVGKYIASQSDDRSAKIWKTSDWTCMHTVTDPFEECGGTTHILRLSWSPDGQYLVSAHSMNTGGPTAQIIEREGWKTEKDFVGHRKAVTCVRFHKDILKKQRDKSSKSQMYCALALGSRDHSLSVWMTCLKRPLFVVHQLFNDSVLDIAWGPNKYCLIACGGDGTVACFIFQEKDLGVPLSEEDKNAFYERSYGKSACVESGSSGRDMIVENAEILDAVKEKVQLPSLSGKPSQPEIVPNNHCEPSVNLFTQPAPPPVITSAEKCQLDNRLTKTTAPSAPIKSVSVLNSISSAIVEKPAKPVSGPAKLNVTTTSKDGMNSNTKWKYEYPLGNLSRIWGWCRNEKIWEVYSGAHVVNFTLCLKYALICCQDGTIRISDIKTGSNILPIMCMATSAIQCTFCPLGKLAGVISECGQIRIWNIDELKIYLTSSCSEIFSKSGQVVQFQITENGVPFILFSNGNAYSYSMDMQCWMQLYAKDAIFRQGFRSVIPKDFQRNMRQHPLMTVQSTTNNFLGNVSAGMDSNVNNWQDSAKLTFIENQIKLCEQINSPEELKYWYLMLGHHLAASGGEKKIRELLDYLLGSAHGFKKKTIKDDIMGVSKHYLLDSILDVLKKYPKWQRIYMEYFEQYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS55</accession>
    <name>T1GS55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS55"/>
    <dbReference type="STRING" id="36166.T1GS55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006503-RA">
        <property type="protein sequence ID" value="MESCA006503-PA"/>
        <property type="gene ID" value="MESCA006503"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_763514_0_0_1"/>
    <dbReference type="OMA" id="NTHIAKY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577659" description="SOCS box domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="4">
        <location>
            <begin position="271"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="363" mass="42519" checksum="A84CA1B215C12ADA" modified="2013-10-16" version="1">MTLNFFFKLFQVTQLVKAGSKIDAQNQSEQTALIISILNRNTHIAKYLISQNAKLNIQDNCGISALGYGVYKNNTELCELLVEKGARITRSREWLHAAIQNQNVEIARLLLRNNEDVEKASYIGTTTPFYEAITIGQPEMIKLLIEHGGGKKYRLSMQQLVIDCRDRHHFEMVIRIISRNHLSLKPVIMNDLILCCSYARFDFAEVLVREGFRIWEFNNIPELLTSARDYNLIRLFVSAGLKVQKLHLTTSVRSNQQSQTLEYLERIATNPRPLRDLSRLAIRKSILEVNLSNKFDLEKTIFSYLVQFLHLPLELKNYIELKEIPKLCHQNRCLLDNWMSFENQKNYQKLYWTRWTKNIYINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSE5</accession>
    <name>T1GSE5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSE5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006600-RA">
        <property type="protein sequence ID" value="MESCA006600-PA"/>
        <property type="gene ID" value="MESCA006600"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2801349_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577664" evidence="2">
        <location>
            <begin position="20"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7237" checksum="B0D18EE8FF3C572F" modified="2013-10-16" version="1">MSEISLIVLLNTLIAFVDTVPLKGTSLAVGATTIHKLNTPETEGLSPLISVLLLVFIVIVMLIICMCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSM5</accession>
    <name>T1GSM5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006685-RA">
        <property type="protein sequence ID" value="MESCA006685-PA"/>
        <property type="gene ID" value="MESCA006685"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608793_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006685-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8913" checksum="721F117885BA1E8C" modified="2013-10-16" version="1">MKTHRKEIVLYTSLIETLKSLGNILPEGLMTNRQLLQDLEQSDGDTTAVCMEGNNRKFAMVLSYFQHGGGIPTDCVRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSR9</accession>
    <name>T1GSR9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSR9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006730-RA">
        <property type="protein sequence ID" value="MESCA006730-PA"/>
        <property type="gene ID" value="MESCA006730"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707637_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006730-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8127" checksum="628779CC5FDDC7FF" modified="2013-10-16" version="1">MASSKLDSWTGAGVFIVWNCREELQTLSSQNHVKLSWVPGNSNIKGNVVSDNLARIGSMFKNKIKLKEMSIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTF5</accession>
    <name>T1GTF5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02143262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006985-RA">
        <property type="protein sequence ID" value="MESCA006985-PA"/>
        <property type="gene ID" value="MESCA006985"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838558_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006985-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="6940" checksum="6AE528AD9915B45D" modified="2013-10-16" version="1">MAPGDNVNIIADIGKKSYSESEVFQENFARQQAYFDALQRQAYYNSGAGSGSYAGAYAGGSGGGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTI2</accession>
    <name>T1GTI2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTI2"/>
    <dbReference type="STRING" id="36166.T1GTI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007014-RA">
        <property type="protein sequence ID" value="MESCA007014-PA"/>
        <property type="gene ID" value="MESCA007014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075838_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF28">
        <property type="entry name" value="P3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13296" checksum="DB5C212827EEF123" modified="2013-10-16" version="1">MINTIIVLAGMGLPWTGYVITYCVSKILRQTNKDALTIAIEAGIQNIGIAFFLLRFSLPQPYQDLTTLVPISISFMTPLPLILLVIIKKIFKLCEEEEVDKIIPVNLKEKEMETMLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTR2</accession>
    <name>T1GTR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02069404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTR2"/>
    <dbReference type="STRING" id="36166.T1GTR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007103-RA">
        <property type="protein sequence ID" value="MESCA007103-PA"/>
        <property type="gene ID" value="MESCA007103"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559149_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="126"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="18959" checksum="D331A1A130765D40" modified="2013-10-16" version="1">MKGNEMLLASMTEISEAEAPATVTTSYNTLPTSSQRTDRTFQYSLHVSNVVNISITTTSYEYIGGNIGNINGNNNNASAFGSNVTSIYNENTENIRINETDIARTLVEDNYWALLAIILVVVTAGGNILVCLAITWERRLQNVTNYFLMSLAITDLMVALMVMPFAILTLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTV9</accession>
    <name>T1GTV9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTV9"/>
    <dbReference type="STRING" id="36166.T1GTV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007153-RA">
        <property type="protein sequence ID" value="MESCA007153-PA"/>
        <property type="gene ID" value="MESCA007153"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1995217_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007153-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14238" checksum="15DA42C402E80BB0" modified="2013-10-16" version="1">MSLISPFNGVIREIMLEFRKELIKLITAISLFIISAKSHDCYRLESLVNSSDKEILVDVMQSPRKPRKDKTIFIHITNCMNNSEIYINPRQACAVESAALLNPSLDVFVLFAIQCLFPKPIVQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUG9</accession>
    <name>T1GUG9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02122848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02122849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02122850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUG9"/>
    <dbReference type="STRING" id="36166.T1GUG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007382-RA">
        <property type="protein sequence ID" value="MESCA007382-PA"/>
        <property type="gene ID" value="MESCA007382"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021727_4_0_1"/>
    <dbReference type="OMA" id="TICEINM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47111">
        <property type="entry name" value="BCDNA.LD29892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47111:SF1">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="213"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007382-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="395" mass="45068" checksum="A8BE2D184DFBB648" modified="2013-10-16" version="1">MASVSSSSSPGEIKSNIDIYDIHEDLVKTIASMNKLHVSSSAFFRLTENYQRVKKEGNENFSLKSRNYFRALELYNKSICYSLSDSENISIGYANRSAVLFELKNYQQCLENISMARKANFPQRLMHKLDKRENDCKKLLTQAQATDLKSHEFVLSHESHQTVPFISNCLEMGCSKEEGRFIYTKTALNVGDVVAMEDPYCTALLPAMRYIRCAYCTKEACFTLIPCGNCTSAMFCSEDCREKGWREFHKYECPIIDFLLDSCTKIHLTALRTCFVTLSLFSNINECRAFCENSDNIDKNVFDLNYKEISKLEEYRAVHGLVTNEEKRPVNDLFQRATLGATIKNILLEFTPLLNHSCAPNVVRIHKGTTAILFVLRNIEANGKLYDNYGNTNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUJ1</accession>
    <name>T1GUJ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUJ1"/>
    <dbReference type="STRING" id="36166.T1GUJ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007405-RA">
        <property type="protein sequence ID" value="MESCA007405-PA"/>
        <property type="gene ID" value="MESCA007405"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046907_0_0_1"/>
    <dbReference type="OMA" id="ADWGILF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF142">
        <property type="entry name" value="POTASSIUM CHANNEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="9">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007405-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="235" mass="26136" checksum="09DB2741C1390BD3" modified="2013-10-16" version="1">YGNIVPVTIGGRAFCICFAIIGIPFTLTVIADWGILFATAVSVLGKHIPKKPSIKNVIGKTWFYALAAVCFLCLYLAIGAGLLLLWEDDWTFFDGFYFCFITMTTIGFGDLVPTEKPNYMLLCTLYILIGLALTSTIIELVRRQYAQSWQKLQELSGPMADTLRRLQLQAGSGLDYASLQKVLTVSMPKWNTSSGNKKKDMAALEAITNAILKDINENQQQKQKIVQIIIYESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV71</accession>
    <name>T1GV71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007655-RA">
        <property type="protein sequence ID" value="MESCA007655-PA"/>
        <property type="gene ID" value="MESCA007655"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433185_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007655-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10385" checksum="5AAC0EC04904CD29" modified="2013-10-16" version="1">MFKLRIVNRNESNVNIQLLTSDSDSSRNGNEDCCKDNNIHFDDKNNRKKKMALEGKAVIKEEITEKDTYDSTNSTSNNNNNNNTTSGRRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW11</accession>
    <name>T1GW11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007979-RA">
        <property type="protein sequence ID" value="MESCA007979-PA"/>
        <property type="gene ID" value="MESCA007979"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075841_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577782" evidence="1">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007979-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13401" checksum="D3F7DD1F3693D5C2" modified="2013-10-16" version="1">MKVILVITLLFGLTLAVDMEEDTWVAMKNKELLHLSCAQELHIENKTEDTIGYKEFKCLATCLMEHRGYLKHGEVDVHEVAKAIKEKTHLEKHLKSKLIADLPTCAKLSSTRSTVIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW33</accession>
    <name>T1GW33_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW33"/>
    <dbReference type="STRING" id="36166.T1GW33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008004-RA">
        <property type="protein sequence ID" value="MESCA008004-PA"/>
        <property type="gene ID" value="MESCA008004"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_988922_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF151">
        <property type="entry name" value="PHOSPHOLIPASE A1 MEMBER A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008004-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="32086" checksum="FED8D92AFA6FB7F5" modified="2013-10-16" version="1">MVTPHCRKLSCLDPAMPLYITAGVTDKLDPSDAEFVDVIHTNALVQGKLEQCGHVDFYMNGGIMQPGCLCPPTNFLVEAGENTRPTTKGMFLIQTNEQFPFAKGKWTDISAEISNTQPSLTQPLRRTQGPLLKAVDEWGKLEGNFNNKDHFSTPYSQDPYGTNWPYFNDNGPQEITQDLKSDEEDFTYPQRRPINSKFKSKLRKRPLNRNGNNRPVYRSTRIQMLTQMLANMRATAIGKENGQDEDRSASQCDMYYRCDPTNHEFGVGITVRGKTRFCVTRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW90</accession>
    <name>T1GW90_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02156954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW90"/>
    <dbReference type="STRING" id="36166.T1GW90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008062-RA">
        <property type="protein sequence ID" value="MESCA008062-PA"/>
        <property type="gene ID" value="MESCA008062"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1580303_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070920">
        <property type="term" value="P:regulation of regulatory ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19862">
        <property type="entry name" value="DSRM_PRKRA-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000120">
        <property type="entry name" value="RISC-loading complex subunit TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051247">
        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF3">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="76"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008062-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="17755" checksum="F822C9A5575E4135" modified="2013-10-16" version="1">MPENTDSAAAGADPSVPPNGCGYAPRKMRGPAKNSAAQKIDKNSKSENKENSSAAQVLDTSLASSGNSSLSSAIKTPVSILQELLSRRGITPNYELVQIEGAIHEPTFRYRVSFNDKEISLTAMGSGRSKKEAKHAAAKALIDKLTGVSVVERTTVSNDSKSGVSSGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU4</accession>
    <name>T1GWU4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189745">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008275-RA">
        <property type="protein sequence ID" value="MESCA008275-PA"/>
        <property type="gene ID" value="MESCA008275"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1679959_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008275-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="17394" checksum="845B69894601F128" modified="2013-10-16" version="1">MDTKSIISSLLVRAQHSHPYSFTSWIVGNILKNATTAASQQLLPQTSILLASTIPTTTSITSSATTTFASVLNAATLKESNQITSFVPSESGNVDEFLWYVKIERATDHCFPPLEKGKENTDRGKNEKRKSIFHSPQNEADGSLWKVFFLSLKIWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY02</accession>
    <name>T1GY02_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY02"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008717-RA">
        <property type="protein sequence ID" value="MESCA008717-PA"/>
        <property type="gene ID" value="MESCA008717"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147604_0_0_1"/>
    <dbReference type="OMA" id="NNAYEMK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008717-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13978" checksum="CD58D1F79A4A587C" modified="2013-10-16" version="1">QYQLLRQLNSVSEGQAILPSHISDRVQVLQEFLKSSLEKSHKEYEKRYNLRSRPVKFNQGQTVFKRNVILSDKNKNLNAKLCPKFVKCLVRNVLSQNRYELEDENGKFLGIYHAQDIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYN1</accession>
    <name>T1GYN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Glutathione synthetase</fullName>
            <shortName evidence="13">GSH-S</shortName>
            <ecNumber evidence="4 13">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13558"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="4 13"/>
    <dbReference type="EMBL" id="CAQQ02043262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYN1"/>
    <dbReference type="STRING" id="36166.T1GYN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008966-RA">
        <property type="protein sequence ID" value="MESCA008966-PA"/>
        <property type="gene ID" value="MESCA008966"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025152_2_1_1"/>
    <dbReference type="OMA" id="FEAHKNM"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1760:FF:000001">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="16">
        <location>
            <begin position="166"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="329"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008966-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="416" mass="46685" checksum="45B0E5939DF6A948" modified="2013-10-16" version="1">TNPTNDTLEFAPFLLTPSVFPRKQFEKAVNLQTTLNELVHAVAYDDDFLTGTLQMTIQADDFTKRLFDIYKTVKSENLLNTVNLGVLRSDYMLHSDNTIKQVEINTVASSFGAVSSVIAKTQKYIMGELGFGGADHEKNLPVNSSVQSICRGMIRAWELYNNENSVIVFIVENLTMNICDQRILEFHIRETNPKIQVLRLTLTEMASAATLDESFKLKLRGKYGDVEVALVYFRAGYEPNHYPTEDEWNARLMIERSRSLKCPSINYHLAGTKKVQQALAVPGILKRFINDEDKIRMVEDVFTGLYPLESLSAYEMAMNEPGKSAWILMDRIVPPTIQGYVIRSGTKDLIVNDCVSELGIFGIIIDNADNIFENYQAGHMLRTKLSTVNEGGVAAGLGAVDTPYLIDDDQVTLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYW5</accession>
    <name>T1GYW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYW5"/>
    <dbReference type="STRING" id="36166.T1GYW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009052-RA">
        <property type="protein sequence ID" value="MESCA009052-PA"/>
        <property type="gene ID" value="MESCA009052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1733579_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="16732" checksum="76273BDD8592713A" modified="2013-10-16" version="1">MKNHMKTTHEMYTLSTSASKDSSPVVQSDLNGSDSDDQMDTSLMEPQLMLDEYDEPVEFKYDPNTDQQDNGNYIQQQSQYKLMMAAAQQRQLEQLQQLQAVAKGGAIIGVGPQPGTVTISNASNSLVSIPKLTPILSKTLKQCQSYQNVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZS1</accession>
    <name>T1GZS1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009376-RA">
        <property type="protein sequence ID" value="MESCA009376-PA"/>
        <property type="gene ID" value="MESCA009376"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3089604_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="5996" checksum="8473A64A18BC955E" modified="2013-10-16" version="1">MKPHLYDPMLQMKVSFNLSHGCHDLIGNVKDTYSSVVGTIYLVPFLWRSIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H051</accession>
    <name>T1H051_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H051"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009519-RA">
        <property type="protein sequence ID" value="MESCA009519-PA"/>
        <property type="gene ID" value="MESCA009519"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3002470_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009519-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="57" mass="6484" checksum="79DBA1C149A1313E" modified="2013-10-16" version="1">MTSVGIMGAALLDASEEANMDNYVSNDEIGNHFKVMVYGNERYIRAMDNILPWMDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0H3</accession>
    <name>T1H0H3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0H3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009651-RA">
        <property type="protein sequence ID" value="MESCA009651-PA"/>
        <property type="gene ID" value="MESCA009651"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1457930_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="110"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009651-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="21328" checksum="85CA3F070B0A3160" modified="2013-10-16" version="1">MTRERLEIRREFGLPVGTKLQAPKFSRKQSVGRGMDLDPETQARYQAALDRLSQAPCESKKTRYEQIMDQERIASKISFKPREKVTAPAPVCEPEIFPTVCEPEVSAPKVVELNKMYETYQKDVQKIDEALQTAHLDEEEINELEEQKEFLALELELHKKAVCEQPAMASVCDQRPIFEAESMPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0J3</accession>
    <name>T1H0J3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0J3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009672-RA">
        <property type="protein sequence ID" value="MESCA009672-PA"/>
        <property type="gene ID" value="MESCA009672"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075824_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009672-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13621" checksum="05B7261BCF533D49" modified="2013-10-16" version="1">MSVYCSLIDVCIERLILLVQAWKAGRLARGVHKKTTLTSSSLSSLSELERILDKYCKTWLFLRNNIKTLVDRHERVTQCLALPLTIKPTSNSLFSGVQLKYSIALRFRNMPHWGKDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0K6</accession>
    <name>T1H0K6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr5a subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02064432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0K6"/>
    <dbReference type="STRING" id="36166.T1H0K6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009686-RA">
        <property type="protein sequence ID" value="MESCA009686-PA"/>
        <property type="gene ID" value="MESCA009686"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043581_1_0_1"/>
    <dbReference type="OMA" id="CHEIRAD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033041">
        <property type="term" value="F:sweet taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009318">
        <property type="entry name" value="Gustatory_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421:SF29">
        <property type="entry name" value="GUSTATORY RECEPTOR 5A FOR TREHALOSE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06151">
        <property type="entry name" value="Trehalose_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038981">
        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009686-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="40507" checksum="28B4B50897300D32" modified="2013-10-16" version="1">MPVKGIKCKNAENLSFSWKNFRTISCVLFIISTIIDTGFTCYMVFDQPFQFSKIEPVVFHIVLFLVILNLLKLSRKWNTMIKKWLRVEEILPQYEKAAERTVLSRRINSIAIVFFMSALVEHLLSVISAIHFANYCPARKDPIESYLFSSSNQLFYVFDYNTIIAWLGKMENVLLTFTWNFTDVFLMIIGVALSSKFQQVNDHLEKHKHEHKPQEFWEQSRIQYRRVCNLVEEMDDEVSMIVLLSFTNNLYFICMQFLKSINEMPSMAHAIYFYFSLGFLVGRTLAVSLYLADINDKSKITLSIIRHVPEKTWCPELIRFCHEIRADLMALSGMKFFNVTRKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0S2</accession>
    <name>T1H0S2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0S2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009759-RA">
        <property type="protein sequence ID" value="MESCA009759-PA"/>
        <property type="gene ID" value="MESCA009759"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504273_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009759-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="10231" checksum="AFDAAD175F9FC14E" modified="2013-10-16" version="1">FREKIPSDNLRTDLRKRGVENKVQPRAEAIVSKTKTLSKRSELKRQVILFESRKTCLLEMYSPQIQLEIEDLVVRKIDGLSWWRTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1V5</accession>
    <name>T1H1V5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Peroxin/Ferlin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TECPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02195820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H1V5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010175-RA">
        <property type="protein sequence ID" value="MESCA010175-PA"/>
        <property type="gene ID" value="MESCA010175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397561_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051513">
        <property type="entry name" value="Tectonin_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250:SF1">
        <property type="entry name" value="TECTONIN BETA-PROPELLER REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06462">
        <property type="entry name" value="Hyd_WA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00706">
        <property type="entry name" value="TECPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Peroxin/Ferlin" evidence="4">
        <location>
            <begin position="89"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="5">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="402"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="693" mass="77615" checksum="48675CB4732F7E8C" modified="2013-10-16" version="1">MILTKINLKKTVHPQKYVFSLKISRINAKFSSICTNNEGRVYGLSTSSSAWKESYTGMEFKKISSIPSFLWAIGGDRQVYVHVHGMDVPIRVKEESYENERWYPVEGFSKALLPTDRPKFSNIDGTVDRTIDKIRLPSMAWQWEGDWHLDQTFNGQTLEHDAWTYALDFPATFQAKKSWNTCVRRRKWIRFRRYSALNSWCAVAPLHKDPTQEPFMDISVGGTNIPGAKPGTFVVWSITALGRVMFRSGVSTLCPEGIRWIAINTPSNTEVSKIAVGTSGLVWAILHNGRALVRTGITRENLTGDSWLEVKSPSPGLKLIDVSIGSNSVWNKEDMAIGNGWVEMVGNISIISVAQNDQVFAIGSEDRAIYYRSGVSDSDPTGKKWRLIQLPMQFSRTSSTMSLISRKSGSSSPGSGSKRKSLSSLFKEKAVVESPAIIENVQSDSHLSSPRNKDVWKNSYELQSPHLPNVGSLNDHRRKPNYRLSDLQQAYSAPAADIQEIHGKHFETQLKHPGAWSPVRSAGSVVGTEAHAESDSAIFEADNSYRNSGCFGDDDDNIGSQYWAECDVIWTGCSAGAVFIDPKNLPNWFNSLINEDTQTDISSSWRIKNLENFKQRDNLYKTLDLSKYEKAIEMSSWVKSGEARVLKPGGNWEECLIEIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H235</accession>
    <name>T1H235_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H235"/>
    <dbReference type="STRING" id="36166.T1H235"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010266-RA">
        <property type="protein sequence ID" value="MESCA010266-PA"/>
        <property type="gene ID" value="MESCA010266"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239685_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010266-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12137" checksum="768042B6C0470200" modified="2013-10-16" version="1">MQHAPISNIPNGDVAKGETIASYVQPFPAKGLGYQRVVFTLYKQNAKVDFSSFKLADVQYIPIRFERFLGVQLECYKLRNTKLHTTPFLWFQLCRIPDAIQSWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2P8</accession>
    <name>T1H2P8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2P8"/>
    <dbReference type="STRING" id="36166.T1H2P8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010501-RA">
        <property type="protein sequence ID" value="MESCA010501-PA"/>
        <property type="gene ID" value="MESCA010501"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764131_0_0_1"/>
    <dbReference type="OMA" id="REYHYEM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF143">
        <property type="entry name" value="SERINE PROTEASE GD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="64"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010501-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40762" checksum="ADAD52C3A8D079DC" modified="2013-10-16" version="1">MRIDCLTSNIIAGTKANIDCQNGYTSSVNHTKILKCQENGKWDQRIRYCQPICGKIPDQIQGFVVGGKVTSIKNIPWQAIIYKKIDGNYDHICGGSIISELLIVTAAHCLLDDNRNFVPESSLRVGVGKTSREYHYEMGSTKPQIRDIRKYQINMKFSKDSFFYDIATNVIGKVAGWGLNEGEYFGSELAYADFKSISKNDCQYEVGTISSFVRKDKFCVKKDEFGMTLCKGDSGGGFAVKVDGTFFLEGVVSAGLLPNNESCNTHNFYTTLSNIQGNARTFVIRWKPNMVCWGRPSKSISYRCAGSVISENFVLTAAHCVTNLVDDLKVYLVRLGDLRSSRKLNCKDDPIRCKYHQDFDIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H390</accession>
    <name>T1H390_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">39S ribosomal protein L9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373546">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H390"/>
    <dbReference type="STRING" id="36166.T1H390"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010709-RA">
        <property type="protein sequence ID" value="MESCA010709-PA"/>
        <property type="gene ID" value="MESCA010709"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111625_0_0_1"/>
    <dbReference type="OMA" id="CVTITIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010709-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13473" checksum="299F2D4B290B0C78" modified="2013-10-16" version="1">MLESLVVPVSMNKDNPWVIEPWHIKASLRKMGYHCKDECITMPERKIEGPDLLKEGKEFCVTITINNLEKANVRCRIHHWSTEPSERLPYVFEYWNRPAENVFGSESEPVQSETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H430</accession>
    <name>T1H430_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H430"/>
    <dbReference type="STRING" id="36166.T1H430"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011029-RA">
        <property type="protein sequence ID" value="MESCA011029-PA"/>
        <property type="gene ID" value="MESCA011029"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_774550_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF40">
        <property type="entry name" value="ALLATOSTATIN C RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577863" description="G-protein coupled receptors family 1 profile domain-containing protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="209"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="299"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011029-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="358" mass="40708" checksum="BB6CBD4780264B63" modified="2013-10-16" version="1">MTNFVILSIYILNIICSTGCSNQVAQHKYKEIPEEIDSKTISYLENNYLSTEFIDNTSSTDKINYHNYSSYSTKSSKSDEFNLNSTDKTQPHCIPGSNALGNLIPIILYSVVCIIGLFGNTLVIYVVLRFSKMQTVTNMYILNLAIADECFLIGIPFLLTTMHLRVWKFGEYMCKAYTKITHCWTKEQIQRKEEITSKSHQIGTHCHKVYILCWLPYWISQVALINSPLTECASSLEITMFLLVGCLGYSNSAMNPILYAFLSDNFKKSFLKACTCNNGIGFIFTNFRRSRNNDHYFNNKTSKHNQQHSKLKKQPLQSSTDMLDGDEEGEQTAMTFQSKSVIRNHPESVVAIDEGPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4F2</accession>
    <name>T1H4F2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Splicing factor SF3a60 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4F2"/>
    <dbReference type="STRING" id="36166.T1H4F2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011161-RA">
        <property type="protein sequence ID" value="MESCA011161-PA"/>
        <property type="gene ID" value="MESCA011161"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592521_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021966">
        <property type="entry name" value="SF3a60_bindingd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12108">
        <property type="entry name" value="SF3a60_bindingd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor SF3a60 binding" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011161-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9295" checksum="0A2D857DC99DFDE3" modified="2013-10-16" version="1">MSSRINKLDNLCEDKDDKRKTVQALSCTNEFTEFYNRVKQIKEFYPDEISIPLSIGSEETTRVYLNTDSLVEFTDEEGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4M4</accession>
    <name>T1H4M4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4M4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011237-RA">
        <property type="protein sequence ID" value="MESCA011237-PA"/>
        <property type="gene ID" value="MESCA011237"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239658_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009635">
        <property type="entry name" value="NPDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23352:SF2">
        <property type="entry name" value="NEURAL PROLIFERATION DIFFERENTIATION AND CONTROL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23352">
        <property type="entry name" value="NEURAL PROLIFERATION DIFFERENTIATION AND CONTROL PROTEIN-1 NPDC-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06809">
        <property type="entry name" value="NPDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011237-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11978" checksum="D0B9513C870B5AF4" modified="2013-10-16" version="1">MRICYITIIRPCREISQTSGDKRLAQSAEIYHYQHQKQQIIAMEKNVPNDLNNAAILMLKAKMTMKKGTIPSTNAPGWHQGKPLDFKVKISTLISKNIQILDEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H593</accession>
    <name>T1H593_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H593"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011468-RA">
        <property type="protein sequence ID" value="MESCA011468-PA"/>
        <property type="gene ID" value="MESCA011468"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485925_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011468-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10201" checksum="C52CBD715D65BF75" modified="2013-10-16" version="1">MFYSAFISTTSRCAFDNYENMMRLRESIQGKARTYVESLLIHEESVSQVVSNLKIIYGRDDQIIFFLIKKVKFKKIVLLDFIPLSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H686</accession>
    <name>T1H686_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H686"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012219-RA">
        <property type="protein sequence ID" value="MESCA012219-PA"/>
        <property type="gene ID" value="MESCA012219"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_910812_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012219-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="306" mass="33966" checksum="CEF81A621859BB35" modified="2013-10-16" version="1">DKIFGLNIKKRTKSLKFNQQVHKVHAGGQHPRNTRHEERXXXXXXXXXXXDEKDGGMESWDDAKLKDVVNQKHGKEKNRPTTDIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNPDLVDDGPVEDGDFAFDNYPRSDDEDENTVEFKELDLNELSLAAQEVAGTGTMASSLQEQNGEPKSEEAAGVSGDNAAPINENLFLGLEDELNGLDVEDEDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6B3</accession>
    <name>T1H6B3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spondin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6B3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012246-RA">
        <property type="protein sequence ID" value="MESCA012246-PA"/>
        <property type="gene ID" value="MESCA012246"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1492691_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2130">
        <property type="entry name" value="F-spondin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009465">
        <property type="entry name" value="Spondin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038678">
        <property type="entry name" value="Spondin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06468">
        <property type="entry name" value="Spond_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51020">
        <property type="entry name" value="SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spondin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20465" checksum="2B081355589C5025" modified="2013-10-16" version="1">YDDFTLPAITTGSGRSESKFFVDGNHSHVSLMTKILPSPDWFIGVDSFQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYETVKTQTRLESEHNHVEMNNELSASIEKERQNEVTEGPVLTTEKDRIRARLLEKINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6C9</accession>
    <name>T1H6C9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6C9"/>
    <dbReference type="STRING" id="36166.T1H6C9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012262-RA">
        <property type="protein sequence ID" value="MESCA012262-PA"/>
        <property type="gene ID" value="MESCA012262"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3162650_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012262-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="48" mass="5590" checksum="46AAEF8C5F4352C4" modified="2013-10-16" version="1">IGTGHADTSKFEWLTNQHRDSLASYLGHYDMLNFFAITENESKARIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6G2</accession>
    <name>T1H6G2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H6G2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012295-RA">
        <property type="protein sequence ID" value="MESCA012295-PA"/>
        <property type="gene ID" value="MESCA012295"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051922_1_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012295-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="26700" checksum="A35A3055832143C2" modified="2013-10-16" version="1">IQYLFCTGKSGNKDVCAEDKFKEDGDPWSVEAQAAFLGPILWDKTLPYDADLKYEDLDEFLSENNMPDGLTGGHIANSVGFSHRSDSLGHSAALSLGLGHVTTKKERSPSPSDCISPDTLNPPSPAESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNATLHQEVEQLKQENMDLRSRLSKYQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6K9</accession>
    <name>T1H6K9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6K9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012342-RA">
        <property type="protein sequence ID" value="MESCA012342-PA"/>
        <property type="gene ID" value="MESCA012342"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1173175_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="23"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012342-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="237" mass="26089" checksum="633B54BDC4A1C483" modified="2013-10-16" version="1">MGPNGTATQQQPQQPGGEQLSKTNLYIRGLQQGTTDKDLVTMCTLXXXXXXXXXXXXXXXXXXXXQMAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGFARMESREKCEQIIQLYNGYEMPGAKDPLLVKFADGGPKKKNPFKSPDNTARTWREVTTEGIPVTYDPTISQNGVSVTPLGVPYTRFATPPATGHPKGSQTWIPYTMMPQGIAQADEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H707</accession>
    <name>T1H707_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H707"/>
    <dbReference type="STRING" id="36166.T1H707"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012489-RA">
        <property type="protein sequence ID" value="MESCA012489-PA"/>
        <property type="gene ID" value="MESCA012489"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2678098_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022209">
        <property type="entry name" value="CWC25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12542">
        <property type="entry name" value="CWC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012489-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="9025" checksum="E49D18A31D454A0C" modified="2013-10-16" version="1">MYKNSSELLNRDEYLLGKSVDKNFEELIDEKLQQIGIKSQKNHVEHDVVPFSIREYKQRKLMEDPLMAIRQKEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H739</accession>
    <name>T1H739_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H739"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012521-RA">
        <property type="protein sequence ID" value="MESCA012521-PA"/>
        <property type="gene ID" value="MESCA012521"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009082_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047242">
        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021786">
        <property type="entry name" value="Cdc5p/Cef1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11831">
        <property type="entry name" value="Myb_Cef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="370"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="118"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012521-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="585" mass="66733" checksum="C6EFD17CE7E407EB" modified="2025-10-08" version="2">MRIASLLHRKSSNQCKARWHEWLDPIIKKTECLREEEEKLHLPKLMPTQWRTIAPFIGRTADQCLERYEYILXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIGCGNRKRLKGIDYNAEIPFEKRPALGFYDTSEEAVEKLEPDFNKMRQQDLDGELRQEKEEKERKKDKQKLKNRKENDMPMAMLQNQEPARKRSKLVLPEPQISDQELQQVVKLGRASEMAKEVAAESGIETTDSLLADYTITPQVAVTPRTPAPYTDRIMQEAQNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPGDGSGNTPGSFATPVSTALVPVGGKTSRTTGLVRDKLNINPDDGMGVTETPALYKNYQNQVKQSLKQGLSSLPAPKNDYEIVVPEQEEGASETVEDEEMIEDQADVDAKRLADLELARKKELEKRSQTIQRFLPRPTEVNTKILRPPTEKQNLTDLQKAEEQIKHEMITMMLYDSVKDPIPGQSQNKMDQLQNYFKQNPYEEFSKDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7M0</accession>
    <name>T1H7M0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H7M0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012757-RA">
        <property type="protein sequence ID" value="MESCA012757-PA"/>
        <property type="gene ID" value="MESCA012757"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_895951_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.540">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027378">
        <property type="entry name" value="Nucleotide_channel_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF79">
        <property type="entry name" value="NA[+]-DEPENDENT INORGANIC PHOSPHATE COTRANSPORTER, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012757-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34999" checksum="9145D3E5DBA9C7A3" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIPYDKDGFDWNDKVQGLVLGSFFWAHWITQIPGGILARKYGTKLIFGLTNAIGCWLCIIMPIASYFDYRILMFLRVLQGCIVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGLISGLPHVMRMSFAMVFSMFGDYLLRTEKMSTTNVRKLATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA90</accession>
    <name>T1GA90_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ataxin-2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003499">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA90"/>
    <dbReference type="STRING" id="36166.T1GA90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000140-RA">
        <property type="protein sequence ID" value="MESCA000140-PA"/>
        <property type="gene ID" value="MESCA000140"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_294792_0_0_1"/>
    <dbReference type="OMA" id="MDNINEP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
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    </feature>
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            <begin position="424"/>
            <end position="490"/>
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            <begin position="519"/>
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            <begin position="993"/>
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        </location>
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            <begin position="10"/>
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    </feature>
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            <begin position="54"/>
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            <begin position="188"/>
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    </feature>
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        </location>
    </feature>
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        </location>
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    </feature>
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        <location>
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        <location>
            <begin position="1000"/>
            <end position="1015"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="EnsemblMetazoa" id="MESCA000140-PA"/>
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            <dbReference type="Proteomes" id="UP000015102"/>
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    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="1028" mass="116818" checksum="2999E9E5A1F48D9A" modified="2013-10-16" version="1">MVNNEILSQETEENTEEPEISTFVEEITSKKLPRETVEISESKTTTFVEQQVPEVKEEIKDEEPISEEPKKVITEKVPEEILEVTEPISEEPKEVITEKVPEEIVEVSEVKSVEIKQSEITPEQDPEKVEEPVSEEQVREEIIEVVEVSESITTEIVPAESTYTSTTEQQTTVTTTVVHSDNWLSEQSTQWMSTTSEQPKWSKGEDERPVIDTWSSVLKENPTDNIIASPDFGTITESTSYISSLSADAPEFKPSLSADAPEFVPSVKPQYDIDAPEFIPSSRRYPEQDPNTIFITQEQVYSSARQQHHKKHHKQPKTHKPRADPLTELVDEAIKQHTEAQKNIKPKKEKKVPTVVKEEKQTVEKVEKIEKKVIEPAPEAEPVTQKEEILETKQPTESTTSFSWASLVSKPGSWVDTTHVEEVTVTESAEEKVKKPIKEQTPKTPKAKPVKEKKAKQPKKKEPQKKLKWKLKKNPNPIPTIKNVEVDQKPTGPTWASLIKEPANWVDTTLVTEVHVVKEEIPKQKPKTPKPKIVKEKKVKQQKKPKVVEESLTESDVAVEKSESDSEPIKTPSKEIDDSQDSGPTWASLIKQPTEWVDTIKERKQIIEVKPVEEKPKHDRAPKPKAKKEKVHKKKVVPQEFVSTTEEEVQTTSSDSDQLEESTSAEEHLTIENAAEEKAEGGFTWASLIKNPTEWVDDIREKQQSKKEEVEVVEKPKPTPRKQKQPKLRKRPSIDDITNISKPKKQKRQKKEKKEEIELEPEPEITEPVEFHNEYVVNVTEEKSDPNEVEINKTFSNDSVPSWASIVQSSTTTTVVSTTTNTKPKETKPKDTVEPIKEHKVPSTPKPPTQKHSWATLIDAEIKGEDIDWWSESKVTVSESEDKKLEEEVLKAETIEDKPMSVITKTITTVTTEEKLQESKPNEQEQPKAETSEKNFVTTEVKPEEPKPKEPEVIVKEQPKTEEPKKAAYAGLPVDESTDAWMDNINEPMVFSDDEEESEAVTTTVTTTTVTTTTTTEEEPKVKKKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAD4</accession>
    <name>T1GAD4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S-adenosylmethionine synthetase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1GAD4"/>
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        <property type="protein sequence ID" value="MESCA000189-PA"/>
        <property type="gene ID" value="MESCA000189"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1706291_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
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    </dbReference>
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    <dbReference type="Pfam" id="PF00438">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="7"/>
            <end position="77"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000189-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17019" checksum="CDF2188BD8508BA3" modified="2013-10-16" version="1">MEDGKSFLFTSESVSEGHPDKMCDQISDAILDAHLQQDQNAKVACETVTKTGMILLCGEISSKAVVDYQKVIRNTVQFLVMINLLRIAAGVLIARSEEEISACEQGIMFVYATDETEEPQLLYSHTMMLPAMAAVVHVTPWMAKAKYLPIPVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAP3</accession>
    <name>T1GAP3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
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        <dbReference type="NCBI Taxonomy" id="36166"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000302-RA">
        <property type="protein sequence ID" value="MESCA000302-PA"/>
        <property type="gene ID" value="MESCA000302"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536305_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000302-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="19483" checksum="B242B345DF6A7382" modified="2013-10-16" version="1">GQNLTPQSLLHFYDVPSARLVGWKFGPHASGNSPHLSLEAKFYSIRGILVLELGQLVERIGFGQLRGGQLELEHGILGLELGQLVEQIGFEQLLGGQLELEHGILGLGLGHDILELEQKQLEPSIVCSHWWIQSFVLDIVGSLEFLHGGSFEQLELRIVECKKRHFRGMVASRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAX4</accession>
    <name>T1GAX4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02182836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAX4"/>
    <dbReference type="STRING" id="36166.T1GAX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000390-RA">
        <property type="protein sequence ID" value="MESCA000390-PA"/>
        <property type="gene ID" value="MESCA000390"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018190_1_0_1"/>
    <dbReference type="OMA" id="KINIYQR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF8">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000390-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="36581" checksum="0120A53218D6426C" modified="2013-10-16" version="1">MDLPFVSSSVLLLETVRLMMKMHAFVRTNVGWQLQGKLKSDSKAQVPLSKFSSYLYFLFAPTLIYRNEYPRTKQIRWKFALSCLSEVIAIMFVYSYVHERYIDPLFKKYGEEEITRASLSVKIFGMLMPSTIIFLCGFYLILHSWMNFAAEVLRFADRMFYKDWWTASNYEAYYRNWNIVVHDWLYEYIYKDMYNHIFKGSKLMSSLTVFFVSAYVHEHILGFALRMFYPILYIFFGGFGVLFIAMTKNLPKHFGNFVVWFTLITGNSLMIALYVMEYYARQNCPVGDSLIDYIKPVSWTCHGITDVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAZ5</accession>
    <name>T1GAZ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Immunoglobulin I-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02088770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02088771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAZ5"/>
    <dbReference type="STRING" id="36166.T1GAZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000414-RA">
        <property type="protein sequence ID" value="MESCA000414-PA"/>
        <property type="gene ID" value="MESCA000414"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944337_0_0_1"/>
    <dbReference type="OMA" id="ECLFENR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin I-set" evidence="1">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000414-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6667" checksum="98C06F8A111FBB4D" modified="2013-10-16" version="1">MDISLHHRFAIGQYVDVSGDVISHLNISHTRADDGGLYQCTATNTMGSVTHSSRLNFKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB34</accession>
    <name>T1GB34_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB34"/>
    <dbReference type="STRING" id="36166.T1GB34"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000455-RA">
        <property type="protein sequence ID" value="MESCA000455-PA"/>
        <property type="gene ID" value="MESCA000455"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536310_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000455-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20060" checksum="785F3017116C2F38" modified="2013-10-16" version="1">MEKKAQQIYSRQGSNKTLQQSDPARKNNETQSTKPKNPRRRENKNLPSKFEATRQQTNKSRLCLLDKRPKPRGFQQQNHNYEEGASNFNNLPSTSLSSNDIAADYEGSIDCEIEVNSIYNAGSKKQNLNHLLNFNYVSREDSHNIFGRYDGRNSAGTENRTSEKRSITRSSICKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB44</accession>
    <name>T1GB44_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB44"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000465-RA">
        <property type="protein sequence ID" value="MESCA000465-PA"/>
        <property type="gene ID" value="MESCA000465"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760723_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000465-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="7968" checksum="C3895E6BE4E8F3B2" modified="2013-10-16" version="1">MFLIHQVKSKFGLGGFYDEFYGKQVLVITELLKATNDISLKPLTNEMLNTETLSPLENADHSQKPYVSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB93</accession>
    <name>T1GB93_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006683">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA000519-RA">
        <property type="protein sequence ID" value="MESCA000519-PA"/>
        <property type="gene ID" value="MESCA000519"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1588353_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000519-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="18974" checksum="FC74A2DF3878FC75" modified="2013-10-16" version="1">MLDQLVSPTGEQQENKRDILKAAIDQVRSEDLSDFMTKVSKEPHHFHIRYHMIQSLQVTHHKPKDHLSLLGXPLPYDTIPASNPSQTKGPPESPWQESFPPSGYIPEKREELVLLKGGNPDTVLLVWCLSTISGVEQYILPTPQYPQAIMDLIIRTKSTMCLGNVDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBN7</accession>
    <name>T1GBN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBN7"/>
    <dbReference type="STRING" id="36166.T1GBN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000673-RA">
        <property type="protein sequence ID" value="MESCA000673-PA"/>
        <property type="gene ID" value="MESCA000673"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388629_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051660">
        <property type="term" value="P:establishment of centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23058">
        <property type="entry name" value="PDZ2_Par3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052213">
        <property type="entry name" value="PAR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16484:SF17">
        <property type="entry name" value="BAZOOKA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16484">
        <property type="entry name" value="PARTITIONING DEFECTIVE 3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="152"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="211"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="400"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000673-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="711" mass="78826" checksum="3687202B1F6E8FB8" modified="2013-10-16" version="1">MKEVISALASVISGENTTAEISPSTCISFPTKYSKPGCKPHEQSVFYVLNLSSSPNGRPISGYYEYETVQHNVAGTANLARKLSSIPDSPTKTQLHPPQQHPPQSWKPLNGFQSGNLGGSGRSRGPYVTQVHIPHSPPSSTSNGGLYNHHNPQSHASKVAERGRLRRGDRILEINGTKLSGLSDTEIQESLKRSLDCSELRVRVIRGRRIEIALIKGQKGLGFSVTSRDNPAGGDCPKYIKSILPIGAAIEDGRLKPGDRLLEVDGESITDKTQTEVVETLKSKKLGSRVVLVVSRQQELVEEEQENEIKEVNERDIQVSQSNVRSIIESKFEQNTAETPSNDLQIKHSKSQSNIKNIIEQLNENKSESQHFIDAGSESTTSNESQPKNMWKNREILSFNIPVHDSEKAGLGISVKGKKRDSGIYINNVINGGAASRDGRLKPSDLILSVNETSLMNQTNEEAMITLRTAMLKTANHPKYITMTVARNLSRANSSVDISTENSQESSNTSENSGATVIYLTPEKKDRNDLNRKSMPLSSQQNNCWRNESYYMATDDTWSPVRGSKPVGSTRNNNSVLIEDDAEPMSPTIPFRPNDSVGTMSNQTVHDATYSSQLSLDTNTSAEHFSRDAIGRRSISEKHHAALDARETGTYQRNKKLREERDRERRLQQTKSAYFSGSMESITTRIANANKKFDKNHPDLQSEARDVLLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC14</accession>
    <name>T1GC14_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein CLP1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02105613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC14"/>
    <dbReference type="STRING" id="36166.T1GC14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000809-RA">
        <property type="protein sequence ID" value="MESCA000809-PA"/>
        <property type="gene ID" value="MESCA000809"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018195_1_0_1"/>
    <dbReference type="OMA" id="VQYVNCH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01983">
        <property type="entry name" value="SIMIBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.330:FF:000001">
        <property type="entry name" value="Protein CLP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000454">
        <property type="entry name" value="Protein CLP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="5">
        <location>
            <begin position="64"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="3">
        <location>
            <begin position="255"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000809-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40826" checksum="975D765A2F566B78" modified="2013-10-16" version="1">ITGAKVAIFTYRGCTLEVRGETDVIYISRETPMIQYLNCHAALEQMREKAEEDDTLGPIAMVVGPTDVGKSTLCKIFLNYAARMNRRPLYCDIDVGQGGLSVPGMIASILVERPASLIEGFSQSAPLVYHHGHKSPSENNALYKMIIKKMAEVAITSREANKGLKTSGIIINTCGWVKGEGYRSLLHAAEEFQVDVIFVLDQERLYNELLRDVPGKVKVVLLPKSGGVVEISKTQRAELRDSRIREYFYGGRSPLYPFSFDVKWADIKLYKIGAPPLPASCLPIGMKPEDHLTKLVPVSPSPAILHHLISISFAESLEDDVLCTNIMGFICVTAVDPERQTVTVLSPQPRPLPSNNILLLSDIQFMDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCH5</accession>
    <name>T1GCH5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CWH43-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCH5"/>
    <dbReference type="STRING" id="36166.T1GCH5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000989-RA">
        <property type="protein sequence ID" value="MESCA000989-PA"/>
        <property type="gene ID" value="MESCA000989"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690657_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000989-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8813" checksum="9E082E02371D32C7" modified="2013-10-16" version="1">MTTSLCHMLASIKLYEALHPKDQRNESQRKSLKWKKNFFAISIASTIGLLLFFAKHRFYCHDMEDIERITSFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCX1</accession>
    <name>T1GCX1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001144-RA">
        <property type="protein sequence ID" value="MESCA001144-PA"/>
        <property type="gene ID" value="MESCA001144"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834079_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588197" evidence="1">
        <location>
            <begin position="20"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7355" checksum="4724EB08B2C4DBC0" modified="2013-10-16" version="1">MNIFSITFLFASFFALSFGHISINVKQYQKCIYKGMDAKDIAALLGAGRTACKLRDLQIEPAQQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEY2</accession>
    <name>T1GEY2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001907-RA">
        <property type="protein sequence ID" value="MESCA001907-PA"/>
        <property type="gene ID" value="MESCA001907"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2612875_0_0_1"/>
    <dbReference type="OMA" id="NRCYDSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001907-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9225" checksum="BF49D22E8D4E9E9E" modified="2013-10-16" version="1">MTTTLRTNGLHHAVSLGNIQTSSHSADIPSIDNRCYDSEDENIGRKKYHFHHHHHHYNHHKNQWQPKTSAYSKGDIKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFD9</accession>
    <name>T1GFD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Alkaline phosphatase</fullName>
            <ecNumber evidence="2 10">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="2 10"/>
    <dbReference type="EMBL" id="CAQQ02076263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFD9"/>
    <dbReference type="STRING" id="36166.T1GFD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002080-RA">
        <property type="protein sequence ID" value="MESCA002080-PA"/>
        <property type="gene ID" value="MESCA002080"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_4_0_1"/>
    <dbReference type="OMA" id="GDCQAMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF85">
        <property type="entry name" value="ALKALINE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8 10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588247" description="Alkaline phosphatase" evidence="12">
        <location>
            <begin position="17"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="419"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002080-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="495" mass="54830" checksum="D4A60D1781036C98" modified="2013-10-16" version="1">MLRELLLLALASSGLGFKLPSCRNPSECYEERMHPDFEPRIVSRAESSIPKGEETSNFWLDNAKTFVEEQLNRTMNRNYAKNVIFFIGDGMSIPTLAATRNYLGGEEVKLSFEKFPYVGLARTYAVDRIVPDSAATATSYLCGVKANYNTIGINAGVLLNDCNENVANRVTSIAKWSIDAGKSAGVVTTTRVTHASPAGTYASVANRNWENNVEMEEVCGADSSKLNDIAEQLVHGETGKHFKVILGGGKREFIDSKLFEKGRRKGGTIECQVDEIDRLLGLFTNGHMKFHLEDKKQKQPTLAEMTQKAIEMLRKDENGYFLFVEGGKIDIAHHDTSAIYALDETAEFSKAIELARQMTSEEDTLIVVSSDHSHTMSFAGYDERHGDIFGDTNKKADDEKKFLKLSYANGQGFDVYYNTKKNQRRNPKNIDRSSPDAVFPSTLPKESETHGGDDVAVFASGPWSHLFTGAYEQNVLPHLMAYASCVGSGQKSCDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFF1</accession>
    <name>T1GFF1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002092-RA">
        <property type="protein sequence ID" value="MESCA002092-PA"/>
        <property type="gene ID" value="MESCA002092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852245_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7592" checksum="D2792F4BD0D451E8" modified="2013-10-16" version="1">MNLFIPSLSHVFQNIIKLKSLLNITYKIFASIMCQRPKPHAIRICDLSDLPSILMTRRKHTENKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFI9</accession>
    <name>T1GFI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Liprin-alpha CC2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02070300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFI9"/>
    <dbReference type="STRING" id="36166.T1GFI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002131-RA">
        <property type="protein sequence ID" value="MESCA002131-PA"/>
        <property type="gene ID" value="MESCA002131"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068187_0_0_1"/>
    <dbReference type="OMA" id="KECNKAQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057892">
        <property type="entry name" value="LIP-1_CC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF20">
        <property type="entry name" value="LIPRIN-ALPHA, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25526">
        <property type="entry name" value="LIP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Liprin-alpha CC2" evidence="4">
        <location>
            <begin position="154"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002131-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="263" mass="30471" checksum="E238C539D4EE7986" modified="2013-10-16" version="1">EFASITKELTQAREALLERDEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQSVAPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRISLEKNSALEEELTTTKDELQHYKTSTIPAIENGEISNKNGINKEKVNGDTAQEAIENAAKIHELQIIIEKQTSELSQWQRRVSDLNNKISEIDENLSKNQKELVKSQEHCSKLQRDLRENVAQKEDQEERIATLEKRYLSAQRESTSLHDLNEKLEQELRHKEAQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG87</accession>
    <name>T1GG87_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02114398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02114399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG87"/>
    <dbReference type="STRING" id="36166.T1GG87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002399-RA">
        <property type="protein sequence ID" value="MESCA002399-PA"/>
        <property type="gene ID" value="MESCA002399"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034585_4_2_1"/>
    <dbReference type="OMA" id="FHEGHAR"/>
    <dbReference type="UniPathway" id="UPA00753">
        <property type="reaction ID" value="UER00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="138"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="360"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002399-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="430" mass="48732" checksum="D5F163EBAF6B8DBE" modified="2013-10-16" version="1">MASPSLLTNKTTGNYSISSSRKRSFESTITGNPSSMSNSSLELNGVNGGFSTAPTSPVDESDIPTKILATNGHSKSNGFYKHEQPTCSKAVEVKTICKPAPFSHDEEAQKEMERCDYTQKITYKMALEGTAPRPVRVYADGIYDLFHEGHARQLMQAKNVFPNVYLLVGVCNDDLTHSKKGRTVMTEKERYEGVKHCRYVDEIVPDAPWTLDDEFLEKHKIDFVAHDDIPYSAEGCDDLYAQIKARGMFVATQRTDGVSTSDIVARIVKDYDLYVRRNLSRGYSAKELNVSFLNEKKFRLQNKMDEFKEKGRRAFSEVKDDIITKWEEKSREFIDAFLLMFGRERLNNFLKESKEKVIQALSPPGSPSGSVNGDFEDDDDDERYPDFKVDMENNSPQPKRASLRGHQRNGSSISRTSNGKGEDEERRRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGN6</accession>
    <name>T1GGN6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02071865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002559-RA">
        <property type="protein sequence ID" value="MESCA002559-PA"/>
        <property type="gene ID" value="MESCA002559"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1951232_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14400" checksum="BBF1B75207037BF8" modified="2013-10-16" version="1">MVSFGSAQSIRREETKKGSSIAKNRQIIFDPKSVDKIGLKITVSKGFKEITLSNGFKEIAVSNGFKEITVSIRDREITVSIRDTGNHAFEGFSISNKDLIHQLIQHTLSKVSMQSQTIFNTLKSMPKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHI1</accession>
    <name>T1GHI1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHI1"/>
    <dbReference type="STRING" id="36166.T1GHI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002883-RA">
        <property type="protein sequence ID" value="MESCA002883-PA"/>
        <property type="gene ID" value="MESCA002883"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1808407_0_0_1"/>
    <dbReference type="OMA" id="RRFCTDI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002883-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16416" checksum="78026F8492FBEE4B" modified="2013-10-16" version="1">MEAWRRFCTDIEDTNEVFRLRVRSSLVNRVFLLKKLCHLKLGSNTMEAHIGEMLSTVDELKAPGKELKEQLADTMMLGSLPEPYEMLVSALEVRPETELTILDVKEKLIQEHQRHHSVETVVLKTSSPKDNQRKKSSGNSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHV2</accession>
    <name>T1GHV2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003014-RA">
        <property type="protein sequence ID" value="MESCA003014-PA"/>
        <property type="gene ID" value="MESCA003014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815371_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7934" checksum="017426F4BE1D2E9C" modified="2013-10-16" version="1">MENKKQYDKQRKKANTYKLNDVVAILRMQIGSGLKLNLKYLGPYKTTKLPKLEATRDQMQFYLLQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIC6</accession>
    <name>T1GIC6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIC6"/>
    <dbReference type="STRING" id="36166.T1GIC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003198-RA">
        <property type="protein sequence ID" value="MESCA003198-PA"/>
        <property type="gene ID" value="MESCA003198"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654149_0_0_1"/>
    <dbReference type="OMA" id="FNIGHIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013135">
        <property type="entry name" value="Vitelline_membr_Cys-rich-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10542">
        <property type="entry name" value="Vitelline_membr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51137">
        <property type="entry name" value="VM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577387" description="VM domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="VM" evidence="4">
        <location>
            <begin position="107"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003198-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="17283" checksum="5588AB9E69729124" modified="2013-10-16" version="1">MNIKLIISIILVALLEQKSATAEDSFLDPTSSSESSSSSSSDLSARSYSAPSSYSSSYTKSSYLKPSTTYLKPSSHSYPTYSAPSSYSKLSSSYSKAHSSYSGSSSSYAAPHCPTNYIVSCKPSMYPVPCSSYRGSSNYGSSGAYSMKYPMYILPHKYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIL8</accession>
    <name>T1GIL8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sodium-dependent nutrient amino acid transporter 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIL8"/>
    <dbReference type="STRING" id="36166.T1GIL8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003299-RA">
        <property type="protein sequence ID" value="MESCA003299-PA"/>
        <property type="gene ID" value="MESCA003299"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_8_4_1"/>
    <dbReference type="OMA" id="WCIALIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003299-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18478" checksum="22EBBA07C8B8AF86" modified="2013-10-16" version="1">MVSCVITVIKDKFKSIPHWTLSAGASIVGFLCGLVYMTPGGQFIMNLVDFYGCCFIAIFLAIAQLIAVSWMYGVKRLCRDIAFMFGIKTGLYWRICWGFVTPGLMALVLIYSLVEYQPLTYNGVEYPDLYYNIGWGMWAIGICQLPFWACYVVYKQKGSSLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ50</accession>
    <name>T1GJ50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02069235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003492-RA">
        <property type="protein sequence ID" value="MESCA003492-PA"/>
        <property type="gene ID" value="MESCA003492"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349054_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003492-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11310" checksum="CB5DC905F555C10F" modified="2013-10-16" version="1">MVSDDKTNYIWSQAEFTSNYARKRSWVDILERDPKTEISTKLETDSPETEKCFLCCYLMGIKKTVQQYKSLRTTNLVGFNPVEEDNINSLTNTNCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJU9</accession>
    <name>T1GJU9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJU9"/>
    <dbReference type="STRING" id="36166.T1GJU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003753-RA">
        <property type="protein sequence ID" value="MESCA003753-PA"/>
        <property type="gene ID" value="MESCA003753"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1612709_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003753-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="18986" checksum="035220A372FD39E0" modified="2013-10-16" version="1">METFQTAYFAHNIRENLAMKVEVKIRFTLHGKRTTPSVSGKVFGGFVPSKVEYRNPIKKSSFAQSKNSPSFHTKKTTPTVAAKISCFVLQSNGTHFDHNFLFSALRKKNTTPSVAGGHSTFFGLLNTFVHIVMYTYYMFSAMGPQYQKYLWWKKYLTSFQMMLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK13</accession>
    <name>T1GK13_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003824-RA">
        <property type="protein sequence ID" value="MESCA003824-PA"/>
        <property type="gene ID" value="MESCA003824"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818012_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003824-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16220" checksum="B9B7E1555F52A9F4" modified="2013-10-16" version="1">MEKYLNMVYLLEGANEALKRSKEIIKDQELNPLLSSEGTNGGTACHCEKRKLSDGPTSVHKRFQKPSTSAKIRVVANEKQAGKTKTEKSLMRWREKGLVLEIKSTPHSPLNIGFLWEREKVKRVGRTIRFYAFRGVLLMDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK28</accession>
    <name>T1GK28_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GK28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003842-RA">
        <property type="protein sequence ID" value="MESCA003842-PA"/>
        <property type="gene ID" value="MESCA003842"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023545_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22119">
        <property type="entry name" value="F-box_FBXL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047922">
        <property type="entry name" value="FBXL6_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF190">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="181"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003842-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="631" mass="70152" checksum="9C4735319335C3CF" modified="2013-10-16" version="1">MDVQVDSIASTILKYNEPHTTESSSTSPPLDEIPGTSPKNDTQEQELPAMEPLSTXXXXXXXXXXXXXXELPAMEPLSTPPPTGPPKTPPQNTTTSSNKSTKKRKALVALYQSELSPSQGIKICIKKSDTTSTALDISKKRKTKRSRKQKNRDLEDSETQKTVEDDDVNDDTKEQSNWGSTIPEEVLCKIFEYVIESEGPLPTLCRFARVCSLWNRVSLIQKLWSSVDLGTSIKEKFRTELKLKWFIDNRISQCTDLNVGQYFENIFISEFMENKSKNLSIPAHWKVSDVTCVLAKIAYGCKNLKSLTLTGWKGFSSENFKFISEEMPNLSSLDISAINVRNYKITLQTVEMNTSKSAIGLQSLCDAIKTMNGRLTNLHLAHNRLAGMPQIIEALANFCPNLAELDLSNVRTVAHSHGIIHIEKLQAGCQKLKVLRLTNSYVNLSTYGIPGFPDLEELSVAVIGDGRIINDEFLQRILKTSSKLKLLDIRGCARLTHESLIRCPAWDLRHLFLSGCQEMTRDIGSGLELIASKWSHSLIELDLAWANVQKPVDNALKALAEKGVECPISHLNLCGSSVSVEAVREVLIKCPNIKSINLSSCRGLPRGVKRLMSGAAELQELREAINISEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK65</accession>
    <name>T1GK65_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02101466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK65"/>
    <dbReference type="STRING" id="36166.T1GK65"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003883-RA">
        <property type="protein sequence ID" value="MESCA003883-PA"/>
        <property type="gene ID" value="MESCA003883"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1717809_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016263">
        <property type="term" value="F:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033:SF14">
        <property type="entry name" value="GLYCOPROTEIN-N-ACETYLGALACTOSAMINE 3-BETA-GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588369" evidence="7">
        <location>
            <begin position="17"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003883-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17877" checksum="E32B18861EDFEC88" modified="2013-10-16" version="1">MIVLVVIFLAPMSVQMLNMNYAARNLQVDEYVLQEEKSKPKPTKPQFVEVPEEYINPVDRIMSDLNGVIRKYPHHRQGLADKLYDEVKVLCWVNTWPKNHKTRAKAVKETWAKRCNKVLFISTQEDPELDAVALPLKDGRQYLWSKIRGAFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKB2</accession>
    <name>T1GKB2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02010404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02010405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKB2"/>
    <dbReference type="STRING" id="36166.T1GKB2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003932-RA">
        <property type="protein sequence ID" value="MESCA003932-PA"/>
        <property type="gene ID" value="MESCA003932"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075165_1_2_1"/>
    <dbReference type="OMA" id="KGQHETR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF86">
        <property type="entry name" value="CCP84AC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577443" description="Cuticle protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003932-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="16304" checksum="5A8E4702D31B9C48" modified="2013-10-16" version="1">MNSLIIAVISTLAVANAGIVAPVSYTAPLAYTSPLAYSSPLVAKYATAPVVAKTFAAPITVEPEIVDAHPQYQFAYDVQDALTGDSKTQEETRDGDFVRGSYSLIEPDGSRRTVTYTADAINGFNAVVSKDIPYAVAPVVAKQVVAPVVAKQVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL50</accession>
    <name>T1GL50_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004240-RA">
        <property type="protein sequence ID" value="MESCA004240-PA"/>
        <property type="gene ID" value="MESCA004240"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212948_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004240-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12730" checksum="0D3F67AD4482FE09" modified="2013-10-16" version="1">MKFVLYTKRYLICSAAQLEVEYRNDKEKQRWALSVIYKHVFLDHCILTTIPERQQWCTNQVLGVGELENQSVDLIANKEEILMQEEDFFKELLNDNESRTSSQRNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL86</accession>
    <name>T1GL86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ZNF598/HEL2 C2H2 zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL86"/>
    <dbReference type="STRING" id="36166.T1GL86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004281-RA">
        <property type="protein sequence ID" value="MESCA004281-PA"/>
        <property type="gene ID" value="MESCA004281"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1290298_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056437">
        <property type="entry name" value="Znf-C2H2_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23230">
        <property type="entry name" value="zf-C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZNF598/HEL2 C2H2 zinc finger" evidence="1">
        <location>
            <begin position="4"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004281-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="214" mass="24078" checksum="04AAF3FC041503FA" modified="2013-10-16" version="1">MVGDYNALYKHFKESHFVCDQCTEKFTGIFRSDIDLKAHIAHTHAKNLGKLQEKQARTLQLEITLGPRNSRQMETGVAHVRSRADETGPSTATFLVLPPFVSVFFTVAHSVPVDVDSKKRHRSIIDYLTQNQRLGKNDYLIKEIAVDTLMQKGNIGRSISLYVDSQSAIKALESEIVESKVVFECKESISNLNANNSVRVCWIPGHSEFLGNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLA1</accession>
    <name>T1GLA1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reelin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004299-RA">
        <property type="protein sequence ID" value="MESCA004299-PA"/>
        <property type="gene ID" value="MESCA004299"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751813_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577490" description="Reelin domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004299-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17438" checksum="F79844CA6C8E98F1" modified="2013-10-16" version="1">MYQLKYFVLLTLFSSIFGQDEYMEKSNLIDCKHKIYFNPIEKQEFLPFSNFIPISSIKSNFLCDDETVLGTNIFYRGSANFVILLSDEPRNYKKGDTAYEIYVGIYTNAAEIRVRPNTYSNMRRVNNFIGTSFRKDLFAPIEIKLFKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GME4</accession>
    <name>T1GME4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GME4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004718-RA">
        <property type="protein sequence ID" value="MESCA004718-PA"/>
        <property type="gene ID" value="MESCA004718"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148696_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004718-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12871" checksum="B905339D17BBDC1A" modified="2013-10-16" version="1">MYVYIGIGICKVVTGLGNKVSGFLSFSELILLRHCYCGQIVTVLHLPTNIEYTSTLLVLVLWVLNILLSRNIFLCRIHNLNTPQVKCHQEMELHLVLVHKCVTLNWTLYFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNB4</accession>
    <name>T1GNB4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IkappaB kinase</fullName>
            <ecNumber evidence="2">2.7.11.10</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[I-kappa-B protein] + ATP = O-phospho-L-seryl-[I-kappa-B protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19073"/>
            <dbReference type="Rhea" id="RHEA-COMP:13698"/>
            <dbReference type="Rhea" id="RHEA-COMP:13699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.10" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02043354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043355">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043357">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043358">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNB4"/>
    <dbReference type="STRING" id="36166.T1GNB4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005060-RA">
        <property type="protein sequence ID" value="MESCA005060-PA"/>
        <property type="gene ID" value="MESCA005060"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="OMA" id="HIAITEV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008385">
        <property type="term" value="C:IkappaB kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008384">
        <property type="term" value="F:IkappaB kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051180">
        <property type="entry name" value="IKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969">
        <property type="entry name" value="IKB KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969:SF17">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="9"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005060-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="299" mass="34194" checksum="B47144CD2D8771DC" modified="2013-10-16" version="1">MAETKIGEWEKIKNLGAGGFGEVSLWRNSRTEHQVAIKKCKPPIKGSCTPEEEKKLYIRWNGELQLMNKCSTDYIVKGYPELSPPEFLARLNQTCPKELPVLAMEYCPEGDLRAQLQAVENRNGLKECDVQNILFCLRNAVQYLHSQDITHRDIKPENIVIKNISGHKIYKLTDMGYAKNIDSNTIAASLVGTRDYFAPEIITELTYKNTVDFWSVGVIAFEIICGIRPFIPHQQLHTCIIQIGRKSNKHIAITEVPEGGDYRFHENLFSENRCSKVFQKLIESWLCLVLGTREKCEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNK8</accession>
    <name>T1GNK8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02034473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNK8"/>
    <dbReference type="STRING" id="36166.T1GNK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005164-RA">
        <property type="protein sequence ID" value="MESCA005164-PA"/>
        <property type="gene ID" value="MESCA005164"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742969_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF15">
        <property type="entry name" value="CYTOSOLIC PURINE 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005164-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7978" checksum="3ECDE40D954C76ED" modified="2013-10-16" version="1">MTYLFDFPHGAKPDEPHRDWKTYFDIIVVDARKPLFFGEGTILRQVDTSTGALRIGTHTGPLQAGQSFGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPP9</accession>
    <name>T1GPP9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005585-RA">
        <property type="protein sequence ID" value="MESCA005585-PA"/>
        <property type="gene ID" value="MESCA005585"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1429532_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF87">
        <property type="entry name" value="CRESCERIN-LIKE PROTEIN CHE-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005585-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21556" checksum="D82F30066EC2F23C" modified="2013-10-16" version="1">MASVFVYMPYTDWRLRANAICSIEEALKQSDNLLKILPCLDTLLRTLLSSERNMEVAEDKRRVITNLISRLPLDNLEDRTVQILTGLCRQGGPGSNRVSKALMQRLPPAVIVLKLTSDEFLHAKSSRFRENALQMVMYALMTFPSTCFDTTTCVTQVTYAALDRKKRIRQAALAVIAILGQVTSPKWYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPQ7</accession>
    <name>T1GPQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02156233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPQ7"/>
    <dbReference type="STRING" id="36166.T1GPQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005598-RA">
        <property type="protein sequence ID" value="MESCA005598-PA"/>
        <property type="gene ID" value="MESCA005598"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770190_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005598-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16328" checksum="368543243E7564CB" modified="2013-10-16" version="1">MEGKRTYSKDFRNIPFRNFQVQSYYKNYSKTEKPSKCTMEPLKTITANGAYSVNTLTSYAPSLGLSTTSSTTTVSPTNLLPYFDFDVPRNITVTVGQTGFLHCRVERLTDKDCTPLSVVGVTAGLIGPLVVEFIRRGESGIRTIHCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPV1</accession>
    <name>T1GPV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005645-RA">
        <property type="protein sequence ID" value="MESCA005645-PA"/>
        <property type="gene ID" value="MESCA005645"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906690_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005645-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7622" checksum="1D285CD7BDF4B3C7" modified="2013-10-16" version="1">MRKREVENKLITYVIYVRTPTIKEQFRAMNYFDTPEKLIRLCQMILRNTMMHYNAHLSTWIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPV4</accession>
    <name>T1GPV4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPV4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005648-RA">
        <property type="protein sequence ID" value="MESCA005648-PA"/>
        <property type="gene ID" value="MESCA005648"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796909_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005648-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7914" checksum="D2462B5547964E08" modified="2013-10-16" version="1">MYQNRARIAGDRIRSTKTTQQVIDTYGANKNDNFFERHMLLGFLVIAFSIYMVMSYLNIFGLFKNIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPZ5</accession>
    <name>T1GPZ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPZ5"/>
    <dbReference type="STRING" id="36166.T1GPZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005691-RA">
        <property type="protein sequence ID" value="MESCA005691-PA"/>
        <property type="gene ID" value="MESCA005691"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751802_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052285">
        <property type="entry name" value="NEXT_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798">
        <property type="entry name" value="RNA BINDING MOTIF RBM PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798:SF11">
        <property type="entry name" value="RNA-BINDING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="42"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005691-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="17135" checksum="D0F1252CF03516F4" modified="2013-10-16" version="1">MNSMKNESENTPKSSKSQKMVDLTENDQSDEEEEDEEDEEKRTMFCGNLHPKITEALLYELCLQAGPLVKVHIPKPKDGPQKNFGFATYQYSCSVPYAMTFWKESLCLDKKSPSSTETGIQEMARNRKDERGCEKMTTDTTKTIHRRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ20</accession>
    <name>T1GQ20_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005718-RA">
        <property type="protein sequence ID" value="MESCA005718-PA"/>
        <property type="gene ID" value="MESCA005718"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673916_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005718-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8500" checksum="4A3D905D75C63C9C" modified="2013-10-16" version="1">MLAALVSASDNTECRECPPSKFPLCATNGDCYKDFGNICFLEEFNKCKEKNKYSGPDFVKEPFPFCPVDVKRCDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQK9</accession>
    <name>T1GQK9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo repeat-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQK9"/>
    <dbReference type="STRING" id="36166.T1GQK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005918-RA">
        <property type="protein sequence ID" value="MESCA005918-PA"/>
        <property type="gene ID" value="MESCA005918"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028951_0_0_1"/>
    <dbReference type="OMA" id="WICSIDN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 4 ARMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005918-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="51460" checksum="FE17482C28BA0D00" modified="2013-10-16" version="1">MFLDCANPMNLKHLSLVPSWRLILQTDRESLSNDDKESLDMAIAGANALWPLSLSRHNKEIMRKTGIVPLIGRLLKSIHIDVVIPIMGTLQICSSEEKYQLAITHERMIIDIVQHLNSPNVDLKMHCSAAINKCAINELTRDMVREAGGLEPLVQIARDKSVRDNKALLAAATGAIWRCAENAENVKTLDHYSTVKHLIHLLDDESDDVLTNVVGAIAECVKFENNREELRKNGGLPYLVNLLNIACPRLLENLAKTFKECAQDKESMRDPRVQAHAAYALSPCVENALDSGELVRSLVGAMELVVKLLKSKDNFVLAAVCSAIATIAKDPTNLAILTDLKVIYKLANLVNTTDDDLMENLAAAIASCAKHGNNTEELGRLQTVTPIVSFLVSNNAKVHRTAAMALEQLSKDPQNCITMHQSGVVPFLLEAIGSTDRILQLAAAGCLKNIRELALRTEEYLLKINEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQN2</accession>
    <name>T1GQN2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQN2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005943-RA">
        <property type="protein sequence ID" value="MESCA005943-PA"/>
        <property type="gene ID" value="MESCA005943"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1628942_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005943-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="17154" checksum="AC9680F37DC5E732" modified="2013-10-16" version="1">MVLCCSSPVGDASCSRITFSSFDIPSVSEQSLAVDTCGTILGCSLYSTSHIANTPNTGIISSSLFSGINRPIHPLPDIPCELVSGINPNSNLNSDSTEACFLEENTLLILLIATIPWDPVLRNGAQSGTVPSDSSHPSRLPLQHANDLNGENRIPCTSTIATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR87</accession>
    <name>T1GR87_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cornichon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GR87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006164-RA">
        <property type="protein sequence ID" value="MESCA006164-PA"/>
        <property type="gene ID" value="MESCA006164"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112942_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033466">
        <property type="entry name" value="Cornichon_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01340">
        <property type="entry name" value="CORNICHON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006164-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19988" checksum="629A6BEE48985DCD" modified="2013-10-16" version="1">MAFSFSAFTYIFALIGDAFLIFFAIFHVIAFDELKTDYKNPIDQCNSLNPLVLPEYLLHILINLLFLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIAYHVWRYKNRPVMTGPGLYDPTRIMNQDNLSKNMREGWIKLLVYLLSFFYYIYGMVSSLIASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR90</accession>
    <name>T1GR90_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR90"/>
    <dbReference type="STRING" id="36166.T1GR90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006168-RA">
        <property type="protein sequence ID" value="MESCA006168-PA"/>
        <property type="gene ID" value="MESCA006168"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485936_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9793" checksum="4346AAF6F50F6F24" modified="2013-10-16" version="1">MSKQHICCRSPHKTFCPYLATKITPAIPIVGAVLYIFTMSSLLRTTFTDPGVIPRASHDEAAYIEKQIDILIYNGPFDYPCLLTMEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRA7</accession>
    <name>T1GRA7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRA7"/>
    <dbReference type="STRING" id="36166.T1GRA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006185-RA">
        <property type="protein sequence ID" value="MESCA006185-PA"/>
        <property type="gene ID" value="MESCA006185"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906663_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006185-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="6966" checksum="A5A4A442D9E36AC7" modified="2013-10-16" version="1">MLVTLSSVLGSHAFLILVVNFILATVSLALVHERLPDYKPLPDIFLDNVKAQDWALNFTDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS40</accession>
    <name>T1GS40_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006488-RA">
        <property type="protein sequence ID" value="MESCA006488-PA"/>
        <property type="gene ID" value="MESCA006488"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253092_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006488-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12158" checksum="C279A276CF061C01" modified="2013-10-16" version="1">MKSKKNETIELVLNPGRLWLRVHDSLTPKSIFKLWYHQTGTLRTKQITGSVTHAMHQVSPITIIISKDFRANMPSETSVTSWKQTFDWYLPIEDQGFWDNLVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS58</accession>
    <name>T1GS58_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-alpha subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS58"/>
    <dbReference type="STRING" id="36166.T1GS58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006506-RA">
        <property type="protein sequence ID" value="MESCA006506-PA"/>
        <property type="gene ID" value="MESCA006506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1157573_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09562">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037620">
        <property type="entry name" value="LIP-1_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF20">
        <property type="entry name" value="LIPRIN-ALPHA, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="154"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="25991" checksum="28F499DB17852024" modified="2013-10-16" version="1">MAMAESSSGDAASPLTARSIRLERVAQALALSQEELRSRVGMNQYIPSPLGSRVSSQESLRNFNNMGSLGMIQTPTSIVSRDGIVTSVQKKKDSNYDSMSLLGKLSQMPSLTGIPSKISSAGSVDYGRQKKKDHDYRNDLLGEAMKAGTPFALWNGPTIVAWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPQTSRTTLAYGVDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS95</accession>
    <name>T1GS95_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02085388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS95"/>
    <dbReference type="STRING" id="36166.T1GS95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006546-RA">
        <property type="protein sequence ID" value="MESCA006546-PA"/>
        <property type="gene ID" value="MESCA006546"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060241_0_0_1"/>
    <dbReference type="OMA" id="DNEHHIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23277">
        <property type="entry name" value="beta-trefoil_MIR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000005">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715:SF102">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715">
        <property type="entry name" value="RYANODINE RECEPTOR AND IP3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="2" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0407">Ion channel</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIR" evidence="3">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006546-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="283" mass="32119" checksum="8457874C8541C578" modified="2013-10-16" version="1">MRVYLDANGNEGSWFYIMPFYKLRSTGDNVVVGDKVILNPVNADQQNLHVAANYELPDNPGCKEINVLNSSTSWKITLFMEHKENQEHILKGGDVVRLFHAEQEKFLTMDEYKKQQHVFLRTTGRTSATAATSSKALWEIEVVQHDCCRGGAGHWNSLYRFKHLATGYYLAAEADIDTANTTVNITSSNNGDNEHHIKQFRLVSVPYSTDIASLFELDATTMARADSLVPQSSYVRLRHLCSSTWVHATSIPIDIDDDKPVMSKNFVYKENLIFSEENHLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT09</accession>
    <name>T1GT09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006827-RA">
        <property type="protein sequence ID" value="MESCA006827-PA"/>
        <property type="gene ID" value="MESCA006827"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127301_0_0_1"/>
    <dbReference type="OMA" id="CTTLHYY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038976">
        <property type="entry name" value="Ssk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692:SF2">
        <property type="entry name" value="GEO12064P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692">
        <property type="entry name" value="PROTEIN SNAKESKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006827-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13871" checksum="F390DAB2247062DA" modified="2013-10-16" version="1">MYKYFITNIFQGFSVACLVLHFYSFNDGDIITSFLATGTFTGFILIILSVFCGILMRAPIHKRIDIFLSFIGCSLFIASGVLLIQAWESSFRTKTRDLAIIKAALAIVNGVLFGFDALFTFKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT94</accession>
    <name>T1GT94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT94"/>
    <dbReference type="STRING" id="36166.T1GT94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006919-RA">
        <property type="protein sequence ID" value="MESCA006919-PA"/>
        <property type="gene ID" value="MESCA006919"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="OMA" id="WVTENDQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF134">
        <property type="entry name" value="AT07769P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006919-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="21658" checksum="D80DA90659A73465" modified="2013-10-16" version="1">MYVSSYRMNGVDVYLGTWNRGNRSEEGQQRIYAGKRYIINHELYDDDTIVNDIALIKLPAPIFSTTTLPKFDSAGNYKTYEGVLATASGWGDTEDGFSADILRFIEKPISKFSSCNLRYLGGLNSEQQICQQATQKQNTCSGDSGGPLVVFEDGEPILIGATSFGINLGCQSGWPGVFTRITGYLDWIQRHTGIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTC0</accession>
    <name>T1GTC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02106553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTC0"/>
    <dbReference type="STRING" id="36166.T1GTC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006948-RA">
        <property type="protein sequence ID" value="MESCA006948-PA"/>
        <property type="gene ID" value="MESCA006948"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093610_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000135">
        <property type="entry name" value="forkhead box protein G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047208">
        <property type="entry name" value="FOXG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46617">
        <property type="entry name" value="FORKHEAD BOX PROTEIN G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46617:SF3">
        <property type="entry name" value="FORKHEAD BOX PROTEIN G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="5">
        <location>
            <begin position="66"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="3">
        <location>
            <begin position="66"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006948-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="228" mass="24959" checksum="36BE7F48AB66A507" modified="2013-10-16" version="1">MSQIFQSNFSIRSILSSGESSDSTDGMSTPPESPTRMMASSSLSSSPSSSDDEKSSNSSSSSGDSKPPYTYSALIVMAIRNSPEKRLTLSGICEWIADNFPYYQQNKSVWQNSIRHNLSLYSCFVKVPRALDDPGRGSYWMLGPSAEDMTIGETTGRLRRTNNPGVGIRGAANQMHGKMGYNMNPYNQYGPPNAVYFPSPEEVMALQQSSITSNRNRSKCGTITIISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ3</accession>
    <name>T1GTQ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02049476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ3"/>
    <dbReference type="SMR" id="T1GTQ3"/>
    <dbReference type="STRING" id="36166.T1GTQ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007092-RA">
        <property type="protein sequence ID" value="MESCA007092-PA"/>
        <property type="gene ID" value="MESCA007092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033399_0_0_1"/>
    <dbReference type="OMA" id="YWINSKE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF44">
        <property type="entry name" value="ODORANT RECEPTOR 13A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="30555" checksum="438CBB4FC6FF5BB2" modified="2013-10-16" version="1">MGNFSSFLDATALAPCIGFCLLSEFKLFYYWINSKELVHLVEGLQDLFPKTEESQKIYGVEIYLKRVLRLGIVYRCTAAGGYFSCDIFMFNIILLICMNFNYIEANIGKKPKDMDKFIKYHQRVLQISKLAYEVFSIPILVNFISSIFIICFVSFQVTANVPPVELFKYILFFLFEIMQIGTTCFLGEQMATCSRNVGKMAFEQEWWEEDVHFQKKTLFIIERSQKPAILVARGFGAVSLESFSDIMTKSYQFFVCLRHVYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVA1</accession>
    <name>T1GVA1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVA1"/>
    <dbReference type="STRING" id="36166.T1GVA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007691-RA">
        <property type="protein sequence ID" value="MESCA007691-PA"/>
        <property type="gene ID" value="MESCA007691"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1620935_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001268">
        <property type="entry name" value="Blast:Protein CEPU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007691-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="18544" checksum="11A00881B3C449E1" modified="2013-10-16" version="1">MDVICTCALVFRETIELPCKVQNLGSFVLLWRKGTSVLTAGHLKISRDPRFKIVGDYNLQINGVKTQDAGDYICQLGDQENRDQVHTVEILVSYFTLFLMKLRQISANNTFPYFLLSGRLSPLVRSLIEPDSLFTVKEKSFLVSLSKTKRIVSSFDVGVQHSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVD4</accession>
    <name>T1GVD4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-related modifier 1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3/UBA4 homolog. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with cer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3/UBA4 homolog, then thiocarboxylated (-COSH) via the rhodanese domain of the MOCS3/UBA4 homolog.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the URM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02173959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVD4"/>
    <dbReference type="STRING" id="36166.T1GVD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007728-RA">
        <property type="protein sequence ID" value="MESCA007728-PA"/>
        <property type="gene ID" value="MESCA007728"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148208_0_1_1"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01764">
        <property type="entry name" value="Ubl_Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000021">
        <property type="entry name" value="Ubiquitin-related modifier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03048">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14986">
        <property type="entry name" value="RURM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="modified residue" description="1-thioglycine" evidence="7">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" evidence="7">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16431" checksum="9D5B57EC4B7C5710" modified="2013-10-16" version="1">MFTSFEVGKKGLFLEPSLFTDPEHYVKIMGYDKPVDFKLNLTETPQEKPKEKYGGAENLFENIKKREVLLEGGKKWTIRELLQWMKINILKDRPELFLQDDTVRPGILVLVNDSDWELLGELDYEIQQNDNILFISTLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVI2</accession>
    <name>T1GVI2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVI2"/>
    <dbReference type="STRING" id="36166.T1GVI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007788-RA">
        <property type="protein sequence ID" value="MESCA007788-PA"/>
        <property type="gene ID" value="MESCA007788"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120158_0_0_1"/>
    <dbReference type="OMA" id="HELFASM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007788-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11562" checksum="93179F5FC658CF28" modified="2013-10-16" version="1">MTGLDLQGPIFLHEPPHKIEFSNNSGGLIECSGHGSPPPEIEWTPIPPQQDLVFPLQNGSLMFYPFTAEKYRHELHATVYRCKLRNLVGTILSREVHVRGGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVI6</accession>
    <name>T1GVI6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02119738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007792-RA">
        <property type="protein sequence ID" value="MESCA007792-PA"/>
        <property type="gene ID" value="MESCA007792"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2212940_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007792-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12371" checksum="2C5F9CC62C243CF4" modified="2013-10-16" version="1">MAKEENRMKRISTFKMFCCPESSVSKQNRSQDNNIRLPRFLYVKISWISNQTGLWVEDGRNTLVGYVRPDTNPGQLTSCNRLVIPSYSALTRATIKIMKGRQRKIAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWJ1</accession>
    <name>T1GWJ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">inositol-3-phosphate synthase</fullName>
            <ecNumber evidence="6">5.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10716"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="5.5.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02032642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02032645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWJ1"/>
    <dbReference type="STRING" id="36166.T1GWJ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008168-RA">
        <property type="protein sequence ID" value="MESCA008168-PA"/>
        <property type="gene ID" value="MESCA008168"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_998498_0_0_1"/>
    <dbReference type="OMA" id="NNMILEH"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000055">
        <property type="entry name" value="Putative myo-inositol-1-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0398">Inositol biosynthesis</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="279" mass="31195" checksum="2E6F111BA6737F9A" modified="2013-10-16" version="1">MEITNSSINFFILDGWDISSLNIAESMERAQVLDVDLQNQLKETLSKYKPRPSIYDPDFIAANQSDRADNVILKSKYEQFVQIRKDIQDFKKTSGVEKVIILWTANTERFSSVEAGLNTTMFELEDSLRANKSEISPSTIFAMASIAEGCTYINGSPQNTFVPGVIELAEHHGVFIAGDDFKSGQTKLKSVLVDFLVGAGIKPVSIVSYNHLGNNDGKNLSAPQQFRSKEISKSNVVDDMVESNNILYKPNEHPDHVVVIKYVPYVGDSKRPWMNIPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWN7</accession>
    <name>T1GWN7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWN7"/>
    <dbReference type="STRING" id="36166.T1GWN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008215-RA">
        <property type="protein sequence ID" value="MESCA008215-PA"/>
        <property type="gene ID" value="MESCA008215"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340511_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008215-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23582" checksum="0F8EB07F6ECAC1AB" modified="2013-10-16" version="1">MTFLPYVIIIYYDAKLTGALRFFTNLTFTSAFSYAWDYIMRMELQGNKLSFGLVFSEGLTNEFTYAFVMIIMDTFIYAIIGYLIETFFYDEFKFHIVPRSTLDPNTGASLTNISKIYDTQRKAVDDVCLILPRNQISCILGRNGAGSPNLVILDEPCNGIDSRARKYVWDLIKLLRKDRAVFLSTHYLDEAEYLSDSITIMHNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWT5</accession>
    <name>T1GWT5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008265-RA">
        <property type="protein sequence ID" value="MESCA008265-PA"/>
        <property type="gene ID" value="MESCA008265"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973019_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008265-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14631" checksum="DA627D5067864BED" modified="2013-10-16" version="1">MLDSMPIELHLKQLACLCASRLILRKHLRQVFKRGIEAYQRLGKNDYLPQFLLFSVYKGQRRNRAFPGSDIYIEKLVLEIQSKILIFKQNTVLKAAELLISEKIEDKDISIYLDSQAAITAIGSNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWZ7</accession>
    <name>T1GWZ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWZ7"/>
    <dbReference type="STRING" id="36166.T1GWZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008334-RA">
        <property type="protein sequence ID" value="MESCA008334-PA"/>
        <property type="gene ID" value="MESCA008334"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002877_0_0_1"/>
    <dbReference type="OMA" id="TEAEHHY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF5">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="502"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="717"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="401"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008334-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="874" mass="98580" checksum="CA86B40D8FD80BA1" modified="2013-10-16" version="1">MSTGATGTLNAFVDPSSTKRTSILINGDDCYSTVNVTNDTPLYQSSVVVAPTDFNTVTIKCGTGGKKQRTPEEEIIYKNNKINTLLNQKKNLGGKSVIPIGQIKPQGRTLIKLDYEEPKPTTSTETTSTTSTNPDVFSHEEELVKILRNPVEAVRRNLVPHICGKEKSPTVTAPTTTTTTSTVKIEKNSLIGKLLEDPMLHHLADGLESDTVAKLIENSLKRLKANRSALDTSGTKDNDDMEKLINTSLAKIKEERLRLESIAANNHPKILKNKRFNRIEKVLVPELSCSSELTTAGDEDTNSMKNKFYQMLVDATLAEIEISASNDNDYEHQYESIRINGDPIYEEISEIPPPLPLSPPPLLDLDLEKKNAKSIFEGASKYDILSYLVDAKERGIVRETLQEEESDTLTDLNSNEEHPNKPIDIERNDSGVGSETSKSSRSKFQPTGNANQQQPEDDPSNGRNIQIHLCEDCDDPVDTQITESGVMFAPLVCRKCGKKRAERKEIITEIVETEEKYGRDLLIILEEFYRPMLVAGLLTQEQLSAIFLNTEELLENNQALAERMRDALDIALEQGDDDLLTAAASLLLANMEKEKELLRIFLKVSQMENTVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVTPSHLDGRDLLKQSQDKIELHLTHINQEAKDVPTKLWRRISSSSPNRRASCEIDMINIKLRKMAIDTLEWNHDEVRFVLEGKLLLTQPTDGNWKKGRTIKLAPVNALLVTNGKPSANYKADKPLSDQQLSFPKHTGIREASLLLVKEKCGRYTLLREPLYLDKCVVCTEADWDDYFEVHEISSKDTFIFKAEDNIRTKQWYKQLQHHSQGMGAWRKRRNALANIMINGMLSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX73</accession>
    <name>T1GX73_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX73"/>
    <dbReference type="STRING" id="36166.T1GX73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008415-RA">
        <property type="protein sequence ID" value="MESCA008415-PA"/>
        <property type="gene ID" value="MESCA008415"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2225488_0_0_1"/>
    <dbReference type="OMA" id="TMFELKC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030891">
        <property type="term" value="C:VCB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039049">
        <property type="entry name" value="ELOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248:SF4">
        <property type="entry name" value="ELONGIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008415-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9787" checksum="3F49430EF691D61B" modified="2013-10-16" version="1">MFTGIIKFKPNDQMLFNRENILMEDDKTLQEYGITMASAKAQAPCQLGLALRTSSGEFEILEMTPYSAPPDLPEVMKNTEASNGQEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX87</accession>
    <name>T1GX87_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Paf1 complex subunit Cdc73 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX87"/>
    <dbReference type="STRING" id="36166.T1GX87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008429-RA">
        <property type="protein sequence ID" value="MESCA008429-PA"/>
        <property type="gene ID" value="MESCA008429"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061264_0_0_1"/>
    <dbReference type="OMA" id="VXEGASA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032041">
        <property type="entry name" value="Cdc73_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16050">
        <property type="entry name" value="CDC73_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paf1 complex subunit Cdc73 N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008429-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="300" mass="34444" checksum="973E56817653449A" modified="2013-10-16" version="1">MADPLSFLRQYNISKKEIIERDNQIIFGEFSWNKNVKTNYLKYGSGKKGAPREYYTLECLLYLLKNITLQHSVYVRQCAAEEIPAVNRPDRKELLAYLNGETNICSSIDKSAPLEIPTQVKRSIENDGSAIESLSKKARYEEVQVQSFREQLAARLDVNKKEVSVNIDNIKSLSETMSVEKIAAIKAKRLANKRTTIKRNDNDEAMGTELRAILDYDVDSTKDIISRERQWRTRTTVLQSNGKIFAKNIFAILQCIKAREGGGNIRMTSQTAKVPEPIKINKPQPQVAQYNRYDQERFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXA0</accession>
    <name>T1GXA0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008442-RA">
        <property type="protein sequence ID" value="MESCA008442-PA"/>
        <property type="gene ID" value="MESCA008442"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815384_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008442-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="8003" checksum="395D6DD0625C9D74" modified="2013-10-16" version="1">MIVTSCTNNRNSSRERSKEPPLRTNKTRITNFPSMLKFGRVQRNPVNRKYYIFIDIYGLFCVDVFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY69</accession>
    <name>T1GY69_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="1">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02113273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY69"/>
    <dbReference type="STRING" id="36166.T1GY69"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008791-RA">
        <property type="protein sequence ID" value="MESCA008791-PA"/>
        <property type="gene ID" value="MESCA008791"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024661_2_0_1"/>
    <dbReference type="OMA" id="HTAVNIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF24">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="2">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008791-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22323" checksum="C7AB1A6E21F4F984" modified="2013-10-16" version="1">MLLRHKKGASVENRLRDFKNGIIFRRLKQEKPEALDKLVAIPGDISLPMLGIPTKYIKAMENVSLVIHSAATVRFDEPLKAAINLNVSATYEAIKLSKKFPRLDLFCHISTFYSNPNLKFVENKLYPPPMDWQTAIKLSESNIPDETINIFGKKYIQGFPNTYTFTKNLSENVVNDHSHLFPVLIVRPSISKLLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ10</accession>
    <name>T1GZ10_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M16 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ10"/>
    <dbReference type="STRING" id="36166.T1GZ10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009100-RA">
        <property type="protein sequence ID" value="MESCA009100-PA"/>
        <property type="gene ID" value="MESCA009100"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2055586_0_0_1"/>
    <dbReference type="OMA" id="TDVWRIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009100-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13871" checksum="578A78D548F6F663" modified="2013-10-16" version="1">MSDPENVNGLAHFCEHMLFLGTEKYPDEYEYTNYLSKNGGTSNAVTYPTMTKYYFKVAPDKLDGALDRFAQFFIAPLFTESATDREIKAVNSEHEKNLATDVWRIRQVQKHLAADEHPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ21</accession>
    <name>T1GZ21_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ21"/>
    <dbReference type="STRING" id="36166.T1GZ21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009112-RA">
        <property type="protein sequence ID" value="MESCA009112-PA"/>
        <property type="gene ID" value="MESCA009112"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207438_0_0_1"/>
    <dbReference type="OMA" id="MTCQMCG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042797">
        <property type="entry name" value="GRXCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46990">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46990:SF1">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23733">
        <property type="entry name" value="GRXCR1-2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009112-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="52" mass="5780" checksum="B3E67A4FF6DE3D82" modified="2013-10-16" version="1">MTCQMCGGYRLLPCPSCKGSKKSVHRNHFTAEFVALKCMNCDEVGLVKCHNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ33</accession>
    <name>T1GZ33_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009125-RA">
        <property type="protein sequence ID" value="MESCA009125-PA"/>
        <property type="gene ID" value="MESCA009125"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778741_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009125-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="69" mass="7943" checksum="15EE127C44F46A95" modified="2013-10-16" version="1">MNNNNADWVSFPPSVYYYQSYSKLHTKRSIKNSIPYFPPEAALPAEAINSLHTKKQVKILDFVTPETKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ45</accession>
    <name>T1GZ45_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aralkylamine N-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.87</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dopamine + (9Z)-octadecenoyl-CoA = N-(9Z-octadecanoyl)-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51380"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31883"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:51381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dopamine + acetyl-CoA = N-acetyldopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51388"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:125678"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:51389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dopamine + hexadecanoyl-CoA = N-hexadecanoyl-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51376"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:134058"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:51377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>serotonin + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = N-[(5Z,8Z,11Z,14Z)-eicosatetraenoyl]-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:132255"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:51397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>serotonin + (9Z)-octadecenoyl-CoA = N-(9Z-octadecenoyl)-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51392"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:134064"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:51393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>serotonin + acetyl-CoA = N-acetylserotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25217"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17697"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
            <dbReference type="EC" id="2.3.1.87"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:25218"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>serotonin + hexadecanoyl-CoA = N-hexadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51384"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:134059"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:51385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>serotonin + octadecanoyl-CoA = N-octadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:134065"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:51401"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the acetyltransferase family. AANAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.87" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02386251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ45"/>
    <dbReference type="STRING" id="36166.T1GZ45"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009138-RA">
        <property type="protein sequence ID" value="MESCA009138-PA"/>
        <property type="gene ID" value="MESCA009138"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085834_2_0_1"/>
    <dbReference type="OMA" id="PHESVQT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000046">
        <property type="entry name" value="Dopamine N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905:SF1">
        <property type="entry name" value="AT07410P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905">
        <property type="entry name" value="N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588610" description="aralkylamine N-acetyltransferase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="30"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009138-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="234" mass="27018" checksum="A2FC1CC335A86547" modified="2013-10-16" version="1">MKRITFLILSIIKFLNNLTNTSVTYQCSTMEVRVATDKDYDKVLDFIRVHYYKEEPLTIGVEPKQQDQHDEEFNMSSISHGTSVVAVEGDQILGVFLAAPKYSDEAQHIKEEAEKYKMVKDFRNSYHVETQANVFERFGVDKAVHGHVLTVHNEARGKNVGGKLLEKVWEVTKEKGYKLFTSDCTSFYSARLMERMGMILVNEFPYKNYKDSNDNQVFNPPEIHKSIKTFAKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ78</accession>
    <name>T1GZ78_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02118594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ78"/>
    <dbReference type="STRING" id="36166.T1GZ78"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009176-RA">
        <property type="protein sequence ID" value="MESCA009176-PA"/>
        <property type="gene ID" value="MESCA009176"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429581_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF224">
        <property type="entry name" value="RETINOL-BINDING PROTEIN PINTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009176-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10573" checksum="60AC9EFD721C7C03" modified="2013-10-16" version="1">MILKNKIFFIHGNNMDSLYEQIPKEYLPVEYGGNNGSYDDLVKDLENKFYSYRDYFLEEEKFVSNENKRVGGPADGNLFGSEGSFRKLNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ86</accession>
    <name>T1GZ86_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DDB1- and CUL4-associated factor 12 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat DCAF12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02146667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ86"/>
    <dbReference type="STRING" id="36166.T1GZ86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009184-RA">
        <property type="protein sequence ID" value="MESCA009184-PA"/>
        <property type="gene ID" value="MESCA009184"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020124_2_0_1"/>
    <dbReference type="OMA" id="RINEDYM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056151">
        <property type="entry name" value="Beta-prop_DCAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051191">
        <property type="entry name" value="DCAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860:SF16">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23760">
        <property type="entry name" value="Beta-prop_DCAF12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DDB1- and CUL4-associated factor 12 beta-propeller" evidence="12">
        <location>
            <begin position="90"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="DDB1- and CUL4-associated factor 12 beta-propeller" evidence="12">
        <location>
            <begin position="235"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009184-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="324" mass="36986" checksum="2F5BC34BFCF0F7CA" modified="2013-10-16" version="1">YPSCYVKSRLEERKVRARILRAERRRKPDKPDDFLTYEDSDSEDETPHQQEEILNTSYNFAEYLRSRESHLSEERTISLDYTSRYILTHDMLRESHVNLGANYNKVFCSKWLSNRQVVFGTKCNNLLVYDVNMRRTDAIPTLPSNSASHPEVQAGLHSIEVNPSRSFLATGARNSSDIAIYRLPTLDPVCVGEQGHRELIMGLTWLDDQFLVSGSKDSKLSLWRINEDYMDFPDAALSLNGYIHIFNAETFRQKLSRKLPNCQDNVTISYHSEGIYAVGCRSYTILLDARTLQSLKKITSRYSGCGIRSTSFEGNILTVGTGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZH9</accession>
    <name>T1GZH9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02379112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZH9"/>
    <dbReference type="STRING" id="36166.T1GZH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009280-RA">
        <property type="protein sequence ID" value="MESCA009280-PA"/>
        <property type="gene ID" value="MESCA009280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2612863_0_0_1"/>
    <dbReference type="OMA" id="CRMQATG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046620">
        <property type="term" value="P:regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF418">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE WARTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9115" checksum="8D62E4F973A60F3B" modified="2013-10-16" version="1">MDKSMFVQIKAIGLGAFGEVSLVRKIDTTNHLYAMKTLRKADVLKRNQVAHVKAERDILAEADNEWVVKLYYSFQDRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0F1</accession>
    <name>T1H0F1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0F1"/>
    <dbReference type="STRING" id="36166.T1H0F1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009627-RA">
        <property type="protein sequence ID" value="MESCA009627-PA"/>
        <property type="gene ID" value="MESCA009627"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940517_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577283" description="Partial AB-hydrolase lipase domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="2">
        <location>
            <begin position="47"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009627-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14798" checksum="CF476B5FD42E3BBD" modified="2013-10-16" version="1">MNTKRLLIVFLVYFCTPTWTLNSDEEDSSSAERQSKDIREDSRLRVDELITKHGYIGETHNVETEDGYILTAHRIRNDNGKPIFLQHGLIDSSAGYVVMGPNASLGKITNIYFKLGKGFCYGIFYLKSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0N8</accession>
    <name>T1H0N8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0N8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009719-RA">
        <property type="protein sequence ID" value="MESCA009719-PA"/>
        <property type="gene ID" value="MESCA009719"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834085_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009719-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7806" checksum="7C9C115171A22E50" modified="2013-10-16" version="1">MYVSLRKLLYKRLSAKTGDNQLETHHKIFLLIKDKVRLTEIRRIVRSVGLQKSIPCAQFYLVLLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Y4</accession>
    <name>T1H0Y4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Y4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009831-RA">
        <property type="protein sequence ID" value="MESCA009831-PA"/>
        <property type="gene ID" value="MESCA009831"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576570_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009831-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9357" checksum="285B635F5C1D9D7A" modified="2013-10-16" version="1">MKGELIIWTTVIWTQSYRQRSFGQRHLTFGHLDAKCQLSNRYSFGQPIFGQRSDGQEKGRLRTEPDINAMPAATLRTKSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1H124</accession>
    <name>T1H124_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H124"/>
    <dbReference type="STRING" id="36166.T1H124"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009875-RA">
        <property type="protein sequence ID" value="MESCA009875-PA"/>
        <property type="gene ID" value="MESCA009875"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616215_0_0_1"/>
    <dbReference type="OMA" id="RECYSIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000021">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF23">
        <property type="entry name" value="NEURAL-CADHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="128"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="196"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="306"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009875-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="445" mass="49821" checksum="8D2E4A3823FD02ED" modified="2013-10-16" version="1">NSFHPSVLVFQIKAIDYDDNQNGVIRYSINEQGTSNILKLFTINEDTGELFLKKSAEKYENKNFQFFVRATDNGIPSLHSDTPVEIKIVPSSVVLPVFEKKERKPMFIICHTRKIIARVKLAGNVTAKYTLISETSVDDPQFLVNDEGEISLAKTLDREKKDIHVIGVAAEVDGLLATMEISLHVQDENDNAPIFESNPYNLYLAENVEKGTSIMKVTARDLDSGANGELRYYLADGETDGGDVVNIFAIDVYTGWITTLVPLDKETKSEYRFQVIGTDLGNPKHTGKTTVHIKIKDYNDNPPVFKQVNYATSVKEDARPGTVLLQLGVTDKDVDLQTPVEYYIISGDLFSQFQIRESTGDLFISKPLDREQISSYNLGIIVTDGKFTATTNVTVDVLDVNDNPPYCMKYRYRSTINESIQPGSYILKVLAKEQMKISIQNSASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1M7</accession>
    <name>T1H1M7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02070438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02070439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1M7"/>
    <dbReference type="STRING" id="36166.T1H1M7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010092-RA">
        <property type="protein sequence ID" value="MESCA010092-PA"/>
        <property type="gene ID" value="MESCA010092"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_0_0_1"/>
    <dbReference type="OMA" id="DEICTGT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="81"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010092-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="249" mass="27893" checksum="2DE44F5DE6FB4AA0" modified="2013-10-16" version="1">MSNIICFLSDEVRSGKYKELFHPDQLIAGKEDAANNFARGYNTIGRDLIDPVVDRIRKMAEQCNSLQGFFLFHSFGGGTGSGFSALLMDKLCENFAKNISVGDLTSSLFEPDSQMVKCNPGEGKYMACCVLYRGDEFQKIFVDWCPTGFKVGINYKPPCIVPDGDLAPVHRGVSMLASTTAIADAWGRLDHKFDLMYAKKAFVHWYISEGMEADEFGEARENLAALERDYKEVDDDTNNNSSNYEMEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H221</accession>
    <name>T1H221_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H221"/>
    <dbReference type="STRING" id="36166.T1H221"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010251-RA">
        <property type="protein sequence ID" value="MESCA010251-PA"/>
        <property type="gene ID" value="MESCA010251"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2475301_0_0_1"/>
    <dbReference type="OMA" id="MEHIMFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010251-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9975" checksum="4ACB24B638FF0F1C" modified="2013-10-16" version="1">FPLKESISALITKYSSNGYLDILTEKWYGGLPCYKLDTDIIMPRPLGVAAVAGVFLLLGLGMVLGLLILIFEHWFFKYMLPRLRVKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H299</accession>
    <name>T1H299_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase methylesterase 1</fullName>
            <shortName evidence="3">PME-1</shortName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Demethylates proteins that have been reversibly carboxymethylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02194232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H299"/>
    <dbReference type="STRING" id="36166.T1H299"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010336-RA">
        <property type="protein sequence ID" value="MESCA010336-PA"/>
        <property type="gene ID" value="MESCA010336"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024818_2_0_1"/>
    <dbReference type="OMA" id="NHANTIT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022950">
        <property type="entry name" value="PPase_methylesterase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0719">Serine esterase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="120"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR022950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010336-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="32152" checksum="952BF668FA81F1C0" modified="2013-10-16" version="1">MHFFSLSPYNSHVKLVEKVELMMVQPLNQSSSNPRFLELLPSLLGITVIDVVEGTAMEALASMQSFLRSRPNYFRSIPHAIEWCIRSGQVRNVESAKVSMPGQIINVKTGKLSTCELPLPEEEECEKSEELTKFSNPMSISEDEENESSEGFKQPSQVSPENTSKKYTWRIDLSKSEKFWIGWFDGLSEKFLNLRVPKQLLLASIDGLDRALTVGQMQGLYSILFWKKEINLIKFQMQVLARCGHAVHEDRPHEVAEVISSYLIRNKFAEIAGEFRNFMPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2G3</accession>
    <name>T1H2G3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02375098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2G3"/>
    <dbReference type="STRING" id="36166.T1H2G3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010408-RA">
        <property type="protein sequence ID" value="MESCA010408-PA"/>
        <property type="gene ID" value="MESCA010408"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_598923_0_0_1"/>
    <dbReference type="OMA" id="CERYSRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF106">
        <property type="entry name" value="T-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="167"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010408-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="457" mass="50578" checksum="CCB6D56FDCDF2FE2" modified="2013-10-16" version="1">MFPVVKVTASGLEPSAMYEVYLEFVQVDSHRWKYVNGEWVPGGKAEIPPSNPLYRHPESPNFGSHWMKEPISFAKVKLTNKTNGNGQSTHYSTQQSDTPYFMTYEFPETQFIAVTAYQNEEVTSLKIKYNPFAKAFLDAKERPDTMYSHEAHYGWFIAPPGHYATSQSPTASCERYSRTASRSNSRSAPYSTTKPRVVTPSSTPNTTAITPPLSTASALPSSQSGFVPVEPSNSTAVFSYPSNWQSHGQYWSTPPNTNSPIAPNTSPTHSNGSPTNYSNSSPGYTIHHLTPHNQYSSQTNQHLSTATDRFTTTSLWSFFSSPSSLPSNSTSPSHQLYTNVLNPTTTLAYPSTTWHGGAPSAAEYSLYQNTYTYPPEYIPLVSDISAYAQPLEPVEIIKPIDDHSAHHSIYKLEESSVLTLESAPNGNKSVSPKIENISQNTPKENWTPLTPPQSHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2G8</accession>
    <name>T1H2G8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-uridine aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62432"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:16092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82930"/>
            <dbReference type="EC" id="2.5.1.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TDD superfamily. DTWD2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.25" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02377193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2G8"/>
    <dbReference type="STRING" id="36166.T1H2G8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010413-RA">
        <property type="protein sequence ID" value="MESCA010413-PA"/>
        <property type="gene ID" value="MESCA010413"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066458_3_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:tRNA-uridine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DTW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039262">
        <property type="entry name" value="DTWD2/TAPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392:SF0">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03942">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01144">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DTW" evidence="7">
        <location>
            <begin position="19"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010413-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="28906" checksum="03E0631751F2BD59" modified="2013-10-16" version="1">MEEDFEDFLKIDSEPGESARTKCQDCRRPETVCWCSELPKPRLNPSSSIVILQHPHEEKRSLRTALMLQLGLIPGKCLLYKGKRFPNSKSDPDLENILKSTKTLLLYPSPLAVPLQEIEKSEGPYNLVLLDGTWPQAKAIYTSSPMLQNLRQVKIMTTKLSSYIIRTQPTDGCLSTVETAAECLSVLEDDPSYSELLVKPLHILCKYQLDNGAVKHDSKEYLIKNNKYPKLVGRRLENCYALLCNFLFNILIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Q7</accession>
    <name>T1H2Q7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02035987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Q7"/>
    <dbReference type="STRING" id="36166.T1H2Q7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010511-RA">
        <property type="protein sequence ID" value="MESCA010511-PA"/>
        <property type="gene ID" value="MESCA010511"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1717814_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015700">
        <property type="entry name" value="RPC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010511-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="16695" checksum="B99F78306719FAE7" modified="2013-10-16" version="1">MEFARKVKSRIEKTTLGEISSYVEEVYKADMCFLVIKLDLNRIKVLGLEINVDTVIYSICTSKLRVKAALIDPIGASTIIVRIDSAKYGSCLNAELQRLSTAIQNVVVAGLPNISRAVIAIDDTVKPPTYKLCIEGVGLRDVAATYGVIGHHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H300</accession>
    <name>T1H300_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tsg C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="MESCA010608-PA"/>
        <property type="gene ID" value="MESCA010608"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TWISTED GASTRULATION PROTEIN HOMOLOG 1-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12312:SF16">
        <property type="entry name" value="TWISTED GASTRULATION PROTEIN HOMOLOG 1-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tsg C-terminal" evidence="1">
        <location>
            <begin position="73"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010608-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="198" mass="22339" checksum="D00FE3D7097E202C" modified="2013-10-16" version="1">MCKKCLDKHYTECCPCVDMCPTETARNVTESEISEFHGNAAIFDALAKMPDNSLYLTYTNGELIPADRMIEYDEVNDKNCTVLFWNTCMTNKKCEAACENMGAKSSRWFLDGCCQCIGENCIPSGISESKCASCPYDEEEMNETGFDYDTFDEDDLQFGEGTEMMSVKSEVGELPESPVNFTSFVDFYDKMLTIHEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H362</accession>
    <name>T1H362_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="MESCA010678-PA"/>
        <property type="gene ID" value="MESCA010678"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1837388_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010678-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="16260" checksum="D4096BEE23C9AE69" modified="2013-10-16" version="1">MAVPLRIEKDKNVTCDFSDVEECSIEEVEVEFVDEMEMEKSPEVSEPVQEVEIPEPVQELEVPEPNPEVQTKRKFFKSKDRTTKEVNVLEGLKIEVAGSGKMCLKTPKAKKVVRKRKYVKKSGEFLKFKKLKIFQNCLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H363</accession>
    <name>T1H363_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Phoridae</taxon>
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        </lineage>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA010679"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010679-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11654" checksum="B1ED4ACC8FC606A9" modified="2013-10-16" version="1">MKWVKYSFQSSLEAEQPPDHNRISIVIREINQSELLKNLNKQFMNLIHIHYSWRHNVRHMNNLFSVCTSKPNFRGGDESASNKHTRLIESPPTPVKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3D5</accession>
    <name>T1H3D5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3D5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010759-RA">
        <property type="protein sequence ID" value="MESCA010759-PA"/>
        <property type="gene ID" value="MESCA010759"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877779_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577959" evidence="1">
        <location>
            <begin position="19"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010759-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="15442" checksum="CD533FAE095E2BCF" modified="2013-10-16" version="1">MKTQVFFIAIALFAFVQAEQPEDGNTRSLKDWFKKEESKLREFFEKFQTNAKDFIEEAKKGGEEALKAAEEFVKALEGPCNDINKILDTGLIQKIPIKNIKNIARSTKEVCGLIINEGIPKNAEQLQKYIDALKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3J8</accession>
    <name>T1H3J8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fatty acyl-CoA reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3J8"/>
    <dbReference type="STRING" id="36166.T1H3J8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010826-RA">
        <property type="protein sequence ID" value="MESCA010826-PA"/>
        <property type="gene ID" value="MESCA010826"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624837_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF116">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE CG5065-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010826-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="9064" checksum="AD5C859C38685862" modified="2013-10-16" version="1">MMCKKDVIADMVPVDIVINLMITAAWRTANHKSDHMTIYNCCTGKNHPIAWGQFVDYTMTSVRQHPLGYIIFINFQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4G6</accession>
    <name>T1H4G6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02147533">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4G6"/>
    <dbReference type="STRING" id="36166.T1H4G6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011175-RA">
        <property type="protein sequence ID" value="MESCA011175-PA"/>
        <property type="gene ID" value="MESCA011175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329232_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052443">
        <property type="entry name" value="E3_ubiq-ligase_RNF220-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23537" checksum="412282C989DE7678" modified="2013-10-16" version="1">QRIRNNRQNRLRIKIRKRKYGNDFYFLENLFCNSCPICKRKYAMEAGGNLSGISNSSNKMNIDDEQGNKDIEIETVDVESCNDDVPDSQNQPNTQTPVPSESKLESVLYRSACLINKDASDDDVISNSNSWDTNPHISVKNVSELSSTTHNFYNADSCESRNCGSSNREMVDTNNDSDEDVIVDDDESNVKMLIKKRKFAEENTTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4L0</accession>
    <name>T1H4L0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 135 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4L0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011222-RA">
        <property type="protein sequence ID" value="MESCA011222-PA"/>
        <property type="gene ID" value="MESCA011222"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1598663_0_0_1"/>
    <dbReference type="OMA" id="NIKFCLF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011222-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="18503" checksum="7D82EA8DB84DBD4E" modified="2013-10-16" version="1">MFVGKTEEGPIRAQPIPPPEPRSSSNNFNSVSGVVQIYSKFIDSLKRRHECCPHKGNCVHYAVMGGLRPLVGGIGLQILLKLVLSIKKIVQLKLNVKKVFLNRSTIKLGVFLGGFSFLFKAVSCSLRHIVGQDHPLFALPAGFIASTAFFQYPDTTVALYVMWKMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H503</accession>
    <name>T1H503_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H503"/>
    <dbReference type="STRING" id="36166.T1H503"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011376-RA">
        <property type="protein sequence ID" value="MESCA011376-PA"/>
        <property type="gene ID" value="MESCA011376"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1673646_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="18294" checksum="7756CFD4AC75809B" modified="2013-10-16" version="1">PRHFFLLFNITSSIHLLNCVIAIIGAFKLRKWFLIPFIIFEFIRVLLLLTTHIVLIYLLPVFIGYNWSAVVALFQLITLVNSEKYKKLYGDDPMKPIVNSQNGLSTTPEVKMIYTPDYDTVLKKLNKEPKIIAVQPSVNEIAIANYKNWYHRELVGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H533</accession>
    <name>T1H533_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF243 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021680">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="52"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13324" checksum="1A09D1351152DD43" modified="2013-10-16" version="1">APQYSAPQVSAPQYSAPQASGSHFSGSQVSAPHFPAAQASFNSPVAQSHEAPLITKQFYLHSAPEDHSTIQKTKHIVLGRPQKNYRVVFIKNPSSNNANVHLSAEFAPQEEKTVIYCIKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H556</accession>
    <name>T1H556_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="36166.T1H556"/>
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    <dbReference type="OMA" id="CIDSHLA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051070">
        <property type="entry name" value="NF-kappa-B_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680:SF3">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR CACTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011431-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12320" checksum="B5751AF3F5BA2AD2" modified="2013-10-16" version="1">MGGHIDVLRQLVQFGADINSKEGKSGRTPLHIAIEAANDLVLSFLLEECKNTLNIEAITYGGLTAYQFASVLKNAAFQNNLKLRGAEPLTPPDTDDDSDIEDVDVPVQFTNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5K9</accession>
    <name>T1H5K9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5K9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011590-RA">
        <property type="protein sequence ID" value="MESCA011590-PA"/>
        <property type="gene ID" value="MESCA011590"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760690_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038166">
        <property type="term" value="P:angiotensin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009436">
        <property type="entry name" value="AGTRAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06396">
        <property type="entry name" value="AGTRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011590-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8061" checksum="FCC21BD146679A43" modified="2013-10-16" version="1">MTDVNEIIGSPFVRVKLVAFVHFIFICNAMFGSYIVGAYLFYNILFILAMFWSIHCKDSIESIQTLFLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5Y9</accession>
    <name>T1H5Y9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5Y9"/>
    <dbReference type="STRING" id="36166.T1H5Y9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012122-RA">
        <property type="protein sequence ID" value="MESCA012122-PA"/>
        <property type="gene ID" value="MESCA012122"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_2_1_1"/>
    <dbReference type="OMA" id="QEYYRHW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012122-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="152" mass="17127" checksum="8ED3972F2297C174" modified="2013-10-16" version="1">ELNIGLKWPNDIYVNGSVKIGGLNKFDPQSINNAGCALNVGNSSPTTCINDLREFEHYKKKLPHLSYEKTFAIIFNEIEKVLNKVQTGDFDFLYNSYYKIVDKEGTEKKAKVTGIDEYGFLMVQLENGAVETVHPDGNSFDMLKGLILPKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H662</accession>
    <name>T1H662_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H662"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012195-RA">
        <property type="protein sequence ID" value="MESCA012195-PA"/>
        <property type="gene ID" value="MESCA012195"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018373_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20407">
        <property type="entry name" value="Tudor_AKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047367">
        <property type="entry name" value="Tudor_AKAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF65">
        <property type="entry name" value="A-KINASE ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="292"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012195-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="411" mass="45770" checksum="133EE897E4AAD89B" modified="2013-10-16" version="1">RPILYFTLPGVAFIMGVIWLRRNKVRCGDMARMKTRRSQLIQTIIINTNGSCKTSSMEPLVIKIYILIDNNSVTQFLGKSAPINIVPNGKHSSTDQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQLNLIEGINNDVVVSAVISGGHIFVQQPLHPSHPSLVRLQKCLCDNYNNVDTPMLPEIAENGICVCNVDNTWYRVQIVQKDSQDPSRCLVKFSDYGGYMNVGLNDLRQIRSDYLAMPFQATECILSNIAPKDENGWSPKSGEVLYNLTRGVVLQAQVAGYTSDNIPEIYLYASIGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6T6</accession>
    <name>T1H6T6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6T6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012419-RA">
        <property type="protein sequence ID" value="MESCA012419-PA"/>
        <property type="gene ID" value="MESCA012419"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045449_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="entry name" value="Molybdopterin biosynthesis MoaE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23404">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="Molybdopterin synthase subunit MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012419-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="29662" checksum="1CEEEDBD5E3E199B" modified="2013-10-16" version="1">VISLEYEAYESMQKRDICDEIRKKWSDVENIAIYHRLGLVPVKEASVVIAISSPHRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPKSLVQITADEAEISRRIKRFLEMKREQIDYNNIIDFTKSEATDEESTNSCARVDAIFHKQKHSKGHLKIKRVKNESGPQTRPEYTEKLDRLMNIEKRNIKVEGRCFPFGIKERLDNSETFLDIKPTSRSIFERMKTIEDRILYLETISPEYKHFLVRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6U3</accession>
    <name>T1H6U3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L26</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6U3"/>
    <dbReference type="STRING" id="36166.T1H6U3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012425-RA">
        <property type="protein sequence ID" value="MESCA012425-PA"/>
        <property type="gene ID" value="MESCA012425"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_0_1_1"/>
    <dbReference type="OMA" id="VQITKLH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012425-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14465" checksum="88B54110D888B20E" modified="2013-10-16" version="1">NRKRHFTALSYIRRRLMSAPLSKELPQKYNVRSMPVRRDDNQAGKVVQVHLKKFVVYIEKVQHENAYCNTAYVGIHPSKRLIVKLKLDKDRTAILERRGKERLAALGKDKGKYTEETASAPMETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6U4</accession>
    <name>T1H6U4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase</fullName>
            <ecNumber evidence="3">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3"/>
    <dbReference type="STRING" id="36166.T1H6U4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012426-RA">
        <property type="protein sequence ID" value="MESCA012426-PA"/>
        <property type="gene ID" value="MESCA012426"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009193_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.15.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46596">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, dispensable insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00176">
        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="10">
        <location>
            <begin position="561"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="188"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="793"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="64"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="7">
        <location>
            <position position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012426-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="915" mass="105783" checksum="F0D4AB54C7604BFF" modified="2013-10-16" version="1">SKDKHRDKDRDKDKDRHHKSSSSSSSSKHHSDKHRDKDRESSKSSSKDKDRHKSSSSSSSHKSSSKDKERSKDKDQKKGTSSSKHDDKDRDRKKEHKQPSSSKDKDRHHSSSSSSSDRKRDHKSPQSSHDKAIHRESETIRAVATSSQPQKQQIFKEIMIVKPDIKEEKNSQQSEEINVCKQGXKTKPYIKDEPQQHDVSHASSCDYSMTQFDKNEPDFTLKDEPEINESMNNTQQNMEQDEDPEQEEEDSEEDIPLSKRPHHSIKEEEEDDDDSEEDVPLAKKLSDDDDDDDVPLASRQTKPDKNKKKNKRSHKDDDDEDLTVNPRKRLRKATTNASPTKRVKKEKVEEEEHVWRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVTWLASWVENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLHKHIDTIRNNYRDQWKSKEMRVRQRAVAMYFIDKLALRAGNEKDEDQADTVGCCSLRVEHIQLKKQLGEKENVVVFDFLGKDSIRYYNEVEVEKRVFKNLELFMENKKEGDDLFDRLNTAVLNEHLKELMEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKSMENLKEKIRTKQEQIDDCESELHDLKKAAKRGSVKEKVMADKKAKQLDRLKDQLKKLELQETDRDENKTIALGTSKLNYLDPRITVAWCKKHEVPIEKIFNKTQRDKFRWAIHMATEDYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H790</accession>
    <name>T1H790_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H790"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012628-RA">
        <property type="protein sequence ID" value="MESCA012628-PA"/>
        <property type="gene ID" value="MESCA012628"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044393_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47771">
        <property type="entry name" value="LD27203P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47771:SF14">
        <property type="entry name" value="RH73259P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012628-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="30841" checksum="ADD2EF85A0DA09B3" modified="2013-10-16" version="1">PSIPSKTVPVTVEKQVPYPVKVPVPQPYEVVKHVPYPVKEVVKVPVAVPQPYEVVKHVKVPVHVPVDRPYPVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVPYHVEKPVPVPVIKKIPVPVHVKVERPVPVHVDKPVPYHVKVPVPAPYPVVKEIPVPVEKHVPYPVKVPVEKPRPVHIEKHVPEYHEKHVPYKVPEFHHVHHKIEHEHHEHEHEHHHEEIHDFGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H791</accession>
    <name>T1H791_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RCHY1 zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H791"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012629-RA">
        <property type="protein sequence ID" value="MESCA012629-PA"/>
        <property type="gene ID" value="MESCA012629"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192444_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF53">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCHY1 zinc-ribbon" evidence="1">
        <location>
            <begin position="164"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012629-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="233" mass="26240" checksum="ED308782282945E6" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLASGHYQCPTCQTSIIDMSKMWEYLDAQALLIAIPKNLESHLINIFCNDCHTSKSHFHFVGLKCQHCGGYNTTQDVKRRQSWISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7G9</accession>
    <name>T1H7G9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7G9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012706-RA">
        <property type="protein sequence ID" value="MESCA012706-PA"/>
        <property type="gene ID" value="MESCA012706"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_260655_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052752">
        <property type="entry name" value="NACHT-WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF28">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="998"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1040"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1083"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1273"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1275"/>
            <end position="1284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012706-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1312" mass="147551" checksum="37CC88C61E06566B" modified="2013-10-16" version="1">SFRDGSRHAPYFIYGPHGSGKSTLITELYKSVKGWFQSIRVSRIIRFASATPRSAYNLELLRVICQQISIIYDIPEGYLPKDASFDPNYIYNWFQNLLKRIEDFNNELLVVFIDDLHKLNPLDCDIIAALSWLPISLPWNVHIVCSTGVTIEHIKFTQMQKDRFKSKEYYFDMTLDENKTTESMIMLIIYLNLYLNFDHLKCNHGKFVLVDASLITCSEYGLSETELLELLMPTDDPDKLIEIRNGNFNFSSFCQARNEMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCTNGDNTLKIWSLTREEETYKVSHSDDITCFVVTADSLYLITGSRDMSLKVWQANGGKLAQVLVGHSKAVTCVAVSVTNKTQVISGSKDCNLIVWDIHTGEEVHTLAGHLAPVTCVKVSADGTTAVSGSDDKTLIVWETKRGLALTSLQLHVPFIRFDISLECSRILVQLISFTLPVICLHNTPASYIKLPTYSAPRDFKDLRPQGPKRQMKRLLKKEVSLDTYTWQKKYGHLTSSVMMAQVDERLKRRFSVSASMEEISKIAETKTLTSQTNLGPEQAALAQSQHFDQLEALWNKRSPPRRRHHVGLSRQTSLVEDRLESSDEDDYQDERTGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9X2</accession>
    <name>T1G9X2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9X2"/>
    <dbReference type="STRING" id="36166.T1G9X2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000013-RA">
        <property type="protein sequence ID" value="MESCA000013-PA"/>
        <property type="gene ID" value="MESCA000013"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_961633_0_0_1"/>
    <dbReference type="OMA" id="MFAGMEG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000013-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="31331" checksum="9DC9F4349BFDA655" modified="2013-10-16" version="1">ASIKSRPSSSRITAAELEELFQRQQGENADITRFSTMMTTSRFQSGTDSGTTTPPVNGSPMKSSGPIVYGSVAEMKRKKTKNASNGYGTLRGKPCAIPTVGGDLKRFHSTPDLNTKLTGSDVSIWNHAGANSAKGHHSQDDVATLHASLQRLNLPPPTHPPPPPPVGQVVKVDVSRTSEYESTLALQQALKKRAELTTGNKNAEGVASTFKPSTNAKLYASPQELKNMGWKDNTNQQQNNNGTLQRKHVPQSPQPTAQNQSQYARPTIVTNSSQVVAVQPQVQQIYQPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Y1</accession>
    <name>T1G9Y1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Y1"/>
    <dbReference type="STRING" id="36166.T1G9Y1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000023-RA">
        <property type="protein sequence ID" value="MESCA000023-PA"/>
        <property type="gene ID" value="MESCA000023"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640947_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705">
        <property type="entry name" value="AGAP000321-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705:SF1">
        <property type="entry name" value="PNP_UDP_1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000023-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8844" checksum="2A809DE6881DEC61" modified="2013-10-16" version="1">MKLMQKGYDEIQALEVELKILEETSAPPSRPPRDGYIIYCYGDCNKKFEDALIAQKQTGDGDDLMKAIWLDIYKEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAH3</accession>
    <name>T1GAH3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000230-RA">
        <property type="protein sequence ID" value="MESCA000230-PA"/>
        <property type="gene ID" value="MESCA000230"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852248_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000230-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7363" checksum="3D493ED82E008FEB" modified="2013-10-16" version="1">MHSLKNFSNRRRTSYVSYLIGVGGMWCGELGLCCPKFSFALLRTVFLAPLSLVRSCRLCFPMVPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAQ1</accession>
    <name>T1GAQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sarcalumenin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAQ1"/>
    <dbReference type="STRING" id="36166.T1GAQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000310-RA">
        <property type="protein sequence ID" value="MESCA000310-PA"/>
        <property type="gene ID" value="MESCA000310"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1196033_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051943">
        <property type="entry name" value="TRAFAC_Dynamin-like_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681:SF1">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000310-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26194" checksum="655596BD53B224F8" modified="2013-10-16" version="1">MSSAQPPAMYTTSLWSHPYESGAPVRLLRGGGILMWGNKTEVLDGTQLAADYTFSGLQKFGQGLEDRLRGLKLPSKLLEKVNIVEIPGILEVRKQVQRLFPFNDACQWFIDRADMIVLMYDPAKLDADEIIDNPQNYHIYEGLSTLTNISRYDLPDPEVYRDFFRLNPLFEFKKLSDTCTYFGGCPITKLDTAIAYDLPELVGKYKKMAESAVAAVDKKYESLQKDAEKGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBC0</accession>
    <name>T1GBC0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02085775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000551-RA">
        <property type="protein sequence ID" value="MESCA000551-PA"/>
        <property type="gene ID" value="MESCA000551"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485906_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000551-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="10313" checksum="4DF6B30DC634BCCD" modified="2013-10-16" version="1">MNYRRAENRQKLHISPAELLEQRIVLSVCREQNDRKQEQTVTEGAINSSLERFTNKCLKINIYSIPGSKNQFRIRCQYVFRKGVLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC10</accession>
    <name>T1GC10_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC10"/>
    <dbReference type="STRING" id="36166.T1GC10"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000805-RA">
        <property type="protein sequence ID" value="MESCA000805-PA"/>
        <property type="gene ID" value="MESCA000805"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062792_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000805-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="262" mass="30778" checksum="3C70ADB2E4D58CA8" modified="2013-10-16" version="1">MCFRIGCESEEMTQIAYDDRRCSSGNDLWKLNDTLREKDKKVTKDLNEIENIFRRISELQDRFNSLAEEISEVHVFDENTKKIEEDLNKIREKLNRAIAESKDLIKDTREKYTKEQNLLPTDIGQELQALELLSERLQGAMETKEREFKRAKTVRTEYLSGVDEIKQWLQKAEVNVQDRTLEPLKLKEVLQRIGQEITGIYEKLDHVKGNGKIICESSRNSQEKNLVQNTIDQLQQELDQVKYGWMKRNNKLVIVWTLGRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC94</accession>
    <name>T1GC94_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02171124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02171126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000902-RA">
        <property type="protein sequence ID" value="MESCA000902-PA"/>
        <property type="gene ID" value="MESCA000902"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2365581_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000902-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="96" mass="10951" checksum="39BBCFE2E22066A0" modified="2013-10-16" version="1">LSALNIGKKFTTFFLIGKTVLTTNFPRVPVVIGLIFGYDLGLIKAYKGLFWYETILYEPCVYNSVPNFSFSFNHAVTPSVIPMFEDGFWGEENMYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDI9</accession>
    <name>T1GDI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001377-RA">
        <property type="protein sequence ID" value="MESCA001377-PA"/>
        <property type="gene ID" value="MESCA001377"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031681_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001377-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="31920" checksum="18EACAB6A8C23EA4" modified="2013-10-16" version="1">MFNVEMKLNSIESNDLEKKCEKEAEELKGLPIQDKFIKECVSSGTWTITQETVDKAWKECKEETKFSNFQDCLIVNAVHREEMIPQYDCFENKVGINNVGNRYYRWALTDLTHFNSTTAKLFKHSMDCFKKNGESVKEKLEFVQCYSEKLEQDEMKDILLKGWKIPTKEDYEAALEKCKDKVSLKTFEDFKNSKILNDETPSLLKCIAEEFNEFVNGCFYMEREIESNIFYTKKDNREAYKECLRKSLGENDIDFSKYFKCTESLWKVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDZ0</accession>
    <name>T1GDZ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Polycystin cation channel PKD1/PKD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polycystin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02004677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDZ0"/>
    <dbReference type="STRING" id="36166.T1GDZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001543-RA">
        <property type="protein sequence ID" value="MESCA001543-PA"/>
        <property type="gene ID" value="MESCA001543"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_761388_0_0_1"/>
    <dbReference type="OMA" id="FAFTEAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000055">
        <property type="entry name" value="Polycystic kidney disease 2-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046791">
        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF183">
        <property type="entry name" value="AT14535P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20519">
        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin" evidence="17">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="16">
        <location>
            <begin position="69"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin" evidence="17">
        <location>
            <begin position="300"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001543-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="42334" checksum="77C54943E2411303" modified="2013-10-16" version="1">MALNREENIKIIKKLKESEWIDRATRLVSVEFALYNGNTDIFAEVKLAAEILPTGALFPFYTIEAFHVMRLPMQGITFYCFILAIVLPIALKSYIENNEKNEKVYLGLDQLSVSHLVYSNITAMLIFAIWVKVFKYIGFNKTMLQFSTTLKRCAKDLLGFGLMFLIVFLAFAELGVLLFGSQNGDFRDLLTSVTTMLRFILGDFDYTEIEKANRFLGPIYFISYIIFVFFILLNMFLAIVNDTYAEVKGETIEDEDMFWIYLKEVCKKKNTMERYEIKTIKNMLVKTHTYVNEIDYDFEFEDMKTVLDFYQYLETTFLYAIHGHPDVPISLQKERFVDFDNLLLGAPRIRQLRASSGQGCDGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE11</accession>
    <name>T1GE11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001568-RA">
        <property type="protein sequence ID" value="MESCA001568-PA"/>
        <property type="gene ID" value="MESCA001568"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173839_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001568-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12368" checksum="AFBCAF2C323DAC94" modified="2013-10-16" version="1">MRVIHLYEIVVWWKSTCIDSNSQILRKVERTVEVGITGARGTTTSLSVILDILPIDLVAKETAAKADVRLKAIGQSIEKDYEQEINIFTADNQAVLKALETPFLKFYEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEA0</accession>
    <name>T1GEA0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02097254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEA0"/>
    <dbReference type="STRING" id="36166.T1GEA0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001658-RA">
        <property type="protein sequence ID" value="MESCA001658-PA"/>
        <property type="gene ID" value="MESCA001658"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1257334_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053255">
        <property type="entry name" value="EGF-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24047:SF29">
        <property type="entry name" value="EATER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24047">
        <property type="entry name" value="FI01909P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24748" checksum="C3369369AAEEC1F3" modified="2013-10-16" version="1">MLRIIEEKPDDDVEVFDLNEPLMPIGGPYCKEECVNGFCHDGECICIVGYNKVDNGTCQPHCSMGCKNGQCTEPEICECNNGYKPSSRIFDCVPICKSGCAICVQPYTCLVEYLPKIITSTAKPMPTSGTTKAPIEPLTFPSIMKSPTRKPELKPTTIRTEPTTNPKQKSLPKEDADDSKVRIDGKNCEMQKFLETQNLTKDRKLQNIKFVQFWQFEFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEG1</accession>
    <name>T1GEG1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEG1"/>
    <dbReference type="STRING" id="36166.T1GEG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001723-RA">
        <property type="protein sequence ID" value="MESCA001723-PA"/>
        <property type="gene ID" value="MESCA001723"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030068_1_1_1"/>
    <dbReference type="OMA" id="CEKIGYG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328:SF49">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 12-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="393"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001723-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="54085" checksum="A726C1E40E99EA28" modified="2013-10-16" version="1">MSEASTSLLRPQSSSSLNFLQRLGYGFGHVYNDLCAAVWFSYVLLYLQKVRLLPPIEAGALVMLGQISDAVATPVVGWITDKWSTKQKWHMFGTLLIFVSFPLMFSVDPTPADPAQWWWSAVYYGAIVMVFQFAWAMVQITHLSIIPELSLTAKDRHDLSSIRYSASICSNIFVYIVAWIMFKQHTTANSLTQADGYRFRDISLILTLVGMTMTSMFHFSITFSGYAKRRLRSVESVEPQQTFHTTQVDSDTEDLISSTVESQPKLALRNLIRSPRLYQVSFLYVCARLFMTSALVYFPLWLDERATKPTDQPHRTLFNSVILSPMYMMRLEGENIQHLAIVPLVSFFASFVSSVTFKNFGRFIGSEASYLVGSGISIVGCVLIQTISDISLYQLYIIAVVFGAGSAITMVVSLSLTADLVGNNTQYGGFIYSTVTFADKLITGIIVVVVESSKCQHQSDCPHYYRYVLAGLCGISAILGYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEH8</accession>
    <name>T1GEH8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02175723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEH8"/>
    <dbReference type="STRING" id="36166.T1GEH8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001742-RA">
        <property type="protein sequence ID" value="MESCA001742-PA"/>
        <property type="gene ID" value="MESCA001742"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_1_1"/>
    <dbReference type="OMA" id="NSRFCRQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001742-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="44002" checksum="506688F0D7998733" modified="2013-10-16" version="1">MCMIIFIKCLPSTENTWRLHRKYINPCFSLNMLLGFFGIYNKTANELSDVLEKYVGQDASDLRMVFARSMINTSTETTMGKTLEGTKLDLLPQYQICMKAIMTQMFWPWYQYNFIFQFHNLFKPMKAAKKIISRTVNQLIEDNINDTAINNNNNNSESEERPIFIKQAIKLAKENKFTMEDVDAFETTATAIYNALFCLAENTDCQDRLYEEIMDVIPDDIEITYDHIKDMPYLTMTLNESLRLLPPIMLNPREATEDFQLYDKSWIPKGVQFIISIYHLHRNKKYWGPRADEYDPENFSPENVGKRHPFAFIPFSKGARNCIGWRYAQFALKVALIKLIRKYKFTTDYKLKDSVIIPTITMNFQDVPKLKVSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEL0</accession>
    <name>T1GEL0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEL0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001778-RA">
        <property type="protein sequence ID" value="MESCA001778-PA"/>
        <property type="gene ID" value="MESCA001778"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940512_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577314" evidence="2">
        <location>
            <begin position="22"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001778-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14529" checksum="3C3A311C75DBB030" modified="2013-10-16" version="1">MLYFKTSLLVGLGTLSILVASVPTSVLIRDTQLNNNLKAKRSAPSEIPPPSPSNAEPKDEHKSYEVPLKGFEPARKSSPKKRGISNPLQYDDLYLPNRNEEPLYLPGIFHAPVDFQQDRLDLEELPLAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF57</accession>
    <name>T1GF57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF57"/>
    <dbReference type="STRING" id="36166.T1GF57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001986-RA">
        <property type="protein sequence ID" value="MESCA001986-PA"/>
        <property type="gene ID" value="MESCA001986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_0_1_1"/>
    <dbReference type="OMA" id="RVPHDKF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="2"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="32275" checksum="6778F7B2BB807645" modified="2013-10-16" version="1">MHGILDTSATWVLMGPDSGLAYLLSDLGYDVWMGNARGNRYSKNHTKLSIDSQDYWDFTFHEMGKYDLPAVIDFIIKKTGFEQIHYVGHSQGTAIFWVLCSEEPSYTKKIASMHALAPIAFINDMKSPLFRTLVLFLDFITAATKYSESTMLPVMLSHTPSGASIKQLEHFGQLMKSSHFRKFDRGYLRNQIIYGRMTPPDYILANVKVPVALYYAENDLLVSLTGVEKLAKQLPNVIDKYLVPMHKFNHLDFLWAIDAKTLVYDRLVENLQKVEQTFKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFN8</accession>
    <name>T1GFN8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GFN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002185-RA">
        <property type="protein sequence ID" value="MESCA002185-PA"/>
        <property type="gene ID" value="MESCA002185"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940504_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="92"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002185-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14997" checksum="FF63C09C0C8649FA" modified="2013-10-16" version="1">MAQDYECVTTFEPEFVTALKALWGDDELXXXXXXXXXXXXXXXXXXXXXXXXRTSSLLQSKKRISANRFCKIVNELERIEQADYLPTEQDILRARVPTTGILEYPFDLDEWWTSVDSVQKEENGFTALKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFV6</accession>
    <name>T1GFV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067459">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02067460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFV6"/>
    <dbReference type="STRING" id="36166.T1GFV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002256-RA">
        <property type="protein sequence ID" value="MESCA002256-PA"/>
        <property type="gene ID" value="MESCA002256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1654135_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF272">
        <property type="entry name" value="DPR-INTERACTING PROTEIN THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18880" checksum="D48614B0CF22BE23" modified="2013-10-16" version="1">MHRHLLQQQTGGQRSTGRLNYITIAWLRVDTQTILTIQNHVITKNHRMSITHAEKRAWVLRIRDVKESDKGWYMCQINTDPMKSQVGYLDVVEKNPLDNLRSDNQQLYQNPNIVQKIRDKQVICYVQQKYSPQIQLEIEDLVVKPIWLIDDDAKLLNTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGK2</accession>
    <name>T1GGK2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002523-RA">
        <property type="protein sequence ID" value="MESCA002523-PA"/>
        <property type="gene ID" value="MESCA002523"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062793_0_0_1"/>
    <dbReference type="OMA" id="NQIXAYA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685:SF2">
        <property type="entry name" value="825-OAK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685">
        <property type="entry name" value="825-OAK-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588275" description="Cuticle protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002523-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="26984" checksum="7079B2FAAAA3AB81" modified="2013-10-16" version="1">MFKLILVTFAAIAYASAKPGIVAYSAPVVAAAPAIATARSSQYVTRNHNGVAYTSPVKLVASPVAYSGNHVAYTAPVVAAPVAGNFVAAPVSQKLVASPVAYSDHHVAQKLVASDHHVAYTSPVVAAPVTEKFVAASVSQKLVASPVAYSGHHVAQQLVASPVAYSDHHVAYASPVVATPVAEKFVSAPVSQKLVASPVAYSDHHVSQKLVSPVPAVAAPVSQYIASPVAFNSVSSPLTYPYPAYSAPLLASPYNYPASYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH71</accession>
    <name>T1GH71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 6c family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047531">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH71"/>
    <dbReference type="STRING" id="36166.T1GH71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002760-RA">
        <property type="protein sequence ID" value="MESCA002760-PA"/>
        <property type="gene ID" value="MESCA002760"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796875_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.93.10">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIc/VIIs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051389">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034884">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc/VIIs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037169">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48416">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48416:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02937">
        <property type="entry name" value="COX6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81415">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002760-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7371" checksum="53995F753D4666D8" modified="2013-10-16" version="1">MTSTPATASAQKPVLKGLNNAFIKKHLFIAIGLTAVATVAYKFLVNDPRKQAYADFYKKSTLINPSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHD9</accession>
    <name>T1GHD9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHD9"/>
    <dbReference type="STRING" id="36166.T1GHD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002835-RA">
        <property type="protein sequence ID" value="MESCA002835-PA"/>
        <property type="gene ID" value="MESCA002835"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075398_0_0_1"/>
    <dbReference type="OMA" id="IMEYSEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002835-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="320" mass="36395" checksum="1E6BA9F1B1398376" modified="2013-10-16" version="1">MSYSVNGKFLFSGNNITKLPTSLYIFAKAGQLEEIDLSNNQIEEDSLKRIYFKSLTSLKILNLSRNANISSINYSVFKDTQLQNLNMSSVNLKNLNKLGSKNLTILDLSQNSISLIPEDTFEDLENLELLNLGNNKISELQTNVFSSLKKLTKLILNCNVICDIPKGIFKDLSNLVTLDLSYNSLEYIEGLDKLNKLQDLKLQGNYLKVIPSNNELPNSLLKLDISDNPLIFLGRNTFSNLGNLISLDISYTNNSYIKSTFEPLRSLKVLRMHHNNLYNLEDIFFPYKLERLELEFNNLTGVVYKGLETTCSGNYQRSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHG1</accession>
    <name>T1GHG1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002858-RA">
        <property type="protein sequence ID" value="MESCA002858-PA"/>
        <property type="gene ID" value="MESCA002858"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925226_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002858-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6929" checksum="FC7690A9D474A0A4" modified="2013-10-16" version="1">MGGDCLENFILTTIPLENDSSDTLTFLFCLTLKAGFGDFYHDRTYSEQSLVRISTTFSTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHN6</accession>
    <name>T1GHN6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02107554">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHN6"/>
    <dbReference type="STRING" id="36166.T1GHN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002944-RA">
        <property type="protein sequence ID" value="MESCA002944-PA"/>
        <property type="gene ID" value="MESCA002944"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_268884_0_0_1"/>
    <dbReference type="OMA" id="YQRVREM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00029">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042510">
        <property type="entry name" value="CIP2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23161">
        <property type="entry name" value="PROTEIN CIP2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23161:SF2">
        <property type="entry name" value="PROTEIN CIP2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="4">
        <location>
            <begin position="775"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="867"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="11"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="124"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="248"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="716"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002944-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1219" mass="141450" checksum="EA27FE7FADD388DA" modified="2013-10-16" version="1">MFIGYDCDIRIERLLNKIKELQKNIHQTTIEITTLQTSLLKAEQKAQQMDSQSKILVDAKIELEKMKNLLKEKSIELATAKTEVKTLKSSLKIEEEMRLKNEKKITETLAATFKKWEKTKSASDQDYENKLTQKKNEISSINQKLEARENELATRVDECQHLQNKIESYKEMLKQIKDKAMTDKEQFEKNKNELIDSYEKKMIEIRQKLRLEKDSKCRLTMELTSVQSELRDSISSTTSDKEVNDRNVRELRARLNKEIERNNILRNENTSIENKCESLNEENEKLRQELHALSSPYITAHGSLSDLQVIEEQLRSDLAIAKENEDEQRKRASELEKLVGKLEGMLSKFSEQPKSVGDMLEKQNEKLEDKLAAAREQIIVEKQSARTAYMTSYKTEKELEKIKKELEESQQDHNRTKRRMELTEEKIKKVQNEKEQVERNLLDAKKVVLQKEQQIEELKSEINSLKRDVIDKMSTVHKLEENIADLSQKLLQAQRKTETVNMENKRLQKQIKDELSKASFSSDKAEELQTQLKSLSINYDRLKYACEITDSQLTEIEEMLENEQKQHKEKSDKLNKIYKILREKDDIILNLKNESMKLAEEKNVAEMKANNVQLEVDDVRQALEETQKKIINQQQQLIEQSNSLYEVQEKLELFANDSNSMQIMNENLFKEINILKEENTRILTDLYLSKEECLKTSTELKDAIQRINVNPSSILYRTLEEELKREQKKNKVLQDKLDKFERLQYIEKLPIKRIPSKNSVSSAEEAKSPERETQSHRFELTLDAIQGTDLCVVCDKPITSGLAHWKCKECFSKVHRLCRSNLKDNKCIVEPIQELFTPLDDLDFEIAQEPSSDKEYKGDLVLKLEESFGINCVYEVNSKIILLGGSAGLTAFHMDTRNFVTIGGLENVSRICTHHEFSNSIIIANGGERLFQCDLRHLNSRSQSNACTKPKLDTNPLSAKTGACHAIAATTARVVILKFDWEKEYKFRLVKALNLRISSILCTELTAIVSGDKFYEIDLDGDRDSCRDNFTDSYRYEELTDTLTHIRNYEPMAAFKISEEELLLCFMEGGIFLDRYGSKSRPYEVNWQFTPTGFMFQAPLLYVSHYNHVQIIRIHRSYSNDLNTQSYLKEFEGGAAGDGEDVDEHRMKRVLLDFERPKLLASREKLNVYLTSTNKQTDGQELYVLDGLTAFKQNTDYLSMETLSSEATCLTGSIDNFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHT6</accession>
    <name>T1GHT6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002997-RA">
        <property type="protein sequence ID" value="MESCA002997-PA"/>
        <property type="gene ID" value="MESCA002997"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1715352_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588316" evidence="1">
        <location>
            <begin position="21"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17321" checksum="EB85B0B407DAC432" modified="2013-10-16" version="1">MNSRLYIRMWMLWIGDVADCSTLSPAPPTGNDDFLPFGTGIFDFFPGLGLATAFDEADLFPEHSATLRPPPISTPLHVGGGKDVQEYIINRLNCDFKELVEMASFDDQINVQKKDHLTVHCRKKVYQRCEVDCRGTIFLATVANSDYRRTKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHZ1</accession>
    <name>T1GHZ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHZ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003057-RA">
        <property type="protein sequence ID" value="MESCA003057-PA"/>
        <property type="gene ID" value="MESCA003057"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592520_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003057-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9135" checksum="9A99595C054EC06C" modified="2013-10-16" version="1">MGIANFEKLKSDPICKSWKSRNVVEPEARIQYGNGTLKLENLENDNQVSEDPRFFGDEVSETNEDWKSASRSTTMTTLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI39</accession>
    <name>T1GI39_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003106-RA">
        <property type="protein sequence ID" value="MESCA003106-PA"/>
        <property type="gene ID" value="MESCA003106"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199272_0_0_1"/>
    <dbReference type="OMA" id="VTIVMIP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003106-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8131" checksum="0E96463060BD697A" modified="2013-10-16" version="1">MKPNQQSFLKRNLVTIVVVPGLLLAHYGWWKLQYNDKLVSEEERIDMPIFDAAKKVWNKLSKADVKTSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIY4</accession>
    <name>T1GIY4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003421-RA">
        <property type="protein sequence ID" value="MESCA003421-PA"/>
        <property type="gene ID" value="MESCA003421"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2801350_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003421-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7333" checksum="4548D4F7E692B486" modified="2013-10-16" version="1">ETDPEATAPATSRPRAGSLDQFNLRYSQTTSSSLGGGNGSRGFIWLIVCSRTVGYNIKRTPNVNSSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ75</accession>
    <name>T1GJ75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ75"/>
    <dbReference type="STRING" id="36166.T1GJ75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003519-RA">
        <property type="protein sequence ID" value="MESCA003519-PA"/>
        <property type="gene ID" value="MESCA003519"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533970_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF2">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 19, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003519-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9206" checksum="52F2EE6A6EAB6216" modified="2013-10-16" version="1">MSHGGNYTCAPANAKPSSITVHVLRGERPAAMQHSNRSTFEGELSNGITLNTSSTSLFSSSSGIRKTLEIDKLYNLLFLIFFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJF5</accession>
    <name>T1GJF5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02139364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJF5"/>
    <dbReference type="STRING" id="36166.T1GJF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003601-RA">
        <property type="protein sequence ID" value="MESCA003601-PA"/>
        <property type="gene ID" value="MESCA003601"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657311_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="33"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003601-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8163" checksum="31D5D3453B08EFF6" modified="2013-10-16" version="1">MKTTPSPTPSPVPSTTETLTTTKATTPKPSLINRIIPGTVAGWGLTQEQGSTSSLLREVDVPIITNEECKRTKLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP5</accession>
    <name>T1GJP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02031442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP5"/>
    <dbReference type="STRING" id="36166.T1GJP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003695-RA">
        <property type="protein sequence ID" value="MESCA003695-PA"/>
        <property type="gene ID" value="MESCA003695"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313167_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052631">
        <property type="entry name" value="Paired_homeobox_Bicoid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46255:SF3">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46255">
        <property type="entry name" value="SHORT STATURE HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="4">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003695-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11392" checksum="3904F149067774D5" modified="2013-10-16" version="1">SNDLSESSIDNVCIERRSISPIEADSLSNSSVKDQSPSPINVPSRFHQNVSSCVISSKQRRSRTNFTIEQLNELERLFEETHYPEQTNVFFIQLITTFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK58</accession>
    <name>T1GK58_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Otopetrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK58"/>
    <dbReference type="STRING" id="36166.T1GK58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003874-RA">
        <property type="protein sequence ID" value="MESCA003874-PA"/>
        <property type="gene ID" value="MESCA003874"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011508_1_1_1"/>
    <dbReference type="OMA" id="RIFCINT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF30">
        <property type="entry name" value="GH01206P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="137"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003874-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="78517" checksum="667241606D654592" modified="2013-10-16" version="1">MSVPNINVTFAEIDVNDALTNGQFPKPIIRKSNSSGRLTQRRGSDAPSCTNCGHNSSQHPLPISPLAIQHFPKTMDDNRHALLPVSESKANGNMHRNDSGVSINPQPVEFSTSRRPSVMLHDILHARRPSQLFAALKGKPSKGNANDHDGHDDSHRGTHSSSRRESLYPEQAQHSKMKNRRMGDDAITRGLSALYCKFLVILGVAMPVTEIISDKIPTVVYQSFYVYLYGGSILFVVFVYSMTFKNRALFNIIKDYHAKQNNLHVKRKAHRFGSFYLRVGAIAFAIGNMVYSGLEFGQFFELNGDPGCRDPFVAITPILRMVLCIIQVQFIFLNTKDLDMGRHKVISRFGLMHMVATNLCEWLYVLVEETKHEIFHFSQHGDHSAAGSNGTSHGNQIPVLVMISPLNDSIAESNTTDDFMPLGHHLDGCTEQILWEHINKERKYSLKSATEHQQKGTHHFSVDCSQAHRGLFFGILIIVMTIISMIMYFVLYDTPGLRDVATREVTIWEISMFVICSAAIIAAMILMRDLKFVKDNDSHHSMALDCNLLILAQTGVYLYALFSIMGAYYNTIEKQPDRIECIIDEVFGLFQTSIQTLFILNACQRRCKGQQQIRKKPGRDFITFLLVANVSLWFINTLIKGRATFRSGQMDFFGIWAWTIITHTSMPLAIFYRFHSTVCFFEIWKVTYKAKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKF5</accession>
    <name>T1GKF5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKF5"/>
    <dbReference type="STRING" id="36166.T1GKF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003978-RA">
        <property type="protein sequence ID" value="MESCA003978-PA"/>
        <property type="gene ID" value="MESCA003978"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_901047_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042510">
        <property type="entry name" value="CIP2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23161">
        <property type="entry name" value="PROTEIN CIP2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23161:SF2">
        <property type="entry name" value="PROTEIN CIP2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="309" mass="35242" checksum="F4B9BECBEEEC3B46" modified="2013-10-16" version="1">MSRKEMQRQSSLNTASYIQDFSVKSRKYLDHRNEENLVQFNRSASLLCTVKNLQDSLASTFTSLETTNFLIDMYSMLDILSPDLDVFWTCLTVVQQLLKNSKLQQGKSGTSSFIDAGLVLYIKITWEQPYLLQLIRKLVDCIYCDDFDDTIMKLATSVLINLCHKNFCVTCLLLRTVNISSLSKRILSCGVLASKLMIILAEDLDVPSSKDLEKVVTSNFHAIRDSLNTWDVPQLTHIIDFIVSSESNRDFHGAVKNYPNYEENVRDLLQHLSSRSEMEDSCDDGRKNQQACISLVCQLIKYFVTLNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKU9</accession>
    <name>T1GKU9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004130-RA">
        <property type="protein sequence ID" value="MESCA004130-PA"/>
        <property type="gene ID" value="MESCA004130"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_886484_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004130-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="314" mass="34630" checksum="8B352E6B274511B7" modified="2013-10-16" version="1">MKTPLQRILPKTPQTAPARQQNQAQNAVRKKQQHSEDDLDSNIDTSAFGRSRRTPKPNVKYFNDSVVTLKPGKRLKIDDDDEEADIISSDADFEEKDDNASDFDISEEEDVPPAKKTRLSATVATHRQQPQSTPAVQPQRRGVIAPVQKMMVKKPTIVEKTPVSAAPAKKTSIITYTTSNRNTVSRKRPLENSAPQKPATNVQKQVNNNNVQAIKPKPSVVANNKSYQVVRRSTAAAVPATSSSSSTPQIKKEVKKVTPQSKPVPAKKKPEPAPSKKDDTNGDNLEEDIENPMALNTIDISRQCPHLPLLISMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLM5</accession>
    <name>T1GLM5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaoptin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLM5"/>
    <dbReference type="STRING" id="36166.T1GLM5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004435-RA">
        <property type="protein sequence ID" value="MESCA004435-PA"/>
        <property type="gene ID" value="MESCA004435"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_763512_0_0_1"/>
    <dbReference type="OMA" id="WLHIAND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF275">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004435-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="42605" checksum="1E5AA18FBD09DCC6" modified="2013-10-16" version="1">MDKEQIYCGFFHKKLLFATEFNIDFSDLAQSNVSSITLSLPFDNNDENKISEVNNLLVNLTNIKTLKIDQYIEDTYLFNGLDNLENLSWLHIANDSSDYSFVALPKLRNLEIKRLKYKISDRKIIENINIKQIPNLKRLKFFNTYFNKISLDTNNIDHLDLSGNEISSLPEIIFQNKNILKYLNMSFNNIKSIEELISEGFLELKTLDLNNNHIKNVPKIILPKLRYLDLSYNFIEFLANDTFQNGNLSILKINDNFIKKYPTTFFNSMSFLKKLYLSINIQDIKTNNFADCKYLKTLFIDIKITNFNRSKIFLESFLQNCQSIEELHIKVSNAINRRMLLINDLYLKNLQYLKLQILTSTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM15</accession>
    <name>T1GM15_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02173413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM15"/>
    <dbReference type="STRING" id="36166.T1GM15"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004583-RA">
        <property type="protein sequence ID" value="MESCA004583-PA"/>
        <property type="gene ID" value="MESCA004583"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_2_1_1"/>
    <dbReference type="OMA" id="NATERWG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="10">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="11">
        <location>
            <begin position="175"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004583-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="267" mass="30293" checksum="29FDFB8461A5450F" modified="2013-10-16" version="1">MLTLNSEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTSEGLTPDIIINPHAIPSRMTIGHLIECLQGKLGSNKGEIGDATPFNDAVNVQKISTFLQEYGYQLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNYCGLIAIANLRNNTFECKGCKNKTQISQVRLPYAAKLLFQELMSMNIAPRLMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN34</accession>
    <name>T1GN34_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02074680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN34"/>
    <dbReference type="STRING" id="36166.T1GN34"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004973-RA">
        <property type="protein sequence ID" value="MESCA004973-PA"/>
        <property type="gene ID" value="MESCA004973"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_422305_0_0_1"/>
    <dbReference type="OMA" id="THWLEHT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577036" description="Sushi domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="32"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="6">
        <location>
            <begin position="149"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="8">
        <location>
            <begin position="320"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="383"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="596"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="385"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="561"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004973-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="72834" checksum="60CF2FB7B77C7816" modified="2013-10-16" version="1">MFKFLVLFLFIVICNGWRTGDNYSLSSPSSVSLCETPKPVFSQGLRPARKRGRLLQFDCMRGYRMIGSKYLRCKNGKYDGDIPMCAKRGCEMPTRPENSIMLSEYHLSVLRLECNEGYTVVGSRMSVCTGRNWTVPIGSCRETNTPSQLSCDFESEDFCGWTHDDDAEFKWKRHGHFMLAESRGQEINNKARLISPVYNSNHSIGACVRLWYFMHGAAVETLAIHFKTESVEMDSIIDSTDNVIWNVTRESFDGWREAFAHLQEVTESFQIIIEAQVSGSHLGDIAVDDFEIFPCSKDSVEFSTESSDFTESTDSILVVDFESCLDRCSETESRQNTSKVTNTGVEVCDCHQNCVSLNSCCPDYETKCLDEVLLGTTQINDEGETTLPTTTTTVAPITNKPTTTTTTTTTTTTPAPTTRKPITTTTTTTTKKPTTTTTTTTTTPTPATKRIITKPTTTTTTTTTKTTTSTTPAPTTKRNIPISTTSRTEIGTVTAISNRMFDVSTILLPDDENEPDDEGIYDLSVWQILLWSTISAIACFSILFLLINFYKNKYRKFQNEDGFSNNSTKRNSDNSNQLSIRFNTREEQLDFRTAAEVEAGADPEQKPPIADPQKVDESYSDDDDFVESEERRKPYGKLSFPKNRKYSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNE8</accession>
    <name>T1GNE8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNE8"/>
    <dbReference type="STRING" id="36166.T1GNE8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005096-RA">
        <property type="protein sequence ID" value="MESCA005096-PA"/>
        <property type="gene ID" value="MESCA005096"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_3_0_1"/>
    <dbReference type="OMA" id="DSACENH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000004">
        <property type="entry name" value="Pepsin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588402" description="Peptidase A1 domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="62"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="243"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005096-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="353" mass="39424" checksum="0F9CA151385670AA" modified="2013-10-16" version="1">MFKYLAVLCLVVLVSGDLIRIPLQKDETPKSRLQRLANRIRPNRIQNRDGHVELDNYQDDSYYGEITIGTPPQKFKVLFDTGSSNLWVPSTECDASDEACKKHAKYNHKKSSTYKKNGEDFSIQYGTGSLTGFLQTFAEAKSQPGSTFVDAKFDGILGLGFQQIAVDNVVPVFYNMVSQRLVDEPIFSFYLNRKIGASPGGQLMFGGSDRSLYKGEFTYVPVTQEGYWQFNLDKAEVDGNILCENCDAIADSGTTLIAGPVDAIYEIYEAIGADMYGNVDCDSKLPAVDFVLGGKTFRLDGEDYIQKEVQDGETYCFAGFEMNDFWILGDVFMGKFYTEFDLGKKRVGFAEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNR6</accession>
    <name>T1GNR6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02127105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02127106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02127107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNR6"/>
    <dbReference type="STRING" id="36166.T1GNR6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005225-RA">
        <property type="protein sequence ID" value="MESCA005225-PA"/>
        <property type="gene ID" value="MESCA005225"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047969_0_0_1"/>
    <dbReference type="OMA" id="YEFRLVT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF0">
        <property type="entry name" value="PROHIBITIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="3">
        <location>
            <begin position="26"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005225-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="276" mass="30496" checksum="30B7367223A90DC2" modified="2013-10-16" version="1">MAAQFFNRIGQMGLGVALLGGVVNSALYNVEGGHRAVIFDRFSGVKQQVSGEGTHFFIPWVQRPIIYDIRSQPRNVPVITGSKDLQNVNITLRILYRPIPDQLPKIYTILGQDYDERVLPSITTEVLKAVVAQFDAGELITQREMVSLKVSETLTERASQFGFILDDISITHLTFGKEFTLAVEMKQVAQQEAEKARFLVEKAEQQKKASIISAEGDAGAAELLAKSFAEAGDGLVELRRIEAAEDIAYQLSRSRQVAYLPSGQNMLINLPSNLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP40</accession>
    <name>T1GP40_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP40"/>
    <dbReference type="STRING" id="36166.T1GP40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005356-RA">
        <property type="protein sequence ID" value="MESCA005356-PA"/>
        <property type="gene ID" value="MESCA005356"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1724395_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005356-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="152" mass="17248" checksum="42F8339A2E8DADBB" modified="2013-10-16" version="1">MLKNDYLLDSEAGSDSFRKMDMPFSGLSSSVKNVGIVELKKILENLFGGKYKNRDKNPEISRKALKKVAKEAKEARAAKEKAPVSSDKAIPQVDTAFKTFISARFCSAIWAYISDCDETFNYWEPLHYVIYGRGLQTWEYSPEFALGLILIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPC3</accession>
    <name>T1GPC3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPC3"/>
    <dbReference type="STRING" id="36166.T1GPC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005448-RA">
        <property type="protein sequence ID" value="MESCA005448-PA"/>
        <property type="gene ID" value="MESCA005448"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_676688_0_0_1"/>
    <dbReference type="OMA" id="MERSAGM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF73">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="133"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005448-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="407" mass="47303" checksum="74BD73FD02E58A3A" modified="2013-10-16" version="1">MKPRATKEAYRSKRREIVKMSKVEESGDYKCRPKYPSVTNEDECVDFMVEFVDKNHHITKRMVPVGNGIGNNNGYDVGNENSSVVSTTTVSPVTTTENIAQTKTTTTNNYRRRVVYRTTTKRTRTAIEHFERPNITSLTEGHAIVGSSFTFECTLEYDHNIYLKMQWEFPSHVNVNDNRFYISPATYVTPETKEKKKIKSELTINNVHKDDEGIYKCKVLGNNNETHQSQVLTVMEEGENYLEMKTLDGYEKKNYDISIDNFFQLAVEWKSFPHVPHNHIIWFKDGVEIQNDSNYEISIKNKIIIFRINKVSYNDRGTYTLQLNNTLQRKSRNFTFTVIGKPEVLLSDQYVIEGQQAVMDCTVLSTSEPYIVWEYQKCSLTPEWPTCSKYLKNFNITTERIPTNDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPP5</accession>
    <name>T1GPP5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005581-RA">
        <property type="protein sequence ID" value="MESCA005581-PA"/>
        <property type="gene ID" value="MESCA005581"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973020_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14847" checksum="26D0147C15E2FDC6" modified="2013-10-16" version="1">MFPHKQAPLVRVYGLAPAVKDSTTTMLDTIRCSNLNFYIPQELHLPNHPGTCSEYATKTPAKLEKCFYFRIVFCDDRYRRKRNSRLWKNPSLFMNVTSHSYVYDTKFHEGQQATLNICKQRGHTTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQE0</accession>
    <name>T1GQE0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQE0"/>
    <dbReference type="STRING" id="36166.T1GQE0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005844-RA">
        <property type="protein sequence ID" value="MESCA005844-PA"/>
        <property type="gene ID" value="MESCA005844"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073501_0_0_1"/>
    <dbReference type="OMA" id="SGIDSAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="ADAMTS/ADAMTS-like cysteine-rich" evidence="8">
        <location>
            <begin position="28"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="ADAMTS/ADAMTS-like Spacer 1" evidence="7">
        <location>
            <begin position="127"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005844-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="333" mass="37412" checksum="E86189CC7F50D514" modified="2013-10-16" version="1">ESGAKLCHGGDRKYFSCNTQDCPKEEPDFRAQQCSHYDKKPFEGIFYKWVPYMNAPNPCELSCMPEGERFYYRHEPKVIDGTRCNDKSLDVCVNGKCEPVGCDMMLGSSAREDKCRQCAGDGSSCKTVEGLYDANTLQVGYNDILLIPTGATNIEIHEVEPSNNYLALRNLSSHYYLNGNWRIDFPRPMLFAGSWWTYERKPQGFSAPDRLTCLGPTTEPIFLVLLYQDRNVGIQYEYSIPDSNLHNTPDTYTWSQTDFTPCSASCGGGTQTRSISCNSRLTLETVDDSLCDASTKPSEIQRCGMEELPQNGAKVNGVNVQSHVEKMVNRIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR05</accession>
    <name>T1GR05_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the parvin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR05"/>
    <dbReference type="STRING" id="36166.T1GR05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006073-RA">
        <property type="protein sequence ID" value="MESCA006073-PA"/>
        <property type="gene ID" value="MESCA006073"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943499_0_0_1"/>
    <dbReference type="OMA" id="YELMEDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071963">
        <property type="term" value="P:establishment or maintenance of cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21306">
        <property type="entry name" value="CH_PARVA_B_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000015">
        <property type="entry name" value="Parvin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028433">
        <property type="entry name" value="Parvin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12114:SF4">
        <property type="entry name" value="GH23568P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12114">
        <property type="entry name" value="PARVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="20"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006073-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14767" checksum="51A87B552BC5A0C9" modified="2013-10-16" version="1">MKCEKDAFDALFDNAPEKLAIVKRSLITFVNKHLGKLNIEVADLDTEFKDGVYLCFLTGLLGGFFVPLYDYHVTPKTQEQMVNNVALSFELMQDIGLPMPKARPEDIVNMDLKSTLRVLYSLFTKFKDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRI8</accession>
    <name>T1GRI8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02078891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRI8"/>
    <dbReference type="STRING" id="36166.T1GRI8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006273-RA">
        <property type="protein sequence ID" value="MESCA006273-PA"/>
        <property type="gene ID" value="MESCA006273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1002173_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="278" mass="32215" checksum="1F3A16E43009B211" modified="2013-10-16" version="1">MLRKLQTIYGGLSVQKFRSQWQYWLTDTERKLSTGIFATDPDLEEIIHIIIGNTQTWNKYIKQSTNWYEYFPGFLFFTDPACKSFELGTLAQCWINRWANIKNFSNSNFMKHLDKVILYLLESDIHKVLHSIHQIGDNQWFVTHLTDLLYNCGQLDLIGENKLADCEEFRLSLLFDFGSTLMSKESIWLLGINYLEQCGLEGEGAVKTLLPRIPIKNEKQALKVIEVAQERAYVETGNALDWAIRSKDTTYVTSITDFFLDHYSKTGEMLWGGGVHQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRP2</accession>
    <name>T1GRP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">eRF1/Pelota-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRP2"/>
    <dbReference type="STRING" id="36166.T1GRP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006330-RA">
        <property type="protein sequence ID" value="MESCA006330-PA"/>
        <property type="gene ID" value="MESCA006330"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_3_1_1"/>
    <dbReference type="OMA" id="DDLWHLK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006330-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="333" mass="37519" checksum="54A9FA85258E6D4E" modified="2013-10-16" version="1">MHRDQVKSTTIRKVQNESATGSSTSSRIRTTLKIVVESIHFDIQACALRLKGKNIEENQYAEWDTISLERIELACDPSQNADVAAVIMQEGLAQVCLITASMTLLRSKIEMSIPKKRKGFVQQHEKGIVKFYEAVMQGILRHINFDVVKCVIIASPGFVKDQFFEYMNQQAFKNDIKVLLEHKSKFILIHSSSGFKHSLREVLQDPAIISRISDTKALGEVKALEQFYTTLQLEPSKAFYGYNHVEKAAEALAVETLLISDHLFRAPDVVLRKKYVTLVENVRNAGGNVKIFSSIHVSGEQLTQLTGIAAILRFPMPEIEDDDEYIDDNSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRS7</accession>
    <name>T1GRS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02038475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRS7"/>
    <dbReference type="STRING" id="36166.T1GRS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006367-RA">
        <property type="protein sequence ID" value="MESCA006367-PA"/>
        <property type="gene ID" value="MESCA006367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_1_1_1"/>
    <dbReference type="OMA" id="QYQRRHL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="24566" checksum="D1B62BFF39A07365" modified="2013-10-16" version="1">MDLYWIWSIVAIAGKGFSIIAADTRLSSGYSIHTRKQKKTFKLTNNTVLGSTGCWCDTLSLTSLLSYRMQSYVHTHQKTMSTNAIAQLISVMMYNKRFFPYYVSNILAGLDEEGNGVIYSYDPIGHCEKTTYRAGGTAGALLQPEKAISIAIDTFISASERDIYTGDSVVICVITKDGIEEKTENLVTLVVLLLPLTLFHPPQLLYSRDVISSCRAPGPFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS50</accession>
    <name>T1GS50_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7042 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS50"/>
    <dbReference type="STRING" id="36166.T1GS50"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006498-RA">
        <property type="protein sequence ID" value="MESCA006498-PA"/>
        <property type="gene ID" value="MESCA006498"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944326_0_0_1"/>
    <dbReference type="OMA" id="NIDSCTE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055470">
        <property type="entry name" value="DUF7042"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22255">
        <property type="entry name" value="LP06548P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22255:SF9">
        <property type="entry name" value="LP06548P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23069">
        <property type="entry name" value="DUF7042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7042" evidence="1">
        <location>
            <begin position="11"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6637" checksum="EAC61E690C14B524" modified="2013-10-16" version="1">MLKPAVLNLRPFSFTYNRGHGECKSPVSNIDSCTEESRLLLSFQACPDVQGTESTGKVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSL6</accession>
    <name>T1GSL6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Calsyntenin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02163791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSL6"/>
    <dbReference type="STRING" id="36166.T1GSL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006676-RA">
        <property type="protein sequence ID" value="MESCA006676-PA"/>
        <property type="gene ID" value="MESCA006676"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1837387_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045588">
        <property type="entry name" value="CLSTN_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139">
        <property type="entry name" value="CALSYNTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139:SF2">
        <property type="entry name" value="CALSYNTENIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19699">
        <property type="entry name" value="CLSTN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Calsyntenin C-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006676-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="140" mass="15618" checksum="AAD76D592FEBF718" modified="2013-10-16" version="1">MNGEHDDLDFCTVNVFPSLNSDHEELLLENDENLGIDSNVKALISKDGVELKGRDGVTNYVGVLKSLVYTNKKPAYYLNRVFKLSCAQLNAQFKNYEYVLTLTVLHPKQMGPPATVTKSTVSASVKMNVVHDEAPSYHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSQ5</accession>
    <name>T1GSQ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSQ5"/>
    <dbReference type="STRING" id="36166.T1GSQ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006716-RA">
        <property type="protein sequence ID" value="MESCA006716-PA"/>
        <property type="gene ID" value="MESCA006716"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023629_0_1_1"/>
    <dbReference type="OMA" id="NMVQEMT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030719">
        <property type="term" value="P:P granule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF29">
        <property type="entry name" value="FI02030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="53"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="124"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="217"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006716-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="475" mass="53746" checksum="D3E79DB3E17E8547" modified="2013-10-16" version="1">MLRAPTTPTLKLILGLGLCSLGGAMLYAYFKNKDDEDELEGSENDLVTQKPPREITIKMTVTNDCIPLVVGRNGANLKIIEDRTGARIRFRELDSNNQQCEIRGTEKGVADAKSMLMRDIQRSPIVKEEMYVPHSVYTKIIGRCGECLRDICRTSMAKVSVESGDRVESKHPRKIEISGTKAQVNLAKKLIEDKIKEDEEMQRTFNEIEAKREPRRSPTNSMTSLSSSQTSLASIYPQKEKLPFGDVGDKPMEVYVSAIASPSKFWLQIIGPQSRKLDELVTEMTSYYNEKENQEYHKIEDPYLDVVLDLYFVDYGDSEYVSPHDVYELRTDFLTLRFQAIECYLANVKPNLSTDVENWDQQAVSKFEELTQVASWKKLISRVVTYKARPKGGNHIRREGSPLPGVELYDPSEGVEVNIGHMLISHGFAEPDAPVNDVLRLGGGGGWRSPIELNGSNNNWHQFNGNEFTNRLKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTH7</accession>
    <name>T1GTH7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02030549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTH7"/>
    <dbReference type="STRING" id="36166.T1GTH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007009-RA">
        <property type="protein sequence ID" value="MESCA007009-PA"/>
        <property type="gene ID" value="MESCA007009"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123908_0_0_1"/>
    <dbReference type="OMA" id="YESSIGH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007009-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12944" checksum="03946898EA0F5B93" modified="2013-10-16" version="1">MGTLGTHVKSGHADFYPNGGVSPQSNCVLTSHARHQKKRAITCSHSSAIRFFKLSFNPEYAFIGYQCSSYRMFVFGLCKNNKKSQFGVHSTREEGDFYLTTEVVFPYVLRITYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV13</accession>
    <name>T1GV13_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007590-RA">
        <property type="protein sequence ID" value="MESCA007590-PA"/>
        <property type="gene ID" value="MESCA007590"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909078_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007590-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15474" checksum="109CA382449A3018" modified="2013-10-16" version="1">MIENDRLPELAIVQGSINECVKNATEDGSWMFTSVKYTLKQAREENNYIRRTTDTCVTSYPSGYKLTDCVNDRLQRGNNNVWDLLYKTDKDIQVALDQYDNIGRQAMECTFNVVENFSRDIEDVLRTLEKCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV24</accession>
    <name>T1GV24_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mucin-4-like C8-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV24"/>
    <dbReference type="STRING" id="36166.T1GV24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007603-RA">
        <property type="protein sequence ID" value="MESCA007603-PA"/>
        <property type="gene ID" value="MESCA007603"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888148_0_0_1"/>
    <dbReference type="OMA" id="CAYDYAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056619">
        <property type="entry name" value="C8-3_MUC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF52">
        <property type="entry name" value="MUCIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23263">
        <property type="entry name" value="C8-3_MUC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mucin-4-like C8-3" evidence="4">
        <location>
            <begin position="91"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007603-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15581" checksum="C1C48AB00827C344" modified="2025-10-08" version="2">MFGKWSFDPTDDFVLPDGSSINLNTNNFQQIYYNFGLKWMLADRDIPGVGKSLFTQEFGRGASYYNNASFVPQFGKYPQEFLPANRSYDIQRAEELCGESYQCAYDYAMSQNRDMAHFTKNNFDAAVNLQNLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV41</accession>
    <name>T1GV41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV41"/>
    <dbReference type="STRING" id="36166.T1GV41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007623-RA">
        <property type="protein sequence ID" value="MESCA007623-PA"/>
        <property type="gene ID" value="MESCA007623"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064865_1_0_1"/>
    <dbReference type="OMA" id="TDFKTMF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14366">
        <property type="entry name" value="CUE_CUED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040195">
        <property type="entry name" value="CUE_CUED1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040192">
        <property type="entry name" value="CUEDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13467">
        <property type="entry name" value="CUE DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13467:SF3">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="7"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007623-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="30775" checksum="EBDF9610222840A0" modified="2013-10-16" version="1">MSTVQLDFAQAMTDFKTMFPDMDSDVIEAILRANQGAVDATIDQLLQMSTDNQNEKLRNELDKSSENASGTENLLIDTRDLPDILLIDKTGTSQSPSTNSQHLPELLKTSRNLSTGASPKVKQTPKEAHNSKWKPPLLGPLPPDFLRITNDGRTTDYDLPDEQFALMLQNEEFMNELRWNQEFMNALEKEQQEKGRHDVDDAAFKERLRHMGKVSRKKFLQMARVFTWQRRNKNKPTSAKQIDSQPLEESDDEDHHHHQSKSRVISFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV93</accession>
    <name>T1GV93_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02060829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV93"/>
    <dbReference type="STRING" id="36166.T1GV93"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007683-RA">
        <property type="protein sequence ID" value="MESCA007683-PA"/>
        <property type="gene ID" value="MESCA007683"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_931506_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007683-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="299" mass="34624" checksum="9A5E94043ABA1C1A" modified="2013-10-16" version="1">MFHYEGGWPKDINFLDEEQTLRHRKKIERDDIWGQKVILLTKPAMKAIHQNSAVNVYEEYFEDMEILELRSSFEARLLNVYHDLLYDEKRVVGSLQWCSHPKTHILSVLWNTIFPPISSPMDVKKFDFYIWDADNPLKPINIFHSPYAVHQALYCPKDPEIIAGCMRSGMVGLWDTRVPGGNPFAVSPLEASHTDVASCICWVHSKTNMEFYSGSIDGTVKYWDGRNLNHVMQEFFIDPVRTDEQDSKRSMGATVLEFEYTIPVKYNIATEHGMFFVGNRKGLTPLETFTAIYRKMIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVU4</accession>
    <name>T1GVU4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor L-glutamate and glycine-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02087542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02087543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02087544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVU4"/>
    <dbReference type="STRING" id="36166.T1GVU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007911-RA">
        <property type="protein sequence ID" value="MESCA007911-PA"/>
        <property type="gene ID" value="MESCA007911"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1679951_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007911-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="157" mass="18237" checksum="E38029E4D1A1A6BC" modified="2013-10-16" version="1">MYREGLRGEVLEGNAKYEGYCVDLIRKLAKISNFEFTFVEADSNGKYMKEKREWDGIIGYIIDRVVLSNFGAEITQDNLFKSGYNSLNNTINPSDNNHHRIPRTTKKLILIKHRSAFHHFGVSRATGNFDCEATGYFLKARSEAEPMQLKRLRHMKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVV5</accession>
    <name>T1GVV5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02022400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVV5"/>
    <dbReference type="STRING" id="36166.T1GVV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007922-RA">
        <property type="protein sequence ID" value="MESCA007922-PA"/>
        <property type="gene ID" value="MESCA007922"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1697544_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007922-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="18150" checksum="7055EEE8A83C518F" modified="2013-10-16" version="1">MCYLLFPDIGLRHKESRWGDSLFWRSVVLLLGFFFQLPDFVKLIMVTYIVRFLSISPCFVKSFLKNISPTVIEKVEFLRNISDNNIEKLDGDFIKGNSGILKFYCGKDDQNTQTVLKELLPEQNLFVDQDDVGEHFTVYHSFKMAEEVKKMMMET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVW3</accession>
    <name>T1GVW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02081897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007931-RA">
        <property type="protein sequence ID" value="MESCA007931-PA"/>
        <property type="gene ID" value="MESCA007931"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973013_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007931-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14779" checksum="65FBC92FD0F4E1A8" modified="2013-10-16" version="1">MKPNLEDCMLCRWGINSKSKFPDTLSELLQSLLRVAKDIVFLSWFVCKCGSLQAKKQIIEDIDIILCECQALLGSRLDELGAYFLLPEELRDIRTTNIIRLVRKFRCVRNDNEGSLWTILMCLRMCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWD2</accession>
    <name>T1GWD2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02158682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02158683">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWD2"/>
    <dbReference type="SMR" id="T1GWD2"/>
    <dbReference type="STRING" id="36166.T1GWD2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008105-RA">
        <property type="protein sequence ID" value="MESCA008105-PA"/>
        <property type="gene ID" value="MESCA008105"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160903_0_0_1"/>
    <dbReference type="OMA" id="WIICMTI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008105-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11450" checksum="86224AD63050A138" modified="2013-10-16" version="1">MLKLYYIVYAVASPLKIFLTCYYGTAFQEECDNLTKSIFACNWVYQTRNFKNDLKMFLQGSLRTLKFSAGGIMTISLNGFLQVMKSAYSLFALLNHMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWI3</accession>
    <name>T1GWI3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02193368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWI3"/>
    <dbReference type="STRING" id="36166.T1GWI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008160-RA">
        <property type="protein sequence ID" value="MESCA008160-PA"/>
        <property type="gene ID" value="MESCA008160"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1699912_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008160-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17344" checksum="0280ADB249A8B3F2" modified="2013-10-16" version="1">MNGGICVDGVDSFWCSCPPATTGLLCECHISDYNMECGNFTLVSVDPFSTTSSSLDNVYTLNSTKIPETETKYDIDADLKTDYFDSIYPVTLPTEDQSVETIVFTGFPYETEATTTEIISKHSSEEYYTNSESLEYYDLHTHEETTEIQTTSKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWJ6</accession>
    <name>T1GWJ6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02020520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWJ6"/>
    <dbReference type="STRING" id="36166.T1GWJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008173-RA">
        <property type="protein sequence ID" value="MESCA008173-PA"/>
        <property type="gene ID" value="MESCA008173"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099972_0_0_1"/>
    <dbReference type="OMA" id="SAWQDQD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15852">
        <property type="entry name" value="SNARE_Syntaxin8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041875">
        <property type="entry name" value="Syntaxin-8_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF124">
        <property type="entry name" value="SYNTAXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="77"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008173-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="166" mass="19518" checksum="8AA3ECCE3BFF3C83" modified="2013-10-16" version="1">FRTVEETERRQRQVETLRSNLLTLKTRFLKLTRNDREELIQQNRQNSLWEDDDDVPIIPTSSMNSNSNALNIRDTQIRIMEEQNRGLDVLSQTISRQRELASRLGNEVVDQNHILDNIADNMDRLDGRVTQETDRIAVITRQDKTWGYWMVIIILFIAIILVTVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWL5</accession>
    <name>T1GWL5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWL5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008192-RA">
        <property type="protein sequence ID" value="MESCA008192-PA"/>
        <property type="gene ID" value="MESCA008192"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796864_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008192-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7700" checksum="F228D54777AB5EF8" modified="2013-10-16" version="1">MEFVFRIERPSTSLAGEESASRGENNWIPTIGISTHIKRRESKTRGSTLITYILPNILLSELPLNLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWV3</accession>
    <name>T1GWV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Formate--tetrahydrofolate ligase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWV3"/>
    <dbReference type="STRING" id="36166.T1GWV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008285-RA">
        <property type="protein sequence ID" value="MESCA008285-PA"/>
        <property type="gene ID" value="MESCA008285"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138778_1_0_1"/>
    <dbReference type="OMA" id="RDELIPW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="13850" checksum="7CBC676BD7D13115" modified="2013-10-16" version="1">MFHEATQSDEGLYERLFPVPNDITIARSQQPKDISILAEEIGLISNEVSLYGNKKAKISLSVKDRLKDRQDGKYVVVAGITPTPLGEGKTTTLIGLVQALCAHKSRNTIACLRQPSQGPTFGIKGGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX63</accession>
    <name>T1GX63_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008405-RA">
        <property type="protein sequence ID" value="MESCA008405-PA"/>
        <property type="gene ID" value="MESCA008405"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443369_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008405-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10642" checksum="199CFF145D79CF06" modified="2013-10-16" version="1">MKSRDLRKSPLQRLSICRAVAPIEKKIPIKNRRKTNWESFKSEKDLSCYYCLKQFQGCIRRDEIDVIERTAPEVIDKFLAIEKTTNSLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXY2</accession>
    <name>T1GXY2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">STL11/RBM22-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXY2"/>
    <dbReference type="STRING" id="36166.T1GXY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008695-RA">
        <property type="protein sequence ID" value="MESCA008695-PA"/>
        <property type="gene ID" value="MESCA008695"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027112_4_0_1"/>
    <dbReference type="OMA" id="PYYRRNK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="STL11/RBM22-like N-terminal" evidence="2">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008695-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="31253" checksum="EE223DB855EFA9B3" modified="2013-10-16" version="1">MSMSKTTNTYNRQNWEDSEFPILCQTCLGDNPYVRMIKEKYGKECKICSRPFTIFRWCPGARMRFKKTEVCQTCARLKNVCQTCLLDLEYGLPIQVRDAALKIADQIPQSDVNKEYYIQNVDAEVSNTDGTQAAGTVGKSLAPSEILSKLARTAPYYKRNRPHICSFWVKGECKRDRYYGRNDPVAEKILKRAATLPSLETPEDKTITTLYVGNLHEDITEPEVRDNFYHISNTAHPRFKPTKVLFSISNEMKRILDKERWMERNKTPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY45</accession>
    <name>T1GY45_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY45"/>
    <dbReference type="STRING" id="36166.T1GY45"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008765-RA">
        <property type="protein sequence ID" value="MESCA008765-PA"/>
        <property type="gene ID" value="MESCA008765"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_994953_0_0_1"/>
    <dbReference type="OMA" id="IDIFWEA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF186">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008765-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="280" mass="31560" checksum="3543938C7160D734" modified="2013-10-16" version="1">MEDMRDPALRKSYYHGVGGPITVENYKFNSPLIDIFWEAVKELKVENLEGDINGKSQTGFTKPHATVRNGLRCSANKGYLRPASKRPNLDIMINAFVRKINIDPETKVANSVEFDLFGLPHTAHSHREIILSGGAINSPQILMLSGVGPRDHLQEFDIDVIKHAPGVADGGMIGRTASGVSVDNYANVFEPVIYKDSYIITPLLMRPRSRGRIRLQSILPRKHPLIYANYFKDPYDLAVLVEGLKYAHAFSQTKILRYLNATLNIYSWRNFGCFLIILIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYC1</accession>
    <name>T1GYC1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02184766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYC1"/>
    <dbReference type="STRING" id="36166.T1GYC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008845-RA">
        <property type="protein sequence ID" value="MESCA008845-PA"/>
        <property type="gene ID" value="MESCA008845"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860243_0_0_1"/>
    <dbReference type="OMA" id="ICANIMA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF33">
        <property type="entry name" value="AT22075P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="7"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008845-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="14799" checksum="28726372CE20FAB4" modified="2013-10-16" version="1">MEGSVKNQYLATICANIMALSYGAFCGWPSAAFLVLQSDESPMDKGPISNEEASWIGAIICAGGILGNFFFGWLSNKIGRKKALMFSATPMAVAFLMIPFSKSVLHLCMSRFIGGFAGGGAFAVIPIYITEIAEDRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYV6</accession>
    <name>T1GYV6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYV6"/>
    <dbReference type="STRING" id="36166.T1GYV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009043-RA">
        <property type="protein sequence ID" value="MESCA009043-PA"/>
        <property type="gene ID" value="MESCA009043"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1464807_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009043-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="19938" checksum="D3942456F3399620" modified="2013-10-16" version="1">MPAIVIDGGTNEDSSRLNSTVNGVINNIFSSTNLDFSLSPPSVRNLVASVNVLYQQNDEGGSSSSTGNSDSNFSDSLDNFVDVDDDLFGSPMPFDASENGLWNGRFVPPPPRPPFLMDDPIISEGISSTCDLCSWAMPTKSTFIFEGTIEKASELGWPLTLIIVSVLSAILGAILMVIVVRCRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYW9</accession>
    <name>T1GYW9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYW9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009056-RA">
        <property type="protein sequence ID" value="MESCA009056-PA"/>
        <property type="gene ID" value="MESCA009056"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515212_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009056-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9314" checksum="64FE03E5B9CCF4D9" modified="2013-10-16" version="1">MSQLCVVELARLADDIAELLICKIPVWITTVSNDSPSNLGTFVVCRSNSRVQIYEVLHNLDVMIAYATLISSCVRSEGVQRAACV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYX4</accession>
    <name>T1GYX4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02036655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009061-RA">
        <property type="protein sequence ID" value="MESCA009061-PA"/>
        <property type="gene ID" value="MESCA009061"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690671_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009061-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8838" checksum="FF2E2C0D9D5AE799" modified="2013-10-16" version="1">MRGADTRGSPEKRSNIGWRDVECDRAVADKEESYIQRYNRDEKRDIKRKGGKLLNFISKREENLSKWKSKTSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYX9</accession>
    <name>T1GYX9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02021119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYX9"/>
    <dbReference type="STRING" id="36166.T1GYX9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009067-RA">
        <property type="protein sequence ID" value="MESCA009067-PA"/>
        <property type="gene ID" value="MESCA009067"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158996_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12354">
        <property type="entry name" value="RRM3_TIA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000317">
        <property type="entry name" value="Rox8, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="28"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009067-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12322" checksum="71ED56A0E43F0FF0" modified="2013-10-16" version="1">KPPSTKPGENVKPLTFDEVYNQSSPSNCTVYVGGVNSALTSLTEEILQKTFSPFGSIQEIRVFKDKGYAFVRFSTKEAATHAIVGIHNTEINSQPVKCSWGKESGDPNNVQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYY2</accession>
    <name>T1GYY2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cation-transporting ATPase</fullName>
            <ecNumber evidence="1">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02143479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYY2"/>
    <dbReference type="STRING" id="36166.T1GYY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009070-RA">
        <property type="protein sequence ID" value="MESCA009070-PA"/>
        <property type="gene ID" value="MESCA009070"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2090801_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="2">
        <location>
            <begin position="36"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009070-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13816" checksum="6B28D8DD2979C5BE" modified="2013-10-16" version="1">MKMVNLNGDLKKQNEYLDQLLRAPKTTSILHQGSPEELEITGYVRSKWKTFFTWIGITLTLGILRLVLYWYPHWLLLATHSPSALEQAEKLLVRQNFQGNHTIYYVKTINTLDWTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYZ5</accession>
    <name>T1GYZ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YPP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02183076">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYZ5"/>
    <dbReference type="STRING" id="36166.T1GYZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009083-RA">
        <property type="protein sequence ID" value="MESCA009083-PA"/>
        <property type="gene ID" value="MESCA009083"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779561_0_0_1"/>
    <dbReference type="OMA" id="EVYYTLA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="16673" checksum="72FBECEC8A04CC29" modified="2013-10-16" version="1">MDEPHSALKTLQTLSEAATFMSSFSPKSGPQRAWMLQLKIWLLLADVYLAVDQPEEASNCINEASQIYPLAYQNMLTRGQYFVHIGKLEDVKENFFNAVAANPYNTESLRALGEVYYTLAEKFTRDAAKYNPDCYKSCNRQRQNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZD9</accession>
    <name>T1GZD9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009238-RA">
        <property type="protein sequence ID" value="MESCA009238-PA"/>
        <property type="gene ID" value="MESCA009238"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742932_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009238-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8531" checksum="9BA38A6189270E47" modified="2013-10-16" version="1">MLSHKISTWCEITVEYGWGKAKYLVNYLCNLHGFSPRRCNIVQLFQHLALNECYQHQQHYLHDIEPDIGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZF3</accession>
    <name>T1GZF3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02382180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZF3"/>
    <dbReference type="STRING" id="36166.T1GZF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009252-RA">
        <property type="protein sequence ID" value="MESCA009252-PA"/>
        <property type="gene ID" value="MESCA009252"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253087_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009252-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11778" checksum="52A3DC9DC06E3B9F" modified="2013-10-16" version="1">MLCPAYAIQKQKSNFTKFKKQKLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDTHPIPNPKSKKQKSLADAIKQIASSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZL8</accession>
    <name>T1GZL8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-dimethyl-L-lysine(36) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ROX family. NO66 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02121789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZL8"/>
    <dbReference type="STRING" id="36166.T1GZL8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009322-RA">
        <property type="protein sequence ID" value="MESCA009322-PA"/>
        <property type="gene ID" value="MESCA009322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976053_0_0_1"/>
    <dbReference type="OMA" id="LRANICR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.40:FF:000001">
        <property type="entry name" value="ribosomal oxygenase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049043">
        <property type="entry name" value="WHD_RIOX1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21233">
        <property type="entry name" value="WHD_RIOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RIOX1/NO66-like C-terminal winged helix" evidence="14">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14933" checksum="94D49BC4BEF897EF" modified="2013-10-16" version="1">IEKSEEQKTVFGVKNELDATGNLVEPTYISSESNVRLLRANICRLVREEGTYRLYFYVDNSKEYHEYEVNFIEVEEEDVKCIKKLIQSYPNYLKVENLPHSEDEYKVAIVESLWSRGLLCCEKNVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZZ2</accession>
    <name>T1GZZ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02383527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02383528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009454-RA">
        <property type="protein sequence ID" value="MESCA009454-PA"/>
        <property type="gene ID" value="MESCA009454"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2981418_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009454-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="58" mass="6872" checksum="72714B57C40F50CD" modified="2013-10-16" version="1">MSSQEEKVEKLLNLADEVINIREQFPKMYEQILRKKTILEIQQMISLTSLLGSHERKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1H064</accession>
    <name>T1H064_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable nuclear hormone receptor HR38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear receptor subfamily 4 group A member 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Forms a heterodimer with USP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02168773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H064"/>
    <dbReference type="STRING" id="36166.T1H064"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009532-RA">
        <property type="protein sequence ID" value="MESCA009532-PA"/>
        <property type="gene ID" value="MESCA009532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451123_0_0_1"/>
    <dbReference type="OMA" id="VHAKRIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035259">
        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071376">
        <property type="term" value="P:cellular response to corticotropin-releasing hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06969">
        <property type="entry name" value="NR_DBD_NGFI-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000009">
        <property type="entry name" value="nuclear receptor subfamily 4 group A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003070">
        <property type="entry name" value="NR4A1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085:SF4">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01284">
        <property type="entry name" value="NUCLEARECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="32"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="20207" checksum="79C9CC2A2AB0E8EF" modified="2013-10-16" version="1">MSSAPSSAGSSPASAASSNNNSTIHLSHIPPSQLCAVCGDTAACQHYGVRTCEGCKGFFKRTVQKGSKYVCLADKSCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKSPQESPPSPPVSLITALVRAHVDTSPSDASCLDYSLYSDFTIHGQTVTEAETIQQFYNILTSSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0E3</accession>
    <name>T1H0E3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0E3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009618-RA">
        <property type="protein sequence ID" value="MESCA009618-PA"/>
        <property type="gene ID" value="MESCA009618"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064122_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009618-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13619" checksum="FBA69D19A8594391" modified="2013-10-16" version="1">MTVVILVLRNNGLDRSSLIRLKRHRFAVASFKIQRPYYCLCVFPHPLFTLALLREISRKSTLIGNGSYKALHYPTPLDPPGSCTKAMSLFENTPEENTLRENQLDNRISSDDIGEIERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0U0</accession>
    <name>T1H0U0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0U0"/>
    <dbReference type="STRING" id="36166.T1H0U0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009781-RA">
        <property type="protein sequence ID" value="MESCA009781-PA"/>
        <property type="gene ID" value="MESCA009781"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588657" evidence="3">
        <location>
            <begin position="17"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009781-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11372" checksum="C96BA839FDD7FF22" modified="2013-10-16" version="1">MKLAILFAALFAYALSTPLGEDAQILKFDNVHNGDGSYSFNVETSDGAARQEQGYLENAGTEHETLKVQGYYQFYGDDGQLYQVQYIADENGFQPQGAHLPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H138</accession>
    <name>T1H138_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucosyltransferase 24 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + UDP-alpha-D-glucose = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61304"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:59080"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02054121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H138"/>
    <dbReference type="STRING" id="36166.T1H138"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009890-RA">
        <property type="protein sequence ID" value="MESCA009890-PA"/>
        <property type="gene ID" value="MESCA009890"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1055893_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003980">
        <property type="term" value="F:UDP-glucose:glycoprotein glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040497">
        <property type="entry name" value="Glyco_transf_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009448">
        <property type="entry name" value="UDP-g_GGtrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226">
        <property type="entry name" value="UDP-GLUCOSE GLYCOPROTEIN:GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226:SF0">
        <property type="entry name" value="UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18404">
        <property type="entry name" value="Glyco_transf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06427">
        <property type="entry name" value="UDP-g_GGTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosyltransferase 24 catalytic" evidence="4">
        <location>
            <begin position="135"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009890-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="264" mass="30018" checksum="0B095FABDB46C8E7" modified="2013-10-16" version="1">GHCFDAQSGAPPRGLQFTLGTKENPLVVDTIVMANLGYFQLKANPGVWELRLRQGKSADIYDVTSADGLNTIHGKNSTRILISSLRSHVIKIKVAKKPDKMNADLLSDDEDNSGGIWNSITSSFGGSSEDEGETINIFSVASGHLYERLLRIMMVSTMKHTKSPVKFWFLKNYLSPQFTDFLPQMSKEYGFKYELVQYKWPRWLHQQTEKQRIIWGYKILFLDVLFPLDVKKIIFVDADAIVRADLKELMELNLNGAPYAYTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1I6</accession>
    <name>T1H1I6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H1I6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010048-RA">
        <property type="protein sequence ID" value="MESCA010048-PA"/>
        <property type="gene ID" value="MESCA010048"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1092255_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039303">
        <property type="entry name" value="CCDC50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22115">
        <property type="entry name" value="C3ORF6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22115:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010048-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="29243" checksum="448E2E1D603C8D29" modified="2013-10-16" version="1">MKISNLLSKSFRRRSMICSWETVTTRNGTTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPIEEIKEISNKMAQEKKDEAFARQLQELEKGGVAVSQEEKDRMLAIEAQDKELAKMLQDREKAKARRAKEKARLKKQQQKSLNESQISQEESPVIEDKRNESVHDVDLEPYSNPIDLIPHNFQYRNQHPNEKPRNLQHLAYGKDDDLYTLPFDTRNGGHIPVANPVVNVVQNQNNRPNSMHVSTEGSVHMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Q4</accession>
    <name>T1H1Q4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02386822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Q4"/>
    <dbReference type="STRING" id="36166.T1H1Q4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010121-RA">
        <property type="protein sequence ID" value="MESCA010121-PA"/>
        <property type="gene ID" value="MESCA010121"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_0_4_1"/>
    <dbReference type="OMA" id="HMHASAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010121-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="17536" checksum="FF8B8C2C3D49CDE5" modified="2013-10-16" version="1">MHAFALFNLSEFLVPGAHILDVGSGSGYLTACLARFVKNKGDHPDTKIVGIEHQPNLVALSKENLNKDDESLLSSGHVVIVQGDGRKGYPELGPYDAIHVGAASPETPTALIEQLKKGGRLLVPVGPEGGTQYMEQFDKDDRGNVQRTRLMGVMYVPLTDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H229</accession>
    <name>T1H229_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Atos-like conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATOS family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02384314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H229"/>
    <dbReference type="STRING" id="36166.T1H229"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010260-RA">
        <property type="protein sequence ID" value="MESCA010260-PA"/>
        <property type="gene ID" value="MESCA010260"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_524088_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033473">
        <property type="entry name" value="Atos-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025261">
        <property type="entry name" value="Atos-like_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051506">
        <property type="entry name" value="ATOS_Transcription_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199">
        <property type="entry name" value="GH03947P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199:SF11">
        <property type="entry name" value="PROTEIN ATOSSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13889">
        <property type="entry name" value="Chromosome_seg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13915">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01177">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atos-like conserved" evidence="2">
        <location>
            <begin position="400"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010260-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="520" mass="59066" checksum="6D2F5EDB1857F738" modified="2013-10-16" version="1">MHSIPVLNLNKQPFLYSLSSIKVTKADDENPDSSQSEDFYEKKIYGDENEDFQNIMRYKRLIKKRDICKRKSETLDCSEQRQLKMPELGSINSIRPPLYQTISSTISEAVQTSCVEMISVGTQTVYNEEENFCDNCGSTKPNLCLNCFDISKKEFSTLNRLRKSADKSKDHIDKAELLLSAIQRTPNNKKKCLKDNRGHRLSPSQQDCREKGDCILCKRQKTQKLHLNSLFNNNSFSNYNEFVTIENCQHSTEDSENGFNTNSYFYPSPNTQFKTPKNQITLSPAKTNRSKCNSDTKKNTDLNYENNFVSHNKKILPKVNLTELFCNPGMNLNLLSVCSSLPSKISSFDYSCEKPVQKSNSAPSLPSTSSPTCLSPRFIKQSITYQFRRSRHLSEDPTDLLLEESILQKRISPSKYVSGFKMLLGASGGFCPPQMTIPAVCYFYEINGDTINTPFQTSKSGRLTLHTDVRAIISRKVDCDTAAAHAKGDLEPKELKILTVIPENPRYSTRLENSNKRYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2L5</accession>
    <name>T1H2L5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2L5"/>
    <dbReference type="STRING" id="36166.T1H2L5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010465-RA">
        <property type="protein sequence ID" value="MESCA010465-PA"/>
        <property type="gene ID" value="MESCA010465"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239659_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010465-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11972" checksum="ED0BB7F47E28F75A" modified="2013-10-16" version="1">MVWYQERNMFKSVTLGFQYKMRVYMLISPLTAFLLNKTQLLKSVTSWERNTSVEMVKGVTVVNSTAQKDNLIIEGNDIEAISGSAALIQQSTNKMMKKSLFGDFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3T6</accession>
    <name>T1H3T6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3T6"/>
    <dbReference type="STRING" id="36166.T1H3T6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010923-RA">
        <property type="protein sequence ID" value="MESCA010923-PA"/>
        <property type="gene ID" value="MESCA010923"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_606334_0_0_1"/>
    <dbReference type="OMA" id="HTGLHEF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF167">
        <property type="entry name" value="ARRESTIN C-TERMINAL-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="138"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010923-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="452" mass="51644" checksum="1933242B68C5E2C9" modified="2013-10-16" version="1">NSYSNKWQREVNWNVRKTRRVNGKTEYYTVRYSSHDTYLASRTYVLGQHGGPSFELQPGTHQFTYTVDLPHGVPTSFVGQYGEIKYEVLVTIDRSMRFDNVFKKPFTVVRTMNLNMIPTLGIPIEREAESSFCCCCCASGPINMKLSLPFSGFASGQKIPYNIIIDNEAGSDVNNVEVKLIQRIVFLSRSPERAQKVDKFTLYSENLGSVLKYTKKEFNKEIQLGSLPPSNEDPGDIIQISYYLMAKMKKGFFHTNEDVKIPITIGTVPLIQSSRNPNNVISMQPTAPLLTDDDMGGDKPPPYFTMEFLGNAKRKPKVFYEVNPGTQLLTKHLLDDKDFCEKLVLVENIEDFVPKAEELHSLYPEKVKVYHKDFVNIWKLVYQDKLDNGSRVPDLLIDMPKQPYEEEPSTAIYAGVGSYNFLKHIINSIVFQTSFLSYGRCDMFFVIPPPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H413</accession>
    <name>T1H413_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388590">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H413"/>
    <dbReference type="STRING" id="36166.T1H413"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011011-RA">
        <property type="protein sequence ID" value="MESCA011011-PA"/>
        <property type="gene ID" value="MESCA011011"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078126_0_0_1"/>
    <dbReference type="OMA" id="KPQHGTR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MIR" evidence="3">
        <location>
            <begin position="4"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="3">
        <location>
            <begin position="66"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011011-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="21737" checksum="A36AF22A5142899B" modified="2013-10-16" version="1">SKTPRVVTYGSILKLLNQDYGYRLHSHDVKYGSGSGQQSTTGIELKEDVNSHWVVKTTEDKFVNRGEHVKCGDVIRLEHLTTKKNLHSHMFSSPLSGEQEISCYGNDGEGDSGDNWQVVCGGESWNRDLPVRFKHKDTSAYLGMSGRTFGRPISGQMEVVGYSSAQHGTKWTTAEGLFISPSEEEHLDDYAHTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4L5</accession>
    <name>T1H4L5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02083969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4L5"/>
    <dbReference type="STRING" id="36166.T1H4L5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011227-RA">
        <property type="protein sequence ID" value="MESCA011227-PA"/>
        <property type="gene ID" value="MESCA011227"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_0_1_1"/>
    <dbReference type="OMA" id="AMNILME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="70"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011227-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="327" mass="36240" checksum="1D98496077DC140E" modified="2013-10-16" version="1">MSSPNEKSSIAIEFRPNNYGTNENCTSNCNNNDVIGGTLYCLHGQNNIEVRGHCHKNRIEGVDIKARKKLIIASVLCVIFMIAEIIGGIMSNSLAIATDAAHLLTDFASFMISLFAIWVAGRPSTKRLSFGYWRAEVIGALASVVMIWFITAILIWLAVERIINKTYEVDAKIMLITSGLAILVNVIMGIQLGHGHSHGGSSSPQDGSHSHDVNINVRAHLSTILKDALMVLMEGTPSYLQYTEVLQIFEQIEGVERVHNLRIWALSLNKVALSVHLAIRSDTNPKHILDQATSAVHVKYHFYETTIQIEDYIPEMEDCQQCSGPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4R0</accession>
    <name>T1H4R0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4R0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011277-RA">
        <property type="protein sequence ID" value="MESCA011277-PA"/>
        <property type="gene ID" value="MESCA011277"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592482_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577362" evidence="1">
        <location>
            <begin position="19"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011277-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9728" checksum="509C149D05707058" modified="2013-10-16" version="1">MKYIIFLIFICILPIFESAPTDQKCLLKDKDTNFCLKMANYPRKQIEKLRNIPYKRLREYKLLQPKIYDTRILVWSSKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4S3</accession>
    <name>T1H4S3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02049163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4S3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011291-RA">
        <property type="protein sequence ID" value="MESCA011291-PA"/>
        <property type="gene ID" value="MESCA011291"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009655_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011291-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13124" checksum="77C5358E266374C3" modified="2013-10-16" version="1">MDWIRKVGLQNLVIDMNIVGGKTNVVGDVFDVTNVGVVGVGDVGVVGGVDVYDVGNYNVDVNTVVDDVYGNVYNKGHVVGDLVRVFDVVGVNVVRVIETCKNGSLYVVGVQGVKTVEQQGVVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H500</accession>
    <name>T1H500_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02377279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H500"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011373-RA">
        <property type="protein sequence ID" value="MESCA011373-PA"/>
        <property type="gene ID" value="MESCA011373"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266794_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011373-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11819" checksum="B47DFFDE2D8F0CC8" modified="2013-10-16" version="1">MCLNRLSVCILTALDPLQQQNSFKRRIQLMIQGALKDFDVQHMMDGELIITMNCTNSKTSRLDKNQSKTFNMCMSCGNNTVKYTIFFSNNLAHERGRSCPHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H547</accession>
    <name>T1H547_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02377583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H547"/>
    <dbReference type="STRING" id="36166.T1H547"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011422-RA">
        <property type="protein sequence ID" value="MESCA011422-PA"/>
        <property type="gene ID" value="MESCA011422"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1614987_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011422-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="17935" checksum="6FA4B38908687E25" modified="2013-10-16" version="1">MAVDVALRPIKPAKKVDISKLSLNRNKKEEMKTIKLGPIDEALKAIQQPKKVDSPKFVAPRPTKKDDEPVIRLAPLENILKPEVKAKKSPSPPLPGPLFAPPPIKGQIAPPGSTTPHPNLGNRTPGAKRYQPILPKTPRNNPFANIPNDVNRILQGAGTEIKSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5I1</accession>
    <name>T1H5I1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02375442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5I1"/>
    <dbReference type="STRING" id="36166.T1H5I1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011558-RA">
        <property type="protein sequence ID" value="MESCA011558-PA"/>
        <property type="gene ID" value="MESCA011558"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_317755_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="809"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="877"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="9">
        <location>
            <begin position="319"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011558-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="917" mass="99471" checksum="D001D066CB258B9C" modified="2013-10-16" version="1">RILTALCSRLEKGAATCAVEPYTLGENGSSPEKRQQEFTVSLLRHPVVAELVNRCGKRRAKNNREKMRLSPLAGVVLLIKVLFLCAFNLVHHHEREKPMGRVDGKVAIVSGAALGIGKAAALLLAQEGASVIVGDINKEEGQKTVSEIEGKGGKAFFVLLDVRKEEDWKSVIEGALSRFGRLESCTTLEDWRRVQSINLDGVFLGTKYAIEGIRQHGAGGSIINLSSIEGLIGDPELAAYNASKGGVRIFTKSSALHCAKAGYKIRVNSVHPGYIWTPMVAGLTQEDEAARKRLVDLHPIGHLGEPDDIAYGILYLASDESKFVTGSELGNRMSTLPNGLTDAEARKRLREEGPNDLARSGNRSRWRVVLGVLKEPLFLMLLGAGGIYIALGDWHEALLLVSLGSASVFITVVQEVRSERVLDALRDLTSPRVLVVRDGTQQRIAGKDVVRGDWIVLGEGDLWLRMLWYDLKTRVLTRRAAAIESLGAATVLCTDKTGTLTENRMTVTEIQTDAGGMIDVRSSDLSSLDGTVTRIIHVGRYACQPDPFDPMEKAFHEISRHEQGQEKPLTVHLVRDYPFGDEVLAMANVWKLENGRLLVASKGAPEAVMELSHLGSDARAAWRRKVDEMAARRKASAGSTSGFSFTFLGLVGLTDPLRANVRDAIAECKTAGIRVVMITGDYPLTAQAIAREAGIRAEKVLTGADLEDTSGLPLSDRVKDIDVFARIRPAQKLEIVQALQSNGDVVAMTGDGVNDAPALKAAQIGIAMGQRGSDVAREASAIVLLNDDFSSIVKTIRLGRRIYDNLHKAMGFILAAHVPIAGLAIIPLFSGLPLILLPAHIAFLEIVVDPICSIAFEAEPEEADLMVRPPRDSHRPLFSGSLMLWSGVQGGIVLIALCLVLFMGAWRGMAEETLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5I5</accession>
    <name>T1H5I5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374539">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5I5"/>
    <dbReference type="STRING" id="36166.T1H5I5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011563-RA">
        <property type="protein sequence ID" value="MESCA011563-PA"/>
        <property type="gene ID" value="MESCA011563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_4_1"/>
    <dbReference type="OMA" id="RYLQWNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000034">
        <property type="entry name" value="Eupolytin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004578014" description="Peptidase S1 domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="25"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="25981" checksum="5C27460E9210A56E" modified="2013-10-16" version="1">MRNILLSLTIFSLLGFSLAIPNGRVVGGSDAEKGQFPHQVSLLKNNAHTCGGSIISTKHILTAAHCVVMGDGVQPYPSRFFMVRVGSINRLAGGELYDVQQVIVNQKYGNFLNDVAIVVLAKPLTLSPYVSIIELADEEVPAGETVRISGWGRLTQGGILPVKLQYNDLKAITKEECKNRIKWNEDSLLCLDHPKDNGACNGDSGGPATYKGKVVGIAGFVYGGCGSSNPDGYARSIITNNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H676</accession>
    <name>T1H676_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA polymerase III Rpc82 C -terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H676"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012209-RA">
        <property type="protein sequence ID" value="MESCA012209-PA"/>
        <property type="gene ID" value="MESCA012209"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982038_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012209-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="284" mass="32569" checksum="55256A7111295946" modified="2013-10-16" version="1">VMISAIEKKLGSSAGECFKYILKTMYTKTHPWQQSYSNPFTMAEVKHLIDKSSKNLELMKYLDQYMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFEKNENKRLIDKSQKLESIVDAMKERGDAEEFIDEIRETMTPPEREILDKVKTRLKTLANAELALDETIFLFQMYLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H695</accession>
    <name>T1H695_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydropteridine reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H695"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012228-RA">
        <property type="protein sequence ID" value="MESCA012228-PA"/>
        <property type="gene ID" value="MESCA012228"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357669_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004155">
        <property type="term" value="F:6,7-dihydropteridine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070404">
        <property type="term" value="F:NADH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15104">
        <property type="entry name" value="DIHYDROPTERIDINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15104:SF0">
        <property type="entry name" value="DIHYDROPTERIDINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="21841" checksum="8CC52688579F0D01" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGWTSSISATVGAHFLKEGGMLAITGAKPALEVTTVMIGYGMARAAVHQLTKSLSGSNSGLPKDSLSVCILPITLDTPMNRKMPSADFTTWTPLSFIAYFIRTNGEGHPKNGSLLQLVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6C7</accession>
    <name>T1H6C7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 UFM1-protein ligase 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">E3 UFM1-protein transferase 1 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">E3 UFM1-protein ligase that mediates ufmylation of target proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UFL1 family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6C7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012260-RA">
        <property type="protein sequence ID" value="MESCA012260-PA"/>
        <property type="gene ID" value="MESCA012260"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_571860_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061666">
        <property type="term" value="F:UFM1 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018611">
        <property type="entry name" value="Ufl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056580">
        <property type="entry name" value="Ufl1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056579">
        <property type="entry name" value="Ufl1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057:SF0">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09743">
        <property type="entry name" value="E3_UFM1_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23659">
        <property type="entry name" value="UFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25870">
        <property type="entry name" value="WHD_UFL1_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like" evidence="8">
        <location>
            <begin position="433"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012260-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="478" mass="54164" checksum="1074B8FCE45AFBDC" modified="2013-10-16" version="1">INDIAEKISKDDPQIHFMLGQLINEDYIISIAQEINEKLQQTGEINVSDLTVQFDLPSDFIQRTVMEKNLNKIIKGRQDQSNQRVFFTQAYITRCKCKIRGALAALTRPTNISYLIQQINVPEKLFHTIVDEINPAGTVTSKQTNALYIPHIYAKMQSDWVNSFFKQNGFLEYDAISKLGISDPKSFIKKMFPNEDMTFLERCAVGSKIIDLTLLTHMSTKSYVDLATILPSNLSEEDIEELFQTILNTKNIPNNFVYLETTAFSKTYLQELVAPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKLFEATPQNNRRQTHASPFKYLLKSLGNDICNELTIYVAGECNLQLKNADNLNVDQRNKIAQEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6H1</accession>
    <name>T1H6H1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6H1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012304-RA">
        <property type="protein sequence ID" value="MESCA012304-PA"/>
        <property type="gene ID" value="MESCA012304"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968297_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012304-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="32067" checksum="9BA666A2D2A5EB45" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSIFDIFADCHEPNDSVTQAGGVQRLSMLLQSPKQNIVKEAAWSIIRNVLEKGDFKSQKEAAYNTTTAGTLEKIVDLIEKYQILTKTLGGTDILCLMVEEMGGLDKLESLQKHENEEIYKKAFAIIDTYFSERNDAQNEPAPKKVNRALQFGASQAPEGGFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6H8</accession>
    <name>T1H6H8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6H8"/>
    <dbReference type="STRING" id="36166.T1H6H8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012311-RA">
        <property type="protein sequence ID" value="MESCA012311-PA"/>
        <property type="gene ID" value="MESCA012311"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025162_0_0_1"/>
    <dbReference type="OMA" id="RVESANC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20872">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20872:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012311-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="31605" checksum="918FDC01E4F3141D" modified="2013-10-16" version="1">VCKHWTDVFYSPYVWNNFIVDDRTLTRTKYNYYSGWQHTIDHARTHTCLSRVGRNLFRPLHSFNNIFQFMTILSWCIDRSMETENKDIKTSLSYDFPCTMSQYDDRENLKLFGTGGEMLRTLKILLSKLAKLKTLKLTDLVLERFEANRLLDEIAENCHFTLNHLNIVNVTVVHCPILHIGCFFNLQNIAPNIDDDVITLIADSKITQLHLVQNKHTPTAISLSPCSAKAWRIVKRDNPNLKVHLRVESANCGEFVFQPEAPIYSILYRAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Q0</accession>
    <name>T1H6Q0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6Q0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012383-RA">
        <property type="protein sequence ID" value="MESCA012383-PA"/>
        <property type="gene ID" value="MESCA012383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003782_4_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="506" mass="57442" checksum="149ECB781F2A2AE9" modified="2013-10-16" version="1">DFINDMQVMCNMIADGPLKSFCYRRLSYLSSKFNLHVLLNELRELANQKAVPHRDFYNIRKVDTHIHAASCMNQKHLLRFIKKTLKNNSDEIVTVTKGQQMTLTQVFQSMNLTAYDLTVDMLDVHADRNTFHRFDKFNAKYNPIGESRLREVFLKTDNFLNGKYFAQIINXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPLMEEYSIAAQVWKLSSCDMCELARNSVIMSGFPHKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6X5</accession>
    <name>T1H6X5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6X5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012457-RA">
        <property type="protein sequence ID" value="MESCA012457-PA"/>
        <property type="gene ID" value="MESCA012457"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093981_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012457-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="17204" checksum="C4ED319D055B7123" modified="2013-10-16" version="1">MTDNASKFLGGVLGDISSRSAYTQILLGAASGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADRFIDRKLDKAESLLNKGERKAKKWYSRLIGDENGPRINDLHIFVTAFVGGVVLGVACA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7L9</accession>
    <name>T1H7L9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein heavy chain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7L9"/>
    <dbReference type="STRING" id="36166.T1H7L9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012756-RA">
        <property type="protein sequence ID" value="MESCA012756-PA"/>
        <property type="gene ID" value="MESCA012756"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922339_0_0_1"/>
    <dbReference type="OMA" id="NCEVTRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF20">
        <property type="entry name" value="LOW QUALITY PROTEIN: DYNEIN BETA CHAIN, CILIARY-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012756-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="15519" checksum="E4EA4A35E5AFAE1E" modified="2013-10-16" version="1">VALQECERMNILIKEIKRSVRELLLGLKGELTPVMEKLMLCYDHIPDTWMRLAYPSLMGLQSWFSDLILRCKELGYWLSDFKLPSAIWLGGLFNPQSFLTAIMQETARKNEWPLDRMCLNCEVTRYEKDDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Y2</accession>
    <name>T1G9Y2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069264">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02069265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Y2"/>
    <dbReference type="STRING" id="36166.T1G9Y2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000024-RA">
        <property type="protein sequence ID" value="MESCA000024-PA"/>
        <property type="gene ID" value="MESCA000024"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1620929_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055121">
        <property type="entry name" value="HTH_69"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705">
        <property type="entry name" value="AGAP000321-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705:SF1">
        <property type="entry name" value="PNP_UDP_1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22979">
        <property type="entry name" value="HTH_69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn-helix" evidence="1">
        <location>
            <begin position="35"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000024-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="18830" checksum="695806E3480DDB1E" modified="2013-10-16" version="1">MFVVSPLFLSLFHLNLNNSIKFYLSTVSIDESQLESPPLTQEKVLELIEQAYPNPITAEDLAKDYGWEEADVTLVIQSLISRGLIKSMEYNSYTRIHHEDKEIKVVKQMPTISSNKQPTIAIVTAQYCEKMAIDAMLENKETFVRYTTVVIMKTVNNQYITFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAC7</accession>
    <name>T1GAC7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAC7"/>
    <dbReference type="STRING" id="36166.T1GAC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000182-RA">
        <property type="protein sequence ID" value="MESCA000182-PA"/>
        <property type="gene ID" value="MESCA000182"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796894_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000182-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7669" checksum="9AA3EF206E59E2D4" modified="2013-10-16" version="1">MLANETWTIGDVQAVLLAMLDSKTMLIVHSLNSDMKALKLIYGCIVDTLILYPHEMGTIYETSFENTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB27</accession>
    <name>T1GB27_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000448-RA">
        <property type="protein sequence ID" value="MESCA000448-PA"/>
        <property type="gene ID" value="MESCA000448"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504280_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000448-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9824" checksum="7B6ED8FEEDB22EF4" modified="2013-10-16" version="1">TITHILTSNFNECITKGEFPDCLKETVITPNFKEGDKILHQLNFCLDKGQYVVLLFIDLRKAFDLVEHTSMLEILDKLGIRGVSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBB1</accession>
    <name>T1GBB1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBB1"/>
    <dbReference type="STRING" id="36166.T1GBB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000538-RA">
        <property type="protein sequence ID" value="MESCA000538-PA"/>
        <property type="gene ID" value="MESCA000538"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_736286_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000538-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="376" mass="42458" checksum="C2D9E3EC40D25827" modified="2013-10-16" version="1">MFEKHDKQLGISNPNKSMYCNDLGCQEEIGQLGEALAIEAEKALGTLLCFSTDKTLRSVFIQGCLQNLATNKSVIVSLRLLPKLFASFQQFRTGGTYEITLWAERQHKMMFHFFNNIKYYASIYKNSEKLVLNANGQPMYSHMTQVSVRLQFLSSVYSSLGSPKPFRLSLDQVDALWEWLAHDPECADCLFAWLQCQAKGIDQHALDINAEMADVLWGDSHSDGEAINDSDDQVHNSVMKHLPVEGPYVRLLTNSAITDFLNDQDNDNGSYSSPMSNKSEKNMADFDDDDSPCEEEIAQLVESSGIIPKYNAFTALREKQQQERKEKELEECIGKIDPNGLPVFRVSEVCQPGKTLLWDLLQDNKIVSTLNIIECN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2021-06-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBC6</accession>
    <name>T1GBC6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA000557-RA">
        <property type="protein sequence ID" value="MESCA000557-PA"/>
        <property type="gene ID" value="MESCA000557"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3435125_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000557-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="14" mass="1638" checksum="7EDE55E26A0694DB" modified="2013-10-16" version="1">MHNLIKEATLVQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBJ1</accession>
    <name>T1GBJ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02068464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBJ1"/>
    <dbReference type="STRING" id="36166.T1GBJ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000623-RA">
        <property type="protein sequence ID" value="MESCA000623-PA"/>
        <property type="gene ID" value="MESCA000623"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2114984_0_0_1"/>
    <dbReference type="OMA" id="KTIVGEX"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000623-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="115" mass="12675" checksum="5045AE4D89DB3D2C" modified="2013-10-16" version="1">VVANTCGPYRFFGEQVVQACIKSGAHYVDISGEPKFIETMQFKYNKEAEEKGVYIISACGLDSIPSDMGTVFVEKNFNGVVNSIETYIESFYTGEDQNGGGLINFGTYESLVEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBM4</accession>
    <name>T1GBM4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBM4"/>
    <dbReference type="STRING" id="36166.T1GBM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000660-RA">
        <property type="protein sequence ID" value="MESCA000660-PA"/>
        <property type="gene ID" value="MESCA000660"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076720_0_0_1"/>
    <dbReference type="OMA" id="PETDEMG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF4">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="47"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000660-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="302" mass="34645" checksum="3BDA44ECC141083D" modified="2013-10-16" version="1">MEEEEEPLYQYVPETDEMGSQSLDGCLDDSFTQSLLLLNDGLASGALLTQYEQMYRKNPDLPITEARKSENIPKNRYRDISPYDCTRVVLVNSSSGDYINANYVNMEIPNGTVNRYIATQGPLATTTLDFWRMVQQESSHLVVMLTTVMEAGRQKCYQYWPVTGEELELTDDFSVKCLSEQADATGSFVFREFILSDTKTGENRSIQHMQYLAWPDHCVPSDPQQFLEFTEKVRTARNKTLLQEIEESLKQDSSGKELQLGVTSIGLLVFQNGIRVNTFSWAKMVKVSFKRKDFFIQLRREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBN8</accession>
    <name>T1GBN8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000674-RA">
        <property type="protein sequence ID" value="MESCA000674-PA"/>
        <property type="gene ID" value="MESCA000674"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815385_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000674-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7860" checksum="B3D2A80C3AC7B80E" modified="2013-10-16" version="1">MIYDPIRDDDVVKNIRSKCLSWVGHVVRTDDDRPSKKVFSKALHGQIIKWVHLVNKDTRDTQVERNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBV8</accession>
    <name>T1GBV8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02029643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBV8"/>
    <dbReference type="STRING" id="36166.T1GBV8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000747-RA">
        <property type="protein sequence ID" value="MESCA000747-PA"/>
        <property type="gene ID" value="MESCA000747"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_349606_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018123">
        <property type="entry name" value="WWE-dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00678">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="WWE" evidence="6">
        <location>
            <begin position="705"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="230"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="290"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000747-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="806" mass="88398" checksum="1DC8DAE9D0A2E92B" modified="2013-10-16" version="1">MAESVNSTTLSALTEGLNSTNSRKSSRQVCSSSATTLKSVESTAFSTTKSAATNSGIASTSANTPREDRKRKHLNVEPSTSYLSKTTPSKRKRSSHQFEIIEDCIAQRTRSKTVSPEDKKKQQPEVATTQKAKRNLLNYYRTTKNNHQNVIKPSGSSGNSSNKSSPIVNKKLQKTPNKKIQKTPLKKNNILNNKKSKSGKNKKIKSPSSKSNVVSKASTSKFYLKGLAENTKKEQSLRRSIRGKTGSCVSNSTAAANNSESGGVPNASSESPAAVASTSDRVENPFSKFSPGNLNVQDSNLMAQQSNNGTQNVNSNDQSLLGSNSTNTGATTTTTTTNSTGHGDSESDDSEVGRLQALLEARGLPPHLFNALGPRMSHILHRTMGNSTSNKAQQLLQGLQSADESQQLQAAIEMCQMLVMGNEDTLSGFPIKQVVPALIILLRMEHNFDIMNNACRALAYMLEALPRSSATVVDAVPVFLEKLQSIQCMDVAEQSLTALNILSRRHHKVILQANGISACLTYLDFFSINAQRAALTITANCCLNMNADEFHFIKDNLEMLARLLTNHDKKCLENICTAFYRLVESFQNDSAKLKLIASSELLKNCQQLLVITPTLLNTGTFSNIVRMLAVMCGSCPDLAVILLRNNIAATILYLLADSSSSLPGNDIVILNARSPGELYDITCLIAELMPNLPKNDIFSVDVLLERQEYGFNVQDQIQWQWKDDRGSWHSYSSMDSRLIETAHLNSDDEISLNTLGRTYTIDFHTMQQINEETGTTRNIQRKVNTNNMNMAISESDSNISDARIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCI3</accession>
    <name>T1GCI3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02036527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036529">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02036530">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCI3"/>
    <dbReference type="STRING" id="36166.T1GCI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000997-RA">
        <property type="protein sequence ID" value="MESCA000997-PA"/>
        <property type="gene ID" value="MESCA000997"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_77_0_1"/>
    <dbReference type="OMA" id="PECERPF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000373">
        <property type="entry name" value="Putative transcriptional repressor ctcf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000420">
        <property type="entry name" value="Putative transcriptional repressor ctcf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001668">
        <property type="entry name" value="transcriptional repressor CTCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000049">
        <property type="entry name" value="transcriptional repressor CTCF isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="220"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="372"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="569" mass="66110" checksum="C4A1F9DDCBCE0145" modified="2013-10-16" version="1">MSSQHIKDEENLQEFLETFEANETQEDEQEENGTYFVDEAGNYYYQATPDSEPVPTVKPEGMIEEDDDEEEEAQDEEEENVLIEKEEIYEFDETEDVEEQEIVDGDDVKPDMKMLRMSKTVIKPKEYVTEENGVFPCTHCNYVSNKRYLLARHMKSHSDDRPFKCSVCERGFKSQPALQNHINTHTGNKPHHCKFCDSKFTTSGELVRHVRYRHTLERPHKCTECDYASVELSKLKRHIRCHTAEEALKIHTGEKPYECDICHARFTQSNSLKSHKLIHSVADKPIFQCELCPTTCGRKTDLRIHIQKLHHSEEPIPCKKCNQQFSDRYSYKLHMKTHEGEKCYRCGLCSYASISQRHLDSHMLIHTDEKPFKCDKCDLSFRQKQLLKRHENVYHNKDYVAPEGKQKTQKCPHCQKSFAHKGNLIRHMSTHDPESNVHTDKEMLRLGRTVKIIPADKNTGNFEVIDESGEDGETVYFEDDEDMEEDEEEEEPDDAEEEYIDELIDVDNNKVKSEDLIEEDEDGEQYVVLEVMDDDEEGTTIDQSDGKTIVISADDSQLNLDGGTKISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCK6</accession>
    <name>T1GCK6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCK6"/>
    <dbReference type="STRING" id="36166.T1GCK6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001021-RA">
        <property type="protein sequence ID" value="MESCA001021-PA"/>
        <property type="gene ID" value="MESCA001021"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909066_0_0_1"/>
    <dbReference type="OMA" id="RNCEDDH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001021-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15102" checksum="D5512947D7AE4894" modified="2013-10-16" version="1">MDPQKQAISEDLYNLTQLAEVSLAVGPINTSFKTEQSQTPQKDSKILSSSDEDSVGYYSHKLFDRKKVRRSFNSESYSSGNGSQSSSGEENSYEPKMRRNCEDDHVCPECGKKYSTSSNLARHRQTHRCFTYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDJ5</accession>
    <name>T1GDJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02025498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDJ5"/>
    <dbReference type="STRING" id="36166.T1GDJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001383-RA">
        <property type="protein sequence ID" value="MESCA001383-PA"/>
        <property type="gene ID" value="MESCA001383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_2_1"/>
    <dbReference type="OMA" id="TAMWHIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000398">
        <property type="entry name" value="Monocarboxylate transporter 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF284">
        <property type="entry name" value="EG:103B4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="328"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="524" mass="56880" checksum="6FB4BADF56B3E176" modified="2013-10-16" version="1">MFVTEFLDYFKEGNGYTSMIVSLLVGVTLCSGPISSAFVNRYGCRPVTIAGTLLASTCIALSYFAQNILTLIFTIGIGAGFGFGLIYLPAIVSVTMYFEKYRSLATGIAVCGSGLGTFIFSPLSAYLIETYGWRGTMLIIGGITLNCIIFGATFKPLQSGPAKLPENTLSRSHSVGQQINGTYSNESINSKVPSIDSTAAPNGIRTAVSHQNITEDGNKLKSRSASGTMYRPDILYQGSLASIPDHATSRFDVRPKGGYGSLRRTNNGESQDFSDPIDEGFCQFYWPSETECEAETKKQCKILGFSCSPETRDTFDEMMDFELFKDWVFIIFVVSNFCTSIGFNVPYVYLPSVAKDYKLSITESSTLLGIIGIANTVGRIILGYFSDKAYVNRLLVYNICLTVCGISTVLSVFCVDFMSLAVYSAFFGFTIGAYVGLTSVILVDLLGLDKLTNAFGLLLLFQGIASLAGPPLAGWISDQTGSYTPGFLLAGIMIIISGVILFLIPPLQKYYAKRNKVIENNNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDV6</accession>
    <name>T1GDV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDV6"/>
    <dbReference type="STRING" id="36166.T1GDV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001507-RA">
        <property type="protein sequence ID" value="MESCA001507-PA"/>
        <property type="gene ID" value="MESCA001507"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186321_0_0_1"/>
    <dbReference type="OMA" id="NIECMDA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000002">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001507-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10289" checksum="CD4006C800B9C16E" modified="2013-10-16" version="1">MKVCILGDQQHCDEAKQNNIECMDAEALKKLNKNKKLIKKLAKSYDALLASESLFKEILLLLGSGLNKAGKFPGMLSYQESMTGKIDEVKATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDZ7</accession>
    <name>T1GDZ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA topoisomerase 2</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA topoisomerase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02072931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDZ7"/>
    <dbReference type="STRING" id="36166.T1GDZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001552-RA">
        <property type="protein sequence ID" value="MESCA001552-PA"/>
        <property type="gene ID" value="MESCA001552"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001935_1_1_1"/>
    <dbReference type="OMA" id="NHMDECK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="17">
        <location>
            <begin position="380"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="886"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="927"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="821"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="927"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="966"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="983"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1009"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1026"/>
            <end position="1038"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001552-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1076" mass="123332" checksum="E0578C5E5FD025FF" modified="2013-10-16" version="1">MENGSKAGGSKAIEKMYQKKSQLEHILLRPDTYIGSVEKHSESMWVWSNSEKKMMKRDITYVPGLYKIFDEILVNAADNKQRDKSMSTIKIDIDKEKNVISIWNNGEGIPVVMHKEEKMYVPTMIFGHLLTSSNYDDNEEKVTGGRNGYGAKLCNIFSTSFTVETSSRSYKKQFKQVWGSNMSKTSDPKIKDASSSDFTKITFSPDLSKFKMTELDDDIIDLMSRRAFDIAASTRGVSVFLNGTKLPIKNFKDYVDLYIKDRNDDSGNPLKIVYENYYKGLGTSTSAEAKEYFQDMDRHKIKFLYNGQDDDNNIEMAFSKKCIDQRKAWLTNHMDECKRRKNLGLAEKYLYKKDTKEINFSDFINLELVLFSNADNVRSIPCLIDGLKPGQRKVLYTCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLCGTIVNLAQNFVGSNNINLLRPEGQFDDAILNYEYDDNKKVEPTFYVPIIPMILVNGAEGIGTGWSTKIPNFNPRDIVENIRRLIVGEEPKPLHPFYKNFNGSIDFVSEHRYVTNGNIAILPNNRIEITELPIGTWTQNYKENVLEVLLNGNEKDKNKTTILSDYKEYHTDTTVRFVCTFNSGEFEKIYAEEGGFHRVFKLTSSMSTAQMHCFDNNNCLRRFDSANIILKEFFDVRMEYYVKRKEYLEGMLQAQADKLSDQARFILEKCNKTLVVENKQRKTMIDELIKRGYKPDPLKEWQRKIKVDEGEEVSENEEEEVVGASSIKKEKKKDLDPEAAFKKLTDVKKFDYCSKKEKKKDLDPEAAFKKLTDVKKFDYLLGMSMWMLTEERKNELLKQRDAKLLELKILKDKTTKDLYTEDLDEFMKALDDVEEKERLEESGQKKATAAAKKAMAKKAIKKRTSTADGSELTPDPKGVKVEFKVTGELIKKMEKAVHLAANKGEKKEKKEKKEKAEKEGGAEGDEFDNLVAGKKPAIKKEKKEPTEKKPRKKKEVSDGMKQSKLDFSKGKKKVNYSFNDESNEESDGELEMYDNDAVMEKSIKMGRLSEDDNDGDSGSASENSPVKKPAAKRVRKKVSSDSSEEEYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEJ4</accession>
    <name>T1GEJ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02057405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02057406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEJ4"/>
    <dbReference type="STRING" id="36166.T1GEJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001759-RA">
        <property type="protein sequence ID" value="MESCA001759-PA"/>
        <property type="gene ID" value="MESCA001759"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461104_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001759-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9781" checksum="109F31A0576542DC" modified="2013-10-16" version="1">MSVLEKLVKVDKKTAVVMAMDMLFAGIDTKFKENPSRNEDEMSVLEKLVKVDKKTAVVMAMDMLFAGIDTTSSAITGCLLNLAKNPQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEV6</accession>
    <name>T1GEV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02108211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02108212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEV6"/>
    <dbReference type="STRING" id="36166.T1GEV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001877-RA">
        <property type="protein sequence ID" value="MESCA001877-PA"/>
        <property type="gene ID" value="MESCA001877"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1113705_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006149">
        <property type="entry name" value="EB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39069">
        <property type="entry name" value="ECDYSONE-INDUCIBLE GENE E1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39069:SF8">
        <property type="entry name" value="FI17111P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01683">
        <property type="entry name" value="EB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588228" description="EB domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EB" evidence="3">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001877-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="250" mass="27809" checksum="F01A2DDD16ADA967" modified="2013-10-16" version="1">MTIIYFVVLSSLLVLVKGSIWPCEGNESCTATNSYCLLEEGKCACNNFEQIFSTNFEKCLPAVLYGDKCEDSNQCNLMPSGASCKEGVCQCQDGFTYVRGKCRKLSLLNDHCDENIDCYFGYDRQSVVCRANKCSCADGYYERYNDICRRKSMALNEACVVDLDCDIENGNGAKCENFKCILPSNNKRKESKEIAVQTSIDEIQLKTIVESQSDYSSTSPDENRTSASITSFTSQSNSIPINENENGNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF43</accession>
    <name>T1GF43_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02028221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02028226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001972-RA">
        <property type="protein sequence ID" value="MESCA001972-PA"/>
        <property type="gene ID" value="MESCA001972"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186952_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001972-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12408" checksum="6FCCBA13FFE03E7A" modified="2013-10-16" version="1">MIYQTLVTLHDIFSLFQMLNKIWWNSLLKPNVAALSNSAGMPSVYALLLFSRSKIALSTSALKKILKGRLLSFSSSEEKSSHILRTFCWSVIRLQFLSLQEEDDLEIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF53</accession>
    <name>T1GF53_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF53"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001982-RA">
        <property type="protein sequence ID" value="MESCA001982-PA"/>
        <property type="gene ID" value="MESCA001982"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161246_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001982-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12565" checksum="13DB3704A7830E2B" modified="2013-10-16" version="1">MFSSPKVPSPCVTLSISKRRRLRKKAAKIRKELEEKVKAEEQKKNTTMKPEIMQTATTDKSREQIKAEREAKKAAKQAAKTKSKEKSGENTVPATTNLNLLQKLNQKLQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFQ8</accession>
    <name>T1GFQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein krueppel</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02179960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02179964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GFQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002205-RA">
        <property type="protein sequence ID" value="MESCA002205-PA"/>
        <property type="gene ID" value="MESCA002205"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454631_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000064">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF104">
        <property type="entry name" value="AT-RICH BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="14">
        <location>
            <begin position="66"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="69815" checksum="0B61A53B4DD93CF7" modified="2013-10-16" version="1">IERRYAKYSPRWGLDPDLKDWITEKRGLAFCKICSQYLRVISGRKNLIRHKNSQKHITNEESLVKQQCRICLKNHDLKFMPSIWDRDKFTDMKIYRKIMHCTGIVIKREDTEKVTDKICLGCADQLNTSFNFLKTCEFSEKFFDSIAKGVPVEIPKEEFKLDTNEVYSEDESYGEDEYIDDGSDPTYAXXXXXXXXXGKTTIQAVVYEETAGINMEEGAYEAEIITDGGLGDFKDDQNIQILSGDIIGDEMKFEIKEPQFKRFRPNETTTTRQLQHQQQRVKSYAEEAKVVRNVAPEQQIYICDVCGNTYDKKSKLQTHIKNHSDEKPHECDDDQVEVCSDIEYNEEDGVGEIIFEEVISQEEDGQQTTEINIKLEPHTVEINPPLIEKADIKGYLEESKVIRNIPPASHMEEMFICDICGNTYDKKSKLSSHLKNHSDFKPHQCEMCNKSFKQSSQLTVHMRSHTGEKPLGCRYCDRMFAEHSSRIRHERGKEFPLPSVWKEFSSETYLTNHLNTISHRNTIKTMEIEDGCVGKQKRDLDEEYELSTDDIMNVEVEESTIIYSSGNEKEEVYQEVDEIDQTEKVEEYENDEGQVMQVIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFT3</accession>
    <name>T1GFT3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">USP8 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02096657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFT3"/>
    <dbReference type="STRING" id="36166.T1GFT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002233-RA">
        <property type="protein sequence ID" value="MESCA002233-PA"/>
        <property type="gene ID" value="MESCA002233"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1373639_0_0_1"/>
    <dbReference type="OMA" id="GDEEYAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP8 dimerisation" evidence="1">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002233-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="23148" checksum="A48C7FF2BF6C88C8" modified="2013-10-16" version="1">MAKKDLYLAKSFEELALKWKDIPNVKNVKYESLLISLKKLQKSANEHYLRGDEEYACILYMKYFTLLTLISKRDEFNSSIQKQIQTELGGHSVCMSLIERLEKIKNSLSSRYEQKYCQKRFKDPEIEISQTSSTKTPETENIDISSFSIISNAELFKRLKEKSTGTLLIDVRFKEDFEASNIVYSYCLNVPGNMYKKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGL7</accession>
    <name>T1GGL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02039676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GGL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002540-RA">
        <property type="protein sequence ID" value="MESCA002540-PA"/>
        <property type="gene ID" value="MESCA002540"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_569354_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045189">
        <property type="entry name" value="UBR4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21725">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21725:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="480" mass="55137" checksum="7EB62EC275D176D2" modified="2013-10-16" version="1">MASNIQCRKNLCEQGLIEELTQNNLRRGTVQIQDEVRHLLCLLTREVPDSCNKLLKLITDRVKITLNGVVPLANLDVAVRHEMILLEVLIAQDDFCWEQKLKVVFELFLIACRDSRGPIIAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXESWKGRMPKNNSSVFLGCKKKTPPPEDQGTQVDRKKRKRIAYLSEKYGKHWQRRAMEKDTKNAPLTLNASWIQPVLFNTNSLFGRQLACSLITCLSRTYERKKEILNLLTGFLKNVGDIGEASAEFLILYKNLAMDPPWLQYLALKGVLVYVAELLKNEIEKIHRLEDTSLSSDLAQGYALRRFVDLMSMFLDDPKIRQTYKGKLLGPVLQGYLSLRKLVVQRTRLIDDAQEKLLELLEEMTTGTEEETRAFMAILIDTVEQTPLNDIKTPVFIFERLYSIIHPEENDESEFFLTLDKDPQQEDFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGX8</accession>
    <name>T1GGX8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGX8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002659-RA">
        <property type="protein sequence ID" value="MESCA002659-PA"/>
        <property type="gene ID" value="MESCA002659"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1637359_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="19265" checksum="AFC430B3F2765A09" modified="2013-10-16" version="1">MDFQNFQDSLEFEFWCHLLRTTPLELTQMPDHELKNRIHRVQKKYSNPKYKHQRLQELQKSLREFKRRKSNRKLCNDKFSTVSETMGWVRNLLSPDDEDLDEESCPLSNDLDEDLEQESCPLSRSRSCDMSNTRSRTCDLSAEMSPLSGNRSMSRSKSQFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHH0</accession>
    <name>T1GHH0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02177181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHH0"/>
    <dbReference type="STRING" id="36166.T1GHH0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002871-RA">
        <property type="protein sequence ID" value="MESCA002871-PA"/>
        <property type="gene ID" value="MESCA002871"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062828_3_3_1"/>
    <dbReference type="OMA" id="MPIAIHR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000020">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="15">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002871-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="13432" checksum="E62488C2BF1E8989" modified="2013-10-16" version="1">MSGRGKGGKVKSKSKTRSSRAGLQFPVGRIHRILKKGNYAERVGGGAPVYLAAVMEYLAAEVLELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLSGVTISQGGVLPNIQAVLLPKKTEKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL6</accession>
    <name>T1GHL6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-8A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL6"/>
    <dbReference type="STRING" id="36166.T1GHL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002923-RA">
        <property type="protein sequence ID" value="MESCA002923-PA"/>
        <property type="gene ID" value="MESCA002923"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="OMA" id="FKERCNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002923-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="18002" checksum="C98C26476327F1FD" modified="2013-10-16" version="1">MALDYAATYKVLVLGDSNVGKTCIVHRFCDEKFYDTYISTIGIDFKQKLINLDGVPVKLQIWDTAGQERFRTLTTAYYRGAMGILLMYDVTNLESYNNLSYWLRNIQENASPDVVRVLAANKCDCTENQRMIDKHDEPELDIQENSSFVFKERCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHQ2</accession>
    <name>T1GHQ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02153904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHQ2"/>
    <dbReference type="STRING" id="36166.T1GHQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002960-RA">
        <property type="protein sequence ID" value="MESCA002960-PA"/>
        <property type="gene ID" value="MESCA002960"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_214595_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002960-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="5196" checksum="DEB2BD29ECBDA175" modified="2013-10-16" version="1">MLSLQAEALSKGFAPPPSSGLSSVQSPSSAPGAPLAALHGLQPWAEQHSTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHV6</accession>
    <name>T1GHV6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RyR/IP3R Homology associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02034474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GHV6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003018-RA">
        <property type="protein sequence ID" value="MESCA003018-PA"/>
        <property type="gene ID" value="MESCA003018"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_286142_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RIH" evidence="9">
        <location>
            <begin position="55"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="10">
        <location>
            <begin position="840"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003018-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1081" mass="122613" checksum="E4B616674906F3F6" modified="2013-10-16" version="1">FFKPFPLAPTNDKSTEHNLSADYRASLTTEQLNEYQKVKDILIRMNKFCITTGSNIKPRKHEQRLLRNVGVHTVVLDLLQNPYDEKDDELMKELMCLAHEFLQNFCLGNQQNQVLLHNHLDLFLNPGILEAKTVCAIFKDNLALCNEVTDKVVQHFVHCIEIHGRHVVYLQFLQTIVKAENQFIRKCQDMVMQELINAGEDVLIFYNDKSSFNQFVGMMQKHKIENNGGPLSDDNPLKYHVELVKLLACCTMGKNVYTEIKCNNLLSLDDIVTIICHPDCIPEVKDAYVDFLNHCYIDTEVEMKEIYSSHHMWNLFEKSFLVDINDLNSPNPPEQKTIINYVMNGVMNILNTFFNSPFTDTAIIVQSRQMIFVQILQATCKTLTESAKLRNIAIPSELELQVATMSSKTALLTRQTTKWLLASKQPKFDTPQSNQHIRMDRSIIEGLQDIVSLLEDQLKPLVEAELSLLVDVLYRSELLFPIGTESRRKCESGGFIRKLIKHTEKLLEEKEEKLCVKVLRTLREMMAIDENYGEKGDNLRSTLLIRYFGKGFTTKPSDSNENRKLHLVTHGPGAKYLNRAVTHGPGAKYLNRAGKTLHEVQNHLDKEGASDLVVELVIKSVHSPSIFVEAIELGIALLEGGNPTIQKGMFSKFLSGDLNQAFFKVFFEKMKDSQQEIKSTVTVNTTDIAAKAHENKQDMSLELEKISRKQGLKSNGVIITDELKKELNNAGIATAKAYSHARNISPGGEDNNGLMHVGNPLEDILAEKIEKHKDKDEKNKLSXXXXXXXXXXLAEKIEKHKDKDEKNKLSSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQNLLRNQNNKTNYNLVSETLMFLDCICGSTTGGLGLLGLYINENNVALINQTLETLTEYCQGPCHENQNCIATHESNGLDIITALILNNINPLGENRMDLVLELKNNASKLLLAIMESRGDSENAERILYNMNPKQLVEVACKAYHQEEMIDEPEHDEDGNDDDDDIGVSPREVGHNIYILCHQLAQHNKELSNLLKSTEFTLDSKMNQALLYYATHTAQIEIVRNDRTLEQIVFPIPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIX0</accession>
    <name>T1GIX0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase 47</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin thioesterase 47</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-specific-processing protease 47</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02034557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02034562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GIX0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003407-RA">
        <property type="protein sequence ID" value="MESCA003407-PA"/>
        <property type="gene ID" value="MESCA003407"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002928_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045578">
        <property type="entry name" value="USP47_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF702">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25985">
        <property type="entry name" value="Ubiquitin_USP47_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19718">
        <property type="entry name" value="USP47_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="225"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="518"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="903"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1292"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="908"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="925"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1292"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1317"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1342"/>
            <end position="1359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1359" mass="153781" checksum="7DD602710C6AB6AC" modified="2013-10-16" version="1">MVQLPDEEKTICVVYDTTPSSTQKRMNLVVRSNYDIRKLFETVKTQYQYESFQLVMQASDEDEINRVIYLNDKPDLLLYDVPGFCPGHKNQLIILPPNTWENKEELDLALKSLPSHFTSPTSSVLLTPPAEIQTSTETSTANSFVGQGIDSSSANMSPVTPETFDYYLALGASASPTEEKAQFNSIYSSLPDECPRDQDPIETNSFEYNQENEHEDYQRENLPELNLNSKFGSSSQYEPVALPIPAAPVFAPSSFMTPEFRNALYRWEFDNDKEDKNIPYQLQKLFLNLQTNPKTAVETTDLTRSFGWDSTEAWQQHDIQELCRVMFDALEVKFKQTKQAKLITNLYEGKLIDYVKCLDCNTEKSRADSFLDIPLPVRPFGSQMAYSSIEEALRAFVQPETLDGNNRYFCEKCNKKCDAHKGLKFKEFPYILTLHLKRFDFDYQTLHRIKLNDKVTFPQTLNLNAFVNSAKTQTLQNNNNNNNNNNLESVKTNGIVMNGINNNTDDCSITDSGAAMEEDNSSGIATTASSSQHENDMQDDDEGISVDTSDQPVKKNDKGPYNYELFAIMIHSGSATGGHYYAYIKDFESKEWFCFNDQSVSGITQEDIQKSYGGCSGKSYYSGMFTSSTNAYMLMYRQIDPKRNESAFRREQFPKHILELLEKMSSEEESKRQYTSKVSDIIKSKVYVYNKKSKKMKTARVHLARSYDIATALESAYETLGLIESLDGEHNKDRTIGSIDDNSPVSPNFLLETRDEKEAFVAYKREEVQLKVFKVDLDTKDINGPYFIRTLGEDNMKQAIAKTFNIDAGKVLTYYRCCKTNAKVFISESKEEINEDAESDIVDNYTSFVVDNFTGMTYFYITHPSTDSKSLNILGIPSYSEQQKQEQKSCDVVDAVMMSSGCSGSATENSNSEDSSLSDGDRTLVGVSGSSSSHSPQVLTPEEEGVSSGQIEEALSKKFRFVELNNECDYNCFSSLVHSPISYSSSTDNDDSSAAQNGPSKTFKIFVDRNTSIGVLKLLLAPIIQVPTDYFKIVRRSASSIRSEEFALSQKMSFFSEGEELKIELGKILKKDEMQAKVFYMKLSDLNNESGKFTNVCNCVVNSNMSALDVKKMLVDKLHQLEPEKYKHLTVKNCRLWEKGIRNLVKIMTDDQKLKFETAVKNFSWEFLVQECEGDVDPNPPEDSLTLFVRRWYPSTLELGKFEEVTMLRGSKIQNTLSSISGIPESDIXXXXXXXFLISIIFQLNAMIPGNNTSLLNINTSMSWFTNEASLDNYPLLQSACGTIYFYKNSKEEPKELTPEESKELNLKEKARLDKVSGGTSTSTSSYYTSRRERALKIYVDSSPKSPTKSTATSNIENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJB0</accession>
    <name>T1GJB0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003555-RA">
        <property type="protein sequence ID" value="MESCA003555-PA"/>
        <property type="gene ID" value="MESCA003555"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1717806_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003555-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="17442" checksum="361C5A345552D7B1" modified="2013-10-16" version="1">MRASNSPISNMSKENKSNQILGYADDIDVIGRTKLDVESEKVASSYGLMGSEITSNNDITVEVKRRIVLANRCLNRKTLRRKRFKKKGGGCEIAYTEEKESGEKVLREHKPSAESKNKNGDLITDKEEILMQWENFFKELLIGNESRKEISTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-11-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJC6</accession>
    <name>T1GJC6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJC6"/>
    <dbReference type="STRING" id="36166.T1GJC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003572-RA">
        <property type="protein sequence ID" value="MESCA003572-PA"/>
        <property type="gene ID" value="MESCA003572"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640940_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003572-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8493" checksum="9849DE1056A4C793" modified="2013-10-16" version="1">MLLSFAFCLSDISHIITGQRSETLDNVGTTGLVLICSPSPALRKSEIEYYAMLEIQSPILGSSCIELGTAYEKYFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJJ9</accession>
    <name>T1GJJ9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003648-RA">
        <property type="protein sequence ID" value="MESCA003648-PA"/>
        <property type="gene ID" value="MESCA003648"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673935_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576976" evidence="1">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003648-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="7923" checksum="4D00BE09615597CA" modified="2013-10-16" version="1">MKFAIVFAALFALAIAGPVANEADIVRSDSEVGPESHHYEFETSDGTHAASDGTLLNAGSDAEFTNMLDPSLMSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJM8</accession>
    <name>T1GJM8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02150285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJM8"/>
    <dbReference type="STRING" id="36166.T1GJM8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003678-RA">
        <property type="protein sequence ID" value="MESCA003678-PA"/>
        <property type="gene ID" value="MESCA003678"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030703_1_1_1"/>
    <dbReference type="OMA" id="EHMPRSF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.20:FF:000002">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21432:SF20">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21432">
        <property type="entry name" value="ACETYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="3">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="4">
        <location>
            <begin position="273"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003678-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="438" mass="48453" checksum="D86752795C48CAFC" modified="2013-10-16" version="1">MLSRQLLRRTFFTYTNELSYPIPNRKPPIVPVEEAVSVIKSDDVVFLSSAYATPEYLVNGMVKHAKAKDLKNISLPVLLAFGEGAYCKPEYKKHFRTNSFFIGSNIRKSVAEGDADYMSIHLQDIPKVFNNGIIKPDVALIHVSPIDEHGFLAQINKQMVRTFGDSLIHSSHFDYAVEIDEPLMELKSSDPSDDERKIGHLIAENLVEDGSTLQMGIGKIPDAVLEALGNHKNLGIHTEMFSNGILPLYEKGCVTNSEKRNHRGKIVSTFVMGDRNLYDFVDNNPMVELYGSDHTNNTKVISANPKVVAINSCIEIDLTGQICSESIGTGFFSGFGGQVDFMRGAAEGFDGKGKPIIAFTSTTAKGTSKIVPTLKPGAGIVTSRAHVHYVVTEYGIACLFGKNMRQRAYELIKIAHPDHRESLEREAFERLKVMPSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJR3</accession>
    <name>T1GJR3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116171">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116172">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003713-RA">
        <property type="protein sequence ID" value="MESCA003713-PA"/>
        <property type="gene ID" value="MESCA003713"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2742952_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003713-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7914" checksum="E70EE77A41810981" modified="2013-10-16" version="1">MSMLIIQEHQMMSATLRRSTFSIEALVAVTRRMNQKYSLEYKIETTSFSEAFKGGASSASILSCCVKTLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJS9</accession>
    <name>T1GJS9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192768">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003733-RA">
        <property type="protein sequence ID" value="MESCA003733-PA"/>
        <property type="gene ID" value="MESCA003733"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2017861_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003733-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14259" checksum="993CA087D7319C92" modified="2013-10-16" version="1">MEISGSTISCSNWTKGGDWRMFFLDRSKARSLRFSKFDGSPRSLASLKILGGLDLARLLRSLTWPHLFLGLDMGRRGPKLVVQTFFDPFQYAYDFKRNLIEQSWPHIFLDCIGIFRSHAIWLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKA8</accession>
    <name>T1GKA8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003927-RA">
        <property type="protein sequence ID" value="MESCA003927-PA"/>
        <property type="gene ID" value="MESCA003927"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673923_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003927-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8538" checksum="42A0FCE230B10867" modified="2013-10-16" version="1">MNKGKKRENGSNSSSNGPKKPRNFADDDDDMDFDMTAFEDEDNNIDYADLEGKVLKHCKHLKNGLVQICHLLIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL58</accession>
    <name>T1GL58_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CAQQ02098718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL58"/>
    <dbReference type="STRING" id="36166.T1GL58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004248-RA">
        <property type="protein sequence ID" value="MESCA004248-PA"/>
        <property type="gene ID" value="MESCA004248"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_967375_0_0_1"/>
    <dbReference type="OMA" id="DEIWSHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF83">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004248-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="34464" checksum="3B56B61ADE2B742D" modified="2013-10-16" version="1">MIDRIQPSVKTYCHFWFDGQKEPHIVKSFEYKYIWHNKWGNYKQGIYQPYLIACRIPKPFHGIVPASVSLVEKECETATNNLRVIYNRPPDDHKKGFAVCVKGLDFLYDDLSVRLIEWIELLNMLGADKIYFYNLQIHQNISKVLKYYEKEGKVQLTQLTLPGGQPNVPGFQHLYLTKKTNHKRQNEIIPYNDCLYKNLYLYDYIALLDIDEVIMPKHGDMFWAETMKRVLPKAKQVRADGYHSYNFRNVYFFDQQHHDHGWYPEIPKYMHMLQHVHRAKTIRNRTNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLM3</accession>
    <name>T1GLM3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLM3"/>
    <dbReference type="STRING" id="36166.T1GLM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004433-RA">
        <property type="protein sequence ID" value="MESCA004433-PA"/>
        <property type="gene ID" value="MESCA004433"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1312902_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047016">
        <property type="entry name" value="RGS6/7/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746">
        <property type="entry name" value="LP21163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746:SF5">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004433-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="23048" checksum="C8BE803B12504A44" modified="2013-10-16" version="1">MDIVQKGLKNPSRFTYDAAAEHIYMLLLKKDCYPRFIRSEHYKNLLATGIQPSHKKRFFNFGNVGVRKTKNTTAAASSQPATQTQLIPYTGSSCGPGPSIASLARRRGSDRSLTGSAHELAVIGVKDVSSKVPHSHSQSNLSEIPYGEAIYRGDMPMPCETDDRKHPTITTSTTVQVCPWDEDAKLPPTPTCPRKSSNASDKKVAVDKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLM4</accession>
    <name>T1GLM4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02007090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLM4"/>
    <dbReference type="STRING" id="36166.T1GLM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004434-RA">
        <property type="protein sequence ID" value="MESCA004434-PA"/>
        <property type="gene ID" value="MESCA004434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="OMA" id="EQSWEIG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000218">
        <property type="entry name" value="facilitated trehalose transporter Tret1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="20"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="444" mass="47874" checksum="A82773166153EDD5" modified="2013-10-16" version="1">MATPAPAPTNKEKYFPQVLAALVASGGALVAGTVLGWSSQLFDPLVKEGAYKFNITEFEFGWCGSILNLGAAATCIPISFICDLIGRKFTMLSLCGPLLLGWILVTAAVNPIMVMFGRFFLGMGSGGFCVAAPMYTGEMASKKIRGLLGTFFQLMITIGILISNILGAYVKLLVFHLLCLVITTIFTILFIFLPETPAYLISKGKDEKAEKNIKTLRGKNFDPKTEIADLKADLEKRKAHPSNECKSLCTKYGLTSLFIGMGLMFYQQVSGINAVIFYASVIFGASKTSLDMSVCTVIVGVVQVIATCVSAAVVDKMDSVADLGWLPLTSLIVFIIMFSFGYGPVPWLMMGEIFDPSFKSIACGINGTTNWTLAFIVTAVYGPLNKAIGGGPVFWIFSGLTLSGLAFVFFAVTETKGKSLAEIQEIMKSKKEKAKDAETKDAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLQ2</accession>
    <name>T1GLQ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLQ2"/>
    <dbReference type="STRING" id="36166.T1GLQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004465-RA">
        <property type="protein sequence ID" value="MESCA004465-PA"/>
        <property type="gene ID" value="MESCA004465"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3178130_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF60">
        <property type="entry name" value="RH50, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004465-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="47" mass="4679" checksum="912475E981A5D1DF" modified="2013-10-16" version="1">MVHVQNSTLAGGVAVGSVCNLLIGPHGAVLIGIVAGTISVLGYRYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM92</accession>
    <name>T1GM92_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004661-RA">
        <property type="protein sequence ID" value="MESCA004661-PA"/>
        <property type="gene ID" value="MESCA004661"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1965057_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="13764" checksum="16357AE799BF771A" modified="2013-10-16" version="1">MNCFDNSSCRPDQMNNYLGTSQNLSQDYSKSYNGTERSENNSPNNNHPIIPQISAMPPSHLSNTPPVLPNCNDTCSPVSISSSTRGHDGSAAANMNYVPMAMKTPYIFKEEPGTSVSSGAHSYSNLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMA6</accession>
    <name>T1GMA6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WASH complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC53 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMA6"/>
    <dbReference type="STRING" id="36166.T1GMA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004675-RA">
        <property type="protein sequence ID" value="MESCA004675-PA"/>
        <property type="gene ID" value="MESCA004675"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117940_0_0_1"/>
    <dbReference type="OMA" id="FGNKMED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019309">
        <property type="entry name" value="WASHC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015">
        <property type="entry name" value="PROTEIN AD-016-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015:SF0">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10152">
        <property type="entry name" value="CCDC53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004675-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18860" checksum="260871127407F3AB" modified="2013-10-16" version="1">MENGYSMLDIDKKQIPPIQKKRILAFVNHFLIQTTKFLNKFANECETKFIEVERKIQTVEASLVIVESKLNSIPGIEALAPVKAVAAPTPVPECVTNHTGEHSKDIPDSGNVPETKQETVVDEKPEPEGIKACEDQRYKKYFKMVQFGVPPSAVKQKMSAEGFDASILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMH1</accession>
    <name>T1GMH1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMH1"/>
    <dbReference type="STRING" id="36166.T1GMH1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004745-RA">
        <property type="protein sequence ID" value="MESCA004745-PA"/>
        <property type="gene ID" value="MESCA004745"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530062_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004745-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9827" checksum="AF97CD2957BD22A5" modified="2013-10-16" version="1">MTYGTTAAPLLSDRVLKQLALDYKSTYAIVAKILLDDFYFDDCLHAELSLPELIYAKRELEELLKLAVLQLQKWTSNGWYWFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMI9</accession>
    <name>T1GMI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMI9"/>
    <dbReference type="STRING" id="36166.T1GMI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004764-RA">
        <property type="protein sequence ID" value="MESCA004764-PA"/>
        <property type="gene ID" value="MESCA004764"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043579_1_0_1"/>
    <dbReference type="OMA" id="CSRERNI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000103">
        <property type="entry name" value="DnaJ subfamily B member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004764-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="238" mass="27574" checksum="DEAA0AA7733F57B2" modified="2013-10-16" version="1">FLSISRCKDYYEVLNVTKESADSEIKKAYKKQALLLHPDKNKAPGAVEAFKAIGNAVAILTDPEKRKAYDLHGSDEPFNPHGGGQQGGMGRRHYTNEYAYTRGFESDVTAEELFNMFFGGAFQQTNIYNRQRQFRQREYHREREQQSYYSGLINLLPILLLVGLSMMSSFFISDPIYSLQPSHKFSIQRRTNSLKITYYVKDNFHTEYQGSVSRLEASVEEDFLNHLRHSCSRERNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN20</accession>
    <name>T1GN20_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02054640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN20"/>
    <dbReference type="STRING" id="36166.T1GN20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004959-RA">
        <property type="protein sequence ID" value="MESCA004959-PA"/>
        <property type="gene ID" value="MESCA004959"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006586_13_2_1"/>
    <dbReference type="OMA" id="EYPRDID"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004959-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="293" mass="32928" checksum="A89089E94BD27DE0" modified="2013-10-16" version="1">MVWFHGGGWECGSGISDFYGPDHLLDKDIVYVSGNFRLGPLGFLSTETLDCPGNNGMKDQVEILKWVKENIARFGGDPNSVTIFGESAGGASVTYHMMSKLSKGLFHKGIAQSGTYHNPWAQPAHKGVAARRAYKLAKLLHCENSENDWQRMLDCMRKLPADAVTAKLYDFYEWDYEPMIPFPLAVEPNHPGAFLTEYPRDIDHSKLHSLSIPLMAGATSGEGAMRSAAYLNNPELLKAMKEKYKHILPIMLNYDHHDIDKQKEVTAAIDKFYFKNGHNYHMGDHDNFTDVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN22</accession>
    <name>T1GN22_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004961-RA">
        <property type="protein sequence ID" value="MESCA004961-PA"/>
        <property type="gene ID" value="MESCA004961"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064137_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004961-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13497" checksum="7648A1B6FA2B0764" modified="2013-10-16" version="1">MGLLGADPKDAEDFDDENEKDVIDKEDYSYSLNPWALSRVLLTNFILTAGRFRQGDAFSFSFFNILIELIKVSANMYFGNIIINKSSLAADIVFIGRSYDNMIESFLRFKHVAESGGYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN76</accession>
    <name>T1GN76_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN76"/>
    <dbReference type="STRING" id="36166.T1GN76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005020-RA">
        <property type="protein sequence ID" value="MESCA005020-PA"/>
        <property type="gene ID" value="MESCA005020"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024950_1_1_1"/>
    <dbReference type="OMA" id="RIFWIIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005020-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="389" mass="45535" checksum="FC168ABF73BD9A32" modified="2013-10-16" version="1">MGENFTISSRNRWSTNILNQYSSHSTITGIQYLAEPRRPLIERIFWIIMFLAMTIICVKLTWESYIKWKDSPVLISPNNQFSSVLEIPFPAVTICPHQKFNMTKKNMTDFILRLYNKYLNMTDENNQLIFEVAQQICPALNEDLLRFFFITFPDKYVDPDIFMSTMIDLSPTYKSIFLYRFWETKDIDGEFQPFLHKEGICFTFNSLDTSDIFRNGSNPHKILLDKLKYQLVEHKKSSPFNGTRSGKGSGFRVQMLNDLDNNCEICQEMNQGAEIAIHSPDEVPDFTNYVSINGKKNVLVSITPRVMQISDGLKYVDFSKRQCYLKNEKYLQHFKIYTQANCEFECLTNLTLKMCGCVKFYMPRNETTKFCTRENYCTSTASENLSFWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNE7</accession>
    <name>T1GNE7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005095-RA">
        <property type="protein sequence ID" value="MESCA005095-PA"/>
        <property type="gene ID" value="MESCA005095"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388726_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005095-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="11153" checksum="6B895AFA35CA9567" modified="2013-10-16" version="1">MENIRINRDGKCLQFMEIMDKQHRHCCSLHKTLFEWDSNYLTKYIDKNKLIMNQGWTLSQYGLSYISNMNNIDNYGLLKKNIDPKLLQSSSSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNR5</accession>
    <name>T1GNR5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02127105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNR5"/>
    <dbReference type="STRING" id="36166.T1GNR5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005224-RA">
        <property type="protein sequence ID" value="MESCA005224-PA"/>
        <property type="gene ID" value="MESCA005224"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072097_0_0_1"/>
    <dbReference type="OMA" id="WQFDVEI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="16"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005224-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="23570" checksum="9D58D36E88B7108E" modified="2013-10-16" version="1">MATSTAQETKNCITLRGSATIIAEYLKYGINSILFQRGIYPPEEFDNTQQYGLTILMSKNEKIQSFLTNVLSQTEEWLSKNLLNKVSMVITNAHTKEVLECWDFKVQAQPGDGEDNSPMNLTSSKDLKRVQSEIRDVMRQISATVSYLPLLDCICTFDVMIHTLQNCELPSGWDETAGIFIQNAQAVQLRSFSTGLHKVDTVVNYKMTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNW1</accession>
    <name>T1GNW1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005272-RA">
        <property type="protein sequence ID" value="MESCA005272-PA"/>
        <property type="gene ID" value="MESCA005272"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818003_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005272-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="15402" checksum="1E5B890BF7F47245" modified="2013-10-16" version="1">MEACIDLIDKICGNVSRGQFVGRQQIVAVDGKLSEEKVINIGVPQESTSDLEMESIKETISDVLVMSPMMAACLARDSKVADGQSALPKMIAFKRAFPLLKSCSETLPDFNTLAALSIAWKNSCFNSRFNGHDSSTQSAVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNY7</accession>
    <name>T1GNY7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02103821">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNY7"/>
    <dbReference type="STRING" id="36166.T1GNY7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005300-RA">
        <property type="protein sequence ID" value="MESCA005300-PA"/>
        <property type="gene ID" value="MESCA005300"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1580299_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="19591" checksum="5D6985E7EB49991C" modified="2013-10-16" version="1">MDNEENKQLSGTSSPPFLKFYYLRNKIKVNTNYLMKGISQKKNLEQRKDATLMELEHNLPGFQSSCHQPFCPYQYDVPYKQSPFFEEQLTAFQVWLNRASDKRTPPEQLPIVLQVLLSQVHRLRALELLGRFLDSGPWAVHLALGVGIFPYVLKLLQSAAKIIKTYFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPI2</accession>
    <name>T1GPI2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPI2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005513-RA">
        <property type="protein sequence ID" value="MESCA005513-PA"/>
        <property type="gene ID" value="MESCA005513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3353615_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="37" mass="4128" checksum="121F8AB97B7E279E" modified="2013-10-16" version="1">MKMDPSIEDEDEKTEQEEDQSNQQVVAEAMVELSGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPI7</accession>
    <name>T1GPI7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3 12">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3 12"/>
    <dbReference type="EMBL" id="CAQQ02185501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPI7"/>
    <dbReference type="STRING" id="36166.T1GPI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005518-RA">
        <property type="protein sequence ID" value="MESCA005518-PA"/>
        <property type="gene ID" value="MESCA005518"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009311_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017419">
        <property type="entry name" value="MAP3K12_MAP3K13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF304">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 13-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038165">
        <property type="entry name" value="MAPKKK12_MAPKKK13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="58"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="410"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="550"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="700"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="702"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="734"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038165-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005518-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="809" mass="91715" checksum="493435B08AA49AC1" modified="2013-10-16" version="1">MGGGVGGIHFSGSFPEKQFSWFHLLNCIKPVFSFINNVNGTEHKSTKNEDWEIPFEAIKDLEWIGSGAQGAVFSGKLLNEMVAVKKVRDDKETDVKHLRKLNHENIIKFKGVCTKDPAYYCIVMEYCPYGALQVILKDETNMSPQRLVSWSKQISSGMEYLHSHQIIHRDLKSPNILIGTNEVVKISDFGLCKTWDEKSTTMSFAGTVAWMAPEVIRHEPCSEKVDIWAFGVVLWEMLLCEFPYKDYDSSAILWGVGNNTLSLPIPKTCPEGFQYLLTLCWKIKPKHRPSFPEISKHLDIAGDFLLKNVNDEEFFENRRLWKEEVGNKLIQSTKSNMNVEHDLIKRRASELSTARDIRQIYIAKLADTNKLYIQLSECKAQLEEKEKEINHKREKKLSIYNKNFRRFGPTIKKMNNLKKRSPPPLPRVPGKESTSPDPKTTPESPENFNLYATLDSSARPTSTVVRSSKRRTHRRGASGSYLEFQKHHPVRDVKYTSEPENNIQKKKKLVDMMTQTDAMEISEVSDVSPNALSAYRTLMNTLESKKEDEISASSISRVPAPITQQPQSSLSSPTNNNTNSDVTFQDACSSPDQLIIDDALNSNERLDMLKVTQSDDEHLERLERQVSDFVNGTIDNGNNPQLSKKKVLLCMNENSNQATIIRTRNEYLNANSESRDDSWSESDNEEKKYVYYLRRRDEENTSECSHSPSSQHSTLESNPDVPEAYKKMHHKSANSKSTSSSTTNTPGTDSDVPSSDDSNSDCEELDNRPTINTKQNLTTADFEESNGNIRCSDFISIPTLPGAGSIDMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPL2</accession>
    <name>T1GPL2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">6-phosphogluconate dehydrogenase NADP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIBADH-related family. NP60 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02012145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPL2"/>
    <dbReference type="STRING" id="36166.T1GPL2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005545-RA">
        <property type="protein sequence ID" value="MESCA005545-PA"/>
        <property type="gene ID" value="MESCA005545"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_587401_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF2">
        <property type="entry name" value="CYTOKINE-LIKE NUCLEAR FACTOR N-PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="3">
        <location>
            <begin position="255"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005545-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="466" mass="50723" checksum="8EE4A77F0502055D" modified="2013-10-16" version="1">WVEPENIHAFDGPQKENFIKTGKGRGFKEAIEECENYKTNPTILEGVLEPAEELDTNAEFDRIRNGPNPSTSENIPTSSSGMELDESTGNATAGASSSASASKRKRSSMASSNNEKATPPQRGRKNSQKSSTARKTKGAKSDQTADSTTPSAKATPKRLKRDQSLDDLASNDSTNDFDQTPHNQSIRSRASQQHHRQTNHNDLQSQQPYVSGAGGAAGGRSFLNRSVVMREEEPVIDMKPNSSIINRKIEPTSKRIGFLGLGIMGSTIAKNFLQSGHKVSVWNKHARNVYRLNYMELPFEKHLQMFARNVTSFSHLVFGNCGILDRDILTDKAYVEMSTIDPETSNAIANGIHVKGGRYLEAQIQGSKLEAEAGTLVVIAGGDRSVYDDCQSCFQSISKNTFFLGEAGNATKVNLIIQTLAGTCLAGLSECLALADRFSILPSDIMDIIGLTSMNSHFLLSKAETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ04</accession>
    <name>T1GQ04_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar membrane-associated protein Iml1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020821">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ04"/>
    <dbReference type="STRING" id="36166.T1GQ04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005701-RA">
        <property type="protein sequence ID" value="MESCA005701-PA"/>
        <property type="gene ID" value="MESCA005701"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035719_1_0_1"/>
    <dbReference type="OMA" id="NTCAYVT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar membrane-associated protein Iml1 N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005701-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="384" mass="45098" checksum="2B9A24E6B90B25F4" modified="2013-10-16" version="1">MPHNTCVYKNKKIEYTEMPIRCQVYEMWSQGERVNCGVITNDTKIVFRSSTSMVYLFLQMSSEMWDYDIHGDLYFEKAVNGFLTDLFGKWKKIGSNHEVTIVLFSRTFYDAKTLDEFPEHMKECLQQDYKGRYYEDFYRVAIQNEKNDDWCAVSTQLRKLFTTYQETVLRCHDRQHFKIPKAYNSTATQGNFLEVLNISLNTFEKHFLDRSFDRTGQLSVVVTPGFGVFEVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKESLTTVDDYSLPHWINLSFYSTNKKVSYSTFMPRIKIPTLKSENKENVPKNKYEHSFLSCSQSENIHNSLFDYDEYDAQIFQPPPAQSTCSLQRIVRAKKLLFLPWKRTFRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQM7</accession>
    <name>T1GQM7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005938-RA">
        <property type="protein sequence ID" value="MESCA005938-PA"/>
        <property type="gene ID" value="MESCA005938"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3280067_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005938-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="41" mass="5184" checksum="F58D4B8FD25461F3" modified="2013-10-16" version="1">MALNVIQECRRRNVDCIKIRTWFFLELRKLFLNWIWLAKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRC3</accession>
    <name>T1GRC3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006203-RA">
        <property type="protein sequence ID" value="MESCA006203-PA"/>
        <property type="gene ID" value="MESCA006203"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870172_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7291" checksum="360E1F7CBEC37757" modified="2013-10-16" version="1">MDDDDEEEQLITSRFKHHFSNQPQTTCDLFLKACFLNSTRPVALKLSTKASRFSPPQTSEAPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSA2</accession>
    <name>T1GSA2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02044051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02044053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GSA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006554-RA">
        <property type="protein sequence ID" value="MESCA006554-PA"/>
        <property type="gene ID" value="MESCA006554"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1351893_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF23">
        <property type="entry name" value="AT30656P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="15">
        <location>
            <begin position="37"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006554-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="22567" checksum="0B4A247420BEC3CB" modified="2013-10-16" version="1">MSYDGNGVLPIRNGLDKQVKKDKKQGLKEAESVVYVMTEFALELMKSLEAFNRENFQQEDDSLNHQFQLRIGISHGKVMAGVVGSSKPLYDIWGNAVNMASRMDSTGXXXXXXXXXXXXXXVNMASRMDSTGIPGYIQVTEETAKILQSFSIKCDYRGLTFVKGCDNIPTYFVAINDDLSFIRKSITGPNLTYENVKAAGMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSB1</accession>
    <name>T1GSB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="EMBL" id="CAQQ02038961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038965">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSB1"/>
    <dbReference type="STRING" id="36166.T1GSB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006563-RA">
        <property type="protein sequence ID" value="MESCA006563-PA"/>
        <property type="gene ID" value="MESCA006563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013477_0_1_1"/>
    <dbReference type="OMA" id="MGQGFAP"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23433">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000047">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000005">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF101">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="21">
        <location>
            <begin position="444"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="576" mass="65982" checksum="7DB4EE64F3C98E8E" modified="2013-10-16" version="1">MDGGVKTKRMTLIMRAGNNAVAEEVEHPDPEDDLENIIGNNDEDGGVSVPLTYRSALLKKWRQAPVVKPGAGNHGEMGKPVKIPDEMQDLMKEKFKENQFNLLASDMISLNRSLTDVRHENCKRKFYPPKLPTTSIVIVFHNEAWTTLLRTVWSVINRSPRTLLKEIILVDDASERDYLGKKLENYVATLPVKTFVLRTEKRSGLIRARLLGAKHVAGEVITFLDAHCECTEGWLEPLLARIVQNRRTVVCPIIDVISDETFEYVTASDQTWGGFNWKLNFRWYRVPQREMERRNNDRTAPLRTPTMAGGLFSIDKKYFYEIGSYDEGMDIWGGENLEMSFRIWQCGGILEIIPCSHVGHVFRDKSPYTFPGGVAKIVLHNAARVAEVWLDEWRDFYYAMSTGARKASAGDVSERKELRTRLKCKSFRWYLENIYPESLMPLDYYYLGEIRNSETQTCLDTMGRKYGEKIGVSYCHGLGGNQVFAYTKRQQIMSDDLCLDASNANGPVNMVRCHNMGGNQEWVYDADDKTIKHVNTGNCLQRSPRDDPTTPLLRPCDYSKGQKWLMQTKFKWQAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSJ0</accession>
    <name>T1GSJ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSJ0"/>
    <dbReference type="STRING" id="36166.T1GSJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006650-RA">
        <property type="protein sequence ID" value="MESCA006650-PA"/>
        <property type="gene ID" value="MESCA006650"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1628943_0_0_1"/>
    <dbReference type="OMA" id="NDICARW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051697">
        <property type="entry name" value="Patched_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796">
        <property type="entry name" value="PATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796:SF92">
        <property type="entry name" value="PATCHED-RELATED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006650-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18739" checksum="93636744AEEB198A" modified="2013-10-16" version="1">MLRLEVFEELRKLDNLIQNASVHYDGEFYSYNDICARWGDECFSNDILNLDQILGEFQAGELNLTFPFMLNPVTWDSHVFPVFFGGTKLDANQNIESVPAIQLVYFATADTKKQDKKGAEWEETFLEIVGKAENSGYFKHISVAYFASRTLDNELEKNTQTML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSL3</accession>
    <name>T1GSL3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182230">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSL3"/>
    <dbReference type="STRING" id="36166.T1GSL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006673-RA">
        <property type="protein sequence ID" value="MESCA006673-PA"/>
        <property type="gene ID" value="MESCA006673"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457369_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006673-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="9551" checksum="79062A5104805CB9" modified="2013-10-16" version="1">MLAPESILSPSPDDEETILSEDPTPAETTHQGNEKPSTVVILAGVIAVAAVIVTSIIVAGIIVVCKHRPKPPDPQEVRKRMRVIDGFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSP0</accession>
    <name>T1GSP0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02179484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSP0"/>
    <dbReference type="STRING" id="36166.T1GSP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006700-RA">
        <property type="protein sequence ID" value="MESCA006700-PA"/>
        <property type="gene ID" value="MESCA006700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068550_5_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19741">
        <property type="entry name" value="bHLH-O_ESMB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000009">
        <property type="entry name" value="Transcription factor HES-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="17"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006700-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="107" mass="12310" checksum="7CFCB9C694A60C31" modified="2013-10-16" version="1">MAIEGHNLNNMSKTQHYRKVTKPLLERKRRARMNHYLDELKDLIVETMEAQGEHVTKLEKADILEMTVSYLKTKKQSGPPTSSNSSDQVNPEKFRAGYTQAAYEVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT84</accession>
    <name>T1GT84_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Single domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006907-RA">
        <property type="protein sequence ID" value="MESCA006907-PA"/>
        <property type="gene ID" value="MESCA006907"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158197_0_0_1"/>
    <dbReference type="OMA" id="MPGCEEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029277">
        <property type="entry name" value="SVWC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053308">
        <property type="entry name" value="Vago-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957:SF1">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15430">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01318">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588488" description="Single domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Single" evidence="4">
        <location>
            <begin position="44"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006907-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14046" checksum="41F4B83BF1DF33BC" modified="2013-10-16" version="1">MCSKSNSFVAVLAFIVIMLTIKNEVHGAVFSQRAYIHPGHPGKCFNPLTYKAMLPDKEYKVKGVCAVMTCDIENLEIAIETCPVIDMPGCVEIPSDLSWSFPKCCPRYRCEDFKTGEPVIFDATSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ0</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
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        <lineage>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007089-RA">
        <property type="protein sequence ID" value="MESCA007089-PA"/>
        <property type="gene ID" value="MESCA007089"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2500494_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007089-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9758" checksum="39067855EF7A5D9D" modified="2013-10-16" version="1">MQLIEDQMYTKFGLGLNKYTIHKELDKDLKKKKLVTQPCALSSILSFGTFAFAFNTNLCYCIRPLVTMAIQEATGKNITMIMPFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTZ1</accession>
    <name>T1GTZ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA007190"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007190-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="24538" checksum="47E37C50EEB09652" modified="2013-10-16" version="1">YYADDTVGCEVFHYCQDNQRHSWVCPEGFTFHQVHLICMPPSHDNICEQSQKYHVVNNYLYKPINLEEHQSKPNVSLRYSERYFPEDIYEDQRYQYESRQDRERAQHHHQRQPVQVGFQHAQPQQPQPQQQIHQVQQPQRVTQPTRHNLVTNHPQQHQTHQQVFRSPEEINISLQQRRPQIFIATSTPRYYEDEYSYERRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU16</accession>
    <name>T1GU16_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Platypezoidea</taxon>
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            <taxon>Megaseliini</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="36166.T1GU16"/>
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        <property type="protein sequence ID" value="MESCA007216-PA"/>
        <property type="gene ID" value="MESCA007216"/>
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    <dbReference type="OMA" id="QIYHSAP"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588503" evidence="1">
        <location>
            <begin position="18"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007216-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="15368" checksum="36AAFBB445D6FD3E" modified="2013-10-16" version="1">MLRILQIFCLIVAATTASPIYDSHSYSAPILHSAPIIQSAPIIHSAPIIHSAPIIKAAPAATSYSSFNQVVHSAPVVKALPVVKYSAPIVSAPIVKYSAPIVSAPIVKYSAPIVSSPIVKYSAPISLGHSFDYGYGGYGGYGGYGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUI0</accession>
    <name>T1GUI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GUI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007393-RA">
        <property type="protein sequence ID" value="MESCA007393-PA"/>
        <property type="gene ID" value="MESCA007393"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_275107_0_0_1"/>
    <dbReference type="OMA" id="SWLFNDY"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20959">
        <property type="entry name" value="IgI_6_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000333">
        <property type="entry name" value="Down syndrome cell adhesion molecule"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000017">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF101">
        <property type="entry name" value="ZWEI IG DOMAIN PROTEIN ZIG-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="86"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="182"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="288"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="384"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="505"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="570"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="666"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="762"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="858"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="965"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1063"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1159"/>
            <end position="1249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007393-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1291" mass="139333" checksum="02CD8F100C83EFB6" modified="2013-10-16" version="1">PANFGDSATIVCSVLSGDLPIDIEWLFNDYPLNSYSDVSIMKGSKKNSMLTIESVNGRHAGNYTCIAKNSASTVSQSAELIVNVPPKIAPFDFGDDPANFGDSANVVCLVLSGDLPVDISWLFNDYPVNSYSGISIIKGSKKNSILTIESVTGRHAGNYTCTARNEANSVSYSAELIVNVPPKISPFHFGEEPANFGDSASVVCLVLSGDLPVDISWLFNDYPVNSYSGISTMKSSQKTSFLTIESVTGRHAGNYTCTARNEANSVSYSAKLIVMVRDIFHIYCFIPPKISPFDFGEEPANFGDSASVVCLVLSGDLPVDISWLFNDYPVNSYSGISIMKSSKKTSILTIESVTGRHAGNYTCTARNEANSVSYSSELIVNVGPKLAHFDFGEQPANFGDSISVVCSILSGDLPIDIEWLFNDYPINSYSGINVIKGGRKSSMLTIESITGRHAGNYTCTAKNSAANVAQSAQLVVNVGPKIGHFDFGEEPAISVILFLFGDLPIEIEWLFNDYKITSFSGINIIKGGKKSSVLTIESISGRHAGNYSCRATNEASSISHSAELIVNVPPKLQHFEFGDEPANFGDSASIMCLVLSGDTPIDIDWTFNDYPINSYLGVSVVKGGKKSSMLTIESVSGRHSGNYTCRARNSAATVSQSAILVVNVGPKLAPFDFGPEPANFGDSSSVTCLILSGDLPVEIDWLFNDYPINSYSGVTVVKGGKKISMLTIESVTGRNAGNYTCMAKNNAGAVSHSAQLIVNAPPKLAHFDFGEEPANFGDSVSIMCSILSGDLPIDIDWLFNDYPINSYSGITIAKMGKKASALAIEKVEGRHAGNYTCRAKNQADIVTHSSFLVVNALPKIAYFDFGTEPVNFEDSVSVTCVVSSGDLPVDITWFVNSAPVNLYNGVSTFKSGKRSSVLTIDSVHAGHVGNYTCRAQNPVGSDNHTAELNNLYLLLVFCILISSPPKITPFSNGEEPVHIGQYITYQCTVTEGDLPINILWTLNNHPIVVSDDDEIIISKLGKRSSVLTIENVSDKHAGKYTCQGENKAGKSSYSTELKVIVPPRIIPFAFEEGPAQTGQFITLQCTVPEGDLPLSIQWKLNGQLASVNLGITTTKVGQRSSLLTIDAIDEHHAGNYTCHAENRASSQNFTAVLMVNVPPKLAPLPTNEPLYPGDFFQLTCNVVHGDSPYNITWYFNDEPVGHIDGVNIMWASKRTSLLNIDSVRDIHDGNYTCVGSNSAGQSRVTSVLSVKGSFRISFVKHFNIKNLYLVIGGNNFVTQIKLLYVIEIISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV12</accession>
    <name>T1GV12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02137167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV12"/>
    <dbReference type="STRING" id="36166.T1GV12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007588-RA">
        <property type="protein sequence ID" value="MESCA007588-PA"/>
        <property type="gene ID" value="MESCA007588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_2_2_1"/>
    <dbReference type="OMA" id="MMENIED"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000450">
        <property type="entry name" value="PR domain zinc finger protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="457" mass="52155" checksum="12274DCF1D18D1E5" modified="2013-10-16" version="1">MVQIAGLQHASPNRLERVVKATKERDEIFIENNQLTMEIQFLPTLHGGLTITPITTSNNNNNDSPPEPKKKKIDHDLTFLCIGCGESYDHPFKLFTHLQTCEKLEDKEDDNEMKRRRDDDETEDNASLGFHSLKDVKSEDSEDSSSYTIELVKKNRRGRKRLSEVTNTIKMDTNCFQCTQCVESFNNAGDLSKHVRSHVNERPFMCSICQKTFTHIGSLNTHLRIHSGEKPYKCDLCPKAFTQSSSLMVHVKSHSVKKAHQCHLCEKGFTNYSSLVMHIKQHEEPTEVTCQQCDKVFKHEALLEEHMRLHASESLYQCAICCDSFKTANDLVQHMKNHIGDRPFQCSICEKSFTQPGSLNIHMRIHTGEKPHQCTVCDKTFAQASGLTLHQKQHEQFGCLICGSVCKDVEDLNQHILTKHMETKDGVQCLICKLVFKDKVDLASHVTNNHAEYLQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV40</accession>
    <name>T1GV40_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02160082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV40"/>
    <dbReference type="STRING" id="36166.T1GV40"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007622-RA">
        <property type="protein sequence ID" value="MESCA007622-PA"/>
        <property type="gene ID" value="MESCA007622"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_6_1"/>
    <dbReference type="OMA" id="FCICGRI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16267">
        <property type="entry name" value="HBS1-like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04093">
        <property type="entry name" value="HBS1_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037189">
        <property type="entry name" value="HBS1-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109732">
        <property type="entry name" value="HBS1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="244"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007622-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="653" mass="71977" checksum="459B9F32EEB37202" modified="2013-10-16" version="1">MSRHRNVRSMNYDEYDDEDVYGHSVDDDYSISPTDAQQWLYDRARGQNSMASFIANNDDIEEEDEEAENERFAKSRRDSDNFQMPKLDDVDQARLVSCIEEVRNVVGDEMVSERVIVETSMKFNYDITKVLDEILNECSSKTASAAASTVAAPIVTVPTATTVVPPPPTVKIITSVPVPVATTAVPAAKLFAQQKSPITSGRNTPEPQSLEQSSEDTLKTYKVSKEQAQRDAKQVFQSERGDEKAHFQMICIGHVDAGKSTLMGHLLVDMGNVSQKVMHKYEQESKKIGKQSFMYAWVLDETGEERARGITMDVGSSRIVTKSKIITLLDAPGHKDFIPNMISGATQADVALLVIDATRGEFESGFESGGQTREHAILVRSLGVNQLGVVINKLDTVNWSEDRFNEIVSKLKTFLKQAGYKESDVTYIPCSGLTGENLTKPASEPQLTSWYKGTFLLDVIENFKIPERAIDRPFRLSVSDLFKGTGSGVCVNGRIDTGMVGVNDKVLVGPNREQAQVKSISIDEISQNSAFAGDQVSMTLLGIDPNNISVGNLVILVFNVKVPITIGFPVILHYQSLVEPATIVKLTAQIHKGTGEVLKKKPRFLENNSCALLEIETTKSICIEKYGDFKELGRFMLRVAGVTIAAGVVTKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVD3</accession>
    <name>T1GVD3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007727-RA">
        <property type="protein sequence ID" value="MESCA007727-PA"/>
        <property type="gene ID" value="MESCA007727"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962112_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007727-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14834" checksum="91A2B0C2B754B87F" modified="2013-10-16" version="1">MEDLMMYDAWTQTDAPFCQQYDPISSSYASPTELKLMEMMEALQEQLSIQTSYIQSLQQNVNAVQRSTTTSSPTTTATTEPSNYRIITDGVLEPVEIEEINRELMQQLHKINHKRKPKYIQVKQRPMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVE4</accession>
    <name>T1GVE4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02154080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVE4"/>
    <dbReference type="STRING" id="36166.T1GVE4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007741-RA">
        <property type="protein sequence ID" value="MESCA007741-PA"/>
        <property type="gene ID" value="MESCA007741"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1103834_0_0_1"/>
    <dbReference type="OMA" id="GGHITNC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007741-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="27112" checksum="916FBA26D9EFAC01" modified="2013-10-16" version="1">MRLTGNEPRKDKTDAPNGGKHNLSPNTEQPCQGSILPPPGGGNHLDGSQQNGNKERPTNLGGHITNCQGGLFAIIMNIVIENVGQPGSTPPPYPQQAGDGNHRPGHMVLSELPEPPIPLSEIGPIPPPPMFSTPSPTLVAGKAHGRGAVNDKDYQGYDYDEQDGDNEELESDEEYLYTIAQPNPNIDTMRVEEIPAKEPKMNAVPLKSALKKRPGQVASNSSPATPTQDHNSSTSQRPLVVRQQESNNYSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW04</accession>
    <name>T1GW04_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW04"/>
    <dbReference type="STRING" id="36166.T1GW04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007972-RA">
        <property type="protein sequence ID" value="MESCA007972-PA"/>
        <property type="gene ID" value="MESCA007972"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248071_0_0_1"/>
    <dbReference type="OMA" id="QRIMITH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007972-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="222" mass="24947" checksum="2C2F776707F4BDCF" modified="2013-10-16" version="1">MPRIKQCFKCQRFGHLAVNCKSSADICAQCGQNHQSNNCRSNCIKCPNCGENHPSTSKDCSARAEYAEIQRIMITHNLNFLEAKNRLRPQFQLDPIHFPPLTKGFFKAAIAPSAPGPADAPITVAQQVQLSSKPKPNKRNKNKPKTKKALSPSVTEITETKDEEPFEGFEEETMEEQSAESDIEFDDVSTSSKRQRLSEAIKDKREQEMNNKSINSSFELNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWM9</accession>
    <name>T1GWM9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008207-RA">
        <property type="protein sequence ID" value="MESCA008207-PA"/>
        <property type="gene ID" value="MESCA008207"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_890195_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008207-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="313" mass="36301" checksum="E28E0F4ECCA9C5A5" modified="2013-10-16" version="1">MDLNTNFNPLSEMCSKFLPNSISSKMKSDSQQFSFPKTNPLSEKIVFKFSEPIAIKIEKDESPNPEPSAFLQETTTISRLKILRATFKEQENHDSISKVQVHSSVCEVSESYSRKYRQRSKSSDYSDSKNSRNHRKASKSEKHRSRRNSRSGSINLSDSYSVSSRNRMPNYSDYEKSRNNKESLRTKNAKVEEITDQGHLIIQKLLEMGKNHLRNLEEATDRDLDLLIIRAHKIIEILKILKIRLYQSCYVPGKENLEGLLRRVLKELVIQDQILKLIIILQESKGLQKILERETASNKNGEILIIRNIKMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXI4</accession>
    <name>T1GXI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008536-RA">
        <property type="protein sequence ID" value="MESCA008536-PA"/>
        <property type="gene ID" value="MESCA008536"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078924_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008536-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13305" checksum="1A2841B81DFF4DDF" modified="2013-10-16" version="1">MIENCKKRWLSIDSCVMSKKVWGDINAKASKSLLSLRRNSHRKIIFDVTGHWPIGIQGKRMKIPVSEFCESCGQAAECITPENFWFACPGLSDKIDAKASKSLLSLSRNSLRKMIFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY18</accession>
    <name>T1GY18_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY18"/>
    <dbReference type="STRING" id="36166.T1GY18"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008738-RA">
        <property type="protein sequence ID" value="MESCA008738-PA"/>
        <property type="gene ID" value="MESCA008738"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592487_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019321">
        <property type="entry name" value="Nucleoporin_Nup88"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10168">
        <property type="entry name" value="Nup88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008738-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9715" checksum="06F89CF16276CE71" modified="2013-10-16" version="1">MFNYVRECLRDTIPINKSINLIEIIDDLLFFWNVKDCCLHVINWRALKSLNKDSIKYQEQNTSVNSLEFLYFEVQTYEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY24</accession>
    <name>T1GY24_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02064896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY24"/>
    <dbReference type="STRING" id="36166.T1GY24"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008744-RA">
        <property type="protein sequence ID" value="MESCA008744-PA"/>
        <property type="gene ID" value="MESCA008744"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375151_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008744-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10821" checksum="6DFC0C72B9B1559B" modified="2013-10-16" version="1">MTSSGNLACNKEDFNISRSKLCLVHTNKSICGEVDFIPTKFLRNKTEEQEHRDNYVTAVSVFIIEVGPDTKEMLKLLEFEDVKSLNPPPKITTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYD9</accession>
    <name>T1GYD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hexosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02372805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYD9"/>
    <dbReference type="STRING" id="36166.T1GYD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008864-RA">
        <property type="protein sequence ID" value="MESCA008864-PA"/>
        <property type="gene ID" value="MESCA008864"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1251915_0_0_1"/>
    <dbReference type="OMA" id="DWFWANI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035248">
        <property type="term" value="F:alpha-1,4-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051981">
        <property type="entry name" value="Glycosyltransf_32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12042:SF21">
        <property type="entry name" value="ALPHA1,4-GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12042">
        <property type="entry name" value="LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008864-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25592" checksum="5A41972E77644755" modified="2013-10-16" version="1">MNNKQIIKNISKPRTWMQCIIVLGIVSFLVIHTLMPMVYNQMKLSKIIHPNNLIMPNQFRPRASSKYLGKNEYMNCLIFPTIKSNSSIFLPDIMDSEVPPVKNSIFFHETSCSKDHTQDLDFLQACAIESAADANPNTEIYVLFASPRLDISNKSYALMGLLDSFGNVHFRNNDIWKYSKKTPARKWLSKGYIFQSEYVKDQLQSFLAYLSLWKWEGTFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYI6</accession>
    <name>T1GYI6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02075785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075786">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYI6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008915-RA">
        <property type="protein sequence ID" value="MESCA008915-PA"/>
        <property type="gene ID" value="MESCA008915"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913895_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008915-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="305" mass="34161" checksum="646C7732665AEBF2" modified="2013-10-16" version="1">MSFATDNHKGGKITPPLGVPLYTNFCIIRNLSDEEIKDNILRTLRTSDRFQSGKEIKKNISNWGLRKYLPPPIQIVIFDSSVKITTQYLKENTLVNLLTEEEGSDKIVLKKIAENVIYNPSDKNDNFSNSMESTSTSSAASSSTSTNSTLRKRKLNSSVTSRSSEEPCPSTSSGTIDDDDVSVEDYQSLSSRRKTGPLRILEELIPRTPSFNGIHNPFRIPSPKKIDPNSKAVKSKLQRIDLSTKLAALKKSGVIIKKRQNLTAGQLKGEILIKKRKLDEKDIIVTADKKVIKRKRGRKNKNETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYK9</accession>
    <name>T1GYK9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYK9"/>
    <dbReference type="STRING" id="36166.T1GYK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008941-RA">
        <property type="protein sequence ID" value="MESCA008941-PA"/>
        <property type="gene ID" value="MESCA008941"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152078_0_0_1"/>
    <dbReference type="OMA" id="IKGNTWI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008332">
        <property type="term" value="F:low voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070509">
        <property type="term" value="P:calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF230">
        <property type="entry name" value="CA[2+]-CHANNEL PROTEIN ALPHA[[1]] SUBUNIT T, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008941-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12744" checksum="3DC8C7963E06916D" modified="2013-10-16" version="1">VFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGDNLKSVRNKTDCLAIKGNTWINRKYNFDDLGKALMSLFVLSSRDGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYR1</accession>
    <name>T1GYR1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189448">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYR1"/>
    <dbReference type="STRING" id="36166.T1GYR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008998-RA">
        <property type="protein sequence ID" value="MESCA008998-PA"/>
        <property type="gene ID" value="MESCA008998"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023330_0_1_1"/>
    <dbReference type="OMA" id="KPFQYMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19598">
        <property type="entry name" value="serpin77Ba-like_insects"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF367">
        <property type="entry name" value="GH21475P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577755" description="Serpin domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="59"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008998-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="413" mass="46502" checksum="1438B8F2DCDB21F1" modified="2013-10-16" version="1">MSLSKDKNFIAYLCVILVITQCLCQQRNILQTVVQRIDAPPEIQRALKSISEGSEQFALELFTKAATALKDENSHYGDFMISPFSVWSLLVLMVEGASGNTLAELQKTLRINTDLTAMRTAYERINQALRVNTTTLQVSTLQAIFSDINRPVDRDYEDVVERIYRANLVPINFHNSKESANIVNDFVNRETRGLIKQLVYQEDLSEAQMILISAIFFRGQWTMQDDVPERIFFYAAIPQLDSHVIELPYGVENRLSMIVVVPKKGVRLYSVIEKLASVGTSGIFNGLKKAKEDAEYYDEEMEVDLFLPRFTTDTDFALNSLLIQMGIHDLFNAKNANLGKISSHPIYLSRLYHKTTIEVTEEGTVASAATAGILTNKISPTKFQVNRPFAYMIVEKSTGSMLFVGQVKNPGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYV3</accession>
    <name>T1GYV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GHMP kinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02380947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYV3"/>
    <dbReference type="STRING" id="36166.T1GYV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009040-RA">
        <property type="protein sequence ID" value="MESCA009040-PA"/>
        <property type="gene ID" value="MESCA009040"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_3_0_1"/>
    <dbReference type="OMA" id="CISVEFP"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="5">
        <location>
            <begin position="106"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009040-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="235" mass="25850" checksum="75A515E83B4E5987" modified="2013-10-16" version="1">MLLNCRISAPGKVILHGEHSVVFGKDALVSSIGLRTQLYLLEKENTSCISVEFPNLQFKIETTLDDVNIFLESSKNTPELIRSFIMQRFKRSNEIENNTLVAFFFVLSKAFGQQKVKTAFNISLTSDLTIGAGSGSSAAFGVCIATAFLLLARKITDPYFEHLDLISNLAFESEKIMHLSPSGVDNTICTYGGILKFAKGQGFSKISIPKQNKLNVLLVDSGVSRNTANLVAHYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYZ0</accession>
    <name>T1GYZ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor TFIID component TAF4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYZ0"/>
    <dbReference type="STRING" id="36166.T1GYZ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009078-RA">
        <property type="protein sequence ID" value="MESCA009078-PA"/>
        <property type="gene ID" value="MESCA009078"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_683885_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08045">
        <property type="entry name" value="HFD_TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000015">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045144">
        <property type="entry name" value="TAF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138:SF14">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID component TAF4 C-terminal" evidence="7">
        <location>
            <begin position="214"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="303"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009078-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="403" mass="44460" checksum="E2B6D8E63BE2E1A2" modified="2013-10-16" version="1">MISNVQRPGQTTITKQISGGIRLQQNTSQQNLQIVKNNVTQSIRTTGSTIIRPPNPSIKSSSTQLVKNSTSAHTKVGQTQIKPTLVNHQLKIQSGSIQHNQNTTPSSITQQTSTATVTIPVNSNLHPTNLSNLNNSSPNINNTSTVTNASTNKIVTIKAQVNQNFTGKIIGKKKHNFQSSSSQFSHIDDKNVHTNSSGSSSFFQPSSMYGDDDINDVAAMGGVNLAEESQKILGSTENIGTQIRSCKDEVFLHLPTLQSKLKAVCNENGIDEPSLDVSVLISHACQERLKNIVEKLAVIAEHRMDILKELDKAEQKRHEEQEREILLRAAKSRSRLEDPEQAKLKAKAKEMQRAEMEELRQRDANMTALQAIGPRKKPRFDNESNPTISSIVSKLLIFVLCNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ71</accession>
    <name>T1GZ71_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inositol 1,4,5-trisphosphate/ryanodine receptor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ71"/>
    <dbReference type="STRING" id="36166.T1GZ71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009169-RA">
        <property type="protein sequence ID" value="MESCA009169-PA"/>
        <property type="gene ID" value="MESCA009169"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177893_1_0_1"/>
    <dbReference type="OMA" id="CTFVIEQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005790">
        <property type="term" value="C:smooth endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005219">
        <property type="term" value="F:ryanodine-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014808">
        <property type="term" value="P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006941">
        <property type="term" value="P:striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399">
        <property type="entry name" value="B30.2/SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399:SF8">
        <property type="entry name" value="B30.2_SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009169-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="51" mass="5531" checksum="B2345CCB1C9C3E46" modified="2013-10-16" version="1">MVCLSCTATGERVCLAAEGFGNRHCYLENIAEKNIPPDLAQCTFVIEQALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZA4</accession>
    <name>T1GZA4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PI3K/PI4K catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02025716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02025719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GZA4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009202-RA">
        <property type="protein sequence ID" value="MESCA009202-PA"/>
        <property type="gene ID" value="MESCA009202"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_792966_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="4">
        <location>
            <begin position="196"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009202-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="350" mass="39732" checksum="F17271DEDEE76731" modified="2013-10-16" version="1">MKLFTREMVKAASLAYASETDYQKDNNIVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNALENIAQFIQGESEMMELLVRLLELFVQIGLEDALCQNIIASFSGSAKQFYEREFEFFGKITDVSGKIRPFPKGIERKEACLRELQKIKVRAGCYLPSNPESMVLDIDYNSGIPMQSAAKAPYLAKFKVYRCGITDLEQIAMSISQSNVPKRVISQETWQAAIFKVGDDVRQDMLALQVITIFKNIFKQVGLDLFLFPYRVVATAPGLCVQAFLAVRPYQDAIISLVSLMLDTGLPCFRGQTINLLKQRFVANKSNKEAAVHMLSVVRNSYQNIRTRTYDMIQYYQNQIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZN8</accession>
    <name>T1GZN8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZN8"/>
    <dbReference type="STRING" id="36166.T1GZN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009342-RA">
        <property type="protein sequence ID" value="MESCA009342-PA"/>
        <property type="gene ID" value="MESCA009342"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399483_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016527">
        <property type="entry name" value="ORC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577264" description="ATPase AAA-type core domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="54"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009342-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="22593" checksum="30CED653F5EA5A10" modified="2013-10-16" version="1">MGIEQAGLHLFRCLLINSALYKLLADKSFYNNTIIVVLDGNIHTDERLALKSATVQMKLENAVDGKVFGSFAENLSFLLASLKSGNKKSKSVIFLLHEFDLFCSHHKQTLLYNLFDISQSAQAPICVVGITRRLDVIELLEKRVKSRFSHRQVFIFPREEEDIDSRMLRFKEIMTLMNEKERKQFYENKPNLKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZY1</accession>
    <name>T1GZY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02117851">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZY1"/>
    <dbReference type="STRING" id="36166.T1GZY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009442-RA">
        <property type="protein sequence ID" value="MESCA009442-PA"/>
        <property type="gene ID" value="MESCA009442"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099304_0_0_1"/>
    <dbReference type="OMA" id="IMLFMYY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004989">
        <property type="term" value="F:octopamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002002">
        <property type="entry name" value="Octopmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF174">
        <property type="entry name" value="TYRAMINE_OCTOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00664">
        <property type="entry name" value="OCTOPAMINER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="84"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009442-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="27078" checksum="3E82BE93AAEEFCFD" modified="2013-10-16" version="1">MYNTTIAHSIINRTTASTLPTLPTTSSVSSVATTSTYNVNYTSCAQVEPILYPSYFGYYLAVPEWEAILTAIVLSVIIIATIVGNILVILSVFTYKPLRIVQNFFIVSLAVADLTVATLVLPFNVAYSIIGKWVFGIHLCKMWLTCDVLCCTSSILNLCAIALDRYWAITDPINYAQKRTVGRVLFLIAGVWILSLIISSPPLVGWNDWPDDFTSDSPCELTSNRGYVIYSSLGSFYIPLIIMTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H062</accession>
    <name>T1H062_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H062"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009530-RA">
        <property type="protein sequence ID" value="MESCA009530-PA"/>
        <property type="gene ID" value="MESCA009530"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923098_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009530-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="302" mass="32237" checksum="8220B1A9D041FB66" modified="2013-10-16" version="1">KISASRTTTTATTATTTTRPPNNHEEHFYNKNNCKINTTTPLNNTFDYDFIDKHLSQLDLSSSLSSFNQSNHVLQFLSSPNSSIQNLSKNLSSPNQNQNQIQNQNHNNSQLFPNTSPSSTSSSSSASSSSSSLYSGNNILSNNNIINHNNNNNNNYNCTAGAEEVSAAVIPTTSQSMDNNNLCSTQGTSSTTGTTMNQQQQQQQQQSQRPTDFVGNSFGIGSGLTSPNSSGGILTGLNSISNSLKSQIFQEQLSPNSSSQSSNITTATNNMKVGSSGSPISTNSSPISTPTSPNSQQMQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0E9</accession>
    <name>T1H0E9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02064601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0E9"/>
    <dbReference type="STRING" id="36166.T1H0E9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009625-RA">
        <property type="protein sequence ID" value="MESCA009625-PA"/>
        <property type="gene ID" value="MESCA009625"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860234_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009625-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="16118" checksum="BEF071692D3C125D" modified="2013-10-16" version="1">MISPGGISPRFKCRKKNSKIDKEPIYKCHKCGKKYRWKSTLRRHEHFECGDKAPLHSCPYCSYKSKQRGNLGVHVRKHHGHLPQLENLRRRSNSGAATSSNSLNRYESDDDERDEDDDDYEEEEENFSSDDNESSDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0T6</accession>
    <name>T1H0T6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0T6"/>
    <dbReference type="STRING" id="36166.T1H0T6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009777-RA">
        <property type="protein sequence ID" value="MESCA009777-PA"/>
        <property type="gene ID" value="MESCA009777"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068978_2_0_1"/>
    <dbReference type="OMA" id="HRCTTEH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF3">
        <property type="entry name" value="FURIN-LIKE PROTEASE 1, ISOFORMS 1_1-X_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="6">
        <location>
            <begin position="4"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009777-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="17098" checksum="4C8B0FB7EC30FB77" modified="2013-10-16" version="1">MPSDPKASFDVNNNDSDPSPQYDMTDSNRHGTRCAGEVAATANNTFCAVGIAYDAKVGGVRMLDGDVTDAVEAKSLSLNPQHIDIYSASWGPDDDGKTVDGPGDLATRAFIEGVTKGRNGKGSIFIWASGNGGRELDNCNCDGYTNSIWTLSISSATEDGNVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H243</accession>
    <name>T1H243_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00929"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="174" mass="20119" checksum="864AEAD22CCA5D8D" modified="2013-10-16" version="1">MNLKTGKIEAEFHKYVMPIESPKLSSFCTELTGITQNKVDTGIPLQTALMVFQEWVRKELRSRNLYLPKMNKDKQSGNVAFVTWSDWDFETKEPPFFNQWIDLKAIFKEYYHHKPINFNDALLHVGLQFAGREHSGIDDSKNLASLVYKMVKDGATLVITKDLTPFQLTINFSL</sequence>
</entry>
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                <person name="Hughes D."/>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="19815" checksum="7C3106953BC6BDC7" modified="2013-10-16" version="1">MLECMAKCKGVYQAIKFYENVNRQRKRHHQHQHPVFSSSNGGASKTSSDFSLGISSTDWTGFFPFFFFPASGTDSAALALFLPFFDCSGALTNGSACATFLIFLGTGTSSSTGFLVFLGFFTGASDLTSSLTTKAFFSFLAGTVFSGAESSFGVAFSGTFLALFFLAALPFCGGAISLISSDLLVQN</sequence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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        </source>
    </evidence>
    <sequence length="99" mass="11654" checksum="F5E1EF92CBA3D1A7" modified="2013-10-16" version="1">MVLLLAPFNNIHVNESKITKEIWEALEKAFNDSGLMRRVSLIRKMINTKLEDYSTVQERDWIADFQLFKASIDEEVTVANKSTIAVEQVTVYYTNERRW</sequence>
</entry>
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    <accession>T1H3R3</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="16640" checksum="3BFCC28E89B9E33C" modified="2013-10-16" version="1">TTTTKITTTTIPTTTVESTTTTLTTTTTLPTTSVESTTTTITTTVEIPTTTTELTTTIITTTTIPTTTTTTVEPTTTTVTTTVEIPTTTTELTTTTTVLITTTTVLITTTTELITTTTELTTTTITTSTTTTTTTTTTTMAPVTCKSTYLDLEEHPDL</sequence>
</entry>
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            <taxon>Neoptera</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <authorList>
                <person name="Hughes D."/>
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            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <name>Zn(2+)</name>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="34"/>
            <end position="162"/>
        </location>
    </feature>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010918-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="20845" checksum="829D0D0ABDE90B69" modified="2013-10-16" version="1">MSTAGKVIKCRAAVAWEPKKPLSIEEIEVQPPKAHEVRIKLTATGVCHTDAYTLGGFDAEGVFPVVLGHEGAGIVESVGEGVTDFKVGDHVIPLYIPQCKKCEYCLNKKTNLCQSVRTTQGKGVMPDGTTRFSCKGQPIYHFMGCSTFSEYTVVADISVAKIDEKAPLDKVCLIGCGIPTGYGAALNTAGSSLFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3X4</accession>
    <name>T1H3X4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02193839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3X4"/>
    <dbReference type="STRING" id="36166.T1H3X4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010971-RA">
        <property type="protein sequence ID" value="MESCA010971-PA"/>
        <property type="gene ID" value="MESCA010971"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032862_2_1_1"/>
    <dbReference type="OMA" id="HIMLITR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000051">
        <property type="entry name" value="Acyl-coa thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="83"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010971-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="311" mass="35494" checksum="8E665E7211448688" modified="2013-10-16" version="1">FQKIIQPKSLKIFQYKSFSNDIPRPENLATAGTMSDVYEKILKRLGAEPGYREVPQSREHLLQYQPAAEDLPARTMADSFASAIIPLSSSKNLQDKYVNWLGYARLGKLIQDMDGFAVWVCQQHLLIPNLDESIHLPYTFVTILVDRIDFDAFRPKGTTDIRLSGHVSWVGRSSMEVVVWLEQEFEGKYKKITRALFLMAARNATNTGPAPINPLVPGNDKEKEILAGGEKRKQLRLESQNQSLLKVVPNSYEQALMHDLFIRTTSKETIQLNKRMLPDNSKWMKHCSNSNTIPCFSEYRNAQKPFSEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Z8</accession>
    <name>T1H3Z8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrator complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Integrator subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02381913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Z8"/>
    <dbReference type="STRING" id="36166.T1H3Z8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010995-RA">
        <property type="protein sequence ID" value="MESCA010995-PA"/>
        <property type="gene ID" value="MESCA010995"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_393261_0_0_1"/>
    <dbReference type="OMA" id="TDECINI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026236">
        <property type="entry name" value="Int2_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029321">
        <property type="entry name" value="INTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28608">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28608:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14750">
        <property type="entry name" value="INTS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02105">
        <property type="entry name" value="INTSUBUNIT2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010995-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="81319" checksum="01009F829EE63785" modified="2013-10-16" version="1">MKKFTAIEWMQWLIKEEKYFEKKIGNEGSLGELLLLIAIHFHSNQISAIAELICSNLDMKIPIRPNNTTRIKQLFIQDEQIVAAHAVKVPVTINLNGNIPGYLPVHCINQLLKSRSFLKHKVTIKSWIYKQICSCTTPLHPVMPTLIEAFVNSLILPGKLVSGGGSSGNIVDHLHKPLSEDEILKVFQQKNDQGDKSLNSSIYSDISKQSNLTSQVLLLYYLVLYEEVRQSNMVTIIQTNRNIKKYSVEFMSELPIKYLIQQVQLKQDQYTSLFHPMLKLLITHFPHISLVDDWIDEDKILNVKFCKKKDFNESQLLDAFENLKFNPSKLMNILVEMTKLSPTELWKFSDVLVKNFNTILTNNVPKRTQELYKKLKFCLTEKTLVNDNYNYLPKEFIPLTILKYSNENVLSDPLQILKCKDDIFRSPDGLSLILRILQSSLAASKIQLMRHIQEKPLFDKDGQFQNEEEREQLKTSLIASQESAAVHILLESCLSSNSELCNFGKLWALREIRGIICSYIHQAFISEPSLANIVHFQTYDNKLLKISVNGIPSMHICIDFLAEILNIPEMIKQVFTIDLTSHLALKYAIPKGLSTSKLCLNTFSTLIGLLTTDIRIEMFSFMLPSIPRFANAFPVLTDECINILMQLGKILYTQYALGRNSFSVPSLALHSSSKIKAKIAYSRYSYDLFLEVKLTFLKIVTNTINNISMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H424</accession>
    <name>T1H424_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H424"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011023-RA">
        <property type="protein sequence ID" value="MESCA011023-PA"/>
        <property type="gene ID" value="MESCA011023"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725068_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011023-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8259" checksum="727999EA0A53A947" modified="2013-10-16" version="1">MLDKTNEVRAGATRVPMQSASLYCRSLFLIALDKAEHQVCRARSLVCRASAFHTKFQRPKLIYIKLIFRTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H473</accession>
    <name>T1H473_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003031">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003032">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003035">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H473"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011076-RA">
        <property type="protein sequence ID" value="MESCA011076-PA"/>
        <property type="gene ID" value="MESCA011076"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375275_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011076-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="23293" checksum="0660ED3510945472" modified="2013-10-16" version="1">QHLNSKSFDNALCNSNVNLREASLIDLNVNFTEKSNNKSSENYKNVQENVDENLCKINYECSNLERLKTYNEARARIFSKIPPECTNKYKRLIGFHGTKQPVIGVVNLLVKYNSIEKTHKFFIVPSLSQELYLGFDFWRCFNIAPELHCLEVSEINFDLDIPKVYPENDQHILNPEQSFRLEKNKNFDLNHDSFSIQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H536</accession>
    <name>T1H536_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H536"/>
    <dbReference type="STRING" id="36166.T1H536"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011410-RA">
        <property type="protein sequence ID" value="MESCA011410-PA"/>
        <property type="gene ID" value="MESCA011410"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3225151_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011410-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="44" mass="4788" checksum="FE091006EEDB4089" modified="2013-10-16" version="1">MTALLRHYHPTVRRIAMNIVNGVPASGEGSLPAEIGKLLVFLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5B7</accession>
    <name>T1H5B7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02163819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5B7"/>
    <dbReference type="STRING" id="36166.T1H5B7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011494-RA">
        <property type="protein sequence ID" value="MESCA011494-PA"/>
        <property type="gene ID" value="MESCA011494"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043453_1_0_1"/>
    <dbReference type="OMA" id="QKSTRIY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011494-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="22181" checksum="F42EFB6DD828BE18" modified="2013-10-16" version="1">RWRAAKEAETGEKDRRPFLATQCTELPKCEKYRLEIIREISKKVAQIQNAGLGEFRIRDLNDEINKLLREKRHWENQISSLGGPHYRRYGPKMFDAEGREVPGNRGYKYFGAAKNLPGVRELFEQETQYVPKKNYAELMKNIDAEYFGFQDDDDGILIPLEEKAEKEYREKWISEWKEKNGLEEMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5I8</accession>
    <name>T1H5I8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5I8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011566-RA">
        <property type="protein sequence ID" value="MESCA011566-PA"/>
        <property type="gene ID" value="MESCA011566"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3428615_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011566-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="20" mass="2128" checksum="9219BB6E00E080FA" modified="2013-10-16" version="1">MSFFVGTIFAYSWKASSGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7L4</accession>
    <name>T1H7L4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H7L4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012751-RA">
        <property type="protein sequence ID" value="MESCA012751-PA"/>
        <property type="gene ID" value="MESCA012751"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_395167_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF28">
        <property type="entry name" value="NANCHUNG, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="548"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012751-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="698" mass="79054" checksum="45E05E50B80F5CF2" modified="2013-10-16" version="1">MGNTESNVTSGVKKQAGVSTQGLYKLVDLKGGGLLVDMMKRACQNKQFAEIDHAIKTKVEPYLYNKGHGKYIPISKIVLLRNKDRPRHKQLPEIRALENPEDDFNIDDFCPEVTEEQYKSNPNLYRLVCWNLNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFVGAIFYLLAALMEAKFLGYKMFIENLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAYYIIFLTFEDEEDEEGNPMSSPMESMVAMFLMSLTNFGDYYAVMVSTDHSVEAKILFVLFMVIVAVLLVNMLIAMMGNTYQKIAETRNEWQRQWARIVLVVERGVPPKERLRNFNEYSQPMSDGRPSLVLRLNMSDDKHEMKEVLEMKRIH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>O01671</accession>
    <accession>O96931</accession>
    <accession>Q9GVB9</accession>
    <accession>Q9U536</accession>
    <accession>Q9U537</accession>
    <accession>Q9U5M7</accession>
    <accession>Q9U5M8</accession>
    <name>SXL_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName>Sex-lethal homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">SXL</name>
        <name type="synonym">SXL2</name>
    </gene>
    <organism>
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="382" last="390">
            <title>Sequence conservation and expression of the sex-lethal homologue in the fly Megaselia scalaris.</title>
            <authorList>
                <person name="Sievert V."/>
                <person name="Kuhn S."/>
                <person name="Paululat A."/>
                <person name="Traut W."/>
            </authorList>
            <dbReference type="PubMed" id="10791828"/>
            <dbReference type="DOI" id="10.1139/gen-43-2-382"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2; 3 AND 4)</scope>
        <scope>TISSUE SPECIFICITY</scope>
        <source>
            <strain>Wien</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Unknown; apparently not involved in somatic sex determination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>O01671-1</id>
            <name>1</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>O01671-2</id>
            <name>2</name>
            <sequence type="described" ref="VSP_007188 VSP_007190"/>
        </isoform>
        <isoform>
            <id>O01671-3</id>
            <name>3</name>
            <sequence type="described" ref="VSP_007189"/>
        </isoform>
        <isoform>
            <id>O01671-4</id>
            <name>4</name>
            <sequence type="described" ref="VSP_007190"/>
        </isoform>
        <text>Additional isoforms seem to exist. The event occurs in a sex-independent manner.</text>
    </comment>
    <comment type="tissue specificity">
        <text evidence="3">Expressed in gonads and somatic tissues of both sexes. In the ovary, expressed in the last egg chamber of each ovariole. Highly expressed in nurse cells with low expression found in oocytes. Highly expressed in testis with lower expression in testis sheath and vas deferentia.</text>
    </comment>
    <dbReference type="EMBL" id="AF110846">
        <property type="protein sequence ID" value="AAF15091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF110846">
        <property type="protein sequence ID" value="AAF15092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ000546">
        <property type="protein sequence ID" value="CAA04179.2"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ245662">
        <property type="protein sequence ID" value="CAC01696.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X98769">
        <property type="protein sequence ID" value="CAB61832.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X98770">
        <property type="protein sequence ID" value="CAB61830.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X98771">
        <property type="protein sequence ID" value="CAB61831.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O01671"/>
    <dbReference type="SMR" id="O01671"/>
    <dbReference type="STRING" id="36166.O01671"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050686">
        <property type="term" value="P:negative regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007530">
        <property type="term" value="P:sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000205">
        <property type="entry name" value="Sex lethal, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000383">
        <property type="entry name" value="Sex lethal, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006546">
        <property type="entry name" value="Sxl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01659">
        <property type="entry name" value="sex-lethal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000081969" description="Sex-lethal homolog">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="RRM 1" evidence="2">
        <location>
            <begin position="78"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="RRM 2" evidence="2">
        <location>
            <begin position="164"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_007189" description="In isoform 3." evidence="4">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_007188" description="In isoform 2." evidence="4">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_007190" description="In isoform 2 and isoform 4." evidence="4">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O61374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10791828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="321" mass="35738" checksum="E2F18FC8AA0B68C0" modified="2003-04-23" version="3">MYRGGRMWGMSHSLPSGMSSYAFSPQDTDFSSYPSTIGRQHSQQSQRYYQNNNCGLGSVGNMANSTNSLNSGTNNSGTNLIVNYLPQDMQDRELYSLFRTIGPINTCRIMRDYKTGYSYGYGFVDFGSEADALRAINNLNGITVRNKRIKVSFARPGGEQLRDTNLYVTNLSRSITDEQLETIFGKYGQIVQKNILRDKHTGTPRGVAFIRFNKREEAQEAISALNNVIPEGGTQPLTVRVAEEHGKSKGHVYMAPNQPPHGNMGHGNMGNMGHGNMGMAGGSGMNLNNMNAFNGMNQMVHRGRQKHSYQRKIHPYNPNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9W5</accession>
    <name>T1G9W5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9W5"/>
    <dbReference type="STRING" id="36166.T1G9W5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000006-RA">
        <property type="protein sequence ID" value="MESCA000006-PA"/>
        <property type="gene ID" value="MESCA000006"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153430_0_0_1"/>
    <dbReference type="OMA" id="NTQVMKS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000006-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15821" checksum="52240CC839078469" modified="2013-10-16" version="1">MVQRLQDKEDDVKKAFYEQLQQAYGRLPNFFKKLVLGNFNTQVMKSIVCLLFVAVFAVAIALPQREGASYTNEAIRQAQQTLLIPRDAQIQKVEEGIELGAYEQIPGNQRINLFEILGDQVPSEVIKNLQQQVDQIGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAE7</accession>
    <name>T1GAE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DOCKER domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02037832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAE7"/>
    <dbReference type="STRING" id="36166.T1GAE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000202-RA">
        <property type="protein sequence ID" value="MESCA000202-PA"/>
        <property type="gene ID" value="MESCA000202"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000624_3_0_1"/>
    <dbReference type="OMA" id="PIASHEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF26">
        <property type="entry name" value="ZIZIMIN, ISOFORM K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOCKER" evidence="4">
        <location>
            <begin position="698"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000202-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1131" mass="129636" checksum="F2D054B92FD19963" modified="2013-10-16" version="1">MSAMPAESETCKILKAAHAIQISSLISFLPTILNQLFTLLVNTTSEEIGMNVIRLLINIIHLLTEAADRKDLLLSYVKLPDCNKFMKYSAIFFDIIVKSMAQHLLDSGRIKMHRNERFPKEFHDKIESLFKVFVPYLIHKYKDLPEETQELNKSICHFIKRSLTLMDRGFVFKMVRFYLQYFGPGDSRVLHEFKFQFIRELCFHEHYIPLNMPFAVNPKKQKKRNTLIQHFTLSEEFCREHYLAGLLLMEVKSSLNEVSNIRRMALGVLRELIAKHDLDDRYQNKGQLSRIALLYIPWMSVVLDNIHRIPDGTEVCKVNSHDNASLIHRMSTSSSFIFPTPTNSTPNKIKNRQTLHIEHPSPLRSSMHVRDSSYFAAIAGQIGNTNGASTCSLNSFSSDMSQEHHALNGDGGDGGEQMYIWWDNCSDSEINAFMIVLEICLYHFRYVGKINLVVSESKDFSRQAKNSKASTLPARMAPPAMENGAETNGSGTLNLTSNQRENLADEAVKNNQAMYESNLATEVGMVILDVLGWFSLKFHTAYTSARCDPNDTLKLGSVYLKFLQHGQSEHLSKHVFAALRSFINNFSLGIFKGHAVLCGQIVYELLKACDSHLVAIRHEACAVLYLLMRTNFEFSKRKGLTRVHLQVIISVSQMIGNVIGLNNARFQESLSMINTYAFSDKAMKGTGEAARAQYSLANSYASTPELRHTWLLTMARNHEQNGNISEAAMCYLHIAALMAEYLKLKGTIKWGAEAFKGISPNIARDEIGLKLDSGAQDSQYTEQMLLDQLKQCAEFLDKSERFECLGELYRLIVPIYEKRRDYYSLMSSHQHLSQVYTKIIEVNRSGKRMLGRFYRVVFYGQAYFEEDHGIEYVYKEPKLTTLSEISERLFKQYKDKFGNDLVKMIMDSSPVNVNDLDPKIAYIQVTHVIPYFCKDELDNRQNEFEQNHDVDRFVFERPYFEGPSPSGKVEDQWKAKTIIRTSYSFPYVLKRIPVKESQNSVLTPIEVAIEEMQNKVTELQEVILPPADVKKLQLRLQGSIAATVHSGPFAYAKAFLDPKNKDKIIDELRLVKIEDLKQTFRDFMEVSWTALKINERLISNDQKEYHSALHENYIKLSRELGELLNESFIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAJ5</accession>
    <name>T1GAJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nucleoside-diphosphate kinase</fullName>
            <ecNumber evidence="3">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02009365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAJ5"/>
    <dbReference type="STRING" id="36166.T1GAJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000252-RA">
        <property type="protein sequence ID" value="MESCA000252-PA"/>
        <property type="gene ID" value="MESCA000252"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640952_0_0_1"/>
    <dbReference type="OMA" id="IRAFIMV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="6">
        <location>
            <begin position="21"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000252-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8655" checksum="F5DD31F1BFD98AFD" modified="2013-10-16" version="1">MAINTLCVYFGANSNNCQPIRAFIMVITGGVQRGLVGKIIESCEQKGFKLIAMKFMWAEKESLEKHYVCSTLLPRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAX2</accession>
    <name>T1GAX2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02119046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02119047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02119048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAX2"/>
    <dbReference type="STRING" id="36166.T1GAX2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000388-RA">
        <property type="protein sequence ID" value="MESCA000388-PA"/>
        <property type="gene ID" value="MESCA000388"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1014014_0_0_1"/>
    <dbReference type="OMA" id="CNDATAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052231">
        <property type="entry name" value="Rho_GEF_signaling-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845:SF3">
        <property type="entry name" value="PURATROPHIN-1-LIKE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000388-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="275" mass="31187" checksum="B9D9A37D5C7D46C2" modified="2013-10-16" version="1">SSSSSTTKCTVLQPSKPEFTILVNIRKPEDDTPSLLNLLSNSLKLISNNMRVDDILIYHTFEETSVVISNSCNQNNNLFPNVTVREITTPQALLTYIALKNLPQEFGGTWIHNQEKWIEFMRLAEVIQNQCLATGQRLVTTMSDIRLSDLQGLPTRRQLYAQHRALSRTLMDSDLHNLRKNGPNSVARLHELYKSITSTTSMTTATTTCSSTCNDATAMSTAENDVSSRLNTIVILFNEVDQAAKRLEQLTEQRRERLRELTRQRNLEDEINEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB14</accession>
    <name>T1GB14_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02187516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB14"/>
    <dbReference type="STRING" id="36166.T1GB14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000434-RA">
        <property type="protein sequence ID" value="MESCA000434-PA"/>
        <property type="gene ID" value="MESCA000434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1469850_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="184" mass="20641" checksum="41A2084F4E067644" modified="2013-10-16" version="1">MSKRHIERPFQTFKKESSEAIISHHAGPSDDFNGSLFEETSLGGTGDNTSDSNSNQQLQQLQQQQQQTTPMKEEWYYDDVDVNDIDSLEEPKSPADDEYDYDPRYGNKKRRKRRPGKKALAQMQGVGESSRKSSSRNSSGGVGRGGRRTPREPSLASLRMDPPSPEPQNEPVSFETALGDKLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBE9</accession>
    <name>T1GBE9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000581-RA">
        <property type="protein sequence ID" value="MESCA000581-PA"/>
        <property type="gene ID" value="MESCA000581"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725079_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="7857" checksum="AA0D99C3B97A0EE9" modified="2013-10-16" version="1">MVHCTRVAVGAIEGSVKKQNGIDISTLMMMDELPGVKSIWGSRSHNVSISFIRYVMTSAHKVLAIKSSYGEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBQ7</accession>
    <name>T1GBQ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-(2E)-butenoyl-L-lysyl-[protein] + H2O = (2E)-2-butenoate + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:69172"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:35899"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:69173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:58197"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[protein] + H2O = L-lysyl-[protein] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:58109"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02128615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBQ7"/>
    <dbReference type="STRING" id="36166.T1GBQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000694-RA">
        <property type="protein sequence ID" value="MESCA000694-PA"/>
        <property type="gene ID" value="MESCA000694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_5_0_1"/>
    <dbReference type="OMA" id="HICERYR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF14">
        <property type="entry name" value="HISTONE DEACETYLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="28"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="214" mass="23876" checksum="1ADFC4ACB2C2F2E8" modified="2013-10-16" version="1">MSEFKKKVVKYVYSDSLLYFIDKNPAITGRSKLVHELIKSYGLLGKLPVIPPRICSSAELAQFHSSDYLSALKSGHELEEFGLGYDCPIWDGIFDYCKIVGGSTLAASFDLCRKQSDIVINWFGGWHHSQKDKAGGYCYVNDIVLGIMLMLKVFKRVLYIDIDVHHGDGVEGAFETSNRVFTLSFHQHEPGFFPGSGNSTSTGFASGKESHLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBT5</accession>
    <name>T1GBT5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02038154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GBT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000723-RA">
        <property type="protein sequence ID" value="MESCA000723-PA"/>
        <property type="gene ID" value="MESCA000723"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001341_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052398">
        <property type="entry name" value="Ubiquitin_hydrolase_53/54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975:SF9">
        <property type="entry name" value="ECHINUS SPLICE FORM 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975">
        <property type="entry name" value="UBIQUITIN SPECIFIC PROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="26"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="665"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="842"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000723-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1121" mass="125526" checksum="81D8D98510F94568" modified="2013-10-16" version="1">MIKFRYKRKEPATPTASPISSPSSGSGTGTLPRASPKKTDDQVKSSTLSRRDIKSHQILQNEQIEERDRCINAVIELFQQLQTSSEPALCPEPLRRALASGPLAGRRFPLGCLGDAAECFELLLHRVHSHLSSEDGDLCETQSCVAHRRFAMRMVHYVSASALTAQNSLATRTHQQMSFGQLLRAAGNMGDIRDCPNACGAKIGICRALMNRPDVVSIGIVWDSERPPAEQVHSVLKAVGTSLKLSDVFHQVSDQRWAQNVQHELVGIVSYYGKHYTTFFFHTKLKVWVYFDDANVKEVGPNWEGVVEKCTRGRYQPLLLLYAVPQPPSGSQMVQFAQEMAQRRAITPSPEKNPIGNTRRAITPTPIRSPTCDYQNLNVIQQKIRQANDETDSYISRKTVENVLNAQYQNLNVIQDKIFEDKKNAEARKHHMNLHRSLSAESGSSPPSDGLSIPEHLNQPRRRDSGNWILLIEMVILMILDMILLYMVAKRNGPTAVPQSPTHRNGHPYDPGYDSFSLSSTDSYPPKMHNPQLAKIPESVVLTGDCEKLCHEADQLLEKSRMVEESHDLETALVLCNAAAGRARAAMDAPYSNPHTMTFARMKHNTCVMRARSLHRKILVEKGVEADLNLPEIKHTREGNKEKSGKKQHKIRRKLLMGGLIRRKNRSMPDLTDGNDEIQNKENIPEPPMVTVDDSSVGLTGKDNMSGYISEGHFEYRESNANPNLERSRLMRKSFHGSGRQLTAAKVAPPPPLRISSALTQEAXXXXXXXXXXXXXXXXXXXXXXXXXXXXYHEANLSNISAMSSNTSISEDSCQTIITTCAQIHSEQKYAVAVDEVDTKFEELPPYPSPPGSANHSRQASEDFPPPPPELEPISHQMNQLHLSELSRRKSLDTLEGTTSILAQLQQRQMMKMRQEPQQSDWLQELQMKQNDIRNNNVKSMASRFETPMKSYSSQEILNKPDVRTTMNGLPINGMIHPQNIMTMQTAQLSHPKSIDLDEVDCMMKSKLAQNQIAEEIKEVEMLNRKVSFEPGTKGENECLPSPQPSTTSSTPSMNGNLVLDNNSNSANITAIPTRVYNNAIVKASAKAVECNLCRKKHVIAPAVYCTNCEYYLQMLNSRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCD0</accession>
    <name>T1GCD0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02023481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCD0"/>
    <dbReference type="STRING" id="36166.T1GCD0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000940-RA">
        <property type="protein sequence ID" value="MESCA000940-PA"/>
        <property type="gene ID" value="MESCA000940"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1108160_0_0_1"/>
    <dbReference type="OMA" id="RVIHANM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF415">
        <property type="entry name" value="FI20190P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="73"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000940-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="251" mass="28512" checksum="CB28740621FFE808" modified="2013-10-16" version="1">MSYEDDEDIPYIDGVKGYSPLKKRRTSSMSSNDKSSVGDLSVREEDKENLADGQVSPRIWKKYFTAGGSGLKLFMLFTVLILSQVVCSGSDYFSNYWTQQEYLRGLDEPTELTTYQCLYIYGILILCVVFMTVLRGYMFFSICMRASKVLHNRMFKSILNATMRFFDTNPSGRILNRFSKDMATLSGLSTIRARGLQDLVIKEFDNLQDVHSGAWHLTKSLNYAFGLWLDIVSCAFLTAVTFSFLVGDSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCJ2</accession>
    <name>T1GCJ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GCJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001007-RA">
        <property type="protein sequence ID" value="MESCA001007-PA"/>
        <property type="gene ID" value="MESCA001007"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_7_0_1"/>
    <dbReference type="OMA" id="WDSELAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034763">
        <property type="entry name" value="P14a_insect"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038921">
        <property type="entry name" value="P14a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577219" description="SCP domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="6">
        <location>
            <begin position="57"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001007-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="260" mass="29557" checksum="40ACC615B71D6FFE" modified="2013-10-16" version="1">MKLLGIVFSALLAYCSAQIQQYCRPDLCQRQPHIACISSGGLGKSCPRDTSVVNLAPYKSIILNDINRHRNNIAGGQVQKHRKAARMGTVTWDDELAYVASLNAMTCKFDYDQCHNTARFKFSGQSLGKTMWVDTTLSIQQLLKKQVEKWYQEHVHSNMSSINRVVPSERKQSGRFRLMAHEQSVKIGCSAVRYKEWANNRYYDTFTMTCNWAHNLILGSRVYQSGPIASLCKTGTNPYFPNLCSIAEXNLPQTLYILYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCT5</accession>
    <name>T1GCT5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02074504">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001108-RA">
        <property type="protein sequence ID" value="MESCA001108-PA"/>
        <property type="gene ID" value="MESCA001108"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631248_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001108-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="19689" checksum="F8B771CE649FABEB" modified="2013-10-16" version="1">SFSSEDLKLNDLKKDCFICQESFSNKRDLENHCRRHRNLEIYHDNNHTIDLGNFKVFQTISKFSRDFLFILSSIVENVLLKRIIFAINKFHYPLENIKLEKSKEIMFTMNTKLFEPGHVYPLMISYTRENDNKIYFIQYLFEFKPNKVNYSSVQQRKRTKTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD15</accession>
    <name>T1GD15_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 1">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NMT family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02043021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD15"/>
    <dbReference type="STRING" id="36166.T1GD15"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001194-RA">
        <property type="protein sequence ID" value="MESCA001194-PA"/>
        <property type="gene ID" value="MESCA001194"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126154_0_0_1"/>
    <dbReference type="OMA" id="ERACNNL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00976">
        <property type="entry name" value="NMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="3">
        <location>
            <begin position="67"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001194-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="160" mass="18747" checksum="C806EAD932E5C08D" modified="2013-10-16" version="1">MTLQRTIKLFKLPDEPKTKGFRKIQSKDMNAAHKLLDDYLKKFKLCPVFTKEEFRHWFTPREGIIDLPSSVMQHPVHKTVRAAYSFYNVSTKTPWLELMNDALISARNIQMDVFNALDLMDNKKFLVPLKFGTGDGDLQYYLYNWKCPPMNPEDVALILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEC7</accession>
    <name>T1GEC7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001688-RA">
        <property type="protein sequence ID" value="MESCA001688-PA"/>
        <property type="gene ID" value="MESCA001688"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987154_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001688-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="13361" checksum="BCA5FD75B7A18215" modified="2013-10-16" version="1">SGNNCNASNTNSTSLATSASSINSKSNSNIPGLTAVNNLDKIKSPLNIQQSKQQSGKSNPPFPNTSPNGLNMNLFDAGGGGEIRNILLRTNFFSPPPTRSVTYIFYTLEILTVICSFSSGIGFHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEG5</accession>
    <name>T1GEG5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Seipin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02155876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEG5"/>
    <dbReference type="STRING" id="36166.T1GEG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001727-RA">
        <property type="protein sequence ID" value="MESCA001727-PA"/>
        <property type="gene ID" value="MESCA001727"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049458_1_0_1"/>
    <dbReference type="OMA" id="PAITHMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212">
        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212:SF0">
        <property type="entry name" value="SEIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001727-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17765" checksum="C197065EA68A9195" modified="2013-10-16" version="1">MIYMPAITHMRPVNMQFKACMETGGQCSFPQAHVTLNEKQRLLMQGQEYKVGIKIDMPESPNNQDLGMFMVCSELKDADNLVRAHTCRSAMLEYKSPSIRTIQKLMTLPMILMGFLEENQSIAVEVFPKYVEDVNHPITDVYVEIQSHKIEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEJ7</accession>
    <name>T1GEJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DHHA2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPase class C family. Prune subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02015115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEJ7"/>
    <dbReference type="STRING" id="36166.T1GEJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001762-RA">
        <property type="protein sequence ID" value="MESCA001762-PA"/>
        <property type="gene ID" value="MESCA001762"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586058_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF39">
        <property type="entry name" value="EG:152A3.5 PROTEIN (FBGN0003116_PN PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="3">
        <location>
            <begin position="166"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001762-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="467" mass="52496" checksum="D95F2C27EED29095" modified="2013-10-16" version="1">MIKAIVPVLNVSRKNFPIKTEVKHILGEHDITENQLIFKDDLSENQLNKNEIILVDHHVSPYNSNVVGVFDHRPYNPDCNLNENCKKIIEKVGSCSTLIAGQFLEKTEKPKDYEAPLSLLYHAIVMDTVNFSEKIKIFTPKDLEAATRIEELFPEKFKESSRNELFDKIVRTKADISSLTPYQILNKDAKFLSFNGINISLPGFPMAVQDFVALPNVSNALEGFSEEFSSNAIVLIGQKYIDNGVLRDIGIISRDPKLKEELSESLKKAKNDKSQQLQLEEIKGIPPPSIITTPPPSPKPVLCSDNYENRRVINLSMGNEMSTVNEEVNEYIDDDVGAGYGSHHSRHHHHHHHHHNNNGDNMTVTGIAGNPKDINIVPNTNEYICHNNKLVCSERPEDEQDDEVVLRNPPCNSNNKDKDDDRNILEETDVAKYLIFKKEGEDGADIKGGYFDALLVHASQVSKGSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFB0</accession>
    <name>T1GFB0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02065933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFB0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002047-RA">
        <property type="protein sequence ID" value="MESCA002047-PA"/>
        <property type="gene ID" value="MESCA002047"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673915_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002047-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8770" checksum="AF3529162B0C5540" modified="2013-10-16" version="1">MAFMTPNGKKEIFPTKNNQLDWKYHPKAVAENNEDVPSPTRATTERQRKKNVSEGHQYQNGINFNQKNFKTFKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFB3</accession>
    <name>T1GFB3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">p53 DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the p53 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02126288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFB3"/>
    <dbReference type="STRING" id="36166.T1GFB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002050-RA">
        <property type="protein sequence ID" value="MESCA002050-PA"/>
        <property type="gene ID" value="MESCA002050"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1316743_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011615">
        <property type="entry name" value="p53_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002117">
        <property type="entry name" value="p53_tumour_suppressor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447">
        <property type="entry name" value="CELLULAR TUMOR ANTIGEN P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447:SF16">
        <property type="entry name" value="P53 PROTEIN LONG FORM VARIANT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00870">
        <property type="entry name" value="P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="p53 DNA-binding" evidence="13">
        <location>
            <begin position="10"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602117-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002050-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="23932" checksum="3B29BA5154525EA8" modified="2013-10-16" version="1">MYPLHCISSNQKKYNVIHSNYPQVIYEGSKEGRTADERLSIVFPLPCSNNKRVGENIVETIPIMFVCQTSCIGRDDIAVICTLENSSEVLARTTMQVKICSCPKRDCQKKDEESGVPSRSNRKRPMQTQNPMSEGKRIKVENTIQNDERSVLSFHFDAPNSYIGLKYCLHTRVFVKLELESDPENDYLKTKLSELNNKIIKLERELGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFB8</accession>
    <name>T1GFB8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFB8"/>
    <dbReference type="STRING" id="36166.T1GFB8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002055-RA">
        <property type="protein sequence ID" value="MESCA002055-PA"/>
        <property type="gene ID" value="MESCA002055"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161734_0_0_1"/>
    <dbReference type="OMA" id="RYPRFGA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05793">
        <property type="entry name" value="TFIIF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002055-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15968" checksum="D2B8347125491658" modified="2013-10-16" version="1">MNNREGKSDAVGGGSSKKPSHVQEFSIRVPKTQRKKHHVMRFNATLNVDFAQWKVVKLERENNMKEFKSLEEDQPKFGAGSEYNRDLREEARKKKFGIISKKYRADQQPWILKVGGKPGKKFKGIREGALAEFLNFSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFC0</accession>
    <name>T1GFC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GFC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002057-RA">
        <property type="protein sequence ID" value="MESCA002057-PA"/>
        <property type="gene ID" value="MESCA002057"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_861348_0_0_1"/>
    <dbReference type="OMA" id="FTERCAF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="4">
        <location>
            <begin position="48"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="2">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="173"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="260"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002057-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="323" mass="37164" checksum="B5696F8238A58263" modified="2013-10-16" version="1">MRNLRFAHKHQNHSRRTVVEKLQAYAFPLSNGGKLFAFSYSETFPENGWNVYEPLAELRRLGLPNDSWRITKTNENYTVCDSYPAVWAVPKVASDDFLKKVSQFRSRNRLPVLSWIHPKTLATITRCSQPLVGVSGKRSSDDEAYLHYIIDGNARSEKLSIMDARPSANAIANKAKGGGYESEESYQSVEINFLDIHNIHVMRESLRKLKEVCYPTIDDSKWLSSIDNTLWLKHIKFILAGAIRIVDKIEIMSTSVLVHCSDGWDRTAQLTSLAMLLLDPYYRTLKGFEILIEKEWLSFGHKFQQRIGXKYIKISHFVIFFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFQ1</accession>
    <name>T1GFQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02050860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFQ1"/>
    <dbReference type="STRING" id="36166.T1GFQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002198-RA">
        <property type="protein sequence ID" value="MESCA002198-PA"/>
        <property type="gene ID" value="MESCA002198"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004383_1_2_1"/>
    <dbReference type="OMA" id="INYDSHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070877">
        <property type="term" value="C:microprocessor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058938">
        <property type="entry name" value="Helical_CED_Drosha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26050">
        <property type="entry name" value="Helical_CED_Drosha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="3">
        <location>
            <begin position="570"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002198-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="689" mass="80110" checksum="03A182263ABBA972" modified="2013-10-16" version="1">MSGNQNNNPIPYFNPNVPPPSIPPRFYFHRFVPPPPPAVVTPVPATVVSSAPTTTRKRPSRFSDAVPKPVISVPPPPVRFDELKKIDRRKRSENETEREKILRKWRSNYCETSEQMSKKLAELADSEEKQYWIRSSPSDIYYKRTVQQNGIEVEATPRLEALCELFERELIIRAENAREAMPKYTPPPQVCSSSDDSSDEEFHPEQDCSMEELSMKTQHPHRLHADLWHNEPGEMNDGPLCRCSAKSRRIGIRHGIFPGETGYNKCNPDTNNANRLFHYRITISPPTNFLTKTPTIIKYDEHEFIFEGFSLFSHTKIDELHVCKVIRFNIEYTIVYVEEQMPENFTIRELDLFSKFLFTELLELVDFAQKPNTALDSCNCFHFMPRFVRDLEDNGKEILSMSEVLKFLLIILDHFQYQWQNYADVLKGMVVTKPGHRPCSVRIDQLDRNVSDIPECIKEGVTHPAIVHFGIRPPQLSYAGNPDYQKAWREYVKLRHLMANMPKVSYEEKRKLEEKEQKLQDMRSLGRMKRNVTVAISSEGFYRTGIMCDIVQHAMLIPVLAGHLRFHESLSFLETNLGYKFKNRYLLQLALTHPSYKENFGTNPDHARNSLTNCGIRQPEYGDRKIHYQNTRKRGINTLINIMSRFGKEYETLSNITHNERLEFLGDAVVEFLSSIHLFFMFPDLEKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS1</accession>
    <name>T1GGS1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02020073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02020075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002600-RA">
        <property type="protein sequence ID" value="MESCA002600-PA"/>
        <property type="gene ID" value="MESCA002600"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2253109_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="12131" checksum="3C65DF99C1A5D86A" modified="2013-10-16" version="1">MLYNKNRDAVFYENNMSRYPIVGDFSNEVIISLSSSYTNTNEVDGVDETERTPTEEDTIENDNFDEDFEEHSDIKRSSRTTKGIPPDRYEATCVTTLDLYERRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH51</accession>
    <name>T1GH51_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH51"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002736-RA">
        <property type="protein sequence ID" value="MESCA002736-PA"/>
        <property type="gene ID" value="MESCA002736"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657303_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002736-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8838" checksum="D2DEC13B3125260B" modified="2013-10-16" version="1">MEKRRAKSFDRNKLKGKDKARNVESLETKTALKDIKKLPITSGDIGIYSFPFHPFHDHCSPFRYLSERNDDQSLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI27</accession>
    <name>T1GI27_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02135960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003094-RA">
        <property type="protein sequence ID" value="MESCA003094-PA"/>
        <property type="gene ID" value="MESCA003094"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796890_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003094-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7368" checksum="383D2B2A2881D3D3" modified="2013-10-16" version="1">MGNGTIKRAFNRGTLQGGVISPLLLLTISRGVVKFKPYGHGGGKYHGQKCSSPTKHGPFQGKKLFQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI46</accession>
    <name>T1GI46_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151946">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="36166.T1GI46"/>
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        <property type="gene ID" value="MESCA003113"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_701279_0_0_1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003113-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="43252" checksum="8CD0F10C6501EFA5" modified="2013-10-16" version="1">ESESNVESESIVAVTTEKPLELVAVTEEVIVPVKVLNPGDREYLTPNLVGLQNMAMSNLPVTINFVNVIEAYREIVNGVRYELLINAVNTTETSDDKDVVCRLVTEILKLSDTEADLASQDSINQIYKTNTIFDGSAIGDDEDGLAKIESQIIQPKDKVVAQRESTTTTTTTLAPIVVADEENTDEDVETTTLEELSDDSKKWLDGFFGIFDSPTKQSETKASAENVLANPESSTVNNEIIDQNEESGRAKREVLVGGKSDFSKKEAEEKLSKTFSLISKGDGSPDIKIKKIYSAESQVVAGSLFTITGDFIINNEEKDNCEVKLWERPWLGDEGGQTEFNCPDGHKQKYKHKVAKRSIGVKGAPKDFPKDEALELANKVLKSIATGEGXNQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIJ1</accession>
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        <submittedName>
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    </protein>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="36166.T1GIJ1"/>
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        <property type="protein sequence ID" value="MESCA003270-PA"/>
        <property type="gene ID" value="MESCA003270"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_850696_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
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        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
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            <begin position="219"/>
            <end position="277"/>
        </location>
    </feature>
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        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003270-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="327" mass="36672" checksum="787682EC50AA8042" modified="2013-10-16" version="1">MLRSAAEYIESQQSHQNLEDITSPHNIYTHHTTMSLPGSSRIIKNHLGNGEPIQNYHHPLDPHDDMEIMKSSHSHLKQHSNDVIVTTITEDMQSPNDVEIMHQQYHSHLHQQQIHNQRNIRHHETISVIVQPNEDCSRDSHGGLMSPQKLTPSSVTSLQSGGGTNDDLDSTTYQTLTSVVNNYNRSSPTNDFSPNSSAYATLTPLQPLPPISTMSDKFAYGHVNNSSNNENNENPNTGYHNYNNSNGSNMQKSPAFSQDHHHSPQKLSVNVNSSSPNPYNYNNEIVIGTTDQFNNGRHNTRQSQQLSPNLNSYATINRVSDLSHMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP9</accession>
    <name>T1GJP9_MEGSC</name>
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        <submittedName>
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        </submittedName>
    </protein>
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            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177476">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003699-RA">
        <property type="protein sequence ID" value="MESCA003699-PA"/>
        <property type="gene ID" value="MESCA003699"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3038391_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577428" evidence="1">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003699-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="55" mass="5864" checksum="D3457F40E0F173E7" modified="2013-10-16" version="1">MSKISGVIAILFMAALAAAASAVAAAASPVFNINLEYFNFLPHDDQNDPYVLYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK34</accession>
    <name>T1GK34_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
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        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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                <person name="Hughes D."/>
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        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        </citation>
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    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA003848"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375149_0_0_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577437" evidence="2">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003848-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10284" checksum="845173DABA1302A2" modified="2013-10-16" version="1">MTLSKTLFMAIFVVFAFEGVLCQQESSNESDVVESARTFGHHFLKRISFAIIPGAFVVGAVTTLLAALTVVSMNGLGVGLVFPVRIGKFDFQLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKH6</accession>
    <name>T1GKH6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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        <name type="synonym">Phora scalaris</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
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        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA003999-RA">
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        <property type="gene ID" value="MESCA003999"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1519587_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="30"/>
            <end position="125"/>
        </location>
    </feature>
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        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
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        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003999-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="19990" checksum="C825012EB39B91F5" modified="2013-10-16" version="1">MLKKGIKGQNQLEVAIGDGMEFTIHGDMKNNKSKKKLSNNASIIENSINHQDNRLEHELNHRSMSFQDEDTASIKSYGSHKNRPFKDESHKGSAETFGDCDEEKRDVSKEDLGIEEDVDEEGEECPLDGEMIIHAGQDEEVLDEFPADCCPDSYYVKFPFLAGDDDLHFGKDGEIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLR3</accession>
    <name>T1GLR3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02065562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02065563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004476-RA">
        <property type="protein sequence ID" value="MESCA004476-PA"/>
        <property type="gene ID" value="MESCA004476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2375152_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10836" checksum="12D95F5ABB8ABA99" modified="2013-10-16" version="1">MTEVCKVRNDTLIVEFRTCANSLSRIPFRTLESVVVDSKIKYILEIHESVGSLTVVDCGRIVFSFEPYKSAGPEGVFPADIIQYKNVQVLYEYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM21</accession>
    <name>T1GM21_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GM21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004589-RA">
        <property type="protein sequence ID" value="MESCA004589-PA"/>
        <property type="gene ID" value="MESCA004589"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031679_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004589-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="29207" checksum="AF51DF778E8DBAD9" modified="2013-10-16" version="1">MLLQLLKLQSHLQLVIIVMENMFANIFNQAAANPAPQGPVTPNIPNPQDSTSTSTPATNQVPINPPFPFGMGAAVPPPFPFVAPFTIPPPPMPPNLSTLSDEELRALEGTERQNVEXXXXXXXXXXXXXXXXXXXXXXXXXXXAPTFVSGNQQPQQQQQSPLSTTSVDYFSTTDSPRPSESDSKKQPTTAAPGVVNSEASTSKLDLSKEVSIEDIGSEEDRPKSQGDQQPTSTLITPATAENDSSAPSADLNEVRRRWNQKFAQQNNTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMJ6</accession>
    <name>T1GMJ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02060805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMJ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004771-RA">
        <property type="protein sequence ID" value="MESCA004771-PA"/>
        <property type="gene ID" value="MESCA004771"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412095_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012542567" evidence="1">
        <location>
            <begin position="16"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004771-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="23027" checksum="788D2BF243D430E9" modified="2013-10-16" version="1">MRTIFISIIFQSVWTLPLSSNNERYIHDAVRTMREDLKFYEELENQTLLFNASPTMSGISNLLSIYLVKSRLNEIQKREIKRIHQQQLIKEELEIVAHHFDYINEHNFEKSDFESIRGKLLEDIKDNEEILDLVNDIHQSVSEQIDIKKERIEGKMDMFENIFLTHSQFRRITNMERPPNVLTVKRTRQIAPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMS0</accession>
    <name>T1GMS0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02091900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMS0"/>
    <dbReference type="STRING" id="36166.T1GMS0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004850-RA">
        <property type="protein sequence ID" value="MESCA004850-PA"/>
        <property type="gene ID" value="MESCA004850"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827518_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001307">
        <property type="entry name" value="Stretchin-Mlck, isoform V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF171">
        <property type="entry name" value="STRETCHIN-MLCK, ISOFORM U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="53"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004850-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="15694" checksum="8744DDAF35350718" modified="2013-10-16" version="1">MIADGLGVYMIEIKPSESCDEGEWKCVVTNEEGSVGISTSVVKMEIPRNYRRPRFMESLKAVLTDEGLVSFECKVVGFPTPILRWFKDGQELKPGDVYQLTGTNSLGTYCCIAQNCMGENSSVAVLTLEDIQNQLTDEERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMW5</accession>
    <name>T1GMW5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome c oxidase subunit 5B, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02008305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMW5"/>
    <dbReference type="STRING" id="36166.T1GMW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004899-RA">
        <property type="protein sequence ID" value="MESCA004899-PA"/>
        <property type="gene ID" value="MESCA004899"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127178_1_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004899-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="8191" checksum="48461A12A3D99BE2" modified="2013-10-16" version="1">MSDPLEHATGIEKRELLAKQAGNDNPFDMKVFKRGEEDQTYVQWMWLQKDQPRRCECGHWFQLVEKAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMX2</accession>
    <name>T1GMX2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMX2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004906-RA">
        <property type="protein sequence ID" value="MESCA004906-PA"/>
        <property type="gene ID" value="MESCA004906"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087572_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004906-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13588" checksum="0FBABD238796A9D9" modified="2013-10-16" version="1">MADKISRDRGFHYYCEKHRNISVSELLKKLAKLQSFHVELKLLTDKYKDILEAPVVSDLRKLEKEKLTEISTDSPSTNMGRGLRSSSKRQVDSSDEIIKKKKTAKEYVYISEKQPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNC1</accession>
    <name>T1GNC1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit beta type-2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02152837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNC1"/>
    <dbReference type="STRING" id="36166.T1GNC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005068-RA">
        <property type="protein sequence ID" value="MESCA005068-PA"/>
        <property type="gene ID" value="MESCA005068"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_12_1_1"/>
    <dbReference type="OMA" id="FCINELY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005068-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16285" checksum="DCF53D8E1BA50F42" modified="2013-10-16" version="1">MRNGYELSPKAAAHFTRRNLADYLRSRTPYQVNMFLGGFDDKEGPHLHFIDYLANGKSLPFGAQGYGGFFSASILDRYYHNKLTEAEAYDIMKKCVAEIQKRLIINLKNFKVVVVDKNGIRDLEDITSVKLEAAIEAAQGVHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNJ8</accession>
    <name>T1GNJ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005152-RA">
        <property type="protein sequence ID" value="MESCA005152-PA"/>
        <property type="gene ID" value="MESCA005152"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191714_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005152-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7191" checksum="8FD049327157162E" modified="2013-10-16" version="1">MTPQEEMLIFNSNEDVPPPTTEEVRSGIQRLKNHKSAGSDGISAELLKAADINIIDAFRQLLVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNL7</accession>
    <name>T1GNL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02165157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNL7"/>
    <dbReference type="STRING" id="36166.T1GNL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005174-RA">
        <property type="protein sequence ID" value="MESCA005174-PA"/>
        <property type="gene ID" value="MESCA005174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048451_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="252"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="288" mass="32630" checksum="5A91394AE6B32A37" modified="2013-10-16" version="1">MEITRGFYDLCIPFSDRANLKKLVKELIERIWIQDGSNRANLRSHEKYNREEAGHFSCSGRSQFFEGIRITNQNFQPTYNIYSDVSVSHPMSKSSNIQKFHIIAGRPKSDAAWTHCCSTFVGDIISFSSEDPKILINRKTYLLGVNRGMFFEIRYSEAIRNSSHRKEMIKVSHNFHSRGKSKNVIMSSGAENMFELRGPYDVANLGLIFGLTEEKGKNAVGANCRKILLNAESRRAGKTFFIMTAKGPIVFSSSEDEGSDEDGSALEDDAMDEIENDLQQPFKKQKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNV0</accession>
    <name>T1GNV0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02181405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNV0"/>
    <dbReference type="STRING" id="36166.T1GNV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005261-RA">
        <property type="protein sequence ID" value="MESCA005261-PA"/>
        <property type="gene ID" value="MESCA005261"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888150_0_0_1"/>
    <dbReference type="OMA" id="TWHSFPE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00034">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000031">
        <property type="entry name" value="Heterochromatin protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008251">
        <property type="entry name" value="Chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01393">
        <property type="entry name" value="Chromo_shadow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00300">
        <property type="entry name" value="ChSh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Chromo" evidence="5">
        <location>
            <begin position="68"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="14369" checksum="00DB0759F51BCA78" modified="2013-10-16" version="1">MHLTKNSLTKIPIAGSTDLNNSSIPTEGKDGGVKKRAASSGPGVDGKKKKLAEADTTDDKPCGFDRGLEPVKIIGATDASGELMFLMKWKGCDEADLVPAKQANVKCPQVVIQFYEDRLTWHVPGTDNAEQNAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPK4</accession>
    <name>T1GPK4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149014">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149015">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPK4"/>
    <dbReference type="STRING" id="36166.T1GPK4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005537-RA">
        <property type="protein sequence ID" value="MESCA005537-PA"/>
        <property type="gene ID" value="MESCA005537"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027023_0_0_1"/>
    <dbReference type="OMA" id="ISHYTSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="367"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005537-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="52305" checksum="511BB416DB48BBE8" modified="2013-10-16" version="1">MIDYIDAKNHEGREKAQIPHLPWSCERSLPKYRPSKPISHYTSEEVVRLSRKAAAQAQQDLINARVAIRTPFSVKKTADAARVTKHIQPDTVVERENLRYTQRLEEIRFQERRDYLRMLHHIQRMDENQVAISAELKRAIRGKSAHAITAALLAETDRNLKNARAEEEAFIAAQSAARASSVTRKQTAIEIEQLNARVVEAETRLKTEVMRIKKKLQIQITELEMSLDVANKQNIDLQKIIKKQSLQLTELQAAYEDVQRQLQQVVDQNTVAQRRLQTLSGELEEARVNLDSATRAKRAVEIQYEDAQTRISELSTININLQSSKVKIEQELAVVASDYEEITKELRISDERYQKVQVELKHTVEILHEEQERIVKIETIKKSLETEVKEGVSQQSVTRVRRFQRELEAAEDRAENAETNLNMIRAKHRTFVTTSTVPGTQVYMVESTSRTITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPS7</accession>
    <name>T1GPS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PHLPP-like RA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPS7"/>
    <dbReference type="STRING" id="36166.T1GPS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005619-RA">
        <property type="protein sequence ID" value="MESCA005619-PA"/>
        <property type="gene ID" value="MESCA005619"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066673_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHLPP-like RA" evidence="2">
        <location>
            <begin position="38"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005619-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="261" mass="30541" checksum="9A5E4AA74514A7DC" modified="2013-10-16" version="1">MGVIDQNDFLSQLQYNKNINQIYGSKSSFHEEKDIGWIRVFCGPDRSDLLTIEPSRMVRCSVHTTTKDIVKCLNLPKEYTLWVQFGCSEFKRFQNHEYPYKLQNEFYINFGYTHISRRMRLEIDPEMKYLLRFFIGPSDISECPGVLKSGKVDILKGLVYPQWQYRSLAIIGSVLYICPLNSYMAPEVFDISEAEVLEHSPKYDRLILKLVTKFPYEKNNNNSYKSKITTKVQTILWTIIQIKLCFWALKNHGREICGTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPW7</accession>
    <name>T1GPW7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gamma-glutamyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02017085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPW7"/>
    <dbReference type="STRING" id="36166.T1GPW7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005662-RA">
        <property type="protein sequence ID" value="MESCA005662-PA"/>
        <property type="gene ID" value="MESCA005662"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_4_2_1"/>
    <dbReference type="OMA" id="PVCDHRR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF72">
        <property type="entry name" value="GAMMA-GLUTAMYL TRANSPEPTIDASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="1" id="KW-1202">Platelet aggregation activating toxin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0800">Toxin</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="355"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00084097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="51819" checksum="2A6B4200A9D8E7D9" modified="2013-10-16" version="1">MIEEGGSAADSIISVLLCEGVVAPHVVGLGGGFMATIYNKEKGTMESLVARETAPAEAHMYMFLNASSVDGALTVGVPGELKGYWELHKKYGKLPWKQLFSPAIQLCKEGIRVTPYMAYAMKTIDESYKYEPSLKDMLIDPKTNDYKIMGDLIFRPVLAKTLEIIADKGADVFYKGEVGRNLVKDLKERGGIITEEDLANYKVKWSDPTKAEFINQYSVYSTPMPGGGAVLTMILNLINEAKNTYKQMTDPKFADLIRPLIRDDKVFDDLKYYGSKSGGPEDHGTSAMSVLHPNGDAISVTSTINFYFGSKIRSPSTGIILNDNLDDFSTPGIVNTDGDLSSPYNFIEPGKRPLSSMCPSIVIDKKGDVKMVLGGAGGTQIVTAISQVLLKSLVFQESLDETINSKRLHHQLLPTLINYEEGFDPEVLEYLVGKGHAIESFGHRSTSAVTAILVGNLTTEVAFDKRRGGSAAIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQA0</accession>
    <name>T1GQA0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="T1GQA0"/>
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        <property type="gene ID" value="MESCA005804"/>
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        <property type="term" value="F:protein tyrosine phosphatase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF540">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 99A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005804-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8890" checksum="45DE1EE7BFBE9ED2" modified="2013-10-16" version="1">MITNLVERGRRKCDMYWPKEGTEVYGVIQVKLISEEVMATYTVRTFHIKHLKVEIRCICLVAKGAMENVGFAKNKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD7</accession>
    <name>T1GQD7_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Megaselia scalaris</name>
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        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
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            <taxon>Megaselia</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of sodium ion transport"/>
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        <property type="entry name" value="TIPE PROTEIN TEMPERATURE-INDUCED PARALYTIC E"/>
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    </dbReference>
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        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005841-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="20965" checksum="3DAC25C7F553A43E" modified="2013-10-16" version="1">MAGAKDCLNGTIIPQNSSLIPYPTMNFSVFWNIYEKSNIELDPTQRFLPKQSTLTIYNSSKLYINLEGCVNTLRGECIEFVERHGSDGREHTGQSKYPCFYHKSDPSYALTRHDPDKTYRELLVTIIVPSVLFVVSSISLCLVMTLRCGVFAVKMATVMMASRIWQKTRKIIKMAMMGRKRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
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    <name>T1GQG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
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        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA005871-RA">
        <property type="protein sequence ID" value="MESCA005871-PA"/>
        <property type="gene ID" value="MESCA005871"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838562_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005871-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7281" checksum="99B10E9FCAEEF121" modified="2013-10-16" version="1">VFINEATTPRQLHINIKGGGSIKKLGVQNIANFHGVPEGKQPKMTMNNFVNIKKDQKPPNSDIITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQT2</accession>
    <name>T1GQT2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <authorList>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005997-PA"/>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="17875" checksum="33188D596C1B5567" modified="2013-10-16" version="1">MRHRKQFAQHFQVKFHKINLQKIEKRYNQILERSQHCSLATSILTKALSRYKMHVAKIRALDRWKRWGSRNIPGRIEQLIEDNDENRTNFFEEFSVISSEPAFLFFLFLFLYAPAGISSTPKKECSAIQFLTKSGEAVHKCDLPPWKEGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQV4</accession>
    <name>T1GQV4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
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        <name type="synonym">Phora scalaris</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
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            <authorList>
                <person name="Hughes D."/>
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        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MESCA006019"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3299930_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006019-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="40" mass="4582" checksum="3905BDF471D8C04B" modified="2013-10-16" version="1">MIFEQEQIHTSLHMSHPSQALCADPLSYVWKPKQLISSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR84</accession>
    <name>T1GR84_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR84"/>
    <dbReference type="STRING" id="36166.T1GR84"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006161-RA">
        <property type="protein sequence ID" value="MESCA006161-PA"/>
        <property type="gene ID" value="MESCA006161"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755622_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000002">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="2">
        <location>
            <begin position="204"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006161-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="367" mass="42226" checksum="6C4C944C3E95C813" modified="2013-10-16" version="1">MNLVLSPSNCLQSQGINNLQDVWDVGEGECNVLLESRFEKLYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFVTQYYGLVLDLLVLGLHRASEMAGPPQMPNEFLTFQDTQTETSHPIRLFCRYVDRIHLFFRFSADDAKDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDKNKSKTTYLLYHCQHCRQLHHLPNLRIGGGAGFLYGVSPPDNPQVKEIRCIVMPPQWGTHQTINLPNLLPNHQYLKDMEPLGWIHTQPNELPQLSPQDISTHSKIMMDNPVWDGEKTIVITCSFTPGSCSLTAYKLTPSGFEWGSKNTDKGNNPKGYLPSHYER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRU1</accession>
    <name>T1GRU1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02165946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRU1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006383-RA">
        <property type="protein sequence ID" value="MESCA006383-PA"/>
        <property type="gene ID" value="MESCA006383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1697543_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="155" mass="17956" checksum="A1659AEEA8646605" modified="2013-10-16" version="1">MLDVGELDALMIIYRVTLSKTPYSLETVIDSACSFHTTQHISKRHLAHPQQTVKYPNYHDDILKEEVTIVGLKVVLQPKHFRFKSLLLVCRAQIFNIYENSTEKHVELDGASNQIPFDDDIYPYPYPRPAGSSPFQDLDPDIKMNHIKEINRFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS63</accession>
    <name>T1GS63_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02192518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS63"/>
    <dbReference type="STRING" id="36166.T1GS63"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006511-RA">
        <property type="protein sequence ID" value="MESCA006511-PA"/>
        <property type="gene ID" value="MESCA006511"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002173_9_3_1"/>
    <dbReference type="OMA" id="CNEHELQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000014">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF3">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="35"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006511-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="345" mass="39534" checksum="9F14DE32C0A82D16" modified="2013-10-16" version="1">MGLTESACASPRSALITIHRERIVEDGYRQLAALKAHSLKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQADYSCIDELPSFDNELYKSLTFIKHYKQDVSELNLTFSVDQDVMGKIVTHELHPGGKTRAVTDNNKITYIHLMARFHMYTQIREQTSAFNRGFRSIVNPEWLGLFSAPELQRLISGDTVPLDLKDLRKHTQYYGGFHDSHRVIGWLWDILCRDFSEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVGDDEILEIPLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSH3</accession>
    <name>T1GSH3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02177855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSH3"/>
    <dbReference type="STRING" id="36166.T1GSH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006631-RA">
        <property type="protein sequence ID" value="MESCA006631-PA"/>
        <property type="gene ID" value="MESCA006631"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004826_8_0_1"/>
    <dbReference type="OMA" id="PCANNTK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050969">
        <property type="entry name" value="Dev_Signal_Modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7, 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949:SF32">
        <property type="entry name" value="WNT INHIBITORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006631-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10243" checksum="6D3BD38E47D9B999" modified="2013-10-16" version="1">CQCTKGYFGNRCEFSRCTIPCANNTKCIGNNKCKCPRGLSGNHCEEGVRLQRQACGKRCGKHGTCQDNKCLCHKGFYGKRCNAREKRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT59</accession>
    <name>T1GT59_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT59"/>
    <dbReference type="STRING" id="36166.T1GT59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006880-RA">
        <property type="protein sequence ID" value="MESCA006880-PA"/>
        <property type="gene ID" value="MESCA006880"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075844_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006880-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13915" checksum="BBF1266F2F5ABF42" modified="2013-10-16" version="1">MLDSMKIPIANIDKKSLIDVARTSLRTKLYPKLADILTDYAAQNNHRHFFIAMVMVHVAHHSDMPKRLESCYILTCNVFLEYEKSKVNSWFLKENSWILDARTEKETLRLNRQDLCKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTD5</accession>
    <name>T1GTD5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02097227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTD5"/>
    <dbReference type="STRING" id="36166.T1GTD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006965-RA">
        <property type="protein sequence ID" value="MESCA006965-PA"/>
        <property type="gene ID" value="MESCA006965"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1662798_0_0_1"/>
    <dbReference type="OMA" id="NTKHIIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="9">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006965-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="18474" checksum="49958A907CABD61D" modified="2013-10-16" version="1">MNTKHIIKHGKHFERALATQIIMLAPMAPHFASELWSKFCSTPNRLNPESDELQWNEDVLCQRWPSVDLQYKLDFTVRINGFDNAVIKIPRINLDKLTYEEAMDIAFNTDSVTSYLTDKKIRTTNLVHYPGIEAILNIFVDKVKKPKKDNNENEKVVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTE2</accession>
    <name>T1GTE2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTE2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006972-RA">
        <property type="protein sequence ID" value="MESCA006972-PA"/>
        <property type="gene ID" value="MESCA006972"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_994952_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006972-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="280" mass="31446" checksum="877D386FB8EF8BAF" modified="2013-10-16" version="1">MTFSNVVMNGPGCYGKGGGQDKKASKRDRSQNDIMPHPGKRISSDKDRPSTSGTKSSNVVRGDKTVRNTHSSLSNVKKKTDLTASAPTTSNEIDEKKARMQSKPEVKSIDKLPFSENESEGQYYGRKYLGPQNECGQFLMKSAISKMVKFITDKPKQHFECCNDQFYTDPIIDVASSVYTKCKTKFGEDKSTCFHSCIYMEAGFYGNNGLDTTVMRKMLGPANMVGEDGDWKKSNINKWMDKCFKEIPGGIECSQEIVDIDNCFWHHMFTNCPSYNPEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTF1</accession>
    <name>T1GTF1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTF1"/>
    <dbReference type="STRING" id="36166.T1GTF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006981-RA">
        <property type="protein sequence ID" value="MESCA006981-PA"/>
        <property type="gene ID" value="MESCA006981"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834057_0_0_1"/>
    <dbReference type="OMA" id="HEISKPY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
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        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 7, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006981-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="6948" checksum="7BD7813A0B329008" modified="2013-10-16" version="1">MEKTFRGGISLETEKTESGTTSRLLLTRATAKDSGNYTCAPTSAIPASASVHVLNVQKQIGFNGCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTG3</accession>
    <name>T1GTG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="MESCA006994-PA"/>
        <property type="gene ID" value="MESCA006994"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF3">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactosyl_T"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006994-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12485" checksum="88FC73456A57D43E" modified="2013-10-16" version="1">MDISKSVHQLESHEVPDLDPINFFNFSYIHKPLCETTQKTLTLIVKSATMNFLHRQAIRQSWGNQDIKYRNQTSLRTVFMIGSSESSEINSKIASESNQYSDIFNRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTI3</accession>
    <name>T1GTI3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007015-RA">
        <property type="protein sequence ID" value="MESCA007015-PA"/>
        <property type="gene ID" value="MESCA007015"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3430070_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007015-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="19" mass="2213" checksum="FAD5F7892148110C" modified="2013-10-16" version="1">MIISYHPPIFAPLKRITKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTP7</accession>
    <name>T1GTP7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transferrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02184099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTP7"/>
    <dbReference type="STRING" id="36166.T1GTP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007085-RA">
        <property type="protein sequence ID" value="MESCA007085-PA"/>
        <property type="gene ID" value="MESCA007085"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011309_2_0_1"/>
    <dbReference type="OMA" id="DEWSINS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13529">
        <property type="entry name" value="PBP2_transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000095">
        <property type="entry name" value="Lactotransferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016357">
        <property type="entry name" value="Transferrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001156">
        <property type="entry name" value="Transferrin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018195">
        <property type="entry name" value="Transferrin_Fe_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485">
        <property type="entry name" value="TRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485:SF29">
        <property type="entry name" value="TRANSFERRIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00405">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002549">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00422">
        <property type="entry name" value="TRANSFERRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00094">
        <property type="entry name" value="TR_FER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00206">
        <property type="entry name" value="TRANSFERRIN_LIKE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51408">
        <property type="entry name" value="TRANSFERRIN_LIKE_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Transferrin-like" evidence="8">
        <location>
            <begin position="15"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="8">
        <location>
            <begin position="427"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="542"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="129"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="430"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="534"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="562"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007085-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="784" mass="89913" checksum="727EA936238C8EE9" modified="2013-10-16" version="1">TAQYHNFNEHRAEKISWCTKNLNEYWKCENLTITLERDRALFDDKFLNLTCVLAYSADECIHHIDHDRAHITTLDAGDLFVAGRYHSMIPILQEKITGGFTNYYSVAVLKKGTLPNVHSLRDLRNTRACFPWVGSLAGWILPIYTLLREGGMEITDCNNQVKTASNFFNSSVLCIRYSTNIIQLETIQTSELCSLCTGLIPGGRCTHKDPYFGYDGAFRCLMESGDVAFLRHSTVFEMIQGNEFKNIPADKFELLCKDGRRVPITEYQQCNWGLVPTDALVTSSARTFKERKQFQRFLMKISELYSDPKSRQNFTFFQNQNQNQNSNDFNRNNNYFGNNNNQQYDPFNRFGRFNDFSSTTTTTQRSYYEFPRTNDEERPQNGTFEKFDIFESKRYGRLNSLFNDATKYLDKSMEFIYGIRDCPVGRMTLCVTSEHELEKCIKMRTALKGQLLKPELSCFKAHSQITCMQSIQTGRADVAVFDAGDVYTGGLNYDLIPFMSEIYNLGEPDYYVVAVGKEEDPDTDLTYLKGKYTCHTGINTAAGWTYPMAYLISNGWIRPYGCDSIRAAAEYFTKSCLPGALSNEYNTGVPYDSMCDLCHGTSYHYCRRDASEDYYGHTGAFRCLVEGGGDVAFVKHTTVLENTGGKRKEWWARNNLHDEFELMCPDGTRAEITEYRRCNLGKVKANAIVTRGGNGFNETELNTFQNLFIYAQQLFGRKTKMSLVSDATQQLRVIPREERRYDLYLGKDFMRARRITDCNSGVAQFSAHLALVLSVLLFTMTNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUE7</accession>
    <name>T1GUE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lin-7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUE7"/>
    <dbReference type="STRING" id="36166.T1GUE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007357-RA">
        <property type="protein sequence ID" value="MESCA007357-PA"/>
        <property type="gene ID" value="MESCA007357"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097962_0_0_1"/>
    <dbReference type="OMA" id="TDMATMT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06796">
        <property type="entry name" value="PDZ_Lin-7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000076">
        <property type="entry name" value="Protein lin-7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017365">
        <property type="entry name" value="LIN7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051109">
        <property type="entry name" value="MAM_complex_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14063">
        <property type="entry name" value="PROTEIN LIN-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038039">
        <property type="entry name" value="Lin-7_homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="66"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007357-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="18651" checksum="5E290611A081D241" modified="2013-10-16" version="1">ALNLQRDVKRSIELLDKLQANGDIPTTKLAALQKVLQRSQDLRASATAKATVAAFAASEGHAHPRVVELPKTDEGLGFNVMGGKEQNSPIYISRIIPGGVADRHGGLKRGDQLLSVNGISVEEENHERAVDLLKQAIGSVKLVVRYTPKVLEEMELRFDKQRTLRRRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUI4</accession>
    <name>T1GUI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUI4"/>
    <dbReference type="STRING" id="36166.T1GUI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007398-RA">
        <property type="protein sequence ID" value="MESCA007398-PA"/>
        <property type="gene ID" value="MESCA007398"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847397_0_0_1"/>
    <dbReference type="OMA" id="MELWDFS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF63">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577638" description="Amino acid transporter" evidence="8">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007398-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15390" checksum="5F178540141F6EC7" modified="2013-10-16" version="1">MLKCLIVPLLVSSITSAIGSLDLSMSGKIATRAILYYFITTISAVILGIMLVTTIQPGKVGRIDEFITEDVERNSKVLTPDTLMDLIRNMFTDNIIQATMFQYRTEILQPKNKSMAEASMELWDFSGKHKDGSNVLGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUN4</accession>
    <name>T1GUN4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUN4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007451-RA">
        <property type="protein sequence ID" value="MESCA007451-PA"/>
        <property type="gene ID" value="MESCA007451"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1628941_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007451-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18732" checksum="DBDA427D91F50011" modified="2013-10-16" version="1">MHKCIEVCKRFKDNKAPRPDGIPNRLLKLAVEKGISKNVTIKKYFPVPGKHNISFCYQKGKVNTENMQPICLCVMYFTFADDPSAESSYQQSSRFLAVDFQDVRVSNGGFGPMIPNDVGSDLIAYTWSYLRSPSSSSFSVFEKLKLYLMLFYRQRLCRLRSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUU2</accession>
    <name>T1GUU2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rad50/SbcC-type AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02023155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUU2"/>
    <dbReference type="STRING" id="36166.T1GUU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007513-RA">
        <property type="protein sequence ID" value="MESCA007513-PA"/>
        <property type="gene ID" value="MESCA007513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297246_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="12">
        <location>
            <begin position="76"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="23627" checksum="5F1A86D424694A0B" modified="2013-10-16" version="1">MDCFVLVWTGNGILFPIVPLEGIRKWENCSALRKNHLPRKRSADTQSVNDVSAKRSRLSQELANIPCSQRSGKIMRIHLTNFMCHNNLVVDFSERINFLVGSNGSGKSAVMAALVIGLGCKASATSRSRSLKSFIKNGESYADIEIYISNDGKDQYEPETYGRMIVVSRHLTASGANSIKIKSDKGNLISKKKEELSKICLYLNIQADNPVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV73</accession>
    <name>T1GV73_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02110535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV73"/>
    <dbReference type="STRING" id="36166.T1GV73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007658-RA">
        <property type="protein sequence ID" value="MESCA007658-PA"/>
        <property type="gene ID" value="MESCA007658"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_875195_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF8">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="6">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="318" mass="36111" checksum="2C93555ED2876770" modified="2013-10-16" version="1">MECEWPLFFIFMIIDGVFKNNSDQIEEYQNLLRKCMSCDENGDPVVAMYFAPDGHGSYIRSPKQSLFLWGQSMFIIAQLLTGGLLHINELDPIRRYLPSYNRPRKAGRYSAFQNGDPVVAMYFAPDGHGSYIRSPKQSLFLWGQSMFIIAQLLTGGLLHINELDPIRRYLPSYNRPRKAGRYSAFQGTATDLVVQIVLIAESQRLQAMMATYGIQTQTPHEVEPVQIWSSTELIKVYQHLGVNKKVGLTGRPGRPVGSLGTSKVYRICGMTVLCYPLIFEVSDFYLYRDMALLIDDIKTELQFVGSIGVFLVGQQFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV92</accession>
    <name>T1GV92_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV92"/>
    <dbReference type="STRING" id="36166.T1GV92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007682-RA">
        <property type="protein sequence ID" value="MESCA007682-PA"/>
        <property type="gene ID" value="MESCA007682"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294850_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052069">
        <property type="entry name" value="Ca-reg_mRNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962">
        <property type="entry name" value="CALCIUM-REGULATED HEAT STABLE PROTEIN CRHSP-24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962:SF1">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN CG9705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007682-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11320" checksum="F21C31D911992779" modified="2013-10-16" version="1">MSFVLLPFTLNPTELAAFPMARSLSMTSDGVLPMTSMSSAYASSDQICIEGEFVPRPGDEVKYRLCHIPPKMEKVQAIHCQIINLTPEVHHKWEDICPDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVH0</accession>
    <name>T1GVH0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GVH0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007774-RA">
        <property type="protein sequence ID" value="MESCA007774-PA"/>
        <property type="gene ID" value="MESCA007774"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046756_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12906">
        <property type="entry name" value="SPRY_SOCS1-2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000007">
        <property type="entry name" value="SPRY domain-containing SOCS box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF11">
        <property type="entry name" value="PROTEIN GUSTAVUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007774-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="29313" checksum="7FC9C29312CFFA81" modified="2013-10-16" version="1">NGNTGIGGGGGNNGGGGATGSNSNGSDSNTQAPFKPVIPRELAAEFIKPTRVDILLDMPPVCHETQLKYTWNCDDRSLNIFVKEEDKFTFHRHPVAQSTDCIRGKVGFTKGLHIWEIYWPTRQRGTHAVVGVATRDAPLHSVGYQSLVGSTDQSWGWDLGRNKLYHDSKNCSGTTYPAILKHDEAFLVPDKFLVALDMDEGTLSFIVDQQYLGVAFRGLKGKTLYPIVSAVWGHCEIAXXXXXXXXXXXXXXXXXXDTAKLQCITSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVL9</accession>
    <name>T1GVL9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyroglutamyl-peptidase I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02049885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049887">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049888">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049889">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049892">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049893">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02049894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVL9"/>
    <dbReference type="STRING" id="36166.T1GVL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007830-RA">
        <property type="protein sequence ID" value="MESCA007830-PA"/>
        <property type="gene ID" value="MESCA007830"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1717807_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007830-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17370" checksum="6AF1BCE42DC821BA" modified="2013-10-16" version="1">MNDDQKLIFVTGFGPFVGHEEVNASWEAVKRLPDELDQRYLLKKLEVPVTYKDVDKIVEDIWKQKPYLVVHIGIDSRDYIAIEQNAPENNFPGLDVNNHHLEDGKAVLTNEGKSDCKLCTKLDTTDIVKKVGDQVNGPKIKHSETIESFLCGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWQ3</accession>
    <name>T1GWQ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Helicase domino</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWQ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008231-RA">
        <property type="protein sequence ID" value="MESCA008231-PA"/>
        <property type="gene ID" value="MESCA008231"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_227215_0_0_1"/>
    <dbReference type="OMA" id="YGEDCRG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18003">
        <property type="entry name" value="DEXQc_SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.850:FF:000012">
        <property type="entry name" value="protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="385"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="782"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="1343"/>
            <end position="1497"/>
        </location>
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            <begin position="1"/>
            <end position="110"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="11">
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="602"/>
            <end position="732"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1676"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1812"/>
            <end position="1863"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2170"/>
            <end position="2200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2213"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2470"/>
            <end position="2490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2635"/>
            <end position="2662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2685"/>
            <end position="2722"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1537"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="37"/>
            <end position="56"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="535"/>
            <end position="549"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
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            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008231-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2722" mass="303503" checksum="D368002EA2C7EE72" modified="2013-10-16" version="1">MNKGDSAGGGHRLSPASPAVTDRRSTQQIANTSILNTSELNSKTSTTTSSTATTPTKFQEIISQQLHQKQHQQQTSSGSAVSSSTNSTIVSTSHSPSHTAQNLPSSNLTLQPQSTRNILVVTSTIPQSMTITQQSKDGISSPIVSVVNRPSSYISTTAASTTSIVVPSSTVTASVVNNPTHSQVKRYSENVEELYFLSIGGNMMDYPTWKKKPLPQQFNNFNKAYRLNALTKNEDQKQASSIQSSNLNNLPEGAEVKISGIGVTPVAVSKTLPASVVQLTQQGHIPGRPIGGRHGMVFGQIPVSPAIGNSSVPLVPDKSAAIPPITQSPNVANSPLSKSSSALEVFNNNGNGGSSDVTVKAKQEVHVLQRISELQREGLWTEKRLPKLQEPSRPKAHWDYLLEEMVWLAADFAQERKWKKNAAKKCSRMVQKYFQDRANAAQKAEKAQELHLKRVAAFIAREVKIFWSNVEKIVEYKHTTKLEEKRKKALDQHLNFIVDQTEKFSQQLVEGMNKTVNTAENSAAASLNSSRISSPKRENASDDEFRPGEDSQDDEETIAKAEEENNSDVKDEIEALQKESEMDLDDFLNELPKDYLANREKLIQQETLSKSDDDDEEAEGNDVDHEKEIDELNAEAEMSIDELIAKYNANPPPKVSSGTSSASRRRSTRLAPIDEPSVHMDIDSDDYSEEENDKTDEEEDDDDYLTADEDNKNESGENTGLKSLLEDDPEKGDDLLHDAAALAESIQPKGNTLSSTNVVTPVPFLLKHTLREYQHIGLDWLVTMCDRKLNGILADEMGLGKTIQTIALLAHLACEKGNWGPHLIVVPSSVILNWEMEFKKWCPGFKILTYYGNQKERKLKRVGWTKPNAFHICITSYKLSQRWQLLLNFQTEQRLLLTGTPLQNNLMELWSLMHFLMPHVFQSHREFKEWFSNPVSGMIEGNAEYNESIIKRLHKVLRPFLLRRLKSEVEKQMPQKYEHVVMCRLSKRQRYLYDDFMGRAKTQETLNSGNLLNVINVLMQLRKVCNHPNLFETRPTISPFQMEGLSVKAPSIVCNIFKYDPFENIDLSALNLLLIQIETTLTAYVSYKIKRLQAPRKLIEEIDSNSTFTPKCPKGDYKFYVRVKDTKTSETMPPILILLANINPLNKTVLLDNNSLVNALAQELKSPIDVKMETEAETSLEINDNTKNMSTENDSEFNFKIYSEQRKFERTQNLMIQASINKRRCAATPIYGSDCRMSLENALSPIHKIDNSTWNSRSFTNCRRAQLSSYNWALERAIKSFEERTLGMSDIFDNFVLFVPSVLAPQPYLHARHLDPSKTSKERYREKMLRYHMSSKLSLLHPIISQMSTKFPDPRLIQYDCGKLQTLDRLLKTLKDGQHRVLIFTQMTKMLDVLEAFLNFHGHIYLRLDANFCVHSVDQKWRCWFNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRDVHIYRLISEKTIEENILKKANQKRMLGDLAIEGGNFTTAFFKTTTIQGLFEVQQENDASTRLAEVVESRSIQASEKVRDEKTSISTFENALAEAEDEQDVEAAKVAAAEAAADLDEFDENIPLEEIEKPEVSKVDQEMEQIVSQIGCDQKQEEELQKLEQIDNEGGDEYESDEDMMLTYSQEDASNQVKKRIKSSIPLSRSKKIINKRLTKVKEQHVRIIKNNKQSQISLKKKIVSSPKRPLRKRPNTISSSKMKASNGRDSVSNKKRKIDISNRRVTRSSPKKSSPNKSSCSNKKEPSISSNSDGDDEQNEQESEDERSFNNGRSSKNTNVTDDDETTMTECSLDVMLHDTTTNSTNCNKSDSENLSSKENPYSDYEEKEGGGPLLKNKLNNKLDVNSPRTRSRGSVKINLWTLDESPILPSRNSVHNNKKDSSSNLDDVNELISTPKIPNKTKIMLSSSIWISNNGFEEMPLWCPPTPPQNDNDLSLFDHPSAAIAKIRRDLKSQRYRGVFKPNIQVPGLKQQINQKAMVEPEGTPEWGIFEEVAVLHCLQNLQGLPLNLMLLSPGHTPNWDLVAETVNLYSKTYRPPGHCRAKFLAHIVPKEEGKLIDSPKKQKKLKLSSKPYLKGGLKMLKSSQQFSSDNNASYTRLMKNRFDAIKAAYLQKAPPPKRKFSTPSSTNLKHAEVLCSFGITSYDAPMSPTTIATRKAEKLREKHHHHHQQQQCSNNSQQISVQQSTPTAIIVQQSSSPQVTQILQRSQSTSSNSSGGTPTTSSGIQVHHHPSGQIVKAIVTPQQSPQIQLQQGNGTAQIVSLQPQQSQTSSTMVQAIPTTQVVSVSQLTTVGTVLTTSAGLQSTGSVTTLNAPGIRTQRIVTGPGSIQEVVLQQRPNTQSLQQSGKITSSQPTAQILYRQQPSRNQIKILQAQPGQGTTLVNTSGAINIVPATTQTMQVQGQKVTVASSAAGQPTQSIVTSVATVQVAQGQSRPQFIKQVGTKVGRADNEMLLVKRQPASQSQNTQVSSSGSTQVNLGQQQQQQQIATIVKTATGNSANITTSGNMLSQIKPSGQIKVMGNQTQMRSLQLQQQPLYQRKVGSAVTVQQIQQVIRHGAPGTLQTGGQIVLGKTRVIPVSLASQPNARQAIQVVSTGPSGQTINANNLRTHVASTQNISGTIKVTSSNASQQQQTQQSTQSHQSRQNSSPVRIQTGSNLVAVAVQQILPPQTSGQSIELQNVQQQQQPQQQASQSTTSTSSQQTTQQQPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWT8</accession>
    <name>T1GWT8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein heavy chain region D6 P-loop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02031239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWT8"/>
    <dbReference type="STRING" id="36166.T1GWT8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008269-RA">
        <property type="protein sequence ID" value="MESCA008269-PA"/>
        <property type="gene ID" value="MESCA008269"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1606796_0_0_1"/>
    <dbReference type="OMA" id="RIFKAIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000153">
        <property type="entry name" value="Dynein axonemal heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF63">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="1">
        <location>
            <begin position="56"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008269-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="166" mass="18818" checksum="2054FF6827D69CE1" modified="2013-10-16" version="1">MFHVLENSTSNAIHSKCFRIDRIFKAINEYITDIMGEYYITPPVISFVNIYEQTTCTIPVCFILSPGSDPTNELMKLAEQYGGGMGNFSHISLGQGQEKAAINLLNVSVRQGMWLMLQNGHLLISFIRSLEKQLEKIENPHPNFRLWITTDPTPTFPIGILQQSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXA1</accession>
    <name>T1GXA1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008443-RA">
        <property type="protein sequence ID" value="MESCA008443-PA"/>
        <property type="gene ID" value="MESCA008443"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1791696_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21253">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21253:SF0">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588581" evidence="1">
        <location>
            <begin position="25"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008443-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="145" mass="16434" checksum="79AC8171A5BE5810" modified="2013-10-16" version="1">MFLNKIAAICLITFTTFITSFTLALEQTSCNCTEYKQPFQSLVKRRKRRGIIFPTGSTILFTHAITKVIIGATPKGLSISYEMDVYFPLPDTLEGLYPKKLLKKSKPKPTPVMVEVPKPMEVNRTYLEGPLYGNYVDDSFLDIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXE5</accession>
    <name>T1GXE5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase</fullName>
            <ecNumber evidence="5">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02376442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXE5"/>
    <dbReference type="STRING" id="36166.T1GXE5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008493-RA">
        <property type="protein sequence ID" value="MESCA008493-PA"/>
        <property type="gene ID" value="MESCA008493"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_3_2_1"/>
    <dbReference type="OMA" id="QCQNPQI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008493-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="165" mass="18482" checksum="FE4EDB7830544AF8" modified="2013-10-16" version="1">MAGSGKTTFVQKLTQLSFDKYKPYVINLDPACKETPYHANIDIRDTVNYKEVMKQYCLGPNGGIVTSLNLFSTKFDKVLNLIEKAGKQEHKYTIIDTPGQIEVFTWSASGTIITEALATMFPTVIIYVMDVVRSTSPTTFMSNMLYACSILYKAKLPFIIALNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXM2</accession>
    <name>T1GXM2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXM2"/>
    <dbReference type="STRING" id="36166.T1GXM2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008575-RA">
        <property type="protein sequence ID" value="MESCA008575-PA"/>
        <property type="gene ID" value="MESCA008575"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1350263_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009838">
        <property type="term" value="P:abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044878">
        <property type="term" value="P:mitotic cytokinesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603">
        <property type="entry name" value="ABSCISSION/NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603:SF1">
        <property type="entry name" value="ABSCISSION_NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008575-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="203" mass="23060" checksum="3DB2D4BDA9B92534" modified="2013-10-16" version="1">MKLKVCPRCFEKLESQQKAEQLLPGVQKFTLSQISSVPGNTIFDVEDPSSEDLGPVLQPVQPNIKPEQEDDIISKNLDSVLSKRLQNLKSEDNKNLPTDEDISTRLADLKGMPQKNYNYSDLLVSKDHRTEKEKIDDLLKQFVDETAIDSNASRPSSNPIDEIEKRLAALRGTDTNEMIQLQVIMKMKLKMMMKLLPKLSTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXS7</accession>
    <name>T1GXS7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tubulin/FtsZ 2-layer sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02118353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXS7"/>
    <dbReference type="STRING" id="36166.T1GXS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008632-RA">
        <property type="protein sequence ID" value="MESCA008632-PA"/>
        <property type="gene ID" value="MESCA008632"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_6_0_1"/>
    <dbReference type="OMA" id="ACEDLCS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="7">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008632-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="21789" checksum="74FAB0FB5F6F340D" modified="2013-10-16" version="1">MQTNLIPYPRLHFTVLSYAPIVSACRSQLSNYSVREITVGSFEPSNQLVKCSPTDGKYMSCVLLYRGDVSPTDINQSIYDIKSRRGIQFVEWSPTGFKIGVNYMPPTYVPGGDLAPTNKAVCLASNTTAIRRPLCRVIDKFSKLFQRRAFVHHYVGEGLEEATIAEACEDLCSLVGDYTEVNSDAVNDDDQNGEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXZ7</accession>
    <name>T1GXZ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXZ7"/>
    <dbReference type="STRING" id="36166.T1GXZ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008712-RA">
        <property type="protein sequence ID" value="MESCA008712-PA"/>
        <property type="gene ID" value="MESCA008712"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_971706_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008712-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="287" mass="31051" checksum="D6DF884158C17567" modified="2013-10-16" version="1">PSSYITPASVFNTLAVDVDAEKNAGKEIPLKTVKLPASEILKLTRYTQNFDPCSSSSSTIAASASQSTLGSDTGYQTLSKTPPNLASYLESRKNSLPRQLSSESGTNMNLTGSLDRRPIVPPRTNLPGSQMGSQISTPRSKGGSLERNQSMSGAYDMMRSRVYRGGSLERNPSNGVTVARSGSLERNPNYLAYRTQMKANIENEPVQEEIYDFGGAHLKSCASIALNKGIAKGMIPHGTQLPTETPQPQLQPQQHSNNPFLQSQPQQPPTVMYNIMSMSQPNSLGMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYC3</accession>
    <name>T1GYC3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein tyrosine phosphatase type IVA 3</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Protein-tyrosine phosphatase 4a3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02186270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYC3"/>
    <dbReference type="STRING" id="36166.T1GYC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008847-RA">
        <property type="protein sequence ID" value="MESCA008847-PA"/>
        <property type="gene ID" value="MESCA008847"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175941_0_0_1"/>
    <dbReference type="OMA" id="RFLITHT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000105">
        <property type="entry name" value="Protein tyrosine phosphatase type IVA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="18">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9271" checksum="231738ED64D583AB" modified="2013-10-16" version="1">MNYQMSPEACVAVHCVAGLGRAPVLVALALIELGLKYEDAVEMIRDKRRGAINAKQLQYLQRYRPKSRLKQRNGHKNSCCVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYJ7</accession>
    <name>T1GYJ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008926-RA">
        <property type="protein sequence ID" value="MESCA008926-PA"/>
        <property type="gene ID" value="MESCA008926"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3430067_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008926-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="19" mass="2069" checksum="A544476367CBB7F2" modified="2013-10-16" version="1">MFSTPKKLTSEVSNKGTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYL3</accession>
    <name>T1GYL3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYL3"/>
    <dbReference type="STRING" id="36166.T1GYL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008946-RA">
        <property type="protein sequence ID" value="MESCA008946-PA"/>
        <property type="gene ID" value="MESCA008946"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514289_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000058">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF23">
        <property type="entry name" value="NEURAL-CADHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588614" description="Cadherin domain-containing protein" evidence="12">
        <location>
            <begin position="20"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="66"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008946-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="20426" checksum="17BE864E62BB80F6" modified="2013-10-16" version="1">MEWRRRFLVLLCLLSATSSSVEKRDSNSIYGQQHLRLTNNPMEFGWNQKDNRKPAFKNCLGYAPVVKEEQDKGTFVIQVEADDPDEGNNITYDLITSASERAKFEVNSKTGQIYTNHIFDRDEPSLEKSVYVTVKATDDGVPPLDDVCTFKVTIEDINDNPPYFIKAKYDERMAEDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ15</accession>
    <name>T1GZ15_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="EMBL" id="CAQQ02389143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ15"/>
    <dbReference type="STRING" id="36166.T1GZ15"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009106-RA">
        <property type="protein sequence ID" value="MESCA009106-PA"/>
        <property type="gene ID" value="MESCA009106"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149068_0_0_1"/>
    <dbReference type="OMA" id="WISMRIQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF69">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009106-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="17294" checksum="7967834B7F10D645" modified="2013-10-16" version="1">MVKEEEFLFPFNGFQFTVSIILVYLLFVLKIGKIYMKNRSPYKLKKFIVAYNIFQIAACLLVIKAIWSDEKAPPSRYFSQCQVAEYMNKWSYLYKFNVGIYLLKCSEMLETIIFVLRKKWRQVSFLHVFHHCATLTLAFLAGYCGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ52</accession>
    <name>T1GZ52_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080031">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009146-RA">
        <property type="protein sequence ID" value="MESCA009146-PA"/>
        <property type="gene ID" value="MESCA009146"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3413309_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009146-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="28" mass="3455" checksum="66E5E483F35DA493" modified="2013-10-16" version="1">MPYQPFYQYYSVPMQNVPTIWPQNYQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZD1</accession>
    <name>T1GZD1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZD1"/>
    <dbReference type="STRING" id="36166.T1GZD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009230-RA">
        <property type="protein sequence ID" value="MESCA009230-PA"/>
        <property type="gene ID" value="MESCA009230"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022631_2_0_1"/>
    <dbReference type="OMA" id="NTKYGIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058698">
        <property type="entry name" value="CUB_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33236:SF5">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33236">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 122 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26080">
        <property type="entry name" value="CUB_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009230-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="32322" checksum="1B9F54B2F77D70D3" modified="2013-10-16" version="1">MECGETSDFPVSYFFNTNYPSTYDGGGKCILYSEAYHKEICQLRIDFLSFSLAPPNGDGYCKIDALTISGLAKGTQIPQLCGENSGQHIYVDYDGRQPIILTFQTTDSYVFKRRWQLQLTQIPCNSVERAPSGCLQYYNSPNGTVQSFNYDAAGSAMMNSIGVSGTRHLANLKYKICVKQHLDRCSIEWAQVKSDRFSFTMTDDTSSVDPSLLGTMYVQSQNCRTDYVSIASPEQNGKKLTSDRFCGLGLLPTISFSKPFGIVVVTDGDESLDMGNRGFYLTYKQRQCPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZR0</accession>
    <name>T1GZR0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZR0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009364-RA">
        <property type="protein sequence ID" value="MESCA009364-PA"/>
        <property type="gene ID" value="MESCA009364"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2335990_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009364-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="10999" checksum="7692ADB3D8A3F056" modified="2013-10-16" version="1">MNKAVITTVDRRSISAKHNTENNRSPSIPKSYTISCSSTSLAFPLIASLRLATSRTSGKLTFLLIFNRNCSFSMSFSMPLASRSKFMVKLLTYPWEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1F8</accession>
    <name>T1H1F8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1F8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010017-RA">
        <property type="protein sequence ID" value="MESCA010017-PA"/>
        <property type="gene ID" value="MESCA010017"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_222192_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010017-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="20" mass="2351" checksum="D682BF6EDD30FDE1" modified="2013-10-16" version="1">MQDLKVLLEAKKLKVKIKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2J7</accession>
    <name>T1H2J7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2J7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010445-RA">
        <property type="protein sequence ID" value="MESCA010445-PA"/>
        <property type="gene ID" value="MESCA010445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1497934_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588694" evidence="2">
        <location>
            <begin position="18"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="180" mass="20797" checksum="0154C05EF7BB5F49" modified="2013-10-16" version="1">MWLKILFVFYILEVISGSQKLDTSDGKTKNSIHQENGQNGGGVWVPGPTPGTWIFWPHGHNFTGIPENNNTNAGNINPEQNPSPKPNVVTEIPGSNDPNNDKLKRESEWEKIKIKKAELEKRQTQRCNSIQELEDLEIKIKTRWEEREIENETKTKCRARKRALLEKKLRKKCGIHFIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2M2</accession>
    <name>T1H2M2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02382465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02382466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2M2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010472-RA">
        <property type="protein sequence ID" value="MESCA010472-PA"/>
        <property type="gene ID" value="MESCA010472"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576571_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010472-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9019" checksum="D6086BA0AD36DA61" modified="2025-10-08" version="2">MGITQRDFFFVQTLPNIRTTFTLIPLTVNISLTHSLLSHRNSQTEPPLKSNANAVDKRAMCNATIPRWACISLNAFTGCIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2R8</accession>
    <name>T1H2R8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010522-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="7703" checksum="E765FBAE418DD3CE" modified="2013-10-16" version="1">MTQGQRKAHEIQNASQNIDLESPRISAANSSLHIDNNFSLENNQQGNSSLEDGMEIPMGSNQSPTNAHSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2R9</accession>
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        <recommendedName>
            <fullName evidence="1">CCDC22 N-terminal domain-containing protein</fullName>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21674"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010523-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14032" checksum="C1F12512C071F0CF" modified="2013-10-16" version="1">MEEVDNIIIHSLKQIGCHIGEDVKNLTNFPPELLVETVASCIQLIDPSLSLPKSLPSNLAQRFSTTATLAEACKTLGFRGDIGYQTFLYSNVSEVRRVYMFLIEKLPKDDIQEELSVGVIVSILLH</sequence>
</entry>
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        <source>
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        </source>
    </evidence>
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</entry>
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</entry>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
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</entry>
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            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02199">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00162">
        <property type="entry name" value="SAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51110">
        <property type="entry name" value="SAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Saposin B-type" evidence="7">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin A-type" evidence="8">
        <location>
            <begin position="120"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010905-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18503" checksum="D2D76E793C3305F6" modified="2013-10-16" version="1">MLDSVPPKEICSRMNLCAMNDISNDILECGVCQGVSAFLVSHKDTELTKNEMKDLTCDSFPSKYFDKCTDIIEYYSQSIQNLIKSNISENEICTKIGKCFDKEGKHSYQVVMNSHKSKRTLVGDNECTFGPSHWCKDKETAKKCDLVDYCEKIWKKKENKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3V9</accession>
    <name>T1H3V9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3V9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010954-RA">
        <property type="protein sequence ID" value="MESCA010954-PA"/>
        <property type="gene ID" value="MESCA010954"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515241_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010954-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="10095" checksum="6CC5A3071C6D0295" modified="2013-10-16" version="1">MFYPEGQGILKLERGRPPVTRREEFTNRMLRECLQESRILVECACCKSAKRFKRTLYTRIMRNGLSFEEKGRTNGTKHTYYLENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4G4</accession>
    <name>T1H4G4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4G4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011173-RA">
        <property type="protein sequence ID" value="MESCA011173-PA"/>
        <property type="gene ID" value="MESCA011173"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1580305_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011173-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="169" mass="19792" checksum="8EDBE2C3285BEDEA" modified="2013-10-16" version="1">MYNLPFWSNYFGKRLDPNCDRGSNQTAEAFFNMAKNLQANREKHIRPVKFISCIQTIIEGGIRKTCESSIGITATSIEKKMLKIKSPTAKTPRSSRRETNNSPRCSTTPRSSTRKRIYEGDIEEKEELNIQEEWGTKEDHKKRMTYYNAKRMKQMANIFHNKMDEQDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5R2</accession>
    <name>T1H5R2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5R2"/>
    <dbReference type="STRING" id="36166.T1H5R2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012045-RA">
        <property type="protein sequence ID" value="MESCA012045-PA"/>
        <property type="gene ID" value="MESCA012045"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_1_0_1"/>
    <dbReference type="OMA" id="AKWQPCN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012045-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20554" checksum="61D850F7C34B94A0" modified="2013-10-16" version="1">SVANSLRRVFIAETPTLAIDWIQLEANSTVLSDEFIAHRIGLIPLISDDVVERLQYTRDCTCRDFCPDCSVEFTLDVKCTEEQTRHVTTADLISSNPKVVPASSKNEYGESSDEILIIKLRKGQELKLKAYAKKGFGKEHAKWNPTAGVCFEYDPDNAMRHTLYPIPHEWPKSEHTELEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5U6</accession>
    <name>T1H5U6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neurotransmitter-gated ion-channel ligand-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5U6"/>
    <dbReference type="STRING" id="36166.T1H5U6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012079-RA">
        <property type="protein sequence ID" value="MESCA012079-PA"/>
        <property type="gene ID" value="MESCA012079"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2765153_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012079-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8411" checksum="4367D9274FDDF62F" modified="2013-10-16" version="1">FSIFVVDINSINVEDMDFRVDMFIHQKWTESRLNISEDIFEEGDDYVTLLPDFFLDLWQPDPYFLNSKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6F6</accession>
    <name>T1H6F6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit E</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6F6"/>
    <dbReference type="STRING" id="36166.T1H6F6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012289-RA">
        <property type="protein sequence ID" value="MESCA012289-PA"/>
        <property type="gene ID" value="MESCA012289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073641_2_0_1"/>
    <dbReference type="OMA" id="QHMMAFI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21671" checksum="33C5A5DF310780B6" modified="2013-10-16" version="1">NIEKGRLVQQQRLKIMEYYEKKEKQVELQKKIQSSNMLNQARLKVLKVREDHVRSVLDEARRRLGEVTKNQAEYRQILQKLIVQALYQTMEKNVTLRIRRVDENLIREIIPDAAELYKSNIGKDININIDTDHPPNTCGGVEVIALNGRVKVPNTLESRLELICQQLVPETRNALFGVNPNRKFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6J6</accession>
    <name>T1H6J6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6J6"/>
    <dbReference type="STRING" id="36166.T1H6J6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012329-RA">
        <property type="protein sequence ID" value="MESCA012329-PA"/>
        <property type="gene ID" value="MESCA012329"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901485_0_0_1"/>
    <dbReference type="OMA" id="INGKGEQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="16"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012329-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="14910" checksum="725BF541594B3E25" modified="2013-10-16" version="1">GGYAPPPGAFPPQNSGVPPQVQQWFQAVDRDRSGKINSQELKAALINGKGEQFSDNSCKLMIQMGFRFSPEFINFLVKKLDPTKHSEISVDNFIVVCVQVQRFTEAFRVRDTEQKGQITIGFEDFLSIAIGSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6S8</accession>
    <name>T1H6S8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6S8"/>
    <dbReference type="STRING" id="36166.T1H6S8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012411-RA">
        <property type="protein sequence ID" value="MESCA012411-PA"/>
        <property type="gene ID" value="MESCA012411"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030301_1_0_1"/>
    <dbReference type="OMA" id="PWSAHHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF3">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OAR" evidence="3">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012411-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="18570" checksum="E6B3EEC347962FF2" modified="2013-10-16" version="1">MNAAVAAAEFKTGFGTQFMQPFADDALYSSYSPYNNWTKVPSPLGTKAPFPWPVNPLGSVVSSNHHQNSVNCFNTSASSVAVSMNSSNMLPGSMGGSAGGSFWATCPYTTPTNPYMYHTRAAEPCNAMSSSIATLRLKAKQHTSGFSSPYTSHSPVSRSNSVGLSACQYTGVTDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6W9</accession>
    <name>T1H6W9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6W9"/>
    <dbReference type="STRING" id="36166.T1H6W9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012451-RA">
        <property type="protein sequence ID" value="MESCA012451-PA"/>
        <property type="gene ID" value="MESCA012451"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2612876_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000502">
        <property type="entry name" value="Actin-Related Proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012451-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9028" checksum="C08D7E546923341D" modified="2013-10-16" version="1">MIKVGFAGDNIPKCRFPNYIGRPKHTRVMAGALEGDIFVGPKAEEHRGLLSIKYPMEHGMIWREFGTTFNREKAAEIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H716</accession>
    <name>T1H716_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H716"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012498-RA">
        <property type="protein sequence ID" value="MESCA012498-PA"/>
        <property type="gene ID" value="MESCA012498"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551483_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF23">
        <property type="entry name" value="FURIN-LIKE PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="6">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="19097" checksum="92DD8E0355602D9C" modified="2013-10-16" version="1">FILFSLQIGSLKGYYLFSHRHISKRSVTVSEPHHGALNSEPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNGGAKTGLDMNVIPAWQKGYTGKGVVVSILDDGIQTNHPDLAQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H750</accession>
    <name>T1H750_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H750"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012532-RA">
        <property type="protein sequence ID" value="MESCA012532-PA"/>
        <property type="gene ID" value="MESCA012532"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329237_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22255">
        <property type="entry name" value="LP06548P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22255:SF9">
        <property type="entry name" value="LP06548P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23459" checksum="99647584219990A3" modified="2013-10-16" version="1">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGSRIMTLRKPTPAERCDFPSWFKGPRHWHALMGNSLYNYHQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIQSGKPANRLEDACAPDYFDFNTTPFITLLAKDPDPQICPLEGLYFLHGAIGPPYVSSRHKRNHNGKHNHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H782</accession>
    <name>T1H782_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H782"/>
    <dbReference type="STRING" id="36166.T1H782"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012620-RA">
        <property type="protein sequence ID" value="MESCA012620-PA"/>
        <property type="gene ID" value="MESCA012620"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059592_3_1_1"/>
    <dbReference type="OMA" id="RTSKAWV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588776" description="Protein YIPF" evidence="7">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012620-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16083" checksum="C1552CFC000D6B16" modified="2013-10-16" version="1">DLWGPLVLCTFLATCLQSSADNQYEDGPEFAQVFVIVWIGAAIVTLNSKLLGGNLSFFQSVCVLGYCLTPIAVALLVCRVILLSTQTKIMFFLRLLTTIFGFSWSTYAAYIFLGDSQPAHRKPLAVYPIFLFFFIISWLVISHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA99</accession>
    <name>T1GA99_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02114728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA000149-RA">
        <property type="protein sequence ID" value="MESCA000149-PA"/>
        <property type="gene ID" value="MESCA000149"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1322247_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000149-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="23599" checksum="E8378716DA578FD1" modified="2013-10-16" version="1">MCLFEIEDCNGASKLRKVLAKKKVIPVYIRKSDGSWAENSEEMSNIFMDTHFPELVAVDCETXSWAENSEEMSNILMDTHFPESVAVDYETENILETHEGVAEYCSCFQIFGRKNLWTLKFGIQLTRIFYPNNSMHIGELLKNRGGTIVGYADDGVLIARSTFPDTLTLRVVSVCTKTNGLGVNTTKTDLVFFTIRHRISLFRLPILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAH6</accession>
    <name>T1GAH6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000233-RA">
        <property type="protein sequence ID" value="MESCA000233-PA"/>
        <property type="gene ID" value="MESCA000233"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545206_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000233-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9598" checksum="1B67522E322FA6C5" modified="2013-10-16" version="1">MKRQTDPDWLLFIFSYIDSVLFPVAKALLNQPPFTHESMNLCCVCGFLLFSTTNRQPISKYLCIILEKPKNVKSTLNKRNETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAN0</accession>
    <name>T1GAN0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02070792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAN0"/>
    <dbReference type="STRING" id="36166.T1GAN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000289-RA">
        <property type="protein sequence ID" value="MESCA000289-PA"/>
        <property type="gene ID" value="MESCA000289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2225874_0_0_1"/>
    <dbReference type="OMA" id="HTHHGLH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="13617" checksum="F5BC1CA31CB8397B" modified="2013-10-16" version="1">MERSSITGLDNMSDSGLCLQDLVGNTTNTNGSGDGSSSTTGPSRGGSTNNSATGSSNSSADHLHSHHNMHDTSSTVSVSPTVSGILPGVGLSHIHHSSQDLNNHHGHHGISISSHTPNTVLHEPLEKLKKEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAW4</accession>
    <name>T1GAW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02167940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02167942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000379-RA">
        <property type="protein sequence ID" value="MESCA000379-PA"/>
        <property type="gene ID" value="MESCA000379"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856326_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000379-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7322" checksum="0781C393DBE9DF18" modified="2013-10-16" version="1">GNKARLSYPNWNGYIPTNSIGLDNSGFFSILLGIRRTICMIELREGFENIFGVLLVQGKIIETLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBG4</accession>
    <name>T1GBG4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC-2 type transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02104830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBG4"/>
    <dbReference type="STRING" id="36166.T1GBG4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000596-RA">
        <property type="protein sequence ID" value="MESCA000596-PA"/>
        <property type="gene ID" value="MESCA000596"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052287_0_0_1"/>
    <dbReference type="OMA" id="YIDITIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000596-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13899" checksum="DCD273C78B408E65" modified="2013-10-16" version="1">MAISYYLTGNFIADMYRFYYFLILGSLATLAAQAWGFFVGATMPMKIAVFIGPILAVLFSVFGFCTRYIDITIMFRWMWHISYFRSGFHAILNSVYGDNRPFLDCPESAIYCHFKSPRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBQ8</accession>
    <name>T1GBQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02128616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02128618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBQ8"/>
    <dbReference type="STRING" id="36166.T1GBQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000695-RA">
        <property type="protein sequence ID" value="MESCA000695-PA"/>
        <property type="gene ID" value="MESCA000695"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798650_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000695-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="15960" checksum="4EE8EC0C0F1D1997" modified="2013-10-16" version="1">MLIFKEDIVLNGYTCIVPSLGVGNVSQLSTDLIISTLKLKQVATGFHKALIPIFGPSAFQHEPDSFTNALNVYIDSSKKLLAFQIRSPLIGKHTKDFLNNLVEFLKKSQVSKIFADHKKEFSASSSPKFSLVKISQRYGSVVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBY3</accession>
    <name>T1GBY3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBY3"/>
    <dbReference type="STRING" id="36166.T1GBY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000774-RA">
        <property type="protein sequence ID" value="MESCA000774-PA"/>
        <property type="gene ID" value="MESCA000774"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1148348_0_0_1"/>
    <dbReference type="OMA" id="TICTIDH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097149">
        <property type="term" value="C:centralspindlin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04382">
        <property type="entry name" value="RhoGAP_MgcRacGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199:SF3">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="2"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000774-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="27806" checksum="9A882C232ECAEA7F" modified="2013-10-16" version="1">MGYISEYVPKVAPFIPALIVHCVVEIESRGLNEVGIYRISGLEREVKALKEMFLKSQTISDLSSIDIHVLCGCVKDFLRSLKEPLIPQLSWKDFHRAIENIDDADESKFQMLKVIEDLPQANRDTLSFLVLHFQRISECEEVKMPLDNLAKIFGPTIVGFSSPESNQSTIFRELPIQFNIMKSLMSIPMEYWFQFVDVRCFSAEKTLSIHSKSRQTNPLYPLYASPLKGSIRRKKILYGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC03</accession>
    <name>T1GC03_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC03"/>
    <dbReference type="STRING" id="36166.T1GC03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000797-RA">
        <property type="protein sequence ID" value="MESCA000797-PA"/>
        <property type="gene ID" value="MESCA000797"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560900_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF26">
        <property type="entry name" value="ZINC FINGER PROTEIN 423 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000797-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9498" checksum="B8B42ACDF84F59AD" modified="2013-10-16" version="1">MVSKANSLQNHQLTVHSSEAKRFPCPFEGCQFVTDKAYSLKTHTWTHKEERDFPCTFEGCEYRGKTKAALWKQCTSLAQNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC47</accession>
    <name>T1GC47_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC47"/>
    <dbReference type="STRING" id="36166.T1GC47"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000845-RA">
        <property type="protein sequence ID" value="MESCA000845-PA"/>
        <property type="gene ID" value="MESCA000845"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1074382_0_0_1"/>
    <dbReference type="OMA" id="YIRDAWK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000845-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="25682" checksum="52DC543249A579F7" modified="2013-10-16" version="1">MGAYRYMQELYRKKQSDVMRYLLRIRVWQYRQLTKLHRAPRPSRPDKARRLGYRSKQGYCIYRIRVRRGGRKRPVPKGCTYGKPKSHGVNELKPYRCLQSIAEERVGRRLGGLRVLNSYWVAQDASYKYFEVIMVDIHHNAIRRDPKINWLCKAVHKHREMPVSLPLERAPVVLERDIDTHKPLEDLAVLPGNARTNLTCTENDKKFMFLLIYVMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCY4</accession>
    <name>T1GCY4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IST1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Charged multivesicular body protein 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Interacts with SPART (via MIT domain); leading to the recruitment of SPART to midbodies. Interacts with SPAST.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02078381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02078383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCY4"/>
    <dbReference type="STRING" id="36166.T1GCY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001157-RA">
        <property type="protein sequence ID" value="MESCA001157-PA"/>
        <property type="gene ID" value="MESCA001157"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037652_0_0_1"/>
    <dbReference type="OMA" id="YQPFPNI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000001">
        <property type="entry name" value="IST1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="192"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="263"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001157-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="371" mass="41554" checksum="F9057EF7F890E5D8" modified="2013-10-16" version="1">MRIFKHPTNFCEQAQTPEKKKAELTQKSRKEIADYISGGKVERARIRVEYIIREDYLVEAMEVVEMYCDLLLARFGLITQMKELDDGIAEAVCSLVWVAPRLQNDVSELKIISEIFIKKYGPNFGEHCRTVTGDHRVSEKLMHKLSIQAPPKLLVEKYMIEIAKNYNIEYEADPQIMKSDQKEEDEDGFHWISSSSTLPQMPDPPSYKPFNYPPPSGGGGGGGAAQAAQGGPSAPQPFNYNIPPNVSNEKKDLNINTDFLAAEGASNIPPVSTNKESPDDNAQTAPPPDPFESLPPRYSTVVTNNNLQANDKPKPQPRAKIPDPAPRPMPNLPNVPLDLPDIPNDDEEKKDDDELDFDELNRRFQALKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCZ3</accession>
    <name>T1GCZ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">adenylate cyclase</fullName>
            <ecNumber evidence="3">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02156508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02156509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCZ3"/>
    <dbReference type="STRING" id="36166.T1GCZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001167-RA">
        <property type="protein sequence ID" value="MESCA001167-PA"/>
        <property type="gene ID" value="MESCA001167"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297244_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF30">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="12">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001167-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="24435" checksum="25DB8A1892E01CCF" modified="2013-10-16" version="1">MGVTAAIIGIVHSILNELKQRIAFLEAKKSLEVNMKIEEHSQVQERLLLSVLPKYVADKVRMDMGVTKSDQFKKIYMSRHENVRYLQHKTNSPVDMRVGIHTGAILAGILGQRQWQFDVYSRDVELANKMESSGMAGRVHISEKTRLCLRDQFVVEPAYGEKREPALRLAGLTTYFITKVLVPFEERNRSLTEDHLAIDMQSEKEEGFKDMID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDW1</accession>
    <name>T1GDW1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02175305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDW1"/>
    <dbReference type="STRING" id="36166.T1GDW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001512-RA">
        <property type="protein sequence ID" value="MESCA001512-PA"/>
        <property type="gene ID" value="MESCA001512"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443371_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="8979" checksum="46759DA5D3CC2BC0" modified="2013-10-16" version="1">MNSYPSMGVAATTASNLAQFYQHAASAAAAGVGGPTDALGVGALAYTQPSAGQLGSASTDISKYPWMSLVAEVLYHKSGAGVSPQSLRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE68</accession>
    <name>T1GE68_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02103641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001626-RA">
        <property type="protein sequence ID" value="MESCA001626-PA"/>
        <property type="gene ID" value="MESCA001626"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2489745_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001626-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9514" checksum="9A4850D0282C8C27" modified="2013-10-16" version="1">MKPPLGSGMKGNPIHRISKMKTFIFLTFLIGLFGISEPNERQKRFFGETFGHLLNGATSAFGSVIEKVFPGSEKLSNSKPAEILSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEV1</accession>
    <name>T1GEV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEV1"/>
    <dbReference type="STRING" id="36166.T1GEV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001872-RA">
        <property type="protein sequence ID" value="MESCA001872-PA"/>
        <property type="gene ID" value="MESCA001872"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1808409_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001872-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="143" mass="16599" checksum="14F960003EBC8C82" modified="2013-10-16" version="1">MNHEVSHDKHEEEQVTNTSSIALREQFPNKRKKSDDATRNASKFSLDISKKMRKPGFGLGLNRNHYFIFGFLLGLVLSFYIPENVWDLVQSECPQLALENNLIEKFGEDFEPHLNLINKPLAAKKPKPTRAYNHRHCLLRLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG19</accession>
    <name>T1GG19_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG19"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002324-RA">
        <property type="protein sequence ID" value="MESCA002324-PA"/>
        <property type="gene ID" value="MESCA002324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608761_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9414" checksum="E371F5D3318DCA12" modified="2013-10-16" version="1">MLPRKRLTMKSRTSSANCEIKHILPSLLLILNYGKGDHLQRKNGKAHPTRHQKWQWSKTNKFCSIEEHRMHQVLTWIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG73</accession>
    <name>T1GG73_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="7">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02391222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG73"/>
    <dbReference type="STRING" id="36166.T1GG73"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002383-RA">
        <property type="protein sequence ID" value="MESCA002383-PA"/>
        <property type="gene ID" value="MESCA002383"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_845414_0_0_1"/>
    <dbReference type="OMA" id="WIAVGEN"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="8">
        <location>
            <begin position="176"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002383-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="329" mass="38434" checksum="ACE6D6590D4126F2" modified="2013-10-16" version="1">MFPYQPTQWQLFLSPQNCSRSHGSGGIEETSTPLFIWGSGIKNWRTFIESTEFVIVENNKVPLYTVEQAQIAPLMSSLIGQSPPMNNFGKLPFQLMNVSLEFQAESLSINAFQMISQYEKLMDQFEKGFFSKMLPTFEISKTNISGFYEDVRFHMKNQEFQKVITSSEKMIDLSLEGIEYFQTYYKNILLFCTVMTFLGWIFYLLQVLQRNLHFKLQQLFMKTIIFISIPIIIIFLQKIPLEIAFYILLPIVIWIAVGENFGLILKNVRTFEVQTFLLLIFGCEIVVLSFFKKEIISLGFIALSLKRTNLSQMTSFYQKLHFFIVYISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG94</accession>
    <name>T1GG94_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02041173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG94"/>
    <dbReference type="STRING" id="36166.T1GG94"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002406-RA">
        <property type="protein sequence ID" value="MESCA002406-PA"/>
        <property type="gene ID" value="MESCA002406"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123911_0_0_1"/>
    <dbReference type="OMA" id="LAGYNFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF78">
        <property type="entry name" value="ABC TRANSPORTER EXPRESSED IN TRACHEA, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002406-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12616" checksum="09FCA5FED91693D0" modified="2013-10-16" version="1">MKWKQNAARTRGKLSQFSQNNLCNGGLPKVQNNSPNGQKQQLTHLPQRPPVDIEFSNLTYSVHEQGKTFLGTAKKILKEVNGKFRNGELTAIMGPSGAGKSTLMNILAGYNFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGF8</accession>
    <name>T1GGF8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
            <location evidence="1 9">Extracellular space</location>
            <location evidence="1 9">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02094698">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGF8"/>
    <dbReference type="SMR" id="T1GGF8"/>
    <dbReference type="STRING" id="36166.T1GGF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002478-RA">
        <property type="protein sequence ID" value="MESCA002478-PA"/>
        <property type="gene ID" value="MESCA002478"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1201024_0_0_1"/>
    <dbReference type="OMA" id="WFMYSEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060560">
        <property type="term" value="P:developmental growth involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF91">
        <property type="entry name" value="PROTO-ONCOGENE WNT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002478-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="231" mass="26431" checksum="13F6D6669CEB93C1" modified="2013-10-16" version="1">MSRNVWFMYSENLLDAFSKFPCIGDNLKERFDGASRVMVSNSVRSNNNNNNDNKDNNGDSNSININNNENQIKAISVQSNAISSNSIPRNSMRSRGRGNKKNNRYHFQLKPYNPDLKPPSVKDLVYLEPSPGFCEKNSRQGIQGTHGRQCNDTSIGVDGCDLMCCGRGYRTEEVIVTEKCGCTFHWCCEVKCKNCKTKKTIHRCLKKKTFLMYISLFCYSNVVIFVNIYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGU0</accession>
    <name>T1GGU0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.</text>
    </comment>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. With POLR3H/RPC8 forms a mobile stalk that protrudes from Pol III core and functions primarily in transcription initiation. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGU0"/>
    <dbReference type="STRING" id="36166.T1GGU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002621-RA">
        <property type="protein sequence ID" value="MESCA002621-PA"/>
        <property type="gene ID" value="MESCA002621"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2161252_0_0_1"/>
    <dbReference type="OMA" id="STHAWAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002621-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="13121" checksum="63406DE32D9E93D2" modified="2013-10-16" version="1">MEIKNTCYSSLTNFEVLEALKAISKKDTKKKYGLRNLATITYETLQYLEETPVKSQTKESITDFLREVSQYKLTKNEILMIVNDPPSKPYTFNFWWKTAKRDSPKIKLIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH67</accession>
    <name>T1GH67_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02075711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH67"/>
    <dbReference type="STRING" id="36166.T1GH67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002754-RA">
        <property type="protein sequence ID" value="MESCA002754-PA"/>
        <property type="gene ID" value="MESCA002754"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1760869_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16907">
        <property type="entry name" value="YEATS_YEATS2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YEATS" evidence="3">
        <location>
            <begin position="28"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002754-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="17174" checksum="F44039B6D971E9D0" modified="2013-10-16" version="1">MDRKLEFIQLLELYLICKKSQISSLNSARCSNQTKHLIVVGNTSKYINLEDPSDSSTHKWLIYVQSKTSIPIENFVSKVKFYLHPSYSPNDIVEIVSPPFQIARRGWGEFPVRVQMFFHQHLRQNPLQLIHNLVLDKTLTGLQTMGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHB9</accession>
    <name>T1GHB9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000730">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHB9"/>
    <dbReference type="STRING" id="36166.T1GHB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002811-RA">
        <property type="protein sequence ID" value="MESCA002811-PA"/>
        <property type="gene ID" value="MESCA002811"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2229847_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF11">
        <property type="entry name" value="MITOCHONDRIAL COENZYME A DIPHOSPHATASE NUDT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="7"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002811-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="11725" checksum="7142BD98261FA52E" modified="2013-10-16" version="1">MKTKGSDKYAAVLIPLCLNSNGELSILYTRRSGNLRRHTRQISFPGGLRDHTDSSYEETALRETEEEIGLHKNRIKVLGNGALIRPPNTAAIMPIVGLIENLDLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHJ4</accession>
    <name>T1GHJ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein dpy-30 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394002">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHJ4"/>
    <dbReference type="STRING" id="36166.T1GHJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002898-RA">
        <property type="protein sequence ID" value="MESCA002898-PA"/>
        <property type="gene ID" value="MESCA002898"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135823_4_0_1"/>
    <dbReference type="OMA" id="CDEASEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000003">
        <property type="entry name" value="protein dpy-30 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037856">
        <property type="entry name" value="Sdc1/DPY30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356:SF16">
        <property type="entry name" value="DPY30 DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356">
        <property type="entry name" value="DPY30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002898-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8083" checksum="7AEBA1D3896AD9D6" modified="2013-10-16" version="1">MEKSETSAASQTLKKPRADLNSLPTRQYLDQTVAPILLQGLHTLARERPADPISFLAAYLLKNKGRCDEASEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHL9</accession>
    <name>T1GHL9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002926-RA">
        <property type="protein sequence ID" value="MESCA002926-PA"/>
        <property type="gene ID" value="MESCA002926"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2678093_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002926-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8669" checksum="93CC8D0D63F175D9" modified="2013-10-16" version="1">KINFKTFFLNQTFSRQSKVKSKISLKQVRRAHYNASTKAVEHKLGAITQPMMSLTAFGFMGLKYDLSVSFVTWMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI99</accession>
    <name>T1GI99_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003168-RA">
        <property type="protein sequence ID" value="MESCA003168-PA"/>
        <property type="gene ID" value="MESCA003168"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111178_2_0_1"/>
    <dbReference type="OMA" id="SRTMQFK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039715">
        <property type="entry name" value="ZCCHC10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13491">
        <property type="entry name" value="ZCCHC10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13491:SF0">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13917">
        <property type="entry name" value="zf-CCHC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15408" checksum="5760958411E618E4" modified="2013-10-16" version="1">MLARKEYQKKKQEDLAKAFPNGIKCQKCLEFGHWSYECKGKRKYLHRSSRTVHLKKTVEEKKLKLEEEAAAAAAGEEPKKSQKSKKKRTHSSSSSSSSSSSSGSSSSDSSEDSESDSSSSSSSSSSTSSSGSSSSSSNSGSESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIK7</accession>
    <name>T1GIK7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein KRI1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02131084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GIK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003287-RA">
        <property type="protein sequence ID" value="MESCA003287-PA"/>
        <property type="gene ID" value="MESCA003287"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009647_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="4">
        <location>
            <begin position="485"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="583"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="628"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003287-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="769" mass="90299" checksum="1C8FECB42B1743C8" modified="2013-10-16" version="1">MSSKFGKKQLFDEDSDQEEESLNLKTNKDYAKKFNSLREKELYKKLKEQDLENDGSGSDSSDSSTDEDEVNDPKFDEEFFKTLSSLKSKDPKIYDKESKFFNDSSSSDDEEDGESKSKKQKPMTLNDFERKVIVEKGGKYVDDDDESEEERPFVNKFKDQDLTYVQEQNKLKEEFSKAINEEDSDEETRRKRSLLSGSPDEKXXXXXXXXXXGDDALKDEDAKKSLAPLKEYWNKPTLTSREKFLRDYILTKGYADAEHDEIPTYEEIVETTEFEHKFNFRYEEPDDDFIKRYPRTIDNSVRKQDTKRKQKRQETKDRKLKEKEEKMQQIEMLKELKRKEIQEKIDKLKMVAGQDTIPFNPDELDEDFDPEAHDRRMQELFNDEYYQVDEGEEKPECDIDELKFEDWDNYEATEGNDDDEGGHCEDDDFNMDCDYDPKAAAEARKKSLQEELIENTTKKKRGRRNRFMEMIKSKKPVFDPEDEKTYSEYIDEYYKMDCEDIIGDTQCRFKYTECEPNDFGLTIEEAVSRNPSKPKRFPDSPGQEQGTNQWASLKKAVQIRPEHLEKKEQRLYKLKAKNEELKRKIFKSLYDPSSDEEEEPKKNTEVTLGGWEVTTNKKKKKKKKNKSSTETSETPENPPSTSKSDTLKRKAEPEQTQNDSQTFKKKKPNDGNNSEKLKNNKPNTEKSKPQNNNFKNKKPQNKNPFNKDGANGVSDDRLKAYGINPKKFHNQQKFGKNQKQKQSNFNGNKQNNPKKKPQTSNGAKKSKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIL9</accession>
    <name>T1GIL9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02048254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIL9"/>
    <dbReference type="STRING" id="36166.T1GIL9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003300-RA">
        <property type="protein sequence ID" value="MESCA003300-PA"/>
        <property type="gene ID" value="MESCA003300"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118380_0_0_1"/>
    <dbReference type="OMA" id="MEERHCA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF28">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MINIBRAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20529" checksum="E59A9205E061C609" modified="2013-10-16" version="1">MDIAIYQYIQSRFYRSPEVLLGIPYDLGIDMWSLGCILVEMHTGEPLFSGCNEVDQMNKIVEVLGMPPKYLLDGAHKTRKFFDKNLDGSFVLKKSPNGRKYKPAGTRKLHDILGVETGGPGGRRLDEAGHTVSDYLKFKDLILRMLDYDPKNRATPYYALQHNFFKRTSDEGTNTTSISSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIZ3</accession>
    <name>T1GIZ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CAQQ02392599">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIZ3"/>
    <dbReference type="STRING" id="36166.T1GIZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003430-RA">
        <property type="protein sequence ID" value="MESCA003430-PA"/>
        <property type="gene ID" value="MESCA003430"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036849_6_2_1"/>
    <dbReference type="OMA" id="MFTHISI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF349">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE BRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003430-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="28910" checksum="039C85F9ADA94D7F" modified="2013-10-16" version="1">MGMKDGPQRQQHAKNFENREAIRRTWGAQGLLNGVKIRTLFNLGRSSETSEKVDLEARTFKDIIQSSFTDTYKNLIFKTLMGLKYAFHSCSSSDYFVFVDDDAYVSVVNTLKALPKRSDTIFYSGNIIWKPSVFRNPFKKWYISIKEYPFNEYPNFVSGTFTMLTKYTLQLFWLGTFYTKPFQFDDVFLGIIAYKLRIPVTVNIDCFNEMLEYKGPESYKSVIVMHGYESCREMEKVWLECREAGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJI0</accession>
    <name>T1GJI0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJI0"/>
    <dbReference type="STRING" id="36166.T1GJI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003629-RA">
        <property type="protein sequence ID" value="MESCA003629-PA"/>
        <property type="gene ID" value="MESCA003629"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_807228_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="107"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003629-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="344" mass="40080" checksum="1A891EDF11CE8DFE" modified="2013-10-16" version="1">MITQAKRRTNVKQKLEESLRKEIARRSEDEGENKEVPPPEFEKDFEMVDMKKEEAPQEQPKPESVNIALQEPNYYMLPRIVDTIYVTGDIKEISSGLYHFALISTSGTLYTWGKNLEYQLGNGDGERKSISFPQIVEGITNPIHVECGADFTLVMMDDLTMKSFGGNANGQCAVPLTNTQETKSGRLFCLPTTKRLLRLEGKCVITPTEVNLPRPRINLDNEPIRHLKSLPKFKNTLSTLPMKKHHIKYCIYYLLQHFLDKKFEFSDCEQYFMNDLDYYLLELSFVLYLSNNNNFEFEQFFDEKFNNTNNILMDDANIQQDIFQLNYIDMIFENLTVKFKTVFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJN0</accession>
    <name>T1GJN0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003448">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003450">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJN0"/>
    <dbReference type="STRING" id="36166.T1GJN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003680-RA">
        <property type="protein sequence ID" value="MESCA003680-PA"/>
        <property type="gene ID" value="MESCA003680"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152081_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003680-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12821" checksum="D67B5077EE788710" modified="2013-10-16" version="1">MLYSSECWALTSKDEIMILTFESFVGREYEVLEQLISGPLVALISGDAMKRFHHRLDVIQSSDCESYKTDHAIQIVGYDMTAKIPYYIVRNSWGTGFDWLIFLRKQAGMGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJR2</accession>
    <name>T1GJR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zasp-like motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJR2"/>
    <dbReference type="STRING" id="36166.T1GJR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003712-RA">
        <property type="protein sequence ID" value="MESCA003712-PA"/>
        <property type="gene ID" value="MESCA003712"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2856316_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zasp-like motif" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003712-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7149" checksum="A23D858304182895" modified="2013-10-16" version="1">MQYVYDAMRSYSIQSHSIQLYHVNGNGPVKSIVNKQYNSPVGIYSDEAIAETLSAQAEVLAEGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJV5</accession>
    <name>T1GJV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJV5"/>
    <dbReference type="STRING" id="36166.T1GJV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003760-RA">
        <property type="protein sequence ID" value="MESCA003760-PA"/>
        <property type="gene ID" value="MESCA003760"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1588351_0_0_1"/>
    <dbReference type="OMA" id="EYSIRFD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1020">
        <property type="entry name" value="Peptide N glycanase, PAW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038680">
        <property type="entry name" value="PAW_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006588">
        <property type="entry name" value="Peptide_N_glycanase_PAW_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04721">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00613">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51398">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAW" evidence="2">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003760-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19576" checksum="FE8B8871DCB0C77A" modified="2013-10-16" version="1">MSFQALDEEIDKKQFNLRYHCSSDKYERYIKESNGSINIISTYDTWEACQFSSVNIFRKVEKDWKMAYLARNENSNFAEITWKFDFGSSNLVIKEYSIRFDKQTYENGNVQLEIVPDNKSLNVKGSSAFTIKANLSGGKGDCAWQHSQLFRQPLSSKDFPKGNFFFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJW3</accession>
    <name>T1GJW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003770-RA">
        <property type="protein sequence ID" value="MESCA003770-PA"/>
        <property type="gene ID" value="MESCA003770"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2335996_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003770-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11150" checksum="E686C11DA46FA7D4" modified="2013-10-16" version="1">MLTYVITFLGEKTYDRIEAQRSMNNGTTCSNVPSKGYKKEDMDIEYLILRSKDGLSSWGTTKSWLTYPIGKGQLKIEVLVNQAESALSGRTFKLQYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK33</accession>
    <name>T1GK33_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK33"/>
    <dbReference type="STRING" id="36166.T1GK33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003847-RA">
        <property type="protein sequence ID" value="MESCA003847-PA"/>
        <property type="gene ID" value="MESCA003847"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1373644_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000129">
        <property type="entry name" value="CLUMA_CG018772, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF36">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 9, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="75"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="23304" checksum="855ED2439888CD77" modified="2013-10-16" version="1">MTFHTKRKPNSNIDECYVFPSYFGPKEFKNEVKKILSPLSPENPYKITSFLCFFTANHQFNFEHYDNDEDIFTTPNLSHDKLLRNSVYFDKRYIQNVTTIAGQTAFLNCRVRNIGKKTVSWVRHRDINLLSVGKDIYSSDERFVALHNKETGDWTLQIKYANRKDTGAYECQVSTTPPIGTVLYLQVVDFLTQKPQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK45</accession>
    <name>T1GK45_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK45"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003860-RA">
        <property type="protein sequence ID" value="MESCA003860-PA"/>
        <property type="gene ID" value="MESCA003860"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471643_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003860-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10498" checksum="8FDE3B075C64B379" modified="2013-10-16" version="1">MDIIFCRICAARADIFHKIAHEKYNLYVTCSHKVAEQQRECPNKNNTEILNDLLAESNWIDELFRNIRNSWAEVESLERQKRFLEVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKD7</accession>
    <name>T1GKD7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CAQQ02199643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKD7"/>
    <dbReference type="STRING" id="36166.T1GKD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003960-RA">
        <property type="protein sequence ID" value="MESCA003960-PA"/>
        <property type="gene ID" value="MESCA003960"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1039333_0_0_1"/>
    <dbReference type="OMA" id="MEMNSTH"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003960-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="268" mass="30855" checksum="BAA75A84DDB5E46E" modified="2013-10-16" version="1">MSRTLPNILVLPIVLLGLSAWLNRKLKQFIWYSGIGVLIFRFELVIFFGLLLLHDIYFKAITLKSLFKNIFLAGVSILVLTISLDSFFWNRLLWPEGEVLWYNTILNKSSNWGTSPFLWYFYSAIPRATGFTAFFVPFGLYMERRVRVLGLCSLLFVFLYSFLPQKNLASFCSKVWMNMKKSNFSKILALCCAGHLFINILLTIFLLVVSSKNYPGGYAISRLHSAENASLKSVHISNLVAQTGFTRFFQHKDWTYNKTENLKYRGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKI4</accession>
    <name>T1GKI4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02048786">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKI4"/>
    <dbReference type="STRING" id="36166.T1GKI4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004009-RA">
        <property type="protein sequence ID" value="MESCA004009-PA"/>
        <property type="gene ID" value="MESCA004009"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_10_3_1"/>
    <dbReference type="OMA" id="HKCKENG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000135">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004009-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="11172" checksum="2743F745181579D0" modified="2013-10-16" version="1">MKFVTEIWHPNIEKNGDVCISILHEPGDDKWGYEKASERWLPVHTVETILISVISMLADPNDESPANVDAAKEWREAYPDFKRKVARCVRKSQEDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKJ1</accession>
    <name>T1GKJ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKJ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004017-RA">
        <property type="protein sequence ID" value="MESCA004017-PA"/>
        <property type="gene ID" value="MESCA004017"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3144422_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004017-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="49" mass="5686" checksum="AEA8FB658A2C01B3" modified="2013-10-16" version="1">MLNYKMSELIRPPPTPVNYRILKKMKADGEIKGHERCQGECIPLREDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKK6</accession>
    <name>T1GKK6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02044732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKK6"/>
    <dbReference type="STRING" id="36166.T1GKK6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004032-RA">
        <property type="protein sequence ID" value="MESCA004032-PA"/>
        <property type="gene ID" value="MESCA004032"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_10_5_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF506">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE PMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004032-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23180" checksum="075FF576D49AA481" modified="2013-10-16" version="1">MGIANPLNAMQILWINIIMDGPPAQSLGVEPVDQDVLKQKPRNVKQPMITRALIVNVLLSASIIILGTLWVFQREMSDDGIGGLSGINGGQRTKRYTTMTFTCFVFFDMFNALSCRSQTKSAFKIGLTSNRMFLMAVAFSIIGQMLVIYFPPLQMVFQTEALSAYDILFLISLTSSVFIVSEIKKWFERSMERKMHKTRSELDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL54</accession>
    <name>T1GL54_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02076997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL54"/>
    <dbReference type="STRING" id="36166.T1GL54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004244-RA">
        <property type="protein sequence ID" value="MESCA004244-PA"/>
        <property type="gene ID" value="MESCA004244"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083417_0_0_1"/>
    <dbReference type="OMA" id="CENRRDL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21407:SF5">
        <property type="entry name" value="HL04814P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21407">
        <property type="entry name" value="RE43931P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="36"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004244-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="25198" checksum="2A29E67489A4641A" modified="2013-10-16" version="1">MLIQIENIEFGSVFSIILFKSPLTQIDFLGPKEFSYRGRNMFLSSHVPALKDKKFDWLDGRNLCREYCMDLVGLETQEKNNLIFRVIQQNDVPYIWTSGRICDFNGCENRPDLEPKNIFGWFWSATREKIQATNRIPIGWGYNPWSQTGHKKRPQPDNAEYDINQTKEQCLSVLNNVYNDGIAWHDVACYHEKPVICEDNDELLRYIAATNPGIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL96</accession>
    <name>T1GL96_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390957">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL96"/>
    <dbReference type="STRING" id="36166.T1GL96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004294-RA">
        <property type="protein sequence ID" value="MESCA004294-PA"/>
        <property type="gene ID" value="MESCA004294"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_1"/>
    <dbReference type="OMA" id="ECDIANE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004294-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="251" mass="27434" checksum="042C4D91DA523CC6" modified="2013-10-16" version="1">MSSMERWNNKVALITGASSGIGATCAIDLVNRGMIVVAVARRKEKLEDLKNKVADDLKSRIIPIKCDVTVESEVVNCFNWVLENIGVVSVLVNSAGIAYAVDLVGKYTTNQLKTMVDVGIIGTALCVRETFKQMKEHSIDGHVILMNSTVGHNVPPFPLCSMNIYPSVKYATTAMAETYRREFSNAGTNIKVTSLCPGEVNSGIWPPELRDLVDITMLHPEDISNAILYCLSTPPNVVISELFIKPLHGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLV0</accession>
    <name>T1GLV0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLV0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004514-RA">
        <property type="protein sequence ID" value="MESCA004514-PA"/>
        <property type="gene ID" value="MESCA004514"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888138_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004514-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="15182" checksum="A53BB0FC796BAF35" modified="2013-10-16" version="1">MAVSNPYIGGRLMMARNLAVDVGQEWIVLKCAWTIHLTRLGASNSVMLMFYGNEISNILAKQVIDCHISWAPKVSPPVSYGKEYLFSRMNENCKKRWLSIDCVVAFSTSTHCIFFTDREESSWDNSDSGMSSCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM09</accession>
    <name>T1GM09_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02151202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM09"/>
    <dbReference type="STRING" id="36166.T1GM09"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004577-RA">
        <property type="protein sequence ID" value="MESCA004577-PA"/>
        <property type="gene ID" value="MESCA004577"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_610143_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08317">
        <property type="entry name" value="Death_ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="46"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004577-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="449" mass="51931" checksum="8900492501156812" modified="2013-10-16" version="1">MDNSERQEIISRLGLTSMCISRSYEPYHKPNIPRQSSTEDTQHFIGDMRIVDVSNLLGDDWMCLAPEIGITPTEVSNIKAQNENSVAQQAQAMIQLFKVKKNNDQNILENGLKNIGREDIIRKCVTVRKRIHLDAPHETDNMKMSESVEELMRREDISRLQQLHEREEFKYSAEEKEVEESESDEEMHKRTVAEDDKKSKNDFHQEITEIKRKSLIEDKKAIHEEEILMQMPTDNSHKSSVVPEQVIKLKHSKLDGGDAVDKGEFDKELQDKFKKTVRLSDEEEDFLVEENEPASKIDIDIQDSEKQKRSQIPVASKRSSQIFSEDGIKVKVIETEDPDLEDKLSKQKAKIEQEIDDIVPKTYTEKPRESLTEEQFIKSVEDTITKKMSEGRIEISDADIAKNAARLIMLASSILLIKIVLEIPARDEALNSFRCFDWINLRKRGYFCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMJ0</accession>
    <name>T1GMJ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMJ0"/>
    <dbReference type="STRING" id="36166.T1GMJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004765-RA">
        <property type="protein sequence ID" value="MESCA004765-PA"/>
        <property type="gene ID" value="MESCA004765"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3299936_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004765-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="40" mass="4777" checksum="98879489E69E7E20" modified="2013-10-16" version="1">MSASTEEDKLEWIQRLKQSISHNPFYDILIQRKNKASTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNA8</accession>
    <name>T1GNA8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02065764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005052-RA">
        <property type="protein sequence ID" value="MESCA005052-PA"/>
        <property type="gene ID" value="MESCA005052"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1148347_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="25279" checksum="85855A2DA3C6115A" modified="2013-10-16" version="1">MDSSPDLTLKLRRGSSDSRDSFYMDFAQGIDSDIEEVESSVLVNNSNVTAGVVVAPPPPPPPLDDTGSVTTCVKATMIHRKRKNSTKEEPEDPSTSPSPPPTPPPCPPLSVAPPPSPPHHMTPMTPPDSPSHSLSGHQLSSLVLHQFEHSSDMANNDDDSTTITTPPSNGCSTLTPPVAAQLGSNAADDTIISQPSYGNISKSLNEKSTSIASATLSGLTGTGTGTHTENTSNSLLKNDSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNV5</accession>
    <name>T1GNV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tektin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02145466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNV5"/>
    <dbReference type="STRING" id="36166.T1GNV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005266-RA">
        <property type="protein sequence ID" value="MESCA005266-PA"/>
        <property type="gene ID" value="MESCA005266"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033588_2_0_1"/>
    <dbReference type="OMA" id="CMEPISG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF11">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="516"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005266-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="573" mass="66348" checksum="7F6DF5BF270AF949" modified="2013-10-16" version="1">MLCWRNEDNYLRQTYTDPYRGENRALKKLLQKSSIPISHGSSEFTMKWHTNCYLENRAFDSRLVKEDLVNWSSSEPLGQYPERKDFRNLMPSYPWSTANGDPCKQAMFGIPPRQGTSYMNPRALPWKPSNELIEVNPTLPERLAHQEFSSIGAQSESITFPNLVTGFQRNPQHAARAALYTRYTPGEWVENCLSKFSESNVNRNISERLRNEAVRVIRETDEKICQGQRDAGRRLGERLTDVTFWRNELNTEMEKFSLKYQAKKKTGKALEDLEGPLHVAQECLYNREKRKDMEKVHDNVEKSLLLEIDVIRRSQAQLRDIYEKASKQLQDSRSAQFALEEDVGLKESALGIDSVCHQLNNNSRGINYYGGIEKFDPTISTMEAWAQSSASRINKSQHERGKSVQIRSEIDTAVNATTSRVWDHWSNTNNALDRRSSELSEAKNKVQLHLHKVQNEIFDMERHIALLQKAIHDSSHPLKVAQTRLEARAHRPGVELCKDFAHMRIVQEVHEIHDGVRSLHHKLQEAESQHQQLLKTRASLEADLHKKVESLFIDREKCMGLRRSFPVDNMIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPC0</accession>
    <name>T1GPC0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005445-RA">
        <property type="protein sequence ID" value="MESCA005445-PA"/>
        <property type="gene ID" value="MESCA005445"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830199_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16670" checksum="81F27CED3AF2CC7E" modified="2013-10-16" version="1">VFVYINIFGNTVDICYCFCYHLLLLVLISTNKFQFHYAPAFHRMHSEYLLILPQKYTPFYTKINNELKNNPLEVFRNGETIKKVAKHIVKRPYPVLPLVCSLDNKDFQELCIQQAWGLMNSDWDLEKLLIFVVFTGELGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ99</accession>
    <name>T1GQ99_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ99"/>
    <dbReference type="STRING" id="36166.T1GQ99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005803-RA">
        <property type="protein sequence ID" value="MESCA005803-PA"/>
        <property type="gene ID" value="MESCA005803"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278939_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="158"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005803-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="23289" checksum="5B90E3758A4871EA" modified="2013-10-16" version="1">MQSYTDHMAAVKAIAWSPHHHGLLASGGGTADRCIRFWNTLTGQPMQSVDTGSQVCNLAWSKHSSELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLALSPDGEAIVTGADPVEACKATTKGIITPGGTHSGVNPFALAALRARILEAPTTMSKEMSFSEEDRLLGGDPMASLLNKENYRAEEQRNRSQDEDIQPPGKRIPAATRGGPSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQG9</accession>
    <name>T1GQG9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02169011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQG9"/>
    <dbReference type="STRING" id="36166.T1GQG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005876-RA">
        <property type="protein sequence ID" value="MESCA005876-PA"/>
        <property type="gene ID" value="MESCA005876"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_858653_0_0_1"/>
    <dbReference type="OMA" id="ERTHRCK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055059">
        <property type="term" value="P:asymmetric neuroblast division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060562">
        <property type="term" value="P:epithelial tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000177">
        <property type="term" value="P:regulation of neural precursor cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000450">
        <property type="entry name" value="PR domain zinc finger protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000043">
        <property type="entry name" value="Scratch family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000706">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000207">
        <property type="entry name" value="zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF38">
        <property type="entry name" value="PROTEIN SNAIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005876-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="324" mass="36508" checksum="1365DC66F355C5CB" modified="2013-10-16" version="1">MQQQNQQTSRCNKSTPNPVMQEISNEQDANGNNNSSSMVVVSGRSGGGYQRTYTHRSIQTKSRYSYENNNTENCSPLTPPNSEYSSDIDLDLLSVSDATLMTPEELWNSEMEAFKSGKTTLIVDGRSRSRKNYDPSKPRRPAKKGMYRCAECGKNYATSSNLSRHKQTHRSLDSQSAKKCNICSKVYVSMPALAMHLLTHKLSHSCQWCGKLFSRPWLLQGHLRSHTGEKPYICVYCNKAFADRSNLRAHTQTHSGERTHRCKRCNKTFALKSYLNKHLESTCSGIKMEVMEDMDCDDMDGDMDDEFTDDVDGEGDGDGEVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQM3</accession>
    <name>T1GQM3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02043376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02043377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQM3"/>
    <dbReference type="STRING" id="36166.T1GQM3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005934-RA">
        <property type="protein sequence ID" value="MESCA005934-PA"/>
        <property type="gene ID" value="MESCA005934"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_3_1"/>
    <dbReference type="OMA" id="YKQDGVG"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00202"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005934-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="331" mass="36460" checksum="B6F384698F8978B5" modified="2013-10-16" version="1">MTLIDTFSTGFEYGRSGNPTRNVLEKCLASLDNGKYGLAFASGLGTTTAILTMMNSGDHMVIGDDVYGGTNRVIRQVAARLGITFDFVDPTDLKALEAAIKPNTKLFWVETPTNPLLKVVDIESVAKIAHKRDDIIVVVDNTFLTSYFQRPLELGADVVAYSLTKYMNGHSDVIMGAAVTSSEDIYNRLNLKTLALRMEQHQKNAIQIAEYLEKHPLVEKVLHPGLPSHPQHQLALKQTYGYSGVFSFYIKGNLETSRKFLKALEMFTLAESLGGYESLAELPVIMTHASVPAEDRVKLGITDTLIRLSVGLEDADDLIEDIEKAFKASQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRC7</accession>
    <name>T1GRC7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRC7"/>
    <dbReference type="STRING" id="36166.T1GRC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006207-RA">
        <property type="protein sequence ID" value="MESCA006207-PA"/>
        <property type="gene ID" value="MESCA006207"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2694994_0_0_1"/>
    <dbReference type="OMA" id="WFIVSNI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF30">
        <property type="entry name" value="SIDESTEP VIII, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006207-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10475" checksum="F93C2E8B47178356" modified="2013-10-16" version="1">MNRFFLTIHLSWNFDRRDLSTDTGSHWADDSLGRRAYFHVAARPAALTIKNTTAEDNGVYRCRVDFQKSPTRNSKVELRVISLWFIVSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSD8</accession>
    <name>T1GSD8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSD8"/>
    <dbReference type="STRING" id="36166.T1GSD8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006593-RA">
        <property type="protein sequence ID" value="MESCA006593-PA"/>
        <property type="gene ID" value="MESCA006593"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161097_0_0_1"/>
    <dbReference type="OMA" id="YFTELVY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006581">
        <property type="term" value="P:acetylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577591" description="Carboxylesterase type B domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="22"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006593-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12342" checksum="099C8AF572278126" modified="2013-10-16" version="1">MLKILIAVSFFYFHSVSSHELLVKAPIGQMRGVIMESQKGYKIYAFKGIPYAEPPVGPLRFRDPVPKSKWCGVLDATDDGYTCPQYEMLFKNMNQSEDCLVLNVYKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSY0</accession>
    <name>T1GSY0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02114471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSY0"/>
    <dbReference type="STRING" id="36166.T1GSY0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006794-RA">
        <property type="protein sequence ID" value="MESCA006794-PA"/>
        <property type="gene ID" value="MESCA006794"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046597_2_0_1"/>
    <dbReference type="OMA" id="FPHMSTQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF120">
        <property type="entry name" value="CELLULAR RETINALDEHYDE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="104"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006794-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="327" mass="37955" checksum="78ACE695E7EB1AB5" modified="2013-10-16" version="1">MGYPWKSEKIKPAVDENCNVSENLMKIAKKELREDKHTREQALQQLRNWINKNPDIENLRSDDQFLLRFLRVKKFSVPMAEQVLLKYLNLRKTYPHMSMNLDFLEPRVNSFMSNGYIFASPIRDRHGRRVVIMSAKGINPKIHTSTDQAKSHFITYETLMEDTETQILGLTHIADFSGVTTAHVTNWNPTEFARVFKWGEQSFPLRHKEIYLVNVPSSLKWVVDFVKNRVSAKIKNRFHCYTNVKDLHKHVDPDCLPQELGGKILMADMVESWKKELIAKRDLVINLDKMNLLSDRGITTSKNNNISTNSDGGIMTQWNPLPEVSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT38</accession>
    <name>T1GT38_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Regulator of MON1-CCZ1 complex N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02009735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT38"/>
    <dbReference type="STRING" id="36166.T1GT38"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006858-RA">
        <property type="protein sequence ID" value="MESCA006858-PA"/>
        <property type="gene ID" value="MESCA006858"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275059_0_0_1"/>
    <dbReference type="OMA" id="QDIRIFT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040371">
        <property type="entry name" value="RMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049040">
        <property type="entry name" value="RMC1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897">
        <property type="entry name" value="COLON CANCER-ASSOCIATED PROTEIN MIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897:SF4">
        <property type="entry name" value="REGULATOR OF MON1-CCZ1 COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21029">
        <property type="entry name" value="RMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50960">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Regulator of MON1-CCZ1 complex N-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006858-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24066" checksum="478A170C8B94EF03" modified="2013-10-16" version="1">MDSFYLELSSSPIKFDSVSSLTNVFFDESNKQIFAVRSGGTTGVVVKGPTNDSVISFCMDNTGIIRSIKFSPDNEILADPQQPNRNRGDIFKYTTKSSTIHGFVWVSKRECAVISNSSVELFTINPEKKQIKSLKSLSIGIKWFAWYSEGNMALISTTDVNALYPVLVNQKVITKLPKLELGSNTAEIPERDVTLGQLYGSLAILLLQQTSNRLLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTW2</accession>
    <name>T1GTW2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02134300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTW2"/>
    <dbReference type="STRING" id="36166.T1GTW2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007156-RA">
        <property type="protein sequence ID" value="MESCA007156-PA"/>
        <property type="gene ID" value="MESCA007156"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159020_0_0_1"/>
    <dbReference type="OMA" id="AENRQGY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059031">
        <property type="entry name" value="SH3_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF7">
        <property type="entry name" value="STAC-LIKE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26085">
        <property type="entry name" value="SH3_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007156-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10761" checksum="F0DAD71A1506ECA0" modified="2013-10-16" version="1">VTVIDTSDPDWWKGKCLGRVGFFPSKYCARLNAGEKPLQVTHNLQVSDSDRGENMTLLRDQIVIQTEEEINGMVRVRSAENRQGYCPMKYLQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTX6</accession>
    <name>T1GTX6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTX6"/>
    <dbReference type="STRING" id="36166.T1GTX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007174-RA">
        <property type="protein sequence ID" value="MESCA007174-PA"/>
        <property type="gene ID" value="MESCA007174"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_2_4_1"/>
    <dbReference type="OMA" id="EACIAWV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007174-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="106" mass="11516" checksum="DF972D89F74CFBB8" modified="2013-10-16" version="1">MDSFCPLGPSIVHKCYVKDPQNLTLTSKVNGVIKQNGNTKDMIYKIDEIIEHLTQAITLKPGDIILTGTPAGVGMHRSPPEYLKPGDVVESEIQCIGTLLNKVVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUH7</accession>
    <name>T1GUH7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02124713">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02124714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007390-RA">
        <property type="protein sequence ID" value="MESCA007390-PA"/>
        <property type="gene ID" value="MESCA007390"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1708741_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF154">
        <property type="entry name" value="IRREGULAR CHIASM C-ROUGHEST PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="70"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007390-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="16868" checksum="2C0A32AFAC441134" modified="2013-10-16" version="1">FTGDQELTPIGPQVNATEPDNSRTWSASSVVQVPAVRERHGDLLRCVAYHESYSAKSVSVEAKMDIKYAPTIRLIGAPSVDLEDENDSLTLRCISDANPPASIVWRRAGRSEIASLQETLILRPVSRRDAGLYTCQAQNTVGTSEQLSIQLDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWP9</accession>
    <name>T1GWP9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Capon-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02054631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054632">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWP9"/>
    <dbReference type="STRING" id="36166.T1GWP9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008227-RA">
        <property type="protein sequence ID" value="MESCA008227-PA"/>
        <property type="gene ID" value="MESCA008227"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014963_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008227-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="47715" checksum="DE153B54F51E8888" modified="2013-10-16" version="1">QLAITPDISIQAPPQRPNHLELGTHLNRGGNINNNTNHKSSMVKRWGKVSDITDTIIQNEVQKLKEQLEQQALQTRQALAQLIMVREQLISETNARMEAQARTQQLLQQNRELLEHLASLGAYTEPQQQSLTAANIAMAPQSHLSMLLDNQAKNMMSNNNLASINQQINNLGSINQQLTNLSHQLNGINQQQQGFQNYSAQNQTPNNNGNQPTMNQLQNISNQLSSISVQNNQEALNKELYQMNQDLLNRLQSMNLGMNHLTAATPNNNSLFFANPMSCSPNTNTNMNFLSTPSSYGNLTPSPVGTLNRNSFSQSHSPLPMINSTDGLGLSIDKNLNNLEDNAHIMKSRLGMMGGGVVNQSNNNISDSTRSDSSSSPLKLSDQRLNNIANNGNTVMLKVTDEAGNVTNTRKLSATPSFIQRSTAKKCQIVVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWQ1</accession>
    <name>T1GWQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02026251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GWQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008229-RA">
        <property type="protein sequence ID" value="MESCA008229-PA"/>
        <property type="gene ID" value="MESCA008229"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1300951_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008229-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24270" checksum="7B7EC2A94C5718EA" modified="2013-10-16" version="1">MRAAEIDTKNIITNKPTQILGYADDIDVVGRTTSSVQYIKASVFQRLEKEAKSKGLRVNADKTKCMPSSRNQSHANRCLFSLIRLIRSKKLSCKIKVRLYHQLILTVILVQPRTNPDIVKKIRTRRLRFAGHVERMDDNAAVYNDNVEGVRRRNRQRARWVDLVDKEGTKNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWV5</accession>
    <name>T1GWV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWV5"/>
    <dbReference type="STRING" id="36166.T1GWV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008287-RA">
        <property type="protein sequence ID" value="MESCA008287-PA"/>
        <property type="gene ID" value="MESCA008287"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1246615_0_0_1"/>
    <dbReference type="OMA" id="YSIITCK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF35">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577793" description="Major facilitator superfamily associated domain-containing protein" evidence="7">
        <location>
            <begin position="17"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008287-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="25044" checksum="FE53492397783145" modified="2013-10-16" version="1">MYMLLLFFSLPIAVFTFDWGTSSLFWKMKRVFKRDELLRPPRDPSIEQELEGRVSFRSIIDMPYSIKVLCLTGLLSWMSHCSYSLYFTDFVGETVFKGSPLGSDEQVELYDAGVRYGCLGLSVFSITVSLYSIITCKLVKLCGLKMMYSGTLLFYAFAMFLLAFEPNKFAVLAYSVPAGAAYATMLTIPFTLVASYHEKGCVTTFKKNQGQIISPARPSVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX56</accession>
    <name>T1GX56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02148821">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX56"/>
    <dbReference type="STRING" id="36166.T1GX56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008398-RA">
        <property type="protein sequence ID" value="MESCA008398-PA"/>
        <property type="gene ID" value="MESCA008398"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017375_5_1_1"/>
    <dbReference type="OMA" id="HEEMIGD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF122">
        <property type="entry name" value="ARGININE METHYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008398-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="24908" checksum="FBE5C154D495126C" modified="2013-10-16" version="1">MNPPGVDEQYFLDYQDLEIHELMLKDRPRQEAYFNAILKNKDLFKDKVVMDVGAGTGILSAFCAKAGAKTVYAVEASNLAKLALNIIQENNLSEVVKVIYSRVEDFSLPDNEKVDIIVSEWMGFYLLHEGMLDSVIHARNKFLKDGGLMFPTSATLYVAPCSVPSRYDYWDSVDGIKMNTFGEMLRSIKSHKPEITQIDADCLLHEGTVVYLYDFPNVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX77</accession>
    <name>T1GX77_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02078847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX77"/>
    <dbReference type="STRING" id="36166.T1GX77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008419-RA">
        <property type="protein sequence ID" value="MESCA008419-PA"/>
        <property type="gene ID" value="MESCA008419"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3338055_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008419-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="38" mass="4148" checksum="C2910F755833A2B2" modified="2013-10-16" version="1">MNSFSSPLLAVIAKNILFMTGGVLILILALGIYDEYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXN5</accession>
    <name>T1GXN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02166128">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02166129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXN5"/>
    <dbReference type="STRING" id="36166.T1GXN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008588-RA">
        <property type="protein sequence ID" value="MESCA008588-PA"/>
        <property type="gene ID" value="MESCA008588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_895949_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00199">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.22.10">
        <property type="entry name" value="Antistasin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004094">
        <property type="entry name" value="Antistasin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02822">
        <property type="entry name" value="Antistasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00003">
        <property type="entry name" value="4DISULPHCORE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51252">
        <property type="entry name" value="ANTISTASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Antistasin-like" evidence="2">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="3">
        <location>
            <begin position="216"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34661" checksum="69944E69C0FD8650" modified="2013-10-16" version="1">MCEYLRDFSERMEGTEAGMQLAITPPKCNEDGSYQARQCFLKKIKVTRAEQRKILEENTIRKMRALLTRPKRDVESLKLYRVDDSALNIQVAAPSMGRNAKVIDMGANRQQGLGELFPFETKKSASANKKKNDNELSRGFNVTTESCINLKDSLDCLDLTCRMGCEYGFTLDSDTKCPACQCRDPCDGVTCGEGTECRVVDVSCEDEYCPPVPACLPRKPGQCPFLVPPGSDNESNTCDYECRTDFHCDGSKRCCSNGCGTQCVEPQLKTACQHLQTIQLHQSSEMGIPARQMYIAQCDEVSGKWKEVQCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXU2</accession>
    <name>T1GXU2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXU2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008649-RA">
        <property type="protein sequence ID" value="MESCA008649-PA"/>
        <property type="gene ID" value="MESCA008649"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136299_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588591" evidence="1">
        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008649-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="13183" checksum="A7A84CD8E126B5F7" modified="2013-10-16" version="1">MSMKSIIILLLFLTRKLQGNDNLENCIRECEEGNVPVLDCRNLPDECRNFKDFENQMEDCKEICEKKVLETEMGNIRKVRITNLSYKMQVASCIFVPISLVSCKIMYILIRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY85</accession>
    <name>T1GY85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Attacin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the attacin/sarcotoxin-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02101644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101646">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008807-RA">
        <property type="protein sequence ID" value="MESCA008807-PA"/>
        <property type="gene ID" value="MESCA008807"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1410303_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005521">
        <property type="entry name" value="Attacin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03769">
        <property type="entry name" value="Attacin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588599" description="Attacin C-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Attacin C-terminal" evidence="9">
        <location>
            <begin position="96"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008807-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="193" mass="20275" checksum="1F3741B200B49219" modified="2013-10-16" version="1">MLLMKLWLSLSCAFVAVYGDSISEGSQLKADVNTKIGELNVAGVAKGNNHGQSNIEIGADKNGNSLKVNYHHAPGSNSYGSTGTINLHKADTSKIDNANGHEAFVGVNHSPQAHSTNIFGGGKANLFTSNDKSTNVGLTAGVQQSRSPLGVHTEKNVGIQLQIKLTSPPAAAPVNYFEGCCKCCWGQKDSCNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYA6</accession>
    <name>T1GYA6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYA6"/>
    <dbReference type="STRING" id="36166.T1GYA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008829-RA">
        <property type="protein sequence ID" value="MESCA008829-PA"/>
        <property type="gene ID" value="MESCA008829"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021225_0_0_1"/>
    <dbReference type="OMA" id="PKSDYYR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010301">
        <property type="entry name" value="RRP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026">
        <property type="entry name" value="NNP-1 PROTEIN NOVEL NUCLEAR PROTEIN 1 NOP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05997">
        <property type="entry name" value="Nop52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="404"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="465"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="317"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008829-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="515" mass="59491" checksum="8226233C637C35EE" modified="2013-10-16" version="1">MISKVESEENVKFQEDDFARIWKGLYFCMWMSDKPLVQEELAEDLGKLVECFSTKVGIDFFGSFLDIMQQEWFGIDQWRMDKFLMLVRRMLRYTLKAIKKEKWQSDFIEKLNSVVYKCLESPCLGLSMHVLDIFFEELAKMGFPTKSIDDIELVKPGVVDDSGKNKNQHLDPRAGNVDVFMPILPLDADGIIQEIERVMYKSFTNSKSRKHLKIILENFKTYKSGVFPLGVKTMPILEKGIMKPIVEEELRKIEELDKEMHNSRRSLKSLSKKQRKRFLASLDLSEANENNIDEIIEKQLNKSPYAKKKKFQQKLNSLDKEVPAKKQKLEQKVSTESSEKNKEETPSKKPILKQEENSETIKKNKLKNKKEPFKADSEWDVPMKEGETEYFVPSKKIQIKKANEALKNDDLVPNPFAKLKDSVDSPKTPKQKLKGKSLKLSTPLSTPGKKKVRIVLKQNASQSVAEHIKQVRSSPLNPYDATKLPTKGLLKPNSMPGPINPYYKKKLKLSFNDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYK5</accession>
    <name>T1GYK5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02388137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYK5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008937-RA">
        <property type="protein sequence ID" value="MESCA008937-PA"/>
        <property type="gene ID" value="MESCA008937"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2339504_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008937-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="10582" checksum="18EB4C3731FC0B8A" modified="2013-10-16" version="1">SNGTESTSIDKCKESLKELSTVNSLRLEGHEEIDGNEIANDLARKGSVTPGGESLNLLGRSVKPPMKPSYSVCPAVSSRPLGSRWSKEGWIRVTVLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU5</accession>
    <name>T1GYU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYU5"/>
    <dbReference type="STRING" id="36166.T1GYU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009032-RA">
        <property type="protein sequence ID" value="MESCA009032-PA"/>
        <property type="gene ID" value="MESCA009032"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507099_0_0_1"/>
    <dbReference type="OMA" id="ICTIACL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000144">
        <property type="entry name" value="Picot, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF280">
        <property type="entry name" value="FI21844P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009032-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="19672" checksum="2B394445C7D24F8D" modified="2013-10-16" version="1">FDWTNGQVQYVLSSFYWGYCLTQFFGGWLCKRYGVKITLFSAITICTIACLVTPVTAKAWSWQGYCALRVIQGLAQGLIFPCMHEHVARWAPKQEKNRLGAFANSGLELGTVVALGTSGVIASSDMGWPGISYVAGGLGVIWAVMWLLFASNSPSDSKKISEVERKYILASQDHDSSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ00</accession>
    <name>T1GZ00_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02188615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ00"/>
    <dbReference type="STRING" id="36166.T1GZ00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009088-RA">
        <property type="protein sequence ID" value="MESCA009088-PA"/>
        <property type="gene ID" value="MESCA009088"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471650_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF84">
        <property type="entry name" value="AGAP003685-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="15"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009088-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9909" checksum="CDFD7BADDDCFE2FE" modified="2013-10-16" version="1">MCRGFHSLQKDEKMVDVTIAAGGKIFKAHKLVLSVCSPYFQQIFLENPSSHPILLMADVESNHMAGLLDFMYSGQVSQISRTFLYVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ08</accession>
    <name>T1GZ08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ08"/>
    <dbReference type="STRING" id="36166.T1GZ08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009098-RA">
        <property type="protein sequence ID" value="MESCA009098-PA"/>
        <property type="gene ID" value="MESCA009098"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944331_0_0_1"/>
    <dbReference type="OMA" id="VENNMYC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051184">
        <property type="entry name" value="Tyrosine-phos_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969:SF14">
        <property type="entry name" value="DREADLOCKS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969">
        <property type="entry name" value="SH2-SH3 ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009098-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="7149" checksum="F43D0415924E2459" modified="2013-10-16" version="1">MYMGDFSVSLKAPGRNKHFRVHVENNMYCIGQRKFHTLDQLVDHYQRAPIYTNKQAHENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZA6</accession>
    <name>T1GZA6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZA6"/>
    <dbReference type="STRING" id="36166.T1GZA6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009204-RA">
        <property type="protein sequence ID" value="MESCA009204-PA"/>
        <property type="gene ID" value="MESCA009204"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2111652_0_0_1"/>
    <dbReference type="OMA" id="MMSHENY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009204-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12377" checksum="33935171662E1BF5" modified="2013-10-16" version="1">MALRVQFENNGDIVNPYLDKETEEIIGDVLGVEATIQEQDELSSLLQVPLVAGTCNRGSEVLAAGMVVNDWISFVGMSTTATEISVIESVFKLTSNAQPSTVTTKLRAALIEDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZE9</accession>
    <name>T1GZE9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009248-RA">
        <property type="protein sequence ID" value="MESCA009248-PA"/>
        <property type="gene ID" value="MESCA009248"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123910_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009248-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="13089" checksum="1B1CD49D1D7B382D" modified="2013-10-16" version="1">MQRGLPSCLCEIPIKSDLLQRSAGCKNKILRFPKRTSKRIDDITTRDQMASKNLNGKRCQKNGASILLAVMYNNFPYIAIIQQIINRKLKLRQKRSKDFSNDSYKCIGNPKLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZL6</accession>
    <name>T1GZL6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZL6"/>
    <dbReference type="STRING" id="36166.T1GZL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009319-RA">
        <property type="protein sequence ID" value="MESCA009319-PA"/>
        <property type="gene ID" value="MESCA009319"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_853335_0_0_1"/>
    <dbReference type="OMA" id="NNCKENG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009319-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="326" mass="35659" checksum="3122AB56256DE655" modified="2013-10-16" version="1">MDINVSAAQNAGSGGKKNRQKERRLMKGFNIIAPPGSTGSLAPVQEFSTSLDQCEAQTEIINSTLSNLFSTSFLHKNKSTTQSCSTNNFNSFNSSSTNSSVSNKDGLPPLHEHIAGTKYNKNCLKTYNKRISGQKKKWGNVIEAAKLGNMSKILGRSKSEDSVYNSMNSPIVQQGIKVSYAQDTGQYSSDNNSTSSNGNANVNGGHGNNSISRRFKEKRKKFSSSKNISPVTESISMSPESDKVRDEQNRKMSSNIIISKALKRVSSYPACPSPETKEVAKPRRHEQTQSQHESVETTSEYTISSNELPLDPSSREPLLNAIIANH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H081</accession>
    <name>T1H081_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H081"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009553-RA">
        <property type="protein sequence ID" value="MESCA009553-PA"/>
        <property type="gene ID" value="MESCA009553"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1526922_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009553-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="176" mass="18901" checksum="0E97D25E81540A66" modified="2013-10-16" version="1">MCFSTNGLHMSSSSSVGVLLAGGRQNTTLLIYTFSRLRPILLSHSLSFSPLLPQNGMPVLSPFSPGASPIIMIFASVLPLEKTVLFAFSFILPPSNDATAFLSLPTIHILNRVEKVANNAIGRTSRHIAFEKATCDIGLQDSEVNHENDVSAVGKQDVNFKFCTGNPESPSQVGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0I1</accession>
    <name>T1H0I1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0I1"/>
    <dbReference type="STRING" id="36166.T1H0I1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009659-RA">
        <property type="protein sequence ID" value="MESCA009659-PA"/>
        <property type="gene ID" value="MESCA009659"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_1"/>
    <dbReference type="OMA" id="HIFIHAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="164" mass="17963" checksum="58684113DF1E6FEF" modified="2013-10-16" version="1">MPPGSKVLYLGAASGTTVSHVSDIVGPEGLVYAVEFSHRPGRELINVAKKRTNIIPIIEDARHPHKYRMLIGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHFVISIKASCIDSTAQPEAVFAAEVKKMQADKLKPQEQITLEPYERDHAVVVGVYRPLPKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0V9</accession>
    <name>T1H0V9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02069413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0V9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009804-RA">
        <property type="protein sequence ID" value="MESCA009804-PA"/>
        <property type="gene ID" value="MESCA009804"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940508_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009804-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="15337" checksum="524DEFB8CBADF924" modified="2013-10-16" version="1">MVNGDNTKYTLSSRRERSQPTRKPESHHGKVQHRGKSQLPLKWNTKVHSNTFRLRGPMIFMEPQTLIFFQENYSSQNPGFDFIVLKFMLPKARDRSFTAKTERITSKMSKILKVKTKIKLYFVLLNICFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H110</accession>
    <name>T1H110_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H110"/>
    <dbReference type="STRING" id="36166.T1H110"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009861-RA">
        <property type="protein sequence ID" value="MESCA009861-PA"/>
        <property type="gene ID" value="MESCA009861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_18_2_1"/>
    <dbReference type="OMA" id="QKEFYRV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000077">
        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="18078" checksum="B7064732A62D029F" modified="2013-10-16" version="1">MAPRKKSSVNAKDGESTEIREAFDLLDGEKTGFIEIDDLKSGLKELGLEQTKEDLNILMADLKKDKSGKISFLEFQKIVTDKIEENEMREEMLKAFTFFDKDKTGAISFKNLKRVAKELGEKISDEEILEMIDEADLDGDGEVNQKEFYRVMKKTNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H165</accession>
    <name>T1H165_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H165"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009919-RA">
        <property type="protein sequence ID" value="MESCA009919-PA"/>
        <property type="gene ID" value="MESCA009919"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3351619_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009919-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="37" mass="4407" checksum="6535D19FFD87554A" modified="2013-10-16" version="1">MMLTQNGKLLYISDNAAEYLGHSMFYQKRQKTRNINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1A5</accession>
    <name>T1H1A5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1A5"/>
    <dbReference type="STRING" id="36166.T1H1A5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009962-RA">
        <property type="protein sequence ID" value="MESCA009962-PA"/>
        <property type="gene ID" value="MESCA009962"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2475303_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009962-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9016" checksum="0D574BA3586E2482" modified="2013-10-16" version="1">FSGSANEPPANAFVSDNNFSTVFGNSNPTGFDVLGDVLKPTNDQFANPTNVFGLPQDPVADANAPPAQNTASSGKIITGDLDSSLMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2A3</accession>
    <name>T1H2A3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02158776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2A3"/>
    <dbReference type="STRING" id="36166.T1H2A3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010340-RA">
        <property type="protein sequence ID" value="MESCA010340-PA"/>
        <property type="gene ID" value="MESCA010340"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145019_0_0_1"/>
    <dbReference type="OMA" id="YCCETAD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000935">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052385">
        <property type="entry name" value="Obscurin/Obscurin-like_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971:SF5">
        <property type="entry name" value="OBSCURIN LIKE CYTOSKELETAL ADAPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971">
        <property type="entry name" value="SI:DKEY-31G6.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="31"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="126"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010340-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27561" checksum="B8BFF5B9A40B296B" modified="2013-10-16" version="1">MNPKVEDSGKYTVEIGGISSTAFLNVEEADPTYSFTKPLKKKYEGFTLHETTLECTVSNSLAIVHWFKDDKKIESNDERYLIGKNLDGQCKLVIRSGVLEDSGKYTCKLDKQPDKTESNVKIVEYPYKFVKVLKSLQCVEKDSITLACELDDDGGEVQWFKDDVEIKPDKRIQVVKEGRKRKLVIKDAKVTDAGQYKCTTNADKTEAELIVSYQNAFNKKLKDTVGIERNKLVLEVELQDKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2B6</accession>
    <name>T1H2B6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02372751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2B6"/>
    <dbReference type="STRING" id="36166.T1H2B6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010356-RA">
        <property type="protein sequence ID" value="MESCA010356-PA"/>
        <property type="gene ID" value="MESCA010356"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1338918_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577323" description="Farnesoic acid O-methyl transferase domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010356-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23706" checksum="0A6E688C17AB564B" modified="2013-10-16" version="1">MLKLLIIFHLFYAQSFCFDKGISFYSYRNCEREVIVEGDGSESGYMRFQNFIPIANLSYNKANNGEILSLRFYFQGRADFSVIFSPQPKQPSSTEKIFQTVLAYGKGGERRSSIFLNNVVDIYKTTKCDHVDNIPNPMDPFFFQQVDVIVNKNYGLIMWFIPQHGRSPLLTCFDPNIVNIKYIGFSGFSRNKVRYIFDCPIKNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2H3</accession>
    <name>T1H2H3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2H3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010419-RA">
        <property type="protein sequence ID" value="MESCA010419-PA"/>
        <property type="gene ID" value="MESCA010419"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725096_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010419-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8148" checksum="A4FC176D3656D9E7" modified="2013-10-16" version="1">MVTTMAAMERVLQNAPARKTARAAVVPDPQPGTRVLALDLTKKFLRILKYLMIITKNNNLIVFHTFTTYNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2K3</accession>
    <name>T1H2K3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2K3"/>
    <dbReference type="STRING" id="36166.T1H2K3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010452-RA">
        <property type="protein sequence ID" value="MESCA010452-PA"/>
        <property type="gene ID" value="MESCA010452"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691155_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="11"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="17858" checksum="9FF88BB419F59B7C" modified="2013-10-16" version="1">CGEVFSRTNRIVGGHATGFGSHPWQVALIKSGFLSRKLSCGGALISNRWVVTAAHCVATKMENNRINSFPFRDLERLPILFSGYLFFFLLIHWHYRSYSQHLSSPPHSTGFWFCLNKGSNLPPRCHKLQWRSLGPMIHYDYGFYLIARHLSSPIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2K9</accession>
    <name>T1H2K9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Collagen IV NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2K9"/>
    <dbReference type="STRING" id="36166.T1H2K9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010458-RA">
        <property type="protein sequence ID" value="MESCA010458-PA"/>
        <property type="gene ID" value="MESCA010458"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294846_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010458-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11256" checksum="5E76D5ED0DD3AEF9" modified="2013-10-16" version="1">MDHRLGDHKDHNHKDRKDHNLQYFHKDYMARKDHKVHKHNFSLDHKDYSPQGPHGPQGPQGPQGPPGSHGPQGPQGPHESPQGPQGEQGPHGPHNPQGRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Y9</accession>
    <name>T1H2Y9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02139679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Y9"/>
    <dbReference type="STRING" id="36166.T1H2Y9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010596-RA">
        <property type="protein sequence ID" value="MESCA010596-PA"/>
        <property type="gene ID" value="MESCA010596"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017286_2_3_1"/>
    <dbReference type="OMA" id="NTHNIYI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF814">
        <property type="entry name" value="MEPRIN A SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010596-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20013" checksum="1D138F8AD98083DE" modified="2013-10-16" version="1">MILTPDQRNILYNENGSKNGLINPALRWPKAVVFYKFATSIPQNHRSLIINSLKEIEKSTCIRFQQGVNSKNDYISVTTDAPGCFSNVGYLGKSQRLNLGYGCMIKRAIVHEFLHALGFFHQQSSADRDNFINIHWENIQKGTEYNFDKYSSAKITNFGTRYDYGSIMHYGPLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H372</accession>
    <name>T1H372_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02379043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H372"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010691-RA">
        <property type="protein sequence ID" value="MESCA010691-PA"/>
        <property type="gene ID" value="MESCA010691"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2415763_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010691-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="10411" checksum="C3952CF24342E1E4" modified="2013-10-16" version="1">MANKNGLPISNNYIFSSHDTRPDHLEITNDNLKISDIALALHQNFEDSQNFMSNYKQKINQTLKPPRGTIWKGNPLISAKFVGLQPQCGTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3E5</accession>
    <name>T1H3E5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-amylase</fullName>
            <ecNumber evidence="4">3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02126639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3E5"/>
    <dbReference type="STRING" id="36166.T1H3E5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010770-RA">
        <property type="protein sequence ID" value="MESCA010770-PA"/>
        <property type="gene ID" value="MESCA010770"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013336_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031319">
        <property type="entry name" value="A-amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43447">
        <property type="entry name" value="ALPHA-AMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00632">
        <property type="entry name" value="Aamy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-amylase C-terminal" evidence="9">
        <location>
            <begin position="222"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010770-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="311" mass="34725" checksum="40D2417A1674CA73" modified="2013-10-16" version="1">MWPIDCRNLYAKVKNLNTSFDFPENSRPFFYQEIIDMGGEAISKIEYVGYGVVTEFMASYILANALKGGQPLSDLLKWGTKMGFLHSKDAIVFVDNANTQRGNGAGGETILNYKNRKLYTMANAFLLSHPYGHKRIMSSFAFTNTDHGPPTNKDDTIRSPSFYDMDEENEDNDGAAESQNLLKHGQCRNESGWVCEHRWTAIVNIVQMANIVKDEPIIKFESNGPDRIYFCRGDKAFIALSNDPVEEFNKTIGTCLDEGIYCDVISGGVAKNGQSCTGLEIHVDKDKSARIIIPKDDENGVILIHKGAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3M9</accession>
    <name>T1H3M9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3M9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010860-RA">
        <property type="protein sequence ID" value="MESCA010860-PA"/>
        <property type="gene ID" value="MESCA010860"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834083_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010860-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7792" checksum="72532AB4BF9FF9B3" modified="2013-10-16" version="1">MDRHMINPYSTITKLRKYNASQIKTRNTIERAFGLLKKRPVLRFTIRTKMETIQDILLATTILNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3U2</accession>
    <name>T1H3U2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3U2"/>
    <dbReference type="STRING" id="36166.T1H3U2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010930-RA">
        <property type="protein sequence ID" value="MESCA010930-PA"/>
        <property type="gene ID" value="MESCA010930"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1437839_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010930-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="20714" checksum="B56C74D245BBAF9F" modified="2013-10-16" version="1">MDPLGSICSSQHEGDILEEEADNEEDRDDVLDELNEIDEEEEEDKMCNSDVKSSPSEPTKRDISAVKRELSLNLDGVAISSAVGLSTKMAVDLTDISPGSASLILNKQTKAQLNVPQIQSPSNSIFTTTSAEDEMTSQSVTPSEFGYQHLNRQSNVISSLENSPNSYENLEVAFLNETRKLIPPPSPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H423</accession>
    <name>T1H423_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H423"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011022-RA">
        <property type="protein sequence ID" value="MESCA011022-PA"/>
        <property type="gene ID" value="MESCA011022"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443343_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011022-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10604" checksum="C6895408B3363257" modified="2013-10-16" version="1">MFHTFHDNYKLNNTTRPQPLKASQNIKESTPKPQNYMNDDVFVQITKCTWKLVLLAKNFVLKYKLSYVVGWSTVGKLPFMRSYISLVIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H459</accession>
    <name>T1H459_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02082892">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082893">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H459"/>
    <dbReference type="STRING" id="36166.T1H459"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011060-RA">
        <property type="protein sequence ID" value="MESCA011060-PA"/>
        <property type="gene ID" value="MESCA011060"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2819935_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011060-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7981" checksum="BC8520BC65F0B5A4" modified="2013-10-16" version="1">VLNPLDAICDIKRRDAICVSNLKTAKRVEKDILAERPDVKIFLPFRFYVYRPEDLFIPNTYNTYLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H463</accession>
    <name>T1H463_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CAQQ02064354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H463"/>
    <dbReference type="STRING" id="36166.T1H463"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011064-RA">
        <property type="protein sequence ID" value="MESCA011064-PA"/>
        <property type="gene ID" value="MESCA011064"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036849_6_0_1"/>
    <dbReference type="OMA" id="IDKPRIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF379">
        <property type="entry name" value="HEXOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="265" mass="30366" checksum="A39B1598E6ABD273" modified="2013-10-16" version="1">LKSTTSTTTTTTSAIKVTPKKVEIIKKTDPLKGIPTKHIYEKGHLAFNQQNLCPLSTFNGLDPVVTPQIKLFIFITSAPSHFDARLAIRQTWGNYANRRDISIAYFLGQSRNQTIEDKLDNENELYGDLIRGNFVDSYNNLTLKTISMLEWLDRNCNSPLPKFILKTDDDMFINIPKLLLFIDKHNAEKRTIYGRLAKKWKPIRNKNSKYYVSPSQFSSTVFPHFTTGPAYLLTSDLVHDLYEQCLEETYLKLEDVFTTGIVSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4C5</accession>
    <name>T1H4C5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDNF/GAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDNFR family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02125958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02125959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H4C5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011134-RA">
        <property type="protein sequence ID" value="MESCA011134-PA"/>
        <property type="gene ID" value="MESCA011134"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1312901_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF12">
        <property type="entry name" value="GLIAL CELL LINE-DERIVED NEUROTROPHIC FAMILY RECEPTOR-LIKE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="38"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011134-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="210" mass="23482" checksum="7CFCBFBED4FD3BA4" modified="2013-10-16" version="1">MDCMNAQEKLHPVCAQKSPDSMNSPNSNGIVYHAPPACHMVADLCKEDRECRSKLEFFEQACSVDSVTKKCAGRPSSCRQALIGILGTILRTNCACQGQDQQFLYRCLGWRKLLWVNPCVVEAQKDFHMKRLAELGLLLPTTTTTISTTSTTIATTRPTTRPPPPPXXXXXXXXXXXXXXXQDQPQDHLLHRQRFILGGLLQGLRLQRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4F3</accession>
    <name>T1H4F3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02186620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4F3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011162-RA">
        <property type="protein sequence ID" value="MESCA011162-PA"/>
        <property type="gene ID" value="MESCA011162"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624840_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011162-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9355" checksum="B3D520926744AE7C" modified="2013-10-16" version="1">MENLFNINYINQYKLKLCVETLRTKAQKHSKDIFRIEIRSWNKELGNTQHCPFCYSHMNTVLEYCTNKVFLAHLCVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4I7</accession>
    <name>T1H4I7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4I7"/>
    <dbReference type="STRING" id="36166.T1H4I7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011196-RA">
        <property type="protein sequence ID" value="MESCA011196-PA"/>
        <property type="gene ID" value="MESCA011196"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874312_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011196-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7471" checksum="2584996478BB82C6" modified="2013-10-16" version="1">MPEDLQKEISCIIGIHYPERVIDLKASLEKNKKSMHKLRDMLMGEGETPAHCRPSNDDEIRQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5B4</accession>
    <name>T1H5B4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5B4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011491-RA">
        <property type="protein sequence ID" value="MESCA011491-PA"/>
        <property type="gene ID" value="MESCA011491"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560871_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011491-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9072" checksum="964AA90C6611E83C" modified="2013-10-16" version="1">MTISGFFGLEFGCVHPVYSKSIGVLGNIHLERAALSLITDFACIHTGLQSGMVSKQEDHIRLMNLRTMADYSIKLRSCITIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5N1</accession>
    <name>T1H5N1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5N1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012014-RA">
        <property type="protein sequence ID" value="MESCA012014-PA"/>
        <property type="gene ID" value="MESCA012014"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3379671_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="34" mass="3778" checksum="204C51C2B9FDC8B1" modified="2013-10-16" version="1">ETISRIAQVDPTEIYGMKGLVQPKPLANNLVVWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5N4</accession>
    <name>T1H5N4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5N4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012017-RA">
        <property type="protein sequence ID" value="MESCA012017-PA"/>
        <property type="gene ID" value="MESCA012017"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_34_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12337">
        <property type="entry name" value="RRM1_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000420">
        <property type="entry name" value="serine-arginine protein 55 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF51">
        <property type="entry name" value="SERINE-ARGININE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012017-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="259" mass="29275" checksum="6717F48497AE7655" modified="2013-10-16" version="1">MVGSRVYVGGLPYGVRERDLEKFFKGYGRTRDILIKNGYGFVEFEDYRDADDAVYELNGKELLGERVVVEPARGTARGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVEFASLSDMKTAIEKLDDTELNGRRVRLVEDRRGQRSGGGRGRSRSSSSRSRSRSRRRSRSRSRRSSRSRSKSRSRSKSKSRSKSKSAEKSRSRSRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5N6</accession>
    <name>T1H5N6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CABIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5N6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012019-RA">
        <property type="protein sequence ID" value="MESCA012019-PA"/>
        <property type="gene ID" value="MESCA012019"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_480296_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025946">
        <property type="entry name" value="CABIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052281">
        <property type="entry name" value="GAREM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454:SF11">
        <property type="entry name" value="SERRANO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12736">
        <property type="entry name" value="CABIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CABIT" evidence="3">
        <location>
            <begin position="30"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="498"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="410"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="507"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012019-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="568" mass="62925" checksum="9F1098C58E8EF8D2" modified="2013-10-16" version="1">MAATDGQIILAASRFGDTAIYLKDFSKQPLPAVAKIVKGQHLNLGVPTLSNPSLQSTALFLSAGKKYQILAQPIKIKEGRKPTNVGPKVLIPETYPGYFELLSEDGRSTRCIDSVLELSKRRNLRVLVRDTFRCNNLNKTIHAGEILTTINDNGKYLQCKTLKDEILNLPLETKAKFSPIAKEDSISGVHSVKNLLLKRMPLVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKVLITNNCSGNNSPTNGNSPLNTAINPKDCLEPQSPLFHIRYKSLSSLQLTPPDSNSTTPISTTPKGQQLQPQHIHQQDILLKCNNMNNQLAKSASIGAAREGTLDSSRSGGRTSGDSKLPEKKTRRLSRPRSLSNLVWDLKPLSKEKSSKKKLYIHHFDHRQQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7C6</accession>
    <name>T1H7C6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 19">E3 ubiquitin-protein ligase parkin</fullName>
            <ecNumber evidence="5 19">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 19">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms an E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 19">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17 19">Belongs to the RBR family. Parkin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5 19"/>
    <dbReference type="AlphaFoldDB" id="T1H7C6"/>
    <dbReference type="STRING" id="36166.T1H7C6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012664-RA">
        <property type="protein sequence ID" value="MESCA012664-PA"/>
        <property type="gene ID" value="MESCA012664"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050804_0_0_1"/>
    <dbReference type="OMA" id="DPKWDIK"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902532">
        <property type="term" value="P:negative regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20340">
        <property type="entry name" value="BRcat_RBR_parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20357">
        <property type="entry name" value="Rcat_RBR_parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16627">
        <property type="entry name" value="RING-HC_RBR_parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21382">
        <property type="entry name" value="RING0_parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000008">
        <property type="entry name" value="E3 ubiquitin-protein ligase parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047534">
        <property type="entry name" value="BRcat_RBR_parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003977">
        <property type="entry name" value="Parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054694">
        <property type="entry name" value="Parkin-like_IBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041565">
        <property type="entry name" value="Parkin_Znf-RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047536">
        <property type="entry name" value="Rcat_RBR_parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047535">
        <property type="entry name" value="RING-HC_RBR_parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041170">
        <property type="entry name" value="Znf-RING_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22605">
        <property type="entry name" value="IBR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17976">
        <property type="entry name" value="zf-RING_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17978">
        <property type="entry name" value="zf-RING_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037880">
        <property type="entry name" value="Parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01475">
        <property type="entry name" value="PARKIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 19" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 19" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588779" description="E3 ubiquitin-protein ligase parkin" evidence="21">
        <location>
            <begin position="23"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="22">
        <location>
            <begin position="30"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="231"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="active site" evidence="20">
        <location>
            <position position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR037880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR037880-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012664-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="459" mass="52270" checksum="D72CFB95855EBA78" modified="2013-10-16" version="1">MEFIFEFFRSFLNAMLALLSFGKKTVKNTLNINVKTNDQVLNIELQKSRNLKKSLLLLGITVEEIKIIFAGKELTDSTTIAECDLGNMSFLHAIKPRRNVQGQGISTLTKKEDQPSKPLCETLPDLKLVNMRKAHFFVYCQCKKLCKGKLRVRCFKCKSGAFTVYKDPECWDDVLKPQRIHGLCEGNEESPCTGNDNGLPYAEFYFKCAEHSLGMEDDFAAPLKLIRINEQNVPCLACMDVSETILVFPCELRHVTCLDCFLQYCRSRLLERQFMPHPDIGYTLQCPTGCPDSFIDEIHHFKLLTPEEYDRYQMYATEEYVLQAGGVLCPRPDCGMGLLIEPDCKKVVCQNGCGFVFCRDCLQGYHIGECNPEVNQIDTTRSCEYSVNPNNAAEARWEDVSTAVTIKVSTKPCPKCRTPTERDGGCMHMVCTRSGCDFEWCWICQTEWTRDCMGAHWFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7G4</accession>
    <name>T1H7G4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7G4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012701-RA">
        <property type="protein sequence ID" value="MESCA012701-PA"/>
        <property type="gene ID" value="MESCA012701"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177213_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012701-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="6072" checksum="1ECE9F1E65C4DF42" modified="2013-10-16" version="1">GVIEEMPPHIKKVCLALENSNQLSNALSAYLKNENYKRTDVDHVFLRFGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA41</accession>
    <name>T1GA41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Attacin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02179318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA41"/>
    <dbReference type="STRING" id="36166.T1GA41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000086-RA">
        <property type="protein sequence ID" value="MESCA000086-PA"/>
        <property type="gene ID" value="MESCA000086"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177234_0_0_1"/>
    <dbReference type="OMA" id="MRALHIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576753" description="Attacin C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000086-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10620" checksum="63CB9DFC864B669E" modified="2013-10-16" version="1">MCNLIRVIFLCFLISLISATPLRQRRQIDFNIQADHDDKVGTDLIVEAMAKLWRSKTGNTQIDGTAKVIHQAYTGAQGNTKYSGKLHISKLDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAK3</accession>
    <name>T1GAK3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000261-RA">
        <property type="protein sequence ID" value="MESCA000261-PA"/>
        <property type="gene ID" value="MESCA000261"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631253_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="17937" checksum="EF941DBC6C5BD17F" modified="2013-10-16" version="1">WISGTNCPVPKISLDPAKRELGDAAIIILRGNLTEMVKNMENKKCPTSVHHKNGIEHKDKPKEKPKEKVKEAPKEPEPIIPAKYKVDSEKDSNLSIDEEDNENSAGSSGSNANDNMIIEPPTPMPRTSRNNSLADQSSSSDAEAPKPRPRTKTPAYKVPLFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAS5</accession>
    <name>T1GAS5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000335-RA">
        <property type="protein sequence ID" value="MESCA000335-PA"/>
        <property type="gene ID" value="MESCA000335"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657294_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577168" evidence="1">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8605" checksum="138515ADF7D5AC07" modified="2013-10-16" version="1">MLKFVLVAVLLTLATVTGKPSGLVVYPPQFHYPGFVAATSSNVFTRTHNGILTPYVATYPYYYGGYGYPYRYPYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBD2</accession>
    <name>T1GBD2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UNR family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBD2"/>
    <dbReference type="STRING" id="36166.T1GBD2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000564-RA">
        <property type="protein sequence ID" value="MESCA000564-PA"/>
        <property type="gene ID" value="MESCA000564"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280530_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12913:SF1">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12913">
        <property type="entry name" value="UNR PROTEIN N-RAS UPSTREAM GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CSD" evidence="6">
        <location>
            <begin position="92"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000564-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="24555" checksum="263342B56E0704C4" modified="2013-10-16" version="1">MLLRKSFSISLRLRGREYACNIIRLPPGSVIFEDVNDKILKGQVLKPLDQLNARSTTDPLPGRIRYRAADYSEVEVPFGDKDQPQPQNQQQVYKGFIAVIKENFGFIETCDHDEEVFFHFSNFQGNPNWLELGQEVEYTLSPNGNTSTSGNCLPAETRDLLQKGDLVTFKVDDVGRAVEVTAVRQKKRASVDSIKGQFGFLNYEVEEGKKLFFHMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBF6</accession>
    <name>T1GBF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02095949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02095951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBF6"/>
    <dbReference type="STRING" id="36166.T1GBF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000588-RA">
        <property type="protein sequence ID" value="MESCA000588-PA"/>
        <property type="gene ID" value="MESCA000588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055524_6_1_1"/>
    <dbReference type="OMA" id="HICEPET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF482">
        <property type="entry name" value="FI23944P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="24205" checksum="773EA096D293CB4A" modified="2013-10-16" version="1">MYLKILVFFHHKEPPITGISILTVGAIVQTKYHHYANFVGSNIWTFPVIFIIVGSVVTLIGILGCCGICKESSCLILTFALFVIIIFLVEFALGVIFVNHGNFEKMLQEQFNSTMNQYNDRPEYQDAWRLIQSEMDCCGIDKPDDWYTRINSQNLPSSCCKVINLQEASDCTAKHAHPVGCITRLKKVIDDNSMMLGIVVLVIFSIQIFFNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBH2</accession>
    <name>T1GBH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000604-RA">
        <property type="protein sequence ID" value="MESCA000604-PA"/>
        <property type="gene ID" value="MESCA000604"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087561_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000604-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="12797" checksum="0D803CC5559AC37C" modified="2013-10-16" version="1">MLTYSFNAALHSGEDSTNDGKVLPIGVSGSSHVSEDFAGSLSFFSEEWICNIWIHGSTEETNCSSQTITNDTGNYRFEGAAFKNTLNKFFFISLDLNWAHALGNYNILTVPGKVTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBN1</accession>
    <name>T1GBN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02063884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBN1"/>
    <dbReference type="STRING" id="36166.T1GBN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000667-RA">
        <property type="protein sequence ID" value="MESCA000667-PA"/>
        <property type="gene ID" value="MESCA000667"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2564994_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF54">
        <property type="entry name" value="CYP9F3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000667-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9215" checksum="650ECCDE47F9B3FB" modified="2013-10-16" version="1">MENQECQDKLREEIMEISGTLDGKPISYEAIAKMKYADCVISEGMRKWPAAGLLDRICTKPTVLHDPISGKDVYLKKGDNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC74</accession>
    <name>T1GC74_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC74"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000875-RA">
        <property type="protein sequence ID" value="MESCA000875-PA"/>
        <property type="gene ID" value="MESCA000875"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925271_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000875-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7342" checksum="2807156332AE9840" modified="2013-10-16" version="1">MHKRADLTISRFEASEITRVVELEFLRDKCFHSQRRTYAYETYKGAIHILYDSLRLSYIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCL3</accession>
    <name>T1GCL3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001029-RA">
        <property type="protein sequence ID" value="MESCA001029-PA"/>
        <property type="gene ID" value="MESCA001029"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645099_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001029-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="9016" checksum="7530D2639FDF8662" modified="2013-10-16" version="1">KLELRALASELLISRIQNKFQKIITNIQRASSLNLREEGFIKIPTFFTELLNYSDSRISPFSLTHPQYQRRILPSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCM9</accession>
    <name>T1GCM9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCM9"/>
    <dbReference type="STRING" id="36166.T1GCM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001047-RA">
        <property type="protein sequence ID" value="MESCA001047-PA"/>
        <property type="gene ID" value="MESCA001047"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_922187_0_0_1"/>
    <dbReference type="OMA" id="ECAHFQR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053010">
        <property type="entry name" value="SET_SmydA-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455:SF3">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 9, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="23"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001047-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="28920" checksum="975EA281BCB8539E" modified="2013-10-16" version="1">MVEKLSHLIDFHLGELKSSDQNWCLELSNVAGRGVFAAKDIKAGEEIFREYPLLIGPTARKPTVLNTCTICYKLLELDSFICKKGCGLPICDSCSESKQHIQECSLFRSWKPVNQEKAEPGVIRMLTVARGLFLNKYQKELLYDYKQIIVSVLNTNAFESLSKSEDHDILLRGLYPLSGIMNHECTPNTCHYFEKDGLIIVKATVDIKDRTEITNTYTKLMWSNMMRQFFLRMTKNFQCDCIRCKDPTNIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCP2</accession>
    <name>T1GCP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exportin-7/Ran-binding protein 17 TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02080562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCP2"/>
    <dbReference type="STRING" id="36166.T1GCP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001062-RA">
        <property type="protein sequence ID" value="MESCA001062-PA"/>
        <property type="gene ID" value="MESCA001062"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005409_2_0_1"/>
    <dbReference type="OMA" id="DCFHELC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000042">
        <property type="entry name" value="exportin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057947">
        <property type="entry name" value="TPR_XPO7/RBP17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF2">
        <property type="entry name" value="EXPORTIN-7 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25795">
        <property type="entry name" value="TPR_XPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exportin-7/Ran-binding protein 17 TPR repeats" evidence="8">
        <location>
            <begin position="356"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001062-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="853" mass="97602" checksum="3CAD41D2AA620420" modified="2013-10-16" version="1">MLQGLTLEQRIDIRSYILNYLATRPNLQNFVVQALVTLLAKLTKCGWFDSFKNEAIFRNILEDVKKFLQGSVEHCQIGVQILSQVVSEMNTIFEVDFNMSFSKNRKIASSFRDLQLYDIFLLSCSLLITTRDNSKNMNFMEEGQQTLISHVLCLTKNCLSFDFMGSSTDESSDDMNSIQIPVNWRPAFLDSNTIKLFFDLYDVLPNGIASHSLSCLVLMTSVRRSIFNNNERIKFLTHFVEGVKRILENLHGLSDPDNYHEFCRLLARLKTNYQLGELISVPCYPQAIELIAKFTVQSLQMWQFAPNSVHYLLSLWSRMVASVPYVKTTEPHLLGTYAPEVTKAYITSRLDSAPLIVRDHLEDPLDDLGMVQQQLEQLAVIERCEYEKTCTLLIQTFEQTARNFQEYLRNPASNAMDMTIYELQLTWLVYIMGSAIGGRMSFTTNDDQDSCDGELMIGVLQLMTLTDSRLPQAGCEKLEIAMLSFLEHVRKTHISDQTQKSKVYKRLSEVFGINDEQMLLSLINRKIITNLKFWGHSETIITKSLSLLTDLSLNPNFLRKMAKLEEVQFMLTHHTSEHFPFLGTNTTLNEMRSRTMFYKSLGRLLMFDLGEDEERFYTFMAPIANQFESLIALNDYSNQEAKKAIIGLARDLRGLAYALNSKVPYAMFFEWIYPDYTPVLLNAVEFWPLDPTVTTPVLKLFAELVNCRWQRLQGNESSPNGILLFSEASKLICIYGNRILNSSPPVHNIYPLRLKGIAVCFLILKHALSGNYVNFAVFKLYRDDTLNNVLNVTAKLILSIQQSDILEYPKLSAAYYALINCLAQEHITILASLEPQLFLYILESLSKGLVALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCV1</accession>
    <name>T1GCV1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02150555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02150556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCV1"/>
    <dbReference type="STRING" id="36166.T1GCV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001124-RA">
        <property type="protein sequence ID" value="MESCA001124-PA"/>
        <property type="gene ID" value="MESCA001124"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2043921_0_0_1"/>
    <dbReference type="OMA" id="ICETAIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13364" checksum="D3273850CC416FC0" modified="2013-10-16" version="1">GLFLNEGQKWSERRKAITPAFHFNILEDFLEIFARSADVLIDKFDTDKVVNVPPLFLMCAFDVICETSMGLKMNSQSECNVPYVKAVNNHLKLAVLITGEQVSSTIISSPATPALGESCIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDH4</accession>
    <name>T1GDH4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02046225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02046226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDH4"/>
    <dbReference type="STRING" id="36166.T1GDH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001361-RA">
        <property type="protein sequence ID" value="MESCA001361-PA"/>
        <property type="gene ID" value="MESCA001361"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_6_1"/>
    <dbReference type="OMA" id="WGATINN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF13">
        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001361-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="294" mass="32988" checksum="D378F143FCF48A52" modified="2013-10-16" version="1">MLLPTVGWKPNAQTRRHRRLDWYIFRTQRSCLGIALNNNATLAASGAADFTGKVWNALTGVELHSFQHKHIVKTVSFDATSENLVTGSNEKILRVFNLHNSQIDPEIYTGHAGPVKRAIFCRNDKCIISAAEDKTVRLWDRLTGKEVQRIDLPTNPNSLELSADKKVLTVASGSTVSFWEIDTLKKIKEIKVPTICYSASLHPEKHCFVFGGEDFKMYKYDYITGNEIESFKGHFGPVHSVRFSPDGELYASGSEDGTLRLWQTTVGKTYGLWKCTEPGELNNSISSPREVHAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE25</accession>
    <name>T1GE25_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE25"/>
    <dbReference type="STRING" id="36166.T1GE25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001582-RA">
        <property type="protein sequence ID" value="MESCA001582-PA"/>
        <property type="gene ID" value="MESCA001582"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1706284_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140082">
        <property type="term" value="F:SUMO-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049627">
        <property type="entry name" value="SLX8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094">
        <property type="entry name" value="ELFLESS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="103"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001582-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="154" mass="17420" checksum="913B6F959AE5E18C" modified="2013-10-16" version="1">MIRRQIRRNISSEDIDRFSRSIMDHIQRSTAESIMSSGQLDDLYSMDSQEDQNSVESTTDIISVSSSITISSQEDNECFIIEDPLEENIPVSSSQNKDCKPSCPVCLKSCVVPVSTLCGHIFCETCILKSLRIARKCPVCKRNMKGNAFHRIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEW3</accession>
    <name>T1GEW3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02123344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02123346">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEW3"/>
    <dbReference type="STRING" id="36166.T1GEW3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001885-RA">
        <property type="protein sequence ID" value="MESCA001885-PA"/>
        <property type="gene ID" value="MESCA001885"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_883654_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="152"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="240"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001885-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="315" mass="35227" checksum="B7037869FDA21347" modified="2013-10-16" version="1">MCSSVARWNCLIHRQLGLYYTDLGLNFVGYSDVCWIYSDIVNANKPKVLWMCFNLVPQFLEILSMKENKYSGYAPVPPQLIFCLESKKAFCWGKTFYIIINISFEKSFQKNCPIMTEVASRKQLKSINFIVSSVSERLEPITIINIKPRRVLKGHQAKVLCSDWSPDKRHIVSSSQDGKLLIWDAFTTNKEHAVTMPTTWVMACAYAPSGNSVACGGLDNKVTVYPITSDEDMATRKRTVGTHTSYMSCCIYPNSDQQILTGSGDSTCALWDVESGQLLQSFHGHTGDVMAIDLAPNETGNTFVSGFLNFTSLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF39</accession>
    <name>T1GF39_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02024144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF39"/>
    <dbReference type="STRING" id="36166.T1GF39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001968-RA">
        <property type="protein sequence ID" value="MESCA001968-PA"/>
        <property type="gene ID" value="MESCA001968"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_819621_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF12">
        <property type="entry name" value="KEKKON 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="37"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001968-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="339" mass="36650" checksum="D4E5FA6327445270" modified="2013-10-16" version="1">MLRENIPYSIPPTCQTPERLAGKTWDRIDLDDFACTPNIQSVEIKTNGVEGKNVTMSCYVDGVPDPNVRWIVKNRIIANLSGNGETVARPSIMSSSSSTTTSSQGRKAYVVNFIRNSSNLTILQVEMQDAGVYTCTAENKAGRVEASMTLAVSRKPPEAPLGVKIILLCVTIALLFVIGSSFAAVCFCTKRKKGKLKLWKSSSAPGSDSYEKIEMKRTKPDMHCGRRGIDNNSSSIGGAGAGEYLCTENGGIAVVNATTTASVVLKRNGDYRTVPTEDDEDNNDTTTSFSITQTHKKQKQHHHKIEYSKAASKGRAGGAGNSLIDKNTDLHIGRLIDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFA8</accession>
    <name>T1GFA8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002045-RA">
        <property type="protein sequence ID" value="MESCA002045-PA"/>
        <property type="gene ID" value="MESCA002045"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1857567_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002045-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15632" checksum="A5339513F1F0D806" modified="2013-10-16" version="1">MINISLARHLGVSGMKTNLILKWILGKTVTEQVESCSFPKCKQGQFKIDTGSIGNCLDSLPFRGIKKKLLPLPVFRGDVEEWPLFEPSFFKSNEAIQYSDLKNNMRLVQCLKGKAKEAIEGLLIHPHYVGEVLRKLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFN0</accession>
    <name>T1GFN0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176851">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFN0"/>
    <dbReference type="STRING" id="36166.T1GFN0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002175-RA">
        <property type="protein sequence ID" value="MESCA002175-PA"/>
        <property type="gene ID" value="MESCA002175"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_7_0_1"/>
    <dbReference type="OMA" id="YIIRMAC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002175-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="162" mass="18781" checksum="1AAF039381436168" modified="2013-10-16" version="1">MADVVWDDELAYLAELNLRKCHLSHDKCIHTYRFLDDIIETALNGWFREFNFIDSSFIDRPPLGRSDLVRWGHFLEVVLDRNTHVGCAVMTFTERQYEGYYIIRMACNYAALYDGSSPIYVKGQPASNCAFGSNPQYPGLCKKNEPFDINYDEVYGPRNDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFU6</accession>
    <name>T1GFU6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Down syndrome cell adhesion molecule-like protein Dscam2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02144543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFU6"/>
    <dbReference type="STRING" id="36166.T1GFU6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002246-RA">
        <property type="protein sequence ID" value="MESCA002246-PA"/>
        <property type="gene ID" value="MESCA002246"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_583027_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001155">
        <property type="entry name" value="Down syndrome cell adhesion molecule 4, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001258">
        <property type="entry name" value="Down syndrome cell adhesion molecule 4, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000017">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000104">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF100">
        <property type="entry name" value="FASCICLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="28"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="136"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="282"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="378"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="52467" checksum="9FCA73ED852BD36C" modified="2013-10-16" version="1">MSAENKLGSSEYSEVVQVTTLEEVPSGPPLNVRAEAKSSTEITVVWEAPDREHWNGILLGYYVGYQISTSPEDKEVNPTQGFNFKTVEVRSHFGGETVLNNLKKYSQYHLIVQAYTSQGSGPPSKEIAVQTLEDVPAHFSTKTKQISVAKGKQVHVQCNVQGDNPIDIKWKIQSTQQILDESLDSRYTIREQVLDDGMVSELGISHTYRQDTGIYICHATNAFGQDNMSIQLIVQEVPEQPKNLRINSQQSRSLQLTWSQPFAGNSPIEEYHIFYKQISVPPKIMPIQAMTNMLREGMRAAISCQILEGDLPVSFRWERNGKPLIGTGNEQFRRLDEYSASLVIDHISSEHSGNYTCIASNKAGTETFTVPLTVNVPPKWILEPKDSSAQAGQDVYLHCQSDGYPKPTITWKKAIGTSPGEYKDFLYEPSVQLFPNGSIYFKKITKESQGHFLCEAKNTIGSELAKSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG29</accession>
    <name>T1GG29_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG29"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002335-RA">
        <property type="protein sequence ID" value="MESCA002335-PA"/>
        <property type="gene ID" value="MESCA002335"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725090_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="7916" checksum="F0F726587055D11D" modified="2013-10-16" version="1">MPGKSLTDQLSTLRQKRPVSTTSTHTSYASISVKPLPSNELIWHSFNNHQALGNALSCVKAADRIFNSFNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGD4</accession>
    <name>T1GGD4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">LIM/homeobox protein Awh</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02017215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGD4"/>
    <dbReference type="STRING" id="36166.T1GGD4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002450-RA">
        <property type="protein sequence ID" value="MESCA002450-PA"/>
        <property type="gene ID" value="MESCA002450"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275060_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09379">
        <property type="entry name" value="LIM2_AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000023">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF127">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002450-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24393" checksum="BA09A148C9B43E50" modified="2013-10-16" version="1">NFRAKCSKCCRGISASDWVRRARELVFHLACFACDSCGRQLSTGEQFALMDDRVLCKAHYLETVEGGTTSSDDSNPDGQDLERIASVTGLSKRVTQVWFQNSRARQKKHIHAVREPEGNSFARHINLQLTYSFQNNPSNTIPLNGNGKGLMFPSHERQIFKSSLNQIFAKFGTTSSSPISPNSYQILAHRSLTYRSLCFISKHFGAAFNHLMKVISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS4</accession>
    <name>T1GGS4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002603-RA">
        <property type="protein sequence ID" value="MESCA002603-PA"/>
        <property type="gene ID" value="MESCA002603"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963456_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002603-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6901" checksum="D8AFFB6C129B8E36" modified="2013-10-16" version="1">MQAYSNYRGISVLNIAYKIFASIVYERLKAHVIRIIIQSAQYDSHHDIPPYHSIYNKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS6</accession>
    <name>T1GGS6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGS6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002605-RA">
        <property type="protein sequence ID" value="MESCA002605-PA"/>
        <property type="gene ID" value="MESCA002605"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388717_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002605-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10714" checksum="9D5657730E956FCD" modified="2013-10-16" version="1">MPSRKFEILSVTSLLDFVAYETSANCYFAHTSPFLADFLQDQPYYPANQVPAHPKQPHYQTVPLASVHPATGNSYEAHFNYNHNDRLISILMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHG3</accession>
    <name>T1GHG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02114007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002861-RA">
        <property type="protein sequence ID" value="MESCA002861-PA"/>
        <property type="gene ID" value="MESCA002861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199965_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12471" checksum="2F30C05E17C20279" modified="2013-10-16" version="1">MRKSQNMNKCNECEEYGNDSLRNCTDCLGSFHDSCHSDETISKKTRASSCPSCVRIYANAIKDRNQKIEAHKENRRNAKLELELENEGDDEISEEVDFQEDNIEFITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHJ0</accession>
    <name>T1GHJ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHJ0"/>
    <dbReference type="STRING" id="36166.T1GHJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002892-RA">
        <property type="protein sequence ID" value="MESCA002892-PA"/>
        <property type="gene ID" value="MESCA002892"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309204_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF21">
        <property type="entry name" value="NA[+]_H[+] HYDROGEN ANTIPORTER 2, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="10440" checksum="81A3CD92CF4839A2" modified="2013-10-16" version="1">MSVLGGCIAVLGSKAIEYGSAGALGCTMITFVASIGWKKQDPNVDNLPVAQTLDLLWKFFKPISFSLIGKEVNFAVLDGKLVGYGVAIIVVGSLIILIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHX3</accession>
    <name>T1GHX3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHX3"/>
    <dbReference type="STRING" id="36166.T1GHX3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003036-RA">
        <property type="protein sequence ID" value="MESCA003036-PA"/>
        <property type="gene ID" value="MESCA003036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870184_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7086" checksum="657A94E528DB032E" modified="2013-10-16" version="1">MSFHVEFSNENGNFKTSTGHSGSIRSILELDNENSFISASKDSTASSLRSEDDEKKLSHCQFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHY3</accession>
    <name>T1GHY3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003048-RA">
        <property type="protein sequence ID" value="MESCA003048-PA"/>
        <property type="gene ID" value="MESCA003048"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266826_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003048-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11700" checksum="F3EAABC1654A2F7B" modified="2013-10-16" version="1">MKLYLPCEDWICNPGLLEGSLVFEAHVSTTMLTSSNSNVIGTLFNFVQLMRKETWLHHADFNLSHVGSSSRSVRTFSSNSRFDNCPSPRNVLKLILSGERFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI37</accession>
    <name>T1GI37_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI37"/>
    <dbReference type="STRING" id="36166.T1GI37"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003104-RA">
        <property type="protein sequence ID" value="MESCA003104-PA"/>
        <property type="gene ID" value="MESCA003104"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1940316_0_0_1"/>
    <dbReference type="OMA" id="EYYEART"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042541">
        <property type="term" value="P:hemoglobin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="13"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003104-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="149" mass="16813" checksum="6EB5A20075816634" modified="2013-10-16" version="1">MKFILTKTFFLPIGFPVEHTPSHIVPVKLGDPLLCTKISDLLMQQYGHYIQAINYPTVARGEEKLRLAPTPFHTHDMIDHLITDMKKVWQQLGIPLNGPTCSKECSFCKDPQVFEYYEARTRDGKNKSGNCSTQVACGIPDCPRMMAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GID7</accession>
    <name>T1GID7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02072823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GID7"/>
    <dbReference type="STRING" id="36166.T1GID7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003211-RA">
        <property type="protein sequence ID" value="MESCA003211-PA"/>
        <property type="gene ID" value="MESCA003211"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375270_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003211-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="22464" checksum="72B7315CF40C9D3E" modified="2013-10-16" version="1">MWTYMMAAARGDRANSMLSNKRRTESESPLYSYGQRQFYGRSNPNRNPGFCPYPSYSNHQMYHHTSSTVAHTPNLFQNQSSLDDEEDTSLYSPLPIARPDGNDNGVNVTGTTINNTNANEDKTNINTNPVSNQSNANAKSNHIKQSSSMNLNSYGPDRKSIITLRQNNQNETDEDYDEDDDEELDEVGYLENGIRKHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIU9</accession>
    <name>T1GIU9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GPI alpha-1,4-mannosyltransferase I, catalytic subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">GPI mannosyltransferase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphatidylinositol-glycan biosynthesis class M protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGM family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02389542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIU9"/>
    <dbReference type="STRING" id="36166.T1GIU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003384-RA">
        <property type="protein sequence ID" value="MESCA003384-PA"/>
        <property type="gene ID" value="MESCA003384"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326658_0_0_1"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577401" description="GPI alpha-1,4-mannosyltransferase I, catalytic subunit" evidence="15">
        <location>
            <begin position="29"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003384-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="11731" checksum="83233C2DAA4A0CB1" modified="2013-10-16" version="1">MLMDYIRRISFFKHLLIALGIRLVLVSYSEIHDQNAEVLYTDVDYEVVTDGARHILENRSPFQRHTFRYSPILALILTPNVYHKSFGKILFSLFDIVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ07</accession>
    <name>T1GJ07_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02005927">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02005928">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ07"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003446-RA">
        <property type="protein sequence ID" value="MESCA003446-PA"/>
        <property type="gene ID" value="MESCA003446"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239669_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003446-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11920" checksum="74CC1A4247713C8A" modified="2013-10-16" version="1">MAATERTNETFRGRSDPLHHTTSPNEICPLERLNIDMHPSLLSLPIKRSKIRKQETSLAHRSVTPTLEETLENYARILDETSPEEDFSTCNFGASGSSNEKEENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJP2</accession>
    <name>T1GJP2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJP2"/>
    <dbReference type="STRING" id="36166.T1GJP2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003692-RA">
        <property type="protein sequence ID" value="MESCA003692-PA"/>
        <property type="gene ID" value="MESCA003692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_7_1"/>
    <dbReference type="OMA" id="MIINEPL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13228" checksum="85D6A1E99D31DBED" modified="2013-10-16" version="1">MRILKKVFNETLRKHSVVSCIVRKSSTHFCVPNSNIIIEKDTIVLIPVDAIHNDPEIFSEPERFEPERFSTEELQRLNPCAFLPFGDGKRVCIGVGLAKLICKIGLVMLLGRFKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJY4</accession>
    <name>T1GJY4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02120743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJY4"/>
    <dbReference type="STRING" id="36166.T1GJY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003794-RA">
        <property type="protein sequence ID" value="MESCA003794-PA"/>
        <property type="gene ID" value="MESCA003794"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_1_3_1"/>
    <dbReference type="OMA" id="YWTPNIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000005">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein MutS core" evidence="8">
        <location>
            <begin position="375"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="723"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="541"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003794-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="105979" checksum="D95EC18BCCB75134" modified="2013-10-16" version="1">MAFDEAGFLGTLKFLEPDVIKDKKGRKKSDPDYDPSTLFVPEKFLNEQTPGMRQWWELKSNNNDSVLFFKVGKFYELYHWDADIGVQELGFAYMRGDFAHSGFPEAAFDKMASTLIEKGFKVARVEQTETPDMMTARCKKMGKTTKFDKVLLEGHEPFYMMSIVENVNEDESSTSSYGVCFIDTSIGDFYIGQFRDDKSSSRILTLLSHYSPVFVIYEKSAISERTQQIFKSVLCNVVKENIPGNQFWDGSKALKYLAENFYTHSREDGKEIWPETLKVMQDEGDHLGLTPKNIFKLALKSLGGILSFSVKCKIAEKVLNMAQFQLYIPPDENVSEDENITRILETTKDKIDNLNLKKKNMVLDSVTLQNLKIVEGENSLLNTIDLCCTNFGRRLLHHWVCSPSTDIKVIRQRQEAIKELILEPEILLEVRTFLNTLPDFERGLAQINVYGNPLIAKGHPDSRAILFEEKKFNNNKIQNFIAMVKGFESLMTLSTTFASFKSTLIKKITKESFPDLSKVLKFFQTAFDPSEAEKNGSIVPKKGVDQEYDDIEEEMENLNKELSDYLKEQEKFFGCRVTYYGSDKKRYQLEVPDKNAVKAGKQYVLESTKKGVKRFVTPETKEFLRRMMQAEENRANVVKDLSRRIYEKFSTNYNLWKQAMDCVSILDILCALADYAKNLNVFCLPEFVEDAEKPFIELEEGYHPCYRDDDFIPNGLTMGGDLAPLSILTGPNMGGKSTLMREIGLIIILAHIGSYVPAESCKLSVVDRIFTRLGAQDDLLAGHSTFMVELSETSVILKHATENTVVNFLADLQCRCLFSTHYHNLVDYFHGDKRVTFGHMACMVENDETDNPTEETVTFLYKYASGSCPKSYGFNAAKLAGMSNDIINKAYKLSKHMESIGLKRKIKSKLIEGGTPEEIKDLLKLLKACAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKD2</accession>
    <name>T1GKD2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKD2"/>
    <dbReference type="STRING" id="36166.T1GKD2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003954-RA">
        <property type="protein sequence ID" value="MESCA003954-PA"/>
        <property type="gene ID" value="MESCA003954"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163459_3_1_1"/>
    <dbReference type="OMA" id="MIISWNK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250">
        <property type="entry name" value="CHYMOTRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250:SF50">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588374" description="Peptidase S1 domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003954-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="7756" checksum="DB2521D8A5CAD70A" modified="2013-10-16" version="1">MWFGLSIFAILLVGSFALPGRIVGGHDAAPGQFPYQISLRNRNSHTCGGSILNERWILTAAHCVVFDGVGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKG1</accession>
    <name>T1GKG1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02112265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKG1"/>
    <dbReference type="STRING" id="36166.T1GKG1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003984-RA">
        <property type="protein sequence ID" value="MESCA003984-PA"/>
        <property type="gene ID" value="MESCA003984"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055524_12_0_1"/>
    <dbReference type="OMA" id="GYVKHTD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003984-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14191" checksum="9EC90D99DCDED6EC" modified="2013-10-16" version="1">MAKYKEDPTIGSLWDYTQREFSCCGTNSYKDWKLGSIDLNNYTVSKVPMTCCDMPQQQIGVFYCVALNGDEHLNGDGCLSEFSNYISQHAVSLGAAGVVIAVIQFFGVLFACYIAREIKIRDGISGFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKG9</accession>
    <name>T1GKG9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKG9"/>
    <dbReference type="STRING" id="36166.T1GKG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003992-RA">
        <property type="protein sequence ID" value="MESCA003992-PA"/>
        <property type="gene ID" value="MESCA003992"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_840174_0_0_1"/>
    <dbReference type="OMA" id="ANNGRWI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF295">
        <property type="entry name" value="AZ2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003992-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="331" mass="38267" checksum="DF8889B698AE1EB9" modified="2013-10-16" version="1">MQLRIIPISELKSKFYNIPFIEVEDDSILKIEKLYISDDGTVFGSTRKEFDFISIEEKEIEVNSELSSNSDHSEYLPTNDEDVESEKETNENSDSEKPDKENIDEFLLEPPRSKMESVINFLEDGKFECKECGKQSTFLGGLYRHTDSHLQRSTLCPVCGKTFKSAAILQYHLSSSDHSDLIPSEKPSKILCCQYSQSNMKNHRNRYKCSDEGLVFPAYANNGRWIKKKTDKPPTTKRKGKARKHINGFVCESCDLKFSSSSRLSIHNAMHHLEKKLNCPDCKQKFSTEFLLRRHLDKHLQFCKCQKCGKSVRRSYILIHEKKCGNNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKL4</accession>
    <name>T1GKL4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02074561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKL4"/>
    <dbReference type="STRING" id="36166.T1GKL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004040-RA">
        <property type="protein sequence ID" value="MESCA004040-PA"/>
        <property type="gene ID" value="MESCA004040"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2640948_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004040-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="9208" checksum="DB7119FB9A59DDF2" modified="2013-10-16" version="1">MTATSISRSEEEPSEKERWRRKKSSVSRFLILNVRDLLNSRANSVSKIEHQPRSWIETNFQKRDCVKFIPFPKDETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLT5</accession>
    <name>T1GLT5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02121588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLT5"/>
    <dbReference type="STRING" id="36166.T1GLT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004499-RA">
        <property type="protein sequence ID" value="MESCA004499-PA"/>
        <property type="gene ID" value="MESCA004499"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461109_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="17"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004499-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="9533" checksum="EA1175DC0195F7E2" modified="2013-10-16" version="1">MIKAIQEAGIPIDMVGGVSIGALMGALWCSERNITTVTQKAREWSKAIQEAGIPIDMVGGVSIGALMGALWCSERNITTVTQKAREWSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMU0</accession>
    <name>T1GMU0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004873-RA">
        <property type="protein sequence ID" value="MESCA004873-PA"/>
        <property type="gene ID" value="MESCA004873"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545204_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="14"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004873-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9011" checksum="390E128D87C2DB49" modified="2013-10-16" version="1">MSGGNDLVPGQPESKMVLIFNKGALAKQWELGRIVKAINGDDGFVRVVDVKTEGGIKRRAITQELIVSDMGLRREGINVLAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN77</accession>
    <name>T1GN77_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN77"/>
    <dbReference type="STRING" id="36166.T1GN77"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005021-RA">
        <property type="protein sequence ID" value="MESCA005021-PA"/>
        <property type="gene ID" value="MESCA005021"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725104_0_0_1"/>
    <dbReference type="OMA" id="TIRMYTH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005021-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8267" checksum="208FA515AB5DADAC" modified="2013-10-16" version="1">MKQKLSTNLINSIFLESNFVAWKMYEGLGTSDFLANVGGILGLFLGISVMSISEIIYFFTIRMYTHYFGRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNH6</accession>
    <name>T1GNH6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor L-glutamate and glycine-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02009862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNH6"/>
    <dbReference type="STRING" id="36166.T1GNH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005127-RA">
        <property type="protein sequence ID" value="MESCA005127-PA"/>
        <property type="gene ID" value="MESCA005127"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2910786_0_0_1"/>
    <dbReference type="OMA" id="MHLAKNY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="11">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005127-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7076" checksum="93767A50790C1401" modified="2013-10-16" version="1">MHLAKNYTGNERFFGFCVDILNRIANLVGFDYILDLVPDKKYGAKDPVTGEWNGMVAQLMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNV9</accession>
    <name>T1GNV9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02076416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02076417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNV9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005270-RA">
        <property type="protein sequence ID" value="MESCA005270-PA"/>
        <property type="gene ID" value="MESCA005270"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815363_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005270-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7687" checksum="EF8B3B78CCF00202" modified="2013-10-16" version="1">MLQVVAEHVNRILQEINTIGASYREQAMYICDVSSAVVSYWQQPFGPPTYPQKLIQVYDPKETIDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNZ4</accession>
    <name>T1GNZ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02043387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNZ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005307-RA">
        <property type="protein sequence ID" value="MESCA005307-PA"/>
        <property type="gene ID" value="MESCA005307"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107288_3_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857:SF43">
        <property type="entry name" value="GEO07291P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857">
        <property type="entry name" value="ODORANT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00708">
        <property type="entry name" value="PhBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005307-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="151" mass="17219" checksum="3BE61A9BDC5ACF12" modified="2013-10-16" version="1">MLPVNIKADIKSGKYQSYFIREITFTMKNIVLVAALGCFLFQMVEMNPELTKKCIEQEKVDIMEFQKIKNGKIEDPSHNFKCFNKCFMENIGFLVNGKLMEDKVLEALNQLPADKKDKTIAAFNKCKSKTGADDCDTAYQYMACMGENVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP11</accession>
    <name>T1GP11_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nav/unc-53 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02009706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02009710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP11"/>
    <dbReference type="STRING" id="36166.T1GP11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005325-RA">
        <property type="protein sequence ID" value="MESCA005325-PA"/>
        <property type="gene ID" value="MESCA005325"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001002_0_0_1"/>
    <dbReference type="OMA" id="VEWISKV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057568">
        <property type="entry name" value="CortBP2_NAV1-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039041">
        <property type="entry name" value="Nav/unc-53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057126">
        <property type="entry name" value="NAV1-like_ubiquitin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784:SF28">
        <property type="entry name" value="PROTEIN SICKIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784">
        <property type="entry name" value="STEERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25408">
        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23092">
        <property type="entry name" value="Ubiquitin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neuron navigator 1-like ubiquitin-like" evidence="3">
        <location>
            <begin position="48"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="CortBP2/NAV1-like AAA+ ATPase lid" evidence="4">
        <location>
            <begin position="305"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005325-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="49863" checksum="FB7AFB2900375A03" modified="2013-10-16" version="1">MSLLMFENIPDITDGKKISISVYLGQPEAFPKYYEEALENDRIYGDDDVKSYTNEANNEFIIAFTYISGKTTWQNLDYIVRKTFKDYLAKIDPGTNLGLNTDSITSYHLGDAKRGPEVGFPELLPCGYIIGNVRSLYICLQGVGSLAFDSLIPRSIVHRYISLLGEHRRLILCGPSGTGKSYLARRLADVDHKSSKDLQEYLGHIAEQASMANGASDLPSVIILDNLHHASALGDVFSCLLSAGPASKLPCIIGTMSQATCNTTNLQLHHNFRWVLTANHMEPVKGFLSRFLRRRLFTIDLTAPHLQPELQKVLAWLPTVWQHINRFLENHSSSDVTIGPRLFLSCPMDLKDSQVWFTDIWNYHLAPYIIEAVREGTQLYGRRGGAWNDPSTFIRDTYPWPYGPDSVPPLRGINAEDVGLEGCGNQNSDQPDPLMVFSCNLTDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP55</accession>
    <name>T1GP55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02055217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP55"/>
    <dbReference type="STRING" id="36166.T1GP55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005372-RA">
        <property type="protein sequence ID" value="MESCA005372-PA"/>
        <property type="gene ID" value="MESCA005372"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1210688_0_0_1"/>
    <dbReference type="OMA" id="ASACCRC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048663">
        <property type="term" value="P:neuron fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="105"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="107"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005372-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="22790" checksum="9C6E2F5E9DFF4105" modified="2013-10-16" version="1">RWCRREWSSPADARNNSDTNRRLYSQVFLGSPGDASNASIGSGEGTGEEDDDVNGKKNQKKRGIFPKVATNILRAWLFQHLTGGIALNGSGGGNFKPLPNTVNTQRHPYPSEDQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVYPPHAGPSGYGHDAMGYMMDSQAHMMHRPPGDPAFHQGYAAHYPTAEYYGQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP82</accession>
    <name>T1GP82_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02148481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02148482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP82"/>
    <dbReference type="STRING" id="36166.T1GP82"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005403-RA">
        <property type="protein sequence ID" value="MESCA005403-PA"/>
        <property type="gene ID" value="MESCA005403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014808_1_0_1"/>
    <dbReference type="OMA" id="EHIVFIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="12">
        <location>
            <begin position="414"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="569" mass="66207" checksum="E76C42A972EDF025" modified="2013-10-16" version="1">MSVEIPSGKKGPWFWGLPGLGIVTILAIFQKLDLSEVELTGIETIKSGYFKSLKNIESITINFADIRKLPEKLFFETCNLKSLYLYSNELEQLPEKLFKSLDKLLVLSLGNNKLRSLHENLFKPLISIENIDLSRNQLNTLNFKSFFEGLYKDGRSLIYNDQEYILSHGGGKRILDLTDNDVISSFHPKELNEDLKSFNITNRWQLKFGLENIDCYCQMYDINLFALSATAFQNLKFLEIDFIKCKNPTKKYLQYYIDSDFKSLIDPSECPGKCTESENRADCSNRGLTEIPRTLNTYYNQNECSYILIGNDWKCDCDNLTFIDLIRNTSQIRDEVKCLANEKYVISTKFDDDQIQEICTESSSGNIIIGLVLGIIFLTTILVTVSFKKELRIWLFAHRICLCWISEEEVDKNRKYDAFICFSEKDEYFVQQIVQELESGPNPYKLCLHFRDWLVGEYISKQIVTSVEDSRRSIFIITNNFLSSMWSRLEFRSACAASLREPIPRIIVILCEEIDELGKLDPEIKAYFNSNTYLKWDDNRFWSRLRYALPHRKSTISNDIELEELKLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP96</accession>
    <name>T1GP96_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head protein 9 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Kinocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the flagellar radial spoke RSP9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001032">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP96"/>
    <dbReference type="STRING" id="36166.T1GP96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005418-RA">
        <property type="protein sequence ID" value="MESCA005418-PA"/>
        <property type="gene ID" value="MESCA005418"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068343_1_0_1"/>
    <dbReference type="OMA" id="TFYHVPN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060091">
        <property type="term" value="C:kinocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055316">
        <property type="entry name" value="RSP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005418-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="281" mass="33488" checksum="18B10D101ABC740A" modified="2013-10-16" version="1">MNIEYLWEGLELISNECDQRITPEQHILIESSLLILQNEHRFKDIFFWGRIDAYERDYFIAFGIYADCLGERKFYYSTDCYNWIMMPYQNKELFEPCFLCKEMLLGDPTHVTVVKLDPKFSFDENQIILATEPEIVKLKEEDRLSFLVHHITDETAIVPRGAIYKLIDKTCIHNPLFRGLGELEIDDQSFYFQYRMPKIDYITNLAKKPDYNYCIDFLDTIDETFPKKRAYSLTMGYNERVSIIRSLIWPGMTFFHKLNSRKHGFAYFGSAKYNIDLLFMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPF6</accession>
    <name>T1GPF6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02033612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02033613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GPF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005485-RA">
        <property type="protein sequence ID" value="MESCA005485-PA"/>
        <property type="gene ID" value="MESCA005485"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046346_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="246"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005485-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="302" mass="34997" checksum="4334DA130C7B8D24" modified="2013-10-16" version="1">MKLNVSYPATGCQKLFEINDEHKLRVFYEKRVPMLTDXXXXXXXXXHKLRVFYEKRMGAQVEVDILGDEWKGYVLRIAGGNDKQGFPMKQGVLTHGRVRLLLKKGHSCYRPRRTGERKRKSVRGCIVDSNMSVLALVVVKKGEGEIAGLTDNNIPRRLGPKRASKIRKLYNLTKEDDVRRFVVRRPLPAKDNKKATSKAPKIQRLVTPVVLQRKRRRLALRKKRQVASKEAAANYLKLLAQRKKEFKVRREAAKRRRSSMRESKSSVSSEKKKKEFKVRREAAKRRRSSMRESKSSVSSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPX1</accession>
    <name>T1GPX1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipid-binding serum glycoprotein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02055710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPX1"/>
    <dbReference type="STRING" id="36166.T1GPX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005666-RA">
        <property type="protein sequence ID" value="MESCA005666-PA"/>
        <property type="gene ID" value="MESCA005666"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_967373_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038602">
        <property type="entry name" value="Mite_allergen_7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF13">
        <property type="entry name" value="FI04421P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005666-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="32315" checksum="23A0F442AC298CA9" modified="2013-10-16" version="1">MRAMHFIMFIIQNVKKNVEGMITLDEMLTKGRYTLSSFMSKSEGPFQVVLKNVTVTAKANLMVQMDGQLMTDSIDMDIVFKQMSMDFQNLGFMGSVFQSIMNSAPNLVFDGMKPFMLKEADTKMRSEIDGNIKKMMGDRRLPNSISPLDMAIAEGRKKVREMGYDPFHLPNYNKTVGVFSISTINTYITGASSFYRVGDIILSMENNTVSSKFLIGTQKIHGSTQWEVGMGIGLVSRIGHAQFSVEHIKVGVDVSQALDTRKHPQINDLQINLGNIQVRMDGLALWIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ21</accession>
    <name>T1GQ21_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ21"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005719-RA">
        <property type="protein sequence ID" value="MESCA005719-PA"/>
        <property type="gene ID" value="MESCA005719"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673916_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577544" description="Kazal-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005719-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9314" checksum="7EDFED0378DACB9C" modified="2013-10-16" version="1">MNIFLIFAIIFGTFALANSQDDKCSVQCALTLRPTCGFNGECYKMFDDVCDIKHYNTCLKKDGPEFAEVDNALCNGEQKKCEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ28</accession>
    <name>T1GQ28_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02031859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ28"/>
    <dbReference type="STRING" id="36166.T1GQ28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005726-RA">
        <property type="protein sequence ID" value="MESCA005726-PA"/>
        <property type="gene ID" value="MESCA005726"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071116_4_0_1"/>
    <dbReference type="OMA" id="KNCVYRP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="5">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005726-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="12613" checksum="7A6B852C39BB3F93" modified="2013-10-16" version="1">MANEEVQPCFKKSTFDQPLPGGKKKVWKGLKQILQTEKMLPWPEDAVTYSSLNPPPSFFPAKKYSDISGLIAPYTDPTTKLHYHNAEEFATVKQLPMDLTAGYLALREQVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQB9</accession>
    <name>T1GQB9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02035930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQB9"/>
    <dbReference type="STRING" id="36166.T1GQB9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005823-RA">
        <property type="protein sequence ID" value="MESCA005823-PA"/>
        <property type="gene ID" value="MESCA005823"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_1_2_1"/>
    <dbReference type="OMA" id="AGCTNRI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000073">
        <property type="entry name" value="Trypsin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588455" description="Peptidase S1 domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="32"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005823-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="20682" checksum="911D0F4E25E6C136" modified="2013-10-16" version="1">MLKLVSLLAVVAYASAGILPEFIRFPRLDGRIVGGSPTTIQQHPYQISLQYYGSHICGGSLISENQIVTAAHCLEGRTARTLQVRAGCTNRITSGVIVDVAEIKMNPSYDPYDMSNDIGIVTLATPVQFSDSIQPIPLARENPAPNTQAVTSGWGVLNEGSYGLPTELREVTVDIVSHEQCQKSYGNYDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQP5</accession>
    <name>T1GQP5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02102870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQP5"/>
    <dbReference type="STRING" id="36166.T1GQP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005956-RA">
        <property type="protein sequence ID" value="MESCA005956-PA"/>
        <property type="gene ID" value="MESCA005956"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_0_1_1"/>
    <dbReference type="OMA" id="RAQCRKN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="5">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005956-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="25242" checksum="62169D68476D94F4" modified="2013-10-16" version="1">MSSLKLQKRLAASVLRCGKKKVWLDPNEVNEIANTNSRQNIRKLIKDGLIIKKPVVVHSRYRVRKNTEARRKGRHCGFGKRKGTANARMPVKYLWMQRMRVLRRLLAKYRDTKKIDRHLYRDLYMKCKGNVFKNKRVLMEYIHKKKAEKQRSKMLSDQAEARRQRVREPVNAVKNVSPTRSKNSSLPALMKLLPKLLQESKHFTMSTFFTFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQV7</accession>
    <name>T1GQV7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQV7"/>
    <dbReference type="STRING" id="36166.T1GQV7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006022-RA">
        <property type="protein sequence ID" value="MESCA006022-PA"/>
        <property type="gene ID" value="MESCA006022"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_960722_0_0_1"/>
    <dbReference type="OMA" id="THPISRI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006022-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="32374" checksum="8FE2C1474AB4A265" modified="2013-10-16" version="1">MLKRLSYPLAWVEGLANETDVAGGVKKSEYDSAPNTGDSNQSVFSKVFSSSPIALVSELSGNLFSKTPSEDSGGSPIPPVTPHSTHSECFNTSGRSSIGTFIRPHSSEGTHPISRISKEKEKLSQPKLDYRSMVSIDDMPELFVSFDKLIPRPAKACQDPPLYLRLRMGKPKGKEIPLPTTIILENVNISYTKNLRVDELYRLIKTWVPHLYGELNEKEINERGFELIQNDTDWTKEGSNRNDKSDSNEDLVKLSTESWELIKAPYQKLFNVIKTVGSTDLDILGGEVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS11</accession>
    <name>T1GS11_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02050323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS11"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006458-RA">
        <property type="protein sequence ID" value="MESCA006458-PA"/>
        <property type="gene ID" value="MESCA006458"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_566946_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050975">
        <property type="entry name" value="Sleep_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562">
        <property type="entry name" value="ATILLA, ISOFORM B-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006458-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="482" mass="52612" checksum="DBFE31A1640D4C05" modified="2013-10-16" version="1">KNSYHFRSRSSFVLYYLLHEDCLGSHSEVTCSSVFADMAINYINMHIEEDIDDIQKGGEFECFTHVLNYTENYTQYRGCIYKGWDICSLHLKHSSNAEIGCLICDKDACNGDVSTDIDSGIIMTTNFPLESEETTDTVEDSTTTSDIPETSNESTPTTVNAIEQSTTSETTTTISSTTKLEEVTTTTIVVPETSEILPTTNEEIPNEGTTNYETTNHEEETSETTTSNMEETTESEVHDLQCFDSTWNKVSCNKINAKETLELMKSAGYNISSSFVQGSSFACISVDQTFANGDSLILKGCTFLNIDCTLPLNLEISASRTCKSCEEDLCNGVTSNFIGVLNFIVVIVVGKIFSNLSTVITITHPKMSVKIAITLFGVLSNTKTTVGAMAASGYNVSSLNSYPSYGCLRLNQTTNGQKFEIRGCTYANTTCSAPMKSGYTANAKDCKFCNTDLCNGVGANVINTSALIFTLVLGVFSKILIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSA5</accession>
    <name>T1GSA5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392593">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSA5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006557-RA">
        <property type="protein sequence ID" value="MESCA006557-PA"/>
        <property type="gene ID" value="MESCA006557"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834049_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006557-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7787" checksum="8ED27B0C4DD0C096" modified="2013-10-16" version="1">MISCFRSIRTKKRLHRQELNVRGNFVQLLPQPNDCWLSKERKFVSNSHFLTIDYGHNKVCPVTVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT08</accession>
    <name>T1GT08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02014045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02014046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT08"/>
    <dbReference type="STRING" id="36166.T1GT08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006826-RA">
        <property type="protein sequence ID" value="MESCA006826-PA"/>
        <property type="gene ID" value="MESCA006826"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_0_0_1"/>
    <dbReference type="OMA" id="KSPEINC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000010">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="172"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006826-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="284" mass="30988" checksum="DA7C162B720B60FE" modified="2013-10-16" version="1">MDNNVDMVICSAGTGGTIAGIGRKIKEALPSCKVVGVDPHGSILAQPEEINKTDVQSYEVEGIGYDFIPTVLDRSVVDEWIKVGDPEAFPMARRLNCEEGILSGGSSGSVLIGALQACKSLKAGQRAVIILPDGIRNYMTKFVSDNWMEARDFKPVVNEHNHWWWNHKASELKSFSPVVIDGSMTCQEAINLMKEKNVQHLPVVNNGKIKGCVTLDILTNQIVSNNLCIKSPATKGMFKKAIKVTADAIVGKVARILEVEDFVMIYKTENGAETVTGIFPVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT27</accession>
    <name>T1GT27_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NOP53 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02030742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT27"/>
    <dbReference type="STRING" id="36166.T1GT27"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006847-RA">
        <property type="protein sequence ID" value="MESCA006847-PA"/>
        <property type="gene ID" value="MESCA006847"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035888_0_1_1"/>
    <dbReference type="OMA" id="KPYDLWG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="446" mass="52433" checksum="E53AB030A72735E9" modified="2013-10-16" version="1">MLKIITESPNPLKGTKKKRVSKKNKSAWRKHTDVQDVEDFLESKRQEERIGQNRRRAVHIDTKAEEPQKLTPKQKRILHAKTAPLRSLAILENTSKVEDPIVKRNHVKKVKNGRNIEEEILNPRTQKHIEANKERKRYNEILDKKIKKKRNPLSKTFAVDLWEDTDPKDKYPVLKNKWISEDMAYYTLKNLNETGVIKVHDSLRHKTIKTKKRFEAPHPGTSYNPDPKDHIALLNKAVDKEEGIIKTAKHLKRITTEMFSKVTAFERDNNILKTKPEDNDLPYTSVNPPVVNKKKDKKARKNEKEQKLLAQELKKKKEAKKQLTDLHRLKHLDADVKKAGEELVELRKHRKKEAFKKKFETKRLGKLKFQEGEIDVNMKEDIAGNIRNIKTESNLLVDRFKNLQKRNIIPTSVDVGIRKRRKIKRFTRKSHKEPEPTILKCMKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTP5</accession>
    <name>T1GTP5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02174499">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02174500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTP5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007083-RA">
        <property type="protein sequence ID" value="MESCA007083-PA"/>
        <property type="gene ID" value="MESCA007083"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906680_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7537" checksum="904472E75CC78586" modified="2013-10-16" version="1">MAISKTSKKRLHLFSKTTIRCPHNQKQSYPNIQEQQERRMLCNARKLNFRYITRDVIFLKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU08</accession>
    <name>T1GU08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU08"/>
    <dbReference type="STRING" id="36166.T1GU08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007207-RA">
        <property type="protein sequence ID" value="MESCA007207-PA"/>
        <property type="gene ID" value="MESCA007207"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001030_0_0_1"/>
    <dbReference type="OMA" id="PGYEYNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19991">
        <property type="entry name" value="L 2 01289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19991:SF3">
        <property type="entry name" value="LETHAL (2) 01289, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="815"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="825"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="859"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="884"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007207-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="954" mass="110091" checksum="99E5C2A5AE9625F9" modified="2013-10-16" version="1">MYKPDCRKCAKALQELENIDDEADQLGIGFVKIHDEDLANEYNLGALPALVYYRHQTPIIYEGDEDDVIEDVTVKTLNTLISNIDNLVVLFYDHDDDDSMTVLEELEKIDDDCDKHGIQFVKIDDDKAESEYGIDSIPAIVYFEKQIPNVYDGDLMDEEQILHWLVGQLEKDEIEDVTDEMLDKMVKEGRVMAVLFYDNNDDYSQKVNICEIDNPEEALEYGINKVPKLIYFEKGIPTIYEGKLEDEEKLLKWLEEQTSSDQIEDVTDEMLDLIVEKMPYVAVLFYDKDQKKSQKVLAELENIDDECDQNDIAFVKIDDDAEAKEWGIEELPCIVLYERGIPHVYDGDLMKEDELLGWLVYQKRFSEIPEVTDEMKDKLVENTEHLAVIFYDKDDKQDMRILNELENIDDELEKEGIVIIRIDNAEEAKEYGLDHLPSLIYFENKIPSIYEGDLMNEEEVLEWLILQKKTATIEEVTDEILTELIKDHEYVVAFFTGPCEEGERCDNTLDALETIDDELDESGIIFVTTEDLVLAKKYNLKNLPQLVFFRNRDPMLYTGDLDDEDEVLAWLTDEKTLEIPGKIEEVNVKMLDKLLADNEHTVVFFLNKEGDKKSQKILNELENIDDDCEERDINFVKVSDDNIDKEYDLASLPALAFYRNKLRTVYVGDLMKEEEILEWVNELYDSNADVIESVDRKTLQVLINDVEHLAVFFYDDKCKECPEILAELENIDDDTDRYGITFVKSNDVKLAHEIGIFAFPSLVYYETGVPIMYDDDECFYVGLGHQGLGARKGQNFAPNNYQPFQCCPTKIQKTTKVPKMSARRIGYDKSDTKQEQKPSNTFKFAEETKKSSSKKSKSDDDDDDDDDDEPQQPLKVSYKKTHTNTKSNNDRPTSQTFTPVTSASKSTKPANTQNKGTKPSVDIKGNKKRAPAKDGKLNVNNGMFDDIVKKYMFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU91</accession>
    <name>T1GU91_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lipid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase CarF family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02017109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02017110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU91"/>
    <dbReference type="STRING" id="36166.T1GU91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007300-RA">
        <property type="protein sequence ID" value="MESCA007300-PA"/>
        <property type="gene ID" value="MESCA007300"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065233_1_1_1"/>
    <dbReference type="OMA" id="KFVWTCY"/>
    <dbReference type="UniPathway" id="UPA00199"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019547">
        <property type="entry name" value="Lipid_desat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052601">
        <property type="entry name" value="Plasmalogen_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48177:SF1">
        <property type="entry name" value="PLASMANYLETHANOLAMINE DESATURASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48177">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10520">
        <property type="entry name" value="Lipid_desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid desaturase" evidence="8">
        <location>
            <begin position="81"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="276" mass="31844" checksum="CE666BD9B0DC3C3A" modified="2013-10-16" version="1">MLEDDPNGNTETIKSNVSPRWGPQHKGAQELANLYSPGKRAQEIICVYTCIALMVVNFVFILRHFHLDRVSIAFLAAFCGIITADFGSGIVHWAADTWGSVELPILGKNFLRPFREHHIDPTSITRHDFIETNGDNFMVAIPILGVLFYNFLTKTPENIQQDFGWISYLFLCSIFVAMTNQFILMTYTSLLLDPQMVTYVCALPKWVLFLQNSHIILPRRHHRIHHVAPHETYFCITTGWLNWPLEKLKFWSSMEVAIEYLTGCKPRDDDLKWAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUH9</accession>
    <name>T1GUH9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007392-RA">
        <property type="protein sequence ID" value="MESCA007392-PA"/>
        <property type="gene ID" value="MESCA007392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888146_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="135" mass="16252" checksum="7DAD0A378F1B0D14" modified="2013-10-16" version="1">MGLSKACSEVKWIRMLMKKNSLLSKLIPNNMSLEKGFMGAKTLSFCIYCRKIPPGFFRFHWYSTFCQRLPFSWGHKFSQNNEYKHFINSHTQFSENQNHYSSSQKKEIWDELETIEYREPHHKGFSIWVEVNFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUI1</accession>
    <name>T1GUI1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Cytosolic glucose-6-phosphate dehydrogenase that catalyzes the first and rate-limiting step of the oxidative branch within the pentose phosphate pathway/shunt, an alternative route to glycolysis for the dissimilation of carbohydrates and a major source of reducing power and metabolic intermediates for fatty acid and nucleic acid biosynthetic processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:15842"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="4"/>
    <dbReference type="EMBL" id="CAQQ02196135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUI1"/>
    <dbReference type="STRING" id="36166.T1GUI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007394-RA">
        <property type="protein sequence ID" value="MESCA007394-PA"/>
        <property type="gene ID" value="MESCA007394"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1898638_0_0_1"/>
    <dbReference type="OMA" id="VCKMIST"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="10"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007394-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="134" mass="15533" checksum="2082D1BF252151B1" modified="2013-10-16" version="1">MNDQGNTLFIIFGASGDLARRKIYPVLWYLFRDQLLPPGTRFIGYSRSVVDKKTLAEKSKPFMKITGEEKVNLDDFWALHSFVSGSYNQDADYQKLETYLRSFGESNRIFYLALPPSVFEDVTKGIRHFCMVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUI9</accession>
    <name>T1GUI9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
            <ecNumber evidence="6">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02038927">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUI9"/>
    <dbReference type="STRING" id="36166.T1GUI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007403-RA">
        <property type="protein sequence ID" value="MESCA007403-PA"/>
        <property type="gene ID" value="MESCA007403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067275_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF43">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13375" checksum="F1C6387D3339BB38" modified="2013-10-16" version="1">MRGNLTSGITNLITSPQVKKAEWSNAVEALTDALELEAEVTKSIRGVIETCETGKEAKYNDYHLVDYLTGVYLEEQYKGQRELAGKLSTLKKMMTTHGEIGEFLFDKECKELTQHLSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUP3</accession>
    <name>T1GUP3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02145295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUP3"/>
    <dbReference type="STRING" id="36166.T1GUP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007461-RA">
        <property type="protein sequence ID" value="MESCA007461-PA"/>
        <property type="gene ID" value="MESCA007461"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751811_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="103"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007461-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17064" checksum="63B2BD092F1B5D18" modified="2013-10-16" version="1">MNVVRPNHMINLVLIDSDRNSNLNSNVFPQIGDGPRKCPRYWADDEIQYDHILVKYMQSESCPYYTRREFNVTNCKIDDTIKVTQFQYNGWPTVEGEVPEVCRGIIELVDQAQKHHAAENNFSIPTPIAVHCSLGTDRSSIFVAMCILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVD7</accession>
    <name>T1GVD7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02168913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007733-RA">
        <property type="protein sequence ID" value="MESCA007733-PA"/>
        <property type="gene ID" value="MESCA007733"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963446_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007733-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6727" checksum="EAF366CB123C14A0" modified="2013-10-16" version="1">MLLTSLYSAEFGSHRWRSSCCNEATGSREAALHELSPRMEENFKLSLGTAFHIFSNLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVF5</accession>
    <name>T1GVF5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02121756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVF5"/>
    <dbReference type="STRING" id="36166.T1GVF5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007754-RA">
        <property type="protein sequence ID" value="MESCA007754-PA"/>
        <property type="gene ID" value="MESCA007754"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055524_6_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF521">
        <property type="entry name" value="IP01817P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007754-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23854" checksum="8D27DBA4CB4CF0E0" modified="2013-10-16" version="1">MRHSPNHIWRVAILQHSLPGRFQGCTRNPTNSNRSDCPRNSGAIDFILWMLRCHSESYCMSMTYSILLFILMIAQLTLVVYMFIAKEDYLKHMEHVVDRAWDRRTQKADYMDGLQIGFKCCGNRDYRDYTYQGFVPATCCENPGHCSVENAYKNGCKSTFVNFWDKNSDIIKYAGLIIAAIEFVGFIFSCCLANNIRNYRRRSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW28</accession>
    <name>T1GW28_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-glucuronosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02151985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW28"/>
    <dbReference type="STRING" id="36166.T1GW28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007997-RA">
        <property type="protein sequence ID" value="MESCA007997-PA"/>
        <property type="gene ID" value="MESCA007997"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_992366_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="32227" checksum="6F336808F1FA98A4" modified="2013-10-16" version="1">VLWKWDATAPVPGNSPNILYKPWTPQDDILAHPNVKMFISHGGQGGVVESQYHGVPLLVIPFFGDQKVNRDSVESQGFGRGINFNEIDEESFKKLVLEVLENPSYSQKIKNFSKLYRDRPMTAKQTAIYWVEYVLRHKGAPHLQIIPFFGDQKVNRDSVESQGFGRGINFNEIDEESFKKLVLEVLENPSYSQKIKNFSKLYRDRPMTAKQTAIYWVEYVLRHKGAPHLQSPLVHLNFLERNSLDVLAVIFTVLALIGFILFASLKFIVKKLCGSKKHKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW64</accession>
    <name>T1GW64_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW64"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008036-RA">
        <property type="protein sequence ID" value="MESCA008036-PA"/>
        <property type="gene ID" value="MESCA008036"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2429596_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10863" checksum="A92174A95037D19E" modified="2013-10-16" version="1">MAVLILMLPLSFFYANTKDYKSYRGRSYDIDQENIVRICFISEEVSLLVQLGIHIYLLRVVKSHYTKKETKRNPNDQLNEIELKNILYFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWG5</accession>
    <name>T1GWG5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase VIIa family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWG5"/>
    <dbReference type="STRING" id="36166.T1GWG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008142-RA">
        <property type="protein sequence ID" value="MESCA008142-PA"/>
        <property type="gene ID" value="MESCA008142"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188593_0_0_1"/>
    <dbReference type="OMA" id="MSYKNVA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002082">
        <property type="term" value="P:regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.91.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7A1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.91.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036539">
        <property type="entry name" value="Cyt_c_oxidase_su7a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003177">
        <property type="entry name" value="Cytc_oxidase_su7a_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81419">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008142-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="8613" checksum="963D6667628DE11F" modified="2013-10-16" version="1">MNVTATGIRRLATKAADIPKGYTEIKKVQEQFQKKDGKPIFLKGGAFDQVLYRLTMGLSLVGVAGIGKLVYDLSVPKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWW6</accession>
    <name>T1GWW6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">POU-specific domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02061247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWW6"/>
    <dbReference type="STRING" id="36166.T1GWW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008300-RA">
        <property type="protein sequence ID" value="MESCA008300-PA"/>
        <property type="gene ID" value="MESCA008300"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013065_3_4_1"/>
    <dbReference type="OMA" id="RFEQSSH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF5">
        <property type="entry name" value="POU DOMAIN MOTIF 3, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POU-specific" evidence="5">
        <location>
            <begin position="13"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="20466" checksum="6FB1142C55799C70" modified="2013-10-16" version="1">DEPTINQATSNVVDGIDLDEIKEFAKAFKLRRLSLGLTQTQVGQALSVTEGPAYSQSAICSALAAQMYAAQLSSQQQNMYVNILKPKSEEWFEKLDITPKSAQKIKPVLERWMKEAEESHWNRYKSGQNHLTDFIGVEPSKKRKRRTSFTPQALELLNAHFERNTHPSGDYYYTDHIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX25</accession>
    <name>T1GX25_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02007522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX25"/>
    <dbReference type="STRING" id="36166.T1GX25"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008363-RA">
        <property type="protein sequence ID" value="MESCA008363-PA"/>
        <property type="gene ID" value="MESCA008363"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2801344_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008363-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="8092" checksum="8A0DFCD9397A3C4C" modified="2013-10-16" version="1">MSRLRLVPRKTLVLDLDETLIHSHHMSMPRNTVKPGTPYDFTVKVTIDRHPVRFFVHKRPHVDFFLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXQ0</accession>
    <name>T1GXQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYVE zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXQ0"/>
    <dbReference type="STRING" id="36166.T1GXQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008604-RA">
        <property type="protein sequence ID" value="MESCA008604-PA"/>
        <property type="gene ID" value="MESCA008604"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_977580_0_0_1"/>
    <dbReference type="OMA" id="ICKTELK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.730:FF:000005">
        <property type="entry name" value="RABaptiN (Rab effector)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003914">
        <property type="entry name" value="Rabaptin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015390">
        <property type="entry name" value="Rabaptin_Rab5-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31179:SF7">
        <property type="entry name" value="FYVE-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31179">
        <property type="entry name" value="RAB GTPASE-BINDING EFFECTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09311">
        <property type="entry name" value="Rab5-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103652">
        <property type="entry name" value="G protein-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE zinc finger" evidence="5">
        <location>
            <begin position="205"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="75"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008604-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="285" mass="33669" checksum="D8EB646542747BF5" modified="2013-10-16" version="1">MSKLIGDRESLNKHLETLQSDNEYLSGKYLSTSEELELEQINLPQTVVELTELVLKQQTDLIHSRVGCEFEKRKCVEFRDEAQLLRDRLNDVNKEYKTLEKKYQHTKKILEENIVTFKAKEQVLENDKDLLERKQSELNKQISEMRVHIIELQDTNEKLEKSNKDYKQKLKNLQEDLSNSEHVQKDFVKLSQTLQMSLEKFRTADTEVRWKDDDDVANCPNCNILFTVTVRKHHCRHCGETIEESSICKTELKKLGDEYNRLRAENLQLKEELSQKEESAGLHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYS5</accession>
    <name>T1GYS5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02374506">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYS5"/>
    <dbReference type="STRING" id="36166.T1GYS5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009012-RA">
        <property type="protein sequence ID" value="MESCA009012-PA"/>
        <property type="gene ID" value="MESCA009012"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_901046_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF41">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="36015" checksum="B1A104103B3DB916" modified="2013-10-16" version="1">MVRSVAAIVHIALAYVSVLINDLFVHADNLSISVYMVNEFPRSQVFSSIEFLGYSVKILETFADFINVYITFQFPGPKTNIEKSGQTSMIIESSPIDFTLSFDSLVQVSQNLHKVQKLSTVLEWMPWMIIVPVPKPLDFEYYIFKPFDAVTWVLTLLLIIACSFLESGEFWSNFCDFFRASLSQSYKIKSPSKWHLLFVIFGFIMTTWYCSLMGSFVTTTLYNEPLSTFEDIRKSGLKILIDDDDNILSQFPEIWKNRDVFKNVSRKEFFEYGNVLDTNYGYLEYGDRWEYYYKSRMNTNIEIYSGKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYT7</accession>
    <name>T1GYT7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYT7"/>
    <dbReference type="STRING" id="36166.T1GYT7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009024-RA">
        <property type="protein sequence ID" value="MESCA009024-PA"/>
        <property type="gene ID" value="MESCA009024"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874310_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009024-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7665" checksum="9158385340FEB761" modified="2013-10-16" version="1">MNYNIEILSINGRKCCLTNHYKEYMRSDLKFPVLEDDLWIVTFPKCGSTWMVEVAWLVQNNFDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZC3</accession>
    <name>T1GZC3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009222-RA">
        <property type="protein sequence ID" value="MESCA009222-PA"/>
        <property type="gene ID" value="MESCA009222"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1596389_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009222-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="167" mass="19447" checksum="CFF3DF132F716D2F" modified="2013-10-16" version="1">MISLVIQGFDLASLTWNWKICTRTTLTQLRSDWCRLLKNYQNRVDEAILDLCDDCHNTPHDVHHSLGINALKSQCIYHFQESTSIYQLFRDLKLLVKNWISLVHLGIMMTSNLKSIFFCLRQLEKNLPRETKLRPVKSLLGHNFTTSDKSIISSLMKEAFLSEDAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZC6</accession>
    <name>T1GZC6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-carbonic anhydrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02172157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02172161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZC6"/>
    <dbReference type="STRING" id="36166.T1GZC6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009225-RA">
        <property type="protein sequence ID" value="MESCA009225-PA"/>
        <property type="gene ID" value="MESCA009225"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2190490_0_0_1"/>
    <dbReference type="OMA" id="DTHSHIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF208">
        <property type="entry name" value="CARBONIC ANHYDRASE XA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009225-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="132" mass="15072" checksum="AD6FAF468D0D0B6F" modified="2013-10-16" version="1">MLVSWEDWWTYDGISGPAFWGLINPEWSLCNKGRRQSPVNLEPQRLLYDPNLRPLHVDKHRISGLIANTGHSIIFTAGNETVANYDGMQMPVNITGGPLSYRYCFHEIHIHYGLHDSFGSEHTVEGYNFPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZE1</accession>
    <name>T1GZE1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009240-RA">
        <property type="protein sequence ID" value="MESCA009240-PA"/>
        <property type="gene ID" value="MESCA009240"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388707_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009240-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10673" checksum="2A646961314EFA5D" modified="2013-10-16" version="1">MVASEISKPHGHHFIVKITKKDLEKMNAMKMEVIVVSSHIKDHKEGSREDEGHENGDYKQLKILRTDIGFEDRTSEDRILYAAKNGKLKGSIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZT5</accession>
    <name>T1GZT5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02139819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZT5"/>
    <dbReference type="STRING" id="36166.T1GZT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009391-RA">
        <property type="protein sequence ID" value="MESCA009391-PA"/>
        <property type="gene ID" value="MESCA009391"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024950_1_1_1"/>
    <dbReference type="OMA" id="QKNCESE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000008">
        <property type="entry name" value="pickpocket protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009391-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="291" mass="33720" checksum="DD14D311E3F41EDC" modified="2013-10-16" version="1">MNRDPDSQREHPMKWSLQGGYSPDTDVTSFPYRVLGAGSRPGLFVVLQGIEKNMDFLCRGPVQGFKIHLHSPEMFQWLKPSMITTSDGIINYTPEGRQCYFNNERYLKYFNIYTQKNCESECLSNYTLKHCGCVKFSMPRTEDMKVCNETSIKCYDEAEDELLKVEFSEGLIDSSFNHRGRTKCHCLPACTSLSYNTEISQADFDLINFVKSSESLYEYYQQFEGLKMSRLVIFFKESQFITSKRSELFGLTDFLANCGGLLGLFSGFSFLSMIELFYHCTIRLYHNMRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0F9</accession>
    <name>T1H0F9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02385785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0F9"/>
    <dbReference type="STRING" id="36166.T1H0F9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009637-RA">
        <property type="protein sequence ID" value="MESCA009637-PA"/>
        <property type="gene ID" value="MESCA009637"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="OMA" id="FKINSME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF54">
        <property type="entry name" value="CYP9F3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009637-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26919" checksum="AE7D888E9ADCAE9D" modified="2013-10-16" version="1">MRATLTPAFTGSKLRGMFELIRETAEESFNKLKDEMKNGVSDINVRDYFTRYANDVIATTAFGFKINSMEEKENYFFTMGQNVTNFGPFALAKCFILMNFKKLSNILKIRLISQKQQDYYTNLVMSSMRQRLEQKIFRPDMINMLIEASGKSVNSETKTALIKWTDNELVAQAFLFFLSGFDSVSTILCFIAHELMVNKDVQSKLKHEIQGVLKELKGESLTYDVLNGMKFMDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0L2</accession>
    <name>T1H0L2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02385413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02385414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0L2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009692-RA">
        <property type="protein sequence ID" value="MESCA009692-PA"/>
        <property type="gene ID" value="MESCA009692"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592486_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9376" checksum="EB2D0AFF08289A96" modified="2013-10-16" version="1">MERVNIPATNHIKSLAVLFEEIYLPLLSNEHNKELWPPTAAKDIQNQIKQMQRFLEETAQMMSTFYSFCISTNTNRDICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0Q9</accession>
    <name>T1H0Q9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0Q9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009745-RA">
        <property type="protein sequence ID" value="MESCA009745-PA"/>
        <property type="gene ID" value="MESCA009745"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173834_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009745-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="110" mass="12385" checksum="72205E9955F9439F" modified="2013-10-16" version="1">MPDKESSSNIEEQALGMASNTIKKVGNQFEMGLPWKDKTLQLQGSRPLAFPRFYVLNENSHTYGPHVDVCFTNLSVAVAPSTSCVSYTESSYVVIQVLDFIRVMHKRRLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H181</accession>
    <name>T1H181_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysozyme</fullName>
            <ecNumber evidence="2">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02172622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H181"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009935-RA">
        <property type="protein sequence ID" value="MESCA009935-PA"/>
        <property type="gene ID" value="MESCA009935"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2834077_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407:SF63">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="3" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577905" description="lysozyme" evidence="6">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009935-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7228" checksum="0643C829FFE32D19" modified="2013-10-16" version="1">MKAVILLFVLGVIGSSLAVIHDNCSLARELKNHDIPEEDIPTWVCLANHESGFNTHHVGVPDPSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1F2</accession>
    <name>T1H1F2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1F2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010011-RA">
        <property type="protein sequence ID" value="MESCA010011-PA"/>
        <property type="gene ID" value="MESCA010011"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2378729_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010011-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="95" mass="10219" checksum="D244354665AC860D" modified="2013-10-16" version="1">MGRANLNFGTKTSGAMSCLEVVNHPRTGLNRRNSRSEQQITGNGDILEIAEGTIAGAEDVIVTNVTIHVVGPNDEKGILMKGTNSNPIEFRIKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1S6</accession>
    <name>T1H1S6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1S6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010144-RA">
        <property type="protein sequence ID" value="MESCA010144-PA"/>
        <property type="gene ID" value="MESCA010144"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485896_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577316" evidence="1">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010144-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9899" checksum="5231867DD0A8AD65" modified="2013-10-16" version="1">MLFLRTLLVALSVSSIFAYPYCYRCASNWFDGCSPANTCLRDNYAGVVFSYPNRCGYVNGCLGRITNRYVEVDYSLCSSPYDVQYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Z4</accession>
    <name>T1H1Z4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GOST seven transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02027835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1Z4"/>
    <dbReference type="STRING" id="36166.T1H1Z4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010219-RA">
        <property type="protein sequence ID" value="MESCA010219-PA"/>
        <property type="gene ID" value="MESCA010219"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576605_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="7">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010219-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9608" checksum="63FB8E44B3B72B6A" modified="2013-10-16" version="1">MPPQIVLSTNPIRRIPDLWGFTNTYFYLIMCLVYVTYGIVWLTVSFLQWRDLLRIQFWIGGVIMLGMLEKAIFYAEKQSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H228</accession>
    <name>T1H228_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor L-glutamate and glycine-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02124999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H228"/>
    <dbReference type="STRING" id="36166.T1H228"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010259-RA">
        <property type="protein sequence ID" value="MESCA010259-PA"/>
        <property type="gene ID" value="MESCA010259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2910786_0_0_1"/>
    <dbReference type="OMA" id="VHIYLIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000210">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="11">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8417" checksum="2221B92E68D9CE00" modified="2013-10-16" version="1">MYIEKPYVMVKDDKNLTGNARFEGFCIDLLRSIAHQVGFQYRIELVPDSMYGVYDHEKQSWNGIVKELMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2R0</accession>
    <name>T1H2R0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2R0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010514-RA">
        <property type="protein sequence ID" value="MESCA010514-PA"/>
        <property type="gene ID" value="MESCA010514"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329230_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010514-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="207" mass="23083" checksum="7F8FABDF118CDCC9" modified="2013-10-16" version="1">MENKLMEVDKSTELHSKVNSSLLADPNNANKQLDEIDTIELKISEIVNQIDSKKSEVLDNTSTLNSGDVESNLIEVIETHDDEEEDNKARVALQQLEAINSSRRKKKPEIGSVVSTKKLHPATIDNNDLIAILSGTSVETTLDKHQTNGGSTEEVIIEGEGHFEVLEVEENETDKQKSFEETIEKKSDPKVDLVSKLAKDWSDDEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H305</accession>
    <name>T1H305_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02161049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H305"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010614-RA">
        <property type="protein sequence ID" value="MESCA010614-PA"/>
        <property type="gene ID" value="MESCA010614"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1268211_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="25507" checksum="70CDA55B1BFBD98A" modified="2013-10-16" version="1">MFTTSTTMFKAVDKSRTIILIFLWIYGYIIFNSFNSILCTFLTTLLYEPIIKTEEDLLKSSLKIIASKFRIVVFRDKLFKKFIETSTYEEFTSAMNLNTSFGSDIGSTCFKKSTCKGILFSPTSSSCKDKNGNLITDKQKVLKRSEQFFIELLIANNGTIFFQIPQQRRRHSTNKNCSGCWYRFQQWISQNSVKNLEQPEEWNKNIICYSQERRQVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H387</accession>
    <name>T1H387_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H387"/>
    <dbReference type="STRING" id="36166.T1H387"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010706-RA">
        <property type="protein sequence ID" value="MESCA010706-PA"/>
        <property type="gene ID" value="MESCA010706"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_522331_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052976">
        <property type="entry name" value="Scoloptoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933:SF40">
        <property type="entry name" value="CUTICULAR PROTEIN ANALOGOUS TO PERITROPHINS 1-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933">
        <property type="entry name" value="FI18007P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="312"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010706-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="522" mass="61507" checksum="A57C8ABB401657FE" modified="2013-10-16" version="1">EDNSNSDKVRGNERRNNRNHNSHKLASKENRNRDSDEPSKERHRFDDDRRSEKRHKPNNRRPVNYDEKPDSDSREIEDKPETPGVLVKPSVSSTSSLSSLFERPRAAPRIARPVPNNEKSKYSYSVTSSSTTTTEKPASEYEYYEDYETDQEHKEVTRDIKTSKKAEKEVEDDQDDLKEHSKTEEEKVPVNRDYKTNLRSVKRPFLPSRGGSPYLPRGLQPVGSQNNEYSTESTPIDMGSTISGVRLLEHGQPILRSQVEGSKFNGPRTTLPPQTNSKLKIEDLYETEYDVTLNDALNPTLKPLSADFLIMKCILYHHCLFGIRYDFLCANFTAFDQKTFICHFVSEVDCKNSPKYYHRNDALYMPASTTTLPPPTTTTTTTTTTTTPAPQRRPNRPGRRPLRRRPHYEDEYFDDDYYDDDYYEDRYRRRKQRPRQRRPDYDDEEEYDDRRSFSDDRYDRRPHKNRNDEYEEDSDSYEGRRKYNSKPRHRNRNYNSKVEDNSNQTRSEEMRDETIETIIATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3A2</accession>
    <name>T1H3A2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H3A2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010722-RA">
        <property type="protein sequence ID" value="MESCA010722-PA"/>
        <property type="gene ID" value="MESCA010722"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_864867_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990450">
        <property type="term" value="F:linear polyubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20337">
        <property type="entry name" value="BRcat_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20351">
        <property type="entry name" value="Rcat_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047540">
        <property type="entry name" value="BRcat_RBR_RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047542">
        <property type="entry name" value="Rcat_RBR_RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026254">
        <property type="entry name" value="RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041031">
        <property type="entry name" value="RNF31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LUBEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004">
        <property type="entry name" value="RING FINGER PROTEIN 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18091">
        <property type="entry name" value="E3_UbLigase_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010722-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="322" mass="36453" checksum="4A81B9AAE65369F7" modified="2013-10-16" version="1">LHGDAVGANEDEVLEYFSNLDIFLKNIVEPDVHELFQRKLRDRSLIQDPNFKWCIQCSSGFFARPKQKRLICPDCGSVTCAQCRKTWEKQHEGISCEQFEDWKKANDPDTQAEGVAQHLAQHGIDCPKCKFRYSLARGGCMHFTCVQCKYEFCYGCGKPFMMGAXXXXXXXXXXXXXXXXXXXXXXXKPFMMGAKCTVSSYCARLGLHSHHPRNYDVPSGEGAKAISRCPIPLQKETPNGLVDTICNGDAPNGHAGLCRNHFIEYLSLLIRENRLDTLPVLEVDDLETIVRRANKRLPPRPYGLLEGIYKNELLKIVRDQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3Y4</accession>
    <name>T1H3Y4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02194682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3Y4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010981-RA">
        <property type="protein sequence ID" value="MESCA010981-PA"/>
        <property type="gene ID" value="MESCA010981"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925261_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010981-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6574" checksum="9038C9294688CC1A" modified="2013-10-16" version="1">MLSSRNHSHHESLGPIFIIGDNDLVAICIRKKGAAANLWSSMENGGSGTTKNCTTYITIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4C1</accession>
    <name>T1H4C1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4C1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011130-RA">
        <property type="protein sequence ID" value="MESCA011130-PA"/>
        <property type="gene ID" value="MESCA011130"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588719" evidence="2">
        <location>
            <begin position="17"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011130-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="7636" checksum="3BCC1CBE213BB438" modified="2013-10-16" version="1">MKSFIVLACCLAMVASAPVADNSGVEIVRSDASVEPEGFNFVYELSDGTSHQEEGHLINTGSENAAIAVKGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4F7</accession>
    <name>T1H4F7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gamma-interferon-inducible lysosomal thiol reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02106897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4F7"/>
    <dbReference type="STRING" id="36166.T1H4F7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011166-RA">
        <property type="protein sequence ID" value="MESCA011166-PA"/>
        <property type="gene ID" value="MESCA011166"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066886_2_1_1"/>
    <dbReference type="OMA" id="AQMCKAY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF68">
        <property type="entry name" value="GH19763P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577356" description="Gamma-interferon-inducible lysosomal thiol reductase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011166-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18850" checksum="135567E7F48A6F39" modified="2013-10-16" version="1">MMNNIKVLFFFVVTFSNSFGQQKIDVTILYESLCPDSISFFRDQLRPNYEYFKNFININLIPFGKSQSFKNGGEFICQHGPAECELNRIQSCMLQNADNKDQQVYFAICQMSGRAEKTGKACAKRVGIPQNIDTCMDTESGTVLQLDAEQITSSFAPKFIPTIFTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4Q4</accession>
    <name>T1H4Q4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4Q4"/>
    <dbReference type="STRING" id="36166.T1H4Q4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011271-RA">
        <property type="protein sequence ID" value="MESCA011271-PA"/>
        <property type="gene ID" value="MESCA011271"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_984442_0_0_1"/>
    <dbReference type="OMA" id="GVEICCP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF398">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046017640" description="Amine oxidase domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="2">
        <location>
            <begin position="65"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011271-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="283" mass="31994" checksum="644D793B0FC8E008" modified="2025-10-08" version="2">MSLISFIILLLIHQLLAQECTIKDRDGVEICCPGFLKDQTNECVSKCKNVFDKTPRILIVGAGASGISAATRLLHHGFENVTLLEAQNRFGGRISTIPFGDSFIDLGAEWCHGQENNAVFELVKDKDLLQHSEPWDESYKLVRSNGEIVDDYTSKKLKGIFEKIQENKKDHLKNYNDSLGAYNKEYFLKALEEDTYATIDRDIAMEFLELFQKEIASAEGCKDVYDVSGKGNLQFKEAPGDQNMDWKGKGYVTVLNILMETGCRDLGILHKRILFNNKLQTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H558</accession>
    <name>T1H558_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H558"/>
    <dbReference type="STRING" id="36166.T1H558"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011433-RA">
        <property type="protein sequence ID" value="MESCA011433-PA"/>
        <property type="gene ID" value="MESCA011433"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178011_0_0_1"/>
    <dbReference type="OMA" id="ISTIDWY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019384">
        <property type="entry name" value="FHIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705:SF11">
        <property type="entry name" value="FHIP FAMILY PROTEIN CG3558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705">
        <property type="entry name" value="RAI16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10257">
        <property type="entry name" value="RAI16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011433-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="104" mass="11617" checksum="029209907CDE8C81" modified="2013-10-16" version="1">MALSKKNANIGTYIAKYSSMCPLLVTGLGGLYSRLPSSLEISTIDWYRITPDDVTDIPELTLFMNSLEFCNAVIQVAHDEIRYQLLDFLYQGFIVPVLGPAILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H587</accession>
    <name>T1H587_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H587"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011462-RA">
        <property type="protein sequence ID" value="MESCA011462-PA"/>
        <property type="gene ID" value="MESCA011462"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690665_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8428" checksum="B7B98067A23704B9" modified="2013-10-16" version="1">MLKHFLKLIGFFKPNGLVCIILFLGTLCSNNAYHSSVHCGEVQIFDAINLHAKTMILVHNLHWPLLHLVHRGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H658</accession>
    <name>T1H658_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H658"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012191-RA">
        <property type="protein sequence ID" value="MESCA012191-PA"/>
        <property type="gene ID" value="MESCA012191"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036945_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="366"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="424"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012191-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="488" mass="56409" checksum="A5B07CDFD6D64811" modified="2013-10-16" version="1">AWDTEQLEIFDLVQDINKNFYDLIGVSQNATIQEIKRAFRTLSIQLHPDKNDAEDANEQFRNLVSVYEVLKDASKREKYDKVLKDGLPNWKSALFYYRRMRKIGLMEGSLICFMIFTIGQYFFAWAAYLEKKYTAEEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGFWTDEDLVELVRLVKKYPGGTASRWEVIGEQMHRSVNEITFMAAKLKENGYRIPGHSESVAETIVQDQRKKKSRNKNTLVVAETNWSQEQQAALEAALMKYKKTAMLTWQKIGNSVPGKTKEECLQRYKYLCEVIKQQKAQKREEVVDEEPSCXRKKLENVEEAEEEVEEKKLEVPQKGTKKNKRKERRRRRDFSSGEDSDDMYTYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Q4</accession>
    <name>T1H6Q4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6Q4"/>
    <dbReference type="STRING" id="36166.T1H6Q4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012387-RA">
        <property type="protein sequence ID" value="MESCA012387-PA"/>
        <property type="gene ID" value="MESCA012387"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954220_0_0_1"/>
    <dbReference type="OMA" id="HGQGPHA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034751">
        <property type="term" value="C:aryl hydrocarbon receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039091">
        <property type="entry name" value="AHR/AHRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10649">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10649:SF12">
        <property type="entry name" value="SPINELESS, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012387-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14947" checksum="7880F051561776C8" modified="2013-10-16" version="1">SDIVHQSVYELVHSEDREELQRQLLWNSFLPADLSNISLGETLTQDKIQYLERSFTVRFRCLLDNTSGFLRLDIRGRIKILHGQNKKTEDPPMALFAYCTPFGPPSLLEIPQKENMFKSKHKLDLSLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H798</accession>
    <name>T1H798_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-soluble NSF attachment protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-ethylmaleimide-sensitive factor attachment protein gamma</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H798"/>
    <dbReference type="STRING" id="36166.T1H798"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012636-RA">
        <property type="protein sequence ID" value="MESCA012636-PA"/>
        <property type="gene ID" value="MESCA012636"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363409_0_0_1"/>
    <dbReference type="OMA" id="YANRACH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF2">
        <property type="entry name" value="GAMMA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012636-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="201" mass="22430" checksum="686F9C288819FE1C" modified="2013-10-16" version="1">KETNNQMKIEEYANRACHLYQQHGSPESGAAALDKAAKMVENEHPDVALRLYQHALEVVMIEDSTRQGGEYATKISRLTVKLGLFDQATDAIRREIGINQQTESYQQIGRLAVGLVLVQLARGDTVAAEKAFKEWGNYCDPPEVQTLEMLLQAFDDEDPEAAAQALNSPFIKHMDVEYARLARDLPLQGYLRGTKKAADNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA14</accession>
    <name>T1GA14_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA14"/>
    <dbReference type="STRING" id="36166.T1GA14"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000057-RA">
        <property type="protein sequence ID" value="MESCA000057-PA"/>
        <property type="gene ID" value="MESCA000057"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013883_0_0_1"/>
    <dbReference type="OMA" id="GEVCDTQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056978">
        <property type="entry name" value="CC4_RECK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056976">
        <property type="entry name" value="EGF1_RECK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056977">
        <property type="entry name" value="FnI_RECK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056979">
        <property type="entry name" value="FZ_RECK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039016">
        <property type="entry name" value="RECK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13487:SF3">
        <property type="entry name" value="REVERSION-INDUCING CYSTEINE-RICH PROTEIN WITH KAZAL MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13487">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23332">
        <property type="entry name" value="CC4_RECK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25027">
        <property type="entry name" value="EGF1_RECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25028">
        <property type="entry name" value="FnI_RECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23298">
        <property type="entry name" value="FZ_RECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kazal-like" evidence="1">
        <location>
            <begin position="407"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="1">
        <location>
            <begin position="480"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000057-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="707" mass="78365" checksum="4F851196DD0C9365" modified="2013-10-16" version="1">LPCCNLAPNSNCSKICKKTLESNENQEIILDEMEAGGCGPVLPHQPFWQCFFQEEKVTHKKPHNSFRSQEVSQINQIGLDSAKLHCCSKAISGKCKKLCLRTFSNDWTAARQEFDIDCLYEVKELSLRQCIDEVEEPCQLGCDGLSFCTNFNNRPTELFRSCNTNADLAARSDIEKIKELGFISLPECAKCAPEKWVSIACILQLKPCTRASHLNQICREDCYEILNECMDWTRMNVSVTAADICSTVSPSEEDAPCVSLKPYLKPSDVPVADNGGHHGLTSLCRHSSCNRSDVCIPNRNEGNRHQCAPSCSLGEASSYKVAVGSYVRVPVSSNDPGCFKICKCSANGRIEHCQPLPCFSYDSCYYSDRMIPHLTSFYIECNLCSCFAGELICTKKQCRIPGISEYSYTSLPCNCGPHYIPVCGKHNGITYPSACVAKCNGLQEENIMFGACRSRNPCRNGTQVCPSGTKCVEDYKTCLSVMHEPCNQYQCVDTSMDCTIKNQPVCDTKGKTFPSLCHLSKSRSELAYHGPCYKTCSMSEKVCGINGVTYKHECEAWSDFSLVDYNGKCMEIGLIASDMGPKCNYAKCPKLPVSNCVSVTPPGACCPICGKALRIVYSRKQVDRALYALKGKNLELLSLRSILKTLDGLLEFSECQLAGYSTIETDIFIVVTTRQKHPKSIQMEACSREAEKLKTLIENKGHNVMSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAF3</accession>
    <name>T1GAF3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02066728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02066729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000209-RA">
        <property type="protein sequence ID" value="MESCA000209-PA"/>
        <property type="gene ID" value="MESCA000209"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220895_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000209-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="25156" checksum="5AA6441078D8ADEC" modified="2013-10-16" version="1">MSGGTGGSAESMPYIFKNTNNHLNVSKHKATSISAECSPILLRHHQMQLERQQIIDAAISGKCYSDSMQLHVVDATGDVTTNIVPIFVDSQSSAAPVKQSSPSILKKHKQQPLQPDIVRYNSSSSSSENSVTIMKFNNARKSNLVDNLGVLPSWPSDGYDPQSAFEKKQRKYRNTGSDPGMVYDQLNEIISIPHNPSPLPYVYDMTLEVDSSKNELKTLVEKSHKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAL7</accession>
    <name>T1GAL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02200125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAL7"/>
    <dbReference type="STRING" id="36166.T1GAL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000276-RA">
        <property type="protein sequence ID" value="MESCA000276-PA"/>
        <property type="gene ID" value="MESCA000276"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533982_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000276-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9353" checksum="EE91CA2C87E346C5" modified="2013-10-16" version="1">NIFSLHQSFNISTANNLFKTLVVSGDAHIQLATCSLLTLTKLLVPMSPKFNNQFAYTKSDSGEGTSQNFDDNKNNELWNKSDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD86</accession>
    <name>T1GD86_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD86"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001267-RA">
        <property type="protein sequence ID" value="MESCA001267-PA"/>
        <property type="gene ID" value="MESCA001267"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457359_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001267-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10538" checksum="0AFF561C2E9B74AF" modified="2013-10-16" version="1">MSMTKLFAINEACQILSIKELKERKLSAKSIFRTKRCSDKEEEVFKQWEDLPKEILNYNEGKTPTQRNQFQEFPQQQNKAIRVSTITKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDE1</accession>
    <name>T1GDE1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02072206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDE1"/>
    <dbReference type="STRING" id="36166.T1GDE1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001326-RA">
        <property type="protein sequence ID" value="MESCA001326-PA"/>
        <property type="gene ID" value="MESCA001326"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208814_0_0_1"/>
    <dbReference type="OMA" id="FRCKAFI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF119">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001326-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7250" checksum="593C97D2192A0AE0" modified="2013-10-16" version="1">MGDFQCKRRSWRSDLPATSVIITFHNEARSTLLRTIVSVLNRSPEHLIKEIILVDDFSDHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDT3</accession>
    <name>T1GDT3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001479-RA">
        <property type="protein sequence ID" value="MESCA001479-PA"/>
        <property type="gene ID" value="MESCA001479"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069908_2_1_1"/>
    <dbReference type="OMA" id="MANDYWQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF18">
        <property type="entry name" value="BCDNA.GH05536-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001479-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="23578" checksum="9A09210841B98553" modified="2013-10-16" version="1">MQMFSMLKDGFPELNIPAIDPFIIEKAKYQIKSDDFQIRVVLKNMVVLGSSQTNIQDVNFEKSNNLIFMKTHSEIPSMNVTGSYKAEMTVSETTSYYGGNFRVELYDIVSVHEFDAELIDDGDRKIVKLLKFDVSPTIGKMNMKAAGFDPDPTINSMLVQMANDYWQQFYKFLVPETKKYWEPIMLEISNRFLSVIAFDSVLDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDW4</accession>
    <name>T1GDW4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDW4"/>
    <dbReference type="STRING" id="36166.T1GDW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001515-RA">
        <property type="protein sequence ID" value="MESCA001515-PA"/>
        <property type="gene ID" value="MESCA001515"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1290294_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035092">
        <property type="term" value="P:sperm DNA condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024460">
        <property type="entry name" value="Protamine-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06382">
        <property type="entry name" value="Protamine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001515-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24448" checksum="F81E1B7F26A9249C" modified="2013-10-16" version="1">MIVVDSCGARRRKPVCAKKRPKRSKSRSCKGSKRKKCAKPGPVIKNAFINFLRDFRKKRCGKSMPEISKEAAKRWKCMSICKRSKYIKQACKIGSCAKKPKRRKRTMASKCAKKRRPRRKASSCAKKRRPQVCIFIRGRTRRPYPPVITPLEISVNSFQEEFSGGVIGKVFASDLANTCQNPIRFSSISRVPSLQHFQGEWNFELPSQHRCGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GF91</accession>
    <name>T1GF91_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02129351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GF91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002023-RA">
        <property type="protein sequence ID" value="MESCA002023-PA"/>
        <property type="gene ID" value="MESCA002023"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560890_0_0_1"/>
    <dbReference type="OMA" id="WCIAEAQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588244" evidence="1">
        <location>
            <begin position="18"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002023-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="6762" checksum="261325A60E3E6D77" modified="2013-10-16" version="1">MKYFIIFLAFAVLSCNGQGGGGNAGAGAGAGFQAGAGAGAGGGGGGNGQGGAGGQGGAGVGAGFQAGASAGAGGGAGGGQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFD5</accession>
    <name>T1GFD5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002074-RA">
        <property type="protein sequence ID" value="MESCA002074-PA"/>
        <property type="gene ID" value="MESCA002074"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3002467_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002074-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="57" mass="6676" checksum="23BDD48D6981700A" modified="2013-10-16" version="1">MGNNNVVKRVFDAVPVSACRPRKPALRWKDSVMSNLKRLTPNIGEWRQIAANRRDWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFK8</accession>
    <name>T1GFK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02132870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02132871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFK8"/>
    <dbReference type="STRING" id="36166.T1GFK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002152-RA">
        <property type="protein sequence ID" value="MESCA002152-PA"/>
        <property type="gene ID" value="MESCA002152"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005350_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000003">
        <property type="entry name" value="Ecotropic viral integration site 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF22">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="88"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002152-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="39753" checksum="4E775850975AD4DC" modified="2013-10-16" version="1">MTLTTIAPTSTTTSQAKSESKMDVKLPSPTGSIVDESLPTSEMDLLAKLEAANKLIESDAKSLNSLHSTHSRKNSDTSQISCNSGGNSSGEEDNWTLWATIVNDWEGSLRRKNPCVRELATSACEKVIRRDIARTYPEHDFFKEKDGLGQEALFNVMKAYSLYDREVGYCQGSGFIVGLLLMQMPEEEAFAVLVQIMQQHRMRDMFKPSMAELGLCMYQLENLVQEQLPDLNIHFVQQAFQTTMYASSWFLTLYTTTLNLNLCCRIMDIFLSEGMEFIFKVALGLLTIGKEQLLCLDMEAMLKNSEMLAEGELRSNLDDSDKVRDLQDRLAEMKAEFPTPLSPDTEPWKWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFL4</accession>
    <name>T1GFL4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">N-terminal E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
            <ecNumber evidence="6">2.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].</text>
            <dbReference type="EC" id="2.3.2.25"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EC" id="2.3.2.25" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02027725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFL4"/>
    <dbReference type="STRING" id="36166.T1GFL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002158-RA">
        <property type="protein sequence ID" value="MESCA002158-PA"/>
        <property type="gene ID" value="MESCA002158"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_15_1_1"/>
    <dbReference type="OMA" id="HPICASI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000022">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002158-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15680" checksum="F9A7F0892892A9B9" modified="2013-10-16" version="1">MSLISEPPPGVTVDSESVSENLSEWKINIDGVEGTLYEGEHFQLLFKFNNKYPFDSPEVTFIGSNIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSISSMLSSCKEKKRPADNTIYVKTCNKNPKKTKWWYHDDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGE9</accession>
    <name>T1GGE9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02021330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGE9"/>
    <dbReference type="STRING" id="36166.T1GGE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002468-RA">
        <property type="protein sequence ID" value="MESCA002468-PA"/>
        <property type="gene ID" value="MESCA002468"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_864868_0_0_1"/>
    <dbReference type="OMA" id="RITEPLM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002468-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="322" mass="35219" checksum="E3203B64FDCE0CB6" modified="2013-10-16" version="1">MLAATTQNLLPILPFLLPNNMHSDAVMLQGHVSSSSPRLSSSVSPVKEDMDDGDRSCCSEDSVLSVGKEVGGNGDGDSSSCGGETKHDDENTSNFSVDEQSSLNQDFDVNSSEESMCQNNDSSLLMSSTTLRIPVPVVRPNPSRFQEEFIRKSQFYAEELMKQQMQFMAAARASAFTSLPTVGSCTSQSEFRVPNVESFHSHLNAISKLAAATNTPFVQCPSQSLNQLQQQLNHLPFINNNIHDQRLNKNSGAKKTNSSNKIKPMDLTSLTQEVNQTEVSSPISSPEQKTSKDSDSSSSKSSDPMVWPAWVYCTRYSDRPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGF3</accession>
    <name>T1GGF3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02070349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002473-RA">
        <property type="protein sequence ID" value="MESCA002473-PA"/>
        <property type="gene ID" value="MESCA002473"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3147471_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002473-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="49" mass="5649" checksum="ECAEC74F6227D897" modified="2013-10-16" version="1">MNDLVRMTNELGDPKEKIILPSYRDALNIRVTEELGQIRDGDGQVHDWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGF6</accession>
    <name>T1GGF6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002476-RA">
        <property type="protein sequence ID" value="MESCA002476-PA"/>
        <property type="gene ID" value="MESCA002476"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763435_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="16785" checksum="3F466CCBB97974F7" modified="2013-10-16" version="1">MYPDEYHMRSWPESDLPRDYDWHSRKPLKDPVDWDGDERSHSSRRWPHQGPGSSSSRYKQALLPTPEYGGGSSRSGFRHHSSSSSNPNYRAPFPSKRSMTYTNPNILKNRAAAKASEAKAKEQAATTQIPDKPIEAPKTVESANEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGG3</accession>
    <name>T1GGG3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin-protein ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02027324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027326">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGG3"/>
    <dbReference type="STRING" id="36166.T1GGG3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002483-RA">
        <property type="protein sequence ID" value="MESCA002483-PA"/>
        <property type="gene ID" value="MESCA002483"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_1_1"/>
    <dbReference type="OMA" id="HWTFVYA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14391">
        <property type="entry name" value="UBA_II_E2_UBCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23800">
        <property type="entry name" value="UBCc_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000010">
        <property type="entry name" value="Putative ubiquitin-conjugating enzyme e2 k"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000021">
        <property type="entry name" value="Putative ubiquitin-conjugating enzyme e2 k"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042614">
        <property type="entry name" value="UBCD4_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="11">
        <location>
            <begin position="4"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="161"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="199" mass="22405" checksum="DA320FF6D95420FE" modified="2013-10-16" version="1">MANIAISRIKREFKEVIKSEEIVQCSIKVELINDNWTELRGEIAGPPDTPYEGGKFILEIKVPETYPFNPPKVRFITRIWHPNISSVTGAICLDILKDNWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVATQYKENHPMFMLTARHWTSAYAGGPNKVSDFDAKIQRLKDMGIDENQARACLSKENWDLEKATESLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGS9</accession>
    <name>T1GGS9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02197287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGS9"/>
    <dbReference type="STRING" id="36166.T1GGS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002610-RA">
        <property type="protein sequence ID" value="MESCA002610-PA"/>
        <property type="gene ID" value="MESCA002610"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1039332_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="149"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002610-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="31419" checksum="DE81922B1500486B" modified="2013-10-16" version="1">MDFRIDFFDNPSKTYYPGEFINGCLLISVKRGEFIKNLLINIRGGVKVNYFDDVFHENFFDDKLLVIKDTLLEVGQHTFPFQTRIPEDCPPSLKSNHAYIKYKAVVVIPGVCNNTKIPFEFFVLSWVPMPFYEANVTSTANPHWPINKLAFPKNAFGPGEYINVIINVEEASAKTVKFYLMKYFWYKRNENCWGKQYKRKEKKEIFKIPIVTSNFTTHESLLLPKQLPSTLENFKFIEIGYILKIVIRNQTFKFPVVIGDYYKSDLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGW0</accession>
    <name>T1GGW0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GGW0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002641-RA">
        <property type="protein sequence ID" value="MESCA002641-PA"/>
        <property type="gene ID" value="MESCA002641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001238_1_0_1"/>
    <dbReference type="OMA" id="PQTYSIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000641">
        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000007">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044978">
        <property type="entry name" value="GRV2/DNAJC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045802">
        <property type="entry name" value="GRV2/DNAJC13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19432">
        <property type="entry name" value="RME-8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="1060"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1376"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1381"/>
            <end position="1390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1813" mass="207844" checksum="4FDBB405A05E79FD" modified="2013-10-16" version="1">MASGGFSRLHLFKAVNHLEIVQNISQRAAQYLGIEIKILKSQITLSQFERQRFGKYSEDQYQTSVTEFSVQKVTPRHPEPVKRILCLTESTILERDPQTYSVCTLRPLVDIFALVRDKDNIQMFCIEYKNGLVRSYITNDRDSLLATVLDAVRSSGNRDVHVRIANTPRGKRFVPLMNSVDEETEASLLRLIINNFQNQTRRFEILEKFNANIPHTGLKYSVTQDEKLIINALQALAQKEADNPTAQLSNLELEATFHAMSRLLASKVGYAAFTNMPGFREIIGSKIIASLRRNDLAVTYAAIDVINSLMHSMNSDHDLKQEQLNKSSLLSSKSFLEALLNMWTTHVTHGSGALVLSAMLDFLTFALCVPYSETTDGKQFDILLELVADRGRFLYKLGGEVEVAQQMQKLALDEAALCRHLLVALYTNPNEPTMATHRQLSRHLIGLWVTDNEEALELFRRIFPAGLLMFLESSDEVPIDHVEDDKLNFRDNLKFAVQHSNKTRKNVIEKHLEGIKHWGLSLIDHQEIVNQAIKNRPVVLRNRRQRKKTSDAVVNLPFFFYNFYKDHSLPNLIWNHKTREELKMSLENELRQFMNDRELAANLVVAWNYEEFEVQYHCLADEIKIGDYYIRLILENDNWPQNLVKDPIELFNALYRKVLCRNRVNDDQLTVTSLQALAKVYKRYHKEIGKFGDMAYIMQLIDRSNCRPLVDHVQTLVDLITLAHLHTGRAMPNTKTNVIEAGPNMEQYEEKDWYYNIEKEGSKPERQGPITYSQLKELWANGVINPKTRFWASGMDGWRSLQQIPQFKWCLMAKGQPLYNETELAQLILDILIKCTSFFPSRTRNGAAVLVPAPKMSRKLSEFSLLPHIVQVCLTHDPALLERVAILLCHIMEDNPEMSKVYITGVFFFMLMYTGSNILPIAKFLKMTHMKQGFRNEETSESGIMHRSILGQLLPEAMVCFLENYSAEKFAEIFLGEFDTPEVIWNSEMRRLLIEKISAHIADFTPRLKGHTMARYPYIAIPALKFPNWQIAEPVQLLKHTLNAWRKEVEKKPPQMTVKQAYEDLDIVIDENGPRPDESLIRKSYYKLAQKYHPDKNPNGREIFEKVNQAYEFLCSRTVLTGGGPDPQNIVLILRTQSILFDRYAEELRPYKYAGYPQLIKTIRLETKDDQLFSKEVPLLTAASELCYHTVHCSALNAEELRREEGIEALLEAYTRCVSILGADSKPDSLHYQVISNVTRCFEVACNFEKCKEKIITLPQLLSDVCRVVYFKHTLSISLVTNLAANNYNLQCDLYDYTLDESGVDADEKTNSQKVANNLAKMAILACISLAGYQMDLISPDEENGKQKIEKPPPPIPTKPQVKTSYTDNAQNLIQNSRQLTNSTVTNNSSKSPEKEVQNIEESSIVRKENLVNKQWFKVSGQANNVIVKKVLDKLLTIFISNKLSSEHENEILKLLNSNTRNPYYIWDNGTRAQLLDFLEQSRNQCSKETYEDISLIYEEVEGFVYDAHKEELQIGGVFIRIYNEMPTFPIVQPKQFTSELIEYLNQSYHFLTAPKVQSTGILQPSLAKGHPQYNDSNRQPSIQNNKTIDEVLNAYNRSKIRQVNTENHILMVLRALISVIKSNAECEKQCIGHFNTIFGFLTHGYFNQKPQIKALALEVVALVSRHKECVTEIAACEILGRFLIALRDPDMRDDQPRVLETLSGLMNAQEMIKEAQNKGAVIYLLDMFCNSRNPQIRETCADILSKMTADRLSGPKVRITVQKFLPPLFVDAMIESPATSVQLFESVHEHPELIWNDKTRSKVCDAVSEMCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH12</accession>
    <name>T1GH12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WIF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02177629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02177630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH12"/>
    <dbReference type="STRING" id="36166.T1GH12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002694-RA">
        <property type="protein sequence ID" value="MESCA002694-PA"/>
        <property type="gene ID" value="MESCA002694"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565004_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="8795" checksum="3523DBE5CB634B8A" modified="2013-10-16" version="1">MQFTVPVQADVYDLEFSWQSLTTHPVRLYPAPNQSPAPAKAPSQGPALLGAAACALGLVMIVGLIASAMYVRARKQIRQDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH30</accession>
    <name>T1GH30_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195357">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH30"/>
    <dbReference type="STRING" id="36166.T1GH30"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002713-RA">
        <property type="protein sequence ID" value="MESCA002713-PA"/>
        <property type="gene ID" value="MESCA002713"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_3_0_1"/>
    <dbReference type="OMA" id="YSYETDN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF237">
        <property type="entry name" value="CUTICULAR PROTEIN 65AU, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002713-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12032" checksum="64819FF696D4B101" modified="2013-10-16" version="1">VLACFVAVAFADAEVHEVSRSLDVGPDSYSYDVKLDNGVSAQESGKLKNEDTIAAQGSYSFTSPEGEKVEISYIADENGYQPQGALLPTPPPIPIAIAKSLEYLAAHPPKDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH60</accession>
    <name>T1GH60_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02200076">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH60"/>
    <dbReference type="STRING" id="36166.T1GH60"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002746-RA">
        <property type="protein sequence ID" value="MESCA002746-PA"/>
        <property type="gene ID" value="MESCA002746"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030703_1_1_1"/>
    <dbReference type="OMA" id="KTVHIHT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21432:SF20">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21432">
        <property type="entry name" value="ACETYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="3">
        <location>
            <begin position="40"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="4">
        <location>
            <begin position="304"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002746-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="457" mass="50061" checksum="8B736E43E93975A0" modified="2013-10-16" version="1">MYLSKFNRLSRLLVSPFHKTFFSYTNELSHPIPDRCPEIVATAEEAVSVIKSCDTVFCSGAAATPLVLIEAMVKHAKKNDLNGISVFHMHTEGEALYTKDEYKKHFRSGSLFMGGNVRKSVAAGESDNYSIFLQDIPKVFYKKIVEPDVSLVHVSAIDQHGYCSTGTSVDCVIAALKHSKKIVAQLNKQMPRTFGDSIIHSSHFDYAIEIDSPMPSHGGKPPNEIETKIGQLIAENLIEDGATLQMGIGNIPDAVLTALHDHKNLGIHSEMFANGVVDLVEKGCVTNSEKTFHKGRIVGSFFIGDQKLYDFVNNNPFIEMLENPKTTAINSCIEVDLTGQVNSDSIGTTFFSGFGGQVDFIRGAAECFDGKGKPIIAMPSVTAKGVSKITPILKPGAGVVTTRAHVHYVVTEQGIAFLFGKNMRQRAYELINIAHPDHREALEKAAFERLKVCPSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIN9</accession>
    <name>T1GIN9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIN9"/>
    <dbReference type="STRING" id="36166.T1GIN9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003322-RA">
        <property type="protein sequence ID" value="MESCA003322-PA"/>
        <property type="gene ID" value="MESCA003322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1039332_0_0_1"/>
    <dbReference type="OMA" id="NERRQIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF167">
        <property type="entry name" value="ARRESTIN C-TERMINAL-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="152"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="31273" checksum="DD397F9B292F1417" modified="2013-10-16" version="1">MLCDIEFINNPQKLYYTGGKVNVRVTLKLEVEKTIEELLAKVIGLAKVDFRRTSDKTHFMNQKNFLVVNQTLPPGTHVYEFEVEIPAECPSSFIGKYGKILYKVDVIASRLMNLNRKFSAEFHVIRPLTNFSVSNLPQKDEYFTYFCCWPCERKILLVEAMIPKSAFATGDCIPIFIKIDNCLENERRQINIRLMQLISYRGHRLDHETEKNVIQNITHVTSNYEVHEKMDIPHHIATSSDSCDILKISYSVNIFMDRIKFKFPVTIGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIY6</accession>
    <name>T1GIY6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003423-RA">
        <property type="protein sequence ID" value="MESCA003423-PA"/>
        <property type="gene ID" value="MESCA003423"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963428_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003423-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6832" checksum="833E9176AB8D7BBB" modified="2013-10-16" version="1">MEIKTEPEESFGTEFPEEEIKSECMDIEEAKSSPENSNKFCYLTNELPNIFARYSSSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJG8</accession>
    <name>T1GJG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02079557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJG8"/>
    <dbReference type="STRING" id="36166.T1GJG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003614-RA">
        <property type="protein sequence ID" value="MESCA003614-PA"/>
        <property type="gene ID" value="MESCA003614"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1951233_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="2">
        <location>
            <begin position="9"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="2">
        <location>
            <begin position="69"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14522" checksum="3A1AFA8D5BE8804A" modified="2013-10-16" version="1">MTNNAENYVVVDVDCGVDDAWSLLMLIRAEMKFKKFKILAITCVSGNTDVDNYLKIICSKMTNNAENYVVVDVDCGVDDAWSLLMLIRAEMEFKKFKILAITCVSGNTDVDNVARNTLRLLESLNRTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJG9</accession>
    <name>T1GJG9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02079556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02079557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJG9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003615-RA">
        <property type="protein sequence ID" value="MESCA003615-PA"/>
        <property type="gene ID" value="MESCA003615"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815365_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003615-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7722" checksum="BD0C675F7FD8B8E4" modified="2013-10-16" version="1">MCGLYDDNTICSAYLYKNCGSENYVMSIHLQTWNQQNTPHAMFLTFPNIDSNHSIGYGNWDPENIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK05</accession>
    <name>T1GK05_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP citrate synthase</fullName>
            <ecNumber evidence="2">2.3.3.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02159567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK05"/>
    <dbReference type="STRING" id="36166.T1GK05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003815-RA">
        <property type="protein sequence ID" value="MESCA003815-PA"/>
        <property type="gene ID" value="MESCA003815"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060828_0_0_1"/>
    <dbReference type="OMA" id="HMLRYQA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06100">
        <property type="entry name" value="CCL_ACL-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118:SF42">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003815-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="408" mass="44934" checksum="691DC92087AEF6E0" modified="2013-10-16" version="1">MSNELNNIVSKATDGVIEGVAIGGDRYPGTTFMDHIMRYQADPEVKMIVLLGEVGGTEEYEVCQALKDKSITKPLVAWCIGTCAGMFTAEVQFGHAGSCANSDRETATAKNRELKVAGAYVPESFDTLGDLIGQVYKELVKSGRIVPKEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELLYAGMPISDVLNKNVGIGGVISLLWFQRCLPPYVCKFFEMCLMVTADHGPAVSGAHNTIVCARAGKDLVSSVVSGLLTIFVNAMRKKGQLIMGIGHRVKSINNPDVRVKIIKEFVLENFPSCPLLNYALEVEKITTSKKPNLILNVDGVIATCFVDMLRNCGSFTNEEAQEYINIGAINSLFVLGRSIGFIGHYMDQKRLKQGLYRHPWDDISYVIPEQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK55</accession>
    <name>T1GK55_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vinculin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02143559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02143561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK55"/>
    <dbReference type="STRING" id="36166.T1GK55"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003871-RA">
        <property type="protein sequence ID" value="MESCA003871-PA"/>
        <property type="gene ID" value="MESCA003871"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015314_2_0_1"/>
    <dbReference type="OMA" id="MNNMRQF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001033">
        <property type="entry name" value="Alpha_catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914">
        <property type="entry name" value="ALPHA CATENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914:SF9">
        <property type="entry name" value="CATENIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00805">
        <property type="entry name" value="ALPHACATENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003871-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="860" mass="96552" checksum="074BE198169877F2" modified="2013-10-16" version="1">MTWGFNLSHKIDARILKGILRRTVVISVPEAININNENVESASKLCSFGHPACIFSSPIFPFEFVAIHVLLFLCAQREVLPFEERKTLVGKSKRASALVGAVETATENFIQKGEQIAYENPEITQEMLVTVNEVRKSGNEMSVASREFSEDPCSSVKRTNMVRAARNFLSTVTRLLILADMVDVHVLLKSLNIVQSDLDKVKNALSQDELLNNMRDFDRNANELIKQAAQRQAELKDPQLRDDLAAARAVLKRHSTILLTASKVYVPRVNRDYVLKQVCEAVNTISDITQGKAPKRVDMDMYGGTGELAAALDDFDGSVVVKPATYSDTETRKQLEERLESIISAAALMADADCTRDERRDRIVTECNAVRQALQDLLNKYVVHSGPRGNKEEIKIAIEMMRKKTCDLRRQLRKAIVDHVSDSFMEVTTPLLDLINAAQSGNRRAVHEKVDVFTKHAEKLIEVANLVCSMSTNEDGVKMVRYASAQIDLLCPQVINSALILTERPSSRFAQENMDSYRFTWENQVRILTEAVDDITTIDDFMAVSENHILEDVNKCVMALQEPCPMTLHTTASSIQGRSLRVCDVVAAEMDNYEPCPYTKRVQMLFFAERVNVAVGILSNPSGHKDIDENDFIDASRMVYDGIREIRRAVLMNRNPEDLDTDTEFEAGEDAFDNRSRSSVQTAEQTIDEYPDISGIYTAREAMCKMTDEDKQKIEKQVEAFKREKLTFDSEVAKWDDAGNDIIFLAKHMCMIMMEMTDFTRGRGPLKTTMDVINAAKKISEAGTKLDKLTRQIADQCPESSTKQDLLAFLERIALYCHQIQITSKVKADVQNFSGELIVNGLESATSLIQAAKNLMNAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKR3</accession>
    <name>T1GKR3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02067750">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02067751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKR3"/>
    <dbReference type="STRING" id="36166.T1GKR3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004090-RA">
        <property type="protein sequence ID" value="MESCA004090-PA"/>
        <property type="gene ID" value="MESCA004090"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_284785_0_0_1"/>
    <dbReference type="OMA" id="THEEQMI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="696"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="778"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="787"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="839"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="893"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="945"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="600"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="603"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004090-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1090" mass="117862" checksum="202C85908E1D2A39" modified="2013-10-16" version="1">MGKCTHGEIMIMVGFERQQGSGNTQVNKKPSLVVGLDKVFVNRVACGSSHSIAWGLPQSPIEEGKKGPVPFLTTKDPLGGNSLGIYDSEVTPNISTPISKQSSKQPSLSEIIMSLETLGARQTALNYILNAMSIIQARHLIVSSLTSHSKGGGEAPADNTDPVIQEPSPTEIGQSATMSAAMSSSAGPLSAFQSLTGSLSLSSVSSCGGTNPNQKLSKMSTSAMSVIMAATMTHEEQMIEAAHNNGLDDFTALLTESEAKSLIELLKLNVAGRFGLGSQYTNSWQTISDTLIALASNSSSISSMLIETCITELEDLCTSRQCLFGKMPKSVIQETSHPYVDDVTLTGHVRIPGAEALRLEFDTNCSTEKRNDPLVIFDGAGRIVATRSGRELAQWTPEIRIPGDEMRWKFTSDSSVNGWGWKFWIRPFTTFYSLVMALLDSKKINPGQSTVLIRLASALAQCSQLNSLTPTQRIWSLKKLHSILNSKNAPKHSEPVIVSILTPLIPEIYHQYEYEEPQVRGGMHLMHSEYFKTLVALACDMQLDSLIPQSDLHKWSWFRRYCTAVRVAQSLVHRVELPQQFCVEVRKKISEMLPSPSNVTIGTSMTTSSTSLSSSSSNNTPQPVTPSNFLTAHNLNRSCSNTDVQDLTEQCSNISQSQFVYIHEDHNLFKSEHDSQLLQWLNRRPEDWALSWGGASTIYGWGHNHRGQLGGLEGSRIKSPTPCEALSLLRPVQLAGGEQTLYAVTPDGKLFATGYGAGGRLGIGGTDSVATPTWWRGKHCLALTTDGEVFSWGEGEDGKLGHGNRMSYDRPKLIESLSGIGIVDIACGSAHSACITSSGQVLTWGKGRYGRLGHGDSEDQLRPKPVEALLCYRAVDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPMKIESLSGLGVTKVECGSQFSVALTKSGAVYTFGKGDYHRLGHGNTDHVRRPKKIAALQGKKIISISTGSLHCVACSDTGEVFTWGDNDEGQLGDGTVSAIQRPRLVTALQGKHIVKVTCGSAHTLALSTSQLSEAARPPPNPPIEYDLVGELSPDALHARLVLLHHFSELYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL35</accession>
    <name>T1GL35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390066">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004222-RA">
        <property type="protein sequence ID" value="MESCA004222-PA"/>
        <property type="gene ID" value="MESCA004222"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760693_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004222-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8008" checksum="A071E95C633083EF" modified="2013-10-16" version="1">MQLRDSTKHILTDFKPALLTNESKRIGQGKMIEEFSLFEMQQGWGLSVIYELLKIVPDSSISSAEMLVFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL72</accession>
    <name>T1GL72_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004265-RA">
        <property type="protein sequence ID" value="MESCA004265-PA"/>
        <property type="gene ID" value="MESCA004265"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1456024_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004265-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="20922" checksum="6EE13FBAF9BD2418" modified="2013-10-16" version="1">MEMRPTIMPIVMHQPQQVQRPAQAQIVLHHQPTHVQQQQQLQQQQSQQPQVTSNNIGIIKTQQVPATIIGRNIIIPQQQPQQQPQVTNQQQSQQKISNAAIINLLNSAPAAMTTNTLLNRSAGNITPQTQPNQQNAAQQVVQQLATASTNTSNDSLQQQQQRNRVFPALQIKVLTYLVFSNHLKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL85</accession>
    <name>T1GL85_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ZNF598/HEL2 PAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02161257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL85"/>
    <dbReference type="STRING" id="36166.T1GL85"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004280-RA">
        <property type="protein sequence ID" value="MESCA004280-PA"/>
        <property type="gene ID" value="MESCA004280"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_710923_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057634">
        <property type="entry name" value="PAH_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23202">
        <property type="entry name" value="PAH_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZNF598/HEL2 PAH" evidence="2">
        <location>
            <begin position="256"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004280-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="389" mass="42605" checksum="9FCD75FB33BD7FC6" modified="2013-10-16" version="1">DFEIPPEQPTRPQSIDARDEQQFPSLLSSNAANANLSFQPLDIRNKFGNAGLARTKENFPALGGGGSGAGPSNNGSKNSSKSQSVSNILKKTPNPTNSGLKEEVKPESKVTAPSLTKQDFPSLAKVSKSNSDFPVPKTPNGKKTTNTSSWANTTQLRKSLSKTNAKYGLDNNNKKDVASKMNGNTKQKDDKKDKKGKNSNATPPGFDGPKPPPGFSSVSLNSIAKTANNMTFTSSMGDKFRIVPSFQFTPPENCGKRNQDLVKIFKSSMPKAEAMEDFRNNTQRFRNGTFNAGAYYEHCESVLKDKFHVVFPELLALLPDIGKQQELYLVYSQRLKQLQNKGITNKLKVCSVCKQVLLTTDYIVHMAIQKTTTKPNKTLVLNYFVALGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLC2</accession>
    <name>T1GLC2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02197377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004322-RA">
        <property type="protein sequence ID" value="MESCA004322-PA"/>
        <property type="gene ID" value="MESCA004322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852283_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7044" checksum="3DFA755DF99A1B36" modified="2013-10-16" version="1">MKSATSKQILGYADDIDAIGKTPSEIIDKFLTIEKAANSVGFNVNGILQTDEIGAKPLRCLLKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLT3</accession>
    <name>T1GLT3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLT3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004497-RA">
        <property type="protein sequence ID" value="MESCA004497-PA"/>
        <property type="gene ID" value="MESCA004497"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3377606_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004497-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="34" mass="4198" checksum="7806DCC3FDE83460" modified="2013-10-16" version="1">MPDNRSTQREKHHQFTQILEMMNTGRREIEITRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLY0</accession>
    <name>T1GLY0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Skp1-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLY0"/>
    <dbReference type="STRING" id="36166.T1GLY0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004546-RA">
        <property type="protein sequence ID" value="MESCA004546-PA"/>
        <property type="gene ID" value="MESCA004546"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059252_4_0_1"/>
    <dbReference type="OMA" id="DKYTASM"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000018">
        <property type="entry name" value="S-phase kinase-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="3"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="112"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004546-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18375" checksum="83D105338EC96A48" modified="2013-10-16" version="1">MPNIKLQSSDGEIFDTALDIALCSLTIKTMIDDCGIDEGEDTVVPLPNVNSTILRKVLTWAQYHKDDPMPSEDDDSKEKRTDDISSWDADFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTPAEEEQVRKENEWCEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM66</accession>
    <name>T1GM66_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02090055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02090057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004634-RA">
        <property type="protein sequence ID" value="MESCA004634-PA"/>
        <property type="gene ID" value="MESCA004634"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457353_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004634-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10105" checksum="B317527FB922A890" modified="2013-10-16" version="1">MIHRTENGTSGNTSGVKSVIRRRRKNRVLRIAVRASSVVGIGASSFEGIRSFLRGYFKYFTEELPRGEHKISQARITKATSSRKIYNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GML1</accession>
    <name>T1GML1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GML1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004787-RA">
        <property type="protein sequence ID" value="MESCA004787-PA"/>
        <property type="gene ID" value="MESCA004787"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2925242_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004787-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6583" checksum="1378A47B6F172E20" modified="2013-10-16" version="1">MSSLLTLELISPNAGMKVDPASLSTGSGDSRNCEKHSFSSLQCKEEDGYRRIHSNKIKGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GML3</accession>
    <name>T1GML3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02038890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GML3"/>
    <dbReference type="STRING" id="36166.T1GML3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004789-RA">
        <property type="protein sequence ID" value="MESCA004789-PA"/>
        <property type="gene ID" value="MESCA004789"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1210995_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000103">
        <property type="entry name" value="Troponin C Ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="162"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="198"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="229" mass="25954" checksum="1FA22A8CE3ACC52C" modified="2013-10-16" version="1">MLKDNLNSQFKNTVLLGVESVLLPPFKKSKRESKINKRLMSDRIIDLVIIAKIAADLESSSKLLSTQQMLFPDRRARQRTTKKNFSAVLRNAFKAFDADNKGFIETADFHTILEMMGQKLDDKAEKQLVKDNKKGSPPGKINFDEFCAMAGRFVEVEEDAAAMATELKEAFRLYDREGKGYLTVKTLRDILHELDDKLSSQELDMIIDEIDADGSGTVDFDEFMTIMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMM6</accession>
    <name>T1GMM6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMM6"/>
    <dbReference type="STRING" id="36166.T1GMM6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004802-RA">
        <property type="protein sequence ID" value="MESCA004802-PA"/>
        <property type="gene ID" value="MESCA004802"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009108_0_0_1"/>
    <dbReference type="OMA" id="INCIISH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000498">
        <property type="entry name" value="Striatin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000058">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="351"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004802-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="429" mass="47132" checksum="521326DA24B1D8BA" modified="2013-10-16" version="1">PLANFEFLGSNEISDDDDISDDFDLGSGDDADADLKTSKRIKAKDVSDDGISNSDVLRRNVTSNEDEIVDSSGLGLGELAQLTVNNESDLNYDGNNAKDAIYRKTWNAKYTLRSHFDGVRALAFHPEESVLITGSEDNTLKLWNLQKTVPAKKSTSLDVEPLYTFRAHNGPVLCLSMSTSGEFCYSGGLDGDINCWKLPNSNIVRHTDAVWGLCILNNQIKGDYLASCSADSTVKLWSPNVLSSDTSKMLPFLNSYSSEVGGIPSSVDFVRNELDRIVVAYNSRSTHCVIYDTESGKEIVKLEATHEMSGNTGKFINKVISHPTLPISITAHEDRHIRFWDNTTGSLIHSMVAHLEPVTSLAVDAHGLYLLSGSHDCSIRLWNLESKTCVQEITAHRKKFDESIFDVAFHPSKPYIASAGADGLSKVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GN80</accession>
    <name>T1GN80_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02025432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GN80"/>
    <dbReference type="STRING" id="36166.T1GN80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005024-RA">
        <property type="protein sequence ID" value="MESCA005024-PA"/>
        <property type="gene ID" value="MESCA005024"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027239_1_1_1"/>
    <dbReference type="OMA" id="HEENILF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005024-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="23262" checksum="CADA8FB9393EBDF8" modified="2013-10-16" version="1">MLPYEIWTKKPKIIYITRNVKDSILSRYHMYKGFNYWKGDMKPFLDAFMNDDLAYLPYWPHILEFWQIREQPNVYFTSYENLKKDLKGSLRKICKFLERPFTEELLDKAVFHLSFENMKNSKGHQEGEEMIKQFRKAVGLENNDFEFLRKGKVGSFKEELPEGYAEKLDEWSAGFLEKAGLTMDDILNYEKEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNQ2</accession>
    <name>T1GNQ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Myelin transcription factor 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNQ2"/>
    <dbReference type="STRING" id="36166.T1GNQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005210-RA">
        <property type="protein sequence ID" value="MESCA005210-PA"/>
        <property type="gene ID" value="MESCA005210"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002921_0_0_1"/>
    <dbReference type="OMA" id="GNASQMM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000001">
        <property type="entry name" value="Myelin transcription factor 1-like, a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF15">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="74"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="424"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="503"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="647"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="168"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="236"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="453"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="711"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="723" mass="77071" checksum="552B9B27CCAFEEF5" modified="2013-10-16" version="1">MHEQIIKCPTPGCNGRGHISSHRTTHRSLSGCPTAAASKVAGRELKLGYSKTANQLSPLQKQPEIKNVFTIKDDKDQPQQVHKSPYLNNNNNNNANCVTTTIPTARSEYSSSNSKSPVPPHNNNNNSNNKFDPYSTNTDSTNPSNSYTNIDSQSNNGSRHSTPGGGNSINSNPNNDGSSIVHSSHLTNRTSNSTTSSSSSTNSSTDMNGYSQQQQQQQGPQQQQETTAATRGTYEGSNGNLMMNSNGVSQGSSGDGSSTTAPSSDPHMIIETDAYGREHMRYSHNIPQMNNDINGRPTYDVTVPATAFERYDPNVCGNQRSPVVSSSLSSNMYPYLTQSAAAVGVTMDEITSQQQKYLHEQQMVHASMLKGEHVDELNGGPLYPRPMYHYDPSMGPLPPGFSAINLSVKVAAAQAAAAAAAFNNNNNNSNTGQPKSGSVSPPPQPPNGPIIDLSTSTILNSSPQPGASPNLASPQVPSPQGQTLDLSVTRLQHARYLIASPQYGAHPEGLTHPHGFASTPRSPQTEPVDFSGPPRPLGFGLVGHLGAHGPYSRESTPDSGASHYIDSYRDPSDFGYNIPTGYSPHPSYGMVVQSDYPPTGYHGYAPTAYQCTNPYATAVGPAAYPTPVSGGYSPSAASCYSMPPPQHIPQLDKTKDGLTGMPRTDRNHLQSHSQELKCPTPGCDGSGHVTGNYSSHRSLSGCPRANKPKSKPRDGQDSEPLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP57</accession>
    <name>T1GP57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidine-rich glycoprotein-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP57"/>
    <dbReference type="STRING" id="36166.T1GP57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005374-RA">
        <property type="protein sequence ID" value="MESCA005374-PA"/>
        <property type="gene ID" value="MESCA005374"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1499974_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577060" description="Histidine-rich glycoprotein-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005374-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20371" checksum="F3F45A4F0080298D" modified="2013-10-16" version="1">MKTVLGILLTLVYVADSTFFGSKKSEHIVHHIEVPLHFAEPHHEHHEVKVVEHHHHHHHKEHHIPVHHEVHHEEHHEEYHAPVPIVHHEEHHEEIGHHEEKHDFGHDFGSEGHDFGSESHSFGSEGHDFGSKSHSFGSEGHDFGSESHSFGSEGHSFGSEGHDFGSDSHSFGSEGHDFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPA3</accession>
    <name>T1GPA3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02113628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113632">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02113633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPA3"/>
    <dbReference type="STRING" id="36166.T1GPA3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005428-RA">
        <property type="protein sequence ID" value="MESCA005428-PA"/>
        <property type="gene ID" value="MESCA005428"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1074675_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005428-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="29978" checksum="6F13FE94FBCF2794" modified="2013-10-16" version="1">MVPSRPISKSNKETGAGESHGSESNSSRNWFKNRRQRDRAAAAKNRLQQNQNAGSSGRRGDGRLSPTPSEDSDSDISLGTHSPTPNPLLLQHSPESISNGGHDREDTLTSEDEKSKEYTPSFMTAHQHHIMPPSLQMPHFKIDSAATANLLSGFYLPNFSAAAAAARGGLGQFPIQPTVIRPHPQHLPPTAVAGQQPIVHLNLSATAVDSSDSNCVATNLSNSSSSAHSPYEDISGASSPPVVHPHLHHPTTAHHHALQFSSLHRPFSTTPEPNVTPDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPL4</accession>
    <name>T1GPL4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02022883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02022887">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPL4"/>
    <dbReference type="STRING" id="36166.T1GPL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005548-RA">
        <property type="protein sequence ID" value="MESCA005548-PA"/>
        <property type="gene ID" value="MESCA005548"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_408044_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005548-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="674" mass="73268" checksum="BE463131AC6C981B" modified="2013-10-16" version="1">RSRVLISKLTPLWLPQQIIFSLALAGGNSVENLSALCDILGISRHPPLNPFETKPVTIQVRSIPLDLLGWGMAIKSNSCTLKNLEVIYQMEKHNNLETRNAVDVFLDSLGQNESISTLRFSSLIEVDVKDTEINYLANCVSKVLLKPRLENFELVLTLLEEDPPILKLQPVITAICRAITKQSKLSFLLLDLGLSTSQGMYAPTTTLGRSATLPRQPIEPTIDKRSCDSVVSKTWYPTPACEGGPHSSGTLHDLLLAAREPYSKLNGLDLSKAQLSLEDSMCLGETVRISNTLHTLKLEGCSRLSEILPSIIGASESMCIQMLSFGSPRLNLEDSAISMCSRALTSCSTLRLLSLDGWSFRIEELLPKGPLNPFSSGFGGFAAAWNSLESLGPKLLIILDGYDTQKSSPKRKGKHYPSDVHDLLDGLTWGRSASILLGGGQWGGPSRLLDTVQECPHLRNIVRTPLGCLAVSALFESISSSGDGDLPSEELDVIESILHCVTGDPLPQHIAELGRLAMFCLKMKRPSITLAELKMYCSSPESTMMGCLDRNLLVGKAAKKKGEFVYNFICPNMAEYLAASYLASLANRPGLLAAEITGLAIGGELDMDLLKVIQFAMGLLGPRSHVLISKLTPLWLPQQIIFSLALAGGNSVENLSALCDILGISRHPPLNPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ43</accession>
    <name>T1GQ43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TMC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02064822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02064827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005743-RA">
        <property type="protein sequence ID" value="MESCA005743-PA"/>
        <property type="gene ID" value="MESCA005743"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013958_1_0_1"/>
    <dbReference type="OMA" id="HEVEGRF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF24">
        <property type="entry name" value="TMC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="584"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="507"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="654"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="666"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005743-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="681" mass="79897" checksum="FE32F2B99C287AF1" modified="2013-10-16" version="1">MGRSWRRILPRNFPGKETSEWEGLQKDSAAIATLLPSKQNRAATTRRVRPVQQDTKNTRKRQQQSTARRRNSTVQRRGSQYQEVQVAMLPDLSQDLTDEERIWEEIHEIKTMPVSMAQKKEMKAQLQNATKLRLQGLDQFKWQRRKAWQQLEMKWSEILAKTELWRNSLKKIEGNFGTGVVAYFLFLRWLMVLNLVIFSLILIFVVIPQIVYVSKSTSWPKYEDSPNVPGTGFMEKTLMFYGMYSNKIFGYIEQPSSNFTESIDTNSTSESFSKKYYYDLPLAYVSITAVYFLLSLAAIVKSASKEFKDRLVDGEGQFYQYCNLIFGGWDFCIHNEKSADIKHKALYNEIRGLLHTKQIEYERCNRSKDYMCKLIVIRFFINIAVLIILAMAAAFIYFFFNISLQHLEGKILIQESSDYSEKLKILFFEFLPYLAIVCLNLLVPILFNYLVEFEKYSPMFVIKISLFRTVFLRLSSLAVLLSRFYFLIIPNKDDVQCYEKQASKPQCWETYIGQQFYKLVITDFGSHIFVTFFVNFPDPSVIPVVYSIAEISPSRSCGPFRGRLNVWEAAIEAFEKMPSLFRSLIFFLGTAGFAIPCFIVLALFLYYYYAVSDANKHMVEVLKNQLVLEGHDKQFLLHRLSTFIKQQQEFQKKMRHFDQQQQQQHNVRENNKSNRDDRHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQQ1</accession>
    <name>T1GQQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02190841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQQ1"/>
    <dbReference type="STRING" id="36166.T1GQQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005962-RA">
        <property type="protein sequence ID" value="MESCA005962-PA"/>
        <property type="gene ID" value="MESCA005962"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_7_39_1"/>
    <dbReference type="OMA" id="ASMSHAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05040">
        <property type="entry name" value="PTKc_Ack_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09539">
        <property type="entry name" value="SAM_TNK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000080">
        <property type="entry name" value="Putative activated CDC42 kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000107">
        <property type="entry name" value="Putative activated CDC42 kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.680.10">
        <property type="entry name" value="Cdc42-like binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055175">
        <property type="entry name" value="ACK/TNK-like_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015116">
        <property type="entry name" value="Cdc42-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037085">
        <property type="entry name" value="Cdc42-bd-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049587">
        <property type="entry name" value="TNK-like_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09027">
        <property type="entry name" value="GTPase_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22931">
        <property type="entry name" value="SAM_TNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="102"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="474"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005962-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="909" mass="101787" checksum="0D3812186ED3FB6E" modified="2013-10-16" version="1">MVGRSSPGSSIRQFLSAIRDDLQVTRLAHFDYVHANDLERCGLGKPAVRRLLEAVRKKKSHQWRKNILSKLIGGGKQPNKKQANHQIDPIVGTSHLIQEKDVILGQKLGDGSFGVVRQGEWNAPNSRKIAVAVKVLKADSLTQPGILDDFVREVQAMHALDHPNLVRLFGVVLTTPMMMVTELAEKGSLLDTLRKQCKNTPLPTIWNWSVQIATGMAFLESKRFLHRDLACRNVLLAAGNKIKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLRYRQFSHASDTWMFGVTLWEMFTFGEDPWVGLNGSQILKKIDREGERLHHPEACPPDVYELMLQCWDKTPSERPILLPLSEQFLASGAPQLQRAIMSYSEPNRLKINQNDQIAIIDGRPELNLIKGQNQSTFDIGIFPRNILDKQKNGDISRPIHDSLRHTGHGSAFGFSWGSPAFIDEQSGNDIVQMRNSGEISCLNRQKKSASMSHAKERKSIASKQFSYNKLKSDLAGQKTQKSTTTPSKMKPNRPPQPQMQTEGILIDISPDDASSLAPPPSQIDSLSLQISNSFCILDAPIDVPTQEEEEPRVPPPYQMPPTYLNTFDITNSPSNSNMGYPPPENIYANSQNNSIFDSPQNNGIYANTQNGAIPKIPNGHMAKLDKSFIAELEKDIYKDSMTTNMNSSQICSNKEIVFKNDLSPLKKNGTLNNSLYEKNTNIELSPPQALVNKIWYETQAATYAPPPVQQVALPPQVPQQGPIDPMSIYQNSSTIQTNPQVNHTYVVKSNRPPPVYNYASDFYDAVAPTPSSYYGSVPTTSVIYDEVTSDDYLRPHRPAPQLSAQQIQRRLEKMKQQQQTSQQIYQYTQPQENTYDVADTGEHDRISAVLKELGPGAIEEDVKASLMACGWDHQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQX6</accession>
    <name>T1GQX6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Probably acts as a transcriptional regulator. Required for the specification of the tarsal segment. Also involved in antenna development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02111680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02111681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GQX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006042-RA">
        <property type="protein sequence ID" value="MESCA006042-PA"/>
        <property type="gene ID" value="MESCA006042"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027674_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007455">
        <property type="term" value="P:eye-antennal disc morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046660">
        <property type="term" value="P:female sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007478">
        <property type="term" value="P:leg disc morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000120">
        <property type="entry name" value="Bric a brac 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF109">
        <property type="entry name" value="FI07618P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="65"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006042-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="327" mass="36367" checksum="48CFA662C7461317" modified="2013-10-16" version="1">MSSESSPPPPDKSLSISPKPEAHVSPPPSDHLSANKHQQFCLRWNNYQSNLMSVFDQLLQNESFVDVTLACDSQSIKAHKIVLSACSPYFQALFIDNPCQHPILIMRDVKWEEMKSIVDFMYKGEINVSQEQIGPLLKIAEMLKIRGLAEVNSSETTIESEPDSELHLPEPVAAKKPRIDVVKWPSKSQSPSSSSSSSHHRLISSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPPVRPMSHPPLITTHHQQPPLAPPPSLPPLAAVHPHQHQQPPTPLDPMGLMGLAPHVDDLEIKPGIAEMIREEERVSLAFLFKDFNGVFWAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR23</accession>
    <name>T1GR23_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR23"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006094-RA">
        <property type="protein sequence ID" value="MESCA006094-PA"/>
        <property type="gene ID" value="MESCA006094"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2102978_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577092" evidence="2">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006094-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="116" mass="13356" checksum="2475723C0E400EB3" modified="2013-10-16" version="1">MNKSFLLLVVVCILLLENSKGYGQHMPPYSLQPALESLHRRDAIAGHVPSQSQIDYNDEDVNEPNPSRNSKIDRLIAAYLAKDYLEDDDSEDGNYFLPEEKKKSYFRERDEKVDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRF7</accession>
    <name>T1GRF7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02394808">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRF7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006240-RA">
        <property type="protein sequence ID" value="MESCA006240-PA"/>
        <property type="gene ID" value="MESCA006240"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827513_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006240-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16146" checksum="00E1236044540638" modified="2013-10-16" version="1">MREKFAPVRNFTLRVPFAHYFFPKKVTPQDEDSLSFDNTEDVPLPSLEKVGTLPTISGKVAHTSSTNVIRSIGSYQCGFMSGRGTSDHFFTLRQILKKPRNPSRHLHLFIYNQAYNTTFTLELFKAMNQFPISSLNSAKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRY4</accession>
    <name>T1GRY4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02019855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006431-RA">
        <property type="protein sequence ID" value="MESCA006431-PA"/>
        <property type="gene ID" value="MESCA006431"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2006526_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006431-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="124" mass="13863" checksum="6336A856D91062E1" modified="2013-10-16" version="1">MEVGQKYLEFQSRVFPVTGPATTTSGTRILEVPTTMFEEMSLSDEDGLPDEIYCLVISQGGEFIRLRIKLLTSSLSMYKGCPESSASKRSQENIILPPGKRIPYSDKARLRRGCKSPDLKMNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS13</accession>
    <name>T1GS13_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS13"/>
    <dbReference type="STRING" id="36166.T1GS13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006460-RA">
        <property type="protein sequence ID" value="MESCA006460-PA"/>
        <property type="gene ID" value="MESCA006460"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021447_0_1_1"/>
    <dbReference type="OMA" id="DQIEHRH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.420">
        <property type="entry name" value="AHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="5">
        <location>
            <begin position="35"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006460-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="353" mass="40665" checksum="1BD6D8685846AEA9" modified="2013-10-16" version="1">MSEGKPLQIIKSVKDSFELDEMVLSQILENDEIKDKFVAVISIAGPYRKGKSFLLSFFLRYLNAMYIKKDISNWLGNPKDPLQGFSWSNGMERNTTGVWIWSEIFIHEKTAIILLDTQGVFDSKTSSKNCAVIFGLSTLLSSVQIYNLSGNIQENDLHHLMLFTEYGKLASENSESTPFQNLLFLIRDWSYSYSFEYGPVGGHRKHLKNCFSKVECFLMPHPGMKILNNESFDGKLVDISEEFLEKISSLVNMILSPNRLLIKEINGEKIRGKEFLQYFETYFQVLKKNEVPEPKTIYLAIAEANHLNVLMSCKENYTSKIKNLFEESQFLSSEDFVIKHESIKLEILKMVCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSQ1</accession>
    <name>T1GSQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho GDP-dissociation inhibitor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02060936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSQ1"/>
    <dbReference type="STRING" id="36166.T1GSQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006712-RA">
        <property type="protein sequence ID" value="MESCA006712-PA"/>
        <property type="gene ID" value="MESCA006712"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076228_2_1_1"/>
    <dbReference type="OMA" id="ICNDAPK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000004">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006712-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15850" checksum="B34103651756FC33" modified="2013-10-16" version="1">MSDNNKVTNNDDSENESGDYVPPPQKSIEELLAADQEDESLRRYKETLLGNTSDKIIFDASNPKSVIVKKLSLLVDSRPEKELDLSDISSLKKQVITIKEGVNYKVKIDFVVQREIVHGLKYVQKTYRLGVPGNISLHYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSR1</accession>
    <name>T1GSR1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02131418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSR1"/>
    <dbReference type="STRING" id="36166.T1GSR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006722-RA">
        <property type="protein sequence ID" value="MESCA006722-PA"/>
        <property type="gene ID" value="MESCA006722"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1437845_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588479" description="Cathepsin propeptide inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="2">
        <location>
            <begin position="59"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006722-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="189" mass="20973" checksum="397704F2C1DA3F1D" modified="2013-10-16" version="1">MKLSLGFLLISSCFIASYAVPQFGFGLANRFADRVSNLPGVNRIAPLAQFPKLSSVRDFGEFVAQSGKKYASAAERTLREGIFAAKNSVLEANNAAFNAGKSGFTMAHNIFSDIEKSEFLQKLTGNKKSTTGEYVFKFMFKGKTPFSDIYIYFKKIESKNRKSTPKISASIPESFDWREKGAVTPVKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GST6</accession>
    <name>T1GST6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02152976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GST6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006747-RA">
        <property type="protein sequence ID" value="MESCA006747-PA"/>
        <property type="gene ID" value="MESCA006747"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1984111_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006747-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14201" checksum="D566B9AC98FE6BEE" modified="2013-10-16" version="1">MAELDCRIIAIFTLALSLVFLGSVIFFRHHQHIHRLTEDHFRDRVHRSSAEQLSDDDAGHEIKCLTKSDNPSSSSQPHIEESCFQCLCETMTDCKPAKCNDVPCGVPGSPNGLKYDRKNQCDIIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTN7</accession>
    <name>T1GTN7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTN7"/>
    <dbReference type="STRING" id="36166.T1GTN7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007075-RA">
        <property type="protein sequence ID" value="MESCA007075-PA"/>
        <property type="gene ID" value="MESCA007075"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313166_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007075-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11723" checksum="6DDB5CF39051951D" modified="2013-10-16" version="1">MDVSTWLRKAREMNIKDQDTQLNIHWYIPFYSLYYILTGLHLNFANSSFGVGRRFRRLNLALKRYYLSNELLEPWVKVSQIPTPAAAAPMTIKNLADMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUV3</accession>
    <name>T1GUV3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02137546">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUV3"/>
    <dbReference type="STRING" id="36166.T1GUV3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007524-RA">
        <property type="protein sequence ID" value="MESCA007524-PA"/>
        <property type="gene ID" value="MESCA007524"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_10_1"/>
    <dbReference type="OMA" id="NEANDMD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF32">
        <property type="entry name" value="PROTEIN WHITE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter family G" evidence="8">
        <location>
            <begin position="100"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007524-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18841" checksum="57526BE6DF984588" modified="2013-10-16" version="1">MWMASTLKISNKSMNMKERELLVENILDSLSLTKARLTKCCQLSGGQKKRLSIALELIDNPPILFLDEPTTGLDSSTCYDTCMLLNQIAKEGRTIVCTIHQPSSTIFEMFNHIYVVKNGYCVYQGTPKNTVPFLREIGMNCPEYHNPADYRKYFDLFLEPGKMNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVS7</accession>
    <name>T1GVS7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007893-RA">
        <property type="protein sequence ID" value="MESCA007893-PA"/>
        <property type="gene ID" value="MESCA007893"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504279_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007893-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9160" checksum="1B101DC9729203BE" modified="2013-10-16" version="1">QSKSIIFDNKACLVKDPATNSTLDLSKFEIVERKFESATGNISMSFNLCGKLAGGCGTELNSSACLSIGKKDFVLGLEGEYGFQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVZ8</accession>
    <name>T1GVZ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LanC-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02032285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVZ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007966-RA">
        <property type="protein sequence ID" value="MESCA007966-PA"/>
        <property type="gene ID" value="MESCA007966"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519029_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031179">
        <property type="term" value="P:peptide modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007822">
        <property type="entry name" value="LANC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020464">
        <property type="entry name" value="LanC-like_prot_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736">
        <property type="entry name" value="LANC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736:SF7">
        <property type="entry name" value="LANC-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05147">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01951">
        <property type="entry name" value="LANCEUKARYTE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158745">
        <property type="entry name" value="LanC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR607822-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007966-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9810" checksum="D29E8B5B3BC93BD3" modified="2013-10-16" version="1">MKSYIIFKDEKYLKSAELASDVIWKKGLLLKGPGICHGVAGNGYAFLILYRLTNNPKYFYRASKFMEFLTHPEFKAKANTPDRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW68</accession>
    <name>T1GW68_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051236">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051237">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GW68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008040-RA">
        <property type="protein sequence ID" value="MESCA008040-PA"/>
        <property type="gene ID" value="MESCA008040"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_505869_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.460.10">
        <property type="entry name" value="Inositol Polyphosphate 1-phosphatase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044897">
        <property type="entry name" value="INPP1_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF3">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 1-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008040-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="539" mass="59659" checksum="B4179606E7DEBADC" modified="2013-10-16" version="1">ATKEYINGDTVFSGFPGITSSGLDCVTVLIGVFDRNSGNPLIGVINQPFHKKIGTDSYERITSSGLDCVTVLIGVFDRNSGNPLIGVINQPFHKKIASDSYESKVFWGVSIEDFKANNISNLSNDSKIAIFSTSEESSVFEKLFQXXXXXXXXXXXXXXXXXXLKVISNDAKWFLLSKGSTYKWDTCAPHAILNSLGGGVLDLKDSLNEKSVIPLIYSEGKANSNGIFAYQNESQIQELFELNLEKTKHNIPSYMSDSHLLYAAPIWTSQNVTLRTINNVIHNSSRSSSRGNEIVSSKSVDVAAFSAKCVSSRSPLQNLIKMSINLVKLLINCSEKSANIARKIRADSSLLSLLTQEKSEEEANPRFVQDFKTLADVLIQETIRHDISGVFPEEANPRFVQDFKTLADVLIQETIRHDISGVFPDLKDNILGEESSEFTNSLGDSVCVSIGSTLEETQNCLEQVLDGNSTAAKILADEVHRNVDYDAGNIPDLPNLDYKDLGIWIDPIDEEMMHIDLDETTFCPTMRITIESNFVFYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GW76</accession>
    <name>T1GW76_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General transcription factor IIH subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02153970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02153971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GW76"/>
    <dbReference type="STRING" id="36166.T1GW76"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008048-RA">
        <property type="protein sequence ID" value="MESCA008048-PA"/>
        <property type="gene ID" value="MESCA008048"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037467_0_0_1"/>
    <dbReference type="OMA" id="VCTCELL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000115">
        <property type="entry name" value="General transcription factor IIH subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="11">
        <location>
            <begin position="104"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="11">
        <location>
            <begin position="180"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008048-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="558" mass="63899" checksum="D64EE42D9968F06A" modified="2013-10-16" version="1">MTTSSEDVLLQMGEVRYKKNDGTLYIMNERIAWMVEHRDTVAVAHKFADIKMQKISPEGKPKIQLQVVLHDGNSSTFHFVNRNGLQAQLADRDKVKELLQQLLPNFRRKVDKDLEEKNRIFTENPSLLQLYKDLVITQVLSSEEFWNTHAKEYKQKNTVKKQDIGVSGAFLADIKPQTDGCNGLKYNLTADIIHCIFKTYPAVKRKHQDNVPSKMTEQEFWTKFFQSHYFHRDRITAGTKDMFAECGKIDDQNMKNAVQSGPENPLLDIREFEDVPLEEGFCSVNGDSAVANSGNIVHQNMIKRFNQHSIMVLNTCTETKPVPDTLQAAKQKAKESEEHLKSPPKKRKVVDKIVYEDLGEPIVDLNEPLKTMNGKEICNDKLNLTKYLHGPMTNLNNESYEAQKDLKEVQYEIQSDTESWGHRTSHKSLVSVTAAVNALGELSPGGALMRGFQDQSLDQLVPSDFEKELKNLYLALSGLLKQFWECFPPTKSELEAKAVRMHEALKRFKMAKLQPFEDRALTELSPLGSSLTLHLNQLLQRASSKFATWQERKNRHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWC0</accession>
    <name>T1GWC0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052795">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008093-RA">
        <property type="protein sequence ID" value="MESCA008093-PA"/>
        <property type="gene ID" value="MESCA008093"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2186938_0_0_1"/>
    <dbReference type="OMA" id="SANQCKN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008093-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="109" mass="12008" checksum="373DE808660CCC2D" modified="2013-10-16" version="1">MSNQCRSCGRDVNNNFLPYNSAYSTTANNNNNNNTSTINNNLHSSLNKDGHNNMKSKISYATRSMTSAMMNNSHSSSTQLKAKVMFGTVGSIKELHQKEKSTREGTIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWU0</accession>
    <name>T1GWU0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02139577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWU0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008271-RA">
        <property type="protein sequence ID" value="MESCA008271-PA"/>
        <property type="gene ID" value="MESCA008271"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064133_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008271-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13567" checksum="3A8927D717EA90D2" modified="2013-10-16" version="1">MDKWTENCVTYDNDISIFTDVSKDNIGTGSGDFCNNTSIEFTTKLPKNCVPGRNPRYKRNFFYQEIRNNFISDYVDSQAAIQALDSTFVNSKLVKSTTVSEYVGSLGMQFLKVTKKQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXR1</accession>
    <name>T1GXR1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02372833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008615-RA">
        <property type="protein sequence ID" value="MESCA008615-PA"/>
        <property type="gene ID" value="MESCA008615"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1877772_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008615-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="136" mass="16233" checksum="21E4F0EEB99AA45A" modified="2013-10-16" version="1">MILYGGWVVFLAKRDKIKRENYQRSLEEEARRHVMAKKRKLNSSTSSDTTTSTIVNEKETIKETIVKIPIEEDDESVKKKDQPHHHHYKHCCHKHKHHKTKSRSSSGSSHHNHHHHHCHHGHHGHNRHSQKKRMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXT1</accession>
    <name>T1GXT1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02101923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008636-RA">
        <property type="protein sequence ID" value="MESCA008636-PA"/>
        <property type="gene ID" value="MESCA008636"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1505157_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF83">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="19"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008636-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="20743" checksum="242DEBF720246CD5" modified="2013-10-16" version="1">MTMCPLCDKCDFWNIKETCFYSKITYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLAHVRKTRIDYVTNTKEPRAPFWRMKLPGTVFSFSVVLLLVALAFAAVIGVVIYRMCILTINVNFFEETMSTSQKMMLTTASAACINLAGTLAHTNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYE3</accession>
    <name>T1GYE3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008868-RA">
        <property type="protein sequence ID" value="MESCA008868-PA"/>
        <property type="gene ID" value="MESCA008868"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1708742_0_0_1"/>
    <dbReference type="OMA" id="ISCAKRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008868-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17715" checksum="4F37D3EA53685140" modified="2013-10-16" version="1">NCGSHWFQESKSAFSSKISCAKREELAQTDNVSPFLAISARSGVIYIEYAEGIAILVQDKFPNTLSKLLSQALRIVKAWTIENRLGVNANKSNLVMFTRRHTMVDFKKPKLDRTMLKIKQKAKKLGIILDKKLSWRSSKEKRRFLGSKWVLRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYN5</accession>
    <name>T1GYN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BMERB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008971-RA">
        <property type="protein sequence ID" value="MESCA008971-PA"/>
        <property type="gene ID" value="MESCA008971"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560901_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BMERB" evidence="1">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008971-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9334" checksum="754BDF0B259DF013" modified="2013-10-16" version="1">MILITFLRSAPIIDCLETMEMLSRREKKASDLEKKLRAVMGGNSSNKETEEQLLAQWFTLINKRMLFCGGKCVNVPDLQATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ74</accession>
    <name>T1GZ74_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ74"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009172-RA">
        <property type="protein sequence ID" value="MESCA009172-PA"/>
        <property type="gene ID" value="MESCA009172"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1519589_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF56">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20263" checksum="A17A923F8AA42B60" modified="2013-10-16" version="1">MLVQADGIVYPSYFNEEFFTESLENGLDEPMVSIQTVTFEMGSTPGENYCSQIFRIRVTYRRKKSEKLYTTSLIVKSLLSGDEMDFLEHIDSFSKERTMYMKMLPVMEVILNGTKLAPKCYYTQKNPTGIYVFEDLGALGYKLANRQTGLDLPHCEIAMHKIENSMHPLWSWLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ98</accession>
    <name>T1GZ98_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ98"/>
    <dbReference type="STRING" id="36166.T1GZ98"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009196-RA">
        <property type="protein sequence ID" value="MESCA009196-PA"/>
        <property type="gene ID" value="MESCA009196"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031676_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009196-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="29249" checksum="850A5443B94C3423" modified="2013-10-16" version="1">MNELSTFLSLDAERILQEQNKKKSLIFGCVIPQVEVTLVMPSQTPGKETSGDTESVILPDSASLGDDLHLNHSSNWHPQNMDGFRHNTFGSVETPSPITNDGPSSFEMIGGISGSINIPTNPNTHGYNVQIQSTPTNSNTASQISKGDTGNFSLTNSIKSISKTTKNSTTDLSISKEINSGLLSMKKGFSSFMTSIDSAIKSGASTNDDLMSDTMSIRSDISSDSENISFIFNDDKTTDCIDVMFKLNPFTNDTIKSSPVEVGSEVWRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ99</accession>
    <name>T1GZ99_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02165695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ99"/>
    <dbReference type="STRING" id="36166.T1GZ99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009197-RA">
        <property type="protein sequence ID" value="MESCA009197-PA"/>
        <property type="gene ID" value="MESCA009197"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847403_0_0_1"/>
    <dbReference type="OMA" id="KSASHGC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF548">
        <property type="entry name" value="GH12235P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="66"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009197-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="16106" checksum="FB952267E0C614F4" modified="2013-10-16" version="1">MLRVREFIRFHQIPNPLRQRLEEYFQHAWSYTNGMDMNSVIKGFPECIQADICLHLHRSLFSNCNAFDEVSPGCLRALSLKFKTTHAPPGDILVHKGDALNSLFFVARGSIEIVREDMSRVVLGNYNFVWEDCKYKLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZI3</accession>
    <name>T1GZI3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02171347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZI3"/>
    <dbReference type="STRING" id="36166.T1GZI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009284-RA">
        <property type="protein sequence ID" value="MESCA009284-PA"/>
        <property type="gene ID" value="MESCA009284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106531_1_0_1"/>
    <dbReference type="OMA" id="WAVFIRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF37">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="31"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="19024" checksum="9680216B2E4B4BE3" modified="2013-10-16" version="1">MDTVEMEQRIVEKRANMEEALAATGFGKFNYILITISGFILLNVVLELLAISYVLPILAKCDLMLTVLQSGFLGGVGVLGITLTSHLWGYLADTIGRRKIIAPTLLLAFGFTLISSFSTNFWCLLIARFCNGCLVSASSATIYAFLGEFHSEGHRSKAVMWAVFIRTFMGILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZK8</accession>
    <name>T1GZK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WASH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02146465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZK8"/>
    <dbReference type="STRING" id="36166.T1GZK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009311-RA">
        <property type="protein sequence ID" value="MESCA009311-PA"/>
        <property type="gene ID" value="MESCA009311"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029156_0_0_1"/>
    <dbReference type="OMA" id="FTHMSES"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:gamma-tubulin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="WASH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021854">
        <property type="entry name" value="WASH1_WAHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23331">
        <property type="entry name" value="CXYORF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23331:SF1">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11945">
        <property type="entry name" value="WASH_WAHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="4">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="181"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009311-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="249" mass="26969" checksum="61B9DC50E186C215" modified="2013-10-16" version="1">METLASRKNPKANNGGTVESPNQKLEAAPNSITNRQYSCDKSQRQYTGIFYSPGVAEAPKLELPLDLPDLPGVAGDVQFGFMENLSVMNQADDEINFPPVSIVQTQTSVPEVSLIPPPPPPPVATSNYSLIPPPPPPPTPAVLSEQQISQKSTVVSAERPAHPKTDIPDARSNLMAAIRNAGGRPKLRAAADLERKETPKKPKENKGMDLMTDLHNKLMMRRKGISGAQKEEPQSVKDRLSSMIPAPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZL9</accession>
    <name>T1GZL9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZL9"/>
    <dbReference type="STRING" id="36166.T1GZL9"/>
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        <property type="protein sequence ID" value="MESCA009323-PA"/>
        <property type="gene ID" value="MESCA009323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1717812_0_0_1"/>
    <dbReference type="OMA" id="MFYCEND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028796">
        <property type="entry name" value="BBS8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44177">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44177:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="153" mass="16604" checksum="44BE62BDD77656EE" modified="2013-10-16" version="1">DLETNDNGEENEEFEFERLATAARPGTSIKTAFQPRPSTGLLAGSLRTGRKGTITAGNGRPMSGMLRPGTTSSRPGTSLGRPMSRCGTARRVRMTSAAAFGIGDKTSSFFQAARLNPTVYAERPTITRSIFLFLYYHEGDIDKAYTLCDAVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZQ0</accession>
    <name>T1GZQ0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZQ0"/>
    <dbReference type="STRING" id="36166.T1GZQ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009354-RA">
        <property type="protein sequence ID" value="MESCA009354-PA"/>
        <property type="gene ID" value="MESCA009354"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2489739_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009354-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="87" mass="9430" checksum="EC9D4B4D0922C828" modified="2013-10-16" version="1">MYKKDFSIRKVPEIVAESGSALLHFFSDDAYNMSGFNISYKVNGCPTNDSRVECSGFGACMDDGTCSCENLYTGEACNIALCPNNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H067</accession>
    <name>T1H067_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Angiotensin-converting enzyme</fullName>
            <ecNumber evidence="6">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase M2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02167293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H067"/>
    <dbReference type="STRING" id="36166.T1H067"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009535-RA">
        <property type="protein sequence ID" value="MESCA009535-PA"/>
        <property type="gene ID" value="MESCA009535"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1647947_0_0_1"/>
    <dbReference type="OMA" id="EIMATSN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008241">
        <property type="term" value="F:peptidyl-dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001548">
        <property type="entry name" value="Peptidase_M2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514:SF40">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00791">
        <property type="entry name" value="PEPDIPTASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009535-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="19311" checksum="0A4E8D6128369E28" modified="2013-10-16" version="1">MARSRDWDELQHIWLEYRRKSGREMRDNYAQLIDVMNEIAYANNITNGGEYWYSAFESYNFREDLERVWEEIKPLYEGLHAYVRRKLREYYGPDKINRNAPIPAHILGDMYGQSWSHILDIILPYPGRNFIDITPKMVEELYTPPTLFQVAQDFFASLNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H071</accession>
    <name>T1H071_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02384193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H071"/>
    <dbReference type="STRING" id="36166.T1H071"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009542-RA">
        <property type="protein sequence ID" value="MESCA009542-PA"/>
        <property type="gene ID" value="MESCA009542"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673908_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009542-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8703" checksum="CEDFAC4CB7165B1E" modified="2013-10-16" version="1">MVPIHASFGLVNFMLAIATCITGLIEKEKELSAEKSIRRYEDDSENAWNCGSWFEYLLIVSYQQFCLSEFWSKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H082</accession>
    <name>T1H082_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">XPA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPA family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02384434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H082"/>
    <dbReference type="STRING" id="36166.T1H082"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009554-RA">
        <property type="protein sequence ID" value="MESCA009554-PA"/>
        <property type="gene ID" value="MESCA009554"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053731_1_0_1"/>
    <dbReference type="OMA" id="MERHEHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022652">
        <property type="entry name" value="Znf_XPA_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01286">
        <property type="entry name" value="XPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="10">
        <location>
            <begin position="87"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009554-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="226" mass="26360" checksum="EAA9D8B68F822ADE" modified="2013-10-16" version="1">MYTATSSVIKVSGSKFVDSGGGFLVEETAVKEAITKPKEYKKVINDGEELPIKVTDCRECGDSFAGSYLWDNFDYPCCDKCHDKEGKHDLITKTDSKDEFLLKDCDFDLRQPVLRFVSKKNPHYKRGEMKLYLKLQVYKRAMEVWGSEEELERKKEERAEKKDAAKVKRFDKKLKELKMNMRSSVFTKDVAASHQHEYGEETYHEDDDNYSHTCKICGFYETFEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0I5</accession>
    <name>T1H0I5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0I5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009664-RA">
        <property type="protein sequence ID" value="MESCA009664-PA"/>
        <property type="gene ID" value="MESCA009664"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1241368_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009664-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="223" mass="24836" checksum="BA71BF0DBBE0731A" modified="2013-10-16" version="1">MEDNSAAFAASSPNASNAFANETKTTSNPFPDQSEDHYDSSHSGGIGQADSLLVNAEMMENAVLDIISNEVKESRDPSSVENQLMRELSVRKAVEPGSSSTRQVEDDLLEQLTNLKSKTSSLQEAKSRNLPKSEKEDIVNDIELEKRTRILEDEHLSNISKNIRSQPNISVEPPPEDEEIPKILEIRVLKVQLVDNNPRINRRLPEGVSTFYDFNTSASREPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-03-27" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0K4</accession>
    <name>T1H0K4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02178602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0K4"/>
    <dbReference type="STRING" id="36166.T1H0K4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009684-RA">
        <property type="protein sequence ID" value="MESCA009684-PA"/>
        <property type="gene ID" value="MESCA009684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032408_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF4">
        <property type="entry name" value="SIDESTEP, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13420" checksum="26A0F96D6A904CBC" modified="2013-10-16" version="1">PRACELRNDTVLEVICLAGSDGGLSQKFLLEVISTNSLNFNNDGKAIISSIDSEVDNEISTMNDQATSSPLFRMEEIRPQFRLDGLEPGREYQLQIYGVNAKGRSNPPVIIDKVRVSPQLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H164</accession>
    <name>T1H164_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02147113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H164"/>
    <dbReference type="STRING" id="36166.T1H164"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009918-RA">
        <property type="protein sequence ID" value="MESCA009918-PA"/>
        <property type="gene ID" value="MESCA009918"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932817_0_0_1"/>
    <dbReference type="OMA" id="VKVQCNT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF100">
        <property type="entry name" value="FASCICLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="41"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009918-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="131" mass="14502" checksum="148BD0B95AF3B85F" modified="2013-10-16" version="1">NLHINNSREEDQGKYECVAENAVGTEHSKAQLYVKVRRVPPSFSRPPEPLNEVMLGADLNLSCIAVGSPMPHVKWMKDHVDLTPENEVPVGKNVLYLKNIQQSANYTCVASSSLAQIEQTATVKVQCNTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1B4</accession>
    <name>T1H1B4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1B4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009973-RA">
        <property type="protein sequence ID" value="MESCA009973-PA"/>
        <property type="gene ID" value="MESCA009973"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392228_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009973-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="11262" checksum="C1F7D1867B74CBEB" modified="2013-10-16" version="1">MIMRLWAHRILKDGKKFIRLADMVLSTIWNTCCLMVQTWMAITLLVIHRCMYALLTIRKLVLECFYFEVPNGVHLIMPIKLLIRISKHFWEKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H257</accession>
    <name>T1H257_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pantothenate kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H257"/>
    <dbReference type="STRING" id="36166.T1H257"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010289-RA">
        <property type="protein sequence ID" value="MESCA010289-PA"/>
        <property type="gene ID" value="MESCA010289"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011154_0_1_1"/>
    <dbReference type="OMA" id="NEDYEIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24122">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000244">
        <property type="entry name" value="pantothenate kinase 1-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF30">
        <property type="entry name" value="FUMBLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010289-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31280" checksum="3C150AA4C9CF8E3A" modified="2013-10-16" version="1">MDDVEIRNRTGSLHFIRFPTSEMSNFLSLAKSKGMADLVTAVCATGGGAYKFEKDFMHELNMKLAKFDELDTLIKGVLFSELRNTAECYYFHNARNIRHSEKKSYDFSKPYPFILVNVGSGVSLLAVYGPNNYKRISGTSLGGGTFLGLCCLLTGFKDIYGGDYARFGLPGDLVASSYGQMHNKDKQGKVSREDLANATLVTITNNIGSIARMCALNEKIDKVVFVGNFLRVNSISMKLLAYAMDFWSEGSLKALFLNHEGYFGALGCLLQFNNSDIAEYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2H2</accession>
    <name>T1H2H2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="17823" checksum="1E9DDB2FC7890362" modified="2013-10-16" version="1">IFRQAKSFFDNPNIEVVGGVSLLKSNEGRQGKAIQDNSIAVETAPTVEARNSEMGTYFMDSAKKFFAERSLNLNLVDTARSVARAIPDDIKADISEIVTEARTLKKKKSLKKLLPLLKIVGVKTKGVRESCGKMSSDPADLYDLKLFIAHFNLSYETILPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010435-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="30" mass="3538" checksum="AB14FAEF3EE3934B" modified="2013-10-16" version="1">MFSTFDQGPKLCPTRVRRIPRTFTTLCYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="33" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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</entry>
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    <evidence type="ECO:0000313" key="2">
        <source>
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</entry>
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    </protein>
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            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
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        </location>
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        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="123" mass="14026" checksum="5A1FC8D42A81C6E9" modified="2013-10-16" version="1">WCDLLVAGLNWLHASLNSSAIVCGFLHYGKLAGTSIGIGMFITHSIIAKIYLKKFNNLNLPFWIAMNTIAWFLMTTLLLLALATEFKLCDLMYSITFTTSSLIFHQFYWFSIIKQFPLCCTRS</sequence>
</entry>
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    <accession>T1H3E2</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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            <authorList>
                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3E2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010767-RA">
        <property type="protein sequence ID" value="MESCA010767-PA"/>
        <property type="gene ID" value="MESCA010767"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592480_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010767-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="9319" checksum="72ACC4E7D026307E" modified="2013-10-16" version="1">MGRTSKRQAKNTLNCVKAAGGISEKFKTQGVSVKDMSCRWNLTRELLEKYILAMKLYREPREKYSERCSRPSLSHFAKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H421</accession>
    <name>T1H421_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H421"/>
    <dbReference type="STRING" id="36166.T1H421"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011020-RA">
        <property type="protein sequence ID" value="MESCA011020-PA"/>
        <property type="gene ID" value="MESCA011020"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392222_0_0_1"/>
    <dbReference type="OMA" id="SAQHTKC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051295">
        <property type="entry name" value="LGI_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24367:SF318">
        <property type="entry name" value="LEUCINE-RICH GLIOMA-INACTIVATED PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24367">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01463">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LRRCT" evidence="4">
        <location>
            <begin position="44"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011020-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10536" checksum="F2ADDEEBACCA4E7E" modified="2013-10-16" version="1">MDEQAFKGLSNLEILTLNNNNLTTMPHNVFNGLGRLRALRLSENPLACDCHLSWLSRFLRNTPRSAQHTKCQSPSQLKGQNVAELHDADFKCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1H437</accession>
    <name>T1H437_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02191204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H437"/>
    <dbReference type="STRING" id="36166.T1H437"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011038-RA">
        <property type="protein sequence ID" value="MESCA011038-PA"/>
        <property type="gene ID" value="MESCA011038"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340510_0_0_1"/>
    <dbReference type="OMA" id="TSGICDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF90">
        <property type="entry name" value="SEMAPHORIN 2B, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sema" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011038-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="205" mass="24025" checksum="7FF29540C03DF3B2" modified="2013-10-16" version="1">IFYSYPYFHPCRVRIDKLNQEYLIYFAGTNTGSIYKIVQFIKFGQRQSVLLDILDISKNEPIREMEISQKTGSLYIATDHSVKQIDLAMCNRRYDSCFRCVKDPYCGWDKEYGACKPYQLGLLQDVGNETSGICDSSVLKKKITASYGQTLHLSCFVRLPEVLRHNRSNGCIIQRKKEGKWKLWMHFTLYIHSWKVVDSVDFCWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4T1</accession>
    <name>T1H4T1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02127399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4T1"/>
    <dbReference type="STRING" id="36166.T1H4T1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011299-RA">
        <property type="protein sequence ID" value="MESCA011299-PA"/>
        <property type="gene ID" value="MESCA011299"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1647944_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011299-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="161" mass="17160" checksum="E89BC94E342A6C8C" modified="2013-10-16" version="1">SPYTSYENGVTTAGLSLSIAKANGYTPSSTYSPRTTTSRLANNGKSLSISNSSLNSYTPNTSSPSTAPTTSVSVPSAASIMVSRSSSLREHERKSRNRTRSRNIAQRSLSASSEKSEGYESGTERTSRSRLGSTNSLADGDNKQSNENGEIDYKALWEAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4T7</accession>
    <name>T1H4T7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02161983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4T7"/>
    <dbReference type="STRING" id="36166.T1H4T7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011305-RA">
        <property type="protein sequence ID" value="MESCA011305-PA"/>
        <property type="gene ID" value="MESCA011305"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707632_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011305-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8463" checksum="FCFE1A0419F3961C" modified="2013-10-16" version="1">MEKFDTPIKINDLSFDSGVLAPSDMKYGFRHEKPFNFIYIEMENDAGEFVDLYCLRKCTSSHRIIHAKDNASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4U0</accession>
    <name>T1H4U0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02384263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4U0"/>
    <dbReference type="STRING" id="36166.T1H4U0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011308-RA">
        <property type="protein sequence ID" value="MESCA011308-PA"/>
        <property type="gene ID" value="MESCA011308"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013516_1_1_1"/>
    <dbReference type="OMA" id="RYEESIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011308-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="163" mass="18124" checksum="1E8082DB5A323C22" modified="2013-10-16" version="1">MFLEVVQKTANQVDPDIQEALGVLFNLSSEYDKAVDCFQAALNVSPNNAKLWNRLGASLANGSRSVEAVEAYQRALELEPGFIRVRYNVGICCMNLKAYNEAAEHLLTALNLQATSSERSGLDVTKDKKQQMSNSIWSTLKMIISLMGRNDLHELVNIEMLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>T1H554</accession>
    <name>T1H554_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02024204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02024205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H554"/>
    <dbReference type="STRING" id="36166.T1H554"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011429-RA">
        <property type="protein sequence ID" value="MESCA011429-PA"/>
        <property type="gene ID" value="MESCA011429"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264375_0_0_1"/>
    <dbReference type="OMA" id="YEMERMH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022843">
        <property type="term" value="F:voltage-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031649">
        <property type="entry name" value="GPHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16905">
        <property type="entry name" value="GPHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011429-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="219" mass="25017" checksum="085517677FB413CB" modified="2013-10-16" version="1">MSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKNGIDDVYNFQTFGQSMILLFQMSTSAGWDGVLDGIINEVECEKPDVDRGITGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5F3</accession>
    <name>T1H5F3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02179160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5F3"/>
    <dbReference type="STRING" id="36166.T1H5F3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011530-RA">
        <property type="protein sequence ID" value="MESCA011530-PA"/>
        <property type="gene ID" value="MESCA011530"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061880_0_0_1"/>
    <dbReference type="OMA" id="TCYLQVS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF174">
        <property type="entry name" value="TYRAMINE_OCTOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="206"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011530-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="403" mass="45429" checksum="652CC6B79E80435C" modified="2013-10-16" version="1">MIAAGSVGIGSRFDEQRLTLRIHRGRGSHQMEPAHSNGSSHSSTTTLGTPSPERFSRYSTRRTQQTPSLLHTFHSHVHHHHATNPPQHEKIKISVSYPSNENIKDIATGKASSVAEAVNKQEGPNPLLYAVHFTSPKTGQSTTESQLYRTTKTTNNNNSFLKVIDRSPSTDQQQSPTQTDMCGHRTTNKKMGKRNIKAQVKRFRMETKAAKTLAIIVGLFIFCWLPFFTIYVIRPFCEDCIDPLLFSILFWLGYCNSAVNPMIYALFSKDFRFAFKRIICKCSVIIRVCPKKLVRVEGVPICLTSNLGVVLQVLHRVEPPTPLEMKVRMMARRRSSSIGLSGKARKQSLKAPVYEICHPLVMDSSFSYNSTGNTQTTVFHMEEDKLPESSTQKTKFSSKLTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5X9</accession>
    <name>T1H5X9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">hypoxia-inducible factor-proline dioxygenase</fullName>
            <ecNumber evidence="7">1.14.11.29</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-prolyl-[hypoxia-inducible factor alpha subunit] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[hypoxia-inducible factor alpha subunit] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:48400"/>
            <dbReference type="Rhea" id="RHEA-COMP:12093"/>
            <dbReference type="Rhea" id="RHEA-COMP:12094"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.14.11.29" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="T1H5X9"/>
    <dbReference type="STRING" id="36166.T1H5X9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012112-RA">
        <property type="protein sequence ID" value="MESCA012112-PA"/>
        <property type="gene ID" value="MESCA012112"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138287_0_0_1"/>
    <dbReference type="OMA" id="NKMNDNG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160082">
        <property type="term" value="F:hypoxia-inducible factor-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051559">
        <property type="entry name" value="HIF_prolyl_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907">
        <property type="entry name" value="EGL NINE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907:SF26">
        <property type="entry name" value="HIF PROLYL HYDROXYLASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="9">
        <location>
            <begin position="65"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012112-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="20279" checksum="EDE756DC0FA9A55D" modified="2013-10-16" version="1">DGQVTAHSKEGRNLKAIRGDKIAWVKGSQDGYSNIGYLINQIDTVICRANKMNDNGQLGNYSIRERTKAMVACYPGSGTHYKMHVDNPNKDGRVITAIYYLNVDWDTKRSGGVLRIFRKQDDAVADIEPKFDRLLFFWSDIRNPHEVQPAHRTRYAITVWYFDANEREKARNRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6G4</accession>
    <name>T1H6G4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DZF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H6G4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012297-RA">
        <property type="protein sequence ID" value="MESCA012297-PA"/>
        <property type="gene ID" value="MESCA012297"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_699398_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006561">
        <property type="entry name" value="DZF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049402">
        <property type="entry name" value="DZF_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052134">
        <property type="entry name" value="ILF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46447">
        <property type="entry name" value="INTERLEUKIN ENHANCER-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46447:SF1">
        <property type="entry name" value="INTERLEUKIN ENHANCER-BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
        <property type="entry name" value="DZF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00572">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DZF" evidence="5">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="395" mass="44097" checksum="AFF5795014BD619D" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPTNEAVEALSKKVEADLKNAMKTEVVTKAEQITITINDRGFDIANASAKVRVLISTLHQNIRKLESDIHLDPIVMQSNLSAIRHTRWFEENAHHSSIKVLIRILKDLRVRFDAFVPLSPWMLDLLAHLAIMNNPNRQALPINLAFRRVFQLLAAGLFLPGSAGITDPCEPGHIRVHTAMSLEQQDVCCMTAQTLLRVLLHGGYKHILGLEGNTSIVREMSVWNGVTVRPLNPVYEAPADKKDGEVDDMETVETDGFGEEEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6M1</accession>
    <name>T1H6M1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6M1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012354-RA">
        <property type="protein sequence ID" value="MESCA012354-PA"/>
        <property type="gene ID" value="MESCA012354"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275062_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF177">
        <property type="entry name" value="ANNEXIN B10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0041">Annexin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012354-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="217" mass="24479" checksum="65CC946085E96C3C" modified="2025-10-08" version="2">MXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWYANRYDRPLSEHMCITSGNFCRLLIMIVTGTIDPVTTDPEQAKEGSDEEVFKKILHSNFAQTPAAFFANQLHKAMDGAGTDDDRLIRTIVSRSEIYLAKIKDEFKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6P1</accession>
    <name>T1H6P1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inner dynein arm light chain, axonemal</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6P1"/>
    <dbReference type="STRING" id="36166.T1H6P1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012374-RA">
        <property type="protein sequence ID" value="MESCA012374-PA"/>
        <property type="gene ID" value="MESCA012374"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072652_0_0_1"/>
    <dbReference type="OMA" id="QVTIICA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="131"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012374-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24376" checksum="C492D2D2B695CC57" modified="2013-10-16" version="1">EVKRPHTSVILDVKRETEEILNSIIPPRCWEEDGQFWTQTVSSTPATRQDVINLQELLDTRLQQTQARETGICPTRRELYSQCFDELIRQVTINCSERGLLLLRIRDEIAMSMEAYETLYCSSVAFGMRKALQAQEEKERLRDRVKTLEVDKDSLEDIIADMKIKSEQAERRNAELRASEEKKFAEEVAFLKKTNQQLKAQLEGITAPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6P8</accession>
    <name>T1H6P8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6P8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012381-RA">
        <property type="protein sequence ID" value="MESCA012381-PA"/>
        <property type="gene ID" value="MESCA012381"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1323782_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012381-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="208" mass="23128" checksum="F8C024409E5CADF1" modified="2013-10-16" version="1">KLKMLDITRDKPIKVAVKVAVPVKDHPKDYHDDIRQEQMWEMQALTSTPSTNNMTGDQNSPTNSVDMDITTDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGNMGRKRPLLGVPRSGMNPTKRTVMTLLARAKHSQSLHPNRNGTTGGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H732</accession>
    <name>T1H732_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 7">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H732"/>
    <dbReference type="STRING" id="36166.T1H732"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012514-RA">
        <property type="protein sequence ID" value="MESCA012514-PA"/>
        <property type="gene ID" value="MESCA012514"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105983_2_0_1"/>
    <dbReference type="OMA" id="CLDANKK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000002">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012514-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9531" checksum="9A0AE8D02D051ECE" modified="2013-10-16" version="1">MGRRKSKRKPPAKRKAIMPLDQQFNCPFCNHEKSCEVKMDKSRNTARITCRVCLEEFSCTINFLSEPIDVYNDWVDACESAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7K0</accession>
    <name>T1H7K0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pseudouridine synthase II N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7K0"/>
    <dbReference type="STRING" id="36166.T1H7K0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012737-RA">
        <property type="protein sequence ID" value="MESCA012737-PA"/>
        <property type="gene ID" value="MESCA012737"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_1_1_1"/>
    <dbReference type="OMA" id="SGISCIR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039048">
        <property type="entry name" value="Trub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13195">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13195:SF0">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012737-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="172" mass="19784" checksum="C1B8AC10C9E0B63C" modified="2013-10-16" version="1">LGLKKCAKLAISLRHQRFLRTYHVRGMLGVSKENYFWDSRVTCKSKYNHVTEARFSSIVASLQASHQRKMYDMCGVDIQSQAGYELALKGVIRPENNQEPIIYGIRLIEFKPPSFTLEIHAINEDEQFLAMLLHDIAIEMKSVAHCSGISCIRYGPFTLENSLLRRHWNLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7L3</accession>
    <name>T1H7L3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HID1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7L3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012750-RA">
        <property type="protein sequence ID" value="MESCA012750-PA"/>
        <property type="gene ID" value="MESCA012750"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_385096_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026705">
        <property type="entry name" value="Hid-1/Ecm30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575:SF12">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12722">
        <property type="entry name" value="Hid1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012750-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="718" mass="81902" checksum="3A2394D8C481C443" modified="2013-10-16" version="1">MGNTDSRANFNKAFEQLLAKNNTIDPDDDEFWSKFWQIPQLQQIFTSVSTNDIRRLRKENPTNLSTLCYKLVERLAVAVDNSCRTSAEQQACMSCVHILLRILPYVFEEEEWIEFFWLRSQGKIILFAESLINTVCDLLFCPDFTVSSISRYGSPDKAEELANIDSCEYIWESGVGFATSPPKNSYMEQRRKELLQLLLTCFSESMYRSQKKHNKWIAYFTSSENRHAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEWVQSWRSKLPLQTIMRLLQVLVPQVEKICIDKGLTDETEILKFLQHGTLVGLLPVPHPILIRKYQPNSETTDWFRTYVWGVIYLRNLDPSIWYETDVKLFEIQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9X7</accession>
    <name>T1G9X7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Titin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02159541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02159544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1G9X7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000019-RA">
        <property type="protein sequence ID" value="MESCA000019-PA"/>
        <property type="gene ID" value="MESCA000019"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_230627_0_0_1"/>
    <dbReference type="OMA" id="PRERENF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd11856">
        <property type="entry name" value="SH3_p47phox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000080">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001138">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF100">
        <property type="entry name" value="FASCICLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="1394"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1486"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1609"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1691"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1785"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1880"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="2036"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="2135"/>
            <end position="2219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="230"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="369"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="576"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="667"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="838"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="865"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="909"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="979"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1082"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1246"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="576"/>
            <end position="607"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="698"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="708"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="737"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="802"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="918"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1096"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1247"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1280"/>
            <end position="1298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000019-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2253" mass="259402" checksum="E4FD660DD5473AE9" modified="2013-10-16" version="1">VEAPQEEFDIHKFKITESEIVDTQVEEKPELEEKPIIKKSKKPKVDEKEYEFQSPDQEISLAEKEPSEEVTEVESKPKKRTHKHKKDEETPEYAIVGIKEFEKPEEEIPSEDIKIVEGQDKKEDISFEPEEITVKDIIDDQGVEKKQITKKRTIKKKDGDKEHTVTITTVQEEDKPEEASVTITEEVVPIEESPKQEKPKKRVKKVEKTIPKDEVDDYIQKLIEQEIPKTELEQYTKFEFEEKPKDKEIDQDQPVEVSLKEPKSKLESEPETEQEKPKKKPKSVKEPKPDIVKVIEHEETKEQSQEKDIPQVEDTIQPIQEQEHPEDTASVPSKKSKSKQTKLPKDITHTDASESIEEYKISVREEIIPEIEEIEEEQPEIEQPVITEEEEQPESQEIDVVEEEAPTQPDDKPVDKPKKPKKKAVKKKTDDHDEIIKALLEQDVERTVLEKYEKVELEKRPKKEKPKIEGLVPQKIVRTEHKPQKVVIVEASEPTEDTRVQLPGFKLKSRIIKVEYPPAPLIPKITDIGAVKAQGELSRNVDEAEEKLKFQKPKKFKPMKKIKDDLEKPELEKYEKYVESSEEEPEKKPYERPIKEKPQEEEKKDITLKISKGKKKPIQEEEMEEVKLKKIPLKKPEEVEVEERIPKKKEIEVPKEEDVKEEIKVNLEPFQPIEIEEHTTTQEEYEAPEWPEKEKKPKKEKKPKKEKKPKEEIPEEEKEHKIVLGKGKPQEEEVEEEVKFRIPEQGKPEEEKPEEVKLKPFKKPEPTPEDKTYKIKTKEFEAPKVEQPEEEEAVEIPQIEEQIPEEVEADYGDEFQVKVTTHEPKEIEWEEVIEERTVTRKKKPKPVQPQQEEPVEDYTFKLGEPETVEEQFSVPVKEEQPVEEAEAELDVKVEEEQEEVKVIEVVEEEVKKKKKPKKPQEIPKPEKVEIVDEEPTPVEEPLKIEEVTEDVEEKYDEHQINVIDVEAVDDIVEEIPEEVPISQPEILEEEVEQPQEQYEIGVKEEKTVEVEEVIEERTVTRKKKPKSQTELPEEEFIAKLKEPEVVIPLDEAPEAEIVLKKEKVKPEEEDQAELEIQVKEIVQPEEVPEEQAPEEFTIKQEKKEPDQPEEVEAEISIPKQKPVVEEEAADELSIKVVEEVPQEEIAEEQVTLKKKKTKKPKVVEEAPEEFTVKEKKKPAQPEEIEAEISIPKERPVVEEEAADELSIKVVEEIPQEKEIIVEEEEEEPSQFIVKKSKPAPLPEEFEVEVKLPKQKSEELPAENVTIKKKKPKKQPEDEEAQHLEVQIKLEKEKPKEQPEEQLTLKKKKPKKVPKDEEAALELKQEVVEERPVEVVEEEIIDEAIVKRPRPKPKPIQPTYEDLSEEEFSVSVSRPKVHKINEGVEGEGSITKKQTEEQLVHAICNYVAENEEALNLVEGEKLYVVERHNSEWWFVKKTISEEHGWVPSQYLMEPVEYTHYVQKKLHEKIDKLPVFERPGSDEKATAPRFIEKLQPIHTPDGYTIQFECKVEGFPRPQIAWFRQTAIIKQSQDFQMFYDEDNVATLIIREVFPEDAGMFTCVAKNAAGFASSTTELIVESTTYSDHGSELLSRKSMSRESSLADILEGIPPTFSKKPKAQYVDENSNVILECRLVAVPEPDIIWTFNGKEIVTEKNVKIITESDMHMYCSVVHISKVQKSQEGVFETGEQEKPQILEPLRNMAIREGESVVLSTQIVGNPTPKITWSKDGKPIKENTKTDKNIHTLTLIAPKATEKGQYTVKAVNPLGEAETTAILTIEEPVSDGEPPFFVERFEEQFVPQKGTIRLTAKITGNPVPEISWLFKNNPLYPSDKVKQVYDGENVELVITEANPETDSGDYKCIASNPLGKISHGARVIVEVDEVIFTKKLKKTVTIEESQSLTLECETSHMVSTKWFFNNKELTGMDHRVVVEDGKVHQLVIRNTTLRDAGKYKCMVKDKTTESTVEVIPRKPEFVKPLQDYEVTENEIAILDVTPDTNPKVELVKEDEDEGEYTIKLTNPVGDVEDTLHLIILKPPSAPGAPQPVEVVDDSLTLYWRAPESNGNSEITEYILEYKTVKADKWTEIRKLKDTTHTLSKLVIDEEYVFRSIAVNEVGPSPPSPLSPPILIGKAKHEHEPTVQEPLQDTTSDLNKTVTLSCVFSGMPSPTVQWTKDKKIIESETMSYENYVAKYVITTTEDSEGTYTCIGKNKLGTVDTSCVLTIIEKPKISLEDSFLTLRTDSTLKIPSTFSGFPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAD6</accession>
    <name>T1GAD6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF753 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02094949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02094950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAD6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000191-RA">
        <property type="protein sequence ID" value="MESCA000191-PA"/>
        <property type="gene ID" value="MESCA000191"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059793_0_0_1"/>
    <dbReference type="OMA" id="NTAISCH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008472">
        <property type="entry name" value="DUF753"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721:SF26">
        <property type="entry name" value="DUF753 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721">
        <property type="entry name" value="GH09876P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05444">
        <property type="entry name" value="DUF753"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF753" evidence="1">
        <location>
            <begin position="18"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="1">
        <location>
            <begin position="94"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="1">
        <location>
            <begin position="192"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000191-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="373" mass="40657" checksum="774759FD239AF399" modified="2013-10-16" version="1">MFEPGCNKNEISTVAKISCFSCNASEDNCEIPEAKTCEQETTGCFTKFDSRSDIVEMGCKGVYSEQEVLNNLKSFLECEGDKCNDLKNIPNDGSCKVCDSTSDPDCIKNPGKLSDSLCGKPPHTSCYARLTEQNTVERGCTSNLDDSHFKDCVLKNDKNCFICSEKGCNIDDIKPADVAEDLVGVWQDLPVNCYHCKGDSCGPNGNWILRSCSENKYQTCSTVFGLTGEVIERGCSDTVQQSQADYCSTNPEKCLKCKSNGCNTFADASKLVECLSCDVNGDCSKTTQCDGKCMVAINPLTNGIYRGCLNDKELDDQDDCGVDGTCKACEGALCNDFDLEPIEDSVVIHAMMVIVSSLYLNDVINKLKKTNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAH7</accession>
    <name>T1GAH7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02000737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GAH7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000234-RA">
        <property type="protein sequence ID" value="MESCA000234-PA"/>
        <property type="gene ID" value="MESCA000234"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_247885_0_0_1"/>
    <dbReference type="OMA" id="KKYEHRY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577160" evidence="2">
        <location>
            <begin position="22"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1298"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1356"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1452"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
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        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
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        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="532"/>
        </location>
    </feature>
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        <location>
            <begin position="541"/>
            <end position="552"/>
        </location>
    </feature>
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        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="711"/>
            <end position="737"/>
        </location>
    </feature>
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        <location>
            <begin position="740"/>
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    </feature>
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        <location>
            <begin position="776"/>
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    </feature>
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        <location>
            <begin position="854"/>
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        <location>
            <begin position="880"/>
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    </feature>
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        <location>
            <begin position="910"/>
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        </location>
    </feature>
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        <location>
            <begin position="934"/>
            <end position="959"/>
        </location>
    </feature>
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        <location>
            <begin position="960"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="983"/>
            <end position="994"/>
        </location>
    </feature>
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        <location>
            <begin position="995"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1035"/>
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        <location>
            <begin position="1036"/>
            <end position="1045"/>
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    </feature>
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        <location>
            <begin position="1080"/>
            <end position="1089"/>
        </location>
    </feature>
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        <location>
            <begin position="1123"/>
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    </feature>
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        <location>
            <begin position="1152"/>
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    </feature>
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        <location>
            <begin position="1178"/>
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    </feature>
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        <location>
            <begin position="1212"/>
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        </location>
    </feature>
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        <location>
            <begin position="1222"/>
            <end position="1252"/>
        </location>
    </feature>
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        <location>
            <begin position="1274"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1356"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1371"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1454"/>
            <end position="1467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000234-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1518" mass="153448" checksum="9F3ADF09297879ED" modified="2013-10-16" version="1">MAPTKLGYWIIFGILFVGVLTLEQQQQSVDDNAVVGSKEYVEALSRFKRSPRGYSRGQTQSQYLNFGTQQNDGKAEAEATEGGSRSFVTGTNGMGQAHPSFQVAIYTTDNLGRLIKPGKHVYGPVGPDGKPGTINFNNKPGLSTTDSLGRPIHGQQGLTDSQGRPIHGQQGLTDSQGRPIHGQGLTDSQGRPIHGQQGLTDSQGRPIHGQTGLTDSQGRPIHGQGLGRPIQGQPELTDSQGRPIHGQQGLTDSQGLTDSQGRPTQGQQGLIDSQGRPLHGQQGLTDSQGIPRTDSHGRPLIGLDANGKPIYGNILNQPGFNGSEPANLIVPGTDINKIGSHSTGILSPGDSFESKKTVTLPGCGSCAPTQLVQLVQVLQGVPNGQASIRTPDGTHLIGTIPQDVINQGAGSQRYPGYTSQTQQGTPAGNIKTAPGIQGYPGSSSHSSGSGSKSYPGYISSTHPDSGHILGPETQTYPGHISGPGTQTYPGHVTTGQNYPGSGGQGQTASPTYTTSDSQGVTRPGTQGYQGSGVQVPGYTVGSGSDGYPGSSSQTPGQLNTGSVTQSLVSPTYPGAQVGGQIPSGSGNQGYPAQISGSPTYPGTGAQTPVGSGSQGYPGSNFQIPGQIGGSPSQGYPGQVSPTFTGSSSPVTAGSGGQNYPGHQAHLPGQTGSTSPIYSTGSGPHGYPGSGSEGTRYVPGQTGSGTQGYPGSGSQSLPGQISSTYPGYQISGQAPTGSGTQGYPGYVGPTYTGSGPQVTTGHTDQVHPGPGAVPGQIYSSLGSQGYTGSNGYPASSTQVPGQTPIGSGSQGFPGQVIPTYTGSQVTSGPGSQGYPGSTNHFSGQIPAGSGTQVYPGSSGQTPGQTSTGSGQGSPTYPGSQVTSGSLGYPGSTPQFSGQTPTGTQVYPGSNGQTPGQTSTGSGQGSPIYPGSQVTGSGSQGYPGSSSQFSGQIPIGSSSQGYPGSSTQIPGQIPTGSGSPGYPGQVSQTYPGSQVTSGPGSQGYPGSSNQFSGQIPTSSGSQGYPGYGSPTYPGTGSQVPGQISSGTGSQGYPGLSTSGTQDYTGYPGSGAQIPGQTISGYGTPGYTGYNGGPSPQVPSGSGSGYPLSSDVIPGSGQTGKLVSPGSVTVASTGVSGKDDAFSQAESSLKDGLASASSLGRKNGGTAKSQVSGSAAVGGTFSASAMTSDNDRSSSAQVQGTPEGAMTQAQGQGGVAQSQAQVQSNAKTGGTTATSQRGLADAQSSGPGQTSSQAQIGFRPRNENDNDVSVSSGGGTASAQSGSHSGQSQAQILGKSKLGVSYHGAAQSASGTKEQVDTYREQNKNLFQTISQFGSNGVTDQAASVAGEGYNTNLLKSKKDDKIQETVDNKVDSDLTDNYEDDYSDNYDDDYEDQPALETKSKTVEPKLFSPIVEATTQKIDNTFTQKSGTQNQQAAVANPLEPFSLVQKQNDHVNKYSVNPTTPSPSKVTTEIIPPGFRGTVNHEKKIHTKALSTPKSFEDNSILPANGKESKKVPVPTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAI0</accession>
    <name>T1GAI0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAI0"/>
    <dbReference type="STRING" id="36166.T1GAI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000237-RA">
        <property type="protein sequence ID" value="MESCA000237-PA"/>
        <property type="gene ID" value="MESCA000237"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944360_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000237-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6805" checksum="58C6178E7C46D2B0" modified="2013-10-16" version="1">MANSSPLCREDFDGGPISLDLIEPHLYLDLTKEDILQSLESCTEFIADAVEKEENILVHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAV4</accession>
    <name>T1GAV4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ell-associated factor Eaf</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes transcriptional elongation by Su(Tpl)/ELL. Essential for development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02116608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAV4"/>
    <dbReference type="STRING" id="36166.T1GAV4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000369-RA">
        <property type="protein sequence ID" value="MESCA000369-PA"/>
        <property type="gene ID" value="MESCA000369"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_710366_0_0_1"/>
    <dbReference type="OMA" id="DVSANNQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032783">
        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003711">
        <property type="term" value="F:transcription elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027093">
        <property type="entry name" value="EAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019194">
        <property type="entry name" value="Tscrpt_elong_fac_Eaf_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970:SF2">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09816">
        <property type="entry name" value="EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription elongation factor Eaf N-terminal" evidence="11">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="137"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="217"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000369-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="389" mass="40971" checksum="A30DF6FBC3F17818" modified="2013-10-16" version="1">MKRSGASSSMTIGGGVSGNGVGDGPSGASGSLNMDGQVRELKLGSTFNARNTTTAFHTIKYDFKPASVDFNKVATFNIESNNQVTVTVPNVEGSGVPNTVYQGVQRKYTKECLLIIDRDTGVVTLEKLNDNIMVKKTRKGQSGPARGPLPSLENSTKRISSKTKVSTGSRKTNSNIISAPQRSATNTAPTVMSMSPLQSTLGISGNTRSPIQAPEWKANAAPSTLPSVPMLLDDDDDAMMSYPPQNLFANNQRHSQSQSPGSSSKTVQPPQQQMKQASPQLPNTFSNNFSNSSNSSIFGAGANGASHLPVNGTTTTPPPTIPTMNNLGEDIGILSSSSSSGSDSESSSDSDSSDSDSDTEQPQATGMSMPSVPMQQKMVFHNGGCWFCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB75</accession>
    <name>T1GB75_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="5">2.1.2.2</ecNumber>
            <ecNumber evidence="6">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="5"/>
    <dbReference type="EC" id="6.3.3.1" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02199923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB75"/>
    <dbReference type="STRING" id="36166.T1GB75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000497-RA">
        <property type="protein sequence ID" value="MESCA000497-PA"/>
        <property type="gene ID" value="MESCA000497"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005361_0_1_1"/>
    <dbReference type="OMA" id="EEFSYLC"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000001">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000011">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.600.10:FF:000001">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="20">
        <location>
            <begin position="20"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000497-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1077" mass="116818" checksum="58F42FB783BEB79A" modified="2013-10-16" version="1">MGAYCPCPLITSAQLEFAKKEILQRALDGFKKDNIKYCGVLYAGLMLTPDGPKTLEFNCRFGDPETQVVLPLLESDLFEIFTACSENNLSNVELKWKNDVSAVGVIMASAGYPETSTKGCVITGVPKNTEDSIIFHSGVAVNAQNEYLTNGGRVLIAVSLQNDLKKASENALKIAQGIKFSGAGAQFRTDIAHKAFKINTGSLSYKDSGVDIDAGDNLVQRIKPLARGTHRQGVLGGIGGFGGLFNLRELKQYKNPVLSEVTNGVGSKILLALENNIYDTIGYDLMAQCANDVLESGAEPVAFLDYIACGKLEVSVAAQFVKGISEACRETNCALLGGETAEMPSVYASGTYDLAGYCVGVLEEGEDLPKFDKYEDGDFIIGLPSSGLHCGGLEQVHAIIERSKDLMAKLQQPAEFGNKSALLAQEILAPTKTYVKEVLPLIRNKQLKAVGNVSSCLQDAIKKILPSEIYGWLHGPLNISENSFLENFNCGVGMVLIASKSNANEIVKSIPGSSVIGFLKKNSNNITYRNFHEVLMKASAHFEKDTGAKIEIIKSEKPVAIKNSVENLLKENSKSENLILTQSGKRLTKIPAKYEEPILVIGTDGVGTKIKIAQQTEINSTVGIDLVAMCVNDILCNGAEPLSFLSYYACGNLNEKTVIDAVSGVIEGANQGNCVLVQAHIAEVPDLYADKVYDLAGFSLGVTEKSRILPKTEDIREGDIVIGLPSNGVHSNGFSLIHKIMNRAGKTFQDPCSFSNKSFGEEFLAPTKIYVKSLQELVEKKYIKALAHITGGGLLENIPRVLISNLGVHLDASKYVIPPVFGWLSANGNVESLEMQRTFNCGIGVILVVDPKYEAVIMDALKYSHRASKIGNVVKLAPGAPQVVVARFDENIKRVQRIIAEPRKKRFTMGLNSEVVVVVSNKPNVLGLERAQKAGIPNYFINHKEFKSRETFDDAVSQILEEHKVDIVCLAGFMRVLSAPFVGKWKGKLINIHPSLLPKHPGLHVQQKAIDAGDLESGCTVHFVDEGVDTGSIIVQERVPILPNDTAEDLTKRIQKAEHYTFPTALRLVSTGLSKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBR1</accession>
    <name>T1GBR1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02185397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBR1"/>
    <dbReference type="STRING" id="36166.T1GBR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000698-RA">
        <property type="protein sequence ID" value="MESCA000698-PA"/>
        <property type="gene ID" value="MESCA000698"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_571859_0_0_1"/>
    <dbReference type="OMA" id="NSHANHF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF393">
        <property type="entry name" value="CHROMATIN-LINKED ADAPTOR FOR MSL PROTEINS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="362"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000698-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="478" mass="56463" checksum="CFA888D9893710BD" modified="2013-10-16" version="1">TQTKKKYKAEEEKPTISPTRRSTRHQKEEIKEEIVYPDEEKNLSELAEISLEEQSEDDYEPADDAYDPPNEEPSSNSESELDLDEEFGIKKGKKTKRKTAKDSLETDNTLEAKPKRKYKKLTPEEREAKKNKSSSKQMKLCAEWDQFLADFGFVFKCKICDFVGKDFRDLKGHFRVAHDKPRGYAMCCDRIFYKKEVLVEHIHIHINPEHFKCKNCGMTFKASKSLEFVGPKDLDSHIKRMHEMISSTLCEQCGKVVQNSKMSAHRLEHQGKKPEMIECHLCGAKLRCDLNRHIEAMHSNSDEKHVCHVCGKESSNFYALRKHILYNHQMERKFECTICDKKFKQRYNLKEHMAVHTGESLYNCPHCEWRFKSSANMHTHIKRRHRAEWEENRKYRNYSKKHFKCKNCGMTFKASKSLEYHSIHTCKSINSEDVKKLFQCDECLKWFNSKFLLQRHKLSHMPLEERQWKCTVADCGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCV5</accession>
    <name>T1GCV5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02145051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCV5"/>
    <dbReference type="STRING" id="36166.T1GCV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001128-RA">
        <property type="protein sequence ID" value="MESCA001128-PA"/>
        <property type="gene ID" value="MESCA001128"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982037_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001128-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="284" mass="32248" checksum="E6F5BC813E3C8275" modified="2013-10-16" version="1">MTEFMNITELCRICMNAGVYLLSDQQLDMDGEEINSIDMLKEFSYNEFVEFDTKLRRGEENLLEYLTQSPIPAPQEDEIVSNDEYPDIETIPSVNSEETTDMIIQEQEDLKPINYEVILEVNPPPQKKRKTSTQSEAQSHKNTTKRFHSTAVDITDGETSYMVSAFLPSATVVKAPKACEPPKKSGAPDTSLSKTKVDEVRTIDYSVVRLSGAHDLDFEKHIPAETDTNTVYKCQYCPKAFANSYFLISHVKQVHVCQHCMATFPNRDELNDHTRSAHKSFECA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDC1</accession>
    <name>T1GDC1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDC1"/>
    <dbReference type="STRING" id="36166.T1GDC1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001305-RA">
        <property type="protein sequence ID" value="MESCA001305-PA"/>
        <property type="gene ID" value="MESCA001305"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002902_1_4_1"/>
    <dbReference type="OMA" id="DHVNIIM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000354">
        <property type="entry name" value="ATP-dependent RNA helicase SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040801">
        <property type="entry name" value="Ski2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17911">
        <property type="entry name" value="Ski2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="238"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="478"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001305-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1088" mass="123546" checksum="92F2128694897DD3" modified="2013-10-16" version="1">MKSEESVEILKNYILKPNISIHNPALEIIPRKLKTDKLFEVHGFTPSTKLSANRHPISGEIADFVEVHVEDAGTATSSMSMNRAPDIRNVESTSSVLGSTMNYPFWPAGFEEFASEMEKELNLEDFKLEDNELLTVPPGFSNGHTFSSGNNQISSMKNTVNLLETLEEGLDDVQEWFKIKDEAQNPITTDVILPSRSDSCQWAEMVDISQPVTDFKEKIPEPALTYPFELDIFQKQAILKLEERKYVFVAAHTSAGKTVVAEYAIALSQKHMTRTIYTSPIKALSNQKYQDFKKQFKDVGLITGDLQIDPTASCLIMTTEILRSMLYCGSEITRDIEYVIFDEVHYINDADRGHVWEEVLILLPAHVQIIMLSATVPNTMELAEWVGNTKQQKVYVISTLKRPVPLVHYLYTGCGSKSKNDIFMIVDSEGRFNMKNYQEAKEKKNAMGAKDKAQGNVGRANPKRDQTLWKSMYNNARALQSANLNTGKERGQVQAFFQKCVYKLKPEDRDLPQVQAMKSSLERGIGIHHSGILPILKEIVEILFSNGLVKILFATETFAMGVNMPARTVIFDSHMKFDGNEVRKLKPSEYIQMAGRAGRRGHDENGTVMILCKDDVPSESDLRGIILGQPEKLWSKFLLRYAMILTCLRIESIKVEKIMQCSFKEFYQKSLIPDNKRQLQAAEEKLSALSSLDDSESTKHFGKFYELGMKYIENKKHLMSCLINQPKVNKELKPGKVLVVSKGHHYNKLAILLNIKTGKETIYKNKHFQPEGIGGHTILEIRSSNIIEITKTNLKVEADPIIRNWEQRQMERFKDAPVGQSVAKAVSDLRNLTTNYKKDSKTIEFLNLAQNLNLSVVDEIDMSDINALYALWKEIKQILPYTNIVGFEQSFESVFERKSIEEQITTLKFKSSAKNLSLYPDYLNKLDVLRDLLYINENDEVTLKGKVACEMGQNELLITELILSNVFNDLDSAEIAALLSGLVFQAKVNEKPNVPEKLQKYMDKFTEISDFILNVELRHKVASGESNRLNFGLIEVVYEWARKKPFSEIMGLTQIQEGIIVRCIQQLDETLQDVKTAAMRIASLYTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDD5</accession>
    <name>T1GDD5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02075696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075697">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075698">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02075700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GDD5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001320-RA">
        <property type="protein sequence ID" value="MESCA001320-PA"/>
        <property type="gene ID" value="MESCA001320"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_7_1"/>
    <dbReference type="OMA" id="CEKMSEG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001320-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14178" checksum="FD3222D6B090CFE2" modified="2013-10-16" version="1">MGFILMYDITNEESFNSVQDWVTQIKTYSWDNAQVILVGNKCDMDDERVISFERGKQLADQLGVEFFETSAKENINVKIXXERLVDIICDKMSESLDNDPTLVGAGQKGQRLTDQPQGAPNANCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDK0</accession>
    <name>T1GDK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02040222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02040223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001389-RA">
        <property type="protein sequence ID" value="MESCA001389-PA"/>
        <property type="gene ID" value="MESCA001389"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944368_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001389-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6668" checksum="5BE673A48CA9CB2E" modified="2013-10-16" version="1">MVVSSFAFSNLSIPFSSIVAKDFAISEMYTICPKVRMASLRNCSTQLEQEQVLIGYSIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDR4</accession>
    <name>T1GDR4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02164933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02164934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDR4"/>
    <dbReference type="STRING" id="36166.T1GDR4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001459-RA">
        <property type="protein sequence ID" value="MESCA001459-PA"/>
        <property type="gene ID" value="MESCA001459"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1760875_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001459-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="16524" checksum="2227B50F5BF2DB9B" modified="2013-10-16" version="1">MGPAGKTLTTSQSHCIGGIVGYIDKKVLGEKHIYQHPTAKYTYDSSGFDPEGLFGCIFTVVQSFFNGTVMVHQSNEHMHAPAPLTSRWQALHFYISSHRKLFLEALVIKSFDLLLPIAQRFISKVRKLLFKESSTTNVCNSVSSETDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDT9</accession>
    <name>T1GDT9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02029569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDT9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001489-RA">
        <property type="protein sequence ID" value="MESCA001489-PA"/>
        <property type="gene ID" value="MESCA001489"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1356040_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001489-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="23570" checksum="C6388F75BDBC11B4" modified="2013-10-16" version="1">MISDSSDDEPTLKKPSFLIRLSSYNYRLNHKELKRQHGSKEIKVAVLTKAGDKRLLSLPMPTRDFTVRDLLEAGNIPFNSRDYMHCEDIFSDGNEFNFIVVLLPEGSDKGYADFKVRKFKEKEEKSPTSHCKYCFLLRDEGVKCGRCVKKSNDEENKYIDNEKLLALAKMINDFTVKSEFKEEDKKPKMMKGFSKSEKKRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEQ8</accession>
    <name>T1GEQ8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Regulator of G protein signalling-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02006214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02006217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GEQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001829-RA">
        <property type="protein sequence ID" value="MESCA001829-PA"/>
        <property type="gene ID" value="MESCA001829"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_607339_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015212">
        <property type="entry name" value="RGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45872">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45872:SF2">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09128">
        <property type="entry name" value="RGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Regulator of G protein signalling-like" evidence="2">
        <location>
            <begin position="285"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001829-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="451" mass="51345" checksum="913582150A5C40C8" modified="2013-10-16" version="1">METKYNYFRFGPFYRKPANQSKKDLDSPSRSNNNANNHKRQQRTKSKTKALSDPKMSTQMFLMVEKGENGESELSTPPPLPPRQPGMXXXXXXXXXXXXXPPPLPPRQPGMMEESNLSSNKRNNNARPSPNSIETQWNYPLVTTCQAIRDNGQSNAFPFSQRPNIVQQLKQQSSKTIVFGHDPQSSNNVANNISKVSNDQRKTERTPPGTPPPPYLSQQHQNDDHTDHILPVCTTEDMLIQSETVIPTEIISMEDEELSDQEVVLDVNTPFALLPKLLDNENIVYLAVFLNFLFSNSDPTPLLFYLITDLYKEGNLKELRKWAYEIHSTFLVPSAPLAWYNVDESLINEVDMTLQQDYDKSELMKKVFLKSRKKAKERISEQLRDFQVKRTAGLGTIYGPTDAQLADAKGDKLKEQKIFEETVLPKLQQLLLSSGVKYAYNSTLKATKLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFC4</accession>
    <name>T1GFC4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAM20 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02176705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFC4"/>
    <dbReference type="STRING" id="36166.T1GFC4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002061-RA">
        <property type="protein sequence ID" value="MESCA002061-PA"/>
        <property type="gene ID" value="MESCA002061"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471645_0_0_1"/>
    <dbReference type="OMA" id="CCRIKGS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF22">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN CG31145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM20 C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002061-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9905" checksum="FE03A4259D04FD19" modified="2013-10-16" version="1">MSILAPILQCCMIRQTTLEKLLEFHNGPTPLSQVMRKKMNHDPVAPVLWEPHLAALDRRIEIILQGVRDCLKKNPSDDNSAAEDDVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH22</accession>
    <name>T1GH22_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH22"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002705-RA">
        <property type="protein sequence ID" value="MESCA002705-PA"/>
        <property type="gene ID" value="MESCA002705"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062795_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002705-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="262" mass="30096" checksum="E11F5065B9454B27" modified="2013-10-16" version="1">MNRKIFHYKNAGAESFEKKKDSFVSSNFERSIGNQANFNYEGINSEDMDAPSQDLFENSSSNSKKIKNLLEITKNQEIYDDFGLISNREMDAPLQTLFEDSTSKTAALESSENLTLGQMDENFEEASVVIFVTSYLDMVSKIENLRAIQVNQYSTVEDIKAILARERVENSKILTEMKDMKQDIKILLVVLEWANENTNEDVLGFKLHLSTVEDLNRMEEELEQNLDFKSALLYQHPDVAKKIRSKAGHVEKMEPRDNQLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHI9</accession>
    <name>T1GHI9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02176073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHI9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002891-RA">
        <property type="protein sequence ID" value="MESCA002891-PA"/>
        <property type="gene ID" value="MESCA002891"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1760876_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002891-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="16781" checksum="0DE8EB2F7D7A775E" modified="2013-10-16" version="1">MKGLTLSTFGSCFFQPMEATNVIIVQPKAPADPYSMALLPRTAIHPPRTDITNLNPSLVHHFGLEHRFLVPVVPDIFHQLPKGNTLSQGITQHNQSPRPSPRWINKTAKEPVYPVEFTELNQGKHIKTWNYRIKDFVKSQSPGIYTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHQ7</accession>
    <name>T1GHQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02048100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02048101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002965-RA">
        <property type="protein sequence ID" value="MESCA002965-PA"/>
        <property type="gene ID" value="MESCA002965"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1456023_0_0_1"/>
    <dbReference type="OMA" id="IMININL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002965-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="186" mass="21043" checksum="84A28E4714F4B82C" modified="2013-10-16" version="1">MLIINTLKIIIIIMIMKYTMEKTNLIIMAVDILITKMGLIMININLVNLIIMIILTNQIIISTNLITMIIIILINQACILINQLIMTTPNQVTMITTILIILIIMIIINQAQMITTVLTHLITMIITSLINQASILISQLIMTALNQVTMITTTLISLKMMTIINQVQMIITILTYLIIMIIINRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIG8</accession>
    <name>T1GIG8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein UBASH3A homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIG8"/>
    <dbReference type="STRING" id="36166.T1GIG8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003246-RA">
        <property type="protein sequence ID" value="MESCA003246-PA"/>
        <property type="gene ID" value="MESCA003246"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061640_0_0_1"/>
    <dbReference type="OMA" id="KWAITET"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000032">
        <property type="entry name" value="Blast:Protein UBASH3A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="266" mass="30668" checksum="61F2EC3435FA7D6D" modified="2013-10-16" version="1">MRHGERIDFTFGTWIPYCFDEFNMYHRKDLNMPKSLPNRQNCPDGWQSDSPLTNIGIHQAFLTGEALRDANVKVHHVFCSPSYRCVQTCSSVLKGMNLLDKMEINIEPGFFEWLAWYPEKLPNWLTTSELKDAQFNINGNYKPVFQESSLEDSLKETAEEFYTRNFNVLNEILKNTTGNVLIVAHAATLDTCTRQLIGEPPRSAYELGKIIHKIPYCSIVNIEQTEALKCEINSSNKSNWTLIEPICPSVTHNKNIRFDWNVLLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIT7</accession>
    <name>T1GIT7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02395013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GIT7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003372-RA">
        <property type="protein sequence ID" value="MESCA003372-PA"/>
        <property type="gene ID" value="MESCA003372"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_257065_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005003">
        <property type="term" value="F:ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10319">
        <property type="entry name" value="EphR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000089">
        <property type="entry name" value="Eph receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000005">
        <property type="entry name" value="Eph receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000081">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF265">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0915">Sodium</keyword>
    <keyword evidence="10 19" id="KW-0769">Symport</keyword>
    <keyword evidence="5 19 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 19" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="839"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="24">
        <location>
            <begin position="447"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="734"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1151"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1206"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1250"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1255"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003372-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1360" mass="152863" checksum="8D93D26A4719458D" modified="2013-10-16" version="1">MTESAKNLSSFGNYLNDYELQMTSDKTDSTDKHKKILEIAVKPDYTMERGSWSSKLDFILSVVGLAIGLGNVWRFPYLCYKNGGGAFLIPYFVTLILAGIPMFFMELALGQMLTIGGLGVFKIAPIFKGIGYAAAVMSCWMNVYYIVILAWAIFYFFMSMRKDVPWRTCNNSWNTPACVNPYERQNLFCWNSDETKICALAGQNLTFSDLTDPVKEFWEGITLPGAIEGIKFYVIPNFSKLSQSEVWIDAVTQIFFSYGLGLGTLVALGSYNKFTNNVYKDALIVCSVNSSTSMFAGFVIFSVVGFMAHEQNRPVAEVAASGPGLAFLAYPSAVLQLPGSPLWACLFFFMLLLIGLDSQFCTMEGFITAIIDEWPQLLRRRKEIFIAIVCGLSYLVGLTCISQGGMYVFQILDSYAKKCLANQIIIQKKMKYSIFLTICFLNSTISDQVVLLDTTKEATLEWTRYPYGPQTQTPGWVEESFTNFGRGINWRSYVVCDVAYNNVNNWLWSPFIDRGPANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQPESYKLIARIAAGEGRFNQNSDVDINTEVKSIAVTKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAFFKETPTGKEITLIEKQNGSCVENAEIDEMPPTYLCKGDGKWTILNGGCKCKVGYEPDFEKQSCKEANSLYIVQVYSHSKEDIIGNYSEISFTTEYTISTEILKVYIEKVFENEIYLKWDKPLSSIEFYEVKWFIKGGDIFDMNKTSSLRTKELETVIENLLENTEYGFQVRWKTTNGYGPYSNIIYATTQSGIHTVYDDSAQMRIYAGATIAVVIVLVLIIFATVFFLRTKNHDELEKKTTNHLPLSMDYASNEVNSLDTTPIVKSKANELSAFGHPFPPFSQMSTTDVECFQGKIFYNSDCSAVIIDPHEKHAKTEKKINQSQPPPPIIHTFRVVTASDVEKNVDSQKSIPQKPILMCFICKLSFGNVQIFQQHSNTEHGCHYTTKRTRESPDIVFRTNCDGRHKEDDAATAAATNFLSLAAFSNIFQYSQQKNNLNVCQEHGNVKGNDCKACELSILRTSPSSTTPSFTIGACPDHVNGRPLGVECSRCELILSSSRLTTNSQTMSSTRNSCKTLKCPQCNWHYKYQETLEIHMREKHPDGESACGYCLAGQPHPRLARGESYTCGYKPYRCEICNYSTTTKGNLSIHMQSDKHLNNMQELNTTQNINLTINGPPAPNIQPQVSSVGAQPTAQSPVSSQSSNAKAKPNFRCDVCSYETSVARNLRIHMTSEKHTHNISVLQSNMKHFQTLNLIQQQQITNILPDNFSNNLLPEAAIADMAYNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJH9</accession>
    <name>T1GJH9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02127581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02127582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02127583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJH9"/>
    <dbReference type="STRING" id="36166.T1GJH9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003628-RA">
        <property type="protein sequence ID" value="MESCA003628-PA"/>
        <property type="gene ID" value="MESCA003628"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1096559_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003628-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="254" mass="29149" checksum="AA55EB5F39A7F79E" modified="2013-10-16" version="1">RLHDTYETDRTVTHILELAGGGELVKDNLLKKDYYTEREIAMYIRQLLWGLEHMHDVGYGHMGLTLKDLLISIPNSNILKLCDFGLSRRIHSTRLTTLDYGMPEYVSPEVVNKEGVGFAHDMWGVGIITYVLLSGMNPFRGADDRETLRLIGEGQWDFNDSCWRHIINMLNTRVVQSTVGDNEEISKAYLPIWYSDLVASIRKEMSEGRTTKDLVYRSSKHNRCNEDNSNAAKTATRLLAMKIWSVKWTLNYTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJM9</accession>
    <name>T1GJM9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA hydrolase/transferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02150284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJM9"/>
    <dbReference type="STRING" id="36166.T1GJM9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003679-RA">
        <property type="protein sequence ID" value="MESCA003679-PA"/>
        <property type="gene ID" value="MESCA003679"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030703_0_0_1"/>
    <dbReference type="OMA" id="GCITNSE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21432:SF20">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21432">
        <property type="entry name" value="ACETYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="1">
        <location>
            <begin position="115"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003679-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="27145" checksum="249813A627CC521F" modified="2013-10-16" version="1">MFRSFGDTLIHSSHIDKAVEIDMPLFELPSAPPDKDEKKIGQLIAENLVEDGSTLQIGIGKIPEAVLEALSNHKNLGIHTEMFSNGILPLYEKGCITNSEKRNHRGKIVSSFLIGDKNLYKFVDNNPLIELYGCDYTNSAKVISANPKVVAINSCIEVDLTGQICSDSIGTGFFSGFGGQVDFIRGASEGFDGKGKPIIAFNSATAKGGSKIVPILKPGAGVVTTRAHAHYVVTEYGIASLFGKNMSREHMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJR6</accession>
    <name>T1GJR6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02069871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJR6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003716-RA">
        <property type="protein sequence ID" value="MESCA003716-PA"/>
        <property type="gene ID" value="MESCA003716"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128551_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003716-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="148" mass="17068" checksum="7DFABE6B3BE4A21C" modified="2013-10-16" version="1">MIAINSLRNNFPCHNEVLRMKIYATGFDFYFFLKNDNGTTKYTINISGYNRRFCQLIDDSGTVLAQVDCLKLTSDHIYTEFDLLINFDGTISLFKDGEKEAFMQTTSTDSASISSVSFGRYRKETVDTYLYFDCPFECNNLDENIIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJZ3</accession>
    <name>T1GJZ3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02131977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02131980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJZ3"/>
    <dbReference type="STRING" id="36166.T1GJZ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003803-RA">
        <property type="protein sequence ID" value="MESCA003803-PA"/>
        <property type="gene ID" value="MESCA003803"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008532_0_0_1"/>
    <dbReference type="OMA" id="DHAGQQV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22405">
        <property type="entry name" value="KH-I_Vigilin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22413">
        <property type="entry name" value="KH-I_Vigilin_rpt10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22414">
        <property type="entry name" value="KH-I_Vigilin_rpt11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22416">
        <property type="entry name" value="KH-I_Vigilin_rpt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22417">
        <property type="entry name" value="KH-I_Vigilin_rpt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22418">
        <property type="entry name" value="KH-I_Vigilin_rpt15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22406">
        <property type="entry name" value="KH-I_Vigilin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22407">
        <property type="entry name" value="KH-I_Vigilin_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02394">
        <property type="entry name" value="KH-I_Vigilin_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22410">
        <property type="entry name" value="KH-I_Vigilin_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22411">
        <property type="entry name" value="KH-I_Vigilin_rpt8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000039">
        <property type="entry name" value="vigilin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF31">
        <property type="entry name" value="DODECA-SATELLITE-BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="60"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="137"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="209"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="320"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="390"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="461"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="535"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="605"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="663"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="736"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="809"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="897"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="975"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="1048"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1158"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="948"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1179"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003803-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1198" mass="133679" checksum="57F48B670B790ECB" modified="2013-10-16" version="1">MEDSSLNDVQQQSQENHGGAGQPAFSYDDLFPALPANAAPKASAGPTTVKQNHQMRVGTSVITQVFHVPCEERKTSSDSETNFGEGENLRTCQSIMKETGAHIEISSGKDHSLTFLVKGKPNEVLEARRKILTDFQTQASKQISIPKEHHRVILGKKAERLRELEKVTATKITVPNQTDESDIITISGTKEGIEKAEYEIRSMSNEQLKKAVERINVPRKYHPFISGPFNETVSQLMSETGAKIHIPPASVNKDEIMITGEKDAVVTARNKIEVIYKDMEKKCATVSVEVANSPSDTITLRGPQIALGHALTVVYQKANSVKSLDLEAPVWIHKYIIGKKGASIKEFEANNPNVNLDFTAEDKIKIEGEPDKVDQAAAHLREIVQNYVKNYSFVIIPVNPNHYKHIIGKSGANINRLKDDLKINVSFDTRDGDNHIRIEGPKEGVLQAQRELQEKIDKLENEKSKDVIIDRRLHRSIIGPRGEKIREFKERYGQVNIAIPSPSENTDIIKIRGPKEDVDKCHKDLLKLVKELQENSFVVDVPIFKQFHKYIIGKSGANIKKIRDETQTKIDLPAEGDANNEVIVITGKKENVLEAKERIQYHNSLIGGGGKLISAIMEECGGVTIKIPSSDSKSDKIIIRGPGEDVLKAKEQLLELASEAQLASFTAEVRAKQQHHKFLIGKNGASIRKIREATGARIIFPNNNDEDKEVITIIGKEESVKLAKEQLETIIADIDKVTDGEISVDPKYHKHFLTKRGEILQRISDECGGVLISIPPMETNSDRVTLKGAKECIEAAKKRIQDIVNDLEAQVSVEVIIPQAHHGNIMGQRGSRIQQITSKYDVQIKIPDRNATELVPIVNGESGEEPTTARQCDIITITGTIEKCNAAKQVLLDLIEITEEVHVPFEMHRSIIGQGGRSVKELINRYEVRIELSPPDSKLDIIKVTGIKTNVSEAKEALLKKVEEFEAEKKDRELRSFEVKVDIDPDYHSKIIGRRGAVVNKLRADFDVNISFPKAGDEDNRTVTITGYEKNANECKEEILKMISDYNDYIKEVVNIDARVHPRLIGNRGRNIKKIMEDYKVDIKFPKATDEDKNAVTIIGHEENVENVKDHLLNLEEEYLQDVIDVAPSHRNQSSDLSAAFEQAFNQTGFVVKGAPWERQKGSAPNTQSQEDFPDFGGKTNSNSETAPISSAWGGSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK39</accession>
    <name>T1GK39_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK39"/>
    <dbReference type="STRING" id="36166.T1GK39"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003854-RA">
        <property type="protein sequence ID" value="MESCA003854-PA"/>
        <property type="gene ID" value="MESCA003854"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089077_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="14"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="139"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003854-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="27606" checksum="D2639D56D1BB33DF" modified="2013-10-16" version="1">MSSISGILSQSDIDEICLQRADEGLDYLAHPNDCSSYIICDNPASLGSCEEFYFNEEAQTCDYKENVQCDNVEPEPQPEPEPETEPPTEAQTTIITTTTETTTTSTTAELVPQTTLPTTKEVEIETTLSSASTTSPLESVLCPEVSHEIVFKADPDSCLNYYLCFNGVAMPMKCADNLHFNSEKQKCDFKENVRCRLGIPTCEIYMNEFFPHESSCNLFYYCRYGHLVSSNVHTFIIGMRKNMNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKA2</accession>
    <name>T1GKA2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GPI inositol-deacylase PGAP1-like alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKA2"/>
    <dbReference type="STRING" id="36166.T1GKA2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003921-RA">
        <property type="protein sequence ID" value="MESCA003921-PA"/>
        <property type="gene ID" value="MESCA003921"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2136316_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050185">
        <property type="term" value="F:phosphatidylinositol deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006505">
        <property type="term" value="P:GPI anchor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039529">
        <property type="entry name" value="PGAP1/BST1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495:SF7">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495">
        <property type="entry name" value="NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576987" description="GPI inositol-deacylase PGAP1-like alpha/beta domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="7">
        <location>
            <begin position="56"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003921-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="13161" checksum="A5E0F6DC5418A831" modified="2013-10-16" version="1">MFHWPTFFGILYQLFLTLHPCPLMRIHFDENKKFPKYGLYAYSEGKLTEKARAMEFTGAPVVFVPGNSGSYKQARSFASVAIRKGLDNNWNHHLDYFTVLVVVDKMVDFDMEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKD3</accession>
    <name>T1GKD3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003956-RA">
        <property type="protein sequence ID" value="MESCA003956-PA"/>
        <property type="gene ID" value="MESCA003956"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815360_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003956-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7975" checksum="67BC4B0F31B2103B" modified="2013-10-16" version="1">MEVFGQSSKFTIQMLVPKQAYWWQLKEYINIFYWFCTHDRKTLVPCLFFFISLGNEEQTSDLAAVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKH2</accession>
    <name>T1GKH2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02018059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02018061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKH2"/>
    <dbReference type="STRING" id="36166.T1GKH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003995-RA">
        <property type="protein sequence ID" value="MESCA003995-PA"/>
        <property type="gene ID" value="MESCA003995"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_895138_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="17"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003995-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="311" mass="35554" checksum="B729A11915B6602E" modified="2013-10-16" version="1">MQDTIYRLVHLADIRISIQTLSLLLQLVGKKSVKSDRFYNALYKKMLDLNLTNIGAVTAANFLHILHKAIYIDSNIPRCLAFIKRLLQLALYVPAHVAAGCLIIINKLFKSRRELVKTLDEPETTIAPVEVDLSKFDDDSDGEEIYKDVDDEKNNVDSTKKEKVSSSWHHVKVQSVEVPESNVKEIAGNKYDPYHRVPAFAGADNSLRAELLLLCQHFHPTVQVFAESVLNQKKISYYGDPLKDFSLAHFLERFVFKNPKKNEKSLSEVPQTVQHKNYISYGSRGKSVKSLTRTNCTEDEKFIFNIWKRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKX6</accession>
    <name>T1GKX6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKX6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004159-RA">
        <property type="protein sequence ID" value="MESCA004159-PA"/>
        <property type="gene ID" value="MESCA004159"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_651476_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040985">
        <property type="entry name" value="Nup54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF6">
        <property type="entry name" value="CHK DOMAIN OV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18437">
        <property type="entry name" value="Nup54_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="44"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004159-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="422" mass="48445" checksum="962E0F5FA1037CBB" modified="2013-10-16" version="1">GSNKTINYLLKIGHGSNATGSEKVDALGHLKGRMFSENPGVDNIVLEDLKIRNFKNADRLKGLDMEHCQSVLKLLAEFHAASAVYFELNGPYPDIITIGIFREDLLPMFVEFNLGIQSILKPLMSTLKDYENGKYYADKLFRKDYETYYKEGHKHVSQINSGSFNVLNHGDIWSNNIMFSYNDQNELQDTCFVDFQMSKYGSPFYDLYYFLLSSVALDIKLEKFDYFIKFYHDNLIESLRLLEYPKTYPTLQNLHEMLLQNGFAAVMTTVGLMGTVLLDPTEQESMGNFFSDEEGAIAFRKMIYTNPKYIIVKQECTRKVGMALTPEEEALRSKLENMQDLISAPTQFKGRLSELLSQMRMQRNQYALTGANEYTIDKDSEDEMKSFLTMQQKAMEVLIETINKDLKALAIITKGMPELMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLX0</accession>
    <name>T1GLX0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Interacts with melt.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02027662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02027663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLX0"/>
    <dbReference type="STRING" id="36166.T1GLX0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004535-RA">
        <property type="protein sequence ID" value="MESCA004535-PA"/>
        <property type="gene ID" value="MESCA004535"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530068_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767:SF2">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="15">
        <location>
            <begin position="36"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="13">
        <location>
            <begin position="36"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004535-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9607" checksum="5AADD11155EABB24" modified="2013-10-16" version="1">MKEIYIKETISPPDPQQTTPQGINSSKKNSSRRNAWGNFSYADLITHAITSAPDKRLTLSQIYEWMVQNVDYFRNKASPCQVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLX4</accession>
    <name>T1GLX4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02183719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLX4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004540-RA">
        <property type="protein sequence ID" value="MESCA004540-PA"/>
        <property type="gene ID" value="MESCA004540"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3175942_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="47" mass="5481" checksum="F9666027AAC71BBA" modified="2013-10-16" version="1">MLLKAQKKTGLRLSKKTRPKWCLLKVELKGWTSLSPRNASPMSQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLY2</accession>
    <name>T1GLY2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02187444">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLY2"/>
    <dbReference type="STRING" id="36166.T1GLY2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004548-RA">
        <property type="protein sequence ID" value="MESCA004548-PA"/>
        <property type="gene ID" value="MESCA004548"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297247_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000047">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="10">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004548-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="213" mass="25214" checksum="603C955A76FCE4E4" modified="2013-10-16" version="1">MSLDIRTIFVQTCLPLKTTLIILNMISNIDNITSEHCLIMQLNIRIFQYSLKIRIVIFTFTNIFLFLSNFRLHTVQSNMSTSRRRKPEKAGRGLRHFSMRVCEKVKEKGTTTYNEVADELVKEEFKEDSANCDQKNIRRRVYDALNVLMAINIISKDKKEIRWLGLPTNPTEQYNDLIDENRQRRENCEAKQQQIEELLLQLISFKKLIERNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLZ5</accession>
    <name>T1GLZ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLZ5"/>
    <dbReference type="STRING" id="36166.T1GLZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004561-RA">
        <property type="protein sequence ID" value="MESCA004561-PA"/>
        <property type="gene ID" value="MESCA004561"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006383_1_1_1"/>
    <dbReference type="OMA" id="ANCNDAI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="5">
        <location>
            <begin position="91"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="254"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004561-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="366" mass="41872" checksum="7BD92C56007E4D9D" modified="2013-10-16" version="1">MSSTNTTSNVANCNDAIRLLLPPLHCPNDTEQLQNPTKPFPIRGERAINDVNVPHVIAMVGLPARGKTFISKKLSRYLNWIGISTRVFNLDNEEAMTIRNKCAHQALDDACQWLLSESGGSVAVFDATNSTIERRQLIHDIVVNRHKLKLFFIESICDDPKIVDQNIKEVKVSSPDYVNMNTDLVLNDFLKRIEHYQERYQTLDEVQESYLSFIKIYNTGTKVVVFNNEGHLQARIVYYLMNIHITPRTIYLTRHGESEHNLKGLIGGDSDLSNRGKLFAKSLSNFINEEQILGLRVWTSCMKRAIQTVDDIDAPKENWRALNEIDAGICEEMKYTKLKLNIRKNLKLEMRINSHTDIQGARAMRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM52</accession>
    <name>T1GM52_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent RNA helicase DHX8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02182379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM52"/>
    <dbReference type="STRING" id="36166.T1GM52"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004620-RA">
        <property type="protein sequence ID" value="MESCA004620-PA"/>
        <property type="gene ID" value="MESCA004620"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_2_1_1"/>
    <dbReference type="OMA" id="MKEVDQV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17971">
        <property type="entry name" value="DEXHc_DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21691">
        <property type="entry name" value="GH2-like_DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05684">
        <property type="entry name" value="S1_DHX8_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000061">
        <property type="entry name" value="ATP-dependent RNA helicase DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000101">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000191">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044762">
        <property type="entry name" value="DHX8/Prp22_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049588">
        <property type="entry name" value="DHX8_GH2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049621">
        <property type="entry name" value="S1_DHX8_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="271"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="578"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="759"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="224"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="341"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="500"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="225"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004620-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1199" mass="136596" checksum="7B0903E024E33A87" modified="2013-10-16" version="1">MDELKQLEYLSLVSKICTELDNHLGINDKDLAEFIIHLADENPALTDFRRALESNGAEFPDSLTENLLRIIKLMRSSSGKSSDVDTKTYDTKSEKLANLFPGLAIPNEKKAKEESDDEVADAMAELEALAPNKDEPSTSKTEKRRHSPERRRERSSSREKSKRHRSRSRDRNKRGRSRSRDRAGKEEADPRIGKEGAVPGEIHWINIAYKILKIPLERINVLSRDRKRRSRSRDRKGRRDRSREHKRSRSRDRDRHRRRSPTPMPEDPEVGKIYSGKVANIVQFGCFVQLLGLKKRWEGLVHISQLRAEGRVSDVTEVVSRGQSVKVKVMSIAGQKVSLSMKEVDQNSGRDLNPLSHSHLNDDAKDRNPDRPVGGTSRLHLTIDGQETIEEDETRKRVTRISSPERWEIKQMISSGVIDRSEMPDFDEETGLLPKDENDEEDIEIEIVEEEPAFLRGHGRALNDLSPVRIVKNPDGSLAQAAMMQSALQKERREQKMIAREAENESNQSGTNKNWSDPLPDEEKPSTMRGVGMSTAEIPEWKKAVIGGKKSSFGRKTDLSIVEQKQSLPIYKLRDDLIKAVTDNQILIVIGETGSGKTTQITQYLAECGFQARGKIGCTQPRRVAAMSVAKRVAEEFGCRLGQEVGYTIRFEDCTSPETFIKYMTEGMLLRECLIDLDLKNYSVIMLDEAHERTINTDVLFGLLKQAVLKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEVLYTKEPETDYLDASLITVMQIHLREPPGDILLFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPAGSRKCIIATNIAETSLTIDGIFYVVDPGFVKQKVYNSKTAMDSLIVTPISQAAAKQRAGRAGRTGPGKAYRLYTERAYRDEMLPTPVPEIQRTNLATTVLQLKTMGINDLLHFVSWMLLPLGRRMAEFPLDPNLSKMLIMSVALQCSDEILTIVSILSVQNLFYRPKDKQALADSKKAKFNQTEGDHLTLLAVYNSWKNNKFANAWCYENFVQIRTLKRSQDVRKQLLGTMDRHKLDVVSAGKNSVRVQKAICSGFFRNAAKKDPQEGYRTLVDQQVVYIHPSSALFHRQPEWVVYHELVQTTKEYMREVTAIDPKWLVEFAPSFFRFSDPTKLSKFKKNQRLEPLYNKYEEPNAWRISRVRRRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM71</accession>
    <name>T1GM71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM71"/>
    <dbReference type="STRING" id="36166.T1GM71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004639-RA">
        <property type="protein sequence ID" value="MESCA004639-PA"/>
        <property type="gene ID" value="MESCA004639"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075840_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004639-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="13089" checksum="99DBA755A998EB7F" modified="2013-10-16" version="1">MSLVSGYGTHLFPTFHPHLLQSLNHGMLSNNYFSNDRNKPVLGNFQLPSAFSPPKYIGISLEQNILNSSNESFRTDSASPTCTSHESIEGSNDYDGEKGESPRSNNSDPRDLRPFILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM95</accession>
    <name>T1GM95_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390002">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM95"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004664-RA">
        <property type="protein sequence ID" value="MESCA004664-PA"/>
        <property type="gene ID" value="MESCA004664"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963409_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004664-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6473" checksum="A7C4A24AB30A195C" modified="2013-10-16" version="1">MLAGISSIVCKIINSIIKREVYWNTENTCHTGLFIIETCCVYSGVGAAKVYLPDDSFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMD7</accession>
    <name>T1GMD7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMD7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004709-RA">
        <property type="protein sequence ID" value="MESCA004709-PA"/>
        <property type="gene ID" value="MESCA004709"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519024_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004709-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9747" checksum="D27769764A8D6A76" modified="2013-10-16" version="1">CLGNRPIGDWVDFEWGDRLPQNAVKGGYDQTTSLFICRAFNHTLSMKYCGKTSKFGRCWVDHKGKEKAIKKFQILTGVYGFWVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMJ4</accession>
    <name>T1GMJ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02115321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMJ4"/>
    <dbReference type="STRING" id="36166.T1GMJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004769-RA">
        <property type="protein sequence ID" value="MESCA004769-PA"/>
        <property type="gene ID" value="MESCA004769"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_843540_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF13">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004769-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="35735" checksum="5C5C52CD84A3A370" modified="2013-10-16" version="1">MGRIAGLASGLDTGETPIAKEIHHFIHLITGVAVFLGVTFFAIAFILGYHWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMASKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIIEADTTEDQSGVQYDRTSPGFKALSRIATLCNRAEFKGGQEGVPILKKEVSGDASEAALLKCMELALGDVMSMRKKNKKIAEIPFNSTNKYQVSIHETEDSNDPRYLLVMKGAPERILERCGSIFINGKEKVCNEILRYHTEIVFARTSPQQKLIIVEGCQRMGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMP4</accession>
    <name>T1GMP4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02131580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMP4"/>
    <dbReference type="STRING" id="36166.T1GMP4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004822-RA">
        <property type="protein sequence ID" value="MESCA004822-PA"/>
        <property type="gene ID" value="MESCA004822"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027239_1_1_1"/>
    <dbReference type="OMA" id="XHASNIL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004822-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="26121" checksum="70B569A7B9F18532" modified="2013-10-16" version="1">MCKNLSRPRLIKSHADIKFLPKQLWTKNPKIMFVVRDPRDAFVSLYHHNKFFFGKTVEESIESFIQDKLKYGNFWPMVLSFYALRHRSNIIFFSYEEMKRNLELVVLKVAKFIGRDYDEGQIRELLEHLDFNSMKSNVVCSHRLDLEEVRKKKNIQKDDIDENACFYRKGKVGSYKDELSEEILNKIDKWQKNFLDCEGIYLEDILWSNGHVEHSEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNH2</accession>
    <name>T1GNH2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02001400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005123-RA">
        <property type="protein sequence ID" value="MESCA005123-PA"/>
        <property type="gene ID" value="MESCA005123"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_964078_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005123-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="289" mass="32661" checksum="A17FC07BE45B6FB5" modified="2013-10-16" version="1">MCENVLQQSHLENGDNTFADPIWNCLDNPLQTRRMLKNRINSNKKLLPFEAKPKPEVYKQPKVSLGLNLQPSKNRKESRPSLEIQHKNKKPRLDFTTQINETITQSDLDLKAQSPLELDVENLSPKASEIPIKVGGCPGTPPRTPKRRSSINPLAKKAIIQNAEQYTPGKQAKTPPQSIHELDERLKSLNRKTKSILNSPLKNGPKEEEHQFLEQEAIEEQHVDQGVNEDQHLNQGVKTVSIIEVLDSSDENNEEIPDTQHPKSISEGGQLDEERGKTEEDNHESHSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNI3</accession>
    <name>T1GNI3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-alpha-acetyltransferase 20</fullName>
            <ecNumber evidence="5">2.3.1.254</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionine N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">N-acetyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N-terminal acetyltransferase B complex catalytic subunit NAA20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">N-terminal acetyltransferase B complex catalytic subunit NAT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NatB catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-terminal L-methionyl-L-asparaginyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-asparaginyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50484"/>
            <dbReference type="Rhea" id="RHEA-COMP:12694"/>
            <dbReference type="Rhea" id="RHEA-COMP:12695"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133356"/>
            <dbReference type="ChEBI" id="CHEBI:133358"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-terminal L-methionyl-L-aspartyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-aspartyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50480"/>
            <dbReference type="Rhea" id="RHEA-COMP:12692"/>
            <dbReference type="Rhea" id="RHEA-COMP:12693"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133045"/>
            <dbReference type="ChEBI" id="CHEBI:133063"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N-terminal L-methionyl-L-glutaminyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50492"/>
            <dbReference type="Rhea" id="RHEA-COMP:12698"/>
            <dbReference type="Rhea" id="RHEA-COMP:12699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133361"/>
            <dbReference type="ChEBI" id="CHEBI:133362"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-terminal L-methionyl-L-glutamyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50488"/>
            <dbReference type="Rhea" id="RHEA-COMP:12696"/>
            <dbReference type="Rhea" id="RHEA-COMP:12697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133359"/>
            <dbReference type="ChEBI" id="CHEBI:133360"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the N-terminal acetyltransferase B (NatB) complex which is composed of NAA20 and NAA25.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.254" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02108216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02108217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GNI3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005136-RA">
        <property type="protein sequence ID" value="MESCA005136-PA"/>
        <property type="gene ID" value="MESCA005136"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187912_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005136-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="91" mass="10683" checksum="453C058150E8FF21" modified="2013-10-16" version="1">MNFLEDVSEKKKAYFVDLFVRVSNTVAINMYKNLGYIVYRTILDYYFGDPDEDAYDMRKACSRDVXXXXXXXXXXXXXXXXXXEKSLLKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPM7</accession>
    <name>T1GPM7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02002348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005561-RA">
        <property type="protein sequence ID" value="MESCA005561-PA"/>
        <property type="gene ID" value="MESCA005561"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239687_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005561-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="12261" checksum="89D34CF754C49585" modified="2013-10-16" version="1">MSVIFLHDVVKLRTLKFVIAQLRAFVQEARFIQSQLKDKPLERALRDAILMVTNSSQTRQNILKKKDPILKEVLDIAKERELVLSESNNMDKTKELHSKQTLFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ35</accession>
    <name>T1GQ35_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02016103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ35"/>
    <dbReference type="STRING" id="36166.T1GQ35"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005734-RA">
        <property type="protein sequence ID" value="MESCA005734-PA"/>
        <property type="gene ID" value="MESCA005734"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1316744_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051952">
        <property type="entry name" value="Golgi-autophagy_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157:SF25">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="4"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="105"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005734-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="209" mass="24572" checksum="C20D7181C91BF03C" modified="2013-10-16" version="1">MVMLRELQKLLSDERHLKESLEMQLNELKNQISSNSIHDDNYNQLLSELKEARSKIRKISMQKSEHSENTENDSILQQLQGELQNMKHQHSIALKHEQMKREKLAILHEDRVANLESRLAELSNTVGSYDRLRQQDQDNISKLKEKLSKLNMNQSEVIPDMKHEVDIPKIVEEIFKLKKIIQTENENLQNPFDLNNYKVAKCLPIIKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ56</accession>
    <name>T1GQ56_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Laminin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ56"/>
    <dbReference type="STRING" id="36166.T1GQ56"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005757-RA">
        <property type="protein sequence ID" value="MESCA005757-PA"/>
        <property type="gene ID" value="MESCA005757"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018213_4_1_1"/>
    <dbReference type="OMA" id="CENTSPR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF365">
        <property type="entry name" value="NETRIN-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577595" description="Laminin N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="4">
        <location>
            <begin position="39"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005757-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="330" mass="37216" checksum="F07A6F25CA9A22F0" modified="2013-10-16" version="1">MKKLVILLGLLQLVNCGEKYLRLYAPPRSTDPCYTDEGKAQKCIPDFVNAAYGAPVTASSTCGQRGPEKFCDLDNPSQCYTCENTSPRRKYSSTALTDLNNPNNVTCWRSEILQPSKSAAINLNSNSNSNSIQSSPLSQSSLTLNTKIHLDNITLTLSLGKAYELTYVSLQFCPKAIKPDSLAILKSMDFGRTWQPFQYYSSQCRQIYGRPNRETIQKSNEQEARCTDSHKYDGGSNMARGSRIAFSTLEGRPSARDFDSSKSLQEWVTATDIRVIFHRLQMPQDVAAYDDDDDGKMRTTMRRMKRIVQKMMTMICCTRIIARRITMIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ72</accession>
    <name>T1GQ72_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02147184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02147185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ72"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005773-RA">
        <property type="protein sequence ID" value="MESCA005773-PA"/>
        <property type="gene ID" value="MESCA005773"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1911907_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005773-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="14688" checksum="5150D2A1EE855598" modified="2013-10-16" version="1">IVFFTALFYLLASSDTKYAPLQIGTYFGFSSGNKLGDALENSISGVLLSMFKCCIFQGLFTWLVHTVLGARVVFLPSALAAILSAAPFLGSYWCALPAFLELWTIHTVQQKMMLTIKHLAQMSTWVVIHQCST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS2</accession>
    <name>T1GQS2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02109107">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02109108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005986-RA">
        <property type="protein sequence ID" value="MESCA005986-PA"/>
        <property type="gene ID" value="MESCA005986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3421490_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="24" mass="2967" checksum="52C30FA7D1183790" modified="2013-10-16" version="1">MLDLRNSQRKSNSFKVRCKIYRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQT5</accession>
    <name>T1GQT5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02104174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQT5"/>
    <dbReference type="STRING" id="36166.T1GQT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006000-RA">
        <property type="protein sequence ID" value="MESCA006000-PA"/>
        <property type="gene ID" value="MESCA006000"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015987_0_0_1"/>
    <dbReference type="OMA" id="YEAHAAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006000-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="62419" checksum="458E391B18FE7FD8" modified="2013-10-16" version="1">MAHCPIYTPDLLNICSSVQPLEVKLQNTPFPSQQNLSYDSSDYAEIDEICLELHSTKTQTNSNAAKKPYKNGDENEITSSNNLKAVATTSDMGQNLSLRRPRISLTWILREQHTPSNHNNNNTTLNNEHKNGGSGEEKLFSEKYVSNHNLQEFQEKREPSKELLFVCTPSQKPQHHHHKPHHANTTESLIKRRNFEKNSKLQQQHQQLHHHHIHSHHQTQNPLKSSENNVLDKLEKLDETKSLKSTYSEPSLVAASETNHTNSSHHRRHRHRRRRGDRNRMQKFGYEIRNIDEFLTKCSLSSPGNIPVVLATASTLYQTRPGGYQIEISLPLGMVVNAVFKNQNWLYVQTPHAQEGYVVYSACMPLGILPPTVKGNGSLQTTPCWENTGDVFPMPCGNMTDSEKEVQLRGGTRSEGARTPRPKKYYHQPKPATTYSQSAKLAEKAYAQLRTIQMCTEQKAQEAMKLQQQKTKEKTAVHHKRFSKGLRQTLVAITNNYTSENLTVQKGDIVTLVECRESKDHKQWFYVKTRDDREGFIPAEVAGHGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRD1</accession>
    <name>T1GRD1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006211-RA">
        <property type="protein sequence ID" value="MESCA006211-PA"/>
        <property type="gene ID" value="MESCA006211"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2906682_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006211-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="62" mass="7055" checksum="92C2A9563A4E8769" modified="2013-10-16" version="1">MSGGAAVRVNISVQTPQSTRKISLSRPSSPKKFLVDRRSTRIMTVALKFRDYKFCVARRNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRJ9</accession>
    <name>T1GRJ9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRJ9"/>
    <dbReference type="STRING" id="36166.T1GRJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006284-RA">
        <property type="protein sequence ID" value="MESCA006284-PA"/>
        <property type="gene ID" value="MESCA006284"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_537805_0_0_1"/>
    <dbReference type="OMA" id="CERYKND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070129">
        <property type="term" value="P:regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033490">
        <property type="entry name" value="LRP130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46669">
        <property type="entry name" value="LEUCINE-RICH PPR MOTIF-CONTAINING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46669:SF1">
        <property type="entry name" value="LEUCINE-RICH PPR MOTIF-CONTAINING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="PPR" evidence="1">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="58336" checksum="6768F1F3A41AD8FB" modified="2013-10-16" version="1">MFKNQLVASLVKTEDVLSFVKILKNICLNIKSIDAAKEFVGEMTLDALNQLKTKSSHTIPLLLESLLHHNLGISEKFTEIIQSKLEHKVDENISNLDGFDIPNGSKAQLIELYCNSNELDKALNLYEKENETKEFRLDQLKTIKLVDLVLKSGDTEKAINILIENRGKTINEEESYLYKNNIWKLLNNLSETGNVEVLNKLSCRFIQNEDAESLQKLTDLSTTIHGEVNSLYDLVFSFIECGRIRQARQILETPGLKNRSNRISNACERYKNDGNIKSLEAMIEATRDINYIDRNFIYFNLLQSYEKENDHQKALNLWTRMQEENIVPSDTFLIKLGKLLKENNINVPFTIPSSSKKSENIEKSTKLKIVNDPKLKTIDLYNKFEGLLKENKISEASIMFNNFINKTNNIPRRLLKFYINKLASIGDYESIEKMVLSDEIKKKISFDNRLCHAYIKADKVNDYFIKLREDLENCKSIGEIQDKGAAFPRGGALGILENFPDMYEECK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRL6</accession>
    <name>T1GRL6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="8">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CAQQ02082098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRL6"/>
    <dbReference type="STRING" id="36166.T1GRL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006302-RA">
        <property type="protein sequence ID" value="MESCA006302-PA"/>
        <property type="gene ID" value="MESCA006302"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006586_4_2_1"/>
    <dbReference type="OMA" id="IICTTHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147043" description="Carboxylic ester hydrolase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="9">
        <location>
            <begin position="27"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006302-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="26836" checksum="39488F9E5718E603" modified="2013-10-16" version="1">MHPSGSLLTVIFLFQAYGALTSNRSLIIKLNHGGSLKGIELKTETGRSIRSFLGIPYAKAPVGELRFKSPIPYPGWEGQRSATEDGSSCTQAFALLADEGSEDCLFVNVYTPPLEHITNSGLSVMVWYHGGAWFAGSNNHLFYGPEYILEHDIILVTVNYRLGALGFLSSETLDCPGNFGLKDQVEALRWIKEHISSFGGNPNNVTIFGESAGGASVSYLLQSDKPKGLFHKAIQQSGTIFNFWAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRN6</accession>
    <name>T1GRN6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02057886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRN6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006323-RA">
        <property type="protein sequence ID" value="MESCA006323-PA"/>
        <property type="gene ID" value="MESCA006323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1462914_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="185" mass="20851" checksum="CEEDC009C85680D7" modified="2013-10-16" version="1">MVPDEDLSALAASVQKEKEGQEDEVKQEHLVDNDDKEPVPSEPPQEQSTPVPIIVKRGRGRPRKYPRNRNVIHPVKLAKVMKSEGSSMEDGEAKESNHLNKTEPQVILTEKNSDGSSLNKTDPGPAKRGRGRPRKDQSLNSLTPRKPSYSLQMISKKTEKTYINFLDRVPIYQSPRLDYGVDPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRV1</accession>
    <name>T1GRV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02133778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02133779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006394-RA">
        <property type="protein sequence ID" value="MESCA006394-PA"/>
        <property type="gene ID" value="MESCA006394"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266812_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006394-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="12145" checksum="E4CA6A2C74A95E33" modified="2013-10-16" version="1">MFESMTNFVKMKCIPSHCRKRSTQAKLSTFHNAFRERGTDQRFSRLENGKRNTDHGKNGKRKNILLSTLNQAHDSLWEISTSKRKELFEMFLQKSTELPEEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS54</accession>
    <name>T1GS54_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS54"/>
    <dbReference type="STRING" id="36166.T1GS54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006502-RA">
        <property type="protein sequence ID" value="MESCA006502-PA"/>
        <property type="gene ID" value="MESCA006502"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1121196_0_0_1"/>
    <dbReference type="OMA" id="RDECDYT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006502-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="248" mass="27650" checksum="338AA82E7930AC92" modified="2013-10-16" version="1">MKKNSARRGNVFTSGASRIMIRGSPVEAINTLSKLGYKVVCSTGEVECVWTMQQSHSNLHQHCKCILNPTVGSVHQTLSEMDFERGIWNAVIYNDVKKVKEFIENGKAMDKDNTGYTALHYASRSGNEEICKMLLAAKADVNAATNGGCTSLHRASTQGHLHIVKLLLNNKANVLLQDDDGQSALHRAATKNNYQICQAILGNAPDPRFLREDFRKRFLHYVLSISQLYEKSIDCLNIVLITLFFAYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSI0</accession>
    <name>T1GSI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02094850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSI0"/>
    <dbReference type="STRING" id="36166.T1GSI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006640-RA">
        <property type="protein sequence ID" value="MESCA006640-PA"/>
        <property type="gene ID" value="MESCA006640"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_7_1"/>
    <dbReference type="OMA" id="CGINALM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF33">
        <property type="entry name" value="AT22075P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588508" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006640-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="175" mass="18860" checksum="97B2C00FA2DFC957" modified="2013-10-16" version="1">MLIGLVLMALNQFCGCFAMINYTGTIFEQAGSSLSPNMSAIIVAFIQLVGSYASTALVEKAGRKILYLTSCVGTGVGLSILGLYINLSSSGYDCSSFKWVPIAAFSVTIFFSTLALLTLCFLVIAEILPPKIRALGSNICMSVLWIFCFLILKFLPFLADVLGLHGLMLLFASVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSK9</accession>
    <name>T1GSK9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02048756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSK9"/>
    <dbReference type="STRING" id="36166.T1GSK9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006669-RA">
        <property type="protein sequence ID" value="MESCA006669-PA"/>
        <property type="gene ID" value="MESCA006669"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847398_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="13">
        <location>
            <begin position="48"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006669-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15580" checksum="4F59048BCB2E803D" modified="2013-10-16" version="1">MISLYFHRARAQVIDYSVPYFHSGLSLLAAPQSKSEIPLLAFLLPFSPELWIAIFTSLNVTAIAVAIYEWLSPFGLNPWGRQRSKNFSMSSALWVMWGLLCGHLVAFKAPKSWPNKFLINVWGGSALFSLLPTQPTLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSQ6</accession>
    <name>T1GSQ6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02081911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSQ6"/>
    <dbReference type="STRING" id="36166.T1GSQ6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006717-RA">
        <property type="protein sequence ID" value="MESCA006717-PA"/>
        <property type="gene ID" value="MESCA006717"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512210_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006717-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="532" mass="60002" checksum="64118752737BA465" modified="2013-10-16" version="1">MTQSYLNNKNEDLKIPSDWEMFPVKNCKTGDQITPVWTQLLTTFDNESLENVSSVFLKNIEDKENGTSVEMYIQELFKVPNVLVLKDTVGFLSISSNCNSLVEHLAKDLTSFEPETMSDSELENLKAVCSKKEKRINEKNFSSFKKNIIKCFQKHARLNELKEGLTAKDDDFVIIPTVWSLNPEKLTDHQKEKIATKSRDIPALYNDMSQSEDGANFKAWTPKKLVLAKNQEHEIVIEGKDSEEEQSEKESQDRDKSPPRSMQTEKLSFATPSQPLAPPSTPITSSAKSKQSKQFTGRGAQLMNILRSKQGNQHSSSPGSAHVATASPSTGDKDTSSCASPVVNRKLNRLNSPRIPSTPVQPDTPSSILKRKFMDCSFADQTPEQQAKKKRVSFHDPPVSATKEFVTEIVENNNNIEECSSPRVLASGKHSLKRKSRLDSMKEITNLENSETNIQPQPQTQPQSVTPPAETQIEKLTFDTEEHALEHILKKYTCEELLELKIKQLNKEKQSEIVQNPTTTTTLINLIRKNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTS3</accession>
    <name>T1GTS3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTS3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007114-RA">
        <property type="protein sequence ID" value="MESCA007114-PA"/>
        <property type="gene ID" value="MESCA007114"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1726852_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577701" description="LRRNT domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007114-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17447" checksum="B7F0CCC18B2EBBC0" modified="2013-10-16" version="1">MEGVSKIFLIANIVGLLLFYIPTPSTANCDLRKNEKASKEEETVSLLCSNTTIDHVNNFLLHNYSQYSLNFDEITLKWVNRSHSERLHLNLPKNIVIHNLKWLESTLHDGNLNQLLSVENENNFENLESLDVSGNHIKCLNWANNQFVQGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUE5</accession>
    <name>T1GUE5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Non-catalytic component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02122938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02122939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUE5"/>
    <dbReference type="STRING" id="36166.T1GUE5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007355-RA">
        <property type="protein sequence ID" value="MESCA007355-PA"/>
        <property type="gene ID" value="MESCA007355"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_5_2_1"/>
    <dbReference type="OMA" id="TFIYGYC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000010">
        <property type="entry name" value="Proteasome subunit beta type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007355-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="218" mass="23942" checksum="6C1A4AEE24487A19" modified="2013-10-16" version="1">MVFDNAESWMSAPTALTTIMAVEFNGGVVIGADSRTSTGVYVANRVTDKLTRITDKIYCCRSGSAADTQAIADIVSYSLYYHENQTNEEPLVSEAASEFRNYCYNYRDSLLAGIIVAGWDKKLGGQVYSVPLGGMLIRQPYTIGGSGSSFIYAFAKENFRENMNRDECVEFVKKAIHHAMYFDGSSGGVCRIGVITETGIDRNIFFATESKTSQDQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUK3</accession>
    <name>T1GUK3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007418-RA">
        <property type="protein sequence ID" value="MESCA007418-PA"/>
        <property type="gene ID" value="MESCA007418"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_481713_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007418-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="566" mass="66662" checksum="D38184703E4F5F54" modified="2013-10-16" version="1">MYKRDNPYNFEIPSGPPPKDYQGQARYWCKVLGITPDQLSQMSEQDLENRVQMFQQSYMRNTNKMKSRDDRHPAYQSVMESSGSPRRTRTPRYQEDESRRTPRNENLEKNARKLAAKKAAVAKVQRQLEEEAALEAEERQQRLEQERYALQQQQNQKDNAQRRARRSVPAPVPPRQSARSPDRRQRTESPQYRNSMSPTRATEAATISRGATKAETISGRSTKQEQLFEEQQRQQQMYEEQQRQQQLFEEQQRHQQQINEDNERKRYLKYLEDQFQRKQKELERRRLREEELLLQQRQQALDPEYQTDDIVSNEEGDFTEATEVEDEETYENDYEAEESDDDIKAWCELFGVDENELFDMSETEIHRRLDQCELRKKQAQILKEKTKHLNLKNFKIRSFTKESFRPGEKPLVKIPNDRKSISLKEVRKRGGQSCGCEEEEDSSAMFEILCNAAIELERTYGEDIFGTSDINPCISKNSLTELFISAQGCRGDYFDTSIAFDYDSNTVYFSSKKFTDDQEIITTKEIPLHKNIYECMFDNSLCDRGGDDDGVLQVMTFMANDKGDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUS9</accession>
    <name>T1GUS9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hexosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02137287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUS9"/>
    <dbReference type="STRING" id="36166.T1GUS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007499-RA">
        <property type="protein sequence ID" value="MESCA007499-PA"/>
        <property type="gene ID" value="MESCA007499"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909073_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007499-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15387" checksum="458A8EFB30CE32AE" modified="2013-10-16" version="1">MVKNCPEDTESLLLSSTSNPNEKNIGFRNRRRLHRRCPRMVQTFVIFSVFIVGVFLLIYIPIFSNSNSRMYPLQSWSFNTSRNTADYILPNQETALIEPKNVCDTKKFFLLIMVSSALGNFEARQNIRETGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV66</accession>
    <name>T1GV66_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV66"/>
    <dbReference type="STRING" id="36166.T1GV66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007650-RA">
        <property type="protein sequence ID" value="MESCA007650-PA"/>
        <property type="gene ID" value="MESCA007650"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1196029_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007650-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="232" mass="25031" checksum="E91EF4EC383FD987" modified="2013-10-16" version="1">MDQQMMASKAIELNKDQPDPQPPPLVATSQKSGAIKKKIMTLPHTNNGATAATNNQQAASTTSANQQQQQPQQAQKQQVNSSTIPAVQALDPNKIMPVNITLPAQPGTANTESRVLTIQVPASALQENQLTQILTANLISSIMSLPANLASSVLQQHVNSALQNAKMQSKQLDGAYDTSDEDNSDISDNDIDNDDDDDIDNEGDEEQEGEFLGIFYLVFVIKFENSAILTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV80</accession>
    <name>T1GV80_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02063220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV80"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007667-RA">
        <property type="protein sequence ID" value="MESCA007667-PA"/>
        <property type="gene ID" value="MESCA007667"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1962100_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007667-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="128" mass="14675" checksum="511C46B8458733C7" modified="2013-10-16" version="1">MRQEYRWAKRSIYLITYACYVGNIKSAEYLLLYGANIAAINSCGQSTLNLAIWSGIPEMVKFILSKRSYQQFVKSSLIPPICVAELRNWDSMVTYLKKQHGAESSEQTVHGLNHNHIKMLKNAAYNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV87</accession>
    <name>T1GV87_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02197518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV87"/>
    <dbReference type="STRING" id="36166.T1GV87"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007674-RA">
        <property type="protein sequence ID" value="MESCA007674-PA"/>
        <property type="gene ID" value="MESCA007674"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278943_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007674-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="216" mass="23036" checksum="EFE8021DA61D9CD6" modified="2013-10-16" version="1">MLRDGMLIEARHVKRKQLNQYLDSDFLKQEKRSMENHNEFNNTLLANRKRLSSELAQSKDPQQPANNQANQQTVNTQTKRRRPISESQMEENSNASSVASDGQTTPTTLSSPPSFTNMTSTTAPGGKYSNGNGDEEKSASAATQGAGGGNGTGSGSAGTGTQGTATATSTAEVDEENVDDDITSIIISIIKVEIIVAPWTQNLLKIMLSGALASKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVR1</accession>
    <name>T1GVR1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007877-RA">
        <property type="protein sequence ID" value="MESCA007877-PA"/>
        <property type="gene ID" value="MESCA007877"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123906_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007877-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12499" checksum="5934627A1529B950" modified="2013-10-16" version="1">MANQKSCASKELKEADLKDFSKQCEATPKGEEDYACYIQRLKNNVVVRDCISTAKALSLNINENACDASKVENTKCNFCKGKDCNKGEPRNSGTHLQVVAGLVAFAAIFVTKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVX1</accession>
    <name>T1GVX1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02052004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02052007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVX1"/>
    <dbReference type="STRING" id="36166.T1GVX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007939-RA">
        <property type="protein sequence ID" value="MESCA007939-PA"/>
        <property type="gene ID" value="MESCA007939"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2963419_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007939-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="59" mass="6863" checksum="1DA5BD3AD70BC9E4" modified="2013-10-16" version="1">MYDYPNKASSPTQYYWYRSTLMCSPSVDEPEQINYHMAIALIVSWFLILKGNINLTVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ4</accession>
    <name>T1GXJ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008546-RA">
        <property type="protein sequence ID" value="MESCA008546-PA"/>
        <property type="gene ID" value="MESCA008546"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850173_0_0_1"/>
    <dbReference type="OMA" id="FDLINMN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008546-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15539" checksum="3035DACCFDC3A7D0" modified="2013-10-16" version="1">LYGSENVFTTNDLVFQPLKLSALKEKKALYFPTYMGEDVLSKVKPAEDAETTIEYVDSIISGKKFDLINMNNKVELDLFVLGSVLVSKDGRRIGTVDIIVTPSEVITVENPPQRPTGILWDHISERQLQNSAVLQSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXN1</accession>
    <name>T1GXN1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02053520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008584-RA">
        <property type="protein sequence ID" value="MESCA008584-PA"/>
        <property type="gene ID" value="MESCA008584"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2815354_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008584-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7828" checksum="6CA5AC1C5442A6A0" modified="2013-10-16" version="1">MASLITNKQEVLGRWEDFSRNCVQNSYFENTNQLPALWKEKLGDEELLTAFDRRTLRIIYGSVCEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXW5</accession>
    <name>T1GXW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02373361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02373362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008677-RA">
        <property type="protein sequence ID" value="MESCA008677-PA"/>
        <property type="gene ID" value="MESCA008677"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1724392_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008677-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="152" mass="16617" checksum="F7D05266A5A74AC3" modified="2013-10-16" version="1">MIKLARQAGAKRPLFDGARWEGGHKLNLGRIRKEIGLDDKTTNELCPVPISKDKLLTISGSFSSSAAEKRGMFSKRERSDDNGGESPAKSQSPSTSFHQSEKGIVVGIINENIVGNKVAIDLWKKVEMVLIANMIKLARQAGTKRPLFDGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYU1</accession>
    <name>T1GYU1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02133027">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYU1"/>
    <dbReference type="STRING" id="36166.T1GYU1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009028-RA">
        <property type="protein sequence ID" value="MESCA009028-PA"/>
        <property type="gene ID" value="MESCA009028"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106531_1_0_1"/>
    <dbReference type="OMA" id="CHASTWV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF35">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="29"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="170" mass="18822" checksum="70F45273413D07FB" modified="2013-10-16" version="1">MTETIGKTEIRAWTYEEAISATGVGRFHYYLLATCGFALMAMATEVPGMSIIILAAKCDLNFSLQQQGLLASSGYFGIVLSCQFMGYLADKYGRVKIMRTSMMIALTCSLCSVFSVNTLMLIVLRFLTGIFIAGNQVGFTLIAEYHGNVSRSKHLTYLSTFLVMGSFYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ96</accession>
    <name>T1GZ96_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-nitrophenylphosphatase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02022999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ96"/>
    <dbReference type="STRING" id="36166.T1GZ96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009194-RA">
        <property type="protein sequence ID" value="MESCA009194-PA"/>
        <property type="gene ID" value="MESCA009194"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_0_0_1"/>
    <dbReference type="OMA" id="MIDQDIA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF4">
        <property type="entry name" value="RE04130P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="27"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009194-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="29582" checksum="0DF59DF149F80E96" modified="2013-10-16" version="1">MVVGPIENAGTGFTLLQKAGKSLKFISNNAYRTDADYIKKLSSIGVQNVKPNDLIHPDKTVITYLKSNPKYKHIFAICGPIFREALISNGFTIDDKPDETERFTLQSFTQNIIPTAPVDAVVIDIDLDMSLAKFCRGLLHAKNYPDCDIIVGATDNKIPLSKDVVIPGTQVLMDYIEHYSGKKSIVLGKPGEQLGNHIMELYNISCPERSIFIGDNLSTDIGFANSLGFQTLFVLSGAHTYEDMMNAPVKINPITSQIALGILLSFLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZF6</accession>
    <name>T1GZF6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZF6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009256-RA">
        <property type="protein sequence ID" value="MESCA009256-PA"/>
        <property type="gene ID" value="MESCA009256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3302298_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="40" mass="4156" checksum="94C798EA26617B06" modified="2013-10-16" version="1">MTTITTIQAGAIAKIIVPSTSPLTPNSTDELLPSVDESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZT6</accession>
    <name>T1GZT6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02185930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02185931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZT6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009392-RA">
        <property type="protein sequence ID" value="MESCA009392-PA"/>
        <property type="gene ID" value="MESCA009392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673938_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8594" checksum="6564537817DEF562" modified="2013-10-16" version="1">MEENGSAKKCFFTKPNRYRMKGRLRTRWSDVISRNARLIGVPNWRTTAKNREVGKAAIIQTVYSDCSEDPLAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZW5</accession>
    <name>T1GZW5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009423-RA">
        <property type="protein sequence ID" value="MESCA009423-PA"/>
        <property type="gene ID" value="MESCA009423"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3244921_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009423-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="43" mass="5209" checksum="C18F4A32EA85FED2" modified="2013-10-16" version="1">MPSLDEMENHFMNIFSKDSQQDEEPIQDRRQEQITSNPVEERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZX1</accession>
    <name>T1GZX1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ZAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02085291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZX1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009430-RA">
        <property type="protein sequence ID" value="MESCA009430-PA"/>
        <property type="gene ID" value="MESCA009430"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132433_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009430-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="20355" checksum="87FE387A17945250" modified="2013-10-16" version="1">CANIEVKRNDRLPKYICAQCKEGLESAYRLRKMGEATTKRLQTKLSKSNANKKLNAKASKKPKMNLRRSSRLKDSDKDSKVFEEILDIEEEENIIEYSDVEEDCEELQNCQELEEEDIYEDSEKFEEDEYIPVNVDNSEYDPLNLEITTNSKESNETKSEKPKKPRMKFPKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0I2</accession>
    <name>T1H0I2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphotyrosine protein phosphatase I domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02378968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0I2"/>
    <dbReference type="STRING" id="36166.T1H0I2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009660-RA">
        <property type="protein sequence ID" value="MESCA009660-PA"/>
        <property type="gene ID" value="MESCA009660"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_5_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF7">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="5">
        <location>
            <begin position="6"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009660-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6591" checksum="E0DE576EDBC5EB46" modified="2013-10-16" version="1">MGQKKVLMVCMGNICRSPMAEAIFQDMIDKAGLNEKWAVESAAIGCWDVGNPINYRAVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0R3</accession>
    <name>T1H0R3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SKI-interacting protein SKIP SNW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNW family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02019749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0R3"/>
    <dbReference type="STRING" id="36166.T1H0R3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009749-RA">
        <property type="protein sequence ID" value="MESCA009749-PA"/>
        <property type="gene ID" value="MESCA009749"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006601_3_0_1"/>
    <dbReference type="OMA" id="KARDDCA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="3">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009749-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="28508" checksum="B6014D5D51EF611C" modified="2013-10-16" version="1">VTVKEQKEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQQVHINEKFAKMAEALYIADRKAREHVEERAQLEKKLAQKEKEKKEDALRQMAQRARDERAGLRLPTEDEAGTSKSADFREREELRNERHRERARDRNLSRAAPDKRSKLQREKERDISEQIALGMPTKTNVGGETLFDQRLFNTTKGMDSGYGDDEAYNVYDKPWREGGSMANNLYRPSKSVADNEIYGADYDKIIRARQRVQRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0U5</accession>
    <name>T1H0U5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule-associated protein Jupiter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to all microtubule populations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAP Jupiter family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02004814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0U5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009787-RA">
        <property type="protein sequence ID" value="MESCA009787-PA"/>
        <property type="gene ID" value="MESCA009787"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140572_0_0_1"/>
    <dbReference type="OMA" id="ANQKEEP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF2">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009787-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="122" mass="13210" checksum="D5FDB568C15C5B43" modified="2013-10-16" version="1">MTSTELFQGMKPTARPSSRVLKPPGGGHTSIFGDVEAEPNKPRPKYNQQNSSNLNQCMNTVDPNESVEKTRQELAQKATDQKNSEASEKKSEPVQVKEDNGNGMDSGKGRVPPGGFSSGGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H113</accession>
    <name>T1H113_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02375498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02375499">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H113"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009864-RA">
        <property type="protein sequence ID" value="MESCA009864-PA"/>
        <property type="gene ID" value="MESCA009864"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874308_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009864-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7745" checksum="DF6353179BC3641D" modified="2013-10-16" version="1">FFNYIFKNLLNLKSIELFKNIVHLLERSPVTRCYLAVAQLSFLPKKKHIGAYYTKHHFTHNYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H135</accession>
    <name>T1H135_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H135"/>
    <dbReference type="STRING" id="36166.T1H135"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009887-RA">
        <property type="protein sequence ID" value="MESCA009887-PA"/>
        <property type="gene ID" value="MESCA009887"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2624798_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009887-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="8842" checksum="95FA1B6EA5F0F317" modified="2013-10-16" version="1">MLLDGMKEKIAADKRKITPVTSSNEASSEDSNDSNSHDSNKNFQNKETKDEMTSQSVDSHEIDNIMGSYFYTFFIILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1E9</accession>
    <name>T1H1E9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037807">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA010008-RA">
        <property type="protein sequence ID" value="MESCA010008-PA"/>
        <property type="gene ID" value="MESCA010008"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725118_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588656" description="Peptidase S1 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010008-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="7989" checksum="8E88E14C36DCF822" modified="2013-10-16" version="1">MRSSSASRLLSRGFLLILALCEVGSLDYNYKSQNYRQGAVVSNGEMCAGIGRKIFILYELLSKCLFCSKTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1Q8</accession>
    <name>T1H1Q8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
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    </organism>
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        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="MESCA010125"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045277_11_3_1"/>
    <dbReference type="OMA" id="FLEYKHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:chromatin remodeling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HFD_CHRAC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="3">
        <location>
            <begin position="9"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010125-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12946" checksum="45842827DB2BF97C" modified="2013-10-16" version="1">MQNFTNEFQLPPTRVRTIMKSTEDAANISQEASLMVTKATEMFISELAVKTFKNGKKGNLLKYDDLAEFVSEENKLEFLLQIIPKKMSIKEYLEMAAKDEHDSEESSSESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1H237</accession>
    <name>T1H237_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02381989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H237"/>
    <dbReference type="STRING" id="36166.T1H237"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010268-RA">
        <property type="protein sequence ID" value="MESCA010268-PA"/>
        <property type="gene ID" value="MESCA010268"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085098_0_2_1"/>
    <dbReference type="OMA" id="TISEFEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000045">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1220.10">
        <property type="entry name" value="EGF-type module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF5">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010268-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="152" mass="17009" checksum="690DA9818CB4FCA8" modified="2013-10-16" version="1">CEPHQFQCGNGIQCNGIRDCFDNSDEKDCSVPDTCYPNQFFCAYQQKCISDTLRCNGRNDCGDNADELDCQTSVKSCNSNEFLCANEGRCIPDYMICDGRNDCGDYSDERSCECEDGLLCFDQKCISKSKQCDNVDDCAYGEDEINCELTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2C7</accession>
    <name>T1H2C7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2C7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010367-RA">
        <property type="protein sequence ID" value="MESCA010367-PA"/>
        <property type="gene ID" value="MESCA010367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402145_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="11105" checksum="A48C4B30E90EDDCA" modified="2013-10-16" version="1">MIGVNICTYHGISTSETPCMMCATKHMIFEHFAICDVVQALLSYRFLVDVLLYHFRFLVYNNLYHIKSKDGRHFREQNREFLNDLSSLLKYRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2J8</accession>
    <name>T1H2J8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02386977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2J8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010446-RA">
        <property type="protein sequence ID" value="MESCA010446-PA"/>
        <property type="gene ID" value="MESCA010446"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2888308_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010446-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7141" checksum="2FD131C732ED2E07" modified="2013-10-16" version="1">MLKKLHDSASPCQKPLTFWNVLVDIPAAFTQLMPTLNTGSQFARRMFYTLSYGSLNGSHHKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2V3</accession>
    <name>T1H2V3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2V3"/>
    <dbReference type="STRING" id="36166.T1H2V3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010559-RA">
        <property type="protein sequence ID" value="MESCA010559-PA"/>
        <property type="gene ID" value="MESCA010559"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309188_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577836" description="Peptidase S1 domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="48"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11479" checksum="D37C6DAB4317D4C3" modified="2013-10-16" version="1">MMLILKLFFLTFFISFSYCNNLYHYKEEQQLPKPPDCGTVQHLTKRLYGGSPINITNIPWMVALEFRDDGNSTTIECGGSLINNRYVVTAAHCIEDEDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Y3</accession>
    <name>T1H2Y3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Y3"/>
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        <property type="protein sequence ID" value="MESCA010589-PA"/>
        <property type="gene ID" value="MESCA010589"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1688742_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010589-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="17771" checksum="5B4C69082472146D" modified="2013-10-16" version="1">MKFQEFVPIDKFVYGNGSRETRIKFYFQGISHFGLGLLSQPAEPNTLDPILNFQISCEPNTQNERSYIYLNTAETKHCASKGFRNPMNNIYFTEIEIVINKEFGLVAAFLPESGSTPFLSCQGPEISAMISKVKYIGLTGRNENVVRYIYDCPEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H318</accession>
    <name>T1H318_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H318"/>
    <dbReference type="STRING" id="36166.T1H318"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010629-RA">
        <property type="protein sequence ID" value="MESCA010629-PA"/>
        <property type="gene ID" value="MESCA010629"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852276_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010629-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7437" checksum="1CCFB957F890E606" modified="2013-10-16" version="1">MMHIKERKEMEDSFKKAQKPWAKLLAKVEKAKGDYHAACKTEKTAINQERNATADSSLSPDQILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3E7</accession>
    <name>T1H3E7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3E7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010772-RA">
        <property type="protein sequence ID" value="MESCA010772-PA"/>
        <property type="gene ID" value="MESCA010772"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471617_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010772-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9799" checksum="6822E5D7BB4A2864" modified="2013-10-16" version="1">MLSCVKAAVESPTVSRLVENHLENSRLNMNSSWYSQNSMMLIGQKDTALKTSREIIEIKNTGYSISKTKLGWTISGPIFATPDDSNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3T9</accession>
    <name>T1H3T9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02384501">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3T9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010927-RA">
        <property type="protein sequence ID" value="MESCA010927-PA"/>
        <property type="gene ID" value="MESCA010927"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2892479_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010927-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="63" mass="7070" checksum="F9508800C6A9A9F3" modified="2013-10-16" version="1">MQILSQGNKIAGWALSVIYSVRVNYVICLFLLQDNASSCQTPLFVRKFSVVLPADLTLLHVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3V0</accession>
    <name>T1H3V0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02379549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3V0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010943-RA">
        <property type="protein sequence ID" value="MESCA010943-PA"/>
        <property type="gene ID" value="MESCA010943"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3422607_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010943-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="24" mass="2750" checksum="681A46E3AB4700D7" modified="2013-10-16" version="1">MVCSFDTLYNAVQPQYTLEVRTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5C4</accession>
    <name>T1H5C4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(+) ADP-ribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02054939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02054941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5C4"/>
    <dbReference type="STRING" id="36166.T1H5C4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011501-RA">
        <property type="protein sequence ID" value="MESCA011501-PA"/>
        <property type="gene ID" value="MESCA011501"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145017_0_0_1"/>
    <dbReference type="OMA" id="WESSKAT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08001">
        <property type="entry name" value="WGR_PARP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="WGR" evidence="7">
        <location>
            <begin position="12"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011501-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27828" checksum="63EB4B3EADC16A98" modified="2025-10-08" version="2">MTVDPGSGLESICKVFVSGNEKYSVVLGVTDLNTNRNAFYKIQLLVSEDERRFWIYRAWGRTGTSIGGDKTEGFANLERAQANFKATYFEKTGNEWENRHDFVKKPGLFYPIDISYAGDAKVDWESSKATSNLPKATQELIKLIFDIDSMKKTMLEFDLDMEKMPLGKLSKDQINKAFDVLNEISHYIKYGATEMDFIDASNRFYTLIPHNFGMRSPPILNELYQLNDLNSMLNTLLQIECAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5S1</accession>
    <name>T1H5S1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H5S1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012054-RA">
        <property type="protein sequence ID" value="MESCA012054-PA"/>
        <property type="gene ID" value="MESCA012054"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000138">
        <property type="entry name" value="Phosphorylase b kinase gamma catalytic chain, liver/testis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012054-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="293" mass="33614" checksum="DC410846EC672348" modified="2013-10-16" version="1">MAKEEDDEDLLPDKDAAKGFYAKYEPKEILGRGVSSSCKKTEKKFAAIIIDLGAGTEPSDTNLQQMVEATRQEISILRQVLGHANIIDLQDVFESDAFFFLVFELCPKGELFDHLTSVVALSEKTRNIMRQIFEGXXXXXXXXXXXXXXXXXWACGVIMLTLLVGCHPFWHRKQMVMLRNIMXEDPKDLIRKCLVVDPIKRITVHEALKHPFFNQMVRAFIRIKRLRYTAEPLRVEETVQDSYTSKVLRKVIDGCTFRVYGHWVKKGEGQNRVALFENTPKTELNALYLNNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H694</accession>
    <name>T1H694_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pheromone biosynthesis.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H694"/>
    <dbReference type="STRING" id="36166.T1H694"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012227-RA">
        <property type="protein sequence ID" value="MESCA012227-PA"/>
        <property type="gene ID" value="MESCA012227"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2475310_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042811">
        <property type="term" value="P:pheromone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012227-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="9738" checksum="72620EE86A221A67" modified="2013-10-16" version="1">IIFITTAGQANDLQTSSKSVDCFNMDTYKRIAANKTSYYTFYLPVALAMNMAIGNAISSKSKMISWTVLEIQMSLAKELIFKKTNALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6I5</accession>
    <name>T1H6I5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6I5"/>
    <dbReference type="STRING" id="36166.T1H6I5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012318-RA">
        <property type="protein sequence ID" value="MESCA012318-PA"/>
        <property type="gene ID" value="MESCA012318"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929426_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000022">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="19"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012318-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="7147" checksum="2A7965A55DB0FC4E" modified="2013-10-16" version="1">IQRNKEKLTLTEKESLLTQGFTSWTKRDFNQFIKANEKYGRDDIESIAKDVEGKTPEEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Q5</accession>
    <name>T1H6Q5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6Q5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012388-RA">
        <property type="protein sequence ID" value="MESCA012388-PA"/>
        <property type="gene ID" value="MESCA012388"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026811_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051759">
        <property type="entry name" value="LIM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218">
        <property type="entry name" value="LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218:SF4">
        <property type="entry name" value="LIM AND SH3 DOMAIN PROTEIN LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="148"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012388-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="194" mass="21893" checksum="66B65CA64161A9E7" modified="2013-10-16" version="1">MNKTCARCTKVVYPIEELKCLDKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRLFRAIYDYEAQDTDEVSFREGDLIEVDSIDSGWMTGKVDRTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6U1</accession>
    <name>T1H6U1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dynein assembly factor 3, axonemal homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNAAF3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H6U1"/>
    <dbReference type="STRING" id="36166.T1H6U1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012423-RA">
        <property type="protein sequence ID" value="MESCA012423-PA"/>
        <property type="gene ID" value="MESCA012423"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024420_2_1_1"/>
    <dbReference type="OMA" id="DWCETGI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039304">
        <property type="entry name" value="DNAAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028235">
        <property type="entry name" value="DNAAF3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027974">
        <property type="entry name" value="DUF4470"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22118">
        <property type="entry name" value="DYNEIN ASSEMBLY FACTOR 3, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22118:SF14">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14737">
        <property type="entry name" value="DUF4470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14740">
        <property type="entry name" value="DUF4471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4470" evidence="5">
        <location>
            <begin position="2"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein assembly factor 3 C-terminal" evidence="6">
        <location>
            <begin position="133"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012423-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="46709" checksum="C3A7FE6D8A420E82" modified="2013-10-16" version="1">MFWGFSEALNFYEEFKDISSKNDPEELNILLFGNTDSRHIIKTLAKSYKHNVKVNFYVLDGCIEIVARNMLLLSIALDDPLQLTVKSKTHLFMDIFGNSLLRPISYNYMASRAGFLRKLITDSDFLPEKAPIFNIDGLKYRERDGLEDAFNFWLPSEKHVFNISKSGKIIGIGARFDYKKGGFDWDLQMCLKEYTYWRDTGVAFTFPEYEQCNPNKTLVLDYLSKDDYSHRGYVGDMVGPFAVLDSKLRTNKVGLHGDNDYRATDLTEANLFEMFYELSKELPLEDIDCHKYGNIILGMSELLSSKGEENIELKSYNEPFLKFPNIKINFLPLEDAFEIPKGEIYKSKFDFIFVASNYLDFVKAPILETLKDEGSIYFETKTFPMNGDFEFLETYSDFSKENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Z3</accession>
    <name>T1H6Z3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6Z3"/>
    <dbReference type="STRING" id="36166.T1H6Z3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012475-RA">
        <property type="protein sequence ID" value="MESCA012475-PA"/>
        <property type="gene ID" value="MESCA012475"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3093471_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012475-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="52" mass="5768" checksum="8AC22AD539AA5384" modified="2013-10-16" version="1">KKINFNFINMLTDMTPTAGHQLYGSQISAMGMNITNIASHIQKPSVNTVSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H781</accession>
    <name>T1H781_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glycerol + ATP = sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="T1H781"/>
    <dbReference type="STRING" id="36166.T1H781"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012619-RA">
        <property type="protein sequence ID" value="MESCA012619-PA"/>
        <property type="gene ID" value="MESCA012619"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_7_0_1"/>
    <dbReference type="OMA" id="INTWILW"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000177">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF82">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="13">
        <location>
            <begin position="266"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012619-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="328" mass="35727" checksum="EF1D5E1E896D1B22" modified="2013-10-16" version="1">YGPLVGAIDEGTSSARFMIFKAASSEIVCYDQLEVISIPRKDGEQDPLVILDIVEKCMEGAIKKLEELGIKDIVAIGITNQRESTIVWDKTTGKPLHNAIIWLDMRTSSTVDHLVDTIPNNSRNKNYLKPLCGLPISTYFSALKIRWLKDNVNSVKQAMKLKTAFGTVDTWLLWNLTGGLSGGVHITDVTNASRTMLMNIETLQWDSHLLKFFDLPKHILPKICSSAEIYGNVYKGSLKGVPISGCLGDQQAALVGQCCLKRGQAKATYGTGCFLLYNTGSTIVNSTHGLLTTVGYQLGKKGTPVYALEGSVAIAGAAMGWLKDNIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7B4</accession>
    <name>T1H7B4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H7B4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012652-RA">
        <property type="protein sequence ID" value="MESCA012652-PA"/>
        <property type="gene ID" value="MESCA012652"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020445_3_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="3">
        <location>
            <begin position="23"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012652-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="431" mass="48352" checksum="B8BFAC8C7C20714B" modified="2013-10-16" version="1">MSERADNAAEAFDDQVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTKQDGKDYSVHRLILGTHTSDEQNHLLIASVQLPNEDAQFDASHYDNEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNACVIATKTPSSDVLVFDYTKHPSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWDLSKIGEEQSTEDAEDGPPELLFIHGGHTAKISDFSWNPNEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7C9</accession>
    <name>T1H7C9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H7C9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012666-RA">
        <property type="protein sequence ID" value="MESCA012666-PA"/>
        <property type="gene ID" value="MESCA012666"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2851981_0_0_1"/>
    <dbReference type="OMA" id="NACAAEF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034595">
        <property type="entry name" value="NDUFAF8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34561">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34561:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012666-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7408" checksum="2288254820FA74A2" modified="2013-10-16" version="1">MESVRKAKKRLNNYPILLSKCAKSASVYAACVTRDFNVQHKACDGEFQEFRKCLQKAALDLKTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1G9Y5</accession>
    <name>T1G9Y5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="5">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CAQQ02138975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1G9Y5"/>
    <dbReference type="STRING" id="36166.T1G9Y5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000027-RA">
        <property type="protein sequence ID" value="MESCA000027-PA"/>
        <property type="gene ID" value="MESCA000027"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_594879_0_0_1"/>
    <dbReference type="OMA" id="FIYSKEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF2">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="6">
        <location>
            <begin position="355"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000027-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="460" mass="51917" checksum="69947FFE4C7CB077" modified="2013-10-16" version="1">MDALWKLRNRGSDLMGTVLNVNSGDWVRRDSGVGAGIDSYYEYLFKSYILLGNEKYLNRFNRHYDAIMKYISEGPMLLDVLMHRPHAKSRNFMDSLLAFWPGLQVLSGDLKPAVQTHEMLYQVMQMHNFIPEAFTVDFQVHWSQHPLRPEFIESTYFLYKATKDHHYLQVGKKALKALQKYAKVPCGYAAVNDVRTGKHEDHVQETLPSSFYEKSCPSSNSLLPEKIRQPLKNFITGSCPRTSTNKRLRALDFQANSAVHLRAIYDMGITIRDLGDGKAKSPEDGQIGLLFMQEMLELTKFQNQIGQLQVIILKLNDGSSTVLTAGPSHFGKELKDGQFIEGEVEISKPLKCCSNLENAESISQKIVLSERGDCTFVEKARRAEKSGAIGLIICDNNAGTSSDTESMFAMSGDGNNDVTIPVVFIYSKECNILKSNLKPNVKIMQMVDYKKELENNKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAC2</accession>
    <name>T1GAC2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Insulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of a B chain and an A chain linked by two disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the insulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAC2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000176-RA">
        <property type="protein sequence ID" value="MESCA000176-PA"/>
        <property type="gene ID" value="MESCA000176"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125164_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.100.10">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022352">
        <property type="entry name" value="Ins/IGF/rlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016179">
        <property type="entry name" value="Insulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022353">
        <property type="entry name" value="Insulin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13647:SF4">
        <property type="entry name" value="INSULIN-LIKE PEPTIDE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13647">
        <property type="entry name" value="INSULIN-LIKE PEPTIDE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00049">
        <property type="entry name" value="Insulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00276">
        <property type="entry name" value="INSULINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00078">
        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00262">
        <property type="entry name" value="INSULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577206" description="Insulin-like domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Insulin-like" evidence="8">
        <location>
            <begin position="33"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000176-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="130" mass="14598" checksum="75B8EEA71C6EF4FF" modified="2013-10-16" version="1">MKFFTTVCIVLACLALLVSQSEGQIKFGEGGKVRLCGRLLTSTLQNLCVNGLYTNLKKKSVPETTYDDDVSVDGNFDSEGDMAPFYPSFDDKIFMNKGSMLSKWRRRRNGIVNECCHKQCSINTLLTYCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAT0</accession>
    <name>T1GAT0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAT0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000341-RA">
        <property type="protein sequence ID" value="MESCA000341-PA"/>
        <property type="gene ID" value="MESCA000341"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191714_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9570" checksum="D05D806ADEBE563E" modified="2013-10-16" version="1">MVTSLCDESVKNSSANCDKSRMTPQKESLDFNCNEDIPSLTTEEVRNIIQRFKYNKSAGSDGIPAEVLKSAGINFIDSFHELLVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAT1</accession>
    <name>T1GAT1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02013289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000342-RA">
        <property type="protein sequence ID" value="MESCA000342-PA"/>
        <property type="gene ID" value="MESCA000342"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545203_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000342-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9866" checksum="8781D32A25FD5732" modified="2013-10-16" version="1">MYSGLTFQFSSRIVPCSSLKEDELDRMMIMRTKDTKTALKSKEMVEKKRIWQKIMLKTAGRSARAHWKKRNFYRKKRSFCPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBK0</accession>
    <name>T1GBK0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02171025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBK0"/>
    <dbReference type="STRING" id="36166.T1GBK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000634-RA">
        <property type="protein sequence ID" value="MESCA000634-PA"/>
        <property type="gene ID" value="MESCA000634"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_7_2_1"/>
    <dbReference type="OMA" id="FRMATIQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577243" description="SCP domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="57"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000634-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="20181" checksum="659C2100EBEDAB6E" modified="2013-10-16" version="1">MFLKYLIVLAILFIAASATDYCNPDLCRQGKAHIGCNNNGKLSSSCPANSQFFNMQVLKNQIVRAHNEKRNRVAGGFIPKHSPAFRMATIQWDDELAYLAGLNVKSCKYGHDQCRNTDTFEYSGQNLAKTWWKGMNQNISMLIQDNIEGWFDEYKKSDMSKINKSLTKRNQLLDTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBR2</accession>
    <name>T1GBR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02198381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBR2"/>
    <dbReference type="STRING" id="36166.T1GBR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000699-RA">
        <property type="protein sequence ID" value="MESCA000699-PA"/>
        <property type="gene ID" value="MESCA000699"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_6_1_1"/>
    <dbReference type="OMA" id="TLCNVQI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000047">
        <property type="entry name" value="tyrosine--tRNA ligase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="9">
        <location>
            <begin position="134"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000699-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="290" mass="31735" checksum="07D2AF3C97C2E495" modified="2013-10-16" version="1">MSANTADFMRKNQEAAALLKAREFEVQQIVNELVTRRKAELESENDSLRQQIEAAKKNLIALETLNGRKQVSIPVRNFSTDTTQAPQPVKEEPKESQPAAVSVATESKPQKEKKEKPKKEKAAAPPAAEEPPIDVGRLDMRVGKIVEVSRHPDADSLYLEKIDCGEPNPRTVISGLVKFVPIEQMQNRMVVVLCNLKPAKMRGILSEAMVMCASTPEKVEVLSPPAGAVPGDLVHCEGYERHPDAQLNPKKKIFETCAPDLKTNDSLIATYKGAVLNVPGKGNVVSQSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBT0</accession>
    <name>T1GBT0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBT0"/>
    <dbReference type="STRING" id="36166.T1GBT0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000718-RA">
        <property type="protein sequence ID" value="MESCA000718-PA"/>
        <property type="gene ID" value="MESCA000718"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041209_0_0_1"/>
    <dbReference type="OMA" id="HSFGTER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34717">
        <property type="entry name" value="EG:BACR7A4.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34717:SF1">
        <property type="entry name" value="EG:BACR7A4.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000718-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="422" mass="48933" checksum="E4EE959BFCCBA4F6" modified="2013-10-16" version="1">MVAVGYVLFGIVGGIITLLLLVLGILRTNPERIFNVYPKKGLLYHLKYYLVLQLVKYFRERICQKYIRRNSIDYIGGIYTMDAPQELSQNPKSYDKVSFIGSTQNGHRLVVSVERRKKGIHYTTLYLYTPNRGVFKLPNLPDTLCVPETEVELETNEYNANGIKLEVLDPMRKWKIEYNGQLMNNGKLYNVAIKAEFNSKNSSYFNHNKDLSSFVLADSLAREPWNDDFFHILKNIMTMQAFRDHSFGTDGFLETMHRYAYFVIFLNNGSYVVFGNLSRPSFFLSSLKVGYIVTKDGRYLPLKDSNFELYSYGETGRPPKHSNFVISTEDNKIFTSIVCESYEKRYLGANWEGKIYNQFISCGVGSQSGHGVAEYFYRNTTGRPENFQLKDPNWFRSVQNFERSISECHHTEDEEDDDDFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC13</accession>
    <name>T1GC13_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02105617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC13"/>
    <dbReference type="STRING" id="36166.T1GC13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000808-RA">
        <property type="protein sequence ID" value="MESCA000808-PA"/>
        <property type="gene ID" value="MESCA000808"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040364_2_0_1"/>
    <dbReference type="OMA" id="CMVKKPK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14280">
        <property type="entry name" value="UBA1_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000254">
        <property type="entry name" value="UV excision repair protein Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="137"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000808-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19041" checksum="8CD662E43D88F5A3" modified="2013-10-16" version="1">MLITFKTLQQISFTIEVDPKISVAELKTEIFKKNGPDFPAENLNLIYAGVILDDARNVESYNIDEKKFIVVMVKKLNVASSSSASSTSAAASETPKQPPVEKKEETQQPEQKKEEVAVAAVSAAAAAPEPTASISGDLDETVKSIMEMGYDRQMVEQALRASFNNPERAVEYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC20</accession>
    <name>T1GC20_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02395079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000815-RA">
        <property type="protein sequence ID" value="MESCA000815-PA"/>
        <property type="gene ID" value="MESCA000815"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3360240_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000815-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="36" mass="4192" checksum="AA9FF0A3C7AEA6CF" modified="2013-10-16" version="1">MKIVDKNSAIIILGEFQFSKKKKIFYKIFGYSASTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCF3</accession>
    <name>T1GCF3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02150607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GCF3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000964-RA">
        <property type="protein sequence ID" value="MESCA000964-PA"/>
        <property type="gene ID" value="MESCA000964"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000847_0_0_1"/>
    <dbReference type="OMA" id="FQIAQEE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045169">
        <property type="term" value="C:fusome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045170">
        <property type="term" value="C:spectrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042062">
        <property type="term" value="P:long-term strengthening of neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11808">
        <property type="entry name" value="SH3_Alpha_Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000154">
        <property type="entry name" value="Alpha spectrin, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000007">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000037">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000020">
        <property type="entry name" value="spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000032">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000006">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000020">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000035">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000043">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035825">
        <property type="entry name" value="Alpha_Spectrin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0117">Actin capping</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="829"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="2043"/>
            <end position="2078"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="2086"/>
            <end position="2121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="680"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1042"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000964-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2193" mass="253024" checksum="42E72D5BD0DD23BD" modified="2013-10-16" version="1">SKDPQTVEDIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELENWINEKLQAASEESYRDPINLQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMINQNHFASGEIQRRLDELHRLWELLLNRLAEKGQKLQQALVLVQFLRHCEEVMFWIKDKEAFVTANEFGDDLEHVEVLQRKFDEFQKDMASQEYRVTEVNQLAEKLIQDGHPEREVITKKKDELNEAWQRLKQLAIVRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAESQRLCAIHGDHSDQIRKKQAEIADYWQTDELAKDVAGAEALLERHQEHKGEIDAREDSFKQTTESGRNLLEREHYAAAEIQEKLAALENDKSSLLSLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDIFATKLIDGSHYAADDVAQRRQMLLARRSALQEKSAARRQLLEDSNRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITSLGTELIEKKHYASDQINQRTQEIVVLWETLVQASDKKGCKLQEACQQQQFNRTIEDIECVQQLFRDLEDEAAWIREKEPIAGSTNRGRDLIGVQNLIKKHQAVLAEINNHEGRLINVLNSGEHMLKDDSQIARDEIRQRINAVQEQWNNLKDKSAQRKQDLEDSLQAHQYFADANEAESWMREKEPIVSGQDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQDQAQSCRQQETPAVDITGKECVVALYDYVEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYVKKIEAGLSANKLNETVKAYVLVREAADLASWIKDKENHAQIAETVCEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLGQTEAALKIQSQMQDLNENWTKLQQLTVEKANQLGSVHEVQRFHRDIDETKDWIAEKENALNNDDLGKDLRSVQTLQRKHEGVERDLAALRDKVGQLEDTANRLANTHPDSAEITLSKQQEINEMGKSHRAEIDFLYGANILPEVHRAMPRNFTEFFSFFEHRTEIDARAGTFAAFEQFGNELLQANHYASPVIKEKIDDLAHAREDLEKAWAERRLQLEQNLDLQLYMRDCEQAENWMSAREAFLNAEEVDSKGDNVEALIKKHEDFDKAINGHEEKIAALQVLADQFITQNHYASDDIDDKRKQVLDRWRHLKEGLIVKRSHLGEKLSLQEFAREADEIQNWIMEKSQLATEESYRDPANIQSKHQKHQAFEAELAANADRIQNVLQMGSNLIDKKQCSGSEDAVQKRLTQIADQWEYLTQKTTEKSLKLKEANKQRTYIAAVKDLDFWLETSTCRTDIQAHEDRIKDMNSQAESLVGSGQFDSAGIQEKCQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKKLLVSSDDYGRDLTGVQNLKKKHKRLEAELASHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWDELKNVSATRGQKLDESLIYQQFLAQVEEEEAWITEKQQLLSVGDYGDSMAAVQGLLKKHDAFETDFAAHGERCTAICNQGTELVESKNHHGDSISQRCQQLRTKLDNLNGLAGRRKNALLDNSAYLQFMWKADVVESWIADKENYVKSDEYGRDLSTVQTFLTKQETFDAGLNAFEQEGIHNITALKDQLINANHAQSPAILKRHGDVLDRWNKLREASEARKQRLLAMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALREAHAQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWFTMEALEETWRNLQKIIEERDGELIKEAERQEGNDKLRKEFAKHANLFHQWLTETSLSINIDDGRIRFFGTTTGSSSYQGYRGRARRVDLKKIEELGALLEEHLILDNRYTEHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEDGQPDPEFDAILQLVDPNHDGHVSLQEYIAFMISKETENVQSFEEIENAFRAITASDRPYVTKEELYCNLTKDMADYCVQRMKPYTDGKSGHEVKDALDYIEFTRSLFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCI7</accession>
    <name>T1GCI7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02012843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCI7"/>
    <dbReference type="STRING" id="36166.T1GCI7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001001-RA">
        <property type="protein sequence ID" value="MESCA001001-PA"/>
        <property type="gene ID" value="MESCA001001"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760712_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001001-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8762" checksum="00AB337D1597D10A" modified="2013-10-16" version="1">MALVPSYKTDWDLWDYRRRLWRDYDWELDYPYWSRLYRHKSLPDLSNVVVNKEGFEATVDVHLFKSYEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCL1</accession>
    <name>T1GCL1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysozyme</fullName>
            <ecNumber evidence="2">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02180735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCL1"/>
    <dbReference type="STRING" id="36166.T1GCL1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001026-RA">
        <property type="protein sequence ID" value="MESCA001026-PA"/>
        <property type="gene ID" value="MESCA001026"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111620_2_1_1"/>
    <dbReference type="OMA" id="VYERCEF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16899">
        <property type="entry name" value="LYZ_C_invert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407:SF63">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00263">
        <property type="entry name" value="LYZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="3" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576822" description="lysozyme" evidence="6">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001026-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="129" mass="14767" checksum="8C5750757F9D6290" modified="2013-10-16" version="1">MKTSFVLLLISLGVLSVQGKIYSKCELARTLSQNGIPRSELPDWDYGIFQINDHWWCKPSNGRFSYNECGLQCERDLLSDDITKAITCAKKIKGRQGFKAWYGWQKKCQGKSLPSFEVGIFNEKEISHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCP6</accession>
    <name>T1GCP6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02080561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02080562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCP6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001066-RA">
        <property type="protein sequence ID" value="MESCA001066-PA"/>
        <property type="gene ID" value="MESCA001066"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2064128_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001066-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="119" mass="13956" checksum="1CD4C2BAE6001BCD" modified="2013-10-16" version="1">MASFLNESNQPHLELMVIFGSIMVYFGVEIMNFVLSPFICRPSSKSYLFGLGITMTMFDFIRVLISRIDVGVEKLRYDCCNQSFTITAAEIRQSQIYWQSYKQSQMISQEDRHFGWRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD75</accession>
    <name>T1GD75_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02077261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02077263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD75"/>
    <dbReference type="STRING" id="36166.T1GD75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001256-RA">
        <property type="protein sequence ID" value="MESCA001256-PA"/>
        <property type="gene ID" value="MESCA001256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1772755_0_0_1"/>
    <dbReference type="OMA" id="MSRNTHT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF36">
        <property type="entry name" value="PROTEIN SINGLE-MINDED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="147" mass="16684" checksum="3B22870D23E9F9D8" modified="2013-10-16" version="1">MMEKLDNLHKMEDYPLYILCKGQVTTKYYRFLTKGGGWVWIQSYATMVNNTRSSRPHCIVSINFVISEKEGKEHILNEIQNEIKPVNHSSPATPLNVNPVCRAASRNTRSNNYSNTSAGSVISESVEYLASTNTQISQECTYNHFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDD0</accession>
    <name>T1GDD0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDD0"/>
    <dbReference type="STRING" id="36166.T1GDD0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001314-RA">
        <property type="protein sequence ID" value="MESCA001314-PA"/>
        <property type="gene ID" value="MESCA001314"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_812075_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF0">
        <property type="entry name" value="PROTEIN DEAD RINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="6">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001314-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="342" mass="39297" checksum="D1B1E51205D14CCB" modified="2013-10-16" version="1">MSKQVLDLYELYRLVCEKGGVLEVINKKLWQDIIKRLRLPSSITSAAFTLRTQYIKYLYPYECEKRNFSDPSELKAVVEGNKRERDNRNSFEDTRTHISNNMGIYSNSSPLNNDSVNSLLGGFYPTPVASNDNLNPLLGGMFNGFDQSPLALLTRRNLMLQYMQMMETQQNPETTCEETKIEEDPSTTPRIKREHPIRSNDFYVNDSVSSSYLSRRTIPNPVYVPHNNHHSVVDGNISSNSDEDEFNTSGTVGENSYTSDFSRRLLNLRAQTLRNSNNFTNQQREIPQVKTQNFTRQSSYSLDRYLTTANLPSLTISKRITSYFKGLSSFEFFSTKTTHLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDE9</accession>
    <name>T1GDE9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4794 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02154884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDE9"/>
    <dbReference type="STRING" id="36166.T1GDE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001334-RA">
        <property type="protein sequence ID" value="MESCA001334-PA"/>
        <property type="gene ID" value="MESCA001334"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1181035_0_0_1"/>
    <dbReference type="OMA" id="APQQRFM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588206" description="DUF4794 domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001334-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="27386" checksum="E59AB065E0F5859F" modified="2013-10-16" version="1">MKVAIVILGLLACSMAVPVKRQAYRPVMYVPHAQRLMYVQYVQPAQPNARSNGVATFVAGDSVATGSYVKEAPVEETPVEDEAAPALKGNTAQAVAEAYPAPLEDDEEEEGQVADDVAADEAPTAPVADEEEEAQNDAPQDEEAPVVVPVKRYGPKKVYVELDAPADDAEYDYAPPARIPVYAAPQARPTNRPTTKKPQQQGQLPAGTFFPINFGGTNGGAIAIANSFSTGEGGSATSHAVAYGSQDALRARKVAARRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDK3</accession>
    <name>T1GDK3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02123386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDK3"/>
    <dbReference type="STRING" id="36166.T1GDK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001392-RA">
        <property type="protein sequence ID" value="MESCA001392-PA"/>
        <property type="gene ID" value="MESCA001392"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037165_0_0_1"/>
    <dbReference type="OMA" id="KWEVEYR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000967">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF100">
        <property type="entry name" value="FASCICLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="17"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="113"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="193"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001392-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="36068" checksum="D2AF8B9310545625" modified="2013-10-16" version="1">MIISLFPGSSYNTVPSAPQDIKAIPASSTKIIISWLPPSSPNGELTGYTFYMTVLEGRHEGTHKRILGPFVEMHETQRTQDNATYQFWLTASTKVGEGEKTQVVIVPPNNKVPARIASFSQVIVTPWKENLMLPCRKVGNPPPVTIWRQDGHSMDTNSRRTLAKNGTLFIRDSQSSDAGNYTCSVNNTWGKDEIVYTVIIKVPPDSPNLTVVNTYTDSYVINYKRDNGDWEELQIDSHTNSHLLSNLWCGTRYQLYITAYNKIGTGLPCDIVNSFTKGNPPVQPKHSQMITNNSTSVTCWLDSWGDGGCGISYFMIECRLYGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDM0</accession>
    <name>T1GDM0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02196154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDM0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001409-RA">
        <property type="protein sequence ID" value="MESCA001409-PA"/>
        <property type="gene ID" value="MESCA001409"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_964079_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001409-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="289" mass="34155" checksum="41673ED8D45B6CA1" modified="2013-10-16" version="1">MSYEETSFFQETLSQITNNISNNRNMMQSMCEEFESKESVFMSSFEKSEKENRKLKNIISELNKEKERFEDQIINLKETADLYHTYYLKIYNTLPIQEAKLLNIENAEQSQNIAKEALENKETLEKELFLLKELLKESDNCKNSFEKQLSELLEKDSNNEVEIKNLKSKLDNFTENYDLLLKKYNELETTNDNCLLEKHILQEANKDITNEKHLITEEFNKKISDFENKISNMEINLKEARSSLSRKDQEIKNLNNKLVQSINDLNDVKTDYNTIGNHTDNLKLEMEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDM4</accession>
    <name>T1GDM4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02152214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDM4"/>
    <dbReference type="STRING" id="36166.T1GDM4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001413-RA">
        <property type="protein sequence ID" value="MESCA001413-PA"/>
        <property type="gene ID" value="MESCA001413"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461112_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF3">
        <property type="entry name" value="FI21118P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001413-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10309" checksum="4393DD660F06FD2C" modified="2013-10-16" version="1">ISSDLIQCGLTVASIRLIRKRKTGASRGFAFVEFNSIQEAKSWMDYKQGVLLLHDQNRAVMHYSHAKEPLTSKERTLTDWYCAKVRFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEW6</accession>
    <name>T1GEW6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEW6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001889-RA">
        <property type="protein sequence ID" value="MESCA001889-PA"/>
        <property type="gene ID" value="MESCA001889"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1284602_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001889-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="24942" checksum="FEEB49C1D991EEA1" modified="2013-10-16" version="1">MFSLRKSNFLFLNFENLLLIAARKTYQRKQSSFALANNNRKARTTTTKPLKLLRNGNSTFSDWNQSHVVSKRSWSSSRIMESGSAVSGNHFNIQDYDIIGFDLDCTLLRRKISRNERIFKVATFRKYVLLCFGWVFDFIQCCCISMIALGSGSTTKWERNGNKYKFRETRNVDDSDSRLLRHKQEGTHKKKRVGYLLSLLNLVDLTPSLELCDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG20</accession>
    <name>T1GG20_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Caspase family p20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG20"/>
    <dbReference type="STRING" id="36166.T1GG20"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002325-RA">
        <property type="protein sequence ID" value="MESCA002325-PA"/>
        <property type="gene ID" value="MESCA002325"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_785937_0_0_1"/>
    <dbReference type="OMA" id="QEPPINV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052039">
        <property type="entry name" value="Caspase-related_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576:SF41">
        <property type="entry name" value="CASPASE 14, APOPTOSIS-RELATED CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576">
        <property type="entry name" value="MUCOSA ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1/PARACASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577320" description="Caspase family p20 domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="5">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="4">
        <location>
            <begin position="276"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002325-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40161" checksum="95724CEF478695C9" modified="2013-10-16" version="1">MYSRPLEKIAIVSILLSLIESTSIKSQPNARAAMSFQTTRQSETKKTSVFVTSNCIERQTITTTVSSKIDRRVSFSTMETEPMFLRSPERATIAYKPESKMLAYKSDPKIKVLQTSRRPSLKSDAYMYQKASENPPLPNIVNANLKNGPVYVFSNSKIDKNKNVINCYDKDINSLNNAFKKFNMNVIVKKDKTKSRIQNRIKEKFMYCCGNFVGGQRNDKIEAKDDLYSIDDDIVLPLLKNKTLQGKPKIFIIQASRGDMEISVKTDGVSYGGNPSEVIKLYSSFEGYKSYRNEEGSILIQEFVNVLNESGDSLGISDIFEHVIKEVRFKTRGKQIPVMVKNLDQRYVFGQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGJ5</accession>
    <name>T1GGJ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02390942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02390944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002516-RA">
        <property type="protein sequence ID" value="MESCA002516-PA"/>
        <property type="gene ID" value="MESCA002516"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1519584_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002516-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="20536" checksum="365C2789A39811D7" modified="2013-10-16" version="1">MDTKRFMTGKSTTNQTDPYYEYNSKKAFFLPFRYLRRGGAAQPPPRDTLSTKRNLPEGCRYKSKNYNHSSIKTTDSGGPKYHVGRDLYCLACSIKLKKKMFFKSSAIWNLLVTEHRSSRSKSCSVHRKDKTSRSALIVIELVFLIGIDFVIFIFYTVYTSVFKRIEDARKSKSSFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGU4</accession>
    <name>T1GGU4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Metalloendopeptidase</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CAQQ02142139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGU4"/>
    <dbReference type="STRING" id="36166.T1GGU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002625-RA">
        <property type="protein sequence ID" value="MESCA002625-PA"/>
        <property type="gene ID" value="MESCA002625"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_788215_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6 7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="9">
        <location>
            <begin position="154"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002625-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="352" mass="41037" checksum="6A6FDF64D4C45220" modified="2013-10-16" version="1">MIRSPTIKITDVTKPDLKAVYECKRSPLQTVNLTGFNPVVGDDINSQTLAKPCMEVLKSTATKILLMQKPQRPSVWKDKEYDEATLPEKCSKKHESNEGSVSKQKEGDKLLAMNIFLIVLLGVVPCYLCLEFKDGDILLEDMILTQNQYKWLLNSTDRSGSDPYKPWPRATIYYKFYGKFEDIMKALKKIMKVSCLKFVDGYASYGLGQHLKIRKDDKRCYSGVGFRGTLFPMSIFLGKCDEGGIIHEFLHIAGLKHEHNTQIRDNHIKINWKNVMLNKIMNLDKYTIDTTFGLPYDFDSIMHYHSYVFSKNRYPTIIRKNNKKRYITRNKKMKLFMVFKYDRERLKCYFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH03</accession>
    <name>T1GH03_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02393823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH03"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002685-RA">
        <property type="protein sequence ID" value="MESCA002685-PA"/>
        <property type="gene ID" value="MESCA002685"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1126911_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002685-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="247" mass="26804" checksum="1F16B5C30B572FFC" modified="2013-10-16" version="1">MNYQIQFLIDIPIQHVSNLFLSRCEMSSRRTKPPHEILGLGVGVKEESAGSNCNVCPHVIAAEENQNKQSQCSSMRPTATNNGGGKGDTAGLGVSGRRISLKSCSDSSLDGRSSDSTYTNSSGGFECCGCGAPAGLRWRNNPNATTKLPIVEKLNINGTGISRPSYRNPYDEMTNFTNKSHINLNSTYPKLSHSFYHRKQSKPGGRTYPSQDISKEICENGLSTTVSGTISTNGDGKRFVNVEKFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH54</accession>
    <name>T1GH54_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02192219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GH54"/>
    <dbReference type="STRING" id="36166.T1GH54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002739-RA">
        <property type="protein sequence ID" value="MESCA002739-PA"/>
        <property type="gene ID" value="MESCA002739"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1588357_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF3">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="76"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002739-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="168" mass="19418" checksum="BCDA6A8AFFDDF6C0" modified="2013-10-16" version="1">MDAKPQYFDFAVPESNDGNLVSDNVFLTIVDQKEQEDDSGNCQFIVVRNVEPKSSGTVSRKQNQINRLVDIKREEKRRVTHNEVERRRRVKINNWISQLKELMDGKSSNVDSHHTSDSKSQILVKACDYIKDINMQLEKLTEENTKLREENALLHNKLLESSSIDNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHJ5</accession>
    <name>T1GHJ5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Nonsense-mediated mRNA decay factor SMG8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Probable component of kinase complex containing nonC and recruited to stalled ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SMG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394002">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHJ5"/>
    <dbReference type="STRING" id="36166.T1GHJ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002899-RA">
        <property type="protein sequence ID" value="MESCA002899-PA"/>
        <property type="gene ID" value="MESCA002899"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008116_0_0_1"/>
    <dbReference type="OMA" id="FATHICH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019354">
        <property type="entry name" value="SMG8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13091">
        <property type="entry name" value="AMPLIFIED IN BREAST CANCER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13091:SF0">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR SMG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10220">
        <property type="entry name" value="Smg8_Smg9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002899-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="776" mass="87910" checksum="92823A925EB7FE7A" modified="2013-10-16" version="1">MGRYKSCIYPNLTSEFESLLSSEDPNLIIVGVIGKSSFDDANKLAGFGILDVAPSLLSHQPKEGQISFYHKPGEQILFVHFETTFDNYCVEKMLNEWMTNGQFEQFMAFNSEVKTRFARSLLFATHICHIIVLVETGNNFDHSYLSLFKSLKVLREKHVAKFLPKMTKAANLPQSLGKETRLCCPRFIFFFESFPDEDGNGVTKEDISKFEFQVEDNFIKYLSDVFVEKKREFMPLLQDYVREALEVGFDDSLSKFKGKSHFVILPNKQWYQTFSFFHKIFITNADDSTLETSDLEYKTFLETFPTIVNVDFDFFASCCDPGLAKAISHYNSSNAPHYGFGYHKVLLDEALNVYRKNFLSQEKQHINERIIRDTCENIWRQGRQQCEFLSLRNNPCILPKHNAHDLAEHSSGVVFVSTCNCGRIQGRREDPYTLYQANFEFYQILYKNCGSCQKSLKIDVPVFTPSISDYRAAAVAESLANMNINQADESQYFLVSENAINRIDEDSSDASLNDMEPVEHQAKAPGESIYRQPSTTEYLPGMVHNESPQGLLPQFSSWSLLCVGPSSLYSHSTGLLEHFQSGFLSGANYLLPWDVQVRVKNPANFENKGNERRGAYRKKGAIMLKIFVGCEYECPRGHRFIMSSPDTVLNGGSGIVKDSGSKIVNNDMPLYFACPCRTSKNMVAQLMRVHVVTPKANVNVIMEPKVRTGERNLIFSMGLTSSAKLSQSAYWILRLPYIYQGEEGPMPPPAEISTSNAGVYGVLLGGMFKVVEGDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHP0</accession>
    <name>T1GHP0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02126367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHP0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002948-RA">
        <property type="protein sequence ID" value="MESCA002948-PA"/>
        <property type="gene ID" value="MESCA002948"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530050_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002948-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9613" checksum="1261CF72DA5DB542" modified="2013-10-16" version="1">MEHTTPATSPATESVRGAQRNYTELYPVKEVFSATLEETKYPIRQAYTKQTWNHPKLILQKLGRPCYRAHMAIKNTLSYLKSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GII4</accession>
    <name>T1GII4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02056873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02056876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GII4"/>
    <dbReference type="STRING" id="36166.T1GII4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003263-RA">
        <property type="protein sequence ID" value="MESCA003263-PA"/>
        <property type="gene ID" value="MESCA003263"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451118_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="2">
        <location>
            <begin position="42"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="2">
        <location>
            <begin position="115"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003263-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="187" mass="21306" checksum="64C744877FF09F5A" modified="2013-10-16" version="1">MASNYKGNGNRRRQDNNNRQVSGYTKQQGGGYNNQQQGNGNYNNRNQQVDDIQNLTYNLCHLFPRCNRAVSYPAPAYLAHHAATRGKVFIKNMGNFDNLEDLNRTIIGVKMNKAYKPKVTCIIVQKRNHTRFFPLEDGPSFGKMMNVFPGTVVDKVICHPKMKEFFLGSHESGFGTARPTKYIVIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIY1</accession>
    <name>T1GIY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoenolpyruvate carboxykinase (GTP)</fullName>
            <ecNumber evidence="3">4.1.1.32</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02183864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIY1"/>
    <dbReference type="STRING" id="36166.T1GIY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003418-RA">
        <property type="protein sequence ID" value="MESCA003418-PA"/>
        <property type="gene ID" value="MESCA003418"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144503_0_0_1"/>
    <dbReference type="OMA" id="KIASWPC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071333">
        <property type="term" value="P:cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046327">
        <property type="term" value="P:glycerol biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019543">
        <property type="term" value="P:propionate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase C-terminal P-loop" evidence="10">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003418-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="12934" checksum="816971CB31E362F2" modified="2013-10-16" version="1">MRIMTRMGTPVLEQLKKKEEFVRALHSVGTPANGVPKIASWPCDPERTIILHKPEENLIVSYGSGYGGNSLLGKKCFALRIGSTIAKREGWLAEHMLILGITDPKGVKKYITAAFPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJB7</accession>
    <name>T1GJB7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02037216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02037220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJB7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003562-RA">
        <property type="protein sequence ID" value="MESCA003562-PA"/>
        <property type="gene ID" value="MESCA003562"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075846_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003562-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="118" mass="12482" checksum="EDD61B196D4029EA" modified="2013-10-16" version="1">MKKISSMAINQLNKQNAENDMNSNNCYGTISESDVAGSGLTMDQPQPQPPQNVYSNILKEFSNKNLNGNDDTTTDGVQFPIQGNNGATWIGGSGNGTGSGFEYVTYHGLVGGFNLGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJY6</accession>
    <name>T1GJY6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02120742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02120743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJY6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003796-RA">
        <property type="protein sequence ID" value="MESCA003796-PA"/>
        <property type="gene ID" value="MESCA003796"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798648_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003796-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16383" checksum="57E9E83EE6D616DE" modified="2013-10-16" version="1">MSNPKKPASSKAIVDNSAKLLPQKKSIGAASKESVLLKNTRGNDKGSYHLYRQKNQRLKQQRRGSFITVSANRNKDLDQHNSARIIKDSLFFDIIIFRGETRRPKIAHITYEIDFIGRTSSEIIDRFLAIEKVANSVELKVNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-11-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK43</accession>
    <name>T1GK43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">40S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK43"/>
    <dbReference type="STRING" id="36166.T1GK43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003858-RA">
        <property type="protein sequence ID" value="MESCA003858-PA"/>
        <property type="gene ID" value="MESCA003858"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2457348_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003858-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="10273" checksum="3FEAFA02AB468A2C" modified="2013-10-16" version="1">MLLICRYVTHLMGRLRHSQVRGISIKLQEEERERRDNYVPAVSALEQDIIEVDSDTKEIIIVKGSNFSYLNGKFIVKSGSMFMLSIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKS7</accession>
    <name>T1GKS7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02122498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKS7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004105-RA">
        <property type="protein sequence ID" value="MESCA004105-PA"/>
        <property type="gene ID" value="MESCA004105"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2352595_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004105-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="97" mass="10702" checksum="1DD6C3F511BF1828" modified="2013-10-16" version="1">MYGDGYGHHHHHHHRPRVEVDYNISLGGGYRPGYYPPAQTTVVYNQPAVVQPAGVYVPPVVAPSYPGTTVVTQTTAPAPGMIFCETRRRWVPSVEFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLQ1</accession>
    <name>T1GLQ1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLQ1"/>
    <dbReference type="STRING" id="36166.T1GLQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004464-RA">
        <property type="protein sequence ID" value="MESCA004464-PA"/>
        <property type="gene ID" value="MESCA004464"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1385615_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004464-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22345" checksum="D387B1202CEC769F" modified="2013-10-16" version="1">MKDTQYGLSGALLTLREYDECFLKRSTESMGRMEIRPRANFYLYVEVQFISGNSPSGGQIWYHVEIRLPLEWGDFPLKSGISPQDSSGANSPLKIVESEFPPDRANFKFPSLILALCSSQKRYYKSQGWALSIIYVVSNISVGVVATDIWIEDIRTFVAEFPFLEPRTIADAILYALNTPPNVEITEFTIKPLGERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLQ8</accession>
    <name>T1GLQ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02026850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004471-RA">
        <property type="protein sequence ID" value="MESCA004471-PA"/>
        <property type="gene ID" value="MESCA004471"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2707640_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004471-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="8558" checksum="3497D86B2B3CE1A1" modified="2013-10-16" version="1">MLFIDLSRIDLVESVELPKTVGINFNLIDQQPTMWSNPSCVWNMLQNPWAYRRKGFCRPISELSKAFKDLIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLX5</accession>
    <name>T1GLX5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABI family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02183720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLX5"/>
    <dbReference type="STRING" id="36166.T1GLX5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004541-RA">
        <property type="protein sequence ID" value="MESCA004541-PA"/>
        <property type="gene ID" value="MESCA004541"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035421_0_0_1"/>
    <dbReference type="OMA" id="YCEGNYF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11826">
        <property type="entry name" value="SH3_Abi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000002">
        <property type="entry name" value="abl interactor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028455">
        <property type="entry name" value="ABI3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012849">
        <property type="entry name" value="Abl-interactor_HHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF0">
        <property type="entry name" value="ABELSON INTERACTING PROTEIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07815">
        <property type="entry name" value="Abi_HHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="71"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="418"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="176"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="364"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004541-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="52576" checksum="7418252C897326A6" modified="2013-10-16" version="1">MNRYSYNTESSYQDHHSVMANENIMDELASLIRTEIPEGRQTLRDSFTNLERVADYCEETYLRADNASKKEALEHTKNYTTQSLASVAYQINTLAYSFMQLLDLQAQQLGEMESQMNHIAQTVMIHKEKVARREIGVLTANKHRQYKIVAPINPEKPIKYVRKAIDYSILDEIGHGVNSGPNRHKPRGSNQGSIQSLANPQMMTSGPPPTTKPPTPPQAGVIGGRQIISGTGTLGKMAGNTGTLGKASREYRTPPVVNPPQVPSHYAPNYPIGHPKRVSISSAGDRGPGYSALPIPPSQQIATHVNLPSAGMMQNIPPPPPPTNSYDGTNRMPPPPSPLAVNNLEINEQSHIGMHTLGRHHNISHNFIRPGSQSPPLPPPPPPEDEHQDFGRPRTSTQGQLAPIVPDDQNLPGWVPKNYIEKVVAIYDYYADKEDELSFQESSVLYVLKKNDDGWWEGVMDGVTGLFPGNYVEPCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM43</accession>
    <name>T1GM43_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metalloendopeptidase</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CAQQ02199961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM43"/>
    <dbReference type="STRING" id="36166.T1GM43"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004611-RA">
        <property type="protein sequence ID" value="MESCA004611-PA"/>
        <property type="gene ID" value="MESCA004611"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_500882_0_0_1"/>
    <dbReference type="OMA" id="KQSELWD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="12">
        <location>
            <begin position="111"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="12">
        <location>
            <begin position="365"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004611-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="544" mass="61723" checksum="E510FA7A41EFC9EC" modified="2013-10-16" version="1">MKDVNCEQKLRTPDYSKLINTFPCPVTFNLKSTLQARCTKLFQKPVEDLLTRSIAIMKPYILIAALFFGTVLTQSNEDPAEDSAQPELTEGDMILTEEQKQILFFGSDKRNGVTNPSLRWPNGVVFYQFTGNIPNNHRNLIINSLREIERSTCVRFRQGSNGNRYIRITNNAAGCFAYVGYLKSAQQLNLGNGCMHKGVIIHEFLHAIGFYHQQSSADRDSFVNIHLENVQSGQEHNFNKYSSSQVTNFGIRYDYGSIMHYGPYAFSKNNRPTITAKFSGGENMGQSNGLSQSDILKIRRITMKSHFIVSLLAAIAFAQPIDEVKKGDGPKPPKKPWPTDIPDLTEGDMILTAEQKDFLFSETRNGVTDTRLRWPGAFVYFWFDSNFSNDERNLVISALIEIQKYTCVRFVMGANSENHYIRVTKSSSGCFAYVGYLRAAQQLNLGNGCMHFGIIIHEFLHALGFYHEQSTYNRDDYVYIHLENVISGQEHNFNKYTNHKYPTLELDMITVASCIMEPIRSVKMDGLLLLPSTVVDKIWANGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM57</accession>
    <name>T1GM57_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02192130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192131">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02192132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM57"/>
    <dbReference type="STRING" id="36166.T1GM57"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004625-RA">
        <property type="protein sequence ID" value="MESCA004625-PA"/>
        <property type="gene ID" value="MESCA004625"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220903_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF853">
        <property type="entry name" value="GH25466P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004625-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="227" mass="25491" checksum="ABE8CDB8DA46AF80" modified="2013-10-16" version="1">MCVKHNVQRLIYTSCASVAFTPFKGHSTFSVVINQTETSASTPVHDPNRPLNDFDKSFLIPGYSSSKLRAENMVLNTNETLLENRKGNAFYYLQTAAVRPPLIYGECDNHFMPAVLKYLAKRNNIIPRVAGAGGKQQLVYAGNAAWGHICTYKTLKSNPRKIAGLAAFITDDTPINDICRFIQRIGRTSKHLKFNTSLWSIPHFVFFFLAFALEMFVKLLHQLANSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM58</accession>
    <name>T1GM58_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02134213">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM58"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004626-RA">
        <property type="protein sequence ID" value="MESCA004626-PA"/>
        <property type="gene ID" value="MESCA004626"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123892_0_0_1"/>
    <dbReference type="OMA" id="RETHRIK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004626-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="13642" checksum="5D67FB1F5BDD31FD" modified="2013-10-16" version="1">MFRFEAYELPMYMYSRVVKRIQQHSTTCKDPKHKDKSSLADHHHSLSHNFDFQNFKILDFEPNHVKRRISEMIYITMQGENKVNVRSDTENLSTSYKNLIEKSNKNRDSNRSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMB3</accession>
    <name>T1GMB3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMB3"/>
    <dbReference type="STRING" id="36166.T1GMB3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004683-RA">
        <property type="protein sequence ID" value="MESCA004683-PA"/>
        <property type="gene ID" value="MESCA004683"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193219_0_0_1"/>
    <dbReference type="OMA" id="AILIIME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004683-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6970" checksum="72600B8223EAA2D6" modified="2013-10-16" version="1">MVMRQGLHGPEYYGGIILFFIFGAWAGLTVGILVMMEGLSAFLHTLRLHWVEFMSKFYQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMI0</accession>
    <name>T1GMI0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dystrophin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02045283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMI0"/>
    <dbReference type="STRING" id="36166.T1GMI0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004754-RA">
        <property type="protein sequence ID" value="MESCA004754-PA"/>
        <property type="gene ID" value="MESCA004754"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1697535_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF14">
        <property type="entry name" value="DYSTROPHIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004754-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="16612" checksum="90D4B78D3CB6A811" modified="2013-10-16" version="1">MMAEAKLLRQHKGRLEARMQILEDHNRQLEAQLQRLRQLLDEPNNAPGKPSTLQTRSVTASQLNTESPAKMQQNGHYEQNSNGIVGVNASGNHGVGDLKHNGPSIGATGMQMNLVGGVGVPGNPNSLIGNNSHLMNDDGRPPPPPHSSLLHMAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMU9</accession>
    <name>T1GMU9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02191854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMU9"/>
    <dbReference type="STRING" id="36166.T1GMU9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004882-RA">
        <property type="protein sequence ID" value="MESCA004882-PA"/>
        <property type="gene ID" value="MESCA004882"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017344_2_0_1"/>
    <dbReference type="OMA" id="VPQLHIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="27"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004882-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="173" mass="19444" checksum="C53153C4EC9DAC30" modified="2013-10-16" version="1">MNHYEYPSMVKTLGKFKLTVQEGHFSDSEILVLLGENGTGKTTFIRMLAGNLEPDEGSGKVPQLHISYKPQKISPKSQGNVRQLLHDKIRDAYVHPQFIADVLKPMKIEEIMDQEVQNLSGGELQRVALTICLGKPADVYLIDEPSAYLDSEQRLVAARSSKDTFCMQRKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMW4</accession>
    <name>T1GMW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02192992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMW4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004898-RA">
        <property type="protein sequence ID" value="MESCA004898-PA"/>
        <property type="gene ID" value="MESCA004898"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107288_3_1_1"/>
    <dbReference type="OMA" id="NIKCFAN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857:SF43">
        <property type="entry name" value="GEO07291P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857">
        <property type="entry name" value="ODORANT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00708">
        <property type="entry name" value="PhBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577529" evidence="5">
        <location>
            <begin position="17"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004898-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14516" checksum="40DD8E25A7AF8AAE" modified="2013-10-16" version="1">MKAFLVFAVILAVAVAEMHEMMGEQFKKECMDQEKVDASELEKVRSGQLDNPSEKLKCFNRCMFEKGGFFKDGMFVDEKVKEFLNKHTQKEHMLTAYEDCKKIKGTNDCDTTFKVFECMHKAHMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNM7</accession>
    <name>T1GNM7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nose resistant-to-fluoxetine protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02083992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02083994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNM7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005185-RA">
        <property type="protein sequence ID" value="MESCA005185-PA"/>
        <property type="gene ID" value="MESCA005185"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007874_2_1_1"/>
    <dbReference type="OMA" id="TADREPY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577047" description="Nose resistant-to-fluoxetine protein N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="582"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="3">
        <location>
            <begin position="39"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005185-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="632" mass="72049" checksum="9BD68292CC2B57F4" modified="2013-10-16" version="1">MKSYWALILCLCQLVSSFDDFTTFQKPQEVLYQDRFIDDDLCDIQWKEIRTNNDKYFQIYDSWGKTPSGILKGHTTRRSKRRNFQGQYCLALIPLETIQSISSSLAGPVRTARAGGEIFQKDQTKIKLGICVPAACSEAKVSEKLGVDLRKNSCSIKNQYKFEAIDYVAIVLFSTIGLLIVCSTLYDYYPHQSETKLTQALLAFSLRTNVKKLVSSPKKTSSSVDCLNGLRVFSMFWVIFGHIYMWNLGLPLMNFLDIPNWKETPFSMIIQSATVSVDTFLFISGFLIAWLGLKEMEKTKGKLNILSMYIHRYIRLTPAVAAAVLFVVSLLKFMGSGPYWHNFLDVHKKGCDEYWWSTLLYVQNYCLGHSWYLAVDTQLYILSPFILIGLWKWGKKFLPAMLIMALLSIGCIFATFYTEGFNQIGSRTKIKANYVPTHTRYTSWLIGVAVGYVLYSNRFIKFRLPKWAVFLGWAVCMATMLAVIFGPIHTTVPGYKPSVTEAVFYETFSRISWCIAIGWIVFACQSGYGGPINSILSFSIWAPFCRLTYCMYIVHFVVQTTIFGNLKTDVTFSDFNAIQEFWSSFGLTFCVAIVFVLAFESPIIGLEKVFIRKEKTRSPAMSTTSSQRALEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNZ2</accession>
    <name>T1GNZ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02043384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNZ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005305-RA">
        <property type="protein sequence ID" value="MESCA005305-PA"/>
        <property type="gene ID" value="MESCA005305"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476796_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588434" evidence="1">
        <location>
            <begin position="23"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005305-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="21220" checksum="CDF155EE328F69DB" modified="2013-10-16" version="1">MSFSKVLIFSFAFLIIGNFVKSLKDFEKCNVGKNSITTNLQNLDFNCPKHNEIFRIKWFMRNYYPYFVVKEGQKHIEMKNTGDSFRIIEDGKACDSIPTAKKILNNFHFTELEVALRKDGKLMFIAHGSAGFSLVCDLKQKFSGNDIILQIGYGSQVLDYNFYYDCPFRDSTFKSQSDRDFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP46</accession>
    <name>T1GP46_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02063708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02063709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP46"/>
    <dbReference type="STRING" id="36166.T1GP46"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005363-RA">
        <property type="protein sequence ID" value="MESCA005363-PA"/>
        <property type="gene ID" value="MESCA005363"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3191870_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005363-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="46" mass="5662" checksum="4D86FF241C724314" modified="2013-10-16" version="1">MPRGRLEYLKRLIPYLKNQSEDCLYLNIYVPIQENMLKLWFSTQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP71</accession>
    <name>T1GP71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005388-RA">
        <property type="protein sequence ID" value="MESCA005388-PA"/>
        <property type="gene ID" value="MESCA005388"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1148346_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005388-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="242" mass="25767" checksum="309F9E352C1A1554" modified="2013-10-16" version="1">MDLYLTEILYCCCSFGFGLCRVSHLSQNYLPPTHHHEHPGYSYEPPHAASSFQPQYQPAPAPTFTQVAESRPVPQYTAPAPAPVGAGAGAVYCGTAPAPVQTFSQVAESRPVPQYTAPAPTFTQTAETQTVQQFTAPTQSFSQQSTNVAPTYNQPEFRGPQTFTQASTNVVPQYTAPAQTQSFVSAPAPVAPAAPQFQPQASAPAPEVIIPSQSDYEAQPTIISGNTGSLPTTDIITPGRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPL6</accession>
    <name>T1GPL6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4794 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02182277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02182279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPL6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005550-RA">
        <property type="protein sequence ID" value="MESCA005550-PA"/>
        <property type="gene ID" value="MESCA005550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1176446_0_0_1"/>
    <dbReference type="OMA" id="KYMPYIV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588444" description="DUF4794 domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23996" checksum="A8D793E103BE7B2E" modified="2013-10-16" version="1">MNVGYLLVSLASLTTAIRVDWNTNQGPIVPPIPTVQPVPQPPFRSPAPVWEDQSNDIPNPNPYQYVLPPPSRPKYPDNNYLTAPLYPNRQTAYQVVPIPATAINSLYYNYKNNIVPSLGIQYIPNIGNRYTYVIPSSGTATASTTTTTTTSASKSGNYIKPDKLNGKYNPKLKKYKANRDSNYSRIFFKAIWLYISKTVLMELPTLPWERHDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPV2</accession>
    <name>T1GPV2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPV2"/>
    <dbReference type="STRING" id="36166.T1GPV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005646-RA">
        <property type="protein sequence ID" value="MESCA005646-PA"/>
        <property type="gene ID" value="MESCA005646"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1242804_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005646-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25410" checksum="AEFE4AEFD49AA84F" modified="2013-10-16" version="1">QKSRSSVESVDSTILYVASAGKNALDKNYRIVATVENFFEIIYNVHVELGGRNGRHAGQKRTYRIISETYAFLPREAVTKFLSICPACYKNLRQNSPCSETSTFYSANNSITSLAPSTPVESNKLEPFQNSENFDLHRNFLKQFTKLQEIVKKKNIENVETTIDFQTDEKLSIFRSPLKKELDLTKVKPITSVYLQLTRSMGLSDSDALQFNDFVSSDFKYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ00</accession>
    <name>T1GQ00_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 39</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02115274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02115277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ00"/>
    <dbReference type="STRING" id="36166.T1GQ00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005696-RA">
        <property type="protein sequence ID" value="MESCA005696-PA"/>
        <property type="gene ID" value="MESCA005696"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008826_3_0_1"/>
    <dbReference type="OMA" id="RMQRIQK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037386">
        <property type="entry name" value="CCDC40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16275">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16275:SF8">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="693"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="237"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="567"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005696-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="88302" checksum="71476151C71EF2F1" modified="2013-10-16" version="1">KVCEERTRIEKEVVPLQKDKETLDEELQRYRQVLKRKRMDLDNYNTLELTVHKWAKEVEDELRVTKTKAGKDAKIQKQLSEEKKKSDFIVFNLEMEILKRQKELDELRDQVNNQKQIIETLNGSYYDASSDLDALQNEHKRITQAWKEVIICIQHRDKVLYKGKEDQEKLKEDLKLTMSSIQAVKKLIKKVEDEGKVINCFKSRLEEDFKIQNRNLEKELEIQAKVDAKLDEIPILIEQTEKDFTEAKKESTQISKELREVMAKVDKWAMEKVKLEEDFFKEAQEHIITDNASSYSIRLLKDSQRTRRNMELNLAATENSLSEVLLELERNKGLIQKLTLNKEKLEEEKVLILVDDARTYSIYEERQNSQYKESTIARKELLIITQKSIIIDKELEDAQACGRQIVAEIGRFEAKFDKLGSSLFKQKTTHEKEEVDYYHKHSELIANLKEKETSCCQSQYEIEEIKKEIEDLKLEVLEHHREALAWETKYKVIEDTLKWLKEEKSKDGEINQMKQEIRRMEVRYHQLKRAQEKLVQDLQHSVVHRAQIYEQSEMKNKLEKSGSLRIRSVVQSRINELKSKMKKIQSEFFRTHKALAEVTEEIRQIVEKISRKEDEIEREQITQNLLNVEIEQAMLIKYQNLESIVRKQNRAKANKKLITGTVLPKLKSEALINAFTPVKCFTSINIEDKPDEEALSKRMRSEDISSENLTKKQTKLEGSSAKLRLFVLNKKANEFRIRQDQWVQTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ66</accession>
    <name>T1GQ66_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02051360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ66"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005767-RA">
        <property type="protein sequence ID" value="MESCA005767-PA"/>
        <property type="gene ID" value="MESCA005767"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530035_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005767-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9317" checksum="84AB75E732E10DBB" modified="2013-10-16" version="1">MNGKETFSKVQRLAAILSKMWKKLELFGAASTSCLGCCSSRSSAEVWLLPAKIVRYFVMLFIQSGNCHTESQGSRNNHVKDSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ89</accession>
    <name>T1GQ89_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02041416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005793-RA">
        <property type="protein sequence ID" value="MESCA005793-PA"/>
        <property type="gene ID" value="MESCA005793"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387338_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005793-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22316" checksum="A3CF720E39854526" modified="2013-10-16" version="1">MDAPLSKSPSICLPPISTLANFRVSHVRGGVVTPTFLFSPKNKEYLICRESFRSPSYESLDKYNLALESFNLSDVPRGCIPYSLVTPNYCNDWYYVKNNTPPKSPTKPQSSITPQPQIKFRSTKKSPSMSSINMAPAFNKISAPNEITKHEHYKQPNNLKTKETTGNEYFTCPVNGNNFMYNQIGIVNEQQYTKDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQC8</accession>
    <name>T1GQC8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02023202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02023205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQC8"/>
    <dbReference type="STRING" id="36166.T1GQC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005832-RA">
        <property type="protein sequence ID" value="MESCA005832-PA"/>
        <property type="gene ID" value="MESCA005832"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010982_0_1_1"/>
    <dbReference type="OMA" id="EMNLMEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16196">
        <property type="entry name" value="EFh_PEF_CalpA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000241">
        <property type="entry name" value="Calpain-A, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF433">
        <property type="entry name" value="CALPAIN-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="10">
        <location>
            <begin position="198"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="812"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005832-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="911" mass="103584" checksum="236B64B5683C3DC5" modified="2013-10-16" version="1">MDELKGFLKQTGREFLNAAGTAFTDAAKDVVGNVINEIFVKKESETKRFLPSIKNMRVKKNNFVVPEEVQIPSITVSLNSSRVDLEPDLIEEVLQKLDLTKQENVANLTKTHFYNSVVNLAEKSPIFPHIDDEKDKKSLLNLKIQSETSKENEKPKRKMTFDGLPIYNLGERASGLRPHGEVQDYSLIKQQCLSSGSLFEDPLFPATDESLMFSRRPDRNIEWLRPSEISDDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSNLFFRVVPVDNSFEDGYCGVFHFCFWQYGKWIDVVIDDRLPTALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYELKEAPPNLFNILAKGMERNSMMGCSIEADPYVTEAETPQGLIRGHAYSITKVQLMDIVTPRTSGKIPMIRLRNPWGNEAEWNGPWSDQSPEWRYIQEHVKMEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEEQQNSGKRKWEMSVFEGEWTSGVTAGGCRNFLDTFWSNPQYIVSLEDPDEEDDDGKCTIICALMQKNRRSKRNMGMECLTIGFAIYHLTERDMYTKPQPVSFFKYRASVARSPHFINTREVSAQGEFLIRVFSETKNHMEENDDHVGYGDVDDRVTPSLPMPKPADPQRAALERLFRDIAGPDMEVDWVELKRILDHSMRDGKVFNTCADIFVWKLSKYLPRAGSVMNGINQQPVNATMTDDGGGGNLFALLCGPFCKGTPFEEMLGLNEQNQPQPNGNISNVHPITSQVLYESGGFSKDVCRSMVAMLDADQSGKLGFEEFEALLTDIAKWKAVFKLYDKEGSGRMQAFELRQALNSAGYRLNNRILNALAHRYGSRDGTIAFDDFIMCAVKIKTYIDIFKERDTEGTNTATFSMDDWIEKTIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD1</accession>
    <name>T1GQD1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02082802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQD1"/>
    <dbReference type="STRING" id="36166.T1GQD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005835-RA">
        <property type="protein sequence ID" value="MESCA005835-PA"/>
        <property type="gene ID" value="MESCA005835"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033484_3_0_1"/>
    <dbReference type="OMA" id="RYENSCY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002031">
        <property type="term" value="P:G protein-coupled receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000020">
        <property type="entry name" value="Arrestin, Arr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000698">
        <property type="entry name" value="Arrestin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014753">
        <property type="entry name" value="Arrestin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792">
        <property type="entry name" value="ARRESTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792:SF23">
        <property type="entry name" value="PHOSRESTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00309">
        <property type="entry name" value="ARRESTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3" id="KW-0844">Vision</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="123"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005835-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="308" mass="34337" checksum="12211C93AE11E88B" modified="2013-10-16" version="1">MGVKFSKEMILCTDQIVPLVNQKMEMTPTQEKLVRKLGSNAYPFTFHFPQASPSSVVLQQEGYEGSKPLGIEYSIRAFVADNEDDRQHKRSMVTLVIKKLQYAPSNRGQRLPSSVVSKGFTFSNGKISLEVTLDREIYYHGEKVGATCHITNNSKKTVKNIKLLIVQHTEISMLNTQFSKHVASMETKEGCPITPGANLTKCFFMVPLASSNKDKHGVALDGHLKDEDVNLASSTMVQEGKTMSDAIGIIISYTVRVKLNLGTLGGEIQTDVPFKLMTPAPDHYKDDDDNIVFEDFAKMRMANVHMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS8</accession>
    <name>T1GQS8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS8"/>
    <dbReference type="STRING" id="36166.T1GQS8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005993-RA">
        <property type="protein sequence ID" value="MESCA005993-PA"/>
        <property type="gene ID" value="MESCA005993"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485131_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028641">
        <property type="entry name" value="RCC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46207">
        <property type="entry name" value="PROTEIN RCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46207:SF1">
        <property type="entry name" value="PROTEIN RCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005993-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9055" checksum="2315308DB5AE6E06" modified="2013-10-16" version="1">MAAYCWGSIINGPLGGVEEELLLYPREIDWSESAKVIKVSCGTSHTLFLTNDGKTYSCGNNDWGQLGHDLLPRKRPRMLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRE7</accession>
    <name>T1GRE7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynein heavy chain 3 AAA+ lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02165466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRE7"/>
    <dbReference type="STRING" id="36166.T1GRE7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006228-RA">
        <property type="protein sequence ID" value="MESCA006228-PA"/>
        <property type="gene ID" value="MESCA006228"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_728230_0_0_1"/>
    <dbReference type="OMA" id="VHECHRT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001221">
        <property type="entry name" value="Axonemal dynein heavy chain 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF4">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="2">
        <location>
            <begin position="294"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="380" mass="43585" checksum="59D94724229AD1DC" modified="2013-10-16" version="1">MKLEENEEVCSPEQLHRLYIFANKVECLYERNLSRLDYPSGDTENTIFDFFVSEAGAWQSWKSLVTPYLYPEVSTPDYLSILVPIVDNVRIDYLIGTIANQEKAVMLIGEQGTGKTVMMKNYMKKLSAETHMSRSFNFSSATSPYQFQKTIESYVEKRVGVTFGPPGGRKLVVFIDDINLPEINCWGDQITNEIVRQTMDMKGFYSLEKPGDFTTVVDVQYVGAMGLPGGGRNDIPSRLKRQFCVFNCNIPDNDSIDKIFRVVGEGHYNAKRGFSTDVRSLVKKLIPLTRHLWEETRNKLLPTPAKFHYVFSLRDLSRIWQGMVGTLSTVITAEIVLMSLWKHEVTRVFADRFTTVEDKEWFGTELFALVKKELGEIIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRG2</accession>
    <name>T1GRG2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02045053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRG2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006246-RA">
        <property type="protein sequence ID" value="MESCA006246-PA"/>
        <property type="gene ID" value="MESCA006246"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_907860_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="307" mass="36257" checksum="18E770CAD7E93374" modified="2013-10-16" version="1">KYVPSSFLDVPSLAAVLPRPYKTNIYKNPSNMSNSCLLTSFLEMSLNNILNGSNSLESLKKLNLELVNTWKFSHVTSFNVNFENWSYERVENLLQSCLDFKLWRYACETEKFHTYQRSTLLLNTISLLRFTELWSFLKQNYYQHLEQYQYYRYQLGNVDANIINDITKILPLLQRRCVDLQIYIRETAIRIAYILNLQLYVLSMNKELKQESKLNLEKNHSFSNEYKSFVPFQNLANNKNDCITMIDTLWLDTFNKFYKPENSVQSFITLNAVNMAKKYSLDSKSSVKVLTKYKRVTNKIITLIQMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTY3</accession>
    <name>T1GTY3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTY3"/>
    <dbReference type="STRING" id="36166.T1GTY3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007182-RA">
        <property type="protein sequence ID" value="MESCA007182-PA"/>
        <property type="gene ID" value="MESCA007182"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2336003_0_0_1"/>
    <dbReference type="OMA" id="HRIMLFR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051591">
        <property type="entry name" value="UPF0224_FAM112_RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402:SF5">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007182-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11612" checksum="5D3D142400DC8DFA" modified="2013-10-16" version="1">MDNPLVQCPFDKAHKVLSHRLPIHIVKCKASYSGPEMEKCMYNATHYVPKGSLQTHYQSCKSFYEFHREVHEKFYEKHNIDENASEAFTEDDKSYYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTY4</accession>
    <name>T1GTY4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Farnesoic acid O-methyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02176454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTY4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007183-RA">
        <property type="protein sequence ID" value="MESCA007183-PA"/>
        <property type="gene ID" value="MESCA007183"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852280_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Farnesoic acid O-methyl transferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007183-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="65" mass="7482" checksum="1E087BF9A1CAD5DF" modified="2013-10-16" version="1">MDGVYFTEVELVINKEYGFVAVFLPNYGTSPFLKCYEPEAISKIKYIGFTGRHDTTARYIYDCPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTY9</accession>
    <name>T1GTY9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02015146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02015147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTY9"/>
    <dbReference type="STRING" id="36166.T1GTY9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007188-RA">
        <property type="protein sequence ID" value="MESCA007188-PA"/>
        <property type="gene ID" value="MESCA007188"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1512335_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09386">
        <property type="entry name" value="LIM1_LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF13">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="98"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="20232" checksum="3F6E07EE4A237DA6" modified="2013-10-16" version="1">MEDSCIKPNLIEDSSSLEKIDRRPPIDQAHISIALAIPPITFLTSWTFDADRNVIHHLLANPRMMIKEMEDIVKWDCACLGTFKIYHQNTILINNGSNALPSNDGKIMERYLLYALDRYWHNSCLKCYCCGATLGDIGSSCYTKAGMILCKPDYVRGGLIIFLTGEEHEIQEMMFHVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUB1</accession>
    <name>T1GUB1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BolA-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02097780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUB1"/>
    <dbReference type="STRING" id="36166.T1GUB1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007321-RA">
        <property type="protein sequence ID" value="MESCA007321-PA"/>
        <property type="gene ID" value="MESCA007321"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_3_0_1"/>
    <dbReference type="OMA" id="CLGGFGK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050961">
        <property type="entry name" value="BolA/IbaG_stress_morph_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229">
        <property type="entry name" value="BOLA TRANSCRIPTION REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229:SF2">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007321-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12436" checksum="0FED98D59E297F8D" modified="2013-10-16" version="1">MATEEKPIETAILKVLKEKLQPKHLEVINESYMHNVPKGAETHFKVYIVADEFESMTLIKRHRLVNGILKEALDWKFPHALSIDAKSTSQWDPNYATEKSPNCKGGFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUC7</accession>
    <name>T1GUC7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02047612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUC7"/>
    <dbReference type="STRING" id="36166.T1GUC7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007337-RA">
        <property type="protein sequence ID" value="MESCA007337-PA"/>
        <property type="gene ID" value="MESCA007337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576583_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF72">
        <property type="entry name" value="GAMMA-GLUTAMYL TRANSPEPTIDASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="8725" checksum="81C7697E85E5DDE8" modified="2013-10-16" version="1">MKRENLFDTSERVYKLFDSGATFILGKYVWNQRKDMGAIVSNGAKCGNIGKHIINEGGSAVDSIIAVLVCEGAILPHSVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUW5</accession>
    <name>T1GUW5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4704 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02110269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02110270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUW5"/>
    <dbReference type="STRING" id="36166.T1GUW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007537-RA">
        <property type="protein sequence ID" value="MESCA007537-PA"/>
        <property type="gene ID" value="MESCA007537"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002876_0_0_1"/>
    <dbReference type="OMA" id="ACKENIC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF112">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4704" evidence="2">
        <location>
            <begin position="490"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007537-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="899" mass="102181" checksum="8E66F5D2EA675D5C" modified="2013-10-16" version="1">MTRSSKKVIDSLRSSNKLNLIFTNIGNLGKPSMKVLEACFNFAFDDKLKTLLIPDSLCPIIKWLPDIDESNQLKVSEMILKGCTTNYICKQSSCEEEIIKIVCETLTSFEYLNEGCIQNLIKLIEELAKISIKPAELKGILSLLKEESKFPYTKQLLQTFTVVSLQNLNGGNTSCGQYFDIQKSYDGIIVPDIRNWIMSGSYGFIFHVFLRLNPLGEETGDSDTVKTTKRRMLFNLKTATDTGFEGFIQDNGNIVVAAVTKKEYLTSSVSAKTIFDGRWHFVTIALSPPKRPFSYSQINIYVDFIQKISTTLKMQGLNEPFTFCTIASAYQHHQRYQDHSPHSDRIARGSSQESSPQQFRGILPNILERTLSTHVSNYFTLPMRNTTSFDPNVKNFPIGMQDNVFGEPVCLRGQICSVLLAEPTTNMRTIFEAGTNFASLFSQDNDVIDLTSRFVFCYSAGSCTNNICTDLVPGGKYIGSVSARHFKIVKIQDTLNSVGGVQAILPILHNISKSSDFSLTSDVNEDDLLAAAQKTPSVEEFSDWEVLTTSSYTEWKMIQQPVAKTDTLAMIGVMLKKIPPSSFDVNALMGTHLLIESIQNQKPSANMKLLENLYLEIVFNFAIWSKMQFQIVLGHVQYISTMIKGDRKYFRKKFGVQYFLDVLRMYYGSPENINFEDSKTIRATIFAIIKYYIQKDVNIKEVSSIVAFMASVKYEHVLIELLEVLTYHMKSKSCKDDLILLMHEPQTANLFYNYFIDKVHTFELQEANIRFIETLLSSTRVQGKYKQILRLMEEESFYPGFFSYLIPLNLSESVLLRIYDQLLSSEIDYTGLMFLVYQVYQCDIPIKLEVAKKLLQVTFLKPTAPQSIAKRVGWQESVARLLIKKPIVDKLDVERGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVE3</accession>
    <name>T1GVE3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112592">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVE3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007740-RA">
        <property type="protein sequence ID" value="MESCA007740-PA"/>
        <property type="gene ID" value="MESCA007740"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_7_40_1"/>
    <dbReference type="OMA" id="LCWRVRL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038062">
        <property type="term" value="F:protein tyrosine kinase collagen receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF580">
        <property type="entry name" value="DISCOIDIN DOMAIN RECEPTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007740-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20475" checksum="7A4D33B4B5C2997D" modified="2013-10-16" version="1">MIAKKLSKVTNDNITKLLGACLKDEPICVVLDYRNCLGDLNQFLQESRNTLSFGSLVYISSQIASGMKHLEELEFVHKDLATRSCIIGPYLNVKVCSIGSVINRSAYSCDFCPIEGHSESMPIRWMAWESILLGKFTSKSDVWSFAVTLWEILKFSMDQPFEHLSDEKVLENISNIYHNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVJ9</accession>
    <name>T1GVJ9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02058663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02058665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007808-RA">
        <property type="protein sequence ID" value="MESCA007808-PA"/>
        <property type="gene ID" value="MESCA007808"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087556_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007808-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13560" checksum="209ED9EA96725208" modified="2013-10-16" version="1">MAFTKKHGIHRTKLTMFYAANVLDKKFKKQETIPADLDIESHCKFCAVWITVGSEESSKLKILRESNQTRKFYQGITKRRNNYTPSTLLCSDKSGKLITEKQEVLKNLFFSIIPNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVR2</accession>
    <name>T1GVR2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF753 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02026830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVR2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007878-RA">
        <property type="protein sequence ID" value="MESCA007878-PA"/>
        <property type="gene ID" value="MESCA007878"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036749_0_0_1"/>
    <dbReference type="OMA" id="ICEATEC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008472">
        <property type="entry name" value="DUF753"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721">
        <property type="entry name" value="GH09876P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05444">
        <property type="entry name" value="DUF753"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577681" description="DUF753 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="2">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="2">
        <location>
            <begin position="250"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007878-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="429" mass="47869" checksum="85CB61C3E95BE731" modified="2013-10-16" version="1">MESKSILAILFVCLSATAEGLNCIQCDSKDNKDCVANIPNLPTNSVCIQDNSLCFSKVNLTDGTLERGCLDFTELENCMTDEHCSFCNNEEQCNNGIFPRNRLECVVCEDCHENATLPQPQVCDKYFADDSCFTNTDPKTMKTSRGCGSQGVPVCGDCLYETCKGDGCNKDIPEIPNFTCYECVGSDENCIRGLEVRCTHGTQCLVKYDAESKKVERRCVASKDETCNDQFCKVCKGQDCNGGTFPEDRLSCLQCTSENNTDDCYNGTATSNTCLSYDRFDRCYSFVDHNGQIHRGCETDKYCQSSDKCYFCGTNSCNTINKPDELKPSQFECFKCLGVEAACLLGTTQKEVCQHGNSCIMKVTDDKKIERRCISNEEEKCEAGSNCVLCDGNQCNGGVFPKDKVELFYMVMIKDNKDRTRMFSEIYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWS1</accession>
    <name>T1GWS1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02157404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWS1"/>
    <dbReference type="STRING" id="36166.T1GWS1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008251-RA">
        <property type="protein sequence ID" value="MESCA008251-PA"/>
        <property type="gene ID" value="MESCA008251"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2040736_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053202">
        <property type="entry name" value="EGF_Rcpt_Signaling_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34009">
        <property type="entry name" value="PROTEIN STAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34009:SF2">
        <property type="entry name" value="PROTEIN STAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008251-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="14124" checksum="34F5CDCF81AE0405" modified="2013-10-16" version="1">MDFTYTIMHANRSQVQIINAGITNVDRPIITSLFDLYFLRKESWEYPIYTLLLGLQTMNCDLLSLGGYGNELNILENLPFHKVTIDILAIHLTRDKHPNIDKYGRLIEDFLSLQSYNFLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GX42</accession>
    <name>T1GX42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCR4-NOT transcription complex subunit 1 HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GX42"/>
    <dbReference type="STRING" id="36166.T1GX42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008382-RA">
        <property type="protein sequence ID" value="MESCA008382-PA"/>
        <property type="gene ID" value="MESCA008382"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_480294_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="2">
        <location>
            <begin position="342"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008382-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="568" mass="62745" checksum="3283487287D36E4E" modified="2013-10-16" version="1">MDEKLYWNYVNEEFGDQSNYGNILVKFIKGNGYEFTASVDECKNHLKCGGNELQPADIAKIISFMCKTHSSLNDGNANNLPTPGTVWSAKNASSNNNSESSSEKPESGTEQQQQSTENKQPEESGNTDSTVANSGSGGNNSPTWKPEVFVQAVKEILPNIVWKDVCVQLDNKEFFIRDRQGLNLLLTVFRLGTGSNLFPTELIYRHWQNVIGQLSLITIILKNPDLFSFADYIFTPAPVEVLKTPPENENKEIATWKSLHLVDALLYIADHQPQTQQCYRQVHEIFSFPVKQCPDVLFMALLQISPPLTSLRIELISFKLGNYTPTCMELYSSTSVATINYESMSDCYLRSDCDQTKLSRILDVAQDLKALSSLLNARSFMFVIDLACLASRREYLKLEKWLSDKIREHGEQFVQAIVKFLQRRCPQIMGKNDDQQLMIPKSVQLPLETVTTMVACLQSCANNVQQELCDLIMQMSNYCNILTKQARQQPPPTGVPPLTATTTTPGPAASNLNLMRQQPGPPGPGQHHQNPSGPPFNPQIFGNVGLGGAGLGGAGLGGALGGMGVNVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXH3</accession>
    <name>T1GXH3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02387453">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02387454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXH3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008524-RA">
        <property type="protein sequence ID" value="MESCA008524-PA"/>
        <property type="gene ID" value="MESCA008524"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725069_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008524-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8346" checksum="963FD57B3AE74872" modified="2013-10-16" version="1">MICIIPAGEHTEQMVDRRESLVRSILYQGCVAITTWQPNNYGKQTAFFKQLDKAYTKLPEYDMKLVLCNFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXW4</accession>
    <name>T1GXW4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02138540">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02138541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA008676-RA">
        <property type="protein sequence ID" value="MESCA008676-PA"/>
        <property type="gene ID" value="MESCA008676"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129492_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF14">
        <property type="entry name" value="OSIRIS 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008676-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="178" mass="19582" checksum="0ED0AA803A8DFDB0" modified="2013-10-16" version="1">MILRRVLSFLQTFTLQVKFPNDNDIRRSAVEEGRNHKEKKKHHKGAMIMIPLLLGGTMIPIAYGALAMLAGKALIVSKLALVLASIVGIKKLLSSGGHKESQHEVVVSGGHASGWGRDFDLALNSYAPKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSFFKIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYL7</accession>
    <name>T1GYL7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02389109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02389110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008950-RA">
        <property type="protein sequence ID" value="MESCA008950-PA"/>
        <property type="gene ID" value="MESCA008950"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2592488_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008950-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="80" mass="8883" checksum="2AC7B14F0A5AC6CF" modified="2013-10-16" version="1">MDYIFRSTVPIPEPHAVVIALTSATIMTTKGRQRKNSSSPLEGSVYYHRNRAERNEAYVKSHPGNECVLAPLEGAKPLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZ28</accession>
    <name>T1GZ28_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZ28"/>
    <dbReference type="STRING" id="36166.T1GZ28"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009119-RA">
        <property type="protein sequence ID" value="MESCA009119-PA"/>
        <property type="gene ID" value="MESCA009119"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="OMA" id="FIQHESY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577247" description="Peptidase S1 domain-containing protein" evidence="11">
        <location>
            <begin position="17"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="35"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009119-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="252" mass="26667" checksum="B890592C9D7C2893" modified="2013-10-16" version="1">MKLFLLLSALLVVASATNLRFPAAKLKDILPDSRIVGGQLANPGQFPYQVALSLSDDWSSWFCGGSILSPDWILTAAHCTYGAKSAWVYAGTLDVNTLDSSAKAIQVTSASSFIIHANYNPNTLNNDILEVGNTFEGQTVTASGWGKISDSTNSITNLLRWTDLTVENQNTCSNYYMAGLVNAGVLCTNTRGGAVSTCNGDSGGPLVLQSTGRLIGVTSFVSAAGCQSGGPDGFTRVTYYLDWIKQNTGLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1H001</accession>
    <name>T1H001_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02168526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H001"/>
    <dbReference type="STRING" id="36166.T1H001"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009463-RA">
        <property type="protein sequence ID" value="MESCA009463-PA"/>
        <property type="gene ID" value="MESCA009463"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393319_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009463-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="196" mass="22167" checksum="A088B545AC06959F" modified="2013-10-16" version="1">MLSPTKPTTMQRGSIPTHAQGARNASNISRRPVATQQRGNIVIKEETSFDPSPFEQLRQSGINYKRPASKTYETPPEKKPSIEDVKEFAEQQRMRKQIAAEVSGIPAEDEYVESYMEDEFTQDDEDEDNTGAQITIKQEPNQKPTIVFKASADNKMDHAKIIGEVLKKYPHLIKNNKNLKLKIMPNSGPSQQQKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0G3</accession>
    <name>T1H0G3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02092995">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0G3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009641-RA">
        <property type="protein sequence ID" value="MESCA009641-PA"/>
        <property type="gene ID" value="MESCA009641"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2266806_0_0_1"/>
    <dbReference type="OMA" id="CSFAHPL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588633" evidence="1">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009641-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="10688" checksum="24148925895ECB2B" modified="2013-10-16" version="1">MKLILLIFFAIFVYICDAVPVSTEHQINSLLDVDTENGHSNEGNRKARFLGGWGWSGTGWGGWGGGWGGHNHGHGCCGCGGWGGWGGWGGGCGGCGGWGCGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2A0</accession>
    <name>T1H2A0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2A0"/>
    <dbReference type="STRING" id="36166.T1H2A0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010337-RA">
        <property type="protein sequence ID" value="MESCA010337-PA"/>
        <property type="gene ID" value="MESCA010337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075666_2_0_1"/>
    <dbReference type="OMA" id="SCQASKM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000043">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="2">
        <location>
            <begin position="35"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="141" mass="16447" checksum="EB2D6742EAA997C8" modified="2013-10-16" version="1">MLNLDILNEETYTKNDKTYKKLGFTEAYKEKVIKQRRTKAKKLRRSTRPIFKSYIHKVLRIVHPDLSISKKGINIMDSLVCDVFHRFSCQASKMSKISKRRTLTVQDFQSSTRLILPGDLGQHALLEGRKAVNNFMKSRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2G9</accession>
    <name>T1H2G9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translocon-associated protein subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Signal sequence receptor subunit delta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAP-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02377193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2G9"/>
    <dbReference type="STRING" id="36166.T1H2G9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010414-RA">
        <property type="protein sequence ID" value="MESCA010414-PA"/>
        <property type="gene ID" value="MESCA010414"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100264_0_1_1"/>
    <dbReference type="OMA" id="QCSNNPK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008855">
        <property type="entry name" value="TRAP-delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12731:SF1">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12731">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, DELTA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05404">
        <property type="entry name" value="TRAP-delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577327" description="Translocon-associated protein subunit delta" evidence="16">
        <location>
            <begin position="20"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010414-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="159" mass="16932" checksum="7470A1709E09EB07" modified="2013-10-16" version="1">MFKFVVFAVLSVFAASAYGACTSPKASATSFTSSDATIVSQVATVVDLSFKCSNGETPQLYGEINGRVILASKISWNDEVKKAISGSVTIRLFDEEGYSNIRKAQREGVSLSSVKSISEVTVSVPSAYTGPLVNSELLVAFLAIFIGYFAFTTKSKLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-09-13" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2K7</accession>
    <name>T1H2K7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IGFBP N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02124761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2K7"/>
    <dbReference type="STRING" id="36166.T1H2K7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010456-RA">
        <property type="protein sequence ID" value="MESCA010456-PA"/>
        <property type="gene ID" value="MESCA010456"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764114_0_0_1"/>
    <dbReference type="OMA" id="NDCPRTE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010456-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="36595" checksum="B3BA21D6DD97CE88" modified="2013-10-16" version="1">MCEPCNIPCCAPCDPCSGAYEPSPQCYSGAVLEQLPQCAPRPPPPRPKCITIQQPPLNRCRQITIPKVVDTTRCIKVPKLTWVSQLIREPRVIYYPSMIPDPYVVSYPKTVCEPREVCQAFLCQPKPQVIDIPPPSEYCCYPNGPLKYKPTMPCPPSPLSPCSGCAPCGPQGPCGPIPTYGYSSFCGPCSPCGPCGPCGPCGPCGPCGPCGPCGPCGPCGPCGPGACGPCGPYGPLPCGLCGPCGPCSPCGPCGDSCGPCGPCGPCEPGGPCGPCGPCGPCGPCGPRGLVVPSGPCVPYCSPCDPMKNYVCGPCGPCEPCNPCGNIGGCGPCGPCGYGPCGPPACDPCSPSTPSSPFMGCPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2X4</accession>
    <name>T1H2X4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02386560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2X4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010580-RA">
        <property type="protein sequence ID" value="MESCA010580-PA"/>
        <property type="gene ID" value="MESCA010580"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1867431_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010580-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="137" mass="16254" checksum="746FE5F124373392" modified="2013-10-16" version="1">MSSSLDCRSVYSLEMKLFQAIFLTFCINIVRANYVQEKCEKEAEELKNTPLHNKFLKDCVSTGTWTVTEDEYFKKWEECKEKTGYTSHQECLSMNNIHNEDMMAHYDCFDEIIDPDHHNDNNVYRFTVIDLIHYNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Y2</accession>
    <name>T1H2Y2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Y2"/>
    <dbReference type="STRING" id="36166.T1H2Y2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010588-RA">
        <property type="protein sequence ID" value="MESCA010588-PA"/>
        <property type="gene ID" value="MESCA010588"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461101_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010588-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="89" mass="10004" checksum="EF7A6BB2A08559A6" modified="2013-10-16" version="1">MSVITSLFGHLAIHCKKTTPICVNCAGDHCKDDCQATFKNCANCGNAHPATDKSFPKREEYSRIHKIMILDNLNYREAAKKLSVPDFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H356</accession>
    <name>T1H356_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Apolipophorin-III</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02388023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H356"/>
    <dbReference type="STRING" id="36166.T1H356"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010671-RA">
        <property type="protein sequence ID" value="MESCA010671-PA"/>
        <property type="gene ID" value="MESCA010671"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1456022_0_0_1"/>
    <dbReference type="OMA" id="LESVWTN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010009">
        <property type="entry name" value="ApoLp-III"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07464">
        <property type="entry name" value="ApoLp-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47857">
        <property type="entry name" value="Apolipophorin-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577339" description="Apolipophorin-III" evidence="1">
        <location>
            <begin position="19"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010671-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20579" checksum="5BCDA45C47517BF2" modified="2013-10-16" version="1">MSKLFFVISALLLATASARVVRDAPAGNEIETVFKNFEQLFNEGKKNVFDFMGVKNDEEAQTLVKNEAQNFFDKLAGIGKELKKSSEDVAAKIENDETVKSFRAKVTEAIEDFKKANPEVTEGATKLNEKLQGTLETIAAEAKKLGESAQNPELQQQFKDIAKKVLDTTTDTFKDLNTKLAQVGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H385</accession>
    <name>T1H385_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02374363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H385"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010704-RA">
        <property type="protein sequence ID" value="MESCA010704-PA"/>
        <property type="gene ID" value="MESCA010704"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190180_1_0_1"/>
    <dbReference type="OMA" id="SRRICDC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054721">
        <property type="entry name" value="GEO12453p1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22861">
        <property type="entry name" value="GEO12453p1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588701" evidence="1">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010704-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="73" mass="7976" checksum="ACCFAE18C3867F98" modified="2013-10-16" version="1">MFKFVSIVVMALIAFVAAKPQIVAPLAYSSAYTYPAYSAYSAGYAYPYAAAYSSPYTAAYSTYPYASAYTLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H399</accession>
    <name>T1H399_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H399"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010719-RA">
        <property type="protein sequence ID" value="MESCA010719-PA"/>
        <property type="gene ID" value="MESCA010719"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2657304_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010719-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8876" checksum="8E094D01E109017D" modified="2013-10-16" version="1">MQKDLLKSTRAGNRRILENRNLYGILRKPWVKEVGKNKIGTLAVTASLLKKDQRSQTKRTKKSGRTFRNHKEIQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3B5</accession>
    <name>T1H3B5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02380141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02380142">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3B5"/>
    <dbReference type="STRING" id="36166.T1H3B5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010735-RA">
        <property type="protein sequence ID" value="MESCA010735-PA"/>
        <property type="gene ID" value="MESCA010735"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2087552_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF76">
        <property type="entry name" value="ADULT-SPECIFIC CUTICULAR PROTEIN ACP-20-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010735-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="117" mass="13303" checksum="85D08AF6EF23B359" modified="2013-10-16" version="1">MDLILSNVFQSVGSSEAKGQNSLRIVFEIGDKQRFHYFASFEIIKLRIDLYEYNFEVKDAEKDLFFNKNEAGDQSGKVSGQYSVWLPDGRLMTVVYTVDSESGFVPKITFDTNNPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3J9</accession>
    <name>T1H3J9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="9">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="9"/>
    <dbReference type="EMBL" id="CAQQ02373999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3J9"/>
    <dbReference type="STRING" id="36166.T1H3J9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010827-RA">
        <property type="protein sequence ID" value="MESCA010827-PA"/>
        <property type="gene ID" value="MESCA010827"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_1_0_1"/>
    <dbReference type="OMA" id="HAWDCLA"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000004">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000002">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Inositol_monoPase_Li-sen"/>
    </dbReference>
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        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00378">
        <property type="entry name" value="LIIMPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010827-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="276" mass="30359" checksum="452B755921E9F841" modified="2013-10-16" version="1">MSSDDRNLDKCYEEVLKLVDQAGELISKRNDSRKEFVCKSGDIDLVTETDQEVEKLLVKGLSSRFPSHMFIGEEDSADGVKVKLTNNPTWIIDPVDGTMNFVHGFPHSCVSIALLVDKVPEIGIVYNPMFNQRFTARRGQGSYYNGKKLDGQKDLSKALIISEYGTSRDTEKLQAVVANFKVMAEKAHGIRVLGSAALNMCHVAMGCADANYEMGLHAWDMAAGDLIVREAGGVVIDPSGGPLDIMSRRVLAASSTELAQEIVKHLTQYHPLPRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3K9</accession>
    <name>T1H3K9_MEGSC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="36166.T1H3K9"/>
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        <property type="protein sequence ID" value="MESCA010837-PA"/>
        <property type="gene ID" value="MESCA010837"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010837-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14118" checksum="098ED1E69402AB9D" modified="2013-10-16" version="1">MAMLSRVASNYIGAELLVEDNLLGVLSYMKVYDLHRDFQINYQQLQAMEDDSFVPAVDKRFQQILFPALNICDVILSTLGIGNRSEISQIIHFLVSHGDVIEIVLRAGTPFMNLGMLQDLSSGLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3U9</accession>
    <name>T1H3U9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CARMIL pleckstrin homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3U9"/>
    <dbReference type="STRING" id="36166.T1H3U9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010941-RA">
        <property type="protein sequence ID" value="MESCA010941-PA"/>
        <property type="gene ID" value="MESCA010941"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2335989_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041245">
        <property type="entry name" value="CARMIL_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17888">
        <property type="entry name" value="Carm_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARMIL pleckstrin homology" evidence="1">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010941-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="11162" checksum="F3E741719DF49A7F" modified="2013-10-16" version="1">MNKFEKVPHFCRNSWEFCGGIEHHFHYLDIQAVESKKPVHFSIITHDRTYSFLTTGDAGNFSSNADVILTDLASAIKQIFPTVPLKYIIRKVSTAGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H445</accession>
    <name>T1H445_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02376914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H445"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011046-RA">
        <property type="protein sequence ID" value="MESCA011046-PA"/>
        <property type="gene ID" value="MESCA011046"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2298722_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011046-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11710" checksum="8FCFA1D03DB6C9A5" modified="2013-10-16" version="1">PCHLHKHTETDRCFRCRPLNNPLVLITPALSASVYNFIMYPLTLQYTKPSSIAPARYLKIMFTATVYCTLFRVLYRSIRRSRLVLEKLVSVNPIVPKIIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4K9</accession>
    <name>T1H4K9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4K9"/>
    <dbReference type="STRING" id="36166.T1H4K9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011221-RA">
        <property type="protein sequence ID" value="MESCA011221-PA"/>
        <property type="gene ID" value="MESCA011221"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3160507_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF227">
        <property type="entry name" value="SYNAPTOTAGMIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011221-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="48" mass="5631" checksum="CFB40E57362F3DCE" modified="2013-10-16" version="1">MGGTSDPYVKVYLLPDKKKKFETKVHRKTLSPVFNETFTFKVVYMEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1H565</accession>
    <name>T1H565_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02130418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02130419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H565"/>
    <dbReference type="STRING" id="36166.T1H565"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011440-RA">
        <property type="protein sequence ID" value="MESCA011440-PA"/>
        <property type="gene ID" value="MESCA011440"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1158882_0_0_1"/>
    <dbReference type="OMA" id="WMQANDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF127">
        <property type="entry name" value="LD30826P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="179"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011440-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="27767" checksum="6F66BBD03A1DBBFA" modified="2013-10-16" version="1">TGCFNNLSAEDLKVAFRLYRGPLLIIEFIFLIGVNIYGWRSSGVNHVLIFELDPRNHLSEQHLMELAAIFGVIWTLSILSFLYSTALSIPAYANPLTLTLIMLVFLLNPFHVLHHDARFWLLRISFRMFSAPFFHVGFADFWLGDQLNSLVTALMDFEFLTCFYITNGNDWMQANDSSMCMEKDFYVRPIVNCIPAWIRFAQCLRRYRDSKEAFPHLVNAGKYSTTFMVVIFGTLRSFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5C9</accession>
    <name>T1H5C9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine/threonine-protein kinase greatwall</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Microtubule-associated serine/threonine-protein kinase-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAQQ02064150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5C9"/>
    <dbReference type="STRING" id="36166.T1H5C9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011506-RA">
        <property type="protein sequence ID" value="MESCA011506-PA"/>
        <property type="gene ID" value="MESCA011506"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998251_0_0_1"/>
    <dbReference type="OMA" id="IMSERNM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000550">
        <property type="entry name" value="Serine/threonine-protein kinase greatwall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="43"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011506-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14481" checksum="EA71903CFEACD065" modified="2013-10-16" version="1">MEPKTPKRVQILDGEHMLDKINKLTTKRDNKESPTKLPSIRDFVIIKPISRGAYGKVFLGYKQNDSDKLFAIKVMRKSDMINKNMVSQVITERNALALSRSPFCVNLYYSLQSVSYIYLVLEYMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5D7</accession>
    <name>T1H5D7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H5D7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011514-RA">
        <property type="protein sequence ID" value="MESCA011514-PA"/>
        <property type="gene ID" value="MESCA011514"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357665_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011514-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="202" mass="23447" checksum="0FD8682F71DC1DC3" modified="2013-10-16" version="1">MSFFVPSKEIETCCHTKRLGAKRTQVLASVYDDFHDVLSPRERAKRINEELEGFRSITTEQLESELRDFAAREEKKMKIAKNKAHKRAQLLKKCERACSEKILFKLVKRFASYVADRCENFEPPAVETESFQKMQQMLMCNLYLNLGDNKKISPDPNIAKMITVVIADLIMQIMFLAQNAHKYEEKQDICEPFLFEPQSSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5U2</accession>
    <name>T1H5U2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ecdysteroid-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-deoxyecdysone 22-phosphate + H2O = 2-deoxyecdysone + phosphate</text>
            <dbReference type="Rhea" id="RHEA:63584"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:19566"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:147386"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>20-hydroxyecdysone 22-phosphate + H2O = 20-hydroxyecdysone + phosphate</text>
            <dbReference type="Rhea" id="RHEA:63580"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16587"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:147382"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ecdysone 22-phosphate + H2O = ecdysone + phosphate</text>
            <dbReference type="Rhea" id="RHEA:63576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16688"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:147380"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H5U2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012075-RA">
        <property type="protein sequence ID" value="MESCA012075-PA"/>
        <property type="gene ID" value="MESCA012075"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_456802_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102531">
        <property type="term" value="F:ecdysteroid-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14301">
        <property type="entry name" value="UBA_UBS3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000053">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing, A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="UBA" evidence="11">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="266"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012075-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="598" mass="67130" checksum="F15C381C2C27D90D" modified="2013-10-16" version="1">MATLPPRKNLTPTRSTKQSLTPLQVLMQMGFTKARAEKALASTGYRGVQLASDWLLAHVNDPHLDEFSFSRREYILYACPTGPFQQQLEDFWLKSKEQCGWNGAHNYFPHITLVSFFKAEDENALNLSKSLKQVVDMCGAVLERPLKLELYTSPNFLGFFVAEDDANFLKRLALSYMKEVSNSIISDNYEQLDALVACFPWCGAVTSSGNRCIPRSSRSISLEPHVKSLHLTLAYQFPQSQFNILKNLAENLDPTCASSWELRLYSRDTRLATKQAHAFEVDELELRIGDFVYLNGDLVDSSSDGWTEGISWLTGQTGFLPVNYTERTAESDAWTLHKVVPLSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5V5</accession>
    <name>T1H5V5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Malate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H5V5"/>
    <dbReference type="STRING" id="36166.T1H5V5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012088-RA">
        <property type="protein sequence ID" value="MESCA012088-PA"/>
        <property type="gene ID" value="MESCA012088"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040452_5_1_1"/>
    <dbReference type="OMA" id="GEVGDQY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="entry name" value="Hypothetical oxidoreductase yiak, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091:SF0">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="L-sulfolactate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012088-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25348" checksum="598A1C06EE8E1CB6" modified="2013-10-16" version="1">SNHYGMAGWYAIRAMKEGLIGISMTNTTPLMAPTRSKEAALGTNPIALGASAKNGDKFLLDMATTAVSLGKVEIQIRKDEPIPDGWAQDSSGNVTNFKKHSKKVVMPLGGFEITSGYKGYGLGAMVEILCGVMAGSNYSTHVRDLTEEDCEANLGQVFIAVDPNQFANGFEDRLTDMNDILRSCKPTDPKNPVLVPGDKEKHNMEDVNKAGGIQYLPNQLQTCENLAKRLNIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1H5X2</accession>
    <name>T1H5X2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TSC22 domain family protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H5X2"/>
    <dbReference type="STRING" id="36166.T1H5X2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012105-RA">
        <property type="protein sequence ID" value="MESCA012105-PA"/>
        <property type="gene ID" value="MESCA012105"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2055589_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21936">
        <property type="entry name" value="ZIP_TSC22D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000580">
        <property type="entry name" value="TSC22/Bun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745:SF1">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01166">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58026">
        <property type="entry name" value="Delta-sleep-inducing peptide immunoreactive peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012105-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="12605" checksum="54BF26FCBC8F6139" modified="2013-10-16" version="1">DLVKSHLMIAVREEVEVLKEKITELMEKINLLEVENTILKKNVPQETLQANNVATGGAAAAQQQPAAATNPTATTISNHTTDNVVTANTNATTTTKCRAATVVAGATPATNHTTNSNQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1H691</accession>
    <name>T1H691_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H691"/>
    <dbReference type="STRING" id="36166.T1H691"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012224-RA">
        <property type="protein sequence ID" value="MESCA012224-PA"/>
        <property type="gene ID" value="MESCA012224"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061042_1_0_1"/>
    <dbReference type="OMA" id="FPTCEIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044716">
        <property type="term" value="F:8-oxo-GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04671">
        <property type="entry name" value="NUDIX_8DGDPP_Nudt18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042970">
        <property type="entry name" value="NUDT18_NUDIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22769:SF56">
        <property type="entry name" value="8-OXO-DGDP PHOSPHATASE NUDT18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22769">
        <property type="entry name" value="MUTT/NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="53"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012224-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="326" mass="36385" checksum="38341232E8F601B4" modified="2013-10-16" version="1">IEDKLNRVFRIQDLDDITSEVCDFSLEEQNAATEAQGIQPSTSSDYVPVIGKSVTYVVVCVLINDQNEVLMMQESKPSCAGKWYLPAGRMESGEHICEAAAREVLEETGIETEMTTLLGIEAAGGSWFRFILTGKVLGGTLKTPDQADRESMQAKWIGDLNELTLRANDIINVIEIARSYKNKGENPWHADILPIRFSHHKSFLRVVAVARKRTSNSLNMLVSEKNVHHLPTVEIHPGRSVYSTLRKFMVELFGAELPQHRPHGVLTLEHNPSAPPHTTDGICVTLLVAFRPPVEECNIIGKCIWHELKKALESKLVNILSNKNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6H0</accession>
    <name>T1H6H0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6H0"/>
    <dbReference type="STRING" id="36166.T1H6H0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012303-RA">
        <property type="protein sequence ID" value="MESCA012303-PA"/>
        <property type="gene ID" value="MESCA012303"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830201_0_0_1"/>
    <dbReference type="OMA" id="CEDAYER"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31511">
        <property type="entry name" value="PROTEIN CBG23764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31511:SF12">
        <property type="entry name" value="RHO TERMINATION FACTOR N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012303-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16633" checksum="87F2AFCD6DF9823D" modified="2013-10-16" version="1">DPHSHRTFSDNFAAMELKKKNIIYDKPIYVGFAILELSKEIMYSFYYDYMKPKFVNNVEICYQDTDSFILAIHDKDFHEKIKADIPERFDTSNLKPENNKFGFPILNHRVLGMMKDETGWIPIHKFVGLKSKMYAIKIADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6M9</accession>
    <name>T1H6M9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6M9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012362-RA">
        <property type="protein sequence ID" value="MESCA012362-PA"/>
        <property type="gene ID" value="MESCA012362"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2203575_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012362-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12621" checksum="3F8D6C817EEDDB43" modified="2013-10-16" version="1">MLLVFHDKHTKWSEFSPLRAATSVSLINSFRERIYHGLLSKNLITDNGPYNITPPYTPQCNPTERVNRVIKTMMAQYXNKEWDEYLPELQMAYNTSIHEATQFSPAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6R3</accession>
    <name>T1H6R3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coronin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="STRING" id="36166.T1H6R3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012396-RA">
        <property type="protein sequence ID" value="MESCA012396-PA"/>
        <property type="gene ID" value="MESCA012396"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026859_0_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF0">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 2" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012396-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="326" mass="35947" checksum="CF51D7E552C9C36B" modified="2013-10-16" version="1">VESAGGGAFIVLPHNKAADHPLVGGHKGPVLDIAWCPHNDNVIASGSEDCVVKVWQIPDGGLSRTLTDPVVDLVFHQRXXXXXXXXXXXXVVIWNVGTGEILVHIDSHPDIVYSACFNWDGSKLVTTCKDKKIRVYDPRSGEVESEALCHEGSKATRAIFLRHGLIFTTGFNRSSERQYSLRAPDALSEPIVMVELDTSNGVMFPLYDPDTNLVYLCGKGDSVIRYFEVTPEPPFVHYINTFQTSDPQRGIGIMPKRGCDVTTCEIAKFYRLNNSGLCQVISMTVPRKSDLFQEDLYPDTISDEAAQTAEDWIGGQDAEPILCSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Y8</accession>
    <name>T1H6Y8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="36166.T1H6Y8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012470-RA">
        <property type="protein sequence ID" value="MESCA012470-PA"/>
        <property type="gene ID" value="MESCA012470"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099287_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF1">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012470-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14591" checksum="98B39EB318F6E16D" modified="2013-10-16" version="1">MFDLNGDGEGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGVNSALTTYFFGRKMDQKLTIEKFLDFQQQLQREILSLEFLRKNPDENGNISEVDFAELLLAYAGLNEKKKQKKLKRVKRRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7C1</accession>
    <name>T1H7C1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ribosomal subunit protein mS40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S18-2, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">28S ribosomal protein S18b, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS18 family. Mitochondrion-specific ribosomal protein mS40 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7C1"/>
    <dbReference type="STRING" id="36166.T1H7C1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012659-RA">
        <property type="protein sequence ID" value="MESCA012659-PA"/>
        <property type="gene ID" value="MESCA012659"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270477_0_0_1"/>
    <dbReference type="OMA" id="REYEYSD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.10:FF:000008">
        <property type="entry name" value="28S ribosomal protein S18b, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040054">
        <property type="entry name" value="MRPS18B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13329">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S18B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13329:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="103" mass="11901" checksum="8E2945192B21CD4C" modified="2013-10-16" version="1">RNHKGLYPRKTRKTCVRKGFLATGNPCPACRDEYLILHPKNVDLLKQFISPQTGQVLSYSKTGLCQMKHLELLVAVEQAMDAGLITFDVPFRKYDYSEYYDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA83</accession>
    <name>T1GA83_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02152645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000133-RA">
        <property type="protein sequence ID" value="MESCA000133-PA"/>
        <property type="gene ID" value="MESCA000133"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3406725_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000133-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="30" mass="3604" checksum="7C21A8B1E0D4E55C" modified="2013-10-16" version="1">MWSDMIFGLLVNSNSLQRLRFLSRIPFQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAJ7</accession>
    <name>T1GAJ7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02390924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAJ7"/>
    <dbReference type="STRING" id="36166.T1GAJ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000255-RA">
        <property type="protein sequence ID" value="MESCA000255-PA"/>
        <property type="gene ID" value="MESCA000255"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_829737_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="35"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000255-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="335" mass="37797" checksum="F7A6939B53961DED" modified="2013-10-16" version="1">MPMTVLTYEAYKPENQTLMEELIRQFNDIKFYKSYLVSVRVRVDQPLGMKTYEHLCGGTILNRHKILTAAHCFYFTNVAGNSTRIPQEMIYIMQDSENLFEYENAFVVEATRVDIHPKYVTRDFTNDLAIVTTSKAFFIGDTVHLPSRSTAIRDIGIISGYGRLYQRGPFSGFALHAFVVIGDCIEMNISYTNQPTFCAMDYEHEGEGVCEGGFGGGLLSPHFEVIGVLSKGSVCASQGQPGIYTEVYSHKMWIENVLWDFVVTLQLDIPSSLASLHNASYRYAYDKFLNINKSSDGCSSCTKRSTSRHGLHLIASNKMKYARAKYRSMEDNSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GAV5</accession>
    <name>T1GAV5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02116609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02116610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GAV5"/>
    <dbReference type="STRING" id="36166.T1GAV5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000370-RA">
        <property type="protein sequence ID" value="MESCA000370-PA"/>
        <property type="gene ID" value="MESCA000370"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_987942_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000370-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="282" mass="32177" checksum="42F2356D95158BE3" modified="2013-10-16" version="1">MGGGLSQVFRSGSALNKNSSKATDDYFEAIFEILRANPKTPLLKLEFALQQIPKHENILLFHDEYGYNILQKAVGLNHIELTKWLLHRHRPDVNRSLCSLPLHIACLKGYEECVDLLLKYGARIDTEARMCFSGVHSPNCEESGKYRISHEGHCSYTNKLQNAICYAIDGDQINVLNMLIQKMEEPWVPFRAKKPLLHLACERGAFRCVQQLVITRSEEINLIKDEFYPIHHAVLKDVRFIELLINNGANVQNASLWRIFEFADFPGFSPVAPLKSKLLSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB08</accession>
    <name>T1GB08_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L18a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02391152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB08"/>
    <dbReference type="STRING" id="36166.T1GB08"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000428-RA">
        <property type="protein sequence ID" value="MESCA000428-PA"/>
        <property type="gene ID" value="MESCA000428"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080773_2_0_1"/>
    <dbReference type="OMA" id="CIFAKND"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="5">
        <location>
            <begin position="7"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000428-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="177" mass="21012" checksum="AEB7E78AF7144E23" modified="2013-10-16" version="1">MKAKGQLKEYEVIGRKLPTEKEPQTPLYKMRIFAPDRIVAKSRFWYFLRQLKKFKKTTGEIVNVKQVYETSPTKIKNFGIWLRYDSRSGTHNMYREYRDLTVGGAVTACYRDMGARHRARAHSIQIIKVEPVVASKTRRPHVKQFHNSKIRFPLAQRVHHKGSRKLFSFRKPRTFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB32</accession>
    <name>T1GB32_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02004577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02004586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB32"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000453-RA">
        <property type="protein sequence ID" value="MESCA000453-PA"/>
        <property type="gene ID" value="MESCA000453"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929421_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577225" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="61" mass="6655" checksum="26917C29A9CF583C" modified="2013-10-16" version="1">MSPFYFISVILFALTALQPRANAQSLFDDGLSTQTIYGFLDFTTTIGNTVMVFLPSSVPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBD1</accession>
    <name>T1GBD1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBD1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000563-RA">
        <property type="protein sequence ID" value="MESCA000563-PA"/>
        <property type="gene ID" value="MESCA000563"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2998826_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577239" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="57" mass="6409" checksum="14A5A4C88B529202" modified="2013-10-16" version="1">MTRNFVLIALVAIGFVAITAQAEHAQSFEKFMLDLMSPQILDTEYANVEKKVLYSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC17</accession>
    <name>T1GC17_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02062810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02062811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC17"/>
    <dbReference type="STRING" id="36166.T1GC17"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000812-RA">
        <property type="protein sequence ID" value="MESCA000812-PA"/>
        <property type="gene ID" value="MESCA000812"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030838_1_0_1"/>
    <dbReference type="OMA" id="KFKYERR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000148">
        <property type="entry name" value="zinc finger protein Gfi-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000245">
        <property type="entry name" value="zinc finger protein Gfi-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000208">
        <property type="entry name" value="zinc finger protein Gfi-1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000432">
        <property type="entry name" value="zinc finger protein Gfi-1b isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="91"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000812-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="443" mass="50348" checksum="837CA59F17CFD430" modified="2013-10-16" version="1">MEHLSPPPSPQSSSASSTAAAIINHNNNNLFEASSSSSKNWIQRASAFNSVLAASAGTKLNRDLPFFYNPLLYSSALLWPQFFFPSAAQTPNSGLATPSTPATPSSPYFEQEPEKLTPTNQSDLSLTPEKDDSLQQEDDMPLNLSVKRSRSKKSDMVTSGYVRERNIFDPIAKKFKYERRSNYPRIIKRDEDYDDMQMTPTGIPKTDGSPTKFSQQLFITQQNLYKQHQDILQQHFKNAAAVATATANKREMDILRQNRTDIYLMNNNNNANSVNQNQISVLSGNKFNFDSGEKIVTTPADSTGLQNWREESLEKRNKPEKSFECKQCGKTFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTYIHTGEKPHKCTVCLKAFSQSSNLITHMRKHTGYKPFACGMCDQSFQRKVDLRRHSESRHEDTFTASPVSKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCQ7</accession>
    <name>T1GCQ7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02016105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCQ7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001077-RA">
        <property type="protein sequence ID" value="MESCA001077-PA"/>
        <property type="gene ID" value="MESCA001077"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387333_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001077-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="22632" checksum="8A49DB2272254C1B" modified="2013-10-16" version="1">SIEIESISELNEDDFAIAVNICKEEIYILNGHNKVYVNKSLILNTSVVEILEGDLIGIFSNKCDENQNYEAEFRLLRASKSANNSITEEVQIAPVIEILDSDDEDSIEITERIVANIDLSDEFSEDEYIAKKMYGEDLPNDDEIICVDEQNLIEKWRREKFKPIVKQEPIDGELDLKQIKSEMESLPNDEETKIRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCZ5</accession>
    <name>T1GCZ5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02055437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCZ5"/>
    <dbReference type="STRING" id="36166.T1GCZ5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001170-RA">
        <property type="protein sequence ID" value="MESCA001170-PA"/>
        <property type="gene ID" value="MESCA001170"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2564993_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001170-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="8976" checksum="A8BFC006DCE5AB5A" modified="2013-10-16" version="1">MDSHNLILSDDISSLDSSNSQNSNKYYQIDPFINYREESNDTSSSQHLLSPSTLSKSPDAASSLPSLCSPNSLSQDELAFMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GD83</accession>
    <name>T1GD83_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DZF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184971">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02184972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GD83"/>
    <dbReference type="STRING" id="36166.T1GD83"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001264-RA">
        <property type="protein sequence ID" value="MESCA001264-PA"/>
        <property type="gene ID" value="MESCA001264"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012026_1_0_1"/>
    <dbReference type="OMA" id="NEDEPMY"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000210">
        <property type="entry name" value="Zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002995">
        <property type="entry name" value="Zinc-finger protein at 72d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006561">
        <property type="entry name" value="DZF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762:SF3">
        <property type="entry name" value="ZINC-FINGER PROTEIN AT 72D, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DZF" evidence="5">
        <location>
            <begin position="432"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="477"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="608" mass="66733" checksum="782D17D2CB92BB4F" modified="2013-10-16" version="1">MAANNYCGFAYGGAQYSAAATPTAAAYSAAPTAYVQGAPQTYNTQRTGYEQAYSAATASAGTYGDPYSAPPVRNVIADPVASSTIKDKNTAPKQTAQAATTAPAYSTYDAALYSAASMYVAQQQQAQQKTSTWQQFSKKGQKGPRPKTVPRPQQLHYCEVCKISCAGPQTYREHLDGQKHKKREASLKLSSAATTTAQNRGNNYHCELCDVTCTGHDAYAAHVRGSKHQKVVKLHQKLGKPIPADDPKKMGKINFVPEGGAQATGQASSVNGEGSAGENVEEDHPMDDIKPVGVEYIEEQKDEDGKILSFNCKLCECKFNDPNAKEMHMKGRRHRLQYKRKVQPDLVVDFKPTPRQRRLAEARAQRALMQAEFYNRRSNIGMGNEDEPMYWDERQRYDDEMNYAGGWGGPQRGGIGRSMFGSGRNGPMGGFPPFFGSNINANMMQMRRPKVVMIVMPLLSIIVSHTERSLKLVSDAFTESKDGNNQNSGANNESNNKEDGRDNQMFSFQKDAESNKVRMLKGVMRVGLLAKGLLLQGDTLVQLVVLCSEKPTKTLLKRVATELPIKMKEVSNDTVYDVSVSDEEGSILVTDGTITVKVSLTSPLLREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDD9</accession>
    <name>T1GDD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-serine deaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">L-threonine dehydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02072204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDD9"/>
    <dbReference type="STRING" id="36166.T1GDD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001324-RA">
        <property type="protein sequence ID" value="MESCA001324-PA"/>
        <property type="gene ID" value="MESCA001324"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_1"/>
    <dbReference type="OMA" id="QTQHLGQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF19">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="367"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="453" mass="49727" checksum="51E08655C0F669D0" modified="2013-10-16" version="1">MPGSNKTNKLKPEDRSGSNDSISVKMEVLKLETNNNDVPEEEFIDPYCVEGKPQKITFQDVTSAAFLIKGGVERTPCFKSKLTDHRDLDMDLYLKMDFMQHTGSFKERGARYALLSLSEEQKKKGVISASLGNHAQALKAAPIMKIQKCRNYKANVVVFGKDMGEAKKLAMKMANDQKLLYINGYDHPHIMAGQGTIGLEILEQVEQPDAVIIPVGGGGLIAGIAVAIKSLSPKTKIIGVESEKCPSFSNAMTHKGPVHTAIKNTLADGLAVPKVGVNAYETALPLIDKMVVVKEEWIALSILQLVETEKCVVEGAGAAGLAAILAGHLPELTEKKVVLLLCGGNIDTTVFGRCLERGLAAEGRLVKFTVNVSDRPGGIHELCRLMSDLGVSIKDIMHERAWLKDIYSVEVKVVAETRDWDHSLALKKMLNEHYETVTLLKFLWRFIINLHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFA7</accession>
    <name>T1GFA7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02101122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02101124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFA7"/>
    <dbReference type="STRING" id="36166.T1GFA7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002044-RA">
        <property type="protein sequence ID" value="MESCA002044-PA"/>
        <property type="gene ID" value="MESCA002044"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2052286_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF253">
        <property type="entry name" value="DPR-INTERACTING PROTEIN ETA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="22"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002044-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13408" checksum="38E126BBF0F386A9" modified="2013-10-16" version="1">MTNIIELSPQKKKPNPKTGVPPDILDYPTSTDMVIGEGSNTTLKCAATGSPEPVITWRRENGEPIPITSGQDDSISFWDFVFNALETCFVNNLYLNINVIFFKYKFYVYVAGYGSPYGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFE9</accession>
    <name>T1GFE9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02392307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002090-RA">
        <property type="protein sequence ID" value="MESCA002090-PA"/>
        <property type="gene ID" value="MESCA002090"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2576580_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002090-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9274" checksum="7C08D1CE99CD563F" modified="2013-10-16" version="1">MKDDLPIRSCLVLSHLIHVGNPQIPLIFVYYVYPSGIKVSSIFVTLLSKHKLFFGKLFWNPNIYIVILVPESPQLSSNNYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFR1</accession>
    <name>T1GFR1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">cGMP-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02034014">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFR1"/>
    <dbReference type="STRING" id="36166.T1GFR1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002208-RA">
        <property type="protein sequence ID" value="MESCA002208-PA"/>
        <property type="gene ID" value="MESCA002208"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_73_2_1"/>
    <dbReference type="OMA" id="AFATKSH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000064">
        <property type="entry name" value="cGMP-dependent protein kinase, isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002374">
        <property type="entry name" value="cGMP_dep_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF147">
        <property type="entry name" value="CGMP-DEPENDENT SERINE_THREONIN PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00104">
        <property type="entry name" value="CGMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="172"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002208-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34629" checksum="C45679442854A5EF" modified="2013-10-16" version="1">MIMMKTGMQRIKENVNFLKSVPLLKNLSDDVLAKISDVLEVEFYTAGTYIIRQGTSGDSFFIICQGSVKVTQKINDEEKEIRVLGRGDYFGEQALINEDKRTANIIALNPGVECLTLDRDSFTHLIGDLCELKEKNYGDENRVLSIKYSEKVNDSLDQNLQQEFSDLKLSDLEVVATLGIGGFGRVELVKTFYKNKTETFALKCLKKKHIIDTRQEEHVFSERTIMLTCKSPFICRCYRTFRDKKYVYLLLESCIGGEVWTILRDKGKFQENMAQFIIGCVLEAFEYLHSRGIFIVILSLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFT5</accession>
    <name>T1GFT5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02126365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFT5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002235-RA">
        <property type="protein sequence ID" value="MESCA002235-PA"/>
        <property type="gene ID" value="MESCA002235"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2323048_0_0_1"/>
    <dbReference type="OMA" id="PKECGPD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004576886" evidence="2">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="99" mass="10099" checksum="B175D6EE759C5395" modified="2013-10-16" version="1">MGKVLLILTIALFVGLVVCKPKPPCPGPPKPPKPPGCDDKPGPPKPPGPGPKPPGPGPKPPKECGPDPPHPPPPPPPKPSTTEAPETTTDNPETTTIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGQ2</accession>
    <name>T1GGQ2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGQ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002576-RA">
        <property type="protein sequence ID" value="MESCA002576-PA"/>
        <property type="gene ID" value="MESCA002576"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294849_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002576-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11426" checksum="C1361B23D916FA31" modified="2013-10-16" version="1">MKVNIECLHSVCKADARGSLFNKFNQILSYADDTDLIGRTIRDVRTAMEGLANESKKIIGIPNAFLGQNLNHEDTNQARKFYQNVNKERKILAQPTEGVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGW1</accession>
    <name>T1GGW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02142240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02142242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGW1"/>
    <dbReference type="STRING" id="36166.T1GGW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002642-RA">
        <property type="protein sequence ID" value="MESCA002642-PA"/>
        <property type="gene ID" value="MESCA002642"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106531_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF35">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="42"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002642-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="181" mass="20307" checksum="AC10CE98DABAA652" modified="2013-10-16" version="1">MEKSQSEVVEVINFSKVEEPYKKYSYEEAIERVGVGKFHYALLLIAGLCFMACITEIASIGQIMFSIKCDLKLTLTQQGLVGSAAYIGIVISSHFMGFLADTWGRTRSLRLTLFLSLCMSLISIMSVNVWMLFTFRFLTGLFISGGQAIVFTLVGEFHGSKTRGNKNKCIKQIKIIDLQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1GH74</accession>
    <name>T1GH74_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GH74"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002764-RA">
        <property type="protein sequence ID" value="MESCA002764-PA"/>
        <property type="gene ID" value="MESCA002764"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046719_1_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097629">
        <property type="term" value="C:extrinsic component of omegasome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097632">
        <property type="term" value="C:extrinsic component of phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035032">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035014">
        <property type="term" value="F:phosphatidylinositol 3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016240">
        <property type="term" value="P:autophagosome membrane docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13664">
        <property type="entry name" value="BECLIN 1-ASSOCIATED AUTOPHAGY-RELATED KEY REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13664:SF0">
        <property type="entry name" value="BECLIN 1-ASSOCIATED AUTOPHAGY-RELATED KEY REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10186">
        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002764-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="401" mass="46324" checksum="3840885B6CF90F36" modified="2013-10-16" version="1">FDNRQRKYAKLQASLKDLNDSYERRIAPRKTLEDVEFCIKEKQDKIDRLKKLIKGKKDLVAARTKYRDNLKKENAEKKKDKLPVYKSNVNQMENRVSDKHVQNQMIRKEHQKLLEKLKETACIDIKQLIDYIFPIKEIVLKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALADARNTSYIGGKWILRESGICEMQYQIVAPSLPANGDYAAYVNWLMESKDDVPKSSPNEIFPSRVAAFRIVGALTYTTQLVNVLSFYLNIHLPHKIAYADFCKKLNEEYFERKVFRLNSNILYLAYTQGVNLSKCKEKNTLENLLTVLNPEISNLGYFGANDVSNAPLMQSMESILFGVETESESEDPTIPMIAYILLRLRIPRVLHDLDVMLAHDFHCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHF0</accession>
    <name>T1GHF0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02102360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHF0"/>
    <dbReference type="STRING" id="36166.T1GHF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002846-RA">
        <property type="protein sequence ID" value="MESCA002846-PA"/>
        <property type="gene ID" value="MESCA002846"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040969_0_0_1"/>
    <dbReference type="OMA" id="PINDCEI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052600">
        <property type="entry name" value="Nuc_rcpt_coact/corep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295:SF6">
        <property type="entry name" value="NEOSIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="24"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002846-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="377" mass="42864" checksum="9F6BFD7715A930CF" modified="2013-10-16" version="1">MKMADSRPEGNNLALIKDPTLAKSRIFLGNIPVCTKEELESIGGPYGTVKASMVKDKFAFLQFESEEIANKAAKALHKSTFKGKPINVRNAGYPKPAVSKPQIPAASSIQPPPIMSNNPDVNDCEIIVVNKNNTEYAEYIEDHLKRLNLRVDLLFPNEEVPIGKRMSYAVLVTTEHQQNRTINVSVLYGEPTEHKNMNVEDGINLIYEDFKSNQKLPQMPPQPQVVTRPPPTQYPHFHPHSHPHPHSHPQRNYSANKHPEPMQVLLNLLANNMSLTVSQYDAILKYLREKREEQVTAELGENAHIPDPEIEMQKKLMNILTKPSVAETHFDLLYPDVEALKDDFRLVELLKDHRVQRAIDSVMKSSLIETIEKHMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHK0</accession>
    <name>T1GHK0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02011293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02011294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02011295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHK0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002904-RA">
        <property type="protein sequence ID" value="MESCA002904-PA"/>
        <property type="gene ID" value="MESCA002904"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909069_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002904-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="15956" checksum="EE73B620D4DD2BB9" modified="2013-10-16" version="1">MKELNIKLGLIQISNLNIINNINEFIDVIEFIDFSFAFELLFKPNKKYAIVVLSHAWYTFNAPLMRFFRNDYYIDSHFYIAFSFVNLAYRYDSILKERLDRQNRWSDGLGLLLTYISDRFFIDNYIYGICAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHU4</accession>
    <name>T1GHU4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02093430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02093431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003005-RA">
        <property type="protein sequence ID" value="MESCA003005-PA"/>
        <property type="gene ID" value="MESCA003005"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309190_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003005-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11715" checksum="6877FD8681CFD936" modified="2013-10-16" version="1">MVINEIQKTSSKYSGTYKNINQNSEKFNFHCPHGWTLSIIYLFTQLLSIFFALLCDFLTFLTLTAISLFRQDGFFLFLFSGIDLQIRLEEFPHSFPRTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHY1</accession>
    <name>T1GHY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02142322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHY1"/>
    <dbReference type="STRING" id="36166.T1GHY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003046-RA">
        <property type="protein sequence ID" value="MESCA003046-PA"/>
        <property type="gene ID" value="MESCA003046"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909074_0_0_1"/>
    <dbReference type="OMA" id="QMQIEGD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397:SF3">
        <property type="entry name" value="DNA DAMAGE INDUCIBLE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397">
        <property type="entry name" value="SODIUM-GLUCOSE COTRANSPORTER REGULATORY PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="1">
        <location>
            <begin position="89"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003046-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="14871" checksum="536C92EB0D4824EA" modified="2013-10-16" version="1">MVQMQIEGDFLASSFSVLEEQPMDMLLGLDMLKRHQCNIDLQRNVLRIGTTGTETHFLSESELPECARLTDRIDEFLNDGAGSSNEISGLSEIDITEIVSMGYERNDVIAELRKHNGNKRLAIAALIAKSIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GI26</accession>
    <name>T1GI26_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02141557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GI26"/>
    <dbReference type="STRING" id="36166.T1GI26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003093-RA">
        <property type="protein sequence ID" value="MESCA003093-PA"/>
        <property type="gene ID" value="MESCA003093"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1429537_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003093-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21274" checksum="FF054DFAC66FD7B2" modified="2013-10-16" version="1">MVLKQSNKSILCGGTLIGANIVLTSASCRDSEPLKYQTRTVYKIVLHPKYQKTTLDNDLAVIVTENEFQIDGQNDHISPLCIEFGNLSSLERCVTLGWGNEAIKYHITDSIMHYANTTELDLGYTLHPVNHCAKIDLDPSLVDMGSGLACTVDDIHYHLKGVFTKVRSDGYLEFTRPDMEWISYLFSGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIF9</accession>
    <name>T1GIF9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Laminin G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02028604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIF9"/>
    <dbReference type="STRING" id="36166.T1GIF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003237-RA">
        <property type="protein sequence ID" value="MESCA003237-PA"/>
        <property type="gene ID" value="MESCA003237"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_410058_0_0_1"/>
    <dbReference type="OMA" id="PAVESHC"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="296"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003237-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="656" mass="70726" checksum="1B6991FEDC4B1023" modified="2013-10-16" version="1">MTVQSTGYLADGKWHNLTLHTSNRILRILVDSRNVGEELVLAGVHDFLDPYLTVLSVGGVRRDIIEMPTETFEGCLANFTINNEIQPFNGSGSIFKDVLSRGRVYQGCHGLTGIGSAQVADPLSIGVTLVIVFFVILLVAILVSFVVFRFRKQQKEKSGGPVGHNGIQSKQNGSSTLLGGSGDSVISRGGLHSGDSNVGYHADNGDILRGMRPELISKKFKEREIANGGSAPEPQRPQRPDIMKEKFPIPPIPLPSHGVDLNEFPEHYDLENASSIAPSDIDIVYHYKGYREGGMRKYKATPPPVSYAHHKHQTSAAQQTHRHSPHHAGPFPPRIPPQTSQPPLPPSSTPRQHQSTPLARLSPSSELSSQQPRILTLQDISGKPLQSALLATTSSSGGVGKDALHSNSERSLNSPVMSQLSGPSSSASRQKPVVPQQSSSQNAMTLTPEEIERLNIRPRTSSLVSTLDAVSSSSEVPRGPGVGSSYHHSADVDAHSATSTDESGNDSFTCSEIEYDNNSISGDKYNKSGSEENRTRDNGPSKQMIAPPSYDGFDSSFRGSLSTLVASDDDLSPHMSPNGASSPSTTTLGWDYLLNWGPNFESLVGVFKDIAELPDSVSVADSDHPATPIRLQSGGKKASEEYDLFKLYVKEDVVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIG7</accession>
    <name>T1GIG7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Caspase family p20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIG7"/>
    <dbReference type="STRING" id="36166.T1GIG7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003245-RA">
        <property type="protein sequence ID" value="MESCA003245-PA"/>
        <property type="gene ID" value="MESCA003245"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036904_5_2_1"/>
    <dbReference type="OMA" id="VCYANTP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF8">
        <property type="entry name" value="CASPASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="9">
        <location>
            <begin position="136"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="8">
        <location>
            <begin position="292"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="44227" checksum="915E786044DE5F59" modified="2013-10-16" version="1">MDQFDRSNILSNINFLVASTKYDILMNACIQYPPNGNHFFFSNNMKKDIENQYKTIEERHKALFKKITQRGPSAYEILKDILKANNFTSAIDKLNDADQKLKPLDFVCESTVSNIKKSAQIILTSNIGVYPMTKKKKGVFFLVNITKFEFNVGSERRGSHFDTDKLISLFGELDFYIFKEVDITKDQFLRRLSMILDSKYCQEAQCFAMALMSHGQMKDRKDAYVTFYDNQREEIHNILDNFSNKTCVHLAKKPKIIFFPFCRGESIDIGVPDPSLSDETEQDAALFNTPILPALSDMLICYGSLPGFVTNREPTEGSWYINALYEVFVNHAHDTHVEDMVKMVGPKIEELYAHIKNGSRQTPIFWNYAFNKHLFFNPGQYTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GII0</accession>
    <name>T1GII0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GII0"/>
    <dbReference type="STRING" id="36166.T1GII0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003259-RA">
        <property type="protein sequence ID" value="MESCA003259-PA"/>
        <property type="gene ID" value="MESCA003259"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2239677_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003259-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="105" mass="11782" checksum="4BA98F93164D1ADC" modified="2013-10-16" version="1">MGVTPDTTCPLSFKEQFQFKSSLMQLVKVCTTIALGAGPMLHILNCETSKDMSSKLKSVYEQKSEASINFLQQQFFGFNKDPYDSVVTHVSKLEKLVKQLKDLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIQ8</accession>
    <name>T1GIQ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02393209">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIQ8"/>
    <dbReference type="STRING" id="36166.T1GIQ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003343-RA">
        <property type="protein sequence ID" value="MESCA003343-PA"/>
        <property type="gene ID" value="MESCA003343"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504275_0_0_1"/>
    <dbReference type="OMA" id="CINIAEW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003343-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="86" mass="9200" checksum="CA89560EC9ABAE19" modified="2013-10-16" version="1">MDNMDETGTRGGGGDSGDILGGDVNIPETLNETAITKINNTGGGGAQAKRPQRRGGKAIPDRPQRALFFLTTENPLRKFCINIAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIT1</accession>
    <name>T1GIT1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02041762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIT1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003366-RA">
        <property type="protein sequence ID" value="MESCA003366-PA"/>
        <property type="gene ID" value="MESCA003366"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1290300_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003366-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="214" mass="23459" checksum="5296A8BC0B242679" modified="2013-10-16" version="1">MYGITITISSSSLTIVFIINILFFVALRLSCVFEMGRGFGINRRGSKPFNQHGHHAHHSRSKLSQTRSNADMPSNLERGNPYSEEGCIHGISINSVCEGDALSSRPFEDNISNAKSGDCSPIWSNDSIMGMKAENRSTPSPITITENRKLTTPEFFDGIFPIKGMSVGAEVNMEKPSVKNNDNDAKILSALQDICSKCDVQDKCIAGEKFASDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-10-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIW1</accession>
    <name>T1GIW1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Prominin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIW1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003396-RA">
        <property type="protein sequence ID" value="MESCA003396-PA"/>
        <property type="gene ID" value="MESCA003396"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_708985_0_0_1"/>
    <dbReference type="OMA" id="SHINEMR"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF1">
        <property type="entry name" value="PROMININ-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003396-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="47954" checksum="7BFB514EBE0BF8FB" modified="2013-10-16" version="1">MINEVSSNLTEVFAKQSVDIDKNSKGYADYGERYINQYGSYRFLFGILVSSVLLTILIFLTCALFCGICGKRPDGYGDDCCNKGSALAVVALVHFLLGLVLHRGICVPLKNPESDDVFSYVDNIIDLNNVFKNNIQSYKTEKSNVIGELPPFRISDVITACKQNKTVYEVLRIENFFSLSDIKDSKEKKRLEAQLDDYVNNLSFTSSIQILQPEDKKKLENLQKSDLVNFDNDKFKDNVNTQITSQSLTELSTKLREAAKNLKGEQQYSEIKITLRNSALHLDTYQHNLVEPMTKQARELLDLSTKLDNILRKDGKPFNENMDIIIKELEQAQTFISERGSTFVKDTLIELKNHLIGQIDNYIKFIVDSIQNDVGKCGPIANVYQSGVTAVCNKFVDPLNGFWAGTAWCVILFLPTIIICVKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJ69</accession>
    <name>T1GJ69_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02010468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJ69"/>
    <dbReference type="STRING" id="36166.T1GJ69"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003513-RA">
        <property type="protein sequence ID" value="MESCA003513-PA"/>
        <property type="gene ID" value="MESCA003513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779556_0_0_1"/>
    <dbReference type="OMA" id="FGEHCLH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053100">
        <property type="entry name" value="Cytochrome_b5-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16740">
        <property type="entry name" value="CYTOCHROME B5-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16740:SF1">
        <property type="entry name" value="CYTOCHROME B5-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="146" mass="16752" checksum="8976E5670A0DD48D" modified="2013-10-16" version="1">MVLTADVSILTVLFRWLLIVSMSSLIFHLIGLNAAHHDPEIFHEGDAHREDRDWGIFQLDSIIDRRDLKGSHFLVLTHFGDHILHHLFPTMDHGVLQQIYPILFETMDEFGVGIRDQSYLSHLIGQQKQLNRMSPNPIPPGGKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJH4</accession>
    <name>T1GJH4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02199453">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02199454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJH4"/>
    <dbReference type="STRING" id="36166.T1GJH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003622-RA">
        <property type="protein sequence ID" value="MESCA003622-PA"/>
        <property type="gene ID" value="MESCA003622"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1798659_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003622-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="144" mass="16303" checksum="94806519B885DC6A" modified="2013-10-16" version="1">MECGKGFYRKDHLRKHTRSHIARRVKAELTQNQQNGSTSESGGPSQNQVQHNGQQIMQQTQIQQLSTSSVFVFTVVDRRYYNQPQKTPLALLEILRNASEGNILLVFLRSLKDIFNIFCICHGASNTRDSFSISISKSSESVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK06</accession>
    <name>T1GK06_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK06"/>
    <dbReference type="STRING" id="36166.T1GK06"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003816-RA">
        <property type="protein sequence ID" value="MESCA003816-PA"/>
        <property type="gene ID" value="MESCA003816"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2852021_0_0_1"/>
    <dbReference type="OMA" id="RYESMRV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031833">
        <property type="entry name" value="DUF4748"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15932">
        <property type="entry name" value="DUF4748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003816-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="71" mass="8087" checksum="2A57F589A1EA7E2F" modified="2013-10-16" version="1">MGGGPENSMRLGSFGKIAISWGVLTGVGIYSFILSKNSIDKQRLESLRVRDRMRKSNYGDYQVEEDRKFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK61</accession>
    <name>T1GK61_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163326">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02163327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK61"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003878-RA">
        <property type="protein sequence ID" value="MESCA003878-PA"/>
        <property type="gene ID" value="MESCA003878"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443370_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003878-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="10419" checksum="C140D75E744ED4F9" modified="2013-10-16" version="1">MKRMEDDYKDLILRAMRRTRAKARYSDESKRLAEERSTLTDLLDQKGPKGIKYSQVIRKIKPAQNVKFSDEPPKVHKILAVTMSKAKASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GK87</accession>
    <name>T1GK87_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02107386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GK87"/>
    <dbReference type="STRING" id="36166.T1GK87"/>
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        <property type="protein sequence ID" value="MESCA003906-PA"/>
        <property type="gene ID" value="MESCA003906"/>
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    <dbReference type="OMA" id="TDMATER"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="199"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003906-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="257" mass="27384" checksum="CB05F47764989CE0" modified="2013-10-16" version="1">MPSAPGTSTAQINRQQQIATIETQPSNPMPIPIVTCDLASDISTSEAGELEAHYYANTATTTNNNYSPASSDLNKPSVDTLKVSTIASTHNQITSNLSLPATSSTPNLLVPGESAGMAVSAAATTTVSSINNNGNSGGSSSKELRISPVPMRCRALSVGIDTDMVSEFDPSSSDSGVVSRCAVVKPLKFRLCQPFLGRNRNNQRRNESKAAITSNSNKIVGSKPSETAELEPAIPKVQKQRDPEKEKRRIARKKEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKU5</accession>
    <name>T1GKU5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02008091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004126-RA">
        <property type="protein sequence ID" value="MESCA004126-PA"/>
        <property type="gene ID" value="MESCA004126"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3052735_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004126-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="54" mass="5820" checksum="467C2A4AE9C5485F" modified="2013-10-16" version="1">MLRLAPICLEKHQDEEALPTRSHQLSAVPSAITLNINEHSGNGVVGVKKTLSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GL71</accession>
    <name>T1GL71_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02099054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GL71"/>
    <dbReference type="STRING" id="36166.T1GL71"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004264-RA">
        <property type="protein sequence ID" value="MESCA004264-PA"/>
        <property type="gene ID" value="MESCA004264"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431367_0_0_1"/>
    <dbReference type="OMA" id="RNIESCP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="22601" checksum="9F626994560A7EED" modified="2013-10-16" version="1">LNLRPNSELREKLAIAREDFQFREVVSSLRNKYEPVHNGDQLYVPEESIDNVEEQDYNLYLPPWICQPYIRPAPEVHIKTIEEKVKIAEDREKRKYFDDEGNEISRKKMKRLKKKSRRPLKPEGAHERNIESCPVCTNPLGFKCVFKLCRNCCREKCNTEIFDCIGHKFYTKTKLEKKKILREKALKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLG5</accession>
    <name>T1GLG5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02155854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004367-RA">
        <property type="protein sequence ID" value="MESCA004367-PA"/>
        <property type="gene ID" value="MESCA004367"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2796893_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004367-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="68" mass="7784" checksum="A57C49ED5F8C01FE" modified="2013-10-16" version="1">MIRYPNRMEFLDSAHPVFANRVYLHRDGLFVFLISPFAVSASQKPQGQMFVPLQHIASVGYQHPNPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GM69</accession>
    <name>T1GM69_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02188430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02188431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GM69"/>
    <dbReference type="STRING" id="36166.T1GM69"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004637-RA">
        <property type="protein sequence ID" value="MESCA004637-PA"/>
        <property type="gene ID" value="MESCA004637"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1995216_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF76">
        <property type="entry name" value="FI02861P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004637-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="125" mass="14327" checksum="E20081195DAC71AA" modified="2013-10-16" version="1">MIAARSKALKRLQKNKDIHESVFLQNLMAYCSKDANSCVEKAAKICLVGLRKIEVDSKGRMLLSELRNAIIQDLKSGLTPFFVLATRRKKFSRQENVSLQTRMDIERKLDIEGHWKLFADVICSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMJ3</accession>
    <name>T1GMJ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02081587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02081589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GMJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004768-RA">
        <property type="protein sequence ID" value="MESCA004768-PA"/>
        <property type="gene ID" value="MESCA004768"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402158_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004768-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10301" checksum="E212380CBA4F6D58" modified="2013-10-16" version="1">MIEIKERSCFVHIDVPGHDDGADPLPDSAASVIENFKNKFTTWKTDEVSQSAESFLMYHKFGHVNLVTILDFLHVKYVIGLGEGAGANVLARX</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GMK7</accession>
    <name>T1GMK7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02160931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02160932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GMK7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004782-RA">
        <property type="protein sequence ID" value="MESCA004782-PA"/>
        <property type="gene ID" value="MESCA004782"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2309202_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004782-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11392" checksum="5F7BE37E4DE02B93" modified="2013-10-16" version="1">MLKELRDRDRHHLFIARYSAFQFGVSYAFRKSPINCNTGFPRTSLFFGNASFVDFVCKRSPLRTANSVGFNPVVGDDINSLTVASLVLNHARIYEHCKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPG5</accession>
    <name>T1GPG5_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPG5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005495-RA">
        <property type="protein sequence ID" value="MESCA005495-PA"/>
        <property type="gene ID" value="MESCA005495"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1385618_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005495-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="197" mass="21587" checksum="7F5E5589FCC241E3" modified="2013-10-16" version="1">METNRKFIDDISTTGDLIDLRNRKYSSTTDKSPQLTSSSVKTSASVRIPHLLNSSSSDCRKIFSTEANSSTMTTMAGNANVESNLNSKQSSQNSFLDGGDGFNSTTDSKLTSEDNCSSDMNVKLISSEDDEKDVKDGKEKLNLNLNLKTQITPGRSIGTVDKTDLRSSANSLWSKSDSEGAKMFENFNRNLIKAIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQA9</accession>
    <name>T1GQA9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02112658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQA9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005813-RA">
        <property type="protein sequence ID" value="MESCA005813-PA"/>
        <property type="gene ID" value="MESCA005813"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2765149_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005813-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8037" checksum="69AA262C46B1CB5D" modified="2013-10-16" version="1">EPWVRGRLIQGLHHHELNLFYKVPDENGRPRACILIRKNINAFLLTAYCDDDTTSVVLECSNNWITVVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQD3</accession>
    <name>T1GQD3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
            <location evidence="1 8">Extracellular space</location>
            <location evidence="1 8">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02189266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02189267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQD3"/>
    <dbReference type="STRING" id="36166.T1GQD3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005837-RA">
        <property type="protein sequence ID" value="MESCA005837-PA"/>
        <property type="gene ID" value="MESCA005837"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2560903_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005837-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="82" mass="9332" checksum="79070B92E51D49A5" modified="2013-10-16" version="1">MKLVIYLQSPDAIVALGAGHMMGAKECQHQFKGHRWNCSQVWHKNVFGHVMFVVLRSVMKLAPIALKRSTKMTANSLFLFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQF1</accession>
    <name>T1GQF1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02162618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQF1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005856-RA">
        <property type="protein sequence ID" value="MESCA005856-PA"/>
        <property type="gene ID" value="MESCA005856"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2402171_0_0_1"/>
    <dbReference type="OMA" id="NITQNME"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005856-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10937" checksum="E017980F2F5073F2" modified="2013-10-16" version="1">MKATSDIRIEKDKLNEPVYDGRSPEWPLSIAQFKETKEMFKYDDHIDNNIRLNKALKGTARSLIGPLLIHQMNITQNMEDLKEHFGRPERKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQH4</accession>
    <name>T1GQH4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Suppressor of hairless protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Su(H) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02196789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02196791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQH4"/>
    <dbReference type="STRING" id="36166.T1GQH4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005881-RA">
        <property type="protein sequence ID" value="MESCA005881-PA"/>
        <property type="gene ID" value="MESCA005881"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022207_2_1_1"/>
    <dbReference type="OMA" id="QRQDMPH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990433">
        <property type="term" value="C:CSL-Notch-Mastermind transcription factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01176">
        <property type="entry name" value="IPT_RBP-Jkappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1450:FF:000001">
        <property type="entry name" value="Recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000003">
        <property type="entry name" value="recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000074">
        <property type="entry name" value="Recombining binding protein suppressor of hairless, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1450">
        <property type="entry name" value="LAG1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015350">
        <property type="entry name" value="Beta-trefoil_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036358">
        <property type="entry name" value="BTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040159">
        <property type="entry name" value="CLS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015351">
        <property type="entry name" value="RBP-J/Cbf11/Cbf12_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037095">
        <property type="entry name" value="RBP-J/Cbf11_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038007">
        <property type="entry name" value="RBP-Jkappa_IPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10665">
        <property type="entry name" value="RECOMBINING BINDING PROTEIN SUPPRESSOR OF HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09270">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09271">
        <property type="entry name" value="LAG1-DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20144">
        <property type="entry name" value="TIG_SUH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01268">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01267">
        <property type="entry name" value="LAG1_DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110217">
        <property type="entry name" value="DNA-binding protein LAG-1 (CSL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RBP-J/Cbf11/Cbf12 DNA binding" evidence="12">
        <location>
            <begin position="148"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-trefoil DNA-binding" evidence="13">
        <location>
            <begin position="281"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="553"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="561"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005881-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="70055" checksum="3371EE044E4B1737" modified="2013-10-16" version="1">MKSVNGEPQQHHHNHHYYPSSSPSSSSAAHHHQNHQHLQTMPHYGLGGGYVQTPPSPPTQQSGQGGGPGPGHLGNSALLPTSPSHHMGLMSRMNYTNNQSPSGPGGGPGHGPGHNPGSQSIYSSRTFEEKSLTREAMEKYMRERNDMVIVILHAKVAQKSYGNEKRFFCPPPCIYLLNDGWPRRQEEMLQNGESEQGSQLCAFIGIGNNSDQDMQQLDLNGKSYCAAKTLFISDSDKRKHFMLSVKMLYGNGHNIGLFNSKRIKVISKPSKKKQSLKNADLCIASGTNVALFNRLRSQTVSTRYLHVENGHFHASSTQWGAFTIHLLDDNESESEEFAVREGYIHYGATVKLVCSTTGMALPRLIVRKVDKQMALLEADDPVSQLHKCAFYMKDTDRIKNYSVPGYSVSKEPNKEMINDGACWTIISTDKAEYQFYEGMGPVAAPVTPVPIVNSLNLNGGGTAAILELTGENFTPRLQVWFGDVEAETMYQCGEVMLCVVPEISKFRGEWLWIRQPTQVPISLVRKDGIIYSTGLTFTYTPELAPRPHISPVEDILRPRHNNNNNSSNNGSTNTNSTNSTNNNMNSMTNNNPSSMGNHQQSPSAPSAPTSSTPQQQQQLPSMSDVQWNSHHGGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQJ9</accession>
    <name>T1GQJ9_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02038487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02038488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQJ9"/>
    <dbReference type="STRING" id="36166.T1GQJ9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005908-RA">
        <property type="protein sequence ID" value="MESCA005908-PA"/>
        <property type="gene ID" value="MESCA005908"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_596267_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005908-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="459" mass="51313" checksum="D84689E7827EDE4D" modified="2013-10-16" version="1">MGDEDEDERDEDELDGEEEDDEEDADEDEDIEDEEDEEEDEEDDEDDMNQENPEESLRKLPECLEILSNVALKPDEFTRALSEMRPTELVQVFPQITNFISQKPQQINIPQQIGFQNGKNQGNYTHEEHFRMYHPVVDQLDEEALATIYPALDETFNTDGTINVDVGGDGEEEDETKNYEFGLESVEEFFNVFQNCGDPSSIPDCPEFAAELTTMNSFCKTRWNGTGLLNQLQQWMLEEPQYADENIQHQQQKIAANIVLDAPLQRLSDSSASSQAQEDVTIYSSNAGPLHIQQQGSNQQQIHLPLIPITTQEGLTEVLRNDEADDSSLPTSAPNTKNEQEGDVSSQDQPLDLTTGQPQQPSSSHLIPGVNEDGSLIFDVDAGRTSPRSLQFLFQLPNVQQQSNLQQQQQPQAQQVIVKNNTISSNSVDVNSATSPGYPTSSSGYTNAATITNCCKAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQS9</accession>
    <name>T1GQS9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02177930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQS9"/>
    <dbReference type="STRING" id="36166.T1GQS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005994-RA">
        <property type="protein sequence ID" value="MESCA005994-PA"/>
        <property type="gene ID" value="MESCA005994"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_2_1_1"/>
    <dbReference type="OMA" id="DWAYSEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000084">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF123">
        <property type="entry name" value="PEPTIDASE M14 CARBOXYPEPTIDASE A DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="12">
        <location>
            <begin position="125"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005994-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="424" mass="48813" checksum="C5252E751EC04BFF" modified="2013-10-16" version="1">MNQFVFKTEREMKCSLGALFVICFISSVYAEKVRYDNYKILNLKLNNLDKVAFIDKLKKLPAFSFLRNIEHNQDVLLAPKELQKLAPLLKKANISSEILIDNIQEIIDDEEVKTKAAGDTFTWDRYQSYESIQKFIDGIIEDHPDVVTPFVIGKSYEGREIRGIKISYKEGNRGIFLESNIHAREWITSATCTYFINELVNSEDPDIRYIAENVDWYIIPVFNVDGFVYSHEKDRLWRKTRQPNTNPECPGADPNRNFDHTGITMKDLQEIHVMKHLEVFLLFLNPNVKLFLIIGIKDKIGVYLAYHSYGQYILSPYGHSKTEFPPNYNDIQEIAKSSYDAILAQGYGTTYRYGSTGSVLYIASGSSVDYTYNELDIKIGYTFEFRDQGRYGFVLPSINILPNCQETVAAMIALVKKAQELSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRJ3</accession>
    <name>T1GRJ3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02061126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRJ3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006278-RA">
        <property type="protein sequence ID" value="MESCA006278-PA"/>
        <property type="gene ID" value="MESCA006278"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_475136_0_0_1"/>
    <dbReference type="OMA" id="WHHFCTS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052607">
        <property type="entry name" value="CEP104-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13371:SF0">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 104 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13371">
        <property type="entry name" value="GLYCINE-, GLUTAMATE-, THIENYLCYCLOHEXYLPIPERIDINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006278-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="574" mass="64585" checksum="69A9B298FE84A27D" modified="2013-10-16" version="1">MAVNILGVFVDDIDSTDCLKIYEDVEPQLQSVCDDLLFSMYVEESIADIIRELEVKKVEAVNKERFEYARKIKLCMSALRTAGERLGRYALAKRQAIQVEDFIAAKLRKEQIDIYRTAILKHLLVDELLEKNGILPENDISNEIYSSKPILPSAPSLQEVALGITFESILTTRNNQEDKSSTDGNSSVSTTGASLNEVISNHNHLLKQHEVPCSPRLNACRSPVCSSPLSSRHSSLRRRNKSAPRNSYEEHDERTIPTLRQECQGAAILDADPSRGRSKLSDRERRQASLSILIFGNELVELFYSRQFQDREEGLTRLRNFLKEQLKNDSLCGVNKIARATTMLLHRSIRAPVFFIDYIPNKVSANELSRSVDKLLPELLARSGDPSPRIHTLAQHTILTIAACSYVQEQHLVAPSLSRPVGSGTHPRLALSRIQMLEQLVLNQGISLDKHSGLTCRSLTEAGSSGLHHPTESVRKVAERILILVYKVNPRLVRKQLPPDDDITRRNLLYRQLFSEFDKIDASKKMGNSNKDNSQCQSPSPNSSSEKSLSKWKYNSLSDSKNDGNNDKIKEGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRN5</accession>
    <name>T1GRN5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DML1/Misato tubulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02057884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRN5"/>
    <dbReference type="STRING" id="36166.T1GRN5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006322-RA">
        <property type="protein sequence ID" value="MESCA006322-PA"/>
        <property type="gene ID" value="MESCA006322"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022511_0_0_1"/>
    <dbReference type="OMA" id="FVGSHIW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06060">
        <property type="entry name" value="misato"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DML1/Misato tubulin" evidence="1">
        <location>
            <begin position="9"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="389" mass="45217" checksum="C0F37E54F04D1413" modified="2013-10-16" version="1">MEEKEYKLNENVKTWTDFLYSRYHPRSINIVRDYKHSDEKESLDTFSNGKSLWKTDLFEDEFCDRIRQYVEECDFLQGFQNIFDISNGFSGLSMNCMEYLNDEYAKSILAIPVFSPKNVIYENADEAMSNAIRIVNIALTYNGLVENSSMFLPLSLREREWKTPGVSRQFPHLTYKEDNLYQTSAIIASYLDTLSLKYRFKNAASYLPGFCTDMTNYGRKLTAGAFSLPLQMNSEEDLIDCLDRFDGDLFTQISPNTQIGTDYVVQSVFVRGVPKNRLKRPMQSAKLQMRMAAYRCDSVSEMFQLYFQCKNHASLAHVAACPRLLPTLATVQNSGDIADTLETLHREAERIKIKKIHRFRESGFEEDEYGEMLENLLQFKDNYEDNFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRQ4</accession>
    <name>T1GRQ4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyridoxine kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02135534">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GRQ4"/>
    <dbReference type="STRING" id="36166.T1GRQ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006342-RA">
        <property type="protein sequence ID" value="MESCA006342-PA"/>
        <property type="gene ID" value="MESCA006342"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046496_4_0_1"/>
    <dbReference type="OMA" id="YKLMRGQ"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006342-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="15421" checksum="F0F77D9ED4618224" modified="2013-10-16" version="1">MSSCNIAGQILRNNMESSTIKRRVLSIQSHVVHGYVGNKSATFPLEVLGFEVDAINSVQFSNHTGYKVVKGQVLQEKELADLFEGLEKNNLLQHYSHLLTGYIGNASFLRQVSAIVKKLREINPNLIYVCDPVMGDNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS12</accession>
    <name>T1GS12_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartyl/Glutamyl-tRNA(Gln) amidotransferase subunit B/E catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02169431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02169436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS12"/>
    <dbReference type="STRING" id="36166.T1GS12"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006459-RA">
        <property type="protein sequence ID" value="MESCA006459-PA"/>
        <property type="gene ID" value="MESCA006459"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_1_0_1"/>
    <dbReference type="OMA" id="TCEGKMS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartyl/Glutamyl-tRNA(Gln) amidotransferase subunit B/E catalytic" evidence="5">
        <location>
            <begin position="21"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartyl/Glutamyl-tRNA(Gln) amidotransferase subunit B/E catalytic" evidence="5">
        <location>
            <begin position="198"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006459-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="301" mass="33739" checksum="25C199619BE19F97" modified="2013-10-16" version="1">MLRIISRNYSTKPIKSKEWKTVVGLEVHAQIQSNSKLFSGSQISFNAPLNSRVSLFDAAIPGTLPVLNERCVEAGIKTASALNCKVNSLSMFDRKHYFYSDLPTGYQITQQRAALANNGVIKFPVFTPGVNKKPYTKEEFGGFKQSRNSLMELVFDHTLEDGDEASSLVKELILILKSLGTCSGKMEGKSNQFRGGGEINRQIEIKSGGGEITNETRNWDATLRKTVAMRDKEIQQDYRFMPESNLPMLLVDTEPDDKIRGNFKLKRIVSDLPKLPAETRKELVENFGLPEETAIIIVVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS99</accession>
    <name>T1GS99_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02392437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS99"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006550-RA">
        <property type="protein sequence ID" value="MESCA006550-PA"/>
        <property type="gene ID" value="MESCA006550"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2148697_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006550-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="112" mass="13181" checksum="F8BC57E2F005F038" modified="2013-10-16" version="1">MDEQSEVFTLKYRECTPEDYLCIYKHRELFHNEVQHDEGDYVKPIGILGMMCPCLRSCNTNVGGLVSLFMGASLTSFTEATYYILYATIKFIRGRIHHRMNKDVEYNHIKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSJ8</accession>
    <name>T1GSJ8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02087043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02087044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSJ8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006658-RA">
        <property type="protein sequence ID" value="MESCA006658-PA"/>
        <property type="gene ID" value="MESCA006658"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2760704_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006658-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="70" mass="8224" checksum="8D76FD6C5084D07E" modified="2013-10-16" version="1">MRQGCSSYVRTCLWLVFRQEKTERMALFRVRSALYESSQESRDDGQSSNFCQIVVRSVEITIKVPSFVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GST8</accession>
    <name>T1GST8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="9">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="9"/>
    <dbReference type="EMBL" id="CAQQ02178313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02178314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GST8"/>
    <dbReference type="STRING" id="36166.T1GST8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006749-RA">
        <property type="protein sequence ID" value="MESCA006749-PA"/>
        <property type="gene ID" value="MESCA006749"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145020_0_0_1"/>
    <dbReference type="OMA" id="CAAYMAK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008545">
        <property type="term" value="F:JUN kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000126">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF15">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="80"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006749-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="243" mass="27595" checksum="243C68DC4E6983D4" modified="2013-10-16" version="1">MEKNSNHCTNHNALAPPRSNILCWMLTKDRQHKTSVPIKPRTEFSAPAPLQTTRDKIKRQACGTLHIGPTEDYTFTSDDLTDLGEIGRGAFGTVNKMIFKKTSKEMAVKRIRSTVDENDQKQLLMDLEVVMKSYECKCIVQFYGALFKEGDCWICMELMDTSLDKFYKYIYDKKQQRIPETILAKITVATVYALNYLKEKLAIIHRDVKPSNILLNTNGDIKLCDFGISGQLVDSIAKTKDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSV4</accession>
    <name>T1GSV4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02029610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02029611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GSV4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006766-RA">
        <property type="protein sequence ID" value="MESCA006766-PA"/>
        <property type="gene ID" value="MESCA006766"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944364_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006766-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="60" mass="6924" checksum="5ECFD80CF3D961BF" modified="2013-10-16" version="1">MDKFWGEVGGSRNYVQCISRKCEFTMQKRNFAPARNEIIETTISKIKVQARGSKNIKIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GSW5</accession>
    <name>T1GSW5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CAQQ02045087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02045089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GSW5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006777-RA">
        <property type="protein sequence ID" value="MESCA006777-PA"/>
        <property type="gene ID" value="MESCA006777"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830474_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046931">
        <property type="entry name" value="HTH_61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057954">
        <property type="entry name" value="SET_TTL12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF12">
        <property type="entry name" value="HELICASE POLQ-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20470">
        <property type="entry name" value="HTH_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25556">
        <property type="entry name" value="SET_TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="7">
        <location>
            <begin position="17"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase theta-like helix-turn-helix" evidence="6">
        <location>
            <begin position="105"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006777-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36997" checksum="1F8383E28B4777EF" modified="2013-10-16" version="1">DYTDSHREETSPTLTARVCKADGIKASDENELYLVDHAWTFRLNQAKEVLLQHPQLVDRMAAGVNLPAKRVIIRAPYVGSSFMMLSQYKQMIGRAGRAGMGGSCGESFLICSSRDNLKVAQMLTSPMTKVLSSMNDENAIGLQSLILSAIGLEIANCRKDLVALAQTTLLSLQSDEIECECVETTVXXXXXXXXXQSDEIECECVETTVLAILKTMHKHSVWEFQGSKANLDMSVFASAEQSLNTTQAKTKFALCNTTKIQLTKIGKAAFKAGVDFIKAKTVAIELRNSQKCMVLTNYSHILYLVVSFNSNANGSEMFQTNVNILYREYCKLDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTY1</accession>
    <name>T1GTY1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine kinase</fullName>
            <ecNumber evidence="2">2.7.3.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.3" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02082990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02082993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTY1"/>
    <dbReference type="STRING" id="36166.T1GTY1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007180-RA">
        <property type="protein sequence ID" value="MESCA007180-PA"/>
        <property type="gene ID" value="MESCA007180"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019868_0_1_1"/>
    <dbReference type="OMA" id="EYHGFTP"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004054">
        <property type="term" value="F:arginine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000006">
        <property type="entry name" value="Arginine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000003">
        <property type="entry name" value="Three-domain arginine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF38">
        <property type="entry name" value="ARGININE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="10">
        <location>
            <begin position="137"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="140"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007180-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="291" mass="33122" checksum="6FD861AC21707A94" modified="2013-10-16" version="1">MKFIRDDKEEENKSSDCTMVDAAVLAKLEEGYAKLAASNSKSLLKKYLTKEVFDNLKNKKTPSFGSTLLDCIQSGLENHDSGVGIYAPDAEAYSVFSELFDPIIEDYHTGFKKTDKHPPRNFGDVSAFGNVDPNNEFVVSTRVRCGRSMEGYPFNPCLTEAQYKEMESKVSTTLSGLEGELKGKFYPLTGMEKAVQQQLIDDHFLFKEGDRFLQAANACRYWPSGRGIYHNDNKTFLVWCNEEDHLRIISMQMGGDLGQVYRRLVTAVNDIEKRIPFSHHDRLDLRDFSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU13</accession>
    <name>T1GU13_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5'-nucleotidase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02392067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU13"/>
    <dbReference type="STRING" id="36166.T1GU13"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007212-RA">
        <property type="protein sequence ID" value="MESCA007212-PA"/>
        <property type="gene ID" value="MESCA007212"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029966_1_0_1"/>
    <dbReference type="OMA" id="WCSQGNA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000086">
        <property type="entry name" value="LD24878p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF38">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007212-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="377" mass="43662" checksum="685081FBC554B8EA" modified="2013-10-16" version="1">LSLRQTKSTNKLGLPPKRSHIRRFKREHLETGQEWYNSESLSWGKSSHTSEIDSIYGADRKWIASTEFIKNPLNTWNGPYSEQMRTCLDYFDIPISLVMSRAVESLNNCSQDSSPGEYKVWDDILAGLFEMYKREHFNEGSSEYFEALKNNPEKYLLKTDGRVIQWLKDLKEVQGKALFLLTGSDISFANFTASYALGENWKDLFDSVVCFAKKPGFFVENDRPFYRVDLEKLEEGEEVPAGERLRFNITYSQGCWSNLKHLLKEKSGSCEPKTLYIGDNLVQDVLCPTRFANMDTVAISEEMFNEEVPVKKEVKSVFKEVTSSCIWGSYYKFNESDTLWYDIIKKYSKICVPTVDYLVDLKKLDDAQFYPLPPKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU33</accession>
    <name>T1GU33_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU33"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007235-RA">
        <property type="protein sequence ID" value="MESCA007235-PA"/>
        <property type="gene ID" value="MESCA007235"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2471656_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="88" mass="10391" checksum="9B6FCD8CF823AD99" modified="2013-10-16" version="1">MYVQNRLPVWVGLPILEERERKQTMVKTIYQEDFDYVLKENIVDFSMSPVEAKKETIKQFEAQGINLDNIIKSLQINEETGRPFITEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GU92</accession>
    <name>T1GU92_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02157687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GU92"/>
    <dbReference type="STRING" id="36166.T1GU92"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007301-RA">
        <property type="protein sequence ID" value="MESCA007301-PA"/>
        <property type="gene ID" value="MESCA007301"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173848_0_0_1"/>
    <dbReference type="OMA" id="CASTCLE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007301-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12868" checksum="BF81B8E38028DE4F" modified="2013-10-16" version="1">MYRKFWVNEHHHNYQKVLWRFSSKEPLKTYALTTVNFGTSSAPFLAITLLKYIAESDIALNFPLSSKVVKLDFYVDDLVLGSYSCEEVIDIQWQVRELLSNFGFDETDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GV91</accession>
    <name>T1GV91_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02163908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GV91"/>
    <dbReference type="STRING" id="36166.T1GV91"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007681-RA">
        <property type="protein sequence ID" value="MESCA007681-PA"/>
        <property type="gene ID" value="MESCA007681"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2530054_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052069">
        <property type="entry name" value="Ca-reg_mRNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962">
        <property type="entry name" value="CALCIUM-REGULATED HEAT STABLE PROTEIN CRHSP-24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962:SF1">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN CG9705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007681-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9106" checksum="77C3BB89AEC3CC21" modified="2013-10-16" version="1">MSSHEITPEKRPPPSPIPSNLQLPSPIITKRTRTASTSARAMENPIEKGLVKSFSRSKGHGFITPHGGGDDLFVIFQSKLQGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GWK3</accession>
    <name>T1GWK3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02127232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GWK3"/>
    <dbReference type="STRING" id="36166.T1GWK3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008180-RA">
        <property type="protein sequence ID" value="MESCA008180-PA"/>
        <property type="gene ID" value="MESCA008180"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608771_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008180-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9349" checksum="98C622055DCB8B1E" modified="2013-10-16" version="1">MYSQSYKDIERYHKSTRAAIAIKQNTETPEKKARRKERDDQNSTDTKLYLEESVLERKLPDADLKALVDFQLDWILMNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GXJ2</accession>
    <name>T1GXJ2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02189288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GXJ2"/>
    <dbReference type="STRING" id="36166.T1GXJ2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008544-RA">
        <property type="protein sequence ID" value="MESCA008544-PA"/>
        <property type="gene ID" value="MESCA008544"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_856027_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007614">
        <property type="entry name" value="Retinin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931:SF3">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04527">
        <property type="entry name" value="Retinin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577735" description="Cuticle protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008544-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="34593" checksum="B489688EDF62750A" modified="2013-10-16" version="1">MFKLVVLSVLLAVAAAKPKPSTLEVLPLGKTTTTIIEPGRVDVPVVKTALIQEPGTVEIKEPTLTKVGDLVRSVPTAVSHQSSTIVHSKADIVEPIIAQGVRTYQQPIVKTFTAPVIENVPISTPIVRKYETPLLAAPLIKGLEASPLIQTYAANPLYYNNNAFGYYYGYGEPYYRSVLVSALFALAAAKPGFLETHQVEVAPVLQKTVVHEPVATVVKSVPTAVSHQSQTIVHSQPIVKSVVHTPVIKTVHAAPVVHSAPLVHATPVVHSAPLVHAAPIVHTAPVVKTINSAPLVHAYHAATPVVHAAPLVKTIASPVAYTHFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY23</accession>
    <name>T1GY23_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02084494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02084495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="MESCA008743-RA">
        <property type="protein sequence ID" value="MESCA008743-PA"/>
        <property type="gene ID" value="MESCA008743"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1284600_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008743-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="215" mass="24656" checksum="D2CCBFF960ED6783" modified="2013-10-16" version="1">MWFGLSKKIVAVTVTIPKTGYVPGEFIPVEIFINNKSSSDIWELSTKLVLKAIHRTRSPKDTLIKKITLSQVKYSKDRVGKNQEFKDCLKIPPTPPSYEDTCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSESKKSEYFEFENYNPILRPKIEESPFMDSSKFKDTGKEFRLDMKFIPVFPKFNTKLITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY59</accession>
    <name>T1GY59_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02180138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02180139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY59"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008781-RA">
        <property type="protein sequence ID" value="MESCA008781-PA"/>
        <property type="gene ID" value="MESCA008781"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1058813_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008781-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29811" checksum="730D576AF33DF3CC" modified="2013-10-16" version="1">MIRFLEKNQKKIKPVETAVVVPVVPKKPKTNLITAVPSHKVKRLVISLQNSEDEDNELCGDIFTKHFLSTNICLDSENYADAASPSSLFLKNSQKDEITKNYKNLATRLRALKALNKDNVSLTKDCSTQVELEATIPTDNQNTDVQGDNKKLEEIEETEKTPKKVIEEVAATEEKLKEPETLRIDYVIRPEFILGVIQERRQKKGFRILQWSNEYISPLCFRVNSASSSASRKTSQNINPNSIVCPYALIGRCDDKGCKYNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1GY89</accession>
    <name>T1GY89_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02151289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GY89"/>
    <dbReference type="STRING" id="36166.T1GY89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008812-RA">
        <property type="protein sequence ID" value="MESCA008812-PA"/>
        <property type="gene ID" value="MESCA008812"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1113707_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF45">
        <property type="entry name" value="CYTOCHROME P450 6G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008812-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28618" checksum="4D3C526F828946A8" modified="2013-10-16" version="1">PGLFIRDPELIKHICIKDSLHFKSRDSNIENIELSSSKPNFSSDTIKQFFSIFDEIGNNLNRFLSDLTAANGVASTNIKEICEFFTTEIILKVVLQADSKDEKLRNYIKTLSSSNFLKNMHSIFVFFAPNLAKMFKKQSKTSENEFKASSAALSFSLYELAMNAEIQDNLREEIKNVLSENEGLVTYEVINSSMPYLDKVVNEVFRLYPAKPIIVRELSLLDGEDSYNSPPPQKMSSKHHQLRYRLHSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYD6</accession>
    <name>T1GYD6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02134427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYD6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008861-RA">
        <property type="protein sequence ID" value="MESCA008861-PA"/>
        <property type="gene ID" value="MESCA008861"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661608_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008861-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8611" checksum="C4A0654C8AC0F5BB" modified="2013-10-16" version="1">MCPSLGREGWLRDIDNCNRYFRCYRENNQLIQLKMDVTGICQNSPYAGSCSAAGFCSNKQTGYYANPTDVTKSLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYH2</accession>
    <name>T1GYH2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peroxin-19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxin-19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02003564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYH2"/>
    <dbReference type="STRING" id="36166.T1GYH2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008899-RA">
        <property type="protein sequence ID" value="MESCA008899-PA"/>
        <property type="gene ID" value="MESCA008899"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1671336_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008899-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="158" mass="17179" checksum="08FC370C5F9FD902" modified="2013-10-16" version="1">MNKVFGGSAEAPAESQPLSDEQFALGFQKMAEAAAMALQGQTNITDEGQKFSASISEALKGLQQGVENIQAPISEDDVANMFSNLNMAEAGAGEEGNNPFLPFMEGMMQSLLSAEILLPSIKELLDKYPSYLAENGDKISAEDKERFENQHELFKVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GYI5</accession>
    <name>T1GYI5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02149755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02149758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GYI5"/>
    <dbReference type="STRING" id="36166.T1GYI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA008914-RA">
        <property type="protein sequence ID" value="MESCA008914-PA"/>
        <property type="gene ID" value="MESCA008914"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010440_2_0_1"/>
    <dbReference type="OMA" id="HEQWIDS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF106">
        <property type="entry name" value="TRIO, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="12"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA008914-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="613" mass="70514" checksum="A8B196DC316794D2" modified="2013-10-16" version="1">MDGQRARDIQSLLKERIVLLTGGRDRRGGPILSFPSNSKRERLKPEDLRRLLTYLIMIPSDSAKNLRFTVIMDMRGNGNCATNVKTILKVLQEHFSANIHNVVLIKPDNFWQKQRTSISSHKYKFETTTISIDSLNKIAEPNQLSSDFEGMQLYDHEQWIDSRIAIEEFFWHASDLVDRIDDLQEDLNRNDIAENVHGAKERLDYNKEMRKKIEKLPIKELTVEAEKLIFKYSNYQQSQENGSTSRHIPIQANNPDTENALKKASRQLDVIRNGQQHLFSLWQAKDARLSQCFQLRLFEQDCEKMFDWILRNQDTFRANYVEIGMNYAMAKKLQDEHQKFAEASMNVAVNIDRITDVAARLIESQHYAADHIKTLSQRLSIIGSTGLKERSNVLQLSVLFHHKAEQYCNNVNSWAGACLASQQLPTDIKSLEGLIPNHKSLLPTISEAYSDVHSTSKTLLYQLDHLVQVCNQPPPNGVAAYNENMVYQNPAADYSEGASHVLEEDVRQVLDWLKNHGEVFLMKNTGIGRNLQKAKIYKKSHEHFENVAQNTYSNAEKLLNAADNLVGNGEADPDEIFEVAREMEVKVRSFAEQVEQRRRRLDMAVIFYTHEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZC0</accession>
    <name>T1GZC0_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02051735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02051737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZC0"/>
    <dbReference type="STRING" id="36166.T1GZC0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009219-RA">
        <property type="protein sequence ID" value="MESCA009219-PA"/>
        <property type="gene ID" value="MESCA009219"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860232_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046701">
        <property type="term" value="P:insecticide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046680">
        <property type="term" value="P:response to DDT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF45">
        <property type="entry name" value="CYTOCHROME P450 6G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009219-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15744" checksum="96BE0BC2EBE1DADE" modified="2013-10-16" version="1">MLPALLIFFVTELHIYKEYAQYPLRRPGNVIASIAFGLEANSFTNPQNEFRKAGLSVTDISLRRVVDAISIFFIPKLSPLVKAKLFPPIAENAFRRIFSYTIKNRRESGLFRNDLVDILIAFQDASKNGETEIEFTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-01-24" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZJ4</accession>
    <name>T1GZJ4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02378182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02378183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZJ4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009297-RA">
        <property type="protein sequence ID" value="MESCA009297-PA"/>
        <property type="gene ID" value="MESCA009297"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1909064_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="133" mass="14780" checksum="C886517889AA0845" modified="2013-10-16" version="1">MFLNLRPVRNWTWIISQQIVLLAVEELSSSLRLGCLYVNIEGNNIASQEDLSYSGRSDPDSDIIPMSPSNILRDVDTADPRWMDEYSMSSLLGHLDELANTSDTCNTPARSNDSSSLSIISETSVDYVTKFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-07-24" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GZX8</accession>
    <name>T1GZX8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02089603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GZX8"/>
    <dbReference type="STRING" id="36166.T1GZX8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009439-RA">
        <property type="protein sequence ID" value="MESCA009439-PA"/>
        <property type="gene ID" value="MESCA009439"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2443358_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="4">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009439-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="90" mass="9821" checksum="3E63D00B501B225E" modified="2013-10-16" version="1">MQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEAQVGGEKDLARAKDLNKEVYNFLASACSKYGLGFWKPGSGIIHQIILENYAFPVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H038</accession>
    <name>T1H038_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02193953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02193954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H038"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009504-RA">
        <property type="protein sequence ID" value="MESCA009504-PA"/>
        <property type="gene ID" value="MESCA009504"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2515240_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009504-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="85" mass="9049" checksum="F448FFB05D1DBCCF" modified="2013-10-16" version="1">MPPNIPTIPSWLRSAVACGRPNINPLVDRDAAVGYSFLPQSSSSVGHGDAELKPGTELSNTSKIKKKRSSFALYAAMVSCCVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H0D3</accession>
    <name>T1H0D3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl transferase family 25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02155202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02155203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H0D3"/>
    <dbReference type="STRING" id="36166.T1H0D3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009608-RA">
        <property type="protein sequence ID" value="MESCA009608-PA"/>
        <property type="gene ID" value="MESCA009608"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103394_0_0_1"/>
    <dbReference type="OMA" id="GEEHYIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050211">
        <property type="term" value="F:procollagen galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF53">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="4">
        <location>
            <begin position="36"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009608-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="190" mass="21768" checksum="DAEAF494C18F2C60" modified="2013-10-16" version="1">MGIKYLEGYKDPHENTTMTLGEPSQNMGANDKKGETQVLVLEDDIRFEPNFTSNALHILKQIRELPNWDFLYFGRMILSSQKEFTVHGIENIVSVDYSYWTIGYAITLEGAKKLVNAKPLKSLLPVDEYIPIMYDRHPNKTWSNVFPNRNLSAYSASPLLLYPQLYGGEPGFISDTEYSEIINTKDNCIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1H167</accession>
    <name>T1H167_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02173377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02173379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H167"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009921-RA">
        <property type="protein sequence ID" value="MESCA009921-PA"/>
        <property type="gene ID" value="MESCA009921"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2608780_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009921-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="79" mass="9257" checksum="0373FA130DEF03BE" modified="2013-10-16" version="1">MRFCIVVIYGEEMIEEIQAQETEEVHQSSDDHQVYNVSWPSDESAEINDVGLEDEDVKPDEDDFGTFQDFTEYDDLELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H177</accession>
    <name>T1H177_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02146708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H177"/>
    <dbReference type="STRING" id="36166.T1H177"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009931-RA">
        <property type="protein sequence ID" value="MESCA009931-PA"/>
        <property type="gene ID" value="MESCA009931"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313162_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009931-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="100" mass="11389" checksum="2E52FE69C846D51F" modified="2013-10-16" version="1">MIIFPLQSFYGHETDSLSSTTEGLKGTSPFLLHGQKSGTPTLKFHNHFPPDVQTDKKDHMIESGLGISEFGPPIKRSRMTPPLNERVMLYVRQENEDVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1C3</accession>
    <name>T1H1C3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02388908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1C3"/>
    <dbReference type="STRING" id="36166.T1H1C3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009982-RA">
        <property type="protein sequence ID" value="MESCA009982-PA"/>
        <property type="gene ID" value="MESCA009982"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_0_3_1"/>
    <dbReference type="OMA" id="QFGRMWW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041515">
        <property type="entry name" value="PPAF-2-like_Clip"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18322">
        <property type="entry name" value="CLIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588655" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="129"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009982-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="319" mass="34064" checksum="50DA6C5D68D38506" modified="2013-10-16" version="1">MDYFRILVCLQVLGIGFAFQFSSMDGSVQEVQDLQLDPNPTEDNEEVQFRQATITPTLVTCTCVPPGSCPVTPTLPTDGSGQIDIRIVNNPVTPTPTPAPCLYGLVRCCQPGAYKCGIRYPPPPNSKAALPGQAPFGAYPWQVAILSTVELYIGSGALLNSRHVLTAAHKVFNLTIASYKVRLGEWDAASISEPIPAEDVTVAAVFIHPNFNKDNLQNDVAVLRLSRDIALGQKSTIATACLPTAASYVGQRCFVAGWGKNDFGPTGTYQAIQKEVDVPVISNANCLTALRATRLGPNFILNDASFMCAGGEPGKDACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1C7</accession>
    <name>T1H1C7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">carboxylesterase</fullName>
            <ecNumber evidence="6">3.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CAQQ02007963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1C7"/>
    <dbReference type="STRING" id="36166.T1H1C7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA009986-RA">
        <property type="protein sequence ID" value="MESCA009986-PA"/>
        <property type="gene ID" value="MESCA009986"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2580680_0_0_1"/>
    <dbReference type="OMA" id="QQMALLW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA009986-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="81" mass="9064" checksum="BB0DF663DABD9B6B" modified="2013-10-16" version="1">MVWIHGGAYQVGEATRRLYSPDYFMKEDVVLVCLQYRLGALGFLSFKDKDLQIPGNAGLKDQVMGIKWVKENCAAFGGDPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1H2</accession>
    <name>T1H1H2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02112209">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02112210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1H2"/>
    <dbReference type="STRING" id="36166.T1H1H2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010033-RA">
        <property type="protein sequence ID" value="MESCA010033-PA"/>
        <property type="gene ID" value="MESCA010033"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884482_0_0_1"/>
    <dbReference type="OMA" id="VPRIEPK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF4">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="226"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010033-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="315" mass="36280" checksum="0AE62A146C2FDD24" modified="2013-10-16" version="1">VRGTIFNELDDEKIHKYIDFIDFEERFKVGIGGPIANGKDDADGSLQSFPSKRFKKMECVSLLENTRLRNIDVETKAYKDYIAERKDQNQLTEEDKFMLQLATRVDRTSSKLSIMNFMGNFFDSMHLICPQIYALMTASNSLKQSRKFKQVLEIVLAFGNYLNSSKRGPAYGFKLQSLDTLLDTKSTDKRQSLLHYIVATIRQKFPDILNFDSELYCTDKAAQVSLENIIADVHELEKGMEQVRKEADLRSKGSQIHILRDFLNNTEEKLKKTKVDLKMAQDAFKECVEYFGESSRNADASAFFSLIVRFSRAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1M3</accession>
    <name>T1H1M3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1M3"/>
    <dbReference type="STRING" id="36166.T1H1M3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010087-RA">
        <property type="protein sequence ID" value="MESCA010087-PA"/>
        <property type="gene ID" value="MESCA010087"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2819938_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010087-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="67" mass="7717" checksum="1F6685975EB7FAA8" modified="2013-10-16" version="1">TFKSFRLKKKSSTLGINKTSESPPSLRRDISPTNQHYGTYDPKLRHTTNGFDRNVPYRSSLQDMRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H1T1</accession>
    <name>T1H1T1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02158110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H1T1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010149-RA">
        <property type="protein sequence ID" value="MESCA010149-PA"/>
        <property type="gene ID" value="MESCA010149"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661609_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577821" evidence="1">
        <location>
            <begin position="25"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010149-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="76" mass="8698" checksum="487AC30F781FE939" modified="2013-10-16" version="1">MLTVKHRKIFVFLYIFQIFHHVSSWDGDGNGNAINVGLITDSNFDVLSRIFSKAIEDVNVYLETPLRPVVREISFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>T1H216</accession>
    <name>T1H216_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02121862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02121863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H216"/>
    <dbReference type="STRING" id="36166.T1H216"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010246-RA">
        <property type="protein sequence ID" value="MESCA010246-PA"/>
        <property type="gene ID" value="MESCA010246"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2838555_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="66" mass="7338" checksum="16D27186ADB5094A" modified="2013-10-16" version="1">MLTICNMYLFEMGVNTSQKVCKKVGKPALGFAETAEAVFANGPICLRNWSNVARNFVDCSLIATYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>T1H225</accession>
    <name>T1H225_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02376197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02376198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H225"/>
    <dbReference type="STRING" id="36166.T1H225"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010256-RA">
        <property type="protein sequence ID" value="MESCA010256-PA"/>
        <property type="gene ID" value="MESCA010256"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1043121_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="2"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="303" mass="34891" checksum="F35D11DD026168AA" modified="2013-10-16" version="1">MKQMILDFHNDVRNTIACGEPELTNIDGDIFPKAAKMPRLSWNSELQWTANLNAKTCAFHHDCAVTLSHPSAGQNIGMAVSKNATDTLNFLRNTIKGFFAEYLNTPLHVIDKYSPSVISNKFNRQLSNKIKDSELVEDPETLKENAHFAVLVQDKSDDVACSMYSCGKVEDFEYSYYLVCNYGYFNLLNEPVYKTSNISGSECNRNSGYYCCLCQNDDVEDDEILCHPSNINVPHFGKSNLSTNFMELHLYNFEKGANDFKFTPPKYESIQKYFEIYNTFVSREYIEMFSFLQFSLRKWNKHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2L9</accession>
    <name>T1H2L9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FGFR1 oncogene partner 2 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIKE family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02166508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2L9"/>
    <dbReference type="STRING" id="36166.T1H2L9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010469-RA">
        <property type="protein sequence ID" value="MESCA010469-PA"/>
        <property type="gene ID" value="MESCA010469"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922336_0_0_1"/>
    <dbReference type="OMA" id="NEMAGVM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008555">
        <property type="entry name" value="SIKE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186:SF2">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186">
        <property type="entry name" value="SIKE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05769">
        <property type="entry name" value="SIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010469-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15636" checksum="A1FA232276A5A25D" modified="2013-10-16" version="1">MIARLNLQNSNIREVRQENRELKACLEDHQYALEAIMHKYREHIQHKVLNSQINFKEQYNEHLLNIIRDQKEKINEMAGVMQRAAQMDEQPYYKELEMLTQLKLENKGLREMLQISKQFGSLNVGEPQAAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Q9</accession>
    <name>T1H2Q9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium-dependent multivitamin transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02195186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2Q9"/>
    <dbReference type="STRING" id="36166.T1H2Q9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010513-RA">
        <property type="protein sequence ID" value="MESCA010513-PA"/>
        <property type="gene ID" value="MESCA010513"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_7_0_1"/>
    <dbReference type="OMA" id="CTGSKQK"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF41">
        <property type="entry name" value="GH19970P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010513-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22234" checksum="506CAB0D0E802BC2" modified="2013-10-16" version="1">MFMPEISRNYFHVADYVVMSLMILGSAAVGIYFAFKKSSGHTHGQASTSAKKAITFGSADLDEYLLGSRKMHIFPVAMSLVASYVSAVTVIGTPTDIYYYGSQYWMIIISVILMGFVVSYVYLPVFQTLRLNSSYQYLELRFNFILRSMASFMFVMDEVLFLPMVVFVPSITFNQVTGFSAITISAILVCVCVFYTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2V2</accession>
    <name>T1H2V2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FDX-ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02145449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H2V2"/>
    <dbReference type="STRING" id="36166.T1H2V2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010558-RA">
        <property type="protein sequence ID" value="MESCA010558-PA"/>
        <property type="gene ID" value="MESCA010558"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987160_0_0_1"/>
    <dbReference type="OMA" id="NESTFHE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FDX-ACB" evidence="7">
        <location>
            <begin position="72"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010558-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="126" mass="14324" checksum="FCE9CBCC2856A73F" modified="2013-10-16" version="1">MEVLGCGIMRNEILIHSGVSNSIGYAFGLGLERLAMILFDIPDIRLFWSNDSGFLNQFNEDEHRINKFKAISTFPQCTNDLSFWLPDSMEIENFSPNDFYDVARGIGGDMIEQITLVDKFKHPKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H2Z2</accession>
    <name>T1H2Z2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02194745">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02194746">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H2Z2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010599-RA">
        <property type="protein sequence ID" value="MESCA010599-PA"/>
        <property type="gene ID" value="MESCA010599"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035254_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF2">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="103"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010599-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="310" mass="35528" checksum="F43A4422BD20D474" modified="2013-10-16" version="1">IPLDRGENIVLAYCEKLTKAPEIPLAKVCLQSLWRLFNNLCVTSPLRYHVYYHLVQVAKQCDQVKDVFTGVAQLKQQFSECPPSPEQLQKLYRLLHDVLKDTNSEMAAKVMIELLGTYTTENASVAREDAMKCIVSALADPNTFLLDPLLALKPVHFLEGELIHDLLSIFVSEKLPAYLTFYENHKEFVNTQGLNHEQNIRKMRLLTFMQLAESNPEMTFVQLTKELQIEESEVEPFIIEILKTKLVRARLDQANRKVYISSTMHRTFGKQQWXXXXXXXXXXXXXXXXXXXXXXXXXGPIERLVAIVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3L3</accession>
    <name>T1H3L3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02092436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02092438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3L3"/>
    <dbReference type="STRING" id="36166.T1H3L3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010842-RA">
        <property type="protein sequence ID" value="MESCA010842-PA"/>
        <property type="gene ID" value="MESCA010842"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1032775_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000060">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="16">
        <location>
            <begin position="63"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010842-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="30369" checksum="70863C9BC68B1A8A" modified="2013-10-16" version="1">DPIELENQFSIGNSLWFTIGALLQQGSEVAPKAPSTRTVASAWWFFQLIIVASWGGGQKEDPKLFSFMDPFSTDVWILLGVSYVTLENQFSIGNSLWFTIGALLQQGSEVAPNPINDVEDLANNKGDVQYGAKASGSTRNFFAKADYDTYKKMNEYMEKNPDLLVPQNGDGVQKVLNENYAFLMESTSIEYQLARLCNLTQVGGLLDEKGYGIAMRKSKIYNKVHHFLKKVIPDWPYRSKFNEAIIDFQESGVLTKMKNKWWNEVGTSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3L9</accession>
    <name>T1H3L9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02377528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3L9"/>
    <dbReference type="STRING" id="36166.T1H3L9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010848-RA">
        <property type="protein sequence ID" value="MESCA010848-PA"/>
        <property type="gene ID" value="MESCA010848"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1257331_0_0_1"/>
    <dbReference type="OMA" id="NMISTAM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010848-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="220" mass="23565" checksum="34A037DF10694909" modified="2013-10-16" version="1">MFVDRGNQELFYKPSQISSGNQTAVILCSSGSTGPPKAVTISHNVMKTIGSNFSGTCEEVSLSFTSLFWISGLNNMISTAMTGATRLVSCEPFNAELCLELIDKYKATKATFLPMHLAAINASPARFTKSMKSLNIIFSSGAPLPCETLNNFKEILSPNCRIIQGFACTEKGAITMDLYGKADGSCGTVAPNVEVKIIDNNGHSLGPNENGEIVARNFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1H3W4</accession>
    <name>T1H3W4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02194602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H3W4"/>
    <dbReference type="STRING" id="36166.T1H3W4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA010959-RA">
        <property type="protein sequence ID" value="MESCA010959-PA"/>
        <property type="gene ID" value="MESCA010959"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690668_0_0_1"/>
    <dbReference type="OMA" id="PEYKNHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA010959-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="74" mass="8792" checksum="9BD063805E8B4DCB" modified="2013-10-16" version="1">MNSIEEIFNNITYTNNVQSYSKIYKDRPMSSRDTAVFWIEYVIRHNGAVHMQSPLVHMNAFTQYSLDFILKKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1H498</accession>
    <name>T1H498_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02109688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H498"/>
    <dbReference type="STRING" id="36166.T1H498"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011104-RA">
        <property type="protein sequence ID" value="MESCA011104-PA"/>
        <property type="gene ID" value="MESCA011104"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645106_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011104-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="77" mass="8564" checksum="CB0052F27CCCA33D" modified="2013-10-16" version="1">MPVGPCFGETLSSTLPLPGGDDELYNHSGNGDISPNCHHPADISPTFDKPYMREMEKTIEASRFVAQHVRNKDKYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4Q1</accession>
    <name>T1H4Q1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02381004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02381005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4Q1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011267-RA">
        <property type="protein sequence ID" value="MESCA011267-PA"/>
        <property type="gene ID" value="MESCA011267"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2199950_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011267-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="108" mass="12212" checksum="47C35B8707B37331" modified="2013-10-16" version="1">MIANDLSHMPGVFALNPEFAHSSMGDSFYKCREKWELPLLLLPLTSWVLFPQTPNIEAHSIVSAQIILLTLVCACSEPFWANRATRKMSSHKNTHHFLTRFSPSTVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4Q6</accession>
    <name>T1H4Q6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02373120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4Q6"/>
    <dbReference type="STRING" id="36166.T1H4Q6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011273-RA">
        <property type="protein sequence ID" value="MESCA011273-PA"/>
        <property type="gene ID" value="MESCA011273"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163581_0_0_1"/>
    <dbReference type="OMA" id="NQIMHAG"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577993" description="Ion transport domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011273-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="93" mass="10794" checksum="C08742642A78A937" modified="2013-10-16" version="1">MLLLLVANLIILPVAISFFNDDLSTRWIAFNCLSDTIFLIDIVVNFRTGIMQQDNAEQVILDPKLIAKHYLKTWFFLDLISSIPLDYIFLIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H4V7</accession>
    <name>T1H4V7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02195947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1H4V7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011325-RA">
        <property type="protein sequence ID" value="MESCA011325-PA"/>
        <property type="gene ID" value="MESCA011325"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392231_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011325-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="94" mass="10817" checksum="CA1C5D6C40BEF189" modified="2013-10-16" version="1">MDKFESILNAFSNGYNPACTAYLELLISHLQKKENSNILQAPYVLKWVLSCLQKLEVDFSCFEPNILAFIIKLSALLTSNEWLVVHLRDEKLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1H529</accession>
    <name>T1H529_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02000494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02000496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1H529"/>
    <dbReference type="EnsemblMetazoa" id="MESCA011403-RA">
        <property type="protein sequence ID" value="MESCA011403-PA"/>
        <property type="gene ID" value="MESCA011403"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073231_0_1_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF131">
        <property type="entry name" value="MIP07328P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sema" evidence="3">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA011403-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="245" mass="28243" checksum="356F8196BC581759" modified="2013-10-16" version="1">FYKILSKSDDIVVGARNQLFSIRPNFTSHSLYSWSSTDADRELCTLKGKTAIQCQNYLRVFVKDYADKKNLICGTNSYKPRCRYVDFNAESEEQKVKEVEALGISPYDPIQNYTYIHADGQLYSATVADFSGSDALIFREPLRTEQYDLNRLNQPNFVASVDFDPYVLFFFREISATSVLSRVARMDEFLEEPTELFSAXXXXXXXXXXXXXKYTFSARWTSFLKSRLNCSVPGDFPFYFDEIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6J4</accession>
    <name>T1H6J4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6J4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012327-RA">
        <property type="protein sequence ID" value="MESCA012327-PA"/>
        <property type="gene ID" value="MESCA012327"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3362324_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012327-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="36" mass="3809" checksum="E9EF35024933E9F8" modified="2013-10-16" version="1">CSVNAVLALLGLDPPPFPLPSEEPLESFFESVSLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6K4</accession>
    <name>T1H6K4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="36166.T1H6K4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012337-RA">
        <property type="protein sequence ID" value="MESCA012337-PA"/>
        <property type="gene ID" value="MESCA012337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_593948_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="461" mass="51679" checksum="6FAD8A65337B6E44" modified="2013-10-16" version="1">SCLRQRVNSTTIIKFLDKLGGYLPIGAIGPSNLSSLVGKIYEIGPTPLFDIYYDLSSNRRRQVLLIISGPSTSAPILESKLRWMGPRNAPFYKIKDLVPKLLSELLDSFLPSGLSYDQKRSEIESITTFIAEFNKIRIENTRKGFWDSSLMSNISVLQEHHPFLNWSKLIPCNWTGQIVVRNPTYLHALQELLIKYPTRLAHNSILLMFACGVLPPLTPSPVICTRATMWALPEISASLYVAQHTGVVFEALKAYLKRAPSLKGAALVKLSQLKLQSKLWPGFHNLTAMITKLESYEISSEIILKFTRKVKVYRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAMRCVPNSVNVFSNYSNSRMDILIHSGGMQISYHSMLSLSPLKTMARLPGLNLTPTQVFFVVAAQELCAESDYTGINTELEDFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6V2</accession>
    <name>T1H6V2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6V2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012434-RA">
        <property type="protein sequence ID" value="MESCA012434-PA"/>
        <property type="gene ID" value="MESCA012434"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2419354_0_0_1"/>
    <dbReference type="OMA" id="HEEGHIF"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="92" mass="11441" checksum="6E7F3C62FC1407C8" modified="2013-10-16" version="1">QKTKPKKIKIHVPVKEKHHKHVHTVVKHIHHHHKPMLFKEEKVVKHEIHPIVQRKTVTHEHYHHHHQHQHPDEEEQIEEIEEHHHHHHEVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1H6Z7</accession>
    <name>T1H6Z7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="T1H6Z7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012479-RA">
        <property type="protein sequence ID" value="MESCA012479-PA"/>
        <property type="gene ID" value="MESCA012479"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3020312_0_0_1"/>
    <dbReference type="OMA" id="KYPRVHW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012479-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="56" mass="6500" checksum="D2D356BC83406506" modified="2013-10-16" version="1">KMHSPLESVGFISLFIVQIIFLVLYWVYVRYDVEALPKDETAEGNGLKEEHVSKYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1H7I4</accession>
    <name>T1H7I4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L39</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="T1H7I4"/>
    <dbReference type="STRING" id="36166.T1H7I4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA012721-RA">
        <property type="protein sequence ID" value="MESCA012721-PA"/>
        <property type="gene ID" value="MESCA012721"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181948_1_2_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA012721-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="39" mass="4809" checksum="88FFE4070DD7C262" modified="2013-10-16" version="1">KLAKKLKQNISVPCVRLRTKNTIRYNAKRNHWRRTKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GA89</accession>
    <name>T1GA89_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02003501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GA89"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000139-RA">
        <property type="protein sequence ID" value="MESCA000139-PA"/>
        <property type="gene ID" value="MESCA000139"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533983_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000139-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="84" mass="9275" checksum="8E7AE4E3E739904D" modified="2013-10-16" version="1">MDEPMIFSDDEEVSGTGTTTVTTTTVTTTTTEEHEEPKVKKRAYAGLPVDESTDAWMDNMDEPMVFSDDEEESKTVTTTVTTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GB54</accession>
    <name>T1GB54_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Menorin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the menorin family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02031831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GB54"/>
    <dbReference type="STRING" id="36166.T1GB54"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000475-RA">
        <property type="protein sequence ID" value="MESCA000475-PA"/>
        <property type="gene ID" value="MESCA000475"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033162_0_0_1"/>
    <dbReference type="OMA" id="GFTLWWA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019356">
        <property type="entry name" value="Menorin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184:SF6">
        <property type="entry name" value="CONSERVED PLASMA MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184">
        <property type="entry name" value="MENORIN (DENDRITIC BRANCHING PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10223">
        <property type="entry name" value="Menorin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Menorin-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000475-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="248" mass="28039" checksum="D36FDC9D84F4B5A8" modified="2013-10-16" version="1">MDSTSTQSNLTKIKWAHAVNSCTELQEALKNNKIQMLEADIILGQINNCGSFLPVMGHPPATSSDITLKDFLLTVLEFNQNAVNSKGVKLDFKSIEVFEKSLGILEELWNKITFEIWLNADIVKGPVEQTKPSQAVLSIGWTTLWGPEFREGVYTKDELKDMIAAIKDNHITNKNITFPIRAGIAANSLKEMKQFIQDLGKDNFYTFTIWSSVQDFVDVANLKTFILEFGFDRVYLDVPKDLEDKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBF0</accession>
    <name>T1GBF0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02068772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02068775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBF0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000582-RA">
        <property type="protein sequence ID" value="MESCA000582-PA"/>
        <property type="gene ID" value="MESCA000582"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1973011_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000582-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="127" mass="14593" checksum="F21FD1886E0D418B" modified="2013-10-16" version="1">MPEQLAEVQISAIRKFDVILEDQLYKIVPFADQVMNLRNFLEGAGCIEHLKSPSLLKELEAKLPYRERITWGGYSMSLRPPTVVEFSDWLLILADQIHRIQPKDGIQSMKKGDISHHAKTKTFAFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBH6</accession>
    <name>T1GBH6_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the actin-binding proteins ADF family. GMF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02393106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBH6"/>
    <dbReference type="STRING" id="36166.T1GBH6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000608-RA">
        <property type="protein sequence ID" value="MESCA000608-PA"/>
        <property type="gene ID" value="MESCA000608"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087056_1_0_1"/>
    <dbReference type="OMA" id="EWKMLYA"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11283">
        <property type="entry name" value="ADF_GMF-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000026">
        <property type="entry name" value="Glia maturation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF2">
        <property type="entry name" value="GLIA MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001788">
        <property type="entry name" value="GMF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="7">
        <location>
            <begin position="6"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000608-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="139" mass="16753" checksum="3BEB4E6F82E42845" modified="2013-10-16" version="1">METRNEICDISLDVKEELKKFRFRKSSTNSALILKIDREKQIVILDEFLDDISVEELQETLPGHQPRYVIYTYKMLHDDGRISYPMCFIYYTPRDSQMELQMLYACTKNALQRETDLTRVYEIRELDDLTEDWLKEKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBP7</accession>
    <name>T1GBP7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-lactamase-like protein 2 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02182703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBP7"/>
    <dbReference type="STRING" id="36166.T1GBP7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000684-RA">
        <property type="protein sequence ID" value="MESCA000684-PA"/>
        <property type="gene ID" value="MESCA000684"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_1_2_1"/>
    <dbReference type="OMA" id="GDHVMAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07722">
        <property type="entry name" value="LACTB2-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000328">
        <property type="entry name" value="Lactamase beta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000017">
        <property type="entry name" value="Lactamase beta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047921">
        <property type="entry name" value="LACTB2-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041516">
        <property type="entry name" value="LACTB2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF0">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17778">
        <property type="entry name" value="WHD_BLACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00069358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000684-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="270" mass="30738" checksum="166085245E96955C" modified="2013-10-16" version="1">MTLQGTNTYLIGSGKERILIDTGDEDVPEYINSLQKVLKDENAGIRDILLTHWHHDHVGGVKDVLKINPNQNCKVWKFPRYSHEPQDCPEIPENIKIYPLIDGQEFSVDGASMKVLYTPGHTSDHVCLLMEGIIFSGDCILGEGTAVFEDLFEYMKSLEHLLKIDASKIYPAHGNIIPEAKQKIEFYIAHRKQREEDILKVFNANPSQKYSAMDLVKIIYKETPENLWLAAAHNVTLVLKKLEKENKLVVCKGNEDEGPFWKLSGEHSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GBU4</accession>
    <name>T1GBU4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02394735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GBU4"/>
    <dbReference type="STRING" id="36166.T1GBU4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000733-RA">
        <property type="protein sequence ID" value="MESCA000733-PA"/>
        <property type="gene ID" value="MESCA000733"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801292_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000733-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16577" checksum="FBC28610950714A6" modified="2013-10-16" version="1">MAYAINGYRYHTKFDHIDYISRDSIQHTGNNVLELVKNLAHSLDLPNATEMTDKSMVFFDVFGFFFVSYSEDFATIFNYAVVIASIILPYLLLSRATRGINKKHLRFELFLGFLINMISLVGANAICYAIAYDLDHYGKSMSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>T1GC26</accession>
    <name>T1GC26_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02187740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GC26"/>
    <dbReference type="STRING" id="36166.T1GC26"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000822-RA">
        <property type="protein sequence ID" value="MESCA000822-PA"/>
        <property type="gene ID" value="MESCA000822"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123895_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000822-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="114" mass="12926" checksum="38D8D3602BEF56E4" modified="2013-10-16" version="1">MDNIPRTHEDIEAQIRDIQTKKKEIQKTQSDQGVSLLDKGGFFDTDLYDDGAGKDKYEGYNTSIATNDEMEEDEDEGLPVPQKRTSYTAPKSVMKEVAKIPSLWKVGEEKEGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCA8</accession>
    <name>T1GCA8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF753 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02061013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCA8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA000918-RA">
        <property type="protein sequence ID" value="MESCA000918-PA"/>
        <property type="gene ID" value="MESCA000918"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_809603_0_0_1"/>
    <dbReference type="OMA" id="CYSCEQD"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008472">
        <property type="entry name" value="DUF753"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721">
        <property type="entry name" value="GH09876P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05444">
        <property type="entry name" value="DUF753"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF753" evidence="1">
        <location>
            <begin position="154"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="DUF753" evidence="1">
        <location>
            <begin position="233"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA000918-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="343" mass="38195" checksum="C106A9781EC10FEE" modified="2013-10-16" version="1">MEKCVLCDSQKNPSCVANTNSSKTVDCKTACFSFINNVGDTVRGCAINDDICQNNELCSTCKTDLCNQEVFPESRLQCVQCENCFNSTTLPQMSYCDIYNPEDECYTYFDPKTLLTIRGCTSSSMPPMCGNCNLEKCQTDGCNNKQPEISNFFCYECYGSDEDCVSGKNLEAVRCAHGDRCITQYDNSTGNLWRSCVASKTEECSKEDEANGFCEICTKDKCNDQLMPSDRLYCLQCEGQNATNCYNGTVGSTPCLRAQKFDRCYSYIDHKDVMHRGCESDGFCDGSDQCVYCGKDGCNNVDGNGNPDLPNVGTFLVSLVMEMMLHVYWDQFQAILVSTETNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-11-27" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>T1GCL7</accession>
    <name>T1GCL7_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CTF/NF-I domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02146701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02146703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GCL7"/>
    <dbReference type="STRING" id="36166.T1GCL7"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001033-RA">
        <property type="protein sequence ID" value="MESCA001033-PA"/>
        <property type="gene ID" value="MESCA001033"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163621_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000647">
        <property type="entry name" value="CTF/NFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020604">
        <property type="entry name" value="CTF/NFI_DNA-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492">
        <property type="entry name" value="NUCLEAR FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492:SF8">
        <property type="entry name" value="NUCLEAR FACTOR I, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51080">
        <property type="entry name" value="CTF_NFI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTF/NF-I" evidence="5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001033-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="239" mass="26927" checksum="1A21EED10709F025" modified="2013-10-16" version="1">MSLEEERVCKEELQNEKGETKQKWASRLLGKLRKDITQECREDFVQSITGKRQSICVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKAIPLESTDGERLEKNPDCSHPGLCVNPYHINVSVRELDLNNNNNVETKKEIERRKGYTHNPYNGVVCNDIILATGVFSSKELWRLSKGSILDEPSELIQTNPNTIKMENTGGAYYCNSYPLSTAPPGIVSAVSDRSLGSSMVVSSGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GDK2</accession>
    <name>T1GDK2_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02148396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GDK2"/>
    <dbReference type="STRING" id="36166.T1GDK2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001391-RA">
        <property type="protein sequence ID" value="MESCA001391-PA"/>
        <property type="gene ID" value="MESCA001391"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_317912_0_0_1"/>
    <dbReference type="OMA" id="WLEPITT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043537">
        <property type="entry name" value="Tiam1/Tiam2/Sif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46001:SF3">
        <property type="entry name" value="PROTEIN STILL LIFE, ISOFORM SIF TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46001">
        <property type="entry name" value="TIAM (MAMMALIAN TUMOR INVASION AND METASTASIS FACTOR) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001391-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="916" mass="103124" checksum="7AE02C4B7772E2D9" modified="2013-10-16" version="1">MDHLLTHIKYEGYTAKNGFIERYFEATNKEQTRSKQQKTNKSNTNLNFLTPNLHPKEHILAALQNSESSDNNNSITTTDDNSKKISLHCCCVIVDITPTTQSFKDVRGSNSNQYGGGGGGGSNRSLASSLFKFGSSKRPVCICNARYQSLAEGGIGPSIKTHHGSLDRLLQQQQKQQQQQQQQFPRHAPEVGEVKGFVKGSGENECSNKGIESPSYRPPSSPPPPIPASRHLSKRLSQSSHRLDQMKEEFKKGVEVEKFYHQINGDIFEITRRIRAKEKPPEEERKSSLKAPQPPPRTSSRQAKYAADSFEVPPSYVNGLIESVNKDIRVESGTSRDCVPKITTSQRTSSGPSELTVLKDDIPEWLIEHLQKKNPVERQDSSKSTSSAAQALDKAKKLKDELTELLKKPLQRQISGSSVRTDISEWLTKFQMEYKPKPAIRSSIHKDEPHYKEPKKRHSLGHTEIKAENIPEWLIIQLQEQQKIQASRKLSIAAQGSYLDLQKASTPHREKHDRCKERKVRHSVSEITPALPPAAQLQAQKLANIEKLYAKPHKERKEKYQYVPVPKECKGSQHKSRYQRSATVTNIFENTCKYTKDKRKSSSAERAPRSPTPTTICTDPACGQMPICTDPNCCYDPRISCSYMNSICIGPQGPPIECTDPKCHGLSSLKCNSLPRSAADLSRSQFQPTTFFTYQRSNSQPNTLTREESNQVALAIAPPIEKPKTKKNGHTRHKSVCEVGNGSSNGGSKLMKSASAASLNSRRRRHKTVHFGENLMREVCQNRRFIKQTPDTPSGSAPLQPNIQLLFNFVEGVLSAWVDDEEDDRGDRIGAESDPENNNIHNKLLPIHRCNRYRFQAIHRICKEAGMLKGTIALGNVRYRHHHWKSTTEICNERFLRKLPSSAKVHEFIDGLDQTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE05</accession>
    <name>T1GE05_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02159858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GE05"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001560-RA">
        <property type="protein sequence ID" value="MESCA001560-PA"/>
        <property type="gene ID" value="MESCA001560"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1130190_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF70">
        <property type="entry name" value="JIL-1 ANCHORING AND STABILIZING PROTEIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="12"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001560-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="246" mass="28394" checksum="61AD3702C82B558E" modified="2013-10-16" version="1">MATPKKSKVFELNQLVFAKVKGYPHWPARITEVKPTKTCGVLFFGTNEVANVKSENLFNYYDYKDKFINEKSLKNPDFLKATVEIRTQEEDSEGAEKVNKNLDSGKKSSPKQADKVVQLTEKRKSFSPKSKEIVITIKKRRLTPEVGNIENNNKSVDDLNAVNKDAVTFCSKSAQLYRILERVDENTSFQACAEILDELFKIDYSKDLLLSYPDEFCNIYYLQKLKVFHPLMKRSAFEXVTNIEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE42</accession>
    <name>T1GE42_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (32-2'-O)-methyltransferase regulator THADA</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02042870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02042874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE42"/>
    <dbReference type="STRING" id="36166.T1GE42"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001600-RA">
        <property type="protein sequence ID" value="MESCA001600-PA"/>
        <property type="gene ID" value="MESCA001600"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_266604_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF7">
        <property type="entry name" value="THYROID ADENOMA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUF2428" evidence="4">
        <location>
            <begin position="479"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="5">
        <location>
            <begin position="736"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="1242" mass="141515" checksum="26CC4D60394A5AE9" modified="2013-10-16" version="1">MQESDFCSYTKEMSKIINNIVNIGIESCSIPNILLASARNLYNFSKSLLSFKSLKENETVVKSMLSQMNSYALAFFEHHMDSVRHLCKDIFKNVVVLAKILDFNKLLQDILKSCKEEFALSISSVVLLQMSQALGTTYILENMEGIFEKYFDKNLGSDLHVNNFFEGLMLTHHKEVDISNWQDLWIAYLLNSANSSTNQRLAEIENLVSKAVKCEPQVLNFICEYKLSKISISTKLAALSSAKKSSNIQPFSADFREAILSSSDDTKILALKLLIESRKTTEIPATRQKSLALLEKSLTRLSLNIEKLQKTKVFDLESNQFYLKFLQDFTEILTGNLDDGSNFSRRSISLNLLEKCVEIFKNFHLPLEKLLAEKLENALFSRLEDSYEKNVHKLIVSVKPTDSLTAAYQCEYICESSESSYLEILNWVKNILMDGLVIAKKSISLASRDNPLYGALVVIKHLLDKLDLNKITDNSWKAFINEMIGLSKELTRVVGPIVNNSSPEGHLPNDFSPLDLGDDENKEYLESLNTKRQKTHNVKITPQMVLICAWRTVKEVSLILGNIVLYSPLSILITKDQVIEIGEHFKELLSETKHRGAFEQAYVGFSKLCTRLWRESELHQLPMIWLTNLMEEISKDENVSSKMCATRRSAGVPFLVQALITSELQVGTTKALNYCMNTLISICYDSSKSTESRVHALNILRALFRCTDLNEAVSSFITDGMICSIEGYDANTWAERNSSTLTYILPAYPRLYDFILAQLEEASKGVYEENFGGLTQISTRKLHPLLLLLSRLYPSALEGTESNLKLSDFIPYISICSSCKELQTRILAAKAISAIISKDHVEEYLSSICSVISSEFSLPTNFVHGQILQILHLIRGNQISREIEKEIFESLIKVQKEIDNAVILTCIFDIFNEILRKTLSGSNTIPLDKIKSDYQQKESFYYPVLRKSLMIFKIHKLRLENNFEVIGNVLCNFENASYEEIETKLNIILLTMTECPDEQFEIRNDEISCVKILEEKPSLNVENLLDQVLSSNIFYPECAIKAYAFVFSTDWTSKTPLAMCVEKYTRTNNKFETFLKCIEFLKDVSQPWNTDCLRFRAACILENICSNFIIAVNLRNWEALIDSTLILLNLLQDDELYVRQQAALVVLKCTGKSEDCDVLPLYAAEIFISWLAMQLKVLNSNEDIQRVFQEIVNIHGSNECVRGNDCDDIEIFDKNEANVFAEPRVIVDLVTKVFRDQFDFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GE96</accession>
    <name>T1GE96_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02033144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GE96"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001654-RA">
        <property type="protein sequence ID" value="MESCA001654-PA"/>
        <property type="gene ID" value="MESCA001654"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2545219_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001654-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="83" mass="9474" checksum="ABDFA686AC371765" modified="2013-10-16" version="1">MRATKEAFQNKRRGVVKLARPVEKVLRGRKPSAEKIFNNFFLRQMTLQDTSSCVKTAEGVSEKFQTLRGFQQGDALSCSFFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEA1</accession>
    <name>T1GEA1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02175216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEA1"/>
    <dbReference type="STRING" id="36166.T1GEA1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001659-RA">
        <property type="protein sequence ID" value="MESCA001659-PA"/>
        <property type="gene ID" value="MESCA001659"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248038_0_0_1"/>
    <dbReference type="OMA" id="FMRHIRS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="67"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="183" mass="21508" checksum="FE0D1A55190D135C" modified="2013-10-16" version="1">MLKQIQAKGVVNVFGFLLHIRNQRNFLVQTEEQYIFIHDALVEAIMSGETNLRVEQIQELKKNTTYLEQLYKNIIQFQAKDIHISSAMKQVNSIKNRGAIFPVDSYRVHLTPKPGEEGSDYINATWLHGFRKLKDFIVTQHPMNHTVKDFWQMIWDHNVQTIVLLSSLDEIVSIDFYRKFNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEN3</accession>
    <name>T1GEN3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02071796">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02071797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEN3"/>
    <dbReference type="STRING" id="36166.T1GEN3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA001801-RA">
        <property type="protein sequence ID" value="MESCA001801-PA"/>
        <property type="gene ID" value="MESCA001801"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_473498_0_0_1"/>
    <dbReference type="OMA" id="VYPMEHS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050663">
        <property type="entry name" value="Ankyrin-SOCS_Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193:SF128">
        <property type="entry name" value="GA-BINDING PROTEIN SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="507"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001801-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="586" mass="65360" checksum="522BDC1A282B413A" modified="2013-10-16" version="1">MTSFPLKPPLNNKNIIATIASDGQVYSLDNNTSNFVKMLPFASLNGTDGDHGESMLKTTPAAILDLGKNLIQYAKDDNVEGVAEMMAKGAPFSSDWLGMTPIHYAVVNNSIPITEVLLNAGVNVDVKTKVDRTPLHLAAYYGRDEIAEMILSSGKCRMLLRFDADPTVVSKFGKTPIAIAVQTEQPDILEILENAKNSQQSKKFNEESMANAQLMDETDKEASDAVNSILGLTGEPAPPIEVEDDEDNKVRAIICNHDDDDDQSTDVDSMTYLTNSMNGHGNQTTLEMLKTHGISMISEDDEDSSSNLLTSALQSGRTLVLSEVNGDNKPKIVTNSKPAMHFINPKEKKNITTVGNKNVRVISMEELQKMNNDKFKPTMKRITKVAPQIQQTAPRKVFMSPKITQPHQINPITRKIEDFSEDEISSKIPKVTSGTFTTNLSELERRKPTLKVIKHTPTSSPSSLGAIKIQNSQQRFTPNQTTNIQPPQRTKGPVLPLLNTADTSREIFQLKKQVKEMKFRMDDVQRENEQLRRRIDKLEGLQLENESLTARLEHIEKILCNQIAMENPDDVIEEEQDFDESIMQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>T1GEW8</accession>
    <name>T1GEW8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L44</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02157785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GEW8"/>
    <dbReference type="STRING" id="36166.T1GEW8"/>
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        <property type="protein sequence ID" value="MESCA001891-PA"/>
        <property type="gene ID" value="MESCA001891"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114645_2_1_1"/>
    <dbReference type="OMA" id="CKKHTIH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA001891-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12514" checksum="3AC2323763CC089F" modified="2013-10-16" version="1">MVNVPKQRRTFCKKCKCHKLHKVTQYKKSKERHGAQGRRRYDRKQQGFGGQTKPIFRKKAKTTKKIVLRMECTECKYRKQTPLKRCKHFELGGDKKRKGQMIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFJ0</accession>
    <name>T1GFJ0_MEGSC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02070302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GFJ0"/>
    <dbReference type="STRING" id="36166.T1GFJ0"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002132-RA">
        <property type="protein sequence ID" value="MESCA002132-PA"/>
        <property type="gene ID" value="MESCA002132"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111623_1_0_1"/>
    <dbReference type="OMA" id="IMPTICE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002132-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="78" mass="9094" checksum="BEEC150A33C245D7" modified="2013-10-16" version="1">MPTISEDNISNRSSQFSGEEANFEQLMVSMLDERDKLMESLREAHDRIQENDSKLQETVKERDSLQRQLNANLPQQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFS7</accession>
    <name>T1GFS7_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA002227-RA">
        <property type="protein sequence ID" value="MESCA002227-PA"/>
        <property type="gene ID" value="MESCA002227"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1817996_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002227-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16044" checksum="069D06D4734E52FD" modified="2013-10-16" version="1">MRHPGWDMLIGGSAKGSMCWHDGLSKVNSNHVSRGVANFKCYGLVVESIAAKSALRLQSVGLFKERSYGHSAILSQVDIGKPNTDYIGQEYDFNILNSLHFEHFRSTRDSPIKMPCKSFNYRHIDDMSHNVRHCQSIMSVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GFZ0</accession>
    <name>T1GFZ0_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="MESCA002293-RA">
        <property type="protein sequence ID" value="MESCA002293-PA"/>
        <property type="gene ID" value="MESCA002293"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294836_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002293-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="101" mass="11760" checksum="DC68151FFB35139D" modified="2013-10-16" version="1">MRRFRQEWPLTNFLQKFHNNEGEIPLTRTEMDKAVQRPKNNKIAGTDGIPVPAQDHHARYASPSNFTFEGLENAVKSRELYVNGNSLVSTTRKRELLWHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GG31</accession>
    <name>T1GG31_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02198407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02198409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GG31"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002337-RA">
        <property type="protein sequence ID" value="MESCA002337-PA"/>
        <property type="gene ID" value="MESCA002337"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818017_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002337-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="142" mass="16423" checksum="C97367CDC312D5BA" modified="2013-10-16" version="1">MLHVSKLQKIVKQLQDLGEVISDNMLITKILMTLPENYNYFFTSINDGRRKNTEKPNHQIKHGGIKRCAQRPETWPGTRFLSAERQGWELPVIYYDDFVQNGDDLDDYSDEIACFGVGNSTENNINRIFVPKNPSKNSLLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGH5</accession>
    <name>T1GGH5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Malonate-semialdehyde dehydrogenase [acylating]</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Probable malonate and methylmalonate semialdehyde dehydrogenase involved in the catabolism of valine, thymine, and compounds catabolized by way of beta-alanine, including uracil and cytidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20804"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:20805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3-oxopropanoate + NAD(+) + CoA + H2O = hydrogencarbonate + acetyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76615"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:76616"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="EMBL" id="CAQQ02059024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGH5"/>
    <dbReference type="STRING" id="36166.T1GGH5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002495-RA">
        <property type="protein sequence ID" value="MESCA002495-PA"/>
        <property type="gene ID" value="MESCA002495"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_10_1"/>
    <dbReference type="OMA" id="GGAKNHI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="10">
        <location>
            <begin position="153"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002495-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="463" mass="50370" checksum="1BB252FDDC15C6E9" modified="2013-10-16" version="1">MFLQRFRKFSTAPTTKLFIDGKFVESKTTEWIDVHNPATDEVVTRVPKSTKAEMEDALESSKRAYKTWSQTSPLTRQQVMFKLQAIIRANMSEIAKGITKEQGKTLIDAEGDVLRGLQVVEHCCSIPSLMMGETVPNVAKDMDTYSHTLPLGPSERVPGATMEIMEMLNEAGCPPGVVNVIHGAHDAVNFICDEPAIKAVSFVGSDTAGKYIYERAGRNGKRVQSNMGAKNHGIIMADANKENTLNQLVGAAFGAAGQRCMALSTAILVGDAKNWLPELVERTKKLKVNAGHLPGTDIGPVISKQSKQRINDLIESAVKEGAEIVLDGRDISVSGYEKGYFVGPTIISNVGTSMKCYKEEIFGPVLVVLLADTIDEAIAITNSNPYGNGTAIFTTNGATARKFVNEIDVGQVGVNVPIPVPLPMFSFTGTRGSFLGDNHFYGKQGIKFYTQTKTVTQLWRQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGK8</accession>
    <name>T1GGK8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit</fullName>
            <shortName evidence="9">Oligosaccharyl transferase 48 kDa subunit</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02186817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGK8"/>
    <dbReference type="STRING" id="36166.T1GGK8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002530-RA">
        <property type="protein sequence ID" value="MESCA002530-PA"/>
        <property type="gene ID" value="MESCA002530"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031804_0_0_1"/>
    <dbReference type="OMA" id="AHDEYPR"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147038" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit" evidence="9">
        <location>
            <begin position="20"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="10">
        <location>
            <begin position="26"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="11">
        <location>
            <begin position="272"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002530-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="430" mass="48484" checksum="5D30A8F54EA4C71E" modified="2013-10-16" version="1">MDVKLIFLGFFLLFCGSQAVLENEAETLVLLDTLAIRETHSIFFKTLSDRGFKLTFKLADDPSLLLSKYGEYLYKNLIIFAPSVEEFGGDINVDKLSQFLDDGGNVLVTGNSDSGDALREFASECGFEFDEDGSSVIDHINYDSIVGKRESVNPMFYKGTSIIADKENPLILQILTGDSSSYSYNPSSQITSYPHGVGKGTILIAALQARNNARAVFSGSLYFFSDEAFTQSVQFAQHEKFFKNSGNQDVATALSKWVFGETGRLRVAEVNHHKEGETQPPSFYTIVEPVVYKIKIEELDGNTWKPFQADDIQLEFVRIDPFVRTTLKKVGTSGVYQANFKIPDVYGVYQFKVDYDRTGFTHLYSTTQVSVRPLEHTQYERFIPSAFPYYMSAFSMMIGVFVFSFVFLHMKDEPGTKEEKSKTKDDKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2024-05-29" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GGY8</accession>
    <name>T1GGY8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoporin Nup188 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02144438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02144439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GGY8"/>
    <dbReference type="STRING" id="36166.T1GGY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002669-RA">
        <property type="protein sequence ID" value="MESCA002669-PA"/>
        <property type="gene ID" value="MESCA002669"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1334821_0_0_1"/>
    <dbReference type="OMA" id="LILEVWH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002669-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="206" mass="23959" checksum="AF7762DCF15D63D9" modified="2013-10-16" version="1">PYYKYLWQLVSGIHYETPEEKVRTELSNVSKKICEGILQFRPACASKTDLETLLEGKHQEKLIPFTKKLQNLLNLETSQCWEILCSYLTHEFRGSASSLAVFVANETNTTKLLEDIWGFYSLERMIVLKIIKNMLLFYEDAAHPFHEQYVQCIDKITLTKLRDSYFKQFKYLLEDKPASSLTSVLVFIFNECLTSGVFPDCLMNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHP3</accession>
    <name>T1GHP3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02039603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02039606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHP3"/>
    <dbReference type="STRING" id="36166.T1GHP3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA002951-RA">
        <property type="protein sequence ID" value="MESCA002951-PA"/>
        <property type="gene ID" value="MESCA002951"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099695_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040034">
        <property type="term" value="P:regulation of development, heterochronic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000411">
        <property type="entry name" value="Ecdysone-induced protein 74EF isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF191">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 74EF ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="8">
        <location>
            <begin position="144"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA002951-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="240" mass="26428" checksum="746459DF8CA8FDAB" modified="2013-10-16" version="1">MPTGGGTSNGGSGGGGNGGGYGNSPHFQTPSPPRTNGAQPSSHHSIHHPHHPHNPHLSSHNHHSAVAAAVAMHHHHHSLNGGPVNANGGLHPGANSIIQAPTSSVDDLSYMLELGSFQSRKLKKPRKPKIEMGVKRKSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGAHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIVEIDCNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GHY8</accession>
    <name>T1GHY8_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRTCAP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02093951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GHY8"/>
    <dbReference type="STRING" id="36166.T1GHY8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003054-RA">
        <property type="protein sequence ID" value="MESCA003054-PA"/>
        <property type="gene ID" value="MESCA003054"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109648_2_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018614">
        <property type="entry name" value="KRTCAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32001">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32001:SF1">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09775">
        <property type="entry name" value="Keratin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003054-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="98" mass="10868" checksum="C365CCB8BFCE46F9" modified="2013-10-16" version="1">MRNSIQVFSLLISIICSLILIAALRVFNDFLSASGIGIFLCGFIGNWIFIFLLTCTSLGEMILFGDYFQAQLFPEVSLCLGSAMFVCCLVHRLSATTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIL3</accession>
    <name>T1GIL3_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02001167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIL3"/>
    <dbReference type="STRING" id="36166.T1GIL3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003294-RA">
        <property type="protein sequence ID" value="MESCA003294-PA"/>
        <property type="gene ID" value="MESCA003294"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860235_0_0_1"/>
    <dbReference type="OMA" id="MVAASQH"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000383">
        <property type="entry name" value="box A-binding factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052294">
        <property type="entry name" value="VSX_homeobox_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46892">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46892:SF3">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="89"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003294-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="138" mass="15435" checksum="8925F5DC28ED1137" modified="2013-10-16" version="1">QSRVEFPNLNGSGEESRVNASNNNNNDAVTAAMVAASQHHHPGFHPVSTTPGHPHHPQHPHHPHHPSHHHHHPSDGLSPPNSNKKKNKRRHGRTIFTSNQLEELEKAFKDAHYPDVSARELLSMKTGLAEDRIQYDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIS4</accession>
    <name>T1GIS4_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02199602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIS4"/>
    <dbReference type="STRING" id="36166.T1GIS4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003359-RA">
        <property type="protein sequence ID" value="MESCA003359-PA"/>
        <property type="gene ID" value="MESCA003359"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075666_2_2_1"/>
    <dbReference type="OMA" id="LAKHHTM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000043">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="58"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003359-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="156" mass="17310" checksum="76FA813095327DB7" modified="2013-10-16" version="1">LIFLAFIFVNWSEKEVKNGKIRKKSSIKKEKKSEASLIPQELPASGIKKVGKKPGGGSKKKKPLSYGIFLYRVLKQVHPDVKISSNAMSICNSFCNDIFERLASEASKLTKLAKHHTMSSREIQTAVRLLLPGELAKHAISEGTKAVTKYQASKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GIV1</accession>
    <name>T1GIV1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02140148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GIV1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003386-RA">
        <property type="protein sequence ID" value="MESCA003386-PA"/>
        <property type="gene ID" value="MESCA003386"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1422998_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004588322" evidence="2">
        <location>
            <begin position="19"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003386-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="191" mass="21021" checksum="792A92426A99FC1E" modified="2013-10-16" version="1">MKLAVLFVGLSLFLAVSARSADDAKALNLLPFIPITNEEYQSLRSLVGKKFTARGIFDKLSTESSRGLFDKFKGSKPLCTVDAGGVNLGTSSSGGGSDGEKGGIFGFFKKKKNKDDTPTSTIPSSSGGDFYDYNYDYESFPVAPPFKKGKGKQTTHKIQRRRPGQRQHVQRQSGDRPNKRQQQRRRTTTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GJL4</accession>
    <name>T1GJL4_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02073830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02073831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GJL4"/>
    <dbReference type="EnsemblMetazoa" id="MESCA003663-RA">
        <property type="protein sequence ID" value="MESCA003663-PA"/>
        <property type="gene ID" value="MESCA003663"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2673917_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA003663-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="75" mass="8897" checksum="94D0A6838D59C9E8" modified="2013-10-16" version="1">MKRQLITNSDKIGIQLEYVLLLEQTMFNILPKLREILKTFLSKQILGDFFGNSVIIVDRRNHLRRFVQCPTFKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>T1GKN1</accession>
    <name>T1GKN1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02055780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02055781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GKN1"/>
    <dbReference type="STRING" id="36166.T1GKN1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004058-RA">
        <property type="protein sequence ID" value="MESCA004058-PA"/>
        <property type="gene ID" value="MESCA004058"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_5_1_1"/>
    <dbReference type="OMA" id="ECILECV"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06799">
        <property type="entry name" value="PDZ_MPP3-MPP4-MPP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11862">
        <property type="entry name" value="SH3_MPP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
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        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="37"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="114"/>
            <end position="181"/>
        </location>
    </feature>
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        <location>
            <begin position="262"/>
            <end position="446"/>
        </location>
    </feature>
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        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004058-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="51761" checksum="397777969D3954D3" modified="2013-10-16" version="1">KLLISLHLCNIPATSQRQQIYQLPTKILIFSTKMFSGATIKTDEETGKIIIARIMHGGAADRSGLIHVGDEVMEVNGVDVEGKTPTDVLTILQNSEGTITFKLVPSDGKSGSRESKVRVKAHFDYNPETDPYIPCKEAGLAFNRGDVLHIVSQDDAYWWQARLIPSRALQERRIIHERTQKAQETDSKSTCTSICYTPPASPMLTSNNSNNTSHSNTNSTITCRGPKTKKIMYDLAENNDFDREMIATYEEVAKLYPRQGVYRPIVLIGAPGVGRNELKRRLMARDPEKFRNPVPYTTRPIRQGEVAGREYIFVTREKMEADIAAGKFIEHGEYKGHLYGTSGESVKAIVNAGSVCILSPHYQAIKALRTPQLKPFIVHIKPPSFEKLKESRTSARAKSTGFTDEEFEEMIKSAERIDFLYGHFFDDEIVNADLAKSFEILVSCVQRAEEEALWTPASWVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLC3</accession>
    <name>T1GLC3_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02012820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02012821">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLC3"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004323-RA">
        <property type="protein sequence ID" value="MESCA004323-PA"/>
        <property type="gene ID" value="MESCA004323"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870162_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004323-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="64" mass="7460" checksum="8CE3048D065A5E65" modified="2013-10-16" version="1">MEKIFSRISHYQKAISVYWQNNFSAMALYCVAVFPDSDTFASKIPFLEVLAYLFSSFPSIFPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLF9</accession>
    <name>T1GLF9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02022588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLF9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004360-RA">
        <property type="protein sequence ID" value="MESCA004360-PA"/>
        <property type="gene ID" value="MESCA004360"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1932822_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF8">
        <property type="entry name" value="LD22649P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004360-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14701" checksum="E4043942EBF4F803" modified="2013-10-16" version="1">TEQIRRANRLFASDSLFLRQYLLIPVEKSSPYYPKEDRPQSFPSMNFSLNDTPTSSSPASGSSELSLQNEEVVQEHNNRNTSCSTESMDTADNPTLSPEEESRKSINDFLDKIDNTIAASKQYVAKSQSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLN8</accession>
    <name>T1GLN8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02394080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLN8"/>
    <dbReference type="STRING" id="36166.T1GLN8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004450-RA">
        <property type="protein sequence ID" value="MESCA004450-PA"/>
        <property type="gene ID" value="MESCA004450"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827512_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004989">
        <property type="term" value="F:octopamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004450-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16525" checksum="B8A126D50FD40938" modified="2013-10-16" version="1">MKVCRRNFRSYLLKAFNIPFHIQSIELKIQKIENGNIEKYVTTTLCETCLFPDIMIAILFWIGYFNSTLNPLIYAYFNRDFREAFKATLACIFCSWKNGGLPLDIDMRRSSIRYNNRTRSIVSESYVNTNYAQCGNNTKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>T1GLV2</accession>
    <name>T1GLV2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02019004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GLV2"/>
    <dbReference type="STRING" id="36166.T1GLV2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA004516-RA">
        <property type="protein sequence ID" value="MESCA004516-PA"/>
        <property type="gene ID" value="MESCA004516"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751814_0_0_1"/>
    <dbReference type="OMA" id="ENDEHYL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47326:SF1">
        <property type="entry name" value="HTH PSQ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47326">
        <property type="entry name" value="TRANSPOSABLE ELEMENT TC3 TRANSPOSASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA004516-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17357" checksum="3BC6EB12FCB87E99" modified="2013-10-16" version="1">MDDSDDTLNKIFYTDECIFNQDGIFNYHNSHYYRVSRSQTKLSVNIWCGVLNGKVIGPDFFDENLTGDVVLNFIRDVLPTLILKARENPDDLILLLDGALPHYSSVVRRYLNEKYSLFWIGLEFILLVSKIHRSFTSRFLFVGRLKDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>T1GND1</accession>
    <name>T1GND1_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02030761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02030763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GND1"/>
    <dbReference type="STRING" id="36166.T1GND1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005078-RA">
        <property type="protein sequence ID" value="MESCA005078-PA"/>
        <property type="gene ID" value="MESCA005078"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_307633_0_0_1"/>
    <dbReference type="OMA" id="YENEHIS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005078-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="961" mass="113615" checksum="7DDF4C14ED10D2AD" modified="2013-10-16" version="1">MTKAVIKADNVHTPETMSSGSSICELIDLENPLKMDFHGVDYKETYKNDEQTFQNQQEYIRVKRQMNTHSIKANMEKVLGAEKSKQIRKVPSVLDVNYDYDSNEERITNPSLHGGDSTIRTPTPHHEETPRLAKLFRTEQKPFNISIIEMEEDFRKDRFLNLFQENEEREQEEKKLRKVTIQNEKENEVEKKSLKEEEKELVKCYLELKKVQKGKIYPHTLKESLQFKHFLKKQKIELKEKALIKQDFYKELGVVKKYKEAELDISSLSTSIEKISEMSTDDEDWFKVGQQTRDSSQYFLRGFHLKMAKEPRGKFHQKEKGVGGRIPRSCMTRTQWFDDMAPKSKKEKSDTQNVSLLKKERNPDDPNLRLFPIATGKAVIRDLSPVKEYVIQPLTEMGKRTSEMKIIHTKKPKENIKVKSFNMTGFMYGKDDIVQKSFDEDNKQEERTLKEYIKDKELLRKKRIIRKRKQRCHYLKIYLPQRRTKVVKEDFQNYTSSDSDWIPEKQNSIDSSYAERLSVTRIGGKFAIPIIHGIEELKYNMPAPNFRETTLQRNPRNPRDYENEHISDDEVFNYLKQSNKPLMLKIENDRKNAENVNKKKTIYGRIFDARRHHDHWDPTQHKVVDKKDHSDIKRISSMEENEKKRSRDPNEFLSEYYGRHEFLNLIKDISTELKAKRPKESGDIEGIVDRTEYLAEIENDLKSIKSCLSSLDSSINTNFTNDSGSFLMLESHKVPLDYFRKSVVPFNEFNRVRLKAKPIPPVQFRDPFEIVPFSGQKQEQYKLVKPKDKLHTFNTRIQSERTLRQDLKNQGDSKELLGYNRQVAIDVDELYIEDLSNGSQVKHFRPATMRSILKNSLRKRFEGWVIEQKVVEQKIAEKKEEMYANEIIKLKLLLNKLFIKWEKKEYDYSMKYVNKIKPYVDATSVYQQMNEDFIAMVFNCVLEASSLVDFVQVSWEYNLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNE9</accession>
    <name>T1GNE9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">YAP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02170222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNE9"/>
    <dbReference type="STRING" id="36166.T1GNE9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005097-RA">
        <property type="protein sequence ID" value="MESCA005097-PA"/>
        <property type="gene ID" value="MESCA005097"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131291_0_0_1"/>
    <dbReference type="OMA" id="FQSTVEI"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000005">
        <property type="entry name" value="Transcription enhancer factor-like protein egl-44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YAP binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005097-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="120" mass="13902" checksum="9CC84256D09FC368" modified="2013-10-16" version="1">MFQSTVEIKQILDKFPKSLKDLYEKGPQNAFYLVKCWADLNSEISGETGVFYGVASHYESEENVVLTCSTKVCSFGKQVVEKVETEFSRVENGRFVYRTHKSPMCEYMINFIQKLKHLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-05-03" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>T1GNG6</accession>
    <name>T1GNG6_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02184985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GNG6"/>
    <dbReference type="STRING" id="36166.T1GNG6"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005117-RA">
        <property type="protein sequence ID" value="MESCA005117-PA"/>
        <property type="gene ID" value="MESCA005117"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1005730_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005117-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="277" mass="31735" checksum="D06509B9BCC4B787" modified="2013-10-16" version="1">MPPKSSSSKKNQISWFCCEKCSTNIIIKDRNGHEKTCDKFQNGDIFSEEVLKTDFVRDGVLHTSTIEKRTFAVEELKDVSEKQLNNLVFVSEGVMKLCKFSIGQDLLVSTNADEQQVSIKLNYFGNTFRRPNSSKGSHSSSLNISNLNFKDVEKKISKILQIELRGKVVCENTKIFLDFFNKEIEIVLENFFTHLENEGLSEKMENLSLNEKFYLFTNSTRMILYEKNGVLEDLKQENVEKVKLKDIGGLEEQVRQVFETFGVALGFKNVPKVGIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP41</accession>
    <name>T1GP41_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of mRNA-decapping protein 4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02035299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP41"/>
    <dbReference type="STRING" id="36166.T1GP41"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005357-RA">
        <property type="protein sequence ID" value="MESCA005357-PA"/>
        <property type="gene ID" value="MESCA005357"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_931577_0_0_1"/>
    <dbReference type="OMA" id="KMAVESM"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.100:FF:000001">
        <property type="entry name" value="Enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.100">
        <property type="entry name" value="conserved c-terminal region of ge- 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049404">
        <property type="entry name" value="EDC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044938">
        <property type="entry name" value="EDC4_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21289">
        <property type="entry name" value="EDC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Enhancer of mRNA-decapping protein 4 C-terminal" evidence="7">
        <location>
            <begin position="126"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005357-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="29257" checksum="40CD03DD8AD607B9" modified="2013-10-16" version="1">MFHQLKEAFVLGIKDFMDQFDGYLTKLQPVKESTDEILTKVNSFNELFEKVYHKQKLLTRESILESRKDLKAVEMSVVNQLSSIMKTEIRKNLEDQATSLENSMLNVVRSQAQTPAPSIYDVQEQIKALLHQGHINKAFHQALIANDLALVEFTLDKADYKEVFNPCCLEQTVLLSLIQQISADMNNHNDIKQKYLSDSILNLDLTDSITREHAPKVLTELYKNCQSYLKLLPKSTLFNNVRMIMMAIQGMGVMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>T1GP67</accession>
    <name>T1GP67_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02060876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GP67"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005384-RA">
        <property type="protein sequence ID" value="MESCA005384-PA"/>
        <property type="gene ID" value="MESCA005384"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1505156_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005384-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20263" checksum="432236BA4F836F7C" modified="2013-10-16" version="1">MKSDVCCFNRQTFAILLGILELFISSVLLVPNVYCVIFPETLTNRMLATFFPTSKSASSQLYDVVSYFVTASLVLNITSVLCASILIVGTFKRNSISILIWLFNSGLKCFIGFIGCVFITLLLILILTSEFGNETLFRTTLILIVLSPLVTGLYVYLWISILKLHQTIRNDRYFFKTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GPI1</accession>
    <name>T1GPI1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02180671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GPI1"/>
    <dbReference type="STRING" id="36166.T1GPI1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005511-RA">
        <property type="protein sequence ID" value="MESCA005511-PA"/>
        <property type="gene ID" value="MESCA005511"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_1_1"/>
    <dbReference type="OMA" id="KGCIVEE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005511-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="113" mass="12666" checksum="B6CE413175C82B94" modified="2013-10-16" version="1">MIKGVTLGIQYQMRAVYAHFPINYVTSEQNTVIEIRNFLGEKYIRRVEMAKGVTVVNSTAQKDELIIEGNDIEAVSGSAALIQQSTTVKNKDIRKFLDGLYVSEKTTVVKPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQ75</accession>
    <name>T1GQ75_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02391994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02391995">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQ75"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005776-RA">
        <property type="protein sequence ID" value="MESCA005776-PA"/>
        <property type="gene ID" value="MESCA005776"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2040729_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005776-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="121" mass="13355" checksum="773A41A2DE848CEC" modified="2013-10-16" version="1">MTSNGVLPMTSILSAYPSIWQDLLKIMLPVSMRPYTIPSTYPHIGVHFISVLRLSPFVDRGLVHEGSSETAHVRAHHVLRVSTPFLLPKYQIPLLKSILLRGGQETRFGTCGQITTEGSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GQI5</accession>
    <name>T1GQI5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02103676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02103677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GQI5"/>
    <dbReference type="STRING" id="36166.T1GQI5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA005893-RA">
        <property type="protein sequence ID" value="MESCA005893-PA"/>
        <property type="gene ID" value="MESCA005893"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2164697_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="1">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA005893-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="111" mass="12455" checksum="C203446740AF24FF" modified="2013-10-16" version="1">MLMVVWTNDAYNVWFQPFTHDERKDLVGDIEQAYASLVETDDYGCSGALINDMHVLTAAHCVQGTVFREKGGLKNVRLGEFNVRTDPDCIEEPNFLNCGDSALNVGVDKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GR74</accession>
    <name>T1GR74_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02169730">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GR74"/>
    <dbReference type="STRING" id="36166.T1GR74"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006150-RA">
        <property type="protein sequence ID" value="MESCA006150-PA"/>
        <property type="gene ID" value="MESCA006150"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019036_0_0_1"/>
    <dbReference type="OMA" id="HCEENEN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040793">
        <property type="entry name" value="CDH1_2_SANT_HL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18375">
        <property type="entry name" value="CDH1_2_SANT_HL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13907">
        <property type="entry name" value="CHD1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23588">
        <property type="entry name" value="HTH_CHD1_Hrp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01176">
        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="21"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="262"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="499"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="689"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006150-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="702" mass="80277" checksum="9048F60183D0E1C5" modified="2013-10-16" version="1">MGLNQDLALQRLLKGSGKLVLLDKLLCRLKETGHRVLIFSQMVRMLDILAEYLQKRHWPFQRLDGSIKGEIRRQALDHFNAENSQDFCFLLSTRAGGLGINLATADTVIIFDSDWNPQNDLQAQARAHRIGQKNQVNIYRLVTARSVEEEIVERAKQKMVLDHLVIQRMDTTGRTVLDKNNGGAKNNTTPFNKDDLSAILKFGAEELFKDDQDGEGGDELICDIDDILRRAELRNDDPAMVGDDLLSAFKVAKMKDLYLPPRRQVINKKDNRDSDAHSDGGDGSEFSQDDDPDDSKPKKRGRPTMKEKIKGFNDTEIRKFIKSYKKFPNPLERLEDIACDAELQEKPLNDLRYLGNLLHERCLEFVKNLSNDPGAEQNISVKIGGVSFNAKTVVSSEEELSSLAQILPKDRNERLSWTLDLNTRPANFDIEWDVKDDSKLLCGINQYGIGSWESIKVDPHLNLSDKILPVNLKKKPQSKHLQSRSEYLLRIIKKNLESNKNNSNKSSETAEKVASSVEVTTKSSTDNAKKKKSKKNKKNVIDKPMHFTANNEPRALEVLGDLDPSIFNECKEKMRPVKKSLKALDKPDSNLSSQQLVDHTRQCLLSIGKQIDVCLKEYKDPDRIKEWRSNLWYFVSKFTEFDAKKLFKLYKHALKKVDKCENDKTSKRKNMVNDNSQQTSDTNFKRKRLESENKDLNSSKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GRS9</accession>
    <name>T1GRS9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02136844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02136847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="36166.T1GRS9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006369-RA">
        <property type="protein sequence ID" value="MESCA006369-PA"/>
        <property type="gene ID" value="MESCA006369"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451122_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="2" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006369-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20908" checksum="3079638419535774" modified="2013-10-16" version="1">MLPPQLEILNLLNGIYLRIQTKFTRKKNIQGTNTSASLIYCRYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDNEKQRQELEKEMEALQGDGVSNSGSPSAASAAAAAAAKKKKEEEQKKEEEEEVTEGPKPMLPYSSMXXEERRGTKERGGGRGDRRTKTNVAVFIYVYSFTNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2023-02-22" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS00</accession>
    <name>T1GS00_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02012860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS00"/>
    <dbReference type="STRING" id="36166.T1GS00"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006447-RA">
        <property type="protein sequence ID" value="MESCA006447-PA"/>
        <property type="gene ID" value="MESCA006447"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399488_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="195" mass="22422" checksum="61C2A5CAB9BDA401" modified="2013-10-16" version="1">QFNKLFSNCPVENSSGVFELNLKSQEAILSIGVYFIDSNFQYEEIILKYLLRISKSLPKAVWVDPPKWLDKDKIPTSEKFAFQFNSLLSQIIIHRPDLKDIILSSQVETLTVLSNIIKSSIDGKTICTPMILCKYIVPMFLGYCRSIGRTTPQLFLDNYDEKLKDNLFTRKFDCNLYPKVPKTETSVPAIENKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>T1GS90</accession>
    <name>T1GS90_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02130315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GS90"/>
    <dbReference type="STRING" id="36166.T1GS90"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006540-RA">
        <property type="protein sequence ID" value="MESCA006540-PA"/>
        <property type="gene ID" value="MESCA006540"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1091046_0_0_1"/>
    <dbReference type="OMA" id="HINHGIN"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052220">
        <property type="entry name" value="METTL25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496">
        <property type="entry name" value="CGI-41 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496:SF0">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="255" mass="29537" checksum="3A3816B4B9B4A2B0" modified="2013-10-16" version="1">MNFYPESKDDILYKSEFITTKSENKIEECLKDLNAEESPIGIIGLHSCADLTVTSIRIFLQMERVRKLIVMPCCYHKLKMSDGKFENFPLSTKLQKNYCGDFLNRPFLRLACQETASRWCVMSNEDHINHGINMFQRAALEVLLEKGGTFKKNKMSKISRDITESYKIECENIENLKNEYTMMLENFGSSEFVAEILTCLQATIQRVCENLVLNDRIVFMREVAIERNIPLNVSLRKIVDDTLSPRCFAFIAQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT04</accession>
    <name>T1GT04_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcriptional repressor p66 coiled-coil MBD2-interaction domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02058918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT04"/>
    <dbReference type="STRING" id="36166.T1GT04"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006820-RA">
        <property type="protein sequence ID" value="MESCA006820-PA"/>
        <property type="gene ID" value="MESCA006820"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109337_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040386">
        <property type="entry name" value="P66"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032346">
        <property type="entry name" value="P66_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455:SF7">
        <property type="entry name" value="SIMJANG, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16563">
        <property type="entry name" value="P66_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional repressor p66 coiled-coil MBD2-interaction" evidence="7">
        <location>
            <begin position="50"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006820-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="251" mass="26903" checksum="DB5340D91A0E8787" modified="2013-10-16" version="1">RTEPKSYAETPDIVLLPARVNGGRQNGNIDSETDDEEMPLSIPIKELTPSELKEREKELRKLREDLRNEETKLVLLKKLKQSQHIMKENLIVTPTNNVVVSPTNPLASISVSLAKGSLSVTPTSALPLPAHSRNQRSSNNISNSSSRNQTSFSKSNSSSGLPPPRSSLPGGATLTPGGSSSLRNSNILPRTGSNLTITPSVTITPTTAPPSGMKARNQLNVSDNSKVPQTINNSVSITPAPPILHQNDSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2022-12-14" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>T1GT68</accession>
    <name>T1GT68_MEGSC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02003727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GT68"/>
    <dbReference type="EnsemblMetazoa" id="MESCA006890-RA">
        <property type="protein sequence ID" value="MESCA006890-PA"/>
        <property type="gene ID" value="MESCA006890"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2725116_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA006890-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="72" mass="8497" checksum="E1AFDC87E99D3ED6" modified="2013-10-16" version="1">MWSIENLTDLRLVITMPYQNIVVEKMESILPARSNSSMLEFTPCLMDGSSKLRREETREKRLKTGRQFMLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTQ1</accession>
    <name>T1GTQ1_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RabBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02089982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTQ1"/>
    <dbReference type="STRING" id="36166.T1GTQ1"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007090-RA">
        <property type="protein sequence ID" value="MESCA007090-PA"/>
        <property type="gene ID" value="MESCA007090"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117933_0_0_1"/>
    <dbReference type="OMA" id="TCPNDRE"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061669">
        <property type="term" value="P:spontaneous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF6">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="6">
        <location>
            <begin position="35"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="5">
        <location>
            <begin position="83"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007090-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18727" checksum="895E331B42CA75AF" modified="2013-10-16" version="1">MTYYFNKEEENKFVCPSDRQLSLRAELKTGWSCRKTNNNPLDPAEQEAILQVIRRNEDIESTERERISKLVDRVEQMKQRVVDLGPNNCRFCGTAFNIFTSSRILCNQCHSSVCSKCIINVSSKYAKTPMYLCRICLETREMLKKTGAWFFKGLSGYQVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>T1GTX2</accession>
    <name>T1GTX2_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02096954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02096955">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GTX2"/>
    <dbReference type="STRING" id="36166.T1GTX2"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007169-RA">
        <property type="protein sequence ID" value="MESCA007169-PA"/>
        <property type="gene ID" value="MESCA007169"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1827519_0_0_1"/>
    <dbReference type="OMA" id="YACNMEL"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007169-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16638" checksum="D335EEF63D6A2ED6" modified="2013-10-16" version="1">MEALKYLFYVALLVVSLFFYVFRRRLSYWKDRGIPHDHPHIIYGNMVGIQTEKNFHEILREYYMKFKNSKPFVGFYMMASPDAMITDLDLIKNSYYACNMELSGVSKGRTVIRDTSQRGVISPLLWLLVVENLSNKQERPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUC5</accession>
    <name>T1GUC5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrator complex subunit 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02047274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02047276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUC5"/>
    <dbReference type="STRING" id="36166.T1GUC5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007335-RA">
        <property type="protein sequence ID" value="MESCA007335-PA"/>
        <property type="gene ID" value="MESCA007335"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848070_0_0_1"/>
    <dbReference type="OMA" id="PECAIKS"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160232">
        <property type="term" value="C:INTAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090101">
        <property type="term" value="P:negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090287">
        <property type="term" value="P:regulation of cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160240">
        <property type="term" value="P:RNA polymerase II transcription initiation surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000003">
        <property type="entry name" value="Integrator complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027074">
        <property type="entry name" value="Integrator_9su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-Casp" evidence="7">
        <location>
            <begin position="43"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="328" mass="36761" checksum="1B07EEFEED32B928" modified="2013-10-16" version="1">MTGLTQAPTVNPDTKLGELCINVAMTIRNNGSCLIPCYPSGVVYDLFECLTQNLENSGLANVPMFFISPVADSSLAYSNILAEWLSSAKQNKVYVPDDPFPHAFLLKNNKLKHYKHIYSEGFSTDFRQPCVVFCGHPSLRFGDAVHFIEMWGNNPNNSIIFTEPDFPYLQALAPYQPLAMKACYCPIETSLNFQQANKLIKDLKPGVLVIPEQYTKAHPSTPTLCIDQPQDKKVITFKCGEIIKLPLKRKMDRVYLSSELAQKIVPKEVVSGVNLSTLTGILHVKDNVSQIVPCDDKDDSGIPYKCPSREDLLKKVKYEYGNLDIDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUC8</accession>
    <name>T1GUC8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">sn-1-specific diacylglycerol lipase ABHD11</fullName>
            <ecNumber evidence="3">3.1.1.116</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha/beta hydrolase domain-containing protein 11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1,2-didecanoylglycerol + H2O = decanoylglycerol + decanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48596"/>
            <dbReference type="ChEBI" id="CHEBI:11152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:90605"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + H2O = 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77107"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:77129"/>
            <dbReference type="ChEBI" id="CHEBI:186738"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + H2O = 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
            <dbReference type="ChEBI" id="CHEBI:75468"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,3-diacyl-sn-glycerol + H2O = a 1-acyl-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38503"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:64683"/>
            <dbReference type="ChEBI" id="CHEBI:77272"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="3"/>
    <dbReference type="EMBL" id="CAQQ02083324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUC8"/>
    <dbReference type="STRING" id="36166.T1GUC8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007338-RA">
        <property type="protein sequence ID" value="MESCA007338-PA"/>
        <property type="gene ID" value="MESCA007338"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507094_0_0_1"/>
    <dbReference type="OMA" id="NWDSANT"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="12">
        <location>
            <begin position="12"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007338-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="179" mass="19836" checksum="9970476739DD5E25" modified="2013-10-16" version="1">LAFDVYGGGVAPPLLIFHGLFGSKNNWKEIGKTLKAILQPTRKIYAIDLRNHGQSYRSPNMTVKQLAEDIKDFIEEEGLRKVCILGYNAGGKAATHFVLENPNLVQKAIIVDSSPIESRAEAIGLDKVVLLMKNTRIPKEKTFSEGRQMALTMLQKVITDNHTANYVLSNLKKSPTDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUD9</accession>
    <name>T1GUD9_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="CAQQ02170485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02170488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUD9"/>
    <dbReference type="STRING" id="36166.T1GUD9"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007349-RA">
        <property type="protein sequence ID" value="MESCA007349-PA"/>
        <property type="gene ID" value="MESCA007349"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441664_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057032">
        <property type="entry name" value="MBTPS1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF7">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23090">
        <property type="entry name" value="MBTPS1_4th"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="BEN" evidence="3">
        <location>
            <begin position="334"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007349-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="619" mass="70300" checksum="23DF69A716AC18DB" modified="2013-10-16" version="1">MAGPDNKEARNSTVSFPIRVKIIPRPPRNKRILWDQYHSLRYPPGYLPRDNLKTKSDPLDWRADHIHTNFKDMYTHLRSAGYYIEVLGSPFTCFNASNYGTLLIVDPEEEFFDEELAKLKNDVFNHDLSVIIFADWYNTTVMKKIKFYDENTRQWWVPDTGGANIPALNDLLRHYGISLSDFVAEGYYALGDHSMYFASGTTISKFPKLNENLFPSRTKKGHCLIGLGTVNFITLNMSIQEGYVQYTDASTQTDGDSILYAVSGTFLNDYRRLREIVESHENILAGLRQSNIPTVEVSKNVSPTLQIRQTVIDDVPQVHLDTKSDSKQLLISLGPNNTIIPLQFFQNIDWSAPSKATRALLSCVFGEFVLATSTMTGKQSPAFPNNAKKEKLDTKKILKTSDSKTRSRHSVPILGILQVDKSLNNLYIDGEEFKNSLENSIDQPNQLDERNEEVIEDLEKNPILNKRILLENIESEIHNLTKERILNFDKNDIIKKLERFDDAANEVDTDLNNVDTGVGGSAVDAAAVLNEEEDPEANAKNIAQDVKFQKRSEGRIAVFGDSNCLDSTHLDKPCYWLLDALLEYTMTNHISNLLKDLNKFNKLTGNDNSYIDEKRKQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>T1GUF8</accession>
    <name>T1GUF8_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein charybde</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibits cell growth by regulating the Tor pathway upstream of the Tsc1-Tsc2 complex and downstream of Akt1. Acts as a cell death activator during head development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDIT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CAQQ02050234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02050235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GUF8"/>
    <dbReference type="STRING" id="36166.T1GUF8"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007370-RA">
        <property type="protein sequence ID" value="MESCA007370-PA"/>
        <property type="gene ID" value="MESCA007370"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086145_0_0_1"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008258">
        <property type="term" value="P:head involution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045926">
        <property type="term" value="P:negative regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.40:FF:000003">
        <property type="entry name" value="Charybde, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF16">
        <property type="entry name" value="PROTEIN CHARYBDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007370-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23192" checksum="9FC5D37398043A3A" modified="2013-10-16" version="1">MEISLPNQEQYVIGSTNKKDWKSSLTIPLPKNSSSCHKKSKCAQEKCENKSSISRNTTDSLYNFNIVYNIFDDSERIAAINSICSRLQNELRAAKSRNLDCTEVLLPDDLLTKIAAEIYNLSEKEPCGLKGCAIFIGFEDEYANMRRIASLKMDPNTLSTFELYLVIKQDGSGWHNILPQFLKNLAKSSTIVISSNYELTKTKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>T1GVU5</accession>
    <name>T1GVU5_MEGSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Megaselia scalaris</name>
        <name type="common">Humpbacked fly</name>
        <name type="synonym">Phora scalaris</name>
        <dbReference type="NCBI Taxonomy" id="36166"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Platypezoidea</taxon>
            <taxon>Phoridae</taxon>
            <taxon>Megaseliini</taxon>
            <taxon>Megaselia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hughes D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Durham, NC isolate 2 -- Noor lab</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2015-06" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CAQQ02114986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAQQ02114987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="T1GVU5"/>
    <dbReference type="STRING" id="36166.T1GVU5"/>
    <dbReference type="EnsemblMetazoa" id="MESCA007912-RA">
        <property type="protein sequence ID" value="MESCA007912-PA"/>
        <property type="gene ID" value="MESCA007912"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020233_1_0_1"/>
    <dbReference type="OMA" id="ILELHAW"/>
    <dbReference type="Proteomes" id="UP000015102">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000082">
        <property type="entry name" value="Leucine-rich repeat-containing 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473:SF10">
        <property type="entry name" value="LD45603P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004577697" description="Ig-like domain-containing protein" evidence="15">
        <location>
            <begin position="22"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="237"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="573"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="653"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="578"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="595"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="687"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="MESCA007912-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000015102"/>
        </source>
    </evidence>
    <sequence length="700" mass="76953" checksum="27FD138C64D163AC" modified="2013-10-16" version="1">MSYEIFLTVLALTSITGISLGCPAEVCTCKWKGGKQTVECGGQQLQKIPETMDPGTQVLNFSGNSLQVIQSERFYKMELVNLQKIYFAHNKLIRIHKDAFRGLSNLVELDLSDNLLQTIPNDTFPDYTSLMRLSLSGNPIREIRSSSFKSLAYLTTLELSNCQIETIENEAFVGMDNLEWLRLDGNRINFILGDNILPKSLHGISLHNNRGAKMYEPPRLKGQTIKSLKKDELACQPEVMPKSSYTEISEGRNISIVCVVRAIPEPKVLWYFNGMALTNDSFYENLHMYYYIDDSRIGEKHSEVYIYNVGLEDNGTFSCVGQNVAGTTSSNYTLRVIVKEPPIVKEVSFSKDYMNYIIASSGCGGVIFILLVCTLAIKCHTKKSKAKSGDNVKLQEPPNTGLLKCPSIITEEEMSPKSLQKENGSVILTTTQMKQNLIMYAPSNMPPMGLQQGLLLDTVNMQLSSTPPSQQLNGNMRSYQDKNPDLINDAESGHYHNNNSTDNANYDAITYGARYSLEAEFLQRANTPPYDKFLPSNVRFTPSSEGYTMVQYPITSHCIPAFSHPTQPIRYPISILTAPPPSSSPASTPPPPPSAASTIPTSTSSLLSSSSSNVNNKRCVGAQTTDVMDLPSPPPPLISTTATTTIQECEEENDIALSNNSNTKLRHLSEPLADSPDEGYVG
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